LOCUS       DAVDWT010000001       118990 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVDWT010000001 DAVDWT010000000
VERSION     DAVDWT010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 118990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 118990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..118990
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.001"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(66..1097)
                     /locus_tag="VUW50_000001"
     CDS             complement(66..1097)
                     /locus_tag="VUW50_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET7981984.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLRLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDIWYEERTDFSPTALGTPRVSHTNTGFDLLQGRAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIIYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(1352..2035)
                     /locus_tag="VUW50_000002"
     CDS             complement(1352..2035)
                     /locus_tag="VUW50_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HET7981985.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(2047..2991)
                     /locus_tag="VUW50_000003"
     CDS             complement(2047..2991)
                     /locus_tag="VUW50_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET7981986.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            3123..5954
                     /gene="uvrA"
                     /locus_tag="VUW50_000004"
     CDS             3123..5954
                     /gene="uvrA"
                     /locus_tag="VUW50_000004"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET7981987.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAITQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            5947..7008
                     /locus_tag="VUW50_000005"
     CDS             5947..7008
                     /locus_tag="VUW50_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET7981988.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMNLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLTQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            7140..7538
                     /gene="spx"
                     /locus_tag="VUW50_000006"
     CDS             7140..7538
                     /gene="spx"
                     /locus_tag="VUW50_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HET7981989.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            7604..8173
                     /locus_tag="VUW50_000007"
     CDS             7604..8173
                     /locus_tag="VUW50_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET7981990.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLVGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            8260..8526
                     /locus_tag="VUW50_000008"
     CDS             8260..8526
                     /locus_tag="VUW50_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HET7981991.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            8530..8949
                     /gene="ruvX"
                     /locus_tag="VUW50_000009"
     CDS             8530..8949
                     /gene="ruvX"
                     /locus_tag="VUW50_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET7981992.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            8965..9270
                     /locus_tag="VUW50_000010"
     CDS             8965..9270
                     /locus_tag="VUW50_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HET7981993.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            9525..10775
                     /locus_tag="VUW50_000011"
     CDS             9525..10775
                     /locus_tag="VUW50_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET7981994.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            10772..11314
                     /locus_tag="VUW50_000012"
     CDS             10772..11314
                     /locus_tag="VUW50_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HET7981995.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            11460..12992
                     /gene="cls"
                     /locus_tag="VUW50_000013"
     CDS             11460..12992
                     /gene="cls"
                     /locus_tag="VUW50_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HET7981996.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIMEIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            13070..13213
                     /locus_tag="VUW50_000014"
     CDS             13070..13213
                     /locus_tag="VUW50_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7981997.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            13250..14788
                     /locus_tag="VUW50_000015"
     CDS             13250..14788
                     /locus_tag="VUW50_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET7981998.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     YFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFVHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRGVRRSRRETPVSLWSRFFKGNLYLLVFGLVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDTIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            14908..17115
                     /gene="nrdD"
                     /locus_tag="VUW50_000016"
     CDS             14908..17115
                     /gene="nrdD"
                     /locus_tag="VUW50_000016"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HET7981999.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            17127..17267
                     /locus_tag="VUW50_000017"
     CDS             17127..17267
                     /locus_tag="VUW50_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982000.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            17311..17817
                     /locus_tag="VUW50_000018"
     CDS             17311..17817
                     /locus_tag="VUW50_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7982001.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDTENFVYEEWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDAHNGVER
                     YWIEVANE"
     gene            17810..18400
                     /gene="nrdG"
                     /locus_tag="VUW50_000019"
     CDS             17810..18400
                     /gene="nrdG"
                     /locus_tag="VUW50_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HET7982002.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            18397..18981
                     /locus_tag="VUW50_000020"
     CDS             18397..18981
                     /locus_tag="VUW50_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HET7982003.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVD"
     gene            19281..19589
                     /gene="rpsJ"
                     /locus_tag="VUW50_000021"
     CDS             19281..19589
                     /gene="rpsJ"
                     /locus_tag="VUW50_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET7982004.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            19806..20432
                     /gene="rplC"
                     /locus_tag="VUW50_000022"
     CDS             19806..20432
                     /gene="rplC"
                     /locus_tag="VUW50_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET7982005.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            20457..21080
                     /gene="rplD"
                     /locus_tag="VUW50_000023"
     CDS             20457..21080
                     /gene="rplD"
                     /locus_tag="VUW50_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET7982006.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            21080..21376
                     /locus_tag="VUW50_000024"
     CDS             21080..21376
                     /locus_tag="VUW50_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET7982007.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            21394..22227
                     /gene="rplB"
                     /locus_tag="VUW50_000025"
     CDS             21394..22227
                     /gene="rplB"
                     /locus_tag="VUW50_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET7982008.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            22331..22612
                     /gene="rpsS"
                     /locus_tag="VUW50_000026"
     CDS             22331..22612
                     /gene="rpsS"
                     /locus_tag="VUW50_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET7982009.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            22624..22968
                     /gene="rplV"
                     /locus_tag="VUW50_000027"
     CDS             22624..22968
                     /gene="rplV"
                     /locus_tag="VUW50_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET7982010.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            22981..23634
                     /gene="rpsC"
                     /locus_tag="VUW50_000028"
     CDS             22981..23634
                     /gene="rpsC"
                     /locus_tag="VUW50_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET7982011.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            23638..24051
                     /gene="rplP"
                     /locus_tag="VUW50_000029"
     CDS             23638..24051
                     /gene="rplP"
                     /locus_tag="VUW50_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET7982012.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            24061..24267
                     /locus_tag="VUW50_000030"
     CDS             24061..24267
                     /locus_tag="VUW50_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET7982013.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            24292..24552
                     /gene="rpsQ"
                     /locus_tag="VUW50_000031"
     CDS             24292..24552
                     /gene="rpsQ"
                     /locus_tag="VUW50_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET7982014.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            24578..24946
                     /gene="rplN"
                     /locus_tag="VUW50_000032"
     CDS             24578..24946
                     /gene="rplN"
                     /locus_tag="VUW50_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET7982015.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            25024..25329
                     /gene="rplX"
                     /locus_tag="VUW50_000033"
     CDS             25024..25329
                     /gene="rplX"
                     /locus_tag="VUW50_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET7982016.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            25353..25895
                     /gene="rplE"
                     /locus_tag="VUW50_000034"
     CDS             25353..25895
                     /gene="rplE"
                     /locus_tag="VUW50_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET7982017.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            25913..26182
                     /gene="rpsN"
                     /locus_tag="VUW50_000035"
     CDS             25913..26182
                     /gene="rpsN"
                     /locus_tag="VUW50_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HET7982018.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            26467..26865
                     /gene="rpsH"
                     /locus_tag="VUW50_000036"
     CDS             26467..26865
                     /gene="rpsH"
                     /locus_tag="VUW50_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET7982019.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            27057..27593
                     /gene="rplF"
                     /locus_tag="VUW50_000037"
     CDS             27057..27593
                     /gene="rplF"
                     /locus_tag="VUW50_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET7982020.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            27677..28033
                     /gene="rplR"
                     /locus_tag="VUW50_000038"
     CDS             27677..28033
                     /gene="rplR"
                     /locus_tag="VUW50_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET7982021.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            28051..28545
                     /gene="rpsE"
                     /locus_tag="VUW50_000039"
     CDS             28051..28545
                     /gene="rpsE"
                     /locus_tag="VUW50_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET7982022.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            28517..28741
                     /gene="rpmD"
                     /locus_tag="VUW50_000040"
     CDS             28517..28741
                     /gene="rpmD"
                     /locus_tag="VUW50_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET7982023.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            28886..29326
                     /gene="rplO"
                     /locus_tag="VUW50_000041"
     CDS             28886..29326
                     /gene="rplO"
                     /locus_tag="VUW50_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET7982024.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            29339..30649
                     /gene="secY"
                     /locus_tag="VUW50_000042"
     CDS             29339..30649
                     /gene="secY"
                     /locus_tag="VUW50_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET7982025.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            30800..31438
                     /locus_tag="VUW50_000043"
     CDS             30800..31438
                     /locus_tag="VUW50_000043"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET7982026.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGVINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            31555..31773
                     /gene="infA"
                     /locus_tag="VUW50_000044"
     CDS             31555..31773
                     /gene="infA"
                     /locus_tag="VUW50_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET7982027.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            31798..31914
                     /gene="rpmJ"
                     /locus_tag="VUW50_000045"
     CDS             31798..31914
                     /gene="rpmJ"
                     /locus_tag="VUW50_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET7982028.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            31932..32297
                     /gene="rpsM"
                     /locus_tag="VUW50_000046"
     CDS             31932..32297
                     /gene="rpsM"
                     /locus_tag="VUW50_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET7982029.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            32315..32698
                     /gene="rpsK"
                     /locus_tag="VUW50_000047"
     CDS             32315..32698
                     /gene="rpsK"
                     /locus_tag="VUW50_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET7982030.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            32741..33676
                     /locus_tag="VUW50_000048"
     CDS             32741..33676
                     /locus_tag="VUW50_000048"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET7982031.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            33688..34074
                     /gene="rplQ"
                     /locus_tag="VUW50_000049"
     CDS             33688..34074
                     /gene="rplQ"
                     /locus_tag="VUW50_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET7982032.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            34339..34605
                     /locus_tag="VUW50_000050"
     CDS             34339..34605
                     /locus_tag="VUW50_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET7982033.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            34615..35952
                     /locus_tag="VUW50_000051"
     CDS             34615..35952
                     /locus_tag="VUW50_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HET7982034.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAATDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMRVNGASSVDFISRGGQIPAPIHSFKN"
     gene            36196..36888
                     /locus_tag="VUW50_000052"
     CDS             36196..36888
                     /locus_tag="VUW50_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7982035.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(36991..38626)
                     /locus_tag="VUW50_000053"
                     /pseudo
     CDS             complement(36991..38626)
                     /locus_tag="VUW50_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(38616..39707)
                     /locus_tag="VUW50_000054"
     CDS             complement(38616..39707)
                     /locus_tag="VUW50_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982036.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNLSVLLMDEP
                     LSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPTNEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKDNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            complement(39720..40736)
                     /locus_tag="VUW50_000055"
     CDS             complement(39720..40736)
                     /locus_tag="VUW50_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7982037.1"
                     /translation="MKKKWMYYAACSSNESADDSSSDKGDGGSLVVYSPNSEGLIGAT
                     IPAFEEKYGIKIELIQAGTGELFKKLESEKEVPVADVIFGGSYTQYATHGELFENYTS
                     KENDNVIKEYQNTTGYSTPYTLDGSVLIVNPDLTKGMNIEGYNDLLKPELKGKIATAD
                     PANSSSAFAQLTNMLQAQGGYKDDKAWSYVKDLFTLIDGKIGSSSSSVYKAVADGEMA
                     VGLSYEDPAVKLLNDGANIKVVYPKEGTVFLPASAAIVKKAKNMENAKKFIDFIISQE
                     VQDTLGTTTTNRPVRKNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDI
                     QSKQ"
     gene            complement(40953..41150)
                     /locus_tag="VUW50_000056"
     CDS             complement(40953..41150)
                     /locus_tag="VUW50_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET7982038.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(41225..42001)
                     /locus_tag="VUW50_000057"
     CDS             complement(41225..42001)
                     /locus_tag="VUW50_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HET7982039.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            42123..42869
                     /locus_tag="VUW50_000058"
     CDS             42123..42869
                     /locus_tag="VUW50_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET7982040.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     RQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            42882..43862
                     /locus_tag="VUW50_000059"
     CDS             42882..43862
                     /locus_tag="VUW50_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HET7982041.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGVLQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            44057..44377
                     /locus_tag="VUW50_000060"
     CDS             44057..44377
                     /locus_tag="VUW50_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET7982042.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            44423..44731
                     /locus_tag="VUW50_000061"
     CDS             44423..44731
                     /locus_tag="VUW50_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET7982043.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            44748..46046
                     /locus_tag="VUW50_000062"
     CDS             44748..46046
                     /locus_tag="VUW50_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET7982044.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSWYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            46197..48635
                     /locus_tag="VUW50_000063"
     CDS             46197..48635
                     /locus_tag="VUW50_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HET7982045.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIGGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKAALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPKTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            48654..49322
                     /locus_tag="VUW50_000064"
     CDS             48654..49322
                     /locus_tag="VUW50_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HET7982046.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            49340..50428
                     /locus_tag="VUW50_000065"
     CDS             49340..50428
                     /locus_tag="VUW50_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HET7982047.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            50883..53384
                     /locus_tag="VUW50_000066"
     CDS             50883..53384
                     /locus_tag="VUW50_000066"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET7982048.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETDESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            53578..54273
                     /locus_tag="VUW50_000067"
     CDS             53578..54273
                     /locus_tag="VUW50_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7982049.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            54285..54701
                     /locus_tag="VUW50_000068"
     CDS             54285..54701
                     /locus_tag="VUW50_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7982050.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEEIERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(55516..55608)
                     /locus_tag="VUW50_000069"
     CDS             complement(55516..55608)
                     /locus_tag="VUW50_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HET7982051.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            55769..56740
                     /gene="ruvB"
                     /locus_tag="VUW50_000070"
     CDS             55769..56740
                     /gene="ruvB"
                     /locus_tag="VUW50_000070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET7982052.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            56721..57293
                     /locus_tag="VUW50_000071"
     CDS             56721..57293
                     /locus_tag="VUW50_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET7982053.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            57524..58282
                     /locus_tag="VUW50_000072"
     CDS             57524..58282
                     /locus_tag="VUW50_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET7982054.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            58291..59094
                     /locus_tag="VUW50_000073"
     CDS             58291..59094
                     /locus_tag="VUW50_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET7982055.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            59116..60375
                     /gene="rseP"
                     /locus_tag="VUW50_000074"
     CDS             59116..60375
                     /gene="rseP"
                     /locus_tag="VUW50_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET7982056.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPKDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            60388..62241
                     /locus_tag="VUW50_000075"
     CDS             60388..62241
                     /locus_tag="VUW50_000075"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET7982057.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKATDGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            62340..63719
                     /gene="bglA"
                     /locus_tag="VUW50_000076"
     CDS             62340..63719
                     /gene="bglA"
                     /locus_tag="VUW50_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET7982058.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNTQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSALGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQKRRLKKSGHWF
                     KELSDNNGF"
     gene            63911..65719
                     /gene="glmS"
                     /locus_tag="VUW50_000077"
     CDS             63911..65719
                     /gene="glmS"
                     /locus_tag="VUW50_000077"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET7982059.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVVDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEVKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVSTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            65838..66887
                     /locus_tag="VUW50_000078"
     CDS             65838..66887
                     /locus_tag="VUW50_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7982060.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(66987..70850)
                     /gene="spuA"
                     /locus_tag="VUW50_000079"
     CDS             complement(66987..70850)
                     /gene="spuA"
                     /locus_tag="VUW50_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HET7982061.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPGNANASLEKTEEKPATEPP
                     TPAASPADPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQ
                     GLWTWDDVEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNL
                     TGDKSVEKLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVK
                     NPSSAQWPDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFT
                     DLKNHSQIFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKH
                     SNITNHLGNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDE
                     TYSYDGKLGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTW
                     KQTLDSTNKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSNDAKIDDAHKVA
                     KAAFVDPAKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEA
                     FIEKLDYLKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSL
                     TGMYSSDPKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFM
                     DADGTPRTSFGGGRLGTTHHMTKRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEA
                     YKAARALNPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGY
                     PNEGQPAFITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSI
                     KKDPSKAENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDK
                     VPNKSHLLRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKG
                     LIALRQSTDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVN
                     ADEKAREFNLGTAFAHLRNAEVLADENQAGSVGIANPKGLEWTEKGLKLNALTATVLR
                     VSQNGTSHESTAEEKPDSTPSKPEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENK
                     PSQATADSQAEQPAQEAQASSVKEAVRNESVENSSKENIPATPDKQAELPNTGIKNEN
                     KLLFAGISLLALLGLGFLLKNKKEN"
     gene            71267..71680
                     /gene="rpsL"
                     /locus_tag="VUW50_000080"
     CDS             71267..71680
                     /gene="rpsL"
                     /locus_tag="VUW50_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET7982062.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            71700..72170
                     /gene="rpsG"
                     /locus_tag="VUW50_000081"
     CDS             71700..72170
                     /gene="rpsG"
                     /locus_tag="VUW50_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET7982063.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            72595..74676
                     /gene="fusA"
                     /locus_tag="VUW50_000082"
     CDS             72595..74676
                     /gene="fusA"
                     /locus_tag="VUW50_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET7982064.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            74780..79171
                     /locus_tag="VUW50_000083"
     CDS             74780..79171
                     /locus_tag="VUW50_000083"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HET7982065.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VDKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFVDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            79273..79536
                     /locus_tag="VUW50_000084"
     CDS             79273..79536
                     /locus_tag="VUW50_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HET7982066.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            79529..79739
                     /locus_tag="VUW50_000085"
                     /pseudo
     CDS             79529..79739
                     /locus_tag="VUW50_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
     gene            79760..79912
                     /locus_tag="VUW50_000086"
     CDS             79760..79912
                     /locus_tag="VUW50_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982067.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            79934..81175
                     /locus_tag="VUW50_000087"
     CDS             79934..81175
                     /locus_tag="VUW50_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET7982068.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            81185..81415
                     /locus_tag="VUW50_000088"
     CDS             81185..81415
                     /locus_tag="VUW50_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET7982069.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            81638..82360
                     /locus_tag="VUW50_000089"
     CDS             81638..82360
                     /locus_tag="VUW50_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET7982070.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            82577..83911
                     /gene="pepC"
                     /locus_tag="VUW50_000090"
     CDS             82577..83911
                     /gene="pepC"
                     /locus_tag="VUW50_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HET7982071.1"
                     /translation="MNAIQESFTNKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSIKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(83967..84878)
                     /locus_tag="VUW50_000091"
     CDS             complement(83967..84878)
                     /locus_tag="VUW50_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET7982072.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(84902..85705)
                     /locus_tag="VUW50_000092"
     CDS             complement(84902..85705)
                     /locus_tag="VUW50_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HET7982073.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(85733..86722)
                     /gene="manX"
                     /locus_tag="VUW50_000093"
     CDS             complement(85733..86722)
                     /gene="manX"
                     /locus_tag="VUW50_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HET7982074.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(86955..87974)
                     /gene="adhP"
                     /locus_tag="VUW50_000094"
     CDS             complement(86955..87974)
                     /gene="adhP"
                     /locus_tag="VUW50_000094"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HET7982075.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSVRAGGRVVAVGLPSETMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(88313..89125)
                     /locus_tag="VUW50_000095"
     CDS             complement(88313..89125)
                     /locus_tag="VUW50_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET7982076.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            89315..90733
                     /locus_tag="VUW50_000096"
     CDS             89315..90733
                     /locus_tag="VUW50_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET7982077.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLVITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSLNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHVMIWILDALFILNYISMAL"
     gene            90786..91493
                     /locus_tag="VUW50_000097"
     CDS             90786..91493
                     /locus_tag="VUW50_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7982078.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            91588..92562
                     /gene="folP"
                     /locus_tag="VUW50_000098"
     CDS             91588..92562
                     /gene="folP"
                     /locus_tag="VUW50_000098"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET7982079.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAYVVAEARAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            92564..93886
                     /locus_tag="VUW50_000099"
     CDS             92564..93886
                     /locus_tag="VUW50_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET7982080.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEVIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            93867..94421
                     /gene="folE"
                     /locus_tag="VUW50_000100"
     CDS             93867..94421
                     /gene="folE"
                     /locus_tag="VUW50_000100"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET7982081.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            94464..95276
                     /gene="folK"
                     /locus_tag="VUW50_000101"
     CDS             94464..95276
                     /gene="folK"
                     /locus_tag="VUW50_000101"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET7982082.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(95426..95743)
                     /locus_tag="VUW50_000102"
     CDS             complement(95426..95743)
                     /locus_tag="VUW50_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HET7982083.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            96100..96546
                     /gene="rplM"
                     /locus_tag="VUW50_000103"
     CDS             96100..96546
                     /gene="rplM"
                     /locus_tag="VUW50_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET7982084.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            96565..96957
                     /gene="rpsI"
                     /locus_tag="VUW50_000104"
     CDS             96565..96957
                     /gene="rpsI"
                     /locus_tag="VUW50_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET7982085.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            97360..97874
                     /gene="tnpA"
                     /locus_tag="VUW50_000105"
                     /pseudo
     CDS             97360..97874
                     /gene="tnpA"
                     /locus_tag="VUW50_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775157.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(98074..98676)
                     /locus_tag="VUW50_000106"
     CDS             complement(98074..98676)
                     /locus_tag="VUW50_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003030523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7982086.1"
                     /translation="MKKTLYLMRHGQTLFNKRHLIQGWCDSPLTDFGIYQAQVASEYF
                     KEHGIIFDSAFSSTSERACDTLEIVTDGKMPYKRIKGLKEWNFGVFEGESEELNPSIP
                     YENFFVYYGGESQLELQDRINNTICSLMKEAKGNSILIVSHGAAIRNFARVWENYEKT
                     TINNRMTNCCILKFTYSNDIFYLEEVINHDFSNWNNNSIQ"
     gene            complement(98686..99526)
                     /locus_tag="VUW50_000107"
                     /pseudo
     CDS             complement(98686..99526)
                     /locus_tag="VUW50_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            complement(99623..100504)
                     /locus_tag="VUW50_000108"
     CDS             complement(99623..100504)
                     /locus_tag="VUW50_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET7982087.1"
                     /translation="MINLGQLHKNTPYKNELIPYSLTRTIMEDGLPDILFHWHTDMEI
                     IYVHEGQARFHIDTDYFNSEKGDIILIRPNALHSIHPIENTYHYMDAINFQLDLIGYS
                     TKDFAAIHYLHPLYNGQLDFKRVIKPNQPGYTEIRQTLINSMEIGYYRKTFFELELKS
                     ELLKLLHLLFQYNYVYSSKQSIDGYRREEKIRKIIDYISEHYQEDLSINLLAELCGYS
                     PTHFMNFFKKNLGVSCMDYLIHYRLRKATELLQHSTLSVLEIASSVGFSNLSNFNRQF
                     KKIYKTIPRKYRDEIDE"
     gene            100614..102050
                     /locus_tag="VUW50_000109"
     CDS             100614..102050
                     /locus_tag="VUW50_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003033146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET7982088.1"
                     /translation="MSKFPKEFLWGGATAANQYEGAYNVGDKGLSVQDVTPRGGVPVS
                     DNDPNPFITELPTEDNLKLEGIDFYHRYKEDVALFAEMGFKVFRTSIAWSRIFPNGDE
                     LEPNEEGLQFYDNLFDELAKYGIEPLVTLSHYETPLHLARKYNGWVNRDLIGFYERYV
                     RTVFNRYKNKVKYWLTFNEINSVLHVPFISGGIATPVEKLSKQDLYQAVHHELVASAL
                     ATKVGHEINPEFKIGCMVLAMPTYPMTPKPEDVLAAREFENQNYLFSDIHARGKYPKY
                     IQRFFKENDINIKFESGDKELLAENTVDFISFSYYMSSVQAHDPENYQSGIGNLLGGI
                     ANPYLESSEWGWQVDPIGLRIVLNNLYDRYQLPLFIVENGLGAKDVLIEGADGPTVDD
                     DYRIDYLKKHLQQVGEAIEDGVELLGYTTWGCIDLVSASTAQMSKRYGFIYVDRNDDG
                     SGTLNRYKKKSFYWYKEVIESNGETLYD"
     gene            complement(102151..102673)
                     /locus_tag="VUW50_000110"
                     /pseudo
     CDS             complement(102151..102673)
                     /locus_tag="VUW50_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(103007..103582)
                     /locus_tag="VUW50_000111"
     CDS             complement(103007..103582)
                     /locus_tag="VUW50_000111"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7982089.1"
                     /translation="MYHIKADKRSLASARLISQGLNRLLQAKDFDSITVTDIVNEAGV
                     GRATFYRLFDNVTDVITYSYYIIAHPTLEKIQNKFISNILDFSKSLINDFYSQDSILL
                     ISTLLFSVQNGYHFLSMLNLYKNDLLVLLKNEKNLKTKSQDYKYLMEIYLMTLMVTVG
                     SYIQKPEITRELLLEKVSNVWQDLTHLHINN"
     gene            103747..105096
                     /locus_tag="VUW50_000112"
     CDS             103747..105096
                     /locus_tag="VUW50_000112"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012587.1,HMM:NF014437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET7982090.1"
                     /translation="MSKYNVLAKDIVTYVGGAENVKSLRHCATRLRFELKDESQADKE
                     ALMNLDILQVVQASGQYQVVVGPHVASVYEAVMAELPVIGGASSINEDTTAEEKKWYD
                     VIFDTISGSFTPLIPVFCGSGLVKALGSVLLMTGLLTAESSTYAVLNAAGNAVFYFLP
                     ILLAISVANKLGTNPYIAGAIGAALLEPSFTALAGNESNTFLGLPIIINNYASSVFPA
                     FMAVVGLYFLDKFLKKVVPATIHLVVLPFIEIVAMVPLTIFIFGPFGTYLSTGLSMFV
                     TNLLDLNAMLAGAVLSMIWIYVVMLGLHWALIPIMINNIATNGSDPLMGLMIGTVWVA
                     GGVSLGVALKAKDKKLKGIAWTGMIPCFLSGVSEPIMYGILFRYKKSLMWATVMNAVT
                     GAMAGLIGISGTQIAGGVFTIPTFSPILGYLALIAVSVFGTAALIVIFGFGEEGSKA"
     gene            105110..106315
                     /locus_tag="VUW50_000113"
     CDS             105110..106315
                     /locus_tag="VUW50_000113"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982091.1"
                     /translation="MKVAYHKIPIPVQLPCHMEGYVDRVSSKIHDHLELVSFILQGET
                     TIVYHVLDVILIEKELANRLQNAINQKFQIPIEQVFIAATHTHSSVKISNYLLPSYPP
                     EAELLDKIEELVLENTAICLANLKESQANYREISTGHLYSNRNDPSHPFNDKAYAIRF
                     EDTNQQPIFELVNIACHPTVLAPEVTEISRDYVGVLRDDYEKATGVPLAVLIGEAGDV
                     STRHTRKGQDFSEVERVGKTISQLLQNEKNSHLLDLSGMHLRQKTVEFTYKPQEDQDL
                     LMTRKLYQEQLKNLEGSKQGEIISDFLRMIDKKLQKESLTLTVPISVLDCKDWQILQF
                     PSEILSDFGLKIREQSQKKQAIHAYTNGFMGYAVPEHLYGKVYESAVSEFPRGIADQL
                     IEQLITLSK"
     gene            106329..107093
                     /locus_tag="VUW50_000114"
     CDS             106329..107093
                     /locus_tag="VUW50_000114"
                     /inference="COORDINATES: protein motif:HMM:NF012958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET7982092.1"
                     /translation="MSYIQLDFHSHSLNRNVNLNIFLPDGELLPAPEQPYPTIYFLPG
                     FSASGKELSTFLHFRTQCLLNGIAVVIPDGDNSFYLDKPERLALYSQYVSQELIDYTR
                     SILPLSHKREETYIGGISMGGYGALVHGFRYPEKFGKIVALSPAIDVAAILNGNASQF
                     GTTFADLFKTEEDYYSSTDHPVNALKELLDKGEDIPELFIACGFQDELVYASNRSFAE
                     ELEKLGVNLTYIEDDGGHDVFFWDKYLPAVFEFLRK"
     gene            107169..108089
                     /locus_tag="VUW50_000115"
     CDS             107169..108089
                     /locus_tag="VUW50_000115"
                     /inference="COORDINATES: protein motif:HMM:NF019474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7982093.1"
                     /translation="MKVQTYTLNDTAHVTAYILDSQISYGVHRKRPAMIICPGGGYLT
                     LATKEGEAVATSFLSQGYHCFVLRYSTYHLERVTSLEETPIINPKARYPQPILELMQT
                     MLLLHEHAQEWHLDDDNIFVTGFSAGGHLAASLGVHWDNPDFHQILEQDFEPEDLKPK
                     GLVLAYPLLEGHGGDYIAKNTPKEHLIAHQLADMSDFFFQTQEPSQVQKEAVSILPFI
                     NEKTPPTFLWITGQDQIVDPRACLVYAIKMQELGLDCECHLFNNGPHGLARADKVYAK
                     SSTEVNPPVGVWIEMALTWLDGQMEKNHEY"
     gene            108079..108915
                     /locus_tag="VUW50_000116"
     CDS             108079..108915
                     /locus_tag="VUW50_000116"
                     /inference="COORDINATES: protein motif:HMM:NF019474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7982094.1"
                     /translation="MNIKTYRTSKERLFADYIKAEKERADKKIYSLDLPEIGRLTAYL
                     YQPEDKSRMADQPILAVFHGGGFVLGYPEQEGPYARKMAERLGCLVVVLDYKLAPEFP
                     FPLPITSSYDFLSQFMQEKSNLGVSSEELILLGHSAGGNLVLAIEQLNQERQGLHIKQ
                     VIVNYPVLDWRQWSQDKSRLGDYCHWYLGKNFSNIHYPLASPVLAEGLNKPNIFLMVA
                     SLDPLAPQAHDFFQKNPKISYKEYEGAQHGFTHFWYQEFDAEKSAEAWEDIIRFIKEG
                     QL"
     gene            108912..109655
                     /locus_tag="VUW50_000117"
     CDS             108912..109655
                     /locus_tag="VUW50_000117"
                     /inference="COORDINATES: protein motif:HMM:NF016668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HET7982095.1"
                     /translation="MKDILMRIDDLGYSEGVNYGIARAVKAGPVNHVGLMTNMPWAAH
                     GYDLIKDSGVSLGLHANISAGRPLSPLEEVGSLIVEGHFKSSSTYNQARYQDISVQDV
                     ITEIRQQVEAFKMLVGKLPDYIDVHAVFHDTFNQGVAMVAKEYGLPFVDVVAGKIHGT
                     DKTIHYLGGTSQEADLLLPTLQKQAEETEHLPLFVYHPGFIDQDLIATSSLTLQRVAD
                     VAFLTGQELRDYLEQAELHLFDIRDLEKL"
     gene            109757..111118
                     /locus_tag="VUW50_000118"
     CDS             109757..111118
                     /locus_tag="VUW50_000118"
                     /inference="COORDINATES: protein motif:HMM:NF019474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7982096.1"
                     /translation="MLKKYLSLFLGLIMTILVWAQSILINFEVPLAILTFILIIGFVF
                     WNNRKTDEQNKTLIWLGLSYILVRTIWFFFSHVSLWGLGQILLIILSIGLLIKGLHFS
                     NKNIPIQILKGLFLTFLTVITLLLTYLTVDSSPIAKLTQSVANTINSFEPQTDFTVTV
                     SEGNQLLENIQYGTELPNSYLDIILPEGDNINSRPTYIFFHGGGFVAGDKMQGDPNQV
                     SEYSASLYHFEEMVKAGYNVVTVNYALASQYPYPTAIKQLSDAVVFLEKNAEKYQLNM
                     NEVVIAGASAGGNLAAEFITIQANPSYAEELNVTPVLDSSKIKAAVLEVPALDPNRVA
                     KTHRPSIIEDYVFGHALAAYMEQSLVSPNRELYASRNLINKITADFPPVFITDGNIGS
                     FMDQAQDYAKKLAEKGVTYELYIPDEATYGDQPHGYLADIYSEATQTYIKKKISFLEK
                     LTN"
     gene            111268..111372
                     /locus_tag="VUW50_000119"
     CDS             111268..111372
                     /locus_tag="VUW50_000119"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7982097.1"
                     /translation="MYRRLSDLRDDLDIFQSEIADFLHCTQSTYSKIE"
     gene            complement(111442..111552)
                     /locus_tag="VUW50_000120"
                     /pseudo
     CDS             complement(111442..111552)
                     /locus_tag="VUW50_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004897104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            111706..111986
                     /gene="tnpA"
                     /locus_tag="VUW50_000121"
                     /pseudo
     CDS             111706..111986
                     /gene="tnpA"
                     /locus_tag="VUW50_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148688.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(112207..113370)
                     /locus_tag="VUW50_000122"
     CDS             complement(112207..113370)
                     /locus_tag="VUW50_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HET7982098.1"
                     /translation="MVENVGQLAIEQAKANGGKLEVVSKVTVNNRHDLSIAYSPGVAS
                     VSSAIAENADLAYTLTTKKNTVAVVSDGTAVLGLGDIGPAAAMPVMEGKAALFKRFAD
                     VDAVPIVLDTKDPEEIIRIVKAMAPTFGGVNLEDISAPRCFEIEQRLIEECDIPIFHD
                     DQHGTAIVVLAGLFNSLKLAQKELDKVKIVVNGGGSAGLSITRKLLAAGATDVTVVDR
                     FGIISEEDHDKLAPHHQDIAKVTNRRLASGSLADALEDADVFLGVSAPGALNPEWIAK
                     MNDKPIIFAMANPTPEIFPDEALAAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKKITVEMQIAAAKGIASLIPDSELSPTNIIPDPFQEGVAKVVAKSVSDAVKD"
     gene            complement(113412..114740)
                     /locus_tag="VUW50_000123"
     CDS             complement(113412..114740)
                     /locus_tag="VUW50_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HET7982099.1"
                     /translation="MASVKAKQQPTLDATKQSISDVKVGSVPLPVYLCLSALILAAGL
                     LQQLPVNMLGGFAVILTIGWLLGTIGASIPGLKNFGGPAILSLLVPSILVFFNLINPN
                     VLEATNVLMKQANFLYFYIACLVSGSILGMNRKMLIQGLFRMIIPMLLGMICAMAVGT
                     LVGVLLGLDWQHTLFYIVTPVLAGGIGEGILPLSIGYSAITGVSSEQLVAQLIPATII
                     GNFFAILCTALLSRLGEKKPELSGQGQLVKVSDKEDLSDILEDKSGPLDVKMMGAGVL
                     TACTLFISGHLMQHLTGFPGPVLMIVIAALMKYVNVIPQETQNGAKQLYKFISGNFTF
                     PLMAGLGLLYIPLKDVVATLSLPYFIVVISVVFTVVSVGFFVSRFLNMHPVEAAIISS
                     CQSGMGGTGDVAILSTANRMNLMPFAQVATRLGGAITVIIMTAILRMIFK"
     gene            114895..116406
                     /locus_tag="VUW50_000124"
     CDS             114895..116406
                     /locus_tag="VUW50_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7982100.1"
                     /translation="MKKNLSLWAFLSLFLIGVLTLILSIFYYETTHESYQIIRIQEER
                     FLKTAGRMIAKNQQIIAMLKQGESDQQVQDYTLALAEDSHLDYIVVMDLSGTRLTHPN
                     PSKIGESFQGGDEKAVLNGQEILSVAEGSLGESLRYIVPVYDGKQQIGAVAVGLKLTT
                     LSDISQQNIQQYTRALVISILLSLLVTSSISYFLKKRLHNLEPNEIFQNLEERNAMLE
                     QTHAAVFVLDQQHYIQRHNPAAVRLLGAYSNNKELIGERLDDLLPMLNEKHFATQNEQ
                     IVSFRNQDYLLSISPIVVQNQNRGFVVFIRNATETLLQMEQLTYTTTYASALQAQTHS
                     FMNQLHVIYGLVDIAYYDELKVYLNELLEPRNEFLTSLSMLIKDPQLASFLIGEKEKF
                     SEKHTQLQFEIRGEVGLPQIADQLHHYIFIHRYINTSLLHFTLPETIQIQLSYQDKLL
                     QTEYRFSAPTFLSDNFLASLQEPYFQQLLADADGEYEFNTQEDTIVISTRITY"
     gene            116417..117097
                     /locus_tag="VUW50_000125"
     CDS             116417..117097
                     /locus_tag="VUW50_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7982101.1"
                     /translation="MNVLIIEDDPMVEFIHRNYLEKVAIFREIYSTNSQIEAHHFLEK
                     YPIDLILLDIHIKDGNGLILLEELRAIHINAEVIAITAASEAQIVKSSFHLGVIDYLI
                     KPFTFDRFEVAIQRFLQHHKAFDEATVGQETIDHYHHKFSVANPVSHQGLDEKGLSET
                     TYQHILQAISRLKQPFTIQELADASQFSHVSVRKYVAYMEEQGELISEQIYTKVGRPF
                     KAYRKAIQ"
BASE COUNT        35679 a        21918 c        26258 g        35135 t
ORIGIN      
        1 ctgtaatatg tgatgaaaat accagtaatg ataccgagaa aaaagctgag aaacttttcc
       61 cagctttatt tgttatagtc aaagcgaatg acttgttcct gtgcatctac atgagcatgg
      121 accccaaagg gtacaattgc tcttggagtt gcgtggccga cattcagatt atagataatc
      181 gggatattgc tgtcaatgat atccaatagt gcctctttat agtcgtcatg gaaggtttca
      241 tccataggtt ttccgaccaa gagtccattg atgaccgcga atatgccagt gtcctttaaa
      301 gttagcaaca tctttttgaa gtcttctggc ttaggctttt cttcgcttgt ttcgagcaag
      361 aggattttcc cttcccagtc tgacaagtca gggaaaagtt tgtatttttg gcagagttcc
      421 gtgctatctg cgtatcgaga gttgtcaaag atatcgtaga gggattcgag gcaaccaccg
      481 aggattttcc cctcgaattg ggcccttcct tgcaacaagt caaaacctgt atttgtatga
      541 ctgacacgag gtgttcccag ggccgtggga ctaaaatcag ttcgttcctc ataccaaatg
      601 tcactagggc ggatttctga aattcttccc gtctcaatca attctttaaa gtagtgaagg
      661 ctataggcta gcatttcttt gtctaattca caaatgtctg ctaaaaagga ttgaccataa
      721 aaagtcttga ttcctaattt atgcaacatg aggtggttca tggttgtatc cgagaagcca
      781 agaaaaattt tttgtttgat aaccttttgg agttggtcat tttcaaaaag ataaggtagc
      841 aagcgatagg tatcgtctcc accgatggca cataggatca tgtcgatgct atcatcagaa
      901 aaggcatgaa tcaaatcctc tgcacgagct tcaggatggt ccttgataaa gtctaatcct
      961 tttaacgaat ggggcaaaaa gatgggattg agtctcagat ccttgagacg ttggataccc
     1021 aagtccactt cgtgtttgac aaaatcctct ccgataatgc cactagataa actaacaata
     1081 ccaatagtag aaaccatatc tcatcctcct agaaatagat tgagcctatt ttatcacaaa
     1141 atataagaga aagctatgtg ggatttcaga aaaatacttt taatactctt cgaaaatctc
     1201 ttcaaaccac gtcagtttta tttgcaacct caaagctgtg ctttgagcaa cctgcagcta
     1261 gcttcctagt ttgctctttg attttcattg agtataactc aaaaaagtaa gtgaaaaagc
     1321 aaccgcacct gcagttgctt ttatcgtttt cttattcttg atagcgtgtt ggtcctgcac
     1381 ttgcactacg gatattcaag cgtttcttga gatagaagcg aagggctagg agggctgctc
     1441 caataatagc tagtggcaat ggatccagta ctgggttaag gctagctggt aggaagcttg
     1501 ttgcaaagaa gacaagcaac caaaggaaca tagaagctag gataactagt acagatttcc
     1561 agaaaggtgg acgttgactg cgatccatat ctggtccata gtattggtaa acaaagtagt
     1621 acatcaagta gaaggcaaat ccaccaacca gtccaactaa tagaagagta atcaatccat
     1681 agccgaaagc ttgatctgcc gcaaagaagg ttgtgagggc gctgacaagg gcaaagaggc
     1741 tagtgatgaa aagagctgag tccataatca tcagttttgg gtcatcattt tcttttggat
     1801 gctctttttc gtactgctct ttgacagtga agctatgagc ccaatgagtt ggtgcgccgt
     1861 atagggaacg ggcagttgta cctttagatt gctcctcaag gatttgggga atgacttcct
     1921 caaaaatagt ctggatttca gcgtctgttt tcccatcttt gatgaattgt tgggtagcaa
     1981 tgtggacaaa ctcttggttt ttcttagtta atttttgtag atcaatttga gacataggaa
     2041 ctcctcttag aaccattttt tatggatgag atagagagtg agcgagacac tcatagcaaa
     2101 ggcgataaag acgattaacc agaaggcatt tggctctccg tttaggggga tttcattatc
     2161 cttaaagttc atcccgtagg cagaaaagac catggttggg atggacatga cgatggtcac
     2221 aagggccaag gttttcatga tgttgttctg gttgttagaa atgatagagg caaaggtctc
     2281 tgtcatagaa tgcaagacgt ttccataaat atctgccatc tcgatggcct gttgggtttc
     2341 aatcagggtg tcttcaagca ggtcttcgtc ctcaaggtat ttcttgatat tgctggttga
     2401 actggtcaat ttcttaatca cgcgctcatt tgttttgagg gaggccttga aatagacgat
     2461 agttttttcc aattccatga gctcaatcaa ttcttcatta cgagttgatt gatgcagttg
     2521 actttcgatt tgttcactct tgcggtcgat tgaacgaagg gctgttaggt aaagctctgc
     2581 attgcgataa agaatttgaa agataaaacg tgaacgcatg aaggtataga aattacgcaa
     2641 tcgacggttg ataaagacat caaggacagg tagtggttcc aaacacgtag tgataatggt
     2701 ttcctcagtg atgataatac caagcgggat ggttacgtag taggtgcggt tatttctttc
     2761 ctccgtgacc ggcacgtcta cgataatcag ggtatactcg tcttcaatgg taatacgaga
     2821 catttcttcc gcatcgagcg gtgctcgaag gtcggcaata tcaatatcga aggtgttggc
     2881 gatttcgagt gattcatttt gagtcggatt gacgagattg atccaagtac ccggttcaag
     2941 cgtatcgatc tctttaaatt cagttgttgt agagagaaaa acttgtttca tatcccttat
     3001 cctttctcat ttttcagatt ttttcacact gtactattat actacaaaat cggccttttg
     3061 ggtaatagaa tctcactttt tttggtataa tggtaagcaa taatggacta gaaagaacaa
     3121 agatgcaaga taaaattgtc attcatgggg cgcgtgccca taatttaaaa aatattgatg
     3181 tggagattcc gcgagacaag ttggttgtcg tgactggctt gtcaggttca gggaagtcca
     3241 gtctggcttt tgataccctc tatgcggagg gacaacgtcg ctatgtagag agtttgtccg
     3301 cctacgctcg tcagttcttg ggaaatatgg aaaaaccaga tgtagatgcc attgatggtc
     3361 tcagcccagc tatttccatc gaccagaaaa cgactagtaa aaatcctcgc tcgacggtgg
     3421 gaactacgac tgaaatcaat gactatctgc gtctcctcta cgcacgtgtg gggacgcctt
     3481 actgtatcaa cggacatgga gctatcaatg cttcttcggt ggagcaaatc gttgataagg
     3541 ttttggagtt acctgaacgc cagcgcttgc agatcttggc tcctgtcatc cgcaagaaaa
     3601 aaggccaaca taagagtgtt atcgagaagg ttcagaaaga cgggtatgtt cgtgttcgtg
     3661 tggatgggga agtctatgat gtgaccgaag tgccagagtt gtctaagagc aagcaacaca
     3721 atatcgatgt cgtggttgac cgtattgtta tcaaggaggg cattcgtagt cgtctctttg
     3781 attccattga ggctgccctt cgtattgcag aaggttatgt cattatcgac accatggacg
     3841 attcggagtt gctcttctct gagcattatg cctgtccagt ttgtggtttt actgtcccag
     3901 agttagaacc tcgtctcttc tctttcaatg ctccttttgg ctcttgtagt gagtgtgacg
     3961 gcttggggat caagctggag gtagatactg atttggtagt gccagatgcc agcaaaacct
     4021 tacgtgaggg agcgctagca ccttggaatc ctatctcatc caactactat ccaaacatgt
     4081 tagagcaggc catgaaagtc tttggagtgg ctatggataa gccttttgag gacctgtcag
     4141 aagaagataa gaacttgatt ctctatgggt cagatggtaa ggaattccat ttccactatg
     4201 agaatgaatt tggtggcgtg cgcgatatcg acattccgtt tgagggagtt atcaataata
     4261 tcaaacgtcg ttaccatgaa accaatagcg attacactcg cactcagatg cgtctctaca
     4321 tgaatgagct gacctgcgga acctgtcaag gctatcgtct caatgaccag gccttgtctg
     4381 tccgtgtggg cggccagcaa gggccacata ttggagaaat ctcagacctg tctatcgctg
     4441 accacttgga cttggtgagc cagttaactt tgtctgaaaa tgaagccatc attgctcgtc
     4501 ccattctcaa ggaaatcaag gatcgtttga ccttccttaa taatgtgggt cttaactatc
     4561 taaccctgtc acgttcagca ggaacccttt caggtgggga aagtcagcgc attcgtttgg
     4621 caacccagat tggttccaac ctatcaggtg tcctctatat cctagacgag ccgtcaatcg
     4681 gtcttcacca gagggacaat gaccgcctga ttgccagtct gaaaaagatg cgtgacttgg
     4741 gcaatactct tatcgtggtg gaacacgacg aagataccat gcgtgaagct gattatctga
     4801 ttgacgttgg tcctggtgcc ggtgtttttg gtggtgagat tgttgcagca ggtacaccta
     4861 agcaagtggc tcgcaacagc aagtctatca caggtcagta cttgtcaggc aaacgtgtca
     4921 tcccagtacc agaagagcgc cgtgtcggaa atggtcgttt tattgaagtg acaggagcgc
     4981 gtgagaacaa cttgcaaaat gtcacggctc gctttccact aggaaaattt atcgcagtga
     5041 caggtgtgtc gggttcaggg aaatcgactc taatcaacag cattctcaaa aaagccatta
     5101 cccagaagct caaccgtaat tcagacaaac ctggtaagtt taagactatt acagggattg
     5161 agcatgtcga ccgtttgatt gatattgacc agagccctat cggacggacg ccgaggtcta
     5221 acccagctac ctatacagga gtttttgacg atatacgtga cctttttgct cagacaaatg
     5281 aagccaagat tcgaggctac aagaaggggc gcttcagttt caacgtcaag ggaggtcgct
     5341 gtgaagcttg ctcaggtgac gggattatca agattgagat gcacttcttg ccagatgttt
     5401 atgtggcttg tgaagtttgc cacggaaccc gctataacag tgaaacccta gaagttcact
     5461 acaaggaaaa gaatatttcg caggtcttgg atatgacggt caacgatgcg gtagaatttt
     5521 tccaacacat tccgaaaatt caacgcaaac tccagaccat caaggatgta ggtctaggct
     5581 atgtgacctt gggacagcca gctaccaccc tttctggggg agaagcccaa cgtatgaagt
     5641 tagctagcga actccacaaa cgctcgacag gaaaatcttt ctacattctg gatgagccga
     5701 cgacagggct tcatacagag gacattgctc gcctgctcaa ggttttagct cgctttgtag
     5761 acgacggcaa tacagtcctc gtcatcgagc acaatctgga tgttatcaag acggcagacc
     5821 atatcattga cttgggacct gagggcggtg tcggtggtgg aaccatcatc gtaacaggaa
     5881 ctccagaaga agtagctgcc aacgaagcca gctatacagg acactatttg aaaggaaagt
     5941 tacatcatga ataaacgcgt acaagcattt ctagctaaaa tgcaagaaaa agaactagat
     6001 ggtatcatca tcaacaatct taaaaacgtc tattatttga ctggtttttg gggctcaaac
     6061 ggaacagtct ttatcagccg tgaccgtcag gtcttagtga cagactctcg ctatatcatc
     6121 gcagctaagc aagaaaccag tggttttgag attgtggctg atcgtgatga attggctgtc
     6181 attgcaggaa ttgttaagga catgaacttg actcgtatcg gttttgaaga tgagatttca
     6241 gtgtcttatt accaccgtat gcaggcagct tttgcaggtt tgaacttgct tacacaaact
     6301 cagtttgtgg aaggtcttcg tatgattaag gatgaggcag agattgcagc gattcgcaag
     6361 gcttgttcta tctcagacca agctttccgc gatgcgcttg actttatcaa accaggaaaa
     6421 actgaaattg agattgccaa cttccttgac ttccgcatgc gtgagttggg agcatctggc
     6481 ttatcttttg atacgatcct agctagcggt atcaattctt ctaaacccca tgcccatcca
     6541 atgcacaaac cagtggagtt gggagaagcc attaccatgg acttcggctg tctctatgac
     6601 cactatgtca gtgatatgac ccggactatc tatctagggc atgttagcga tgagcaggca
     6661 gagatttaca atacggttct aaaagctaac caagccttga ttgaccaagc taaggcaggc
     6721 ttaggtttcc gtgactttga caaaatccct cgtgatatta tcattgaggc aggttatggc
     6781 gactacttta ctcacggcat tggccacggt attggtctgg atatccatga ggaaccctac
     6841 tttagccaga cttctacaga aactattaag acaggtatgg ccttgaccga tgaaccaggt
     6901 atctatatcg aaggcaaata tggcgttcgt atcgaggatg atatcctgat tacagagaca
     6961 ggttgtgaat tattgaccct agctccaaaa gagttgatag tcatttagtt gtttgtcaag
     7021 aaaaaagtac gaaaatgacg aaaaaagtca aaaaaaatta aaaataggtc gcaagtcgga
     7081 tgttttttat ggtataatag actaaactga tagtaacatg tagcgaaagg ggtaggtaca
     7141 tgattaaaat ttatacagtc tcaagttgta ctagctgtaa aaaagcaaaa acctggctca
     7201 atgcccacca gttaagttat aaagaacaaa accttggtaa agaaggaatt acgagagaag
     7261 aattactgga tattctaacc aaaacagata acggaatagc cagcatcgtt tcgtctaaaa
     7321 atcgctatgc caaagccctt ggagtggata ttgaagattt gagtgtcaat gaagttctca
     7381 atctgattat ggaaacaccg agaattttaa agagcccaat ccttgtagat gaaaaacgcc
     7441 tgcaagttgg ctacaaggaa gacgatattc gtgccttcct accacgctct gtccgtaatg
     7501 tagaaaatgc agaagcacgt ttacgtgcag ctctataaac atcaaggctg ggagcaattc
     7561 ccagtcttat tctattttaa tttcaaaaag aaagaagaaa gaaatgaaaa aaatagttct
     7621 tgttagtcta gctttccttt ttgtcctggt tggttgcgga cagaaaaaag aaactggacc
     7681 agctacaaaa acagaaaagg atacgcttca gtcggcattg ccagttattg aaaatgccga
     7741 gaagaataca gttgtaacca agactttggt cttgcccaag tcagatgatg gtagccagca
     7801 aactcaaacc attacataca aggacaagac ttttttgagc ttaacgattc aacaaaaacg
     7861 tccagtttct gatgagttga agacttatat tgaccaacat ggagtggaag aaactcaaaa
     7921 agctcttctt gaagcggagg agaaggataa gtctatcatt gaagctcgta aattggtagg
     7981 tttcaaactt gaaacaaaac tattgagcgc aacggaactt caaacaacga ctagttttga
     8041 ttttcaagtt ctggatgtca agaaggcttc ccagttggaa catctgaaga atattggttt
     8101 ggaaaatctt ttgaaaaatg aaccaagcaa atatatttca gacagattgg caaatggtgc
     8161 gacagaacag tagaaaatcc aaataaatag actgcctgaa ttgtagaagg taaggaatta
     8221 tgctataatg gtactattct aaggaaagag ggtgttagaa tgggatttac tgaagaaaca
     8281 gtacgtttta aattggacga ttccaataaa aaagaaatta gcgaaacttt gacagatgtt
     8341 tatgcttcgt tgaacgataa gggttacaac ccaattaacc aaatcgtagg ttacgtattg
     8401 agtggagacc ctgcctacgt tcctcgttat aataatgcac gaaatcaaat ccgtaagtat
     8461 gagcgtgatg aaatcgttga ggaattggtt cgctactacc tcaaaggaca aggagtcgat
     8521 ctataaccta tgagaattat gggattggac gtcggttcaa aaacggtagg ggtggcgatt
     8581 agcgatccgc ttggttttac agctcaaggg cttgaaatca tccagataaa tgaagaacaa
     8641 ggccaatttg gttttgaccg cgttaaggaa ttggttgata cttacaaggt ggaacgattt
     8701 gtagtgggct tgcctaaaaa catgaacaat acaagtggac cgcgcgtaga agctagtcaa
     8761 gcctacggag caaagctaga agagtttttt ggtttaccag tagactatca ggatgaacgc
     8821 ttgacaacag tggctgctga gcgcatgttg attgaacaag cagatatcag tcgcaataag
     8881 cgcaagaaag tcattgataa gttagcagct cagctgattt tacaaaatta tttagataga
     8941 aaattttaat ataaaggaga ggctatgtca cacgatcata accacgacca cgaagaacgt
     9001 gaactaatca cactagtaga tgagcaagga aatgaaacct tgtttgaaat ccttttgaca
     9061 attgatggaa aagaagaatt tggtaaaaac tatgttcttc tagtaccagt taacgcagaa
     9121 gaagacgaag acggacaagt tgaaatccaa gcttactcat tcattgaaaa tgaagatgga
     9181 acagaaggcg aattgcaacc aatcccagaa gactcagaag acgaatggaa catgattgaa
     9241 gaagtcttca acagctttat ggaggagtaa aaacgtccgg gggacgtttt tagccctgcg
     9301 ctagaaatta gaaacgcagg tagtctggga gactgtatca gcccaactcc agaaatgggg
     9361 aagagttgga acgtccagtg aacattttta ccccatattc acattcatag tggctagtta
     9421 ttagctaacc aggtaagagg tcgggacgaa aatcctggcc ttgtttttat tagtaaccat
     9481 actttaatgc gataattgat tggacttttg tcaaggagat gtgtatgttt gaagtagaag
     9541 aatggctcca tagtcggatt ggtttgaatt ttcgatcagg tttgggccga atgcagcaag
     9601 cggtggattt gttaggaaat cctgagcagt cttaccctat tatccacgta acagggacta
     9661 atgggaaagg atctaccatt gcttttatga gggaattatt tatggggcat ggcaaaaaag
     9721 ttgcgacctt tacctcccct catatcgtct ctatcaatga ccgaatctgc attaatgggc
     9781 aacctatagc agacgcagac tttatccgtt tgactgatca ggtcaaggag atggagaaaa
     9841 cgcttctgca aactcctgac cagttgtcct tttttgaatt gctgaccttg gttgcttttc
     9901 tttattttag ggagcaggag gtggatttgg ttttattaga agtgggaatt ggtggcttac
     9961 ttgacacgac caatgtggta actggagagc ttgctgtcat cacctccatt gggcttgacc
    10021 atcaagaaac cttgggtgat agtctagaag caattgcaga gcagaaagct ggtattttca
    10081 aggctggtaa aaaggcagtg attgcgaaat tgcctccaga agctagactt gtctgtcaga
    10141 aaaaagccga atctttagct gttaaccttt atcaggcagg tcaagatttt ttaatgctga
    10201 atggtgattt ttcaagctct ttactaaatc tttcacagct gaacataggc ttagaaggag
    10261 tctatcagca ggagaatgca gccttggcgt tgcaaacttt tcttcttttt atgggagaag
    10321 gaaaggaagc tgttgatgaa caggctgtaa gaaaggcctt ggaacagacc cattgggctg
    10381 gtcgcttgga gcgtattcgc ccacagattt acttggatgg tgctcataac ctccctgcct
    10441 tgactcgctt ggttgagttt atcaaagaaa aagagcagga aggctatcga cctcaaatcc
    10501 tctttggatc cttgaaacgt aaggattatc aagggatgtt gggttatctg actgaaaatt
    10561 tacctcaagt ggaactcaaa atgaccggct ttgactatca agggtctttg gacgagacag
    10621 atgtaacagg ttaccatgta attccctctt accgagaatt tatcagcagt ttcgaagaaa
    10681 gggcagacgc tcaagacttg ctgttcgtta cagggtctct ctattttatc tcagaagtac
    10741 ggggctacct gctggaccgt gagcagataa attgaccccc ttgtttgaac ttgttatact
    10801 aggggaattg ctggttagag aaaatttctc taaattggta gcagaaagga aattcatcat
    10861 gaaattaaaa agattcacac tttctcttgc ttctctagca agttttagtc tcttagtagc
    10921 ttgttcacaa agagctcaac aggttcaaca gcctgttgct cagcaggtcc aacagcctgc
    10981 tcaacagaat accaatactg caaatgcagg aggtaaccaa aatcaagcgg ctccagtaca
    11041 aaaccaacct gttgctcaac cgaccgatat tgatgggact tatactggtc aggatgacgg
    11101 agaccgtatc actttagtgg taactggaac gactggtaca tggactgagc tcgaatctga
    11161 cggggatcag aaagtcaaac aggttacatt tgattcagca aatcaacgca tgattattgg
    11221 cgatgatgtc aaaatttaca ctgtaaacgg taatcaaatc gtcgtagatg atatggatag
    11281 agacccatcg gaccaaatcg ttttaactaa ataagactat gcaagtagaa agggctggat
    11341 ttttcagcct ttttactttt acctagaaaa taatcataaa tacttgcctt ctaccgaata
    11401 cctgcgttat actagtatca attacttgtt tttagcattc aaactatcaa ggaggggata
    11461 tgaaatatag gaaatttcaa ttattgatgt ccaagtatgg ctttagtctt tcgattatgc
    11521 tacttgaact ttgtcttgtt tttggtctct ttctttattt aggacgcatg gctcccattt
    11581 tatggattac tgtcctcatt ctactgagta tcatcacaat catttcgata gtcaaccgta
    11641 atacgactcc tgagaataag gtaacctggt tgttagtagc ctttgtgcca gtatttggtc
    11701 ccttgctcta tctgatgttt ggtgaaaggc gattgtccaa aaaagaaatc aaacaactga
    11761 agaagctagg ctctatgcat ttccaagaag caaatagcca gctactaaaa gagaaattaa
    11821 aagaaagtga caaggcagct tatggagtca tcaagtcctt attgagtatg gataccaatg
    11881 ctgacatcta tgatcaaact gcctctacat tttttcctaa cggagaagct atgtggaaaa
    11941 agatggtaga agatcttaaa aaggctgaga aatttatttt cttggaatat tacattatag
    12001 aagaaggttt gatgtggaat cgcatactag atatactaga gcaaaaggta gctcagggtg
    12061 tagaggttaa gatgctctat gatgatatcg gctgtatggc tactttaaca ggagattatg
    12121 cacatcgact tcgtcagctg ggcatcgagg cccataaatt caataaagtt attcctcgtt
    12181 tgacagtggc ttataataac agagatcata gaaaaatatt gattgttgat ggtcagatag
    12241 cctatactgg tggggtcaat ctggcagatg agtacattaa ccacgtcgag agatttggtt
    12301 attggaagga tagtggaatt cgcttagacg gactagcagt aaaagccctg acacgcttat
    12361 ttttgaccac ttggtacatt aatcgaggag aaattagtga ttttgatcaa tatcatttag
    12421 aaaatcattc tatcccgagt gacggtttaa ccattccata cggaagtgga cccaagccaa
    12481 tttttcgagc gcaggtaggg aaaaaagttt atcagagttt aatcaatcaa gcaacggaat
    12541 cggtctatat tacgacacct tatttgatta tagattatga tttaacagag acaatcaaaa
    12601 atgcagctat gagaggggtc gatgttcgaa ttatcacccc ttacatacca gataagaagt
    12661 tcattcagtt agtcacgaga ggagcttatc ccgaccttct ttctgctggt gttcggattt
    12721 atgagtatag tccaggtttt attcatagta agcagatgtt ggtagacgaa gattttgcgg
    12781 tggtggggac aatcaatctc gactaccgga gcttggtaca ccattatgaa aatgcagtct
    12841 tactctataa aactccttct ataatggaaa tcgcccgaga ttttcgaaat atatttgcag
    12901 attctcagga agtctatcct cattctatca aaacgagctg gtatcaaaag cttgtaaaag
    12961 aaatcgccca gctattcgcc cctatcttat aagaaatcta gaacttagga tacaaaccag
    13021 acctattttt cttgtctttt acgaatagaa agaaagagga ggggaattca tgctaactaa
    13081 ggaagaagtg aatgatatga ttgagttcaa acagacacat ttacaccgct ttcgggaaat
    13141 tgaaaccttt gtgaaactat gcaaaaaatc actcaaacag ccatcgcaag ctgacaatcc
    13201 taggactttt tgatatacta agacagataa attgaacagg agaaacgata tgagtgtgta
    13261 tggtagagta gaagaagttc ataaggagaa tcgtgaacct ctagaatacc aaatcgaaca
    13321 agaatcgcat catcgtgaat caagtcgtct tcctttggtg aaaattttac tatggagtac
    13381 gcttgtaacg gggataactc taggagtacc gctattactc gacttaatga gtgcacaaga
    13441 agtgcaggat ttttatgcag gttgggcgct tcatcagaca gggaagattt acagcgacta
    13501 ttatggaagt caaggtttgc tttattattt gctgacttac gtgagtcagg gcggatattt
    13561 ctttgccatc tttgagtggt tagccttggt agcaggagga tttttccttt ttagatcagc
    13621 ggacaccttg acagagcaag gagaccaagc tggacatctg gtgactattt tttacatgct
    13681 agttacaggt cttgcttttg gtggaggcta tgcgactctt ttagcgcttc ctttcttatt
    13741 cgcagccttt agtttagttg cggcttacct aagcaatcca agccatgata agggatttgt
    13801 acggattggg ctagctttgg caggcggatt tttctttgct cccttatcat cgctcctgtt
    13861 tattgctgta gtgagtttag gcttgttggt ctttaacctt gggcatagac gttttgtgca
    13921 tgggttttat cagtttcttg cagtggcttt aggtttttca cttgtctttt atccaactgc
    13981 ctactatagt gctgcaacag gaagttttgg ggatgcgatt agtggtattc gttatcctat
    14041 tgacagtatt cgctttgatt ttacttctaa aattttagag aatatgtttt tttatggctt
    14101 gttgtccctt ggtttgggat ttgtggtttg tatcttttta ggtctctttc aatccaaacc
    14161 ctttaaacta tacgtcattt cggttcctgc aagtcttgtg gtaattttag gtttgatttt
    14221 acttttcttt tcacaagagc ctctgcacgc ttcttatttg atggtcgtct tccctgtttt
    14281 cctactttta ttggtaacca atattaagag tcaacagagg gggcgtggtg ttagaagaag
    14341 ccgaagagaa acgccagtta gcctatggag tcgtttcttc aaaggaaatc tatatctgct
    14401 agtttttggg cttgtctatc ttttgtctgt tccttttttg atgaagtttg tcctttatcc
    14461 ggtaccttat caagaacgta atcgtcttgc tgatttggta aaagaggaga caaatacgga
    14521 agatactatc tatgcatggg atgatactgc gactctttat cgtaagagtg agcgcttgtc
    14581 gccatcggcg attttgtccc cgttgcacta tacagcaact gaggaaaatc gtaataagtt
    14641 acttaatgac ttgaaagaaa aacaacctaa ggtgattgtg gtaaatgaca aggtggtagt
    14701 ctggtctgaa gtggaaacac tcttaaaaga aaattaccaa caagtaaaga ctgattactc
    14761 agagtttaaa gtctataaaa ttaaataacc aaatcaatat cttgtgtatt tttaaaaatt
    14821 ttaggatttt taacacaaga tattgatttt tctttttaga gtggtataat actttttaga
    14881 aagaacattt tagaaaagag catgcatatg attgcactag aagaaaaaat tacaattttg
    14941 ccaactctct tcgtcgagaa acgagatggg agacgtgttg tatttgatgt ggacaagatt
    15001 gacaaggctc tccacaaggc ggctgacaag gttatggatg tgacacccct ggttgaaaaa
    15061 cgcctcaatg atctgactga gcgaattatt acagaaattc atagtcgctt tccacaggga
    15121 attaagattt acgaaattca aaatatcgta gaacatgaac tccttgaagc caaagaatat
    15181 gcgctggctg aggagtatat tacttatcgg acacagaggg attttgagcg ctcaaaagcg
    15241 acggatatca actttagtat tcataaactt ctcaacaaag accagacagt tgtcaatgaa
    15301 aatgctaata aagacagtga tgtctttaac actcagcgtg atttgacagc agggattgtt
    15361 gggaaatcaa tcggactgca aatgcttcct aagcacgtag ccaatgccca ccaaaagggg
    15421 gatatccact atcacgattt ggactacagt ccctataccc ctatgaccaa ctgctgtttg
    15481 attgatttta agggtatgtt ggaaaatggt tttaagattg gaaatgcaga ggtagagagt
    15541 cccaagtcta tccagactgc gacagcacag atttctcaaa tcattgccaa cgttgcttct
    15601 agccagtacg gtggctgttc agctgaccgt atcgatgaaa ttttggcgcc ttatgcagag
    15661 aagaattatc aaaaacatct caaagatgca gaagagtggg tattgcctga aaaacgggaa
    15721 gattacgctt ggaagaaaac gcaaaaggac atctacgatg ccatgcaatc tcttgagtat
    15781 gaaatcaata ctctcttcac ttcaaatgga caaacacctt ttacttcgtt aggttttggt
    15841 ctgggaacca gtcgttttga acgagaaatt caaaaagcta ttttaaacat tcgcatcaag
    15901 ggtcttggtt cagaacaccg tacggctatc tttcctaaac ttattttcac tctgaaaaga
    15961 ggccttaatt tagaggaagg aactcccaac tacgacatca agcagttggc tcttgagtgt
    16021 gcaaccaaac ggatgtatcc agatgtttta tcttacgata agattgttga cttgacagga
    16081 tccttcaagg tgcctatggg ttgccgttct ttcctccaag gatggaagga tgaaaatggt
    16141 gtagaagtca attcaggtcg catgaatctg ggtgttgtga cggttaatct gcctcgtatt
    16201 gctcttgagt ctgaaggtga tatgaataag ttctgggaaa tcttcaacga gcgaatgaat
    16261 atcgcagaag atgctcttgt ttaccgtgtc gaacgcacta aagaggcgac accagcaaat
    16321 gctcctattc tttatcagta cggtgctttt ggccatcgtc taggtaaaga agaaagtgtt
    16381 gaccagctct ttaagaatcg tcgtgctacc gtttcgctgg gctatatcgg cttgtatgaa
    16441 gtagcgacag ttttctttgg taacagctgg gaaagtaatc cagatgctaa ggaattcacg
    16501 ctagacatca ttcacgatat gaaacgccgt gtagaagagt ggtcagacca atatggctac
    16561 catttctcta tctactcaac accatccgaa agtctgacag accgtttctg ccgactagat
    16621 atagacaagt ttggctctat tcctgatatc acagacaagg aatactacac caactctttc
    16681 cactacgatg ttcgtaaaaa tccaacaccg tttgaaaaat tggactttga gaaagtctat
    16741 ccagaagcag gtgcgtcagg tggtttcatc cattattgtg agtatccagt ccttcagcaa
    16801 aatccaaagg ccttggaagc tgtctgggat tatgcttatg accgtgtagg ctatctaggc
    16861 accaatactc cgattgaccg ttgctacaag tgtgactttg aaggggattt tgaaccaact
    16921 gagagagggt ttgcttgtcc aaactgtggc aatagcgacc ctaaaacagt agatgtggtg
    16981 aagcgaactt gtggctacct aggtaatcct caagcaagac cgatggtcaa cgggcgtcac
    17041 aaggaaatcg ctgcgcgtgt caaacatatg aatggttcaa cgattaaaat agctgggcat
    17101 caagtaacaa attagaaaga aatgaaatgg gaaaatatca actagacgat aaggggcgcg
    17161 cacaagtgac ccgttatcac gagaaacact ctaaaggtgg agctggtaag aaagaacgct
    17221 tgcttagctt cagagaacaa tttttaaaca agaacaagaa aaaataaaag tgagagccag
    17281 ctctcgcttt tctcatagtg ggaggtaagg atggaattac gcagaccaag attagcggat
    17341 aagaaagctg ttttagatat gatgacagag tttgaaaaga gtcaatcagc ccatgatgga
    17401 ggattttggg atacagagaa ttttgtgtat gaagagtggt tggaaagcaa tcaggaacag
    17461 gaaatgggga ttaatctgcc tgaaggatgg gttcctgcaa ttcagttagt ggctttttct
    17521 gagaaaggtc aagcagttgg atttcttaat ctccggttgc gcctcagtaa ctttttacta
    17581 gaagaaggtg ggcacattgg ctactccatc cgtccatctg aaagaggcaa gggttatgca
    17641 aaagagactc tccgtcaggg cttgcaagtt gctaaggaaa agaacatcaa gaaagctctg
    17701 gtgacctgta gcgtgaataa tcctgctagc agagcagtca ttctagcaaa tggtggaata
    17761 tttgaggatg ctcacaatgg agtcgagcgt tattggatag aggtagcgaa tgaataatcc
    17821 aaaaccacaa gaatggaaaa gcgaggaact tagtcaaggt cgtatcattg actacaaggc
    17881 ctttaacttt gttgatggcg aaggcgtgcg taactctctc tatgtatcag gctgtatgtt
    17941 tcactgcgag ggatgttata atgttgcgac ttggtctttt aatgctggca ttccctatac
    18001 agcagaatta gaagagcaga tcatggcaga ccttgcccaa ccctatgttc aaggcttgac
    18061 tttgctggga ggggaacctt ttctcaatac tggcattctc ttgcctctag ttaaacgcat
    18121 ccgaaaggaa ttgccagaca aggacatttg gtcctggacg ggctacactt gggaagaaat
    18181 gatgttggaa actccagata aactggaact cttatcattg attgacattc ttgtcgatgg
    18241 aagatatgat cgaactaaga gaaatctcat gctccagttt cgaggttcgt ccaaccaacg
    18301 aattatcgat gtgcaaaaat cgctcaaaag tgggcaagta gtgatttggg acaagctcaa
    18361 tgacggaaaa gaaagctatg aacaggtgaa gagagaatga agaaaaaaga cttagtagac
    18421 caactagtct cagagatcga gacggggaaa gtcaggacac tgggaatata cggtcatgga
    18481 gcttcaggta aatcaacctt tgcacaggaa ttgtaccaag ctttagattc tactacagta
    18541 aatttgctag agacagatcc ttatatcacc tcaggacgcc atctggtagt acccaaggac
    18601 gcgccgaatc aaaaggtgac agccagtctg ccagtggcgc atgaactgga gagtttgcag
    18661 agagatatcc ttgccttgca ggcgggtatg gatgtcttga caattgaaga accttggaag
    18721 gctagtgagg tcttgtctgg agccaaacca attttgattg tcgaagggat gtctgttggc
    18781 tttctaccca aggaactctt tgaaaaaacc atctgtttct acacggatga ggagaccgaa
    18841 ttaaagcgac gccttgctag agatacgact gtgagaaatc gcgatgcatc ctttatattg
    18901 gctagccatc agatgagacg ggagcagtat ctgcgatact ataaagaaac tgagtctaag
    18961 gcggatatct tagtggacta atcagaagat aaatttgatg tcaagaggac ttaacttatg
    19021 tatagaagaa atatagaaga aaatccctaa ttttagaaag aaaaagtagg aaaacagttt
    19081 catcccaaaa aaacgaaaaa acagcaacaa atcccttgca atcgcagggg ctttgtgtta
    19141 ttctattatg gtgctgtaaa ttacagcctt agttttgatg caagaggttg cgacacgctc
    19201 ggttgcattg ccacgcaaca ccgcgtcggt tttcttgtgg agctagccta ttatcttaaa
    19261 tagacgaaaa ggagaaaaag atggcaaaca aaaaaatccg tatccgtttg aaagcttacg
    19321 aacaccgtac gcttgacaca gcggctgcaa aaatcgtaga atcagctact cgtacaggtg
    19381 cacaagttgc gggtccaatc ccacttccaa ctgaacgtag cctctacaca atcattcgtg
    19441 cgactcacaa atacaaagac tctcgcgaac aatttgaaat gcgtacacac aaacgtttga
    19501 tcgatatcgt taacccaact caaaaaacag ttgatgcctt gatgaaattg gatcttccaa
    19561 gtggtgtaaa cgtagaaatc aaactttaat ctaaaatata aaagagcaga ggctggtgtt
    19621 tcaatctaat tgaacacggg ctaaactcgg tgtgaaaaag ataaacttcc tagtgtctgc
    19681 tagacactgc gtcagtttcc tattttcacg ttgagtttga cgccctttgt atcttagact
    19741 tgagcataaa aaacgctcgt taaaaacttt ttgaataaaa aatatagaaa aggaactatt
    19801 ttctcatgac aaaaggaatc ttagggaaaa aagtgggaat gactcaaatc ttcactgaag
    19861 ctggcgaatt gatccctgta acagttattg aagcaactcc aaacgttgtt cttcaagtta
    19921 aaactgttga aacagacgga tacaacgcta tccaagttgg tttcgatgac aaacgcgaag
    19981 tattgagcaa caaacctgct aaaggacatg tagcgaaagc taacacggct cctaagcgct
    20041 tcattcgtga attcaaaaac gttgaaggct tggaagttgg tgctgaaatt acagttgaaa
    20101 cattcgcagc tggagacgtt gttgacgtaa ctggtacttc taaaggtaaa ggtttccaag
    20161 gtgttatcaa acgccacgga caatcacgtg gaccaatggc tcacggttct cgttaccacc
    20221 gtcgtccagg ttctatggga cctgttgcac ctaaccgcgt attcaaaggt aaaaaccttg
    20281 cagggcgtat gggtggcgac cgcgtaacaa ttcaaaacct tgaagttgta caagttgttc
    20341 cagaaaagaa cgttatcctt atcaaaggta acgtaccagg tgctaagaaa tctcttatca
    20401 ctatcaaatc agcagttaaa gctggtaaat aataaagaaa ggggaaatca gtcacaatgg
    20461 caaacgtaac attatttgac caaactggta aagaagctgg ccaagttgtt cttagcgatg
    20521 cagtatttgg tatcgaacca aatgaatcag ttgtgtttga tgtaatcatc agccaacgcg
    20581 caagccttcg tcaaggaaca cacgctgtta aaaaccgctc tgcagtatca ggtggtggac
    20641 gcaaaccatg gcgtcaaaaa ggaactggac gtgctcgtca aggttctatc cgctcaccac
    20701 aatggcgtgg tggtggtgtt gtcttcggac caactccacg ttcatacggc tacaaacttc
    20761 cacaaaaagt tcgtcgccta gctcttaaat cagtttactc tgaaaaagtt gctgaaaaca
    20821 aattcgtagc tgtagacgct ctttcattta cagctccaaa aactgctgaa tttgcaaaag
    20881 ttcttgcagc attgagcatc gattctaaag ttcttgttat ccttgaagaa ggaaatgaat
    20941 tcgcagctct ttcagctcgt aaccttccaa acgtgaaagt tgcaactgct acaactgcaa
    21001 gtgttcttga catcgcaaat agcgacaaac ttcttgtcac acaagcagct atctctaaaa
    21061 tcgaggaggt tcttgcataa tgaatttgta tgatgttatc aaaaaacctg tcatcactga
    21121 aagctcaatg gctcaacttg aagcagggaa atatgtattt gaagttgaca ctcgtgcaca
    21181 caaacttttg atcaagcaag ctgttgaagc tgctttcgaa ggtgttaaag ttgccaatgt
    21241 taacacaatc aacgtaaaac caaaagctaa acgtgttgga cgttacactg gttttactaa
    21301 caaaactaaa aaagctatca tcacacttac agctgattct aaagcaatcg agttgtttgc
    21361 tgctgaagct gaataatcta aggaggaaat atcgtgggaa ttcgtgttta taaaccaaca
    21421 acaaacggtc gccgtaatat gacttctttg gatttcgctg aaatcacaac aagcactcct
    21481 gaaaaatcat tgcttgttgc attgaagagc aaggctggtc gtaacaacaa cggtcgtatc
    21541 acagttcgtc accaaggtgg tggacacaaa cgtttctacc gtttggttga cttcaaacgt
    21601 aataaagaca acgttgaagc agttgttaaa acaatcgagt acgatccaaa ccgttctgca
    21661 aacatcgctc ttgtacacta cactgacggt gtgaaagcat acatcatcgc tccaaaaggt
    21721 cttgaagtag gtcaacgtat cgtttcaggt ccagaagcag atatcaaagt cggaaacgct
    21781 cttccacttg ctaacatccc agttggtact ttgattcaca acatcgagtt gaaaccaggt
    21841 cgtggtggtg aattggtacg tgctgctggt gcatctgctc aagtattggg ttctgaaggt
    21901 aaatatgttc ttgttcgtct tcaatcaggt gaagttcgta tgattcttgg aacttgccgt
    21961 gctacagttg gtgttgtcgg aaacgaacaa catggacttg taaaccttgg taaagcagga
    22021 cgtagccgtt ggaaaggtat ccgcccaaca gttcgtggtt ctgtaatgaa ccctaacgat
    22081 cacccacacg gtggtggtga aggtaaagca ccagttggtc gtaaagcacc atctactcca
    22141 tggggcaaac ctgctcttgg tcttaaaact cgtaacaaga aagcgaaatc tgacaaactt
    22201 atcgttcgtc gtcgcaacga gaaataatat taaactagtc gcttaagcaa ctagtaaatc
    22261 cgccagctcg gtagcgctcc ataggagtgc aagccgctgt ggtacaacat ttaaaggaga
    22321 aaatataaaa atgggacgca gtcttaaaaa aggacctttc gtcgatgagc atttgatgaa
    22381 aaaagttgaa gctcaagcta acgacgaaaa gaaaaaagtt attaaaactt ggtcacgtcg
    22441 atcaacgatc ttcccaagtt tcattggtta cactattgca gtttatgacg gacgtaaaca
    22501 cgtacctgtt tacatccaag aagacatggt aggccacaaa cttggtgaat ttgcaccaac
    22561 tcgtacttac aaaggtcacg ctgcagacga caagaaaaca cgtagaaaat aaggagaaca
    22621 taaatggcag aaattacttc agctaaagca atggctcgta cagtacgtgt ttcacctcgt
    22681 aaatcacgtc ttgttcttga taacatccgt ggtaaaagcg tagccgatgc aatcgcaatc
    22741 ttgacattca ctccaaacaa agctgctgaa atcatcttga aagttttgaa ctcagctgta
    22801 gctaacgctg aaaacaactt tggtttggat aaagctaact tggtagtatc tgaagcattc
    22861 gcaaacgaag gaccaactat gaaacgtttc cgtccacgtg cgaaaggttc agcttcacca
    22921 atcaacaaac gtacagctca catcactgta gctgttgcag aaaaataagg aggtaaaatc
    22981 gtgggtcaaa aagtacatcc aattggtatg cgtgtcggca tcatccgtga ttgggatgcc
    23041 aaatggtatg ctgaaaaaga atacgcggat taccttcatg aagatcttgc aatccgtaaa
    23101 ttcgttcaaa aagaacttgc tgacgcagca gtttcaacta ttgaaatcga acgcgcagta
    23161 aacaaagtta acgtttcact tcacactgct aaaccaggta tggttatcgg taaaggtggt
    23221 gctaacgttg atgcactccg tgcaaaactt aacaaattga ctggaaaaca agtacacatc
    23281 aacatcatcg aaatcaaaca acctgatttg gatgctcacc ttgtaggtga aggaattgct
    23341 cgtcaattgg agcaacgtgt tgctttccgt cgtgcacaaa aacaagcaat ccaacgtgca
    23401 atgcgtgctg gagctaaagg aatcaaaact caagtatcag gtcgtttgaa cggtgcagat
    23461 atcgcccgtg ctgaaggata ctctgaagga actgttccgc ttcacacact tcgtgcagat
    23521 atcgattacg cttgggaaga agcagatact acatacggta aacttggtgt taaagtatgg
    23581 atctaccgtg gtgaagttct tccagctcgt aaaaacacta aaggaggtaa ataaccaatg
    23641 ttagtaccta aacgtgttaa acaccgtcgt gagttccgtg gaaaaatgcg cggtgaagca
    23701 aaaggtggaa aagaagtagc attcggtgaa tacggtcttc aagctacaac tagccactgg
    23761 atcactaacc gccaaatcga agctgctcgt atcgccatga ctcgttacat gaaacgtggt
    23821 ggtaaagttt ggattaaaat cttcccacac aaatcataca ctgctaaagc tatcggtgtg
    23881 cgtatgggat ctggtaaagg ggcacctgaa ggttgggtag caccagttaa acgtggtaaa
    23941 gtgatgttcg aaatcgctgg tgtatctgaa gagattgcac gtgaagcgct tcgacttgct
    24001 agccacaaat tgccagttaa atgtaaattc gtaaaacgtg aagcagaata aggagaaggc
    24061 atgaaactta atgaagtaaa agaatttgtt aaagaacttc gtggtctttc tcaagaagaa
    24121 ctcgcgaagc gcgaaaacga attgaaaaaa gaattgtttg aacttcgttt ccaagctgct
    24181 actggtcaat tggaacaaac agctcgcttg aaagaagtta aaaaacaaat cgctcgtatc
    24241 aaaacagttc aatctgaagc gaaataatag actagggaag gagaaatttc aatggaacgc
    24301 aataatcgta aagttcttgt tggacgtgtt gtatctgaca aaatggacaa gacaatcaca
    24361 gttgtagttg aaacaaaacg taaccaccca gtctatggta aacgtattaa ctactctaaa
    24421 aaatacaaag ctcatgatga aaacaatgtt gccaaagaag gcgatatcgt acgtatcatg
    24481 gaaactcgcc cgctttcagc tacaaaacgt ttccgtcttg tagaagttgt tgaagaagcg
    24541 gtcatcatct aatcaaacct gaaaggagaa aactgaaatg attcaaacag aaactcgttt
    24601 gaaagtcgca gacaacagcg gtgctcgcga aatcttgact atcaaagttc ttggtggttc
    24661 aggacgtaaa tttgcaaaca tcggtgatgt tatcgtggca tctgtaaaac aagctactcc
    24721 tggtggtgcg gttaaaaaag gtgacgttgt taaagcagtt atcgttcgta ctaaatcagg
    24781 tgctcgtcgt gctgatggtt catacatcaa atttgacgaa aacgcagcag ttatcatccg
    24841 tgaagacaaa actcctcgcg gaacacgtat ctttggccca gttgcacgtg aattgcgtga
    24901 aggtggcttc atgaagatcg tgtcacttgc tccagaagta ctttaatttt taggaacaaa
    24961 ctagtcccct agcttcaagc tagggtgccc ttatgggcgt aagaaaaatc aaggagaaac
    25021 ctaatgtttg taaaaaaagg cgacaaagtt cgcgtaatcg ctggtaaaga taagggaaca
    25081 gaagctgttg tccttactgc ccttccaaaa gtaaacaaag ttatcgttga aggtgttaac
    25141 attgttaaga aacaccaacg tccaactaac gagcttcctc aaggtggtat catcgagaaa
    25201 gaagcagcta tccacgtatc aaacgttcaa gttttggaca aaaatggtgt agctggtcgt
    25261 gttggataca aatttgtaga cggtaaaaaa gttcgctaca acaaaaaatc aggcgaagtg
    25321 cttgattaat cacgaaggaa aggagaagta taatggcaaa tcgtttaaaa gaaaaatatc
    25381 ttaatgaagt agttcctgct ttgacagaac aattcaacta ctcatcagtg atggctgtgc
    25441 ctaaagtaga taagattgtt ttgaacatgg gtgttggtga agctgtatca aacgctaaaa
    25501 gccttgaaaa agctgctgaa gaattggcac ttatctcagg tcaaaaacca cttatcacta
    25561 aagctaaaaa atcaatcgcc ggcttccgtc ttcgtgaagg tgttgcgatc ggtgcaaaag
    25621 ttacccttcg tggtgaacgt atgtacgaat tcttggataa attggtatca gtttcacttc
    25681 cgcgtgtacg tgacttccac ggtgttccaa caaaatcatt tgatggacgc gggaactaca
    25741 cacttggtgt gaaagaacaa ttaatcttcc cagaaatcaa cttcgatgac gttgacaaaa
    25801 ctcgtggtct tgacatcgtt atcgtaacaa ctgctaacac tgacgaagag tcacgtgcat
    25861 tgcttacagg ccttggaatg ccttttgcaa aataatatag gaggtaaatc taatggctaa
    25921 aaaatcaatg gtagctagag aggctaaacg ccaaaaaatt gttgaccgtt atgctgaaaa
    25981 acgtgctgca ttaaaggcgg caggggacta cgaaggttta tctaaattac ctcgcaacgc
    26041 ctcaccgact cgtttacata atcgttgtag agttacgggg cgcccacatt cagtttaccg
    26101 caaatttggt ctgagtcgta tcgcttttcg cgaacttgcg cataaaggtc aaattcctgg
    26161 tgtaacaaaa gcatcttggt aatttaagat atcaagagtg tcaaaactcc aagtaaaaat
    26221 aggaaacttg acgaagaaac taaagtttct aggaaagttt atctttttca cacagagttt
    26281 agcccgggtt caattgggct tgccaatttg aacacgagct acagctttgg caaaaaagac
    26341 caatttgctt tggagcattg cttctgcatt aaattgtcta tttttgctcg tgctgttacg
    26401 ctctttgtat catgtattaa ctagcaagtg caacttgcaa actactagta agaggagaaa
    26461 aacaaaatgg ttatgactga cccaatcgca gacttcctaa ctcgtattcg taatgctaac
    26521 caagctaaac acgaagtact tgaagtacct gcatcaaaca tcaaaaaagg gattgctgaa
    26581 atccttaaac gcgaaggttt tgtaaaaaac gttgaaatca ttgaagatga caaacaaggc
    26641 gtcatccgtg tatttcttaa atacggacca aatggtgaga aagttatcac taacttgaaa
    26701 cgtgtttcta aaccaggact tcgtgtctac aaaaaacgtg aagaccttcc aaaagttctt
    26761 aacggacttg gaattgccat cctttcaact tctgaaggtt tgcttactga taaagaagca
    26821 cgccaaaaga atgttggtgg tgaggttatc gcttacgttt ggtaaaatca agatacaaag
    26881 ctcgtaaaga acaaagcaaa attaggaagt tggagaagtt tgtttacaaa caagccaact
    26941 tatctatttt gcacagttct tagagcgtgt tcagttcagc tcttgaacta aataagtatc
    27001 tgaaccccgt gaaaactggc cgttctggcc tgacaattta acaggagaaa ataaacatgt
    27061 cacgtattgg taataaagtt atcgtgttgc ctgctggtgt tgaactcgct aacaatgaca
    27121 acgttgtaac tgtaaaagga cctaaaggag aacttactcg tgagttctca aaagatattg
    27181 aaatccgtgt ggaaggtact gaaataactc ttcaccgtcc aaacgattca aaagaaatga
    27241 aaactatcca cggaactact cgtgcccttt tgaacaacat ggttgttggt gtatcagaag
    27301 gattcaagaa agaacttgaa atgcgtgggg ttggttaccg tgcacagctt caaggatcta
    27361 aacttgtttt ggctgttggt aaatctcatc cagacgaagt tgaagctcca gaaggaatta
    27421 cttttgaact tccaaaccca acaacaatcg ttgttagcgg aatttcaaaa gaagtagttg
    27481 gtcaaacagc tgcttacgta cgtagccttc gttcaccaga accatataaa ggtaaaggta
    27541 tccgttacgt tggtgaattc gttcgccgta aagaaggtaa aacaggtaaa taatgttgag
    27601 tggttgatca tcaaccacca acctattttc caactttgtg catagcacac gatttaaaac
    27661 taaagaggtg aaaactgtga tttcaaaacc agataaaaac aaactccgcc aaaaacgcca
    27721 ccgtcgcgtt cgcggaaaac tctctggaac tgctgatcgc ccacgtttga acgtattccg
    27781 ttctaataca ggcatctacg ctcaagtgat tgatgacgta gcgggtgtaa cgctcgcaag
    27841 tgcttcaact cttgataaag aagtttcaaa aggaactaaa actgaacaag ccgttgctgt
    27901 cggtaaactc gttgcagaac gtgcaaacgc taaaggtatt tcagaagtgg tgttcgaccg
    27961 cggtggatat ctatatcacg gacgtgtgaa agctttggct gatgcagctc gtgaaaacgg
    28021 attgaaattc taataggagg acactagaaa atggcattta aagacaatgc agttgaatta
    28081 gaagaacgcg tagttgctgt caaccgtgtt acaaaagttg ttaaaggtgg acgtcgtctt
    28141 cgtttcgcag ctcttgttgt tgttggtgac cacaatggtc gcgtaggatt tggtactggt
    28201 aaagctcaag aagttccaga agcaatccgt aaagcagtag atgatgctaa gaaaaacttg
    28261 atcgaagttc ctatggttgg aacaacaatc ccacacgaag ttctttcaga attcggtgga
    28321 gctaaagtat tgttgaaacc tgctgtagaa ggttctggag ttgccgctgg tggtgcagtt
    28381 cgtgccgttg tggaattggc aggtgtggca gatattacat ctaaatcact tggttctaac
    28441 actccaatca acattgttcg tgcaactgtt gaaggtttga aacaattgaa acgcgctgaa
    28501 gaaattgctg cccttcgtgg tatttcagtt tctgatttgg cataagaaag gggataaaat
    28561 ggctcaaatt aaaattactt tgactaagtc tccaatcgga cgcattccat cacaacgtaa
    28621 aactgttgta gcacttggac ttggcaaatt gaacagctct gttattaaag aagataacgc
    28681 tgctatccgt ggtatgatca cagcagtatc tcacttagta acagttgaag aagtaaacta
    28741 atgaagtttt aggggatgtg cactgtacca tcccctaaaa ctagatatag tcatctatga
    28801 tgacatcgta taggcgagtt gatgggggag acaacctttt ctcccttatc ggcgctagca
    28861 ttttacaaaa gaggagaaaa taaaaatgaa acttcatgaa ttgaaacctg cagaaggttc
    28921 tcgtaaagta cgtaaccgcg ttggtcgtgg tacttcatca ggtaacggta aaacatctgg
    28981 tcgtggtcaa aaaggtcaaa aagctcgtag cggtggcgga gttcgccttg gttttgaagg
    29041 tggacaaact ccattgttcc gtcgtcttcc aaaacgtgga ttcactaaca tcaacgctaa
    29101 agaatacgca attgtgaacc ttgaccaatt gaacgtcttt gaagatggtg ctgaagtaac
    29161 tccagttgtt cttatcgaag caggaattgt taaagctgaa aagtcaggta ttaaaattct
    29221 tggtaacggt gagttgacta agaaattgac tgtgaaagca gctaaattct ctaaatcagc
    29281 tgaagaagct atcactgcta aaggtggttc agtagaagtc atctaagaga ggtgacctat
    29341 gttttttaaa ttattaagag aagctcttaa agtcaagcag gttcgatcaa aaattttatt
    29401 tacaattttt atcgttttgg tctttcgtat cggaactagc attacagttc ctggtgtgaa
    29461 tgccaatagc ttgaatgctt taagtggatt atccttctta aacatgttga gcttggtgtc
    29521 ggggaatgcc ctaaaaaact tttcgatttt tgccctagga gttagtccct atatcaccgc
    29581 ttctattgtt gtccaactct tgcaaatgga tattttaccc aagtttgtag agtggggtaa
    29641 acaaggggaa gtaggtcgaa gaaaattgaa tcaagctact cgttatattg ctctagttct
    29701 cgcttttgtg caatctatcg ggattacagc tggttttaat accttggctg gagctcaatt
    29761 gattaaaact gctttaactc cacaagtttt tctgacgatt ggtatcatct taacagctgg
    29821 tagtatgatt gtcacttggt tgggtgagca aattacagat aagggatacg gaaacggtgt
    29881 ttccatgatt atctttgccg ggattgtttc ctcaattcca gagatgattc agggcatcta
    29941 tgtggactac tttgtgaacg tcccaagtag ccgtatcact tcatctatca ttttcgtaat
    30001 cattttgatt attactgtat tgttgattat ttactttaca acttatgttc aacaagcaga
    30061 atacaaaatt ccaatccaat atactaaggt tgcacaaggt gctccatcta gctcttacct
    30121 tccgttaaag gtaaaccctg ctggagttat ccctgttatc tttgccagtt cgattactgc
    30181 agcgcctgcg gctattcttc agtttttgag tgccacaggt catgattggg cttgggtaag
    30241 ggtagcacaa gagatgttgg caactacttc tccaactggt attgccatgt atgctttgtt
    30301 gattattctc tttacattct tctatacgtt tgtacagatt aatcctgaaa aagcagcaga
    30361 gagcctacaa aagagtggtg cctatatcca tggagttcgt cctggtaaag gtacagaaga
    30421 atatatgtct aaacttcttc gtcgtcttgc aactgttggt tccctcttcc ttggtgtgat
    30481 ttccatttta ccgattgcag ctaaagatgt atttggtctt tctgatgttg ttgcctttgg
    30541 tggaacaagt ctcttgatca ttatctctac aggtatcgaa ggaatcaagc aattggaagg
    30601 ttacctattg aaacgtaagt atgttggttt catggacaga acagaataaa agtatttact
    30661 gaatcagtaa atgctgaggg agtggaggtt taaactctga catttgtaag agttggatct
    30721 cccctcttct attttgtttt taaatagggg tgaaaagact ttttgcttct atttaaaaat
    30781 aaaataagga gatcaaatca tgaatctttt gattatgggc ttacctggtg caggtaaggg
    30841 aactcaagca gcaaaaatcg tagaacaatt ccatgttgca catatctcaa caggtgatat
    30901 gttccgcgct gcaatggcaa atcaaactga aatgggtgtt cttgctaagt catatattga
    30961 caagggtgaa ttggttcctg acgaagttac aaatggaatc gtaaaagaac gcctttcaca
    31021 agatgatatt aaagaaacag gattcttatt ggatggttac ccacgtacaa ttgaacaagc
    31081 tcatgccttg gacaaaacat tggctgaact tggcattgaa ctagaaggtg ttatcaatat
    31141 tgaagtgaac cctgacagcc tcttggaacg tttgagtggc cgtatcatcc accgcgtaac
    31201 tggagaaact ttccacaagg tctttaaccc accagttgac tataaagaag aagattacta
    31261 ccaacgtgaa gatgataagc ctgagacagt aaaacgtcgt ttggatgtta atattgctca
    31321 aggagaaccg atcattgctc actaccgtgc caaaggtttg gttcatgaca tcgaaggtaa
    31381 tcaagatatc aatgatgtct tctcagatat tgaaaaagta ttgacaaatt tgaaataaag
    31441 cgtttttcat acttgcaaaa attcgctaca aatgttatac tgagatagtc tgacttataa
    31501 ttgttgtctc tgtgtctaga ggcatcgaat cgaaatttat ggaggtgctt ttgcgtggca
    31561 aaagacgatg tgattgaagt tgaaggcaaa gtagttgata caatgccgaa tgcaatgttt
    31621 acggttgaac ttgaaaatgg acatcagatt ttagcaacag tttctggtaa aattcgtaaa
    31681 aactatattc gtattttagc gggagatcgt gttactgtcg aaatgagtcc atatgacttg
    31741 acacgtggac gtatcactta ccgctttaaa taatcgaaaa acttggaggg ataagaaatg
    31801 aaagtaagac catcggtcaa accaatttgc gaatactgta aagttattcg tcgtaatggt
    31861 cgtgttatgg taatttgccc agcaaatcca aaacacaaac aacgtcaagg ataagataga
    31921 aaggagaaaa catggctcgt attgctggag ttgatattcc aaatgacaaa cgcgtagtaa
    31981 tctcattgac ttatgtttat ggtatcggac ttgcaacatc taagaaaatt ttggctgctg
    32041 ctggaatctc agaagatgtt cgtgtacgtg atcttacatc agatcaagaa gatgctatcc
    32101 gtcgtgaagt ggatgcaatc aaagttgaag gtgaccttcg tcgtgaagta aacttgaaca
    32161 tcaaacgttt gatggaaatc ggttcatacc gtggtatccg tcaccgtcgt ggacttcctg
    32221 tccgtggaca aaacactaaa aacaacgctc gcactcgtaa aggtaaagct gttgcgattg
    32281 ctggtaagaa aaaataatat aggaggtaaa agtcttggct aaaccaacac gtaaacgtcg
    32341 tgtgaaaaag aatatcgaat ctggtattgc tcatattcac gctacattta ataacactat
    32401 tgttatgatt actgatgtgc atggtaatgc aattgcttgg tcatcagctg gtgctcttgg
    32461 tttcaaaggt tctcgtaaat ctacaccatt tgctgctcaa atggcttctg aagctgctgc
    32521 taaatctgca caagaacacg gtcttaaatc agttgaagtt actgtaaaag gtccaggttc
    32581 tggtcgtgag tcagctattc gtgcgcttgc tgccgctggt cttgaagtaa cagcaattcg
    32641 tgatgtgact ccagtgccac acaatggtgc tcgtcctcca aaacgtcgcc gtgtataatc
    32701 atcgcattac actgcttttc gtttaagagg gagtaactaa atgatcgagt ttgaaaaacc
    32761 aaatataaca aaaattgatg aaaataaaga ttatggcaag tttgtaatcg aaccacttga
    32821 acgtggctac ggtacaactc ttggtaactc tcttcgtcgt gtacttctag cttctctacc
    32881 aggagcagct gtgacatcta tcaacattga tggtgtgtta catgagtttg acacagttcc
    32941 aggtgttcgt gaagacgtga tgcaaatcat tctgaacatt aaaggaattg cagtgaaatc
    33001 gtacgttgaa gacgaaaaaa tcatcgaact ggatgttgaa ggtcctgctg aagtaacagc
    33061 tggtgacatt ttgacagata gcgatattga aattgtaaat ccagatcatt atctctttac
    33121 aattggtgaa ggttcttctc taaaagcgac tatgactgtt aacagtggtc gtggatatgt
    33181 acctgctgat gaaaataaaa aggataatgc accagttgga acacttgctg tagattctat
    33241 ttatacacca gttacaaaag tcaactatca agtggaacct gctcgtgtag gtagcaatga
    33301 tggtttcgac aaattaaccc ttgaaatctt gacaaatgga acaattattc cagaagatgc
    33361 tttagggctt tcagcacgta ttttgacaga acatcttgat ttgtttacaa atcttactga
    33421 gattgctaag tcaactgaag tgatgaaaga agctgatact gaatctgacg accgtatttt
    33481 agatcgtacg attgaggaac tggacttgtc tgtgcgttca tacaactgtt taaaacgtgc
    33541 cggtatcaat actgtgcatg atttgacaga aaaatctgaa gcagagatga tgaaagtacg
    33601 aaatcttgga cgcaagagtt tggaagaagt gaaactcaaa ctcattgatt tgggtcttgg
    33661 attaaaagat aaataaagga ggaatacatg gcttaccgta aactaggacg cactagctca
    33721 caacgtaaag caatgcttcg cgatttgaca actgaccttt tgatcaacga atcaatcgtg
    33781 acaactgaag ctcgtgctaa agaaatccgt aaaactgttg aaaaaatgat tactctaggt
    33841 aaacgtggtg atttgcatgc acgtcgtcaa gcagctgctt tcgtacgtaa tgaaatcgca
    33901 tctgaaaact atgatgaagc aactgataag tacacttcta ctacagcact tcaaaaattg
    33961 ttctcagaaa tcgcacctcg ttatgctgaa cgtaacggtg gatacactcg tatccttaaa
    34021 actgaaccac gtcgtggtga tgcagcgcca atggcgatca tcgaattagt ataaaatcat
    34081 caattttgtt gagtgttatg atgatggagt cttgtgctct tagtctagct ctggtctacc
    34141 gctaggattt cggtcctagc gggaacactc atcataagtt gggatagtag acgcttgttt
    34201 acgaaattgt ttttttctta agaacaactt cgtaagcagg cgtttttgag tattttcgtt
    34261 agaattatgc tatactattt gaaaagaatc ctgtttaatg ttaaggtttc ttattttaag
    34321 aagaattgga gtttacttat gaaagcgatt ataactgttg ttggtaaaga taaatctgga
    34381 attgttgcag gtgtttctgg taaaattgca gaattaggat tgaatattga cgatatctct
    34441 caaactgtat tggatgaata ttttacgatg atggctgttg tatctagtga tgaaaagcaa
    34501 gattttacct atcttcgtaa tgaatttgaa gcttttgggc aaactttgaa tgtaaaaatc
    34561 aatattcaga gtgcagcgat tttcgaagct atgtataata tctaggaggt catcatggat
    34621 attagacaag ttactgaaac catcgccatg attgaggagc aaaacttcga tattagaacc
    34681 attaccatgg ggatttctct tttggactgt atcgatccaa atatcaatcg tgctgcggag
    34741 aaaatctatc aaaaaattac gacaaaggcg gctaatttag tagctgttgg tgatgaaatt
    34801 gcggctgagt tgggaattcc tatcgttaat aagcgtgtat cggtgacacc tatttctctg
    34861 attggggcag cgacagatgc gacggactac gtggttctgg caaaagcgct tgataaggct
    34921 gcgaaagaga ttggtgtgga ctttattggt ggtttttctg ccttagtaca aaaaggttat
    34981 caaaagggag atgagattct catcaattcc attcctcgcg ctttggctgc gacggataag
    35041 gtctgctcgt cagtcaatat cggctcaacc aagtctggta ttaatatgac ggctgtggca
    35101 gatatgggac gaattatcaa ggaaacagca aatctttcag atatgggagt ggccaagttg
    35161 gttgtattcg ctaatgctgt tgaggacaat ccatttatgg cgggtgcctt tcatggtgtt
    35221 ggggaagcag atgttatcat caatgtcgga gtttctggtc ctggtgttgt gaaacgtgct
    35281 ttggaaaaag ttcgtggaca gagctttgat gtagtagccg aaacagttaa gaaaactgcc
    35341 tttaaaatca ctcgtatcgg tcaattggtt ggtcaaatgg ccagtgagag actgggtgtg
    35401 gagtttggta ttgtggactt gagtttggca ccaacccctg cggttggaga ctctgtggca
    35461 cgtgtccttg aggaaatggg gctagaaaca gttggcacgc atggaacgac ggctgccttg
    35521 gccctcttga acgaccaagt taaaaagggt ggagtgatgg cctgcaacca agtcggtggt
    35581 ttatctggcg cctttatccc tgtttctgag gatgaaggaa tgattgctgc agtgcaaaat
    35641 ggctctctta atttagaaaa actagaagct atgacggcta tctgttctgt tggattggat
    35701 atgattgcca tcccagaaga tacgcctgct gaaaccattg cggctatgat tgcggatgaa
    35761 gcagcaatcg gtgttatcaa catgaaaaca acagctgttc gtatcattcc caaaggaaaa
    35821 gaaggcgata tgattgagtt tggtggtcta ttaggaactg cacccgttat gagggttaat
    35881 ggggcttcgt ctgtcgactt catctctcgc ggtggacaaa tcccagcacc aattcatagt
    35941 tttaaaaatt aagaaaatag gagaaatttt aagttctatt taagattaga tgtgtatact
    36001 ataatcatta aataaagacc tcctaatatt atttgaaaca gataacactg aattagtttg
    36061 aatttgattt tcatctaata tctttattta atgaactcct aaactttttc ataataatct
    36121 ccttcaaaag tcgcctgtat gggtggcttt tattttatca ttcatgatat aatagaagca
    36181 aacggaggac ggaaaatggt aaaagtacga ttgtatttgg tacgtcatgg caagaccatg
    36241 tttaacacga ttggtcgcgc gcaaggttgg agcgatactc ccttaactgc tgaaggtgaa
    36301 cgagggattc aagagttagg aattggtttg cgagaatctg atctacagtt tgagcgcgct
    36361 tattcgagtg attctggtcg taccattcag accatgggaa ttatccttga agaacttggc
    36421 ttgcagggga aaatccctta tcgcatggac aagcgtatca gagaatggtg tttcggtagt
    36481 tttgatggag cctatgatgg cgatcttttc atgggcatta ttcctcgtat ctttaatgtg
    36541 gaccacgttc accaattgtc ttatgctgaa ctggctgagg gcttggtaga ggtcgataca
    36601 gctggttggg ctgaaggctg ggaaaaactc agtggccgaa tcaaggaagg ctttgaaatg
    36661 attgcaaaag aaatggaaga tcaaggtgga ggtaacgccc ttgttgtcag ccatggaatg
    36721 actattggaa ccattgttta tctgattaat ggcatgcatc cgcatggtct agataatggt
    36781 agcgtgacga ttcttgaata tgaggacggt cagtttaggg ttgaagttgt cggtgaccgt
    36841 agttaccgag agctaggacg tgagaagatg gaagaaggct ctatttaatc agtctagact
    36901 tgcttgccat gagctaggga tttgataaga atatcaagat aagaaaaaac agccgagggt
    36961 actcctttcg gctgtttttg atgtggaaaa ctaaagtgta atgctattgc ttttagagat
    37021 tttcataaac aagagcaagg aacctactgt tagaacagtc aggatagttg acaaggctgc
    37081 ggctacaccg taatttcctc tgagaacctc tgtataaata gctacagtca ttgttcttgt
    37141 tttgacattg tagaggagga tagaagtaga gagttttgaa atcagtgtga cccaagataa
    37201 gatggctcca gaaataatac ccgatagcat cattggagtt gtaatcttag caaagatatt
    37261 gagacgacta cttcctaagc tttcagcagc ttcttcaata cttggtgcta tttgttgtaa
    37321 gctagcaaca gatgagcgaa tagtatacgg taatcttctg gcagatagag acataatcaa
    37381 gatgaaagca gtccctgtaa tcataagaaa tccacttcca aatagaccag tattgaagga
    37441 agaaatgaag gcaatcccta gaacggttcc tggtacaata taaggtacca tactgaggct
    37501 gtcaattaag tttgtaaaca aattccgttt tctaacggct aggtaggaga taaatgtcgc
    37561 aaatagaaca actagaacta aggcaatcaa agggatacga atggtattga aaatagcaga
    37621 tcccatacga ttgaaagcta gcttgtaact gtttggagaa taacctttaa caaataccat
    37681 acctgatgtt tttaggaaag aggtataaat taagtagatt tgaggtaaaa cagagataaa
    37741 gataattccg tagactgttg cataaatggc agccattttt ccttttgtag tttttttagg
    37801 ctcaattgga tggagcggat tcatgctgaa actgtagcgg tttgcaatgt gtttttggat
    37861 aaggaaaatt gccaaggcaa tgataatcgc cataattgca aaagcagaat ttcctccaac
    37921 ctcgctaata aattgggtat aaatcaggac agggaaagtc cgatatcctt cgccaatcaa
    37981 cataggcgtt ccaaagtctg agaatgctct cataaataca agcaaggagt tgctagtaag
    38041 gttggaacta ggagaggtaa aacaaccgtt acgataggtt taaatccgaa ggaccccatg
    38101 ctttcagctg cttcaagtag agaattgtca atactgttca ttgttccagc aacatataga
    38161 aataccagtg ggaatagttg cagtgtaaag acaagtataa ttcctttgaa tccataaata
    38221 tcgatagctg gaagatgaag ggcatttgtc aaaaatttag tgatgacctc atttcgtccc
    38281 agcaagagaa tccaggagta ggctcctacg aaaggagctg acatggaagc aatgataatc
    38341 aatatttgta ggaatttctt ccccttgaag tcatacatag agaagagata agctaatagg
    38401 gttcctacaa ctaaggaagt gacagtagcg gtaatggaaa ccctgaaact gttgactagt
    38461 gtctcagagt agtaggtttt actaaagaaa gtgacaaaat tagctagtga gaattatcct
    38521 tcatgtataa gtgcttactt gagcacggta acgataggat aaacgagaaa gacaagatag
    38581 gtaagaaaga ggaggctgtc caaatattta gttttttacg ttccatggtt gactcctttt
    38641 atcaggtttt gggaaccatc tgcagaaaag atgtttaatt tttgcgtatt gattcgtaga
    38701 cgaatacgat tgtctttttg tagatcttct tcaaaagttg attcttcact aacttgaatt
    38761 tttgaggcaa aacctgtctc aatgaaatac tccgtattta gtccaagata gacgctatcg
    38821 ctaatagttc cttcgatatc tccagattca tctttgataa actcttcggg acgaatgctt
    38881 acatgaatag cttgctcctc aacctgatca agagctagca ttcgaagggc atagccatct
    38941 gaaaagacga tataagcgcc gtcgctccgt ttttcaagat tggcagggat aatatttgtg
    39001 cgtccgataa aggttgccac aaactcatta gttggtttat gatagagttc ttttggtcgg
    39061 ccgatttgtt ggatcacccc atctttcata acagcaattt ggtctgaaat agccatggct
    39121 tcttcttggt cgtgggttac ataaacagtt gtaattccca cttcgtgttg gatttctcgg
    39181 atggcttgac gcatatccaa gcgaagtttg gcctccagat tactaagtgg ctcgtccatg
    39241 aggagaacac ttagattaac cgctaaggcg catgccaagg tgacacgttg ttgttgtcca
    39301 ccactgagtt tatcgggctt tcgatccgca tattgagcaa tttgcatgag ttcaagatac
    39361 ttgttggtct gttgaatcaa ttcttctttt ggaaccttct tttgcataag accaaaagca
    39421 acgttgtctc ggacagtcaa atgtgggaaa atagcgtagt tttggaaaac catcccgata
    39481 ttgcgtttgc tgggttccat attattgatt tttgtatcat cgaagtaaaa ttctccatct
    39541 ttgatactgt tgaaacctgc aatcatacga agaagggtcg ttttcccaca tcctgaagct
    39601 ccaagaaggg taaagagact tccttttgga attgtaatgt tcaaattctc aataacaggg
    39661 acatcgtggt agattttttt ggcgttaata atttcgatct cactcatagt gaacctcttt
    39721 tactgtttag attggatatc tgtaaagact tcgttgtatt tcttaacgat atctgattta
    39781 ttcttgatga cataatcata atcttcagtg agtgttttga ttttgtcaat tggtttcatg
    39841 ttttcgcttg ttttagcatt tttacgaaca ggacggttag tagtggttgt accaagtgta
    39901 tcttgtactt cttgagagat aataaaatcg ataaatttct tggcattttc catattttta
    39961 gcttttttaa cgatagcagc actagcaggt aggaagacgg ttccttcttt tggatagact
    40021 accttaatgt tagctccgtc atttaagagt ttaactgctg gatcttcata agagagacca
    40081 acagccattt ctccatcagc gactgcttta tagacactag atgaacttga accgatttta
    40141 ccatcaataa gtgtgaaaag atcttttaca taagaccaag ccttatcatc tttgtaacca
    40201 ccttgagctt gtagcatatt tgttaattga gcaaaggcgc tagaagagtt tgctgggtca
    40261 gcagttgcga tttttccttt tagttcaggt ttgagaagat cgttatatcc ttcgatgttc
    40321 atgcctttag ttaaatcagg gttgacgatt aaaacactac catctagtgt ataaggagta
    40381 gagtagccag ttgtgttttg atattctttg ataacattat cattttcttt tgaagtatag
    40441 ttttcaaaga gttctccgtg ggtagcatat tgtgtataag aaccaccaaa gataacatca
    40501 gctacaggaa cttctttttc tgactctagt tttttgaaaa gttctccagt accagcttga
    40561 atcagttcta ttttgatacc atatttttct tcaaaggcag gaatagttgc tccaattaag
    40621 ccttctgagt ttggtgaata aacgactagc gaaccaccgt ctcctttatc agatgaactg
    40681 tcatcggcag attcattaga agaacaagca gcataataca tccatttctt tttcatgatg
    40741 gatacctccg ttgtgttatt taagtttatt ttaaaacaat gtaagcgttt ttaaaaccta
    40801 caattctatt ctatagtgta ttgaatctat aacagtacac tttgactgct aaaatatttc
    40861 tataaattaa tttgactttc ctgatagaga tgttcacatc ttatttcaat tcactatatt
    40921 agagtaaaat tctctacaaa aagaagaata gcctatttga ctattcttcc gagtgatttc
    40981 aatttctttg gggaaatatg gagatacttt ttaaatactt tggcaaaata tttatagtct
    41041 gaaaatccaa cttggtcaga gatagcgctt aaaggggagt tgggagattc agccatctta
    41101 tctatagctt gtttcaggcg atattgtaag atatattcat ttagagtcat ggggaagtct
    41161 tctttgataa ccttatatag atgcttgtta tctgcaaata gctagagcat ttcaaggaaa
    41221 taaactagaa ataacaatta atatggtggt tcagaaatac cttttgataa tcaataagat
    41281 cttcaggatg aagtgcgttg attccatcca tttcatattt ccgatttaat aaacgatatt
    41341 tgttttcacc aaattgatga aaagggagta gttgaacttg gtcgatattt aatgagttaa
    41401 atagagtagc gaatttttct gcatcctcta aactattgtt aaaattagga ataactggga
    41461 ttcttaaaac gatagtttta ttttgagaaa aagcataatg aatgttttta ataatcattt
    41521 gattaaaaac cccagtcact tttttatgtt ttatagaatt ataatgtttt aggtctgtgt
    41581 agataaaatc cacatattga attaaatcga taaatttttc atgatcaaca aaggcagtag
    41641 tttcaatggc agtgtgtatg tgatgttctt tagctgattt taagatggct ttagcaaatt
    41701 caaactgagc aaatatttca cctcctgata aagttaaacc tccaccggat tcttcgtaaa
    41761 attctttgtc ttttaatacc tctgtaataa tttcttctac agtcttttct tcaccgacta
    41821 aggtgaattt ctctcgttga gcatctttca ttttttcagg tttcattctt tgagattcag
    41881 gattagaaca ccatggacag cgcagaggac atccttttaa aaaaacagtt gtacgaatac
    41941 ccgggccgtc atgaattgaa aagtgttgaa tattaaaaat aattccttta gatatttcca
    42001 taagagttcc tcctcttcat aagtctatcc tatcacaaaa acgaaagaaa aacaattacg
    42061 aacgaaaggt taatttgttg ttttttgctt tttattttgg tagaataaaa cggaggtgta
    42121 aaatgaaaag attggaacaa attattaaat tagtatcaga atatgaaaag atcgacgtta
    42181 atacattatc ggaaaaatta aatgtatcga aagtaacgat tagaaaagat ttagataaat
    42241 tagagtcaaa aggtttatta cacagagagc atggatatgc tgtattaaat agtggagatg
    42301 acttaaatgt acgtttgtca attaattatg aaattaagag aaaaattgtt caggaagcag
    42361 taaaattggt gtcagataat gaaacaataa tgatagaatc tggatcgacc tgtgctttac
    42421 ttgctgagga aatttgcaag caaaaaagaa atgttacgat tgtaacaaat tcatttttta
    42481 tagcaaattt tgtgagagct tatgattcat gtcgtattat tgttcttggt ggagagtttc
    42541 agaaggattc acaggttact gtgggacctt tattaaaaga aatgatacag acttttcatg
    42601 tgcgtcaagc ttttgttggg acagatggct acgataaaga gatgggcttt acaggaaaag
    42661 atttaatgcg cagtgaggta gttcaatata tttcagcagc gtcggataaa gtcattgtac
    42721 taactgactc aagtaaattt gataaaaggg gtacagtaag aagatttgct ttaagtcaag
    42781 tctatgaagt aataacagac gaaaaacttt ctaaacaaaa tatagctaca ttagaaaatg
    42841 ctgggataat ggttaaggta gtttcgtaag aggttaagtg tatgaatcaa gataggaata
    42901 aactgctttc taaaattgct tatctgtatt atattgaaaa cttaaatcag tcacaaatag
    42961 cagcaaaatt aggaatttat agaacctcta ttagtagaat gttaacagaa gcaaggaatg
    43021 caggaattgt taaaattgaa atagagaatt ttgatacaaa tatgtttaag ttggaaaatt
    43081 atgtaaaaga aaaatatggt ctggaaagtt tagaaattat tccaaatgaa tttgatgata
    43141 atccaacaat tttatctgaa agaatttctc aagttgcagc aggtgtcctt aggaatttaa
    43201 ttgatgataa tatgaaaatt ggcttttctt gggggaaaag tttaagtaat ttagtagatt
    43261 taattcacag taaaagtgtc cgaaatgttc acttctatcc tctagcaggt ggtcctagtc
    43321 acatacacgc taaataccat gtgaatacac tgatttatga aatgtctaga aaatttcatg
    43381 gagagtgtac atttatgaat gcaacgattg tgcaagaaaa taaattgtta gcagatggtg
    43441 ttttgcaatc aagatatttt gaaaatttga aaaatagttg gaaagattta gatatagctg
    43501 tagtcggaat tggtgatttt agcaataaag gaaaacatca atggttagac atgcttacag
    43561 aggatgactt taaagaattg accaaagtta aaactgtagg agaaatttgt tgtcgatttt
    43621 ttgattcaaa aggtaaagaa gtctatgaaa acttacagga aagaacgata gcaatctctt
    43681 tggaagattt aaaaaatatt cctcaaagtt tagctgttgc ttacggtgat acgaaagtat
    43741 cttcgattct ttctgtcttg cgtgctaatt tagtaaatca tttgattaca gataaaaata
    43801 caattttaaa agttttggaa gaagatgggg atttgacttt tagagagatt ctaggtgagt
    43861 gaaaatgata gactgatcag tttatcgttt tttcttttta gtcgattgca catttgtgct
    43921 catataaaca aaaatagttt atctgttgtt tttggattga caactttatt atgtagttgt
    43981 attctatagt tacaaaagaa aattttaaaa tttcaaatga aaaaagcttt ttacatagtg
    44041 aaatgaggag gaatttatgg aaattattgt tccagatcaa attatcatgg gtttaatttt
    44101 atatgctggt gatgcgaaac aacatattta taaagcgtta gattacataa aaaatggtac
    44161 atgtgaacgg tgtgaagaag aaatccagtt agctgatgcc gccttattag aagctcataa
    44221 tctacaaaca aaatttttgg cacaggaagc gtctggtaca aagacagaaa ttacagctct
    44281 ctttgttcat tcacaagatc atctcatgac tagtatgacg gagattaatt taatcaaaga
    44341 aattattagt ttgagaaaag aacttcataa aaaataatac tagagtatta tcattgttat
    44401 taacatagag gaggaaaaca taatggtgaa gattggtttg ttttgtgcag caggtttttc
    44461 tactggtatg cttgtaaata atatgaaaat tgcagcgcaa gctagtggag ttgaggcaga
    44521 aatagaggcg ttttctcagt ctaaattagc ggattatgcg ccaaatatag atgttgcact
    44581 attgggtcca caagttgctt atacattaga taaatcaaaa gaaatttgtg ataagtgtga
    44641 tgttccgata gctgttattc cgatgatgga ctatggtatg ttagatggga aaaaagtatt
    44701 agatttggcc ctatctttga ttagtgggta agaaaaggag atttattatg tcaaagatgg
    44761 atgttcagaa aatcattgca ccgatgatga agtttgtgaa tatgcgtggc attatagctc
    44821 taaaagatgg gatgttagca attttgccat tgacagtagt tggtagtttg ttcttgatta
    44881 tgggacaatt gccgttcgaa ggattaaata agagcattgc tagtgttttt ggagctaatt
    44941 ggacagagcc gtttatgcaa gtatattcag gaacttttgc tattatgggt ctaatttctt
    45001 gtttttcaat tgcctattct tatgctaaga atagcggagt agaggcttta ccagctggag
    45061 ttctatctgt atctgcattc tttattttgc taagatcatc ttatatccct aaacaaggtg
    45121 aggcgattgg ggacgctatt agtaaagttt ggtttggagg ccaaggaatt atcggtgcta
    45181 tcattatagg tttggtagta ggaagtattt ataccttctt tataaagaga aaaattgtta
    45241 ttaagatgcc agaacaagtt ccacaagcta ttgccaaaca gtttgaagca atgattccag
    45301 catttgtaat tttcttactt tctatgattg tatatatttt agcgaagtca ttgactaatg
    45361 gcggaacatt catagaaatg atttattctg ctattcaagt tccattgcaa ggtttaactg
    45421 gatcttggta tggtgcaatt ggaattgcat tctttatatc atttttgtgg tggtttggtg
    45481 ttcatggaca atcggtagta aatggagtag tgacagctct gcttttatct aatcttgatg
    45541 ctaataaagc tatgttagcc tctggtaatc tatcattaga aaatggtgca catattgtta
    45601 ctcaacaatt tttagattca tttttaattc tatcaggttc agggattacg tttggtcttg
    45661 tagttgccat gctttttgca gcaaaatcaa aacaatacca agccttagga aaagttgccg
    45721 cttttccagc aatatttaac gtaaatgagc cagttgtatt tggatttccg attgtcatga
    45781 atccagttat gtttgtacct ttcattcttg ttcctgtact tgcagctgtg atagtatatg
    45841 gagctattgc aacaggtttc atgcagccat tctcaggggt aacattgcct tggagtacac
    45901 cagctatttt atcaggattt ttggtgggtg gatggcaagg agttattact cagctggtga
    45961 tattagcgat gtctacattg gtttattttc cattctttaa agtacaggat cgtttagctt
    46021 accaaaatga aatcaaacaa tcttagaggt atttgtgtgt tactgttaaa ctcacacatt
    46081 tgtgctaaaa attagagagt taaaattttt ctagttaaaa gcttgaaaat ttctataaaa
    46141 atcggtatta tattttcgaa agaaataaaa atattttcga aagaaaggtg gttacgatgg
    46201 taaatacaga agtagcaaga acaacaatca agacagaata ttttggcagc cttactgaaa
    46261 ggatgaacaa atatcgagaa gatgttttaa ataaaaaacc ttatattgat gctgagagag
    46321 cagttctagc aacacgcgcc tatgaacgat acaaggaaca acctaatgtc ctaaaacgtg
    46381 catatatgct gaaagaaatt ttggaaaata tgactatcta tattgaagaa gaatctatga
    46441 ttgggggaaa tcaagcttct tccaataaag atgctcctat ttttccggaa tatacgctag
    46501 aatttgttct caatgagttg gatctttttg aaaagcgtga tggagatgtt ttctatatta
    46561 cagaagaaac aaaagaacaa cttagaagta ttgctccgtt ttgggaaaat aataatttac
    46621 gtgctagagc tggtgcctta ttacctgaag aagtgtctgt ttatatggaa acaggattct
    46681 tcggtatgga aggtaagatg aattctggag atgctcacct agcagttaac tatcagaaac
    46741 ttttgcaatt tggcttaaga ggttttgaag agcgggctcg taaagcaaaa gcagctctag
    46801 atttaacaga tccagcaagt attgataaat atcattttta cgactctata tttatcgtaa
    46861 tcgatgctat taaagtatat gcaaagcgct ttgttgctct tgctaaaagt ttagccgaaa
    46921 atgcaaatcc taaacgtaag aaagaattac ttgagattgc agatatttgc tctagagtcc
    46981 catatgaacc ggcaactact tttgcagaag ctattcaatc agtttggttt attcaatgta
    47041 ttttacaaat tgaatctaat ggccactctc tttcatatgg ccgttttgat caatatatgt
    47101 atccatatat gaaggctgat ttagaaagtg gtaaagaaac agaagatagc attgttgaac
    47161 gtctgacaaa tctttggatt aagacaatta caattaataa ggttcgcagt caatcacata
    47221 cattttcttc agcaggaagt cctttatatc aaaatgttac aattggtgga cagactcgag
    47281 ataagaagga tgctgttaac ccattatctt atttggtatt aaaatcagtt gcacaaaccc
    47341 atctaccgca acctaatcta actgtacgtt accatgcagg tttagatgct cgtttcatga
    47401 atgagtgtat tgaagtgatg aaacttggtt ttggtatgcc tgcatttaat aatgatgaga
    47461 ttattattcc ttcttttatt gcaaaaggag tattggaaga tgatgcttat gattacagtg
    47521 ccattggatg tgttgaaacg gcagttccag ggaaatgggg ctatcgttgc acaggtatga
    47581 gttatatgaa cttccctaag gttctactta tcacgatgaa tgatggaatt gatccggctt
    47641 cgggtaaacg gtttgcacca agctttggtc attttaagga tatgaagaac ttttctgaat
    47701 tagaaaatgc ttgggataaa acactaagat atttgacacg aatgagtgtt attgttgaaa
    47761 attctattga tttatcattg gaacgagaag ttcctgatat tctatgttca gcattgactg
    47821 atgattgtat tggtcgtgga aaacacctta aagaaggtgg agcagtatat gattatatat
    47881 caggattgca agttggaatt gcaaatttgt cggattcatt agctgcaatt aaaaaattgg
    47941 tgtttgagga agaacgtata agcccaagtc agctttggca tgcactggaa acagattatg
    48001 ccggagaaga aggtaaggtc attcaagaaa tgttgattca tgatgcacct aagtatggta
    48061 atgatgatga ttatgctgac aaattggtta ctgctgctta tgacatttat gttgatgaaa
    48121 ttgctaaata tcctaataca cgttatggaa gaggtccaat tggaggaatt cgttattcag
    48181 gaacatcttc tatctcagcc aacgtagggc agggacgtgg aacattagca actccagatg
    48241 gacgcaacgc gggtacaccg ttagcagagg gttgttcacc atcacataat atggatcaac
    48301 acggccctac atctgtttta aaatctgttt caaaattacc aacagatgaa atcgtaggtg
    48361 gggttctctt aaatcagaaa gtaaatccta aaacgttagc caaagaagaa gataaattaa
    48421 aactaattgc tttgttacga acattcttta atcgtttaca tgggtaccat attcaataca
    48481 atgttgtttc cagagagacg ctgattgacg ctcagaaaca tcctgaaaaa cacagagact
    48541 taattgttcg tgttgcagga tactctgcat tcttcaatgt tctttctaag gcaacccaag
    48601 atgacattat aggacgtact gagcatactt tgtaaaataa agaggttctt tttatggaat
    48661 ttatgcttga cacattaaat ttagatgaga ttaaaaagtg gtctgaaatt ttgccgctag
    48721 ctggggtaac ttcaaatccc actattgcaa aaagagaggg ttctattaat ttttttgaac
    48781 gaatcaaaga tgtaagagaa ttgattggct ctacaccctc tattcatgtt caggtgattt
    48841 ctcaagattt tgaaggcatc ttaaaggatg ctcatgaaat tcgaagacaa gcaggagatg
    48901 atatatttat caaagtacct gttactccag ctggattacg tgcaataaag gtgctaaaaa
    48961 aagagggcta ccatatcact gcaacagcta tttatacagt tattcaggga ttattagcta
    49021 tcgaagcagg agctgattac ctagctccat attataatag aatggaaaat ctgaacattg
    49081 attcaaattc tgtcattcgt caattagctc ttgctattga tagacagaac tctcctagta
    49141 agattttagc tgcatccttt aaaaatgtag cacaagtaaa taatgcttta gctgcaggtg
    49201 cgcatgctgt tacagcagga gcagatgttt ttgaatcagc tttcgccatg ccatctatcc
    49261 aaaaggcggt tgatgatttt tctgacgatt ggtttgttac tcaaaatagt cgttccattt
    49321 agatagagag gaaatatata tgagaatttt tgctagtcct tctagatata ttcaggggga
    49381 aaatgccttg tttgaaaatg ccaaatcaat tttggatttg ggaaattacc ctattctatt
    49441 atgcgatcag ttggtttatg atattgttgg aaaacgattt gaagattacc tacataggta
    49501 tggtttccat attgttctgg cgctatttaa tggtgaagct tctgacaatg aaatcaatcg
    49561 agttgttgcc ttggctgaga aagaaaattg tgatagtatt atcggtcttg gtgggggaaa
    49621 gacgattgat agcgcaaaag ctattgcaga tttgattgaa aagcctgtta ttattgctcc
    49681 aacaattgca tcgaccgacg cacctgtatc tgctttatct gttatttata cagatgaagg
    49741 tgcatttgat cattatctat tttattctaa aaatccagat ttagttttgg ttgatacaaa
    49801 agttatttca caagccccta agcgtttatt agcgtctggt attgcagatg gtttagcaac
    49861 ttgggttgag gcgcgtgcgg ttatgcaggc aaatggaaaa actatgttgg gacaacagca
    49921 aacattggct ggagttgcaa ttgcgaagaa atgtgaagaa acgctgtttg cagatggttt
    49981 acaggctatg gcagcttgtg aagctaaagt ggtgacacca gcattagaaa atattgttga
    50041 agctaatact ttattgagtg gtctaggttt tgaaagtgga ggattagctg cggcgcatgc
    50101 aattcataat ggttttactg cattgacagg tgacattcat catttaacac atggtgaaaa
    50161 agtagcttat ggaactttag tacaactatt attggaaaat agacctaaag aagaacttga
    50221 taagtatatt gagttttaca aaaaaattgg tatgccaaca actctaaaag aaatgcattt
    50281 ggatcaagtt ggatatgatg atttaataaa agttggtaaa caagcaacta tggagggtga
    50341 gacaattcat cagatgccgt ttaagatttc gccttcagat gttgctcaag ctattatcgc
    50401 tgtagatgcc tatgtaaatt caaaataaac aataaggact actgttttcc aaatggtagt
    50461 cttttattga tccccgtatt gaattctata gaagattgaa ataggatgag aacaaatcga
    50521 ttgggaaagt aaaattaatt tctataaatg ttttagcaat tgtttcgtac tatttcagat
    50581 tcagtctact atacgttctt cataaatcaa aaagcgacat aggttgtcgg ctatttattg
    50641 tgaatacatt aattagcatt ccagttttat cttcggtcta aaataagtat tttgtgctat
    50701 acgagataag cgtcttgact tactccttga tttactgcat aacaatggga taaaaagtgg
    50761 gagatagagc aattcatagt catcaaaatt aatgagatac agtatacagt tttttcttta
    50821 aacacatttc aaattccctc aaaaatggta taatagtaac atcacaaaat tggagagaga
    50881 ccatgagttt ttataatcat aaagaaattg agcctaagtg gcagggctac tgggcagaac
    50941 atcatacatt taagacagga acagatgcat caaaacctaa gttttatgcg cttgatatgt
    51001 tcccttatcc gtctggagct ggtctgcacg taggacaccc agaaggttat actgcaaccg
    51061 atatcctcag tcgttacaaa cgtgcgcaag gctacaatgt ccttcaccca atgggctggg
    51121 atgcttttgg tttgcctgca gagcaatacg ctatggatac tggtaatgac ccagcagaat
    51181 ttacagcgga aaacattgcc aacttcaaac gtcaaattaa tgcgcttgga ttttcttatg
    51241 actgggatcg tgaagtcaac acaacagatc caaactacta caagtggact caatggattt
    51301 tcaccaagct ttacgaaaaa ggcttggcct atgaagctga agtgccagta aactgggttg
    51361 aggaattggg aactgccatt gccaatgaag aagtgcttcc tgacggaact tctgagcgtg
    51421 gaggctatcc agttgtccgc aaaccaatgc gccaatggat gctcaaaatc acggcttacg
    51481 cagagcgctt gctcaatgac ttagatgaac tagattggtc agagtctatc aaggatatgc
    51541 aacgcaactg gattggtaaa tcaactggtg ccaatgtaac tttcaaagta aaaggaacag
    51601 acaaggaatt tacagtcttt actactcgtc cggacacact tttcggtgcg actttcactg
    51661 tcttggctcc tgaacatgaa ttagtagacg ctatcacaag ttcagagcaa gcagaagctg
    51721 tagcagacta taaacaccaa gccagcctta agtctgactt ggctcgtaca gaccttgcta
    51781 aagaaaaaac aggggtttgg actggtgctt atgccatcaa ccctgtcaat ggtaaggaaa
    51841 tgccaatctg gattgcagac tatgtccttg ctagttatgg aacaggtgcg gttatggctg
    51901 tgcctgccca cgaccaacgt gactgggaat ttgccaaaca atttgacctt ccaatcgtcg
    51961 aagtacttga aggtggaaat gtcgaagaag ctgcctacac agaggatggc ctgcatgtca
    52021 attcagactt cctagatgga ctcaacaaag aagacgctat tgccaagatt gtggcttggt
    52081 tggaagaaaa aggctgtggt caggagaagg ttacctaccg tctccgcgac tggctcttta
    52141 gccgtcaacg ttactggggt gagccaattc caatcattca ttgggaagat ggaacttcaa
    52201 cagctgttcc tgaaactgaa ttgccgcttg tcttgcctgt aaccaaggat atccgtcctt
    52261 caggtactgg tgaaagtcca ctagctaact tgacagattg gcttgaagtg actcgtgaag
    52321 atggtgtcaa aggtcgtcgt gaaactaaca ccatgccaca atgggctggt tcaagctggt
    52381 actacctccg ctatattgac ccgcacaata ctgagaaatt ggctgatgag gacctcctca
    52441 aacaatggtt gccagtagat atctacgtgg gtggtgcgga acatgctgtt cttcacttgc
    52501 tttacgctcg tttctggcac aaattcctct atgacctcgg tgttgttccg actaaggaac
    52561 cattccaaaa actctttaac caagggatga ttttgggaac aagctaccgt gaccaccgtg
    52621 gtgctcttgt ggcaaccgac aaggttgaaa aacgtgatgg ttccttcttt catatagaaa
    52681 caggggaaga gttggagcaa gcgccagcca agatgtctaa atcgctcaag aacgttgtta
    52741 acccagatga tgtggtggaa caatacggtg ccgataccct tcgtgtttat gaaatgttta
    52801 tgggaccact cgatgcttcg attgcttggt cagaagaagg tttggaagga agccgtaagt
    52861 tccttgaccg agtttaccgt ttgattacaa gtaaagaaat ccttgcggaa aacaatggtg
    52921 ctcttgacaa ggcttacaac gaaacagtca aagctgttac tgagcaaatt gagtctctca
    52981 aattcaacac agctattgcc caacttatgg tctttgtcaa tgctgctaac aaggaagata
    53041 agctttatgt tgactatgcc aaaggcttta ttcaattgat tgcaccattt gcacctcact
    53101 tggcagaaga actctggcaa acagtcgcag aaacagatga gtcaatctct tatgtagctt
    53161 ggccaacttg ggacgaaagt aaactggttg aagacgaaat cgaaatcgtt gtccaaatca
    53221 aaggtaaagt ccgtgccaaa ctcatggtcg ctaaagacct atcacgtgaa gaattgcaag
    53281 aaattgctct agcggatgaa aaagtcaaag cagaaattga cggtaaggaa atcgtgaaag
    53341 tgattagtgt accaaataaa ttggttaata ttgttgtaaa ataaccaatt tatttgctga
    53401 tttccaacct caaacagttt cccaaactgt ttgagccaac taaactcgtt aatatcgttg
    53461 tgaaataagg taggaatcct tcagagtaga atctggagga ttttttgaat cttcttatga
    53521 aagtatggta tactatgggc aactataaag tttgaaaagt gaaaaaagga gaaagctatg
    53581 ccagtaaatg aatatggtca aatgattggg gagtcaatgg aaggttatac accaggtgaa
    53641 ctgccttcta ttgatttctt agaagggcgt tatgctagga tagaggctct ttcagtggaa
    53701 aagcatgcgg aggatttgct agctgtttat gggcctgata cgcctcagga gatgtggacc
    53761 tacctttttc aggagccagt agcagatatg ggggagctgg ttagcctttt acatcagatg
    53821 ttggctcgta aggaccgttt ttactatgca atcatagaca aggcgactgg taaggctttg
    53881 ggatcttttt ctctcatgcg cattgaccag aataaccgag taatagaagt cggagcagtc
    53941 actttttctc cagagctaag gggtacacgg ataggaactg aagctcagta tctcctagct
    54001 cgctatgttt ttgaggagct aaactatcgt cgctacgagt ggaaatgcga tgccttaaat
    54061 ctgccatcca gacgagctgc ggagcgtttg agctttattt atgaaggaac cttccgtcag
    54121 gcagtggttt ataagggacg tacgagagat acggattggt tgtctatgat tgataaggac
    54181 tggcctcaag tcaaagatcg tttggaaaca tggttgcgtc ctgaaaattt tgataaaaat
    54241 ggacagcagt acaagagctt gagagaactt taagaggtgt tgagatgatt actattaaaa
    54301 agcaagaaat tgtcaagcta gaggatgttt tacatctcta tcaggctgtc ggttggacaa
    54361 attataccca tcaaccagag atgctggagc aggccttatc tcattcatta gtaatttatc
    54421 tggcacttga tggtgatgct gtggtgggct tgattcgttt ggttggagat ggattctcat
    54481 cagtattggt tcaggattta atcgttttac caatctatca gcgtcaagga attggtagtg
    54541 ccttgatgaa agaggcttta gaggattaca aagatgccta tcaagtccag ctggtgacag
    54601 aagagataga aagaaccttg ggcttctatc gttctatggg ctttgaaatc ttatccacct
    54661 ataattgtat agggatgact tggatgaatc gtaaaaaata acaaaacttg ttttttctta
    54721 agcaaaattt aaggatggtc tagtattata tagtcattaa ataaagacct cctaacttta
    54781 tttaataaaa tcctaaactt ttttcatcac aatctcctaa tgaagccacc caatcaggtg
    54841 gcttttttgc gtggtgaggt gctggtgata gaaatttttt gcaaaatagt aaaattttag
    54901 aaaagttaag ctagttttaa gtttcgtctt gtatcatgta gttattaaat aaagacctcc
    54961 taactttatt taataaaatc ctaaactttt ctttttcata ataatctccc ttaacttcac
    55021 ccaatcaggt ggagtttttt agctctattt caggcttttg gggactattc taaaaataat
    55081 ttttcgatat ttttcggtat ttttcggatt ttggtcgggg aattggcggg gactttttta
    55141 gcgaatataa ctaagaaata ggtctgtggt cgcttcagct agttcaaact caatttgatt
    55201 gtaagtgact gttaattgaa gaactggaac tactgtaata atgattttgc ttgtctagta
    55261 gtggcgtatt aaatgagaag tgacatgtaa gattgtctct tcgtttacga aatcaaaatt
    55321 tctatgaaat tgatagaatc cctaacgttc tcttaatctt aaccgtcttg tcgtacagcg
    55381 gttgaagaaa atgtaaacct ttgacaaaat tcgtgaacta tgggataata aaaaggaatt
    55441 gagtactagt tctatatcat aggctagaaa agaccccaag ctcacgacca aataagcttg
    55501 aggtcttttt tgacactatt tgtccttgtt tagccattta tcgactatac gaagaacgac
    55561 accgaccaca attggtccga tgatagtttt gagtactaat tccatcatgg gctatctcac
    55621 ctcctttcgt aggcggtgta gcagtgtcgt agaatattat accacataag tgctaaactt
    55681 aggagtcacc caatcaggtg gcttttttgt ttgtggcttg gttttttgat ataatagagc
    55741 catgagtaga attttagata atgagataat gggggatgag gagttagtag aacgcacgct
    55801 ccgtcctcag tatttacgtg aatatatcgg acaggataag gtcaaggacc agctacaaat
    55861 ctttattgaa gctgccaaaa tgcgggatga agcgctggat catgtgctct tatttgggcc
    55921 tccaggtctc gggaaaacga ccatggcctt tgttattgcc aacgaactgg gagtcaatct
    55981 taagcagacg tcgggtccag tcattgaaaa agccggagat ctggtagcta ttttgaatga
    56041 gttagagcct ggggatgtac tttttattga tgagatccat cgtttgccaa tgtcagtgga
    56101 agaggtgctt tatagtgcta tggaggactt ctacatagat attatgattg gggctggtga
    56161 gggtagtcgt agtgttcatt tggagttacc accttttacc ttgattggtg cgacgactcg
    56221 ggctggtatg ctctccaatc cgctacgggc acgttttggg attacaggcc atatggagta
    56281 ttatgcccat gctgacttga cagaaattgt cgagcggacg gcagatattt ttgagatgga
    56341 aatcactcat gaggcagcat ctgagttggc cctacgtagt cgtgggaccc ctcgtattgc
    56401 caatcgtctc ctcaagcgcg tgcgcgattt tgcccagata atggggaatg gggtaattga
    56461 tgatattatt accgataagg ctttgactat gctggatgtt gaccatgaag gtttggacta
    56521 tgtggatcaa aaaatccttc gtaccatgat tgagatgtac agtggaggac ctgttggtct
    56581 aggaactctt tctgtgaata tcgccgaaga gcgtgagaca gttgaagata tgtatgagcc
    56641 ttacttgatt caaaaaggtt ttatcatgcg gacacggtct ggacgggtgg cgactgctaa
    56701 ggcatatgag cacttaggtt atgaatacag tgaaaaataa gcaagaaatc ttagaggctt
    56761 ttagagaaaa tccagatatg atggccattc tgacgatcat ccgagacctt ggtctgaaag
    56821 actcgtggtt ggcagcaggt tctgtcagaa atttcatttg gaatcttttg tcagacaaat
    56881 ccccttttga tcatgaaaca gatatagatg tgattttctt tgatccagat ttttcttatg
    56941 aggaaacctt attactggag aaaaagctgc gagaggattt tcctcagtac cagtgggaat
    57001 tgaaaaatca ggtctatatg catcagcaca gtcctcacac tgcttcctat accagttctc
    57061 gtgatgctat gagtaagtat ccagaacggt gtacgacagt tggactgcgc ttgaatgaag
    57121 aatcagattt tgaactctat gcaccttatg gtttggagga tattttgaat tttcaagttc
    57181 gtccaactcc tcatttctta gaaaatgaag accgaatgga actctatcaa acacgtttat
    57241 ccaagaaaaa ttggcaggag aaatggaaaa atttgatttt taaaaatact taaggaaact
    57301 ttaagctagg gagtgtacac taagttcata agttaagaag accttaactt aaactcctaa
    57361 aactttttca taataatctc cctataaaaa taaagtcgcc caatcaggcg gcttattttt
    57421 ttgaaaaatg ggcttggtgc ctgagaataa atagcttagt gatagaagaa aatggggaaa
    57481 tatggtataa taaaacgata gatttttgaa taggaataag atcatgtttg gattttttaa
    57541 gaaagataag gctgtggaag tagaggttcc gacacaggtt cctgctcata tcggcatcat
    57601 catggatggc aatggccgtt gggctaaaaa acgtatgcaa ccgcgagttt ttggacataa
    57661 ggcgggcatg gaagcattgc aaaccgtgac caaggcagcc aacaaactgg gcgtcaaggt
    57721 tattacggtc tatgcttttt ctacggaaaa ctggacccgt ccagatcagg aagtcaagtt
    57781 tatcatgaac ttgccagtag agttttatga taattatgtc ccggaactac atgcgaataa
    57841 tgttaagatt caaatgattg gggagacaga ccgcctgcct aagcaaacct ttgaagcttt
    57901 aaccaaggct gaggaattga ctaagaacaa cacaggattg attcttaatt ttgctcttaa
    57961 ctatggtgga cgtgctgaga ttacacaggc gcttaagttg atttcccagg atgttttaga
    58021 tgccaaaatc aatccaggtg acatcacaga ggaattgatt ggtaactatc tctttaccca
    58081 gcatttgcct aaggacttac gagacccaga cttgattatc cgtactagtg gagaattgcg
    58141 tttgagcaat ttccttccat ggcagggagc ctatagtgag ctttatttta cggacacctt
    58201 atggcctgat tttgacgaag cggccttgca ggaagctatt cttgcctata atcgtcgtca
    58261 tcgccgattt ggaggagttt aggaggaaat atgacccagg atttacagaa aagaaccttg
    58321 tttgcaggga ttgccctggc tattttccta ccaattttaa tgattggggg cctcttgctt
    58381 cagatagcaa tcggaatcat agccatgcta gccatgcatg aacttttgaa gatgagaggt
    58441 ctagagacca tgacgatgga gggcctcttg accctctttg caacctttgc attgaccatt
    58501 cccttggaga attacctgac ttttttgcca gttgatggga atgtggttgc ctatagtgtt
    58561 ttgatttcaa tcatgttagg aacgaccgtt tttagcaagt cttatacgat tgaggatgcg
    58621 gttttccctc ttgctatgag cttctacgtg ggctttggat ttaatgcttt actagatgct
    58681 cgtgttgcag gtttggacaa ggctctctta gccttgtgta tcgtctgggc gacagacagt
    58741 ggtgcctatc ttgttgggat gaactatggg aaacgaaagt tagcaccaag ggtatcgcct
    58801 aataaaaccc ttgagggtgc cttgggtggt attttaggag caattttagt aaccattatc
    58861 tttatgatag ttgacagtac agttgctctt ccatatggaa tttacaagat gtcagtcttt
    58921 gctattttct ttagcattgc tggacaattt ggtgatttac tagaaagttc gatcaaacgt
    58981 cattttggtg ttaaggattc tgggaaattt atccctggac atggtggtgt tttggatcgt
    59041 ttcgatagta tgttgcttgt atttccaatc atgcacttat ttggactctt ttaatcaaaa
    59101 gacggaggaa acgctatgct cggaatttta acctttattc tggttttcgg gattattgta
    59161 gtggtgcacg agttcgggca cttctacttt gccaagaaat cagggatttt agtacgtgaa
    59221 tttgccatcg gtatgggacc taaaattttt gctcacattg gcaaggatgg aacggcctat
    59281 accattcgaa tcttgcctct gggtggctat gtccgcatgg ccggttgggg tgatgataca
    59341 actgaaatca agacaggaac gcctgttagt ttgacacttg ctgatgatgg taaggttaaa
    59401 cgcatcaatc tctcaggtaa aaaattggat caaacagccc tccctatgca ggtgacccag
    59461 tttgattttg aagacaagct ctttatcaaa ggattggttc tggaagaaga aaaaacattt
    59521 gcagtggatc acgatgcaac ggttgtggaa gcagatggta ctgaggttcg gattgcacct
    59581 ttagatgttc aatatcaaaa tgcgactatc tggggcaaac tgattaccaa ttttgcaggt
    59641 cctatgaaca attttatctt aggtgtcgtt gttttttggg ttttaatctt tatgcagggt
    59701 ggtgtcagag atgttgatac caatcagttc catatcatgc cccaaggtgc cttggccaag
    59761 gtaggagtac cagaaacggc acaaattacc aagattggct cacatgaggt tagcaactgg
    59821 gaaagcttga tccaagctgt ggaaacagaa accaaagata agacggcacc gactttggat
    59881 gtgactattt ctgaaaaggg gagtgacaaa caagtcactg ttacacccaa agatagtcaa
    59941 ggtcgttacc ttctaggtgt tcaaccgggg gttaagtcag attttctatc catgtttgta
    60001 ggtggtttta caactgctgc tgactcagct ctccgaattc tctcagctct gaaaaatctg
    60061 attttccaac cagatttgaa caagttgggt ggacctgttg ctatctttaa ggcaagtagt
    60121 gatgctgcta aaaatggaat tgaaaatatc ttgtacttct tggcaatgat ttccatcaat
    60181 attgggattt ttaatcttat tccgattcca gccttggatg gtggtaagat tgtgctcaat
    60241 atcctagaag ccatccgccg caaaccattg aaacaagaaa ttgaaaccta tgtcaccttg
    60301 gccggagtgg tcatcatggt tgtcttgatg attgctgtga cttggaatga cattatgcga
    60361 ctctttttta gataatcgag gaatattatg aaacaaagta aaatgcctat cccaacgctt
    60421 cgcgaaatgc caagcgatgc tcaagttatc agccatgctc ttatgttgcg tgctggttat
    60481 gttcgccaag tttcagcagg tgtttattct tatctaccac ttgccaaccg tgtgattgaa
    60541 aaagctaaaa acatcatgcg ccaagaattc gaaaagattg gtgctgttga gatgttggct
    60601 ccagcccttc ttagtgcaga attgtggcgt gaatcaggtc gttacgaaac ctatggtgaa
    60661 gacctttaca aactaaaaaa ccgtgaaaaa tcagacttta tcttaggtcc aactcacgaa
    60721 gaaaccttta cagctattgt ccgtgattct gttaaatctt acaagcaatt gccactcaac
    60781 ctttatcaaa ttcagcccaa gtatcgtgat gaaaaacgcc cgcgtaatgg acttcttcgt
    60841 acacgtgagt ttatcatgaa ggatgcttat agtttccacg ctaactatga tagtttggat
    60901 agtgtttatg atgagtacaa agcagcctat gagcgtattt tcactcgtag tggtttagac
    60961 ttcaaggcta ttattggtga cggtggagcc atgggtggta aggatagcca agaatttatg
    61021 gccattacat ctgctcgtac agaccttgac cgctgggttg tcttggacaa gtcagttgcc
    61081 tcatttgacg aaattcctgc agaagtgcaa gaagaaatca aggcagaatt gctcaaatgg
    61141 atagtctctg gtgaagatac cattgcttac tcaagtgagt ctagctatgc agctaactta
    61201 gaaatggcaa caaacgagta caaaccaagc aaccgtgttg tcgctgaaga agaagttact
    61261 cgtgttgcaa cgccagatgt taaatcaatt gatgaagttg cagccttcct caatgttcca
    61321 gaagaacaaa cgattaaaac cctcttctac attgcagatg gtgagcttgt tgcagccctt
    61381 ctagttggaa atgaccaact caacgaagtc aagttgaaaa atcacttggg agcagatttc
    61441 tttgacgttg ctagcgaaga agaagtggcg aatgttgttc aagcaggatt tggttcactt
    61501 ggaccagttg gtttgccaga gaatattaaa attattgcag atcgtaaggt acaagatgtt
    61561 cgcaatgcag ttgtcggtgc taacgaagat ggctaccact tgactggtgt gaacccaggc
    61621 cgtgatttta ctgcagaata tgtggatatc cgtgaagttc gtgagggtga aatttcccca
    61681 gatggacaag gtgtccttaa ctttgcgcgt ggtattgaga tcggtcatat tttcaaactc
    61741 ggaactcgct attcagcaag catgggagca gatgtcttgg atgaaaatgg tcgtgctgtg
    61801 ccaatcatca tgggatgtta cggtatcggt gtcagccgtc ttctttcagc agtgatggag
    61861 caacacgctc gcctctttgt taacaaaacg ccaaaaggtg aataccgtta cgcttgggga
    61921 atcaatttcc ctaaagaatt ggcaccattt gatgtgcatt tgattactgt caatgtcaag
    61981 gatgaagaag cgcaagcctt gacagaaaaa cttgaagcaa gcttgatggg agctggttat
    62041 gaagtcttga cagatgaccg taacgaacgt gtcggtgtta aatttagcga tagtgacttg
    62101 attggtcttc caatccgtat cactgttggt aaaaaagcga ccgatggcat tgtagaagtt
    62161 aagatcaagg caactggtga caccattgag gttcatgcag ataacgtgct tgaaacgctt
    62221 gaaatcctaa gcaagaaata aaaactataa tcagaagaaa aacaaggaaa aaatgtaact
    62281 agtttttacc ttgttttttc tgtataatgg gagaaatgag tagataaaga ggtaaatgta
    62341 tgctaagatt tccaaaggat tttgtctggg gatcctctac ttctgggccg cagacagaag
    62401 gacgtgtagc tggtgacggt aagggagaca atctctggga ttactggttc caagtggagc
    62461 caaatcgtta ctataatggg attggttcag ataagacatc gactttttat gaaaattggg
    62521 agcgggatat tgagcttttg ttagagactg gtcacacagc ctttcggact tctattcagt
    62581 ggtcacggat ttttccacaa ggctgtggaa aagtcaacac tcaaggtgtg gatttttatc
    62641 gtaaggtctt tgaggctatt aaggctaaag gaattcgtct gttagtcaat ctctatcatt
    62701 ttgatttacc ttttgccctt caagaggatg gtgatggttg ggaaaataag gcgacagtct
    62761 cagcctatga agactatgct cgtttttgtt ttgagactta tggagattta gtggatcaat
    62821 ggattacctt taacgagccc atcgttcctg tagaatttgg ttatttttat gatgcccatt
    62881 atccacataa ggtggatgca gaggcagctg ttaaggtagc ctatcataca caattggcca
    62941 gcagccgggc tgttaaggct tgccatgaac ttttgcctga ttccaagatt gggattgtcc
    63001 tcaacttgac accggcttat ccacgtagcc agcatcctgc tgatgtcaag gcagctcgta
    63061 ttgcggccct ttttcaggcc caatctttct tagatccatc tgccttgggg acttatccac
    63121 aggagttggt agaaatcttg catgaacacg gtcttttacc tgatgctaca gaggaagagt
    63181 tggaactcat tcgtgataat acggtggact tccttggtgt gaactactat caacctttgc
    63241 gtgttatggc tcctcgattt gctaagcatc cagagagtcc actcttacca gaacattttt
    63301 acgagcctta tgtgatgcct ggacgtaaaa tcaattctca ccgtggttgg gagatttatg
    63361 agcaagggat ttatgacatc gcccaaaata tcaaggaaaa ttatggcaat attgagtgga
    63421 tgttgactga gaatggtatg ggtgttgaag gggaagaaaa attccgtcaa gatggaatga
    63481 ttcaagatga ttaccgtatt gactttgtaa aaggtcatct tcgtgaactt caccgtgcca
    63541 ttgaagatgg tgccaactgt aagggctact tgatttggac ctttattgac tgctggtcat
    63601 ggctcaacag ctataaaaat cgctatggtt tggtcgaatt agacttggaa acgcaaaaac
    63661 gtcgtctgaa gaaatcaggc cactggttca aagaattaag cgataataat ggattttaaa
    63721 aaggactctg actgattatc ccaatcggtc agagtttttt gcttacagga gcttaatttg
    63781 aactatacca atttttactc ttgacaagtg aaagaaacaa gtatatactg tttttgctga
    63841 ttctataaaa gatgaattag actataccaa ttttaaggag aaagcacagc ttgcctgtgt
    63901 cgtatatact atgtgtggaa ttgttggtgt tgttggaaac acaaatgcaa ctgatatttt
    63961 gattcaaggg cttgaaaagc ttgaataccg tggctatgat tctgcgggaa tttttgtcct
    64021 agatggtgct gataaccatt tggtgaaggc tgttggtcgt atcgcagaat tgtctgccaa
    64081 gacagctggt gttgagggaa caactggtat cggacatact cgttgggcaa ctcatgggaa
    64141 accaacggaa gacaatgctc acccacaccg ctctgagaca gaacgttttg tcttggtgca
    64201 taatggggtg attgagaact atcttgaaat caaggaagaa taccttgcag gtcaccactt
    64261 caaggggcag acagatactg aaatagccgt tcatttgatt ggaaaatttg cggaagaaga
    64321 agggctctca gttcttgaag ccttcaaaaa agcccttcac atcatccgtg gttcttatgc
    64381 ctttgccttg gttgactcac aagatcctga agtcatctac gtagctaaaa ataaatcacc
    64441 acttttgatt ggtcttggag aaggctacaa tatggtctgc tcagatgcta tggctatgat
    64501 tcgtgaaacc aaccaataca tggaaattca tgaccaagag ttggtaatcg tcaaggctga
    64561 tagcgtggaa gttcaagact atgatggtaa ccgtcgtgaa cgtgctagct atactgcgga
    64621 acttgacttg tcagatatcg gtaagggaac ttatccttac tacatgctta aggaaattga
    64681 tgagcaacca actgttatgc gtaaactcat tcaagcctac acggatgatg ctggtcaagt
    64741 agtggttgat cctgctatca ttaaggctgt tcaagacgca gaccgcatct acatccttgc
    64801 agctggaaca tcttaccatg caggatttgc ttctaagaaa atgttggaag aattgacaga
    64861 tacaccagtt gaacttggaa tctcatctga gtggggctac ggtatgccac ttctcagcaa
    64921 gaaaccactc ttcatcttta tcagccaatc tggtgaaaca gcggatagtc gtcaagtttt
    64981 ggtcaaggct aatgaaatgg gaattccaag cttaacagtg acaaatgttc caggttcaac
    65041 cctctcacgt gaagccaact ataccatgct ccttcacgca ggtcctgaaa ttgccgtggc
    65101 atcaactaaa gcctatacag cgcaaatcgc agcccttgcc ttccttgcaa aagcagtcgg
    65161 agaagcaaat ggtaatgcta aagcgcaagc ctttgacctg gttcatgaat tgtcaatcgt
    65221 agctcagtct atcgaatcaa ctctttcaga gaaagaaacc attgaagtca aggttcgtga
    65281 acttcttgaa acaactcgta acgcctttta catcggacgt ggtcaagatt actacgtagc
    65341 catggaagca agtctcaaac tcaaagagat ttcttatatc cagtgtgaag gctttgctgc
    65401 aggagaactc aagcacggaa ccattgcctt gattgaagaa ggaacgcctg ttttggctct
    65461 cttgtcagat ccagttcttg ccaatcatac tcgtggaaat atccaagagg tcgcagcccg
    65521 tggtgccaaa gtcctcacta tcgcagaaga gaatgtagcc aaagataccg acgatatcgt
    65581 ccttacgacc gtacatccat acctctcacc aatttcaatg gtcgtatcaa cgcaattagt
    65641 cgcttacttt gcaaccctcc accgtggcct cgatgtggac aaaccacgta accttgccaa
    65701 gtcagtaacg gtagaataag ctaaaaaagt ctagttatct aggctttttc taatgagcaa
    65761 attggttgct tgtactcaag attcgtgtta gaataatttt tcaaattgaa gtacagagat
    65821 agataaggag aatcacaatg gtagaattgg gaatttcaac atttggggaa ataacggagc
    65881 ttgaagggac tggacaaact tacagtcatg ctgaacgcat tcgccagttg gtggcagaga
    65941 ttgagctggc tgacaaggtt ggtttggatg tatatgggat tggtgagcac catcgggcgg
    66001 attttgcagt atcagcccca gagattgttc tggccgctgg ggcagtcaat accaagaaaa
    66061 tccgtttgac cagtgcagtc agcattctgt caagcatgga cccgattcgt ttgttccaac
    66121 agtatgccac tatcgatgct ttgtcaaatg gacgatcgga gattatggct ggaaggggct
    66181 ctttcacgga atctttccct ctgtttggtt atgacttgaa agactacgac tctctttttg
    66241 atgagaaatt agacttgctt cagttagtca atgaaaagac caagttagac tggcaaggtc
    66301 gattgactca aacgattgct ggtaaagaag tttatcctcg tccagttcag gacaaattgc
    66361 ccttgtggat agctacaggt ggtcatgtcg aatcaacagt gaagattgct caggctggtc
    66421 taccgattgt atatgctatt attggtggta atccacgtta ttttaaaaag ttgattcagg
    66481 cttatcgtga gattggaagc gaagcgggcc atgctgacaa agacttgaaa gtgggagccc
    66541 attcttgggg gtggattgcg gaagatggtg agcaggcggt gaaagattat ttccatccga
    66601 ctaagcaagt ggtggatgct atttccaaag accgtccgca ctggcaggaa ttgcgttatg
    66661 agcaatattt ggagcaggta gggccaaatg gcgccatgtt tgttggcaat ccagatcagg
    66721 tggccgaaaa attgattcgc atgatagagg atttaggatt ggaccgcttc atgcttcatc
    66781 taccgcttgg ttctatgcct catgaccagg ttctgagagc tattgaactc ttcggaacac
    66841 aagttgcacc caaagtacga gcttattttg ctatgaaaga ggcttaataa aaaatcctaa
    66901 tcaagtttat taggacaaca aatattgtac aaaaattaat cccccgagct ataatgcttg
    66961 ggggattttt ctataggagg gctagtttag ttctcttttt tattttttag taagaaaccg
    67021 agaccaagga gcgcaaggag gctgattcct gcaaatagga gtttgttttc gtttttgatt
    67081 cctgtatttg gaagttcagc ttgtttatct ggggttgcag gtatattttc cttgctagag
    67141 ttttctaccg attcgtttcg aaccgcttct tttacagatg atgcttgtgc ttcttgtgct
    67201 ggttgttcag cttgtgaatc agctgtagct tggctaggtt tattttccgc atcagctact
    67261 ttggcatctg gtttagtaga atcaggtcta gcttctggag ctgggtcttg atgtgcaggg
    67321 tgagaagctt cattttgatg ttcaggcttg gaaggggttg agtctggttt ctcttctgca
    67381 gttgactcat ggctagttcc attttgagag actcgaagaa cagtagctgt aagggcattc
    67441 aatttcaagc ctttttcagt ccattcaagt cctttcgggt tggcaattcc gactgatcct
    67501 gcttggtttt catctgccaa aacttccgca tttcttagat gtgcaaaggc agttcccaaa
    67561 ttaaattcgc gagctttttc atccgcattg acaaagactg cgtagatatc gccgtttgga
    67621 gcagtgattt ggtagccaat cactacatcc tctttttcca caccattttg gcctgggaca
    67681 gtgatgaggt ggacacggtc tttgatatct tggagactct taagtcggaa ggcatctgta
    67741 gattgacgaa gggcaatcaa acctttcata tagtcacggc tcttgacatt ttcaggataa
    67801 gctttaccat ctgtagcctt agtccagtca aacttgttga ctgcgtcgct agaatcgtaa
    67861 gaatcatgga tgaagtaagg atagtcaaat gggttgccgt ccttatcacg caacaagtga
    67921 gatttgtttg gaaccttatc ctctgctact ggagtcttgt aggctgggtc acggaattgt
    67981 ttagtacgtc catattcctg accggagtgg ataaatggag ttccttgagc tgtcaagacc
    68041 atgagatttc caagtcgtaa acgacggtgg atttcagcat agttctcagc cttgcttggg
    68101 tcttttttga tagactgggc aatgatgtca aagagggtca agttatcatg ggctgcgatg
    68161 tattggatga catctccagg gctgtcagct tcaaagttag ttggttgagc aatgagattt
    68221 ttaaagatgg tgttgacatc acgcttgcca cctgtgataa aggcaggttg accttcgttt
    68281 ggataaccag atttgaggtt gttacggatg tcatctgaaa agacagcgac agtatcggta
    68341 tgtttcatcc aatcttggtc agcagcttta gtaggcatgt tttcatcacc ggcataggtt
    68401 ctccaacctt caccaagcat gatgaggttt ggattgaggg cgcgtgcagc cttgtaagct
    68461 tcttcgatag aagcggcgtc atggtctccc atcatatcga agcggaagcc atccactttg
    68521 taggtatcaa ctaggtattt gatagagtca actaggaggc gtttggtcat atggtgggtt
    68581 gtccccaagc gtccaccacc aaagctagtt cgaggtgtgc catcggcatc cataaagtgg
    68641 tagtagtttg gctctaggtc ttcaaagata tcgactttgg ctgtgtggtt ataaacgaca
    68701 tctaggatag ctcccatacc acgtttgtgg atttcgttga tgaggttttt aaattctgcg
    68761 attcgttttt ctggattctt aggatcgctt gagtacatac cagtcaagga gaagtagttt
    68821 tgagggtcat atccccagtt gtagttgctg ttgcttgaag catagtcaga caagcgttca
    68881 tggtttttca attcattgac aaagtagtaa gacaagactg gaaggagctg gatatgggtt
    68941 acacccaagt ctttgagata gtctagtttt tcaatgaagg cttcaaaagt cccaaatggt
    69001 ttggtcaagt cttttgcaat ggcaggatct gaagtgaagt cacgcacatg agcttcgtag
    69061 ataacggcgt cttcacgagt cttgaaattg tgaatcttac cataagtcaa gtcttgaggt
    69121 ccgagtttag ctggatctac aaaggcggct ttagccactt tatgggcatc gtcaatcttg
    69181 gcatcgttgc tattccaagc agcaagagat ttagcgtaag gatcgagtgc aagaacagtt
    69241 ttaccttgac gctcgatttg gtattgataa tagtagccag tgaaatctgt gattccgagt
    69301 ttgtttgtgc tgtctagagt ttgtttccaa gttcctcttt cccctttttc gagagcgaca
    69361 gttccaacta ctttgtcagg gtcattcttg tcgtagacaa caacagaaac cttatcagca
    69421 cttggtgacc aaagggtcaa atcaacttgt tttccttctt cttttaggtc agctcccagt
    69481 ttgccatcat agctgtatgt ctcatctttc aggcgccagc ttgttttggt agtgaattgg
    69541 tcggaattgt agctaacagt ataaggatgt tttgtgtcag agaaatctcc gctgtaggtc
    69601 actttcttac cagcttcatc gattgcaaca tcggtaatag ttaccttgtt tcctaggtga
    69661 ttagtgatgt tggagtgttt gaggatatct tcttttttag caccgacaag tgttgaaaag
    69721 ctactttcaa tgctagaagt gcctacgtgt tgggctcctg tcatacggat atcatggaca
    69781 tagtatggat ttgtgtaaat cgattcatca tcgtctttta ggaaaatttg gctatgattt
    69841 ttcaaatctg tgaacttata attttcttta cggattttca cgtcgtctcc ttgtttgctc
    69901 tcatctagta ataaaaatcc aaattctctt gcggcttcat taagaggaat gtcgatatag
    69961 cggccatatt tgcctgtagc cgtaaagtct gttccgtcag gccattgagc gctacttgga
    70021 tttttcacat ctccccagta ccagagagat ttcttgtcat agttgccatc tgtgcggtag
    70081 tagttgacgc gaacagttcc tgcaggctgt ggctcgtaag agaaaacctt gtaatcttgg
    70141 tctaaccaag cttcgttcat ttttggaact agtttttcta cagatttatc gccggttaga
    70201 ttttttccag ctgtattgtt gatgaggaag ctaattttct tggcttgttc tccctttaat
    70261 ttgacatcta ggtaatagcc gtagtcatct ttcttggcat ccttgaagga caaagctccg
    70321 tttggccagt tttcagatgg tttttcaaca tcgtcccaag tccatagtcc ttgagcatcc
    70381 ttgttttctt caggaagttt tttgacatgg atacggaagt agttatcttc gatgggctct
    70441 tctgccttag tttcagttgt tactggacta gtagaagtag ttggctcact tgaacgatct
    70501 tgtccagttt gtggtgctgg atcggctggg cttgcagcag gagttggtgg ctctgttgca
    70561 ggtttttctt ctgttttctc tagggaagcg tttgcatttc caggtgaaga aatatcactt
    70621 tcgttcttac cagagccagc tgctgtatca gtaagaggtt gggcaagggt ggttgtattt
    70681 ctgttgttgg gttcgttagt ggttggtgta ctttcgtcgg atgagatagt tggtgtagcc
    70741 atggcaagca agacaaggct tgcgccgata aggacagaac cagtcccatt tttgagggaa
    70801 cggatgctgt agaccatttt tttctcagtg tgagatggtg tttttctcat aatgattctc
    70861 caatcaataa atttctatat cagtatagca tgtagtaaaa caatatgcaa gcgttttctt
    70921 tgatttgtga aaaaaattct tacttttgag aagttttctg tactatttta tatatcagta
    70981 taaatgacgg agataaaaat gtcacataag ccaagccgta ttattaggaa gatgatgtgt
    71041 tcccagctat ttttattcag aaattgatct ctaacattac caataaaaaa gaaaaatatc
    71101 ttgacaagca agggaaaatt ttgttacaat aatagacggt actttttact tttggtctct
    71161 caagagtgta cagggacgtg ctgacaaatg ttgcaaaagt acacacagat gatatctgtc
    71221 accaagtgta tcatcaccaa aaataaaaaa acacaggaga atgtagatgc ctacaattaa
    71281 ccaattggtt cgcaaaccgc gtaaatcaaa agtagaaaaa tctaaatcac cagctttgaa
    71341 cgttggttac aatagtcata aaaaagttca aacaaacgtt tcttcaccac aaaaacgtgg
    71401 tgttgcaact cgtgttggaa caatgacacc taaaaaacct aactcagccc ttcgtaaatt
    71461 cgctcgtgta cgtttgagca accttatcga agttactgcc tacatcccag gtatcggaca
    71521 caacttgcaa gagcacagcg tggtgcttct tcgcggtgga cgtgtaaaag accttccagg
    71581 ggtacgttat catatcgtcc gtggtgcact tgatactgca ggtgttaacg atcgtaaaca
    71641 aggccgttct aaatacggta ctaaacgtcc aaaagcataa ggaaagggga taaagagaaa
    71701 tgagtcgtaa aaatagagct ccaaaacgtg acgtattgcc agatccgcta tacaattcac
    71761 aactagttac tcgtcttatc aaccgcgtta tgcttgatgg taaacgtggt actgctgctt
    71821 caatcgttta cggtgctttt gagcaaatca aagaagctac tggcaacgat gcacttgaag
    71881 tatttgaaac agctatggaa aacatcatgc ctgtacttga agtacgtgca cgtcgtgttg
    71941 gtggttctaa ctaccaagtc ccagttgaag ttcgtccaga acgtcgtaca acacttggac
    72001 ttcgttggtt ggtaacaatc gctcgtcttc gtggtgaaca cacaatgcaa gaccgtcttg
    72061 caaaagaaat cttggatgct gctaacaaca ctggtgcagc agttaagaaa cgtgaagata
    72121 ctcaccgtat ggctgaagct aaccgtgcat tcgcacactt ccgttggtaa gataggatgc
    72181 gaaagcgtta agaaagtccc agagaaaata gggaatcgaa gcaggttgcg gttgcaacca
    72241 atgagattca tctttttctc cagactttta gcttgagctc aactaaatca tgatgctagg
    72301 cacggtaagg atgcaaggtg aaaataggaa actgacgcag tattcaacga atacaaggag
    72361 ttttatcttt ttcacgcagc atcccgttcc agctcacatc ggctaactaa ctttagcctg
    72421 ggttcaaatt agctaaatcg attagtatta gctataactc agcttaccat ctcgtaagtt
    72481 gaaaccaaca atagcatgaa aacattgaga acgggtaggt cctgcctatc cgtttttatt
    72541 aaaatcgtgt tataatagaa tagaaatcaa aaataaatag gagaaacaaa cctcatggca
    72601 cgcgaatttt cacttgaaaa aactcgtaat atcggtatca tggctcacgt cgatgccggt
    72661 aaaacaacaa ctactgagcg tattctttac tacactggta aaatccacaa aatcggtgaa
    72721 actcacgaag gtgcgtcaca aatggactgg atggagcaag agcaagaacg tggtatcacg
    72781 atcacatctg ctgcgacaac agctcaatgg aacaaccacc gcgtaaacat catcgacaca
    72841 ccaggacacg tggacttcac aatcgaagta caacgttctc ttcgtgtatt ggatggtgcg
    72901 gttaccgttc ttgactcaca atcaggtgtt gagcctcaaa ctgaaacagt ttggcgtcaa
    72961 gcaactgagt acggagttcc acgtatcgta tttgccaaca aaatggacaa aatcggtgct
    73021 gacttccttt actctgtaag cacacttcac gatcgccttc aagcaaatgc acacccaatc
    73081 caattgccaa tcggttctga agatgacttc cgtggtatca ttgacttgat caagatgaaa
    73141 gctgaaatct atactaacga ccttggtacg gatatccttg aagaagacat cccagctgaa
    73201 taccttgacc aagctcaaga ataccgtgaa aaattgattg aagcagttgc tgaaactgac
    73261 gaagaattga tgatgaaata cctcgaaggt gaagaaatca ctaacgaaga attgaaagct
    73321 ggtatccgta aagcgactat caacgttgaa ttcttcccag tattgtgtgg ttcagccttc
    73381 aaaaacaaag gtgttcaatt gatgcttgat gcggttatcg actaccttcc aagtccactt
    73441 gacatcccag caatcaaagg tattaaccca gatacagacg ctgaagaaac tcgtccagca
    73501 tctgacgaag agccatttgc agctcttgcc ttcaagatca tgactgaccc attcgtaggt
    73561 cgtttgacat tcttccgtgt ttactcaggt gttcttcaat caggttcata cgtattgaat
    73621 acttctaaag gtaaacgtga acgtatcgga cgtatccttc aaatgcacgc taacagccgt
    73681 caagaaatcg acactgttta ctcaggtgat atcgctgctg ccgttggttt gaaagatact
    73741 acaactggtg actcattgac agatgaaaaa gctaaaatca tccttgagtc aatcaacgtt
    73801 ccagaaccag ttatccaatt gatggttgag ccaaaatcta aagctgacca agacaagatg
    73861 ggtatcgccc ttcaaaaatt ggctgaagaa gatccaacat tccgcgttga aacaaacgtt
    73921 gaaactggtg aaacagttat ctcaggtatg ggtgaacttc accttgacgt ccttgttgat
    73981 cgtatgcgtc gtgagttcaa agttgaagcg aacgtaggtg ctcctcaagt atcttaccgt
    74041 gaaacattcc gcgcttctac ccaagcacgt ggattcttca aacgtcagtc tggtggtaaa
    74101 ggtcaattcg gtgatgtgtg gattgaattt actccaaacg aagaaggtaa aggattcgaa
    74161 ttcgaaaacg caatcgtcgg tggtgtggtt cctcgtgaat ttatcccagc ggttgaaaaa
    74221 ggtttggtag aatctatggc taacggtgtt cttgcaggtt acccaatggt tgacgttaaa
    74281 gctaagcttt atgatggttc atatcacgat gtcgactcat ctgaaactgc cttcaagatt
    74341 gcggcttcac tttcccttaa agaagctgct aaatcagcac aaccagctat ccttgaacca
    74401 atgatgcttg taacaatcac tgttccagaa gaaaaccttg gtgatgttat gggtcacgta
    74461 actgctcgtc gtggacgtgt agatggtatg gaagcacacg gtaacagcca aatcgttcgt
    74521 gcttacgttc cacttgctga aatgttcggt tacgcaacag ttcttcgttc tgcatctcaa
    74581 ggacgtggta cattcatgat ggtatttgac cactacgaag atgtacctaa gtcagtacaa
    74641 gaagaaatta ttaagaaaaa taaaggtgaa gactaatccg tcctcactct agaaggaagt
    74701 cacttagtgg cttccttttg tctttagaaa atacctctaa atatggtaaa atagtagaag
    74761 aataatgtga ggaaaatgaa tgtcaaatag ttttgaaatt ttgatgaatc aattggggat
    74821 gcctgctgaa atgagacagg ctcctgcttt agcacaggcc aatattgagc gagttgtggt
    74881 tcataaaatt agtaaggtat gggagtttca tttcgtattt tctaatattt taccgattga
    74941 aatcttttta gaattaaaga aaggtttgag cgaagaattt tctaagacag gcaataaagc
    75001 tgtttttgaa attaaggctc ggtctcaaga attttcaaat cagctcttgc agtcctacta
    75061 tagggaggct ttctctgaag gtccatgtgc tagtcaaggt tttaagtccc tttatcaaaa
    75121 tttgcaagtt cgtgctgagg gcaatcagct atttattgaa ggatctgaag cgattgataa
    75181 ggaacatttt aagaagaatc atcttcctaa tttagccaaa caacttgaaa agtttggttt
    75241 tccaactttt aactgtcaag tcgataagaa tgatgtcctg acccaagagc aggaagaggc
    75301 ctttcatgct gaaaatgagc agattgttca agctgccaat gaggaagcgc tccgtgctat
    75361 ggaacaactg gagcagatgg cacctcctcc agcggaagag aaaccagtct ttgattttca
    75421 agcgaaaaaa gctgcagcta aacccaagct ggataaggcg gagattactc ctatgatcga
    75481 agtgacgaca gaggaaaatc gtctggtatt tgaaggggtt gtttttgatg tggagcaaaa
    75541 agtgactaga acaggtcgtg ttttaatcaa ctttaaaatg acggactaca cttcaagttt
    75601 ttctatgcaa aagtgggtta aaaacgagga agaggcccag aagtttgatc tcatcaagaa
    75661 gaattcttgg ctccgagttc gagggaatgt ggagatgaat aacttcacac gcgatttgac
    75721 tatgaacgta caggatctgc aggaagttgt tcactatgag cggaaggatt tgatgccaga
    75781 aggtgagcgt cgggttgagt ttcatgctca tactaacatg tcgactatgg atgctttgcc
    75841 agaggtcgaa gagattgttg caacagctgc taagtgggga cacaaggcgg ttgctatcac
    75901 ggaccatggg aatgtccagt cctttccaca tggctataag gcggctaaga aagcgggaat
    75961 ccagctgatc tatggaatgg aagccaatat cgtggaggac cgtgtcccta tcgtctataa
    76021 cgaagtggag atggacttgt cagaagcaac ctacgtggtc tttgacgtgg aaacgacggg
    76081 actttcagct atctataatg acttgattca ggttgcggcc tctaagatgt acaaggggaa
    76141 tgttattgct gaatttgatg aatttatcaa tcctgggcat cccttgtcag cttttactac
    76201 agagttaact ggaattacag atgatcatgt caaaaatgcc aaaccactag aacaagtttt
    76261 gcaagaattc caagaatttt gcaaggatac ggtcctagtt gcccacaatg ctacctttga
    76321 cgttggcttt atgaatgcta attatgagcg tcatgatctt ccaaagatta gtcagccagt
    76381 tattgatacg ctggagtttg ctagaaacct ctatcctgag tataaacgcc atggtttggg
    76441 gcctttgacc aagcgttttg gtgtggcctt ggaacatcac cacatggcca actacgatgc
    76501 ggaagcgact ggtcgtctgc ttttcatctt tatcaaagag gtagcagaaa aacatggtgt
    76561 gaccgattta gctagactca acattgatct aatcagtcca gattcttaca aaaaagctcg
    76621 gatcaagcat gcgaccatct atgtcaagaa tcaggtaggt ctaaaaaata tctttaagct
    76681 ggtttccttg tctaatacca agtattttga aggagtgcca cggattccga gaacggttct
    76741 agatgcccat cgagagggct tgattttagg ttcagcctgt tcagagggtg aagtttttga
    76801 cgtggtcgtt tctcaaggtg tggatgcggc ggttgaggtg gccaagtatt atgactttat
    76861 cgaggtcatg ccaccggcta tctatgcgcc cttgattgct aaagagcagg tcaaggatat
    76921 ggaggaactc cagaccatta tcaagagttt gatagaggtt ggagaccgcc ttggcaagcc
    76981 tgttttggct acgggaaatg ttcactatat cgaaccggaa gaagagattt atcgtgaaat
    77041 tatcgtccgt agtttgggac agggtgcgat gattaaccga actatcggtc atggtgaaca
    77101 tgcccaacca gcaccacttc caaaggctca ttttcgaacg actaatgaga tgttggatga
    77161 atttgccttt ttgggagagg aactggctcg taaactggtt attgaaaaca ccaatgcctt
    77221 ggcagaaata tttgaacccg ttgaagtcgt taagggtgac ttgtatacgc ctttcgtcga
    77281 caaggctgaa gaaacagttg ctgagttgac ctataagaaa gcttttgaga tttatggaaa
    77341 tccgctgcca gatattgttg atttgcggat tgaaaaagaa ttaacatcca tactggggaa
    77401 tggatttgct gtgatttatc tggcatcgca gatgctggtg caacgttcta atgaacgggg
    77461 ttatttggtt ggttctcgtg ggtctgtcgg atctagtttc gttgcgacca tgattgggat
    77521 tacggaggtc aatcctctct ctcctcacta tgtctgtggt cagtgtcagt acagtgagtt
    77581 tatcacagat ggttcgtacg gttcaggatt tgatatgccc cataaggact gtccaaactg
    77641 tggtcacaaa ctcagtaaaa acggacagga tattccgttt gagaccttcc ttggttttga
    77701 tggggataag gttcctgata ttgacttgaa cttctcggga gaagatcagc ctagcgccca
    77761 cttggatgtg cgtgatatct ttggtgaaga atatgccttc cgtgcgggaa cggttggtac
    77821 ggtagctgcc aagactgcct atggatttgt caagggttac gagcgagatt atggcaagtt
    77881 ttatcgtgat gcagaagtag aacgcctcgc tcaaggagcg gcgggtgtca agcggacaac
    77941 aggccaacac ccggggggaa tcgttgttat tccgaactac atggatgtct acgattttac
    78001 gcctgtccag tatccagcag atgatgtcac ggctgaatgg cagaccactc actttaactt
    78061 ccacgatatc gatgagaacg tcctcaaact cgatgtactg ggacatgatg atccgaccat
    78121 gattcgaaaa cttcaggatt tgtctggtat tgaccctaat aaaattccta tggatgacga
    78181 aggcgtgatg gcactctttt ctgggactga tgtgctaggg gtaacacctg aacaaattgg
    78241 aacgcctacg ggtatgttgg ggattccaga gtttggaaca aatttcgtac gtggaatggt
    78301 agacgaaacc catccgacaa cctttgcgga attgcttcag ctgtctggtc tgtcccacgg
    78361 tactgacgtt tggttgggga atgctcagga tctgattaag caaggaatag cggacctatc
    78421 gactgttatc ggttgtcggg acgacatcat ggtttacctc atgcatgcgg gtctggaacc
    78481 taagatggcc tttaccatta tggaacgggt acgtaagggt ttgtggctaa agatttcaga
    78541 agaggagaga aatggctata tcgaagcaat gaaggctaat aaggtgccag agtggtatat
    78601 cgaatcctgt gggaaaatta agtacatgtt ccctaaggcc catgcggcag cctacgttat
    78661 gatggccttg cgtgtagctt acttcaaggt tcaccatcct atttattact actgtgctta
    78721 cttctccatt cgtgctaagg cttttgatat caagaccatg ggtgcgggct tggaggtcat
    78781 caagcgcaga atggaagaaa tctctgaaaa acggaagaac aatgaagcct ctaatgtgga
    78841 aatcgatctc tatacaactc ttgagattgt caatgagatg tgggaacgag gtttcaagtt
    78901 tggtaaatta gatctctact gtagtcaggc gacagagttc ctcatcgacg gggataccct
    78961 tatcccacca tttgtagcaa tggatggtct gggagagaac gttgccaagc aactggtgcg
    79021 ggcgcgtgaa gagggagaat tcctctctaa aacagaacta cgcaagcgtg gtggactctc
    79081 atcaaccttg gttgaaaaga tggatgagat gggtattctt ggaaatatgc cagaggataa
    79141 ccagttgagt ttgtttgatg agttgtttta aaaaattgct taataatcta ttaaaagagg
    79201 ctaacgtata tccaatagat ttacattagc tttctttttt gttaaaatag tctatggaaa
    79261 gagggtgaga gtatgtcaaa gatgagtata agcatccgtc tggatagtga ggttaaggag
    79321 caggcccaac aggtgtttag taatctggga atggatatga caacagctat taatattttc
    79381 cttcgtcagg caattcaata tcagggacta ccttttgatg ttagactaga cgaaaatcgg
    79441 aagttgctcc aagcgttaac ggatttagac caaaatcgta atatgagcca gtcttttgaa
    79501 tcagtctcag atttgatgga ggacttacgt gcttaagatt cgttatcata aacagtttaa
    79561 aaaagatttt aagttggcta tgaagcgtgg tttgaaggca gaattattag aagaagtttt
    79621 gaattttctg gttcaagaaa aagaacatcc tgccagattg gcttttggtt tataaagtag
    79681 acaagtcgga attgatttta aatttgctga ggacaggcag tcacagtgat ttattttaat
    79741 ctattttaag ggggttctca tgaaactaag aatatttgcg gaagataagc cggctaagaa
    79801 ggtatttgaa tatcaattag aacttgctga tcgtacaatt cttctatcga cagcactctt
    79861 gtcaggtgct attgctttag caggaatctt ttctgctttg aaagaaaaat aaaaatagaa
    79921 aagagaaaac aggatggttt taccaaattt taaagaaaat ctagaaaaat atgcgaaatt
    79981 gttggttgcg aacggaatta acgtgcaacc tggtcacact ttggctctct ctattgatgt
    80041 ggagcaacgt gaattggcac atctaatcgt gaaagaagct tatgccttgg gtgcgcatga
    80101 ggtcatcgtt cagtggacag atgatgtgat taaccgtgag aaattcctcc atgccccgat
    80161 ggagcgtttg gacaatgtgc cagaatacaa gattgctgag atgaactatc tcttggagaa
    80221 caaggctagc cgtcttgggg ttcgttcatc tgatccaggt gccttgaacg gagtggatgc
    80281 tgacaagctt tcagcttctg ctaaagctat gggacttgcc atgaagccta tgcgtatcgc
    80341 aactcaatct aacaaggtta gctggactgt agcagctgca gcaggacttg agtgggctaa
    80401 gaaagtcttc ccaaatgctg cgagcgacga agaagcagtt gatttccttt gggaccaaat
    80461 tttcaaaact tgccgtgtct acgaagcaga tcctgttaag gcttgggagg aacatgcagc
    80521 cattctcaag agcaaggccg atatgcttaa taaggagcaa ttttcagccc ttcactacac
    80581 agcgccagga acagatttaa cacttggttt gccaaagaac cacgtttggg aatcagctgg
    80641 tgctgtcaat gcacagggcg aagaattctt gccaaatatg ccaacagaag aggtcttcac
    80701 agcgcctgac ttccgtcgtg cagatggtta tgtcacttct acaaaaccgc ttagctacaa
    80761 cggaaatatc attgaaggca ttaaggtgac ctttaaggat ggacaaatcg tagatatcac
    80821 tgctgagaag ggtgatcagg ttatgaaaga ccttgtcttt gaaaatgcgg gtgcgcgtgc
    80881 cttgggtgaa tgtgccttgg taccagatcc aagtccaatt tctcagtcag gcattacctt
    80941 ctttaacacc cttttcgatg aaaatgcgtc aaaccacttg gctatcggtg cagcctatgc
    81001 gactagcgtt gttgatggag cggagatgag cgaagaggag cttgaagctg cagggcttaa
    81061 ccgttcagat gttcacgtag actttatgat tggttctaac caaatggata tcgatggtat
    81121 tcgtgaggat ggaacgcgag tacctctttt ccgtaatggg aattgggcaa attaaggaga
    81181 taatatgtta ggaagtatgt tcgttggtct cctagtggga tttttagcag gtgctatgac
    81241 caatcgtgga gagcgaatgg gatgttttgg aaaaatgttt ctcggttgga tcggagcctt
    81301 tctaggtcac ttgctctttg gaacttgggg gccagtttta tcaggaacag ctattatccc
    81361 agcgatttta ggagccatga ttgttttagc tattttttgg agacgaggaa gttaatttcc
    81421 taaatctgat actcaatgaa aatcaaagag caaactagga aactagccgc aggttgctca
    81481 aagcactgct ttgaggttgc agatagaact gacgaagtca gtaacatata tacggcaagg
    81541 cgacgttgac gcggtttgaa gagatttttg aagagtatga aacgaaaagg aggttggtca
    81601 ttgtaccagc ctccttttga tatgatataa tagttctatg agattagata aatttttagt
    81661 tgcctgtgcg gtgggaagtc ggactgaggt caaaaacttg ctcaaggctg ggcgcgtgac
    81721 tgtaaatggt aaaaaagaaa aatcagctaa attgcagatt gatgaaaaaa tagatgagat
    81781 tcgctttgat gggcaagtgt tggagtatga agagtttgtc tactacatga tgaacaagcc
    81841 caaaggagtt atctcagcga ctgaggatcc caagcacaga accgttctgg acttgttgga
    81901 tgacttggcg cggagtaagg aagttttccc agtaggacgc ttggatattg acacgcatgg
    81961 tcttttgctc ttgaccaatg atggtcagct ggctcatgtt cttctttcgc ccaagcgtca
    82021 tgtggacaag acttatctgg cacaagtcaa gggaatcatg acccaagaag atgtggagac
    82081 atttgctgag ggtattcctc tcaaagactt tacctgtcaa cccgctatac tggagcttgt
    82141 atccatagat acagaaaaga atcaaagcca aatccgtgtg accattgcag aagggaagtt
    82201 tcatcagatc aagcgtatgg tgggctactg tggcaaggaa gtggtggact tgcaacgatt
    82261 gactatggga actttagtat tagatgagaa cctggaacga ggggaatggc gtcgcttgac
    82321 caaggaagaa ttagaaattc tccgcgctaa tattatttaa tttagtttaa tactcaatga
    82381 aaatcaaaga gcaaactagg aaactagccg caggctgtac ttgagtacgg caaggcgacg
    82441 ttgacgtggt ttgaagagat tttcgaagag tataaattcg aaaaaggtga gggacaatgt
    82501 ccttaccttt tatgtttttt agttgcttcc tttgtgaaaa gagttataat agactgtaga
    82561 ataaaaggag gaatctatga acgcgattca agaatcattt actaataaac tatttgccaa
    82621 ctatgaagca aatgtaaaat accaagcgat tgaaaatgct gccagccaca acggaatttt
    82681 tgcagctctg gaacgtcgcc aaagccatgt agacaacaca cctgttttct cattggattt
    82741 aaccaaggac aaggtcacta accagaaagc gtctggtcgt tgctggatgt ttgcggctct
    82801 caacaccttc cgccacaaac tcatctcgca atacaaattg gagaactttg agttgtcaca
    82861 agcccacact ttcttctggg ataagtatga gaaatcaaac tggttcttgg agcaagtcat
    82921 tgcgacttca gaccaagaat tgactagccg caaggttagc ttcttactcc aaacacctca
    82981 acaagatggt ggtcaatggg atatggtcgt ttccctcttt gaaaaatacg gtgtcgtgcc
    83041 taagtcagtt tatcctgagt ctgtttcatc tagcagcagt cgtgagctaa atgcgatcct
    83101 taataaattg cttcgtcaag atgctcaaat cttgcgtgat ttgcttgttt ctggtgcaga
    83161 tcaagtgact gttcaagcta agaaagaaga cctcttgcaa gaaatcttta actttcttgc
    83221 tatgtcatta ggacttccac cacgcaagtt tgactttgct tatcgcgata aagataacaa
    83281 ctacaaaagt gaaaaaggaa tcacaccaca agagttctac aagaaatatg tcaatcttcc
    83341 tttagaagac tacgtttctg ttatcaatgc tccaactgct gataaacctt acggaaaatc
    83401 ttacacagtt gagatgttgg ggaatgtggt tggtagccgt gcagttcgct acatcaacgt
    83461 tcctatggag cgcttgaaag agttggcgat tgctcaaatg caagcaggtg agactgtttg
    83521 gtttggttca gatgtcggtc agctcagcaa ccgtaaagct ggaatccttg cgacagatgt
    83581 ttatgacttt gaatcaagca tggacattaa acttactcaa gacaaggctg gacgtttgga
    83641 ttacagcgaa agcttgatga ctcacgccat ggtcttgaca ggtgttgatt tggatgaaaa
    83701 tggcaagtca atcaagtgga aggttgaaaa ctcatgggga gacaaggttg gtaccgatgg
    83761 ttactttgtt gcctcagacg cttggatgga cgaatacaca taccaaatcg ttgttcgtaa
    83821 ggaattgctg acagcagaag aacaagctgc ctatggagca gaaccaatcg tacttgcacc
    83881 atgggatcca atgggagcct tggctgaata aaagcataga aaaaaggaat cagattttag
    83941 aacctggttc ctttttagtt gcttgattac atgatgtgaa gaacatgtgc cacaataccc
    84001 actgcgaaga gggcaaggat aatagtgatt ggagatactt ttttcttaag taagtacatg
    84061 caaagtaaag taaggagtag tcctgataat ccaggaatca acatatccaa gttttgttgg
    84121 aaagtagtaa ctttttcagg agtttgagac aatccttgtc ctacttgtgc gaatgcttct
    84181 tggatacctt tagacccttc tggcaattta tcccaatgga tataagcctt ttcatctagt
    84241 tgaactttag aaacatcgaa agcaaattta atatttaccc agcgttgaac aaggacagca
    84301 agaatgaaca tcccaaggat agaagctcct ttagtgatat cttgaaggat accaccagac
    84361 atatctttag tgatttctga tccagccttg tatccaatct cttgaacata ccacaagaat
    84421 gacatacgaa tcaagttcca tgcaacaaag aagaggagtg gccccaagat attgccagta
    84481 agggcaagtg aagcaccgag agatccaagg attgggcgta ctgtaaacca gaatactggg
    84541 tcaccgatac cagcaagagg tcccatcata ccgattttaa ccccttggat agcagcgtca
    84601 tcgatttcca caccgttagc acgttcttct tcaagcgcaa gagtaacccc catgactgga
    84661 gcagctacgt atggatgagt gttgaagaac tcaaggtgac gctcaagagc agcgatttga
    84721 tcttcttttt tagtatagag ttttttgata gctggaatga gtgtataagc ccagcccaag
    84781 ttttgcatcc gttcaaagtt ccaagaccct tgtaagaagg ttgaacgcca ccaaactttt
    84841 ttacgatctg atttagttaa ttgaagtttt tcagtcatga tgttttcagt cctttcttat
    84901 cttagtagtc ttctaggata tcgccgattg ggtcgttaga agttgcggct cctccgccac
    84961 catttccacc agttttagaa aggtgaaggt agataagagc gatagcaaca ccgatagcac
    85021 cgaatccgat tagagtaata tctgacacag cagcgagaac gaaaccaaga gcgaagaatg
    85081 gccatacttc acgagttgcc atcatgttga taaccatggc gtaaccaacg gcaacgacca
    85141 taccaccacc gatagccata ccatctttga gccagtctgg catggcacta aggatacttt
    85201 gtacagtttc agttggtacc ataaggagaa gagctgcagg aagcgcgata cgaagtcctt
    85261 ggaaaagtag cgcaatgaaa tgcgcacgct ccacagcgcc gaagtcacct tttttagcgg
    85321 cagcatctgc agtatgaacc aaaccaactg aaattgtacg aacaatcatt gtcaagaaaa
    85381 gtccagctac ggcaagaggg atagcaaccg cttgggcaac accgatacca gtcttggtaa
    85441 agtcaccacc aagaaccatg ataatggcag cagcgacaga agcaagtgca gcatcaggag
    85501 cgatagcagc accgatattt gaccaaccaa gggcaatcat ttgaagcgat ccaccgagga
    85561 taatccctgc ttccaagtga cctgttacaa gcccaataag ggtacaggct acaagtggtt
    85621 ggtgaaattg gaactggtcg aggatgcctt caagacctgc aaagaaggct acaacgacta
    85681 ctaaaaccat agaaataata gacatgttta aaatcctttc ataaataatg gcttatttga
    85741 cattggcttt gttaatcaag tcaaacaaat cttttttaga atcatttggt actttacgga
    85801 catcaaattc aacacccaag tcacgcattt tttcaaatgt agcaacatct tctttgtcca
    85861 tagacaaaac ggtattgacc aatgttttac ctgttgagtg agccatagaa ccaacgttaa
    85921 gagtcttgat tggcacgccg ccttcgatgg cacgaagggc atcttgaggt gtttcaaaca
    85981 agataagggc atgtgtttct ccaaaacgtg ggtcttttga aatctcaatc agtttttgaa
    86041 ttggaaccac gttagccttg acattacctg gagctgcttg tttaatcaat tctttacgaa
    86101 ggtcgtcttt agccacgtta tctgaagcaa cgatgatacg atttgctttt gaatctggag
    86161 tccaagcagt tgcaacctga ccgtgaagta gacgtgtgtc aagacgggca agattgattt
    86221 tcaatttacc gtctccgata acagttcctt ctgggatagc agtttgggca actggagcag
    86281 ctgcagcgct tgcaacttct tcaactggat ttagctcttc tggaagagct ttgatgccat
    86341 ctttggcttc tttaatgata ttagcagcga ctttttctac acctgcagca gcgtccatga
    86401 ggcgctctgt gtaggcttga atcaacatcg gtaagttaag tcctgtgatg atggcaaact
    86461 tacgctcagg attttctccc atcacgcgac tagcttggtt aaatggagaa ccactccaaa
    86521 ggtcagccaa aactagaacc tcatcttctg cgtcaaatgc agcaacagcg ttattaaact
    86581 tagcgtatag atcatcagga ccttcatttg gcataaaggt cacaacttga accttttctt
    86641 gttcaccaaa gatcatagat cctgactgat gaatacccgc agcaaattcg ccgtggctcg
    86701 caataatgat tccgatactc attattgtca ttcctccttt tttgttttgg ttttcattta
    86761 agaaaacttt aagactgttt aagtataaac cgttttcata aaaaaagcaa ctatttatca
    86821 taaaaaatta aaaaattttt atttttatat gttgatatgt tgagtttttg aggagtaatc
    86881 ttgaattctt atttaaaatt taatataaaa aagttggaag aagcttccaa cttttaaaat
    86941 aggtttctat tagattagtg ggtgaagtcg agtaccatac gtccttggat ttggcctttt
    87001 tccatttcgt cgaaaatggc aacagcatct tctactggac gtttttgaac aactgggact
    87061 accagacctt ctgcaccaaa ttggaaggct tcttctaagt ctttacgagt tccgacaaga
    87121 gaaccgatga cttggattcc atcgaggact gttttaacga tgctgagttc catcgtttca
    87181 gaaggaagac caacagcgac gacgcgacca ccagcacgaa cggagtcaac agcctggttg
    87241 aaggcaactt tagacacagc agttacgaca gctgaatgag ctcctccatc agttttttct
    87301 ttgatgagtc cagctacatc ttcaacttcg aggccgttaa tcacaatgtc agcgcctact
    87361 tcttttgcaa gggcaagttt gtcattgttg atatcgactg cgataacatg agcattgaat
    87421 acttttttag cgtattgaac agcgaggtta ccaagtccac cagcaccgta aagaacaacc
    87481 cattggcctg gttcaacttt tgcttctttg atagctttat aggttgttac tccagcacat
    87541 gtgatagaag aagcttgggc tggatcaagt ccgtcaggaa ccttgacagc atagtcagca
    87601 gttacgatac attgttcagc cataccaccg tctactgagt agccagcatt tttcactgta
    87661 cggcaaaggg tttcgcgacc agttgtacag tattcgcaag tgccacatcc ttcaaagaac
    87721 caagcaacgc tgacgcggtc accgacttta aggcttttca catctggagc aatctcttta
    87781 acgataccga taccttcgtg cccaagaaca cgtcctggga cttgaccaaa gtcaccatga
    87841 gcaacgtgga ggtcggtgtg gcaaacgcca cagtattcaa cttctacaag tgcttcccca
    87901 gtttcaagtg gacggagtac tttttcttca atagcaacac cagtgctttc tggatttaca
    87961 acaacagctt tcatagctat cctccttgat attgaccgag aacagggaca acgggactgg
    88021 attgattttt tgtcaacaga gtcgagtgtg acgagctctc ttgtatttat atgtgaagta
    88081 tatcacaaaa gaaagccttt tccaaggttt tggagacaaa aagtagaaca atcgattaac
    88141 tggttgatga gactgtgaaa agagcacaaa gaccagcttg caagctggtc atactcttcg
    88201 aaaatctctt caaaccacgt cagcttcatc tacaacctca aagcagtgct ttgagcaacc
    88261 tacggctagc ttcctagttt gctctttgat tctcattgag tatcagtata ggttataaga
    88321 ataagtcttg tagggttttg gcaaccccat cttggtcgtt ggtaagggaa atttgctcat
    88381 ctgcataagg gagtagctct ggattggcat ttttcatggc ataacccttc ccagcaaaag
    88441 cgagcatttc ggtatcattg tgttcatctc caaaggcaat caaatctttt ttgtcacgat
    88501 tcattatctt gagcaagtag tccaaagcaa aggccttgtt gacacctttt ggggtacatt
    88561 caaggatatt gagcggacct ccccaggtat tgatagaaag ttgatgctgg tagaaggcgt
    88621 tcatgtcttt tgccaaggca tatttgtcac tggctctggt ctgcaagagg atacagttag
    88681 ggtccttggt caccaattca ggctggaatt gatcttcagg ctggaaagct tctacaccaa
    88741 atagtttggg attggcaatt tcttcattgg gatttgtaat gtagaatttt ttacgatatt
    88801 ctccagcgat aaaatcggct tgaatgtcct ctgaacgttg aaccatatct agcagatatt
    88861 ttttgtctac agtcaaacac ttttcaaaat cccaaacttg gtctggtaaa tgagtaaggg
    88921 atccgttgaa gttaatcata ggagtgtcta agcccagttc acggtaaaaa tcttttgaca
    88981 tacggtaagg gcgacctgtc gtaataataa cctgatggcc tttttcagca acttttttga
    89041 tggttctttt ggtaaagtca gaaatttgac tgtctgagtt gagcagggtt ccatccaggt
    89101 caactgcaat aatttttttc gtcatatgga cctttctagt gtgttttcag ataagatgtc
    89161 ttctattata acagaaagca ggcagaaaag cagattccaa acaaaaaatg aaattggatt
    89221 aatttcttaa acaaatcaaa caaagacatt gaaaaagtga ataataaatg atataattct
    89281 attattgttc gtaaaaatta aaaggagatt gatgatggac aaattattta aactaaaaga
    89341 gaacggtaca gacgttcgta cagaggttct cgctggttta acaactttct ttgcaatgag
    89401 ctatattctc tttgtaaacc cacaaatact ttcacaaaca ggaatgcctg ctcagggcgt
    89461 cttcctagcg acgattattg gtgcagtagc gggtaccttg atgatggctt tttatgctaa
    89521 cttaccttat gcccaagcgc caggtatggg actcaatgcc ttctttacct ttacagttgt
    89581 attcgggctt ggttattctt ggcaagaagc cctagctatg gtcttcatct gtgggattat
    89641 ttcattggtt attaccttga caaatgttcg taaaatgatc attgaatcga ttcccaatgc
    89701 tcttcgctca gctatttcag ctggtatcgg tgtcttcctt gcctatgtag ggattaagaa
    89761 tgctggactt ttgaaattca cgattgatcc aggcaactat actgttgtag gagaaggggc
    89821 tgacaaagct caagcaacga ttgcagcaaa ctcttcagca gttccaggat tggtcagcct
    89881 taataatcca gctgttttag tggctcttgc aggacttgcc attactatct tctttgtcat
    89941 caaagggatt aaagggggaa ttattctctc tatcttgaca acaactgttc ttgctattgc
    90001 agttggtttg gttgatttgt ctagtatcga ttttgctaat aaccatgttg gtgcagcttt
    90061 tgaagatttg aagacaatct ttggtgcagc tcttggttca gaaggattgg gagctttggt
    90121 ttcagataca gctcgcttgc ctgaaactct gatggccatt cttgccttct cattgacaga
    90181 tatttttgac acaattggta ccttgatcgg tacaggtgaa aaagttggta tcgtagcgac
    90241 aaatggtgaa aatcaccaat cagccaaatt ggataaggct ctttactctg atttgattgg
    90301 aacgacagtc ggtgccattg caggtacttc aaacgtaacg acttatgttg agtctgctgc
    90361 tggtatcggt gcaggtggac gtactggttt gacagccttg gttgtagcta tctgttttgc
    90421 gatttcaagc ttctttagcc cacttctagc gatcgtacca acagcggcta cagctccaat
    90481 cttgattatc gttgggatta tgatgcttgg tagcttgaaa aatatccatt gggatgatat
    90541 gtctgaagca gttcctgcct tcttcacatc tatctttatg ggattcagct actctatcac
    90601 tcaagggatt gcagttggtt tcttgactta cactttgact aagcttgtta aaggtcaagt
    90661 taaagatgtt catgtcatga tttggatttt ggatgccttg tttatcctta actacatcag
    90721 catggcctta taatagaatg acccaggggg atttcccccc ttttttaata caaaggagat
    90781 aggtgatgaa agagaaaaat atgtggaaag aattgttgaa tcgtgcaggc tggattttgg
    90841 tctttttact tgccgtcctt ttatatcagg ttcccctagt ggttacctct attttgactt
    90901 taaaagaagt agccctgcta cagtcagggc tgatagttgc tggcctttca attgtggttc
    90961 tggctctatt tattatggga gctcgtaaaa ccaagttagc tagttttaat ttttcttttt
    91021 ttagagctaa agatttggca cgtttgggct tgagttatct agttattgtc gggtcaaata
    91081 tacttggttc cattttattg caactgtcaa atgagacgac aacagctaac cagtctcaga
    91141 ttaatgatat ggttcaaaat agttcgttga tttccagttt cttcttgcta gccttgcttg
    91201 ctccgatttg tgaggaaatc ttgtgtcgtg ggattgttcc taaaaagatt ttccgaggca
    91261 aggagaactt gggatttgta gtcggtacga ttgtgtttgc tttattgcat caaccaagta
    91321 atttaccttc tttattgatt tatggaggta tgtcgacagt tctatcttgg acagcctaca
    91381 agacccaacg tttggaaatg tcgatcttgc ttcacatgat tgttaatggg attgctttct
    91441 gtttgttggc tcttgtggtg attatgagtc ggacattagg aatttctgtt taaaagtttt
    91501 tatgtaggaa ccgacctctt tctaccaggg aaagatgaat gcaatcgtgt ccatcttttt
    91561 ctttttatgg taaaatagaa aaataatatg atgaaaatcc ttgagggagt gaccgatatg
    91621 tcaagtaaag ccaatcatgc aaagacagtt atttgcggaa ttatcaatgt aaccccagac
    91681 tccttttcgg acggtggtca attttttgct cttgagcagg cgctccagca ggctcgtaaa
    91741 ttgatagcag aaggagccag tatgctagat atcggcggag aatcgactcg gccgggaagt
    91801 agctatgttg agatagaaga ggaaatccag cgtgttgttc cagtgatcaa agcgattcgc
    91861 aaggaaagtg atgtcctcat ctctattgat acttggaaga gtcaagtagc agaggctgct
    91921 ttggctgctg gtgccgatct agtcaatgat atcactggtc ttatgggtga tgagaaaatg
    91981 gcttatgtgg tagctgaagc gagagcgaaa gtggtcatca tgtttaaccc agttatggct
    92041 cgacctcagc atcctagttc gcttatcttc cctcattttg gttttggtca aacctttaca
    92101 gaaaaagagt tagctgactt tgaaacattg ccaatcgaag acttgatggt ggctttcttt
    92161 gaacgagcac tagcgagagc ggcagaagct ggtattgcac cagaaaatat cctgttggat
    92221 ccaggaattg gctttggtct gaccaagaaa gaaaatctgc ttcttttacg ggacctggat
    92281 aaactacatc agaagggcta tccaatcttt ctcggagtgt cgcgcaagcg atttgtcatc
    92341 aatatcctag aggagaatgg ttttgaagtc aatcctgaga cagagcttgg tttccgaaat
    92401 cgggacacgg cttcggctca tgtaactagt atcgctgcga gacagggtgt agaagtggtg
    92461 cgcgtgcatg acgtagctag tcacaggatg gcagttgaaa ttgcctctgc cattcgtctg
    92521 gctgatgaag cggaaaattt agatttaaaa caatataaat aagatgaaag aaattgaaaa
    92581 caatcagtgg attgctaact accggacgga tcaaccgcat tttggcttgg aacgaatggt
    92641 ggaactgtta gctttgcgtg gcaatcccca tctcaaactc aaggtcctcc atatcggagg
    92701 gactaacggc aagggctcga ctattgcttt tttgaaaaag atgctagaaa agctagggtt
    92761 gagagttggc gtgtttagct cgccctatct cattcattac acagaccaga ttagcatcaa
    92821 tggggaatcg atctcagaag cgaggctaga agctctcatg gcagactatc agtctttgct
    92881 ggagggagaa gcggtcgcca atttacaggg cacaaccgag tttgagatta tcacagccct
    92941 ggcctatgac tactttgcct cagagcaagt agatgtggcc atcatggaag ttggcatggg
    93001 tggacttttg gatagtacca atgtctgtca gcccattttg acaggaatta caactattgg
    93061 cttggatcat gtggctctac ttggtgacac cttggaggtc atagcagagc agaaggcagg
    93121 tattatcaaa caagggatgc ccttggtaac agggcgtatt gctccagaag ccttggctgt
    93181 gattgaccgc attgcggaag ggaaagatgc gccgagactt gcctacggga cagattatca
    93241 ggttcgtcat caagaaagtg tggtgacagg ggaagtcttt gactatacaa gtgctgtcag
    93301 acaaggtcgc ttccagacta gcctgcttgg tttgtaccaa atagagaatg ctgggatggc
    93361 catagcttta cttgatactt tttgtcaaga agatggtcga gagctagcaa gcaatgattt
    93421 tcttggtcaa gccttggaag aaacaagttg gccagggcgt ttggaaatcg tgtcaagaga
    93481 tcccttgatg attttggatg gagcccacaa tccccatgct atcaaggcct tgttggtaac
    93541 cttgcaagaa cgttttgcgg attatcataa ggaaatcctc ttcacttgta tcaaaaccaa
    93601 agccttggag gatatgttgg acttgctggg agccatgcca gataccgagc ttactctaac
    93661 acattttgcg gatagtcggg cgacggatga aagcgtgctg aaagaggcag ctaagtctag
    93721 aaatctcagc taccaagatt ggcatgattt tctagagcag aatttgacag ataaaaaaga
    93781 agagaaacaa acagttagga ttgtcacagg ttccttgtat ttcttgagcc aagtgagggc
    93841 ctatctgatg gagaggaaga acgagaatgg atacacaaaa gattgaagca gctgtaaaaa
    93901 tgattatcga ggctgtagga gaggacgcta atcgcgaggg cttgcaggaa acacctgctc
    93961 gtgtagcccg tatgtatcaa gagatttttt caggtcttgg tcaaacagca gaggaacatt
    94021 tgtcaaaatc ctttgaaatt attgacgata atatggtggt agaaaaggat atctttttcc
    94081 ataccatgtg tgaacaccac ttcttgccat tttatggtag agcgcacatt gcctacattc
    94141 cagatggtcg tgtggcaggc ttgtctaagc tagcccgtac ggttgaagtt tattcgaaaa
    94201 aaccacaaat tcaagaacgt ttgaatatcg aagtggccga tgccttgatg gactatctag
    94261 gtgctaaagg agcctttgtt gtcattgagg cggaacatat gtgtatgagt atgcgtggtg
    94321 ttagaaaacc aggcactgca accttgacga cagtagctcg tggtctattt gaaacagata
    94381 aggacctccg cgaccaagct tatcgtttaa tggggctata aaaagaatcc gtttcaagcg
    94441 gatttttcta gaaaggaatc attatggatc aactgcagat taaggatttg gaaatgtttg
    94501 cctatcatgg tctttttcct agtgagaaag aattggggca gaaatttgtc gtttcagcca
    94561 tcctatccta tgatatgacc aaggcagcta cagacttgga tttaacagcc tctgtccatt
    94621 acggagaatt gtgtcagcag tggacgactt ggtttcagga aacgagtgaa gatttgattg
    94681 aaacggtagc ctataaactg gtggaacgta cctttgagtc ttatcctctt gtccaagaaa
    94741 tgaagttgga actgaaaaaa ccttgggcac cggtgcattt gtcactagat acttgctcgg
    94801 taaccattca tcgccgcaag caacgagcct ttatcgccct aggaagcaat atgggagata
    94861 agcaagcaaa cttgaagcaa gccattgaca aactgcgagc tcgtggcatc catattctca
    94921 aagagtccag tgtcttagcg acggagcctt ggggtggagt ggagcaggat agctttgcca
    94981 atcaagtggt tgaggtggaa acctggctac cagcacaaga cttgttagaa accttgttag
    95041 ccattgagtc agagctggga cgggtgagag aagtgcattg gggacctcgt ttgattgatt
    95101 tggacttgct ctttgtggag gaccagatcc tttatacaga cgacctcata ttgcctcatc
    95161 cttacatagc ggaacgcctt tttgtccttg agtccttaca ggaaattgcg cctcatttta
    95221 tccatccgat attaaaacaa ccgatccgca acttgtatga tgctttgaaa aaatagaaaa
    95281 actctagttt tcagtcactt gcaactgaag gctagagttt ttatactctt cgaaaatctc
    95341 ttcaaaccac gtcagcgtcg ccttaccgta ctcaagtaca gcttgcggct agcttcctag
    95401 tttgctcttt gattttcatt gagtattaaa ataggtcatt ttcttctggg aggaggatag
    95461 tttctctacc gtccatgtct aaaaccagta ctcttggggg ataacgaggg tcgaaaggat
    95521 ggttaaagtc aaaatcaatg gctgtaggga ggtgttgact tgaaaagtgg aaggtaatct
    95581 ttccttggtt attaagcaat tgaaactcga gttcttcttc caattcaaag acatttttta
    95641 agaaatggtc gataatatac caaaaagagt caatgatgtc atcaggcaag ctggtaacaa
    95701 tgccaaaact agccgaccgc atgtgagtat tggtaaaagc catatctctg tcccctttct
    95761 tttcccttat catacagcaa ataggattaa aaatcaagaa aaggtgattt tttgaaaagg
    95821 attttaacta cagggaaaaa taaggaaaaa aattccaaaa aatctaccga agttaatagg
    95881 taaattccca aattaacttg attatataac tttcagttac tttgagaagt taccgaaaaa
    95941 tatttttcat atctattgac ttttaggggt gaaatttggt atgatagtag gcggtattgt
    96001 ttaccccatt tgaaaggccc cggaaccttc caaatacttt tcgatgggaa ggaacaccca
    96061 tcaccgtaaa caaaaatcga actatatata ggagaaatca tgaacaaaac aacatttatg
    96121 gctaaaccag gccaagttga acgtaaatgg tacgtagttg acgcaactga tgtaccactt
    96181 ggacgtcttt ctgcagtagt tgctagcgta cttcgcggaa aaaacaaacc aacatttaca
    96241 ccacacactg atacaggtga cttcgtgatt gttatcaatg ctgaaaaagt taaattgact
    96301 ggtaaaaaag caactgataa aatctactac actcactcaa accacccagg tggattgaaa
    96361 caaatctctg caggtgaact tcgttctaaa aatgcagtac gtttgattga gaaatcagtt
    96421 aaaggtatgc ttccacacaa tacacttgga cgcgctcaag gtatgaagtt gaaagtattt
    96481 gttggagctg agcacactca cgctgcacaa caaccagaag ttcttgacat ttcaggactt
    96541 atctaaggaa aggaacaata aagtatgtca caagcacaat atgcaggtac tggacgtcgt
    96601 aaaaacgctg ttgcacgcgt tcgccttgtt ccaggaactg gtaaaatcac tgttaacaaa
    96661 aaagatgttg aagagtacat cccacacgct gaccttcgtc ttgtcatcaa ccaaccattc
    96721 gcagttactt caactgtagg ttcatacgac gttttcgtta acgttgtagg tggtggatac
    96781 gctggtcaat caggagctat ccgtcatggt atcgctcgtg cccttcttca agtagaccca
    96841 gacttccgcg attcattgaa acgcgcagga cttcttacac gtgactcacg taaagttgaa
    96901 cgtaagaaac caggtcttaa gaaagctcgt aaagcatcac aatttagtaa acgttaattc
    96961 gaaagaatta ctatacttat acagagcacc tttcggggtg ttcttttttt atactttctt
    97021 actaaattgg tgcaattgac acagttgttg cgactttagt cgcttacaaa tgtggctaca
    97081 acctgacaag gtcagttgcc tcaaaacgtt aatcaatacg attatatcaa cgtgtcaaag
    97141 cactcaaaag tttatcttat gggtgctttt ttcgtgtttt tttgaaaagt ttaccttaaa
    97201 gtttacctta ttatgtgact ttcagtccgt ctcgctccgt taggtgcgag acaaaaaaac
    97261 cacccgctat gcgggtgcgc gtcgaaggtt ataccaaaaa actccaaacg cgatacaata
    97321 aaggtgttca agccaattgt aaagcgaaag gagaaaaata tggcacaaaa ggcacatagt
    97381 ttatcacaca caaagtggat gtgttctatc acattgtgct cactcctaag tatagatgaa
    97441 aagtcattta taatcactat cgaagcagtt tgggagaaat attccgccac ttatgtagtt
    97501 ataaaggtgt tgaaattatc gagggtcact taatgcctga tcatgtacat atgttagtaa
    97561 gtattccgcc gaggataagt gtttcaagtt ttatggggta tttaaaaggt aaaagtgcac
    97621 tcatgatatt tgacaaacat gccaatctca aatacaagtt tgggaatcgg catttttggg
    97681 cggaaggtta ttatgtgagt acggttggac tcaatgaagc cacaattaag aaatatattc
    97741 aagattaaag aaattatcca gtggatgatt tcttcacgag tataaaaatt tgagaacgag
    97801 taaagtatga tatagcacta gataaattga gtgtaaaaga atatgaggat ccctttaggg
    97861 atagtggtaa gtaatactaa agcctcttta agaggcaagt gacgagtcaa gagcaatgag
    97921 gcttgaacaa agtgaaagcc agcgttttta ggcgctggct ggtaatttgg gcttatagcc
    97981 ctgtgacaaa ccacccgttt gacgggtggt tatgattgtc gtagatgata atggttaaaa
    98041 ctaacggatg taataaatta aattatctaa tttttattga atagaattat tattccagtt
    98101 gctaaaatca tgattgataa cttcttccag ataaaaaata tcgttagagt atgtgaattt
    98161 gagaatacaa caatttgtca ttctattgtt aatagttgtt ttttcgtaat tttcccagac
    98221 tctagcaaaa tttcgaatag ctgctccatg ggacacaata aggatagagt tgcccttagc
    98281 ttccttcata agcgaacaaa tagtgttatt tattcgatct tgtaattcta attgagattc
    98341 tccaccataa tagacgaaaa agttttcgta aggaatagat ggattaagtt cttcactttc
    98401 tccttcaaaa acaccaaaat tccattcttt tagaccttta attcgtttat aaggcatttt
    98461 tccatcggtt acaatttcta aagtgtcgca cgcgcgctca gatgttgaac taaaagcact
    98521 gtcaaagata attccatgtt ctttgaaata ttcacttgct acttgagctt ggtatatccc
    98581 aaagtctgtt agcggagaat cacaccaacc ttgtataaga tgacgtttgt tgaaaagagt
    98641 ttgtccatga cgcattaaat agagagtttt tttcatttta ctatcctaca gtagattaag
    98701 ttttttaaag gcattataaa gtccatcttg tcgaacatca tctgttatca tatcagccat
    98761 agctagtatt tcaggactgc cattacccat agccacacca atatggcagg tttcgagcat
    98821 tggaatatca actttagcat caccgaaagc aattgtatca gtatgattcg ctcctaaatg
    98881 cttaagaatg atgttaacag catgtgtttt attaatatcc tttatcccta agtcaccaaa
    98941 tagagctgat tctcctctac ctccccaagt accagctttt agttgcggga aaactttttt
    99001 agaatctaag tgatcttggt aggaattgag aataaaacta attttattaa gatcgtctcg
    99061 gtacagttct ccaccataaa ctaatccatg tagcgcatct tctggctcca tgtttaaaat
    99121 ttggtcttca ctagctcctt tacctaaggc atagatgcgt agtatctgtt ttgcagcatc
    99181 acgaaaatta cggctcgcaa ataatccgtt attattttca agatagaact ctaatcctct
    99241 tgattcaagc caatcaacaa cttgtttggt ggtctcatac ggaatcagtt gatgcatgag
    99301 aacttgattt ttgtactcta tgtaggaacc atttcctcca atgataccat cgaagccaat
    99361 atctaaaatt tcgtttggca tctcagcttt acttcgtcca gtgcaaagga atactaagtg
    99421 tccatttttt tcgggattgt tggatagcta ttttagctga atcgggtatg tgattatcgt
    99481 agtcaacaat ggtcccgtca acatcaagga agataatttt tttcatattt ctactccatt
    99541 ttttagtttg attttacagt atgataataa atgtttctac tgctattcta gccaattgta
    99601 atacttttct aaggtaaaat atctattcat ctatttcatc tctatatttt cggggtattg
    99661 ttttgtaaat ttttttgaat tggcgattaa agtttgatag gtttgagaaa cctactgatg
    99721 aggcaatttc tagaacagag agagtagagt gttgaagaag ttcagtagct ttacgtaaac
    99781 gatagtggat aagatagtcc atacaactaa ctcctaaatt ttttttaaag aaattcataa
    99841 aatgtgttgg actatagcca catagttctg ctagaagatt aattgataaa tcttcctgat
    99901 agtgttcgct tatataatca ataatttttc taattttttc ttctctacga taaccatcta
    99961 ttgattgttt acttgaataa acgtaattat attgaaagag aagatgaagc agttttagta
   100021 gctcactttt taattctagt tcaaaaaatg tcttcctata gtatcctatt tccattgaat
   100081 taattagtgt ttgtcgaatt tctgtataac caggttgatt aggtttaatc actcgtttaa
   100141 agtctagttg tccattatat agggggtgta agtaatgaat tgctgcaaaa tcttttgtag
   100201 aataaccaat taagtctaat tgaaagttga ttgcatccat gtagtgataa gtattttcaa
   100261 ttgggtgtat agaatgtaaa gcattaggac gtattaaaat aatatctcct ttttcactgt
   100321 taaaatagtc tgtatctata tggaatcttg cttgtccttc gtgtacgtat atgatttcca
   100381 tatctgtatg ccagtgaaac agtatatcag gtaggccatc ttccataata gtccgggtaa
   100441 gagaataggg aattaattca tttttataag gggtattttt atgtagttgt ccgagattaa
   100501 tcataatagg cctccttcat agtatagtat tggtttttgc tattattgtg caagatttgt
   100561 aacttttatt taattataat ataagtatag aaaaaaacaa aggagaaaaa taaatgagta
   100621 aatttcctaa agaattttta tggggtggag ctacagcagc taatcagtat gagggtgctt
   100681 ataatgtagg tgataaaggt ttatcggttc aggatgtgac acctaggggc ggagttccag
   100741 taagtgataa tgatccaaat ccattcatta cagaactgcc tacagaagat aatctcaaat
   100801 tggaaggaat tgatttttat caccgttata aagaagatgt tgctttattt gcagaaatgg
   100861 gctttaaggt atttagaaca tcgattgcat ggtctcgtat ttttccaaat ggtgacgaat
   100921 tggaaccaaa cgaagaaggt ttacagtttt atgataatct ttttgacgaa ctagctaaat
   100981 atggaataga acctttagta acattatcac attatgaaac tccacttcac ttggctagaa
   101041 aatataatgg atgggttaat cgtgatttga ttggttttta tgagcgttat gtaagaactg
   101101 ttttcaatcg ttataaaaat aaagtcaaat attggttgac ttttaacgaa atcaactccg
   101161 ttttacatgt tccttttata agtggcggta ttgcaactcc agtcgagaaa ttatctaaac
   101221 aagatcttta tcaagcggtt caccatgagt tagttgcatc cgctttggca acgaaagttg
   101281 gacatgaaat caatccagaa tttaaaattg gatgcatggt tttggctatg ccaacatatc
   101341 ctatgacacc gaagccagaa gatgtgttgg ctgcgcgtga atttgagaat cagaattatc
   101401 tattttcaga tattcatgct cgcggtaaat atcctaaata tattcaaaga ttctttaaag
   101461 aaaatgatat caatatcaaa tttgaatcag gggataagga gttattagct gaaaatactg
   101521 tggattttat ttctttctct tattatatga gttcggttca agcacatgat cctgaaaatt
   101581 atcaatcagg cattggaaat ttattgggag gaattgctaa tccttatttg gaaagttcag
   101641 aatggggttg gcaagttgac cctattggtt tacgtatagt gttgaataat ctttatgatc
   101701 gttatcagtt gccgttgttt attgttgaaa atggtttggg tgctaaagat gttttaattg
   101761 aaggtgcaga tggacccact gttgatgatg actatagaat tgattatctc aaaaaacatc
   101821 tacaacaagt aggtgaagcc attgaggatg gagtagaatt gttaggttat actacatggg
   101881 gatgtattga tttagtatca gcttcaacag cccagatgag taagcgttat ggatttattt
   101941 acgtagatag aaatgatgat ggatctggga cattaaatcg ctataagaaa aaatcatttt
   102001 attggtataa agaagtgatt gaaagtaatg gcgaaacctt atatgattga ttcataacaa
   102061 aaacaagttt agatcctcat ctaaacttgt ttttgaaaat ttgaaggatg attttggagg
   102121 gtatgggatg gcattagact tcctgtgaaa ctagaatcct agctcacagt tgataattcc
   102181 agcaatcaaa ttcattcgta atccaaagcg tttacgtcga tttcgatagg ttgttgaaaa
   102241 tattttaagc ttttttattt tggcaaaaat attctcaacc ttgattctct ctttagatag
   102301 cacatgatta taggatttat cttcaagatt tagtgtctta agtttgcttg atttcctcgg
   102361 agtttgcgct tgtgaataca tcttcatgat cccttggctt gtgacaatcg cctgagcctt
   102421 catagcatga catttctttt taccagaata attggcaagt tgattttttt aggacgattg
   102481 attttggcat gtattaaaga gtagccatga ggaggtcttc taagcttaac ttaggttttc
   102541 ctccccctta tgcgtgttta agttgataag ctgtttttaa cacagctaat atctcttcaa
   102601 aagtagtgcg ctgaacgcca acaagtcgct taaatcgtgc atcactaaat agtttacttg
   102661 attcagaatt catagtttta ttggagtatt atatttttgt ttcgaaggaa gtctagatat
   102721 gttcttctga aacgcatttt ataagaaact cagttatatt tttagagtgg ttttgagcca
   102781 attattcttt gaaaagtgcc atttcttcgc tcgaaagttt tattttgaag atgacaatat
   102841 cgagtacgat ttgaaataag atggtcccct ttatatttta attattaggg agaccctaag
   102901 gtaaaaatga cgaaaaagca gtaccttaaa aggtctgctt cgtcaatttt tccgtaagtg
   102961 tccgtacact tactgtgctt gccactaacc agtagatagc ggtaagctaa ttattaatat
   103021 ggaggtgtgt caaatcctgc cagacattcg aaactttttc taacaaaagt tctcttgtta
   103081 tctcaggctt ttgaatgtaa gatccgactg tgaccatgag ggtcataagg taaatttcca
   103141 ttagatactt gtaatcttga gactttgtct tgagattttt ttcatttttt agcagtacaa
   103201 gtaaatcgtt tttatataga ttgagcatgc tcaaaaaatg atatccattc tgtactgaga
   103261 atagtaacgt ggaaatgagt aatatactat cttgagagta aaaatcattg atcagagatt
   103321 ttgaaaaatc tagaatatta gagatgaatt tattttgtat tttttccaat gtcggatgag
   103381 cgataatata ataactgtaa gtaattacat cagttacatt atcaaaaagt cgatagaatg
   103441 ttgctcgccc tacacctgct tcattaacga tatcagtaac agtaatgcta tcgaaatctt
   103501 tagcttgtag taatcgattt aacccttggc taataagtct agccgaggct aaagaccgtt
   103561 tatccgcttt gatatgatac ataaaagcct ccttttgttc cacataactt aaagctattg
   103621 taccatgaat tttagaatgg tacaattttt ataagtcttc gatgtaaagg gtttcaaaaa
   103681 agtcagactg gccagctaga cggtttatga cctttataaa ttagagacac aaaaaaggag
   103741 ttaattatgt caaaatataa tgtgttagcc aaagacatag tgacatatgt cggtggtgca
   103801 gaaaacgtta aatctcttag acattgtgct acgcgcctgc gttttgaatt gaaggatgaa
   103861 agtcaggctg ataaggaagc tttaatgaat cttgatattt tacaagtcgt tcaagccagc
   103921 ggtcagtacc aagttgtcgt tggtccacat gtcgcttccg tctacgaggc tgtcatggca
   103981 gaattgccag taattggtgg tgcaagttcg ataaatgaag atacaactgc tgaagagaaa
   104041 aagtggtatg atgtgatttt tgataccatt tctggaagtt tcacaccgct cattccggta
   104101 ttttgtggtt ctggtttggt caaagcgctc gggtcagttc ttctcatgac aggactattg
   104161 acagcagaaa gctcaaccta cgccgtcttg aatgctgcag gcaatgctgt cttttatttc
   104221 ctacctattt tactcgcaat ttccgtcgct aataaactgg gaaccaatcc ttacattgct
   104281 ggtgccattg gagcagctct tcttgaacct agcttcacag ctctggctgg aaatgagagc
   104341 aatacttttt tgggtttgcc gattatcatt aacaactatg cgtcgtcagt ttttccagcc
   104401 tttatggcag tcgttggtct gtattttctg gataaatttt tgaagaaggt tgttccagca
   104461 acgattcacc tggttgtctt accatttatc gaaattgtgg ccatggtgcc tttgaccatc
   104521 tttattttcg gaccattcgg gacctatctc agcactggct tgtccatgtt tgtcactaat
   104581 ctactcgatt tgaatgccat gttagccgga gcagtgctca gtatgatttg gatttatgtg
   104641 gtaatgctag gacttcactg ggcactcatt ccaattatga ttaacaatat tgcgacaaat
   104701 ggttctgacc ctcttatggg gcttatgatt ggtacggttt gggtagctgg aggtgtttct
   104761 cttggtgttg cattaaaagc taaagataag aaactaaaag gtattgcctg gactggcatg
   104821 attccgtgtt tcttgtctgg tgtatcagag cctattatgt acgggattct tttccgctat
   104881 aaaaagtcat tgatgtgggc aaccgtcatg aacgctgtca ctggtgctat ggcaggattg
   104941 attggcatta gcggaacgca aattgcaggt ggtgttttca ctattccaac atttagtcca
   105001 atcctcggat atttggccct aattgcggta tctgtttttg gaacagcagc tctcattgtc
   105061 atttttggat ttggtgaaga aggctcaaaa gcttagaaag cgtgacatca tgaaagttgc
   105121 ttaccataaa attcctatac cagtccaact gccttgtcat atggaaggtt atgtagatcg
   105181 tgttagctct aaaattcatg accatctaga acttgtttcc ttcattttac aaggtgagac
   105241 aactattgtt tatcatgttt tagatgttat tttgatagaa aaagaactgg ctaatcgctt
   105301 acaaaatgca atcaatcaaa aatttcagat accgatagaa caggttttca ttgctgccac
   105361 acatactcat tccagtgtta aaatttcgaa ctatctctta cccagttatc caccagaagc
   105421 tgagttgtta gataagattg aagaattagt tttggaaaat acggctatct gtctagcaaa
   105481 tctaaaagaa agccaagcca actatcgtga gatttcaaca ggtcatcttt atagcaatcg
   105541 taatgatcct agtcacccct tcaatgataa ggcctatgcc attcgttttg aagacaccaa
   105601 tcagcaacct atttttgagc tggtcaacat tgcctgccat ccgacagtct tggcaccaga
   105661 ggttacagaa atttcaaggg attatgtggg tgtcttacgt gatgattatg agaaggcgac
   105721 aggcgtaccc ttggctgtct taatcgggga agcaggagat gtcagtaccc gccatacaag
   105781 aaagggccaa gacttttctg aagtagagcg cgttggaaag accatttctc aactcttaca
   105841 aaacgaaaaa aacagtcacc tattggactt gtctggtatg catttacgac aaaaaacggt
   105901 ggagtttacc tataagccgc aagaagacca agatttatta atgactcgaa aactttatca
   105961 agaacagttg aaaaaccttg aaggaagcaa gcaaggtgag attatctcag atttccttcg
   106021 gatgattgat aaaaaattgc aaaaagagag tttaactcta accgttccga tatcagtttt
   106081 agattgtaag gactggcaaa tactacaatt tccgagtgaa attctatctg attttgggtt
   106141 aaagattcgt gaacaaagtc agaaaaaaca ggccattcac gcctatacta atggttttat
   106201 gggttatgcc gtcccagaac atctttacgg caaggtttat gagtcggctg tcagcgaatt
   106261 tcctcgcggt atcgcggacc agctgattga acaactgatt actttaagta aatagataga
   106321 ggtgctttat gtcttacatt caacttgatt tccattctca ttctcttaat cgtaatgtta
   106381 atttgaacat tttcttacca gatggtgaac tcttacctgc gccagagcaa ccttatccaa
   106441 ctatttattt cttaccaggt ttttctgcca gtggtaagga actgtcgacc ttcctacatt
   106501 tccgtacaca atgtcttctc aatggtatcg cagttgtgat tccagatggt gacaactcat
   106561 tttatctaga taaaccagaa cgcttagccc tttatagtca gtatgtgtct caagagctta
   106621 ttgactatac ccgttcgatc ctgcctttgt ctcataagag agaggaaacc tacattggtg
   106681 ggatttctat gggaggttat ggtgccttgg ttcatggctt tagatatcct gagaaatttg
   106741 ggaagattgt ggccctttca ccagctattg atgtagcagc tattttaaat ggcaatgcgt
   106801 cacaatttgg tacgactttc gcagacttat tcaagacaga agaagactat tatagttcaa
   106861 ccgatcatcc agttaatgcc cttaaagaac ttttggataa gggagaggat attccagaac
   106921 tctttatcgc ctgtggattt caggatgagt tggtttatgc ctctaacagg tcatttgcgg
   106981 aagagttaga aaaattaggt gttaacctga cctatatcga agatgatgga ggtcatgatg
   107041 ttttcttctg ggacaagtac ctccctgctg ttttcgagtt tttgagaaaa taatctcgtg
   107101 tgttatccta gtgtaggtag gcaatctgtc gcctacactt ttcctatttt gattgaaaag
   107161 gagtatctat gaaggttcag acctatacac ttaatgatac ggctcatgtg acagcctata
   107221 ttctggatag tcagatttct tatggtgttc atcgtaagcg ccctgctatg attatttgcc
   107281 caggtggtgg gtatctgacc ttggcaacta aggagggaga agcagtggcg actagttttc
   107341 tcagtcaggg ttatcactgt tttgttcttc gttatagtac ctatcatctg gagcgggtga
   107401 caagtttaga ggagacgccg attatcaatc ccaaagcccg ttaccctcag ccgattttag
   107461 aactaatgca gactatgcta ctcttacatg agcatgcgca agaatggcat ttggatgatg
   107521 acaatatttt tgtcacaggc ttttcagctg gtggtcatct agctgcaagt ttgggcgttc
   107581 attgggacaa tcctgacttt catcagattt tagagcaaga ctttgagcca gaagatttaa
   107641 agcctaaagg tttagttttg gcctacccac ttttggaggg tcatggtggt gactatatcg
   107701 caaaaaacac gcccaaggag catttaattg ctcatcagct tgctgacatg tccgattttt
   107761 tcttccaaac gcaggaaccg agtcaagttc aaaaggaagc agttagtatt ttacccttta
   107821 tcaatgaaaa gaccccacca actttcctct ggataacagg gcaagatcag atagtggatc
   107881 ccagagcttg tctggtctat gccattaaaa tgcaggaatt ggggcttgat tgtgaatgtc
   107941 atctatttaa taatggtcct catggactgg ctagggctga taaggtctat gctaagtcat
   108001 cgacagaagt caatccacct gttggcgttt ggatagagat ggccctgact tggctagatg
   108061 gacaaatgga gaaaaatcat gaatattaag acttatcgaa catccaaaga gaggcttttt
   108121 gcggactata tcaaggcaga aaaagaaaga gcggataaga aaatctatag tctggatttg
   108181 cctgagattg gtcgcctgac agcctatcta tatcagccag aggataaatc tcggatggcc
   108241 gaccagccta tattggctgt ctttcatggt ggaggttttg tcctaggcta tccagagcaa
   108301 gaaggtcctt atgctaggaa aatggcagag cgactaggtt gcttagttgt ggtattggat
   108361 tataagttgg ccccagagtt tccatttcct ctacccataa caagtagtta tgattttctc
   108421 agccaattta tgcaagaaaa gtccaacctt ggagtgtcat cagaggaatt gattttactg
   108481 ggacatagcg cgggtggcaa tctcgtctta gcaatagaac aactcaatca agagcgtcaa
   108541 ggacttcaca ttaagcaagt gattgttaac tatcctgtac tagactggcg acagtggtcg
   108601 caagacaagt cacgcttagg agattattgt cactggtatt tagggaagaa tttttcaaat
   108661 atccattacc cactggcctc tccggtatta gcagaaggtc taaataaacc aaatattttt
   108721 ctcatggttg ctagtcttga tcctttagca ccccaagctc acgatttttt ccaaaaaaat
   108781 cctaaaatta gctataaaga atatgaaggt gcccaacatg gatttacaca tttttggtat
   108841 caggaatttg atgccgaaaa atcagcagag gcttgggaag atattataag atttattaag
   108901 gaaggacaac tatgaaagat attttgatga gaatagatga tttgggatat tctgaaggag
   108961 ttaactatgg tattgcacga gcagttaagg ctgggcctgt gaaccatgtc ggcctcatga
   109021 ctaatatgcc ttgggcagcg catggctatg acctaatcaa agatagtggt gtcagtctag
   109081 gccttcacgc taatatcagt gcgggtcgtc ctctcagtcc cttggaagag gttggtagtc
   109141 tgatagtaga gggacatttt aaatcttcct ctacctacaa tcaagctagg tatcaggata
   109201 tttcagtcca agatgtgatt acagaaatta ggcaacaagt agaagctttt aagatgctag
   109261 taggtaaatt accagactac atcgatgtgc atgcggtttt tcatgatact tttaaccaag
   109321 gagtagcaat ggttgcaaag gaatacggtc taccatttgt ggatgtcgta gcaggtaaga
   109381 ttcatgggac agataagacc attcactatc tgggtggtac cagtcaagag gcagatttgc
   109441 ttttaccgac cttgcaaaaa caagcagaag aaactgagca tttacccctt tttgtttatc
   109501 atccaggctt tattgatcag gatttgatag ctacctcaag cctaacttta caaagagtcg
   109561 cagacgttgc ctttttgaca gggcaagaat taagagacta tttagagcaa gcagagttgc
   109621 atttgtttga tatacgagac ttggaaaagt tatgaaacaa attcctttct aaatgtttca
   109681 cttagctact atctattgtt ttggctaata aggattgcta aaatgagata aatagaactt
   109741 agaaggtagt aaaataatgt taaaaaaata tctttcctta tttttaggtt tgattatgac
   109801 cattttagtc tgggctcagt cgattttaat aaattttgaa gtgcctttgg ctatcttgac
   109861 ttttatctta attatcggtt ttgtattttg gaataataga aaaactgatg agcaaaataa
   109921 aactctcata tggcttggct tatcttatat tttggttaga acaatttggt ttttctttag
   109981 tcatgttagc ctttgggggt tagggcaaat actactaata attcttagta taggattgtt
   110041 aatcaagggg ctgcattttt caaataaaaa tattcctatc caaattttaa aaggtttatt
   110101 tttgactttt ctgactgtaa ttactctatt actaacgtat ttgaccgttg attcaagtcc
   110161 tatcgctaaa ttaactcaga gcgtggcaaa tacaattaac agttttgaac cacagacaga
   110221 ttttacagtg actgtcagtg aggggaatca gctattagaa aatatccaat atgggacaga
   110281 gttaccaaat agttatttgg atattatctt gccagaaggt gacaatatta actctcgacc
   110341 aacttatata tttttccatg gtggtggttt tgttgcaggt gataaaatgc aaggagatcc
   110401 aaatcaagtt tctgaataca gtgccagtct ctatcatttt gaagaaatgg tcaaagcagg
   110461 atacaatgtt gtcactgtga actatgcact tgctagccag tatccctacc ctacggccat
   110521 taaacaactt tctgatgcgg tagtatttct tgaaaaaaat gctgaaaaat atcagttaaa
   110581 tatgaatgaa gttgttattg ccggtgctag tgcgggtggt aatttagcag cagaatttat
   110641 aacaatccaa gctaatccat cttatgctga agagcttaat gtaacaccag ttttggactc
   110701 gtctaaaata aaagctgccg ttctagaagt acctgctctt gaccctaatc gagtcgcgaa
   110761 aactcatcgc ccatcaataa tagaggacta cgtgtttggc catgctcttg cagcttatat
   110821 ggaacaatca cttgttagcc caaatagaga actctatgcg tcacgcaatt tgattaacaa
   110881 aataacagct gatttcccgc ctgtttttat tacagatggt aatattggtt cctttatgga
   110941 tcaggcacaa gattatgcta agaaattagc agaaaagggt gttacttatg aactatatat
   111001 tccagatgaa gcaacttatg gggatcagcc acatggttac ttggcagata tatattcaga
   111061 agctacgcaa acttatatta agaaaaaaat ctcattccta gaaaaattaa ctaactagct
   111121 gttaaaatag aaaaaaatta tccatgaaaa tattctcatt attttatatt ttcattgttt
   111181 tttgttaatc gaaattttca tagtccaatt tggactatat ccacgataat tatttttaat
   111241 atcctataat cctcgtagga ggagttgatg taccgacgtt taagtgattt gagagatgat
   111301 ttggatattt tccagtctga aattgcagac tttcttcatt gtacgcagtc tacttattca
   111361 aaaattgaat gaggtgatcg agaaattcct atatctttct taataaagtt agcaaatttt
   111421 tataatagac ttcctgcgaa actagaatcc tagttcacag ttgataattc cagcaatcaa
   111481 attaattcgt aatccaaagc gtttacgtcg atttcgatag gttgttgaaa atattttgaa
   111541 ctgttgcttt tttatattat gtgactatta gtccgtctcg ctccgttagg tgcgagacaa
   111601 ataaaccacc cgctatgcgg gtgcgtgtcg aaggttatac caaaaaaatc cgaacgcgat
   111661 acaataaagg tgttcaagcc aattgtaaag cgaaaggaga aaaatatggt acaaaaggct
   111721 catagtttat cgtacacaaa gtggcactgt tctatcacat tgtgttcacc cctaagtata
   111781 gacgaacagt catctataat caatatcgaa gcagtttagg cgaaatattc cattgcttgt
   111841 gtagttataa aggagttgaa attattggag atcacttaat gccagactat gtacacatgt
   111901 tagtaagtat tccaccaagg ataagtgttt cgagtttcat gggttattta aaagtaaaaa
   111961 gtgcactcat gatgtttgat aaacactgaa agttctaaaa tgaagttagc cacttccgag
   112021 taccaaaaag atctcagaga ggtaaaattg taatcaccac aaaaacaatt agaaagaccc
   112081 tgagatgcaa gactgttata caccaagatg atttttctat gactgttctt gccttttgcg
   112141 ttttctccca tgaacttttt cttaaaaaaa tcccttgcca ggaattggac aagggatagg
   112201 attcttttag tctttaacag catcgcttac actctttgcg acgacttttg cgacaccttc
   112261 ttggaatggg tctggaatga tattggtagg acttaattct gaatcaggaa tgaggctggc
   112321 tatacctttg gcagcagcaa tctgcatctc tactgtaatc ttcttcgcac gtgcatctaa
   112381 agcaccacgg aagatacctg ggaaggctaa tacattattg atttgattag gaaaatcgct
   112441 tcgtcctgtc ccgacaatat aggcacctgc ggccagagcc tcatcaggga aaatttcagg
   112501 agttggattt gccatagcaa aaataatagg cttgtcattc atcttggcaa tccactcagg
   112561 attgagggcg cctggggcag aaacgccgag aaagacatca gcgtcctcta aagcatctgc
   112621 caagctacca ctagctaagc gtcgattggt caccttggca atatcttggt gatgtggagc
   112681 cagcttgtca tggtcttctt cgctgatgat accaaagcga tcgacaactg tcacatctgt
   112741 agcacctgca gctaagagtt tgcgagtgat agataatcct gctgaaccgc caccattcac
   112801 aacaattttt actttatcca actctttttg agctagcttc agactgttga agaggccagc
   112861 caaaacaacg attgctgttc catgctgatc atcatggaaa attggaatgt cgcattcttc
   112921 aatgagtcgc tgctcaattt caaaacaacg tggtgcagaa atatcttcca agtttacccc
   112981 accaaaggta ggcgccattg ctttgactat acgaatgatc tcttctggat cttttgtatc
   113041 caaaacaata ggcactgcat caacatccgc aaatcgttta aagagcgcag cttttccctc
   113101 cataacaggc atggctgcag ctggaccaat atcacccaag cccaatacag ctgtcccgtc
   113161 gctgaccaca gccactgtgt ttttctttgt agtcagtgta taagctagat ctgcattttc
   113221 tgcgattgca gaagatacgg aagcaacacc gggtgaatag gcaatactca gatcatggcg
   113281 attattaacc gtaacttttg agactacttc tagttttccg ccattcgctt ttgcttgttc
   113341 aatggccaat tgacctacat tttctaccat tttgataact cctttttatt attttattta
   113401 ttattttatt cttatttaaa aatcattcgt aagatagcgg tcatgataat gactgtaatt
   113461 gccccaccta gtcgagtagc aacttgggca aatggcatca agttcatccg atttgctgta
   113521 ctcaggatgg caacatctcc tgtccctccc atgccacttt gacaagatga aataatagca
   113581 gcttccacag gatgcatgtt caagaaacgg gagacaaaga aaccgactga aacgacagta
   113641 aagacaacac tgatgaccac aataaagtac ggtaagctta aggtggctac tacatccttc
   113701 aaaggaatat agagcaagcc aagtcctgcc ataagcggga aggtaaagtt tcctgaaata
   113761 aatttataga gttgctttgc accgttttgt gtttcttgtg ggatgacatt gacatacttc
   113821 atcaaggctg caatgacaat cataaggact ggtcctggga atccagtcag gtgctgcatg
   113881 aggtggcctg agataaaaag ggtacatgcg gttaagaccc ctgctcccat cattttgaca
   113941 tcaagcggac cagacttatc ttctaaaata tctgataaat cttccttgtc gctaacctta
   114001 accaactgcc cctgtcctga cagttcaggc tttttctcac caagtcggct gaggagagct
   114061 gtacaaagaa tggcaaagaa gtttccgatg atagtagccg gaatcagctg agcaaccaat
   114121 tgttcactcg atacacccgt aatagcactg tagccaatgg aaagcggaag gattccttca
   114181 ccaattcctc cagcaaggac tggggttaca atgtagaaga gggtatgttg ccaatccagc
   114241 cccagaagga caccaacaag ggttccaact gccatagcac aaatcattcc tagaagcatc
   114301 ggaataatca tacgaaaaag accctgaatg agcattttac ggttcatccc aaggatacta
   114361 ccagagacca aacaagcaat atagaaatac agaaaattcg cttgtttcat caatacattc
   114421 gtagcttcca aaacattggg gttaatcaaa ttgaaaaaga cgagaataga aggcaccaaa
   114481 agcgagagaa tcgctgggcc accaaagttt ttcaagccag gaatactagc tcctattgtc
   114541 ccgagcaacc aacctatggt aaggattact gcgaatccgc ctaacatatt gacaggtagc
   114601 tgctgtaaaa gccctgccgc taagataagg gctgacagac aaaggtatac cggcaaggga
   114661 acggacccga ccttaacatc tgaaatggat tgttttgttg catccaaagt tggctgctgt
   114721 tttgctttca cgctagccat gaaagtacct ccttttaagt tcttggtttt agtataaaag
   114781 aagtctgttg ttctgtgaag attatttaat taattgaagt atttcaattt atttaattaa
   114841 tttaagctag attctctaaa aaaatatgtg gtatactaaa agtggaggca ttatatgaag
   114901 aaaaatttat ccctttgggc ctttctttcc ttgtttttaa tcggggtgtt aaccctgata
   114961 ctatctatct tttactatga aacaactcat gaatcctatc agattattcg cattcaggaa
   115021 gagcgttttt taaaaacagc tggtcggatg attgctaaga atcagcagat tatcgctatg
   115081 ctaaagcagg gcgaatcaga tcaacaggtg caggactaca cccttgcatt ggccgaggat
   115141 tctcatctgg attatattgt agtgatggac ttatctggga cgcgattaac tcatccgaat
   115201 ccttcaaaaa ttggagaatc ctttcaagga ggagacgaaa aagctgtcct caatgggcag
   115261 gaaatccttt ctgtcgctga aggaagctta ggggagtctc tacgctacat tgttcctgtc
   115321 tatgatggta agcagcagat aggtgccgtt gctgttgggt taaaattgac gacattaagc
   115381 gatatttccc agcagaatat ccaacagtat acacgtgcat tggtcattag cattctcctt
   115441 agtttactgg taaccagtag catttcctat tttcttaaaa aacggctcca taatctagag
   115501 ccaaatgaga tttttcaaaa tcttgaagag cgcaatgcca tgttggagca gactcatgcc
   115561 gctgtctttg tccttgacca acaacactat atacagcgtc acaatccggc tgccgtccgt
   115621 ttattaggcg cttattctaa taataaagag ttaattgggg aacgcttgga tgatctcctt
   115681 cctatgctca atgagaagca ttttgcaacc cagaacgaac aaattgtttc attcagaaac
   115741 caggattatc tcttgtccat ttctcctata gttgtgcaaa atcaaaatcg aggctttgtt
   115801 gtctttatac gcaacgcaac agaaacccta ttgcagatgg aacaactgac ttataccact
   115861 acatacgcct ccgcattaca agctcagacc cactctttta tgaatcaatt gcatgttatc
   115921 tatggtctcg ttgacattgc atattatgat gagcttaaag tctatttaaa cgagctcttg
   115981 gagccacgga atgaattttt gaccagtcta tccatgttga tcaaagaccc ccagctggct
   116041 agttttctta ttggagaaaa agaaaagttt tctgaaaaac acacccaact gcagtttgaa
   116101 atacgaggag aagtcggcct gccacagata gcagatcagc tccatcacta catttttatt
   116161 catcggtata tcaacacctc attgctccat tttacattgc cagagaccat ccaaatacag
   116221 ttgtcttatc aggataaact gttgcagaca gaatatcgat tttctgctcc aacatttttg
   116281 agcgataatt ttctagcttc tcttcaagaa ccctattttc agcaactact tgcagatgca
   116341 gatggagagt atgagtttaa tacgcaagaa gatacgattg ttatttctac acgcattacc
   116401 tattaaggag acggaaatga acgttttaat tatcgaagat gatccaatgg tggaatttat
   116461 tcaccgtaac tacttagaaa aagtggctat ttttcgtgaa atttactcta ccaacagtca
   116521 gattgaagca catcactttc ttgaaaaata tccgattgac ctcatcctgc tagatatcca
   116581 tatcaaggat ggcaatggtt tgatcctgct cgaggagtta cgagccattc atattaatgc
   116641 agaggttatt gccataactg ctgctagcga ggctcaaata gtcaaatcta gttttcacct
   116701 tggagtgatt gactacctca tcaagccctt tacgtttgac cgttttgaag tagccatcca
   116761 aagattttta caacaccata aggcctttga tgaagctacc gtgggacaag aaactattga
   116821 ccactaccat cataaatttt ccgttgctaa ccctgttagc catcaaggat tagatgaaaa
   116881 aggtttgtca gaaaccactt accaacatat ccttcaagca atctctcgtt taaagcaacc
   116941 ttttaccatt caagaattgg ctgatgctag tcaattctcc catgtgtctg tccgaaaata
   117001 cgtcgcctac atggaagaac agggggagct gataagtgag caaatttata cgaaagtcgg
   117061 gcgccccttc aaagcctatc gtaaggctat tcaatagctt gaattaatta gacaaatata
   117121 tacatattcc gcaaaaaacc actcgatgtc agttttctta aatagcatcg agtagttttc
   117181 ttcaaaaagc tgtatgtgtc aagtaaaact cggaaaattt aaaaaatccc tatagtaaca
   117241 ttttaactga tttgtttaaa ggatttaaga ttccatatgg cgtgactgtt gcagcgtgta
   117301 gagttttgtg agtgtcctgt ctcgcttttt taacagacga aggaaagttc aggctgtatt
   117361 ttcagttttc tttcgcctca gataaattag ggttgtagaa agagcaagta gaatcaagaa
   117421 aatcaaaatt gtatggtaga catccggaat ataatgtgtt tccgaacgta tctactgcag
   117481 ggaatgagta aaataagcct tcaattcttg accactacca aggagcatag ggagcaagtg
   117541 aatccaaggt tgcaggatca accagagact aagccagagt gtcaagaagt acttgcccac
   117601 tctttattga aagataaaaa ggcaaataca gataaaatag atgagaaata aaagcccatc
   117661 ttcccgccac gaccgactaa caagataagt ctcgccaaaa tcaagtcctg tcatatccag
   117721 agaaaacaaa agcaataaga tttggaatta ggctcgtcaa gtcttgagga atatgttgat
   117781 ttacggttgt tagagataat ttgtggtgaa gcttcttaaa ttgttactcg ctatgataca
   117841 gtgactggtg agaaaattac attatctgag ctggttggtt ttgttaatag aaaattacag
   117901 tgcattaatc gtgaagttat agatgcagtc atctaaaaat ttctatttct ataaacaatc
   117961 tagcagaata ataacaattt aaatggtgtg aaatttttag taaattctag ctcaatttat
   118021 gtatttttag ggtaaaattc acaccattta actgaaactt attcaaatta gatgaaatta
   118081 gttttctaaa aaagcgggga aacgctgatt taaaggggat tttgaaattt attcaaatat
   118141 aaatgtatcc aaccaggtct taagaaagct cgtaaagcat cataattctc aaaacgttaa
   118201 tcaatacgat tatatcaacg tttcaaagca ctcaaaagtt taccttatgg gtgctttttt
   118261 cgttacgacg ggcatgtcgt gcatctgagg tgtaagtcct ccgtgggcac ctgctaccgg
   118321 tgaacccaat agcgattccc aagcctgact atcgtgaggt agcggattaa aacggtctgg
   118381 ggatagaccg ttttaagtct gacactggaa ataagaatcg tcagaggaag ggatagcgaa
   118441 atcgtggctc tacgaacagg aacgtgatag taaggcgtat atagcggata agggggcgcg
   118501 aaacactaaa gtccaaaaag gtagccgtaa cctatatgtg taaatcacga gagtaattga
   118561 attcggacta aggtttgtgt gaaaaagata aatctttcta gagtctaaag actctgcgtc
   118621 agatttccta ttttcactgt aaccttttaa cgtcctcata tcttgtataa acgaggaaag
   118681 atgtacgact tatcccgtga ggtttcatga gcgctgaaag cgtagtaaca acgaatcatg
   118741 agaagtcagc cgagcccata gtagtgagga aacttctgta atggaaatgg agcgaagggg
   118801 taaacaacta attgaagtaa tcctacttca cttgtgtctg taaaacgaac ggtctgatag
   118861 aactggactc tgtcacgtat tgactagatg aaggttcacc aatataagat gtccctcagg
   118921 caccaaaaca agaaaggaat aggcacatgt caaaattgct agataaaata ttatcacgcg
   118981 acaatatgct
//
LOCUS       DAVDWT010000002       109748 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVDWT010000002 DAVDWT010000000
VERSION     DAVDWT010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 109748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 109748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..109748
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.002"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..222
                     /locus_tag="VUW50_000126"
     CDS             <1..222
                     /locus_tag="VUW50_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HET7982102.1"
                     /translation="FQTVFKTFLKDKEKIVNALQLPYSNAKLEATNNLIKLIKRNAFG
                     FRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(294..944)
                     /locus_tag="VUW50_000127"
     CDS             complement(294..944)
                     /locus_tag="VUW50_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET7982103.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            1056..1898
                     /locus_tag="VUW50_000128"
     CDS             1056..1898
                     /locus_tag="VUW50_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET7982104.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            1974..2411
                     /locus_tag="VUW50_000129"
     CDS             1974..2411
                     /locus_tag="VUW50_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET7982105.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            2480..2783
                     /locus_tag="VUW50_000130"
                     /pseudo
     CDS             2480..2783
                     /locus_tag="VUW50_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2874..3947)
                     /locus_tag="VUW50_000131"
     CDS             complement(2874..3947)
                     /locus_tag="VUW50_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HET7982106.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSRQVDT
                     TFKEWVTTVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     EKETLGRLTQLLIDTRYQVWKNR"
     gene            4103..4780
                     /locus_tag="VUW50_000132"
     CDS             4103..4780
                     /locus_tag="VUW50_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7982107.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            4773..5747
                     /locus_tag="VUW50_000133"
     CDS             4773..5747
                     /locus_tag="VUW50_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET7982108.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(5796..7739)
                     /gene="thrS"
                     /locus_tag="VUW50_000134"
     CDS             complement(5796..7739)
                     /gene="thrS"
                     /locus_tag="VUW50_000134"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET7982109.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELVSGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(7797..8120)
                     /locus_tag="VUW50_000135"
     CDS             complement(7797..8120)
                     /locus_tag="VUW50_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HET7982110.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            complement(8551..8751)
                     /locus_tag="VUW50_000136"
     CDS             complement(8551..8751)
                     /locus_tag="VUW50_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982111.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKELYINNLL
                     FFTPLVILIISLLYSYRFRFSL"
     gene            8990..9619
                     /locus_tag="VUW50_000137"
     CDS             8990..9619
                     /locus_tag="VUW50_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7982112.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTPDFPEEFKPLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            9760..10029
                     /gene="rpsO"
                     /locus_tag="VUW50_000138"
     CDS             9760..10029
                     /gene="rpsO"
                     /locus_tag="VUW50_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET7982113.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            10845..11459
                     /locus_tag="VUW50_000139"
     CDS             10845..11459
                     /locus_tag="VUW50_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HET7982114.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(11751..12500)
                     /locus_tag="VUW50_000140"
     CDS             complement(11751..12500)
                     /locus_tag="VUW50_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET7982115.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            12633..14969
                     /locus_tag="VUW50_000141"
     CDS             12633..14969
                     /locus_tag="VUW50_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET7982116.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHNYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVIMLVFTVIFILTSRYQARK"
     gene            complement(15406..18552)
                     /locus_tag="VUW50_000142"
     CDS             complement(15406..18552)
                     /locus_tag="VUW50_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HET7982117.1"
                     /translation="MIRTEVTSMTVPDRLPARMRMDGRSIFQQIKNYFDTENKEYFKH
                     PNTYDGISMHLEPNILTSMEHFDLTGFHCECKDFQNQGVCKHWVAMDLYFRSLPQAIQ
                     ERIGKASHKPSFASQLIPTLPSEELQDELVEEKATAPSLALHGQVEIRNHSLAWTLKL
                     QVEQAPRAYVIKDIAHFIFLIFQKEDYFVSQKIGTIRLSLNQFNQASQNLLLYIKKYF
                     IDRNEHSYFNFSYGINPRDYGRYLETPVSYLNDLVPLFQALDVFQYVTSKAEYPLIFL
                     DDSPFIPEEEIFKVVKSNNHYEIINTAYFGFIIQEKLWIRHNHFHIIKEEHRYFLDKL
                     ATWIYHYQENSPLIFSKENKAELMQVCNIISNYVPISIPDELQIHDFIPTFAFSKTRN
                     EIALNMVWSFGEKQVHSKQDLLTLPYTYQASKARKIYHQLLSAGFKEEFHSLSKIKIV
                     DFFLKELPRFRTLGQVQLDESLEKLLVEDPAVIDIFDDESFLSVQFDFSMISEDEVEK
                     AIQALWNQESHYQTKQGKVLVFDDESLKVAQSLQDLRAKFSDGKIKMHKSRAFSLSET
                     FKDNEHVNFSRDFKKMAYDLTHPEEFDIKPYEVKAKLRSYQKEGVKWLSMLDHYHFGG
                     ILADDMGLGKTLQTITLLEANLKPDQKALILAPASLLYNWKEEFRKFVPHKQVEVAYG
                     SKTERIKQIEKSATITITSYPSFRSDLEHYQKQSYDYLILDEAQMIKNSQTKTAQALR
                     EFDVKTCYALSGTPIENRLEEIWSIFQIVLPGLLPSKKEFSKLSPQLVAKLIQPFVLR
                     RKKDEVLTELPELSEHLYSNELSSSQKTLYLAQLRRMQEMVSGASAYEIKRHKIEILA
                     GLTRLRQICNTPALFLEDYKGDSGKMDSLFELLDTIREKGSRPLIFSQFTSMLDLIEQ
                     ELEKKEMSHFKITGQTPSDKRQEMVNLFNQGEKDCFLISLKAGGTGLNLTGADTVILC
                     DLWWNPAVEMQAIGRSHRLGQTKQVDVYRLITLGTIEEKIQELQESKKELFNTVLEGQ
                     ESRSNLSVDDIKEILGVE"
     gene            complement(18741..19979)
                     /locus_tag="VUW50_000143"
     CDS             complement(18741..19979)
                     /locus_tag="VUW50_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982118.1"
                     /translation="MHIFLKNRAFRQLTVNEWISSFGDTIFYLAFINYVSSYAFAPLA
                     IFLISLSETIPQVLQLFTGVIADFQKNRISKYISILFIKVLLYSGVTLLLTSTDFSLF
                     SVFFICSMNLISDTIGFLAGYMLTPIYIRLINDDMTEAMGFRQSTSSIVRLIGNLSGG
                     VFLELFSISTLAFVNVLTFLFAFLGSLLIRNRLKKEEEKIEVPPYVGMSSFFQHLKES
                     MKLLMTMEDVMVLLWILSISQAVLMMVEPVSAILLIHHPFMGLSTGQSLAILIMISLL
                     HVILGGLLSGFLSKKISIRLNIYWSLLMESLIVIDFLRGSFLLILLGSAGDAFSAGVL
                     SPRLQAMIFGIIPEELMGSVQSSINVINLLIPAVLSLALVFLATSAGLEVVAFALIIL
                     LLIAAYLVHQMKNLPNQEEV"
     gene            complement(19981..21418)
                     /locus_tag="VUW50_000144"
                     /pseudo
     CDS             complement(19981..21418)
                     /locus_tag="VUW50_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
     gene            complement(21492..22022)
                     /locus_tag="VUW50_000145"
     CDS             complement(21492..22022)
                     /locus_tag="VUW50_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HET7982119.1"
                     /translation="MLDEKRKNYVEDKRQNFPSLPSWKMDLFSEEEKRIYFQTTCEVS
                     SKDSAINKYKMEKIIKRSNKGNVYRAIRKSDGQKVIIKQSRPFVNYDAEGEWTALDDI
                     KNEAHMLKKLADKSYTTNLTDEFYIVDDYFLVQEQVDGLNFEEFIRETEHSLNIREKT
                     LDNIVNIVSYIHKLGI"
     gene            complement(22016..22484)
                     /locus_tag="VUW50_000146"
                     /pseudo
     CDS             complement(22016..22484)
                     /locus_tag="VUW50_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23033..23710
                     /locus_tag="VUW50_000147"
     CDS             23033..23710
                     /locus_tag="VUW50_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HET7982120.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLCKEVSPVFVQKWQKEAVKLEFALGQIPDKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            23697..24494
                     /locus_tag="VUW50_000148"
     CDS             23697..24494
                     /locus_tag="VUW50_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET7982121.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLEIEETTYLQETGPERGIGRIGNIQPQTFGELAQHVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            24511..25614
                     /locus_tag="VUW50_000149"
     CDS             24511..25614
                     /locus_tag="VUW50_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7982122.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESNLE
                     ELYQLALQRREESPLIGQLAILNQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            25915..26934
                     /gene="galE"
                     /locus_tag="VUW50_000150"
     CDS             25915..26934
                     /gene="galE"
                     /locus_tag="VUW50_000150"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET7982123.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            26936..27871
                     /locus_tag="VUW50_000151"
     CDS             26936..27871
                     /locus_tag="VUW50_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7982124.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTNWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSYHRS"
     gene            28046..28645
                     /locus_tag="VUW50_000152"
     CDS             28046..28645
                     /locus_tag="VUW50_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982125.1"
                     /translation="MLSKEEYIEEIGLIEKQNYVEVELYPLVADIINPTLKNSLSKRY
                     VFGRRKSNMGQIYYGLSNFPDIVILDKNYQNKARKSIEIEEWKKLRGCVEIKSLKHDL
                     ITEEKIKSTISNSFEHITGEMGQLIGDLLWYKKVIYTNGIEWRFLSLDDKEEIDNTIV
                     QVVNKRIETEEAGNSFDWWKNIKDLSFNYTDIYLSKDCI"
     gene            28729..28830
                     /locus_tag="VUW50_000153"
                     /pseudo
     CDS             28729..28830
                     /locus_tag="VUW50_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group 2 glycosyl transferase"
     gene            complement(28881..29087)
                     /locus_tag="VUW50_000154"
     CDS             complement(28881..29087)
                     /locus_tag="VUW50_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET7982126.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDILEAVKNCPTRALIGNQEA"
     gene            29139..29615
                     /locus_tag="VUW50_000155"
     CDS             29139..29615
                     /locus_tag="VUW50_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET7982127.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKGFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            29624..30295
                     /locus_tag="VUW50_000156"
     CDS             29624..30295
                     /locus_tag="VUW50_000156"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET7982128.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPQVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            30426..30764
                     /locus_tag="VUW50_000157"
     CDS             30426..30764
                     /locus_tag="VUW50_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HET7982129.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            30767..31396
                     /locus_tag="VUW50_000158"
     CDS             30767..31396
                     /locus_tag="VUW50_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HET7982130.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            31636..32787
                     /locus_tag="VUW50_000159"
     CDS             31636..32787
                     /locus_tag="VUW50_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7982131.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            33105..33854
                     /gene="truA"
                     /locus_tag="VUW50_000160"
     CDS             33105..33854
                     /gene="truA"
                     /locus_tag="VUW50_000160"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET7982132.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQAI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFTVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            33844..34617
                     /locus_tag="VUW50_000161"
     CDS             33844..34617
                     /locus_tag="VUW50_000161"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET7982133.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            34595..35056
                     /locus_tag="VUW50_000162"
     CDS             34595..35056
                     /locus_tag="VUW50_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET7982134.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            35284..36633
                     /locus_tag="VUW50_000163"
                     /pseudo
     CDS             35284..36633
                     /locus_tag="VUW50_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(36762..36965)
                     /locus_tag="VUW50_000164"
     CDS             complement(36762..36965)
                     /locus_tag="VUW50_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7982135.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(36958..38040)
                     /locus_tag="VUW50_000165"
     CDS             complement(36958..38040)
                     /locus_tag="VUW50_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET7982136.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(38166..38948)
                     /locus_tag="VUW50_000166"
     CDS             complement(38166..38948)
                     /locus_tag="VUW50_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET7982137.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(38948..40291)
                     /locus_tag="VUW50_000167"
     CDS             complement(38948..40291)
                     /locus_tag="VUW50_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET7982138.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRYGEIYTTYKMILDAIRKVPT
                     ATVLLNGDSPLFYKPAIPNPVEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(40390..41706)
                     /locus_tag="VUW50_000168"
     CDS             complement(40390..41706)
                     /locus_tag="VUW50_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HET7982139.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVNIFDAEAHFLS
                     NKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            41900..43126
                     /locus_tag="VUW50_000169"
     CDS             41900..43126
                     /locus_tag="VUW50_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HET7982140.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPRVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSASGKEKTDSLTQGMTANQALKS
                     NRFYILWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            43325..44899
                     /locus_tag="VUW50_000170"
     CDS             43325..44899
                     /locus_tag="VUW50_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7982141.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQNIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            45163..45951
                     /gene="codY"
                     /locus_tag="VUW50_000171"
     CDS             45163..45951
                     /gene="codY"
                     /locus_tag="VUW50_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HET7982142.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            45951..46526
                     /locus_tag="VUW50_000172"
     CDS             45951..46526
                     /locus_tag="VUW50_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET7982143.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            46608..46693
                     /locus_tag="VUW50_000173"
     tRNA            46608..46693
                     /locus_tag="VUW50_000173"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46642..46644,aa:Leu,seq:aag)
     gene            46976..48106
                     /gene="ugpC"
                     /locus_tag="VUW50_000174"
     CDS             46976..48106
                     /gene="ugpC"
                     /locus_tag="VUW50_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET7982144.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(48253..48399)
                     /locus_tag="VUW50_000175"
     CDS             complement(48253..48399)
                     /locus_tag="VUW50_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982145.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            48607..49365
                     /locus_tag="VUW50_000176"
     CDS             48607..49365
                     /locus_tag="VUW50_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7982146.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMKLSGMIYDEGWLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            49452..49964
                     /locus_tag="VUW50_000177"
     CDS             49452..49964
                     /locus_tag="VUW50_000177"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET7982147.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            50146..51090
                     /locus_tag="VUW50_000178"
     CDS             50146..51090
                     /locus_tag="VUW50_000178"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HET7982148.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            51099..51776
                     /locus_tag="VUW50_000179"
     CDS             51099..51776
                     /locus_tag="VUW50_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HET7982149.1"
                     /translation="MTYLDAFKSGALVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFIN
                     AMDLWKD"
     gene            51874..52632
                     /locus_tag="VUW50_000180"
     CDS             51874..52632
                     /locus_tag="VUW50_000180"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET7982150.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            52670..54174
                     /gene="lytC"
                     /locus_tag="VUW50_000181"
                     /pseudo
     CDS             52670..54174
                     /gene="lytC"
                     /locus_tag="VUW50_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
     gene            54670..55188
                     /locus_tag="VUW50_000182"
     CDS             54670..55188
                     /locus_tag="VUW50_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET7982151.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            55318..55824
                     /locus_tag="VUW50_000183"
     CDS             55318..55824
                     /locus_tag="VUW50_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET7982152.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            55824..55994
                     /locus_tag="VUW50_000184"
     CDS             55824..55994
                     /locus_tag="VUW50_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982153.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            56026..57258
                     /gene="clpX"
                     /locus_tag="VUW50_000185"
     CDS             56026..57258
                     /gene="clpX"
                     /locus_tag="VUW50_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET7982154.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            57267..57854
                     /locus_tag="VUW50_000186"
     CDS             57267..57854
                     /locus_tag="VUW50_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HET7982155.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            57865..58245
                     /locus_tag="VUW50_000187"
     CDS             57865..58245
                     /locus_tag="VUW50_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET7982156.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            58297..59187
                     /gene="rapZ"
                     /locus_tag="VUW50_000188"
     CDS             58297..59187
                     /gene="rapZ"
                     /locus_tag="VUW50_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET7982157.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            59184..60161
                     /locus_tag="VUW50_000189"
     CDS             59184..60161
                     /locus_tag="VUW50_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HET7982158.1"
                     /translation="MRKPKITVIGGGTGSPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            60158..61069
                     /gene="whiA"
                     /locus_tag="VUW50_000190"
     CDS             60158..61069
                     /gene="whiA"
                     /locus_tag="VUW50_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HET7982159.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHLYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(61103..62071)
                     /locus_tag="VUW50_000191"
     CDS             complement(61103..62071)
                     /locus_tag="VUW50_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7982160.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            62207..63064
                     /locus_tag="VUW50_000192"
     CDS             62207..63064
                     /locus_tag="VUW50_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HET7982161.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYTSTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            63051..63830
                     /locus_tag="VUW50_000193"
     CDS             63051..63830
                     /locus_tag="VUW50_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HET7982162.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            63854..65206
                     /locus_tag="VUW50_000194"
     CDS             63854..65206
                     /locus_tag="VUW50_000194"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET7982163.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            65280..65654
                     /locus_tag="VUW50_000195"
     CDS             65280..65654
                     /locus_tag="VUW50_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HET7982164.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPNELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            65656..66504
                     /locus_tag="VUW50_000196"
     CDS             65656..66504
                     /locus_tag="VUW50_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET7982165.1"
                     /translation="MKLAVFTDSSAYLSAEILQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            66669..67436
                     /locus_tag="VUW50_000197"
     CDS             66669..67436
                     /locus_tag="VUW50_000197"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET7982166.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            67448..68632
                     /locus_tag="VUW50_000198"
     CDS             67448..68632
                     /locus_tag="VUW50_000198"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET7982167.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNLQAITKKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            68629..70500
                     /locus_tag="VUW50_000199"
     CDS             68629..70500
                     /locus_tag="VUW50_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET7982168.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(70643..71827)
                     /gene="mntE"
                     /locus_tag="VUW50_000200"
     CDS             complement(70643..71827)
                     /gene="mntE"
                     /locus_tag="VUW50_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HET7982169.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLATGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLENRF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            72131..74827
                     /locus_tag="VUW50_000201"
     CDS             72131..74827
                     /locus_tag="VUW50_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET7982170.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSVEFATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            74840..75631
                     /gene="yghU"
                     /locus_tag="VUW50_000202"
     CDS             74840..75631
                     /gene="yghU"
                     /locus_tag="VUW50_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HET7982171.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTVGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTEDVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            75747..76157
                     /locus_tag="VUW50_000203"
     CDS             75747..76157
                     /locus_tag="VUW50_000203"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET7982172.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANHDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRV
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            76169..77764
                     /locus_tag="VUW50_000204"
     CDS             76169..77764
                     /locus_tag="VUW50_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982173.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            77831..77995
                     /locus_tag="VUW50_000205"
     CDS             77831..77995
                     /locus_tag="VUW50_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HET7982174.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            78074..78802
                     /gene="yaaA"
                     /locus_tag="VUW50_000206"
     CDS             78074..78802
                     /gene="yaaA"
                     /locus_tag="VUW50_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HET7982175.1"
                     /translation="MKILIPTAKEMNTDLPSIEAISLKPESQAVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTTQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(79079..79627)
                     /locus_tag="VUW50_000207"
     CDS             complement(79079..79627)
                     /locus_tag="VUW50_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET7982176.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            79948..80436
                     /locus_tag="VUW50_000208"
     CDS             79948..80436
                     /locus_tag="VUW50_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HET7982177.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISGSFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQAVQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            80433..81620
                     /locus_tag="VUW50_000209"
     CDS             80433..81620
                     /locus_tag="VUW50_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7982178.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVIDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            81613..81978
                     /locus_tag="VUW50_000210"
     CDS             81613..81978
                     /locus_tag="VUW50_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7982179.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKMAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGIEAGGRDNGVPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            81994..83340
                     /gene="asnS"
                     /locus_tag="VUW50_000211"
     CDS             81994..83340
                     /gene="asnS"
                     /locus_tag="VUW50_000211"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET7982180.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            83493..83783
                     /locus_tag="VUW50_000212"
     CDS             83493..83783
                     /locus_tag="VUW50_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET7982181.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            83795..84265
                     /gene="ssbA"
                     /locus_tag="VUW50_000213"
     CDS             83795..84265
                     /gene="ssbA"
                     /locus_tag="VUW50_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HET7982182.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            84297..84536
                     /locus_tag="VUW50_000214"
     CDS             84297..84536
                     /locus_tag="VUW50_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET7982183.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            84831..86231
                     /locus_tag="VUW50_000215"
     CDS             84831..86231
                     /locus_tag="VUW50_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HET7982184.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFAALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            86233..86592
                     /locus_tag="VUW50_000216"
     CDS             86233..86592
                     /locus_tag="VUW50_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET7982185.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            86646..87395
                     /locus_tag="VUW50_000217"
     CDS             86646..87395
                     /locus_tag="VUW50_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HET7982186.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVVPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            87385..87684
                     /locus_tag="VUW50_000218"
     CDS             87385..87684
                     /locus_tag="VUW50_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET7982187.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            87755..88690
                     /locus_tag="VUW50_000219"
     CDS             87755..88690
                     /locus_tag="VUW50_000219"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HET7982188.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            88777..89289
                     /locus_tag="VUW50_000220"
     CDS             88777..89289
                     /locus_tag="VUW50_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET7982189.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAPGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            89314..89922
                     /locus_tag="VUW50_000221"
     CDS             89314..89922
                     /locus_tag="VUW50_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HET7982190.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKAGLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            90023..90220
                     /locus_tag="VUW50_000222"
     CDS             90023..90220
                     /locus_tag="VUW50_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET7982191.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(90263..91708)
                     /locus_tag="VUW50_000223"
     CDS             complement(90263..91708)
                     /locus_tag="VUW50_000223"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HET7982192.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVRRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            91814..93436
                     /locus_tag="VUW50_000224"
     CDS             91814..93436
                     /locus_tag="VUW50_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET7982193.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            93639..95597
                     /locus_tag="VUW50_000225"
     CDS             93639..95597
                     /locus_tag="VUW50_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7982194.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            95769..96314
                     /locus_tag="VUW50_000226"
     CDS             95769..96314
                     /locus_tag="VUW50_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982195.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDMSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILPLSNISEKYSRRLQEEI"
     gene            96323..96862
                     /locus_tag="VUW50_000227"
     CDS             96323..96862
                     /locus_tag="VUW50_000227"
                     /inference="COORDINATES: protein motif:HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982196.1"
                     /translation="MTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSF
                     ETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTVY
                     SRSKGSSVALDNLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENRPVLKDITCSIFKG
                     QKLLLLDHLDQENQRLCVC"
     gene            96898..97443
                     /locus_tag="VUW50_000228"
     CDS             96898..97443
                     /locus_tag="VUW50_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982197.1"
                     /translation="MEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDNLCLGLNRLV
                     TDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRFLSGGQSQRLQIARTYLKDAEILI
                     FDEATANLDADSEYAIISSLYSVLKEKTVVIIAHSLSTVKDVDCIFFLEEGKITGSGT
                     HKELLENHERYARFVQEQMIE"
     gene            97600..98694
                     /locus_tag="VUW50_000229"
     CDS             97600..98694
                     /locus_tag="VUW50_000229"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET7982198.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLATIESADYALATSSGMSAIVLAFSGFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQAIEG"
     gene            98702..99868
                     /locus_tag="VUW50_000230"
     CDS             98702..99868
                     /locus_tag="VUW50_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7982199.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            99961..103059
                     /locus_tag="VUW50_000231"
     CDS             99961..103059
                     /locus_tag="VUW50_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7982200.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVSDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSELYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            103107..103724
                     /locus_tag="VUW50_000232"
     CDS             103107..103724
                     /locus_tag="VUW50_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET7982201.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            103735..105069
                     /locus_tag="VUW50_000233"
     CDS             103735..105069
                     /locus_tag="VUW50_000233"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET7982202.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            105079..105543
                     /locus_tag="VUW50_000234"
     CDS             105079..105543
                     /locus_tag="VUW50_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7982203.1"
                     /translation="MEIPIKIIQASKFDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEEILLYATSQGYSMIWFNPFYLEEQ"
     gene            105540..106034
                     /locus_tag="VUW50_000235"
     CDS             105540..106034
                     /locus_tag="VUW50_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7982204.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            106115..107770
                     /gene="mltG"
                     /locus_tag="VUW50_000236"
     CDS             106115..107770
                     /gene="mltG"
                     /locus_tag="VUW50_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET7982205.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            107841..108323
                     /gene="greA"
                     /locus_tag="VUW50_000237"
     CDS             107841..108323
                     /gene="greA"
                     /locus_tag="VUW50_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET7982206.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(108584..108664)
                     /locus_tag="VUW50_000238"
                     /pseudo
     CDS             complement(108584..108664)
                     /locus_tag="VUW50_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            108786..109184
                     /locus_tag="VUW50_000239"
     CDS             108786..109184
                     /locus_tag="VUW50_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7982207.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            109642..>109748
                     /locus_tag="VUW50_000240"
     CDS             109642..>109748
                     /locus_tag="VUW50_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7982208.1"
                     /translation="MQNHYTTKGEHLTIDSRRLIERWKKEGKSNREIASL"
BASE COUNT        32543 a        20262 c        24460 g        32483 t
ORIGIN      
        1 tttcagactg tctttaaaac ctttctaaag gacaaagaga aaatcgtcaa cgcccttcaa
       61 ctaccctatt caaacgccaa attggaagcg accaataatc tcatcaaact tatcaagcgc
      121 aatgcctttg gttttcgaaa ctttgaaaac ttcaaaaaac ggatttttat cgctttgaac
      181 atcaaaaaag aaaggacgaa atttgtcctt tctcgagctt agctgacttc aacccactac
      241 agttgacaaa gagccaaaat aaaactttca gggttggtag gggacttgag aaattagttg
      301 attttctcga catagagttg ttttgcaatg tccatattca cttgtaaatc ctcgtcgtta
      361 ctgaggatag tgaaggtatt gctgaagcca tcaaactgct tgacttggag ctggtcaccg
      421 atgtgaagtg agtgcttgtc cagataatgg agaatgtcaa aactatcgtg cacccgagtc
      481 aggcggtagg cgccagcttc cttgatatca gctagtggga ggttattgat ttcaacgagt
      541 agttctccct tggcaggaat agttcccccg tgggggcagg ttttagggaa acctagcagt
      601 ttatctagtc tttccacgaa caggtcagag acagtgtgtt ccaagacctc agcttcctcg
      661 tgaatctggt cacttgtata gtctaaatga tgaactagaa aaacttcaat caagcggtgc
      721 ttacgataga gctcagagac cagtttgaga ccgaggtcag tcagtagata gccacattcc
      781 ttgtccttta ggatgagatt ttcacttttc attcgtttga tcatttcagt tacggcaggg
      841 ggagagactt gcatgcgagc cgcaatttcc ttgttggtaa tcttatgcag gtctatgcca
      901 atttcataaa tacattttag atagtcttct ttgtttgggg tcattcgttt cctcttgtct
      961 aatatcttta tctagtatat caaaaaaagc tagatttctc tagctgtgac tttttatgtt
     1021 atacttattg caagagaaaa aacgaggaga tggatatgac gaaaatagct cttctttcag
     1081 atattcatgg aaataccacc gccttggagg ctgttttggc agatgctcgg cagataggag
     1141 tggatgaata ctggcttttg ggagatattc tcatgccagg gacagggcgt agaaggattt
     1201 tggacttgtt ggatcaacta ccgattacgg ctagagtttt gggaaactgg gaagacagtc
     1261 tttggcatgg tgtccgtaag gaattggata gtactcgccc cagtcaacgc tatctcttgc
     1321 gccagtgcca gtatgtttta gaggaaattt ccctagaaga aattgaagtg ctccacaatc
     1381 aacctcttca aattcatcgt cagtttgggg atttgacggt gggaattagc catcatctgc
     1441 ctgataagaa ctgggggcga gagttgattc atactggcaa acaagaggag tttgaccgct
     1501 tggtgactca tcctccctgt gatattgctg tttatggaca tattcaccag cagttgcttc
     1561 gttacgggac tggtgggcaa ttgattgtca atccgggttc gattgggcaa cctttctttt
     1621 tggacgctca gctacggaag gacttgcggg cccagtatat gattttggag tttgatgaca
     1681 aaggcctggt agatatggac ttccgacggg tagactacga tgtggcagct gaattgcagc
     1741 tggctaaaga cctgagactt ccctattttg aggtttacta tgaaagtctg gtcaatggga
     1801 tccaccatac tcatcatcag gaatttttga gagaattggc tcagaaagag ggctgcgatc
     1861 gggagttaga cgactggttg aaaagtggta acgattgaca ttacaactaa aaagggtata
     1921 ataatagaaa aagaagagta gaggcagggt agcctcgtaa aaaggagaag aggatgcaaa
     1981 ttccaagtag atttaccatt gcgactcata tgctgataat cattgccctc gaagggaagg
     2041 aaagcaaggt gaccagtgat tttctggctg ctagtgtcgg ggtcaatcct gtcattatca
     2101 gaaagatctt gtcccagttg aagaaggcag agctgatttc agtagcgcgt ggaacgggcg
     2161 gaacagagat tgtcaaggac cttaaggata ttagtctttt agatgtttat caggcggtag
     2221 aatgtcttgg taagacaggt caactcttca gtttccatga caatccgaat ccaaattgcc
     2281 ctgtaggagc tcatattcat gatgttttgg atcaaaaatt ggagagaatt cagttgacta
     2341 tggaggcaga acttggtcaa accagtctag aaaaagtcgt ggccgatgca gagagtcaga
     2401 tgaaggatta agagggataa atgccctctt tttttctttg gataattatg ataaaatgta
     2461 gctattaaag gagtaagaaa tgtatctcgt tataggaatt gtattagcct ttatagtgtc
     2521 attttggaag tataatagaa gtttgtggaa tccagtatta tttttattat ccctcatttc
     2581 aagttatttt tatctatcct acctttttta taaaaatgga tacgagaatg ttcagttagc
     2641 tttttatgta tttgcctttg tattacttcc tttttttgct ttttctaagt ggcatctttt
     2701 taatctataa tggggtcatt ttgttgaagc gagagggacg ttccaagccc cattatctct
     2761 caatgctctt tgatttttat tgactattag attctattta aaaagactta gaatgcacat
     2821 gaaagtatgt attctaggtc ttttgttttg tatacattta attttcaaga gtattatcta
     2881 tttttccata cctggtaacg agtatcaatc aataactggg taaggcgacc caaggtttct
     2941 ttttcttctg gagtgaggga ttgagcttgg acaaagagat gatgaatgta ttcaagcgcc
     3001 tttaaggaag ccaagtcatt gatagagcta ataccagcat caagaatagt gccaaagaag
     3061 aggtcgaagt agagctgaag ttcttcgtca gggactgtgg tcacccattc tttaaaggtt
     3121 gtgtctactt gccggctatc actgttggtc ttatccagtt gaacgaagtg cttatcctca
     3181 atctgccaac taaaggtatc gtgctgggcg atgccaccca gggcagtact ctgaacgatg
     3241 atttggtgag caggaatttc cagcatcata ccgataatgg aaccttgtgg aatgaatatc
     3301 ttgcttctat ccattatcct ttgataaccc gcagtctgtg tcaattcttg atggagacca
     3361 ggtgcatcaa atgtataaac tgctgtgatc tgattttgca aactttgctc aatttggcta
     3421 gcagcataga tagcgagatt tcctcccttg gaatgcccag ccagaataac cttttgctta
     3481 ggatgatggg caaaaaagtt ctttaaatag cgaagggcgt gcttttgagc aggaatttcc
     3541 ttcatatagg tcaggtggaa atcttccttc cagccaatga tactgtcatc tgtcccacga
     3601 aagacaatca gataggtatc gaggctgaca cgataagtca tagccgcaaa ttgcttttgc
     3661 agttcagggt cgatgtcgtt gataaaatgg gagagtttgc aatttttgaa gcgcttgtgt
     3721 tgagccaatt catctaataa ttgaaggcga tttttgctag taagcatggt gggctctctt
     3781 ggaacctgag gtgctagatc taaaagacgt tgaggaagtg tggagaccag attatcaaag
     3841 gagaggtagg tgatttctat taaggttaga atgtctaact cattcaaggg aaggtcgtaa
     3901 taagaatcat atgcgacatc tttcagatag tcaaaaatat tggccataag agctcctttc
     3961 tttttattta tcttagcaaa tttccctcaa attagctagt agcatagcct gtttgtactg
     4021 gctaaaaaca ggctatttca aattcagttt cagaccatct agcatggaaa aatctgttat
     4081 aataatggaa aaggagaagc gcatgcacaa gattttatta atagaagatg atcaggtcat
     4141 tcgtcaacag attgggaaaa tgctctctga atggggattt gaagtggtcc tggtagaaga
     4201 ctttatggaa gttttgagtc tatttgttca gtcggaacct catctggtcc tcatggatat
     4261 tggtttgccc ttgtttaatg gttatcactg gtgtcaggaa atccgcaaga tttccaaggt
     4321 acctatcatg tttctttctt cgagagacca ggctatggat attgtcatgg caatcaatat
     4381 gggggcggat gactttgtga ccaagccttt tgaccagcag gttcttttag ctaaggttca
     4441 gggcttgttg cgtcgttcct atgagtttgg gcgtgatgag agtttgctgg aatatgctgg
     4501 tgttatcctc aataccaaat ccatggattt acattatcaa gggcaagtct tgaatttgac
     4561 caagaatgaa ttccagattt tacgcgtgtt atttgagcat gcaggcaaca tcgtagcacg
     4621 tgacgacctg atgcgggaac tttggaacag tgactttttc attgatgata ataccctctc
     4681 tgtcaatgtg gctcgtttgc gtaaaaagtt ggaggagcag ggattggtag gatttatcga
     4741 gaccaagaaa ggaatagggt acggattgaa gcatgcttga ttggaaacaa ttttttctag
     4801 cctatctgcg ctcccgtagt cgtcttttta tctatctgct ttctttggca tttcttgtct
     4861 tactctttca gtttttattt gccagtctag gaatttactt cctctacttt ttcttcttgt
     4921 gttgctttgt aaccatatta tttttcactt gggacatatt ggtggaaacg caggtctatc
     4981 gccaggaact tctctatgga gagagggaag ccaagtctcc tttggaaata gctttagcag
     5041 aaaaattaga agcgcgtgag atggaactct atcagcagag gtcaaaagca gaaagaaaac
     5101 tgacggattt gctggattac tataccttgt gggtccatca gataaagacc cccattgcag
     5161 ccagtcaact cttagttgca gaagtggtcg accgccaact gaagcagcag ctagaacagg
     5221 aaattttcaa aatcgactcc tataccaacc tagttttaca gtacctgcgt ttagaaagtt
     5281 tccatgatga tttggtctta aagcaggttc aaattgagga cttggtcaag gaaataattc
     5341 gtaaatatgc tcttttcttt attcaaaaag gcttaaatgt caatctacat gaccttgata
     5401 aagaaatcgt gacggataaa aagtggctgc tagtggttat tgagcaaatc atctcaaaca
     5461 gtctcaagta caccaaggaa ggtggtctgg agatttatat ggatgaccaa gagctttgta
     5521 tcaaagatac gggaatcggg ataaaaaaca gtgatgtcct ccgagtattt gaacgtggct
     5581 tttcaggata caatggccgt ttgacccagc agtcctctgg acttggcctt tatctatcta
     5641 agaaaatttc tgaagaactg gggcaccaga ttcgtatcga gtctgaggtc ggaaaaggaa
     5701 cgacagtgcg gattcagttt gctcaagtga acttagtcct tgagtaaaaa tagatgagaa
     5761 aaagcctcca gattggaggc ttttatgcta gactcttatt tctcaacgcg tgatttgttg
     5821 gcgatatcag ctaggatagc ttgaacaaaa ttatcaactg agacagtttg tgtttctttt
     5881 tggccgtagc gacgaacgtt gactgtttcg tcttccattt ctttgtctcc aacaattaat
     5941 tggtaaggaa tcttgctggt ttgtgaagca cggatcttga actgcatttt ttcattgcgc
     6001 tcatctacgt ctgcacggac accgcggtca cggagtttct tggccacttc ccaagcgtag
     6061 tccacgtgtt tttcgttaga tactgggatg agggttactt ggtgtggtgc cagccatgtt
     6121 gggaaggccc ccttgtagtt ctcaatcaag atagctgtga agcgttccat agttgaaata
     6181 accccacggt ggatcatgac tggacggtga tcttcgccat cagctccgat gtatttgagg
     6241 tcgaagcgtt ctggcaacaa gaaatcaagt tggatagtag aaagggtttc ttcttttcca
     6301 agggcagttt taatctggat atccaatttt ggtccgtaga aggctgcttc accttcggct
     6361 tcaaagtagt ccacgcccat ttcatcaaga gctgcacgaa gcatggtttg ggcattttcc
     6421 cacatctcat cgttatcaaa gtacttatga gtatcttgag ggtcacgaag agagaggcgg
     6481 aagcggtagt cagtcaagtt gaagtcttca taaacatcga taatcaactg aagggcacgt
     6541 tggaattctt cttggatttg ttctggagta acgaataggt gaccgtcgtt gagtgacatt
     6601 tcacgtacac gttgaaggcc agtgagggca ccagattttt cgtaacggtg catcataccg
     6661 atttcagcga tacggattgg caattcacgg taagagtgaa catggtgttt gaaaacttgg
     6721 atgtggtgcg gacagttcat tggacgaagg acaaattctt ccccgtcacc catgtccatg
     6781 gttgggaaca tgtcttcttg gtaatgatcc cagtgaccag aagtcttgta aagctcaaca
     6841 gaagcaagtg gtggagtgta gacgtgttgg tagccagaaa ccaactcttt gtttacgatg
     6901 tagcgttcca attcacgacg gatagtcgca ccatttggca accagaatgg caaaccttgt
     6961 cccacttctt gtgaaatcat aaagaggtca agctctttac caagtttacg gtggtcacgt
     7021 tccttagctt cttcacgcat ttgaaggtag tttttcaagt ctttcttgtc aaaccaagct
     7081 gtaccgtaga tacgttgcat catagcgttg tcgctgtttc cacgccagta cgcaccagct
     7141 acatggagaa ggtggaagat ttggatacga cctgttgatg gaacgtgagg tccacggcag
     7201 aggtctacat attcaccctg acgatagata gtcaaaccgc cttcgtcttc tgagtgttct
     7261 tcaatcaatt ccaacttgta agggtcattt ttgaagattt cacgtgcctc gtctttagtc
     7321 acttcttcac gaatagatgg gaagttttct ttgacgattt tttgcatttc ttcttcgata
     7381 cgaggaaggt cttcgttaga gatttgacca gctgtgttgt cagtatcgta gtagaaacca
     7441 tcttcgatgg ctggaccaac tcccaagtga atgtctggga aaagacgacg agctgcttgg
     7501 gcgaacaagt gagctgctga gtgacgcaag attggaaggg catcttcgtg atcaggtgtc
     7561 acaatttcga tgcttccatc ttcagtgata gcgcgagtag tgtcgatgag tttgccgttg
     7621 aatttaccag ccaaggcttt tttagctagg gaattgctga tagattgggc aatttcaaaa
     7681 gttgttacgc cagattcgaa ttcacgaaca gcgccatctg ggaaagtaat gttaatcatg
     7741 atgttctcct tttttattat ttcatagttt ctaagcattg attttttatc agcagactac
     7801 ttccaagtcc taacgttcaa ttgcttttct ttatcagaga ggaagttttc catttccgtc
     7861 agaatgtcta tatccaaacg ttgagaaagt tctaatagcc accagatatt ttctgaaagt
     7921 ttttgttcca gtgtgtaggg tgtttcatca tagtagcgtc cttgctttgt catcaccagt
     7981 cgttggaaat ttccaatatc attagataaa gccaagaggt cttcttctac cgtccacttg
     8041 gaatcatgat gcttaacttc cagttcgtga taagcttgtc ggattgccca actgcgctcc
     8101 actaattctt gcaactccat aattagtatc ctaataaaaa aatgcgacat cctcgaaagg
     8161 acgtcgcaac gtggttccac cttcatttat gtacctcaaa agagagggac acctctgatc
     8221 ggctctaacg tggccaccgt tttatgtttt cataaaaaac tcaagagtag tatcagttta
     8281 gacttcctat gcgtttccag caaccacgca ctctctagta gaaagggact aagtgacttg
     8341 tctctatgga ttattatagc atgattgtga gatttttcaa ggattttgtg aaagtttttt
     8401 gtttttcatt ttttgtagaa tatgcttgat gctaatacca gaatccatgc cgaccagagc
     8461 aactgtttca tagattagca agtaaattag tataaactca ccaaaggtta tgatagtaaa
     8521 gtaaaagagc agtccgttac caactaacca tcaaagtgag aaacggaaac ggtagctata
     8581 gagtaacgat ataatcaaga ttaccagagg tgtaaagaag agaagattgt ttatatagag
     8641 ttccttgagt gtaagggggt cagaaacaac tagtaagaac aactcataaa gtggccaata
     8701 aaagaataat atgaccagat agtaggggag atgggaatag aatctacgca tgataatcac
     8761 ctcgttttaa aaaaacagac aacttagatc tcttgctatg attattatag catgtttaaa
     8821 acaagaaagt aaatttgttg acaaagaaga tttttctttt cactagaatc atggttagtt
     8881 ttcttggtag aaatattttg ataataatta agcaagacta ttgacattga ttattaagag
     8941 tgtcacaatt gttgtaacta aaataattac aacaagagag gtataaaaaa tgaaactagc
     9001 agttattgca gcaaatggac aagcggggaa agcaattgtt gaagaagcag tcaaacgagg
     9061 acatgaagta acggctattg ttcgttctga aaacaaaagt caagctgagt ctattattaa
     9121 aaaagattta tttgagttga caaaagatga tcttactgga tttgatgctg ttatttcagc
     9181 gtttggtgct tatacaccag atacgctacc gctccacagc aaatcaatcg aactatttaa
     9241 tcagcttctt gctggaacac agacacgttt tctagttgta gggggagcag gaagtcttta
     9301 tatagatgaa acaaaaacga cacgactttt agatacgcct gattttcctg aagaatttaa
     9361 gccactagca aaagctcaag cggatgaatt ggatcttctt cgtacaaaaa ataatctcaa
     9421 ctggactttt gtcagtcctg ctgtagattt tattcccgat ggtgaaaaaa caggaaacta
     9481 cattctagca ggagaaattt ttactactaa tgaaaaagga ataagccaga ttagttatgc
     9541 agattatgct attggattgg tagatgaact tgaaaaaggt catcatataa aagaacgtat
     9601 ctctttactt gaaaaataat aactatgaga gtattaatgt ttgacagaaa atttaaatct
     9661 tggtaaaata gggattgtcg taaagacaaa taacttcttc ttggttacag gcatgccaac
     9721 ctgtcactcg gatgaagcca aataaaaagg agaaacatca tggcaatctc aaaagagaaa
     9781 aaaaatgaaa tcatcgcaca atatgcacgt cacgaaggtg atacaggttc agtagaggtt
     9841 caagttgctg tccttacttg ggaaatcaac caccttaacg aacacatcaa acaacacaaa
     9901 aaagaccacg ctacttaccg tggattgatg aaaaaaatcg gtcgccgtcg taacttgctt
     9961 gcatacttgc gtaaaaacga cgttaaccgt taccgtgagt tgatcaactc tctaggactt
    10021 cgtcgctaat tcaagataca aaggccgtca aaagcacaaa gcaaaaatag gaaaattgac
    10081 gaagaaactt cagtttctag gagattttat cttttttgcc aagtgcttag gccgtgttca
    10141 attgagcata tcttgataat aaagctactc taaattgggt agcttttttg cgttgtcacc
    10201 ttacctcgat atactcaagt ataatctttg gttacggttc ctagcactgt aaggtaaaat
    10261 aaaccaactt ttctcccttc ggggagattt tttttgtttc actgaaagtt tcgtacgatc
    10321 ttcgcctcgt cgcctagtct ataaacgatt tatctagcaa gaatcatgat gctaagggcg
    10381 tcaaaaatcc gtatgaaaat agggaaatga cacagtgttc gatgagcaca aggagtttca
    10441 tctttttcac taggatttta gcccgggctc aaatcagctc tctgacttca gagggctttt
    10501 ttattgaggt tttatcggta acaattttgg agactacaaa aacctcaaac ggtatcagct
    10561 aattacacca taaaggtgcg cagttactcg gcttttcaag ccgagtagct gtatgcaagc
    10621 cccctagaag agcccactct ttacaaagta aagtatagta tgttatactt tacatggaag
    10681 tagtcactga attccagtta gaaattactt tgtaactacg ttttgaggag gagtaaaatg
    10741 ctttcctacg ttcgatatta cccactagcg atagctaaat taatgtgtct gtgctctcct
    10801 aaaatctgct gatttattac tgactaatac aggaggtttt ttttatggga cagacaatca
    10861 tatctgctat tggtgtttat atttccacca gtatcgatta tttaattatt ttaattattt
    10921 tatttgcaca gctatcacag aataaacaga aatggcatat ttatgcgggg caatatctag
    10981 gcacaggctt acttgtaggg gcgagtttag ttgctgctta tgtcgttaat ttcgtgcctg
    11041 aagaatggat ggttggattg cttggtttaa tccctatcta tttagggatt cgctttgcaa
    11101 ttgttggaga agatgcggaa gaagaagagg aagaaattat tgaaagatta gaacaaagca
    11161 aggcaaatca actgttttgg acagttacat tgctgacaat tgcgtctggc ggagataatt
    11221 taggtatcta tataccttat tttgcttcgt tagattggtc acagaccctc gtggccttgc
    11281 ttgtgtttgt aatcggcata attatctttt gcgagattag tcgggtgtta tcctctattc
    11341 cgttaatatt cgagacaatt gaaaaatacg agcgaatcat tgtgccctta gtattcattc
    11401 tacttggact atacatcatg tatgaaaatg gcacgataga gacttttctg atcgtgtaga
    11461 tttttttgtt tcactaggat tttagcccga gctcaaatca gctctctgat tttcagaggg
    11521 ctttttcatt gagtataaat ggacaatctt aaagttactc tattgtagag tggcttttga
    11581 tttgattagg agattcttat aaatatagta aaatgaaata agaataggac aaatcgatca
    11641 ggacagtcaa atcgatttct aaaaatgttt tagaagtaga ggtgtactat tctagttcca
    11701 atctactata taaatgaaaa ccttagctgt ttggccaagg ttcatttctt ctatgcaagt
    11761 tcttctcttt tcttatctgg gttccagatg aagcttgcga taaagctaaa gatgatggtt
    11821 aggatagcga ggataatagc aaggatgagg gcagggatgc ggataaacca ccagagtgga
    11881 tttggttttt tatcattgtg ccattgtttc gtttcttcgt ccagacgttg gaattctgtt
    11941 tgaatacgat tggcgactgt ttcaatgcct tcatcattca ttttcttgat atctgagata
    12001 tcgattggat ttccaaagtt catatcgaca cgttcacggc taatcaagcc cttcaaagtc
    12061 atgggaccgg tgtaggtaac cggcatgata cggaccttgg ccattttggc aatcagtgct
    12121 gcgcccccct tgacatcgtt tgagtggcgg ctaccacttg gaaacatgat gagagagcgg
    12181 tcactttttt tgagaacgtt gataggatat ttgatggctg aggcgctggg attttcacgg
    12241 tcgatgggaa aggcgccaca catacgaatc caccaaccaa agatacggtt ggtaaagagt
    12301 tctttttttg ccataaagat gaactgtttt ggcttggtcg caaaggccat ataaacagga
    12361 tcccaccagg tacggtgagg cgcaactaaa atataatttt catcttgatt aggaatttta
    12421 tcagtattat gatagtgagc attgccattg atggaccata agagcaatac aactaatcca
    12481 cgcaaataag tataaaacat gcgatctcct tcgattgttt tcttgttatt attatacctt
    12541 atcaaaggag ggctggcaaa cttttccctt gactagatgc atatttggga tgaaattaga
    12601 attctgttaa aaaaaatgat ataatagaat ttatggataa aaataagatt atgggattaa
    12661 cccaaagaga agtcaaggaa agacaggctg agggtttggt caatgacttt accgcatcag
    12721 ccagtaccag cacttggcaa atcgttaaac gaaatgtctt tacccttttt aacgctttga
    12781 actttgccat tgctttggcc cttgcctttg tgcaggcttg gagcaatctg gtcttctttg
    12841 ctgttatctg ctttaacgct ttttctggga ttgtgaccga gctacgagcc aaacacatgg
    12901 tggacaagct caatctcatg accaaggaaa aggtcaaaac catccgtgat ggtcaggaag
    12961 ttgctcttaa tcctgaagaa ttagtgctag gagatgtcat tcgtttgtct gcaggagagc
    13021 agattcctag tgatgccttg gttttggaag gctttgcgga agtcaatgaa gccatgttaa
    13081 cgggagaaag tgatttggtg caaaaggaag ttgacggctt acttttgtca ggaagtttcc
    13141 tagccagtgg gtcagtttta tctcaagttc accatgtcgg tgcagacaac tatgctgcca
    13201 aactcatgct tgaggctaag accgttaaac ccatcaactc ccgtatcatg aaatcgctgg
    13261 acaagttggc tggttttact gggaagatta tcattccctt tggtctggct ctcttgctgg
    13321 aagccttgct tttaaaaggc ctgcctctca agtcatctgt tgtaaactcg tcgacagctc
    13381 ttttgggaat gttgcctaag ggaattgccc ttttgaccat tacttcgctc ttaactgcag
    13441 ttatcaagct aggtttgaaa aaggtcttgg tgcaggagat gtactctgtt gagaccttgg
    13501 cgcgcgtgga tatgctctgt ctggacaaga cgggtaccat cacccaagga aagatgcagg
    13561 tggaggctgt tcttccgttg acggaaacgt atggtgaaga ggctattgcc agcatcttga
    13621 ctagctacat ggcccatagt gaggataaga atccaactgc ccaagccatt cgccagcgtt
    13681 ttgtgggaga tgttgcttat cctatgattt ccaatcttcc cttctcgagc gaccgcaagt
    13741 ggggggctat ggagttagaa ggtttgggaa tagttttctt aggggcacct gagatgttgc
    13801 tggattctga agtcccagaa gccagggagg ccttggagag aggatcacgt gtcttggtct
    13861 tagctctcag tcaggagaaa ttagaccatc acaaaccaca gaaaccatct gatattcagg
    13921 ctctagcctt gctggaaatc ttggacccca ttcgagaggg agcagcagag acgctggact
    13981 atctccgttc tcaggaggtg ggactcaaga ttatctctgg tgacaatcca gttacggtgt
    14041 ccagcattgc ccagaaggct ggttttgcgg actatcacaa ctatgtagat tgctcaaaaa
    14101 tcaccgatga ggaattgatg gccatggcgg aggagacagc gattttcgga cgtgtttccc
    14161 ctcatcaaaa gaaactcatc atccaaacgt tgaaaaaagc gggtcataca acggctatga
    14221 caggggacgg ggttaatgat atcttggccc ttcgtgaggc ggattgttct atcgtgatgg
    14281 cggaggggga tccagcaacc cgtcagattg ccaatctggt tctcttaaac tcagacttta
    14341 atgatgttcc tgagattctc ttcgagggtc gtcgcgtggt caataacatt gcccacatcg
    14401 ccccgatttt cttgataaag accatctatt ccttcctgtt agcagtcatc tgtattgcca
    14461 gtgctttact aggtcggtca gagtggattt tgattttccc cttcattccg atccagatta
    14521 ccatgattga ccagtttgtg gaaggtttcc caccattcgt tttgactttt gagcgaaata
    14581 tcaaacctgt cgagccaaac ttcctcagaa gatccatgct tcgtgcccta ccaagtgccc
    14641 tcatggtcgt gtttagcgtt ctttttgtga aaatctttgg aacgagtcaa ggttggtctg
    14701 agttagaaat ctcaactctc ctctattatc tcttggggtc aattggtttc ttatccgtat
    14761 ttagagcctg catgccattc actctatggc gagtcctctt gattgtctgg tcagtaggag
    14821 gcttcctagc cacagctctc ttcccaagaa tccaaaaact gcttgaaatt tcaaccttga
    14881 cagaacaaac gttgcctgtt tatggtgtca taatgttggt ctttacagtg attttcatcc
    14941 tgactagtcg ctatcaagct agaaaataaa gaaagactgc aatctgtgga ttgcagtttt
    15001 ttatacgatg gatctatcgt agatctgatg tgcaaggcct acgtgtctat tatctatcgg
    15061 tgaacccaag agtgattctc aagcctgctt ggattgaggt aatagattca aatatctgta
    15121 gttagactat ttgaagtttg atgtaagaaa taattctctt cgaaaatatc ttcaaaccag
    15181 gtccgttcta tccacaatct caaagcggta ctttgagcaa cttgtggtca gctccctagt
    15241 ttgatagtat aaaaactgtc agataaaggg gtggcgaaat tgtatctaaa cgaacagaaa
    15301 cgtgatggta aggcgtatag agctcatagg atggcaagga aagtctagaa cctaaaaaag
    15361 aactagataa atccactaag tgtggttact ctagttcttt ttcgtttatt caacacccaa
    15421 gatttcttta atatcatcaa cgctcagatt gcttctgctt tcttgtcctt caaggacggt
    15481 gttgaagagt tcttttttac tttcttggag ttcttgtatt ttttcctcta tggtaccaag
    15541 tgtgataagg cgatagacat cgacttgttt ggtctggcca aggcggtggc ttcggccgat
    15601 ggcttgcatc tcaactgctg gattccacca gaggtcacag aggatgacgg tatcagctcc
    15661 tgttagattc agtcctgttc cacctgcttt tagggaaatg aggaagcagt ctttttcccc
    15721 ttgattgaaa agattgacca tttcttgacg tttatctgaa ggtgtttgtc ctgttatttt
    15781 aaagtgagac atttcttttt tctctagttc ttgctcaatg agatcaagca tagaggtgaa
    15841 ttgtgaaaag ataagtggtc gtgacccttt ttcacgaatg gtgtctagga gctcgaataa
    15901 gctgtccatt tttccactat cccctttgta gtcttctaaa aagagagcag gggtattaca
    15961 gatttgtcta agtcgggtca gtcctgctaa gatttcaatt ttatgacgtt taatctcata
    16021 ggcgctggct cctgaaacca tttcttgcat gcgtcgaagt tgagctagat agagcgtttt
    16081 ttgcgaactg ctgagttcat tgctgtagag gtgctcgctc agttcaggca attctgtcag
    16141 aacttcgtct tttttcctac gcagaacaaa aggttgaatc agtttagcaa cgagttgagg
    16201 tgacaatttt gaaaattcct ttttacttgg taggagacct ggtaatacta tctggaaaat
    16261 agaccaaatc tcttcgagac ggttttcaat aggagttcca gatagggcat agcaggtttt
    16321 gacatcaaat tctcttaaag cttgtgctgt tttggtctga ctatttttta tcatctgtgc
    16381 ttcatctagg atgaggtagt cgtaagactg cttttgataa tgttcaaggt cagatcgaaa
    16441 agatggatag cttgtaatag tgatagtagc agatttttca atttgtttga tacgctcagt
    16501 tttgctacca taggctactt caacctgttt gtgaggcaca aattttcgga attcttcctt
    16561 ccaattataa agaaggctgg ctggagctag gataagagct ttctggtctg gtttaaggtt
    16621 ggcttccaga agagtgatgg tttgcaaggt tttccctaga cccatgtcgt ctgccaggat
    16681 tcccccaaaa tgataatgat ccagcatact cagccacttg actccttctt tttgatagga
    16741 gcggagtttg gccttgactt cataaggctt gatatcaaat tcttcagggt gggtcaggtc
    16801 ataagccatc tttttgaaat cacgagaaaa attgacatgt tcattgtctt tgaaggtttc
    16861 agaaaggcta aaggcacgag atttgtgcat cttgattttt ccgtctgaaa atttagcgcg
    16921 aagatcttgg agactttgag caaccttgag actttcatcg tcaaagacta gaacctttcc
    16981 ttgcttggtt tggtagtggc tttcttgatt ccataaagct tggatagctt tttcaacctc
    17041 atcttctgaa atcatagaaa aatcaaactg aactgaaagg aagctttcgt cgtcaaaaat
    17101 gtcaatgaca gcaggatctt ctactaagag tttttcaagc gattcatcta gttgaacttg
    17161 acccagagta cgaaaacggg ggagttcttt taggaagaaa tcaacgattt tgattttgga
    17221 aagactatga aattcctctt tgaaaccagc agaaagcagt tgatgataga tttttctagc
    17281 tttgctagct tggtaagtat aggggagggt taggagatct tgtttagaat ggacttgttt
    17341 ttctccaaag gaccacacca tattaagggc aatttcattt ctagtcttgg agaaggcaaa
    17401 ggtggggata aagtcatgga tttgtaattc atctgggata ctgataggaa catagtttga
    17461 gatgatatta cagacttgca tgagttcagc cttattttct tttgaaaaga taagaggaga
    17521 attttcttga taatgataga tccaagtagc taatttatcc aaaaagtaac ggtgttcttc
    17581 tttgatgatg tggaagtgat tgtgtcgaat ccaaagtttt tcttgaataa tgaaaccaaa
    17641 ataagctgta ttgatgattt cataatggtt attagatttg acaactttaa aaatttcctc
    17701 ttccgggata aagggactat catctaagaa tataagaggg tattcggctt ttgaggttac
    17761 atattggaag acatccaaag cttgaaaaag tgggacgagg tcgtttaaat agctaacagg
    17821 agtctctaaa tagcgtccgt aatctctagg gttaatgcca taactgaagt tgaaataaga
    17881 atgttcattt ctatcgataa aatatttttt aatatagagg agtaggtttt gggatgcttg
    17941 attaaactga ttcaaggata atctgattgt cccgattttc tgtgaaacaa agtaatcttc
    18001 cttttggaaa atgagaaaaa tgaagtgagc tatatccttg ataacatagg cacgaggagc
    18061 ttgttctacc tgtaatttta aggtccaggc taggctatga tttcggattt cgacttgtcc
    18121 atgaagggca agtgaaggcg ccgtagcttt ttcttctacc agttcatctt ggagctcttc
    18181 agatggcaag gtaggaatca gttggctggc aaaacttggt ttatgactcg ctttaccaat
    18241 gcgttcttga atagcttggg ggagactacg aaaataaaga tccatggcaa cccagtgttt
    18301 acagactccc tgattttgga aatccttaca ttcgcaatga aagccagtca aatcgaagtg
    18361 ctccatactg gtcaggatgt tgggctccag atgcatggaa ataccatcat aggtattggg
    18421 gtgcttaaaa tattccttat tttcagtatc aaaatagttt tttatctgtt ggaaaataga
    18481 gcggccatcc attctcatac gagcaggtag tctatcggga accgtcattg aagtaacctc
    18541 tgttctaatc attcttttct ctatcttatc agaatttata ggaaaagaat agatttatct
    18601 tacataaatt gcatcttgat agggattgga aacatttgta gttgcaagat agttgtatgt
    18661 aatatcgaca atcaattaca aggagattac gattccatag aaactatcaa gtgaaaactg
    18721 acttatccat aacattctag ctaaacttct tcttggtttg gtaagttttt catctggtga
    18781 actagatagg ctgctataag tagtaaaatt ataagagcaa aagcaacaac ttccagacca
    18841 gcacttgttg caagaaatac gagagcaagg gataggacag ctgggataag taagttgata
    18901 acattgatgg atgattgaac tgatcccatt aactcctcag gaatgattcc aaaaatcatg
    18961 gcttgcaagc gaggactcaa tactcctgcc gagaaagcat cacccgcact tcctagtaaa
    19021 ataagtaaaa aacttccacg tagaaaatct ataacaatga gagactccat caatagactc
    19081 caataaatat tcaggcgaat agaaattttt ttagatagaa atccactcag aagtccacct
    19141 aaaataacat gaagaaggct tatcattatt aaaattgcca gactttgccc tgttgacaaa
    19201 cccataaaag gatggtgaat cagtagaatc gcactgacag gctctaccat catcaaaaca
    19261 gcttgactaa tggacaaaat ccaaagcaag accatgacat cttccatagt catcaataat
    19321 ttcattgact ctttcaaatg ctggaaaaag ctactcattc ctacataagg aggaacttcg
    19381 attttttctt cctctttttt caagcggttt cggataagta gacttcctag aaaggcaaat
    19441 aaaaaggtta aaacatttac aaaagctaga gtagaaatgc taaacagttc taaaaatact
    19501 cctccgctga gattacctat caaacgtaca atggaactcg tggactgcct aaaacccata
    19561 gcctccgtca tatcatcgtt tattagtcgt atgtaaatcg gtgtcagcat gtatcctgca
    19621 agaaaaccaa tcgtatctga aatcaagttc atgctgcaaa taaagaaaac agaaaagaga
    19681 gagaagtcgg tacttgtcag gagcaaagtc actccagaat aaaggaggac cttgataaag
    19741 aggatagaaa tatacttgct gattcgattt ttttgaaaat cagctataac tccagtaaaa
    19801 agttgcaaga cctgtggaat ggtttctgat aggctaatca aaaatatagc taagggagca
    19861 aaggcataag aagagacata attgataaaa gctaagtaaa aaatagtatc tccaaaactt
    19921 gaaatccact catttactgt tagctgacga aaagctcgat ttttaagaaa aatatgcata
    19981 ttaatctcct ttacttaatt caaatgggaa tgtataaccg agtaaagtcc aataaatacc
    20041 cagatttccc acaccaaagt caaaataact gtttattcca tcttcccctt gaaattgtaa
    20101 taatctatca ttattacgat aagaacgagt caataagatt tttttttatg aactgttcaa
    20161 ccttttgatt tttattgtat attgttgttt gcaataaact acttaaacca tggcaaaaac
    20221 tagttcccat tgcttcatat tgttgcacca tttcaagttg tgatttatag attatctcag
    20281 agtatctttc tttatcaatc aaacaaagat acaatattaa tcctgaaact ccgtcacacc
    20341 aagaaagacc taaattatct aatttagaaa agcttgaaag tttctcttct agtaattcag
    20401 atatcccgtc agaaaatttt tgtaaatgat tttgatagcg actatctcca aaaatatcag
    20461 tataggcttt taatacatag gcagcacctg aacgtccgtg agcaaaatta ttagaatata
    20521 tatctccatt atcctcggat tctaataaac tatatgcatc atttatattg ttcttgataa
    20581 aatttgctag ttttttatca tcaaaaatgg tacaatattt catcaaactg agtaggatac
    20641 cagacttccc taaagcaaaa tctaagttac taatattgtc ataaacccgc attaaatgat
    20701 cgactaatct cttacttatg tctattaaat atctctcctt tgttttttca tacctatcta
    20761 ggattccaaa taggaaacct gctttcccaa atagtagaga gtaaccatgt aaaaaattag
    20821 ctgcttcata attttctaat ttcgtaagcc attctttaac ggtattttca tcttcctcaa
    20881 cataatattt attcataaaa aataaaactc ctccaaaacc atgttggaaa gaaacaggac
    20941 ttacgaactc accaaattca tttgacggaa acaaacgacc agatttgtcc atagatttag
    21001 ccagtaagta tgattttata ttctcagcct cctctttaaa attatatttt tcaaaataac
    21061 ttgaaaaatc tggagtattt aaaagaatgt cacttgtaac ttgtttcaat tgctttattt
    21121 tttggatttt ttctccctgg ctcatcatta ataaatcgta tagccagtat tcttgttgtt
    21181 tagttatttt attgtctaat ctagctattt ttattagatg acatattttt ataaaggccg
    21241 ataatcctgt tttttgatca ccttttgaaa aggataaaaa atcacctgtt aataaagaaa
    21301 atccaattag tgctaaacta aaataatctt gttgaactga gctgatagtt aggtcagtat
    21361 ctggattaac catcattggt gttttaactc gtcttacctt atccttgata ggagtcattg
    21421 tctccaagtc tattaatgtt atatcacctt ttttggtata tataaaattt gtaggagcta
    21481 tatctacaat tttatattcc cagtttatgg atgtaactta ctatattcac aatgttatcc
    21541 agtgtttttt ctctaatatt taaggagtgt tctgtttctc gaataaattc ttcaaagttt
    21601 aacccgtcaa cttgttcctg aacaagaaaa taatcatcca cgatataaaa ttcatcagtt
    21661 aaattagtag tataactttt atcggctaat ttttttagca tgtgagcttc attttttata
    21721 tcatcaagag ctgtccattc tccttcagca tcataattca caaaaggtct tgactgcttg
    21781 atgattactt tttgcccgtc cgattttcta attgcccgat aaacatttcc tttatttgat
    21841 ctcttaataa ttttttccat tttgtattta tttattgcag agtccttact tgaaacttca
    21901 catgtggttt gaaaataaat ccttttttct tcttctgaaa ataaatccat tttccagcta
    21961 ggaagactag ggaaattttg tctcttatct tctacatagt tcttcctttt ttcatctagc
    22021 aataaataga tgactttttt atttttttca tcataagctt gtttttttaa aaaagcccca
    22081 tatctataat gaactggaga atgcattcca cattgatagt cagatagtat ttttggagcc
    22141 ttaaattctg acagtctatt cgtaagatta caaatcatac tcttagcttt agattctgac
    22201 ttaggatata gagttataaa tttgttagca gtagggctca tttccctagg ggaattaatt
    22261 ttttttaatt cctctaaatt tttaacaact ttaaagctac aattatttag ttgggatagt
    22321 ttatacacaa tcttaaaaat atttacagcg tcttttattt gggagcttat gtgtattttc
    22381 cacccttgtg caggcatctt atctgataaa taatagtaat tccaaatatc accctgttgg
    22441 aaatttttat cacattttaa acattctttc tctaataaat ccataacaaa ctcctaatta
    22501 ttaaaatagg ctagagacac agcactagcc tattccgtaa gtaaagctac ttattttata
    22561 taaaatataa ctagcgacct gtaattaaac aagtaaaaga acaatactta gggagacctg
    22621 tgacaatgat atcttcttct ggaagtagtt ctaattcaaa caatgtttgt tccatgactg
    22681 tatcctcctg ttatgataaa ttccagaaag catagagcaa attcgtggtt cgtctgacta
    22741 aatttcttat ctgaacacca ccttcaaata caaattttag tcaaactttt attttttcta
    22801 actttctaat tatattctac catgtgtttt tatatgtcaa gaattttctg aataaactgt
    22861 ttgtttttta ttttttgaca tttttgcaaa ttgtttaaaa tctttcaatt ggattcactt
    22921 attactttag gacattgaaa cttctttcgc cagaggtgct tatattactg ggaagttgaa
    22981 gattgaaatg tggtataata agaggtaata gagttttgga aagtgagaga agatgatttc
    23041 aaagagatta gaattggtag cttcctttgt gtcacagggg gctattttac tagatgtggg
    23101 aagtgaccat gcttatctgc ctatcgagtt ggttgagaga ggccaaatca aaagcgctat
    23161 tgcaggtgag gtggtggaag gtccctatca gtctgcggtt aaaaatgttg aggctcacgg
    23221 cctaaaggag aaaatccaag tccgtttagc caatggcttg gcagcttttg aagagactga
    23281 ccaagtgtct gtcattacca ttgctggcat gggtggtcgt ttgattgcta ggattttaga
    23341 agaaggtttg gggaagttag ctaatgtaga gcgtttgatc ctccagccca ataatcgtga
    23401 agacgacttg cgtatctggc tacaggatca tggattccag attgtagcag aaagcatctt
    23461 agaagaagct ggaaagtttt atgagatttt ggtggtggaa gcaggacaaa tgaagctatc
    23521 agccagtgat gttcgctttg gaccgttctt gtgcaaagag gtcagtccag tctttgtcca
    23581 aaaatggcaa aaagaagctg ttaagctaga gtttgccctc ggacaaatcc cagacaaaaa
    23641 tctggaagaa cgtcaagttc tagtagataa gattcaagcc atcaaggagg tgctccatgt
    23701 tagcaagtga agtgatccaa gcttatgaag ccttttgccc tcaggaattt tctatggagg
    23761 gagacagccg tggtctgcaa attggcactt tagacaaggg tatccaaagg gtcatggttg
    23821 ccctcgatat tcgtgaagag acagtggcag aggccattga aaagggtgtg gacttgatta
    23881 tcgtcaagca cgcgcctatc tttcgtccta tcaaggactt gcttgccagc cgtccgcaaa
    23941 atcagattta catcgacctg attaagcatg acatcgcagt ttatgtcagc cataccaata
    24001 ttgatatcgt tgaaaatggg ctcaatgact ggttttgtca gatgctagaa atcgaggaga
    24061 cgacttatct gcaggaaaca ggtccagaac gtggaattgg acgtattggg aatattcagc
    24121 ctcagacctt tggagaattg gcccaacatg tcaagcaagt ctttgaccta gatagccttc
    24181 gaatggtgca ttatcaagag aatgatttgc agaagcctat ttcaagagta gcaatttgtg
    24241 gtggaagcgg gcagtctttc tataaggatg ctttggcaaa gggggcagat gtctatatca
    24301 ctggtgatat ctactaccat actgctcagg atatgctgtc tgatggcttg ttagcattgg
    24361 atccaggtca ctatatcgaa gtgctttttg tggaaaaaat cgcagcactc cttactcaat
    24421 ggaaagagaa aaaaggctgg gaactagaga ttttacctag tcaagcatcg accaatcctt
    24481 tccgtcatat ctagttagaa agtgaagaca atgaaaaaag ttgccattat tggagcagga
    24541 attgtgggag caacagctgc ctactacctc tcgagagaaa gtgacctaga ggtgaccgtt
    24601 tttgaccatg agcaaggtca agccaccaag gccgcagcag gaattatcag tccttggttt
    24661 tccaaacgcc gtaataaagc ctggtacaag atggcgcgct tgggggctga tttttatgtg
    24721 gatttattag ctgatttaga gaaatcagga caagaaatcg acttttacca gcgttcggga
    24781 gtctttctct tgaaaaagga tgaatccaat ttggaagaac tttatcaact ggccctccag
    24841 cgcagagaag aatctccctt gatagggcaa ttagccattc tgaaccaagc ctcagctaat
    24901 gaattattcc ctggtttgca gggatttgac cgcctgctct atgcttctgg tggagcgaga
    24961 gtagatggcc aactcttggt aactcgtctg ctagaagcca gtcatgtcaa gctggtcaaa
    25021 gaaaaagtga ctctgacacc gttagcatca ggctaccaga ttggtgaaga ggagtttgag
    25081 caggttattt tggcgacggg agcttggttg ggggacatgt tagaaccctt gggttatgaa
    25141 gtagatgttc gtcctcaaaa aggacaacta cgagattatc agctggtcca agatatggaa
    25201 gcttaccctg ttgtcatgcc agaaggggag tgggatttga ttccttttgc aggtgggaaa
    25261 ttgtccttag gcgctaccca cgaaaatgac atgggatttg atttgacggt agatgaaacc
    25321 ttgctccaac aaatggagga ggccaccttg actcactatc tgattttggc tgaagctact
    25381 tcaaaatctg agcgtgttgg aatccgtgcc tacaccagtg atttctctcc tttctttggg
    25441 caggtgcctg acttaactgg tgtctatgca gccagtggat taggttcatc aggcctcaca
    25501 actggtccta ttattggtta ccatttagct caactgatcc aagacaagga gttgaccttg
    25561 gaccctgaaa actacccaat tgaaaactat gtcaaacgag taaaaagcga ataatactca
    25621 atgaaaatca aagaacaaac taggtaacta gccgcgggtt actcaaagca ctgctttgag
    25681 gttgtggata gaactgacga agtcagctca aaacactgtt ttgaggttgt agatagaact
    25741 gacgaagtca gtaaccatac ccatggcaag gcgacgttga cgtggtttga attggatttt
    25801 cgaagagtat aagcatttta ctgaaatttt atcaggtagt ttaggatggc aaatgacatt
    25861 ccctgtcaaa aatggtaaaa taagaaaaaa taatctgaga atcgaggaaa aaagatgcaa
    25921 gaaaagattt tggtaactgg tggtgccggt tttattggaa cccacactgt tattgaattg
    25981 atccaagcag gccatcaagt tgttgtggtg gataatcttg tcaatagcaa tcgtaagagt
    26041 ttagaagttg ttgaaagaat tacaggagtt gagattcctt tctatgaggc agatattcgt
    26101 gacacagcta ccctcagaga tattttcaag caggaagaac cgactggtgt cattcacttt
    26161 gctggtttga aggctgttgg cgaatcaaca cgtatccctc ttgcctacta tgacaacaat
    26221 atcgctggaa ctgtcagcct tttgaaagcc atggaagaaa acaactgtaa aaacatcatc
    26281 ttcagttctt ctgcgacagt ttacggggat ccgcacacag tgcccatctt ggaagatttc
    26341 ccactttcag tgaccaaccc atacggtcgt accaagctca tgctagagga aattttgacc
    26401 gatatttaca aagcagactc agaatggaat gttgtcttgc ttcgttactt taacccaatc
    26461 ggagcccatg agagtggtga tttgggagaa aatccaaacg gtattccaaa caatctcttg
    26521 ccatatgtga ctcaagtagc cgttggaaaa ttagagcaag tgcaagtgtt tggagacgat
    26581 tacgatacgg aagatggaac aggtgttcgt gactatatcc acgttgtcga tttggctaag
    26641 ggtcacgttg cagctttgaa aaaaatccaa aaaggttcag gactaaacgt ttataacctt
    26701 ggaactggta aaggttactc agttcttgaa attatccaaa acatggaaaa agcggtggga
    26761 cgtcctattc cttaccgcat cgtagaacgt cgcccaggtg atatcgctgc ctgctactca
    26821 gacccagcaa aagctaaagc agaactcggt tgggaagcag aactcgacat cacccaaatg
    26881 tgtgaagacg catggcgttg gcaaagcaag catccaaatg gatttgaaga ctaagatgat
    26941 gatttcaatc atcgtccctt gtttaaacga agaggaagta cttcctcttt tttatcaggc
    27001 tctggaagct ttacttccag atttggaaac agaaatcgag tatgtctttg tcgatgatgg
    27061 atcaagtgat gggaccttgg aactcttaaa ggcctatcgg gagcaaaatc cggcagtcca
    27121 ttatatttct ttctctcgaa attttggcaa agaagcagcc ctttatgcag gcttgcaata
    27181 tgcgacagga gatttggtgg tggtgatgga tgcagacctc caagatcctc ctagtatgtt
    27241 gtttgagatg aaaaatgtac tagacaaaaa tgtagacttg gactgcgttg ggacacggag
    27301 aactagtcgg gagggagaac ccttctttcg cagtttctgt gctgttctct tttatcgcct
    27361 catgcaaaaa atcagcccag tagctctgcc gtcgggtgtc cgtgattttc gtatgatgag
    27421 aaggtctgta gtcgatgcca ttttaagctt gaccgagtcc aatcgttttt caaaggggct
    27481 cttcgcctgg gtgggtttca aaacctacta tttggactat ccaaatgtcg aaaggcaggc
    27541 tggcaagaca aattggagtt ttaggcaact ctttttctac tccattgaag ggattattaa
    27601 tttttcagat tttcccttga ttatagcctt tgtagctggt ctcctatctt gttttctttc
    27661 tctgctgatg accttttttg ttgtggttcg gaccctcatt ttgggcaatc cgacatctgg
    27721 ttggacctct ctgatggctg ttattctctt tcttggaggt attcaactct tgaccattgg
    27781 gattctcggc aagtatatca gtaagattta tttagaaacc aaaaaaagac cgctttatct
    27841 tatcaaagaa aaaagttatc atagatcatg aaaaagtagg agttatctag cgcagtcact
    27901 tttttgtaat tataattctt ttggtctgga acttgagtga tttttaaaaa aattagggat
    27961 ttgacatgat ataaatgaac tatgtggaat aaatttactg caaagacgat aacttaataa
    28021 ataaaaaatt caggagggtt attttatgct aagtaaagag gagtatattg aagaaatcgg
    28081 gcttatagag aaacaaaatt atgtagaggt tgaactgtat cctttagttg ctgatataat
    28141 taatccgact ttaaaaaata gtttatcaaa aagatatgtc ttcggaagaa gaaagagtaa
    28201 tatggggcaa atatattatg gtctaagtaa ttttcctgat atagttattt tggataagaa
    28261 ttatcaaaat aaagccagaa aaagtataga aattgaagaa tggaagaaac ttcgaggatg
    28321 cgtggaaatt aagagtttaa agcatgattt aattacagag gaaaaaatca aatcaaccat
    28381 atcgaacagt tttgaacata taactggaga gatgggacaa cttattggag accttttatg
    28441 gtataaaaaa gtaatttata caaatggaat tgaatggaga tttctaagtt tagatgacaa
    28501 ggaagagata gataatacaa ttgttcaagt agttaacaaa agaatagaaa cagaagaagc
    28561 gggaaattct tttgattggt ggaagaatat taaagattta tcgttcaatt atacggatat
    28621 atatttatct aaagattgta tataagagtg ggatgaattt gtaaagaaag taaaggaaat
    28681 agaatggtag gagtctattc tcattgagaa atttagatta taagtcgcag taagatttat
    28741 ttagagacta aaaaaagacc actttatctt atcaaagaaa aaagtgacct tcctgatttt
    28801 acagaaaaaa ataaagtgaa aagactataa ttttacatgg aaatgtgcta aactagaggg
    28861 agtggattat cgccattgat ttaggcttcc tggtttccaa tcagggcgcg agttgggcaa
    28921 tttttaacag cctctaagat atcctgacta ggagaaattt ctttttccag ttggtcaggg
    28981 tcatcgtaaa aacgcacgat tccattatcg tggtaatcaa ataaatcaga ataagtttgg
    29041 caaagcccac aggcgataca tcgttcaggt ataagtgtga ttttcatgtt catatttata
    29101 ttgtaataag aaaagtaaaa aaaaacaagg agtaaggtat ggcaaaagaa ccgtggcaag
    29161 aagatatcta tgatcaagaa gaatcaagag cagagcgtcg gcatcgaaac cacggagggg
    29221 ctgataggat ggctaatcgt attttgacga tcctagctag tattttcttt gtaattgtgg
    29281 tggtgatggt catcgttctc atctatctat catcgggggg gagtaatcgc acagcagcct
    29341 taaaaggctt tcatgattct gatgccagtg tagtacaaat ctcatcttca agtagttctc
    29401 agcctgagca gagttcagag ccagaatcta cttctagtag ttcagaagaa gctgctaatc
    29461 ctgaaggaac gattaaagtt ctcgcaggag aaggggaagc agctattgcc gctcgtgcag
    29521 gaatctccat tgctcagtta gaggccttga atcctgggca catggctaca ggatcttggt
    29581 ttgctaatcc aggtgatgtt ataaaaataa aataggagcc gatatgaaaa caattcaaat
    29641 tgctattgat ggtcctgctt ccagcggtaa gagtacggtc gcaaagatta ttgctaagga
    29701 ttttggattc acctaccttg atacaggagc tatgtatcgt gcagcgacct atatggctct
    29761 taagaaccaa ttaggagttg aagaagtcga agcccttcta gccttgttgg accagcatcc
    29821 aatcagcttt ggacgttcag aaactggaga ccagcttgtt tttgtaggag atgtggatat
    29881 tacccatcct atccgtgaaa atgaagtgac caatcatgtt tctgctattg cagcaattcc
    29941 tcaagtgcgt gagaaactgg tttctctcca acaagaaatt gcccagcaag gcgggattgt
    30001 catggatggt cgcgatattg gaactgttgt attgccacaa gcagaattga aaattttcct
    30061 agtagcttct gttgatgaga gagcagagcg tcgttacaag gaaaatattg ccaagggaat
    30121 tgaaacagac cttgaaaccc taaaaaagga aattgctgcg cgtgactaca aggatagtca
    30181 tcgtgagact tctcctctca aacaagcaga ggatgctgtc taccttgata caactggttt
    30241 gaacattcaa gaagtagttg aaaaaatcaa agcagaagct gaaaaaagaa tgtaaatgta
    30301 gaaaagccga tagattgata tcggtttttt tctaaatttg tcaaaatgct gattttaggg
    30361 tataatagtt gctgttcgag aaattttgat tttcaaagaa tagtgaatga tgataaggag
    30421 aaaccatgaa caatttacca aattgtccaa aatgtaactc agagtatgtc tacgaagacg
    30481 gtgccctact ggtttgccca gagtgtgctc atgagtggaa tcctgctgaa gttgcagaag
    30541 tagaagaagg tcttgtcgct atcgatgcca acggaaataa attggctgat ggtgatacag
    30601 taactctcat caaggacttg aaagtaaaag gtgcgccaaa agatttgaaa caagggacgc
    30661 gcgtgaaaaa tatccgcatc gtagaaggcg accacaatat cgactgtaag attgatggtt
    30721 ttggtgccat gaaacttaaa tcagagtttg tgaggaagat ttaagcatgt caaaacacta
    30781 taaacttgta ttttatagcc gtatcttctt gtttctagcg gcttttacgg gagtttatct
    30841 tgaaattact aagcatggtg gttttgggat gcttctctat tacacggttc tgtccaacct
    30901 cttggtaact atttttaccc tttatcttct aaaggttatg agccgtgtag gtgaaaattg
    30961 gcaaaggcca agtctcttgc gcttaaaagg tggggtcacc atgagtatca tgattacctg
    31021 tgtgatttac catttcctct tggcgcccat tgcgactaat ttctataccc tagaaaattt
    31081 cctttgccac tatatcgttc ccatctggtt tttagcggat accctctttt ttgacaaaca
    31141 gggtcaatac aagatttggg atccagcagt gtggaccatt ttaccctttc tgtatatgat
    31201 gtttgctctt tttaatggct tggttctaaa actcaatatt ccaaatgcca aggataatcc
    31261 tttcccttac ttctttttga atgtgaacaa gggttggaat gttgtgttta agtggtgtct
    31321 gattatcttt gttgcctata tggtagcagg atttattttc tactttatca agcaaatcaa
    31381 gagaaagtca tcctaatact cttcgaaaat ctcttcaaac cacgtcagtt tccatctgca
    31441 acctcaaaac actgttttga acaacctgcg gctagcttcc tagtttgctc tttgattttc
    31501 attgagtata agatacaagg gattcatttt cgaatttaag aaggagtcta caaaccaatg
    31561 aggcttcttc ttttcttgct tatttgataa ctatcaaaaa agtagaagat taagagaaat
    31621 atagaaagag atttcatgaa acaattttta gaacgggcca gcattttggc tctctccctc
    31681 gttttgatta cctccttttc catttcgagt gccctaccag ccatgtttga ctattatcag
    31741 ggttattcta aggaacaaat tgagctcttg gtgagcttgc cttcctttgg aatcatgatg
    31801 atgttactgc taaatggttt cttagaaaaa atatttcctg agcgcttaca gattagtttg
    31861 ggcttgctga ttttatcatt gagcggtaca gctcccttct ggtaccaagc ctatcccttt
    31921 gtctttggaa cacggcttct ctttggtttg ggtcttggga tgatcaatgc caaggccatt
    31981 tctattatca gtgaacgcta ccaaggaaaa aggcgaattc agatgttagg gctacgcgct
    32041 tctgcagagg tcgttggagc ttctctcatt accttggccg tcggtcagtt gttggccttt
    32101 ggttggacag ctatctttct agcctatagt gctggatttt tggtgctgcc cctttatctg
    32161 ctctttgtcc cttatggaaa atcaaagaaa gaagtcaaga aaagagcgaa ggaagcaagt
    32221 cgtttaactc gagaaatgaa aggcttgatt tttaccttag ctatcgaagc ggcagttgta
    32281 gtttgtacca atacagctat taccatccgt attccaagtt tgatggtgga aagaggattg
    32341 ggggatgccc agttatctag ttttgttctt agtatcatgc agttgatcgg gattgtggct
    32401 ggggtgagtt tttctttctt gatttctatc tttaaagaga aactgctcct ctggtctggt
    32461 attacctttg gcttggggca aatcgtgatt gccttgtctt catccttgtg ggtggtagta
    32521 gcaggaagtg ttctggctgg atttgcctat agtgtagtct tgacgacggt ctttcaactt
    32581 gtctctgaac gaattccagc taaactcctc aatcaagcaa cttcatttgc tgtattaggc
    32641 tgtagtttcg gagcctttac gaccccattc gttctaggtg caattggctt actaactcac
    32701 aatgggatgt tggtctttag tatcttagga ggttggttga ttgtaatctc tatctttgtc
    32761 atgtacctac ttcagaagag agcttaggat tgattcctaa gtttttcttt tgatactcaa
    32821 tgaaaatcaa agagcaaact atagttgatt gagtttggaa tagtacgctg tagctgctaa
    32881 aatatttcta gaaattaatt tgactttcct aatagagttg ttcatatctt atttcaatct
    32941 actatagaaa gctagccgca ggttgctcaa aatagtgtgt tgaggttgca gatggaaact
    33001 gacgtggttt gaagagattt tcgaagagta tgagattttt atacccatac aatatttgtt
    33061 ttaggtcctt gtttacagac taatttcttg ataaaataga ggctatgacg agatataaag
    33121 caactatttc ctatgatggt tatgcctttg ctggctttca gcgccagcct catgcgcgta
    33181 gcgttcagga agaaattgaa aaaaccttga ccagattaaa taaagggcaa gccattactg
    33241 ttcacggtgc tggtaggaca gatagtgggg ttcatgccct gggacaggtg attcattttg
    33301 acctgcctta tcagatggat gaggagaaac tccgttttgc cttggatacc cagtctcctg
    33361 aagatattga tgtgatttcg attgagcttg tggcagatga ttttcattgc cgttatgcca
    33421 agcatagcaa gacctatgag tttactgtgg atagaggacg tcccaaaaat ccgatgcgcc
    33481 gtcactatgc cacccacttt ccctacccac tcgatgtgga acgaatgcag attgcaatca
    33541 aaaagctaga gggaacccat gattttaccg gttttacagc ctctgggact agtgtagagg
    33601 ataaggttcg caccatcaca gaagctagtt taatagtcga tgagacagga caatttttga
    33661 cctttacctt ttcaggaaat ggtttcttgt ataaacagat tcgcaatatg gtggggactc
    33721 ttctcaaaat cggtaacaac cgcatgccag tagagcagat tgatctcatc ttggagaaga
    33781 aggacaggca acttgcaggt cccactgcag caccaaatgg tttgtattta aaggagattc
    33841 gttatgaaga ataatcgtat tttagcactt tctggaaatg atatttttag tggtggtgga
    33901 ctgtcagctg atttggctac ctataccttg aacggcttgc atgggtttgt agcagtgact
    33961 tgtttgacag ccttgacaga aaaaggattt gaagtctttc caactgatga taccattttt
    34021 caacatgaat tagatagctt acgtgatgtg gaatttgggg gaattaagat tggtcttctc
    34081 cctactgtca gtgtggctga gaaggccttg gactttatca aacaacgccc aggagtacct
    34141 gtggtgttgg atcctgtctt ggtctgcaag gaaacgcatg atgtagctgt cagtgagctc
    34201 tgccaagagt tgattcgctt tttcccttat gtcagtgtga ttacgcctaa tctcccagaa
    34261 gcagaattat tatccggtca ggaaattaaa accttggaag acatgaaaac tgcagcgcag
    34321 aaattgcatg atttaggagc gccagcagtc attatcaagg gaggcaatcg tcttagtcag
    34381 gacaaggctg tggatgtctt ttatgatgga cagaccttta ctatcctaga aaatccagtt
    34441 atccaaggcc aaaatgctgg tgcaggttgt acctttgcct ctagcattgc cagtcacttg
    34501 attaaaggtg ataaactttt gccagcagta gaaagctcta aggctttcgt ttatcgtgct
    34561 attgcacaag cagatcagta tggagtaaga caatatgaag caaacaaaaa caactaaaat
    34621 cgcccttgta tccctattaa ccgccctttc tgtggttcta ggttatttct taaaaatccc
    34681 aacacctaca ggaattctaa ctcttttaga tgctggtgtc ttctttgcgg ccttttactt
    34741 tggtagtcgt gaaggagcgg tagtcggagg actagcaagt ttcttgattg acctcttatc
    34801 aggctaccct cagtggatgt tctttagctt ggtcaaccat ggcttgcagg gatttttcgc
    34861 aggatttaaa ggaaaaagtc agtggttagg ccttatttta gcaactattg ccatggtagg
    34921 aggctacgcc ttgggttcta ctttgatgaa tggctgggca gcagccctcc cagaaattct
    34981 accaaatttc atgcaaaata tggtagggat gattgtagga tttattctta gtcaaagtat
    35041 caagaagatt aagtaatact cttcgaaaat ctcttcaaac cacgtcagct tccatctgca
    35101 acctcaaaac actgttttga gcagcctgag gctagctttc tagtttgcac tttgattttc
    35161 attgagtata aagaggttga gtaaaaagta tttcaaataa gaaaaacgca tagtatcagg
    35221 tgttgaatga actgcccccc aaaagttaga cagaaaaaat ctaacttttg gggtgttttt
    35281 attatgaaat taagttatga tgataaagtt cagatctatg aacttagaaa acaaggatat
    35341 agcttagaga agctttcaaa taaatttggg ataaacaatt ctaatcttag gtatatgatt
    35401 aaattgattg atcgttacgg aatagagttc gtcaaaaaag gaaaaaatcg ttactattct
    35461 cctgatttaa aacaagaaat gattcataaa gtctggcatg aaggctggac taaagataga
    35521 gtttctcttg aatatggtct cccaagtcgt acgatacttc ttaactggct agcacaatac
    35581 agaaaaaaac gggtatacta ttgttgagaa aacaagaggg agagtaccta aaatgggacg
    35641 taagccacaa aagagacctg aagagaggac agaattagaa cgtcttcaag cagaaaatga
    35701 gtacctgaga gcggagaatg ccatcctaaa aaagttaaga gaactccgat tgaaggagga
    35761 aaaagagaaa gaagaaagac agaaattgtt caagaattaa tgactgagtt tttgttagtt
    35821 cttcttctaa aatccattaa actagctcgt tggacctact actatcactt gaaacagcta
    35881 gacaaaacag ataaggacca agagcttaaa gctgaaattc aatccatctt tatcgaacac
    35941 aaaggaaatt atgcttatcg ccgggttcat ttagaactaa gaaatcgtgc ttatctggta
    36001 aatcataaaa gagttcaagg cttgataaaa gtactcaatt tacaagctaa aatgcgacag
    36061 aaacgaaaat attcttctca taaaggagac gttggcaaga aggcagagaa tctcattcaa
    36121 ggccaatttg aaggctctaa aacaatggaa aagtgctaca cagatgtgac agaatttgcc
    36181 attccagcaa gtactcaaaa gctttactta ccaccagttt tagatggctt taacagtgaa
    36241 attattgctt ttaatctttc ttgttcgcct aatttagaac aagtacaaac aatgttggaa
    36301 caagccttca cagagaagca ctacgagaat acgattctcc atagtgacca aggctggcaa
    36361 tatcaacacg attcttatca tcagtttctt gagggtaagg gaattcaagc atccatgtca
    36421 cgcaagggca acagccaaga caacggtatg atggagttct tctttgggat tctgaaatcg
    36481 taaatgtttt atggctatga gaaaacattt aaatcactta atcaattgga acaagctatt
    36541 gtagactata ttgattacta caacaataaa cgaattaagg taaaactaaa aggacttagc
    36601 cttgtgcaat acagaactaa atccttcgga taaattaatt gtctaacttt tggggggcag
    36661 tacatattca acacctgata ctatgcgttt tattgtggga agattttcct cattttctag
    36721 tgaaattgaa ctttttctaa catcttttga tagatgactt cttaatcaat tatcatttcc
    36781 attgttgttt cttggggttt catcccttgt ctttgataaa agcggagagc accctcgtta
    36841 tcattccaga cacttagggt tagattattg cagccaattt ctttagcata atagcagaaa
    36901 aatgacaata aaatagcccc tagatatttt gaaaggaaag cataggggct atatagttta
    36961 atcaaaataa agcaaatctt tgctggtgct tgtaaagagg tcgaagccat ccttggtaac
    37021 aacaccgcag tcttcaatac gaacaccgac tttaccaggg atatagatac ctggttcaac
    37081 agagaagcac atgccttctt cgatgaccat gtcgtttcct tccatgatag atgggaattc
    37141 atggacatcc ataccgatac catgcccgag acggtggttg aagtactcac cataaccagc
    37201 tttttcgatg acctcacggg cagcgcggtc cacttcatga gcagtcacac ctggcttgat
    37261 aaagtcaaga gcagcttgtt gggcttcaag agtcaagttg taaatatctt tcttgaattg
    37321 gtctggtttg ccgacagcga ctgtacgagt catatctgac gcatagccat tgaccagaac
    37381 acccaggtca aagaggagaa gagcatcatt ttcaacctta ttagctgctg gaatgccgtg
    37441 tggattcgca gcattatcac cagtcaagac catggtatca aagctcattt cgtaaccttc
    37501 acgtttcatg gcaaagtcaa tttgtgcgat gatatctgtc tcagtcttat caagagaaat
    37561 attgtcaaaa ccaacatgaa cagccttgtc agcataaaga cctgcaacca tcattttttg
    37621 cacttcatca gctgatttga tgaggcgcat gcgttggata cgaggagtga ggttgtcaaa
    37681 ctcagcagtc tcaaaaactg ttttcaaacc atggtatttg gtcaagatga gattgtcaaa
    37741 ctcaacagcg acacgtttga agtcaagttg tggaagagca tgtttgattt tttgccatgg
    37801 attttcagaa tcgacatagc ccactactgg gaaggaaacg gtgctacttg cacgttctac
    37861 ttcaagagct gggacaaaga ggagaggttc ctgatccgct aggacaaaga ggaacatttg
    37921 gcgttcatgg ggatcactgt aaaaaccagt gaggtaattg attgtgacgg ggtcagatac
    37981 gacagcgacg tctagttttt ctgattcaag atatgttagg atttgttgta atttagacat
    38041 atgctacctt ctttctaccc ctctattttg gcaaaaaatg agagaaaatg caagggagaa
    38101 gggtaaaaga aaagacaaaa aactccgaca agataacgga gttctttgat atcattttcc
    38161 tttgtttaag aaaagtcagc cttgcttttg acgtcactgt actcttcagc gatttcttgg
    38221 ctgaggatat cctcataggc agggagttgg atatcctgac catatttctt cttgagggca
    38281 gtagtaacta ggcgataagc cagattggca ttacgagaga ggataggccc gtggaagtag
    38341 gaaccaaaga cattcttata atgaacccct tcaccgacct tttcttcgtt gtttccattt
    38401 ccatagacaa cctgtcccag cggtttttgg tcatcagaga ggaaggtacg gccttggtga
    38461 ttttcaaatc catagtaggt ttcatcgaaa tcttcattgt gaatcttgat gtcaccgata
    38521 aaacggttat tggtctggtt gagcgtgtag tgtcccatga cccctagccc ttcgatacgt
    38581 tttcctgaag cttcaacata atattgaccc aatagttgga aaccaccgca gatagccaga
    38641 actacaccgt cgttttggat gtagttgtca atgctctctt ttttagcagg taggtcgtct
    38701 gcaatgatac tttgttcaaa gtcttgacca ccaccgaaaa aggcgatgtc gtagtgattt
    38761 tcatcaaagt catcatggag agaaacgatg tcaacggtca catgggctcc cagtttttca
    38821 gccacatact tgagcatgag gatgtttcca ttgtccccgt aggtattcat gagatttccg
    38881 tagaggtggg caatgttgag ctgatagggg taattgccat cttttgagga aagtgaagta
    38941 taaaccatta gttcatctcc tttctaacaa tctgacgact agccagcagt tcacgaaatt
    39001 ccagcatggc agtataagtt gccagaatat aggcatgctt gcagtcttga ttctcaatgg
    39061 tcttgagaac ttgctccaga ttactcgttt cagtgatttt ctcagctgga tagccagtca
    39121 ctcggaggcg acgagcgatt tcagaatgac gaacaccgcc agcgttgatt tcaggaatgt
    39181 ccatgtcagt gatttgttca aagtctgcat cccagatcca gctagtgtca attccatctg
    39241 catagttggc attaaggagg acagataggc taaatggata aggtgctagt ttgatcattt
    39301 cgatagcttg ggttgcaccg actggatttt taatcaagac aagggtacat tccttgtcac
    39361 cgatatgaaa ggtttcttgg cgtccaaaga cagcacggct cttgtcaaat ccctgtttga
    39421 tgagttggga atctgcgccg aggaaacggg cgatggccac agcagctagg gcgttataga
    39481 tattatagag cccgccgatt tggataccgt attcttggcc gtctatgaca aagcgagagc
    39541 gattgttggt caactcaacc agttttgtca aacgatagtc gagatcagga cgtttacatc
    39601 cacaaccttc acagatatag gcacccaagt ttgcataggt attatgctca tatttgagga
    39661 tgccttggca gtcaggacag aggattcctt cggtattata gtgagccagc tgggctggac
    39721 ctttctccaa gtcaaaacca aaatactcta caggatttgg aatagctggc ttgtagaaaa
    39781 gtggactgtc tccattaagg agaacagtgg cagtaggcac cttacgaatg gcatccaaaa
    39841 tcatcttgta agttgtgtaa atctcaccat agcggtccat ctggtcacgg aaaatattag
    39901 taataacaaa taagcttggc tggatatagt cacagatacg agatagactg gcctcgtcga
    39961 tttctaggac ggcaatattt ttcccagttt tagaagattt ggctgttaag aaggttgtcg
    40021 caattcctgt aatcatgttg gcaccgcttg ggttggtcag aacttgacca taaacttctt
    40081 ttaaaatacc gacagtgagg gcagttgtca gggtttttcc atttgttcca gtgacaacga
    40141 caatctcgta gttcttagct aggttttgta aaatatcttt atcaaattga agggcgactt
    40201 tacctgggag cgtacttcca cgtccaagac ggcttaaaac gaagtgggaa gaacgcccag
    40261 caagaaggcc caaagtagtt tttaagttca tgtttctatt atatcacaaa agagggaaaa
    40321 gacagatttg gtgtttcgta gaagtaaaaa cagcccaaag agggctgtca gttaagatga
    40381 gatttcaact taaatcgcaa acaagtcatt caagttctca gccaaggttg ggtgagtgaa
    40441 aatttgtttt gtgaagtaag tgtaaggaat cttgttgtcc atagcaacag taatgatgtt
    40501 gatgatttct tgagaacctt ctgagaagat gcttgcacca agaatttctt ttgtttcagt
    40561 attaacaaca gctttgaaag ctccgcgaag gtctccattt acgtgaccac gaggcatggc
    40621 tgcaacaggg atttctttca ctgcgtatgg aagtttcaaa tcagctgctt ggctttcagt
    40681 caaaccaact tgtgaaagtg caggtgtgat gaacatagta tttggcacat tgagacggtc
    40741 ctcaagtgtg tagctgccat ctccagcaag gtagctgtaa acaacacgga agtcatcaag
    40801 tgaaatgtaa gtaaattgaa gaccaccgtt gacatctcca actgcaaaga caccaggaac
    40861 gtttgtttga cagtgtttat ctactttaat agcgccacgt tcagttagtt caatatctgt
    40921 attttcaagt tgaagtggtt ctacatttgg tttgcgtcca gtcgcgtaga gaagggcgtc
    40981 gaaacggtaa gtttcgtctt cagttacgac aagcacttgg tcaccgtcgt ttttaatttc
    41041 agtagtatgg atattttgaa gcaattcaat accgtcttct tccatgtatt gtttagcaag
    41101 agctgcgatg gaaggttctg cacgaggtag gaatgtatcc aaggcatcta ggactgtaac
    41161 cttgcttcct agtttattgt aaaggccagc aaattcaaga ccgatatttc cgccaccaag
    41221 gactccaagt ttttcaggca atttatccaa gctttggata cctgttgagt caaagacgtt
    41281 tttgcttgta gcaagtccag ggattggcaa gacgtttgaa acagcaccag tgttgatgac
    41341 gatagtttca gcagtcaatt cttgtttttc atcaccagct tggatttcga tgactttatt
    41401 tgaaaggaag tgagcttcag catcaaagat attgacgcca gtaccagcaa cagtcgtata
    41461 gtttttaccg ttgaggcgac cagtgatcgt gtttttggta gcaatgactt cttcaaaaga
    41521 caagtctttc tcagcagcaa ccagcaaagt tttagttggg atacaaccga tgttgataca
    41581 agttccaccg tacatagctt tgctacgttc aacgagggca acttttttgc cagctgaagc
    41641 caatttacct gctagtgttt taccagcttt accaaatccg ataacgatta aatcataagt
    41701 taacatttag aattcctcct attcgaatta cattctagca caagaaaaaa acttttgcac
    41761 aattctgcta cgaaaaagac agaaaaatat gaaaacgctt ttcaggcaaa aaactagtaa
    41821 ttttttgaca agcttgataa actaaaatcc tatctatttt tatataagat agaataaatg
    41881 ttcgaaaata ggaggtttta tgaaatcgaa tcgttatatt attgcctttg ctggggtcat
    41941 tttacactta atgctgggtt cgacttatgc ctggagtgtt tatcgtaacc ctattattga
    42001 aaaaacggga tgggatcagg cttctgttgc cttcgccttt agtctagcaa tcttttgttt
    42061 gggcttatcg gctgcattta tggggcgttt ggtagaaaaa tttggtccga gagtcatggg
    42121 gagtctatct gcttttctat acgcaggtgg aaatatctta acaggatttg caatagaccg
    42181 tcaggaactg tggttgttgt atctagctta tggcatttta ggtgggcttg gtttgggagc
    42241 aggctatatt acccctgtgt caacgattat aaaatggttt cctgataaac gtggtctcgc
    42301 aacaggttta gcgattatgg ggtttggttt tgcttcttta ttgactagtc ccatagcgca
    42361 acatctcatc gcaggggtag ggcttgtaga aactttttat attttaggtg caagttactt
    42421 tattatcatg ctcctagctt cacaattcat taagcgtcca aatgagcaag agcttgcaat
    42481 tttatctgct tcagggaaag aaaaaacaga ctctctgacg caagggatga ctgcaaatca
    42541 ggctctaaaa agcaatcggt tttatatact ttggattatt ttctttatca acatagcttg
    42601 tggtttaggc ttaatttcag cggcatcgcc aatggcacag gagatggctg gcttgtctac
    42661 aagtcatgca gcagtaatgg tgggtgtttt ggggattttc aatggatttg gtcgcttgct
    42721 ctgggcgagt ttgtctgact atatcggtcg ccctctaacc tttagtatat tactgcttgt
    42781 caatcttttc ttttctctct cactttggct ctttacagat tccgttttat ttgtagttgc
    42841 tatgtctatt ttgatgactt gctatggagc tggtttttct ttgattccag cttatctcag
    42901 tgatattttt ggaaccaagg aattggccgc tctgcatggt tatattttaa cagcttgggc
    42961 aatggctggt ttagcgggac ctattttatt agcagagact tataaaatgg ctcattcgta
    43021 cacacaaacc ttgttcgttt ttctcatttt atacagtatc gccttggctt tgtcttatta
    43081 tctaggtcgt tcaatcaaaa aagaaagtca aaaaccgctt acatgatttt ctgtagaaat
    43141 ggggctatct tttgctctgg tcttgtgtta tactagatag gttgcaaaga aaatagtact
    43201 tttcttttgt ggaaaagaag caacacgatt ttatatctag ttatatgaaa atacgtcata
    43261 aaaagaaaag tataaactaa gccctatagg gtggcttcgc accaccttta gaaagaagaa
    43321 taacgtgaaa tttaatgaat taaacttgtc tgctgatttg ctagcagaaa ttgaaaaagc
    43381 tggttttgta gaagctagtc ctatccaaga acaaactatt cctttggccc ttgaaggcaa
    43441 ggatgttatc ggtcaagctc agactggtac aggaaaaact gcagcctttg gcttgcctac
    43501 ccttgaaaaa atccgtacag aagaagcgac tatccaagcc ttggtcatcg ctccaactcg
    43561 tgaactagct gtccaaagtc aagaagaact cttccgcttt ggtcgtagta agggagtcaa
    43621 agtccgttca gtatatggcg gatcaagcat tgaaaaacaa attaaggctc ttaaatctgg
    43681 tgcccatatc gtggtgggaa ctccaggtcg cctcttggac ttgattaaac gcaaggcctt
    43741 gaaattacaa aacattgaaa cccttatcct tgacgaagcg gatgaaatgc ttaacatggg
    43801 cttccttgaa gacatcgaag ccattatttc ccgtgtacct gagaaccgtc aaactttgct
    43861 tttctcagca actatgccag atgccatcaa acgtatcggt gttcagttta tgaaagcccc
    43921 tgaacatgtc aagattgcgg ctaaggaatt gacaacagaa ttggttgacc agtactatat
    43981 ccgtgttaag gaacaagaaa aatttgacac catgactcgt ctcatggatg tggcacaacc
    44041 agaactcgct attgtatttg gtcgtaccaa acgccgtgtg gatgaattga ctcgtggttt
    44101 gaaaattcgt ggcttccgtg cagaaggaat tcatggcgac ctagaccaaa acaaacgtct
    44161 tcgtgtcctt cgtgacttta aaaatggcaa tcttgatgtt ttggttgcga cagacgttgc
    44221 agcgcgtggt ttggatattt caggtgtgac ccatgtctac aactacgata ttccacaaga
    44281 tcctgagagt tatgttcacc gtatcggtcg tacaggtcgt gctggtaagt caggtcaatc
    44341 tattactttt gttgctccaa acgaaatggg ttaccttcaa atcattgaaa acttgactaa
    44401 gaaacgcatg aaaggtctca aacctgcaag tgtagaagaa tccttccaat caaaaaaaca
    44461 ggtagctctc aagaaaatcg aacgtgattt tgcagatgaa accattcgtg ccaactttga
    44521 gaaatttggt aaggatgctc gcaaattggc tgctgagttt actccagaag aattggcaat
    44581 gtatatcttg agtctgacag tccaagaccc agatagcctt ccagaagtgg agattgcacg
    44641 tgaaaaacca ctaccgttta aaccatcagg taatggtttc ggtggtaaag ctaagggagg
    44701 tcgtggaggc cgtcgtgggg acgaccgtcg agagcgtgat cgccgtggca atggtcgccg
    44761 tgatgagttc aaaaaaggaa gtcgtggcaa cgatcgtttt gataaggaaa aacgttaccg
    44821 taaggataat aaaaaaccac gcaatacttt aagcgaaaag caaacaggct ttgttattcg
    44881 taacaaaggc gataaataag aaaaagcggt tcaaaatgaa tcgctttttt atataaggag
    44941 accgaatgtg aaaagacaaa taaaattatt atattatctt ttataatgtt atgatacaca
    45001 aaaataggca acttgtcaat agaaaaggaa caatttaatt aaaaatattc tggtttttaa
    45061 ttaaattgta agtcaatgca agtcaataag gaattttcag ataattattg aaaaagagac
    45121 gtttttatgt tataatgata gcgattaaat tcgaggtgaa aaatggcaca tttattagaa
    45181 aaaactagaa aaattacatc aattttgaaa cgctcagagg agcagttgca ggatgagctc
    45241 ccttacaacg ctattacgcg tcagttagcg gatattattc attgcaatgc ctgcattatc
    45301 aatagtaagg gacgtctgct tggctatttt atgcgttata aaacaaatac agatcgcgta
    45361 gagcaattct tccaaactaa gattttccca gatgactacg ttcaaggggc taatatgatt
    45421 tacgaaacag aagcaaactt gcctgttgag catgatatga gtattttccc tgttgagagt
    45481 agagatgatt ttccagatgg cttgacgact attgcaccga ttcatgtatc ggggattcgc
    45541 cttggttctt tgattatttg gcgtaatgat aaaaaattcg aagatgagga cttggttctt
    45601 gttgagattg ccagtaccgt tgttgggatt cagcttctta acttccaacg tgaagaagat
    45661 gagaaaaata ttcgtcgtcg tactgctgtc accatggcgg tcaataccct ttcttactcc
    45721 gaacttcgtg ctgtttcagc aattttaggg gaattaaatg gaaatgaagg gaagttgact
    45781 gcgtcagtga ttgcagatcg tatcggaatc actcgctctg tgattgtcaa tgctcttcgt
    45841 aaacttgagt ctgcggggat tattgaaagt cgctcacttg gaatgaaggg aacctatctt
    45901 aaggtcttga tttcagatat ttttgaagaa gtgaagaaaa gagattacta atgacaaagg
    45961 ctttaatttc gattgattat acagaagatt ttgttgctga tagtgggaag ctgacagcag
    46021 gtgctccagc tcaggcgatt tcggatgcca tcagcaaggt aactcgatta gcttttgaac
    46081 gaggagatta tatcttcttt actattgatg ctcatgaaga aaacgattgt ttccatccgg
    46141 aaagtaagtt atttcctcct cataatctga ttgggacgag tggacggaat ttatatggag
    46201 atttggggat cttttatcaa gagcatggtt cagacagtcg tgtcttttgg atggataaac
    46261 gccattactc agctttttca gggactgacc tagatattcg tttgagagag cgtagagtgt
    46321 ctaccgttat cttaacaggt gtcttgacgg atatctgtgt cctacataca gctatagatg
    46381 cctataatct aggatatgac atcgagattg ttaaaccagc tgttgcttcc atctggcctg
    46441 aaaatcatca atttgcccta ggtcatttca aaaatacact cggagctaag ttagtagatg
    46501 aaaatctaaa tgaactttct gagtaacctg gttggtgaaa atgaaaaaat ttgaaaaaaa
    46561 gtgttgacaa gcatcccaaa agttgatata ctagtaaagt aatcgatgcg gggatggcgg
    46621 aattggcaga cgcgcaggac taaggatcct gtgaccgctt taggtcgtga gggttcaagt
    46681 ccctctctcc gcataggaaa agagttagct aaagctagct cttttgtatt tctctaaaaa
    46741 attagaatcc agtcaaatgt agtggagaat cagcgaaaat atgggaaaac tgatccttgt
    46801 ttctgctttt gtcatagaag aataataaag attatcttct tcaaattctc cgatattctc
    46861 taaagttttg tgcaagttgc acagaacttt tttatttttt tggtcatctt gccatagaaa
    46921 tataaagcgt tttcatctat aatataatta tcaaaagaca aaaggagttc acctcatggt
    46981 agaattgaat cttaaaaata tttacaaaaa atatccaaac agcgaacact attcagttga
    47041 agatttcaac ttgaacatca aagataaaga atttatcgtt ttcgtaggac cttcaggatg
    47101 tggtaaatca actacactcc gtatgattgc tggtcttgaa gacatcacag aaggtactgc
    47161 atctatcgat ggcgtggttg tcaacgacgt agctccaaaa gaccgtgata tcgccatggt
    47221 attccaaaac tacgctcttt acccacacat gactgtttat gacaacatgg ctttcggttt
    47281 gaaattgcgt aaatacagca aagaagacat taacaaacgt gttcaagaag cagctgaaat
    47341 acttggattg aaagaattct tggaacgtaa accagctgac ctttcaggtg gtcaacgtca
    47401 acgtgttgcc atggggcgtg cgattgtccg tgatgcgaaa gtattcttga tggacgaacc
    47461 tttgtcaaac ttggatgcca aacttcgtgt atcaatgcgt gctgaaatcg ctaaaattca
    47521 ccgtcgtatc ggagctacaa ctatctatgt aactcacgac caaacagaag cgatgacact
    47581 tgcagaccgt atcgttatta tgtcagctac taagaaccct gctggtacag gtactatcgg
    47641 acgtgtagaa caaatcggta ctcctcaaga agtttacaaa aatccagtta acaaatttgt
    47701 tgcaggattc atcggaagcc cagctatgaa tttcatcaac gtgaaattgg ttggtagcga
    47761 aattgtttct gacggtttcc gtttgaaagt gccagaagga gcattgaaag ttcttcgtga
    47821 aaaaggctac gaaggaaaag aattgatctt tggtatccgt ccagaagacg tgaatgcaga
    47881 acctgctttc cttgaaacat tcccagactg tgttgtaaaa gcgactatct ctgtatcaga
    47941 actgcttggt tcagaatctc acctttactg ccaagttggt aaagacgagt ttgttgcaaa
    48001 agttgatgct cgtgactact tgcaaacagg tgcaacagtt gagcttggat ttgacttgaa
    48061 caaagcacac ttcttcgatg tagaaactga gaaaacaatt tactaaaata aataaaattc
    48121 aaagcactac aagaaaagat atctctttat caattgtagt ggagagatat cagttaatct
    48181 agggagagaa acaaaatgct tctctccttt ttgctaggga agtcatatta tgcatctata
    48241 ttgtgatgct ctttaatact cttcgaaaat ctcttcaaac cacgtcaacg tcgccttgcc
    48301 gtacgtatga ttactgattt cgtcagtttt atctgcaacc tcaaagatgt actttgagca
    48361 gcttacggct agtttcctag tttgctcttt gatttccatt gagtattatt tgtgggtacc
    48421 atctacaagt gaagctatat gcgtaaacta cgtgagcaat tgaattcgaa ctagagaggt
    48481 aataataaat ttatgctata gttatggtga cttgtatgct tttgattcta gtttatcaaa
    48541 taatagatta gaattgtcag ataatatcat tttgtgttat aatgaagaaa aaacagaggt
    48601 gtgcaaatgt cagaagcagg tcataagttt ttagcaaaat tggggaaaaa acgcttacgt
    48661 ccaggtggaa agcgtgccac agattggtta attgcagaag gaggattttc aaaagaaaag
    48721 agaatactag aggttgcgtg taatagggga actacagcaa ttgagttggc acagcgtttt
    48781 ggttgcaaga taactgctgt tgatatggat gctcaagctt tagaagtggc taaaaaatct
    48841 gctggaacgg caggtgttgc tcatttaatc agttttgaaa gagcaaatgc aatgaaactt
    48901 ccttatcaag atgctagttt tgatattgtt ataaatgaag ctatgctgac tatgcaagcc
    48961 gatcaagcta agaaaaaatg tgtaatggaa tatctaaggg tattaaaacc tggaggtctt
    49021 cttttgacac atgatgtgct tcttaaggaa gctaaagagt ctatcagaca ggaattatca
    49081 caagcaattc atgtaaatgt aggtccttta actcaagatg gttgggaaca ggtgatgata
    49141 gaatcaggtt attgtgatgt gaaagtattg actggtgaaa tgacattaat gaaattatcg
    49201 ggtatgattt atgacgaagg ttggctagga actttgaaaa tttgtgtaaa tgcttgtaaa
    49261 aaggagaata gaaagcagtt tttaactatg tataaaatgt ttgctaagaa taaacagaaa
    49321 ttgggcttta ttgcgatggc tagttataaa tcgtcaaaac gttagataat tattgaagtt
    49381 aacttttcct tttttctttc ttaaaaaata tgctataata gagagtaaaa aactttgaaa
    49441 gaaagaaaaa gatgaattta aaagattaca ttgcaacaat tgaaaattat ccaaaggaag
    49501 gcattacctt ccgtgatatt agtcctttga tggctgatgg aaatgcttat agctacgctg
    49561 ttcgtgaaat cgttcagtat gctactgaca agaaagtcga catgatcgtg ggacctgaag
    49621 ctcgtggatt tatcgtgggt tgtccagttg cctttgagtt gggaattggt tttgcgcctg
    49681 ttcgtaagcc aggtaaattg ccacgcgaag ttatttctgc tgactatgaa aaagagtacg
    49741 gtgtcgatac cttgactatg cacgcggatg ccattaagcc aggtcaacgt gttcttattg
    49801 tagatgacct tttggcgaca ggtggaactg ttaaggcaac tatcgagatg attgaaaaac
    49861 ttggtggtgt tatggcaggt tgtgccttcc ttgttgaatt ggatgaattg aacggccgtg
    49921 aaaaaattgg tgactacgac tacaaagttc ttatgcatta ttaatgaaaa cagtccctag
    49981 ggctgttttc tctacactag gatataaaaa tagactataa ctagttagag aaaaactata
    50041 attgaaaact atatcttctt gcagtataat aaaaggacta agtgtttgag atttgtcttc
    50101 aaacatatgc aattattcct gaaagagtac agttaggaga gggttatgcc gattcgaatt
    50161 gataaaaaat tgccagctgt tgagatttta cggacagaga atatctttgt catggatgat
    50221 caacgtgctg cccaccaaga tatccgtcct ttgaagattt taattttaaa tctcatgcca
    50281 cagaaaatgg tcacagagac ccagttgttg cgccacttgg ctaatacacc cctacaactg
    50341 gatattgatt ttctctatat ggagagccac cgttctaaaa caactcgttc agagcacatg
    50401 gagaccttct ataaaacttt tcctgaagtc aaggatgagt attttgatgg gatgatcatc
    50461 acgggtgctc cagttgagca tttaccattt gaggaagtgg actattggga ggaatttaga
    50521 cagatgcttg agtggtctaa gactcatgtc tattcgaccc ttcatatctg ttggggggct
    50581 caggctgggc tttatctgcg ctatggtgta gaaaaatacc agatggacag taagctatca
    50641 ggtatttatc ctcaggacac cctaaaagag ggtcaccttc tatttagagg ctttgatgat
    50701 agctatgtat cccctcattc acggcacacg gagatttcta aggaagaggt cttaaacaag
    50761 accaatctcg agattttatc agaaggacct caggttgggg tttctatttt ggccagtcgt
    50821 gatttacgag aaatttatag ttttggtcat ttggagtatg accgtgatac cttggcaaaa
    50881 gagtattttc gagatcgtga tgcaggtttc gacccacata ttccagaaaa ttactttaaa
    50941 gatgatgatg ttaatcaggt accttgtctt tgttggtctt catctgcagc cctctttttc
    51001 agtaattggg taaaccatgc ggtctatcag gagacgcctt ttgactggag aaagatagaa
    51061 gatgatgcat ctgcatatgg gtatttataa gaggaattat gacatattta gacgctttta
    51121 aatcaggtgc cttggtttta ccgagtgccc tgctcttgca ttttaaggaa ctctttcctt
    51181 ctagcgacga ttttctggtt tggcaatttt tctatttgca aaatacgaca ggcttagaag
    51241 aaatgtcgcc aagccagatt gctgaaagga ttggcaagga aatttcggat gtcaaccagt
    51301 ccatttctaa tctgacggaa aggggactgc tccagtatcg tactatcgaa ttaaatggcg
    51361 aaattgaatt gctctttgat gctagtttgg ccttggaacg tttggatgac ctgtttggag
    51421 cagttcattc aagttcagac cagctaacac ctcaaaacca gctcaaggat ttggtggaaa
    51481 ccttccagca ggagttggga cgattgttga cgccttttga gattgaggat ttgaccaaga
    51541 cactaaagga agatggaacc agtgctgact tgattaagga ggctcttcgt gaagctgttt
    51601 tgaatggaaa accaaactgg aagtacattc aggcgatttt gagaaactgg cgccatgaag
    51661 gaatcaagag tgtggctcaa attgaggcca agagagcaga aagagaagca agcaatcctc
    51721 agttgacaca ggtatctgca gatttcataa atgccatgga tctctggaag gattaatcca
    51781 tgcaagtagg cttgaaatcc gagtaagatt tgcaagctgt gtataattgt gatagaataa
    51841 atagaaaata aattgaaaaa agaggtatgt gaaatgtcac gtaaaccatt tatcgctggt
    51901 aactggaaaa tgaacaaaaa tccagaagaa gctaaagcat tcgttgaagc agttgcatca
    51961 aaacttcctt catcagatct tgttgaagca ggtatcgctg ctccagctct tgatttgaca
    52021 actgttcttg ctgttgcaaa aggctcaaac cttaaagttg ctgctcaaaa ctgctacttt
    52081 gaaaatgcag gtgctttcac tggtgaaact agcccacaag ttttgaaaga aatcggtact
    52141 gactacgttg ttatcggtca ctcagaacgc cgtgactact tccatgaaac tgatgaagat
    52201 atcaacaaaa aagcaaaagc aatctttgcg aacggtatgc ttccaatcat ctgttgtggt
    52261 gaatcacttg aaacttacga agctggtaaa gctgctgaat tcgtaggtgc tcaagtatct
    52321 gctgcattgg ctggattgac tgctgaacaa gttgctgcct cagttatcgc ttatgagcca
    52381 atctgggcta tcggtactgg taaatcagct tcacaagacg atgcacaaaa aatgtgtaaa
    52441 gttgttcgtg acgttgtagc tgctgacttt ggtcaagaag tcgcagacaa agttcgtgtt
    52501 caatacggtg gttctgttaa acctgaaaat gttgcttcat acatggcttg cccagacgtt
    52561 gacggtgccc ttgtaggtgg tgcgtcactt gaagctgaaa gcttcttggc tttgcttgac
    52621 tttgtaaaat aatcagtaag tagcaaaagc taggtggaac agcattcaga tgtctgttac
    52681 attttttata ggagagaaag attgaaaaca aaaattggat tagcaagtat ctgtttacta
    52741 ggcttggcaa ctagtcatgt cgctgcaaat gaaactgaag tagcaaaaac ttcgcaggat
    52801 acaacgacag cttcaagtag ttcagagcaa aatcagtctt ctaataaaac gcaaacgagc
    52861 gcagaagtac agactaatgc tgctgcccac tgggatgggg attattatgt aaaggatgat
    52921 ggttctaaag ctcaaagtga atggattttt gacaactact ataaggcttg gttttatatt
    52981 aattcagatg gtcgttactc gcagaatgaa tggcatggaa attactacct gaaatcaggt
    53041 ggatatatgg cccaaaacga gtggatctat gacagtaatt acaagagttg gttttatctc
    53101 aagtcagatg gggcttatgc tcatcaagaa tggcaattga ttggaaataa gtggtactac
    53161 ttcaagaagt ggggttacat ggctaaaagc caatggcaag gaagttattt cttgaatggt
    53221 caaggagcta tgatgcaaaa tgaatggctc tatgatccag cctattctgc ttatttttat
    53281 ctaaaatccg atggaactta tgctaaccaa gagtggcaaa aagtgggcgg caaatggtac
    53341 tatttcaaga agtggggcta tatggctcgg aatgagtggc aaggcaacta ctatttgact
    53401 ggaagtggtg ccatggcgac tgacgaagtg attatggatg gtactcgcta tatctttgcg
    53461 gcctctggtg agctcaaaga aaaaaaagat ttgaatgtcg gctgggttca cagagatggt
    53521 aagcgctatt tctttaataa tagagaagaa caagtgggaa ccgaacatgc taagaaagtc
    53581 attgatatta gtgagcacaa tggtcgtatc aatgattgga aaaaggttat tgatgagaac
    53641 gaagtggatg gtgtcattgt tcgtctaggt tatagcggta aagaagacaa ggaattggcg
    53701 cataacatta aggagttaaa ccgtctggga attccttatg gtgtctatct ctatacctat
    53761 gctgaaaatg agaccgatgc tgagagtgac gctaaacaga ccattgaact tataaagaaa
    53821 tacaatatga acctgtctta ccctatctat tatgatgttg agaattggga atatgtaaat
    53881 aagagcaaga gagctccaag tgatacaggc acttgggtta aaatcatcaa caagtacatg
    53941 gacacgatga agcaggcggg ttatcaaaat gtgtatgtct atagctatcg tagtttatta
    54001 cagacgcgtt taaaacaccc agatatttta aaacatgtaa actgggtagc ggcctatacg
    54061 aatgctttag aatgggaaaa ccctcattat tcaggaaaaa aaggttggca atatacctct
    54121 tctgaataca tgaaaggaat ccaagggcgc gtagatgtca gcgtttggta ttaagcgatg
    54181 atttgaaaga gggatgtgat agtagcaccc tctttttctt tgttttatga tagttcatcc
    54241 tcgagtaaat tcaagttctt gctcggaaat gaagcttata tagtagattg aatatagaca
    54301 aataccttgt gattggtaaa acattttaga aattcattta cctttcctaa tcgacttggt
    54361 ttcatcttat ttcaatctat tatagtattg gggaatttct tcaaaccaca tcagcttggt
    54421 cagttctacc tgcgacctca aaacttgtgc tttggtcaag ctgggtttag tttcctagtt
    54481 tgctgatgga tttccattga ctataagcat ccaaccctct ttttgtcttc taaagaattc
    54541 ttaaattatc agtctattgc aacttttctc atataagttc tttgtcttgc tattggtttt
    54601 ccttagtagt atactaaggt agtaatcatt aagaagtggt tacaaaaaat aatgaatgag
    54661 gtaaagaaaa tggtagaatt gaaaaaagaa gcagtaaaag acgtaacatc attgacaaaa
    54721 gcagcgccag tagcattggc aaaaacaaag gaagtcttga accaagctgt tgctgatttg
    54781 tatgtagctc acgttgcttt gcaccaagtg cactggtata tgcatggtcg tggtttcctt
    54841 gtatggcatc caaaaatgga tgagtacatg gaagctcttg acggtcaatt ggatgaaatc
    54901 agtgaacgct tgattacact cggtggaagc ccattctcta cattgacaga gttccttcaa
    54961 aatagtgaaa tcgaagaaga agctggtgaa taccgtaatg ttgaagaaag cttggaacgt
    55021 gttcttgtta tctaccgtta cttgtcagaa cttttccaaa aaggtttgga tgtcactgat
    55081 gaagaaggtg acgatgtgac aaacggtatc tttgcaggcg ctaaaactga aacagataaa
    55141 acaatttgga tgcttgcagc cgaacttgga caagcacctg gtttgtaaga aataaggaaa
    55201 catagataaa tctgtaggaa atttcttaca gattttttct attttgtaag ctattccaaa
    55261 ccagtctttt tttgagtttt ttgataaaat agtactatca gtgaaaagga tggaagcatg
    55321 actaagaaaa tcgtagctat ttgggcccag gatgaagagg gtgtgattgg taaggaaaat
    55381 cgtctgcctt ggcatttgcc agcagaattg cagcacttta aagaaacaac tctgaatcat
    55441 gctatcttga tggggcgtgt gacctttgat gggatggggc gtcgcttgct tccaaaacgg
    55501 gaaaccttga ttttgacgcg taatccggaa gaaaagatag atggggttgc tacttttcag
    55561 gacgtccagt ctgttcttga ctggtatcag gctcaagaaa agaatctcta cattatcggt
    55621 gggaagcaaa tttttcaggc ttttgaacct taccttgatg aagtgattgt cactcacatt
    55681 catgctcggg tggaaggaga tacctatttc cctgaagaac ttgacttgtc tctttttgag
    55741 acagtttcaa gcaaatttta cgccaaagat gagaagaatc cttatgattt taccatccaa
    55801 taccgcaaga gaaaggaagt ctaatggaac ggagtttatt tggtctattc acggcttttc
    55861 tgtgtttcat ctgctttctg gcaggagcac aagcttttcg taaaaagcgt tacgggcttt
    55921 ctatcctcct ttggttaaat gcctttacca atctggtcaa tagtattcat gctttttaca
    55981 tgaccttatt ttagatagaa tgacagtata gaatggaagg aaatcatgtc tacaaataga
    56041 aaaaatgata tgatggttta ttgctcattt tgtggcaaaa accaagaaga agtacaaaaa
    56101 ataattgctg gcaacaatgc ttttatttgt aatgaatgcg tggagttagc tcaggaaatc
    56161 attcgagaag aattggttga ggaagtcttg gcagacttgt ctgaggtgcc aaaaccaatt
    56221 gaactcctcc atatcttgaa ccactatgta attggtcaag atcgtgccaa gcgtgccttg
    56281 gcagtggcgg tttataacca ctacaaacgc atcaatttcc acgatacacg cgaagagtca
    56341 gaagatgtgg atttgcagaa gtcaaacatt ttgatgattg gcccaactgg ttcagggaaa
    56401 actttccttg cccagacctt ggctaagagc ttgaatgtac cttttgctat tgcggatgcg
    56461 acagctctga cggaggctgg ttatgtgggt gaggatgtgg aaaatatcct cctcaaactc
    56521 ttgcaggttg ctgactttaa catcgaacgt gcagagcgtg gcattatcta tgtggatgaa
    56581 attgacaaga ttgccaagaa gagtgagaat gtgtctatca cacgtgatgt ttctggtgaa
    56641 ggggtgcaac aagcccttct caagattatt gagggaactg ttgctagcgt accgcctcaa
    56701 ggtggacgca aacatccaca acaagagatg attcaagtgg atacaaaaaa tatcctcttc
    56761 atcgtgggtg gtgcttttga tggtattgaa gaaattgtca aacaacgtct gggtgaaaaa
    56821 gtcatcggat ttggtcaaaa taataaggcg attgacgaaa acagctcata catgcaagaa
    56881 atcatcgctg aagacattca aaaatttggt attatccctg agttgattgg acgcttgcct
    56941 gtttttgcgg ctcttgagca attgaccgtt gatgacttgg ttcgcatctt gaaagagcca
    57001 agaaatgcct tggtgaaaca ataccaaacc ttgctttctt atgatgatgt tgagttggaa
    57061 tttgacgacg aagcccttca agagattgct aataaagcaa tcgaacggaa gacaggggcg
    57121 cgtggacttc gctccatcat cgaagaaacc atgctagatg tcatgtttga ggtgccgagt
    57181 caggaaaatg tgaaattggt tcgcatcact aaagaaactg tagatggaac ggataaaccg
    57241 atcctagaaa cagcctagag gtgactatgg aacttaatac acacaatgct gaaatcttgc
    57301 tcagtgcagc taataagtcc cactatccgc aggatgaact gccagagatt gccctagcag
    57361 ggcgttcaaa tgttggtaaa tccagcttta tcaacactat gttgaaccgt aagaatctcg
    57421 cccgtacatc aggaaaacct ggtaaaaccc agctcctgaa cttttttaac attgatgaca
    57481 agatgcgctt tgtggatgtg cctggttatg gctatgctcg tgtttctaaa aaggaacgtg
    57541 aaaagtgggg gtgcatgatt gaggagtact taacgactcg ggaaaatctc cgtgcggttg
    57601 tcagtctagt tgaccttcgt catgacccgt cagcagatga tgtgcagatg tacgaatttc
    57661 tcaagtatta tgagattcca gtcatcattg tggcgaccaa ggcggacaag attcctcgtg
    57721 gtaaatggaa caagcatgaa tcagcaatca aaaagaaatt aaactttgac ccgagtgacg
    57781 atttcatcct cttttcatct gtcagtaagg cagggatgga tgaggcttgg gatgcaatct
    57841 tagaaaaatt gtgaggaaaa gaaaatggca aaaacaattc atacagataa ggccccaaag
    57901 gctatcggac cctatgttca aggaaaaatc gttggcaacc ttttgtttgc tagcggtcaa
    57961 gttcccctat cccctgaaac tggggaaatt gtaggagaga atatccaaga acagacagag
    58021 caagtcttga aaaacatcgg tgctattttg gcagaagcag gaacagactt tgaccatgtt
    58081 gtcaaaacaa cttgcttctt gagcgatatg aacgactttg ttccttttaa tgaggtttac
    58141 caaacggcct tcaaagagga attcccagct cgttcagctg tggaggtagc tcgtcttcct
    58201 cgtgatgtaa aagtcgaaat tgaagtcatc gcagagattg gataagctag ttgaagtttg
    58261 gtgttgccaa acttcttttg atataaggag aaaaagatga caaagaaaca acttcacttg
    58321 gtgattgtga cagggatgag tggcgcaggg aaaactgtag ccattcagtc cttcgaggat
    58381 ctaggttatt tcaccattga taatatgccg ccagctctct tgcctaagtt tttgcagctg
    58441 gttgaaatta aggaagacaa tcctaagttg gccttggtag tggatatgcg tagccgttct
    58501 ttcttttcag agattcaagc tgttttggat gagttggaaa atcaagatgg tttggatttc
    58561 aaaatcctct ttttggatgc ggctgataag gaattggtcg ctcgttacaa ggagaccaga
    58621 cggagtcacc cactagcagc agacggtcgt attttagatg gaatcaagtt ggaacgtgaa
    58681 ctcttggcac ctttgaaaaa tatgagccaa aatgtggtgg atacgactga actcactcca
    58741 cgtgagctgc gcaaaaccct tgcagagcag ttttcagacc aagaacaagc ccagtctttc
    58801 cgtatcgaag tcatgtcttt cggatttaag tatggaatcc cgattgatgc ggacttggtc
    58861 tttgatgtcc gtttcttgcc aaatccctat tatctaccag aactgagaaa ccaaacgggt
    58921 gtggatgaac ctgtttatga ttatgtcatg aaccatcctg agtcagaaga cttttatcaa
    58981 catttattgg ccttgattga gccgattctg ccaagttacc aaaaggaagg taagtccgtt
    59041 ttgaccattg ccatgggatg tacgggtgga caacaccgta gtgtggcatt tgctaaacgc
    59101 ttggcgcagg acttatccaa gaattggtct gttaatgaag ggcatcgcga caaagaccgc
    59161 agaaaggaaa cggtaaaccg ttcatgagaa aaccaaagat aacggtgatt ggtggaggga
    59221 ctggaagtcc cgtcattcta aaaagtctgc gggaaaaaga tgtggaaatc gcagctatcg
    59281 tgacggtggc agatgatggt ggttcttcag gtgaactccg aaaaaatatg caacagttga
    59341 caccgccagg tgatcttcgt aatgtccttg tggccatgtc ggatatgcct aagttttatg
    59401 agaaggtctt tcagtatcgg ttctctgagg atgccggagc ctttgctggc catccattgg
    59461 gaaatctcat cattgctggc ttgtcagaaa tgcagggttc aacctataat gccatgcagt
    59521 tattgagcaa atttttccat acaacaggga aaatttatcc ttccagtgac catcctttga
    59581 cccttcatgc agtctttcag gatgggacag aagtggctgg agagagtcat attgtagacc
    59641 atcgaggcat aattgacaat gtctatgtga ccaatgccct aaacgatgat acgcctctgg
    59701 ccagccgtcg agtagtgcag accatccttg aaagtgacat gattgtccta gggccaggtt
    59761 ccctctttac ctctattttg cccaatatcg tgattaagga aattgggcgg gctcttttgg
    59821 aaaccaaggc agaaattgcc tatgtctgca atatcatgac ccaacgtggg gagacggaac
    59881 actttacaga tagcgaccac gtggaagtct tgcatcgtca ccttggtcgc ccttttatcg
    59941 acactgtctt ggtgaatatt gaaaaagtgc ctcaggaata catgaattcc aaccgttttg
    60001 atgaatactt agtgcaagtg gaacacgatt ttgtaggtct ttgtaagcaa gtttcgcgcg
    60061 tgatttcatc taacttcctt cgtctggaaa atggcggtgc cttccacgat ggagatttga
    60121 ttgtggacga gttgatgcgc attatacagg tgaaaaaatg agtttcacag tagcagtaaa
    60181 agaagaaatc ctgggccaac accatctgag ctggcatgaa ttatctgcca ttatcaaaat
    60241 gtctggtagc atcggtctct cgacttcggg cttgactttg tcagttgtga cagaaaatgc
    60301 caaactggcc cgtcacctct atgagtcctt tctccatctc tatgaaatca aatctgaaat
    60361 tcgtcaccac caacggagca atcttcgcaa gaatcgcgtc tataccgttt ttacagatga
    60421 aaaggtgcag gatttgttaa gtgatttgca cttggcagac tctttctttg gcctggaaac
    60481 aggtattgat gaggcgattt tatcggatga ggaagcaggt cgtgcctatc tctgtggcgc
    60541 tttcttggca aatgggagca ttcgtgaccc tgagtcaggc aagtaccagt tggaaatcag
    60601 ttctgtttat ctggaccacg cgcaaggaat tgcctccctt ctccaacagt ttttactgga
    60661 tgccaaggtg cttgagcgca agaagggggc tgtgacctat ctccagcgag cagaagacat
    60721 tatggacttc ttgatagtaa taggagccat gcaggcacgt gatgattttg agcgggttaa
    60781 gattttgcga gaaacccgta acgacctcaa tcgggctaat aatgccgaga cagctaatat
    60841 cgctcggaca gtttctgcca gcatgaagac cattaacaat atcagtaaaa tcaaagatat
    60901 catgggctta gaaaatttgc cagtagattt gcaggaagtg gcgcaactga ggattcagca
    60961 cccagactac tctatccagc agttagcaga tagtctcagc acccctctga ccaaaagtgg
    61021 tgtcaatcac agactcagaa aaatcaataa gatagcggat gaattataaa accacgaatc
    61081 ctatgtgact cgtggttctt ttttataaac tggtagagtg ttttggttgt actttttgtt
    61141 cagggtcaat gtagttgata gcgttgttga cagcagttgg agcttctccg aggcctgtcg
    61201 caatcagatc aatttttccg tcatagtagc agcagtcacc gatagcatag atacctgctt
    61261 ggctggattc ctgtttgctg ttgacgataa tcttgtgacg gttgaggtcg agcccccagt
    61321 ttttaaggtt accgacagaa gatttgaaac catagttgac aaagaggtgg tctagatcaa
    61381 tggtttcagt ttcatcagat ttgacttttg tgatttcaag tttatcaagt gtttttccat
    61441 ttccaaggag ttggctaggg gcgaatggtg tcttgatggt tacagatgat tcttgcaagg
    61501 cttgaacact gtgttccaag gcacggaaat tatctctgcg gtgaacaagg gtagttggtg
    61561 cgattttttc aaaagccaaa gcccaatcca cagccgagtc tcccccacca agaatcgtca
    61621 ctttcttacc agcgtattgc tgaatgttag aaacgtggta gtggatattt tcatagccct
    61681 caaccccttc aagttccagc ggacgtggtt tgaaggcacc gccacccata gcgatgataa
    61741 ctgttttagt caggtgactt cctttagaag ttgtgatggc aaattcttct tcttgtttgt
    61801 caatctcaag aaccgtttca ttgagatgaa taggggtatc aaatccattt agctgttcaa
    61861 tcaagcggtt agtcaactct tctccagtca ggtttgggaa gcctggtacg tctaggattt
    61921 ccttttcagg gtagagaata gcaggttgtc cacctagctg gggaagagag tcgatgattt
    61981 gaaccttggc ttggcgtagg tgggcataaa aggctgcaaa aagcccgaca ggaccaccac
    62041 ccacaatggt aatatcatag agttgagaca tagtttctcc tttgtttttt ctagtcagtt
    62101 tattttatca tatttttgtt caatgtaaaa tggactagga tagggaaaaa aatgccagaa
    62161 aatggtataa taggacgaaa gtgttttgat agggaggata tcagggatga attttcaaca
    62221 attatccaat ctgcaatatt ggaccagttt gtttgcaagt ccatggacga tagctatcaa
    62281 tctgattgat attttgattg ttgcttatat tttataccat tttacaaaag ctattgcagg
    62341 aaccaagatt atgattttgg tacgtggagt tttggtgttt attttagctc aaatccttgc
    62401 aaatatgatt ggtttgacta cgatttcttg gttaatcaat caaattatta cttatggggt
    62461 tattgcggcg gttgttatct tctctccaga gattcggact ggtttggaac gtttgggaag
    62521 agcgacagat ttcttttcca atgcccctat tagtgctgag gaacagatga ttcgtgcctt
    62581 tgttaagtct gttgaataca tgagtcctcg taaaatcggg gccttggttg ctattcagcg
    62641 tgtacgtacc ttgcaggagt atacttcgac aggaattccc ttggatgcta agatttctgc
    62701 agaacttctc attaacattt ttattcccaa cactccccta catgatggtg cggtgattat
    62761 caaagaagaa cgtatcgctg tgacgtctgc ctatctgccc ttgacaaaaa acacagggat
    62821 ttccaaggaa tttgggacca gacaccgggc ggctatcggt ttatcagaag tctcagatgc
    62881 cttgactttt gtcgtatcag aggaaacggg aggaatttcg ataacctata atggaaggtt
    62941 taagcacaac ctaacacttg atgaatttga aacagaatta cgtgaaatct tacttccaaa
    63001 agaggaagtg ggtcttagtt ttaaagaacg attgctagga ggatggaaac atgaaaaaaa
    63061 atagtttata tatcatatcc tcactctttt ttgcttgtgt cttatttgtc tatgctacgg
    63121 cgacgaattt tcaaaacagt accagtgcta ggcaggtaaa aacggaaacc tatactaata
    63181 cagtaacaaa tgtccctatt gacatacgct ataatagtga taagtatttt attagcggtt
    63241 ttgcttcaga agtatcagtg gtcttgactg gtgcaaatcg cctatcgcta gctagtgaaa
    63301 tgcaagaaag tacacgtaaa ttcaaggtta ctgctgacct aacagatgcc ggtgttggaa
    63361 cgattgaagt tcctttgagc attgaagatt tacccaatgg gctgaccgct gtggcgactc
    63421 cgcaaaaaat tacagtcaag attggtaaga aggctcagaa ggataaggta aagattgtac
    63481 cagagattga ccctagtcaa attgatagtc gggtacaaat tgaaaatgtc atggtgtcag
    63541 ataaagaagt gtcgattacg agtgaccaag agacattgga tagaattgat aagattatcg
    63601 ctgtcttgcc aactagcgaa cgcataacag gtaattacag tggttcagta cctttgcagg
    63661 caatcgatcg caatggtgtt gtcttaccgg cagttatcac tccgtttgat acaataatga
    63721 aggtgactac aaaaccagta gcaccaagtt caagcacatc aaattcaagt acaagcagtt
    63781 catcggagac atcttcgtca acgaaagcaa ctagttcaaa aacgaattaa aaatagaaaa
    63841 ggattttata acaatgggta aatattttgg gactgatgga gtccgtggag aagctaacct
    63901 agagctaaca ccagaattag cctttaaact aggacgtttt ggaggctatg ttcttagtca
    63961 acatgaaacg gaagcgccga aagtctttgt aggacgtgac acacgtattt caggggaaat
    64021 gctggaatcg gccttggtgg caggtctcct ttcagtaggg attcacgtat acaaacttgg
    64081 tgtccttgca acaccagcag tagcttactt ggttgaaact gaaggagcaa gtgccggtgt
    64141 catgatttct gctagccaca acccagccct tgataacgga atcaagttct ttggcggtga
    64201 tggcttcaaa ctagatgatg aaaaagaagc agaaattgaa gccttgctag atgctgagga
    64261 agacactctt cctcgtccaa gtgcagaagg cttaggaatt ttggtagatt atccagaagg
    64321 cttgcgtaag tatgaaggat accttgtttc aactggaact cctcttgatg gaatgaaggt
    64381 tgccttggat acagctaatg gagcagcttc taccagtgcc cgtcaaatct ttgcagacct
    64441 tggtgcccaa ttgacggtta tcggggaaac accagacggt cttaacatca accttaatgt
    64501 tggttcaaca catccagaag cccttcaaga agtggtcaaa gaaagtgggt cagctattgg
    64561 tttggccttt gatggagaca gtgaccgctt gattgctgtt gatgagaatg gtgacatcgt
    64621 cgatggtgac aagattatgt acatcatcgg aaaatacctt tctgaaaaag gacaattggc
    64681 tcaaaataca attgtgacaa ctgttatgtc taaccttggt ttccacaagg ccttgaatcg
    64741 cgaaggtatt aacaaggcag ttactgcagt tggtgaccgc tacgttgttg aagaaatgag
    64801 aaaatcaggt tacaaccttg gtggtgaaca gtctggtcac gttatcttga tggattacaa
    64861 taccacaggt gatggtcaat tatcagcagt tcaattgact aaaatcatga aggaaactgg
    64921 taagagctta tcagagttgg cggcagaagt aacgatttat ccacaaaaat tagttaatat
    64981 ccgagtggaa aacgtcatga aggaaaaggc catggaagtg ccagctatca aggccatcat
    65041 cgagaagatg gaagaagaaa tggcggggaa cggccgtatc cttgttcgtc caagtggaac
    65101 agaacccctc ttgcgtgtta tggcagaagc gcctacaaca gaagaagtag actactatgt
    65161 tgataccatc acagatgtag ttcgtgctga aattgggatt gactaaatcc tagaaaacta
    65221 gatgaaaata aaaaattatg gtacaatagc ttataatatt gtagccgatg gagaaagaca
    65281 tgaatcttaa acgtgaacaa gaatttgtta gtcagtatca ttttgatgcg cgtaattttg
    65341 aatgggaaaa tgaaaatgga gctcctgaaa ccaaggtgga tgtgaatttc caattgctcc
    65401 aacatgacca agaaaatcaa gtgacttcct tgattgtcat cttgagcttt atgattgtat
    65461 ttgataagtt tgtcattagt ggaacaatct cacaagtaaa ccatatcgat ggccgtattg
    65521 tcaatgaacc aaatgaattg aaccaagaag aggtggaaac cttggctcgt ccatgtttga
    65581 acatgctcaa tcgtttgact tacgaagtga ctgaaattgc attagactta ccaggaatta
    65641 atttggagtt ctaatatgaa attagctgtt ttcacagatt cctctgctta tctcagtgca
    65701 gagatcttgc aaagagaaga cttgtttgtc ttggatattc ctgtcaatat tgatggtgag
    65761 gaatatgtcg aaggcatcaa tctgtctgct gaggaatttt accaaaaaat ggctcaggct
    65821 tctgaattgc ctaagaccag tcaaccaagt attgccaagt tagatgagat cttaacttcg
    65881 ctcaaagaac aaggctatac acatgccttg gggcttttcc tatcttctgg aatttcaggt
    65941 ttttaccaaa atatccagta tatggtcgat gactatgagg gcttaaccat tgctttccca
    66001 gacactttga ttacaagtgc tcccctaggc atcatggttg aaagcgtctt taattggcgt
    66061 gatcagggcg atgattttgc cagcattcag gataagctag ccattcaaat cagccgtacg
    66121 tcagccttta tcatggtaga tgacttggat catttggtaa aaggtggacg cctttcaaat
    66181 ggggctgcca ttttgggcaa tttgcttagc attaagccaa tcctttattt taacgatcaa
    66241 ggtgtgattg aagtttacga aaaagttcgt actgaaaaga aggccaccaa gcgcttaatt
    66301 gaaattatca aggaaacaac ggcttcaggt caataccggg tcattgtcat tcatgggaat
    66361 gctcctgaaa aggctgaaga attgcgtcag cacttgcttg attttggctt gggttcggat
    66421 gtttcacttg ctacatttgg tagtgtcatt ggaacgcacc taggagcagg aagtattgct
    66481 ctgggttata ttccagtgat ttagttggcg cttctaggct agttaagatg ccttgtatct
    66541 tagaaattga acacgcctgg aaccctgtgt gaaaaagata gttcttccaa gaagtagaca
    66601 cttcttgatt ggaattccta ttttcacttt gtgttcttac ggactttgta tcttagacag
    66661 gagtagagat gagtattcga gtaattattg ccggttttaa gggaaagatg ggccaggctg
    66721 cttgtcagat ggtattgact gatccagact tggacttggt ggcagttttg gatccttttg
    66781 agtctgagtc agaatggcag ggtattcctg ttttcaagga taaggctgat ttagctggtt
    66841 ttgaagcgga tgtctgggta gattttacta ctccagctgt tgcctacgaa aatacacgtt
    66901 ttgctcttga aaatggcttt gctccagtag ttggaacgac tggtttcacg agtgaagaaa
    66961 ttgcagagct aaaagaattt tctcgtgccc aagacttggg tggcctgatt gcccctaact
    67021 ttgccttggg tgctgtctta ctcatgcaat ttgcgacgca ggctgccaaa tatttcccaa
    67081 atgtggagat tattgagctc catcatgaca agaaaaagga tgctccgagt ggaacagcca
    67141 ttaaaacagc tgagttgatg gcagaggttc gagagtccat tcagcaaggc gcagcagatg
    67201 aggaagagct gattgctggt gctcgtggtg ctgactttga tggtatgcgc atccactcag
    67261 ttcgtttgcc aggcttggta gctcatcaag aagtcatctt tggcaatcag ggagaagggt
    67321 tgaccctccg tcatgactcc tatgatcgca tctccttcat gacaggagtc aatttgggaa
    67381 ttaaagaagt tgtcaagcgt catgagcttg tctatggatt agaacactta ttatgagatt
    67441 aacgcaaatg ccttctgaat ttcagaaggc tttaccagta ttagaaaaaa ttaaagaagc
    67501 aggctttgag gcttattttg ttgggggctc tgttcgagat gcccttctcc atagccctat
    67561 ccacgatgtg gatattgcga cgtcttctta tccagaagag accaagcaga tttttccgcg
    67621 aacagccgat atcggaatcg agcatggaac cgtcttggtc ttagatgggg atgaggagta
    67681 tgaggtaaca acctttcgga cagaggatgt ctatgtggac tatcgcagac ccagtgcggt
    67741 ttcctttgtg cgctcgctag aagaagacct caaacgccgt gatttcacag tcaacgcctt
    67801 tgccttggat gagacaggag aaatcgttga cttgttccat ggtttagaag atttggaaaa
    67861 gcaagtcttg cgagcagttg gagtggctag tgagcgtttc aacgaagatg ctttacggat
    67921 tatgcgtggt ttccgttttc aggctagtct tggttttgca cttgagccag aaacatttaa
    67981 agctatgaag accttgacgc cgcttttgga gaaaatttct gtagagcgta ccttcgtcga
    68041 gtttgataaa ctcttgctgg ctccattttg gagaaggggc ttggcttcca tgattgagag
    68101 tcaagcttat gactatctcc ctgatatggc atctagccag gacaagctca acagactgtt
    68161 tgatttggag actgatttta cttttgaatc ctctgaacaa gcctgggcgg ctttactatg
    68221 ggctttggag attgaaaatg cgcagtcatt tttgaaatct tggaagacct cacgccagtt
    68281 tgccaagcaa gttcaggatt tgctgattat tttggctctg cgtgaaaatg gagaattgag
    68341 caagcgagat tgttatcgct ttgatataga tttgctttta caggctgaaa atcttcgtca
    68401 agctcaagga aaagaagtca acctacaagc catcacaaaa aaataccaaa gcttgactat
    68461 ccatgacaag aaagagattc agattaatgg cgatattttg atcaaggaat atggctatca
    68521 gccaggccca gacttgggag agattttaac agagattgag tttgccattg tcgatggaga
    68581 attggagaat aatcgtgaag ccatccatgc ttacctgagg gagaaaaaat gagtgatttt
    68641 atcgttgaaa aactaagtaa atccgttggt gacaagaccg tttttaggga tatttccttt
    68701 attatccatg acttagacag aattggttta atcggtgtca atgggactgg caagaccacc
    68761 cttttggacg tcctttctgg tgtttctgga tttgatgggg atgtcagtcc tttttcagct
    68821 aaaaatgatt accagattgg ttacttgact caggatcctg attttgatga tagaaagaca
    68881 gttttggata cggttctatc tagtgaactc aaggaaatcc agctcattcg tgagtatgaa
    68941 ttgattatgc tcgactatag tgaggacaag caggcgcgtt tggaacgtgt catggcagag
    69001 atggactctc tccaagcttg ggaaattgag agtcaggtca agaccgttct tagcaaattg
    69061 ggcattcaag acttatctac tcctgttggg gaattgtcag gtggtctgag aagacgggta
    69121 cagttggcac aagtcttact tggcaaccac gaccttttgc ttttggatga gccgaccaac
    69181 catctggata ttgcgattat tgagtggctg accctctttt tgaaaaattc taagaagacc
    69241 gtccttttta tcactcacga tcgttatttc ttagacgctt tgtcaacacg gattttcgag
    69301 ttggagcgag caggcttgac agaatatcag ggcaattacc aggactatgt tcgccttaag
    69361 gcagaacagg acgagcgcga tgcagctcta cttcataaaa aagaacaact ctacaaacaa
    69421 gaactggcct ggatgcgcag acaaccgcag gcacgtgcga ccaagcaaca agcccgtatc
    69481 aatcgattcc atgatctgaa aaaggaagtt tcaggcagta gtgctgagac agacttgacc
    69541 atgaactttg aaaccagtcg gattgggaag aaagtcatcg agtttcaggc tgtttccttt
    69601 gcctatgaaa acaagcctat tttgcaagat tttaatctct tggtgcaggc caaagaccgt
    69661 atcggaatcg ttggggacaa tggtgttgga aaatcaaccc tgctcaactt gattgcagga
    69721 agtcttgagc caactaaagg acaagttgtg attggggaaa ctgttcgcat cgcctatttc
    69781 tctcagcaaa ttgagggttt ggatgaaagc aagagagtta tcaattacct gcaggaagtg
    69841 gcagaggagg tcaagaccag tggcggttct acgacttcga ttgcagagtt gctggagcaa
    69901 ttcctcttcc cacgttcgac gcatgggacc ttgattgaga aattatctgg tggagagaaa
    69961 aaacgtcttt atctcctcaa actgcttttg gaaaaaccaa atgttcttct tttagacgag
    70021 ccaaccaatg acctagatat tgcaactttg acagtcttag agaatttctt gcaaggtttt
    70081 gcaggtcccg ttttaacagt cagtcacgac cgctatttct tggataaggt agcgaccaag
    70141 attctcgctt ttgaggatgg caagattcgt cctttctttg gtcattacac cgactatctt
    70201 gatgaaaaag cttttgaaac agatatggcc aatcaagtgc aaaaggtcga aaaggaaaaa
    70261 gtggtcaagg ttcgagaaga caagaaacgc atgacctacc aagaaaagca ggagtgggca
    70321 agtattgaag gtgatattga aaccttggaa aaacgtatcg ctgctattga agaggaaatg
    70381 caggctaacg gctctgactt tggtaagctg gctactctcc aaaaagaatt ggatgagaaa
    70441 aatgaagcac tccttgaaaa atacgaacgc tatgagtatc tcagtgaatt tgatagttaa
    70501 gactattcga aaatcaaagt gcaaactatg tcggcttcac attgccgact caagtacagc
    70561 ctgtgaccgg cttcctaatt tgcactttga tttttattga gtataaatga atagaaaaat
    70621 cccgtctcat gaaaggattt ctctattctt ttttggtttc ttgatgaaag atattttgcc
    70681 aggtttcgtg gcgacgccag atactggtat ggatgatacc atctaactca tagcagatga
    70741 gtttggtttt ttgactgatg gaagtaattt gaatgtcctt gatagcagaa tttaactctt
    70801 ttttggtctt ataagcctct ttatccatct gctctccatc ttggcgaata tagacaaaat
    70861 catcagtcaa gagttcttct agttggtttc cttggtcgat caattgttca cgcataagca
    70921 attttttata gacattgtct aaaatttcat cctcagggat aggtgctggt tcgatatgga
    70981 catcggtatc aaagacgcca aaacgattct ccagcataga ctcgacctga tccgcgattt
    71041 catggctttc aaaaacagac aagtcaggat tcatctctag tgtaatatcc aggtagatgt
    71101 tgctaccgta ggtgcgacct ctttgcgatt tgaccttgct gattttggga atttccatga
    71161 tagccttttg gtagtcctcg agcaggcggt cgtcaaagcc atctgaaaga ctaaaggaag
    71221 actcgatgaa gatatcatag gcagtcttca agataaagaa agtgatgatg atagcaacca
    71281 gtttatccac aatcggataa ttgaaactac tagctaggat ggcaatggcg gttccaagtg
    71341 aggtaacagc gtcagaaaga ttgtccttag cagctgcctt cagcgccttg gagttggatt
    71401 tcttactgag gcgagtattg tagagataga ccacaaacat aatcgctgca gaaatgattc
    71461 ctagagttgc accaagagga tcaatgaccg tttcttcccg actgagaatc ttttgaatgg
    71521 tatctcttag aacatcgaaa ccgacataga acatgatgat agaagtaatc aagcttgcca
    71581 aatcttcaat cttccaatga ccaaaacggt ggtcacggtc tgcaggctgg cgcgccatcc
    71641 gaatcccgat taagagggcc acatttccaa tgatgtccga tacgttatta aaaccatcgg
    71701 ccaccaaact ggatgaatga aggagatgac cagttgctaa tttggctgca gacaagatca
    71761 aataggtcga aatactgata atggctccac gctcagctaa cttgagattt gagatagatt
    71821 gcttcattca acttcctttc tagtcacata gcattctacc attatactgg ttttcaagga
    71881 aaaattcaaa taaggcagtt tttattcttt gaaaactccc caaaatttgg tataatggta
    71941 ctactcaagg gagtagctgg cagaaacctg tgatagtgtc gtcattccga attttatgct
    72001 gaaaagtatg ctttccggcc ctatcttaaa cagcgagact tgttatgatt aacaggtctc
    72061 tttttgtttg tcgtgaaaag atatgacaga aagagagtct gttttgcaca caatgtcaag
    72121 gaggagacac atgtcaaaag aacaaaaacg ccaagcgttt tatactcaga gccctgaaga
    72181 ggtcttgcag gctgtggatg cgaccgagca aggtttgtca tcaagtgagg cggaaaagcg
    72241 ccttgccgaa tttgggcaca atgaactcga agaaggcgag aaacgatcaa tcttggtcaa
    72301 attcatcgag caatttaagg atttgatgat tatcatccta gttgcggcag caatcttgtc
    72361 agtcgtgact tctggtgggg aagatatcgc agatgccatt atcatcctag ctgtggttat
    72421 catcaacgct gcctttggtg tttaccaaga aggaaaagct gaagaagcta tcgaagccct
    72481 caaatccatg tctagtccag ttgcccgcgt tcttcgtgat ggacacatgg cagagattga
    72541 ctctaaagaa ttggtaccag gcgatatcgt tgcccttgaa gcaggtgacg tggtaccagc
    72601 ggacctacgt ttgctagaag ccaactctct taaaattgaa gaagcagcct tgacaggtga
    72661 atctgtacca gtcgaaaaag acttgtcagt cgagtttgcg acagatgctg gtattggtga
    72721 ccgtgtcaac atggccttcc aaaactcaaa cgtaacctat ggtcgtggga tgggtgttgt
    72781 tgtcaataca ggtatgtaca ctgaagttgg tcatattgct ggtatgcttc aagatgcgga
    72841 tgagactgat acaccactca aacaaaactt gaacaacctt tctaaggtct tgacctatgc
    72901 tatcttggtc attgcccttg ttacttttgt agtgggtgtc ttcattcaag ggaaaaatcc
    72961 acttggtgag ttgttgactt ctgttgccct tgccgttgca gccattccag aaggacttcc
    73021 tgctatcgtt accatcgttc tttctcttgg tactcaagtt ttggccaaac gacattccat
    73081 cgttcgtaag ttgccagcag ttgaaacact tggttcaact gaaatcatcg cttctgataa
    73141 gactggtacg ctgactatga acaagatgac agtcgaaaaa gtcttctacg atgcggttct
    73201 acatgactca gctgatgata ttgaactagg tcttgaaatg ccactacttc gttcagttgt
    73261 cttggccaat gatacgaaaa tcgatgtgga aggtaacttg attggtgacc caaccgaaac
    73321 agcctttatc caatatgcct tggacaaggg ctatgatgtc aaaggtttct tagagaaata
    73381 tcctcgtgta gctgaattgc catttgactc tgaccgtaag ctcatgtcaa cagttcaccc
    73441 attgccagat ggtcgtttcc ttgtagcagt caagggtgcg ccagaccaac tcttaaaacg
    73501 ttgtcttctt cgtgataagg ctggggatat tgctccgatt gatgagaagg ttacaaatct
    73561 cattcataca aacaattctg aaatggctca tcaagccttg cgtgtccttg caggtgctta
    73621 taagattatc gatagtattc cagaaaatct cacttctgaa gagcttgaaa atgatttaat
    73681 ttttactggt ttgattggga tgattgaccc tgaacgtcct gaagccgctg aggctgttcg
    73741 tgtggctaag gaagcgggaa tccgtccaat tatgattaca ggtgaccacc aagacacagc
    73801 ggaagccatt gccaaacgtt tgggaatcat tgacgcaaac gatacagaag gtcacgtttt
    73861 aactggtgct gaactcaatg aactgtcaga tgaagaattt gaaaaagtcg ttggtcaata
    73921 ctctgtttat gcccgtgtgt ctccagaaca caaggttcgt atcgtcaagg cttggcaaaa
    73981 acaaggtaaa gtcgttgcca tgacaggtga cggtgtcaat gacgcgccag ctctgaaaac
    74041 agccgatatc ggtatcggta tgggaatcac tggtacagag gtttctaagg gggcttctga
    74101 tatgattctt gcagatgata actttgcgac tattatcgtc gcagtggaag aaggacgtaa
    74161 ggtcttctca aacattcaaa agactattca gtacctactt tctgctaata ctgctgaagt
    74221 attaaccatc ttcctatcaa ccttgtttgg ttgggatgtc ttacagccgg ttcatctttt
    74281 gtggattaac ttggtaacgg ataccttccc agctatcgct cttggtgttg aacctgcgga
    74341 acctggtgtc atgaatcata aaccacgtgg acgcaaggca agcttcttct caggtggtgt
    74401 tttgagttct atcatttatc aaggtgtact ccaagcagct cttgttatga gtgtttatgg
    74461 ccttgcgatt gcttacccag ttcatgtggg tgacaatcat gctattcatg cagatgccct
    74521 aacgatggcc tttgcaaccc ttggtttgat tcagctcttc catgcctaca atgtcaagtc
    74581 tgtctaccaa tccatcttga cagttggccc attcaagtct aagaccttta actggtccat
    74641 cttggtatcc tttatccttc tcatggcaac aatcgttgta gaaccgcttg aaggaatctt
    74701 ccacgtaacc aaactagact tgtcacaatg gggaattgtt atggctggaa gcttctcaat
    74761 gattatcatc gtcgaaatcg ttaagtttat tcaacgcaaa ctcggttttg acaagaatgc
    74821 gatttaaggg aggtttcgga tgtcagcata tcaattaccg accgtatggc aggatgaagc
    74881 tagtaatcaa ggagctttta cggggctaaa cagaccaaca gtaggtgccc gtttcgaaca
    74941 aaacttgcca aaaggagaac aagcttttca gctttattca ctgggaacac caaatggtgt
    75001 gaaggttact atcttattgg aagaattact agaagctggt tttaaggaag cggcttacga
    75061 cttgtataag attgctatca tggatgggga tcaattcgga tcagactttg tgaagctcaa
    75121 tccaaattcc aagattccag ccttattgga ccagtcaggt actgaagatg taagagtctt
    75181 tgagtctgct catattcttc tttaccttgc tgagaaattt ggagcctttt taccaagtaa
    75241 tcctgtggaa aaggtagaag ttttgaattg gctattctgg caagcaggtg cagcaccttt
    75301 tctaggtggg ggatttggac atttcttcaa ttatgctcct gaaaaattgg aatatcctat
    75361 taaccgtttt acaatggaag tgaaacgcca gttggattta ttggataagg aattggctca
    75421 gaaaccttat attgcaggca atgactatac gattgcagat attgctatct ggtcttggta
    75481 tggacagtta gttcaaggaa atctttacca aggttctgca aaattcttgg atgcctcaag
    75541 ttatcaaaat ctagtaaaat gggcagaaaa aattgccaat cgtccagctg ttaagcgtgg
    75601 cttggaagta acttatacag aaattaaata gcaaaaaagt catcttcgga tggctttttg
    75661 ctacagttga gggaaagggt ttgcagttat cggcttttgt gctataattg ctataatata
    75721 gtaaaaagca agaggagtgt gaggaagtgg aaaagaaaat tgtgaaggat atcttatttt
    75781 tatctcaagt gtctcagccg gcaagtcagg aggaccttta tcttgccaga gatttgcagg
    75841 atacactctt agcaaatcat gatacctgtg ttggtctagc tgccaatatg attggggtgc
    75901 agaagcgcgt gattatcttt aatcttggct tggttcccgt ggtcatgttt aacccagtgc
    75961 ttctgtcctt tgaaggatcc tatgaggcag aagaaggctg tttgtccttg gtaggcgtga
    76021 gatcaactaa gcgttatgaa accataaggg ttgcctatcg tgacagcaag tggcaggaac
    76081 agaccattac cttgacaggc ttcccagctc agatttgcca gcatgagctg gatcacttgg
    76141 aaggacgaat catttaggag gaaagcaaat gaaacgaata gtctttgaac ttatttttat
    76201 cgcaacgacc tggtatatct ttttaccgcc ccttaacctg accagctggg aatttctctt
    76261 cttcctctgt gggcatttgt tagttgtggc aatcttattt ggctttggca aggggataaa
    76321 ccttgtcaaa acggttcatg tgcgccacgg taaggcggaa gctgccttaa atcttgaggg
    76381 tttcaaaatc aatcggttag ggaaaattct gttagcttcg attggaggaa ttcttctctt
    76441 ggcagctttg gtttccttgg taacttccag catgtttcag gctaaaaatt atgccaatgt
    76501 agtcacggtt acggaaaaag actttactga atttcctagg agtgacacca gtaaggttcc
    76561 tatcctagat agaagtactg ctgaaaaaat tggagaccgc tacttgggtt ccctaaccga
    76621 taaggtgtcg caatacgtag cggcagatac ctatacccaa ttgacaattg atgggaaacc
    76681 ttatcgggtc acaccactag aatatgcaga ccctatcaaa tggtttaaca atcaagccaa
    76741 gggaatcggt gagtatatca aggtggacat ggtaactgga aatgcggatt tggtggactt
    76801 gaagacacca atcaagtatt cagactcgga gtattttaac cgtgatgtca aacgtcacct
    76861 gcgcttgaag tacccgacca aaatctttaa aactccatct tttgaggtgg acgatgaggg
    76921 caatcctttc tatgtagcaa cggtttacca aaagcaattt ggacttgctg ttcctcgtcc
    76981 tgcttcagtc attatcttgg atgctacaaa tggagaaacc aaggaataca gcttatcaga
    77041 tgttccagaa tgggtggaca ggatctatcc agcagaggaa accattgagc aaatcaacta
    77101 caacggcaag tacaaggacg gtttcttgaa tgccatgatt tccaagaaaa acgtgaccca
    77161 gactaccaag ggctataatt acttgtctat cggtaatgac atctatctct acacaggtgt
    77221 gacgtcggct aatgcggatg agagtaatct tggtttcatc cttgaaaata tgcgaacagg
    77281 agaaatcact aagtatagct tggcttctgc gacagaagaa tcagcccgtg aatcagcaga
    77341 aggtgctgtt caggagaaat cctacaaagc aaccttccca atcctcatca acctcaatga
    77401 caagcctctc tacatcatgg gcttgaagga caatgctggc ttggtcaaag agtacgccct
    77461 ggtagacgca gtcgagtacc aaaatgttat cgttgctact acagtggaag agatgctcag
    77521 caagtatgcc aataaaaacg accttgaaat tgacaatgca acgacagaaa gcatcaatgg
    77581 agtagtagca gacctcaaat cagctgttat caagggagac actgtctact tctttaaagt
    77641 tgatggcaag atatacaagg tcaaggcttc agtatccgat gaccttcctt accttgaaaa
    77701 tggtaaaacc ttcgaaggtc aagtaggaaa agacaattat ctcaagacct ttaagctacg
    77761 gtaaaaatag gtttttttca gaaagtatat gttataataa ggtaaattaa gcctaatgga
    77821 ggacaaagaa atgggttttt atttgatggt tgcttcaatg ctactcggac tgctcgcttt
    77881 gaagataggt ttttctcagt tcaaggagaa aaaagataaa ttcttatcta tcttaacaag
    77941 tttagctggc acagcccttg ttctcgtggc tgtctggtta ggatggccca aataaataga
    78001 aaaacctcgg tcaaaccgag gtttttcaaa tgaatttctt ggttgtggct aaaaaatatg
    78061 ctacactatc aatatgaaaa ttttaatccc aacagcaaaa gaaatgaaca cagacttgcc
    78121 aagtatcgag gcaatttctt taaaaccaga aagtcaggcc gtgcttgatg ccttggctct
    78181 ctattctgcc agtcaattgg agagtttcta caaggtatca gctgagaaag cggcggaaga
    78241 atttcaaaat atccaagctt tgaaaaggca aactactcaa cactatccag ccttgaaact
    78301 ttttgatggg cttatgtacc gcaacattaa gagagataag ctgaccgagg cggaacaaga
    78361 ttatcttgaa aatcatgtct tcattacctc ggctttgtac ggtgttgttc cagccttgtc
    78421 acccatggct cctcaccgtt tggatttttt gatgaaatta aaagttgctg gtaagacttt
    78481 gaagagccat tggaaggcag tctatgatga agctctgaag aaggaagaag tgattttctc
    78541 tctcttgtca tcagagtttg agactgtatt ttctaaggaa atcagagcaa agatggtgac
    78601 cttcaaattc atggaggata gaggcggtca gctgaagatt cactcaacta tctccaagaa
    78661 agcgcgcggg gcctttctaa cagctttaat agaaaatcaa gtacaaactg tgggggaagc
    78721 acgtcgcttg aactttgctg gatttgttta ccgagaagat ttgtcacaac cacagggatt
    78781 ggtttttgta aaggaagtat agaaatcaga tattttgtat ttggcaatat gtatcatcag
    78841 ctattttagt ttataaatga gaaaaaagcg aacaagctag tcttctgagc gtcagaaaag
    78901 tagtcttgtt cgcttttatt atgttatact cttcgaaaat ctcttcaagc catgtcagtg
    78961 tcgccttacc gtatatatgt tactgacttc gtcagttcta tctacaacct caaagcagtg
    79021 ctttgagtag cttgcggcta gcttcctagt ttgctttttg atttttattg agtgttagtt
    79081 acttgatagc ttcgaccaag tcttgagctt gtttttcaag tgagtttagg actgtttctt
    79141 caagaaccaa ttttccgtct gcccaggcag agtcattaac acgtgcagca gtgaaatcac
    79201 caacgccttg tgtacggata aatggcaaga ggtctttgta gatagcgaaa agttgatcgt
    79261 gccctgcatt ggctacagat gatactgtga caaacttgtc ttgaagggca gaaacgccac
    79321 gtgtatcaga caagtcaagg gcacgagata gccagtcaag caagtttttc actgtaccag
    79381 ggatagagaa gttgtagact ggagagaaaa tccagatagc atccgcaacg agaactgctt
    79441 cacgagcagc agctacagct ggatgtgttg gaacttccaa atcttggcta aagagaggaa
    79501 gggctgaata atcaaggtag ctaacttccg ctttaccagc aagtgctttc tcagcttcga
    79561 gcgccatttg gtggttgaaa gaaccttgac gtagtgatcc gacgataaat aatacttttt
    79621 tagacatgat gtgtctcctt tttcttaaaa tttcgagaat tctctcgttg ttataaatta
    79681 tattacaaca aaacaaaaga acttgcaaga aatttgctca gagtttattt gaaaattgta
    79741 aaatttcgga aactctagtc atttgtgact gaaaaagtaa gaactgtttc actttcaaaa
    79801 tcggttttgt ttgaagggag aaatagaatt ggttgctcaa agcacagctt tgaggttgca
    79861 gataaagctg acgtggtttg aagagatttt cgaagagtat tcttatattc acttgtaata
    79921 aagcttaata gatggtaaaa tagacgagtg aaaaaaagac aaaacaaagc aaaaaataat
    79981 ctactgtggc agtatggtct agggatgacg attttgtttg tggttatcag tggttccttt
    80041 ctgtatatgg tttctcttag catgaaaccc tatcaaacag ctaaaagtga aggagaaaaa
    80101 ttagctcagc agtatgcagg attagagcag gctgttcagg ttgatttata caatggcttg
    80161 gaatcttatt acagcgttct tggtcgtaat aaacagcaag aagcgcttgc tgtcctgatt
    80221 ggtaaagatg accataagat ttacgtttat cagctaaatc agggtgtttc acaagaaaaa
    80281 gcagaaacgg tttctaagga aaagggagct ggcgagattg acaagataac ctttggccgt
    80341 tatcaagata agccaatctg ggaagttaag tcaggatctg atttttatct agtagatttt
    80401 gaaacaggag cattggtcaa caaggagggc ctatgaaatt atccaaccgt gttttagaaa
    80461 tggaagaaag tgtgactttg gctgctggag cccgtgccaa agcactgaag gctgagggta
    80521 gagatatttt gtctctaacc ttgggtgagc cagatttcac tactcccaaa aatatccaag
    80581 atgccgccat tgcatcgatt cgagatgggc gtgcttcttt ttatacagta acctcaggtc
    80641 tgccagagct taaggcggcg gtcaatagct actttgagcg cttttacggc tattctgtag
    80701 cgtcaaatca agtgacagtc gctgcgggag ccaaatattc tctctatacc ttctttatgg
    80761 ctgtggtcaa tccaggtgat gaagtcatca tcccaacccc atactgggtt agctatggag
    80821 atcaggtcaa gatggcagaa ggcgttcccg tctttgtttc tgctaaggaa gacaatcact
    80881 ttaaggtgac cgtggagcag ttagaagcag ctcggactga caagaccaag gttttggtgc
    80941 tgaattcgcc atctaatccc acaggtatga tttacacccg tgaggaactc ttggcaattg
    81001 gaaactgggc tgtagaaaat gatattctca tcctagcaga cgatatctat ggccgcttgg
    81061 tttataatgg tcatgagttc acaccgattt ctagcctatc ggaagcgatt cgcaagcaaa
    81121 cagtggtcat caatggtgtg tctaaaacct atgccatgac tggttggcgg attggttatg
    81181 ccgttggaga agcagacatt atcgctgcca tgtccaagat tgcaggtcaa acaacttcga
    81241 atccgtcagc agtagcccaa tatgcagcag ttgaggctct atcaggtgag caagatactg
    81301 tagaaagcat gcgtcaggcc tttgaggaac gtcttaatac tatctatccc ctccttgcag
    81361 aggtgccagg atttgaagtg gtcaaaccgc aaggggcctt ctatctcttc ccaaatgtca
    81421 aaaaggccat ggagatgaaa ggctacacgg atgtgataga ctttacaact gctatcttag
    81481 aagaagccga agtggccttg gtaacaggag ctggatttgg agcaccagaa aatgtccgcc
    81541 tcagctatgc gacagaccta gacacgctta aagaagcagt cgaacgcttg aaagcattta
    81601 tgggtagtga gaatgattga tcattttgag attaaggtaa aggatttaca aatttcagaa
    81661 ggattttata ggagttttct cgctcctttg gactataaaa tggcttttaa gactagttct
    81721 ctaattagtt ttctatcccc gaacagccct catcctggtg gtgatttttg gctgactcag
    81781 ggaacacaag atcctgttca ttttgctttt ttagcagaaa ataaggaaga agttcaggct
    81841 tgttatgagg ctggcataga ggcaggtggg cgagacaatg gggttcctgg ttatcgaagt
    81901 gagcatccga tttactatgc tgcttttatg attgacctgg atgggaacaa tatagaggtg
    81961 gtttgccata aagaataaaa aagaaaagga aaaataatga caaaacgtgt aacgattatt
    82021 gacgtaaaag actatgttgg tcaggaagtg acgattggcg cttgggttgc caacaaatca
    82081 ggaaaaggaa aaatcgcttt cttacaattg cgtgatggaa cagccttctt tcaaggtgtg
    82141 gcttttaaac caaactttgt cgaaaaattt ggtgaagaag tgggacttga gaagtttgat
    82201 gttatcaaac gcttgagcca agaaacgtct gtttatgtga caggtattgt caaagaggac
    82261 gaacgttcta aatttggcta tgagttggac atcacagaca tcgaagtgat cggtgaatct
    82321 caagactacc caatcacacc aaaagaacac ggaacagact ttttgatgga taaccgtcac
    82381 ttgtggctac gctctcgtaa gcaagtagct gtgttgcaaa tccgtaacgc tattatctat
    82441 gcaacttatg agttctttga caagaacggt tttatgaagt ttgacagccc aattctttca
    82501 ggaaatgcgg cagaagattc tacagaactc tttgaaactg actacttcgg aacgccagcc
    82561 tacttgagcc aatcaggtca gctttaccta gaagcagggg ctatggctct tggtcgtgtc
    82621 tttgactttg gtccagtttt ccgtgctgaa aaatcaaaaa cacgccgtca cttgactgag
    82681 ttctggatga tggatgctga gtactcatac ttgacacatg atgagtcgct tgacttgcaa
    82741 gaagcttatg tgaaagctct tctacaaggt gttcttgacc gcgcgcctca agccttggaa
    82801 accttggaac gtgatacaga actcttgaaa cgctacattg cagagccatt caaacgtatc
    82861 acttacgatc aagccattga cctcttgcaa gagcatgaaa atgatgaaga tgctgactac
    82921 gagcatcttg agcatggtga tgactttggg tcaccacacg aaacttggat ttcaaaccac
    82981 tttggtgtgc caacatttgt catgaactat ccagcagcca tcaaggcctt ctacatgaaa
    83041 ccagttcctg gaaatccaga gcgcgtgctt tgtgcagact tgcttgctcc agaaggctat
    83101 ggagaaatta tcggtgggtc tatgcgtgag gaagattacg atgcccttgt cgctaagatg
    83161 gatgaacttg gcatggatcg tacagaatat gaattctacc ttgaccttcg taaatacggt
    83221 acagttccac acggaggatt tggtatcggt atcgaacgta tggtaacctt cgcagcagga
    83281 acaaaacata tccgtgaagc tattccattc ccacgtatgt tgcaccgtat caaaccataa
    83341 gaacaggaaa acgcccatgt ggcgtttttt cgaattctag tgtttttact tgcgtaaaaa
    83401 aattttttct agtataatag tttattgtga gcgaacctca cttacccctt gcaaagtctt
    83461 ggggtcatta gaccaaaagg aggaacatat caatggctaa atacgaaatt ctttatatca
    83521 ttcgtccaaa cattgaagaa gaagctaaaa acgctttggt agcacgtttt gactctattt
    83581 tgactgacaa cggtgcaact gttgttgaat caaaaacttg ggaaaaacgt cgtcttgcat
    83641 acgaaatcca agatttccgt gaaggacttt accacatcgt taacgttgaa gcaaatgacg
    83701 atgcagctct taaagagttt gaccgtcttt caaaaatcaa cgctgacatt cttcgtcaca
    83761 tgatcgtcaa aattgacgcg taagaaggta aactatgatt aacaatgttg tacttgtagg
    83821 gcgtatgaca cgtgacgctg agttgcgtta taccccatca aatgtagcag ttgcgacttt
    83881 tactcttgca gtaaaccgta catttaagag tcaaaatggt gaacgtgagg ctgattttat
    83941 aaatgtcgtt atgtggcgcc aacaggctga aaatcttgct aactgggcta aaaaaggctc
    84001 acttatcggg gtgacaggtc gtatccagac tcgtagttac gataaccagc aaggacaacg
    84061 tgtctacgtg acagaggtcg tggctgagaa tttccaaatg ttggaaagcc gtagtgtgcg
    84121 tgagggtcac acaggtggag cttactctgc accaactgca aactattcag cacctacaaa
    84181 ttcagtacca gacttttcac gtaatgaaaa tccatttgga gcaacaaacc cattggatat
    84241 ttcagatgat gatttaccat tctaatggac aactaaactt ataaaggaga aaaaacatgg
    84301 ctcaacaacg tcgtggcgga ttcaaacgcc gtaaaaaagt tgattacatc gcagcaaaca
    84361 aaattgaata tgttgattac aaagatactg agcttcttag ccgtttcgtt tcagaacgtg
    84421 ggaaaatcct tcctcgtcgt gtaacaggaa cttcagctaa aaaccaacgt aaagtaacaa
    84481 cagctatcaa acgcgctcgc gtaatggctt tgatgccttt cgtaaacgaa gattaaagaa
    84541 aagacccggt tgggtctctt tttcatgagc ttgaaaacaa gaaagcgaat taagacccca
    84601 atgggtcttt tttcacaaac tttgaaatga gagagcgagt taggctcagt ggctcccttt
    84661 ttacgagttt gaaaacaaga gaacacatta aaagcacgat ggagcttttt cataaatatg
    84721 tcttttttct catccaagaa attaaaataa aaatgataaa ctttttgaaa ataggggatt
    84781 tgcctacttt atgatataat ggaaagagtt agattgaaag aggtagtgag atggacgcga
    84841 aattaagata caaggcaaag aagatcaaga ttgtcttttt tgatattgat gatacattgc
    84901 ggaattcaaa gacagggttt attccaacta caattcccac tgtttttaaa cagttgcgtg
    84961 aaaaaggaat tttaacagga atcgcctctg gacgtggcat ttttggtgtt gttccagaga
    85021 ttcgtgatct caagcctgac ttttttgtaa ctttgaatgg ggcttatatc gaagataaaa
    85081 aaggtcaggt catttatcag catcagattg aaaagtcaga tgttgaggaa tatatctctt
    85141 gggctaagca agaaggaatt gagtatggct tggttgggag tcatgatgcc aagttgtcga
    85201 ctcgcaccga tatgatgagt gaagctatca atccaattta tcccgactta gatgtagatc
    85261 ccgatttcca tgaaaaagaa gatatctatc agatgtggac ttttgaagat aagggagatg
    85321 acttgcactt gcctgacagt ctctcagaca aacttcgcat ggttcgttgg catcaacatt
    85381 cgtctgatat tgtgccgatt tcaggctcca aagcgacggg ggtggaaaag gttgtggaac
    85441 accttggctt gaaaccagag aaggtcatgg tttttggaga tggtctcaat gacttggaac
    85501 tctttgatta tgctggaatc agcgttgcca tgggaatttc tcatgataaa atcaaagaaa
    85561 aagcagatta tattacaaaa acattagaag aagatggcat ttttgctgcc ttagaagtat
    85621 ttggtatggt agaaaaagaa ttgcattttc cacaagtaga cattgaaaca gtagaaggtc
    85681 ctcttgcgac tattaagacc aatcacggag acttacgtat caagcttttc cctgaacatg
    85741 ctcctaaaac agtggctaac tttgtatctc tttcaaaaga tggctactat gatggtgtca
    85801 ttttccaccg tattatcaag gactttatga tccaaggtgg agacccaact ggaactggta
    85861 tgggtggcga gtcaatctac ggcgaatctt ttgaggatga attctcagaa gagctttaca
    85921 atatccgtgg tgctctttcc atggcaaatg ctggtccaaa taccaacggc agccagttct
    85981 ttatcgtgca aaaccaacac ctaccttatt cgaagaaaga aattactcgt ggtggttggc
    86041 cagaaccgat tgcagaaatc tatgccaatc aaggtgggac acctcaccta gaccgccgtc
    86101 acacggtttt tggtcagtta gctgatgaag catcttacgc tgtcttggat gccattgctg
    86161 ctgttgagac aggagctatg gacaagccag ttgaagatgt tgtaattgaa actattgaaa
    86221 tcgaggacta agatgaaaat cggtgataag ctaaaggggc gtattacagg gattcagccc
    86281 tacggtgcct ttgttgagtt agagacgggt gatacggggc tgattcacat ttcagaaatt
    86341 cggacaggat ttattgaaaa tattcatgaa accttgaaag tcgatgaaga ggttcaagtt
    86401 caggtagtgg atttagatga atttacgggg aaagccagtc tttctatccg cactttggag
    86461 gaagaaaagt accagtttcc tagacggaga cgcttttcaa gcgaccgctt taactacggt
    86521 tttgctccct ttcgacgaat gttaccaatc tggactggag aagccctgca ccatttaaaa
    86581 aagaagaagt agtttagagt tttatatttt ttgtaatgtt aatataaatt tgctataata
    86641 ggaccatgga agaagaacaa ttattaaaat caggagagcg cattaatcag ctcttttcga
    86701 cagatatcaa aatcattcaa aatagagagg tttttagcta ttcggtggat agtgttctct
    86761 tatcacgatt tccacgtttt cctaagaagg ggctgattgt ggatttctgt gctgggaatg
    86821 gagcagtggg gctttttgct agcactcgta ctcaagcaca gatattgtct gttgagattc
    86881 aggagcgttt ggcggatatg gctgaacgct ctgtccgttt gaatggatta gaggagcaaa
    86941 tgcaggtcat ctgcgatgat ttgaaaaata tgcctgctca cattcagggg agtaaggtgg
    87001 atatgatttt gtgtaatcca ccctatttca aggtggttcc ttattctaat ctgaacgaga
    87061 gtgaacatta cctcttggct cgacacgaaa tcacgactaa tttggaagaa atctgtcgta
    87121 gtgcccagag tattctcaag tctaatgggc gtttggccat ggttcatcgt cctgatcgac
    87181 ttctggatat tttggatacg ttaaaacgac ataatctagc ccctaagcgc ctgcagtttg
    87241 tttatccaaa aagagaaaag gaagccaata tgcttttgat tgaggcgatc aaggatggct
    87301 caacaagtgg ctttaaggtc ttacctcctt tgattgtcca caatgatgat ggctcttata
    87361 cgcccgaaat cgaagagatt tattatggat cataaggctt atatgtacgt tctggagtgt
    87421 cgtgatggat cctactatat aggctatacg actgatgtga gaagacgcct cgctatccac
    87481 aatagtggga agggagccaa atatacacga gcacgcttgc cagtcaaact tatctatgct
    87541 caaggttttg ccagtaagga agaagccatg tcggctgaag ctcttctcaa gcgtaagaag
    87601 aggccacaga aggaagaatt tttatctgaa aatcaagata gaaatttact cagttatttt
    87661 gaagaatcgt ggggagtcct ttgactcctc ttacctttgt caaaaagcga aaaaaatgct
    87721 acaataaaga aaataaacaa aatgaggtat tatcatgtcc aagattctag tatttggtca
    87781 ccaaaatcca gactcagatg ccatcggatc atctgtagct tttgcctacc ttgcaaaaga
    87841 agcttacggt ttggatacgg aagctgttgc ccttggaact ccaaatgaag aaacagcctt
    87901 tgtcttgaac tattttggtg tagaagcacc acgtgttatc acttctgcca aagcagaggg
    87961 agcagagcaa gttatcttga ctgaccacaa tgaattccaa caatctgtat cagatatcgc
    88021 tgaagtagaa gtttacggtg ttgtagacca ccaccgtgtg gctaactttg aaactgcaag
    88081 cccactttac atgcgtttgg agccagttgg atcagcgtct tcaatcgttt accgtatgtt
    88141 caaagaacat ggtgtagctg tgcctaaaga gattgcaggt ttgatgcttt caggtttgat
    88201 ttcagatacc cttcttttga aatcaccaac aacacaccca acagataaaa tcattgctcc
    88261 tgaattggct gaattggctg gtgtgaactt ggaagaatat ggtttggcaa tgttgaaagc
    88321 tggtaccaac ttggctagca aatctgctga agaattgatt gacatcgatg ctaagacttt
    88381 tgaactcaac ggaaataatg tccgtgttgc ccaagtgaac acagttgaca tcgctgaagt
    88441 tttggaacgc caagcagaaa ttgaagctgc aatgcaagct gccaacgaat caaacggcta
    88501 ctctgacttt gtcttgatga ttacagatat cgtcaactca aactcagaaa tcttggctct
    88561 tggtgccaat atggacaagg tcgaagcggc tttcaacttc aaacttgaaa acaatcatgc
    88621 cttccttgct ggtgccgttt cacgtaagaa acaagtggta cctcaattaa ctgaaagctt
    88681 taatgcgtaa gattttgggt gtcagctcaa aatcggaaag tctagtttgc cttatatcga
    88741 aaggagtttc ggctcctttt tttctaggag tgaagtatgt tagaaaatgg cgatttgatt
    88801 tttgtgagag atgggtcaga catgggacag gccatccaga cttccacagg taactatagc
    88861 catgttgcca tttatttgga tgggatgatt tatcatgcta gtggacaggc tggtgttgtc
    88921 tgtcaagaac cggcagactt ctttgagtcc aatcatttgt acgacctcta tgtttatcca
    88981 gaaatggata tccagtcggt gaaggaaaga gcttgcaaac atcttggagc accctacaat
    89041 gcttctttct atccagatgc acctggtttc tactgctccc agtatatagc agaaatccta
    89101 cctatttttg aaactattcc tatgaaattt ggagatgggg agcaggagat tagtgatttt
    89161 tggagggagt attacataga actaggtctg cctgttcctc tgaaccaagc tggtaccaat
    89221 cctagtcagt tggcagcatc gcctctgtta caatgtaaag aaaggaatct tcatgattca
    89281 gatttttaat ccatctcgtt tgacgagaca gccatttttg gagaattgat ccgctatctg
    89341 gaccagtatg aggatgtgat tctacgggaa attaaggctc aatttccaga tgttgcagtt
    89401 gataaactca tggaagagta tataaaggca ggcttgattc tacgtgaaaa taagcgctat
    89461 tacctcaatt ttcctacgct tgaatcactt gatagtcttg aactggatca agagattttt
    89521 gtcagagaag ctagtccggt ctatcaagcc ttgttggagc agagttttga gacggaattg
    89581 cgcaatcaaa tcaatgcagc tattttagtt gaaaagacgg actttgcgcg cattaaaatg
    89641 accctgtcca attattttta caaggtcaaa cagcagtatc ctttgacaga aaaacagcag
    89701 gagctctatg acattttagg agatgttaat cctgagtatg cactcaagta tatgacggct
    89761 tttttgttga aatttctcaa aaaagaccag cttatgcaga aatgccgtga tatctttgtg
    89821 gacagtttgg ttgtcttagg ctatattgtg caaaatgaag atggaaagta tgagttggct
    89881 atcgattttg ataaggagag attaactttc tacttagcgt gatttcttgt ttctgagtac
    89941 attgtttgac tttccttagt attcggtata aactatatgt aaccggtaac acatatcgga
    90001 ataaactaaa ggagacaatc atatgtcact tgaaaacaaa ttggaacaag caacaggcgc
    90061 tgtcaaagaa ggttttggta aagttactgg agacagcaag acagaacttg aaggagctgt
    90121 tgaaaaaaca gttgctaagg caaaagacgt tgtagaagac gcaaaaggtg ctgtagaagg
    90181 tgccgttgaa ggtttgaaaa acgtttttac taaagaatag gaaaaaatca agggtttcat
    90241 tttcccttga ttttttctat tcttataaat aattttctgc gacggctgta tctcctgggt
    90301 aggattcttt cttgccctgg atgatttggt aacaatcggc tcccttaccc gcaataataa
    90361 ctgcatctaa ttcgtgattt gtgatagcca ttgccgcctt gatggcttct tggcgatccg
    90421 caatcttctc aacaggatga ttgatgtagc tactaatttc atctgcaatg gccattgggt
    90481 cttcatagtt agggtcatca gcagtcagaa agacttgaat ctcagggtgt tgattgagga
    90541 ggaggccaaa gtccttacga cgactttctc ccttgtttcc tgttgatccc agaaccagag
    90601 caatctttcc ggtttgatga gtttcaacca cattgatgag ttttttcaga ctatccccat
    90661 tgtgggcata gtcgatgaag accttggctc catttttctg agtgaggact tccatacgac
    90721 caggaacgcg ggttgcagcg atgccttttt tgatgtcctc aagacttgct ccgagacgga
    90781 gacaagcaag tccagcagca actgcatttt cttggttgaa gttgccaatg agttggatat
    90841 cataatctcc agcgagttta cccgtagctg aaaagctaaa ggctttggaa ttctcgattt
    90901 ggttatcaaa ttggctacca tagaaatcat ggtcttgatc ttcaacctgt tctttcaaga
    90961 ctgagaagtg gtccatgtca ctgttaatga tgactgctcg gctattttcc atcaagagac
    91021 gcttgtggta gaaatagtct tcaaagctag ggtgttcaat cgggccgata tggtctgggc
    91081 tgatattgag gaaaactccc acatcaaagg ttagaccata gacacgtctg accagatagg
    91141 attgactgga gacttccatg atgaggtggg tacggtcatt ttgcacagcc tgattcatca
    91201 tgtcaaagag gtcaatactc tcaggggttg tcaacgctga cttaaagaaa gtctcgccat
    91261 caagagttgt gttcatggtc gacaacatag caggtcgatg cccttgagat aagatgttat
    91321 aggcgaaata ggctgctgtt gtcttaccct tagtaccagt aaaggcaagg agtttgagtt
    91381 tttcctgtgg attaccatag aactccatgg caatcaaact catggctttc tttatatcgt
    91441 tcacaatgat gacagggata ccgacttcgt agtccttttc agctacatac caagctaagc
    91501 cttgtgttat agcagaaaga aggtattctt ttttaaaggc agcgcctttt gcaaaaaaaa
    91561 gagtgtcttc tgttactttt cggctatcgt agctgatgct atcaaaaaca actttgctgt
    91621 agttgtagtg gtaatgacct tggtcaataa tttcgcgaaa aaggccatct ttctttaaaa
    91681 tatctaatac agtttcaatt tttatcatac tttctattgt aaaccgaaag tcgcaaattt
    91741 acaagtaaca aggaaaagtt tataatggaa gataaggagt ttttcctagt tatcaaaatt
    91801 gaatgaggaa tctatgtcgc acgaaaacaa tcaccagcag gcccagatgt tacgggggac
    91861 tgcttggcta acggctagta actttatcag tcgcctactc ggggctgttt acattatccc
    91921 ttggtacatc tggatggggg cttatgcagc taaggcaaat ggtctcttta ccatgggtta
    91981 caatatctat gcttggttct tgttggtttc aacagcgggg attccagttg cggtggccaa
    92041 gcaagttgcc aagtataata ccatgcgaga agaagagcat agctttgccc tgattcggag
    92101 cttcttaggc tttatgacag gactaggcct ggtttttgct ttagtcttgt atgtctttgc
    92161 tccttggcta gcagacttgt ctggcgtggg caaagacttg atcccaatca tgcaaagctt
    92221 ggcttgggga gtcttgattt tcccgtctat gagtgttatc cgaggatttt tccaagggat
    92281 gaataacctc aaaccttatg ccatgagcca aattgctgag caggtcattc gtgttatctg
    92341 gatgctccta gcaaccttta tcattatgaa gctcggttca ggagattatc tagcagccgt
    92401 tacccaatca acctttgctg cctttgtcgg tatggtagcc agttttgcag tcttgattta
    92461 tttccttgcc caagaaggtt cactcaaaag agtctttgaa acaggagata agattaacag
    92521 taagcgtctc ttggttgata ccattaagga agccattcct tttatcctga cagggtctgc
    92581 catccagctc ttccagattt tggatcagct gacctttatc aatagtatga gctggtttac
    92641 caactacagc aatgaggact tggttgtcat gttttcttat ttctcagcca atcctaataa
    92701 aatcacgatg attttgattt ctgtaggggt ttcgattggg agtgttggtt tgccactttt
    92761 gacggaaaac tatgtcaagg gggacttgaa agcagcttct cgtctggttc aggacagtct
    92821 caccctactc tttatgttct tgctaccagc aacggttgga gtggttatgg taggagaacc
    92881 tctatataca gtcttctatg gtaagccaga tagtttggct ctgggcttat ttgtctttgc
    92941 agttttgcag tctattattt taggcttgta catggtcttg tctccaatgc ttcaggccat
    93001 gttccgcaac cgcaaggccg ttctctattt tatctatggt tctattgcca agctagtctt
    93061 gcaactacct accatcgccc tcttccacag ttatggtcct ttgatttcaa caaccattgc
    93121 tctcatcatt cctaacgtct tgatgtatcg ggatatttgt aaagtaactg gtgtcaagcg
    93181 caaggtgatt ttgaagcgaa ccattttaat cagtttgctg accctagtca tgtttctgtt
    93241 aataggaacc atccagtggc tgttaggatt tttcttccaa ccaagtggac gtttgtggag
    93301 cttcttttat gtagctcttg tcggtgccat ggggggtgga ctttatatgg ttatgagtct
    93361 gcgtacctat ttattagata aggtaatagg aaaagcccaa gcagatcgcc tgcgagcaaa
    93421 atttaagctt tcgtaaggta gcatttataa ataaaaggaa taagttgaac aatcttttag
    93481 gagaacttga aacttattcc ttttttagcg tcaaaatagc taaaattatc tttcaaaaat
    93541 agaaaatgag gtttttttga ttttttggaa aaaacaattg actaaacaaa ccaagagttg
    93601 tataataaaa caaatattta aagcaggagg ttctggaaat gaaaaagtct aagagcaaat
    93661 atctaacctt ggcaggtctt gtcctgggta caggagtttt attgagcgcg tgtggaaatt
    93721 ctagcactgc atcaaaaacc tataactatg tttattcaag tgatccatct agtttgaact
    93781 atctagcaga aaaccgcgca gcaacatccg atattgttgc aaatttggta gacgggttat
    93841 tagaaaatga ccaatatggg aatattattc catcattagc agaggattgg actgtttctc
    93901 aggacggttt gacctatacc tacaaacttc gtaaggatgc caagtggttt acttctgagg
    93961 gagaagaata tgcgcctgta actgcccagg attttgtgac aggtttgcaa tatgcagctg
    94021 ataaaaaatc agaagctttg tatctagtgc aggactctgt tgctggtttg gatgactata
    94081 tcactggtaa aacaagcgac ttttcaactg tcggtgtcaa ggcacttgat gaccaaacgg
    94141 ttcaatatac tttggttaaa ccagaacttt actggaattc aaaaacactt gcaacgatac
    94201 tttttcctgt taatgcagat ttcctgaaat caaaagggga tgattttggg aaggcggatc
    94261 catctagtat tttgtacaat ggacctttct tgatgaaagc acttgtctca aaatctgcta
    94321 ttgaatataa gaaaaaccct aattactggg atgctaagaa tgtctttgta gacgatgtga
    94381 aattgaccta ctatgatggt agcgaccaag aatcactgga acgtaatttt acagctggtg
    94441 cttatactac ggctcgtctt tttcctaaca gctccagcta tgaagggatt aaagaaaaat
    94501 acaaaaacaa tatcatctat agtatgcaaa attcaacttc atatttcttt aattttaacc
    94561 tagataggaa gtcttacaat tatacttcta aaacaagtga cattgaaaag aaatcgaccc
    94621 aggaagcagt tctcaataaa aacttccgtc aggctatcaa ttttgctttt gacagaacat
    94681 cttatggggc tcagtctgaa gggaaagaag gtgcaacaaa gattttgcgt aacctagtgg
    94741 ttcctccaaa ctttgtcagt atcaagggaa aagactttgg tgaagttgta gcctctaaga
    94801 tggtcaacta tggtaaggaa tggcaaggta tcaactttgc ggatggtcaa gacccttact
    94861 acaatcctga gaaagccaag gctaagtttg cggaagctaa gaaagaactc gaagcaaagg
    94921 gtgttcaatt cccaatccac ttggataaga ctgtggaagt aacagataaa gtaggcatac
    94981 aaggagttag ttctatcaaa caatcaattg aatctgtttt aggttctgat aatgtagtga
    95041 ttgacattca gcaattaaca tcagatgagt ttgacagttc aggctacttt gctcaaacag
    95101 ctgctcagaa agactatgat ttatatcatg gcggttgggg acctgattat caagacccgt
    95161 caacctatct cgatattttt aatactaata gtggaggagt tctacaaaat cttggactag
    95221 agcctggaga agccaatgac aaggctaagg cagttggact ggatgtctat actcaaatgt
    95281 tggaagaagc taataaagag caagatccag ccaaacgtta tgagaaatat gctgatattc
    95341 aagcttggtt gattgatagt tctttagttc ttccaagtgt ttcgcgtggg ggaacaccat
    95401 cattgagaag aaccgtacca tttgctgctg cctatggttt aaccggtaca aaaggggttg
    95461 aatcatataa atacctcaaa gtacaagata agattgtcac aacagacgaa tatgcaaaag
    95521 ccagagaaaa atggttgaaa gaaaaagaag aatccaataa aaaagcccaa gaagaattgg
    95581 caaaacatgt caaataaaat ctaattgtag ataagtttgt gtaagaagtc tttgtgctta
    95641 aaaacgcata atatcaggta tataaaatcc tagatgttat gcgttttgtt ataggaagat
    95701 ttattagatt ttatcctcaa attgagttat tcccgacttt atccgttgaa atattataag
    95761 gagtatttat gaaattaaaa ttattaagag tagatactaa ggtgattatg gggagtttct
    95821 tacttgttct gtctagtcta cttgctttgt tgcttcccct tatcttaaag ggtttaatag
    95881 atgggagttc tattgaaaat ataggttcca aagtatttca atcgtttttg atttttattg
    95941 gtcaagcctt gttttcttct attggttact atctgtttag tcaatcgggt gaaaaaaaga
    96001 tagcaaaaat caggaaaaaa gtgatagagg ggttgattta tgcagagaaa tccttctttg
    96061 ataagagcca aagtggggag ttgacttctg ccattgtcaa tgacatgagt gtcattcgtg
    96121 agtttttaat tacgactttc ccaaatatta ttctgagttt agttatggta cttggttcca
    96181 ttgtagtctt atttagtctt gattggaatc tttctctact tttattcatc actcttcctt
    96241 gtatgatgtt tattatcttg cccctttcca atatcagtga aaagtatagt cgtcgtttac
    96301 aggaggaaat ttaacagggc aattgactga aaagattcaa gaacatgaat taattaagac
    96361 taaccaagca gagaaaagtg tacaggatgt tttggataat tgtattgaaa gggtacaaaa
    96421 caattcactg aaatcagata gggttacttc ttttgagacc ccgtttgctc tcttatttat
    96481 ctttgcgact atagctgtga tgctaaccta tgggggttat cgggtcagcg caggatatat
    96541 atctgtggga accttggttt cgtttttgat ttacctcttt caattactta atcctattag
    96601 taatatagct aattttgtaa ctgtttattc taggagcaag ggatcttcag ttgcactgga
    96661 taacttgctt gcagttccta aagaaaaatt tgagggagga aaatcggtat caggacaagg
    96721 gttgaatttt aaccatgtct attttggtta tgatgaaaat cgacctgtct taaaggatat
    96781 tacttgttca attttcaagg ggcaaaaatt gcttttgttg gaccatctgg atcaggaaaa
    96841 tcaacgattg tgcgtttgtt agagcggttt tataaaccgc tttcaggaga tattctaatg
    96901 gagcaatcaa gtatatatga ttttaactta aaagaatgga gaagtaaaat cgcttgggtt
    96961 tcacaaaata atgcagtctt atctggcagt attcgtgaca atctttgtct cggtttgaat
    97021 cgcttagtaa ctgatgatga attgatgaaa gtgctagact tagtatcact aggtgatgag
    97081 attcgctcca tgaaagaggg actagatact gaagttggtg aacgcggacg attcttgtca
    97141 ggggggcaaa gccaaagact tcaaatagct agaacctact taaaagatgc tgaaattctt
    97201 atatttgatg aagctactgc taatcttgat gcggattctg agtatgcgat tatcagtagc
    97261 ctctattctg tattaaagga gaagacggtt gtgattatag cgcatagttt gtcaacggta
    97321 aaagatgtgg attgtatttt cttcttagag gaggggaaaa tcactggctc aggaactcat
    97381 aaggaactac tggaaaatca tgagcgttat gctcgttttg tgcaggagca aatgatagag
    97441 tgaagtgtct tttgagattc accattttat agtctattaa agggagcagg aaaaactccc
    97501 tttttatata gtttgaaact ataactagct cttgaaaaga agaaaatgag ttgatgaaaa
    97561 taagtggtac aatagttact atagatttgg aggtattgta tgagcaagga attacacatt
    97621 aacacaattt tggcccaggc gggtattaag tcagatgaag cgacaggtgc attggtgaca
    97681 ccgcttcatt tttcaacgac ctatcagcat ccagagtttg gtcgatctac tgggtttgac
    97741 tatacgcgca ctaaaaatcc aactcgtagt aaggctgagg aagtcttggc gactattgag
    97801 tcagcagact atgccttagc gactagctca gggatgtcag ctattgtact ggcctttagc
    97861 ggctttccag taggaagtaa ggtcttggca gtgcgtgatc tttacggcgg ttcttttcgc
    97921 tggtttaacc aagtggagca ggaaggtcgt ttccatttta cctatgccaa tacagaagaa
    97981 gagttgattg ccgagttaga aaaggatgtg gatgttctct atatcgaaac cccaaccaat
    98041 cccttgatgt tggaatttga tatcgaaaaa ctagcaaaat tggctcatgc taagggtgcc
    98101 aaagtggtgg tggacaatac cttctatagc cctatctacc aacgtccgat tgaagatgga
    98161 gcagatatcg ttctccattc agcaaccaag tatctagcag gccacaatga tgtcttggct
    98221 ggagtggttg tgaccaatag tttagaacta tacgagaagc ttttttacaa tcttaataca
    98281 acaggggcag tcttgtctcc atttgacagc taccagttgc ttcgtggtct caagaccttg
    98341 tctcttcgta tggagcgctc aacagctaac gcccaagaag tggttgcctt tttgaaggat
    98401 tctccagcag ttaaggaagt tctctacact ggtcgtggag gcatgatttc ctttaaagta
    98461 gccgatgaaa cacgcattcc tcatattttg aacagtctca aggtcttctc ttttgcggaa
    98521 agtttgggtg gagtggaaag tcttattact tatccaacga ctcaaactca tgctgatatt
    98581 ccagcagagg tacgccattc ttatggtttg acagatgacc tcttgcgttt gtctattggg
    98641 attgaggatg ctagagattt gattgcagat ttgcgccaag ccatagaagg ataagacaaa
    98701 gatgggaaaa tatgatttta caagcctgcc caaccgttta gggcaccata cctataaatg
    98761 gaaagaaaca gaaacggata gtgaagttct accagcttgg atagcggata tggactttgt
    98821 ggtcttgcct gaaatccgcc aagccgtgca aacttacgca gaccaactgg tttatggtta
    98881 tacctatgcc agtgaagact taattaagga agttcaaaag tgggaagcta cacaatacgg
    98941 ttacaacttt gacaaagagg ctcttgtctt tatcgagggt gtggtaccag ccatctcaac
    99001 agctattcaa acctttacaa aagaaggcga ggcggtttta attaacacgc ctgtctaccc
    99061 accctttgct cgcagtgtca agttgaataa tcgtagattg attactaatt ccttagtgga
    99121 aaaggatggt ctgtttgaga ttgactttga ccaacttgaa aaggatttgg tggaagagga
    99181 ggttaaactc tatattcttt gcaaccctca caatcctggt ggacgtgttt gggaaaaaga
    99241 agtgttggag aagattggcc aactctgcca aaaacacggt gttttgttag tttcggatga
    99301 gattcaccaa gatttgaccc tctttggtca caaacaccag tctttcaata ccatcaatcc
    99361 tgccttcaaa aattttgcta tcgtcttgag cagtgccact aaaacattta atattgctgg
    99421 aacaaaaaat tcctatgcag tcattgaaaa tcctaagttg agactagctt tccagaaacg
    99481 cctgttggcc aataatcagc atgaaatttc aggcttgggt tatttggcga cagaagctgc
    99541 ctatcgatac ggtaaagatt ggctagagga actcaagcaa gtctttgaag accacatcaa
    99601 ttatgtggtg gatctatttg gaaaagagac taaaatcaag gtcatgaaac cgcaaggtac
    99661 ctacttgatt tggcttgact tttcagctta tgacctgact gatgaaacat tgcaagagtt
    99721 gttgagaaat gaagccaagg ttatcctcaa ccgtggtttg gattttggag aggaaggaag
    99781 tctccatgcc cgcatcaata tagctatgcc caaatctctg ttgcaagaag tctgtcagcg
    99841 gattgtggct acttttgcca aacgttaaaa atccagcctt ctaggagaaa agtcttccta
    99901 gaaggctatt ttcataggcg aaaatatggt ataataaaaa gataaggtaa aggtgaaaat
    99961 atggctaaat tgattccggg gaaaattcgt atcgaaggtg ttgcccttta tgaaactggt
   100021 aaggttgata tcatcaagga aaagaacaat cggctctacg ctcgcgttgc agaagaagaa
   100081 ctgcgctata gtttagagga tgatttggtt ttttgtgcct gtgatttttt tcaaaagagg
   100141 ggttactgtg tgcatttggc agcgctagag cattttctga aaaatgatga gcgtggtcag
   100201 gaaatcttgt ggagtctgga agaaggtcat gaagaaaaag aggctgttga aaccaaggtg
   100261 accttgggtg gcaagttttt ggatcgaatt ttatctccaa aatcagaatg cgcctatgag
   100321 ttatcagctg tgggtcaggt ggaagcagga accaatcata tcttgtggac tctgagaatc
   100381 ggccaaatca atagccaaaa atactatgtc attcgggata ttccactttt tttaaggatt
   100441 gtcgagcaga gaaagtcgta tatgattggc aagatttatg aggagtctct ttcttgggaa
   100501 gcctttgatg atgccagcca agaacttttg actttcttgc acggactaac ggaggaaggg
   100561 ctggtttcag acctcttttt ccaaaaccaa ggtcggcacc tctttttccc tctgaccttt
   100621 tttgagcagg gtgtgaattt actgatggcc ttgccccatt ttcaatttga ccatcaagtg
   100681 gatagctacc aaaccctgct ttttcaggat atgcatgctg atgccaatct atttgccttt
   100741 acagtaacag aatactcgga ctattttgaa atggaaatca gtgagagtcc tagggtcaat
   100801 gtcttttatc agggagcagt acttttccat aaaggtcagg tttatttttt aacagaccag
   100861 cagatgcgtc ttctcaagga aattaaagcg ttacctgtgg gtcaacaagg aaagaaatac
   100921 ctgcaatttg atagcagtga tcgagacaaa ttggcctcct gtctgactct ttttatccag
   100981 atgggcaccg tttcagctcc agaacgtcta caaatcaaaa cttttgcgcc ctctttttac
   101041 tttgacaggg aagaagataa tcggattcgt ttagaaattc agtttgatta tggaaataga
   101101 caggtatcta gccgacaaga gctagaagaa ttaccatttt caagtgacgc ggacttagaa
   101161 gaaagaattt tccaagtctg tttggctgcg ggctttgaag ccgattttca atcttggcgt
   101221 caggccttaa aagcggagtc tgtctatcat ttttttcatg atatcattcc agtctttgaa
   101281 aaactcgggc atgttgacct atcagataaa ctagaagagc tttatagcct agcgagtcct
   101341 caggtgcaga ttgcctccaa gggaggtctc ttggaaatcc agtttgattt tcaagatatt
   101401 gcccaggagg aaatcgacca agccatgcag gccttggttg ccaatcagga tttttatatt
   101461 gattcgtcta atcaagttta ctttttcgat gaagaaacca agaaaattcg ccaaaatcta
   101521 caggaactgg ggcaatttga attaaaagat gggaccttgc aggctcggaa atccttggcc
   101581 tacagtttag ctcatctctt tgaagggcga gaccgtgttt ctttttcaca agaattccag
   101641 aatctggccc aggatttgac acatccagag gactttcctc gacaggcaac tcaggttcag
   101701 gctgacttgc gagattatca ggaaaaggga attggctggt tgcagatgct ccatcattat
   101761 ggttttggtg ggattttggc tgatgatatg ggacttggga aaacccttca gaccattgct
   101821 tttttgacta gtcaagtgac aaaagaaagt cgggttttga ttctcgctcc gtcaggtttg
   101881 atttacaact gggcagatga gtttcagaaa tttgccccac agttggatgt ggctgttgtt
   101941 catggtttga aagcaagtcg tgaggagatt cttgccgaga gccatcaaat ctatgtgact
   102001 agctatgcca ccttccgtca ggacagtgag ctttatcagg ggatggcctt tgacttcctt
   102061 ttcttagatg aggctcaggt catgaaaaat gcccagacca agattgccca gaccttgaga
   102121 caatttgtgg tgccgtcggt ctttgccttg tcaggaactc cgattgaaaa ccatctgggt
   102181 gagttgtggt ctattttcca aatcgtcatg cccggacttt tgccaagcaa gaaagaattt
   102241 atgaaattgc cagcagagcg agtggctcag tttatcaagc ctttcgtgat gcgacgcaag
   102301 aaagaagaag ttctgactga attgccagac ttgattgagg tggtttataa aaatgaactg
   102361 gaagaccagc aaaaggctat ttaccttgcc cagttacaac agatgcgaga ccatctggct
   102421 caagtgtcag aacaggaatt tcagcgaagt cgtgtggaaa ttttatctgg tttgatgcgc
   102481 ttgcgtcaaa tatgtgacac tcctgccctg tttatggaag attatcaggg agccagcggc
   102541 aaactagata gtctccgaga tctactggta caggtggcag acggcggaca ccgtgtcttg
   102601 attttctctc agttcaaggg aatgttggaa aaaattgaac aagaactgcc agacttgggc
   102661 ctgacatcct ttaaaattac gggttcaacc ccagccaagg aaagacaaga catgaccaag
   102721 gcctttaacc aaggagaaag agatgccttt ctgatttccc tcaaggctgg tggggtcggt
   102781 ctgaacctga caggtgcaga tacagtgatc ttggttgacc tttggtggaa tcctgcggtg
   102841 gaagcgcaag ccattggccg tgcccatcgg atgggtcagg aagaaacggt tgaggtctat
   102901 cgcttggtga ccaagggaac cattgaagaa aaaattcagg aactccaaga acaaaagaaa
   102961 catctggtgt cacaagtatt ggatggcaca gagtcacgtg gtagtctgac cctagcagaa
   103021 attagagaaa ttttgggaat ttctgaagcc agcacttgaa aaatcaagac aatacgctat
   103081 aatgaagtgt tgaaaggaaa tagaaaatga aacaagaacg atttccattg gtgtcagatg
   103141 acgaggtcat gttgactgaa atgccagtca tgaatctcta tgatgagtct gatctgatca
   103201 gtaatatcaa gggtgagtat cgagataaaa attatttaga atgggctcct attactgaag
   103261 aaaaaccagt aaaaccgatt gaaaagcaag tcgaaaaacc taaaaaggct cctttagggg
   103321 ttaaaaaaga aggaaagagc tatgcggagg tggcgcgtga agaagcgcgt gcggacttga
   103381 aaaagaaacg ctctgctaac tacctaactc aggatttcag ccttgcgaga cgtcattctc
   103441 agcccagtct agttagacag ggcaatcaac cgacaactcc tttccaaaag gaaaatcctg
   103501 gtgaatttgt caaatatagc caaaaattga cccagtctca ttatatcttg gcggaagaag
   103561 ttcattctat ccctaccaag aatgaagaag tgtcagcacc tgctccaaag aaaaacaatt
   103621 atgattttct aaagaagagc caaatctaca ataaaaaaag taaacaaaca gaacaagaac
   103681 gtcgggttgc ccaagagttg aatctgacca gaatgacaga ataggggaga aaacatgtca
   103741 aagacatatc attttatcgg aattaaggga tcagggatga gtgccttggc cttgatgttg
   103801 caccagatgg ggcacaaggt tcagggatca gatgttgaaa agtactactt tacccaacgc
   103861 ggtcttgagc aggcaggaat taccattctt ccttttgatg aaaagaatct agacggtgat
   103921 atggaaatta tcgctggaaa tgcctttcgt ccagataaca acgtcgaaat tgcctatgcg
   103981 gaccaaaatg gtatcagcta caaacgttac catgagtttc taggtagctt tatgcgtgac
   104041 tttgttagca tgggagtagc aggagcacat ggaaaaactt caacgacagg tatgttgtct
   104101 catgtcttgt ctcacattac agataccagc ttcttgattg gagatgggac aggtcgtggt
   104161 tcggctaatg ccaaatattt tgtctttgaa tctgacgaat atgagcgtca cttcatgcct
   104221 taccacccag aatactctat tatcaccaac attgactttg accatccaga ttatttcaca
   104281 agtctcgagg atgttttcaa tgcctttaac gactatgcca aacaaattac caagggtctt
   104341 tttgtctatg gtgaagatgc tgaattgcgt aagattacgt ctgatgcacc aatttattat
   104401 tatggttttg aagctgaagg caatgacttt gtagctagtg atcttcttcg ttcaacaact
   104461 ggttcaacct tcaccgttca tttccgtgga caaaacttgg ggcaattcca cattccaacc
   104521 tttggtcgtc acaatatcat gaatgcgaca gccgttattg gtcttcttta cacagcagga
   104581 tttgatttga acttggtgcg tgagcacttg aaaacatttg ccggtgttaa acgtcgtttc
   104641 actgagaaaa ttgtcaatga tacagtgatt attgatgact ttgcccatca tccaacagaa
   104701 attattgcga ccttggatgc ggctcgtcag aaatacccaa gcaaggaaat tgtagcagtc
   104761 tttcaaccgc atacctttac aagaaccatt gccctgttgg acgactttgc ccatgcttta
   104821 aaccaagcag atgctgttta tctagcgcaa atttatggct cggctcgtga agtagatcat
   104881 ggtgacgtta aggtagaaga cctagccaat aaaatcaaca aaaaacacca agtgattact
   104941 gttgaaaatg tttctccact cctagaccat gacaatgctg tttacgtctt tatgggagca
   105001 ggagacatcc aaacctatga atactcattt gagcgtctct tgtctaactt gacaagcaat
   105061 gttcaatagg atgttcccat ggaaattcca attaagatca ttcaggcaag caagtttgat
   105121 ttgcctgaga taggggcact tcagacctcg tcttttccag ctgaaaagca gcaactttcc
   105181 catattttag aagaaagtat ccgtaagtgt gcggatacct ttcttctagc tagggatgaa
   105241 aatcaacttt taggctatat tttatcaagt ccccagtcag acaatccgca atgtctaaaa
   105301 gtacattctt tagtcatcga gtctgaccat cagagacagg gcctgggaac acttcttctt
   105361 gcagccttga aagaggtggc agttgagctg gattacaaag ggattcgttt ggagagtcct
   105421 gatgagctgc tttcctattt tgaaatgaac ggttttgttg atgaagaaga aattttgctc
   105481 tatgcaacta gccagggcta tagtatgatt tggtttaatc ccttttatct ggaggaacaa
   105541 tgaaaatcag acaagcaaga ttagaagatt tggatcggat tgttgaactt gaatttgaaa
   105601 atttttcggt cgaagaagcc attcctcctt ctgtctttga agcacatttg agagaaattc
   105661 agacctcttt tctggttgct gaaaaagaag gaagaatcat gggttatatc gaaggaccag
   105721 ttggcctgca ccgccatctg caagaccagt cttttacaga agaaataaaa gactatagtc
   105781 atgagcctgg tggttatatc tctgtgacct gtctgtctat tgccaaggag gcacagggac
   105841 tcggactggg tcagaaattg ctgacagcct tgaaagaagt tgctcttgaa gatgagagag
   105901 atggcattaa tctaacctgt catgactatc tcatcgccta ttatgaaaaa catggatttg
   105961 tcaacgaagg ccagtcccag tcaacctttg caggggaaac atggtatgat atggtctggg
   106021 aaatgaaaaa ataagttagg aaaagtatca tagattgctt tttttggact tttaagatat
   106081 aatattatgg attatcaaca aggaggaaaa acttttgagt gaaaagtcaa gagaagaaga
   106141 aaaattaagc tttaaagagc agattctgag agatttagaa aaagtaaaag gctatgatga
   106201 agttctgaaa gaagatgagg cagtagttcg cactcctgca aatgaacctt cagctgaaga
   106261 actcatggct gattccttgt caacggtaga ggagattatg agaaaagctc ctaccgtgcc
   106321 tactcaccca agtcaaggtg taccagcttc tccagcagat gagattcaaa gagaaactcc
   106381 tggtgttcca agtcatccaa gtcaagatgt accttcttct ccagcggaag aaagtggatc
   106441 aagaccaggt ccaggtcctg ttagacctaa gaaacttgaa agagaataca atgaaacccc
   106501 aacaagggta gctgtttcct atacgacggc agagaaaaaa gcagaacaag caggtccaga
   106561 aacacctacg cctgctacag aaacagtgga tatcatcaga gatacatcac gtcgtagccg
   106621 tagagaagga gcaaaacccg ttaagcctaa gaaagagaag aagtcacatg tgaaagcttt
   106681 tgtgatttca ttccttgtat tccttgcctt gctctcagca ggtggttact ttggttacca
   106741 gtacgtgcta gattccttat tacctatcga tgctaattct aagaaatatg tgacggttgg
   106801 aattccagaa ggttcaaacg ttcaagaaat cggtacgacg cttgaaaaag ctggtttggt
   106861 aaagcatggt ctgattttta gtttttatgc caagtataaa aattataccg acttgaaagc
   106921 aggttactac aatttgcaaa agagtatgag tacagaagac ttactcaaag agttgcaaaa
   106981 aggtggaaca gatgaaccgc aagaacctgt acttgcgact ttgacaattc cagaaggtta
   107041 taccttggat cagattgctc aaactgtggg tcaattgcaa ggtgacttca aagagtcttt
   107101 gacagcggag gctttcttgg ctaaagttca agatgagacg tttatcagtc aagcagtagc
   107161 gaaatatcct actttactgg aaagtttgcc tgtaaaagac agcggtgcgc gttatcgttt
   107221 ggaaggatac cttttcccag ctacatactc tatcaaggaa agcacaacta ttgagagctt
   107281 gattgatgag atgttagctg ctatggataa gaacctatct ccttactata gtactatcaa
   107341 atctaaaaac ttgactgtca atgagttgtt gaccattgct tccttggtcg aaaaagaagg
   107401 tgccaagaca gaagatcgta agctcattgc aggtgtattc tacaatcgtt tgaatcgtga
   107461 tatgccactt caaagtaata ttgcaatctt gtatgcccaa ggaaaactgg ggcaaaatat
   107521 cagtctagct gaggatgttg cgattgatac caacattgat tcaccttata atgtttataa
   107581 aaatgtaggt ctcatgcctg gtccagtcga tagtccaagt ctggatgcga ttgagtcaag
   107641 catcaatcaa actaagagcg ataacctcta ctttgtagca gatgtcacag aaggcaaggt
   107701 ctactatgct aacaatcaag aagaccacga ccgcaatgtc gctgaacatg tcaacagcaa
   107761 attaaactaa acaaactaaa attatgtgat acttcacata attttcttta gaaaatatta
   107821 tcagaagaaa gttgagaaaa atggcagaaa aaacatatcc tatgaccctt gaggaaaagg
   107881 agaaacttga aaaagaatta gaagaattga aattggttcg tcgaccagaa gtggtagaac
   107941 gcattaagat tgcccgttca tacggtgatc tttcagaaaa cagtgagtac gaagcagcta
   108001 aggatgaaca agcctttgtc gaaggacaaa tctctagctt agaaacaaaa atccgctatg
   108061 ctgaaatcgt caatagcgac gcagttgccc aggacgaagt agcgattggt aaaacagtca
   108121 ccatccaaga aattggtgag gacgaagaag aagtttatat tatcgtaggt tcagctggtg
   108181 cggatgcctt tgcaggtaag gtttcaaatg aaagcccaat tgggcaggcc ttgattggca
   108241 agaaaacagg tgacacagca accattgaaa cgcctgttgg tagctatgat gtaaaaatct
   108301 tgaaggttga aaaaacagcc taaaaacaga aaaaggagtg gggaggcgat gtgcttcact
   108361 cactcctttt tccattttgc tattcttcga aaatctcttc aaaccacgtc agcgtcgcct
   108421 tgccgtatgt atggttactg actttgtcag tttcatttac aacctcaaaa cagtgttttg
   108481 agctaacttc gtcagtttca tctacaacct caaaactatg ttttgagctg acttcgtcag
   108541 tttcatctac aacctcaaaa ccatgttttg agctgacttc gtcagttcta tctacaacct
   108601 caaaacagtg ttttgagcaa cctgcggcta gtttcctagt ttgctctttg attttcattg
   108661 agtattagat gtatagtgca ttgaagttaa aatagtaaac tttgatttct aagacattat
   108721 tagaaattag attaaattcc ttaatcgatt tgcgcatgtt ttatttcatt tcattatact
   108781 ttagattgga aaataaggaa tcacataaat cgcaagaaat agggaatctg attcgtgttt
   108841 ataaccgttc aaaaagggaa gaggctgaaa gtgagccact taatctttat gtcgaagatg
   108901 aaaagggcaa tctccttgcg ggtttgatag cagagacttt tggaaattgg ctagaaatcg
   108961 agtatttgtt tgtaaaagag gaactgcgag ggcaaggaat cggttcaaaa ctattgcaac
   109021 aggcagaaag tgaagctaag aatcgaaatt gttgttttgc ctttgtcaat acttatcagt
   109081 tccaggcacc ggacttttat cagaaacatg gctacaagga agttttttct ttgcaagact
   109141 atctctacat taggcaaaga tattattacc aaaagaatct gtaaggtgag tatggaggta
   109201 taatacaaag agaggctcgc acataagtat ggaggtggac tgatgtatat gagtaactat
   109261 cgataaatac cagttataac ctagaaattg tgtctttcta gacgatattg aggacaatat
   109321 aatcgtagct ccaaaattag gaatcaaggc ttatcaggtt aagaaaagaa gtgatgttgt
   109381 tgatatttta aaacaattta tttaaactaa aaactctcta tctttttaaa ttgacattaa
   109441 tccgagattc ccctctcgga ttttgtgttg ctttcattaa atttacaggt aagacttgct
   109501 agcctcagta aatatacaca agtttttcag aatagagtga aatccgaaag aaaatggaaa
   109561 taatgaaagc tcttgtaggc ataagaaaac acctctgtgt tatacttgtt gttcaaccac
   109621 aaacacaaga aaggcacaga gatgcaaaac cattatacta caaaaggcga acatttgaca
   109681 atcgatagcc gtcgcttaat cgaaagatgg aaaaaagaag ggaaatcaaa tagagaaatt
   109741 gcctctct
//
LOCUS       DAVDWT010000003        91527 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVDWT010000003 DAVDWT010000000
VERSION     DAVDWT010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 91527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91527
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.003"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(123..908)
                     /locus_tag="VUW50_000241"
                     /pseudo
     CDS             complement(123..908)
                     /locus_tag="VUW50_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1157..2602
                     /gene="cps4A"
                     /locus_tag="VUW50_000242"
     CDS             1157..2602
                     /gene="cps4A"
                     /locus_tag="VUW50_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HET7982209.1"
                     /translation="MSRRFKKSRSQRVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSDIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAIIQKLTST
                     EVLKNYSSILQGLQDSLQTNMPIETMIDLVNTQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            2604..3335
                     /locus_tag="VUW50_000243"
     CDS             2604..3335
                     /locus_tag="VUW50_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HET7982210.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKVKELFVDNPRKIIMDQLI"
     gene            3344..4036
                     /gene="cpsC"
                     /locus_tag="VUW50_000244"
     CDS             3344..4036
                     /gene="cpsC"
                     /locus_tag="VUW50_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HET7982211.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSMTRIYVVNRNQGEKSGLTNQDLQAGSSLVKDYREIILSQDVLEEVVSDLKLD
                     LTPKDLANKIKVTVPVDTRIVSVSVSDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPATSPSSPNIKRSTLIGFLAGVIGTSVIVLILELLDTRVKRPKDIEDTLHMT
                     LLGIVPNLNKLK"
     gene            4046..4735
                     /gene="cpsD"
                     /locus_tag="VUW50_000245"
     CDS             4046..4735
                     /gene="cpsD"
                     /locus_tag="VUW50_000245"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HET7982212.1"
                     /translation="MPTLEIAQKKLEFVKKAEEYYNALCTNIQLSGDKLKVISVTSVN
                     PEEGKTTTSVNIARSFARAGYKTLLIDGDTRNSVMSGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGTVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTAPIGIVI
                     DAAIITQKCDASILVTATGEVNKRDVQKAKQQLEQTGKLFLGVVLNKLDISVDKYGVY
                     GFYGNYGKKII"
     gene            4746..6113
                     /locus_tag="VUW50_000246"
     CDS             4746..6113
                     /locus_tag="VUW50_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET7982213.1"
                     /translation="MDEKGLKIFLAVLQSIIVILLVYFLSFVRETELERSSMVILYLL
                     HFFVFYFSSYGNNFFKRGYLVEFNSTIRYIFFFAIAISVLNFFIAERFSISRRGMVYF
                     LTLEGISLYLLNFLVKKYWKHVFFNLKNSKKILLLTVTKNMEKVLDKLLESDELSWKL
                     VAVSVLDKSDFQHDKIPVIEKEKIIEFATHEVVDEVFVNLPGESYDIGEIISRFETMG
                     IDVTVNLKAFDKNLGRNKQIHEMVGLNVVTFSTNFYKTSHVIAKRIIDIMGALVGLIL
                     CGLVSIVLVPLIRKDGGSAIFAQTRIGKNGRQFTFYKFRSMCVDAEAKKRELMEQNTM
                     QGGMFKVDDDPRITKIGRFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYVQYTSE
                     QKRRLSFKPGITGLWQVSGRSKITDFDDVVKLDVAYIDNWTIWKDIEILLKTVKVVFM
                     RDGAK"
     gene            6117..6860
                     /locus_tag="VUW50_000247"
     CDS             6117..6860
                     /locus_tag="VUW50_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HET7982214.1"
                     /translation="MRDRIQLLGVTIDLLTMNETIDSVEQYVLEKRPLHLMGVNADKI
                     NQCHTDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMQCLLELSNKKG
                     YSVYFFGAKEEVLQDMLKVFKRDYPNLIVIGHRNGYFSEEDEQAIQEDIREKNPDFVF
                     IGITSPKKEYIIQKFMDSGVNSVFMGVGGSFDVLSGHIQRAPLWMQKSNLEWLFRVAN
                     EPKRLFKRYFVGNISFIGKVLKAKRGVKY"
     gene            6857..7681
                     /locus_tag="VUW50_000248"
     CDS             6857..7681
                     /locus_tag="VUW50_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HET7982215.1"
                     /translation="MNQTEMIRLIQKVELDAIKEFKKICEENDIDFFLRGGSVLGAVK
                     YDGFIPWDDDMDIAVPREAYDKLPSVFKDRIIAGKYQVLTYQYCDTLHCYFPRLFLLE
                     DERKRLGLPRNTNLGLHLIDIIPLDGAPNHSVLRKIYFGKVYWYRFLASLGTTYVGDH
                     VDMHSTKQKLIIGFFKKLGFAKLFPQNSVYRRLDNLYRKYDWKKQKYAGTINASLFAK
                     EVMPVEIWGEGVEKPFEDTFFKVPTEYDRYLKRLYGENYLYEEPSDDEKKSHLGGH"
     gene            7683..8561
                     /locus_tag="VUW50_000249"
     CDS             7683..8561
                     /locus_tag="VUW50_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7982216.1"
                     /translation="MFCYIILHYKVLEETISCVKSIKEGNYNAKQIVIIDNFSNNGTG
                     EKLQELYESDLEIDVLINHENAGFARGNNVAYQFAKEKYNPDFMVIMNNDIEIETENF
                     EKIVTDIYREEKFHLLGPDIFSTTYQLHQNPKRLTHYTYGEVKALNEKFKKGSQVSLA
                     LKIKCWLKASKVLRTAIYQNRRKKGSVDYRKQVENPILHGSFIVYSRDFIEKEEYAFN
                     PNTFFYYETEILDYEAELKGYKRIYTPKIRVLHHQNVATNQVYTNLLEKTLFSNKCNF
                     KSTSYFLKLMKENEDV"
     gene            8562..9899
                     /locus_tag="VUW50_000250"
     CDS             8562..9899
                     /locus_tag="VUW50_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HET7982217.1"
                     /translation="MSYLFLLCLTLFLLTIFYFFAFIQDLIAPPVVMSVMFLISSVFA
                     LVNSKNWNIEYSGIAYILIISGIIIFSIPLMALKSPNFNTEVKIADRLIDIQFWKIAL
                     TIIIDLFILYLYRKEIYNLVLSNGYTGSNIQWFFRNATSYEGELTVRTFIRVLIRVID
                     VSAYIFGYTFINNFLIYRHKRPKDILLLVPLLIFISKTLISGGRQDIIKILIAYVIMM
                     YIQQKRKVGWNRVISHKYIHLGFVGLIAGIPAFYYSLFLAGRSTTRTLFESVSTYLGG
                     SIQHFNQYIENPLDPGEVFGSETLVPILNILGEMGLVNYRSTIHLEFRTLGVTVGNVY
                     TFFRRPLHDFGLVGMYVFVFAVGAFFAIYYLVLRKKQVGFNLDIHTIIYSYVFYWIFL
                     SSIEQYSFTMISLYTLVFIVLVYFMAIFYWCTDFKRGKLIFKISDSSIKLKEE"
     gene            9922..11343
                     /locus_tag="VUW50_000251"
     CDS             9922..11343
                     /locus_tag="VUW50_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982218.1"
                     /translation="MNTKIKNIITSFSYVISSNLLIVLTSSLVVLIFPKIMGVTEYSY
                     WQLYIFYLTYIGFFHLGWIDGIYLKYGGLEYTNLDRKQFYSQMILFSSFLMLISLVLF
                     TLNLITVRDENARYIYNMAIISMIVTNLRTLYVYILQMTNRLKDSSVILISDRVLYVL
                     LLFMFIVFGWHEYKVMIWADILGRTFSLMLSFWICKDIVFQPLSKFILDFKESLDNIR
                     VGINLMLSNIASSMIIGIVRMGIQWNWNIETFGKVSLTLSISNLLMTFINAIGLVIFP
                     LIKRTKTENLPKIYSNLRNALMLVMFAILLFYYPLKFILDIWLPAYKDALVFMALIFP
                     MSVYEGKMALVINTYLKAMRMEKDILKINALVMLTSIVVTLVTTILLNNLGLTVVSIV
                     ILLALRSIIAELILSKKLKISVKQDIALELLMTIIFISSSWYLSIWIAVIIYLLAYTL
                     YLYLKHKDIRMYIEYFKNHKKIS"
     gene            11407..12495
                     /gene="wecB"
                     /locus_tag="VUW50_000252"
     CDS             11407..12495
                     /gene="wecB"
                     /locus_tag="VUW50_000252"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HET7982219.1"
                     /translation="MKKIMLVFGTRPEAIKMCSLVNELRKQEDMKTVVCVTGQHKEMV
                     SPVLDLFGVQPDYDLEIMKANQNLFSITISILEKIKPVLEKEQPDIVLVHGDTTTTYA
                     AALAAFYLGIKVGHVEAGLRTYNLQSPFPEEFNRQSTSIIANYHFAPTELAKENLIKE
                     GRNNIYVTGNTVIDALTTTVQKDYTHPDLDLNDGNRLILLTAHRRENLGEPMRHMFRA
                     VKRVLNEYDDVKVIYPIHKNPLVRETATEIFGDTERIQIIEPLDVLDFHNFMNHSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVAAGTLKLVGTDEETIYQNFKMLLDDPE
                     EYKKMSQASNPYGNGDASKQIVRILRGI"
     gene            12534..13403
                     /gene="rfbA"
                     /locus_tag="VUW50_000253"
     CDS             12534..13403
                     /gene="rfbA"
                     /locus_tag="VUW50_000253"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET7982220.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGLKLSYAEQPSPDGLAQAFIIGEEFISDDSV
                     ALILGDNIYHGSGLSKMLQKAASKESGATVFGYHVKDPERFGVVEFDQDMKAISIEEK
                     PEQPRSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKN
                     EYGQYLLRLIGEA"
     gene            13404..14000
                     /locus_tag="VUW50_000254"
     CDS             13404..14000
                     /locus_tag="VUW50_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HET7982221.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENIAEAEVSEADKHHPLLKDVKPLKKEDLE"
     gene            14010..15059
                     /gene="rfbB"
                     /locus_tag="VUW50_000255"
     CDS             14010..15059
                     /gene="rfbB"
                     /locus_tag="VUW50_000255"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET7982222.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLAAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQAAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            15125..15976
                     /gene="rfbD"
                     /locus_tag="VUW50_000256"
     CDS             15125..15976
                     /gene="rfbD"
                     /locus_tag="VUW50_000256"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HET7982223.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITDAEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVARASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            16052..16486
                     /locus_tag="VUW50_000257"
     CDS             16052..16486
                     /locus_tag="VUW50_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7982224.1"
                     /translation="MLLVLVSLAHSFGSVSIIAHTIHQKFNLKVPNYRQEEDWARMGL
                     PITRKEISNWHIKASQYYLESLYNLLREKLLEQPLLHADETSYRVLESDSQLTYYWTF
                     LSGKAENQVITLYHHDQCRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            16559..16816
                     /locus_tag="VUW50_000258"
     CDS             16559..16816
                     /locus_tag="VUW50_000258"
                     /inference="COORDINATES: protein motif:HMM:NF015038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7982225.1"
                     /translation="MVNCEPLEAYRQLEEAELVGCWAHVRRKFFEVPPKQADKSSLGA
                     KGLAYCDQLFSLERDWEALPADERLQKRQEHLQPLMEDFFA"
     gene            16862..17008
                     /locus_tag="VUW50_000259"
     CDS             16862..17008
                     /locus_tag="VUW50_000259"
                     /inference="COORDINATES: protein motif:HMM:NF015038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7982226.1"
                     /translation="MEYSLKYEETFKTILKDGHLVLSNNLAERAIKSLVMGRSKRVQW
                     TLLA"
     gene            17079..17237
                     /locus_tag="VUW50_000260"
     CDS             17079..17237
                     /locus_tag="VUW50_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HET7982227.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            17436..19418
                     /locus_tag="VUW50_000261"
     CDS             17436..19418
                     /locus_tag="VUW50_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7982228.1"
                     /translation="MKSSKLLALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDGYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVMAPVNEEFLNSKGDDFAKATDPNSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPILYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            19718..25036
                     /locus_tag="VUW50_000262"
     CDS             19718..25036
                     /locus_tag="VUW50_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HET7982229.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQ
                     NDNAGKPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYD
                     KDGWFWEYKTPGNSTWYKGNRVAAPETGSINRLSITLKSDGQLNASNNDVNLFDTVTL
                     PAAVNDHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKV
                     TYDTIQSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKIN
                     ETTAEYLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLL
                     SSISFLGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVS
                     TDKLAAGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFP
                     EYTKELPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFG
                     SQAQNPFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDF
                     KTLIEKAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDA
                     AYDLAHGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRF
                     AIEWGHGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAA
                     NYPLLGGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDN
                     GSTYKWTPEMRVELVDANNNKVVVTRKSNDVNSPQYRKRTVTLNGRVIQDGSAYLTPW
                     NWDANGKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVK
                     DGKITLDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEI
                     VKSQGANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKE
                     VTTYTNKSLALNYVKAYAHNTRRDNATVDNTSYFQNMYAFFTTGSDVSNVTLTLSREA
                     GDQATYFDEIRTFENNSSMYGENHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRT
                     HLSEKHDPYTQRGWNGKKVDDVIDGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYR
                     VTFEYEAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTG
                     AETGDTWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALK
                     NYLPTVAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTA
                     LVADDFASLTAPAQAQEGLANAFDGNVSSLWHTSWGGGDVGKPATMVLKEPTEITGLR
                     YVPRGSGSNGNLRDVKLVVTDESGKEHTFTATDWPNNNKPKDIDFGKTIKAKKIVLTG
                     TKTYGDGGDKYQSAAELIFTRPQVAETPLDLSGYEAALVKAQKLTDKDNQEEVASVQA
                     SMKYATDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLRSLASEQGKTPDY
                     KQEIARPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(25203..27362)
                     /locus_tag="VUW50_000263"
     CDS             complement(25203..27362)
                     /locus_tag="VUW50_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET7982230.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKVQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(27359..27955)
                     /gene="recU"
                     /locus_tag="VUW50_000264"
     CDS             complement(27359..27955)
                     /gene="recU"
                     /locus_tag="VUW50_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HET7982231.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            28021..28548
                     /locus_tag="VUW50_000265"
     CDS             28021..28548
                     /locus_tag="VUW50_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HET7982232.1"
                     /translation="MATALVLGYSTFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            28618..28947
                     /gene="gpsB"
                     /locus_tag="VUW50_000266"
     CDS             28618..28947
                     /gene="gpsB"
                     /locus_tag="VUW50_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HET7982233.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            28968..29348
                     /gene="rnpB"
                     /locus_tag="VUW50_000267"
     ncRNA           28968..29348
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VUW50_000267"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            29433..30590
                     /locus_tag="VUW50_000268"
     CDS             29433..30590
                     /locus_tag="VUW50_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET7982234.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            30603..31997
                     /gene="mapZ"
                     /locus_tag="VUW50_000269"
     CDS             30603..31997
                     /gene="mapZ"
                     /locus_tag="VUW50_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HET7982235.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTNSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNDTRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            32073..33497
                     /gene="gndA"
                     /locus_tag="VUW50_000270"
     CDS             32073..33497
                     /gene="gndA"
                     /locus_tag="VUW50_000270"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET7982236.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            33509..34198
                     /locus_tag="VUW50_000271"
     CDS             33509..34198
                     /locus_tag="VUW50_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7982237.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            34296..35261
                     /gene="cbpC"
                     /locus_tag="VUW50_000272"
     CDS             34296..35261
                     /gene="cbpC"
                     /locus_tag="VUW50_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HET7982238.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDPETGIM
                     RTGWQYLGNNWYYLRSSGAMATGWKYVRGKWYYLDNKNGDMKTGWYQDGSTWYYLDPS
                     NGDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            35280..36278
                     /gene="cbpJ"
                     /locus_tag="VUW50_000273"
     CDS             35280..36278
                     /gene="cbpJ"
                     /locus_tag="VUW50_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HET7982239.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLATNTVFADNSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKELEIKTQDSVGRKYGEKRENSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYIGGERRAGWINDASAWYYLDPATGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(36396..37628)
                     /locus_tag="VUW50_000274"
     CDS             complement(36396..37628)
                     /locus_tag="VUW50_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7982240.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEIVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTLFLVLSPFSLI
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVMSIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFVTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(37787..38257)
                     /locus_tag="VUW50_000275"
     CDS             complement(37787..38257)
                     /locus_tag="VUW50_000275"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982241.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCGIAY
                     ALFAYSKVLEPSMFYNDLHTFHAELKKLLEKVTSNTENLGNLQLSWCEGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKSNR"
     gene            complement(38157..38495)
                     /locus_tag="VUW50_000276"
     CDS             complement(38157..38495)
                     /locus_tag="VUW50_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
                     /protein_id="HET7982242.1"
                     /translation="MTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANV
                     TAKKLIENYDTLEEIDFALGKSGVLLSLIKYYQFTNDNTLKTFIHNSIWGNLSLFPTK
                     RYSQRKHFRL"
     gene            39158..39436
                     /locus_tag="VUW50_000277"
     CDS             39158..39436
                     /locus_tag="VUW50_000277"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7982243.1"
                     /translation="MIRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSAQVPWQ"
     gene            39611..40489
                     /gene="mvk"
                     /locus_tag="VUW50_000278"
     CDS             39611..40489
                     /gene="mvk"
                     /locus_tag="VUW50_000278"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HET7982244.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            40471..41424
                     /gene="mvaD"
                     /locus_tag="VUW50_000279"
     CDS             40471..41424
                     /gene="mvaD"
                     /locus_tag="VUW50_000279"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET7982245.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            41411..42418
                     /locus_tag="VUW50_000280"
     CDS             41411..42418
                     /locus_tag="VUW50_000280"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HET7982246.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAESTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            42402..43412
                     /locus_tag="VUW50_000281"
     CDS             42402..43412
                     /locus_tag="VUW50_000281"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HET7982247.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            43490..44188
                     /locus_tag="VUW50_000282"
     CDS             43490..44188
                     /locus_tag="VUW50_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HET7982248.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            44185..45180
                     /locus_tag="VUW50_000283"
     CDS             44185..45180
                     /locus_tag="VUW50_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7982249.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSMTCLLVLFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            45194..45826
                     /locus_tag="VUW50_000284"
     CDS             45194..45826
                     /locus_tag="VUW50_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7982250.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            45827..46069
                     /locus_tag="VUW50_000285"
                     /pseudo
     CDS             45827..46069
                     /locus_tag="VUW50_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            45994..46305
                     /locus_tag="VUW50_000286"
     CDS             45994..46305
                     /locus_tag="VUW50_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET7982251.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            46330..46596
                     /locus_tag="VUW50_000287"
                     /pseudo
     CDS             46330..46596
                     /locus_tag="VUW50_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            46746..46985
                     /locus_tag="VUW50_000288"
     CDS             46746..46985
                     /locus_tag="VUW50_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982252.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            47066..47891
                     /gene="cbpG"
                     /locus_tag="VUW50_000289"
                     /pseudo
     CDS             47066..47891
                     /gene="cbpG"
                     /locus_tag="VUW50_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
     gene            47910..48932
                     /gene="cbpF"
                     /locus_tag="VUW50_000290"
                     /pseudo
     CDS             47910..48932
                     /gene="cbpF"
                     /locus_tag="VUW50_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
     gene            complement(49060..50195)
                     /locus_tag="VUW50_000291"
                     /pseudo
     CDS             complement(49060..50195)
                     /locus_tag="VUW50_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            50404..52173
                     /locus_tag="VUW50_000292"
     CDS             50404..52173
                     /locus_tag="VUW50_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HET7982253.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFLEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGASPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            52197..54152
                     /locus_tag="VUW50_000293"
     CDS             52197..54152
                     /locus_tag="VUW50_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET7982254.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREKEEVKRCLLMLMSKEASEEVRDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            54154..54591
                     /locus_tag="VUW50_000294"
     CDS             54154..54591
                     /locus_tag="VUW50_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7982255.1"
                     /translation="MKLEKHLIKLDKQFSNKEEAIRYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPRGVDFGNESNTQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            54653..55789
                     /locus_tag="VUW50_000295"
     CDS             54653..55789
                     /locus_tag="VUW50_000295"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HET7982256.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQVLDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
     gene            56575..57858
                     /locus_tag="VUW50_000296"
     CDS             56575..57858
                     /locus_tag="VUW50_000296"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET7982257.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDQKFGEEALYQDAMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEDRIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     ATAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(57906..60272)
                     /locus_tag="VUW50_000297"
     CDS             complement(57906..60272)
                     /locus_tag="VUW50_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HET7982258.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGVALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKRDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(60551..61165)
                     /gene="lepB"
                     /locus_tag="VUW50_000298"
     CDS             complement(60551..61165)
                     /gene="lepB"
                     /locus_tag="VUW50_000298"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET7982259.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            complement(61169..62050)
                     /locus_tag="VUW50_000299"
     CDS             complement(61169..62050)
                     /locus_tag="VUW50_000299"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HET7982260.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQLVEENRGQNLPLIGTDEVGNGSYFGGLAVVASFV
                     TPDQHAFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            62137..62439
                     /gene="zapA"
                     /locus_tag="VUW50_000300"
     CDS             62137..62439
                     /gene="zapA"
                     /locus_tag="VUW50_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET7982261.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            62436..62984
                     /locus_tag="VUW50_000301"
     CDS             62436..62984
                     /locus_tag="VUW50_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET7982262.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEDQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            63088..65424
                     /locus_tag="VUW50_000302"
     CDS             63088..65424
                     /locus_tag="VUW50_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HET7982263.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHATEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     regulatory      65587..65675
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            65766..67088
                     /locus_tag="VUW50_000303"
     CDS             65766..67088
                     /locus_tag="VUW50_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HET7982264.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     gene            67306..67854
                     /locus_tag="VUW50_000304"
     CDS             67306..67854
                     /locus_tag="VUW50_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7982265.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            67964..68863
                     /locus_tag="VUW50_000305"
     CDS             67964..68863
                     /locus_tag="VUW50_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HET7982266.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(68888..70162)
                     /gene="serS"
                     /locus_tag="VUW50_000306"
     CDS             complement(68888..70162)
                     /gene="serS"
                     /locus_tag="VUW50_000306"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET7982267.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILIK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDASVTIPEALRPYMGGAEVIKP"
     gene            complement(70376..70747)
                     /locus_tag="VUW50_000307"
     CDS             complement(70376..70747)
                     /locus_tag="VUW50_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HET7982268.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(70750..72114)
                     /locus_tag="VUW50_000308"
     CDS             complement(70750..72114)
                     /locus_tag="VUW50_000308"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET7982269.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            72483..73268
                     /locus_tag="VUW50_000309"
     CDS             72483..73268
                     /locus_tag="VUW50_000309"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET7982270.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            73362..73796
                     /locus_tag="VUW50_000310"
     CDS             73362..73796
                     /locus_tag="VUW50_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7982271.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            73796..74770
                     /locus_tag="VUW50_000311"
     CDS             73796..74770
                     /locus_tag="VUW50_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HET7982272.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLIFTI"
     gene            74830..75054
                     /locus_tag="VUW50_000312"
     CDS             74830..75054
                     /locus_tag="VUW50_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7982273.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            75173..76147
                     /gene="fabK"
                     /locus_tag="VUW50_000313"
     CDS             75173..76147
                     /gene="fabK"
                     /locus_tag="VUW50_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HET7982274.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            76140..77060
                     /gene="fabD"
                     /locus_tag="VUW50_000314"
     CDS             76140..77060
                     /gene="fabD"
                     /locus_tag="VUW50_000314"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET7982275.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVVLVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            77094..77825
                     /gene="fabG"
                     /locus_tag="VUW50_000315"
     CDS             77094..77825
                     /gene="fabG"
                     /locus_tag="VUW50_000315"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET7982276.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            77847..79082
                     /gene="fabF"
                     /locus_tag="VUW50_000316"
     CDS             77847..79082
                     /gene="fabF"
                     /locus_tag="VUW50_000316"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET7982277.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            79085..79570
                     /locus_tag="VUW50_000317"
     CDS             79085..79570
                     /locus_tag="VUW50_000317"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET7982278.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            79567..79989
                     /gene="fabZ"
                     /locus_tag="VUW50_000318"
     CDS             79567..79989
                     /gene="fabZ"
                     /locus_tag="VUW50_000318"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET7982279.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            80001..81368
                     /gene="accC"
                     /locus_tag="VUW50_000319"
     CDS             80001..81368
                     /gene="accC"
                     /locus_tag="VUW50_000319"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET7982280.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASRNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            81405..82271
                     /locus_tag="VUW50_000320"
     CDS             81405..82271
                     /locus_tag="VUW50_000320"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HET7982281.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            82268..83035
                     /locus_tag="VUW50_000321"
     CDS             82268..83035
                     /locus_tag="VUW50_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HET7982282.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKHAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGSRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            83298..83483
                     /gene="briC"
                     /locus_tag="VUW50_000322"
     CDS             83298..83483
                     /gene="briC"
                     /locus_tag="VUW50_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HET7982283.1"
                     /translation="MTATETFTVISTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            83965..84201
                     /locus_tag="VUW50_000323"
     CDS             83965..84201
                     /locus_tag="VUW50_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7982284.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(84251..84673)
                     /gene="nusB"
                     /locus_tag="VUW50_000324"
     CDS             complement(84251..84673)
                     /gene="nusB"
                     /locus_tag="VUW50_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET7982285.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(84666..85055)
                     /locus_tag="VUW50_000325"
     CDS             complement(84666..85055)
                     /locus_tag="VUW50_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET7982286.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(85077..85637)
                     /gene="efp"
                     /locus_tag="VUW50_000326"
     CDS             complement(85077..85637)
                     /gene="efp"
                     /locus_tag="VUW50_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET7982287.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(85766..87208)
                     /gene="gatB"
                     /locus_tag="VUW50_000327"
     CDS             complement(85766..87208)
                     /gene="gatB"
                     /locus_tag="VUW50_000327"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET7982288.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISNEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(87208..88674)
                     /gene="gatA"
                     /locus_tag="VUW50_000328"
     CDS             complement(87208..88674)
                     /gene="gatA"
                     /locus_tag="VUW50_000328"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET7982289.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLEDIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            complement(88674..88976)
                     /gene="gatC"
                     /locus_tag="VUW50_000329"
     CDS             complement(88674..88976)
                     /gene="gatC"
                     /locus_tag="VUW50_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET7982290.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            89189..90733
                     /locus_tag="VUW50_000330"
     CDS             89189..90733
                     /locus_tag="VUW50_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET7982291.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            91016..91213
                     /locus_tag="VUW50_000331"
     CDS             91016..91213
                     /locus_tag="VUW50_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET7982292.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            91324..91500
                     /locus_tag="VUW50_000332"
     CDS             91324..91500
                     /locus_tag="VUW50_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET7982293.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
BASE COUNT        27486 a        16407 c        20322 g        27312 t
ORIGIN      
        1 gaaaaacgtg gactggtttc gtgtttggat tattaccttg aacgacatgc gttaaaagtt
       61 agttgaaccg ccgtatgccg aacggcacgt acggtggtgt gagaggggct agagattatc
      121 ccctactcga tgtgcttttt gatatgagcc catgttttct caataggatt gtactcaggt
      181 gagtagggag gaagaggtaa aagtttacgc ccaaactctt cacataagag ttctagcttc
      241 cccattctat ggaatcttgc attatccata ataataaccg atggtgtggt taatgttggt
      301 aagagaaact tctgaaacca agcttcaaaa aagtcgctcg tcatcgtctc ttcgtaagtc
      361 attggagcga ttaactcacc atttgttaga cctgaaacca aagaaatcct ctgatatctt
      421 cttccagata ctttgcctct tcttaactga ccttttaatg agcgaccata ttctcgataa
      481 aaataagtat cgaatcctgt ttcgtcaatc taaacaggtg ctaggtgctt taaactatta
      541 aaattcttaa gaaataaggc tacttttctg ggtcttgttc atagtaggtg tggttctttt
      601 tttagagtgt agcccatagc tttgagcgca tagtggatgg tagttggatg acagccaaat
      661 tcagaagcta tttcagtcaa ataagcgtct ggattgtcag taagatagtt tttaagtcta
      721 tctctatcaa cttttcttgg ttttattcct tttacttggt ggtttagctc tcctgttttc
      781 tcttttagct ttaaccagcc ataaatggta ttacgtgaga tttggaaaac gtgtgatact
      841 tctgttatac tacctgttcg ctcacaataa gagagaactt ttttacgaaa atctattgaa
      901 tattccataa aagattatac cacattgtgt actatagtag attgaaacta gaatagtaca
      961 cctctgcttc taaaacattg ttagaaatcg atttgactgt cctgatcgat ttgtcatgtt
     1021 cttatttcat tttactatat ttttgtttcg cgggaagtct actaagatac ttaaagatgc
     1081 agatagtaaa aaaaatgtag acattaccgt aaaaaagtga tataatcgta tgatgttcaa
     1141 ggtataggtg ttaatcatga gtagacgttt taaaaaatca cgttcacaga gagtgaagcg
     1201 aagtgttaat atcgttttgc tgactattta tttattgtta gtttgttttt tattgttctt
     1261 aatctttaag tacaatatcc ttgcttttag atatcttaac ctagtggtaa ctgcgttagt
     1321 cctactagtt gccttggtag ggctactctt gattatctat aaaaaagctg aaaagtttac
     1381 tatttttctg ttggtgttct ctatccttgt cagctctgtg tcgctctttg cagtacagca
     1441 gtttgttgga ctgaccaatc gtttaaatgc gacttctaat tactcagaat attcaatcag
     1501 tgtcgctgtt ttagcagata gtgatatcga aaatgttacg caactgacga gtgtgacagc
     1561 accgactggg actgataatg aaaatattca aaaactacta gctgatatta agtcaagtca
     1621 gaataccgat ttgacggtca accagagttc gtcttacttg gcagcttaca agagtttgat
     1681 tgcaggggag actaaggcca ttgtcctaaa tagtgtcttt gaaaacatca tcgagtcaga
     1741 gtatccagac tacgcatcga agataaaaaa gatttatacc aagggattca ctaaaaaagt
     1801 agaagctcct aagacgtcta agaatcagtc tttcaatatc tatgttagtg gaattgacac
     1861 ctatggtcct attagttcgg tgtcgcgatc agatgtcaat atcctgatga ctgtcaatcg
     1921 agataccaag aaaatcctct tgaccacaac gccacgtgat gcctatgtac caatcgcaga
     1981 tggtggaaat aatcaaaaag ataaattaac ccatgcaggc atttatggag ttgattcgtc
     2041 cattcacacc ttagaaaatc tctatggagt ggatatcaat tactatgtgc gattgaactt
     2101 cacttctttc ttgaaaatga ttgacttatt gggaggggta gatgttcata atgatcaaga
     2161 gttttcagct ctacatggga agttccattt cccagtaggg aatgtccatc tagactctga
     2221 gcaggctcta ggttttgtac gtgaacgcta ctcactagcc gatggagacc gtgaccgtgg
     2281 tcgcaaccaa caaaaggtca ttgtagcaat tattcagaag ttaacttcta cagaggtttt
     2341 gaaaaactat agtagtattc ttcaaggatt gcaggattct cttcaaacaa atatgccgat
     2401 tgagactatg atagatttag tgaatactca gttggaaagt ggggggaatt ataaagtaaa
     2461 ttctcaagat ttaaaaggga caggtcggat ggatcttcct tcttatgcaa tgccagacag
     2521 taacctctat gtgatggaaa tagatgatag tagtttagct gtagttaaag cagctataca
     2581 ggatgtgatg gagggtagat gaaatgatag acatccattc gcatatcgtt tttgatgtag
     2641 atgacggtcc caagtcaaga gaggaaagca aggctctctt ggcagaatcc tacaggcagg
     2701 gggtgcgaac cattgtctct acctctcacc gtcgcaaggg catgtttgaa actccggaag
     2761 agaagatagc agaaaacttt cttcaggttc gggaaattgc aaaagaagtg gcagatgatt
     2821 tagtcattgc ttatggcgca gagatatact atactctgga tgctctagaa aagctagaaa
     2881 aaaaagaaat tcctaccctt aatgatagtc gttatgcctt gattgagttt agcatgcata
     2941 cttcctatcg tcagattcat acgggattga gcaatatttt gatgttggga atcacgccag
     3001 taattgctca tattgaacgt tatgatgctt tagagaataa cgaaaaacgt gttcgtgaac
     3061 tgattgatat ggggtgctat actcagataa atagttatca tgtttcaaaa cctaagttct
     3121 ttggtgaaaa atataaattc atgaaaaaga gagctcggta ttttttggaa cgtgatttag
     3181 ttcatgtagt tgcaagtgac atgcacaatt tagacagtag acctccatat atgcaacagg
     3241 catatgatat cattgctaag aaatatggag cgaaaaaagt aaaagaactt tttgtagata
     3301 atcccagaaa aattataatg gatcaattaa tttaggagaa aatatgaagg aacaaaacac
     3361 tttggaaatc gatgtattgc aactattcag agctttatgg aaaagaaagt tggtcatttt
     3421 attagtggca attataactt cttcagttgc ttttgcctac agtacttttg ttatcaaacc
     3481 tgagtttact agtatgactc ggatttatgt agttaaccgt aatcagggag agaagtctgg
     3541 tttaaccaat caagacttgc aggcaggatc atccttggtt aaagactatc gtgaaattat
     3601 cctatcgcag gatgttttgg aggaagttgt ttctgatttg aaactagatt tgacgccaaa
     3661 agatttggct aataaaatta aagtaacagt accagttgat acccgtattg tctctgtttc
     3721 agttagtgat cgagttcctg aagaggcaag ccgtatcgct aactctttga gagaagtagc
     3781 tgctcaaaaa attatcagta ttactcgtgt ttctgatgtg acaacactgg aggaggcaag
     3841 accggcgaca tcaccgtctt cgccaaatat taaacgcagt acactaattg gttttttggc
     3901 aggagtgatt ggaactagtg ttatagttct tattcttgaa cttttggaca ctcgtgtgaa
     3961 acgtccgaaa gatatcgaag atacactgca tatgacactt ttgggaattg taccaaactt
     4021 gaataagttg aaataggaga gaggaatgcc gacattagaa atagcacaaa aaaaactgga
     4081 gttcgttaag aaggcagaag aatattacaa tgccttgtgt acaaatatac agttgagcgg
     4141 agataaacta aaagtaattt ccgttacttc tgttaaccct gaggaaggaa aaacaactac
     4201 ttccgtaaat atagcaaggt cgtttgcgcg tgcaggctat aaaactcttt tgatcgatgg
     4261 cgatactcga aattcagtta tgtcaggatt ttttaaatct cgtgaaaaaa ttacagggct
     4321 aacggaattt ttatctggga cagctgattt atctcacggt ttatgtgata caaatattga
     4381 aaatttattt gtagttcaat cgggcactgt atcaccaaac cctacagcct tgttacaaag
     4441 taaaaatttt aatgatatga ttgaaacatt gcgtaaatat tttgattata tcattgttga
     4501 tacagcacct attggaattg ttattgatgc ggcaattatc actcaaaagt gtgatgcgtc
     4561 catcttggta acagcaacag gtgaggtgaa taaacgtgat gtccaaaaag cgaaacaaca
     4621 attagaacaa acagggaaac tgttcctagg agttgttttg aataaattag atatctcggt
     4681 tgataagtat ggagtttacg gtttctatgg aaattatggt aaaaaaataa tttaggaaag
     4741 attctatgga tgaaaaagga ttgaaaattt ttttggcagt attacagagt attattgtca
     4801 ttttattggt ttattttctt agctttgtta gagagacaga acttgaacgt tcttcgatgg
     4861 ttatactata ccttctccac ttttttgtat tctattttag ttcctatggt aacaattttt
     4921 ttaaaagagg gtacctagtt gagtttaata gtaccataag atatattttt ttctttgcaa
     4981 tagctataag tgtattaaac ttttttatag cggaacggtt tagtatctct agaagaggaa
     5041 tggtatactt cttaacttta gaaggaatat ccttatactt gttaaatttc ttagtaaaga
     5101 aatattggaa gcatgtgttt tttaatctaa aaaatagcaa gaaaatttta ctgttaacag
     5161 taacgaaaaa tatggaaaaa gttcttgata aattgctaga atctgatgaa ctttcatgga
     5221 aattggtagc agtaagtgtt ttggataaat ctgattttca acatgataaa atacctgtaa
     5281 ttgaaaagga aaaaattatt gaatttgcaa cgcatgaagt tgtggatgag gtgtttgtca
     5341 atcttccagg agagagctac gatattggag aaattatctc taggtttgag acaatgggga
     5401 tagatgtaac tgtaaatctt aaagcatttg ataagaattt gggtcgcaat aaacaaattc
     5461 atgagatggt aggattgaat gtagtcactt tctctacaaa tttttataaa actagtcatg
     5521 taattgctaa gcggattatt gatatcatgg gtgcattggt agggctgata ctatgtggtt
     5581 tagtcagtat tgtattggtt cctttgattc gaaaggatgg gggctctgct atttttgctc
     5641 agacgcgtat aggaaaaaat ggtcgtcagt tcacttttta taagtttcgc tctatgtgtg
     5701 tagatgccga ggcgaaaaaa agagaactca tggaacaaaa taccatgcag ggtggaatgt
     5761 ttaaggtgga cgatgatcct cgtatcacga aaattggtcg ttttatacgg aagactagct
     5821 tggacgagtt accacagttt tataatgttc taaagggaga tatgagtttg gttggcacac
     5881 ggccacctac agtggacgag tatgttcagt atacttcaga acagaaacgt cgactcagct
     5941 ttaaacctgg tattacaggt ttatggcagg ttagcggccg tagtaaaata accgattttg
     6001 acgatgttgt aaaattagat gtggcttata ttgataattg gacaatctgg aaagatattg
     6061 aaattttgct taaaactgtt aaagttgtat ttatgagaga tggagcgaag tgaggaatga
     6121 gggatagaat ccaactttta ggtgtaacaa ttgatttgct tacgatgaat gaaacgatag
     6181 atagtgtaga acaatatgta ttagaaaaaa gaccactaca cttgatgggg gtgaatgctg
     6241 ataaaattaa tcagtgtcat acagatgaga aaatcaaaaa aatcgttaat gagtcaggaa
     6301 tcattaatgc ggatggagca tcagttgttc ttgcaagtaa gtttttagga acgcctgttc
     6361 ctgaacgagt agcgggtatt gatttgatgc aatgtctttt agagttgtca aataaaaaag
     6421 gatattcagt ttactttttt ggagcaaaag aagaagtttt gcaagatatg ctcaaagtat
     6481 ttaagagaga ttatccaaat ttgatagtta ttggacacag aaatggctat ttttctgaag
     6541 aggatgaaca agctattcaa gaagatattc gtgaaaagaa ccctgatttt gtgtttattg
     6601 gaattacgtc tcctaaaaaa gaatatatta ttcaaaaatt tatggatagt ggcgtcaatt
     6661 cggtatttat gggagttggc ggtagttttg atgtcttgtc tggtcatatc caacgagcac
     6721 ctctatggat gcaaaagtca aatttagagt ggttattccg tgtagctaat gagcctaaac
     6781 gtctctttaa acgttatttt gtagggaata tttcattcat aggaaaagtt ttaaaagcaa
     6841 aaagaggtgt aaaatattga accagacaga gatgattcgc ttaattcaaa aagttgaatt
     6901 agatgctata aaagagttta aaaaaatctg tgaagagaat gatatagatt ttttcctccg
     6961 cggtggtagt gtacttggtg cggtcaaata cgacggcttt attccatggg atgatgatat
     7021 ggatatcgct gtccctcgtg aagcatacga caaacttcca agtgttttca aagatagaat
     7081 tatcgctggg aaatatcagg ttcttactta tcaatactgt gatacgttgc attgctactt
     7141 tcctcgacta ttccttttag aagatgaaag aaaacgtttg ggcttgccac gaaataccaa
     7201 tctaggattg catttgattg atatcattcc tttagatgga gcaccaaatc attcggtttt
     7261 aagaaagatt tactttggca aagtatactg gtatcgtttt ttagcaagtt taggaacaac
     7321 ttatgttggc gaccatgtgg atatgcattc cactaagcaa aaactaatta ttggtttctt
     7381 taaaaaacta ggatttgcaa aactatttcc tcaaaattct gtatacagac gcttggataa
     7441 tctctataga aagtatgatt ggaaaaagca gaagtatgcg gggactatca atgcttcttt
     7501 atttgctaaa gaagttatgc cagtagagat ttggggagaa ggagtagaga agccttttga
     7561 ggataccttc tttaaagttc caacggagta tgatcgctac ctgaaaagac tttacggaga
     7621 aaactatctt tacgaagagc ctagtgatga tgaaaagaaa tcgcatttag gaggacacta
     7681 atttgttttg ttatattatt ttgcattaca aagtcttaga agaaactatt tcttgtgtta
     7741 aatctataaa agaaggcaat tataatgcaa agcaaatcgt tattattgat aatttctcta
     7801 ataatggtac tggtgaaaaa ctacaagagc tttatgaatc agatttagaa attgatgttt
     7861 tgattaacca tgaaaatgct ggttttgctc gtggaaataa tgtggcttat caatttgcta
     7921 aggaaaagta taaccccgat ttcatggtta tcatgaataa cgatattgag atagaaacag
     7981 aaaattttga aaaaattgtg acagatatct atcgtgagga aaaattccat ttgctcgggc
     8041 cagatatctt ctcgactact taccaacttc accaaaaccc aaaacggttg acacattata
     8101 cttatggaga agttaaagct ctaaatgaaa aatttaaaaa agggagccaa gttagtctag
     8161 ctttaaaaat caaatgttgg ttgaaagcta gtaaagttct tcgaacagca atctatcaaa
     8221 atagacgtaa aaaaggatca gtagactata gaaaacaggt agaaaaccca attcttcatg
     8281 gttcttttat tgtatattcg agagatttta tcgaaaaaga ggagtatgct tttaacccta
     8341 acaccttctt ttactatgaa acagagatat tagattatga agctgaatta aaaggataca
     8401 agagaattta tacacctaaa attagagttt tgcaccatca aaatgttgca actaatcaag
     8461 tttacacgaa cttgttagaa aaaaccttgt tttcaaataa atgcaacttt aaatccacca
     8521 gttatttttt gaagttgatg aaagaaaacg aggatgttta aatgagttat ttatttttac
     8581 tttgccttac attattctta ttgactatat tctatttctt tgcttttatt caagatttaa
     8641 ttgctcctcc agtagttatg tctgtaatgt ttctaattag ttcagtattt gcactggtta
     8701 attcaaaaaa ctggaatatt gaatatagtg gaatagccta tattctcata attagtggta
     8761 ttattatatt ttcgattcct ttaatggcat taaaatcacc taattttaat actgaggtta
     8821 agattgctga tcgattaatt gatatccaat tttggaaaat tgctctaact attataattg
     8881 atctctttat tttgtatctt tacaggaagg aaatatacaa ccttgttctt agtaatggat
     8941 atacggggtc aaatattcag tggtttttta gaaatgcaac gagttatgaa ggtgaattga
     9001 cagtgcgaac ttttattcga gttctcattc gtgttattga cgtatctgct tatatttttg
     9061 gatatacttt tattaataat tttcttatct atcgccataa acgccctaaa gacatattac
     9121 ttttagtacc tttattaata tttatttcaa aaactttaat atcaggaggc cggcaagata
     9181 ttattaaaat tctgattgcc tatgtaatca tgatgtatat ccaacaaaaa cggaaagttg
     9241 gatggaatag agtcatatct cataaatata ttcaccttgg atttgttggt ttaatagcag
     9301 gtattccagc attttactac tctttgtttt tagccggtcg ttcaacgact aggacgctat
     9361 ttgagagtgt ttcgacctat ctaggaggct caattcagca ttttaatcag tatattgaaa
     9421 atccattaga tcctggtgaa gtttttggca gtgaaacatt ggtgcctata ttaaatatat
     9481 taggggaaat gggcctagtt aattatcgta gtacaattca tttagaattt cggacactag
     9541 gagttactgt aggaaatgtt tatacttttt ttagaagacc cttgcatgat tttggtctag
     9601 ttggtatgta tgtatttgtc tttgctgtag gtgctttttt tgctatttat tatttagttc
     9661 tgagaaagaa acaggttggt tttaatttgg atattcatac cattatttat tcttatgtct
     9721 tttattggat ttttttatca tcaatcgagc aatactcgtt cacaatgatt agtctatata
     9781 cacttgtatt tattgtgttg gtttacttta tggctatctt ttactggtgt acagatttta
     9841 aaagaggaaa actgattttt aaaatttctg actcaagtat caaattaaaa gaagaataac
     9901 agaatgtata ggagagggta gatgaatact aaaattaaaa atataataac tagtttttct
     9961 tatgttattt cttcaaatct gctcatagtt ttaacctcat cactagttgt tttgattttt
    10021 cctaaaataa tgggggtaac tgagtacagt tactggcaac tttatatttt ttatctgacc
    10081 tatatcggtt ttttccactt gggttggatt gatgggattt atctcaaata tggtggctta
    10141 gaatatacaa atttagatag aaaacagttt tattctcaga tgattctatt ttctagtttc
    10201 ttaatgctaa tctcgctggt attatttact ttgaacctaa taactgtaag ggatgaaaac
    10261 gcaagatata tttataatat ggctatcatt agcatgatag tcacaaactt aagaacactc
    10321 tatgtttata tcttgcagat gacaaatcgc ttgaaggata gttcagtcat tctaattagt
    10381 gatcgcgttt tatatgtact ccttttattc atgtttattg tatttggatg gcatgagtac
    10441 aaggtcatga tttgggctga tattctaggt cgaacatttt ctctcatgct ttccttctgg
    10501 atttgtaaag atattgtgtt tcagcctttg tcaaaattta tcttagattt caaggagtcc
    10561 cttgataata tccgtgttgg aatcaattta atgctatcta acattgcgag tagcatgatt
    10621 ataggcattg ttcgtatggg aattcaatgg aattggaata ttgaaacatt cgggaaagtg
    10681 tcattaactt tgagtatatc taatttatta atgactttta ttaatgccat tggattagtt
    10741 atctttcctt tgataaagcg aacaaagact gagaatttac ctaaaattta ttctaattta
    10801 agaaatgctt tgatgttggt tatgttcgca atcttgctct tctactatcc tttaaaattt
    10861 attcttgata tttggcttcc tgcttataag gatgccttag ttttcatggc cctaattttt
    10921 cctatgtcag tttatgaagg gaaaatggct ttggtgataa atacatattt aaaagcaatg
    10981 agaatggaaa aagacattct caaaattaat gctttggtta tgttaactag tatagtagtg
    11041 acattagtga ctactatact actaaataat ttggggctga cagttgtatc tatagttatt
    11101 ttacttgctt taagaagtat aatagctgaa ttaattttat ccaaaaaact gaagatatca
    11161 gtcaagcaag acattgcttt agagttactt atgacgatta tatttatttc ttcaagttgg
    11221 tatctctcta tttggattgc agtaataatt tatttattgg cgtatacttt atatttgtat
    11281 ttaaagcaca aagatatcag aatgtatata gaatacttta aaaatcataa aaaaatatca
    11341 taaaaattat atatcaatga aatggtagat tacatttcta cctttttatc catttaggag
    11401 gaaacgatga aaaagataat gctagttttc ggtacacgtc cagaagcaat aaaaatgtgt
    11461 tcattagtca atgagttgag aaaacaggaa gatatgaaaa cagttgtttg tgtaactggt
    11521 caacacaagg agatggttag ccctgtttta gatttatttg gagttcaacc agactatgat
    11581 ttagaaatta tgaaggctaa tcaaaacttg ttctctataa caataagtat tttagaaaaa
    11641 ataaaacctg tgttagaaaa agaacaacca gatattgttt tggttcacgg tgatactact
    11701 acgacatatg cagcagcttt agcggcattt tatctaggaa ttaaagttgg tcatgttgaa
    11761 gctggattac gaacttacaa cctgcaaagt cctttcccag aagagttcaa cagacaatcg
    11821 acttctatta ttgcaaatta tcattttgct cctacagaat tagctaaaga aaatctaata
    11881 aaagagggta gaaataatat ctatgttact ggaaatacag tgattgatgc acttacaact
    11941 acagtacaaa aggattatac acaccccgat ctagatttaa acgatggaaa tcgcctcatc
    12001 ttattgactg ctcatagacg cgaaaatctc ggagaaccta tgagacatat gtttagagct
    12061 gttaaacgag ttttaaatga atatgacgat gttaaagtaa tttatccaat tcataaaaat
    12121 ccattggtac gggaaacagc tacagaaatt tttggagata cagaacgtat tcagatcatt
    12181 gaacctttag atgttcttga ttttcacaac tttatgaatc atagttatat gattctaact
    12241 gactcaggag gggttcaaga agaggcacct tcgttaggaa agcctgtatt ggtcatgcga
    12301 gatacgacag aaagacctga aggagtagct gctggaacgt tgaaattggt tgggactgat
    12361 gaggagacta tttatcaaaa ctttaagatg cttttagacg atcccgaaga atataaaaaa
    12421 atgagtcaag ctagtaatcc ttatggaaat ggtgatgcta gtaaacagat tgttcgaatt
    12481 ttacgtggaa tttgagtgtg ttcagataaa gtaatataga aaggtaccct actatgaaag
    12541 gtattattct agcaggtggt tcggggacac gtttatatcc tttgactcgc gctgcatcaa
    12601 aacaacttat gccggtttat gataaaccga tgatttacta cccactttca acattgatgt
    12661 tggctgggat tagggatatt ttgattattt ccactccaca ggatttacat cgattccaag
    12721 agcttcttca agacggatct gagtttgggc tcaaactttc ttatgcagag caaccaagtc
    12781 cagatggttt ggcacaagcc tttatcattg gggaagagtt tatttctgat gatagcgttg
    12841 cgctgatctt aggtgacaat atctatcacg gttctgggct ttccaagatg ctacaaaagg
    12901 cagcgagtaa ggagtcggga gcaactgttt ttggctacca tgtcaaggat ccagagcgct
    12961 ttggtgtggt tgagtttgat caggatatga aggctatttc tattgaagaa aagccagagc
    13021 aacctcgttc aaactatgca gttacaggtc tctatttcta tgataatgat gtagtagaga
    13081 ttgccaagag tattaaacca agtcctcgtg gtgaactgga aattacagat gtaaacaagg
    13141 cttacctaga tcgtggtgat ttgtctgttg agcttatggg acgtggcttt gcttggctgg
    13201 atactggaac tcatgaaagt ttactagagg cttcacagta catcgaaaca gtgcaacgga
    13261 tgcaaaatgt tcaggtagca aacttagaag aaattgctta ccgtatgggc tatatcagtc
    13321 gagaagatgt attggcctta gcccaaccac ttaagaaaaa tgaatacgga cagtatctgc
    13381 tccgtttgat tggagaagca tagatgacag ataatttttt cggtaagacg cttgcggcac
    13441 gcaaggttga agctattcca ggcatgttgg agtttgatat ccccgttcat ggagataatc
    13501 gtggctggtt taaagaaaat ttccaaaagg aaaaaatgct tccacttgga tttccagagt
    13561 ctttctttgc agaaggaaaa ttgcaaaaca atgtatcctt ctcacgtaaa aatgtccttc
    13621 gaggtctcca cgcagagcct tgggataagt acatctctgt agcagatgga gggaaagttc
    13681 tgggttcttg ggttgatcta cgcgagggtg aaacctttgg gaatacctat cagacagtga
    13741 ttgatgcgag taagggaatc tttgttcctc gaggcgtagc taatggcttt caagttttat
    13801 cagatacagt gtcatatagc tatctggtca atgattactg ggctcttgaa ctcaaaccca
    13861 agtatgcctt tgtgaactac gctgatccaa gccttggtat tgaatgggaa aatattgcag
    13921 aagcagaggt ttcagaagca gacaaacatc atccactact taaggatgta aaacctttga
    13981 aaaaagaaga tttggaataa ggaaagaata tgactgaata caaaaatatt atcgtgacag
    14041 gtggagctgg ctttatcggt tctaactttg tccattatgt ttacgagaac tttccagatg
    14101 ttcatgtgac agtcctagat aagttgactt atgctggaaa ccgcgcgaat attgaggaaa
    14161 ttttaggtaa tcgtgttgag ttagttgttg gtgacattgc tgatgcggag ttggtagaca
    14221 agttggctgc tcaagcagat gctatcgttc attatgcagc ggaaagccac aatgataatt
    14281 cgctcaatga tccatcgcca tttattcata ctaacttcat cggaacctat actcttttag
    14341 aagctgctcg taagtatgat attcgcttcc accatgtatc gacagatgaa gtttatgggg
    14401 atctcccttt acgcgaagat ttgccaggtc atggtgaagg accaggtgag aaatttactg
    14461 ctgaaacaaa atacaaccca agctctccgt actcatcaac caaggcagcc tcagatttga
    14521 ttgtcaaagc ctgggtgcgt tcttttggag tcaaggcaac gatttccaac tgttcaaata
    14581 actacggtcc ttatcaacat atcgaaaaat tcatcccacg tcagattact aacatcctaa
    14641 gtggtatcaa gccaaaactt tacggtgaag gtaagaacgt tcgtgattgg attcatacca
    14701 atgaccattc ttcaggagtt tggacaatct tgacaaaagg gcaaatcggt gaaacctact
    14761 tgattggggc tgatggtgag aagaacaata aggaagtttt ggaacttatc cttaaggaaa
    14821 tgggacaagc tgcggatgcc tatgatcatg tgactgaccg tgcaggacat gaccttcgct
    14881 atgcgattga tgctagcaag ctccgtgatg agttggggtg gaaacctgaa tttaccaact
    14941 ttgaagctgg gctcaaggca acaatcaagt ggtatacaga taaccaagaa tggtggaaag
    15001 cagagaaaga agctgttgaa gccaattatg ctaagactca ggagattatt acagtataaa
    15061 aagcaggaaa tagctgcttt ttattgctat attgggaaga gttacatatt agaaaggtct
    15121 agagatgatt ttaattacag gggcaaatgg ccaattagga acggaacttc gctatttatt
    15181 ggatgaacgt aatgaagaat acgtggcagt agatgtggct gagatggaca ttaccgatgc
    15241 agaaatggtt gagaaagttt ttgaagaggt gaaaccgact ttagtctacc actgtgcagc
    15301 ctacaccgct gttgatgcag cagaggatga aggaaaagag ttggacttcg ccatcaatgt
    15361 gacggggaca aaaaatgtcg caagagcatc tgaaaagcat ggtgcaactc tagtttatat
    15421 ttctacggac tatgtctttg acggtaagaa accagttgga caagagtggg aagttgatga
    15481 ccgaccagat ccacagacag aatatggacg cactaagcgt atgggggaag agttagttga
    15541 gaagcatgtg tctaatttct atattatccg tactgcctgg gtatttggaa attatggcaa
    15601 aaacttcgtt tttaccatgc aaaatcttgc gaaaactcat aagactttaa cagttgtaaa
    15661 tgaccagtac ggtcgtccga cttggactcg taccttggct gagttcatga cctacctagc
    15721 tgaaaatcgt aaggaatttg gttattatca tttgtcaaat gatgcgacag aagacacaac
    15781 atggtatgat tttgcagttg aaattttgaa agatacagat gtcgaagtca agccagtaga
    15841 ttccagtcaa tttccagcca aagctaaacg tccgctaaac tcaacgatga gcctggccaa
    15901 agccaaagct actggatttg ttattccaac ttggcaagat gcattgcaag aattttacaa
    15961 acaagaagtg agataagtag tagaatgatt ttctagtcta ataaaagagg cagataatga
    16021 actccaaagg agcttaagat gtacgattat cttgttgttg gtgctggtct ctttggcgca
    16081 tagctttggc tcagtttcta ttatcgctca caccatccat cagaagttta atctgaaggt
    16141 acccaattat cgccaagaag aagattgggc taggatgggt ttaccaatca cacgtaagga
    16201 aatctctaat tggcatatca aggcaagtca atactattta gagtcccttt ataacctttt
    16261 acgagaaaag ttgttagaac aacctcttct tcatgcggat gaaacctctt atcgggtctt
    16321 agaaagtgat agtcagctga cctactattg gacctttttg tctgggaaag ctgagaatca
    16381 agtaatcacg ctttaccacc atgatcagtg tcggagtggt tcggtagtgc aagaattcct
    16441 aggagattat tctggctatg tgcattgtga tatgttgcgg cagtaactta ggactttagt
    16501 cctctagttc tgtctatgcg atagcagtcc aaggtttagg agcaaggcga cgctaagctt
    16561 ggtaaactgc gaaccgctag aagcttatcg tcaactggaa gaagctgaac ttgttggatg
    16621 ttgggcgcat gtgagaagga aattttttga agtgcccccc aagcaagcag ataaatcatc
    16681 cttaggagct aaaggtttag cttattgtga tcagttattt tccttggaaa gagactggga
    16741 ggctttgcca gctgatgaac gactacagaa acgtcaagaa catctccaac ccctaatgga
    16801 agacttcttt gcttagtgcc gtcgtcagtc agttttatcg ggttcaaaac taggaagggc
    16861 aattgaatac agcctcaagt atgaagaaac ctttaagacc attttaaaag acggacatct
    16921 ggtcctttcc aataatctag ctgaacgcgc cattaaatca ttggttatgg gacggagtaa
    16981 aagagtccag tggactcttt tagcctaagc taaattttaa aaagcgaggg tggttatttt
    17041 ctcaaagttt tgaaggagct aaagcaagag ctattattat gagcttattg gaaacagcta
    17101 aacgtcatca actaaatagt gagaaatatc tatcctatct tctagaatgt cttccaaacg
    17161 aggaaactct cgtaaacaaa gaggttttag aggcctattt accatggact aaagttgtac
    17221 aagaaaagtg caaataagaa atctccagat taggaactat ccgtgagttc actaatctgg
    17281 agatttttca atagacctcg ttattgggcg gttacgatat tcatattttt tgcaaagatg
    17341 ttgtttgaaa aataattttc aaaaattctg aaaattctgt tgacatcttt ctgaaaagag
    17401 tctataatgg agagaaagtt ttaaaggaga aaatgatgaa aagttcaaaa ctacttgccc
    17461 ttgcgggcgt gacattattg gcggcgacta ctttagctgc atgctctgga tcaggttcaa
    17521 gcactaaagg tgagaagaca ttctcataca tttatgagac agaccctgat aacctcaact
    17581 atttgacaac tgctaaggct gcgacagcaa atattaccag taacgtggtt gatggtttgc
    17641 tagaaaatga tcgctacggg aactttgtgc cgtctatggc tgaggattgg tctgtatcca
    17701 aggatggatt gacttacact tatactatcc gtaaggatgc aaaatggtat acttctgaag
    17761 gtgaagaata cgcggcagtc aaagctcaag actttgtaac aggactaaaa tatgctgctg
    17821 ataaaaaatc agatgctctt taccttgttc aagaatcaat caaaggattg gatggctatg
    17881 taaaagggga aatcaaagat ttctcacaag taggaattaa ggctctggat gaacagacag
    17941 ttcagtacac tttgaacaaa ccagaaagct tctggaattc taagacaaca atgggagtaa
    18001 tggctccagt taatgaagag tttttgaatt caaaagggga tgattttgcc aaagctacgg
    18061 atccaaatag tctcttgtat aacggtcctt atttgttgaa atccattgtg accaaatctt
    18121 ctgttgaatt tgcgaaaaat ccgaactact gggataagga caatgtgcat attgacaaag
    18181 ttaaattgtc attctgggat ggtcaagata ccagcaaacc tgcagaaaac tttaaagatg
    18241 gtagccttac agcagctcgt ctctatccaa caagtgcaag tttcgcagag cttgagaaga
    18301 gtatgaagga caatattgtc tatactcaac aagactctat tacgtattta gttggtacaa
    18361 atattgaccg tcagtcctat aaatacacat ctaagaccag cgacgaacaa aaggcatcga
    18421 ctaaaaaggc tctcttaaac aaggatttcc gtcaggctat tgcctttggt tttgatcgta
    18481 cagcctatgc ctctcagttg aatggacaaa ctggagcaag caaaatctta cgtaatatct
    18541 ttgttccacc aacatttgtt caagcagatg gtaaaaactt tggcgatatg gtcaaagaga
    18601 aattggttac ttatggggat gaatggaagg atgttaatct tgcagattct caggatggtc
    18661 tttacaatcc agaaaaagcc aaggctgaat ttgctaaagc taaatcagcc ttacaagcag
    18721 aaggtgtgac tttcccaatt catttggata tgccagttga ccagacagca actacaaaag
    18781 ttcagcgcgt ccaatctatg aaacaatcct tggaagcaac tttaggagct gataatgtca
    18841 ttattgatat ccaacaacta caaaaagacg aagtaaacaa tattacatat tttgctgaaa
    18901 atgctgctgg cgaagactgg gatttatcag ataatgtcgg ttggggtcca gactttgccg
    18961 atccatcaac ctaccttgat atcatcaaac catctgtagg agaaagtact aaaacatatt
    19021 tagggtttga ctcaggggaa gataatgtag ctgctaaaaa agtaggtcta tatgactacg
    19081 aaaaattggt tactgaggct ggtgatgagg ctacagatgt tgctaaacgc tatgataaat
    19141 acgctgcagc ccaagcttgg ttgacagata gtgctttgat tattccaact acatctcgta
    19201 cagggcgtcc aatcttgtct aagatggtac catttacaat accatttgca ttgtcaggaa
    19261 ataaaggtac aagtgaacca atcttatata aatacttgga acttcaagac aaggcagtca
    19321 ctgtagatga ataccaaaaa gctcaggaaa aatggatgaa agaaaaagaa gagtctaata
    19381 aaaaggctca agaagatctc gcaaaacatg tgaaataact gttgcaaaat ataagaaagg
    19441 atttagtatt tctcttgaat gctgaatcct tttttacatt tgtaaagaaa gattctaaat
    19501 gtactgaccc ccaaaagttg gagcctcttt ttgtcagaat agagaaaatt tttgttaatt
    19561 ttacttgttt cctattgctt tctcagttat tatttgttat attaaaagta taattatttt
    19621 tatttatcag ggttaagcat tgcattttca gaggaaggag tattttttaa aaaggaaatg
    19681 taaacgcttg ctcaaaaatg aaaggattta gaagtttatg aataaaggat tatttgaaaa
    19741 acgttgtaaa tatagtattc ggaaattttc attaggtgtt gcttctgtta tgattggagc
    19801 tgcattcttt gggacaagtc cggttcttgc agatagcgtg cagtctggtt ccacggcgaa
    19861 cttaccagct gatttagcta ctgctcttgc aacagcaaaa gagaatgatg ggcgtgattt
    19921 tgaagcgcct aaggtgggag aagaccaagg ttctccagaa gttacagatg gacctaagac
    19981 agaagaagaa ctattagcac ttgaaaaaga aaaaccggct gaagaaaaac caaaagagga
    20041 taaacctgca gctgctaaac ctgaaacccc taagacggta acccctaaga cggtaactcc
    20101 tgaatggcaa acggtagaga aaaaagaaca acagggaaca gtcactatcc gagaagaaaa
    20161 aggtgtccgc tacaaccaac tatcctcaac tgctcaaaat gataacgcag gcaaaccagc
    20221 cctgtttgaa aagaagggct tgaccgttga tgccaatgga aatgcaactg ttgatttaac
    20281 cttcaaagaa gattctgaaa agggcaaatc acgctttggt gtcttcttga aatttaaaga
    20341 taccaataat aatgtttttg tcggttatga caaggatggc tggttctggg aatacaaaac
    20401 tccaggtaat agcacatggt ataaaggcaa ccgtgttgct gctcctgaaa caggatcaat
    20461 aaaccgtctt tctatcactc tcaagtcaga cggtcagcta aatgccagca ataacgatgt
    20521 caatctcttt gacacagtga ctctaccagc tgcggtcaat gaccatctta aaaatgagaa
    20581 gaagattctt ctcaaggcgg gctcttatga cgatgagcga acagttgtta gcgttaaaac
    20641 ggataaccaa gagggggtaa aaacagagga tacccctgct gaaaaagaaa caggtcctga
    20701 agttgatgat agcaaggtga cttatgacac gattcagtct aaggtcctca aagcagtgat
    20761 tgaccaagcc ttccctcgtg tcaaggaata caccttgaat ggacatactt tgccaggaca
    20821 ggtgcaacag ttcaaccaag tctttatcaa taaccaccga atcacccctg aagtcactta
    20881 taagaaaatc aatgagacaa cagcagagta cttgatgaag cttcgcgatg atgctcactt
    20941 aatcaatgcg gaaatgacag tacgcttgca agttgtggac aatcaattgc actttgatgt
    21001 gaccaagatt gtcaaccaca atcaagtcac tccaggtcaa aagatcgatg acgaaagaaa
    21061 actactttct tctattagtt tcctcggcaa tgctttagtc tctgtttcta gtgatcaaac
    21121 tggtgctaag tttgatgggg caaccatgtc aaacaataca catgtcagcg gagatgatca
    21181 tatcgatgta accaatccaa tgaaggattt ggctaagggt tacatgtatg gatttgtttc
    21241 tacagataag cttgctgctg gtgtttggag taactctcaa aacagctatg gtggtggttc
    21301 gaatgactgg actcgtttga cagcttataa agaaacagtc ggaaatgcca actatgtagg
    21361 aatccacagc tctgaatggc aatgggaaaa agcttataag ggcattgttt tcccagaata
    21421 cacgaaggaa cttccaagtg ctaaggttgt tatcactgaa gatgccaatg cagacaagaa
    21481 agttgattgg caagatggtg ccattgctta tcgtagcatt atgaacaacc ctcaaggttg
    21541 ggaaaaagtt aaggatatca cagcttaccg tatcgcgatg aactttggtt ctcaagcaca
    21601 aaatccattc cttatgacct tggatggtat caagaaaatc aatctccaca cagatggtct
    21661 tgggcaaggt gttctcctta aaggatatgg tagtgaaggt catgactctg gtcacttgaa
    21721 ctatgctgat attggtaagc gtatcggtgg tgtcgaagac ttcaagaccc taattgagaa
    21781 ggctaagaaa tatggagctc atctaggtat ccatgttaac gcttcagaaa cttatcctga
    21841 gtctaaatat ttcaatgaaa aaattctccg taagaatcca gatggaagct atagctatgg
    21901 ttggaactgg ctagatcaag gtatcaacat tgatgctgcc tatgacctag ctcatggtcg
    21961 tttggcacgt tgggaagatt tgaagaaaaa acttggtgac ggtctcgact ttatctatgt
    22021 ggacgtttgg ggtaatggtc aatcaggtga taacggtgcc tgggctaccc acgttcttgc
    22081 taaagaaatt aacaaacaag gctggcgctt tgcgatcgag tggggccatg gtggtgagta
    22141 cgactctacc ttccatcact gggcagctga cttgacctac ggtggctaca ccaataaagg
    22201 tatcaacagt gccatcaccc gctttatccg taaccaccaa aaagatgctt gggtagggga
    22261 ctacagaagt tatggtggtg cagccaacta tccactgcta ggtggctaca gcatgaaaga
    22321 ctttgaaggc tggcaaggaa gaagtgacta caatggctat gtaactaact tatttgccca
    22381 tgacgtcatg accaagtact tccaacactt cactgtaagt aaatgggaaa atggtacacc
    22441 ggtgactatg accgataacg gtagcaccta taaatggact ccagaaatgc gagtggaatt
    22501 ggtagatgct aacaataata aagtagttgt aactcgtaag tcaaatgatg tcaatagtcc
    22561 acaatatcgc aaacgtacag taacgctcaa cggacgtgtc atccaagatg gttcagctta
    22621 cttgactcct tggaactggg atgcaaatgg taagaaactt tctactgata aggaaaagat
    22681 gtactacttc aatacgcagg ccggtgcaac aacttggacc cttccaagtg attgggcaaa
    22741 gagcaaggtt tacctttaca agctaactga ccaaggtaag acagaagagc aagaactaac
    22801 tgtaaaagat ggtaaaatta ccctagatct tctagcaaat caaccatacg ttctctatcg
    22861 ttcgaaacaa accaatcctg aaatgtcatg gagtgaaggc atgcacatct atgatcaagg
    22921 atttaatagc ggtaccttga aacactggac catttcaggc gatgcttcta aggcagaaat
    22981 tgtcaagtct caaggggcaa acgatatgct tcgtattcaa ggaaacaaag aaaaagttag
    23041 tctcactcag aaattaactg gtttgaaacc aaataccaag tatgccgttt atgtcggtgt
    23101 cgataaccgt agtaatgcca aggcgagcat cactgtaaac actggtgaaa aagaagtgac
    23161 tacttatacc aataagtctc tcgctctcaa ctatgttaag gcctacgccc acaatacacg
    23221 tcgtgacaat gctacagttg acaatacaag ttacttccaa aacatgtacg ccttctttac
    23281 aactggatcg gacgtatcaa atgttactct tactttaagt cgcgaagctg gtgatcaagc
    23341 aacttacttt gatgaaattc gtacctttga aaacaattca agcatgtacg gagaaaacca
    23401 tgatacaggt aaaggcacct tcaaacaaga ctttgaaaat gttgctcagg gtatcttccc
    23461 attcgtagtg ggtggtgtcg aaggtgttga agacaaccgc actcacttgt ctgaaaagca
    23521 tgatccatat acacaacgtg gttggaacgg taagaaagtt gatgatgtta tcgatggaaa
    23581 ttggtcactc aagaccaatg gactggttag ccgtcgtaac ttggtttacc aaaccatccc
    23641 acaaaacttc cgctttgaag ctggtaagac ctaccgtgta acctttgaat acgaagcagg
    23701 ttcagacaat acctatgctt ttgtagtcgg taagggagaa ttccagtcag gtcgtcgtgg
    23761 cactcaagca agcaacttag aaatgcatga attgccaaat acttggacag attccaagaa
    23821 agccaagaag gcaaccttcc tcgtgacagg tgcagaaaca ggagatactt gggtaggtat
    23881 ctactcaact ggaaatgcaa gtaatactcg tggtgattct ggtggaaatg ccaacttccg
    23941 tggttataac gacttcatga tggataatct tcaaatcgaa gaaattaccc taacaggtaa
    24001 gatgttgaca gaaaatgctc tgaagaacta cttgccaacg gttgccatga ctaactacac
    24061 caaagagtct atggatgctt tgaaagaggc ggtctttaac ctcagtcagg ccgatgatga
    24121 tatcagtgtg gaagaagcgc gtgcagagat tgccaagatt gaagctttga agaatgcttt
    24181 ggttcagaag aaaacggctt tggtagcaga tgactttgca agtcttacag ctcctgctca
    24241 ggctcaagaa ggtcttgcaa atgcctttga tggcaatgtg tctagtctat ggcatacatc
    24301 atggggcgga ggagatgtag gcaagcctgc aaccatggtc ttgaaagaac caactgaaat
    24361 cactggactt cgttatgttc cacgtggatc aggttcaaat ggtaacttgc gagatgtgaa
    24421 acttgttgtg acagatgagt ctggcaagga gcataccttt actgcaactg attggccaaa
    24481 taacaacaaa ccaaaagata ttgactttgg taagacaatc aaggctaaga aaattgtcct
    24541 tactggtacc aagacatacg gagatggtgg agataaatac caatctgcag cggaacttat
    24601 ctttactcgt ccacaggtag cagaaacacc tcttgacttg tcaggctatg aagcagcttt
    24661 ggttaaggct cagaaattaa cagacaaaga caatcaagag gaagtagcta gcgttcaggc
    24721 aagcatgaaa tatgcgacgg ataaccatct cttgacggaa agaatggtgg aatactttgc
    24781 agattatctc aaccaattaa aagattctgc tacgaaacca gatgctccaa ctgtagagaa
    24841 acctgagttt aaacttagat ctttagcttc cgagcaaggt aagacgccag attataagca
    24901 agaaatagct agaccagaaa cacctgaaca aatcttgcca gcaacaggtg agagtcaatc
    24961 tgacacagcc ctcttcctag caagtgttag cctagcccta tctgctctct ttgtagtaaa
    25021 aacgaagaaa gactagtatt tagtaaaacc tcttaacaag attacggaag cagtctctat
    25081 cttttccaat gaggtttata gtacagaaaa agcctgagaa gatatcttct caggcttttg
    25141 ttaagcacat aaatacaata gtgctatgaa aaaatcaccc agaaaaatct ggatgataaa
    25201 tgttatggtt gtgctggttg aggattctga ttttgttgat caggggttgt atttgattgt
    25261 tgcgtattat tgttaggatt ggtagtcgta ctattatttg tgcttggagt ggttgagcta
    25321 gactgtgaag ttgaactatc tgatgatgag cttgaacttt cagttgatgg gggttgttgt
    25381 ggagcaggtg agctccacgt agaacgagca ccatttttaa atacgaattc tccatttctg
    25441 tagagcccct ctggtatatt ccaatcctct ggattgcttc cttcagacag gtaggtcatc
    25501 atagagcggt aaactttggc agcgaccgta aggccattgc ctacaagtgg tgtcagacgg
    25561 ttagaatagc ctgtccatac agccattgaa tatttacgcg tatagccagc aaatagttca
    25621 tcaggtgcta caaattgaga ggtcttgatg tggttttcaa tttcctcgtc tgtatagtta
    25681 gaggttcctg ttttaccagc ctgagggagc caagcaagat aggcatttcg tccagttcca
    25741 taactcaaga ctgttttcat catgtcggtc atcatatagg ctgtcgtttc cttcatggca
    25801 cgagttccga cattagagaa ctctttttca ctcccatcac taaagacgac tttatggata
    25861 tacattggtt tatagtaagt tccaccattt gcaaaggcag cgtaagcagc agccatcttt
    25921 tcactacttg ctccatattt tttgtctgat tcggttgtgt tacttgaaat ggcatttgag
    25981 tagtgaatac ttgggtagtc gattccgaga ccatttagga aagtcttggc gcggttgagt
    26041 ccgaccttgt ttagagtttc cacggctggg acgtttcgcg attgttgcag ggcgtattgc
    26101 aaggtgatgt tgccaaagta gcccctatcc cagttataaa caggggtatt tgtcccaggg
    26161 tagttatagg gctcatcgtg aacgatagta gcagttgaat cgtagacacc gtactccaag
    26221 gcaggagcat agtctgtgat cggtttcata gttgatcccc agtcgcggtt tgtttctact
    26281 gcttggttaa ttccgaagga aacattactt gactgatggc gtgctcctag ctgggcaatg
    26341 actttaccgt tagaaacatc aacaatggta gaagcgactt gcaattcatc gtctggatag
    26401 gcaacgtatt cgtctgtatt gtaaatatcc cacagatgtt tttgagcttc ttggtctaca
    26461 tttgtgtaga catccatccc agttgtgagc aggttatagc ctgtttcttc ttccacttga
    26521 ttgatgactt ccttgaggta attatccatg taagcagggt aattacttgc tgatttgaga
    26581 ctttgtagtc catcagtaat tggtgtattg actgctttct catactgttc agcagagatg
    26641 tagccttgat ttttcatttc agataagacc aagtttcggc ggtcttgggc tgcttctgga
    26701 tgtgaatagg ggtcatattg gtttggtgcc tgaggcattc cagccagcaa ggctaactga
    26761 ggtaaactta aattattgag gtctttacca tagtagtttt gagctgctgt ctgcattcca
    26821 tagttcccat tagacatgta gaccttattt atatagtagg tcaagatttc ttgcttggtt
    26881 gctttttgtt ctaactgaat cgctaaccaa gcttcctgaa ccttacgaga aatagtctgg
    26941 tcggaagtcg aagttgaaaa gtaagtcaac ttaatcaact gttgggtgag agttgatcca
    27001 ccttggaggg aattgctttg cagattgcgc aagaaagctc ccaggatacg gatggtatca
    27061 atccccctgt ggtcgaagaa gcgatggtct tcgatagaaa cgattgcctt aaccaaatct
    27121 gtgggaatat cattagcttg ggcattgacg cggcgttcag aacccaagtc agcaatgagt
    27181 tgatttttat tgtcgtagat tttactagaa gttgttgcaa ctagtttact ctcggatagg
    27241 ctaggagcct tgctaacgta gtagaaaaaa actcctccgc ctaagacaat ggctgcgata
    27301 accaagctta agaagctaat gctcagatac ttgattaggc gcagaatcgt tggtttgttc
    27361 atcttgtttt accacctaat aaatgttctt tgataacatt gagataagga atttgaggga
    27421 aggcaccagc cttgatttca tatccatatt ctcgaatata ttcaagtggc attgattttt
    27481 gtcccttatc ttgatgatag aagcgaatca aatcgaatgc cggcaataag taggtttctt
    27541 gctgagaaga aaagtgaaga aggacaaagc agattccttg ttgggcaagg acttgttcca
    27601 tatgctgaat ctgatgtgga tgaaaatttt tcatcggaat cgcacgtttt tgttttgttt
    27661 ccttgacttc aaagtcgatg taatatccat tataaacgcc agaatagtcc gtcgttgaag
    27721 cttgtcgaaa ataggcttca acaatcttgg cacgacttcg ttgtggatag tccacttgta
    27781 cgatttgaat aggagttggt ttcttatgta taacagccaa gccctgagac aaatagtagt
    27841 cgttggtagc attgatcatc ttttcaaaag acattcctcg atttgcgaaa tttttgggtt
    27901 gagaaagaga tgtttgtctt ttttgtgatg aaactttatg tggatagttg accataattc
    27961 tccttattgg tacaataaca tcactttatt ataccataat ttgcacagaa agggttaaaa
    28021 atggctacag ctttggtttt aggctattct acttttgatt tgggtttatt ttctgataag
    28081 gatcctcgtc ttaaattgat taaaaaagcg atacgtaaag atttagaggc tatggcagca
    28141 gatggggtgt cttggcttgt gtttacagga agtttgggtt ttgaatattg ggttttagag
    28201 gttgctcagg agatgaaaac cgaatacggt ttccagttgg ccaccatttt tgcttttgaa
    28261 acccatgggg aaaattggaa tgaaggtaat cagatgaaac tgagtcgctt taagcaggta
    28321 gactttgtca aatatgctta tcctcgctat gaacacaagg gacagttaag agattaccag
    28381 cagtttttgc tggaaaacac gactagttcc tatctttttt atgatgaaga aaatgaaaca
    28441 aaactagctt atttttacca aaagatgaaa aatcaagagg actattttat aaagagatta
    28501 acatttgatc aattaaatga acttgctgaa aatttttccg aaaattgaag ctttgacctt
    28561 gatttttgtt tgcctttttt tatataataa tactagcaag cgagaatgga gagagacatg
    28621 gcaagtatta ttttttcagc gaaagatatt tttgaacaag agtttggacg tgaagtccgt
    28681 ggctataata aagtagaagt tgacgagttt ttagacgatg tcatcaagga ctatgaaacc
    28741 tatgctgcct tggtcaagtc acttcgtcag gaaattgcgg atttgaagga agaattaact
    28801 cgtaaaccga aaccttcacc agttcaagca gaacctcttg aagcggcaat tacaagttct
    28861 atgacgaatt ttgatatttt gaaacgcctg aatagattgg aaaaagaagt ttttggtaaa
    28921 caaattttag ataactcaga tttttaagta gttatttgag atgtgcaatt tttggataat
    28981 cgcgtgagga gaattgcttc tcatgaggaa agtccatgct agcacaggct gtgatgcctg
    29041 tagtgtttgt gctaggcgaa accataagcc tagggacgag aaatcgttac ggcagttgaa
    29101 atggctaagt ccttggatag gccagagtag gcttgaaagt gccacagtga cggagtcttt
    29161 ctggaaacag agagagtgga acgcggtaaa cccctcaagc tagcaaccca aattttggtc
    29221 ggggcatgga gtacgcggaa acgaacgtag tattctgact gctatcagct agagctgtta
    29281 gtggtagaca gatgattatc gaaggaagtg gtcctagtca cttctggaac aaaacatggc
    29341 ttatagaaaa ttgcatatag gttggggctg agaaattttc tcaacctcat tttttaaagt
    29401 ggacatatag aaaggtcttg caagactgta acatgaaaaa agaatttaat ttaattgcaa
    29461 ctgtggcagc agggcttgag gctgtcgttg gtcgtgaagt gcgagagttg ggctacgatt
    29521 gtcaggttga aaatggacgt gttcgttttc aaggagacgt gagagctatt atcgaaacca
    29581 acctttggct tcgggcagca gatcgtatca aaattatcgt aggaacgttc ccagctaaga
    29641 cttttgaaga gctatttcag ggagttttcg ctttagattg ggaaaattat ttaccacttg
    29701 gagctcggtt cccgatttca aaagctaaat gtgttaagtc caaacttcac aatgagccca
    29761 gtgttcaggc tatttctaag aaagctgttg tcaagaaatt gcagaaacac tatgctcgcc
    29821 cagaaggggt tcctctgatg gagaatggcc cagagtttaa gattgaggtc tctattctca
    29881 aagatgtggc aactgtcatg attgatacga ccgggtctag cctctttaaa cgtggttatc
    29941 gtaccgaaaa aggtggcgct cctatcaagg aaaatatggc agcagccatt ttacaacttt
    30001 ctaactggta tccagacaag cctttgattg atccgacctg tggttcgggg actttctgta
    30061 ttgaggcagt tatgattgct agaaagatgg cgccaggtct tcgtcgctct tttgcatttg
    30121 aggaatggaa ctggatcagc gatcgcttga ttcaagaagt gcgcacagaa gcggctaaaa
    30181 aagtagaacg tgagcttgag ttggatatca tgggctgtga tattgatgct cgcatggtgg
    30241 aaattgctaa ggccaatgct caggcagctg gtgttgcagg agatattact tttaagcaga
    30301 tgcgcgtgca ggatttacgt tccgataaaa tcaatggagt aatcatttcc aatccgcctt
    30361 atggtgaacg tttgtcagat gatgcaggag tgaccaagct ctatgctgag atggggcaag
    30421 tatttgcacc gctgaaaact tggagcaaat ttatcctgac tagtgatgaa gcttttgaaa
    30481 gcaagtatgg tagccaagca gataagaagc gtaagttata caacggaacc ttgaaagtgg
    30541 atctatatca atattttggt cagcgtgtca aacggcaaga ggtaaaatag aaagggatac
    30601 tcatgagtaa aaaaagacga aatcgtcata aaaaagaagg tcaagaaccg caatttgatt
    30661 ttgatgaagc aaaagagcta acagttggtc aagctattcg taaaaatgaa gaagtggaag
    30721 caggagtctt gcctgaggat tccattttgg acaagtatgt taagcaacac agagatgaaa
    30781 ttgaggcgga taagtttgcg actcgtcaat acaaaaaaga ggagttcgtt gaaactcaga
    30841 gtctggatga tttaattcaa gagatgcgtg aggctgtaga gaagtcagaa gcttcttcgg
    30901 aggaagttcc atcttctgaa gacatcttac tccccttgcc tctggacgat gaggagcaag
    30961 gcttggatcc tctattgcta gatgatgaaa atccaacaga aatgactgaa gaagtggaag
    31021 aggagcaaaa cctttctcgt ctggatcaag aggactcaga aaagaaaagt aaaaaaggct
    31081 ttattttgac cgttttggcg cttgtatcag taattatttg tgtcagtgct tattatgtct
    31141 accgtcaagt ggctcgttcg accaaggaaa ttgaaacttc tcaatcaact acagccaatc
    31201 aatcggatgt ggatgatttt aatacacttt atgacgcctt ttacacaaat agcaataaaa
    31261 cggctttgaa aaatagccag tttgataaac tgagtcaact caaaaccttg cttgataaac
    31321 tagaaggtag tcgtgaacat acgcttgcca aatctaaata tgatagtcta gcaacgcaaa
    31381 tcaaggctat tcaagatgtc aatgctcaat ttgagaaacc agctattgtg gatggtgtgt
    31441 tggataccaa tgccaaagcc aaatcggatg ctaaatttac ggatattaaa actggaaata
    31501 cggagcttga taaagtgcta gataaggcta tcagtcttgg taagagccag caaacaagta
    31561 cttctagctc aagttcaagt caaactagca gctcaagctc tagtcaagca agttcaaata
    31621 cgactagtga gccaaaacca agtagttcaa atgatactag aagtagtcgc agtgaagtca
    31681 atatgggtct ctcgagtgca ggggttgctg ttcaaagaag tgccagtcgt gttgcctata
    31741 atcagtctgc tattgatgat agtaataact ctgcctggga ttttgcggat ggtgtcttgg
    31801 aacaaattct agcgacttca cgttcacgtg gctatatcac tggagaccaa tatatccttg
    31861 aacgtgtcaa tatcgttaac ggcaatggtt attacaacct ctacaagcca gatggaacct
    31921 atctctttac ccttaactgt aagacaggct actttgtcgg aaatggcgct ggtcatgcgg
    31981 atgacttgga ttactaagca gtcgttacaa aattctttct tttcaaaagt aaaaatgata
    32041 aaataaaaca aatgaaacaa gaggagtgtc aaatgacaaa agctaacttt ggtgtcgtag
    32101 gtatggccgt aatgggtcgt aaccttgccc ttaatattga atcacgtggt tacacagttg
    32161 ctatctacaa ccgtagtaaa gaaaaaacgg aagatgtgat tgcttgccat cctgaaaaga
    32221 actttgtacc aagctatgac gttgaaagtt ttgtaaactc aatcgaaaaa cctcgtcgta
    32281 tcatgctgat ggttcaagct ggacctggta cagatgctac tatccaagcc cttcttccac
    32341 accttgacaa gggtgatatc ttgattgacg gtggaaatac tttctacaaa gataccatcc
    32401 gtcgtaatga agaattggca aactcaggta tcaactttat cggtactgga gtttctggtg
    32461 gtgaaaaagg tgcccttgaa ggtccttcta tcatgcctgg tggacaaaaa gaggcctacg
    32521 aattggttgc ggatgttctt gaagaaatct cagctaaagc accagaagat ggcaaaccat
    32581 gtgtgactta tatcggtcct gatggagctg gtcactatgt gaaaatggtt cacaacggta
    32641 tcgagtacgg tgatatgcaa ttgatcgcag aaagctatga cttgatgcaa cacttgctag
    32701 gcctttctgc agaggatatg gctgaaatct ttactgagtg gaacaagggt gaattggata
    32761 gctacttgat cgaaatcaca gctgatatct tgagccgtaa agacgatgaa ggccaagatg
    32821 gaccaatcgt agactacatc cttgatgctg caggtaacaa gggaactggt aaatggacga
    32881 gccaatcatc tcttgacctt ggtgtaccat tgtcactgat tactgagtca gtgtttgcac
    32941 gctacatttc aacttacaaa gaagaacgtg tacatgctag caaggtgctt ccaaaaccag
    33001 ctgccttcaa ctttgaagga gacaaggctg aattgattga aaaaatccgt caagcccttt
    33061 acttctcaaa aatcatttca tacgcacaag gatttgctca attgcgtgta gcctctaaag
    33121 aaaacaactg gaacttgcca tttgcagata tcgcatctat ctggcgtgat ggctgtatca
    33181 tccgttctcg tttcttgcaa aagattacag atgcttacaa ccgtgatgca gaccttgcca
    33241 accttctttt ggatgaatac ttcttggatg ttactgctaa gtatcaacaa gcagtgcgtg
    33301 atatcgtagc tcttgcggtt caagctggtg tgccagtgcc aactttctca gcagctatta
    33361 cttactttga tagctaccgt tcagctgacc ttccagctaa cttgatccaa gcacaacgtg
    33421 actactttgg tgcccacact taccaacgta aagacaaaga aggaaccttc cactactctt
    33481 ggtatgacga aaaataagta ggtcagccat ggggaaacgg attttattac ttgagaaaga
    33541 acgaaatcta gctcattttt taagtttgga actccagaaa gagcagtatc gggttgatct
    33601 ggtagaggag gggcaaaaag ccctttccat ggctcttcag acagactatg atttgatgtt
    33661 attgaacgtt aatctgggag atatgatggc tcaggatttt gcagaaaaat tgagtcgaac
    33721 taaacctgcc tcagtcatca tgattttaga tcattgggaa gacttgcaag aagagctgga
    33781 agttgttcag cgttttgcag tttcatacat ctataagcca gtctttatcg aaaatctggt
    33841 agcgcgtatt tcggcgatct tccgaggtcg ggacttcatt gatcaacact gcagtctgat
    33901 gaaagttcca aggacctacc gcaatcttag gatagatgtt gaacatcaca cggtttatcg
    33961 tggtgaagag atgattgctc tgacccgccg tgagtatgac cttttggcga cacttatggg
    34021 aagtaagaaa gtattgactc gtgagcaatt gttggaaagt gtttggaagt atgaaagtgc
    34081 gaccgagaca aatatcgtag atgtctatat ccgctatcta cggagcaagc ttgatgttaa
    34141 aggccaaaaa agctacatta aaaccgtgcg tggtgttggt tacaccatgc aagaatagaa
    34201 aagcagttgc agttgtgtaa ctgctttttt gaggagtttt tatatattga catgcagtcg
    34261 ggtctttgct acaatcgttt taggaggata tatagatgaa acttttgaaa aaaatgatgc
    34321 aagttctact agcagtcttt ttctttggtt tgctagctac aaatacggta tttgcgaata
    34381 ccacaggtgg ccgatttgtt gataaggata atagaaaata ttatgtaaaa gatgatcata
    34441 aagcaatcta ttggcataaa atagacggta aaacttacta ttttggtgat attggagaga
    34501 tggttgtcgg ttggcaatac ttagaaattc ctggaacagg ttatcgtgat aatttattcg
    34561 ataaccaacc agttaatgaa attggccttc aggagaagtg gtactatttt ggacaagatg
    34621 gtgctttgct agaacaaaca gataaacaag tactagaggc aaaaacgtct gaaaatacag
    34681 gaaaagtata cggtgaacaa tatcctctat ctgctgaaaa gagaacttat tattttgata
    34741 ataattatgc tgtaaagaca ggctggattt atgaagacgg caattggtat tatttaaata
    34801 agctaggaaa ttttggcgat gattcttaca atccactacc aattggtgaa gttgctaagg
    34861 gttggactca agattttcat gttactattg acattgatag aagcaaacct gctccatggt
    34921 actacctaga cccagaaact ggcattatgc gaacaggatg gcaatatcta ggtaataact
    34981 ggtactacct ccgttcctca ggagcaatgg caacaggttg gaaatatgta cgaggcaaat
    35041 ggtattactt agataataaa aatggtgata tgaagactgg ttggtatcaa gatggttcaa
    35101 cttggtacta tttagatcct tctaatggag atatgaaaat aggttggaca aaagtaaatg
    35161 gaaaatggta ttatctcaat tcaaatggag caatggttac aggtagtcaa actatcgatg
    35221 gtaaagttta taacttcgct tcatctggtg agtggattta atgttggagg atatataaaa
    35281 tgaagctttt gaaaaaaact atgcaagctg gactaacagt aattttcttt ggtttgctag
    35341 ctacgaatac agtatttgca gataactctg aaggatggca gtttgtccaa gaaaacggta
    35401 gaacctatta caaaaagggg gacctcaaag aaacctactg gcgagtgatt gatggtaagt
    35461 actattattt tgattctcta tctggagaga tggttgtcgg ctggcaatat atcccgtttc
    35521 catctaaagg tagtacaatt ggtccttacc caaatggtat cagattagaa ggttttccaa
    35581 agtcagagtg gtactacttt gataaaaatg gagtgctaca agagtttgtt ggttggaaag
    35641 aattagagat taaaactcaa gacagtgttg gaagaaagta tggggaaaaa cgtgaaaatt
    35701 cagaagataa agaagagaag cgttattaca cgaactatta ctttaatcaa aatcattctt
    35761 tagagacagg ttggctttat gatcagtcta actggtatta tctagctaaa acagaaatta
    35821 atggagaaaa ctatattggt ggtgaaagac gtgcaggttg gataaatgat gcttcagctt
    35881 ggtactacct agatccagca actggtatta tgcaaacagg ttggcaatat ctaggtaata
    35941 agtggtacta cctccgttcc tcaggagcaa tggccactgg ctggtatcag gaaggtacca
    36001 cttggtatta tttagaccac ccaaatggcg atatgaaaac aggttggcaa aaccttggga
    36061 acaaatggta ctatctccgt tcatcaggag ctatggcaac tggttggtat caagatggtt
    36121 caacttggta ctacctaaat gcaggtaatg gagacatgaa gacaggttgg ttccaggtca
    36181 atggcaactg gtactatgct tatagctcag gtgctttggc agtgaatacg accgtagatg
    36241 gctattctgt caactataat ggcgaatggg ttcggtaatg acagggcgct tataattata
    36301 ttagaacact cgcttaatca aagaagtgac tttgttgtac ttgacctgac gattggggag
    36361 attaaaagca tgtgaaatct acacatgctt tttcgttagt tatgagtgtt gaacttgact
    36421 aaccattgac aaaagataag agcaatcaaa ataagataca aaaatatcaa tgctaactgc
    36481 accgaaacgc ccgatgctat ggatagggct aatagggaga gcagggacgg ggcgagaatg
    36541 tctactgtgc tcagagcaga gcccaccgtc cccatcttgt cactggggat atgggcaaag
    36601 acagctgctt gtaggcgagg gctgagaatg ccgataatcg tagaactgat gaaactggtc
    36661 acgaaaatta cgatataggc ttgacaaagg atactagtta ttagagccaa tagggaaatc
    36721 tcacagaaaa ccaagaggtg cgtgaaacgg atatttttaa atagattact gctggtcata
    36781 ttgccaagga tagctccaca agacatgatg atggagagca gggcgaagag ttgcccaatg
    36841 ctcagttgtg ctagtgtctg ttggatgaag cggagggcaa tcagccgagg ggacacatcc
    36901 aagatggcaa tcatactggt cgtcagaaac agtagtatga cggtatcttt taacctcagg
    36961 agcacagcca gcgactgttt aagatgttga aaatatttct taaaactcag tgctgtatgt
    37021 gacatttcaa tccttttttc aacctcatac aaggtatgtc gaataacata caggcccaaa
    37081 aaggcaatga caaaagtcag agtgttgata agggaaatag tttgaatact cataacattg
    37141 ataaggaatg cgccagctag attggcgaca atgcggacaa ccctcatcag agactgcctg
    37201 aaccccatgg catcatgcag gtcgtcctta attacactga tgtagagggc gttcatcatg
    37261 taggcagaca ggtagctcaa ggtgtcagag atgaggttga tgatgacaat cataatgact
    37321 gaaattaatg aaaagggcga caagacgaga aatagtgtca aaatagcgta gagcaggatt
    37381 ttgattttgg caatacagag tgcgtgtttg actctatttt cttgaaaatc tcctagaatc
    37441 ccgagaaaga gttgcgatag taggggaact atctctgaaa tggaaatcag taaaatcgct
    37501 agcggagcga aattgtactg agccacataa ttgataatgg cgaggtagaa aatactgtcg
    37561 ccgaatgatg aaaaacagct gtttagcagc aactgtctat atatgttgtt tgtccaaaat
    37621 aatttcatac ttttctcctt atgtctgcac atcgaatggg aatttattat ttaatagaca
    37681 ccaatatacc ccctgcttcc tattccgaag tcaaacaaat ctgctttacc actatctcct
    37741 tgaaacatca gtaaaccgtg atcatctcgt tcagaacatg ctaaaattac ctgttggatt
    37801 ttttcatcag taatttgttt tgattgtaga cagtggtttg tagtaagcta gttattccgt
    37861 ggcaatatcc tgtcatcatt tttagatgat ggttaaaaac aaattcttga tatttactaa
    37921 taatatcttt gtttccgtca caatcgtaca tacaaagata taagattatt ccggaaattc
    37981 cttcgcacca agaaagttgt aaatttccta aattttcagt attagaagta actttttcta
    38041 ataatttttt taattcagca tggaatgtat ggagatcatt atagaacata gaaggttcta
    38101 agactttaga ataggcaaat aaagcatatg caattccaca atatccatga gcaaagctat
    38161 agtctaaaat gctttctttg gctgtatctc tttgtaggaa ataatgataa atttccccat
    38221 atactattgt ggatgaaagt tttaagagta ttgtcattgg taaattgata gtattttatt
    38281 aatgataata ggacaccaga ttttcccaat gcaaagtcta tttcctctag agtatcataa
    38341 ttctctatta atttcttagc ggtgacgttt gctaattgta gatagtattg atttttactg
    38401 atagaaaata aatgtaggag taaccataga tagcctgcat caccaaatag cagtgagtat
    38461 tgatattcat atgtatgtgt cgttctaatt gtcactgcct tcttgatgat agagagtatt
    38521 ttagaatcca gttctggggg gtacaattta tttagaggaa acagtagtcc agctattccg
    38581 tgttggaatg ataaattact tacgaaattt ctaaattctg ttgaagaagt gatccttcct
    38641 tcagaattta cagttttatc tagtaaaaat tgtagaacac tattacattc tgttttgtaa
    38701 tcatatttat gttcctctat ttttttaatt ttgactaaat tatttgtttg acttaaattt
    38761 agtagacatt gtaaccaaaa tagttgctca tctgtgagct gaccttgagt atgtgatagt
    38821 tctagtaatt gaataggatc ctttaggtag attcaatcaa tctataacac aacaacataa
    38881 taacactttt aggataactt atcaaatatt atagtaaaat gaaataataa caggacaaat
    38941 cgatcaggac agtcaaatcg atttctaaaa atgttttaga agtagaggtg tactattcta
    39001 gtttcaatct actatagtat atatttttac atcttgaatt gtcaaattaa gtgcataagt
    39061 ttcccgtagc atacttcata aggcgttttc cacttaagaa attttcgtgg tcgattattt
    39121 agaacctgta ttcacaaaaa aatgttaagc tagagttatg atacgcaaag catatgatac
    39181 tgatttaaac gatcaagaat gggcgaagat tgaaccctat ttctccaaac accgcaccta
    39241 taaatggccg aaacgagtac ttgttaatga gactttgtac gtcactaaaa caggttgtca
    39301 atggcgcatg ctccctcacg actttcctct ttacttaatg gtatggagtt tctttcgtcg
    39361 ttctatgacg acaggttggt tccaggtcaa tggcagatgg tactacgctt atagcgctca
    39421 ggtgccttgg cagtgaatac gaccgtagat ggctattctg tcaactataa tggcgaatgg
    39481 gttcaataat gaaagaagcg attgtgaatg gaaacaatcg ctttttttgt gaaaatataa
    39541 taaaatagat aggaaagaat taatacttgt atgaaaaata agacgttttt tcgtctagta
    39601 aaaggaaaaa atgacaaaaa aagttggtgt cggtcaggca catagtaaga taattttaat
    39661 aggggaacat gcggtcgttt acggttatcc tgccatttcc ctgcctcttt tggaggtgga
    39721 ggtgacctgt aaggtagttc ctgcagagag tccttggcgc ctttatgagg aggatacctt
    39781 gtccatggcg gtttatgcct cactggagta tttggatatc acagaagcct gcattcgttg
    39841 tgagattgac tcggctatcc ctgagaaacg ggggatgggt tcgtcagcgg ctatcagcat
    39901 agcggccatt cgtgcggtat ttgactacta tcaggctgat ctgcctcatg atgtactaga
    39961 aatcttggtc aatcgagctg aaatgattgc ccatatgaat cctagtggtt tggatgctaa
    40021 gacctgtctc agtgaccaac ctattcgctt tatcaagaac gtaggattta cagaacttga
    40081 gatggattta tccgcctatt tggtgattgc cgatacgggt gtttatggtc atactcgtga
    40141 agccatccaa gtggttcaaa ataagggcaa ggatgcccta ccgtttttgc atgccttggg
    40201 agaattaacc cagcaagcag aagttgcgat ttcacaaaaa gatgctgaag gactgggaca
    40261 aatcctcagt caagcgcatt tacatttaaa agaaattgga gtcagtagcc ctgaggcaga
    40321 ctttttggtt gaaacgactc ttagccatgg tgctctgggt gccaagatga gcggtggtgg
    40381 gctaggaggt tgtatcatag ccttggtaac caatttgaca cacgcacaag aactagcaga
    40441 aagattagaa gagaaaggag ctgttcagac atggatagag agcctgtaac agtacgttcc
    40501 tacgcaaata ttgctattat caaatattgg ggaaagaaaa aagaaaaaga gatggtgcct
    40561 gctactagca gtatttctct aactttggaa aatatgtata cagagacgac cttgtcgcct
    40621 ttaccagcca atgtaacagc tgacgaattt tacatcaatg gtcagctaca aaatgaggtc
    40681 gagcatgcca agatgagtaa gattattgac cgttatcgtc cagctggtga gggctttgtc
    40741 cgtatcgata ctcaaaacaa tatgcctact gcagcgggcc tgtcctcaag ttctagtggt
    40801 ttgtccgccc tggtcaaggc ttgtaatgct tatttcaagc ttggattgga tagaagtcag
    40861 ttggcacagg aagccaaatt tgcctcaggc tcttcttctc ggagttttta tggaccacta
    40921 ggagcctggg ataaggatag tggagaaatt taccctgtag agacagactt gaaactagct
    40981 atgattatgt tggtgctaga ggacaagaaa aaaccaatct ctagccgtga cgggatgaaa
    41041 ctttgtgtgg aaacctcgac gactttcgac gactgggttc gtcagtctga gaaggactat
    41101 caggatatgc tgatttatct caaggaaaat gattttgcca agattggaga attaacggag
    41161 aaaaatgccc tggctatgca tgctacgaca aagactgcta gtccagcctt ttcttatctg
    41221 acggatgcct cttatgaggc tatggacttt gttcgccagc ttcgtgagaa aggagaggcc
    41281 tgctacttta ccatggatgc tggtcccaat gttaaggtct tctgtcagga gaaagacttg
    41341 gagcatttat cagaaatttt cggtcatcgt tatcgcttga ttgtgtcaaa aacaaaggat
    41401 ttgagtcaag atgattgctg ttaaaacttg cggaaaactc tattgggcag gtgaatatgc
    41461 tattttagag ccagggcagt tagctttgat aaaggatatt cccatctata tgagggctga
    41521 gattgctttt tctgacagct accgtatcta ttcagatatg tttgatttcg cagtggactt
    41581 aaggcctaat cctgactata gcttgattca agaaacgatt gctttgatgg gagacttcct
    41641 cgctgttcgt ggtcagaatt taagaccttt ttctctagaa atctgtggca aaatggaacg
    41701 agaagggaaa aagtttggtc taggttctag tggcagcgtc gttgtcttgg ttgtcaaggc
    41761 tttactggct ctgtatgatg tttctgttga tcaggagctc ttgttcaagc tgactagcgc
    41821 tgtcttgctt aagcgaggag acaatggttc catgggcgac cttgcctgta ttgtggcaga
    41881 ggatttggtt ctctaccagt catttgatcg ccagaaggtg gctgcttggt tagaagaaga
    41941 aaacttggcg acagttctgg agcgtgattg gggcttttca atttcacaag tgaaaccaac
    42001 tttagaatgt gatttcttag tgggatggac caaggaagtg gctgtatcga gtcacatggt
    42061 ccagcaaatc aagcaaaata tcaatcaaaa ttttttaagt tcctcaaaag aaacggtggt
    42121 ttctttggtc gaagccttgg aacaggggaa agccgaaaaa gttatcgagc aagtagaagt
    42181 agccagcaag cttttagaag gcttgagtac agatatttac acgcctttgc ttagacagtt
    42241 gaaagaagcc agtcaagatt tgcaggccgt tgccaagagt agtggtgctg gtggtggtga
    42301 ctgtggcatc gccctgagtt ttgatgcgga atcaaccaaa accttaaaaa atcgttgggc
    42361 cgatctgggg attgagctct tatatcaaga aaggatagga catgacgaca aatcgtaagg
    42421 acgagcatat cctctatgcc cttgagcaga aaagttccta taatagcttt gatgaggtgg
    42481 agctgattca ttcttccttg cctctttaca atctggatga aatcgatctt tcgacagagt
    42541 ttgctggtcg aaagtgggac tttccttttt atatcaatgc catgactggt ggaagtaata
    42601 agggaagaga aatcaatcaa aagctggctc aggtggcgga atcctgtggt attttatttg
    42661 taacgggttc ttatagcgca gccctcaaaa atccaacgga tgattctttt tctgtcaagt
    42721 ctagtcatcc caatctcctc cttggaacca atattggatt ggacaaacct gtcgagttag
    42781 gacttcagac tgtagaagag atgaatcctg ttctattgca agtgcatgtc aatgtcatgc
    42841 aggaattact catgcccgag ggagaaagga agtttagaag ctggcaatcg catctagcag
    42901 attatagcaa gcaaattccc gttcctattg tcctcaagga agtgggcttt ggaatggatg
    42961 ccaagacaat cgaaagagcc tatgaattcg gtgttcgtac agtggaccta tcgggtcgtg
    43021 gtggcaccag ctttgcctat atcgaaaacc gtcgtagtgg ccagcgtgat tacctcaatc
    43081 aatggggtca gtctaccatg caggcccttc tcaatgccca agaatggaaa gataaggtcg
    43141 aactcttggt tagtggaggg gttcggaatc cgctggatat gattaagtgc ttggtttttg
    43201 gtgctaaggc tgtgggattg tcacgaaccg ttctggaatt ggttgaaacc tacacagttg
    43261 aagaagtgat tggcattgtc caaggctgga aagcagatct acgcttgatt atgtgttccc
    43321 ttaactgtgc caccatagca gatctacaaa aagtagacta tcttctttat ggaaaattaa
    43381 aagaagcaaa ggatcagatg aaaaaggcgt aaccagcgcc tttttttcca tcctcagact
    43441 gaggtgactt ttttgaattg tgataaaata gaagggagag gatgaaccta tgagaaaatt
    43501 taaaatcttt ttatttatcg aagcctgtct tctgacagga gctctgattt tgatggtatc
    43561 agagcatttt tcgcgttttc tgctgatact attcctcttt ttgcttttga ttcgctacta
    43621 cactggtaaa gagggaaata atcttctttt agtagcggca accattctct tctttttcat
    43681 cgttatgctc aatccttttg tgattctagc tatttttgtt gcggttatct atagcctctt
    43741 tcttctttac ccgatgatga accaggaaaa agagcagacc aatttggttt ttgaagaggt
    43801 cgtgacggtt aagaaggaga aaaatcgttg gtttggaaat cttcatcatt tttcaagcta
    43861 ccagacttgc caattcgatg atatcaatct ctttcgcttc atgggtaagg acactattca
    43921 tctggagagg gtcatcttaa ccaatcatga caatgtcatt atcctcagaa agatggtagg
    43981 aacgaccaaa atcatcgtac ctgtagatgt ggaagtcagt ctcagcgtta actgtctcta
    44041 tggggatttg acttttttca accagcccaa gcgagccctc cgcaatgaac actatcatca
    44101 agaaacaaaa gactatctca agagtaacaa gagtgtcaag attttcttga ccactatgat
    44161 tggtgatgtg gaggtggtta gaggatgaaa aaacaagcct atgtcattat tgctctcacc
    44221 tccttcctat ttgtcttttt tttctcccat agcttgctgg aaatacttga ttttgactgg
    44281 tctatctttt tgcacgatgt cgaaaaaaca gaaaaatttg tctttttatt gttggttttc
    44341 agcatgtcca tgacctgtct cttagtcctg ttttggcgag ggatcgaaga gctttctcta
    44401 agaaaaatgc aggctaatct caagcgttta ttggcagggc aagaagtagt tcaggttgta
    44461 gatccagatt tggatgccag tttcaagtcc ttatcaggta aacttaacct tttgacagag
    44521 gctcttcaaa aagctgaaaa tcatagcctt gctcaggaag aggaaatcat cgagaaggaa
    44581 cggaagcgaa ttgctcggga tttgcacgat acagtcagtc aggagttgtt tgcggcccac
    44641 atgattttat cgggtatcag tcagcaggct ttgaaattgg atagagaaaa gatgcagacc
    44701 cagttgcaga gtgtcacagc tattttagaa acagcccaga aggatttgcg ggttttgctc
    44761 ttgcatttgc gaccagttga actggagcag aagagcttga tagaagggat tcaaattctc
    44821 ttaaaagagc ttgaggacaa gagtgatctt agggttagtc tcaagcagaa tatgacgaaa
    44881 ttgcctaaga aaatcgagga gcatattttc cgtatcctgc aagagttgat tagcaatacg
    44941 ctccgccatg cccaggcatc ttgcctagat gtctacctct atcagacaga tgttgaattg
    45001 caactgaagg tggtggacaa tgggattggt ttccagttag ggagcttaga cgacttgagt
    45061 tatggactgc gaaatatcaa ggagcgggtt gaagatatgg cagggacggt tcagttatta
    45121 acagctccca agcaagggct ggcagttgat atccgtattc ccctgttaga taaggaatga
    45181 taaaggagta aagatgaaaa ttttactagt agatgaccat gaaatggtcc gtttgggctt
    45241 gaaaagctac tttgacctcc aagacgatgt agaagttgtg ggtgaggcgt ccaacgggtc
    45301 tcaagggatt gacttggcct tggaactgcg tccagatgtc attgtcatgg atattgtcat
    45361 gcctgagatg aatgggattg acgcgacctt agcaatcctt aaagaatggc ctgaagccaa
    45421 gattttgatt gtgacctctt atttggacaa tgaaaaaatc atgccagtct tagatgctgg
    45481 tgccaaaggc tatatgctca agacttctag tgcagatgaa ttgcttcatg ccgtcagtaa
    45541 ggtagctgct ggcgagctgg ccattgagca agaggttagc aagaaggttg aataccaccg
    45601 caatcatatg gaacttcatg aagaattgac tgcgcgtgag cgagatgttc tccaactcat
    45661 cgccaagggc tacgaaaatc agcgcatcgc agatgacctc tttatctctc tcaagacggt
    45721 caagacccac gtgtccaata ttcttgccaa acttgaagtc agcgatcgta cccaggcggc
    45781 tgtctatgcc tttcagcacc atttggtggg gcaagaggag ttttagatga gtttagcaga
    45841 tttacttgag gagctagaag cagcaaaaga ctctaaaaaa gcaaggccca tggaagccta
    45901 tatgcgccat caattttctt ttctaggtat tgcggtgcct gaaagaaata aactctataa
    45961 aaatatttac tagaagcgaa aaaaacaaag attatcgatt gggattttgc agacacttgc
    46021 tgggaaaagg agcctagaaa ataccaatat gtggctgcta actatttgaa agcaatgcag
    46081 tcttatctaa aggaaaccga tttgcctaag ctagagcagt tagtcgtgac aaaatcctgg
    46141 tgggacacgg tagatatcct agatcgagta gtagggagtt tggtgtatga acaccctgaa
    46201 ctagaagaaa taatcttaat actcttcgaa aatctcttca aaccacgtca gctctatctg
    46261 caacctcaaa acagtgtttt gagcaatctg cggctagctt cctagtttgc tctttgattt
    46321 tcattgagta taaaatggag cctatcagat aatatctggc tgagacgtgt cgctattgac
    46381 caccagttgt taagaaaaga gaagaccaat actcaattaa tggaaaagat tctacttcat
    46441 aacttgaacc aaacagaatt ttttatcaat aaagccattg gctggattct gcgagactac
    46501 tccaaaacga atccaacttg ggtaacatgc tttattgatg aaaacaagga aagaatggct
    46561 gaacttagta tcaaagaagc aagcaagtac ctctagcgtg attgaaaagc gcagtcatta
    46621 attgaaaagt ttgcctgttt atgtgataat ggagggtatt taaagaaggt ggtagatttc
    46681 ttgattctat agtagattga gaaatttaga atagtacgct ataattacat taggaggata
    46741 cgataatgaa gaaaactgtt tataaaaaat tgggtatttc aattattgcg agtactttat
    46801 tggctagcca gttatcgaca gtatctgctt tgagtgttat ttctagtaca ggtgaagaat
    46861 atgaggtaag tgagacacta gaaaaaggtc cagagtctaa tgattcttca ttatctgaga
    46921 tttcaccaac gtatggttca tactaccaaa agcaatcaga agtattatcg gtaatgatga
    46981 tttgattaaa attgataata ctttacagta tccgtattcg acttcagcta tggttttatc
    47041 taagtattat ggagtagccg atggaatgaa tgtagaaggg aggggaagtg cgaattttat
    47101 taaagataat gtgttaatta cagcggctca caactactac agacatgact atgggaaaga
    47161 agcggatgat atttatgttc ttccggctgt tagtccaagt caagaacaat ttggaaagat
    47221 caaagtaaag gaagttcgtt atttgaagga atttagaaat ttaaattcta aggatgcaag
    47281 ggaatatgac ttggctttat taattctaga agagcccatt ggtgcaaaat tagggacttt
    47341 gggtcttcct actagtcaaa aaaatttgac aggaataact gtgactatca caggctatcc
    47401 atcatataat tttaaaattc atcaaatgta tacagataaa aaacaagttt taagtgatga
    47461 tggcatgttc ttggattatc aagttgatac tttagagggg tctagtggat ctacagttta
    47521 tgatgctagt caccgtgtag taggagtgca tactttagga gatggagcta atcaaattaa
    47581 cagtgcagtt aaattaaatg aacgaaattt gccatttatt tatttattcg gttcttaaag
    47641 gttactctct tgaaggatgg aagaaaataa atggtagttg gtactattat agacaacatg
    47701 ataaacaaac gggttggcag gagataaatg atacttggta ttatttagac agttccggta
    47761 agatgcttac agattggcaa aaagtaaatg gaaactggta ttatctcaat tcaaatggag
    47821 caatggttac aggtagccaa actatcgatg gtaaagttta taacttcgct tcatctggtg
    47881 agtggattta atgttggagg atatataaaa tgaagctttt gaaaaaaatg atgcaagtcg
    47941 cactagccac attttttttc ggtttgctag ggaccagtac agtatttgca gatgattctg
    48001 aaggatggca gtttgtccaa gaaaatggta gaacctacta caaaaagggg gatctaaaag
    48061 aaacctactg gagagtgata gatgggaagt actattattt tgatccttta tctggagaga
    48121 tggttgtcgg ctggcaatat atacctgctc cacacaaggg ggttacgatt ggtccttctc
    48181 caagaataga gattgctctt agaccagatt ggttttattt tggtcaagat ggtgtattac
    48241 aagaatttgt tggcaagcaa gttttagaag caaaaactgc tacgaatacc aacaaacatc
    48301 atggggaaga atatgatagc ccagcagaga aatgagtcta ttattttgaa gatcagcgta
    48361 gttatcatac tttaaaaact ggttggattt acgaagaggg tcattggtat tatttacaga
    48421 aggatggtgg ctttgattcg cgcatcaaca gattggcggt tggagagcta gcacgtggtt
    48481 gggttaagga ttaccctctt acgtatgatg aagagaagct aaaagcagct ccatggtact
    48541 atctagatcc aacaactggc attatgcaaa ctggttggca atttctaggt aatagatggt
    48601 actacctcca ttcgtcagga gctatggcaa ctggctggta taaggaaggc tcaacttggt
    48661 actatctaga tgctgaaaat ggtgatatga gaactggctg gcaatacctt ggtaacaagt
    48721 ggtactatct ccgttcatca ggagctatgg caactggttg gtatcaggaa ggttcgactt
    48781 ggtactatct aaatgcaagt aatggagata tgaaaacagg ctggttccaa gtcaatggta
    48841 actggtacta tgcctatgat tcaggtgctt tagctgttaa taccacagta ggtggttact
    48901 acttaaacta taatggtgaa tgggttaagt aatgaaggct aattgtaaac tgtgatggat
    48961 acttaacttt gtataatagg tggataaaag tcttcacaat caaaaaacgc atagtatcaa
    49021 ggtttttctg tactgccccc caaaagttag acaaataatt tatccaaagg atttagttct
    49081 gtactgcaca ggactaagtc cttttagttt taccttaatt ctcttgttgt tgtaataatc
    49141 aatatagtct ataatggctt gttccaattg attaagtgat ttaaatgttt tctcatagcc
    49201 ataaaacatt tccgatttca gaatcccaaa gaaggactcc accatagtgt ttctctgtga
    49261 atgcctgttc caacattgtt tgtacttgtt ctaagttggg tgaagttgaa agattatagg
    49321 cgataatttc gctgttaaag ccatctaaaa ctggtgataa gtaaagcttt tgagtacttg
    49381 ctggaatggc aaattctgtc acatctgtgt agcacttttc cattgtttta gagtcttcaa
    49441 attggccttg aatgagattc tctgccttct taccaacatc tcttttattt tcgtttctgt
    49501 cgcattttag cttgtaaatt gagtactttc atcaagactt gaactctttt atgatttatc
    49561 agataaccac gatttcttag ttctaagtaa atccgacgat aagtataatt tcccttgtgt
    49621 tcgataaaaa tggattgaat ttcagcttta agctcttggt ccttatctgg tttgtctagc
    49681 tgtttcaagt gatagtagta ggtcgaacga gctagtttaa tgacttttag aagaagagct
    49741 aacgaaaatt cagtcattaa ttcttgaaca atttctgtct ttcttctttc tctttttcct
    49801 ccttcaatcg gagttctctt aactttttta ggatagcatt ctccgctctc aggtactctc
    49861 cctcttgttt tctcaacaat agtatacccg tttttcctgt attgtgctag ccagttaaga
    49921 agtatcgtac gacttgggag gccgtattca agagaaactc tatctttagt ccagccttca
    49981 tgtcagactt tatgaatcat ttcttgtttt aaatcaggag gatagtaacg atttttccct
    50041 tttttgacga actctattcc gtaacgatca atcaatttaa tcatgtacct aagattagaa
    50101 ttgtttatcc caaatttatt tgaaagcttc tctaagctat atccttgttt tctaagttca
    50161 tagatctgaa ctttatcatc ataagttaat ttcataataa aaacacccca aaagttagat
    50221 tttttctgtc taacttttgg ggtgtagttc atgtacacct gatatgatgc gttttataat
    50281 ttttaagcct ttttgcccag cctcgtcaaa agtaatgttt tgacacaaaa tctgtgacaa
    50341 aactttagtt ttaaaggttt ttaactttgt atatactagt tttaagaaaa ggaggatgat
    50401 ctaatggaag aaaaagtatc attgaaggtc agggttcaaa aactagggac atcgctttca
    50461 aatatggtta tgcccaatat tggagcattt attgcttggg gagtattgac tgccctcttt
    50521 atcgctgatg gctatctgcc aaatgaacag ttagctactg ttgttggtcc tatgttaacg
    50581 tatttattgc caattctgat tggttacaca ggtggatata tgatccatgg ccaacgtggt
    50641 gccgttgtag gagctattgc tactgttggt gcaatcacag gttctagtgt tcctatgttt
    50701 atcggagcta tggtaatggg cccactggga ggatggacta tcaagaaatt tgatgagaag
    50761 ttcttggaaa aaattcgtcc cggatttgaa atgttagtta ataacttctc agctggtctc
    50821 gttggttttg cattattgct tttggctttc tacgcaatcg gtccagtcgt atcgactctt
    50881 actggagctg ttgggaatgg tgttgaggct attgtcaatg ctcgcctcct tcctatggct
    50941 aatattatca tcgaaccggc taaagtcctt ttcctcaata atgccctcaa tcatggcatt
    51001 tttactcctc tgggagtaga acaggtagct caagctggta agtcaattct cttcctattg
    51061 gaagctaatc ctggaccagg cctgggaatt ctattagctt atgctgtatt cggtaaaggt
    51121 tctgctaaat cttcttcttg gggggcaatg gttattcatt tcttcggagg gattcatgaa
    51181 atttactttc cttatgttat gatgaagcct actctatttt tagctgctat ggcaggaggt
    51241 atctctggaa cttttacttt tcaactctta gacgctggtc ttaaatctcc agcttcacca
    51301 ggttctatta ttgcgattat ggctacggcg ccaaaaggtg tttggcctca tctaaatgtt
    51361 cttttaggtg ttttagtggc agcagttgtt tctttccttg tagcagccct tattcttcat
    51421 gcagacaagt caactgagga ttcgctcgaa gctgctcagg cggctaccca agcagctaag
    51481 gctcagtcta aaggtcagtt agtatcaact tctgttgatg cagttgtttc gacagactca
    51541 gtggaaaaaa tcattttcgc ctgcgatgct ggtatgggaa gctctgctat gggagctagt
    51601 attcttcgag ataaggttaa aaaagcaggt ctagagattc cagtatctaa tcaggcaatc
    51661 tcaaatttgc ttgatacacc aaaaacatta attgttactc aggaagaact gacaccaaga
    51721 gctaaagaca agagtccaag tgctattcat gtttctgttg ataatttctt agcgtcccct
    51781 cgttatgatg aaattgtagc ttcattaaca ggagcttctc caatagcaga aattgaagga
    51841 gatataccaa cttcagcacc agtagatagt caggaaattg accttaacca tattgatgct
    51901 gtagtagttg cttatggtaa agcacaggga actgcaacta tgggctgtga aacgattcgg
    51961 gctatcttta gaaacaagaa tattcgtatt ccagtttcta ctgccaaaat ttcagaatta
    52021 ggtgaattta attctaaaaa cataatgatt gtaacaacta tttctttaca ggcagaagtg
    52081 cagcaagcag caccgaattc tcaatttctt attgtggata gtttagtaac aacaccagaa
    52141 tatgacaaaa tggctgctag aatgtacaaa tagaactaga ggtttctaaa ttacgaatgc
    52201 tattaaccaa acgagaagaa caattattga aggctttcct acatgtaggg aagctttcaa
    52261 tgcaagatat gactgaaatc ttacaggttt catctagaac aatttatcga actttatcag
    52321 atttgacaga tagcatggag caatatggaa tcgaaataac gaagcatggg aaatactata
    52381 ttttgactgg agagttggat gatttgccga cagaacttga agtgttagtt gagtatagtc
    52441 cccaagaaag acaagagttg attacctatc gccttctgac tgagagtggt tttgtcacca
    52501 atgaagcatt gcaagagtgc acgaaagtca gtaatgtaac tattattcag gatatttcag
    52561 atattgataa gcgtctttta gactttgatc tgaaaattga acgacaaaaa ggttatcgga
    52621 tttctggtga ttcagttggt aagagaagat ttttggctat tttactgaca aactgtatct
    52681 cggtagcaga tttttcaacc ggtaattttg ggagctttga tattttagaa gcagatagaa
    52741 ctaggctggc cagtcagatt gttaataagc aactgtcagg ttttccagat atggatgcta
    52801 ggatgaagat gttttttgcg atcttgttat ctcttatagg tcaggagcaa aacattgaaa
    52861 attcacctaa tactagtaag caggctttgg aaatttctca aaaaattttt caagcttact
    52921 ctaagcagac tgcacaattt tatagtattc aggaaattat ctattttgcg agcatcttgg
    52981 atgaattaat cattaaacgt caggacaatc cgctctttac ggagaaattt gatggtgaat
    53041 ttttctacaa tatttcaaat ctgattgata cggtttccat gtataccaag attgactttt
    53101 ttaaggacaa ggttttattc aattttcttt tccatcatat tcggctcagt ttaggcgtcc
    53161 ctatcctttt tcagggtgaa aatttgccag aatctgtcca gattttagtt gaaaggaata
    53221 aatttcttta tacagtcatc agtcttttag tgaatgatat ttttccgaaa tatcttcata
    53281 cagagtatga gtatggcatg attgccctac attttatctc tagcttaggc cgtagtccag
    53341 agatttatcc agtccgtgtt ttgcttttaa cggatgaacg tcgggtcact agagatttat
    53401 tagtcagtaa aattaagagt gttgctcctt ttgtagagtt gatagatatt cagtctctag
    53461 tagattacca cagtattgat ctcagtcagt atgattatat tttatctacc aagccgctga
    53521 ctaatcagga aatcgatgta atttctagtt ttccaaccgt caaagaattg cttgaattac
    53581 aggaacgact tcagtatgta caggcacatc gtacaattgt cgcgcgtgat gctatcgctc
    53641 cagagaaaag ttatgacttg caagattatt taatatctag tagtcagctt ttgagtcaat
    53701 ttgagttggt tcaattggag aataatcaat catttgagca cacggtagaa caaatcatcc
    53761 aatatcagaa gaatgtgagt gacagagatt acctaacaag aaaattgtta tctcacttcc
    53821 agaatagtcc tatggctatt cctaatactg gtctggtgct tttacatagt cagtctagca
    53881 aagtaacaac aaatagtttt actatgtttg aactcaaact acctatctcc gcattgtcaa
    53941 tgaaacgaga gaaagaagag gtcaaaaggt gtctgttaat gctaatgtct aaggaggcta
    54001 gcgaggaagt gagagattta atgacagcta tcagtcagtc gattattgaa aatcatcttt
    54061 atacagagat ttacaagacg ggaaatcaat ccattattta tcagatgcta aatactattt
    54121 ttaacgaaaa aattaagaaa ttggagaact aatatgaaac ttgaaaaaca tttgattaag
    54181 cttgataaac aattttctaa caaggaggaa gctatccgtt attgtgggca agttctttat
    54241 gagggtggat atgttaatga agactatatt gaagccatga ttgagcgtga taaagagcta
    54301 tctgtttaca tgggtaactt tatcgccata ccgcatggaa cagatgcagc gaaaaaggat
    54361 gtcctcaagt ctggtattac agtcgttcaa gtcccaagag gggttgattt tgggaatgaa
    54421 tctaacactc aagtggcaac ggttcttttt ggtattgctg gtattggtaa tgaacactta
    54481 gaaattattc agaaaatttc tatcttctgt gcagatgtgg ataatgttct taaactagca
    54541 gatgctcagt caaaagagga agtattgcgc ttatttgatg ctgttgaata attgaatttc
    54601 gtcatttgtc atctagtata tatgtccctc aaataggaaa aggagaaatt gaatgaaaca
    54661 ttctgttcat tttggtgccg gtaatatcgg tcgtggtttt ataggtgaaa ttctatttaa
    54721 aaatggtttc catattgatt ttgtggatgt caataatcag ataattcatg ctctaaatga
    54781 aaagggcaag tatgaaattg aaattgcaca gaaaggacag tctcgtatag aagtaactaa
    54841 tgtggctggc attaatagca aagaacatcc tgagcaagtc attgaagcga ttcaaaagac
    54901 ggatattatt actactgcaa tcggacctaa tatactccct tttatcgctg aacttctagc
    54961 caaaggaatc gaagctcgcc gagttgcagg gaatacacag gtattggatg ttatggcctg
    55021 tgaaaatatg attggcgggt ctcaatttct ttatcaagaa gtcaagaaat atttaagtcc
    55081 ggaaggtttg acatttgctg ataactacat aggttttcca aatgctgcag tagacaggat
    55141 tgttccagca caaagtcacg aagattccct ttttgttgtg gtcgagccct ttaatgaatg
    55201 ggtcgtggaa accaagcgtc ttaaaaatcc agatttacgt ctaaaagatg tgcattatga
    55261 agaagattta gaacccttta ttgagcgaaa acttttttca gtcaattctg gacatgcaac
    55321 ttcagcttac attggtgcgc attatggtgc caagacaatt ttggaagctc ttcaaaatcc
    55381 taatattaaa tctcggattg aatctgtatt agctgaaatt cggagtctct tgattgccaa
    55441 atggaacttt gataaaaaag aattggagaa ttatcacaaa gtcattatag aacggtttga
    55501 aaaccctttc atagtggacg aggttagtcg cgtagctcgt actccaatcc gaaaattagg
    55561 ctataatgaa cgattcatcc ggccgatacg tgaattgaaa gaactcagtt tgtcatataa
    55621 aaacctactt aaaacagttg gctatgcctt tgactatcgc gatgtaaatg atgaagaaag
    55681 tattcgatta ggtgaattgt tggctaaaca atcagtcaaa gatgttgtta tacaagttac
    55741 aggtttagac gaccaagaat tgattgagca aattgtagag tatatttaat ctttttcgaa
    55801 aatctcttca aatcaggtta gcatcgcttt gtcttaggca tatgttgttc tatctacaac
    55861 ctcaaagcag tgctttgagc tgactccgtc agtcttgtct gcaacctcaa aacactgttt
    55921 gagttatctg cggtaatctt tctagcttgt ctttgatttt tgttgttatt tataaggtaa
    55981 aagaagctgg acaaaaagtc ttcaaaatcg gaaaaaggca gcctatcggg tgttcaaaaa
    56041 tcttgatagg atgtccttta ttatggaaag ccttattgga ttttctcctc agattgagtt
    56101 tttgatcagc tttatgagat aggttttgct agagatgtag cccatcatgt tatttttatg
    56161 gacggtggga aaattgttga aaaaaataat gcccatcaat tctttagtcg tccaagagaa
    56221 gaacgaacca agcaattttg gaacgaattc tttcgaatgc gatctatata gtaaaatgaa
    56281 acaaaaacag gacaaatcga tcaggacagt caaatcgatt tctaaaaatg ttttagaagt
    56341 agcagtgtac tattctagtt tcaatctact atataactga aaaattagat aaattagttt
    56401 tggaaaatga ctaaccaaaa gatatccaaa gtagtctaaa attgtctata ctttatgagt
    56461 gttttagtta ggaaaaaggc ttgttgtcta taattgtctg cattagtcta gattttattt
    56521 atagaaaatg ttataataga ctgtatttaa aaaattttaa ggagaaatga cagaatgtct
    56581 gtatcatttg aaaacaaaga aacaaaccgt ggtgtcttga ctttcactat ctctcaagac
    56641 caaatcaaac cagaattgga ccgtgtcttc aagtcagtga agaaatctct taatgttcca
    56701 ggtttccgta aaggtcacct tccacgccct atcttcgacc aaaaatttgg tgaagaagct
    56761 ctttatcaag atgcaatgaa cgcacttttg ccaaacgctt atgaagcagc tgtaaaagaa
    56821 gctggtcttg aagtggttgc ccaaccaaaa attgacgtaa cttcaatgga aaaaggtcaa
    56881 gactgggtta tcactgctga agtcgttaca aaacctgaag taaaattggg tgactacaaa
    56941 aaccttgaag tatcagttga tgtagaaaaa gaagtaactg acgctgatgt cgaagatcgt
    57001 atcgaacgcg aacgcaacaa cctggctgaa ttggttatca aggaagctgc tgctgaaaac
    57061 ggcgacactg ttgtgatcga cttcgttggt tctatcgacg gtgttgaatt tgacggtgga
    57121 aaaggtgaaa acttctcact tggacttggt tcaggtcaat tcatccctgg tttcgaagac
    57181 caattggtag gtcactcagc tggcgaaacc gttgatgtta tcgtaacatt cccagaagac
    57241 taccaagcag aagaccttgc aggtaaagaa gctaaattcg tgacaactat ccacgaagta
    57301 aaagctaaag aagttccggc tcttgacgat gaacttgcaa aagacattga tgaagaagtt
    57361 gaaacacttg ctgacttgaa agaaaaatac cgcaaagaat tggctactgc taaagaagaa
    57421 gcttacaaag atgcagttga aggtgcagca attgatacag ctgtagaaaa cgctgaaatc
    57481 gtagaacttc cagaagaaat gatccatgaa gaagttcacc gttcagtaaa tgaattcctt
    57541 gggaacttgc aacgtcaagg gatcaaccct gacatgtact tccaaatcac tggaactact
    57601 caagaagacc ttcacaacca ataccaagca gaagctgagt cacgtactaa gactaacctt
    57661 gttatcgaag cagttgccaa agctgaagga tttgatgctt cagaagaaga aatccaaaaa
    57721 gaagttgagc aattggcagc agactacaac atggaagttg cacaagttca aaacttgctt
    57781 tcagctgaca tgttgaaaca tgatatcact atcaaaaaag ctgttgaatt gatcacaagc
    57841 acagcaacag taaaataatc ttaataaaca gaaaacccac ctgaattggt gggttttctg
    57901 atgtactatt ttccaaaaat ctctttgagg tctgtgtctg taatcccaat catggctgga
    57961 atgctgtccc agttttcttc ggttaggatg taggattgtt cagaggcact tgatgtgact
    58021 gtttcagaga cagcttgttg cttttcttca acattctcca gtagatcact gaagcgttca
    58081 atcagatagg tttttcgggc agttccgatg tgttgggtag catagtcgaa ggcttgtaat
    58141 tcgcctagta agatgagttt gcttttggca cgtgtaatgg ctgtgtagat gagatttcgc
    58201 tccagcatac gtcggctagc actagtaatc ggtaggatga caactgggaa ctcacttccc
    58261 tgagacttat gaatactcat ggcataggcc aagcgaatct tgtaccattc gttacggggg
    58321 taagagactt cattaccatc aaaatcaatg acaatctcgt cttgtttcga ttcggtgtat
    58381 ttgccaggaa tcaggtctgt gatagctcct aaatccccat taaagacatt gatttcagca
    58441 tcgttaacca aatgaatgac cttgtctctc ttacgatagt ggcactgagg agcttcaaaa
    58501 ctgagttgat ctttttgtgg gggattgagc aggtcttgca tgagctgatt gatagcatca
    58561 atccctgccg tccctcggta cataggagcc agaacttgga tatcacgggc aggaatacca
    58621 tttctgaggg cgacacctaa gattttttca atggtggcag gaatatggcc actagcaatt
    58681 tcaaagtagg aacggtcagc ttttttttgg gtgaaatcag ctggcaagat gccctgtcga
    58741 atctgactag ctagggtgac gatggttgat tctttgcttt gtcgataaat tttttccaag
    58801 cgagtctgag gaatcaaagg aatatgaagc agatccgcta gaacctgtcc aggactgaca
    58861 gaaggtagct gatcactgtc acctacgatg aggatcttac tgttagaaga gatattggag
    58921 aagagttgat tggccagcca agtatctacc atagagaatt catccacgat gataaagtca
    58981 gcatctaggt aatcttccag atgactggta tcatcgtcac ctgtcattcc caagtggcgg
    59041 tgtatggtcg cgctaggcaa acctgtcaat tcattcatgc ggcgagcggc tcgtccagtt
    59101 ggagcagcaa gaagaatggg cagattgctt ttcttcctga ggtcaagtcc ctctaacagg
    59161 gcgtaaacag cgatgatccc attgataaca gttgtcttac cagtaccagg tccacctgtc
    59221 aggataaaga ccttattctg gatagcatca cagatagcct gtttttgaat gttatcatac
    59281 tcaattccca gctcttcctc gatagtagtg atatgttttt gaatggtttc taaatcatga
    59341 ctcttctgtt ttcctttttc aaggatacga accaagtgac tgcggatgcc ttcctcagcg
    59401 aaaaagaggc tgttgtcaaa gatcttggta tcaatctgct gaaccttgtc ttcttcgatc
    59461 aggtaggaga gctcttgggc aacttggctg gggtctagtt ctacgggacg ggaagactca
    59521 aggagagtaa gggtttgttc cagcaaatcc cgtgcttcaa cataggtgtc ccctgtttcc
    59581 atacaggcct gaaaaagact gtgaactaga ccggcgcgga agcgttcagg agcctgactt
    59641 tcgatgccta gttcctcagc tagttggtca gcaatggtaa agcccaaacc cttgatatcc
    59701 tcaaccagct ggtagggata attttcaacc acatcaaggg tttcttcctt gtaaaagtct
    59761 tgaatctgaa aggctagttt gttgggaatg ccgtagttgg ctagtttggc caaaaccatc
    59821 tccgttccgt agttgagacg gagagtggag acgaaagcct cgcgattttt ggcagagagt
    59881 cctgcgatgc cttctaactt ttctgggtgt tgcaaaattt cgtcaatggt attttcgcca
    59941 taggtatcca cgattttctg agctgtcttg agaccaatcc ccttgaaatg gctacttgaa
    60001 aagtacttga ccaagccctt actagttggt tttgcgcgat cataacgact gatttgcagt
    60061 tgttctccat acttggagtg ctggacaatt tgcccccaaa aagtatagtc ttcgccctca
    60121 attacatcag ccatggttcc tgtgacaatg atttcaaagt catcaaaatc ctctgcgtcc
    60181 gtatcgtcga tttctaggag gaggatgcga taaaaattgc tgggattttc aaaaataatc
    60241 cgttcaatgg ttcctgaaaa ataaacttcc ataaaattcc tttgcatgaa taggtgagag
    60301 ttgggattgt ttttatttta tactcttcga aaatatcttc aaaccacgtc agcttccatc
    60361 tgcaacctca aaacagtatt ttgagctgac ttcgtcagtt ctatccacaa cctcaaaaca
    60421 ctgttttaag cagcctacgg ctagcttcct agtttgttct ttgattttca ttgagtattt
    60481 gtcaataaac aatcacttct cacgatagaa gaagaggctg agattggtga ttctcggcct
    60541 cttaggtctc ttaaaatgtt ccgatacggg tgattggcca gaagcggaat ttagcttccc
    60601 ctgtgatatc ttttgctttg aaggtaccta cgtggcggct gtcgctcgaa accaagcggt
    60661 catctccgag gagaaggtat tctccttctg gaacagtaaa gctaaagttg gtgttgtagt
    60721 tgacatcaac tgtgaaggct tgggcttttt gagcgatact tctaaagaaa gttcctttat
    60781 ttccttcaaa gcccttgcct gagtaagtgc tttggagttt gtcatccttg aagcgtttga
    60841 tatagtctgc tagataaggc tcgtccgttt ctttgtcatt gatgtaaagt ttatcatttt
    60901 cgtaacgaat ggtgtcgcca ggcattccaa tcacgcgctt gacgatgtcc ttattgccat
    60961 cttcctcatg ggcaaccacg atatcaaaac ggtcaatagg aaggtgtttt acaacgaaga
    61021 gaatttcgcc atccgctagg gtcggatcca tggaatgtcc ttctacgcga acattgctcc
    61081 aaaaaaagat acgacttaaa gctagtaatg acagaattag gaggaacaat ccccactctt
    61141 ttaagaaatt tttaaatgaa ttcataactt acctttctaa gcgttttttc gctttttcag
    61201 tgtttttaaa gtgcaatttg gcgcagaagc tgagtccctg cataccatag gcttgcaaaa
    61261 tctggctagc caccttgtca gaagccgttc cagctccact tgggagctga taacccagtt
    61321 ctcgtcccaa attttcaaga ttttccagaa agagatcacg cgcaatgaca gaagaaactg
    61381 cgacagccaa gtatttgccc tcagcctttt cttctaagct gataggattg ctgaaacgat
    61441 tggcttcttg tgccaagtac ttgtcataat ttttagcact ggtaaaggca tcaatcacaa
    61501 ttttctcagg ctgaacacct ttttgaagga ggagatagat agcctgatta tggagggcaa
    61561 ccttaaccga aacagcattg tagcggtctc cgatgacctc gttgtacttg ctgggtgaga
    61621 gaaggagtgc ctggtgctgg attttttcct tgagaatagg agcaatctga cgaatctttt
    61681 ggtcggtcag agtcttagaa tctcccacac caagttttcg taagaaggca tgctggtcag
    61741 gtgtgacaaa ggaagccaca actgcaagcc caccaaagta ggaaccattc cctacctcat
    61801 ctgtcccaat caaaggaaga ttttgccccc tgttttcctc tacaagttga tagccaaaga
    61861 aactggcgta tttttcagcc ccttcaccct gaagcaagat ttttccagaa gtatagatag
    61921 aaaccgttgc ttgaggtagt ttcaaaaagt agcggatata gggattcttg ctaggagcca
    61981 gactggtttg atagtgttca agaaaagcct gaatatcctt ttcgcttggt gtgagtgtga
    62041 tacttgccat agtttctatt gtaccacaaa agcagtaaaa tttgtaaaaa ctgacaaaat
    62101 tagcgaattt tggtataata tcgtgaggtg aattttatgg caaatctaaa tcgattcaaa
    62161 tttacattcg ggaaaaaatc gttaaccttg acaagcgaac atgataacct ttttatggag
    62221 gaaatcgcta aggttgcgac agaaaaatac caagcaatta aagaacaaat gcctagcgca
    62281 gatgatgaaa caatcgctct tttgttggca gtcaactgtt tatcaactca gctcagccgt
    62341 gagattgaat ttgacgataa ggagcaagag ctagaagaac tccgtcacaa gcttgtgact
    62401 tgtaagcaag aacagagcaa gattgaggat tccttatgat ttcattcctt cttctattgg
    62461 tcttggtttg gggattttat atcggctatc ggagaggcct gctcttacag gtttattacc
    62521 tgatttcagc catggcatcg gcttttatgg ctggccagtt ttataagggg cttggagagc
    62581 aattccattt attgctccct tatgcaaatt cgcaggaaga tcaggggact ttctttttcc
    62641 catcggatca actctttcag ctggataagg tcttttatgc aggtatcggc tacttgcttg
    62701 tatttgggat tgtctatagc attggtcgtt tgcttggtct tctcttacac ttgattccta
    62761 gcaaaaaact gggtggtaag ttgttccaag tttcagcagg tatcttgtcc atgttggtga
    62821 ccttatttgt cttgcaaatg gccttgacaa tcttggcgac catccccatg gcagttatac
    62881 aaaatcctct tgaaaagagt atcgtcgcaa aacacatcat ccagagcata ccgataacaa
    62941 ccagttggct caaacaaatc tgggtgacaa atttaatcgg ataaaaaggg caggagtttt
    63001 cctagccctt tgtttacaga tttgactcga atctatcaga atgtaaaaag ctaccacacc
    63061 tagacattca aagacaagga aataaagatg aataagaaaa tattagaaac attagagttc
    63121 gataaggtca aggccttgtt tgagcctcat ttgttgaccg agcagggctt ggagcaattg
    63181 agacagctgg ctccgactgc caaagcagat aaaatcaaac aggcttttgc tgagatgaaa
    63241 gaaatgcagg ctcttttcgt cgagcaaccg cattttacta ttctctcaac taaggaaatt
    63301 gcaggagtct gcaagaggtt ggagatggga gcggatctca atatcgagga gttcctactc
    63361 ttgaaacgcg tgcttcttgc cagccgagaa cttcaaagtt tttatgccaa tctggaaaat
    63421 gtcagcttgg aagaattagc cctttggttt gagaaattac atgattttcc gcaattacaa
    63481 ggaaatcttc aggcctttaa tgatgcgggt ttcattgaaa attttgccag tgaagaattg
    63541 gcgcgaatcc gtcgaaaaat acatgatagc gagagtcagg tacgcgatgt tttacaagac
    63601 ttactcaagc aaaaagcgca gatgttgacg gaaggaattg ttgctagcag aaatggccgt
    63661 caggttttac cagtcaaaaa cacctaccgc aataagattg caggtgtcgt tcatgatatt
    63721 tctgctagtg gaaacaccgt ctatatcgaa ccacgtgagg tagtcaaact gagcgaagaa
    63781 attgctagtc tgcgagcaga tgagcgctat gaaatgcttc gcattctcca agaaatttct
    63841 gagcgtgtcc gccctcatgc gactgagatt gctaatgacg cttggattat cggtcatctg
    63901 gacttgattc gtgccaaggt tcgatttatc caagaaagac aagcagtcgt gcctcagctg
    63961 tcagaaaatc aagagattca actgctccat gtctgccatc ctttggtcaa aaatgccgtc
    64021 gcaaatgatg tctattttgg tcaagattta acagctattg tcattacagg tcccaataca
    64081 ggtgggaaga ccatcatgct caaaactctg ggcttgacac aggtcatggc ccagtcagga
    64141 ttaccgattt tagcagacaa gggaagtcgt gttggtattt ttgaagaaat ttttgctgat
    64201 attggagatg agcagtctat tgagcagagc ttgtctacct tctctagcca tatgaccaat
    64261 atcgtggata ttcttggcaa ggtcaaccaa cattcactct tacttttgga tgagttgggg
    64321 gctggtactg atccccaaga gggagcagcc cttgccatgg ctattctgga ggaccttcgc
    64381 ctgcgtcaaa tcaagaccat ggcgacgacc cactatccag aactcaaggc ctacggtatt
    64441 gagacagcct ttgtgcaaaa tgccagtatg gagtttgata ctgcaactct tcgcccgacc
    64501 tatcgcttta tgcagggtgt tcctggccga agtaatgcct ttgaaattgc caaacgtcta
    64561 ggcctatctg aagttatcgt aggagatgcc agtcagcaga tcgatcagga caatgacgtc
    64621 aatcgtatca ttgagcaatt agaagagcag acgctggaaa gccgcaaacg tttggacaat
    64681 atccgtgagg tggagcaaga aaatctcaag atgaaccgtg cgctaaaaaa actctacaac
    64741 gagcttaatc gtgaaaagga aaccgagctt aacaaggcgc gtgaacaggc tgctgagatt
    64801 gtggatatgg ccctaagtga aagtgaccag attctcaaaa atctccacag taaatcccaa
    64861 ctcaagcccc acgaaatcat tgaagccaag gccaagttga aaaaattggc tcctgaaaaa
    64921 gtggacttgt ctaaaaataa ggtccttcaa aaggccaaga aaaaacgagc tccaaaggtg
    64981 ggagatgata tcgtggttct cagttatggt cagcgtggta ccttgaccag tcaactcaag
    65041 gacggtcgct gggaagccca agttggcttg attaagatga ccttggaaga gaaagagttt
    65101 gatcttgttc aagcccagca agaaaaacca gtcaagaaga aacaggtcaa tgttgtgaaa
    65161 cgaacttctg ggcgaggacc tcaagctaga ctggatcttc gaggcaagcg ctatgaagaa
    65221 gccatgaatg agctagatac cttcatcgac caagctttgc ttaacaatat ggctcaagtt
    65281 gatatcatcc atggtatcgg aacaggagtc atccgtgaag gagttaccaa atacttgcaa
    65341 agaaacaaac atgtcaagag tttcggctat gccccacaaa atgctggagg cagtggtgcg
    65401 actattgtca cttttaaagg atagcagtat tctggacttt ataaagtaaa aactgttgaa
    65461 ctaattttta ctaataaaca cattgacaaa agccaacatt ttttgtaaaa ttagaatcaa
    65521 ttaataccaa caccgaatga agtttaatag aagtggggaa tcgtttgatt ttccatgact
    65581 gtaaatggac ggaactctgg agagaccgta aaggcaccga aggggcaagg caggcaactg
    65641 ctcaaactct caggtaaaag gacagagcta ggatagaccg ctttttagca tttatctaag
    65701 cattccagag tacatgtatc ttgcatgtgc tctttctttt ggggttgaaa cgataggaga
    65761 aggaaatgtt agaattgctt aaatcaatcg atgcttttgc ttggggaccg cccctcttga
    65821 ttttattggt cggaacaggg atttacctaa ccatgcggct aggactcttg caggttttgc
    65881 gtctgcccaa ggcctttcag cttattttta tccaggataa gggacatggt gatgtatcca
    65941 gttttgcagc tctgtgtaca gccttggcat caactgttgg aacaggaaat atcataggag
    66001 ttgcgacggc tatcaaggtt ggtggaccag gagctctatt ttggatgtgg atggcggctt
    66061 tctttggaat ggctaccaag tatgcggaag gactcttggc catcaaatac cgcaccaagg
    66121 acgaccatgg tgcagtagcg ggaggtccca tgcactatat ccttctaggg atgggagaaa
    66181 agtggcgacc acttgctgtt ttgtttgcag tagcaggagt attggttgct ctcttgggaa
    66241 tcggaacctt cacccaagtc aactcgatta cagaatctat ccaaaataca acgacgattt
    66301 cgccagccat cacagctctc gtcttgtctg tctttgtagc gattgcagtc tttggtggac
    66361 tcaagtctat ttctaaggtt tcaactactg ttgttccttt tatggccctc atttatatct
    66421 taggaactct tacagttatt ttctttaata tcggaaaaat ccctggcaca atcgctttag
    66481 tctttacctc agcttttagt ccccttgctg cggtaggtgg atttgctggt gctagcgttc
    66541 ggatggctat tcaaaatggt gtggcgcgtg gtgtgttctc aaacgaatct ggtctgggtt
    66601 ctgctcctat tgcagctgca gctgccaaga caaatgaacc agtagagcaa ggtttgattt
    66661 ccatgacagg aacctttatt gataccctca tcatttgtac tctaactggt ttgaccatct
    66721 tggtaactgg agtttggagt ggtgacttga atggggttgc cttgactcag tcagctttct
    66781 caacagtctt ttcacacttt gggcctgccc tcttgaccat cttccttgta ctttttgcct
    66841 ttacaacgat tctaggttgg aactattacg gagaacgctg ttttgagttc ctctttgggg
    66901 ttcgctttat ctggctctac cgtgtggttt ttgtgctcat ggtcttgtta ggaggattta
    66961 tcgagttgga tatggtctgg attatcgcag atatcgttaa cgccttgatg gctctgccaa
    67021 acttgattgc cctcttggtc ttgtcgccag tcgttattgc tgaaactaaa aagtattttg
    67081 acaaataatg gaatcacaca gctcgtgtga tttttttgcc tataaataat ttttatcagc
    67141 ctgatatata atatatatta tgcaagtgat tggaagtgtg ataaactaat ataacaacga
    67201 aaatcttatg agagaataaa gctttatctt agataaactt aggaatcagg attttataag
    67261 attttccaat aatattagtg tcaataataa gaaaagaggt atcttatgac aacatttact
    67321 atccatacag tagagtcagc accagcagaa gtgaaagaaa ttcttgaaac agtagaaaaa
    67381 gacaacaatg gctatattcc caacctaatc ggtctcttgg ccaatgctcc gactgtttta
    67441 gaagcctatc aaattgtctc atctatccac cgtcgcaaca gcctgacacc cgttgagcgt
    67501 gaagtggtgc aaatcacggc agccgtgacc aatggttgtg ccttctgtgt cgcaggtcac
    67561 acagcctttt ccatcaaaca aatccagatg aatgatgact tgattcaagc tcttcgcaat
    67621 cgtactccaa ttgaaacaga tcctaaattg gataccctag ctaagtttac cttggcagtt
    67681 atcaatacca agggtcgtgt aggagatgaa gccttgtctg agtttttaga agctggctac
    67741 actcaacaaa atgccttgga tgtggttttt ggtgtcagcc tagcaatcct ctgtaactat
    67801 gccaacaact tagctaatac accaattaat ccagaattgc aaccttatgc ctaattcata
    67861 tctgagtaaa atgaagtctg aaataagcgg acttcgtttt tttaaatcct catttaagcg
    67921 cttttatgtt atactgaaac taacatgatt attggaggtt aggatgaaaa aactcccctt
    67981 agtattttca ggttgtttgc taggtttggc aggagctgga aatcttattt tagatacgtt
    68041 gccggttcta tcccatcttt ttagtctgac tggtttggtt ttatggattt actttctaat
    68101 tctgcatctc tttaattgga aagaaaccaa gcaagaattg accaagcccc ctcttttgtc
    68161 aggaatggca acctttccta tggctgggat gattttatcg acctatgtct ttcgcgtatt
    68221 ctcttatctt cctttggtag cacaagggat ttggtggttt tcatttctct tggatttgac
    68281 cttgattgct ggttttacca tcaagtttgc ttgtccaggg cggagggttc atgccactcc
    68341 aagttggacg gttctctatg tggggatagc agtggctgcc ttgacctatc ctctggtagg
    68401 tattatcgaa attgcctatg cgaccttgag ttttggtttt ctcttgacct tctatctcta
    68461 tccccttatt tatagcgatt taaagaaaca tccactccca ctagccttgc ttggacaaga
    68521 aggaatctac tgtgctcctt tctctctact cttggcttct ctagttcgag taggaggaac
    68581 cagcctgccg acttgggtct tgattgtcat gattttggct tctcaatcct tctttttctt
    68641 tgttttaact cgtctgccca acattttaaa acaaggtttt caaccagcct tctcagccct
    68701 caccttccca accattatca cagcgacctc gctcaagatg gctcagggaa ttttgaaact
    68761 tccatttctg gattacctgg tattggctga aaccattata tgcctaacta ttttattctt
    68821 tgtactaggt gcttatctga tttggttacg aaaaaaggtc tagctagaaa tagctaaacc
    68881 ttatttttta tggtttgatg acttcagctc cacccatgta tggacgaagt gcttctggga
    68941 tggtcacaga agcatcttca ttttggtaat tttcaagaat tgcagccact gtacgtccaa
    69001 ctgcaagtcc agaaccgttc aaggtatgaa ggagtttcac cttgccatct gcttcatcac
    69061 ggtaacggat ttgggcacga cgggcttgga aatcttctgt gtttgaacag cttgagattt
    69121 cacggtaatt gttttgtgct ggaatccaca cttccaagtc gtaagtcttc gcagctgaga
    69181 agcccatatc tccagtagag agagcaacga cacggtatgg aaggttgagt ttttgaagaa
    69241 tgttttcagc gttggctgtc attttttcca attcttcgta agattcttct ggtttggcaa
    69301 atttgaccat ttcaaccttg tggaattggt gcaaacggat caagccacgc gtatcacgac
    69361 cggcagaacc agcctcagaa cggaatgacg gactcatggc agtgaagtag attggaagat
    69421 ctttgccgtc taagatttca tcacggtagt agtttgtcag aggaacttca gcagttggaa
    69481 tcaagacaaa gttggtatcg ctgagttcaa aagtatcttc cttaaatttt ggatactgac
    69541 cagtaccaaa catagaatca tggttgacta tgtaaggtgt gatgacttca gtatagcctt
    69601 cttttccatg ttcatccaac ataaagttgt agatagcacg ttccaaacga gcaccgaggc
    69661 ctttatagaa gaggaagcga gcgcctgtta ccttaccacc gcgttcccag tcaaggatac
    69721 caaggtcttc accgagatcc cagtgagctt taggttcgaa gtcaaactcg cgtggagtac
    69781 cccaacggcg aacttccaca ttgtcgtctt cgtcagcccc aacaggaacg ctgtcagctg
    69841 ggatatttgg aagagtcgtt gtaaattctg tcaatttagc atcgatttct gccaattcag
    69901 catccaaggc tttaacctca gcagatagat tttgcatggc agcaatcttg tcatctgtat
    69961 tttccttgtt gcgcttagct tgggcaatct cagcagaaac tgtgttacgt tctgctttga
    70021 gagtttcaac cttaatcaag atgttacgac gtttagcatc gatttctttc atttcattca
    70081 agacagcagc atctacacca cgtgtagcta atttttctgc gacagcttca aaatctgtac
    70141 gaatacgttt gatatctaac ataagaactc ctttatgaaa aaagcacgcc tgacaaaacg
    70201 ttggagtggc agggccacgg ttccatccaa cttcacaggt gtgcacttga ttgtgtatgt
    70261 aattgttact aacggtagaa tttcacctat ccctcctatc tgctcgcagt acccgcagac
    70321 tttctgaaag aagaagataa cctacttatc cgttgctatg attatactaa agtttctact
    70381 tttttgcaaa tagattttta aatttttggc taattgtctg aatcagggtc ggaagtttga
    70441 cgaccttgtc attgcctagt ttttcgcgtg caattttgag aatggcacct gagtcttttg
    70501 aagcaaagag gaattttcct ttgtctgtaa agacttcgaa gtggcggctg attttgcgtc
    70561 cagtgacatt ggctccaatc tgattgatat ggctccaagg aatctggata aattgttcga
    70621 cattgacatc tgggtaaaat tccaaagcct gatctccgac aaggaatttc ccaactttcc
    70681 cagcgataga gaggtaggaa gtgcctgtcg tactgaggag tactgttttg ttaagtgatt
    70741 gggccatgct tagtcttcct tactttctcc aaaaaaggca ttgtagaggg ctttaattgc
    70801 tgctttctct tggtctttat tgacaacaaa cataatagaa acttcactag aaccttgaga
    70861 catcatctgg atgttgattt tgttttcaga tagagcgcgt gtcgcagtag cagtcactcc
    70921 gatgtggctc ttcatttttt caccaacaat cataatgata gaaaggtcgt gttcgatttc
    70981 tgcatgatct actttagcct tttgaaccaa ctgacgcagg atttcttctt ccttgatggg
    71041 agttagttgg cgagaacgga gaatgataga aagatcgtcg atacctgttg gcatatgttc
    71101 ccaaccgatg ttaagttctt ccaggatttg cagaaccttg cggccaaatc caacctcacg
    71161 gttcatgagg tatttcgaca tgttaatgct gacaaagcct gagtcaccag caattcccac
    71221 aactggaaat tcatcattac tgtgttttag aacgatacga gtacctggat ggtcagggtt
    71281 gttggtattc ttgataacca gaggaatttt tccacggtag gcaggaagaa gagcctcgtc
    71341 atgaaggact gagaagcctg cataggccaa ctcgcgcatt tcacggtagg tcaactcagg
    71401 aatcgagtgt ggttggtgga taataccagg gtgggctgca aagataccat caacgtccgt
    71461 aaagttttca tagaggtcag ctttgacacc agcagcaatg atagaacctg taatatcaga
    71521 acctccacgt gagaaagtac agatttgatt ttccttagtg acaccaaaga aaccaggaat
    71581 gacaaggact tcattggtgt ttgtcaattc ttcaatcttg tcataacttg atggaatgat
    71641 gcgagcgtga ccaggttcac ttgtgaccac aatcccagct tctctagggt gcatatagcg
    71701 tgcatcgata ccattttggt taaagtaggc agcaatcaat ttggcattgt tattttcacc
    71761 ggctgctagg aaagtatcgt agagaaattc attttcttca ataggaagag tggccaaggc
    71821 gtgaatactt ttagaaattt tttctagcac agctggtttt agtcccaatt cactaaccat
    71881 agcagcatag cggtcgataa tccagctttg gctcttgcta atatcgttac ccgcaacata
    71941 gtcgcggtag tatttaatca gggcatccgt aaccttagta tcttcagcat tgcgtttacc
    72001 aggcgcagaa acgactacaa aacgacgctc tgaatcgctt ttgacgatgt ttaaaacttt
    72061 ttctaattga ctagcagagg caagagaact acctccaaat ttaacaactt tcataagaac
    72121 tcctaaagta agtattttat acgattatag cagaaagaaa ggcttttttc aatgaagaaa
    72181 ataagatgct ttctagccta aagcgagcct ttcttatcgg ctgagacact ttcagtcgat
    72241 tttttcttgc tttctgctcc taaaaaagat atactttgac agtgaaataa tttaacactc
    72301 aatgaaatca aagagcaaac taggaagcta gccgcagact gctcaaagca ctgctttgag
    72361 gttgtaaata agactgacga agtcagctca aaatactgtt ttgaggttgc agatggaagc
    72421 tgacgcggtt tgaagagatt ttcgaagagt ataaactgct tataaaataa aaaggagccc
    72481 tgatggaaca cattatttat cagcttgaag aggacttggc aatccttacc ttgaaccgtc
    72541 ctgaggtcgc aaatggtttt catattccca tgtgtgagga gattttagaa gctctgactt
    72601 tggcagaaga aaatccagct gtgcatttta tcttaatcaa tgccaatggg aaggtcttct
    72661 cagttggggg agatttggta gagatgaagc gggcagtgga tgaggatgat attccatcat
    72721 tgacaaaaat cgcagagttg gtcaatacga tttcttataa aatcaagcaa atagccaaac
    72781 ctgttttaat ggaagttgac ggtgctgttg caggtgccgc agcgaatatg gctgttgcgg
    72841 cagatttctg tttggcgacg gataaggcta agtttatcca agcctttgtt ggtgttggtt
    72901 tggctccaga tgcaggggga attcatctct tgagccgcag tatcggtgtg acgcgtgctg
    72961 ctcaattagc tatgacagga gaggctttaa cggcagaaaa agccctagaa tgggggttgg
    73021 tttaccgcgt ttctgaagct gaaaaacttg aaaagacgag agaacagctt cttaaaaaat
    73081 taagacgtgc ttcaagtaat tcctatgctg ccattaagaa gttggtttgg gagagccaat
    73141 ttaaagactg gcagggttac gctactttag aactgaacct acagaaatcc ttagctcaaa
    73201 cagaggattt caaagaagga gtgcgagctc attcggagag aagaagacct aaatttacag
    73261 gaaaataaaa aatacttgca tcattctttg aattttgata taattctttc tgtcaaatgt
    73321 tttgattgta aaagtttttt gaagaaggag ggatatgaaa aatggactac caacgaatca
    73381 atgaatattt aacatctata tttaacaatg tccttgtaat tgaggaagtg aacttgagag
    73441 gtagtcgttt taaggatatc tccatcaaag aaatgcatac gattgatgtc atcggtaagg
    73501 ctccagatgt gactccaagt caagtgtcaa aagagttgat ggtaactctt ggaactgtta
    73561 cgacaagttt gaacaattta gagcgtaagg gctacattga gcgagttcgg tcagaacagg
    73621 atcgtcgtgt ggtgcatctg catttgacaa agaagggtcg cttgattcat agactgcata
    73681 aacgcttcca caaggccatg gtagaaaaaa ttattgatgg catgagcgag gaagaaattg
    73741 ctgtcatggg taaaggcttg actaatcttt accaattttt ggaggatttg aaataatggc
    73801 ttttgcaaaa ataagtcagg ttgctcatta tgtgccagag caagtggtta caaatcacga
    73861 cttggctcag attatggata ccaatgatga gtggatttca agtcgaacgg gaatacgaca
    73921 aaggcatatt tcaagaacag aatctaccag tgatttggct acagaggttg ctaagaaact
    73981 gatggcaaaa gctggaataa caggagaaga actggatttt atcatcctag ctaccattac
    74041 tccagattcg atgatgccct ctacagctgc tcgtgttcaa gctaatattg gtgctaataa
    74101 agcctttgct tttgacttaa ccgcggcttg cagtggattt gtatttgctc tttcaactgc
    74161 tgaaaagttt atcgcttctg gtcgctttca aaaaggcttg gtgattggta gtgaaaccct
    74221 ctctaaggca gtcgattggt cggatcgatc aacagctgtg ttgtttggag atggtgctgg
    74281 tggtgtcttg ttagaagcta gcgagcaaga gcatttctta gctgagagtc tcaatagcga
    74341 tggaagtcgc agcgagtgtt taacttatgg gcattcaggt ttgcattctc cattttcaga
    74401 tcaagaaagt gcagattcat ttttgaagat ggatggacgc acagtctttg attttgccat
    74461 tcgagatgta gccaagtcta tcaagcagac tattgatgaa tctcctatag aggtgacaga
    74521 cttggattat ctgctacttc atcaagccaa tgaccgtatt ttggataaga tggctagaaa
    74581 aattggtgtt gaccgagcca aacttccagc caatatgatg gaatatggca ataccagtgc
    74641 agccagtatc ccgattttac tttcagagtg tgtagaacaa ggtctcatcc ctttagatgg
    74701 tagccagact gttcttctat caggcttcgg tggaggcttg acctggggca cgctcatttt
    74761 tacaatttag gtaatcatgt ggtgaacaca ttgttataaa aaaactattt attttaaagg
    74821 agtcctatca tggcagtatt tgaaaaagta caagaaatta tcgttgaaga acttggaaaa
    74881 gacgcatcag aagtaacact tgaatcaact tttgatgatt tggacgcaga ttcattggac
    74941 ttgttccaag taatctcaga aatcgaagat gcttttgata tccaaatcga agcagaaaat
    75001 gacttgaaaa cagttggtga cttggttgct tacgttgaag agcaagcaaa ataagcagaa
    75061 tattagtgga aggagtaggg agaaacccac tccctcttgt ttaggtaata gtttgactgt
    75121 caaattatgg tgaaatcgaa ctattacgta agcaaggaag gttggaggca ctatgaaaac
    75181 gcgtattaca gaattattga agattgacta tcctattttc caaggaggga tggcctgggt
    75241 tgctgatggt gatttggcag gggctgtttc caaggctgga ggattaggaa ttatcggtgg
    75301 gggaaatgcc ccgaaagaag ttgtcaaggc caatattgat aaaatcaaat cattgactga
    75361 taaacccttt ggggtcaaca tcatgctctt atctcccttt gtggaagata tcgtggatct
    75421 cgttattgaa gaaggtgtta aagttgtcac aacaggagca ggaaatccaa gcaagtatat
    75481 ggaacgtttc catgaagctg ggataatcgt tattcctgtc gttcctagtg tcgctttagc
    75541 taaacgcatg gaaaaaatcg gtgcagacgc tgttattgca gaaggaatgg aagctggggg
    75601 gcatatcggt aaattaacaa ccatgacctt ggtgcgacag gtagccacag ctgtatctat
    75661 tcctgttatt gctgcaggag gaattgcgga tggtgaaggt gctgcggctg gctttatgct
    75721 aggtgcagag gctgtacagg tggggacacg gtttgtagtt gcaaaagagt cgaatgccca
    75781 tccaaactac aaggagaaaa ttttaaaagc aagggatatt gacactacga tttcagctca
    75841 gcactttggt catgctgttc gtgctattaa aaatcagttg actagagatt ttgaactggc
    75901 tgaaaaagat gcctttaagc aggaagatcc tgatttagaa atctttgaac aaatgggagc
    75961 cggtgcccta gccaaagcag ttgttcacgg tgatgtggag ggtggctctg tcatggcagg
    76021 tcaaatcgca gggcttgttt ctaaagaaga aacagctgaa gaaatcctaa aagatttgta
    76081 ttacggagcc gctaagaaaa ttcaagaaga agcctctcgc tggacaggag ttgtaagaaa
    76141 tgactaaaac agccttttta tttgctggtc aaggtgccca gtatctaggg atgggacggg
    76201 atttctatga tcagtatccg attgttaaag aaacgattga tcgagcgagt caggtgctag
    76261 gttatgattt gcgttatctc atcgatacgg aagaagacaa actcaatcag acccgctata
    76321 cgcaaccagc cattctagcg acttcggttg ctatctaccg tttattgcaa gaaaagggct
    76381 atcagcctga tatggttgct ggtttgtctc ttggagaata ctctgccttg gtggcaagcg
    76441 gcgccttgga ttttgaagat gcggttgtct tggtagctaa gcgtggagcc tatatggaag
    76501 aagcggctcc tgctgactct ggcaagatgg tagcagttct caatacgcca gtagaggtca
    76561 ttgaagaagc ctgtcaaaaa gcttctgaac ttggagtggt tactccagcc aactataaca
    76621 cacctgcaca aatcgtcatt gctggagaag tggttgcagt tgatcgagcg gttgaacttt
    76681 tgcaagaagc aggtgccaaa cgcttgattc ctcttaaggt gtcaggtccc tttcacaccg
    76741 ctctccttga gccagctagc cagaaactag ctgaaactct agctcaggta agtttttcag
    76801 attttacttg tcccctagtc ggcaatacag aagctgctgt gatgcaaaaa gaggacattg
    76861 ctcagctctt gacgcgtcag gtcaaggaac ccgttcgttt ctatgaaagt attggggtca
    76921 tgcaagaagc aggcataagc aactttatcg agattggacc ggggaaagtc ttgtcaggtt
    76981 ttgttaaaaa aattgatcaa actgctcact tagctcatgt ggaagatcaa gcgagtttag
    77041 tagcactttt agaaaaatag actaaaataa gtagaagttt tgaaaggaaa aaaatgaaac
    77101 tagaacataa aaatatcttt attacaggtt cgagtcgtgg aattggtctt gccatcgccc
    77161 acaagtttgc tcaagcagga gccaacattg tcttaaacag tcgtggggca atctcagaag
    77221 aattgctcgc tgagttttca aactatggta tcaaggtggt tcccatttca ggagatgtat
    77281 cagattttgc agacgctaag cgtatgattg atcaagctat tgcagaactg ggttcagtag
    77341 atgttttggt caacaatgca gggattaccc aagatactct tatgctcaag atgacagaag
    77401 cagattttga aaaagtgctc aaggtcaatc tgactggtgc ctttaatatg acacaatcag
    77461 tcttgaaacc gatgatgaaa gccagagaag gtgctatcat taatatgtct agtgttgttg
    77521 gtttgatggg gaatattggt caagctaact atgctgcttc taaggctggc ttgattggct
    77581 ttaccaagtc tgtggcacgc gaggtcgcta gtcggaatat acgagtcaat gtgattgctc
    77641 caggaatgat tgagtctgat atgacagcta tcttatcaga taagattaag gaagctacac
    77701 tagctcagat tccgatgaaa gaatttgggc aggcagagca ggttgcagat ttgacagtat
    77761 ttttagcagg ccaagattat ctaactggtc aagtgattgc cattgatggt ggcttaagta
    77821 tgtagcgaaa gctagaggtg aaaagaatga aactaaatcg agtagtggta acaggttatg
    77881 gagtaacatc tccaatcgga aatacaccag aagaattttg gaatagttta gcaactggga
    77941 aaatcggcat tggtggcatt acaaaatttg atcatagtga ctttgatgtg cataatgcgg
    78001 cagaaatcca agattttccg ttcgataaat actttgtaaa aaaagatacc aaccgttttg
    78061 ataactattc tttatatgcc ttgtatgcag cccaagaggc tgtaaatcat gccaatcttg
    78121 atgtagaggc tcttaatagg gatcgttttg gtgttatcgt tgcatctggt attggtggaa
    78181 tcaaggaaat tgaagatcag gtacttcgcc ttcatgaaaa aggacccaaa cgtgtcaaac
    78241 caatgactct tccaaaagct ttaccaaata tggcttctgg gaatgtagcc atgcgttttg
    78301 gtgcaaacgg tgtttgtaaa tctatcaata ctgcctgctc ttcatcaaat gatgcgattg
    78361 gggatgcctt ccgctccatt aagtttggtt tccaagatgt gatgttggtg ggaggaacag
    78421 aagcttctat cacacctttt gccatcgctg gtttccaagc cttaacagct ctctctacta
    78481 cagaggatcc aactcgtgct tcgatcccat ttgataagga tcgcaatggg tttgttatgg
    78541 gtgaaggttc agggatgttg gttttagaaa gtcttgaaca cgctgaaaaa cgtggagcta
    78601 ctatcctggc tgaagtggtt ggttacggaa atacttgtga tgcctaccac atgacttctc
    78661 cacatccaga aggtcaggga gctatcaagg ccatcaaact agccttggaa gaagctgaga
    78721 tttctccaga gcaagtagcc tatgtcaatg ctcacggaac gtcaactcct gccaatgaaa
    78781 aaggagaaag tggagctatc gtagctgttc ttggtaagga agtacctgta tcatcaacca
    78841 agtcttttac aggacatttg ctgggggctg cgggtgcagt agaagctatc gtcaccatcg
    78901 aagctatgcg tcataacttt gtaccaatga cagctgggac aagtgaagta tcagattata
    78961 tcgaagctaa tgtcgtttat ggacaaggct tggagaaaga aattccatac gctatttcaa
    79021 atacttttgg ttttggaggc cacaatgcag ttcttgcttt caaacgttgg gagaatagat
    79081 aagtatgaat ttaaacgata ttaaagactt gatgactcaa tttgaccagt caagtttgag
    79141 agaattttct tataaaaatg ggacggatga gttgcagttt agtaagaatg aagcgagacc
    79201 tgtgcctgaa gttgcaactc aagtcgctcc agcacccgtt ctagcaacac cgagtccagt
    79261 agctcctaca tctgctccag cagagactgt agcagaagaa gttccagctc cagctgaagc
    79321 aagtgtggct agtgagggaa atcttgtaga gagtccactt gttggagtgg tttacttggc
    79381 tgctggacca gataaacctg ccttcgttac agttggtgat agtgtcaaaa aaggtcaaac
    79441 attggtaatt atcgaagcca tgaaagtcat gaatgaaatc ccagctccta aggatggtgt
    79501 ggtaacggaa attctcgtct ctaacgaaga aatggttgag tttggtaaag gattggtacg
    79561 tatcaaatga tcgatattca aggaatcaaa gaagctcttc cccaccgtta tcctatgctt
    79621 ctagtggacc gtgtcttgga agtgagcgag gataccattg ttgctatcaa aaatgtgacc
    79681 attaacgaac ctttctttaa cggccacttt cctcaatacc cagttatgcc aggtgttctg
    79741 attatggaag ccttggcgca aactgctggt gtgttggagt tatcaaaacc tgaaaataaa
    79801 ggaaaactgg tcttttacgc tggtatggac aaggttaagt tcaagaagca agttgtacca
    79861 ggcgaccaat tggttatgac agcgactttt gtaaaacgtc gtggcaccat agctgtggtt
    79921 gaagcaaagg ctgaagtgga tggcaagctt gcagccagtg gtacccttac ttttgcaatt
    79981 gggaactaag gaggttctcc atgtttcgaa aaattttaat tgccaatcgt ggtgaaattg
    80041 cggttcgtat tatccgtgcg gcacgtgaat tggggattgc gacggtagcg gtttattcaa
    80101 ctgctgataa ggaagctctt catacgcttt tggcagatga agcagtttgt attggtcctg
    80161 gcaaggcaac agagtcttat ctcaatatta atgcagttct atcagctgca gtcttgactg
    80221 aggcagaagc tattcaccct ggttttggat ttctcagtga aaattccaaa tttgcgacca
    80281 tgtgtgaaga agtaggtatc aagtttatcg gtccatctgg tcatgttatg gatatgatgg
    80341 gggataagat caatgcgcgt gctcagatga ttaaagcagg tgtgcctgtt ataccaggtt
    80401 cagatggaga agtgcataac tctgaagaag ctttaattgt tgctgaaaaa attggctatc
    80461 ctgttatgct caaggcttca gcaggtggag gtggtaaagg gattcgtaag gttgaaaaac
    80521 cagatgacct cgtttctgcc tttgaaactg cctctagtga ggccaaggcc aattatggca
    80581 atggtgccat gtacatagag cgggttatct atccagctcg gcacattgag gttcaaatcc
    80641 taggtgatga gcatggacat gtgattcact tgggtgaacg ggattgttct cttcaaagga
    80701 ataaccaaaa ggttttggaa gaaagtccct cgattgcaat cggaaaaacg ctgcgtcatg
    80761 aaataggtgc tgctgctgtt cgagcggcag agtttgttgg ctatgagaat gcaggaacca
    80821 ttgaatttct tcttgatgaa gcaagtagaa atttctattt catggagatg aatactcgtg
    80881 ttcaggtaga acatccagta acagagtttg tttcaggtgt tgatatcgtt aaggaacaga
    80941 tttgcattgc ggcaggtcag cctttgtctg ttaagcaaga agatattgtc ctacgcggtc
    81001 atgccatcga gtgtcgtatc aatgcagaaa acccagcctt taactttgct ccaagtccag
    81061 gtaagattac taatctctat ctgccaagtg gtggagttgg cttgcgcgtg gattcagcag
    81121 tttatccagg ttataccatt ccgccttatt atgatagtat gattgccaaa atcatagtac
    81181 acggcgaaaa tcgttttgac gccttgatga aaatgcaacg tgccctctat gaattagaga
    81241 ttgaaggagt gcagaccaat gcagatttcc agcttgacct catttcagat cgcaatgtca
    81301 ttgctgggga ttatgatact tccttcttga tggaaacctt cttacctaaa tatcaagaaa
    81361 aagaataaaa tgactttaag agtttaaacc gaaaagggga atcaatggct ctatttagta
    81421 aaaaagataa gtatattcga atcaatccca atcgttcggt tagggaaaaa cctcaagcta
    81481 agccagaggt tccagatgaa ttattttccc agtgtccagg ctgtaagcat accatctatc
    81541 agaaggatct gggaagtgaa cgtatctgtc cgcactgtag ctataccttt cgtatttctg
    81601 cccaagaacg cttggctttg acgattgata tgggaacctt caaagaattg tttacaggga
    81661 ttgaaagcaa ggatcccttg catttccctg gttaccaaaa gaaactggca tctatgcgtg
    81721 aaaaaacagg tctgcatgaa gccgttgtga caggaactgc tcttattaaa ggtcagactg
    81781 tggctctcgg gattatggat tctaacttta tcatggcttc tatgggtacg gttgtaggtg
    81841 aaaaaatcac tcgtttgttt gagtatgcga ctgtcgaaaa attgccagtt gtcctattca
    81901 cagcctctgg tggagcccgt atgcaggaag gaatcatgag tctcatgcag atggctaaga
    81961 tctctgcggc ggttaaacgt cattcaaatg ctggtctctt ttacctgacc attttgacag
    82021 atccaacgac tggtggtgtg acagcttctt tcgctatgga aggcgatatc attctggctg
    82081 aaccacagag cttggttggt tttgctgggc gtcgtgtgat tgaaaatacg gttcgtgaaa
    82141 gcttgcctga ggatttccaa aaggcagaat tcctattaga acatggcttt gtggatgcta
    82201 ttgtcaaaag aagagactta ccagatacga ttgctagcct agtcagattg catggaggga
    82261 gtcctagatg aatattgcaa aaatagtcag agaagcgcgt gagcagagtc gcttgacaac
    82321 cttggacttt gcgacaggca tttttgatga atttatccaa ttacatggtg accgttcttt
    82381 tcgtgatgat ggtgcagttg ttggtggtat tggttggctt ggagatcaag ctgtaacagt
    82441 ggttggtatc caaaaaggca agagtttgca agacaacctc aaacggaatt ttggccaacc
    82501 acatccagaa ggctaccgaa aggcactgcg gttgatgaaa catgctgaga aatttggccg
    82561 tccagttgtg acctttatca atacagcagg tgcttatcct ggtgtcggag cggaagaacg
    82621 tggtcaaggg gaagctatcg ctcgcaatct catggaaatg agtgacctga aagttccgat
    82681 tattgctatc attatcggtg aaggtggttc aggtggggct ctggctctag cagtcgcgga
    82741 ccgtgtctgg atgctggaaa attctattta tgccattcta agtccagaag gctttgcttc
    82801 cattctatgg aaggatggca gtcgtgccat ggaagcggca gaattgatga aaatcacttc
    82861 gcatgagctg ctagaaatgg acgtggtgga taaggtgatt tctgaagtag gactttctag
    82921 taaagaactg attaagagtg tcaaaaaaga actccaaacg gagctggcta gactttcaca
    82981 aaaaccgcta gaagagttgc tggaagaacg ctatcaacga tttagaaaat actaagaatg
    83041 actaaatgag actagaaagg tctcattttt tatggaaaaa tgcaattcag gttgaaaaat
    83101 tgacagttga ggagatagac tataaaatag acaaaaaaga tgtatactgt agatgtaagc
    83161 ttacaaaaat ttatagtgta ttgaatctat aacagtacac cttggctgct aaaatatttc
    83221 tataaattaa tttgactttc ctgatagagt tgttcacatc ttatttcaaa tcactatatt
    83281 ttaagaaggt acttgcaatg acagctacag aaacatttac agttatttca actgaggact
    83341 tggagcaaac ttcaggtggt cttgctgttt gggaagatgg atatagtaga tggttatatt
    83401 atagagaatt tgctccttat atgaggcaag gggcacttaa ttcttatata gatgcttgga
    83461 agtacggctt ccgaacaggg taatctttca aaaaagtacg atgagaaaca caaagaaact
    83521 aagacaattt ggaatatttt tgcttattat tttactatct acctatttac cacaaacaat
    83581 tggattgtat gtgacgatca ttttagggtt aggagctgat gtctattctc ttattttaac
    83641 agtgggatta gtgggaagtt tccttttcta ttaatttgga ggttaaaaaa gaaaaagagc
    83701 tgggactggt catttgtctt ctccttattt gcaacttatg tggtttatca gattctcggt
    83761 aatttttggg cacgctatgc tcatttgatt aatcatagga atattcatga tgagtatttt
    83821 accgtggttc tttcaaatgg acaaccaacc tttttatcga ctatcttatc ttttgtcctg
    83881 cctgtaatca tcggtcctgt ttttgaggag actcttgata gagggtattt tatgaacacc
    83941 ttctttccct agtccaagta ttatttggat gttatcctat ctgctatcat ctttgggatt
    84001 agccatttga tactatctca cagagatcca attagtttat tatattatag tctgattggt
    84061 ttcttttttg cccttgttta tcgttctacc gacaatctga gattaacgat tctctgccat
    84121 agttttttta actttctcaa tcatgcaaaa cctatctgga tttttgttta caattatatc
    84181 tattatcatt tttttagata gtgggaggca aagaaaaaag tgtttgatat ctatcaaaca
    84241 ctttttcttt ttattgttct tctgttacaa actggctgag cagtccattg ataaaacggg
    84301 cagatttttg atcggagaag tcctttgcaa gctcgatagc ttcattaaca gcaaccagct
    84361 gaggagtgtc aaatgaagtg atttcaaaga ctcccaagcg aaggaggttt ctctccacga
    84421 gcgttaagcg ttcaatggtc caacctgctt ttaaatgctg agtgatttgc ttatctagtt
    84481 cttccttttt agcttgaaca ccagaaacga ggtctatcaa aaaggctgga agttgtacat
    84541 ccgtatcttc acgatcatga gtataggcga aacgacaagc agtttcgaca tccgtaccga
    84601 actcaaggct catgagagct tgaaaagcgc atttacggag ttggcgtcta gattctaata
    84661 gtggactagt cattgaggaa gtcctcgtca aatagatctt tcaattctgg ttttggtgtt
    84721 ttatctggga cgatacctgc aacgtgaata ttgatagcag cgagttctac atcagccata
    84781 ttacggacgg catctttgac agctttctgg atagcaaccg ctaccttagg aacttttact
    84841 ccgtactcaa ggtagagata gatatctgct gtgagttctt cgtccacgtt tttaagataa
    84901 atgccacggc cgagtgaaag ttttgaaagg gtatcagaca ctgatctgtt tgaaaaagag
    84961 tgaacaccct ctacctttgc agtagcgata gcaatgattt tttcaagtac acgtggggcg
    85021 ataacgattt cgccaagttg ttcttcaatt cccatagaat gacctctttc tagagattag
    85081 gcacgagaaa cgtaagttcc ttctgcagtg ttgataacga gtttttgtcc tgcttcgatg
    85141 aagtctggaa cgtttacgac aagtccagtt tccatcgttg ctggtttacc agaacctgta
    85201 acagtagcac ctttgataga tggttgagtt tcagcaactg tcaactcaac agtagtagga
    85261 acggtgacac cgatcacttc agttccgtag aattggattt tcacatcaga gttttcaagg
    85321 atgtaaagca attcgttttc aacattgact acagggattt cgtattggtc ataagtttct
    85381 gtattcatga agtatgctgt gtcatccatt ttgtacaagt attgagctgg gacagtctcg
    85441 ataatagctt gttcaaattt ttcctctgga cggtagcttg tgtcaaatgt agaaccagta
    85501 cggacatcac gcaatttcat acgcatgatc gtgtttcctt tacctggttt gtggtgacta
    85561 gcttccaaaa cgcgaatcaa tttgccgtca gctgtttcaa aggtcatacc agcttttaat
    85621 ttacttgctt caatcatgtt tttacctctt taataaatat tattactctt cattttacca
    85681 taaaatactc tggaaataaa gccaaaatag ttattgggag ttggtagaga agaaaaaacc
    85741 aaccttaggg ctggttttgt tctgtctagt tttctttcaa cttcgccaat tcctgtgcaa
    85801 gtagtttaag ggcaacttgt gggttggctt ggcctttggt tgccttcata aggaatcctg
    85861 taaaggcctt gtcggcgtta cgtttgcctg acttgaagtc ggcaacagca gcttcgttat
    85921 cggcaaagac ttggtggatg attgggatca agatagctgg atctgaaatt tgaaccatac
    85981 ctgctttttc cacgtattca cgcgcgccac cgccattttt agctagatgg acaaagactt
    86041 tcttggcaat cttagatgaa atagtaccgt cttcgatgat ggcaatcatt tcaaccaagt
    86101 tttctggtgt caattcgatt tgttccagtg ttttaccttc agcattcaag aactgagcga
    86161 cttccccttg gagccagtta gagacttgtt tggcatcacc acctagggca acagcttttt
    86221 caaagaagtc agaagtgact ttattagcag tcaactgact agcatcgtag tctgataaac
    86281 caaggtcaga tacataacgc gcacgacgtt cttttggaaa ctctggcaac tcagtccgca
    86341 tttcctcaat ccactcgttt gagatttcaa agagaggcag gtctggttct gggaagtagc
    86401 ggtagtcagc agccccttcc ttgacacgca tgaggatggt tgctttgtta gcttcatcgt
    86461 aacggcgtgt ttcttggcgg atttgaccac ctgagcgaag tatttcagct tgacgttgga
    86521 cttcgtattc aagccccttg cgaacgtttg agaaggagtt gaggttcttc aattcagtct
    86581 tggtaccgaa tttctcttga ccataaggac gaagggagat gttggcatcc acacgcatcg
    86641 aaccttcctc catcttaacg tcagaaatgc cagcgtactg gataacttcc ttgagggctg
    86701 tcagataagc ataggcttct tcaggagaac gcatatctgc ctcagataca atctcaatca
    86761 agggaacccc ttggcggttg aggtcaacat aagagtaccc atctgtacca tgggtgtttt
    86821 taccagcgtc ttcctctagg tgggcacgtt cgataccgat tttcttggtc gtaccgtctt
    86881 ctagctcgac ttcaatccag ccgttataac cgattggctc atcaaactga gaaatttggt
    86941 aggctttggg gttatcagga tagaagtagt tcttgcggtc aaagtgcatc tttttgtgga
    87001 tgtccatgtt gagggcaaga gcagccttga taccggcatc aacaacccct ttattgagaa
    87061 ctggtagaac tcctgggaaa gaccagtcaa tcacgttagt gttggcattt tggtcatttc
    87121 caaagtgggc agaagtaggt gagaagattt ttgaattggt gttgagctct acgtggactt
    87181 caagtccgat gactgtttca aagttcatta gttgtcacct ccaaaaatca cgggttgttg
    87241 tttgtggtag tctgttgttg cttcaaaagc agcagcagct tggtaaatgg tttcctcaga
    87301 gtacttggga ccaatcaatt gcagaccgac aggtagacct tgagagaatc cagcaggaat
    87361 cgaaattcca gggagacctg ctaagtttac aggtatggtc aagaggtcag ccaagtacat
    87421 ggcaactggg tcatggttga gtgagtccaa gtcataggca acacttggag cagttggacc
    87481 caaaatcaaa tcgtaatccg cgaagacttt ttcgaaatct tgaatgatga gggtacggac
    87541 ttgaccagcc tttttgtagt aggcatcata gtaacctgat gaaagactga aagtacccag
    87601 catgatacga cgttttacct cttcaccaaa accttggctt cggctgttta catagatttc
    87661 atcaaggttg gttgcatctt ctgcgcgata gccgtaacgg ataccgtcga agcgttgcaa
    87721 gtttgatgaa gcttctgatg aagcgatgat gtagtatacg gcaactccgt atttagagtg
    87781 aggaaggctg acttcttcga caatagcacc caatttttca aagtgtttag cggcatttag
    87841 aatggtttcc ttaacctctg ggttaattcc ttcaccgagg tattccttag gcaaagcgat
    87901 tttcataccc ttgatgtctt ggccgatttt tgaagtaaag tcggcgatgc ggacaggagc
    87961 agaagtagag tctttagcat cttcgctggc aatagcgttg agcaagaggg cgttttcctt
    88021 aacagtagga gcaaaaggtc caatctggtc taatgagcta ccaaaggcaa tgagaccgaa
    88081 acgtgaaact gttccgtagg ttggtttgag accaacgatt ccgttgaagg cagcaggttg
    88141 gcggatggaa ccaccagtat cagaaccaag tgacaagcga acttgtcctg aggctacagc
    88201 tgcggcagaa ccacttgatg acccaccagg aaccttgctg tggtcccaag cgtttttagt
    88261 tgctccgtag tgtgaagttt cacctgaacc acccatagca aattcgtcca tgttggtctt
    88321 tccaacgaca atcatgccct tggtttttgc attggcaaca gctgtcgcat caaagattgg
    88381 ctcatagttg tagagcattt ttgaggcagc agttgtgaga ataccgtctg tagagatgtt
    88441 atccttaaca gcaagtggaa ttcctgaaag gacattgtca gcatcaattc cagcttcatc
    88501 aatggcttta gcttgaacaa gagcttgctc ctcagcgatg gtgacaaatg aattgagggc
    88561 ttcctcacga gacttgatat cttcaagtgt tgcttgggtc aattctgttg cagaaatttc
    88621 cttagaaaca aggagattgt gcaactcttc aatagtttta ttgttaaaag tcattaggca
    88681 tctcctccat cgtctaggat agctggcacc ttgatatagt agttgtcttt ttcaggtacg
    88741 tttttaaaca agcgatcacg gtctgttcct tcttcggcca catcagggcg gagtacagtc
    88801 ttgcggtcag ccatagtcgt agtaggtgcg acaccagttg tgtcaacttc gcccagcaat
    88861 tcaaccatgt caacaatctt agacaaggtg gtcgcaaagg cagcagtttc ttcttcagag
    88921 aatcttaatt ttgaaagatt ggcaacgtgt gttacctctt cttgcgtaat tttcatcttg
    88981 catcctttcg tgaaatgatg atttttagtc tgttctattt taccatattt tcctataaat
    89041 aagggagggg gaagtgaaaa gaaatagaaa ttgaaaaaaa tgctaaaatc cgatataatg
    89101 gagtgttgaa aggaatggta aaatccaatg taaaaatcat tctcagctat tgaaacaaga
    89161 aaaatagaga atcaaagaaa gagaacttat gaatattcaa gaagaaatta agaaacgtcg
    89221 tacctttgcc attatctccc acccggacgc ggggaaaaca accatcactg agcagttact
    89281 ctactttggg ggtgagattc gtgaggctgg tacggtaaaa gggaagaaaa cagggacttt
    89341 tgctaaatct gactggatgg atatcgagaa gcaacgtggg atttctgtta cttcatctgt
    89401 tatgcaattt gactacgacg gcaagcgcgt gaatatctta gacacgccag ggcacgagga
    89461 cttctcagaa gatacctatc gtaccttgat ggcggtggat gctgcggtca tggtcgtgga
    89521 ctctgccaag gggatcgagg ctcaaaccaa gaaattgttt gaggttgtga aacatcgtgg
    89581 cattccagtc tttaccttta tgaacaagct ggaccgtgac ggtcgtgagc ctttggatct
    89641 cttgcaagaa ttggaagaaa tcttgggcat tgctagctac cctatgaact ggcctatcgg
    89701 gatggggaaa gcctttgagg gcttgtatga cctctataac caacgtttag agctttacaa
    89761 aggggatgag cgttttgcca gtctagaaga cggggacaaa ctctttggta gcaatccttt
    89821 ctacgagcaa gtcaaggatg acattgagct tttaaatgaa gccgggaatg agttttcaga
    89881 ggaagctatt ctggctggag aattgacgcc tgtctttttc ggttcagccc tgacaaactt
    89941 tggtgtgcag accttccttg aaaccttcct caagtttgct ccagaaccac atggtcacaa
    90001 gaaaacagac ggtgaaattg tggatcctta tgacaaggat ttctcaggct ttgtctttaa
    90061 aatccaagcc aacatggacc ctcgtcaccg tgaccgtatt gcctttgtcc gtatcgtatc
    90121 tggcgaattt gagcgcggca tgagtgtcaa tctccctcgt actggtaagg gggctaaact
    90181 atctaatgtt acccagttta tggcggagag tcgtgagaat gtgaccaatg ccgtggcagg
    90241 tgatattatc ggggtttacg ataccggtac ttatcaggtt ggggatacct tgacggttgg
    90301 aaaaaacaag tttgaatttg aacccctgcc aacctttaca cctgagattt tcatgaaagt
    90361 ttctgctaag aatgttatga agcaaaaatc cttccacaag gggattgagc aattggtgca
    90421 agaaggagcc gttcagcttt ataagaatta ccaaacaggt gagtacatgc taggcgctgt
    90481 aggtcaactc cagtttgaag tctttaaaca ccgcatggaa ggcgagtaca atgcggaagt
    90541 agttatgaac ccaatgggta aaaagaccgt tcgttggatc aagcctgagg acttggatga
    90601 acggatgtcg tcaagtcgca atatcttagc caaagaccgt tttgaccaac cagtctttct
    90661 ctttgaaaat gactttgccc tccgctggtt tgcggacaag tatccagacg tagagttaga
    90721 ggagaagatg tgactcagta ccaacaattg gaactaaagt tccaaattgt tggacgctag
    90781 tcgctatttg gcgaactagc taactgcctc actaactacg tttggcaaat gaaatcgatt
    90841 tgtaaaactc cgtgtcgtaa tgtaaaaatt gtaaaccgta atgggacttc atctaaaatc
    90901 tccactggta tggttttacg aacgaatagg cgaaaataat ggttacgtag aaacatatga
    90961 tgggaaacat atgatgggaa taatatgatg ggaataaaaa gaaatgagat aaaaagtgaa
    91021 agaagagaaa aaagcgattg tcttaggtgc agataatgct tatatggata aagttgaaac
    91081 aacaataaaa tctctttgtg ttcatcatta taatcttaag ttttatgttt ttaatgatga
    91141 ccttccgcga gaatggttcc aattgatgga aaaaagacta gagactttga atagtgagat
    91201 tgtgaatgtt tagattgaca gtaatattct gaaaggttat cgtctcccct ttgagggact
    91261 ttcatatgcg gcttttttag gtattttatt ccaaagtatg taagcgagtc tcgtgtactg
    91321 tatttggatt cagatattgt tgtaagaaaa tcgatagatg agctgtggga tttggacttg
    91381 acggctatac ccttagctgc agttagagat gatttttata cacataattt taattctggt
    91441 gttctgttaa tcaacgatgg catgtggaga gcagaaaatg tcacgcaaga cctgatttaa
    91501 gacacaaata aaaaatcttc caattcc
//
LOCUS       DAVDWT010000004        74408 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVDWT010000004 DAVDWT010000000
VERSION     DAVDWT010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 74408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74408
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.004"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(77..1552)
                     /locus_tag="VUW50_000333"
     CDS             complement(77..1552)
                     /locus_tag="VUW50_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HET7982294.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLLIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVAFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            complement(1600..1905)
                     /locus_tag="VUW50_000334"
     CDS             complement(1600..1905)
                     /locus_tag="VUW50_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET7982295.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            complement(1943..2419)
                     /locus_tag="VUW50_000335"
     CDS             complement(1943..2419)
                     /locus_tag="VUW50_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7982296.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAETTA"
     gene            complement(3046..9465)
                     /locus_tag="VUW50_000336"
     CDS             complement(3046..9465)
                     /locus_tag="VUW50_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7982297.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALSISDKEKV
                     AENKEKHEDIHSAMETSKDTEEKKATVIEEKEVVSKNPVIDTKTSNEEARIKEDSNQS
                     QGDNAHSSANKGTENPKKEDRLVYIAEFKDKESGEKAIKELSSLKNTKVLYTYDRIFN
                     GSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSINAPFGK
                     NFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKIPHAFN
                     YYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIFSYK
                     MYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAGI
                     PMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFDK
                     VNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDLRGKIA
                     VMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKSQVF
                     SISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKEI
                     DFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKSTY
                     GYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQNTARP
                     MMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSISLKE
                     IKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGKQIVPE
                     IHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEEMEA
                     LNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGKPKIPG
                     TLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSGSKIAN
                     IYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVISREHFI
                     RGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIRGQF
                     EPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTYH
                     KVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGEGQG
                     RNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFADHADEKGMISYY
                     LVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTEVEHHHQENEESIKEKSSFT
                     IDRNISTIRDFENKDLKKLIKKKFREVEDFTSETGKRKQEYDYKYDDKGNIIAYDDES
                     ALEYETEKFDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKFIEIKATK
                     YDSHSKTMTFDLYANINDIVDGLAFAGDMRFFVKDDDRIKAETKIRMPEKNKETKAEY
                     PYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYALKV
                     TTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQAVLM
                     SALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKSNVEA
                     IKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELRVN
                     ESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGNVYL
                     QTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNGFD
                     GEIQEQHIYINGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTGEVSE
                     LKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGYV
                     INLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREDH
                     SQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFLG
                     LKKKNQD"
     gene            9686..9772
                     /locus_tag="VUW50_000337"
                     /pseudo
     CDS             9686..9772
                     /locus_tag="VUW50_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(10153..10350)
                     /locus_tag="VUW50_000338"
     CDS             complement(10153..10350)
                     /locus_tag="VUW50_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7982298.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNPQNPCQILIKKLE"
     gene            complement(10335..10613)
                     /locus_tag="VUW50_000339"
     CDS             complement(10335..10613)
                     /locus_tag="VUW50_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HET7982299.1"
                     /translation="MFVIEEVKDENQKKTVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTGFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            complement(10652..11437)
                     /locus_tag="VUW50_000340"
     CDS             complement(10652..11437)
                     /locus_tag="VUW50_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7982300.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            complement(11439..12257)
                     /locus_tag="VUW50_000341"
     CDS             complement(11439..12257)
                     /locus_tag="VUW50_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET7982301.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            complement(12259..13251)
                     /locus_tag="VUW50_000342"
     CDS             complement(12259..13251)
                     /locus_tag="VUW50_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7982302.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            13576..13836
                     /locus_tag="VUW50_000343"
     CDS             13576..13836
                     /locus_tag="VUW50_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HET7982303.1"
                     /translation="MSTLTRDRVYNFCVNTQLFEEAKTILESKNISLSDALNLFVEQI
                     VLEKGLTIKTADQPKAELDKGYQDIIEGRLYDADEVFSKYGL"
     gene            13826..14194
                     /locus_tag="VUW50_000344"
     CDS             13826..14194
                     /locus_tag="VUW50_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000385025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET7982304.1"
                     /translation="MDYKVRFTEQARQDLDDIFIYYSTEFSENIAKKVITRLQQITNL
                     LIFSPEGGISFDHKIGYSLYPENMLRMIVSKQYLLFYLIHEKEVHTLRIINSRTDYLN
                     QLDHLFRTCRKLFSSQIISL"
     gene            complement(14766..15128)
                     /locus_tag="VUW50_000345"
     CDS             complement(14766..15128)
                     /locus_tag="VUW50_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HET7982305.1"
                     /translation="MLEVGLNSLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
     gene            15142..15288
                     /locus_tag="VUW50_000346"
     CDS             15142..15288
                     /locus_tag="VUW50_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982306.1"
                     /translation="MKTKEQTRKLATGCSKHCFEVVDRTDVVSNHTHGKATLTWFEEI
                     FKEY"
     gene            complement(15880..16569)
                     /gene="rplA"
                     /locus_tag="VUW50_000347"
     CDS             complement(15880..16569)
                     /gene="rplA"
                     /locus_tag="VUW50_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET7982307.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(16777..17202)
                     /gene="rplK"
                     /locus_tag="VUW50_000348"
     CDS             complement(16777..17202)
                     /gene="rplK"
                     /locus_tag="VUW50_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET7982308.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            complement(17511..18227)
                     /locus_tag="VUW50_000349"
     CDS             complement(17511..18227)
                     /locus_tag="VUW50_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET7982309.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKTKNEDGETKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(18230..18700)
                     /locus_tag="VUW50_000350"
     CDS             complement(18230..18700)
                     /locus_tag="VUW50_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET7982310.1"
                     /translation="MCLICQRIDLIKKEENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVTELHHLKKETRLRFLEEMSLVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     TGDMNDHGLKGRGPVWWVPFEEMTAETCQAKPDEIKRLVKRLSSEVDKLLEIKE"
     gene            complement(18702..19739)
                     /locus_tag="VUW50_000351"
     CDS             complement(18702..19739)
                     /locus_tag="VUW50_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET7982311.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSGLVD"
     gene            complement(19943..21268)
                     /gene="brnQ"
                     /locus_tag="VUW50_000352"
     CDS             complement(19943..21268)
                     /gene="brnQ"
                     /locus_tag="VUW50_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HET7982312.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(21439..21531)
                     /locus_tag="VUW50_000353"
     CDS             complement(21439..21531)
                     /locus_tag="VUW50_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7982313.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(21574..21726)
                     /locus_tag="VUW50_000354"
     CDS             complement(21574..21726)
                     /locus_tag="VUW50_000354"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7982314.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LTDSYD"
     gene            complement(21886..22464)
                     /locus_tag="VUW50_000355"
     CDS             complement(21886..22464)
                     /locus_tag="VUW50_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET7982315.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(22518..23918)
                     /gene="pepV"
                     /locus_tag="VUW50_000356"
     CDS             complement(22518..23918)
                     /gene="pepV"
                     /locus_tag="VUW50_000356"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HET7982316.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(23931..24536)
                     /locus_tag="VUW50_000357"
     CDS             complement(23931..24536)
                     /locus_tag="VUW50_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET7982317.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYSEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            24707..25507
                     /locus_tag="VUW50_000358"
     CDS             24707..25507
                     /locus_tag="VUW50_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7982318.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIVFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(25689..26522)
                     /locus_tag="VUW50_000359"
     CDS             complement(25689..26522)
                     /locus_tag="VUW50_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET7982319.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEALDGQYGLEDPQEVQLLRMTQFLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELLNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRREDNYQGHNLELLASLIEKHGYVEDVKDFFDF
                     L"
     gene            complement(26512..28356)
                     /gene="uvrC"
                     /locus_tag="VUW50_000360"
     CDS             complement(26512..28356)
                     /gene="uvrC"
                     /locus_tag="VUW50_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET7982320.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDETYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            complement(28396..29097)
                     /locus_tag="VUW50_000361"
     CDS             complement(28396..29097)
                     /locus_tag="VUW50_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET7982321.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(29176..30408)
                     /gene="murN"
                     /locus_tag="VUW50_000362"
     CDS             complement(29176..30408)
                     /gene="murN"
                     /locus_tag="VUW50_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HET7982322.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEEKLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(30413..31633)
                     /gene="murM"
                     /locus_tag="VUW50_000363"
     CDS             complement(30413..31633)
                     /gene="murM"
                     /locus_tag="VUW50_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HET7982323.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(31745..32524)
                     /locus_tag="VUW50_000364"
     CDS             complement(31745..32524)
                     /locus_tag="VUW50_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HET7982324.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYMALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(32593..34254)
                     /locus_tag="VUW50_000365"
     CDS             complement(32593..34254)
                     /locus_tag="VUW50_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET7982325.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(34429..36651)
                     /gene="recJ"
                     /locus_tag="VUW50_000366"
     CDS             complement(34429..36651)
                     /gene="recJ"
                     /locus_tag="VUW50_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET7982326.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSIPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPYRDLFIAFGGHAGAAGMTLEVEQLSDLSQILEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEVSFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMMDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            36928..37686
                     /locus_tag="VUW50_000367"
     CDS             36928..37686
                     /locus_tag="VUW50_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET7982327.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAKKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            37726..38490
                     /locus_tag="VUW50_000368"
     CDS             37726..38490
                     /locus_tag="VUW50_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET7982328.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTALLDDSFKPSDYSIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            38503..39180
                     /locus_tag="VUW50_000369"
     CDS             38503..39180
                     /locus_tag="VUW50_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET7982329.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISALRLIILPQAFRIILLPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPGFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            39190..39849
                     /locus_tag="VUW50_000370"
     CDS             39190..39849
                     /locus_tag="VUW50_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET7982330.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPILRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            40144..41334
                     /locus_tag="VUW50_000371"
     CDS             40144..41334
                     /locus_tag="VUW50_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HET7982331.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGNKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(41655..42536)
                     /locus_tag="VUW50_000372"
     CDS             complement(41655..42536)
                     /locus_tag="VUW50_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HET7982332.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYDDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(42677..44005)
                     /gene="vncS"
                     /locus_tag="VUW50_000373"
     CDS             complement(42677..44005)
                     /gene="vncS"
                     /locus_tag="VUW50_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HET7982333.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGATMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTLRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIAFPKVAQD"
     gene            complement(44002..44658)
                     /gene="vncR"
                     /locus_tag="VUW50_000374"
     CDS             complement(44002..44658)
                     /gene="vncR"
                     /locus_tag="VUW50_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HET7982334.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASIAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(44707..44793)
                     /gene="pep27"
                     /locus_tag="VUW50_000375"
     CDS             complement(44707..44793)
                     /gene="pep27"
                     /locus_tag="VUW50_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HET7982335.1"
                     /translation="MYEKGFHNVLSSGQLLTDKRPARDYNRK"
     gene            complement(44969..46348)
                     /gene="vex3"
                     /locus_tag="VUW50_000376"
     CDS             complement(44969..46348)
                     /gene="vex3"
                     /locus_tag="VUW50_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HET7982336.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMVLASSNLLRKQ
                     PKELLLDGE"
     gene            complement(46400..47047)
                     /gene="vex2"
                     /locus_tag="VUW50_000377"
     CDS             complement(46400..47047)
                     /gene="vex2"
                     /locus_tag="VUW50_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HET7982337.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(47060..48337)
                     /locus_tag="VUW50_000378"
     CDS             complement(47060..48337)
                     /locus_tag="VUW50_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7982338.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            complement(48474..48758)
                     /locus_tag="VUW50_000379"
                     /pseudo
     CDS             complement(48474..48758)
                     /locus_tag="VUW50_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(48901..49500)
                     /locus_tag="VUW50_000380"
     CDS             complement(48901..49500)
                     /locus_tag="VUW50_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982339.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNSSLYTTIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
     gene            complement(49637..50521)
                     /locus_tag="VUW50_000381"
     CDS             complement(49637..50521)
                     /locus_tag="VUW50_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HET7982340.1"
                     /translation="MREKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGSQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     HVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(50525..50911)
                     /locus_tag="VUW50_000382"
     CDS             complement(50525..50911)
                     /locus_tag="VUW50_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HET7982341.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(50904..52247)
                     /locus_tag="VUW50_000383"
     CDS             complement(50904..52247)
                     /locus_tag="VUW50_000383"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET7982342.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(52334..53218)
                     /locus_tag="VUW50_000384"
     CDS             complement(52334..53218)
                     /locus_tag="VUW50_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7982343.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(53230..53847)
                     /gene="cysE"
                     /locus_tag="VUW50_000385"
     CDS             complement(53230..53847)
                     /gene="cysE"
                     /locus_tag="VUW50_000385"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HET7982344.1"
                     /translation="MGWWHETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KYDFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(53863..56076)
                     /gene="pnp"
                     /locus_tag="VUW50_000386"
     CDS             complement(53863..56076)
                     /gene="pnp"
                     /locus_tag="VUW50_000386"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET7982345.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMTGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            complement(56761..57627)
                     /gene="metF"
                     /locus_tag="VUW50_000387"
     CDS             complement(56761..57627)
                     /gene="metF"
                     /locus_tag="VUW50_000387"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET7982346.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(57691..59940)
                     /gene="metE"
                     /locus_tag="VUW50_000388"
     CDS             complement(57691..59940)
                     /gene="metE"
                     /locus_tag="VUW50_000388"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET7982347.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLTVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEVAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(60201..60467)
                     /locus_tag="VUW50_000389"
     CDS             complement(60201..60467)
                     /locus_tag="VUW50_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7982348.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            complement(60551..61040)
                     /locus_tag="VUW50_000390"
                     /pseudo
     CDS             complement(60551..61040)
                     /locus_tag="VUW50_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            61196..62197
                     /locus_tag="VUW50_000391"
     CDS             61196..62197
                     /locus_tag="VUW50_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET7982349.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTDSGIAPVTVIVKKSYDFYTKAETFHTTRFGTIVLHSRKQDIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            complement(62265..64667)
                     /locus_tag="VUW50_000392"
     CDS             complement(62265..64667)
                     /locus_tag="VUW50_000392"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET7982350.1"
                     /translation="MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPGARIADNYK
                     IKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
                     IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKA
                     PYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNF
                     EGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKSS
                     RVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGAT
                     VRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTV
                     SVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAEG
                     AGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNVA
                     RKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAK
                     GILEALFTRLGLEVTYTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIPE
                     TYVAELNLSAIEGALQPAVPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVKR
                     LTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNGE
                     VR"
     gene            complement(64744..65253)
                     /locus_tag="VUW50_000393"
     CDS             complement(64744..65253)
                     /locus_tag="VUW50_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7982351.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTVSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            complement(65253..66299)
                     /gene="pheS"
                     /locus_tag="VUW50_000394"
     CDS             complement(65253..66299)
                     /gene="pheS"
                     /locus_tag="VUW50_000394"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET7982352.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            complement(66891..68306)
                     /locus_tag="VUW50_000395"
     CDS             complement(66891..68306)
                     /locus_tag="VUW50_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET7982353.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(68319..70157)
                     /locus_tag="VUW50_000396"
     CDS             complement(68319..70157)
                     /locus_tag="VUW50_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET7982354.1"
                     /translation="MSYKDKVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVIPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     DVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(70175..71014)
                     /locus_tag="VUW50_000397"
     CDS             complement(70175..71014)
                     /locus_tag="VUW50_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HET7982355.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKIGDKVNPIDI
                     EKSFFLKNRDDMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGVIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            complement(71314..73993)
                     /locus_tag="VUW50_000398"
                     /pseudo
     CDS             complement(71314..73993)
                     /locus_tag="VUW50_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            74242..>74408
                     /locus_tag="VUW50_000399"
     CDS             74242..>74408
                     /locus_tag="VUW50_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7982356.1"
                     /translation="MKLTYDDKVQIYELRKQGYILEKLSNKFGINNSNLRYMIKLIDR
                     YGIEFVKKGKNR"
BASE COUNT        21717 a        16390 c        13170 g        23131 t
ORIGIN      
        1 gtgtttttag caacctgcgg ctagcttcct agtttgctct ttgattttca ttgagtttat
       61 attttatagg agcgcattat tttgcttttg ctgcgtactc ttcgttacgt ttgatcattt
      121 gttttctgta ccaagcaaag ataccgatat agaatacaag gaaggctact gcaccaagga
      181 ttgctttgat atcaccagtt gtagtgttac caattgtcca accaagaagt ttttcgattg
      241 gtccttcaag agtagagtga gtaatcaatt gagtttggct cacaccttct gggaaggcac
      301 ctacaccttt agcaagttct gttgcaaatg gtgcaataag tgtacctgaa agaaggaaga
      361 gtggcaacaa gagtgttccg aagataatca tacggagcaa tttaccacga gttacaacca
      421 agagagctgg agtaacaccc atagcgatga tacctgcaag tggcaagata ccatttccaa
      481 cttttgaaag aagcactgct tcaatcaaca tgattggtgc aagtacgttg gcacaagccc
      541 agatttcagc acgaccagcg atgaatggcc agtcaagacc gatattgaat ttacgtcctt
      601 gaagacgttt agtagcaacg tttgtaatac cttgtgatag tggttctacg gctgcgatga
      661 accatgaacc gataagtgag aagagttcca aagatacacc ggcagtcaaa ccaagagaca
      721 accatccttt gataacaaga cgccatttat ctgcatctgc aacacctgca attggatgtg
      781 gagttcccat aataccgata acgataccaa ggatgaaacc gatgaagaat ttagatcccc
      841 agaaaccgat tttcttgttc aatttagcag catcaaagtc atatttatca aggcctggga
      901 agaatttttc aaaaatctta tccaaaacca tgataactgg gttcatcatg tagttcatgt
      961 gagttgatgt cattggtgat gaacttgggg cgttaagaag gtcatcaaat gtaggtttca
     1021 tcaagtcaga gttgataatt ttcaacacac cgacaagaac gatagctgct gtagcaatca
     1081 agagtgaaac cccttgactc acaccattgt tatcagcata ccatttaatc aagagacctg
     1141 tgatagacaa gtgccagata tcaaagatat cgacatcaag tgtatcggtt ttcttcatag
     1201 caagcatcac tacgttgaca atcaacatga tgagcaagaa gtaaagtgtc caagcagaac
     1261 cccaagtgat tgttgcaagt ggtgcccaac caacgtcggt aatactcaat tggataccag
     1321 tgttttcaac gaattttgca agtgatgctg agaaagcacc atttagcata ccgatgatag
     1381 caccgatacc tgtaagagcg atggcaagtt tgataccacc ttcaagcgct ttggagaatt
     1441 tcactccaaa aagtaaagcc aatactgtca aaatgattaa catgatgaca ggtccaccca
     1501 tttctaagat gggattgaaa acctttccga ttaggtcaaa gattgcatcc ataacagttc
     1561 ctcccttttt tatgttatat gaatgttaac aaattagaat tagcttaatc cgtgttcttt
     1621 aatagctgct tcaatattgt caaatactgg agcgctcatt gctgggatac ggaataagat
     1681 tggtccagct tcgataactg ggatacctgg ttcaaaacca aggtctgttg cagcgattgg
     1741 tgtaaagata tcgtaacctt tcataaggtc ttcgtttaca tctttcacca tgactgcatc
     1801 acagtgaaca tcataaccac ggtttgaaag ttcttcttct agagcacttt taatttggtg
     1861 acttgagtta acacctgcac cgcaggcagc aagaatttta atcatttaga tttcctccga
     1921 ttttattttt taatagacaa gattaagcgg ttgtttcagc aatgtaagca taaagttttt
     1981 ctggttcgga aatttttgat aggtcttcaa gatgtccatt tcctgtgaaa aagtccatca
     2041 actgagccag aatatttgtt tgacttgaac ttgagttatt gatgataaag aagagcaagg
     2101 atacttctac ttccttatca ggagctatca tattgtgaaa agttactggt ttttctaatc
     2161 gaacaaccac cactttctca gctagattat gaacaatatc tgtatgagga atcgctacat
     2221 ttggcaagtc ctttcctaga aattccatat ctaaaccagt tggaaatgac ttttcacgcg
     2281 tgatcaaagc ttcacgataa gttggagtga caatttctcg ttcttccaat aaagttgcaa
     2341 cctgatcaaa gagttgttct tgattatccg cttctaagca aaacacaagg tttttgtcaa
     2401 agaaataatc taataccata agtttttccc ttctttccat taactttatg ccataagtat
     2461 aacactatat gaaaccgttg tcaattactt tctgttcttt tttgtctctt tttttatatt
     2521 tttgttttgt ttatagtttg ttatataaaa ataaacacac aaataaatac tccaagcatt
     2581 ttttctgttc taatactcaa tgaaaatcaa agagtaaact aggaagctag ccgcaggttg
     2641 ctcaaaacac agttttgagg ttgtagataa gactgacgaa gtcagtgatc atatcgtaac
     2701 cgtccaatag acttcgttat tgggcggtta cgaaaaactt ccttccaaat aaaaaggaat
     2761 taagcaacca aacgctactt aattccttga aattgtctca tcgcctctat tagcctttaa
     2821 attttgatag actctatttt ctaatcaata tgaccaatat tgtcaatact aaccctttca
     2881 atcaattcat tagttatcta aactaagtcc attaataact ccaaatagta tcaatagtga
     2941 agtaaactaa ataattgagt tataaatggc tgttttcaca aaactatgtc atcacctcac
     3001 tatctaatct cagtacataa accatttttt tctatagctt ttatcttaat cttgattttt
     3061 tttcttcaat ccaagaaaaa ttcctactat aaacattatt ccggcagcta atagtgtctg
     3121 ggctccgctt gctgttccag tttttggcaa cttattagga ttgttagtag ttgatttact
     3181 actgatattg tttttatcaa gaactgtagc tgtaacatcc ttagttgaat cagatttttg
     3241 tgaatgatct tctctttgta aatcctcttt tctgtgactt tcatttaatt gactatggtt
     3301 tacttgtggg ttatctttct ttttcgatac atcaaaagta ggtttatttt cttcctcctt
     3361 tttctcctct attttcttga atacaggttt tataaaggta tcttttgata gattaataac
     3421 atagccagcg tcttttttac cttcgaaacc agaaatttcc caaccatcaa attggtatcc
     3481 tttttctaat tcacccttat aaacaggtaa aataaaatct tcttccgaca ctatcgttga
     3541 actcatttct tttccatttt gaatggtcac agttacccta tgaggtttta attcactgac
     3601 ctctcccgta tctttattta aaatgaattc ttttacggtt gtatttcttg caaaatcttt
     3661 tacaacaatc ttaatgttta gtgtcttgtc tgttatttgt ttaatatcat caaacgatgt
     3721 atattctttt ccatttatat aaatatgttg ttcttgaatc tcaccatcga atccattatt
     3781 tcttttatca ttgatgttaa agactacaga ttttccatca gcatattcga tactagtatt
     3841 tcccttagga tcaatgttta cttcgggttt aacattatca tataaaaact gatagtggac
     3901 tccaactgct ttagcattca aatcgctata gccagtttga agataaacat ttccatccat
     3961 atctgttacc ttatctggaa atccgtttgc tttatagtct ttcattcccc agtccatgat
     4021 gtcaccgtct ttaacattca acttaatatt aaaatctcta gtgttatcaa tgtgtaaatc
     4081 tccgtagatt aaataattat ctacaaccga ttcattaact ctcaattccc agttaaaacc
     4141 acccttatca gaaatcttac ctcttaaata aaattctgga tttcgtacat aaattttatt
     4201 agatttagat ggattaaagt agttcttatc cattgaaagg tttactggtt tggtatcaat
     4261 aaataacatg gagccatctt cttttatagc ttctacattg gacttatcct ctccagtgta
     4321 ttctttatca tccttaccaa ataatacaag tttagaagaa tctgtcacaa gatttccgtc
     4381 tttatcgata gcttcccctt tatcgttcat tttaaatgta aacacttgat accttataat
     4441 gttaaagccg tccaaagccg acattaatac agcttgggta cttcttccat cttcaacatt
     4501 tctactatca gcataaattg ttgtttctga aaggactctt agattaggat tggccttttg
     4561 tatttttgct atatcttcct tgctatagac tccatttcct tctaacatat ccgtttttcc
     4621 aggattatag gtagtcactt ttagtgcata gccttttctt agaatgatgt tatcctttaa
     4681 cagatattgt tgtttttctg aatcagaata gattttacca gattccattt tagttaaatt
     4741 gtctggtttg ttttttgaaa ggtctccttc tcctaattcg attacattcc cataacttga
     4801 tacataggga tattctgctt tagtttcctt atttttttca ggcattctaa ttttagtttc
     4861 agcttttatt cgatcatcat ctttaacaaa aaaccgcata tctccagcaa aagctaatcc
     4921 atccacaata tcattaatat tagcgtataa atcaaatgtc atcgtttttg aatgtgagtc
     4981 atacttagtc gctttgattt ctataaattt atagttattt ccataatata ccttggcatt
     5041 tttagaaaca ttgcttatct ttccaagaat ttcaaagtgt ccatctttag atgggcttag
     5101 aacaccataa atttttgatt ttatttcgtc aaatttttca gtttcatatt ctagagcact
     5161 ttcatcgtca taagcaatga tatttccctt atcatcgtat ttataatcgt attcttgttt
     5221 cctcttacca gtttcacttg taaagtcctc aacttctcta aatttctttt taatgagttt
     5281 ctttaagtct ttattttcaa agtctctaat tgttgaaata tttctatcaa tagtaaaact
     5341 agatttttct ttaatagact cttcattttc ttgatgatga tgttctacct cagttgtatc
     5401 tttttttaga ctaccctctt ttccatttcc taaattttta aatttagatt ctgcaatctc
     5461 gccaagcttt tgatattttg atgaatcttg atcaggatct actaggtaat aagaaatcat
     5521 ccccttttca tcagcatgat cagcaaattt aattctatga atctttgtga aattgctaga
     5581 accatctaat gcaatgactt caatgatttt tcctcttaaa tctcctgcac ctttaatttc
     5641 ataaatggta tttccgtctt tatcaagttt tctatttctt ccttgaccct cacctgcgta
     5701 agttacttca agattttttt caacctctcc atcttcatta acaagagcgg cgccagcata
     5761 ccaaacttcg ttcgcaatct cgtcaaattt ttcaggatgt tctttttgat ctctcgcaaa
     5821 tagcgtttca ttcttatact gatcttttac cttatgataa gtatcctttg taatcaactt
     5881 aattttttca ggatttgaaa aatcaaccga aacaatctta ggggcggtgt tatcaatttt
     5941 tacaggaata taggaaacct gccatgggta atctttagtt aatctatatt taaatttata
     6001 gaaatattga ccttccgcaa tcggttcaaa ttgacctctt atcttagtag caggatcttg
     6061 attatcctta ctttctggtg cattttcttc tctacctcta ggattataga tgagtccatc
     6121 ccacttcaag tcaccccaaa cttttagttt agatgatttg attccctttg catcattgct
     6181 tttagaattt aaaattcctc taataaagtg ttctctcgaa atgactttta agtctctttg
     6241 attttctccc tctttatttg tatttactat tgaaatcaat ccttcttctg cacttcttaa
     6301 tacaagtgga gaaggtgtta atcctctttc aagttgagca tcttgaggat ttccatttga
     6361 atctaaagga taaatgttag caatcttaga accacttgat gatggagcgt tgatcccttg
     6421 gttattgaaa gcaaataatt ctggattttg atccagggat gttgtatttt tatcttttct
     6481 attttgtata actcctgctg gattaaattt atctatacca tgttctccac caattccctt
     6541 atttaaggtt cctggaattt tcggtttacc atcatcatca taacctccca gtgtttttga
     6601 tcttgaccct tcttcccaag cccatttatc aaggattggt tcgtggttcc aattcccagc
     6661 aaatcccatt agaggcatcg acaaagaagg ttggaagttt attttcttcc cgttggagtt
     6721 tagagcttcc atttcttcca ctgactcaaa atgaataaat gattctacaa atttattttt
     6781 gtttttggcc tctccaacat ttataaccgc attcaaatca aagctagaat ttgcgcctat
     6841 agtgaaagta tcatgctcaa atgtgatatt tgctcctttg actttttctg ggtgaatttc
     6901 tggaacaatt tgcttaccat ctggagattt ttcatcttta tatgtttcat caagttttaa
     6961 tctgtcagtt aaagaatctg tagttatcgc tgatgctgaa actttaaaag tcaaaggtct
     7021 gtttgatgta ttgtgaagct tgattgtaaa gtatttttta tcacctttta tttctttaag
     7081 agaaatggaa ccatatgagt ttaccaaacc tttagaatca gtgtttttga aagttgctac
     7141 aacttcattt ctcaaagcat tggccacatt aattaggcct gctccctgtt gtctaggtga
     7201 tgcaaagtat tgactttttt ctttccaaga agttgcatcc atcataggtc gcgcagtatt
     7261 ttgtagggca atttttgtaa gacttgtaag gtctattttg tcatctccct taagattttt
     7321 caatacaggt ctttcaagca tttcctttaa tttcggtcta atcaaaacag tagaagctgc
     7381 cacgattgga gtcgccatac tagttcctga catatagcca taagttgatt tgccattaat
     7441 aacattaagc gtggatttaa tatttttacc aggtgctgaa acatcgggtt ttaaaagtaa
     7501 atctattctt ggcccccaag atgtagatcc tgctggaacg atgatatctt ctttatagag
     7561 ttctttgtct gtgtcaggtg caaacttaaa gtcaatctct ttttcgtcac ctacattcgg
     7621 tttgttggaa ttaaaacttt ccatatcaat tggatagtat tgctccaatt tatctttaaa
     7681 atcttcttta ttatttcttt tgacttcagt ttttttatta ggattaatca tgttccatag
     7741 ctttacacca tcatctcctg aaattgaaaa cacttgactt ttagtacctt catccgcttc
     7801 atatcccata gctggaagct ctgtccaatt atctctattg tagtaattta cagtatttac
     7861 aaccataatg gcgcgtgcac ccttatccat agctttttta aaagcatttt ttaaatcctt
     7921 tgtataaatt ctatccatta ctgcaatttt gcccctaaga tccaaaccta tcaaatcttg
     7981 gtcttgcccc ttgcctatat atacaaattt taatttacta ggagcttttg ttccatcttc
     8041 atttgttgtg attttattct tatcgaaaaa ggcccctata tttctgtatt taaaactttc
     8101 tccacctatg ttaactttat caaactcaac tgtttgattt ttagcagaag cgaccgctat
     8161 agcatcttca tgtgctgcag ttcgtgttac atttccagtg tcggtcattt tcagatgatt
     8221 atttgctact aaatcccatg aagaacttga agcagaagtc gcatagttac ccgtagcgac
     8281 aaccattgga atgcctgctt ttcttaatgc ccgaatagct tgccaatatt tctcacctac
     8341 aagacctgtt cctgtaaaac cagatgatac cgaaacaaca tcaacgttgt gtttgataga
     8401 atcttcaata gcatgaaaca ttgtttcatc acccgcaaac ccagatcctg cgtcagaata
     8461 cattttgtaa gagaaaattt gtgcattagg tgcaattcca tctatgccgt taaagttttt
     8521 gatatcttgt tcagtatcat ttccagcaag aatccctgca atatgcatcc catgtgggtc
     8581 aaaataatcc cttccatcat catatttttc tacagtgatt ttgccaccat tataataatt
     8641 gaacgcatga gggattttat cactcaacca ataattttta tcagttcctt ttaagtcttc
     8701 ttttttaaat ctcattgagg ctttggcatc atcatcgatt ctcatagcct tatgtctata
     8761 atctgttcca gtatcgatat ttgaaatgac catacctcta ccatcaaaat ttttcccaaa
     8821 tggagcattg atagacttta ggtaatcaat agcttcctca actccaattt cctttctggc
     8881 atgattcatc atgggttgga ctttttgtgc cctttcaacc gatgaaatac cttctatttg
     8941 tttaattttg tccaagttat ctggagttgt ttctatggca ctaccgttaa aaattctatc
     9001 ataagtatat aaaacttttg tattcttaag actggatagt tccttgattg ctttttctcc
     9061 agattcttta tctttaaatt cagcaatata gacaagtcta tcttcttttt tgggattttc
     9121 tgtgccttta tttgcagacg agtgtgcatt atctccttgg gattgattgg aatcttcttt
     9181 gattcttgct tcttcattgc tagttttagt gtctatcaca ggatttttac taacaacttc
     9241 tttttcctca ataactgttg ctttcttttc ttcagtatcc tttgaagttt ccatagcact
     9301 atggatatct tcatgtttct ctttattttc tgctactttt tctttatcag agatgcttaa
     9361 agcatcttca gagctagatg tgtctgctaa gactacctca ttaggggcat aggctgctaa
     9421 aataactgca gctgtggtta atgacaatac tgtacttttt ttcattttaa ttccttacat
     9481 atttatataa cttccaatag ataataaact ttaactttgc tagcctttgt tataaaaagt
     9541 tttactaagt attatctagg aaatagagta gtacatttat atataattgt tatctctcca
     9601 taaaaacagt atatcatttt aaaaaattta attcaagaaa aattaacctg cattaattta
     9661 ttttttagca cacgttaaaa aataagatta gtactcaatg aaaatcaaag agcaaactag
     9721 gaaactagcc gcaggttgct caaaacactg ttttgaggtt gtggatagaa ctgacgtaat
     9781 cagtaaccat acgcacggca aggcgacgtt gacgtggttt gaagagattt tctaagagta
     9841 taagtccaaa actttcgtta accgggatac attataagca cattcatacc atcaaaaata
     9901 gtagcaataa acttgaacaa ctaccagaaa cacgaaaaca ggcataattg atgaaatatc
     9961 agcgctatta aacaatataa ataaccgcta agaataaggc ttaacggtta tcttctacct
    10021 atctattttt tcaactcctc agtcatttat ctacctataa cctaggcttt acgagtgcct
    10081 tgctctacca actaagctaa atcggcgatt acacttacag tatggcactc tgagaataga
    10141 tgtcaagtaa tattattcaa gctttttaat caaaatctga caaggatttt gcggattcca
    10201 tagctgagaa aaaatctcta actttttaaa gccaagacct cgataaaagt catttgttcg
    10261 atcataatct ttattagaac cttctgccac tgttttgacc tgcagataac caactttttt
    10321 acgagcttca ctctctaaag tagcaagcaa ttggctccca atttttctac cttgataagc
    10381 ttttttacgc cgagacaatc aatctctgca caatcttcac tcgaatagga taagcttaca
    10441 aatccagtca aatcactctc ctgataggcg gtccaaactt gcagtgtcgt ggttccttct
    10501 atataggctt gtgtgctttc tgggattcca aaccattctg gcaaatcctt caaaacctca
    10561 gctacaactg tctttttttg attttcatcc ttgacttctt caattacaaa catcataagt
    10621 ttctttcttg attttagtct tactttagct tttacgaacc cgcactttcg taggaatcta
    10681 agcccttatc ccaaagttta agggaaataa caaagaaaac cagagaaatc aacatcaaac
    10741 ctcctacgtt aaagaacaca tccttttcct gtaagaaata gctagctgga tagtaggctg
    10801 taaaggcgaa aggcacgata aagctaatca accaacgaag aagagaattg taaatagaaa
    10861 tcggatactt agcaaagtca ttgaacatat agaagatgta aatcatggcg cctgactgct
    10921 tagtccaaaa ggcgatactg gctgttgcga ttttaagaga agtataaatc aaggtcgcaa
    10981 aaggaataca aactaggaaa agcaggaatt ttggaagagt ccaaacaatg ctggtcactg
    11041 ttgttcccaa taaaatacca ccgactaaga gttcacccaa ggcatcaatc tgaaaggttt
    11101 caactaggat gtgaaagaga ggattgatgg gacgagtcag atacttgtca aactcccctt
    11161 ttcggactag gcgttgccct agtgcccaga gattgtcaaa aaagagatgg tccattccct
    11221 tgggaatcaa ggaaaatcca taaatgaaag ctatctcttg aaaggtccag ccttctagga
    11281 atggaatatg ttgaaagatg acattgagaa acaagagatt caagccttga gccagaaaga
    11341 ctcccaagac accaaccaca aaatccacct tatattccat gatttgtttg atgtattgtc
    11401 tgataaaaat cagatgcatt cgttgatatt ttttcatact aacctccttg aatggtgata
    11461 aaggactgga cccgtttcca aatcaactga gacaatccca ccatcactaa gagccagaag
    11521 aactgcaaaa ggagtgcctg aagaatctga ctggcatcgt attttccaac aatgatcata
    11581 actggagtat aaatcaagga tgaaaaaggc aaaaaggaaa gaatatctga aacaaccttt
    11641 ggaaagaatg ccaagggaat caaactcccc gacataaaag ccactatgga agtcttaagt
    11701 aggttggaac cccaaagatt tttaaacaca aaggctgaaa atccaaagca aatattaaag
    11761 aaaaagttaa tcagataggc gagcgttaag ctaaaaagat aaaggacagt taatcctagc
    11821 acctctacaa taccttgacc cgatatgatt ttcatcaaga caatgacact taaaaacgga
    11881 aggccaacgc tgataaaaat caaccacttg gaaccaagct cggtgaaaag ataggaggcc
    11941 gcaaaatgca ctggtcgcaa caaacgcatg ataatggagc catccttgac ctcctcccca
    12001 atcataaagg acgaatcgga tctagtcaga agattggtca caaaactcat gatgatgtag
    12061 agggtgatat ccgccatact gaagccctga atcaaagact cttgcgaaga atcaaagaca
    12121 gccttccaga gataaaaggc cacaaaagcc cccatgacat cgccaatccg atagagaata
    12181 aagttgactc ggtaagtaat caactcctga acccctgcat tgataaaggg tttataacgt
    12241 ctccacaatt tgatcatcct agagctcctt tcggtagaag cgacggataa tatcctcaat
    12301 atccgtatcc accatcttca aatcgcggat ttcaaaatca gacagggttt gcttgataat
    12361 gtcagctgac tggtagcgag aactatcaaa ttcaatgttg aggctgtttc cttgtctatc
    12421 aatggtcata tcagacagac cttcatagtg agagacgaga tgactttgac ctggtagcag
    12481 ttcaaaagag agagtcttca tcttaccaaa ggtctccttg agttggctca ccgttccatc
    12541 aaaaatctct tgccccttgt caatcatgaa aatccgatca caaagttgct caatatcact
    12601 caaatcgtga gtggtcaaaa gaatggtagt ttcttcctct tgattaatct gagtaattgc
    12661 ccgacgaata ttatccttaa ccgaaacgtc caaaccaatg gtcggctcat ctaaaaaaag
    12721 aaccttggga ttgtggagca aggaggccgc aatatccgcc cgcatccgtt gtcccagtga
    12781 aagagtccgc acgggatcct tgataaagtc cttcaaatcc aagacttcat tcaaaaagtc
    12841 catgcgctta tgaaagagcg agtttggcac atcataaatc tcttttaaga cagtgtaggt
    12901 ctcttgcaga gccaaatccc accatagctg ggtgcgttgt ccaaagacta cgccaatatc
    12961 tttgacataa tcttgccgat tgtcctgggg aatcttgccg ttaatccgac aaaaaccaga
    13021 tgttggtttc aaaattcctg tcagcatttt aatggttgtc gacttcccag caccatttgc
    13081 cccgataaat cctaaaatct gcccttttgg aacttcaaag gtcaaatcct tgaccgcttc
    13141 aaaggtctgc ttttcaggat gaataaagga gcgcaaagcc cccttcaagc ccggttcctt
    13201 aacagtcttc acaaaatttt tctgaagatg ttccacttct atcattgcca tatctatctc
    13261 cctatcgtaa ggaatagcaa cattgtgttt ttaactacaa atattctaaa tccttacttt
    13321 ctacaccttc tacattttat tactacaaaa ggctttatat caacctatta tagtccaata
    13381 aaaaacgaaa tgcaagcgtt tgcctatgga ttgtgaaatt aggatttctc tcataaaata
    13441 atacttttaa tcaaaaattc tgtttaatag tttaaattac caacctacca cccttcccta
    13501 ctaaaacagc ccttgacttt ctctatcttt tttgtaatat atatgtaata caatataatt
    13561 acaaggagaa atgccatgtc aacacttaca cgagaccgag tttataactt ctgcgtcaat
    13621 actcaactat ttgaagaagc aaaaaccatc ctagaaagca aaaacatcag cctttcagat
    13681 gccctcaacc tctttgtaga acaaatcgtc cttgaaaaag gtctaacaat taaaacagcc
    13741 gaccaaccca aagcagaact agataaaggc taccaagata tcatcgaagg gcgtctctac
    13801 gacgcagacg aggtcttttc aaagtatgga ctataaagta cgcttcacag aacaagccag
    13861 acaagactta gacgatatct tcatctacta ctccacagag ttcagtgaaa acatagccaa
    13921 gaaagtcatc actcgtctac aacaaattac caacctactc atcttctcac cagagggcgg
    13981 tattagcttt gatcataaaa tcggatactc cctctatcca gaaaatatgc tcagaatgat
    14041 tgtctctaaa caatacctgc tcttttacct cattcatgaa aaagaagtcc ataccctgag
    14101 aattatcaac tcacgcaccg actacctaaa ccaactcgac cacctctttc gtacatgtag
    14161 gaaactattt tcctcacaaa taatctcatt ataaaatcca ataaattttt ttagctcact
    14221 tgaatatttt ctttgtatcg ttttatcagc ttcaatctga tctagacttg ttaccttgga
    14281 acgacttgaa ataacctttg taaaataaca cttaaggctt tcatcatctc gtataaattt
    14341 ttcaatagtg atataaaaag ctttcatatt ctgtttgtaa tcagatatta attgattaat
    14401 tttatcattc tgatgaagat tcgataccat aatatatagc ggattgaaat aagatgagaa
    14461 caaatcgatt gggaaagtaa aattaatttc tataaatgtt ttagcaattg tttcgtacta
    14521 ttttagattc agtctactat acataaacta acctcctcat tattagcaag gcttctactg
    14581 attataacct aattcttaga ctacttcaag actaactgtc caataataat ctacagataa
    14641 atcatccact cttaatcaaa ttttattcaa tccatctact cattcccaaa aggttgctgt
    14701 ataatgcaag acatttttct tactgatttt acttttcctt accctcaatt tagtgagcta
    14761 ataaattatt cttcttcaaa aggttgagat ttatctagta actctaaaac attaatggta
    14821 ggtagcaaca ttgcttcact gctatcaatt gccgaaacta ttgaaacaat agaacgtaaa
    14881 taaggataaa gaatagcagt accatttacg tatagaatat cggtgatatt ttcatctata
    14941 tttatttcaa aatatcctat tacagttaca gatatttttt tcatggcatc tttgtcaact
    15001 aaagaaacac taagttttac ggctccatga ctataatcat ctgtatgacc aaccttaact
    15061 atgggatata atatatcgaa ggtaaatgtg aacaaactga ttagggaatt taaaccaact
    15121 tctaacaatg tgttatactc aatgaaaacc aaagagcaga ctaggaaact agccacaggt
    15181 tgctcaaaac actgttttga ggttgtggat agaactgacg tagtcagtaa ccatacgcac
    15241 ggcaaggcga cgttgacgtg gtttgaagag attttcaaag agtattagaa agctcgatgg
    15301 actattccag attcattcca ctagatttcg aaatcgagta ggggataatc tctagcccct
    15361 ctcacaccac cgtacgtgcc gttcggcata cggcggttca actaactttt aacgcatgtc
    15421 gttcaaggta ataatccaaa cacgaaacca gtccacgttt ttcaaggact ggttttgata
    15481 tagcacgttt aagtaccgac ttctgagcta ctatagtaga ttgaaactag aatagtacac
    15541 ctctacttct aaaacattgt tagaaatcga tttgactgtc ctgaacgatt cgtcctattc
    15601 ttatttcatt ttattataat tgatagtggt cgccccagcc agatacctta tccgctatat
    15661 agaccttatt atcacgttcc tgttcgtaga gccacgattt cgctatccct tcctctgacg
    15721 attcttattt ccagcgtcag acttgggaat cgctattggg ttcaccggta gtaggtgccc
    15781 accgaggact tacacctcag atatacgaca tgcccgtcgt acatcaaaaa aagttgagac
    15841 atagtgtctt caactttttt acattagttt aactactgat taaagtgagt ttacgtcaac
    15901 tttgatacca acaccttgag tagttgtgat agtcaagttt gttacgtaag ttcctttagc
    15961 tgtagctggt tttgcttttt ggattgtttc gttgaaagct ttaaagtttt caaccaattt
    16021 ttcagcttca aatgatactt taccgatgat tgcttgaacg ttacctgcac ggtcagcacg
    16081 gtaagtgatt ttaccacctt tagactcttc aactgcttta gcaacatcca ttgttacagt
    16141 accagtttta gggtttggca tcaagttacg tggtccaagg acacgtccaa gacgtccaac
    16201 aagagccatc atatcaggtg tagcgataac tacgtcgaag tccaaccaac cgtcgttgat
    16261 tttagcaaca aggtcatctt caccaacaaa gtctgcacca gcagcttttg cttcttcagc
    16321 ttttgcacca cgtgcgaaaa caagaacacg tgaagtttta ccagtaccgt ttggcaatac
    16381 cattgctcca cggatttgtt ggtcagcttt tttaacgtcg atgttcaagt tgtaagcaac
    16441 ttctacagtt gcatcaaatt ttgcaaagtt agtttctttt gcaagtgcta cagcttcttc
    16501 tacactgtat gcttttgtgc tgtcgatttt ctcaagagca gcacgaagtt gtttgctttt
    16561 tttagccatt ttctattctc cttgtaagtg gttcaatcga ttttcatctc ccacgtcact
    16621 tctcgaaatg atgaagtctt gcgggttttc agtctactat ttttctcttc ctacggcctt
    16681 aagctccctt gctgtaccca agtacaagcc tcagtcactt gcctagtatg aaaagcaact
    16741 aatagactgc tgtgagattt tctactgtgt tattgattag tcaacaacag tgaatcccat
    16801 agaacgagca gtaccttcga tcatacgcat tgcagactct acgtttgctg cgttcaaatc
    16861 tggcatctta gtttctgcaa tttcttgtac ttgtgcacga gtaactgtag caactttagt
    16921 tttattaggt gtacctgatc ctttttcaac acctgcagct tttttcaaaa gaacagcagc
    16981 tggtggtgtt ttcgtaacga aagtaaatga tttatcttcg taaactgaga taacaactgg
    17041 aatgatcata ccagcttggt cagctgtacg agcgttgaac tcttttgtga atcccatgat
    17101 gttgatacca gcttgaccaa gagcaggtcc aaccggtgga gctggtgtag ctttaccagc
    17161 agggatttgc aattttacaa gtttttcgac ttttttagcc atttttaaat cctcctttgt
    17221 ggttttggcg gtaattagag atttttacct cccacaagta tgtttttcac atacccatcc
    17281 attatacact atttatctgg aattgcaaga gaaaagttta ttttttgtac tcaatgaaaa
    17341 tcaaagagca aactaggaag ctagccgcag gctgctcaaa gtacagcttt gaggttgcag
    17401 ataaaactga cgaagtcagc tcaaaacact gttttgaggt tgtgaataga accgacgcag
    17461 tcagtaggta aggcgacgct gacgtggttt gaagagattt tcgaagagta ttaatcgacg
    17521 tagtctccgc cttcaaagcc atagtattct tccaaactga gaccacttgc agcaatttct
    17581 ttagcttctt ttcctacata acgcaggtgc cattcttcag ccatatagcc tgtttccttt
    17641 tccttgcctt tgagataacg gacaacaaag ccataatcag ctgcatgatc caagagccat
    17701 tgggctgctt tttcttctgt caccaaatca ccatcagtcc caatcacatc aaaggccaag
    17761 cctgtctggt gttcgctata gccaggacgg gcagagtaac ggtcagctgc tgcctttcca
    17821 tcttggttga cataatcttg atagagcttg gtctgagttt cataacttct aaaaccactg
    17881 taatgatcac taatagggaa acctgcctct tgcatcgctt tgatgagttt gaccaactct
    17941 gccttggctg ttggattttc ccctggatta tagtctttag acaatggata gtgtttgttg
    18001 gctacgatga tttcatcgta tttcccttga atgctgtagt aatcaccttt attgaccact
    18061 tctgctttct tctgggcagc tccttgagac ttactaccga ctgttccatc agctttggct
    18121 gtctgttctg tcttagtttc tccatcttca ttttttgttt tttcttgtga acaagctgct
    18181 agactcaagg ctagcaaagc tgtcaagact aggtatcttt tcttcatttc tactccttta
    18241 tttctaatag tttatctact tctgacgata aacgtttgac taatctttta atctcatccg
    18301 gttttgcttg gcaggtttct gctgtcattt cttcaaaggg aacccaccaa actggtccac
    18361 gacctttaag accgtggtca ttcatatcac ctgtccgtcg tggaaacaga tgccaatgaa
    18421 gatgggcatc gccatttcct agcagttcga tattcatttt ctcagcagca aatgccttgg
    18481 caactgcctc ttggactaga ctcatttctt cgagaaaacg gagtcttgtt tcctttttca
    18541 aatggtgcaa ttcggtaacg tgttccttag ctagaaaaag actatatcct gcaaaatact
    18601 ggtggtctcc aatcacaaga tagcctgttt ccaactcttt gacaaagtaa ggattttctt
    18661 ccttcttgat gaggtcaatt ctctggcaaa tcaggcacat attagtctac caacccactc
    18721 ttaagatagg catcgaccat tcgttctgtt gcgaccaccg agtcaatatg agtgcgctca
    18781 taagaatgac tagactcgat acccgcacca agaagggcat gcttgacctc tgcccctgca
    18841 gacatagccg ctgaagcatc cgaaccataa aatggataga tgtccagctt aaaaggaata
    18901 tcttgctctt tagccaaggc aaccaagtgt tgacggaagt catagtgata aggacctgaa
    18961 gcatccttga cacagataga tactgtgtac tcatctgtct gctggtcatc tcccatagct
    19021 cccatatcca cagccagata ttctactacc tgagcaggaa tgttagaatt agcaccatgt
    19081 cctacttctt caaaaactga aaaagcaaaa tgagttgtta ctggcaattc aatcttctct
    19141 ttcttataaa tacgaagcag gttgagcaaa atcgctgcgc tgaccttatc atccaaatga
    19201 cgagacttga taaaacctgt atcagtcaca acagttcgtg gatcgaagct gataaaatca
    19261 ccgacctcaa tcccaagagc acgagtttct ttttcattgc ttacttttac atccaaacgc
    19321 acttccatat tgtcctgcgt gcgttctgca gttcctgcat ccttgtagac atggcaagaa
    19381 gtttggtgga tgaggatggt tcctgatacc ttttgacctg tgctagccac atgaacggta
    19441 cagttttcac cttcaatcat gttccaagga aaaccaccga tacggtccaa tttaagacga
    19501 ccatctggtt tgacagcacg aacaatagca cccagcgtgt ccacatgggc agtcacatag
    19561 cgatgttgct catcattttg acctttgata gtcacattga caccaccctt ggctgtgcga
    19621 actggctggt aaccaaaacc ttctagagtc ttgactaaat aattcgaaat ttcacgagtg
    19681 aagcccgttg gcgatgcgat ggctgtcagt tctttgatat attctactgt ttgattcatt
    19741 tcttcacctc tttcgctacc tattataaca catttatcat cggataaaaa aagaaaatca
    19801 ctcctgtaga cttggctaca aaagtgattt tatactcaat gaaaatcaaa gagcaaacta
    19861 ggaaactagc cgcaggctgt acttgagtac gacaaggcga cgttgacgcg gtttgaattt
    19921 gattttcgaa gagtattagt gattattcca tttcaaaaac atcgctttct tgcttgtttg
    19981 gtagaaccaa tgagagcaag attccaacaa tggctggcac caaccatggg agagatgcct
    20041 tggcaaaagg aagaacgcta acaagatttg caagaaactc aaccttaaac gagcttccta
    20101 gtacgcttgc aatggcaata actgtaacca cagcaattgt caactgcata cctggttttg
    20161 aaagagcaac aaatttgttg acaatgacaa tcataacgat agcaatcgtg attgggtaca
    20221 agataaccag tactggaatt gagtacttga taatcgcatc aagacccaaa ttggcaatag
    20281 caaatccaat caaggtaaag gctgtcgcat aaaccttgta gctgatttgt gggaagcgct
    20341 cattaaagaa ctcagctgtt gacacaatca aaccaacagt cgttgtgaag caggttacgg
    20401 taaccatagc tgcaaggaag agttgagctg ttgagccaaa gatttcttga gtggcttgtg
    20461 acaagatgta aacacctggt gttccaccct tcatcgcttc agctggtact gggaaatgat
    20521 ttccaagaaa acctaaaccg atgtaaagag cgctgaaggc aagggcaaca acgataccaa
    20581 caacccaaat agttgaaatg tattctttct tacttgaaaa tccaagttgt ttcaaggttt
    20641 gaactgcgat tacgctaaag gccactgagg caagggcgtc caaggtattg taaccttcta
    20701 ggaaacctgt accaaaggca gaagcttgat aagcagctga agcagcttga ggacttgttc
    20761 caccatattt gatagctccc agaacgacca agataacaat caaaattgca aagactggcg
    20821 ttaaaatacg tccaatgcgg tctaagattt ttgatggatt aagcgaaatc aaataggctg
    20881 ccgcaaaata cagaaccgta aatacaatca agccaagtcc tttatttgca tccgacaaaa
    20941 gggggctaat ccctacttcg taagctgttg tagcagtacg tggggtagca aagaatggac
    21001 cgattgacaa gtaaagaact gagaggtaaa gagtcgcaaa ccaaggcgct atcttcgttg
    21061 aaatctcgta gatataccct ttaggattta gcgttccaat aataagggtc aagacggcga
    21121 taccaacgcc tgaaaagaca aaacctgcga tggcaggaag aaaatgttct ccagatagag
    21181 cacctagaga aggcggaaaa atcaagttcc ccgcaccaaa aaatattcca aacaggagca
    21241 aacctgttag ggtacctttt ttagccataa aaatctcctt catatttata aaatactgac
    21301 ctagtatacc acgattagga gtctgaaaaa agcatcacga ggcaaatatt aaatgttttc
    21361 aattttctta aaaataagaa ttatctaaaa ataaaactct cctacccagt ccacaccaag
    21421 taggagaaat gcaaaatttt aaagttcttc aaaggctaac atgccaccct ggacattgat
    21481 aacattataa ccttgttcta acaggaattg gcaagcacgc gccgatctca ttccagattt
    21541 gcaaataata taatgcaagc gatctttgtc caattaatca taactatcag tcaattgact
    21601 aagcggtagg ttgtgggcac cttctaaatg aagagctgca aactcatcca cttctctcac
    21661 atccactaga gaaagctggt cgttttggta aagctggtaa aatgcgtcaa aggctatttc
    21721 tttcattctt tttcttctaa tactcttcga aaatctcttc aaaccacgtc agcgtcgcct
    21781 taccgtgcgt atgttaccga cttcgtcagt tttatctgca acctcaaagc tgtactttga
    21841 gcagcctacg actagcttcc tagtttgctc tttgattttc attgactata aaatggtttt
    21901 aattcttttt ttcaaatctg gcactacttc tgcctcaaac caaggatttt tggccatcca
    21961 gatttgattt cgtggtgatg ggtgaactag cggaaaatag gctggcagat agtctttata
    22021 gtgtttcacc ctctccgtta ccttcccact gattttctcc tgtaaatagt aggcttgggc
    22081 atattgccca atcaagaggg ttaactgaat atcaggcaat tcctgtaaga gctgcggatg
    22141 ccatttttct gcaaaacctg tacgaggcgg aagatcaccc gacttgccat gtcctggaaa
    22201 gtagaaatcc ataggcaaaa cagcaaaata acctgaattg taaaaggtat cttcatccac
    22261 acctagccag tcccgcaagc ggtcaccact tttatctttc cagtaaaggc ctgcttcttg
    22321 agttttaagt cccggagcct gaccgatgat attgatgcga gcagtttttg gcgctgcaaa
    22381 gagaggctca atgccacgct ctgtatagct ggcattctgt gaatccgcca tgatagcctg
    22441 tttgattctt tcgatttgag acatctactt tccctccaaa aagaagtcca agcataagat
    22501 ctcagacttc tatcgtttta tttgatcaat tcgtaaatag cttcggcata aattgctgct
    22561 gctcggaaaa gatcatccaa ggcgataaat tcattggctt ggtgcatggt atcaatcgag
    22621 tctgggaaca tagcaccgta ggcaactccg cgttctagca agcgaccaaa ggttccacca
    22681 ccgatgactt gttcatgacc tttaaggcca gtttgttttt catagatatt caacaaggtt
    22741 tgcacaagtg gatcttccat tggcacatag tgaggcgtgt gaccgtgttc agacaggcta
    22801 acagaaacaa ctggcaagtt ttcaaggatt gacttgattt gttctggact tgttcctttt
    22861 ggatagcgga tgttgagggc aatggtatta tcagcacttg tttcatcgaa gcggaagacg
    22921 ccggcattca tagaaagagc acccatcttt tcatccacat gagcaatctt aagattttca
    22981 ccctcatgat cgttcaagag aattttacct gcgatgtcaa ggtagtcttt ggctggacca
    23041 gcaaagccaa actggctgag gaagagggca aggtaagttg cgccattgac acctgaagca
    23101 ggcatagcac cgtgggctga tttaccaatg atcgtcacct tgtatttgcc agcttcttct
    23161 tggagttctc ctctaagttt gtgttctgca acaaaggcat ctagtttagc ttgcaagtca
    23221 gccaagtcac ctgaaacgac tgctgttgct gattctggta ccatattttc acgtaaacca
    23281 cctgtaaagc tgtgaagacg ggcaacacct gtattttctc ctgcaaagtg gaggtattcc
    23341 gtgatatttc ctttttcacc attgatgatt ggaaattcag catctggtga gaaaccgaaa
    23401 tctggtttgg caagtcctac gtgctcaaag tagtagtcca tgtctgccca gcctgattct
    23461 tcgtctgttc caacgatgaa gcgaactttc ttagaagttg gaagacccaa ttctttgatg
    23521 attttcaaac catagtaaca agctgttgta ggacccttat cgtccgaagc cccgcgcgca
    23581 taaaggcgac catctttgat agttggtgtg taagggtctg tgtcccaacc gctaccagca
    23641 ggcaccacat ccatatgggc aaagattccg agaacttctt ctccatcacc aaactcaaaa
    23701 tgtcctgcat agttatcaac attcttagtt gggtagccat cgcggtctgc gatttcaagg
    23761 aatttctcca aggcttttac tggaccaggc ccaaatggat gctgggcatc agccttgctg
    23821 tcatcacgtt ctgaattgat ttccaaaagg ctaaacaagt cagccaagag gtcttctttg
    23881 cgtttttcta cttctgctgt aaaatcaatt gctgtcattt tttcttcttt ctatcttttc
    23941 tcgatgattt catctactgg caagcggtag cttggttcca atttttcgtc tgtataaccc
    24001 actgtgatca agagttctgg gcggaaacgg tcttcgattt ccaaaacttc attaactttt
    24061 gatttgtcaa aaccaagaat aatgttagaa ccaattcctt ggtctgtcaa tgcaagaacc
    24121 aagttcatgg caaccaaacc tgcattgaga gctaggtagt cgctgacttg ttgctcactg
    24181 taacgggcaa actcagctgg cagatttttc ataaaatatt gaagttgctc ttcagaaaag
    24241 ttattagcac caccaacacg ggcaatctta cgagcacgtt tggctaagtc cgtatctgta
    24301 aacaaggcaa tggttacagg cgctgatgat acctgttcaa aattggaacc ataagctaac
    24361 tttgccagtt cagcattttt ctcacgtacc accacaaatt tccaaggctg gctgttgtgg
    24421 gcgcttggcg ccaaggttgc aatttcgata gccgtacgca catctttggg atcaacaggc
    24481 ttatcagtaa aatgcttagt cgcatgacgt tttttattta attcaagaaa tttcataatc
    24541 gatttccttt tctaattcta ctgcttccat tctaccataa tttgaaagag cttgcaggga
    24601 tttttcatga ttaagttttt ctatcacaga cataaaagat atatattggt ttatcaggaa
    24661 agtttctgat aaactagcaa gtactttaca tttgaatgga gacagtatga aaaaatttag
    24721 cctattacta gctatcctac catttttggt tgcctgtggg aatcaagcta cacccaaaga
    24781 gactagcgct caaaagacaa tcgtccttgc tacagctggc gacgtgccac catttgacta
    24841 cgaagacaag ggcaatctga caggcttcga tatcgaagtt ttaaaggcag tagatgaaaa
    24901 actcagcgac tacgagattc aattccaaag aaccgcctgg gagagcatct tcccaggact
    24961 tgattctggt cactatcagg ctgcggccaa taacttgagt tacacaaaag agcgtgctga
    25021 aaaatacctt tactcgcttc caatttccaa caatcccctc gtccttgtca gcaacaagaa
    25081 aaatcctttg acttctcttg accagatcgc tggtaaaaca acacaagagg ataccggaac
    25141 ttctaacgct caattcatca ataactggaa tcagaaacac actgataatc ccgctacaat
    25201 taatttttct ggtgaggata ttggtaaacg aatcctagac cttgctaacg gagagtttga
    25261 tttcctagtt tttgacaagg tatccgttca aaagattatc aaggaccgtg gtttagacct
    25321 ctcagtcgtt gatttacctt ctgcagatag ccccagcaac tatatcgttt tctcaagcga
    25381 ccaaaaagag tttaaagagc aatttgataa agcgctcaaa gaactctatc aagacggaac
    25441 ccttgaaaaa ctcagcaata cctatctagg tggttcttac ctcccagatc aatctcagtt
    25501 acaataacac tcttcgaaaa tctcttcaaa ccacgtcagc tttatctgca acctcaaagc
    25561 tgtgctttga gcaacctacg gctagcttcc tagtttgctc tttgattttc attgagtata
    25621 agatatacta aaagcgattg gaccagccaa tcgctttttt agtttgcatt ggctatttta
    25681 ggaaactctt acaaaaaatc aaaaaaatcc ttcacatcct ctacataccc atgcttttct
    25741 attaagctgg ctaataattc cagattgtgc ccctgatagt tatcttcacg acgcagttct
    25801 tcatacattt cgataaaggg aagccccttg gacttggcca attctaactc agcttcgtaa
    25861 tcataagcaa ctttacgaaa ttggatattg agtaattctc catcttcaac ttctatcacg
    25921 gcatactggg cacggtgatt ttttaacgcc tcccaattaa aatagggcat gccaatcgac
    25981 cctggattga tgatttgttg cccttgactt ccataacgaa gcaactgctt gtgaacatga
    26041 ccataaactg ccacgtccgt ttccgcatct agtagttggt caaatttctc tgtatcattc
    26101 tcaactagca agtcaccacc atagtttttg tcaggtaaat tatgagagat agaaaagcgc
    26161 aatccgtcaa tttctttctt ttccagcaaa ggcaagcttc gtagccagac aatcgttgca
    26221 ggatccattc gctccatcaa aaactgggtc atacgcaaga gctgaacttc ctgtgggtct
    26281 tctaagccat attgcccatc taaagcctca aggacacgat catcccaatt gcctcgaaca
    26341 cttgctgtga taggaaggtc ctttagcagg gcgactaagt catttgcgcc tggaccagga
    26401 agaaaaatat ctcccagaag ccaatattca ctggcccctt gatttttagc atctgcaatc
    26461 actgcttcta gcgccgtcgc attgccatga acatctgata aaattgcgat tttatggttc
    26521 attgtggttt ccttccgttt ggtaatctac tctttcttct gctacttgag gcaaggcttc
    26581 tccctgcggg ttcaactttc tttgcacagc ctctgcaacg actctaggta ccccaacttc
    26641 gacaatctca tccacactgg cttccttgat tttggtcaaa gacttgaaat gcttcataag
    26701 attctgcttg cgtttaggtc ccagaccgtc aatcccatcc aattgagatg agaaagaatt
    26761 tttggagcgc agttggcggt ggaaggtaat ggcaaagcgg tgcacctcat cttggatgcg
    26821 ttggaggagg aaaaattcct gagaattgcg agacaagtcc accacctcaa gcggatctcc
    26881 aaagagcaat tcatgggttt ggtgcttatc attcttttgc agcccagcaa ttggaatatc
    26941 caagcccagt tcctcttgga taacctgctt agcgatattg acttgacctt gccccccatc
    27001 aatcacaatc aaatctggag gagtcaaagc ctcacgctgt actcgaccat agcgtctgcg
    27061 aatgacctct ctcatgctgg catagtcgtc tggtccaaca accgttttta tcttgtactt
    27121 acggtaatcc ttcttactcg gtttaccgtt gacaaagacc accatagccg aaacagggct
    27181 agttcccatg atattagagt tatcgaagga ctcgatacgt actggggtcg ggatttggag
    27241 caaacgtcct agattttcaa tagctccttg ggtcttctcg acagatttct ctagcaggtt
    27301 aaatttctgc tccagactaa cacgtgcatt ttttatggct agattgacca gctgtttttt
    27361 ctctccacgt tggggtttga gaatcttggt atccaccaaa gccttgacag cctcttcgtc
    27421 aatatcctgc ggaatcagta cctcattggg aactaggtga gatttttctt gatagaattg
    27481 tcctacatag gtcaaaaaat cctcatctgg atcattgaag taggggaaga gattgacatc
    27541 gcgctcgatg agcttaccct gacggacaaa gaaaacctgc acacacatcc agcccttatc
    27601 cacatagtag ccaaagacat cgcgattttg gagatcctta gccatgaccc gttgcttggt
    27661 tcgaagcgtt ccaatagcct gaatcaggtc acggtattcc gccgcacgtt caaactccat
    27721 agtctgggct gccgctgcca ttttgccctt tagctcatca atgattttgt tatcctgccc
    27781 tttcagaaaa tcagaaacct cctgggccat agacttgaaa taagtctcat ccttcttaca
    27841 gatggtgtgg gccatgcatt gaccgatatg gtaataaaaa cagaccttag agggcgggtt
    27901 ggtacactta cgaaaaggga atatccgatc cagcaaccgc ttgatttcat tggctgcccc
    27961 cacatcggga tagggtccaa aataaagacc tccgtccttt ttgacctgac gagtgataat
    28021 caagcgtgga tagcgctcat tggtgatttt gatgaaagga taggacttgt catccttgag
    28081 catgatattg tacttgggct tgttttcctt gatcaggttg atttctagga gaagtgcctc
    28141 aatattagac tccgtaacaa taaattcaaa atccacaatt tcagacacca gagcctctgt
    28201 cttggtatca tgacttccac gaaaatagga ccgtactcga ttacgcagat ttttagcctt
    28261 tcctacatag ataatggtgc catttttatc cttatgaatg tagcaaccag ggctggtcgg
    28321 caagagctct agttttgatt taatcaagtt gttcatagtt ccattatagc aaaaaaagta
    28381 gggaaagata gtaccctact cactagtctc atatattttt cggaggtttg caacatcctc
    28441 ttcctggata ccataaaagg aacctgctgg ttgcatgaca gacttctcat ctgtatggtc
    28501 caagccaatc gcatgaccta actcatgttc tgccgtatgg acaaggcgtt cataggagta
    28561 gccatagtat ggattggaca aataataatg attcaaccgc accgttacgg acaagaattg
    28621 ccctgttaag agattagttt gactttccgc ctctcctgcc acaggagtgc ctccgtcgtt
    28681 catctccgta gccgtaatat ccgccttact agactcagtc acgagttcaa agttaaaagc
    28741 acccgtttga ttccagttct ggattgcttc taaataagcc tcttgaaagc gtgaatccat
    28801 ctgcggatcc aggtaaatac gagcagaggc ctgtgcccac cttccttcag aatgctggtg
    28861 actatctgtc tggcttaact taggcagttg ccctgttttc tcccattggt caatactttg
    28921 ttgaccaatt tttactgacc gttctgcttg ctttagcgct ccttgaaaat ccccgttcag
    28981 ataccagaga agtccgaaag caagcacaca taagagcaca actatccaaa ccagacgcca
    29041 aaacaaacgc cacacaaaag aaaagaaagc ccctatcaaa cgaaaaagcc agcgcatgtc
    29101 tctccacctt tctagcaaat aagaattatt ttactaaaag aagctaaaag aaatctaaat
    29161 actgactttt tcatcttaac gtcctactat ttttttgagt aactggatag ctttgtattt
    29221 taaaggcgat ggatggtaac ggaaagtacc cgctttgcgt acaatatagc cattaaaatt
    29281 ctgtttaaaa cgcaaaacac catcacttcc atcaaaaatc ccttgaatgc ctaggaagtt
    29341 gtatttaggt attccacgtt ttatgctttc caacataaca tatttttgaa gcagtgcagg
    29401 ggcatagaac ttattaaact cagtgtagga accactaaag agataagtcg tttcctgagg
    29461 catataaaca aataaactac cagctaaaac aatatcttct tctccatatt tttcaatcaa
    29521 gtctcgcgct tctgcttttc gaacttcaaa cgtttcaaat tgactagaat attctctcag
    29581 ttgattttgt tttttctcag aatgaggatt tttactcaaa tcaagtcgca acttgtccaa
    29641 tttttcttct agtttacttt gttcaccttg caatttgctc atatactccg aaaaattcaa
    29701 gcttgctatg agaaactccg cttgttctcc aaaagtatca taaaaatgct cataatattc
    29761 taaactttta tcactatatt ctctacgttc agaggtttct tttgttatat tcttaaaaat
    29821 cgatagttct tcacgtttta actttttcaa ccgaatgcca aaggtttcag cctttttcac
    29881 caagggttta ccctttttgc taaaactttt aagcaaactc ttttcagtta attcagttaa
    29941 atctttatag tataaccaat ctggttctcc acctgggtaa cctgttgtta agccatcaaa
    30001 ttgataacct aaatcagtca aaccttgaat aatacttttt ttctcagcat ctattggatt
    30061 accttggcta tcaaaagttt gataagtttc ataaggtttt acaagcaact ctaatacacc
    30121 attttgcttg gcatattctt ttaactctgc ataaaaaact ggaagagcat cttgttgggt
    30181 ataaatcggc cccgaattga gttccatatg cagaccaccc agcatgggca agctataaac
    30241 cagagctgca acttgaattt ctccttcttg tttcaaagca agataaacaa ttcgagcccc
    30301 tcttttttct agcaaatccc ccatctggac agattgcata aaggaacgag aagaaacctg
    30361 atcagaataa gtctgaaact cttctttcgt gagtgttgtt agtgccatat acttactttc
    30421 tatgtttttt tcttaatgtt ttacggaaat caagagcaag tcttaacaga ggatagagag
    30481 gatgagtggg cattgtaaat tcacccaagt attcttcaat cgttggatta aatttttcct
    30541 taaaatgata aagtccacca ttgagagagt tttcaacacc acctaaattt tgccagacca
    30601 tacctcgctc aaaggcatag cgagccgttt cataccatgt taaaattggt gcattgtaac
    30661 gtttaaaatc atcatccata ccagcatata tattgacaga ggtagtacca aattccaaac
    30721 tcaaagtagc cgctaaagga actctcgctt gacctacatc tatatattcc tgcaagaagg
    30781 tcaattcctc taacaaacgt tctttttcct tcttctgcgc ttctactttt gaagttcgag
    30841 tcgactcagt aaaggtctct tccaaggctc tatttttcgc taactgttct tctaattctt
    30901 gcgaacgttt agaaacatcc aaggtggcca aggtgatata ggccttgtcc ttaaaattat
    30961 ctaacaattt tttataatag gcttcattcc tcaaatgaat ctcttttcgc ttctcagttt
    31021 ttttcatcaa ctccgaaaat gaatctaata gttccagtcc accatattga atctcaagcc
    31081 ctttgtttcg tgctgttcga atagcctgtt ttgttgactt ggaaagttta tcttcttcaa
    31141 aattttcctt gtatattttc gcctgaatac gaggttgaat ggtgtctccc atttcctccg
    31201 tttttcctga ccaccttact cccatttgtt gcaaactatc aataatagcc agattttcag
    31261 gaaattctgt cttttcctga ttgattaaac tttgagatag gcaaatactt gggtcaaaag
    31321 tcacaaaaac cgctctctta ctgcgagcat aggacttaat agactgaatg gcaaaattca
    31381 agagttcttt atccccataa tccaatatag gtcctcttgg gatgtaaaac attttatagc
    31441 ctagcggaag agttctaatc aaaatactag ctgtcgccag taatttttct tccctgtaaa
    31501 caccaaactt ctcatgttgc caattagact taacttcctc ccaagcacta ctttgtaata
    31561 cattggctaa ttcatgttct ttgacaaact gatcatattc taatgtggga atgccaattt
    31621 gataacggta catttcctac tctctttcct ccagtatttt attactattc tactactttc
    31681 gtttgaaaag tctagtgaaa acatatttta ttttagttct actcccccta tgctgaagtt
    31741 caaactaagt cagtctctct tctaatttga aatcaattgg tagggttgtt aaaaaaactt
    31801 ccaattgttt ttcccagtag taccactcgt gagttccagc actatggcta taggtcacat
    31861 caaaacctag ttttttgaga tttttcactg cgagattatt ggcttcgtac aagaaatcct
    31921 gttcgccaca ccacgcccaa agtttggtct ttttatccga ttttttagcc agactttcaa
    31981 gagaataggg actagttgtc cagtctctaa tctctccaaa aacacctctc cagtaggctg
    32041 gacttcccag attttgactt tcaggagaaa agttttgaaa gctgagggca cctgaaaaac
    32101 tagctgcatg agaaaaacga tttgtcgtaa gagccagttt gaagcagccg tagcctccca
    32161 tagaaagacc agcgataaag gtcttttcac gcttgctagt catattaggg aagaagcgtt
    32221 tcagaacctg tggcaattcc tctgctagag ccatgtagta gtcaaaacca tactgggtat
    32281 cggtgtacca accattgctg gtattgggca taacaacgat gagattagtt cctcgaagca
    32341 agcgttctac attggtccgc ttaagccaac tattatgatt tccagacatc ccgtgcaaaa
    32401 ggtacaagac gggaatatct tcacactctg gttcttccac tcgattggca tcagggtaga
    32461 ggacattcac cccccactcc atatccaata cttgtgagta atactcgatt ttcattactg
    32521 ccatgatttt ctccttctat ttttctataa gaaaaagccg aaactcgact ttctctctgt
    32581 ttatttcaac gattattttg cttccatgac tactggtaaa atagctggac gacgcttggt
    32641 ttggtcaaag agatacttgg tcaaattatc tcgaaccttc cctttaagat ctgcccagtc
    32701 aaagtcatct ccttgaagat actcttctac cgtttggtta atcaattctg aactttcacg
    32761 gagaatatcg cgactcttct tgagataaac aaatccgcgc gtatgaacac gagccctagc
    32821 cacaattttc ttctcacgac ggttgactgt aatagccacg atgaaaattc catcctctga
    32881 caagacctta cggtcacgaa gaacaacatt tccaacatca ccaatggcat tcccatcaat
    32941 caagatatct cctgctgaaa ccgatccagc tggaacaaag tctccattct cgtaagccat
    33001 ggtcgtcccc tttttaggaa tgaagatgcg ttctggcaac atcccaactg ccatggcagc
    33061 cttagcgtga gcatccaact cacgatactc cccttggaca gggaagaggt acttaggttg
    33121 caaaagattg atcatcagct gcaaatcacg cacatttccg tgccctgata catgtaaact
    33181 ttgggtaatc aatttgacaa ccccacctgc ctgataaatc atattttcta cacgcgcaac
    33241 aaaggcttct ttagcaatag acggagccgt agcaatatag accaggtccc catccttgat
    33301 ttctacataa cgatggcgac caatcgacat cttacgaagt ccattgatag gttcacccat
    33361 acgacctgtc tcaagaataa tcaactcatg gtcttcaaag cgagacatat ctttaggctt
    33421 aatcaagaga atttcgttgg ctaaagacaa cttcttaaga cgaatcgctg tgcggacgat
    33481 attttcaata tcaaatcctg tcaagacgat acgtcgacct gttttatccg cagcgtcaaa
    33541 aatctgctga atacgagaaa gattactgga aacagctgca acgatgatac gaccttccca
    33601 gtcagcaata gtttgggtaa tttcatccct aacttcactt tcactagcca cctgaatatt
    33661 gctgtctgca ttggccgaat cactgaggag agccaggacg ccgtcacgac caatctctgc
    33721 caaacgagca aaatcagttg cataagattc actagccgtt tggtcaaatt tgaagtcacc
    33781 tgtataaacg atgcttcctt ccgatgtctt caagacaatt cccagactct ctggaacgga
    33841 gtaagtcgta gggaagaagg aaaccactgt cccaccaaaa tcaatctccg tattctcatc
    33901 aatgacatgg aaatcattaa atttcttaac ggcatcattt cctttgacaa agagctttgc
    33961 caactcaatg gtcaactcag acccaaatac aggaacttta gcctctgcca agagataagg
    34021 tagagcacca atggcatccg catgcccgtg ggtcaagaaa accccagcaa tacggtcgct
    34081 attttcaaaa aggtaatcca tgtttggaat caccacatcg acccctaatt gttcattttc
    34141 aggatatttt aaccctacat tcaaaacaaa aatggactct ccaatttcag caatgtacat
    34201 attttttcca ttctcacgca caccaccaag tgttgttaaa ctgatattac tcatttttcc
    34261 tccaaaatct tagtttatct tgattatagg gcaactctac cctcttattc taaaagttct
    34321 gatactttca gccgaatctt ttcctaccat tataccattt ttttgatgat tttcaaaatg
    34381 aaggtctgtt ttcaaaaaag agttgatttc ccaactcttt cctaacttct actctttttc
    34441 catcaaaaaa tcataaattt cttgcaccgt acccagcgcc atcatttctt ggtctttaac
    34501 ggtttgtttg agattttggt aaatttgact ttctcctatc tcccgctttg gcgcctcttt
    34561 attgactgtc atcacaccat cttttatcgt cacaaagcct agttcttcaa atacttgaat
    34621 catcttgacc agcaagattt gttgaatatt aagatatgca gccaaatctt tcagcttgta
    34681 gcgaatatca aactctggga actggtaaat agtcttgtac aatttggcaa actgatctct
    34741 agtcccataa cctgtcagat aataggcctt gtcaatatca tttttgaaat agacagcaga
    34801 gaaatgctgt tcctgaaaaa tggtcttcag ctgagtaata tcctctggaa tgttttttac
    34861 gacaacagct tcactagccg caagatttgg cagttctcca ggaaaatcca agactggaac
    34921 accttctggc aagactgcat tttttccacg aatgttaaaa agttgaacac cttccactcg
    34981 cgcatccatc atcatcaact ggagggcagt ttggccgttc cattggttga cagacaattt
    35041 aaccgctaac tctagattct tggtttgaga aaactctgtc gcccatctgc cttgaccaaa
    35101 ggctaccact tcaaaactca cctcaccctt ggaaattttc agctttagat gggcattacc
    35161 tgcccccata gtacgagcac tttcgacctg aaaattcttg atataaaaaa taggtttctg
    35221 attatccatt ccaaaaggag ctaaacgttc aaaacttttg accgtttcca agctaagtgc
    35281 ctccaaatcc aactcttcat ctaggtttaa cttattcttg ccaccagcat ctgcaccttt
    35341 ttcacgaaca taatcttcca aaatctgaga taaatctgag agttgctcaa cttccagcgt
    35401 catacccgct gcacctgcat gacctccaaa ggcgatgaag aggtctcgat agggatccag
    35461 agcttcaaaa atatcgaccg cttccacact acgagcactg cccttggcac gaccgtcttc
    35521 tatattaaga acaatgactg tctgtcccaa ttcttccaat aaacgaccag ccacgattcc
    35581 tagaacccca ggattccagc cttccttggc caagacctga accttcttct caggatccac
    35641 catggtcttg gcttcttcat agatagactg aacgatttcc ttgcgctctt cgtttttctg
    35701 gtgaatcata agggcaatct catgcgcttc ctcatcatca aatccagtca acaaatcaat
    35761 ggcaggattg ggatcatcca agcgacccaa ggcattcaaa cgaggagcaa tctggaaacc
    35821 aaccgtttct tctgttactt cgttagcagc aatcccagcc atgtccagca tttcttgcag
    35881 accaatgcgc tgggtatgac ccaacatttc cagaccatat tgaactaaga tacgattttc
    35941 atccgtcaga ctcaccatat ctgcaatagt tccaatagcg accaaatcaa gcaattccac
    36001 ttgcacttct tctaacaggg cacaagccaa cttgaaagca actccacaac cagccaaata
    36061 tttaaaagga taatccgcat ctggatgttc aggatggaca atagcataag catctggcag
    36121 ggtttcaggt atggaatgat ggtctgtcac aatgacatct actcccatag actgagccaa
    36181 tgcaatagcc tcatgaccag caaccccatt gtccaccgtc acaatcaagg aaattccttc
    36241 ttgctcgata aagtatttat aaacactagc attagggcca tagccatcgg taaaacgatt
    36301 tggcaggtaa actcggcact ccgcaccaag ttgttccaaa ctttccttca caatagaagc
    36361 cgaagtcatg ccatccgcat catagtctcc ataaacgaga atattttccc cttcttcaat
    36421 agcctgacga atccgctcca ctgccttgtc catatcatgg agcagataag catcatgtaa
    36481 gtcctccaag gaaggttcta aaaacttctt cagactttct tggtcctgaa tccctctctc
    36541 aaacaataac cgagccacct caggacccag tccagccttc ttggctatct ttgtaaaatc
    36601 cgcatcttct acctgcaggg caaactgcca ttcataagta ggtgttatca aaaagacatc
    36661 cactctttct aagaattcta ttgcttcatt ataacatgat ttagacgttt tctctatcca
    36721 gattgctttt ttataacatt ttatagtata ttgaaactag aatagtacac ctctacttct
    36781 aaaatattgt tagaaatcga tttgattgtc ccgatcgatt tttcctgttc ttatatcact
    36841 atagtgaatt ttttcagata tgatttgaca agaacattct tttatcgtag aatcatgtta
    36901 taaaatctaa cataaaggag ttctctcatg gctttagtag aatttaaaaa cgtcgaaaaa
    36961 tattacggag actaccacgc actccgcaac atcaatctcc gttttgaaaa aggacaagtt
    37021 gttgtcctgc ttggaccttc tggctctggg aagtccactc ttatccgtac gatcaatggt
    37081 ttagaggctg ttgacaaagg aagtctccta gtcaatgggc accaagttgc tggtgccagc
    37141 cagaaagatt tggtacctct tcgcaaggaa gtcggcatgg tttttcaaca ttttaacctt
    37201 tatccacaca aaacagtgtt agaaaacgtg acacttgcac ccattaaagt tctaggaatt
    37261 gataaaaaag aagctgaaaa aaccgccaaa aaatatctgg aatttgtaaa tatgtgggac
    37321 aagaaagatt cctatcccgc catgctatct ggtggacaaa aacagcggat cgccatcgct
    37381 cgtggtcttg ctatgcatcc ggaactcctc ctctttgatg aaccaacatc tgctcttgat
    37441 cctgagacta tcggagatgt tctagcagtt atgcagaaac tggcgcgtga tgggatgaac
    37501 atgatcatcg ttacccacga aatgggcttt gctcgagagg ttgcggaccg cattatcttt
    37561 atggccgacg gagaagtttt agtagatacg acagatgtcg ataacttttt tgacaatcca
    37621 agcgaacctc gtgcccaaca attcctcagc aaaattatca accacgaaag tgacaaagtc
    37681 aaataaggag gcgcctatga aaaaaaattc tttttatcag cattattgat tagccttttc
    37741 ggccttgctg ctgccaaacc agtccaggct gatactagta tcgcagacat tcaaaaaaga
    37801 ggcgaactgg ttgtcggtgt caaacaagac gttcccaatt ttggttacaa agatcccaag
    37861 accggtactt attctggtat cgaaaccgac ttggccaaga tggtagctga tgaactcaag
    37921 gtcaagattc gctatgtgcc ggttacagca caaacccgcg gcccccttct agacaatgaa
    37981 caggtcgata tggatatcgc gacctttacc atcacggacg aacgcaaaaa actctacaac
    38041 tttaccagtc cctactacac agacgcttct ggatttttgg tcaataaatc tgccaaaatc
    38101 aaaaagattg aggacctaaa cggcaaaacc atcggagtcg cccaaggctc catcacccaa
    38161 cgcctgatta ctgaactggg taaaaagaaa ggtctgaagt ttaaattcgt cgaacttggt
    38221 tcctacccag aattgattac ttccctgcac gctcatcgta tcgatgcctt ttccgttgac
    38281 cgctctattc tatctggcta cactagtaaa cggacagcac tactagatga tagtttcaag
    38341 ccatctgact acagtattgt taccaagaaa tcaaatacag agctcaacga ctatcttgat
    38401 aacttggtta ctaaatggag caaggatggt agtttgcaga aactttatga ccgttacaag
    38461 ctcaaaccat ctagtcatac cgcagattaa ggaggatacc ctatgacaga tttatcatct
    38521 tggacagcct attttcagga ttttggacaa tttttcaatg gtttcctctt tactcttgcc
    38581 ctagcgattg gatcctttat cctcgccatg gtcttaggaa tcttctttgg tgccatgtca
    38641 accagtaaac gtccaatttt acgcatttta gctcgcatct ttgttgaatt ttaccaaaat
    38701 actcccctct tggtgcagtt tgttattgtt ttttatggcc tacctcttat cagtgaccat
    38761 atcatcatga ttccaattta ttggacagcc gttctctgcg tgggactcta tcacggcgct
    38821 tatatcgctg aggttattcg ttcagggatt cagtctattc ctagtggtca gatggaggcc
    38881 gccttgtcgc aaggttttac ctatatcagt gccctgcgct tgattatctt gcctcaggcc
    38941 ttccgcatca ttctccttcc attgaccaac caaattgtta acctcatcaa gaacacctct
    39001 actgtagcta tcatctctgg agtagacttg atgtttgtga ctaagtcttg gtcggctctc
    39061 aacggaaact atatcccagg ctttttaggt gctgctcttc tctactttgc cctatgcttc
    39121 cctgttgccc agtttggtcg caagatggag caagccaaca aaaaagccta ttcactttag
    39181 gaggttacta tggaatctat tttagaagtt ttaaccccag ataacctagt ctttatcttt
    39241 aaaggatttg gcttgaccct ctatatttct ctaattgcca tcatcctctc tactatcatc
    39301 ggtaccgtgc ttgccgtcat gcgaaatgga aaaaatccta tcttacgcat tatttccagt
    39361 atttatatcg agtttgtgcg caacgttccc aaccttctct ggatttttac tatctttttg
    39421 gtgttcaaaa tgaagtccac accagcaggt attacagcct ttactctctt tacatcagca
    39481 gccttggctg agattattcg aggcggtctc aatgccgtag acaagggaca atacgaagca
    39541 ggaatgtcac aaggcttcac ctcagcccaa atcctctact acatcattct cccacaagcc
    39601 atccgcaaaa tgctaccagc catcatttct cagtttgtta ccgtgattaa ggataccagt
    39661 ctcctctact ctgttatcgc cctacaagaa ctctttggag ccagccaaat tctcatgggc
    39721 cgttatttcg aaccagagca ggtcttcagt ctttacatcc tgattgccct catctacttc
    39781 agctttaacc tagcaatttc tagcctgtct catatgctag ccaaacgttg gcaacaagct
    39841 gcagaataat actcttcgaa aatctcttca aaccacgtca gcttcacctt gccgtatata
    39901 tgttactgac ttcgtcagtt ctatccacaa cctcaaaacg gtgttttgag cagcctgcgg
    39961 ctagcttcct agtttgctct ttgattttca ttgagtataa acagccactc tccagttcaa
    40021 ttggagagtt ttttgatgat agtagatatt tatagacctc aatttgacac catacatttt
    40081 ctatgataaa atgtaacaaa tttatttaca acaaatcgaa atctaaatga aatggaattt
    40141 tctatgaaat cagtcaaagg actactcttt atcatagcta gttttatctt gactcttttg
    40201 acttggatga acacttctcc ccaattcatg attccaggac tagctttaac aagcctatct
    40261 ctgactttta tcctagccac tcgtctccca ctactagaaa gctggtttca cggtttggag
    40321 aaggtctaca ccgtccacaa attcacagcc tttctctcaa tcatcctact aatctttcat
    40381 aactttagta tgggcggttt gtggggctct cgcttagctg ctcagtttgg taatcttgcc
    40441 atctatatct ttgccagcat catccttgtc gcctatttag gcaaatacat ccaatacgaa
    40501 gcttggcgat ggattcaccg cctggtttac ctagcctata ttttaggact ctttcacatc
    40561 tacatgataa tgggcaatcg tctccttaca tttaatcttc taagttttct tgttggtagc
    40621 tatgcccttt taggcttact agctggtttt tatatcattt ttctatatca aaagatttcc
    40681 ttcccctatc tagggaaaat tacccatctc aaacgcttaa atcacgatac tagagaaatt
    40741 caaatccatc ttagcagacc tttcaactat caatcaggac aatttgcctt tctaaagatt
    40801 ttccaagaag gctttgaaag tgctccgcat cccttttcta tctcaggagg tcatggtcaa
    40861 actctttact ttactgttaa aaattcaggc gaccatacca ataatatcta tgataatctt
    40921 caagccggca acaaagtaac cctagacaga gcttacggac acatgatcat agaagaagga
    40981 cgagaaaatc aggtttggat tgctggaggt attgggatca cccccttcat ctcttacatc
    41041 cgtgaacatc ctattttaga taaacaggtt cacttctact atagcttccg tggagatgaa
    41101 aatgcagtct acctagattt actccgtaac tatgctcaga aaaatcctaa ttttgaactc
    41161 catctaatcg acagtacgaa agacggctat cttaattttg aacaaaaaga agtgcccgaa
    41221 catgcaaccg tctatatgtg tggtcctatt tctatgatga aggcacttgc caaacagatt
    41281 aagaaacaaa atccaaaaac agagcttatt tacgaaggat tcaagttcaa ataagtaatc
    41341 atactctcct ggaatttccc aggagagttt tttatagcca aatgaaataa gacaggaacg
    41401 atggaatttt ggcattcaaa tcaattgata agaatgtttt atagtggagt gaaataggat
    41461 acgaacaaat tgattaggaa aatcaaatga atttatagaa atcttttagc agttatgttg
    41521 tcctattcta gtttcaaaac gctatagaag cagcattgtg ctagtctaga ttcagtttac
    41581 tatactaaaa cgagtagctt gaaatcaaaa aacccaccct cacaggcagg ttttatctgt
    41641 attattcagc tagattatgc tttaccttct gaaccgaata cgtcgatacg ttcttcaacc
    41701 gatgcttgga tagcttttac accgtcagcc aagaatttac gtgggtcgaa gagttttttc
    41761 ttgtcgtatt ctgcttcgtt tgcttcgtag tcacgagcaa atttacgagt tgcgttagcg
    41821 aatgcgattt ggcattctgt gttaacgtta actttggcaa caccaagttt gatagctgct
    41881 tggatttgct catcaggaat acctgatcca ccgtgcaata cgattgggaa tcctggaaga
    41941 gcttctgtca atttttgcaa gtggtcaagg tcaagacctt cccagtttac tgggtaaggg
    42001 ccgtggatgt taccgatacc agctgccaag aagtcaatac cagtttcaac cattgcttta
    42061 gcgtcttcga ttggagccaa ttcaccttta ccgatgattc cgtcttcttc accaccgata
    42121 gtaccaactt cagcttctac tgagatacct ttagcgtgtg ctttttcaac aacttcttta
    42181 gccaatttaa ggttttcttc aactggaagg tgtgaaccgt caaacatgat tgaagtataa
    42241 ccaacttcga tacactcaag tgcatcgtcg tagtgaccgt ggtcaaggtg gatagctact
    42301 ggtacagtga tacccattga ttcaacaagg ttagcgatca agttgcgagc aactttgtaa
    42361 ccacccatgt atttagcagc acccattgaa gtttggatca aaactggagc ttttttagct
    42421 tctgctgcgc gcaagatagc ttgagtccac tcaaggttgt ttgtgttaaa tccaccaact
    42481 gcataaccgt tgtcacgggc tgcttggaca aatttttctg ctgaaacgat tgccatttta
    42541 tcaggcctcc tgtatatttt tatgggtcat cccatttaca ttgttcattt tatcactttt
    42601 tgccaaaaaa atctagtttt tcccgcagtt tcgattgatt ttcttctaac tccatctatg
    42661 taaacccttt ctctccctag tcttgggcga cttttggaaa agctataaag aaggttaaac
    42721 gattctcctc catctcgaaa cgataagcta atttttcatg ttctaataga ctcttaacca
    42781 caaagagccc cataccagac cccttgacct tgcgactagc attgtcagaa aaagactggg
    42841 ctagtttttc ttgttcctct gagctacagc tattttcgat aaaaagttct ccttctcttt
    42901 ctccaattcg aactaagcca cctggaacag agtgcttaat ggcattgctg atgagattag
    42961 aaagaatcaa cttcataact gatgggttta gataagcctg ctgatgggtc aaactattgt
    43021 ctatctggag ctctctttcc ttagctagca aggcataatc tttgaccaga ttttgcgtca
    43081 tctggaggag gtcaattgtt tccctatcat ctcgcaattc ctgcacagaa gagagggaaa
    43141 gtatctgcag aacatggtga ttgagttcat ccacaatccc caaggcaact cccagatact
    43201 ggtctctatc cttataacga ccgatattct ctctcatatt ttcgattagg attttcaaac
    43261 tagccagcgg tgttttcaat tcatgagaag ctcctcgtag gaattcgacc ttcatcttct
    43321 ccagctggag aatggcttca ttcttttcat gcaagtccgc aataacagtc aagagatgct
    43381 ggtagaggct attgatttgt tccttgagat tacctatctc atccttagaa tccacgcgca
    43441 atcgcacttg ggaatccagg tccatcatcc gaagggtcac ccgcttgatt tccaagatcg
    43501 gtgcaacaat agtccgagcg tagatgtagg ccaccaagag ggaaatcaga aaggaggcca
    43561 gcaaggtata gggaagaaac tggagactga tttgctccgc ttccttttgt aaatccatgg
    43621 aagctagaaa ctggagaatc atagtagcac cgtcttgcgt tttcacctcg cgctcctcaa
    43681 taaagagaga ggttgtctgg cggtctgtgt ccagaggaag actgtccttg acttctaact
    43741 tgtcctcggt catctcacct ttgacggtcc ccttgatatc actagtctgg gaatacaagt
    43801 ctaacacttg ctcgatactc tgcctatctt tcccttctag ggactgggca atggctgttg
    43861 ccttttgacc aatggtttcc tgacgatgac tcagataagt cgaaggaaaa agaaaataaa
    43921 tagctaaatg aaggcagata accagaacac taaatatcga gaaggtatag ataaatatct
    43981 ttgcaaataa acctgttcgt ttcattttcg ctccaattta taaccaacat tgcgcacagt
    44041 gaggatacaa tccaagtcta gctttttccg caattccttg atataaacat caataacacg
    44101 gtcaaaggga acctcatctg tcgctttcca gacggcatcg ataatctgag atcgagtcaa
    44161 ggcccggcct tcatttttca ctagatagtc cagaatttcc aactctttgg cattgatagg
    44221 cacttcttga cctgcgatgc ttgcactgta gctttcaaag tccaccttgg tatccttgta
    44281 agaaaagatt cgtcctgtat cgtagtagcg cttgaaaatc gcgtccaccc tcacttttaa
    44341 aagggagagg gagaaaggtt tttccagata gccatctgcc aaagaggcaa aggcactcat
    44401 cttgtattcc tcatcctgaa aagctgtcaa catcaagaca ggaacctgac tggttttacg
    44461 aatctcagct aggacttcta agccgttgag cttgggcatc tggatatcca gtaaaaccag
    44521 ggccacctca tagctagaaa attgctccag agcttcctga ccgtccgctg cctcaatagt
    44581 ttcatagcca caatccgtca aataatcact gaccccctca cggatcatct cttcatcttc
    44641 tacaattaaa attttcatac tttaactgct ctctattttt tatttttctt agaataaata
    44701 cctactctat tttctattat agtctcttgc tggccttttg tctgtaagca actgaccact
    44761 agataaaacg ttgtgaaatc ctttctcata aattccataa ctttagtata ttatatttaa
    44821 gcactaaagt acaaagaaag caactgaaag caatgatttt caccactgct ttcggattta
    44881 ttttgaattg ttaaatagcc attcctatcc actattcttg aatagaaaca caagatgcaa
    44941 tctttattct agactcattt tttcaaattt attcaccatc cagcaagagc tcttttggtt
    45001 gttttctaag gagattgctt gaagcaagca ccataacgag aaccactaga accaaggcaa
    45061 gaacaaaaat gatgataaag tctgatgtct gaatggaaat gtctaagctc gacaaggtct
    45121 tgctaaagcc atctacttct gcaccaccac caaggttaga ggcttgagcc gccttactag
    45181 cctgtttggc aacacctgaa gtcacattgg caaggacagt gtttccaatt gcacgggcag
    45241 tgtaattagc taggaagtaa gcagaaacta gagcagggat agcaatcaag atagattcgg
    45301 tgatgaattg acccaagata cttgcctgct tgaggccgat agagaggaga attcccactt
    45361 ccttgcgacg ggcgttgatc caaaggctga gcaagagggc aaggaggaga actgagaagc
    45421 tcaagctacc ccagaagagg aggttggcca tcttgtacat accagagata gattgctcaa
    45481 gagctgggta gttagaggag ctcttgacga gtgtgtagct cttccagttg ataccactga
    45541 tgccattcaa ctctttcata acatcatcca agttcttgtc tgctgttaca aagaaggttg
    45601 cgtccccata aatggctgtg tcttctgtgt atccataaag ttttgcagca gtgtgaatgt
    45661 ctgtaatagc tgtgttttcg taaagttctt gtgagtaggt tactgctgac ttattatgac
    45721 catcaaagag tcccttgatt gtcacttcaa ctgtttcctt ggctcctttt tcattatctg
    45781 catcgtagat attagagtcc agtttaacct tatcccctac tttccagccg tgtttggctg
    45841 ccaagtcctt gtgcaagagg attttatcct tgtcgtcgtt ggttaagtgc tctccttcga
    45901 ctagtttata agaaccagag acaaacttgt cttctttaga ggagtcattg acacctgtaa
    45961 tcatcaagct acttccaaaa cgtttggcac gatcagcagt gagattcttc ttggtttctg
    46021 gcgtttcaat caggtcatat ccagtcaaat ctccgatagc gttgatacgt ttgacataag
    46081 actcaatggc cttgttttcg gtgatttttt tgatgtcttc acccttgata ttcccagcac
    46141 cgcgaggcgt tccttggttg acgcgacgat tgatttgcat ggagaagcta ttggtgatat
    46201 ttttaaaggt ctcctgagaa gccttggcag tagctccctt gattgacaag ccgaccaaac
    46261 tcaagctcgc catgaggaga ataatcagga agatgacaat cgatttgaaa aacttccttg
    46321 taacataggc aaatgcgttg tgtaacatag attccctttc tagattttgt tttaatcatt
    46381 ctattaaagt aagctcaaat tatttactag tattgcgcgt ttcagtcagt ttcttatcct
    46441 ttaattcaag tgtaatatct gacgcttgtg ccacttcttt actgtgagtt acgacaatca
    46501 cacatttacc tgttttctgg gcaagtgatt tgagtagttc gacaatatct ccagcagttt
    46561 taggatccag atttcctgtt ggctcatcag ctagaataac tggagcttct gagaccaaac
    46621 tgcgagcaat ggcaacacgt tgctgttgac cacctgataa ctggagaaca ttccgcttga
    46681 tctggctttc atccaaacca agctcaagaa gtgtatcctt gcttgccttt ttgttgacca
    46741 agcggatatt ttccagcgga gaaagataat ctatcaagtt ataattttga aagaccaggg
    46801 aaatatggtg catgcgatgg taagaatagc ccttcttacg aatatcctct ccttgaaaaa
    46861 ggatagaacc ttcaacagga ctatctagac cagcaagtag ggacaagagt gtggattttc
    46921 ctgctcctga ctccccaata atactgtaaa attttccagg ttcaaaatta taatcgatct
    46981 gatataggac tgcttcagca gtattcttat aacggtaggt aacatcttgt aattgtaata
    47041 aagtcatgat ttctccttct taactaatag atgataaaat ttctttcggt gattttctaa
    47101 ataagaatag gaaacaaagg gctacagata agcaactaag cagaactaga aaaacatagg
    47161 attctgcaaa agataagatg ctagttgata aactgcttgc tttggctagt gtatcttgta
    47221 agcttgcctg atctccactt gctagtagag tttggagtag gtaagttgtg attccgtttc
    47281 ctgcaacaaa tgctggaagc aaagctccaa gagataccaa aactacctct aaacagaatt
    47341 gtaggaagat cgagctcttg ccttttccaa gtgcaagtaa aatccccact tcatagaccc
    47401 gttctctcaa ccagagagac aaaaccagaa ttaaggctcc agctcctgct atcaacatcc
    47461 cataaaggaa gatggtcagg aaggtttgga aagttgcaac tgagtctttg atttgttcaa
    47521 aagccttgtt ttccttttcg acttggtagc cttgattttc caaggccaag ttttctacct
    47581 gcttcatgag tccgtccatt tccttaggat tttctacata gaagcgtgct gcactgactt
    47641 gagcttcact attgcccaaa agggtttggc tactttcata gtctgtaaag acttgatttt
    47701 cactgaagtc agaagacaag cctgtgaatt tctcttgttt tttaccagaa aagatgccga
    47761 taatctcaaa ctctactgtt tgtccttttc cagattcaga ctgaccagca tccaagccaa
    47821 tcttatcatg aagtgaaaga ccgttcttct tagccaattc ttcgtggata aggattttct
    47881 tggaatcccc tttttgaagg tgtcgccctt cttttagatt gaaagccgaa ctggtaaagg
    47941 ttacatcctt ggatgaatcc tcaagagccg ttaagctaac caagttattg tctgcagctg
    48001 ataaatcatc acgctccacg ctctgctcgc cagtcactgc ttccttgtct tttagttttg
    48061 cgaccgtctc aagttcagga gagacatttt ccagcccctt aatcttgctt acagatgcta
    48121 ggtctgacaa cttgaatgtc tgaccattct ctatcttctt aatagaaaaa gatgtattga
    48181 gtgatttata cagattgctt tctactgttt tgttggactt catcagagtc aaacaggctg
    48241 aaattccggc caataagacc aataaaatca gaaataaaat aaaacttctc agtcgctttc
    48301 tgctgacata agcccaagat ctttggattg gattcatttg tcacctccat atttgtaaga
    48361 ctattataaa acccaaatat gaaatgttta tgaaatacga aaaaaaatat cgagtagggg
    48421 ataatctcta gcccctctca caccaccgta cgtgccgttc ggcatacggc ggttcaacta
    48481 acttttaacg catgtcgttc aaggtaataa tccaaacacg aaaccagtcc acgtttttcc
    48541 aggactggtt ttgatatagc acgtttaagt accgacttct gagctactaa ttgatagtgg
    48601 tcgccccagc cagatacctt atctgctatc catttaggaa cccctaactt aagcaatccc
    48661 cataatcgtc tcgatttctt cttccattgc ttccagataa tcactcgtag gcgagtacgc
    48721 aagcgctcat ctatgctagt gactatactt ttcatattta taattcattc ctttcgtttc
    48781 actcaaggca caacacagaa tgaaaaagtg ttgtgatctt tattttgttt tataataata
    48841 gtgagaaagc ctatcactac tacaaatcac ggggaggtga ataagtgagt ggtacagcca
    48901 ctacctcgca tattttgtca catcatttaa cggtacataa taagttgtac catctgaata
    48961 agttgctaca atatcatttg catgctctcc ttcaccttta gcaaaggttg gagctcctgc
    49021 tggatgattt ttatttgcct ctttcaattt ttcaataatg gcattttttt ctgtatcttt
    49081 tatattatca ggatttttca ctaagatttt gtctggatat gtcggtttag cagaaacaat
    49141 ttttactgtt atttcttttt tattcgaagc acttgtccag tttccagcat tatctttagc
    49201 atttaatttt acagtaattc ctgaactagg aacttcagta gcaggttgat tatcaacatt
    49261 attcaacttt aatttcaaaa gagctgttgc atcagacgtt ttatcaatcg ttgtatataa
    49321 tgatgaattg ttattataac cagtttcttc atatttagct gtttgtgagc tacttgaaac
    49381 agaactgaaa ttatgcccac tacctccctg attatcttca atgcttacgt ctaaatgaac
    49441 ttccccacta ttatttggct tagcaacaac tgttatagta aaataacata aaatttgcat
    49501 aaatagatta gggaaatcaa agcaacttct aggaatgttt tagcagtcac agtgtacttt
    49561 cccagcatca atccactata actctgcaca taaaaatgga gaagatggcc atcctcttct
    49621 ccaaatatta acttctttac aaaccaacta tagttgacaa agaacctaaa atcaattgat
    49681 aacacaaggt caggtcggtc aactctttca actgaagccc tgtcaactct tcccatttat
    49741 caatcttgta ttggagagaa ttgcggtgca gatagagttg ctgggctgtt ttagtgagaa
    49801 cagcactatt ttcccaaaga gagagaatga tttcctgaat ctgatcttga tccaaaatca
    49861 tctggtgtag acattcttta attggcttca agtccacgag tctttctccc atactccaaa
    49921 gatagagctg agaaaaagta tgaacacctt ggtgaccctg acgccaccat gtcttgaaca
    49981 aatcccgctc agctttgatt aagtctgata gggcttgatg tcctgtctga gaccaaacat
    50041 gacccaacat gatagaaaga cgaagtccaa agtcatactc aaccgcttca atcgtatcac
    50101 ttaaaatatc tcttacagaa gtgtatttgt cttgttgaag cacgaaaaca taatcctgag
    50161 atccgacctg tagcactgtc tgacaattcg gaaaaagagt ccgcatcata tctagccaag
    50221 aagccagatt ttcctgctga aaataagaaa gatggcaata aaccaactga atctttttaa
    50281 aaacttgcgg tgcctgtccc ttgccttcaa ccagatagga ataccaaggg tttagcgaac
    50341 gagcctgctc ctgctgggtc aaaagggcaa ccaactgctt ttcacgctcg ctgagcccag
    50401 cttcctccag caaaatccac tgctgagaag ctaaagggag cgtgagatag ccctctttct
    50461 ctactggttg gtctgaaatc cgagcctcag gaaaccagtc ttgtagttct ttttccctca
    50521 tgttctagcc ctccactttt tggatgcacc atgaaaccaa actctcaaga cgttccagat
    50581 tctcagtcat atggagatag cccataaccg cttcaaatcc cgtggacata cgataagtca
    50641 cgacatctgc atttttagcc tttgtgtggc tattggtatt gcggccacgt ttgtagattt
    50701 cttcttcttt ttccgttagg acctgctcct ccaacatgag agcaatcagg cgagcctgag
    50761 ccttggctga cacatacttg gttgcttctt gatggagttt attgggtttg gtcatacctt
    50821 tgaggatgag gtgacggcga atatacatag aatacaccgc atccccctca aaggctagcg
    50881 caatcccgtt aatgagattg acatcaatca cgtgtccacc tcactccatc cttggtatca
    50941 aggagcttaa ttccttgagt aaccaattgg tcacggattt ggtctgctgt ctcaaagtct
    51001 cgattggcac gcgcctcttg gcgtttttga atcaagtctt caatctctgc atccaaaact
    51061 tcctcaacaa agacaattcc aaaaatttct aacatatctg caagagcttg cttgacactt
    51121 gcatcatagt tccctgagtt gatccatttg gccatttcaa agacaactgt gataccgttg
    51181 gcagcattaa aatcttcatc catagctgct acaaacttat ctttaaagtt ttgtaactct
    51241 tgggcatcca cgtttcctgt aaatggttgt tcgtaagtat tcttcagata cttgagattg
    51301 gtctcggcat cgcgaactgc cttttccgtg aagttgatag gcttacggta gtgctgggtc
    51361 gcaaagaaga aacgaagtac ttgcccatca agagttttaa gggcatcgtg taccgtaatg
    51421 aagttaccca aggacttaga cattttgaca ttgtcgatat tgacaaagcc attgtgcatc
    51481 cagtagttag caaaagcctt gcctgtttta gcttcagatt gggcaatttc attggtgtgg
    51541 tgtggaaact ctaggtcagc tccaccaccg tggatatcaa tggtatcacc taaaatctct
    51601 gtcgacatga ctgaacactc aatatgccaa cccggacgtc caggtcccca aggactatcc
    51661 caagaaatct cacctggttt ggaagatttc catagagcaa aatctacagg attttcctta
    51721 cgagccgttt cttcatcggt acgacctgaa gcacctagct ccaaatcttc caaggtttta
    51781 ttagccaatt tagcatagtt gtgggatttt tctacacgga aatagacatc cccttgactc
    51841 tcataggcaa agcctttctc gatcaagtct tccacaaaac ggatgatgtc tgccataaac
    51901 tccactacac gcggatggcg agtcgcaggt ttcacgccta aggccgtcac atcctcacga
    51961 aaggcagcga tgtacttatc cgcaacctcc tgaggtgtga tgccttcttc cctggcacgg
    52021 ttgataatct tatcatccac atctgtaaaa ttggaaatat aggcaacctt atacccacgg
    52081 tactcaaaat agcgacgaat cgtatcaaaa gctaccgtcg aacgggcgtt tcccacgtga
    52141 atatagttgt acacggttgg cccacaaaca tacatcttga tcttgccgtc ctcaatcggg
    52201 acaaattctc gcaaatcacg agacatggtg tcatagattt taatcataaa tcataatcag
    52261 gaaagctaaa atccaagaac tgttagtttc atcactaaac taagagctca agtaaatttc
    52321 agtccgaatg tctctacact tcggaatccc ttgctccttt ctcattcaga taaaccacct
    52381 gtgtctgttt gacaaagcca attttttcat acaaacgttt ggcacctaca ttgctatctt
    52441 ccactgcaat ctgaaattcc ttgtcatttt gctcaattag ttggttgacg agggattttg
    52501 ctaagtagct tccatagcct ttcccacgtt caggttccga tattgctaaa ccgtagaagt
    52561 aattcgtatt agtcgataaa tctaccgtgc aagtcccaat aacctgaccg tcttttaaca
    52621 aaatatataa gcgactttct ggatccttca gagcttcagc gacatatcta tccacaactt
    52681 ctctcgattc atgttcctct gaaaatgcct gaaattttaa ttgactaatt tgatcctgat
    52741 acgaactatc tgctaacaaa acttcaagat gggaaacatt tgctaacgga taaggtcttc
    52801 tatccttacc taaccaagtt tctgtctctt catcctcgat tagtccccag ttactggcaa
    52861 agtcaggatg attctctaaa aaaatacgtt ctgtctgaaa agtgactgac cgaatgggga
    52921 aagaagctgt ttctctctca aaactagtaa acaatgcacg cgcaatcccc tgacggcgat
    52981 gacctggatg aaccaatatc gtcacttcta catcttggtc atctgcatag acagttaata
    53041 aaccaacaag tttgcctttt tcataataaa ggaaaaaggc gggcatgttt gggtcaaaat
    53101 taagcatgtt agagagatag ggatcgcgat aggtaccgtc atagttttgg caacagttaa
    53161 ttactttttt cgcctcagat agctcctctt ggcttaactt gtttcttgct tgaatcatat
    53221 aggtatcctc tacaaaccag acgatctgtg actggcatct ttagcctgct cgagtttatt
    53281 gacataatac tcccgttttt cttcgacttc gtgaataaca ggctcatctt tcttaccatg
    53341 aagacggaca atcttggccg gaataccgac aaccgtcaca tcactaggta catctgctac
    53401 gacaactgct gcagcaccga ccttggcatt ttcaccaatt tccacaggcc cgataacttg
    53461 ggcatgggct gatatgaggg ctccctttcg tacagtcgga tggcgtttgc cacagtcttt
    53521 ccctgttccc ccgagagtca ctccgtgata gagaagaacg cctttttcaa caatcgctgt
    53581 ctctccaatc accagaccag aaccatggtc aataaagaca cctgaatcaa tctgggctcc
    53641 tggatgaatc tcaatctgag tccaaaagcg ccaaaactga ctgtgcatac gagctaagag
    53701 tttgaagtcg tacttccaga gaaaatgcga gagacggtgg gccgccaagg ccttgacacc
    53761 tggataagtc agcaaaacct ccaaagtggt gcgggccgct ggatcatttt cttttacgat
    53821 atcaatggtt tcgtgccacc accccataca tttctccttt tcttactctg aatcttttgg
    53881 tgtttctgta aattctttct taggtttgtg atccttgtga tgacgtgggc ggtgagggcg
    53941 ctcagacttt tcaccttttt catcatgctc aggttttggc ggacgaggta gaagagcctt
    54001 catagaggca tcgatacggc ctttttcatc aatcttgata accttaacat caacttcatc
    54061 cccgatttct accaaatcct ctacacgatt ggtacgagtc caagccatct cagagatatg
    54121 aacaagggca tctgtcttat caaagaggtt aacaaaggca ccaaatttct cgatacgaac
    54181 gactttagca cggtaaactt catccacttt ggcttcacga accaaaccag caataatttc
    54241 tttggcacgg ttaatagcat cttggtcact agagtagata gacacatttc cttcttcgtc
    54301 tatatcaatc ttaacacctg tttcagcgat aatcttgtcg atggtttctc cacccttacc
    54361 gatgacaatc ttaatcttgt ccacatcaat cttgatcgta tcaattttcg gagcagttgg
    54421 agccaattct ggacgaactt ctggaatggt tgcttcaatg acatcaagga tttcaaaacg
    54481 cgctttcttg gcttgagcaa gagcctccgt caagatttct gcagtaatcc cttgaatctt
    54541 gatatccatt tgaagggctg taatcccatc acgagtacct gcaaccttga agtccatatc
    54601 tccaaagtga tcttccaaac cttggatatc tgtcaatact gtgtagttat ttccatctga
    54661 gataagtccc atagcaatac cagctactgg cgccttgatt ggcacaccac cagtcataag
    54721 ggcaagagtt cccgcacaga tagaagcttg agatgaagaa ccgtttgatt ccaaaacttc
    54781 tgctactaga cggatagcgt atgggaattc ttccaagctt ggcaagactt gagcaagagc
    54841 acgctcacca agggcaccgt gaccgatttc acgacgacct ggcgcaccgt aacgacctgt
    54901 ttcccctaca gaatattgag ggaagttata gtggtgcata aagcgtttct tgtactctgg
    54961 atccaaacca tcaatgattt gagtttctcc catcggagcc aaggtcaaga ctgaaagagc
    55021 ttgagtttgc ccacgagtaa agagacctga accatgtaca cgaggaagga agtcaacaac
    55081 cgcatccaaa ggacggattt catcgacctt acgaccatca ggacgcacct tgtcttctgt
    55141 aattaaacgt cgcacttctg cgtgttccat ttgttccaag atttcagcca catcacgcat
    55201 aatacggtca aattcttcgt ggttcgcata tttttcttcg taaacggcag tcacttggtc
    55261 tttcactgct tgagttgcag cttcacgggc caatttctct tctacttgaa ctgccttttg
    55321 gaggtcactg ttgtaggctg caatgatttc agcttgcaat tcagcatcca cgtgaagcaa
    55381 ttccacttct gctttttctt taccgacagc agcaacgatt tcttcttgga aggcaatcaa
    55441 ttctttgaca gcttcgtgcc ctttaagaag cgcttccaac atgatttctt ctgacaattc
    55501 tttggcacca gactctacca tgttgatagc gtgcttggtt ccagctactg tcaattcaag
    55561 aagagattgc tctgcttgtt cttgacttgg gttgatgatg atttggccat ctacatatcc
    55621 cacttgtacc ccagcaattg gtccgtcaaa tggaatatct gaaatagaca gtgccaaaga
    55681 tgaaccaaac atagcagcca ttggtgcaga tgcattttca tcataagaaa gcactgtatt
    55741 gatgacttgg acttcattac ggaaaccttc cgcaaacata ggacgaatcg gacggtcaat
    55801 caaacgcgct gtcaaggtcg catctgttga aggacgtcct tcacgtttca taaagccacc
    55861 aggaaacttc ccagccgcat acattttttc ttcgtagttg acttggagtg ggaagaaatc
    55921 cccagttgcc attttcttag acataacggc agcagtcaag acagttgact caccgtaacg
    55981 tacgacaaca gagccatttg cttgcttagc aacctgacca gtttctacaa ttaactcacg
    56041 acccgcaaaa gtcgtttgaa acacttgttt tgccatttta atcccctttg gattgatgaa
    56101 attatacgcc ttgcctacaa agatcaagat accaaggacg tcaaaagcaa agtaaaaata
    56161 ggaaactgac gaagtcttcg atgaagacaa gacagtttat cttttttaca cagcttttcg
    56221 gccgtgttca attacacaag atattttgga cggttcggct tgccgaacat ttctgtagaa
    56281 aaataggaag gtgacgtcac actcgacgag tgctaggaag cttatctttt ttcctaagaa
    56341 atgagaccaa aattcaagtc atcaagatac caagccgtca agcaactcaa aggaagatag
    56401 gaaatcgaac gacggagcga ctactcctag ggagatttat ctttttccac agagttgtag
    56461 gcaagttcag ttttcaagat acatcattag aaaggtttaa tactaaagta tctaaagctt
    56521 tcacgctaat cgctatcggg cgattagcta aatgctttac taactctctc gtcaaataac
    56581 atcgatttga ctcactcgtg tcgttaaatc ttacagttta aatgcattgt attatttaat
    56641 accttcatct ttgtatcaag tacgtacaga atttatttta tcatattttt cttaaaaagt
    56701 gaggtcttta ccattaaaaa ggaaccattc ccctcacctg agaagaatgg tttgctttta
    56761 ttatcctaga gactggtgat taaacaaggc atgggttgct tgatggatgt attttgctgt
    56821 atcagcatta ttcatcgtat agagatgcac accggcaaca tcctgagtta ccaagtccac
    56881 gatttggtcc actgcatagg caagtcctgc tgctctgagc gactcagggt catgctcata
    56941 cttgtctaag atggctttaa atttgcgtgg aagatggata ttctcacaag tcttcaagag
    57001 tcggagagcc tgatttcgat tcagaattgg cataattcct gcatgaatgg gaacatcaat
    57061 cccagccaag atacacttgt cctgaaaatc atagaagcgc tcattgtcaa agaagagctg
    57121 agttacgagg ctcgaacagc ctgcatccac tttcttctta agattttgaa tatctgaaat
    57181 ctgatttggc gaatctggat gcccttctgg atagcaagct ccaataatat caaagtgagg
    57241 ggcttgttcc ttgataaact caatcaagtc ggttgcatag cggaaatcct tttgtggttc
    57301 tacgtctgga ataatatccc cacgaagagc caagattttc tgcaccccaa ctttgtccaa
    57361 gtcagcaata gtttcagcaa ccttgtcctt agttagataa atagctggca agtgggcaat
    57421 ggtcggaatc gccaagtcat tttggataaa gtcagccaaa cgaaccgtcg tttccttgat
    57481 attaaattta ttattgctgg cagttacact gataaaatgg ggagccaact cctgcatatc
    57541 ctgcaaggct gaaataatgt taccattacc cacggctggg tttggaggga acacttcaaa
    57601 tgagagtgac ggtgtttggc gtgacatatg taataacctt ttctagttga tttctttttg
    57661 aacaaccact gtatggagag aaatccaatc ttacaatttc tcacgcgcag ctttagctgc
    57721 ttcaacaagg cggatcaagc tttcttttgt ttctgggata ccacgtgttt tcaaaccaca
    57781 gtcagggttg atccaaactt tcttgcttgg cactttagca aggatggctt cgattgtgtt
    57841 gtcgatttcg ccttcatttg gtacacgagg tgagtggata tcgtaaaccc caggtcccac
    57901 ttctgtttgg aagtttttcg ctttgagttc gtccaagatt tcaaggtttg aacggctagc
    57961 ttcaaaggaa ataacgtctg catccatgtt atcgatagct gggatgatat ctgtaaattc
    58021 tgagtaacac atgtgagtgt ggatttgtgt gtctggcgct actgttgagt gtaccaagcg
    58081 gaaggcagga attgcccagt caaggtagtc ttcgtaccag tcgctacgac ggagtggcaa
    58141 tttttcacga agagcagcct catcgatttg gataatcttc acaccagcaa cttcaaggtc
    58201 aagtacttca tccttgatag caagggcgat ttgaagagtt gaatccttga tagagatgtc
    58261 ttcacgtggg aatgaccagt taaggatggt aacaggtcca gtcaacatac ctttaacagg
    58321 tttgtttgta cgactttgtg catagctaga ccatttaaca gtgatagggt taagacgagt
    58381 gacatcaccc cagatgattg gtggttttac cccacgcata ccgtatgatt gtacccatcc
    58441 atttttagag aagaggtatc ctgacaagtt ttgaccgaag tactcaacca tgtcattacg
    58501 ctcaaattca ccgtgaacaa ggacatcaaa gtcaatatct tcttgccact tgatccattc
    58561 gtcaatcgtt tcagcaagga aagcatcgta ctctttttga gacaattcac ctttacggta
    58621 agccaaacgt ttggcacgaa cttcttttgt ttgagggaat gaaccaatcg ttgttgttgg
    58681 aagagctgga agtttgaaag cttcttcttg gatagcttca cgttctgcaa aggctggcaa
    58741 acgagtgtag tctgcgtctg tcaagccagc gatacgcgca cgaagttcag cattttcacc
    58801 aacacgctca gtcgcaaaga gttctttgtt ggctgcaaga gcttctgaac cttgaccatt
    58861 gcggatagca tccaagtcac ggatttcatc caatttttca actgcaaagg caaagtggtt
    58921 caagagtgct ggttcaaatt cttcattagc agttgtaaat ggcacatgaa gaagtgagca
    58981 agagcttgtc aaaacgatgt tttcagctgg gatttgctca agaacagtca agctcttttc
    59041 gtagttgttg cgccagatgt ttttaccatt gacaatacct acatagagag tcttgtcagc
    59101 tgggaagcca cctttaacga gttcaagagt tttcttacct tcaacaaagt caagaccgat
    59161 agcatctact ggtaagttta caaggtcagc gtatacgtca cgaacatcac cgaaataagt
    59221 ttgaagcaag acttcaagac cttttttgtc agccaagagt ttgttgtaaa ggttcaagaa
    59281 gagagctttt tcttcagctg tcaagtcttt tacaagagcc gcttcatcca attggatgcg
    59341 agtcgcacca agttcagcca atttagcaaa aacttcttgg taagcagcca ctaagctatc
    59401 tacgaagtcg tctgctttca cgccttcttc aaagtctgac aattgaagga aagtgaaggg
    59461 acctacaaga acaggacgag tgttcaatcc aagttctttg gcttcttgga actcatcgaa
    59521 aatcttgtga ccagccaatt ttacttgagt gtctttttca aatttaggaa cgatgtagtg
    59581 gtagttagtg ttgaaccatt tcttcattgg aagggcgcga acgtcccctt tttctccctg
    59641 gtaaccacgt cccaaagcga agtagcgctc aaggtcagac aagtccaagt tttgaacgga
    59701 tgcaggcacc acgttgaaaa ggaaagccgc atctaggaag ttatcatagt gagaaaagtc
    59761 atttgatgga atttcagtga tgcctttttc tttgacaatg ttccagtgtt tagcacgcaa
    59821 gtcttttgct gctgctaaaa gttcttcttc tgagatttct tttctaaagt atttttcagt
    59881 tgtaaatttt aattcacgga attcgcccaa acgagggaaa ccgatgattg tagttgacat
    59941 gatgtgtcct ccaaaatttg ttgttgaaac tatcttaaca gaaaagaaag cgtctgtata
    60001 attgtaaaaa attagggttt gatatagttt gaaactatat atctgtttcg gacaaaagaa
    60061 aaagacttga agcaaacgtc tcaaatcctt tgtatagctt attttatagc tgtattttag
    60121 ttagaatgct caaacgcgtt attagtaatt cttataagtg actatggctt gttattagaa
    60181 aagactataa ctgattctag tcaacttttt ccctgttcaa gtgggacgat tgctagtgtc
    60241 tttcctaaac tggctaggac ttttaagact gtatccaact gaggactagt ctttcctgtc
    60301 tccatcctag ctatgacagg ctggcttatt ccactgactt cttccagctt tttctgactg
    60361 attccttgtt catacctagc ctcaatcaac tcgctcatga tagccactcg catatcactt
    60421 tcaaggattt cctccttgct aaagagctca gatggacatc cttccaatta ctcccaatag
    60481 cactattctt catcacttaa ccctcttttt tttacgtcta tgtattttta aaaaaatgag
    60541 cgaattatga ttcgatagat tgatcagcgg gtttaaaatt gggactaact tgtttcttaa
    60601 cagattttcc ttttctagga taaagcaaat cctgtttgct taaccccaac tttccatgat
    60661 gaatccaata gtaaatggtt gaaattccca cgttaacccc tttagccatc accatcattt
    60721 caggcaaaaa tttttggtta tgtatagtgg agaatctttt cctttagttc cttggtcaag
    60781 cttgatttct tgaccgagcg cttgcgattg ttttcataag actgttgagc gtagtcggca
    60841 gaataaacct ctttgaagcg cccttttcca agacattttc ggactgtccc acgcttgatt
    60901 tcagtgtgga tagtttgagg agcttttcca agtagagagg caacttctct atttgatttt
    60961 ccttcttttt tccatctttc gattaagcga cggctatcga ttgtcaaatg tttacctttt
    61021 gtagtataat tgtcttgcat ctctgtgcct tttaatcatt tcaatcttaa attggacttt
    61081 ttttacttgg gttgtactta atctataagg aagacaagaa aaagaatatc aatcaagtaa
    61141 agtcacaaag tcacattagc tccgagcaac cattgcaaat tgaggtactc acacaatgat
    61201 taaaacattt ctctctgccc tttcggtcat tctctttgtt atccctatca taacttattc
    61261 tttttttcca tcttctaatc ttaatatttg gctatctacc agacctatct tggcacagat
    61321 ttatgccttc cccttagcta ctgcaactat ggctgccatt ttaagtttcg tatttttttc
    61381 cctatctttt tacaagaaaa ataaacaaat acggttttac tctggcattt tgctcttatt
    61441 atcgctcata ttactattat tcggaacaga taaaaccctt tcttccgcat caaataagac
    61501 taaaacctta aaattagtaa cttggaacgt cgctaatcaa atagaagcac aacatattga
    61561 gcgaattttt agccattttg acgccgatat ggctatattc cctgaactag ctaccaatat
    61621 cagaggtgag caagaaaacc agagaatcaa actattgttt catcaagttg gactttctat
    61681 ggccaactat gatattttta cttctccacc tactgacagt ggaatagctc ctgtgactgt
    61741 gattgtcaag aaaagttacg atttctatac aaaagctgaa acttttcata caacacggtt
    61801 cgggacaatt gtattacatt cgagaaaaca agatatacca gatatcattg ctttgcatac
    61861 tgctcctcct ctgccaggtt taatggaaat ctggaagcaa gacttaaaca tcattcataa
    61921 tcaattggct tcaaaatatc caaaggctat tattgcaggt gattttaatg caactatgcg
    61981 tcatggagca cttgcaaaaa taagctctca tagggacgca ttaaatgcac tgccaccttt
    62041 tgaaagagga acttggaata gccaaagtcc aaaacttttt aatgcaacga tagatcatat
    62101 tttattgcct aaaaaccact actatgttaa agatttagac attgtaagtt ttcaaaactc
    62161 tgatcataga tgtattttta cagaaatcac attttaatta ttttatataa aatcacccct
    62221 ctagtgttca taaactagag ggggaattta tatcctacta tcgtttaacg cacttctcca
    62281 ttcacttttt cttcgagcga tgcttggatt ttttccatat agcgtgcgac ttcttcgtcc
    62341 gttaagctat cttctggatt ttggaaggtc aagctataag ccatggactt cataccaagt
    62401 cccaatttct cacctgagaa gacgtcaaag agtttgatat ctgtcaaacg tttcacgccg
    62461 gcagcttgga tagcatctac aacttcttgg tgagttactt ctgccttgag gagaagggca
    62521 acgtcacggc tgactgctgg gaatttcgtg atttccacaa atggaacagc aggttggagc
    62581 gccccttcga tggctgaaag gttaagctca gctacatacg tttctggaat atcgtaagcc
    62641 ttggcagtga ctggatgcac ttggccaaga aaaccaagaa cttggtcacc gagtgaaatg
    62701 actgctgtac gtcctgggtg aaggctggca atctcagctg ttgctgtata ggtcacttct
    62761 agtcccaagc gagtaaagag cgcttcaagg attcccttag catagaagaa atcaactgga
    62821 actgctgctg tttggaaatc tttttcagca accaagcctg tcaaggcaaa ggcaaaactg
    62881 ttgatttcat ttggaagttc ttcttttgga ttacctgttt gttcaaagac ttttccaatc
    62941 tcgtaaaggg ccaagttttt attcttacga gccacgttgt aggcaacggt atcaaggata
    63001 cctgaaatca tattttgacg gaggactgaa cgatccacag tcattggcca catgagttcc
    63061 gtaaggttac ttggttgagc cgtaaactca actgcttttt caggagttgt tagagtatag
    63121 gtgatgattt ctgtcaaacc tgctccttca gcaatggtac gaacttgacg gcggagtttt
    63181 tgtgtcacag tcaattcacc agctgtacca tcgtcttttg gaaggctggt tggcaagcgg
    63241 tcatatccat agatacgagc gatttcttca aagagatcag cttcgattgt gatatcccaa
    63301 cgacgacgtg gtacgctgac tgtaaagctg tctgcatttc cagaaagacc aaagccaaga
    63361 cgacggaaga cgtcttcgac atcagcataa gacagctcag ttccgaggac acggttaaca
    63421 tcagcaagag ttgaagaaac ttctacatct gaggtatcaa gctcacccgc tgaaacgata
    63481 cccttacgca ccgtcgcacc tgcaagttcc gcaatcaggc tagctgccgc atcaagggct
    63541 tcattaactg ttgccacatt aattcctttt tcaaagcgag aagatgactc agaacgaagg
    63601 ttcaggcgac cacttgtctt acggatagat ttgccattaa aaacagcagc ttcaaggaca
    63661 acacgactag atttttcaga gatttctgtt gcttgaccac ccatgacacc tgcaagggct
    63721 actggcttgt ctgcgacagt gatgactagg tcattcacgt ccaagtcacg ttcttcaccg
    63781 tccaaggtca ccaatttttc accagcacgc gcttcacgca cacggatgtc agtcccttca
    63841 aagttatcca agtcaaaggc atgcattggt tgaccaaaat agagcaggat gtagttggtc
    63901 acgtccacta cgttattgat gggacggatt ccttcgttca tgagaaggtt ttgcaaccat
    63961 tgtggacttg gtgcgatggt cacattgtcc aagatacgag ctgcatagta aggcgccttg
    64021 tctgtctcaa tgctgacaga aagggcatct gccgcagctt cattagtttc tgttagagta
    64081 aattctttaa agttgactgc cttgtcatag atggctgcca cttcgtgagc cactccacac
    64141 atagaaaggg catctgcacg gtttggtgtg atggaaagtt cgatgatttc atcatccaag
    64201 tctaggtaag aaaagacttc ctcacctggc acggcatctt caggcaagat ttggatgcca
    64261 tctgcgaatt ccttaggcac aactgagtca gaaattccca attcaccaag tgaacagatc
    64321 attccaagtg actccaaacc acggattttt ccttttttga ttttgtagtt atcagcgata
    64381 cgagctcctg gaagagccac catgaccttg atcccagcac gcacatttgg ggcaccacaa
    64441 acgatctgac gctcttcttc gccaacgtta acctgacaaa catggaggtg agtctctggc
    64501 acatcttcgc aagacaagac ctcaccgacg acaatttttg agagaccagc agctggtgat
    64561 tcgacaccct ctacctcgat tcctgtagtt gacatttttt cagccaactc ttgtgatggc
    64621 acatcaatgt ccaccaattc ttttaaccat ttataagata taagcataat ttagttctcc
    64681 agaatgacca ttgccacact ggttcttttc ctttcctatc atttcaatag aagaatcctc
    64741 ttcttacctt aatttctttc tcagtaacca atccgtatct actttttgac caaccataaa
    64801 atgatgttgg ctaaattttt caaaaccata tcgattataa aacgcttgag cttttgtatt
    64861 atgctcccaa acacctagcc aagcccaaga aaaactattt tttgtagcaa gttcaagtgc
    64921 gaattcaaac agttgcttac ctagtccaaa tccttggaat ttttgtagca catagaggcg
    64981 ttgaatttca aaagcgtcct ctaattctct ctcagtttga gcacttcccc agttgacttt
    65041 gagaaaacca gctatctcct cctcatgcat aatgaaatag gtttcagagt caggatttcc
    65101 caactcagtt gacacagttt tcagactata agcctcttca aagtattcct gtaactgctc
    65161 ttccgtatta tcatacgcaa aggtttcacg aaaggcttgt ttggcaattt tagccaacac
    65221 ctcaacatct gccatttcta cttttctaat cattatttaa actgttctga gaagcggaca
    65281 tctccttggt agaatccacg gatatcgttg attccataac ggagcatagc tacacgctct
    65341 tgtccaagac caaaggcaaa accagagtat acagtcgcat cgataccact catttcaagg
    65401 acacgtgggt gaaccatacc ggctcccata atttcgatcc aacctgtttt cttacatacg
    65461 ttacagcctt ctccaccaca cttgaagcaa gaaacatcca cctcaacaga tggctctgtg
    65521 aatgggaagt aagatggacg caaacgaatt tgacgctctt caccaaacat tttttggaca
    65581 atcaactgaa gcgttccttg aagatcagcc atagagatat ttttcccaac taccaagcct
    65641 tcgatttggt ggaattggtg actgtgggtc gcatcgtccg tatcgcgacg gaagacacgc
    65701 cctggcgaga tcatcttcaa aggaccttta gaaaaatcat gggcatccat agcacgcgcc
    65761 tgaactggag acgtgtgggt acggagcaag atttcttcag tgatatagaa agtatcctgc
    65821 atatcacgag ctgggtggtc ttttggaagg ttcatacgtt caaagttata gtagtcttgc
    65881 tccacttcaa aaccatccac gacttgataa cccataccga tgaagatatc ttcgatttct
    65941 tcactggttt gtgtcaaaac gtgacggtga ccagtcgcaa ctggacgacc tggaagcgtc
    66001 acatcgatac tctcgctagc cagttgagcc gcgactttct tttcttccaa gagcttagct
    66061 gtttcttcaa aagcagctgt caagacatca cgagcttcat tgacgtgttt cccgatgatt
    66121 ggacgcatct cagcagaaac atctttcatc cctttgagga tttcagtgag cgaacccttt
    66181 ttaccaagga cagagacacg caaatcttgc atctcttttt catttccagc agtaatctgc
    66241 ttcaagctag tcagcgtttc ttcgcgaagc gcttttaatt gttcttcaat agttgacata
    66301 tttcctccat cagtctctcg tagataaaaa gaaaaccaca tgccaaaaac tccactcgga
    66361 gcgttgacac gcggtaccat ccgttttcat ctgacaagtc agaccttcat ttctaaatcc
    66421 atgcgcaagt gaattcaccc agctttcata tagagagctt gcagtcacgg ctctcctccc
    66481 tgatatactt cccttgggct actagtcttt cagatttcta ttcaattacc acttagttta
    66541 tcagattttt accattcttg caagacctat cttacttctg cttgttagct tattcttatc
    66601 taactttata gcggattgaa ataagatgaa aacaaatcga ttgggaaagt aaaattaatt
    66661 tctataaatg ttttagcaat tgtttcgtac tattttagat tcagtctact atatataacc
    66721 aatctatcct aagttaacta tttataatcg gctctctgta acaaaattaa attaattaat
    66781 ccccctataa aataaagagg tttagaattt gatgtcttcc aaacctcttt atttcatatt
    66841 taatgagatg ctgccttaac cgtgataata tctaatcatc aataaacaaa ctatttgaat
    66901 aaggattctc catttgattc aatcactcct ttataccaag taaaggacat tttcttatat
    66961 cgattgaatg tgccatttcc atcatcattt cgatcaacat aaatgagacc gtacctctta
    67021 gaaagctgtg cagtggacat agaaacgcag tcaatacatc cccaagacgt atagcccata
    67081 atttccacac catcttgtag agcttcagct acttgcaata aatgttcttt catatactga
    67141 attctataat catcttggac tgttaagtta ttaaattcat cttttattag ttgatcttta
    67201 gcacctaatc cgttttccac tataaataat ggaatttgat aacggtcata atatctattt
    67261 aaaattatac gtagtccaat tggatcaatt tgccatcccc actcagaaga ctctaaataa
    67321 ggatttacta aaccaccaat aatattccct tctcctgaat tatactgtgt tggaagagca
    67381 gattgagtca cactcatgta atagctaaag gataaaaaat ctacagtata attttttaat
    67441 aactctgcat cttcagctgc aaactctatg ttaatgtcat tttccttaaa atatcttttt
    67501 gcataattcg gataataacc tctaacatgc acatctgaaa atagataatt tagattctca
    67561 tactcatgag tcgcccatac atcttttgga tttggagtca ttggataagc tggcatagct
    67621 aataccatac accccacctt aaactctgaa ttaatctcac gagcaatttt tgtaaccaaa
    67681 ctcgaggcga ctaattcatg atgtatagct tgatataatt cttgtttcga aagattctcc
    67741 ttaggtatat ctattcctcc actagtaaac ggtaattcca aaacagagtt tacttcgtta
    67801 aatgtaagcc aatatttaac tttatcttta tacctttcta aaactgttcg agcaaatttt
    67861 tcataaaaat gaatcattct cctatcaatc catccatgat attttcttgc taaatataat
    67921 ggagtctcat agtgtgaaag agttacaagt ggttctatcc cgtgagcatg tagttcatca
    67981 aacaattcat cataatattt caacccagct tcgttaggtt cttcctcatc tccttttgga
    68041 aaaattctac tccatgcaat agaagtacga aaaacattaa agcccatttc agaaaacaag
    68101 gatatatctt ccttatattt atgataaaaa tcaataccta tcaattttaa gttatcttct
    68161 gtaggatttt ctgttgcttc tcctaatcca cctttgggta acacatcctg aactgataag
    68221 cccttaccat cttcattata tgctccctct acttgattag ctgcaacagc tccaccccaa
    68281 agaaaatcat ctggaaaaat ggtcataact ttcctccatt ataatattac cagtaattcc
    68341 ttagaatgct cgattgtctg attattaggt aatactaata catctagaaa atcattggta
    68401 ttcgttacaa ttactggtgt aactgtttcg tagcctttag tcttgattaa attcaagtcc
    68461 atttcaaaaa tcaactgatt tttgaaaact ctgtctcctt cttctacatg actaataaaa
    68521 ccttgacctt ttagctcaac agtatctaat ccaatatgaa ttagtaactc aacaccctca
    68581 tcactcttca atccaattgc gtgcttagtc ggaaaaatat ttgtaatttt cccatcaaat
    68641 ggtgcataaa ccttaccttc acttggaata atcgctactc cgtctccaat tagtttatct
    68701 gaaaatgttt tatcctggac atcgcttaac ggaatgattt ctcctgatat aggagaaaat
    68761 atcatttttt tatttgaaac tccagcttta acttctaaat tgctagaact ctcttcttca
    68821 tcgattccaa atatataagc taatacaaag gtaataacaa ccgaaataac cgccacaatt
    68881 aaagcattta caatatttga tggtacatca gaataaataa attgaggcaa cgctatcaaa
    68941 gatgggacag caaatagata cgctttaaca ctagtaagac ctgcaaataa tcccgctaat
    69001 ccaccaccaa tcatagctgc ataaagcggt tttttatatt ttaaagtcac accatataat
    69061 gcaggttcgg taatccctgc aagtaaggct gagaaacctg ctgcaaaagc aatttgtttt
    69121 gtattattat ttttactctt taatgcaaca gccatcgaag cagccccttg agctaagttt
    69181 gaccctaaca ttgctggaag aattaatacg tctggagtag caatagatgc cgccaaaaaa
    69241 ataggtgcaa aagcccagtg cattccagtc ataacaataa atggcataat agcaccaaga
    69301 atagctaatg taagccatcc agctacatca tacatttgcc caactagatt tgataatcct
    69361 tcaccaacaa ttactccaat aggcccgact acaactaagg caatacagct tgatactaat
    69421 aatactagcg taggttgcaa aaaactctta gtaacagctg gtgttaattt agcaatcatt
    69481 ttttcaatat atttcatcaa ccaaaccata ataagaattg gaacgactga cgaaccataa
    69541 ctagctggta tcacaggtac accaaataaa ctaagaggat tccctgattg caccatttga
    69601 acaaaatttg gatggagaag tacacctgct acagacatag ctaatgtaga tgttactttt
    69661 aatttttgtg atgcagaata agctaataac agcggtaaga aataatatgg agcatcccca
    69721 aaaaatgtca aaaaagcaat agtctgagaa tctgattgca atataccaag cattggtaaa
    69781 ataattacca agactttcag catacctccc cctaacattg ctggaatgat tggagtcatg
    69841 gaaccagcga tatactcaat gattctttct aaaatattcc ctttgtgccc ttgaacaact
    69901 gaatcggatt caaaattgcc aagtttaacg aattctttat aataattagc tacatcatta
    69961 ccaagtataa tttgatattg tccattcttt ttcataatac ctattacacc tggtatcttc
    70021 ttcacatcat catcattgac taaattttca tcttttaatt ctaatcttaa acgtgttaca
    70081 caatgggtaa ctctattgac atttttttca cctccaatta catcgaggat tttttgtacc
    70141 ttatctttat aactcatggt attctcctat tctattaatc taaatttttt gttaagcgac
    70201 gaatatgagc catcaaataa actaattcac tagaagtcag caaataattg tactcagttt
    70261 gtataaacat tgccacctgt tcaccacatt catattctct aggatattta tttttcatta
    70321 atgctaacaa gtcttcatca tcatcgtcac aataagttgt attttcaacc aagcgatggg
    70381 caaataattt tatatgagtt atgaatctat aataatcaat agaatcttgg ttaaactcag
    70441 tacaatagaa atcttgaatg attttctcaa ttcccataat tatttcagtg attttatacg
    70501 actcttgaaa attattttct aaattagcat taacaaaatg tagcgctatg aatgcagctt
    70561 cgtcaattgg aagttcaata cctaactctt tttttattat ttgtaaagcc cttttcccaa
    70621 gctcaaattc ttctttataa tatttcgaga tttcccagcg taggggattc gaaataataa
    70681 ctccttcttt ataacgttct atactcgaag aaatatgatc cgttagattt atatagataa
    70741 tctcatcaaa attattacct aatgttattt tacctagatt aattattttt tcggtgatgt
    70801 acaccaactc caaaggaacg ttaataaaca tctctgtaaa acgggtcata tcatctctat
    70861 ttttgagaaa aaaacttttc tcaatatcaa ttggatttac tttatctcca atttttcttc
    70921 caaaagctat tccccttcca aaaagaagaa tatcgatatc ttttttactt tgcgcaatta
    70981 cggcattgtg gtttagtatt cgttttatga tcataaaatt tcccttttaa aataaaagga
    71041 acctacttaa aaacatcaag aaacttcttc tgatgtgatt aaataggttc ctgctcactt
    71101 gtattagtga taacatcctt ttatttcaat ttttaatggt gacatgaagc ttaaataatt
    71161 aataactctg taaccatttt cattttaaga tatttcaatc tatttgtcaa gtacttgtag
    71221 aaattttttt attttgtgtg attcgaatgt gataatggca acagtctcac taaagaaatg
    71281 aaaatcatac atagaaattt ttaaaaattt tctctatcgt aaacctgcca ctagttcaga
    71341 aactatacta gctgattcca aacgtaatat ggtcatctca tctaccttcc ctaataatgg
    71401 cattgcatca taccaaacaa tagtcttatt caccaaaaga ataggcgtta ctatttttga
    71461 ttgagtaaaa gacactttaa ttttctggct cttcaactgg tctagccatg ctttatttgc
    71521 aaccgtctct ggtatacata tctcaatttg actaatccta aattctttta gaaaattcat
    71581 cagttttgtc tggtgcacat aaggtaaaat aagcagacat tcctgtcttt cccctgctaa
    71641 gtcctctatc aacaccttct catatctact atcaacataa acgaattgtt tctcctcacc
    71701 ctcaatggca cgataatcca tctttcgata agctacttgt cgtttctgaa acatcttttc
    71761 taaataagga acatgaatat ccacataatc gaaaatccgc accaaagact tatccttgta
    71821 gtttctatga atccgacctg agtactgaat caaattattt ttccaagaaa agggtgctgc
    71881 caagataagc gtgtccaact gaggtaagtc aaaaccttcg ccaatgtatt ttccagtaga
    71941 caacaaaaca aaccctttat ctaactgttc taacgtctcc agtaaactcg ttctctttct
    72001 cggactttgg tttttccgct aataatgtaa cagtcatcaa cctctttctc tttcaataac
    72061 ttttcaaaga catctatctg ttgaattcga ttaactaaaa ccaagatatt ccgtccttct
    72121 gccacttggg ctagaatatc cttgagaatc aactgattcc tcgctgagtc tgtagcaatc
    72181 caatcactga gctgtataaa attacttgct ttggtctttt caatttccaa atgagcaaaa
    72241 gaagtgaatc ttaattgcaa ttgccgttta aaatccgttt ccctcttatc agcagtatgg
    72301 agtatctcac caattctctg aaaaacaata ggctcatgac cattcttacg ctcaggcgta
    72361 gccgtcaaac cgtaaagata cttccctcta aactgagcaa caactttttc aaacatcaag
    72421 gcagagacat gatgacactc atccacaatc atcatctcat actcatccaa aagactttga
    72481 ctattttcca acttaaatag agattgaatc ataacgacat caaccagttt actcagccat
    72541 ttcttagtcc caccgtactg cccgacatag cctattaccc tttcacgacc tgatggtgta
    72601 taacgggtag cttctgcctc ttcgaaagtc aaaaaacact ttaaacgatc cagtcattgg
    72661 tctaagagtt gcctattatg aactaggata atggtttttg tcttacgttc agagataaga
    72721 gcagcaccta aaacgctctt tccaaaacct gtcccgcatg aagtaaacca ttttctttag
    72781 aagtcatatc tgacagggtt aactcttgct caaaagtgag ttctccttta aattccacgc
    72841 taatagacct ttgtaccttt ctcctatctt ccacaactac ctgcttaaat ttatcttgca
    72901 atggatataa caagcctctt ggcaaccata aataatgatc ggattctcca aataaataca
    72961 ttctctcagg aatttgatag gttggctgtc gcatagcctg ctttaaataa aattcgggat
    73021 tagaaaatga agccatattt ttcaagaaaa acagtgtctt gggtgtcaca gacgattttt
    73081 caagttggat catattggat aagacgatct tcaacccctt gtccaattct tgcttgccta
    73141 actcttcttg gattaacagt gccactttag cagttgaaac cctctgaatt tcttgtagat
    73201 acctccattg gtcttcataa ggctgaaact gttcatccac aaagaccgtt cgcccttgat
    73261 ggtaagcttc tccttgaaaa ggcaaggcaa tcaaatttcc aaatcccccc ttaggaagga
    73321 catcctgatt tggaaacatg cgatcaaaag aatcaaagga cagttgcata ctttcctgca
    73381 ttgccagttc tatcagtttc tttccaaaca agcgagcctc tcgactcgga atcgcttcct
    73441 caaagaagaa ccaaatatgg agtccgtgac ccgaacgaga aatctctaaa tgggcttcca
    73501 tctggcgttc cctggctatt cttcgaatgg ttaaaccagc ttctttccaa tctccttcat
    73561 caaaatccag taccaaaaaa tgacagctat catctaagtg cataggaaag ataccgatag
    73621 ctgtctcccc acgaaaatga gatttcaaaa cggagttcgt caatgtctgg aaactccgtt
    73681 tttcaggtgg caactgcttc caaccataat cataggaagg aaaatactga attttccctt
    73741 gttcattgat aaaattctta gcataaacat cataacgtcc agcaaacacg gaggtaaaca
    73801 attgtaattt ttcttgcgtc gtcaagtgac tactttgaag ttggaacatc tcctcgaacc
    73861 gagagcgttc cataacaaac tgactcttgt ctttaagacg tttacacaag aaaaactcac
    73921 tatccccatc aaaagaagaa aagacatcaa gcacttctac cacaattcca tccaaagaca
    73981 caaaaacagc cataagagtc acctccttga ttcctatagg ctgattataa caagaatagc
    74041 tgaaattgta cacgaaaata aaatccgaat agcactcatt ttgtatgcga ctaatagtcc
    74101 atctcgctcc ggaatgtacg aggtaaatag agtttaaacg agcagtctat aaaattcaaa
    74161 aaacgcatat taccaaggtt gttttgaact acaccccaaa agttagacag aaaaaatcta
    74221 actttttggg gtgtttttat tatgaaatta acttatgatg ataaagttca gatctatgaa
    74281 cttagaaaac aaggatatat cttagagaag ctttcaaata aatttgggat aaataattct
    74341 aatcttaggt acatgattaa attgattgat cgttacggaa tagagttcgt caaaaaagga
    74401 aaaaatcg
//
LOCUS       DAVDWT010000005        70338 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVDWT010000005 DAVDWT010000000
VERSION     DAVDWT010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 70338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70338
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.005"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            474..680
                     /locus_tag="VUW50_000400"
     CDS             474..680
                     /locus_tag="VUW50_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982357.1"
                     /translation="MGEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
     gene            677..871
                     /locus_tag="VUW50_000401"
     CDS             677..871
                     /locus_tag="VUW50_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982358.1"
                     /translation="MKRGIIYFFICLSLLVWLVEMFTDWFDQALLRQFIRGALGFGFI
                     IFVVFPMGMEWLKGESHDGG"
     gene            858..1742
                     /locus_tag="VUW50_000402"
     CDS             858..1742
                     /locus_tag="VUW50_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982359.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPMTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEKSVLVENWSQKQDALQVVTREADAFWELLVQAGCRIQEIEV
                     NNRSLLDTIFEETQKGDD"
     gene            1744..2481
                     /locus_tag="VUW50_000403"
     CDS             1744..2481
                     /locus_tag="VUW50_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7982360.1"
                     /translation="MKRWIALNKIEFLLTKRQLVYYLLSVGMPTAFYLFFSGMYQDTP
                     GGPANFMRDYLISMTAFSMMSTAIFSFPAVLHTDKINNWQKTLRHTPVNMVEYYLSKI
                     TSMMVDYLVSILVVFSVGHFVRGVDMPLASWVGAAILLILGSIAFVALGLTLTLLPSS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAIGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            2481..3578
                     /locus_tag="VUW50_000404"
     CDS             2481..3578
                     /locus_tag="VUW50_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7982361.1"
                     /translation="MLERLKSIHYMFWISLIFMIFPILPVVTGWLSAWHLLIDILFVV
                     AYLGVLITKSQRLSWLYWGLMLSYVVGNTAFVAVNYIWFFFFLSNLLSYHFGVRSFNS
                     LHVRTFLLAQVLVVGQLLIFQEVEVEFLVYLLGILTFIDLMTFGLVRIRIMEDLKEAQ
                     AKQNAQINLLLAENERSRIGQDLHDSLGHTFAMLSVKTDLALQLFQIQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLISELETVKKMLEIAGIEVETDNQLDTASLTQKL
                     ESTASMILLELVTNIIKHAKASKVYLKLERTEKELILTVRDDGCGFASIKGDDLHTVR
                     DRILPFSGEVKVISWKQPTEVQVRLPYKERK"
     gene            3579..4178
                     /locus_tag="VUW50_000405"
     CDS             3579..4178
                     /locus_tag="VUW50_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7982362.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLAFQADVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRAEKLETKVVVVTTFKRPGYFERAVKAGVDAYVL
                     KERNIADLMQTLHTVLEGGKEYSPELMEVVMMHPNPLTEQEIAVLKGIAQGLSNQEIA
                     DQLYLSNGTVRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            4497..5507
                     /gene="ccpA"
                     /locus_tag="VUW50_000406"
     CDS             4497..5507
                     /gene="ccpA"
                     /locus_tag="VUW50_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HET7982363.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            5571..5729
                     /locus_tag="VUW50_000407"
     CDS             5571..5729
                     /locus_tag="VUW50_000407"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982364.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHVFEVVDETDEVS
                     NHTYGKAT"
     gene            complement(5758..6720)
                     /locus_tag="VUW50_000408"
     CDS             complement(5758..6720)
                     /locus_tag="VUW50_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET7982365.1"
                     /translation="MPKKILVLRTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKNYMEG"
     gene            6786..8174
                     /locus_tag="VUW50_000409"
     CDS             6786..8174
                     /locus_tag="VUW50_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET7982366.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKIVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDMLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            complement(8209..8661)
                     /locus_tag="VUW50_000410"
     CDS             complement(8209..8661)
                     /locus_tag="VUW50_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7982367.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            8823..9116
                     /locus_tag="VUW50_000411"
     CDS             8823..9116
                     /locus_tag="VUW50_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982368.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMIWQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMTWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            9272..10486
                     /locus_tag="VUW50_000412"
     CDS             9272..10486
                     /locus_tag="VUW50_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7982369.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            10631..10765
                     /gene="rpmH"
                     /locus_tag="VUW50_000413"
     CDS             10631..10765
                     /gene="rpmH"
                     /locus_tag="VUW50_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET7982370.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            11049..11942
                     /locus_tag="VUW50_000414"
     CDS             11049..11942
                     /locus_tag="VUW50_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HET7982371.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANAGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIVMVTVPKRPVAPNAAPKTTSTPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLAAATSGFALLGRKTRRRK"
     gene            12247..13020
                     /locus_tag="VUW50_000415"
     CDS             12247..13020
                     /locus_tag="VUW50_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET7982372.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALADEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYIRYVVDFIADLRGMTTEELAAATTANAERIFGLDSK"
     gene            13020..13580
                     /gene="rnmV"
                     /locus_tag="VUW50_000416"
     CDS             13020..13580
                     /gene="rnmV"
                     /locus_tag="VUW50_000416"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HET7982373.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSCKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            13652..14551
                     /locus_tag="VUW50_000417"
     CDS             13652..14551
                     /locus_tag="VUW50_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7982374.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            14820..16850
                     /locus_tag="VUW50_000418"
     CDS             14820..16850
                     /locus_tag="VUW50_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET7982375.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREKVTEALSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSI
                     LGLGILWYQGALFMATVQLVIIALLLYGLILAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQYSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYFPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            16852..17493
                     /locus_tag="VUW50_000419"
     CDS             16852..17493
                     /locus_tag="VUW50_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982376.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRMIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            17486..17794
                     /locus_tag="VUW50_000420"
     CDS             17486..17794
                     /locus_tag="VUW50_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982377.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            17925..18797
                     /gene="rsmA"
                     /locus_tag="VUW50_000421"
     CDS             17925..18797
                     /gene="rsmA"
                     /locus_tag="VUW50_000421"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET7982378.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFACLADALKGQGL"
     gene            18799..19677
                     /gene="rsgA"
                     /locus_tag="VUW50_000422"
     CDS             18799..19677
                     /gene="rsgA"
                     /locus_tag="VUW50_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET7982379.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFFNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETHKKVSKKIPK"
     gene            19688..20344
                     /locus_tag="VUW50_000423"
     CDS             19688..20344
                     /locus_tag="VUW50_000423"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET7982380.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVEALRPHSKMVFDCHLMVANPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            20307..20969
                     /locus_tag="VUW50_000424"
     CDS             20307..20969
                     /locus_tag="VUW50_000424"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HET7982381.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            20971..22227
                     /locus_tag="VUW50_000425"
     CDS             20971..22227
                     /locus_tag="VUW50_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET7982382.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            22217..23158
                     /locus_tag="VUW50_000426"
     CDS             22217..23158
                     /locus_tag="VUW50_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HET7982383.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            23425..24252
                     /gene="purR"
                     /locus_tag="VUW50_000427"
     CDS             23425..24252
                     /gene="purR"
                     /locus_tag="VUW50_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HET7982384.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            24319..25569
                     /locus_tag="VUW50_000428"
     CDS             24319..25569
                     /locus_tag="VUW50_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET7982385.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            25695..26489
                     /gene="pflA"
                     /locus_tag="VUW50_000429"
     CDS             25695..26489
                     /gene="pflA"
                     /locus_tag="VUW50_000429"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HET7982386.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(26564..27490)
                     /gene="yidC"
                     /locus_tag="VUW50_000430"
     CDS             complement(26564..27490)
                     /gene="yidC"
                     /locus_tag="VUW50_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET7982387.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(27568..27846)
                     /locus_tag="VUW50_000431"
     CDS             complement(27568..27846)
                     /locus_tag="VUW50_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET7982388.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            27880..28620
                     /locus_tag="VUW50_000432"
     CDS             27880..28620
                     /locus_tag="VUW50_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET7982389.1"
                     /translation="MTIITSKANSVVKNAKKLRQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFYLS"
     gene            28676..29359
                     /locus_tag="VUW50_000433"
     CDS             28676..29359
                     /locus_tag="VUW50_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET7982390.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPSALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            29470..30462
                     /locus_tag="VUW50_000434"
     CDS             29470..30462
                     /locus_tag="VUW50_000434"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HET7982391.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            30527..31066
                     /gene="rsmD"
                     /locus_tag="VUW50_000435"
     CDS             30527..31066
                     /gene="rsmD"
                     /locus_tag="VUW50_000435"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET7982392.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAKRELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            31056..31544
                     /gene="coaD"
                     /locus_tag="VUW50_000436"
     CDS             31056..31544
                     /gene="coaD"
                     /locus_tag="VUW50_000436"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET7982393.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            31528..32565
                     /locus_tag="VUW50_000437"
     CDS             31528..32565
                     /locus_tag="VUW50_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET7982394.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            32623..32853
                     /locus_tag="VUW50_000438"
     CDS             32623..32853
                     /locus_tag="VUW50_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HET7982395.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            32877..34160
                     /gene="murA"
                     /locus_tag="VUW50_000439"
     CDS             32877..34160
                     /gene="murA"
                     /locus_tag="VUW50_000439"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET7982396.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            34153..34341
                     /locus_tag="VUW50_000440"
     CDS             34153..34341
                     /locus_tag="VUW50_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET7982397.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            34380..35204
                     /gene="endA"
                     /locus_tag="VUW50_000441"
     CDS             34380..35204
                     /gene="endA"
                     /locus_tag="VUW50_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HET7982398.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            35433..36764
                     /locus_tag="VUW50_000442"
     CDS             35433..36764
                     /locus_tag="VUW50_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HET7982399.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTNTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(36962..37032)
                     /locus_tag="VUW50_000443"
     tRNA            complement(36962..37032)
                     /locus_tag="VUW50_000443"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36998..37000),aa:Cys,seq:gca)
     gene            37448..41059
                     /gene="rpoB"
                     /locus_tag="VUW50_000444"
     CDS             37448..41059
                     /gene="rpoB"
                     /locus_tag="VUW50_000444"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET7982400.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            41092..44769
                     /gene="rpoC"
                     /locus_tag="VUW50_000445"
     CDS             41092..44769
                     /gene="rpoC"
                     /locus_tag="VUW50_000445"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET7982401.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKTEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPTVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            44880..45293
                     /gene="ndk"
                     /locus_tag="VUW50_000446"
     CDS             44880..45293
                     /gene="ndk"
                     /locus_tag="VUW50_000446"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET7982402.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYLPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(45803..46081)
                     /locus_tag="VUW50_000447"
     CDS             complement(45803..46081)
                     /locus_tag="VUW50_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HET7982403.1"
                     /translation="MKKFDNYIIEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(46239..46871)
                     /locus_tag="VUW50_000448"
     CDS             complement(46239..46871)
                     /locus_tag="VUW50_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982404.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(46873..47640)
                     /locus_tag="VUW50_000449"
     CDS             complement(46873..47640)
                     /locus_tag="VUW50_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HET7982405.1"
                     /translation="MSQKLNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRMSSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     VGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            complement(47691..49430)
                     /locus_tag="VUW50_000450"
     CDS             complement(47691..49430)
                     /locus_tag="VUW50_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7982406.1"
                     /translation="MKIMKKKYWTLAILFFCLFNNSVTAQEIPKNLDGNITHTQTSES
                     FSESDEKQVDYSNKNQEEVDQNKFRIQIDKTELFVTTDKHLEKNCCKLELEPQINNDI
                     VNSESNNLLGEDNLDNKIKENVSHLDNRGGNIEHDKDNLESSIVRKYEWDIDKVTGGG
                     ESYKLYSKSNSKVSIAILDSGVDLQNTGLLKNLSNHSKNYVPNKGYLGKEEGEEGIIS
                     DIQDRLGHGTAVVAQIVGDDNINGVNPHVNINVYRIFGKSSASPDWIVKAIFDAVDDG
                     NDIINLSTGQYLMIDGEYEDGTNDFETFLKYKKAIDYANQKGVIIVAALGNDSLNVSN
                     QSDLLKLISSRKKVRKPGLVVDVPSYFSSTISVGGIDRLGNLSDFSNKGDSDAIYAPA
                     GSTLSLSELGLNNFINAEKYKEDWIFSATLGGYTYLYGNSFAAPKVSGAIAMIIDKYK
                     LKDQPYNYMFVKKILEETLPVKNGIKVLNIPNVLRYDLNMLQLEYKNEQSWDSFIDNV
                     NLIELEERIQTTIGIKQINTHNIITIAREGYSQNYLPNTSENTYNSLQVSLVGVLLLF
                     ISMVNILWAKKSK"
     gene            complement(49454..51562)
                     /locus_tag="VUW50_000451"
     CDS             complement(49454..51562)
                     /locus_tag="VUW50_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HET7982407.1"
                     /translation="MKIPMIYQMENSECGLACCAMILNYFKYEISLNELREIYPSSRS
                     GYSLLSISKVLGDFNISSHAFKASVRDLKPLSFPLICFWESSHFIILEKISKNKFYIL
                     DPAKGRQRMSISEFERHYSNIILTFKKLDSFMSRKDNKKSPVLKYFFKYRNKLGILFF
                     VTALLYVIQSLVPIANRYIIDTNFKDDSYSSRMLFTILFIFTVSFSLMYLLRQIYVAS
                     LKYIMDKEISYDFMKHLIYLPYSFYEKRTLGDILFRANSIVYIREILSNNFIAAILDL
                     LMIVVYAVVLFSFSKYMVIFLISLSLALSIVMYPIIKISKNLIDKNIKEKVNVQNITS
                     EVISKNSDIKLTGEEEFWINKWDNFNTKQLIIGRKLDIHLSIVSSITNVLQIILPVLT
                     LIVGVNIKTFEQLTLGQIVAISTVSPYFISPIISLSDNYIQLMLLKGYFLRIEDVFNT
                     KSELIPERVSQDIKFDKKIELKDIWYKYGLFDDYVLKGINVTIKKGETVAIVGESGSG
                     KSTLAKILLGLLEPNIGSIEVDGVEKEEIGQTLYRKIFGAVLQNSTLSYGTLRENLTF
                     GHFVSDEELMTNLNSIGLSNVVKSLPLGLETIIAEEGNNFSGGQQQMILLARCLLSKP
                     SVVVLDEATSSLDNLSQQITTSYLSEIGTTKILIAHRLDTIKSADKILVMHNGEIVEI
                     GTHRELLELGGIYKQLYSNN"
     gene            complement(51559..52488)
                     /locus_tag="VUW50_000452"
     CDS             complement(51559..52488)
                     /locus_tag="VUW50_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HET7982408.1"
                     /translation="MLKILNNSVIYLCLIPLLFLLLIIFPNDSLIYYFRLILISLISI
                     TLHELGHFLVGRCLSYKLEMLATPFFFYFRKKIYFKFPVLLAFGYCQMSNRNITNEKN
                     SDRNLVFYFFGGGGANLIVAILALLGFVPYASEFFILNIILFLVTVCLPIDGTDGNAI
                     REIVLYSKDSKTYQRFFANSLYNNPYITIDDFAKLTSKEKSFFSKFEKCLINLYFEVK
                     GEGSAFTIANHEVFDSNIQENIIHEYYKLLHKSSDWIIPQNLSLEEVVFTLSNYIYSK
                     NNKYLEKIKYLKKLVDFRQEEIIDFILNKEEVL"
     gene            complement(52469..53173)
                     /locus_tag="VUW50_000453"
     CDS             complement(52469..53173)
                     /locus_tag="VUW50_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HET7982409.1"
                     /translation="MNKIFIYAGVRNHNSKTLEYTKRLSSIISSRNNVDISFRTPFNS
                     ELEISNSDSEELFKKGIDRQSNADDGGVIKKELLESDIIIISSPVYLQNVSVDTKNFI
                     ERIGGWSHLFRLAGKFVVTLDVAESNGSDNVSEYLRDIFSYMGGQILHQVSITNSLKD
                     IAEAQLMEATYKIEDVLEGKIKYKTTDYQERAYQTLKLILENYDSEHFEKMYWEKKRL
                     FEANSLEEWYYVENIK"
     gene            complement(53523..54533)
                     /locus_tag="VUW50_000454"
     CDS             complement(53523..54533)
                     /locus_tag="VUW50_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin formation protein"
                     /protein_id="HET7982410.1"
                     /translation="MVINNIYDEYPIWSIPVNFKGSKWCLEQTGNDMYRGRAGILLFM
                     YYVKLLKDKSSYTQLYNKMLSAIPPSVSLSKTTFGLTGDIGYFIVLSIIEDYDTNTLA
                     CLNYIKSVLTELEKTDFASVSNKSDYINGLLPLINTLVRYYRRGIEKERVLRLVLSLG
                     DTLYNDVSERDNSNFGYSFGHGLSGVIFTLKNIYKVTRLKKYKELILQLVPREKIKIH
                     SLKWCSGEFGTLVNHPQSVPEGIVASLDNDTFCHGSMAIINFYIDYMKEMDTSMELES
                     LLCSVVMNKSKEGEYRIVQTSNFPDFSLYTGVTGIAYTFLRYIALNQNDKKIVIPSLL
                     EI"
     gene            complement(54583..56493)
                     /gene="lanM"
                     /locus_tag="VUW50_000455"
     CDS             complement(54583..56493)
                     /gene="lanM"
                     /locus_tag="VUW50_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 lantipeptide synthetase LanM"
                     /protein_id="HET7982411.1"
                     /translation="MHKTLFIKERYQILSERNDNSLNNFSLNNWRSTMSIISEKTFEE
                     MLKLNNYNKKIFSNTVNYDYTEEQIRLYSEYAKNSEWYKFAEGSFEIFRKHPQRINFK
                     KDFSRILEPFIINLLKLINNYLENKEYEWIDKNGNIFSSLVFYLEDLIYPWIVKPLVL
                     EINSLREKGLLEGESEQQRYKYFITLFDKEENILNFYNKYPVLLRQISESCLRFYTYF
                     IEILSNLENDFSVLEEELGLRGKLNDIKFGKGDTHSQGKTVLILFFDDAKIVYKPKNL
                     IINNSLNTIAEYIRKVDEKIRIRIPRTIAYSDHSYEEFIDYLPLEQKKKLPEYYYNFG
                     VLLAFIYLFNGSDIHFENLISYGDMPVIIDFETMLQQPLFDDKTGQSLLDTLFHRVTR
                     TLLLPTEGVKREDGLDVEMSALTGNFKKDAFNGQVLINLNTDKVKFDIGKIDFEGGKN
                     LPVRDGDIEFDKYIKDFKKGFRDFYLIFEELNKTEEFKMLLKANLYGLKTRVLFRDTN
                     SYASVLSFLYHPDFYEEMLDREKALENLWSNKFSNQGIVASECEQMRLLDIPIFYTDT
                     NINEIYDDFGNHFISLHQFSGYDYFMKYLPNNNLENLEKQLDIIEIYFPSFDQRKKGY
                     KLNLSNRKKTPK"
     gene            complement(56631..56819)
                     /gene="pneA2"
                     /locus_tag="VUW50_000456"
     CDS             complement(56631..56819)
                     /gene="pneA2"
                     /locus_tag="VUW50_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HET7982412.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
     gene            complement(56826..57050)
                     /gene="pneA1"
                     /locus_tag="VUW50_000457"
     CDS             complement(56826..57050)
                     /gene="pneA1"
                     /locus_tag="VUW50_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HET7982413.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKVCISAAVSGIGGLVSYNNDCLG"
     gene            57515..58378
                     /locus_tag="VUW50_000458"
     CDS             57515..58378
                     /locus_tag="VUW50_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7982414.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYLYKIEVERS
                     TFLTLEDRTYLEWIKSIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            58761..59381
                     /locus_tag="VUW50_000459"
     CDS             58761..59381
                     /locus_tag="VUW50_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982415.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            59467..59910
                     /gene="tsaE"
                     /locus_tag="VUW50_000460"
     CDS             59467..59910
                     /gene="tsaE"
                     /locus_tag="VUW50_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET7982416.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            59900..60418
                     /locus_tag="VUW50_000461"
     CDS             59900..60418
                     /locus_tag="VUW50_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7982417.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            60426..61442
                     /locus_tag="VUW50_000462"
     CDS             60426..61442
                     /locus_tag="VUW50_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET7982418.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            61527..62783
                     /locus_tag="VUW50_000463"
     CDS             61527..62783
                     /locus_tag="VUW50_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET7982419.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQEQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            62838..64004
                     /gene="recA"
                     /locus_tag="VUW50_000464"
     CDS             62838..64004
                     /gene="recA"
                     /locus_tag="VUW50_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET7982420.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEASEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            64310..65680
                     /locus_tag="VUW50_000465"
     CDS             64310..65680
                     /locus_tag="VUW50_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET7982421.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFAFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQSSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKQPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            66056..67012
                     /gene="lytA"
                     /locus_tag="VUW50_000466"
     CDS             66056..67012
                     /gene="lytA"
                     /locus_tag="VUW50_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HET7982422.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            67390..67551
                     /locus_tag="VUW50_000467"
     CDS             67390..67551
                     /locus_tag="VUW50_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7982423.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            67538..67744
                     /locus_tag="VUW50_000468"
     CDS             67538..67744
                     /locus_tag="VUW50_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982424.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            67790..68149
                     /locus_tag="VUW50_000469"
     CDS             67790..68149
                     /locus_tag="VUW50_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982425.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMALG"
     gene            68278..68703
                     /locus_tag="VUW50_000470"
     CDS             68278..68703
                     /locus_tag="VUW50_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982426.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEGASISLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            69101..69685
                     /locus_tag="VUW50_000471"
     CDS             69101..69685
                     /locus_tag="VUW50_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982427.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKKFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGDKANMDVVLVFSKLSDGEKKQLLQARVPFVDFKG
                     NLFFPTLGLVLNANDTEVPKELIPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            69689..69886
                     /locus_tag="VUW50_000472"
     CDS             69689..69886
                     /locus_tag="VUW50_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982428.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            69876..>70338
                     /locus_tag="VUW50_000473"
     CDS             69876..>70338
                     /locus_tag="VUW50_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982429.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNSEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCG"
BASE COUNT        21399 a        12824 c        14896 g        21219 t
ORIGIN      
        1 actttttgtc tccagtcttt tcaataaata ccgtagttta ggtatacaca ttgaaattta
       61 acagatttta taactcttat aaatttttaa gaaagcaata gaagggtttt gttcaacgta
      121 taatatagag aattgaaata agatatgaac agctctatca ggaaagttaa attaatttat
      181 agaaatattt tagtatttaa ggcctactgt tatagattca atacactata tatgtgtttg
      241 tctgataaaa atttctactc tttttgattt taaataagta ttagtttaca ttatggtgtg
      301 aattgagttt gatatctctt ttgaggaagt tgccttagat ttttctgatt gtgttttatt
      361 gtatagtgta tcttgcttgt tttgaacaga attttttatg acatttgtca tattttctag
      421 tgacagaagc ttctgcctcc tctgatttta aaagactata attgtagtat aaaatggggg
      481 aagaagagat gagaaataaa atgattatcg cagtaagttt agtagtagca ggagttatga
      541 cctatctcat gttttcggga ttggatgagg atttctacca ttttccttgg aaggtctttg
      601 ctggctttgg aatcatgtct tggcttgtta gagaaggttt gaaattagtc agagatgtga
      661 aaaaggagtt tgaggaatga agagagggat catctatttc tttatctgcc tgtcactctt
      721 ggtatggtta gtggaaatgt ttactgattg gtttgatcaa gccttgcttc gccaattcat
      781 tcgtggtgct ttggggtttg gatttataat tttcgtcgtt ttccctatgg gaatggagtg
      841 gttgaaagga gagtctcatg acggtggtta aagttgagaa attgagtaag aaaataaaag
      901 acaaggaaat tttgcggaat atctcctttg aaatcaacga tggtgaatgt gttgccttaa
      961 ttggtccaaa tggtgccgga aagacaacac tcttagattg tttgcttgga gataaactgg
     1021 tcacaagcgg tcaagtatct attcaaggtt taccaatgac gagttctaag ttagactata
     1081 ctagggctta cctccctcaa gaaaatgtaa tcgttcagaa attaaaagtt aaagagttga
     1141 ttgctttctt tcaacgtatc tatccaaatc ctttgagcaa tcaggaaatt gatcaactat
     1201 tgcagtttgt caagcaacaa aaagaacagt tggcagaaaa attatcaggc ggtcaaaagc
     1261 gtctcttttc tttcgtcttg accttaattg ggcgaccaaa gattgttttt ttagatgagc
     1321 ctactgcgtc catggatacc tcaactcgtc aacgtttttg ggaaattgtc caggagttaa
     1381 aagcgcaagg agtcaccatt ctctattcgt cccattatat tgaggaagtg gaacacactg
     1441 cagaccgcat tttgctctta aataagggag agttgattcg agatacgacg cctctagcta
     1501 tgcgtagcga ggaaatagaa aagcacttta tccttcctat agcttacaag gaagtcgttg
     1561 agaagtcagt cttggttgaa aactggtcac aaaaacaaga tgctctacaa gtagtcacac
     1621 gcgaagcgga cgctttctgg gaactattag ttcaggcagg atgtaggatt caagaaattg
     1681 aagtcaataa tcgtagcttg ctggatacaa tctttgaaga aacacaaaag ggagatgact
     1741 aagatgaaac gatggatcgc actaaacaag atagaatttc tattgaccaa acgacaatta
     1801 gtctattatc tattatccgt agggatgccg acagccttct atttattctt ttcaggcatg
     1861 taccaggaca caccaggtgg accggctaat tttatgcgcg actacctcat ctccatgacg
     1921 gccttttcca tgatgtcgac cgctatcttt tcatttccag ctgttttaca taccgataaa
     1981 atcaataact ggcagaaaac attgcgccat acccctgtaa atatggtaga atattatcta
     2041 tcaaagataa caagtatgat ggttgattat ctggtctcaa tcctggttgt tttctcggtt
     2101 gggcactttg taagaggtgt ggatatgcct ctagcaagct gggttggggc tgcgattttg
     2161 cttattttgg gaagtattgc ttttgtagca cttggattga ccttgacact cttgccttct
     2221 agtcagctga tgtctgtcgt gggcaatctt ctctatctag gcttggctgt tttaggtgga
     2281 ctctggatgc ccatctcttt atttccagac tggatgcaag caatcgggaa gtgcctacca
     2341 acttatcagt tgatggagtt gctcaagacc ttcttaaacg agggtggcat caatctatca
     2401 gccacagttt atttacttgt tttttcagca gttttatttg gtttgaccat ttaccttcaa
     2461 ggtcataagg agaatgctta atgcttgaaa gactgaaaag catacattat atgttttgga
     2521 tcagtttaat ttttatgatt ttccccatcc tacctgtagt gactgggtgg ctttctgcct
     2581 ggcatttatt gattgatatt ctatttgtag tggcgtattt gggtgtttta ataactaaga
     2641 gccagcgcct atcttggcta tattggggcc tcatgctgtc ttatgtagtt gggaatactg
     2701 cctttgttgc tgttaattat atctggtttt tctttttcct atccaatctc ttaagttatc
     2761 atttcggcgt acgtagtttt aattctttac atgtccgaac ttttctcctt gctcaagtcc
     2821 ttgttgtggg gcaactttta atttttcagg aagttgaagt tgagtttcta gtctatctac
     2881 ttggaattct cacttttatc gatttaatga cttttggctt ggttcggatt cggattatgg
     2941 aggatttgaa agaagctcag gctaagcaaa atgcccagat aaatctattg cttgctgaaa
     3001 atgaacgcag tcgtatcggt caagatttgc atgatagtct ggggcatacc tttgctatgc
     3061 ttagtgttaa aactgatcta gccttgcagt tatttcaaat acaggcttat ccacaggtgg
     3121 aaaaggaatt aagagaaata cagcaaatta gcaaagaatc aatgcgtgaa gtgcgaacca
     3181 ttgtggaaaa tcttaagtct agaactttga tatccgaact agagactgtg aaaaagatgt
     3241 tagaaattgc tggaattgag gtggaaacgg ataaccaatt agatactgct agtctaactc
     3301 agaaattgga gtcaacggct tccatgattt tgcttgaatt agtgaccaac atcatcaaac
     3361 atgctaaagc gtctaaagtc tacttaaaat tagaacggac agagaaagaa ctcattctaa
     3421 cagtgagaga tgatggctgt ggctttgctt ctataaaggg ggatgacctc catacagttc
     3481 gagatcgtat tcttccgttt tcgggagaag taaaggtaat tagttggaaa caaccgacag
     3541 aagttcaggt tcgactacct tataaggaga gaaagtagat gaaagtatta gtcgcagaag
     3601 atcaaagtat gttgcgagat gctatgtgcc agttgcttgc ctttcaagca gatgtagagt
     3661 ctgtcttaca agccaagaat gggcaagaag caatccaact cttagaaaaa gagtccgtag
     3721 atattgccat tcttgatgta gagatgcctg ttaagactgg tcttgaagtc ttggagtgga
     3781 tacgagcaga aaagctcgaa acaaaggtgg ttgtagtgac gaccttcaag cgcccagggt
     3841 attttgaacg tgcggtcaag gctggagtag atgcttatgt attaaaagaa agaaacattg
     3901 cagacctcat gcaaaccttg cacaccgtcc tcgaaggagg caaggagtat tcgcctgaat
     3961 tgatggaagt ggtgatgatg catcccaatc cattaacgga gcaagaaatc gcagttttaa
     4021 agggaattgc tcagggcttg tctaaccaag aaattgcaga tcaactttat ttatcaaacg
     4081 gaaccgtccg aaactatgtc accaatattc tttcaaaact agatgcaggt aatcgaacag
     4141 aggcagctaa tatcgcgaaa gaatctggtt ggttgtgata ctatcataac tcgaaaacca
     4201 ttatgtgcta aaattgatgg gattcaatga aataatttgt aacttgagga ggcttagttt
     4261 cctccttttt gtttagtcta aggtggtagc aaggagctaa acctaggttt tctatgaaat
     4321 tttttcaatc aatcttcaaa aaacacttga aagtgtttac aattagtgat agaatggaat
     4381 aagtagaaat atgaaaacga aattttcaca tcttcacttt tgtaagtaga tatagagctt
     4441 caatcagtac ctctacttgt aaaagaatgt gtgagataga aaggacagga aaaggaatga
     4501 atgcagatga tacagtaacc atttatgatg tcgctcgtga agcaggtgtt tccatggcga
     4561 cggtcagccg tgtggtcaat ggcaataaaa atgtaaaaga gaatacccgt aaaaaagtgc
     4621 tagaggtaat tgatcgtttg gattatcgtc caaatgcagt tgcgcgtggt cttgcaagta
     4681 aaaagacaac cactgtcggt gtcgtgattc caaatattac caatggttat ttttcgagtt
     4741 tggctaaggg gattgatgat attgcagaaa tgtacaagta caatattgtc ctagctaata
     4801 gcgatgaaga taacgagaaa gaagtttctg ttgtcaatac cctcttttca aagcaggtag
     4861 atggcattat ctatatgggt tatcacttga cagataaaat tcgctcagaa ttttcgcgtt
     4921 cacgtactcc gattgttctc gcaggaactg tcgatgttga gcaccagttg ccaagtgtca
     4981 atattgacta taagcaagca acaattgatg cagtgagtta ccttgctaaa gaaaatgagc
     5041 gtattgcttt cgttagcggt ccgctagtgg atgacatcaa tggtaaggtt cgtttagttg
     5101 gctacaagga aaccttgaaa aaagcaggaa tcacttatag tgagggtttg gtatttgaat
     5161 ctaaatatag ctatgatgat ggttacgcct tagcagagcg tttgatttca tcaaatgcaa
     5221 ctgcagcagt tgtgacaggt gatgagttgg cagcaggagt cttgaacggt ttggctgata
     5281 agggtgtttc tgtgccagaa gattttgaaa ttattactag tgatgattca caaatctcac
     5341 gctttacccg tccaaacttg acaacgattg cccaacctct ttatgatctt ggtgccatta
     5401 gtatgcgtat gttgaccaag attatgcata aggaagagtt ggaagaacgt gaagttctct
     5461 tacctcatgg tttgacagaa cgtagctcaa cacgaaaacg taaatagaaa aaatcaggga
     5521 atcgagaaga tttcctgatt ttgtgctctt aaacaagaga tgttgactta atgaatacca
     5581 tagagcggac taggcgactg gttaaaggct gcgcaacaca ctgttttgag gttgtagata
     5641 gaactgacga agtcagctca aaacatgttt ttgaggttgt agatgaaact gacgaagtca
     5701 gtaaccatac atacggtaag gcgacgtgac gtggtttgca gagattttcg aagagtatta
     5761 gccttccata tagtttttca aagcctgtcc tgttagtccg gcattgaggg cgatgaggag
     5821 tttcaagcga gctttttggg cgttgagttc tttaacaaag aaaacgcctg ctttttgcaa
     5881 ctgtacgccc ccaccctggt aggcataaac aggctcggca ataccgttaa agcatcgtga
     5941 taccagagcg actggaattc ctttttgcag aaggctttct aatttttgag ccgtttcttt
     6001 gggaatatta ccagctccga aggcttggat aatcaaaccg tccaagtgtt ctaaatccag
     6061 catatcaatc agctcatctg tcataccagc ataagccgag atgataggga ctaaaccttg
     6121 tatgtgatca aggtcaaagc gaacacgagg ttcagctgtt ttgaagtaga ggatttcctg
     6181 tttcatgatg agaccgaggg ggccatgtgt tggagtctgg aaggtgctga cattagtcgt
     6241 atgtgttttg gtgacatact tggcagcgtg gatttcatcg ttcataacga ccaaaactcc
     6301 tttgtcagca gccctgtcat cgctggccac tcgtaaagca cttaggtaat tataaacacc
     6361 atcactaccg agctcattgg agctacgcat ggctcctgtt agaacgatag gcatatgggg
     6421 aacttccatg gtatcaagga aataggctgt ttcctctaaa gtatcggttc cgtgtgtgat
     6481 caccactcca tcgtagttat ctgcttcctc tttaattttc tggtagagga ccagcatatg
     6541 tttgggtttg atatggggac ttggaaggtt aaaaaagtcc aaggcgtgga cttggattcc
     6601 ttcaagtggg ttggacacat ggttcatggg attatctgaa ctcgtcacaa cagcgccaga
     6661 agcatcggcc tgcatggaaa tagttccacc cgtacgtaaa acaaggattt tcttaggcat
     6721 gatttactca ctctttctga aatgtggtat aatcattgta tcacaaaagg ggagattggg
     6781 ataggatgga agtcaaagct gttttttttg atatcgatgg aaccttggtc aacaatcgca
     6841 agagtgtttt gaaatccact aaggacgcga ttaagattgt caaagaacaa ggggtactag
     6901 ttggcgtagc gacagggcga ggaccttttt ttgttaagga attgatggac gatttggatc
     6961 tggattttgc ggtaacctat aatggccagt atatctttag taaagacaga gtcttgttca
     7021 cgagccctat ttctaagtta catttgcgcc atctaattag ctatgctaaa aaagagggca
     7081 cggagattgc cctagggacc aaggatgcca tgttgggttc taaaatcatg tcctttggtc
     7141 tgggttcttt ttctcaacga attagtcgct tcgttccctc tgttttaact cggacggtga
     7201 gtcagtcttt taatcgcatg gtcagcaaga ttgttcccca aaaggaagaa gacctgcttc
     7261 acctgatgaa tcagcctatc taccaagttt tgatgctgat gacaccagaa gaatctgaga
     7321 aggcggcagc tgattttgaa gacttgaaat tgacacgtag caatcctttt gcatcggatg
     7381 tcatcaatca gggaaattct aaattagaag gcattcgccg agttgggaaa gaatatggct
     7441 ttgacctcaa ccaagtcatg gcttttggtg actcagataa tgaccttgaa atgctggctg
     7501 gcgtcggtat gtcggtcgct atgggaaatg gtagtagcag tgtcaaggaa gctgccaagc
     7561 acattaccac tagcaatcaa caagatggga tccacaaggc cttggaacat tttggtgttt
     7621 tgtcttcaga aaaagtcttt gtcagccgtg actatcattt caataaggtc aagaccttcc
     7681 accacatgat ggatgaacga acccaagaag aacctcgagc ttgggattta gagggtgcaa
     7741 cccatagggc tggttttaaa atagaagaat tggtggagtt tgttcgagca gccagtcctt
     7801 ctgaagaaga ttttggtcaa gctgtatcgc aacttcatca ggcccttgat aaggcagcag
     7861 ataaagtagc caagaagaca cctgctcagc aagatttgat agggcaagtg gatgccttga
     7921 ttgacatgct ttacttcacc tacggcagtt ttgtcttgat gggggtggac ccagaacgta
     7981 tttttgatat tgtccatcag gccaatatgg ggaaaatttt tccagatggt aaggctcatt
     8041 ttgatccagt gacccataaa atcttaaaac cagataattg ggaagaaaaa tatgctccag
     8101 aacctgctat taaaaaggaa ctgcagcgcc agctcaaggc ttacgaacgc cataaagaga
     8161 gaaataaatc gtaaacaaaa aggagctagg ggctcctttt ctttgtgatt ataaggtttt
     8221 ttcttgttct cttacaacca gcaaatcgac cttagcatgg cggagtatgt attcagatga
     8281 agagccgacc aagaggcgtt caaaggcgtt gagaccagtt gcgccaacga ggatgaggtc
     8341 cacttcctcg gcatctggaa tagtacgtgc tagcagggtc tttggatttc ccatttcaat
     8401 gacgatatga acatctgcta ccccagcatc tttagcacgt ttttcgtact ctttcatcag
     8461 actttcagcg tcgacttgga gttcttcgta aacttcagca tcaaaggtgg atacgctttg
     8521 gagagcgcgt gtgtcaatga catgtgcgat ggtgagttta gcgtcgtttc gtagagcaga
     8581 atgaactccc ttgacaaaag ccaagtccgc ttccttagaa ccatcgattg cgaccatgat
     8641 attttggtaa cgttgtgcca taatgaaacc tcctgaaatt ttcttactat aattgtaaac
     8701 cttttcacta tagaagtaaa gcaaaaaaag tatttttcaa aagaatatta gggcttaaaa
     8761 tctatataaa tatatgataa aataaaagaa aggatagcag gaaagaagtg aaggaaggga
     8821 gaatgatgaa aaactctttt caaaaatcaa atttccttta ttatggaatt atcctagttt
     8881 cagtcttggt agaagtgatc atgatctggc aattagagag tctggtaccg cttctttatc
     8941 caagttttat tggattttta gtctttcatg tactctacca tctaatctta tttctagttg
     9001 caaaacgaag tgggcgttgg gattacttga tgacatgggg gcttttcctc atgtttaatc
     9061 ttctttatga ctcctttcta ggcttgcttt ttctaggttt atcttttggt atgtgatgtg
     9121 tctttgcaaa atacctgcta tctctacgtt cttaacctcg cctttagcaa ggtttttctt
     9181 ctatacgaaa ctagtaacac atttcagtaa acttgtcgca ttctctttct agtggtataa
     9241 tgttactatc ctgatgaatt gaggaaacaa gatgaaagaa tataacaagt ctagtaagtt
     9301 agagcatgtt gcttatgata tccgtggtcc tgtattggaa gaagccatgc ggatgcgagc
     9361 gaatggagaa aagattttac gtctgaatac aggaaatcca gcagaatttg gctttacagc
     9421 gccagacgag gtcattcatg acttgattat gaatgcgcgt gatagtgaag gctattctga
     9481 ctccaaaggg attttctcag cccgtaaggc catcatgcag tattgccagc tgaagaaatt
     9541 cccaaacgta gatattgatg atatttacct tggaaatggt gtcagtgagt tgattgtcat
     9601 gtccatgcag gggcttttgg acaatgggga tgaggtctta gtgccaatgc cagactatcc
     9661 tctatggacg gcggctgtca gcctagctgg aggaaatgcc gttcactata tctgtgatga
     9721 agctgtggaa tggtacccag atattgacga tatcaagtca aaaatcactt ccaataccaa
     9781 ggcaattgtc cttatcaatc caaataaccc aactggagcc ctttatccta aggaactctt
     9841 gctggagatt attgagattg cccgtcaaaa cgatttgatt atctttgcgg atgaaatcta
     9901 tgaccgcatg gtaatggacg gacatgtgca tacgcctgtg gcgagcttgg caccagatgt
     9961 cttctgtgtc agcatgaatg gtctgtcaaa atcccaccgc atagcaggtt tccgtgtggg
    10021 ttggatggtc ttgtctggcc ctaagactca tgttaagggc tatatcgaag ggctcaatat
    10081 gctgtccaat atgcgccttt gctctaacgt tttggcccaa caagtcgtac aaacttcctt
    10141 ggggggtcac caatcagtcg atgaattgct tcttcctggt ggacgaatct acgagcaaag
    10201 aaatttcatc tataatgcca ttcaagatat tccaggtttg tctgccgtta aacccaaggc
    10261 ggggctctat atcttcccaa aaatcgaccg caatatgtac cgtatcgatg atgatgagca
    10321 gtttgtcctt gatttcttga agcaggaaaa ggttctcttg gttcatggtc gaggctttaa
    10381 ctggcaggaa ccagaccact tccgtatcgt ttaccttcct cgtgttgatg agttagccca
    10441 aatccaagaa aagatgactc gtttcttgaa acagtatcgt agatagggct tgcattcgaa
    10501 aaagctggaa acatttgcct agagctattg acaaaagtga aagaatcaga tagaatagta
    10561 aagtatgttt agtaactgat cactttatag ccggctaaac gaatacaaaa tctatgagga
    10621 ggtattcatc gtgaaacgta cttatcaacc aagtaaactt cgtcgtgcgc gcaaacacgg
    10681 attccgtaac cgtatgtcaa ctaaaaatgg tcgtcgcgta ttggcagctc gtcgtcgtaa
    10741 aggacgcaaa gttttggctg cataatccaa acgaattcta tcaaaaatca gtaggaactc
    10801 gagtctactg atttttattt ttgtaaaaaa gttcagtaga tgcaaatgga ttcggaagcg
    10861 atgttatagt agattgaaac tagaatagta cacctctatt tctaaaacat tgttagaaat
    10921 cgatttgact gtcctgatcg atttgtcctg ttattatttc attttactat aaagttgaag
    10981 tagctggaga tggcacagcg acaatcgtct ttaaagatgg ttcagctatt acaattccag
    11041 gaaatcaatt ggtagcacaa gatccaaaag cacaagatag cactaaaccg actgctgaaa
    11101 aatcaactgt taaagcacct gctcaaagag tagatgtaaa agatataact catttaacag
    11161 atgaagaaaa agttaaggtt gctattttac aagcaaatgg ttcagcatta gacggagcga
    11221 caatcaatgt agctggagat ggtacagcaa caatcacatt cccagatggt tcagtagtga
    11281 cgattctagg aaaagataca gttcaacaat ctgcgaaagg tgaatctgta actcaagaag
    11341 ctacaccaga gtataagcta gaaaatacac caggtggaga taagggaggc aatactggaa
    11401 gctcagatgc taatgcgaat gcaggcggtg gtagccaggc gggtggatca gctcacacag
    11461 gttcacaaaa ctcagctcaa tcacaagctt ctaagcaatt agctactgaa aaagaatcag
    11521 ctaaaaatgc cattgaaaaa gcagccaagg acaagcagga tgaaatcaaa ggcgcaccgc
    11581 tttctgataa agaaaaagca gaacttttag caagagtgga agcagaaaaa caagcagctc
    11641 tcaaagagat tgaaaatgcg aaaactatgg aagatgtgaa ggaagcagaa acgattggag
    11701 tgcaagccat tgtcatggtt acagttccta agagaccagt ggctcctaat gctgctccta
    11761 agacaacaag tacaccgcaa gcaactgcag gaacaatgca agatgttacc taccagtcac
    11821 ctgctggcaa acaattacct aacacaggtt cagcatcaag tgcagcactt gctagtcttg
    11881 gtctagcggc ggcaacaagt ggttttgctt tgctaggaag aaagactaga cgtagaaaat
    11941 agaacagcta gaaaattcta ttctctactt aaagttagat tataaggggg attttgagaa
    12001 gttatcaatc ctagtgatgg gtgagaaaag tgagaaccca agataatcac atactttagc
    12061 tgaataggaa tattctatca atgtagccaa tctcttctgt ctctaactgt gggacaggag
    12121 atgggcaata tcggatagaa aagatagcag aatagctctc tattgaagag aggaggggaa
    12181 accgaaaaat taggtgcccc tcctcttttt tggtataata gaagatagaa aacgaggtta
    12241 gaagagatga tttttgatac acatacacac ttgaatgtag aagaatttgc aggtcgtgag
    12301 gcagaagaaa ttgccttggc tgatgagatg ggtgtgacac agatgaatat tgttggtttt
    12361 gataaaccga cgattgagca tgccttggag ttggtagatg agtatgagca gctctatgcg
    12421 actattggtt ggcatcctac agaagctggt acttatacag aggaagttga ggcttacttg
    12481 ttggataagt taaaacattc caaggttgtg gctttaggtg aaattggctt agactaccat
    12541 tggatgacag cgcccaaaga ggtgcaggag caggtttttc gccgtcagat tcagctatct
    12601 aaggacttgg atttgccttt tgttgtccat acccgtgatg cgctggaaga tacctatgag
    12661 attatcaaga gtgagggcgt tggtcctcgt ggtggtatca tgcattcatt ttcagggacg
    12721 cttgagtggg cagagaagtt tgtggatctt ggtatgacca tttccttctc aggagtggtg
    12781 actttcaaga aggcaactga cctccaagaa gcagctaaag agttaccttt ggacaagatg
    12841 ttggtggaaa cagatgcgcc ttacttagca cctgtaccca agcgtggtcg tgaaaataaa
    12901 acagcctata ttcgctatgt ggtcgacttt atcgctgact tgcgtggtat gacgacagaa
    12961 gagctagcgg cggcaacgac tgcaaatgca gaacgcattt ttggattgga cagcaagtaa
    13021 tgaaagagaa aatttcccaa gtcatcgtgg ttgaaggtcg tgatgatacg gtcaatctca
    13081 agcgttattt cgatgtggag acctatgaga ctcgaggttc tgccatcaat gaccaggaca
    13141 tagagcggat tcagcgtcta caccaacgtc atggagtcat tgtctttaca gacccagatt
    13201 ttaatgggga acggattcgg cgcatgatca tgacggtcat tccaacagtt cagcatgcct
    13261 ttctcaagcg agatgaagct gttcccaagt ccaagaccaa ggggcgttct ctgggaattg
    13321 agcatgccag ctatgaagac ctgaaaacgg ctctagctca ggtgacagaa caatttgaac
    13381 atgagagtca gtttgacatt agtcgtagcg acttgattcg ccttggtttt ctagcagggg
    13441 cagacagctg taagcgtaga gaatatctcg gagagactct ccgaatcggc tattctaacg
    13501 gcaagcaact cctcaaacgc ctagagttgt ttggggttac tttggcagaa gtggaagaag
    13561 ctatgaaatc ttatgagtag gaaagatgtg accgttacaa ttttttaagt ttcacagtat
    13621 ttttcgaagc aggtagaaga ggaggcgtct gatgttaatt ggtcaaaaaa ttaaagagat
    13681 tcggatagaa aaaggaatta gtcgtccaga tttttgtgga gatgagcaag aactgacagt
    13741 tcgtcaactg tcgcgaattg aaagtggagc ttcgcaaccg agtttgccca agttagacta
    13801 tattgctcgc cggctaggag ttccagttta tagccttatg ccggattttt cagctcttcc
    13861 ttctgcttat ttagaattga aataccagat tttacgtgaa ccaatctatg gtaaagaaga
    13921 ggagtacgat aagaaggaag cgtgtttgga agagatttat aaaacatact ttgataatct
    13981 tcctaaagaa gaacaattag catgtgaagt attgcaggcg tgtttggata cttctagaac
    14041 tagaaggcct gaatatgcag agttaatact tgaggaacat atgtctcaga tcatagaaaa
    14101 agaagtttat tcaacaaatg atatgttgtt gattcgtttg tttttttatc aaatgctcat
    14161 tagaaaagat cttgccaaat ttataaatca aatcgaaaag ctaatgctct ttcttttgga
    14221 acagaagaag gtaactcaaa tagagaatta ctttataatt agagatactc ttatttcagg
    14281 aatgtgttgt cttgaaaagg taggagtaac tgattgtttt aatgattatc tatcgtgttt
    14341 acaagaaatt atggataaaa ctcaagatta tcaaaagaaa cctcttgtat ttatgttttt
    14401 gtggaagcaa gcattaagag tagaaagaga ttttagttta gctgaatcat tttatcagtc
    14461 ttctaaaaca tttgcgaagc taattggaga tggatttcta gtaaagaaat tgacagagga
    14521 atggcaagag gatgtcaaaa aatatttata aacatagtga atcagtgaca aaaatgtcct
    14581 tgtcctcgta tcaaaacagt tctaaagttc gtctttagaa ctgttttttt agatataagc
    14641 taaaaataat atgaaatagt tagattttaa ggacattgat gtccttgata gttcttgaaa
    14701 aaacggatat aatatatttg aagatgttgc tagcagaaag aacttaataa gcttcttaac
    14761 atcgcagtga ctgtggtcac cttctcacta agtgaccaag taactttttg gaggaaatga
    14821 tgaaacgttt atttattttg atttcaatgg tattggtatc gctttatatg gtgataactt
    14881 ccgttgacca tcgagaagag attttatttg gtaactatcc ttctgttgat gtgacaggaa
    14941 tgatgataaa tcaaccagta gctagtcgcg aaaaggtgac agaggctttg agtcacttgg
    15001 cggtagagca taatagtctc atcgcccgtc gaatcgttga gccaaatgaa gctggagaaa
    15061 cactctttac ctatgccact tatggtgagg gagagcttcc agaaggtctg accatttcct
    15121 ccaaggagag tgcagaaacg agtgatttat tagggtctta cttgattgtg tcaggaagtt
    15181 tggatggagt gagcttacag accaccttga aagaacttgg ctatcaaggc tttgtttcga
    15241 atggagaaga tccattttcg atagtcttac tattgacggc cacccctatg gtgctactga
    15301 gtttagctat ttttctgctg acctttatga gtctgaccct gatttatcgg atcaaatccc
    15361 ttcgtcaggc agggattcgc ttaatagctg gtgagagctt gtttggagtt gctctcagac
    15421 cagtgttaga agatgtgaga cagcttatct gctcagtgct ggtatccagt attttgggat
    15481 tggggattct ctggtatcaa ggtgccttgt ttatggcaac ggtgcaactg gtcatcattg
    15541 ctcttctact ttatggattg atcttggcag ggatttctac cttactaagt gtcgtctatc
    15601 tacttggttt acaggaaaat agtctggtgg atctattgaa agggaaactc cctctcaaac
    15661 gtatgatgac attgatgatg gtggggcaac tcttagctgt attggtggtc ggatcgagtg
    15721 cgacagctct cctaccccac taccgtgaaa tgcaggaaat ggagagagct agcaataaat
    15781 ggagccagta ctcagaccgt taccgtctat cctttggttg gtctagtgca tttgccgatg
    15841 aagaaggaac gcgtaaggat aatcgtgagt ggcagacatt tactgaagaa cggttagcca
    15901 atacagactc tttttatatt atgagcaatg ttgacaattt ctcagatgga gcagaagtgg
    15961 acctagatgg taatcgtctc agtgactaca caccgtcagg gaatgttatc tatgtctcac
    16021 cacgctatct gatagaagaa aagattaccg tttcttcaga gtttatggac aagatgcaaa
    16081 acttgtctga gggagagttt gggctgatct tgcctgagag cttgcgagag cagtctgcct
    16141 actaccaagg attgtttaca gattacctgc aaaacttttc atctgaaagt gtagaagtga
    16201 cgagtcagaa acactacttc ccacaggtaa ggctagcttt tacagaaaca ggacaggaac
    16261 gtttcctcta taatgatggg tacaagacaa cacgccagta cctaaaagat ccgattattg
    16321 tagttctaac gccgcaagcg actggaacaa gacctgttgc agggatgttg tggggaacta
    16381 cggctaatag tgccttgaaa ctagatcgat atggagacag catcacagct ctaaaagagc
    16441 aaggtctgta tcacaaggtt tcttacttgg taaaaagcca gctatttttt gccaaggtac
    16501 taaatgacaa acgggtggag ttttactctc tccttattgg gacgattttg accctgtcta
    16561 cggctatctt gttatttgat tccatgaatc ttctctattt tgagcagttc agacgggagc
    16621 ttatgattaa acgtcttgct ggtatgacaa tctatgagct tcatggcaag tatttactgg
    16681 cgcaaggagg agttctcttg cttggcctag tcctatctag tattttgaca agagatggtt
    16741 tgattagcgc tctagttgta gctttgttta cgcttaacgc cctcttgatt ttagtaaggc
    16801 aggacaaaaa agaagaagct ggtagcatgg cagtattgaa aggaaaataa gatgattgat
    16861 attcaaggat tggaaaagaa atttaatgac cgcgcgattt tctctggttt gaatctcaag
    16921 ctggagaagg gcaaggttta tgccttaatc ggaaagagtg gaagcggaaa gacgacgctg
    16981 ctgaatatct tgggaaagct agaaaagata gatggaggaa gggttctcta tcaggggaaa
    17041 gatttaaaaa ccattcccac tcgtgagtat tttcgagatc agatgggcta tctctttcaa
    17101 aattttggcc tcttagaaaa ccaatcaatc aaagaaaatt tggatttggg ttttgttggt
    17161 cagaaaatct caaaagtaga acgtttggaa aggcaagtgg gggctttaga aaaagttaat
    17221 ctagggtatt tggatttaga acaaaaaatt tatactttat ctgggggaga ggcccaacga
    17281 gttgcccttg ctaagactat tttgaaaaat ccacccttga ttttggcaga tgaaccaaca
    17341 gcagctcttg atcctgaaaa ttcagaggag gttatgaatc tcttggtgga tttgaaagat
    17401 gaaaatcgaa tgatcatcat tgcgacccat aatcccctag tctggaataa ggctgatgaa
    17461 atcattgata tgaggaaact tgctcatgtg tgaaaaaatc cgtattcgca gagtatctga
    17521 ttatcctagt gccagaggtg gtttagaaga tatcctcatc atggaaaata tgaccaatca
    17581 tctccttttg gttcaaatcc gagtacatgg ctatttgctt gattttgcta gtattgaagg
    17641 gcaaaggcaa aagcattatc gtttgaaaaa tttacctcag acggttgaac tgacagtgga
    17701 tgatgtggag gaggatgtgg atttgaccct acccgaaaat cgaagttatc aagaagctga
    17761 tttttttgaa cgcatgtttc gagagaactg ttaaggccac ttttaaagat ttccaagact
    17821 atctttcttc atgaggaaag atagtttttt ggtatgattt tcattcccaa aatacaaggg
    17881 gaatgtgtta caatagtagt aacagataat agaaaagaga atagatgaga attgcagatt
    17941 atagcgtgac caaggcagtg ctggagcgtc acggttttac ctttaaaaag tccttcgggc
    18001 aaaatttcct gacggatacc aatatccttc aaaaaattgt ggatacggct gaaattgatg
    18061 accaggtcaa tgtcatcgaa atcgggccag gtattggtgc cttgacggag tttctggctg
    18121 agcgtgcagc ccaagtcatg gcttttgaga ttgaccaccg tttggtgcca attttggcag
    18181 ataccctgcg tgattttgat aatgtgactg tagttaacga agatattctc aaggttgatt
    18241 tggcgcaaca tatccaaaat tttaaaaatc ctaacctgcc aatcaaggta gtggctaatt
    18301 tgccttacta catcacgacg cctattctca tgcacttgat tgagagtggg attcctttta
    18361 gtgagtttgt ggtcatgatg cagaaagaag tagcggaccg catttcagcc cagcctaaca
    18421 ccaaggctta cggtagcttg tctatcgccg tgcagtatta catgacagcc aaggttgcct
    18481 ttatcgtgcc tcgtacggtc tttgtgccag cgccaaatgt ggattcagcc atcttgaaaa
    18541 tggtgcgccg tccagagcca gctgtagcag tggaagatga gaacttcttc tttaaggttt
    18601 ccaaggctag ttttacccat cgccgtaaga ccttgtggaa taacttgaca ggttactttg
    18661 gtaagactga agaggtcaag gacaagctga ccaaggcttt ggaccaggca ggcttgtcac
    18721 caagtgtgcg tggggaagct ctcagcttgg cagaatttgc ctgtctagca gacgcactta
    18781 aagggcaagg actctaagat gcagggacaa atcattaaag ccttggcagg tttctactat
    18841 gtggagagtg atggccaggt ttatcaaaca cgcgcgcgtg ggaatttccg taaaaaaggc
    18901 catacccctt atgttgggga ctgggtagat ttctctgccg aggaaaattc agaaggctat
    18961 atcctaaaaa ttcacgaacg gaaaaacagt ctggttcgtc cgcctattgt caatatcgat
    19021 caagctgtag taatcatgtc cgtcaaggaa cctgatttta acagcaattt gctggatcgt
    19081 ttcttggttc ttttggagca caagggcatc catcccattg tctatatttc caaaatggat
    19141 ttgttggaag ataggggaga actggatttt taccagcaga cctatggtga catcggctat
    19201 gactttgtga ccagtaaaga ggaactcctg tctttgttaa caggcaaggt tacggtcttt
    19261 atggggcaga caggtgttgg gaagtcaact cttctcaaca aaatcgcacc agacctcaat
    19321 cttgaaacag gagaaatctc agacagtctg ggtcgtgggc gccatactac tcgagctgtt
    19381 agttttttca acctcaatgg gggtaaaatc gcagacacac caggcttttc atctctggac
    19441 tatgaagtat caagggctga agacctcaat caggctttcc cagagattgc tactgtcagc
    19501 cgagattgta agtttcgtac ttgtacccat acccatgagc cgtcttgtgc cgtcaaacca
    19561 gctgttgaag agggtgttat tgcaaccttc cgttttgaca attacctgca attccttagt
    19621 gaaattgaaa atcgtagaga aacccataaa aaagtcagca aaaaaattcc aaaataagga
    19681 gaaacctatg tctcaataca agattgctcc gtcaattctg gcagcagatt atgccaactt
    19741 tgaacgtgaa atcaaacgtc tagaagcaac tggggcagaa tatgcccata tcgatatcat
    19801 ggacagtcat tttgtgccac aaatcagttt tggtgccggt gtggttgaag cacttcgtcc
    19861 acatagtaag atggtctttg actgtcactt gatggtggcg aatcctgaac accatctgga
    19921 agactttgcg cgcgcaggtg cagacatcat cagtatccat gtagaagcaa cgcctcatat
    19981 tcatggcgcc ctccaaaaaa ttcgttcact cggagttaag ccttcagtcg ttatcaatcc
    20041 tggcacatca gttgaagcca tcaagcacgt ccttcatcta gttgaccaag ttttagtcat
    20101 gacggttaat ccaggttttg gtgggcaagc ctttctgcca gaaaccatgg ataaggttcg
    20161 tgagttggtt gctcttcgtg aggaaaaagg tttgaacttt gaaatcgaag tggatggtgg
    20221 gattgatgac caaactattg cccaagctaa agaagctggt gcgaccgttt ttgtagcagg
    20281 ttcctatgtc tttaagggag atgtcaatga gcgagtacaa actctcagaa aacaactgga
    20341 ctagggttgc cgtttttgca ggcggagacc gcggtcatta tcggacagat tttgatgctt
    20401 ttgttggggt ggatcgaggc tcgctctggg tcttggaaga agacttgcct cttgctctag
    20461 cagttgggga ttttgattct gtgaccgaag aagagcgaca ggtgattcaa aaacgtgccc
    20521 agcattttgt ccaagcacga ccagaaaaag atgataccga tttggaattg gctctcttaa
    20581 ccatctttga acaaaatcct caggctcagg ttactatttt cggtgccttg ggtggccgta
    20641 ttgaccatat gttggccaat gtctttctgc ctagcaatcc taagttggca ccctatatgc
    20701 atcaaataga aattgaggat gggcaaaact tgattactta ttgtccagaa gggaccagtc
    20761 agctagaacc tcgttcagac tacgactatc tagcctttat gccagttcgg gatagccagc
    20821 tgactattct tggagccaag tatgagttga cagaggaaaa ttttttcttt aaaaaagtgt
    20881 acgcttctaa cgaatatata gatagggaag tgtcggtaac ttgcccagat ggttatgtgg
    20941 tcgtactgca tagcaaggat aggaggtagg atggaaagtt tacttattct attattaatt
    21001 gccaacctag ctggtctctt tctgatttgg caaaggcagg ataggcagga gaaacactta
    21061 agtaagagct tggaggatca ggcagatcat ttgtcagacc agttggatta ccgctttgac
    21121 caagccagac aagccagcca gttagaccaa aaagatttgg aagtggctgt cagcgaccgt
    21181 ttgcaagaag tgcggattga attgcaccaa ggcctgaccc aagtccgtca agaaatgaca
    21241 gataatctcc tccaaactag agacaagaca gaccaacgtc tccaagcctt gcaggaatca
    21301 aatgagcaac gtttggaaca aatgcgccag acggtcgagg aaaaactaga aaagaccttg
    21361 cagacacgct tacaggcttc ctttgagaca gtttctaaac aactggagtc tgtcaatcgt
    21421 ggtcttggag aaatgcagac agttgcccgt gatgtcggag ctcttaacaa ggttctctct
    21481 ggaaccaaga cgcgagggat tctgggagaa ttgcaactgg ggcaaattat tgaagacatc
    21541 atgacacctg cccagtacga acgagaatac gcaacggttg aaaactctag tgaacgagtg
    21601 gagtatgcca tcaagttacc cggacaaggc gaccaagaat acgtctatct tccaattgac
    21661 tctaagtttc cactggcaga ttattaccgc ttggaagaag cctatgagac aggtgacaag
    21721 gatgagattg aacgctgtcg taagtcactc ctagcaagcg tcaagcgctt tgccaaggat
    21781 atcaagagca agtacatagc accgcctcgg acaaccaatt ttggaatctt gtttgttccg
    21841 acagaaggtc tctactcaga aatcgtccgc aatccggtct tctttgatga tttgagacgg
    21901 gaagaacaga ttattgttgc aggaccaagt accctatcag cccttcttaa ctccctatca
    21961 gttggtttca agacccttaa tatccaaaag agtgccgacc atatcagcaa gactcttgcc
    22021 agtgtcaaga ccgagtttgg caagtttggt ggtattctgg tcaaggcaca aaaacatctc
    22081 caacatgcct ctggcaatat tgatgaatta ttaaaccgtc gtaccatagc tatcgagcgg
    22141 acgctccgtc acattgagtt gtcagaaggt gagcctgcgc ttgatctact ccattttcaa
    22201 gaaaatgagg aagaatatga agattagtca catgaaaaaa gatgagttat ttgaaggctt
    22261 ttacctaatc aaatcagctg acctgaggca aactcgagct gggaaaaact acctagcctt
    22321 taccttccaa gatgatagtg gcgagattga tgggaagctc tgggatgccc aacctcataa
    22381 cattgaggcc tttaccgcag gtaaggttgt ccacatgaaa ggacgccgag aagtttataa
    22441 caatacccct caagtcaatc aaattactct ccgcctgcct caagctggtg aacccaatga
    22501 cccagctgat ttcaaggtca agtcaccagt tgatgtcaag gaaattcgtg actacatgtc
    22561 gcaaatgatt ttcaaaattg aaaatcctgt ctggcaacgg attgtccgaa atctctacac
    22621 caagtatgat aaggaattct actcctatcc agctgccaag accaaccacc atgcctttga
    22681 aacgggcttg gcctatcata cggcgaccat ggtgcgtttg gcagacgcta ttagcgaagt
    22741 ttatcctcag ctcaataaga gcctgctcta tgcggggatt atgttgcatg acttagctaa
    22801 ggtcatcgag ttgacggggc cagaccagac agagtacaca gtgcgaggca atcttcttgg
    22861 acatatcgct ctcattgata gcgaaattac caagacagtt atggaactcg gcatcgacga
    22921 taccaaggaa gaagtcgttt tgcttcgtca tgtcatcctc agtcaccacg gcttgcttga
    22981 atatggaagt ccagtccgtc cacgtattat ggaggcagag attatccata tgattgacaa
    23041 tctggatgca agcatgatga tgatgtcaac agctctggct ttggtggata aaggagagat
    23101 gtccaataaa atcttcgcta tggataatcg ttccttctat aaaccagatt tagattaata
    23161 atttaagaaa aatgagcatt ttttaggata agaatgttcg tttttttatg tgaatatggt
    23221 ataataagta aaagacaaaa atgaatactc ttcgaaaatc tcttcaaact agggtagtat
    23281 cgccttgtcg tatgtatata tgcaggtata ttacagggtt tgtcagttct atcgacaatc
    23341 tcaaagcagt gttttgaacc accagcgacc agctttctag tttgcttttt gattttttga
    23401 ataaaaatgg aataggaaat agaaatgaaa ttaagaagaa gtgatcggat ggttgtcatt
    23461 tccaactatt tgattaataa tccttataaa ctaactagtc tcaatacttt tgctgaaaag
    23521 tatgagtctg ctaaatcatc catctcagaa gatatcgtca ttatcaaacg cgcctttgag
    23581 gaaattgaaa tcggtcatat ccagacagtg actggggctg gcggaggtgt catcttcaca
    23641 ccgtctattt cgagtcagga tgctaaggaa atggttgaag acttgcgtac caagttgtca
    23701 gaaagtgacc gtatcttgcc aggtggttat atctatctgt ctgatttgct tagcacacca
    23761 gccatcttga aaaatattgg tcgtattatt gccaaaagct ttatggacca aaaaattgac
    23821 gcggttatga ccgtagcaac taagggtgtg ccacttgcaa atgcagttgc caatgtcctc
    23881 aatgtctctt ttgtcattgt gcgccgtgac ctgaaaatta ccgaaggttc aactgttagc
    23941 gtcaactatg tttcaggttc aagtggtgac cgtatcgaga aaatgttcct ttcaaaacgt
    24001 agtcttaagg caggcagccg tgtcttgatt gtggatgact tcttgaaagg tggcggaacg
    24061 gtcaacggta tgattagtct cttgcgcgag ttcgactcag aactggcagg tgtagcggtc
    24121 tttgcggaca atgcccaaga agaacgtgaa aagcagtttg actacaagtc actcttgaag
    24181 gtaaccaata ttgatgtcaa gaaccaagcc atcgatgttg aggttggcaa tatctttgac
    24241 gaagataaat aagagataga actaaaggtt ggaacgattg tcccagcctt tctttgcaaa
    24301 cagaatagaa ggaagcttat gaaaacacca tttatcaata gagaagagtt agaagcgatt
    24361 gttgccgagt tcccgactcc ctttcacttg tatgatgaga aggggattcg tgagaaggca
    24421 agagctgtca accaagcttt ttcgtggaac aagggcttta aggaatattt tgcagttaag
    24481 gctactccaa ctccagctat tttgaaaatt ctccaagaag aaggttgtgg tgtggactgc
    24541 tctagttatg tagagctttt gatgagccat aaactggact ttctgggttc tgagattatg
    24601 ttctcttcca acaacacgcc agacaaggaa tacgcctatg cacgtgaatt gggtgcgacc
    24661 attaacttgg atgcctttga agatattgaa catctggaga gagtagcagg cattccagaa
    24721 atcatctctt gtcgttataa tcctggaggc gtttttgaac tggggacaga cattatggac
    24781 aatcctgggg aggctaagtt tggcatgacc aaggaccagc tctttgaagc ctttgctatc
    24841 ttgaaggaaa aaggagccaa gacttttggg cttcactcct tcctagcgtc caataccgtg
    24901 acccatctct attatccaga gttggctcgt cagctctttg aactggctgt tgaaatcaag
    24961 gaaaagttgg gcatttcgtt agactttatc aatctttctg gcggtattgg tgttaattat
    25021 cgtccagacc aggagccgaa cgatatcgcc ttgattggtg agggagttcg taaggtgtat
    25081 gaagaagttc ttacgtcagc aggtcttggt caggtcaaga ttttcaccga attgggtcgt
    25141 tttatgctgg cacctcacgg tgctctagtc acaagagtca ctcataagaa ggaaacctac
    25201 cgtacctatc taggtgtaga tgcctcagca gtcaacctca tgcgtccagc tatgtacgga
    25261 gcttaccatc atattagcaa cgtgacccat ccagatggac cagctgaagc ggtagatgtg
    25321 gttggttcac tctgtgaaaa caatgataaa tttgcagtca atcgcgaact gcctcataca
    25381 gaaatcggtg atttgctggt aattcatgat acaggtgccc acggattttc aatgggctac
    25441 cagtataatg ccaaattacg ttctgcggaa atcctctata ccgaagaagg taaagcccgt
    25501 caaatccgcc gtgcagagcg ccctgaggac tattttgcaa ccttatatgg cttcgatttt
    25561 gaagaataat ctgataatag attgaaaatg aaattgaaaa acagattgct ttctaaaaaa
    25621 taggcaaaaa tcttgttttt ccttcaagtc gtgatataat aaaactataa aacgttttca
    25681 aggaaggtaa cgatatgtct gaagaaacaa ttgattatgg acaagtgaca ggaatggtgc
    25741 attcgacaga aagctttggg tcagtagatg ggcccggtat tcgctttatt gtctttttgc
    25801 agggctgtca catgcgttgc cagtattgcc acaacccaga cacttgggct atggagtcca
    25861 ataagtcacg tgaacggacg gtagatgatg tcttgacaga ggccttgcgc taccgtggtt
    25921 tctggggaaa taagggtggg attacagtca gtggaggaga agctctcttg cagattgatt
    25981 tcctgattgc tctcttcacc aaggccaagg aacaaggaat ccactgtacc ttggatacct
    26041 gtgctcttcc tttccgtaat aaaccacgtt accttgagaa gtttgacaaa ctcatggctg
    26101 tcactgactt ggttcttttg gatatcaagg aaatcaacga agaacagcac aagattgtca
    26161 ctagccaaac taataaaaat atcttggctt gtgcccagta tctatcagat attggaaaac
    26221 ctgtctggat tcgccacgtg ctagttccag gattgacaga cagagatgat gacttgattg
    26281 aacttggtaa gttcgtcaag accctcaaaa atgttgataa gtttgaaatt ctaccttatc
    26341 acaccatggg tgagttcaag tggcgtgaac ttggaattcc atattccctc gaaggagtca
    26401 aaccaccaac agcagatcgc gtcaagaacg ctaaaaaact catggatacc gaaagttatc
    26461 aagattatat gaaacgtgta catggataga aaagaagcct gatggaaaca tcgggctttt
    26521 gatttgcaaa aagacttagc aaatcagcta agcctttttc ttcttatctc gaacgttgtt
    26581 ttccagcgtt gcgatttttg tgttttttct tgcttgtgat agcagttggt tgttcagggg
    26641 taacgtcttt tcgtccactt ggtttagaga aagcacttgc ttttggtggg ttcttggcta
    26701 gttcttcacg gacttttttg cgaagttttg gacgaacgat atagttgacg ataaactgtt
    26761 ggagaatcat catgaaacca ccgacaaccc agtaaagtgt gacactagct ggtgagaaga
    26821 gggagaagac gacgatcatg agtgggctca tgtaaatcat tttcttgatt tgttctcttt
    26881 gcatttcatc ttctactccg tgaagtgaaa ggagcgattg aagatagtaa aggacaccag
    26941 cacaggcaac caaaatcata cttggagaac ctagaggaat gcctaggtag cttgcttgag
    27001 caaccccttc agtatgttgg gcagcaaagt agatagcaga gaagaaaggc atttgaagga
    27061 ggatagggaa acatcctaca ccgccaaaca tgctgatacc gtgctctttt tgagcagcaa
    27121 agagagcttg ttgggcttcg agtttttctt cttgagtagt cgcttctttg agacgcgttt
    27181 ggtgtggctc aaggacgtgc ttgagggcgt tcatcttttc agagtgaagc gttgccttcc
    27241 atgattggta gataccaagt ggcaagataa tcaagcgcac gataatggtt acgatgatga
    27301 tagcgacacc aaagcctaga cctttatcag tagcgaagta cttgatagct tcagccatag
    27361 gggctccgat ggtattccaa ataaatcctg ttggctgacc tgtggtttta tcgacattga
    27421 cacaaccagt caagacaagc aacatagccg ctcccatagc tgagagtgca aaacgtttaa
    27481 tagatttcac tgtttttatt ccttctttaa aaattatacc tttctattct actgtttttt
    27541 tacaaaatat acaatagttc tggagaccta atttgcgatt ttaaagtcag agtagggagg
    27601 aaagttgctt agtttgacat ctaagtagct tacttttgaa aaaggtgtcg gtccttttcg
    27661 gatttcttgg ataaattttg ccatgatagc agatgagtct gcttgggcta agatttccac
    27721 tgtgccatcg tcgttattcc atactcgacc tgtgatgcca ccaatttcaa gtgccaagct
    27781 gtaaacaccc caacgaaagc cgactccctg taccctacct tgggcaatca ttctaacctt
    27841 ttgcatacca aacccctttg attgtgttat aatatttcta tgactattat aacctcaaaa
    27901 gccaattctg tggtaaaaaa tgccaagaaa ttacgccaaa aaaaataccg caagtctgcc
    27961 tacttgattg aaggctggca cttgtttgaa gaagctgtcc aagctggagt gacgattgag
    28021 aaaatctttg ccctagaaaa ttatcgagat cagttagcta ctttttcgca aactgtctgg
    28081 gtgtcagagg atattttgct agatttggcg gattctcaga ctccacaggg aattgttgcc
    28141 gtggttcaaa aagaagaagt aggacaagct gatttgagtc agggcaagtt cttgtttttg
    28201 gaagatgtgc aagatcctgg taatgtagga actatcattc gaactgcgga tgcagcaggt
    28261 tttacaggag tgattgtttc agataagtcg gcagacatct acagtctcaa aaccctacgt
    28321 tccatgcaag gtagtcattt ccatctgccc atttaccgga tgactagtca agcgcttctt
    28381 gacgaaacta aaaaggtagc tatcccagtg ctagcaacaa ccctatctaa agattctgtt
    28441 gattacagag aactgccttc tatagaaaat tttgtactag ttatgggaaa tgagggtcaa
    28501 ggaattagtc cccttatggc tgaaagtgca gaccagttgg tccatattag catgaagggg
    28561 caggccgaga gtttgaatgt tgcggttgca gccggtattt taatcttcta tttaagctaa
    28621 ttttaacttt ttttgttata atcaaggaaa gatgttcaca gaaaaggaga aatggatgaa
    28681 tcacactatt atacatgacc gtgcaggtct caatcaattt tacgctaagg tttatgcctt
    28741 tgttggtctg ggaatcggac tatctgcttt ggtatcaggc cttatgttga cggtctttca
    28801 gtctcagttg gtttactttt tgatgcaggg gcgtctctgg ttgaccattg ctacttttgc
    28861 ggagttggct ttggttttcg ttgccagtag catggcttca agaaatagtc catctgctct
    28921 tccagtattt ttactttact ccgttttaaa cggctttacc cttagttttg tggtggcttt
    28981 ctatactccg ggtacagttt tatctgcctt tgtatcgagc gcccttctct tctttgtcat
    29041 ggcggcagtt ggtatcttta ccaagaaaga cttgagtggg attggtcgag caatgatggc
    29101 agctctcatt ggtttactga ttgctatggt agtcaatatt ttcttggcta gtggcttctt
    29161 tgattatatg attagcgtag ccatggtttt ggttttttca gggttgattg cttgggataa
    29221 ccaaaagatt cgcctcgcct atgaacaatc acaaggtcgt gtagcgacag gctgggtggt
    29281 gtcaatggct ctcagcatct atcttgattt tatcaatctt ttcctaagta ttctacgtat
    29341 ctttggtcgc aatgactaaa aggtctatga aatcagtgtt ttgagtgaca ctgatttttt
    29401 taaattttct cttttctttt cttggaaata ggtgtataat acgtttatta aatttttgag
    29461 gagttgtcta tgaagaaaag ttttatccat caacaagaag aaatttcctt tgtcaaaaat
    29521 acttttaccc agtatttgaa agataagcta gaagttgtcg aagttcaagg tcctatcttg
    29581 agtaaggtcg gtgacggaat gcaggacaac ctgtctggtg tggaaaatcc agtatcggtc
    29641 aaggttctcc aaatccctga tgctacttat gaagtggtgc actcacttgc taaatggaaa
    29701 cgccacacct tggctcgttt tggctttggt gaaggagagg gtctctttgt ccacatgaaa
    29761 gcccttcgtc cagatgagga ttccttggat gcaacccact ctgtttatgt tgaccagtgg
    29821 gactgggaga aggttatccc aaatggtaag cgtaacatcg tttatctaaa agaaacagtt
    29881 gagaagattt ataaggctat tcgcctgact gagctagctg ttgaagcccg ctatgacatc
    29941 gagtctatct tgccaaaaca aatcaccttt atccatacag aagaattggt agaacgctac
    30001 ccagacttga cacctaaaga acgtgaaaat gcgatttgta aagaatttgg agccgtcttt
    30061 ttgattggta tcggtggcga gttgccagat ggtaaaccgc acgatggacg tgcaccagac
    30121 tatgatgact ggacaagcga gtctgagaat ggctacaagg gtctaaatgg tgatattctt
    30181 gtctggaatg agtctttagg tggagccttt gagttgtctt ctatgggaat tcgtgtagat
    30241 gaagaaactc ttagacgtca ggtggaaatt acaggtgatg aagaccgctt ggaattggaa
    30301 tggcacaagt ctttgttgaa tggcctattc ccattgacaa tcggtggagg aattggacaa
    30361 tctcgtatgg ccatgttcct acttcgcaag agacacatcg gagaagtgca aacaagtgtt
    30421 tggcctcaag aagtccgcga tacttacgaa aatattttgt agagaatcga accgcaaggt
    30481 tcggttttct ttctcttttt gtctataatt tggtataata aacagtatga aaatcgtatc
    30541 aggaatctat gggggacgtc ccctcaagac actagaaggc aagacaacaa gacctacttc
    30601 ggataaggtt aggggagcca tttttaacat gattggtccc tactttgaag ggggacgagt
    30661 cttggacctt tatgcaggta gtggtggttt atctatcgaa gcagtatcgc gtggcatgtc
    30721 cagtgctgtt ttggtggagc gagaccgtaa ggctcagacc atcgtggctg aaaatatcca
    30781 gatgaccaag gaagttggaa aatttcaact cctcaagatg gatgcagaga gggcattgga
    30841 acaggtatct ggggaatttg acctcgtttt cttagaccct ccctatgcca aggaacaaat
    30901 cgtagcagat attgaaaaaa tggctaagag agagcttttt tctgaagatg ttatggttgt
    30961 gtgcgagacg gataaggccg ttgaacttcc agaagagatt gcctgtctgg gtatctggaa
    31021 ggaaaagatt tatggaatta gtaaggtgac agtctatgtc agataagatt ggcttattca
    31081 caggctcatt tgatccgatg acaaatgggc atctggatat cattgaacgg gcgagcagac
    31141 tttttgataa gctttatgtg ggtatttttt ttaatcccca caaacaagga tttctccctc
    31201 ttgaaaatcg taaacggggg ctagaaaagg ctttgggaca tttggaaaat gttgaagtcg
    31261 tggcttctca tgatgaattg gtggtcgatg ttgcaaaaag actgggggct actttcctag
    31321 tacgggggtt gaggaatgcg gcggatttgc aatatgaagc tagttttgat tactacaatc
    31381 atcagctgtc ttctgatata gagactattt atttacatag ccggcctgaa catctctata
    31441 tcagttcatc aggtgttcga gagcttttga agtttggtca ggatattgcc tgccatgttc
    31501 ccgagagtat tttggaggaa ataagaaatg aaaaaaaaga ttagatggcc cttatacgtc
    31561 attgcggcct tgattgtgac tttcttggca tttgtagtgc ccttgcctta ttatatagag
    31621 gttccaggtg gttcggaaga tattcgccaa gtccttaaag taaatgacac agaagataag
    31681 gaagctggtg cctatcaatt cgttacggtt ggtgttcaac atgccacttt agctcatatg
    31741 atttatgctt ggttgacacc ttttacagat attcgtagtg ctcaggagac tacaggtggt
    31801 tcttccgatg ttgaatttat gcgaatcaat caattctaca tgcaaacatc gcaaaatatg
    31861 gccaagtatc aaggactaaa aacagctggt aaggatatcg aactcaagta ctttggagtt
    31921 tatgttttga atgtgacgga taattcaacc tttaaaggga ttctcaatat ctctgataca
    31981 gtcacagcag tcaatgatca gacctttgat agttccaaag acttgattga ttacgtcagt
    32041 tctcaaaaat taggggattc cgtcaaggtc acctatgaag aggatgggca aaccaagtct
    32101 gcagaaggaa aaatcatcac cttggaaaat ggcaaaaatg gaattggaat cggcttgatt
    32161 gaccgtacag aggtaatcag caatgtccca attagctttt caacagctgg tattggcggt
    32221 ccaagtgctg gtctcatgtt tagtctagct atctatactc aaatagctca cccagatctt
    32281 cgtaatggtc gtattgttgc cggtacaggt accattgacc gcgatggtaa tgtgggagac
    32341 attggaggta ttgataagaa ggttgtagct tcggctaggg caggtgctgc tattttcttt
    32401 gctcctgata accctgttag cgaagaagaa caaaaggcgc atccggacgc gaaaaacaac
    32461 taccaaacag ccctagaagc ggctaaaaca atcaagacgg atatgaaaat cgtgcccgtt
    32521 aaaaccctac aagatgcgat tgattacttg aaaaacaatc cctaattttt gagtgaagtt
    32581 tccaaactag cgcacaaaca gagaagatgg tataatagtc aaatggttca attattattc
    32641 actctaagta gtcatatgtt ctttatttat gtgagttttt accttttaaa ggatcttgtt
    32701 agatgggaga aggttttaaa agtgacagat gataatacaa gaaaagttcg tttattagta
    32761 gcctttttta gcattgtcat aggctacatc ctgagttctt tctttattag cctgtatcat
    32821 ttgtggcaag aagcgcttag aggattatta tgaaatcaag agtaaaggaa acgagtatgg
    32881 ataaaattgt ggttcaaggt ggcgataatc gtctggtagg aagcgtgacg atcgagggag
    32941 caaaaaatgc agtcttaccc ttgttggcag cgactattct agcaagtgaa ggaaagaccg
    33001 tcttgcagaa tgttccgatt ttgtcggatg tctttattat gaatcaggta gttggtggtt
    33061 tgaatgccaa ggttgacttt gatgaggaag ctcatcttgt caaggtggat gctactggcg
    33121 acatcactga ggaagcccct tacaagtatg tcagcaagat gcgcgcctcc atcgttgtat
    33181 tagggccaat ccttgcccgt gtgggtcatg ccaaggtatc catgccaggt ggttgtacga
    33241 ttggtagccg tcctattgat cttcatttga aaggtctgga agctatgggg gttaaaatta
    33301 gtcagacagc tggttacatc gaagccaagg cagaacgctt gcatggcgct catatctata
    33361 tggactttcc aagtgttggt gcaacgcaga acttgatgat ggcagcgact ctggctgatg
    33421 gggtgacagt gattgagaat gctgcgcgtg agcctgagat tgttgactta gccattctcc
    33481 ttaatgaaat gggagccaag gtcaaaggtg ctggtacaga gactataacc attactggtg
    33541 ttgagaaact tcatggtacg actcacaatg tagtccaaga ccgtatcgaa gcaggaacct
    33601 ttatggtagc tgctgccatg actggtggtg atgtcttgat tcgagacgct gtctgggagc
    33661 acaaccgtcc cttgattgcc aagttacttg aaatgggtgt tgaagtaatt gaagaagacg
    33721 aaggaattcg tgttcgttct caactagaaa atctaaaagc tgttcatgtg aaaaccttgc
    33781 cccacccagg atttccaaca gatatgcagg ctcaatttac agccttgatg acagttgcaa
    33841 aaggcgaatc aaccatggtg gagacagttt tcgaaaatcg tttccaacac ctagaagaga
    33901 tgcgccgcat gggcttgcat tctgagatta tccgtgatac agctcgtatt gttggtggac
    33961 agcctttgca gggagcagaa gttctttcaa ctgaccttcg tgccagtgca gccttgattt
    34021 tgacaggttt ggtagcacag ggagaaactg tggtcggtaa attggttcac ttggatagag
    34081 gttactacgg tttccatgag aagttggcgc agctaggtgc taagattcag cggattgagg
    34141 caagtgatga agatgaataa gaaatcaagc tacgtagtca agcgtttact tttagtcatc
    34201 atagtactga ttttaggtac tctggctcta ggaatcggtt taatggtagg ttatggaatc
    34261 ttgggcaagg gtcaagatcc atgggctatc ctgtctccag caaaatggca ggaattgatt
    34321 cataaattta caggaaatta ggctggagaa ccagcctttt tctaaagata aggagaaata
    34381 tgaacaaaaa aacaagacag acactaatcg gactgctagt gttattgctt ttgtctacag
    34441 ggagctatta tatcaagcag atgccgtcgg cacctaatag tcccaaaacc aatcttagtc
    34501 agaaaaaaca agcgtctgaa gctcctagtc aagcattggc agagagtgtc ttaacagacg
    34561 cagtcaagag tcaaataaag ggtagtctgg agtggaatgg ctcaggtgct tttatcgtca
    34621 atggtaataa aacaaatcta gatgccaagg tttcaagtaa gccctacgct gacaataaaa
    34681 caaagacagt gggcaaggaa actgttccaa ccgtagctaa tgccctcttg tctaaggcca
    34741 ctcgtcagta caagaatcgt aaagaaactg ggaatggttc aacttcttgg actcctccag
    34801 gttggcatca ggtcaagaat ctaaagggct cttataccca tgcagtcgat agaggtcatt
    34861 tgttaggcta tgccttaatc ggtggtttgg atggttttga tgcctcaaca agcaatccta
    34921 aaaacattgc tgttcagaca gcctgggcaa atcaggcaca agccgagtat tcgactggtc
    34981 aaaactacta tgaaagcaag gtgcgtaaag ccttggacca aaacaagcgt gtccgttacc
    35041 gtgtaaccct ttactacgct tcaaacgagg atttagttcc ctcagcttca cagattgaag
    35101 ccaagtcttc ggatggagaa ttggaattca atgttctagt tcccaatgtt caaaagggac
    35161 ttcaactgga ttaccgaact ggagaagtaa ctgtaactca gtaaaagata agcctaaact
    35221 cctatgtcac ttatggatgt aggagttctt tttactagtt taagcagggc tagaacaggt
    35281 actaagaaaa aatagcaact tctaaaacta acttccagtt ttgggagaga gatggaagtt
    35341 actttgagaa gttacggtaa gaaaaaataa tatgtattgg attaaatttt gtgatataat
    35401 aaaggataag atactatttt aggagaagaa ctatggaaga cccaagtagt cagaatttgt
    35461 tgctacaatt tgttttattg tttatcttga cggtattaaa tgcctttttc tcagccacag
    35521 aaatggccat ggtttctctt aatcgtgccc gagttgaaca aaaggcggaa gaaggagaca
    35581 gacgctacat ccgcctgctg aaggtactag aaaatcctaa ccacttttta tcaaccattc
    35641 aggtaggaat caccctgatt acaatcttat caggggcgag tttaactaat actctaggac
    35701 gtgtgattgc ctcttggctt gggaatggcg aaacagcaca agccgtggca acttttctat
    35761 ccttggcatt tttgacctat atttccatcg tttttgggga attatatcct aagagaatcg
    35821 ctcttaatct aaaggatgcc ttggcaattc gtacagcgcc gattatcatt gggatcggaa
    35881 aactagtcag tccttttgtt tggcttttag ctgcttcgac caatttcttg agccatttga
    35941 ctcctatgtc attcgatgat gcggatgaaa agatgacccg tgatgaaatt gcctatatgc
    36001 tgacaaatag tgaagaaaca ttggatgctg atgagattga gatgctacaa ggtgtctttt
    36061 cgctcgatga actgatggca cgagaggtta tggtgcctcg aacggatgcc tttatggtgg
    36121 atattcagga tgatagtcaa gccattatcc aaagtatttt aaaacaaaat tattctcgta
    36181 tcccggttta tgatggggat aaggacaatg taattggaat cattcacacc aagagtctcc
    36241 ttaaggcagg ctttgtggac ggttttgaca atattgtttg gaagagaatt ttacaagatc
    36301 cactttttgt acctgaaact atttttgtgg atgacttgct aaaagaactg cgaaataccc
    36361 aaagacaaat ggcaatcttg ctggatgaat acggtggtat ggctggtttg gtgactttgg
    36421 aagacctctt agaggaaatt gttggtgaaa ttgatgatga aacggacaaa gcagcaatag
    36481 atgttcatca gattggcgag gatacctaca ttgttcaagg aactatgacc ctcaacgatt
    36541 tcaataatta ctttaatgtt gaactggaaa gtgatgacgt tgatactatc gctggttatt
    36601 atttgacagg agtgggaacc attccaacga ctgagaaact cagctatgaa ttggtcagcc
    36661 aaaacaaaca gtttatctta accaatgata aagtgaagaa tggacgtgtt accaaggtaa
    36721 aagtccaaat cacagaagtc gaaatagaag aagaaacaga ataaactaga ggcttttgtc
    36781 accaggcgtg acgaagcttt tttcaattga tttcaaaagg gaattatgcc atggaaagaa
    36841 ctctgccaca aactcgctcc aaaagttttt aaagttatac gtatttattc gagggaaaat
    36901 aagaaaagcc cttcaaattg ggctttctgt tcatttgaaa cctgataaat caagttatag
    36961 aaggcggtag acggatttga accgacgatc aagcttttgc agagccgtgc cttaccactt
    37021 ggctataccg ccttaacgtt tattattata ccttgaaaat gattttcagt caatagtttt
    37081 ttcagttttt tgtaccatag cgtgattcga atgtgatgcc aagttttcta tgggaaaaaa
    37141 tttaccaaaa acctactgaa accccttgaa aaatctagct aaatagggta taatatgggt
    37201 aatcatttgt cgtaggtttt gtctgaaata ttgtccagac aaggctcaca gcagttaaat
    37261 cttctgaaaa agtcagattt agctgctctt tttgtgcttt ttttcaagat tttgagcatt
    37321 tgtaacagag gcttaaagat tctgaaaatt cgtcaagagg acacggtgat aaggggttta
    37381 caaccatatg acgattagaa aagcctgatt gacaaggctt ggaacttatt tacaaaggag
    37441 aatcatcttg gcaggacatg acgttcaata cgggaaacat cgtacccgtc gtagtttttc
    37501 aagaatcaaa gaagttcttg acttaccaaa tttgattgaa attcaaactg actcattcaa
    37561 agctttccta gaccacggtc ttaaggaagt gtttgaagat gtattgccaa tttcaaactt
    37621 cacagacaca atggagttgg aatttgttgg atatgaaatc aaggaaccaa aatacacgct
    37681 agaagaagct cgtatccacg atgctagcta ctcagcacca atttttgtaa ccttccgttt
    37741 gatcaataaa gaaacaggcg aaatcaagac ccaagaagtt ttctttggtg atttcccaat
    37801 catgacagaa atgggtactt tcatcatcaa tggtggtgaa cgtattatcg tttctcagtt
    37861 ggtccgctca ccaggtgttt actttaacga caaagtagac aaaaatggta aggtgggcta
    37921 tggttcaact gttatcccta accgtggagc ttggttggaa cttgaaagcg actcaaaaga
    37981 tatcacctac actcgtatcg accgtactcg taagattcca tttacaacct tggttcgtgc
    38041 tcttggtttc tcaggtgatg atgaaatctt tgatatcttt ggtgacagcg aattggttcg
    38101 caacactgtt gaaaaagata tccacaagaa tccaatggac tctcgtacag acgaagcctt
    38161 gaaagaaatt tacgaacgcc ttcgtccagg tgagcctaag acggctgaaa gctcacgtag
    38221 cttgcttgta gctcgcttct ttgacccacg tcgttatgac ttggcagcag ttggtcgtta
    38281 caaaatcaat aaaaaactca atgttaaaac acgtttgctc aaccaaacca ttgcagagcc
    38341 attggtagac cctgaaactg gagaaatctt ggtagaagct ggtacgatta tgactcgtag
    38401 cgtgattgaa agcattgaaa gccatttgga tggcgacttg aacaagattg tctacatccc
    38461 aaacgatgca gccgttgtga ctgagcctgt tgttcttcaa aaattcaagg ttattgctcc
    38521 aactgatcca gatcgcgtcg taacgatcat tggtaatgct aacccagatg acaaggttcg
    38581 tacggtgact cctgcagata tccttgctga gatgagctac ttcctcaact tggctgaagg
    38641 acttggccgt gtagatgata tcgaccacct tggaaatcgt cgtatccgtg cggttggtga
    38701 attgcttgcc aaccaagtac gtttgggact ttctcgtatg gaacgtaatg tccgtgaacg
    38761 tatgtctgtt caggacaatg aagtcttgac accacaacaa attatcaata tccgtcctgt
    38821 aacagctgcg gttaaagaat tctttggttc atcacagttg tcacagttca tggaccaaca
    38881 caacccgctt tctgagttgt ctcacaaacg ccgtttgtca gccttaggac ctggtggttt
    38941 gactcgtgac cgtgccggat atgaagtacg tgacgtgcac tacactcact atggtcgtat
    39001 gtgtccaatc gagacacctg aaggacctaa catcggtttg atcaataact tgtcatctta
    39061 cggacacttg aacaaatatg gttttgttca aacaccatac cgtaaggttg accgtgaaac
    39121 aggtgttgtc acgaacgaaa ttgtttggtt gacagctgat gaagaagatg aatatactgt
    39181 agctcaggct aactctcgtc tgaatgaaga tggaaccttt gctgagaaga ttgtcatggg
    39241 acgtcaccaa ggggtcaacc aagagtatcc agctaatatt gttgactaca tggacgtttc
    39301 accaaaacag gtagttgccg ttgcgacagc atgtattcct ttcttggaaa acgatgactc
    39361 caaccgtgcc ctcatgggag ccaatatgca acgtcaggct gtgccattga ttaatcctca
    39421 ggcaccttac gttggtactg gtatggaata ccaagcagcc cacgattctg gtgcggctgt
    39481 gattgctcag tatgatggta aagttactta cgcagatgct gacaaggtag aagttcgtcg
    39541 tgaagatggt tcattggatg tttaccacat ccaaaaattc cgtcgttcaa actcaggtac
    39601 tgcttacaac caacgcactc tcgtaaaagt tggtgatgtc gttgaaaaag gcgatttcat
    39661 cgctgacgga ccttctatgg aaaatggaga aatggcgctt ggacaaaacc caatcgttgc
    39721 ctacatgact tgggaaggtt acaacttcga ggatgccgtt atcatgagcg aacgcttggt
    39781 gaaggacgat gtctacacat ctgttcacct tgaagaatac gaatcagaaa cgcgcgatac
    39841 aaagcttggg cctgaagaaa tcactcgcga aattccaaac gttggtgaag atgccctcaa
    39901 agaccttgac gaaatgggga ttatccgtat tggtgctgag gttaaagaag gtgatattct
    39961 tgtaggtaaa gtaacaccta agggtgagaa agatctttca gctgaagaac gtctcttgca
    40021 cgctatcttt ggagacaagt ctcgtgaagt gcgtgatact tctcttcgtg taccacacgg
    40081 tgccgatggt gtcgttcgtg atgttaagat ctttacacgt gtaaatggag atgagttgca
    40141 atcaggtgtt aacatgttgg ttcgtgttta catcgctcaa aaacgtaaga ttaaggtcgg
    40201 agataaaatg gccggacgtc acggaaacaa aggggttgtc tctcgtatcg ttcctgtaga
    40261 agacatgcct taccttccag acggaactcc agtcgacatc atgttgaacc cacttggggt
    40321 gccatcacgt atgaatatcg gtcaggttat ggagcttcac cttggtatgg cagctcgtac
    40381 tcttggtatt cacattgcga caccagtctt tgatggagca agttctgaag atctttggtc
    40441 aactgttaaa gaagcaggta tggatagcga tgccaagaca atcctttacg atggacgtac
    40501 aggtgaacca tttgataacc gtgtttctgt tggagtcatg tacatgatca aactccacca
    40561 catggttgac gataaattgc acgcgcgttc agtcggacct tactcaactg ttacccaaca
    40621 accactcgga ggtaaagctc agtttggtgg acaacgtttc ggtgagatgg aggtttgggc
    40681 tcttgaagcc tacggtgcgt caaatgtcct tcaagaaatc ttgacttaca agtcggacga
    40741 tatcaacgga cgtttgaaag cctatgaagc tattacaaaa ggcaaaccaa ttccaaaacc
    40801 aggtgttcca gaatccttcc gagttcttgt caaagaattg caatctcttg gtcttgacat
    40861 gcgtgtccta gacgaagatg accaagaagt ggaacttcgc gacttggatg aaggaatgga
    40921 cgaagatgtc atccacgtag atgaccttga aaaagcccgc gaaaaagcag cccaagaggc
    40981 taaagcagcc tttgaagctg aagaagctga gaaagcaaca aaagcggaag caacagaaga
    41041 agctgctgaa caagaataag cagttcactt agaatagaaa gggaagaaat agtggttgat
    41101 gtaaatcgtt ttaaaagtat gcaaatcacc ctagcttctc caagtaaagt ccgttcatgg
    41161 tcttatggag aagtcaaaaa acctgaaaca atcaattacc gtaccttgaa accagaacgt
    41221 gaaggactct ttgatgaagt gatctttggt cctacaaaag actgggaatg tgcttgtggt
    41281 aagtacaaac gcattcgtta cagaggaatt gtttgtgacc gctgtggggt tgaagtaacg
    41341 cgtacgaaag ttcgtcgtga gcgtatggga catatcgaat tgaaagctcc tgtatctcac
    41401 atctggtact tcaaggggat tccaagccgt atgggcttga cccttgatat gagccctcgt
    41461 gccctcgagg aagttatcta ttttgcggct tatgtggtga ttgatcctaa ggatacacca
    41521 cttgagcaca agtctatcat gacagagcgc gaataccgag agcgcttgcg cgaatatggt
    41581 tatggttcat ttgttgccaa gatgggtgcg gaagccatcc aagacctttt gaagcaagta
    41641 gatcttgaaa aagaaattgc tgaactcaaa gaagaattga aaacagctac tggacaaaaa
    41701 cgtgtcaaag ccatccgtcg tttggatgtt ttggatgcct tttacaagtc tggaaacaaa
    41761 cctgaatgga tgattcttaa catccttccg gttatcccac cagatcttcg tccaatgttg
    41821 cagttggatg gtggccgttt tgcctcatct gacttgaatg acctttaccg ccgtgttatc
    41881 aaccgtaaca accgtttggc tcgtttgctt gagttaaatg caccaggtat catcgttcaa
    41941 aatgagaagc gtatgcttca agaagcagtt gacgctttga ttgacaatgg tcgtcgtggt
    42001 cgtccaatca caggaccagg tagccgtcca ttgaaatcat tgagccacat gcttaaaggt
    42061 aaacaaggac gcttccgtca aaacttgctc ggtaaacgtg ttgacttctc aggacgttcc
    42121 gttatcgccg ttggtccaac tcttaagatg taccaatgtg gtgtgccacg tgaaatggcg
    42181 attgaactct ttaaaccatt tgttatgcgt gaaatcgttg cccgtgatat cgtgcaaaac
    42241 gtcaaagcag ctaaacgctt ggtggaacgc ggagatgagc gtatctggga tatccttgaa
    42301 gaagtgatta aagaacaccc agtgcttttg aaccgcgcac cgacccttca ccgtttgggt
    42361 atccaagcct tcgagccagt cttaattgat ggtaaggctc ttcgcttgca cccacttgtc
    42421 tgtgaagcct acaatgctga ctttgacggg gaccaaatgg ccatccacgt accactttca
    42481 gaagaagcac aagcagaagc tcgtatcctc atgctagctg ctgagcacat cttgaacccg
    42541 aaagatggga aaccggtagt tactccatct caggacatgg ttttgggtaa ctactacttg
    42601 accatggaag aagctggtcg cgaaggtgaa ggaatggtct tcaaagaccg tgacgaagcg
    42661 gttatggctt accgcaatgg ttatgttcac ctccactcac gtgttggtat cgcaacagac
    42721 agcctcaaca agccttggac agaagagcaa agacataagg tcttgcttac aacagttggt
    42781 aaaattctct tcaacgatat catgccagag gggctaccat acttgcaaga accaaacaat
    42841 gccaacttga cagaaggtgt tccagctaaa tacttcttgc cacttggtgg agatatcaag
    42901 gaagctatca gcaatcttga gctcaaccct ccattcaaga agaaaaacct tggaaatatc
    42961 atcgctgaaa tcttcaaacg tttccgtacg acagaaactt ctgccctact tgaccgcatg
    43021 aagaacctcg gttaccacca ctcaactctt gcaggattga cagtgggtat tgccgatatc
    43081 ccagtcgttg atgacaagac tgaaatcatt gaagaatcac acaaacgtgt cgaacaaatc
    43141 accaaacaat tccgtcgtgg tatgatcaca gacgacgagc gttacaatgc tgttacagct
    43201 gaatggcgtg ctgcccgtga aaaacttgag aaacgcttga ttgctaacca agatcctaag
    43261 aacccaatcg ttatgatgat ggactctgga gcccgtggta acatctcaaa cttctcacag
    43321 cttgccggta tgcgtggtct gatggctgct ccgaatggac gtatcatgga attgccaatc
    43381 ctttcaaact tccgcgaagg tttgtcggta ttggaaatgt tcttctcaac tcacggtgct
    43441 cgtaaaggta tgaccgatac ggcccttaag acagccgact caggttactt gactcgtcgt
    43501 ttggtcgacg ttgcccaaga cgttatcatc cgtgaggacg actgtggaac tgaccgtggt
    43561 ctcttgatcc gttctatcgc agaaggaaaa gagatgatcg agtctctcga agagcgtctc
    43621 aacggtcgtt acactaagaa aactgttaaa catccagaaa ctggtgcagt gattattggt
    43681 ccaaatgaat tgattacaga agacaaggcg cgtgaaattg tcaatgctgg tgtggaagaa
    43741 gtgactatcc gttctgtatt tacatgtaac actcgtcacg gtgtctgccg tcactgttac
    43801 ggtatcaact tggcgactgg tgatgcggtt gaagttggtg aagcagttgg tacaattgct
    43861 gcccaatcta tcggggaacc tggtacacag cttacaatgc gtaccttcca cacaggtggg
    43921 gttgcctcaa ataccgatat cactcagggt cttcctcgtg tccaagaaat ctttgaagcc
    43981 cgtaatccta aaggggaagc ggttatcaca gaggttaaag gacaagttac tgctatcgaa
    44041 gaagatgcat caactcgtac caagaaagtc tttgttaagg gtgaaactgg cgaaggtgaa
    44101 tatgtcgttc catttacagc tcgtatgcgt gtcgaagttg ggggccaagt agcgcgtggt
    44161 gctgctctga cagaaggttc tatccaacca aaacgtctcc ttgcagttcg tgatgtcttg
    44221 tcagttgaaa cgtaccttct cggtgaagta caaaaagttt accgtagcca aggggtagaa
    44281 atcggtgaca aacacatcga ggtaatggtt cgtcaaatga tccgtaaagt ccgtgtcatg
    44341 gatccaggtg atacagatct tctcatgggt accctcatgg atatcaatga ctttacagat
    44401 gccaacaaag atgtccttat cgcaggtgga gttccagcga caggtcgccc agtccttatg
    44461 ggaattacca aagcctcact tgaaacaaac agtttcttgt cagcagcttc cttccaagaa
    44521 acaactcgtg tccttactga cgcagctatc cgtggtaaga aagaccatct ccttggactt
    44581 aaagaaaatg ttatcatcgg taagatcatc ccagctggta ctggtatggc ccgttaccgt
    44641 aaccttgaac cacatgctgt caacgaagaa gaatacctta atcctacagt agaggaagaa
    44701 ggaaatgaag aaacaacaga agtagttgtg gatactgccg ttgaaactgt ggaagaaaca
    44761 gtagaataag agagaatgag agaaccctcg ggttctctca ttctctcttc tgaaaacttt
    44821 ctccattttt ccacgaaatg tggtaaaata aaagaaagaa gctgacaaag gagacgggta
    44881 tggaacaaac attctttatc atcaaaccag atggtgtaaa aagagggcta gtgggtgaag
    44941 tgttaaagcg catcgaacaa cgtggattta caatcgaaaa attggagttt cgttcacagg
    45001 tttcagaaga gttgattgac cagcactatc aggacttggt tggtcagagt ttttacctac
    45061 cgattcgtga attcatgact tcaggtccag ttcttgtggg tgtcatttct ggtcccaaag
    45121 taatcgaaac ttggcggacc atgatgggtg caactcgtcc agaagaagct ttaccaggca
    45181 ctattcgagg tgactttgca aaagctgctg gagaaaatga ggttatccaa aatgttgtac
    45241 atggttcaga ttcagaagag tcagctaagc gagaaattgc tctttggttt taagagtgga
    45301 ttggctcaat caattggtta aaagctcatt tgaatagaaa gtatagtcaa ttagtttaag
    45361 acatgacgca tgatatcaaa ctttttagtt tttgatatgg tgcgtttttt tgattaagtg
    45421 actattagtt cgtctcgctc cgttaggtgc gagacaaaaa aacacccgct atgcgggtgc
    45481 gcgtcgaagg ttataccaaa aaaactccaa acgcgataca ataaaggtgt tcaagccaat
    45541 tgtaaagcga aaggagaaaa atatggcaca aaaggttctt ttgtcctgtt cttgtttcaa
    45601 ttgactatat ttctatactt tttcttcctt tctctgtgaa aatcgcaagt ttttcccacc
    45661 ttttctcaat actgaaacaa catctttcca gaacttcaat aatttctttt tgtgttaaat
    45721 cattatttaa ctaaatcttt ggaggcaata cgttttcaaa gcgacttttt taccataggt
    45781 tttgagcctt caacacagat tactaagcca aaaatgctct aaatttttga aattctaaca
    45841 aattccactc aactttatca tcatctttga ctttcacaat atcttcctca aaataacatg
    45901 gaataaagat agttttcttt tgaaacggtt gtaggaggaa aatctctcct acactattat
    45961 tgattctgag aggaaattgc aaaatatcat ctaccaaatt ttcaattatt aagatttttt
    46021 gcagtttatc tgaattagaa tcgcaaggct tctcaataat ataattgtcg aattttttca
    46081 ttctaacttc ctctccacta tttcctcctt tatcaagaaa aagttacctc aatattaata
    46141 aagtacaatt ctaaattaac tccaattcta aattaactcc aattcttgat tcatgataat
    46201 atttcagtaa ctcatcgtgc aattaaaaac aaggattttt atttcagatg atccatcgtg
    46261 aacacttcat cagccatctc ccaaattgcc ggattatgtg ttgcgataat gattagccta
    46321 ttatcatctc gaagagatag caaaatctcc ataatcaact gagaggttgc tgggtctatt
    46381 gaagctgttg gctcatctgc cagaataaag ggtggattct ttaagataat ttttgccaag
    46441 gcaactcgtt gcgattctcc gcccgataac tcaaagatgc gcttatctag gtcaagataa
    46501 gccaggccga cctgttctaa agcctgcttc tgcctcaacc gctgttccga ccgactcaac
    46561 ttttgaccaa tgagacctag cttaaggttt tcttcaatac tttggttttc aattaagcca
    46621 aagttctgga agaggtagcc caattcgtga cggaaaaaat cactcgattt ataattggcc
    46681 aagtctttac ctcggtaaaa aatcgtccca tcataaggtt ctaatttccc aatcatgttc
    46741 atcaaggttg tttttccgct accacttgaa ccaattaagg catagacttt tccagcctca
    46801 aatgtcattg aaagattcga aaataactct cgttctccaa aagatttact cacctgttta
    46861 agttcaatca tattaacctc ccttcaaaac aagcatggac atcttgtttt ctttctgcat
    46921 ttggacatgt aactgtaaaa gagatagacc agtaaagagt aacaagacta agaaagcaac
    46981 tcctacctct acctgaagaa atacactcgc aacaaatccc agtaaaaaca cacccagttg
    47041 agcaaagaga taagtgcgat ggatttctaa gaacctgaga cctgcaatgc gtttgataaa
    47101 aatggcacgt ctaaattctt caaagtagag cctattcata gtgttaaaca acaagattga
    47161 agttgcaatc ccaagcacag ctcctgctag cattacccaa cgttccctct ggatattatc
    47221 caataatgtg atgtagttgt ggtaacctgt ttgcatttct gagacccaat tttcaatgcc
    47281 ttgtctctgg ataagctcct gggcatcaga caattgatta aagagaacgt agttctgtac
    47341 tgcgtctatc caaaacaaaa tggactgtgg accagttgat tggggtgtta taacaatgat
    47401 gattggatct ttcaaaaact gctggtaaga aataggggtc gtattataca caaaacgatc
    47461 ctgacctgat tctaaataac ctaccgtagc agtcatttgc tgtcgttcat cttgactaga
    47521 catgcgactg gttaagtcgt cttcaaaaac agatttataa tgttcttcct ctgaacggag
    47581 gtgttcaggc agcaataaga caaactcccc tacatcaagg tgattcaact tttgactcat
    47641 tttagttgaa ctgtaggact tactttttca gagggctcca aattttattt tcatttactt
    47701 tttttagccc ataaaatatt taccatactt ataaaaagta gtaatactcc aactaaactg
    47761 acttgtaatg aattatatgt attttctgaa gtgttaggta aataattttg agagtaccct
    47821 tctcgggcaa tagtaataat attgtgtgtg tttatttgtt taattccaat agtagtttga
    47881 attctctctt ccaactcaat taaattaaca ttatctatga aactatccca actttgttca
    47941 tttttatatt ctaattgtaa catattcaaa tcatatctca atacgtttgg tatatttaac
    48001 acttttatac cattttttac tggtaatgtt tcttccagaa ttttttttac aaacatataa
    48061 ttatagggct gatcttttaa tttgtattta tcaataatca ttgcaatcgc accagaaact
    48121 ttaggagcag caaatgagtt tccataaaga tacgtatatc ctcctagtgt tgccgaaaaa
    48181 atccaatctt ctttatattt ttctgcatta ataaagttat taagtcctaa ttctgaaaga
    48241 gataatgttg agcctgcagg cgcatatatt gcatcagaat cccctttatt gctaaaatct
    48301 gataaattac ctaagcgatc tatgcctccg accgaaattg tagatgagaa ataacttgga
    48361 acatcaacta ctaatcctgg ttttcttact tttttgcgtg aactaataag tttcaataaa
    48421 tctgactgat ttgatacatt tagggagtca ttccctaatg cagctactat aattactcct
    48481 ttttgattcg cgtaatcaat agccttttta tacttcaaaa atgtttcaaa atcatttgtt
    48541 ccgtcctcat attctccatc aatcattaaa tattgtccag tactaagatt gataatatca
    48601 ttgccatcat ctacagcatc aaaaattgct tttacaatcc aatctggact agctgacgac
    48661 ttaccaaata ttctatagac gttaatatta acgtgaggat ttactccatt aatattgtca
    48721 tcccctacaa tttgagctac aacagccgta ccatgaccta atctatcttg aatatctgat
    48781 attattcctt cctctccctc ctcttttcct aaatatcctt tattggggac atagtttttt
    48841 gagtgatttg aaagattttt cagtaatcca gtattttgta aatcgactcc tgaatctaaa
    48901 atagcaattg aaactttaga attactttta gaatataatt tataactttc gcctccacca
    48961 gtaactttat ctatatccca ttcatatttt cttacaatcg acgattctaa gttatctttg
    49021 tcatgctcta tatttcctcc tctattatct agatgagaaa cattttcctt aattttatta
    49081 tctaaattat cttcgcctag taaattatta ctttcagagt taacaatatc gttatttatt
    49141 tgtggttcaa gttccaattt acaacagttt ttttctaaat gtttatctgt tgttacaaat
    49201 aattctgtct tatcgatttg aatacgaaat ttattttggt ctacttcttc ttgattttta
    49261 ttagaatagt caacctgttt ttcatcagat tcagaaaaac tttcgctagt ctgagtgtga
    49321 gttatattgc catcaagatt tttaggtatt tcttgagcag taacagaatt attgaacaaa
    49381 caaaagaata atatcgctaa agtccaatat ttttttttca taatcttcat aaatttaccc
    49441 ttttaatcaa aaactaatta tttgaataca attgcttata aatgcctcct agttcaagaa
    49501 gttctctatg ggtcccaatc tctacaattt caccattatg cattactaag atcttatctg
    49561 cagacttgat agtatctagt cgatgggcaa ttaaaatctt agtggtaccg atttcactta
    49621 agtaagaagt tgtaatttgt tgagataaat tatctaaact acttgttgct tcgtccaaaa
    49681 caactaccga aggtttcgac aaaagacaac gagctaaaag tatcatttgc tgctgccctc
    49741 cagaaaagtt attaccttct tcagcgatga ttgtctctaa tccaagaggt aaagatttaa
    49801 ctacattgct aagaccaatt gaatttagat ttgtcattaa ttcttcatct gaaacaaagt
    49861 gtccaaatgt caaattctct cttaaggtac cataacttag ggttgaattt tgtaacactg
    49921 ctccaaaaat ctttctatac aatgtttgac caatttcttc tttttctact ccatcaactt
    49981 ctattgaacc aatattaggt tctaataaac ctaataaaat tttagctaat gtactcttac
    50041 ctgaacctga ttctccaaca atagcaacag tttctccttt tttaatagta acatttattc
    50101 ctttcaaaac ataatcatca aataatccat atttatacca aatatctttt aattctattt
    50161 ttttatcaaa ttttatatct tgactgactc tttctggaat taattcggat ttagtattaa
    50221 acacatcctc tattcttaaa aaatatccct ttaataacat taattgtata tagttatcac
    50281 ttaaagaaat tataggagaa ataaagtatg gtgagactgt acttattgct acaatttgtc
    50341 ctaacgtcaa ttgttcgaat gtttttatat ttacacctac aataagggtc aaaacaggga
    50401 gaataatttg taaaacattc gttatactac taacaattga taaatgtata tcaagttttc
    50461 gacctatgat gagctgtttt gtattaaaat tatcccattt gttaatccaa aattcctctt
    50521 ctccagttag cttaatatca ctatttttag aaattacttc ggaagtaata ttttgaacat
    50581 taaccttttc ttttatattt ttatcaatta aattttttga gatttttatg attggataca
    50641 ttacaataga tagagctaga cttagtgata ttaaaaagat taccatgtac ttagaaaagc
    50701 taaataaaac cacagcataa accacaatca ttaacaaatc aagtatagct gctataaaat
    50761 tatttgatag tatttctctt atataaacaa tagagttagc tctaaaaagt atatccccta
    50821 aagtacgttt ttcataaaaa ctgtaaggta aatatatcaa atgtttcata aaatcatagc
    50881 taatctcttt atccattata tattttaagg atgcaacata tatctgtctt aataaataca
    50941 ttagtgagaa tgaaacagta aatataaata atatagtaaa taacattcta gacgaatacg
    51001 aatcgtcctt gaaattcgtg tcaattatgt atctattagc tataggtact aatgattgta
    51061 ttacatacaa taatgctgtt acaaaaaata aaatccctag cttattccta tacttaaaaa
    51121 aatactttaa aacaggcgac ttcttattat ctttacgaga cataaagcta tctaactttt
    51181 taaatgttaa aatgatattt gaataatgcc tttcaaattc acttattgac attctctgcc
    51241 tgccttttgc aggatctaaa atataaaact tgtttttact aattttttca agaataataa
    51301 aatgagaact ctcccagaag caaatgagtg ggaaactgag cggttttaaa tctcttaccg
    51361 aagctttaaa agcatgagaa cttatattaa aatctcctaa aactttactt atagatagga
    51421 gagaatatcc agatctggat gatgggtaga tttcacgtag ttcatttaaa gaaatctcat
    51481 atttaaaata gttcaatatc atggcacagc atgctaatcc acattctgaa ttttccattt
    51541 gatatatcat tggaattttc acaatacttc ctccttgttc aaaataaagt cgataatttc
    51601 ttcttgtcta aaatctacta atttctttag atacttaatc ttttctaaat atttattatt
    51661 tttcgagtaa atatagtttg acaaggtaaa aacaacttct tctaaagata agttctgtgg
    51721 aataatccaa tcagaagatt tatgtagtaa tttatagtac tcatggataa tattttcctg
    51781 aatatttgaa tcaaaaactt catgattagc aattgtaaat gctgaccctt cacctttcac
    51841 ttcaaagtaa aggtttatca aacatttttc aaattttgaa aaaaaagatt tttctttaga
    51901 ggttaatttt gcaaaatcgt cgattgttat ataaggatta ttatagagac tgttcgcaaa
    51961 gaatctctga tatgtttttg aatcttttga atacaaaact atctctctaa tagcattgcc
    52021 atctgttcca tcaattggta aacataccgt aactagaaaa agaattatat tcaaaataaa
    52081 aaattctgat gcgtacggca caaatcccaa aagtgctaat attgcgacaa ttaggttagc
    52141 tccacctccc ccaaagaagt agaacaccaa attcctatca ctattttttt cattagtaat
    52201 gtttctatta ctcatttgac aataaccgaa tgctaataac actggaaatt tgaaatatat
    52261 tttttttctg aaatagaaga aaaagggagt agcaagcatc tctagtttat aagataaaca
    52321 tcttcccact aaaaaatgac ctagttcatg taatgtaatt gatattaacg aaattaaaat
    52381 caatcgaaaa taatagatta atgaatcatt tggaaaaatt atcaataata ggaacaataa
    52441 cggaatcaaa cataaatata tgacagagtt atttaatatt ttcaacataa taccattcct
    52501 ctaaactatt agcttcaaaa aggcgttttt tctcccaata catcttctca aaatgttcgg
    52561 aatcataatt ttctaaaatt aattttaatg tctggtaagc tctttcttga taatccgttg
    52621 ttttgtactt aattttccct tcaagtacat cttcaatttt ataagttgcc tccatcaact
    52681 gagcctctgc aatatctttg agtgaattgg taattgaaac ttggtgtaat atctgtcctc
    52741 ccatatatga aaatatatct ctaagatatt ctgacacatt atcagagccg ttactctcag
    52801 caacatctaa tgttacaaca aactttccag ctaatcgaaa aagatggctc caccccccaa
    52861 tcctttcaat aaagtttttt gtgtccacag atacgttttg taaatataca ggagaagaga
    52921 taattataat atcagactct aataactctt tttttataac acctccatca tcagcattac
    52981 tttgcctatc aattcctttc ttaaacaact cttctgaatc agaattagat atttctagct
    53041 ctgaattgaa aggtgtcctg aaagatatat caacattatt tctactagaa atgatacttg
    53101 aaagtctctt agtatactct aaagtcttag agttatgatt tcgcactcct gcatatataa
    53161 atattttatt cattttaatt catcctctca atttgaattt agtagatttt tcaagatagt
    53221 atggtacaaa aacagacttt tgttgactca cattattaca tatgttttgt attaaaccaa
    53281 aatcaatact atttttggag taattttgat tttagtttaa aatcatttct ataacagtag
    53341 catatacctc aagccgttta gcaattagaa tagaaccttt ctttattata ttattatctc
    53401 aacgaaaagc tacactatta aaaatatttt atagaattac atattaaact agtcaatctt
    53461 ggtattttta tattgcttaa tgagtggaca cctctatttt agaaacaaaa ctataaatta
    53521 agctagattt caagtaatga ggggataact atctttttgt cattctgatt cagtgcgata
    53581 taccttaaaa aagtataagc aataccagtc acacctgtat acaaagaaaa atctgggaaa
    53641 ttgcttgttt ggacgatacg atactctcct tcttttgatt tattcattac aacactacac
    53701 aataaagact ccaattccat actagtatcc atttctttca tgtagtcgat gtaaaaattt
    53761 attatggcca tacttccatg gcaaaatgta tcattatcta aactagctac aattccctct
    53821 ggaacacttt ggggatgatt aactaatgtc ccaaattctc cactacacca cttcaaagaa
    53881 tgaattttga ttttctccct aggaactagt tgtaaaatta attctttata ttttttaagt
    53941 cttgtcactt tataaatatt ttttaatgta aaaattacac ctgatagtcc atggccaaaa
    54001 ctatatccaa aattactatt atctctctcg cttacatcat tatatagcgt atcacctaaa
    54061 cttaatacta gccttagaac acgttccttc tctattcctc tcctataata tcttaccagt
    54121 gtattaatta aaggtagaag accattaata tagtcagact tgtttgaaac acttgcaaaa
    54181 tcagtctttt caagctcagt taaaacactc tttatataat ttaagcatgc gagagtattt
    54241 gtatcgtaat cctctataat ggatagaaca atgaaatatc ctatatcccc agttaaacca
    54301 aatgtggtct tagataaaga aacagatggc ggaattgcag ataacatttt attgtacagt
    54361 tgagtatatg atgatttatc tttcaataat tttacatagt acataaacag taatattcca
    54421 gctctacccc tatacatatc attacccgtt tgttcaagac accatttaga acctttaaaa
    54481 ttaacaggta tactccaaat tggatattcg tcataaatat tattaataac caaagagtct
    54541 gcaatatttt ctacttcatt atgcagaata gtaactaaac tttcatttgg gagttttttt
    54601 tctattagat aagtttaatt tatatccttt ttttcgctga tcaaagcttg gaaaataaat
    54661 ttcaatgata tcaagttgct tttctaaatt ttccaaatta ttattaggta aatatttcat
    54721 aaaatagtca tatccagaaa attgatgtag ggaaataaaa tgatttccaa aatcatcgta
    54781 gatttcattg atatttgtat ctgtataaaa aatcggaata tctaataacc tcatttgttc
    54841 acattcgctt gctacaatac cttgattaga aaacttattg ctccagagat tttccaatgc
    54901 tttttctcta tctaacattt cttcataaaa atcaggatga tataaaaaag atagtactga
    54961 agcatagcta tttgtgtctc taaaaagtac ccttgtcttt aaaccataca agtttgcttt
    55021 taatagcatt ttaaattctt ctgttttatt taactcttca aatatcagat aaaaatccct
    55081 aaaacctttt ttgaaatctt ttatatactt atcaaattct atatcaccat cccgaacagg
    55141 caggtttttc ccaccttcaa aatcaatttt cccaatatca aactttacct tatcagtatt
    55201 taaattaatt aaaacttgac cattaaaagc atccttctta aaatttcctg taagagcact
    55261 catctcaaca tcaagaccat cctccctttt tactccctct gttggaagaa gcaaagttcg
    55321 agtcaccctg tgaaacaggg tatccagcaa actctgacca gttttatcat caaatagagg
    55381 ttgctgtaac attgtttcaa agtctattat tacaggcata tctccatagg aaattaaatt
    55441 ttcaaaatgt atatcactcc cattaaataa atatataaat gctaaaagca caccaaaatt
    55501 ataataatat tcaggtaatt ttttcttttg ctctagaggt agataatcaa taaattcttc
    55561 atagctgtga tccgaataag caatagttcg aggtattctt atcctaattt tttcatcaac
    55621 ctttcggata tactcagcaa tagtatttag tgagttattg attattaaat ttttaggctt
    55681 gtaaacaatt ttcgcgtcat caaagaagag tatcaaaaca gtttttcctt ggctgtgtgt
    55741 atcacccttt ccaaatttta tatcatttaa tttccccctt agccctaatt cttcttctag
    55801 cacactaaaa tcattttcta aatttgataa aatttctata aaataagtat agaaccgaag
    55861 acaagactcc gatatttgcc tcagtaaaac gggatatttg ttataaaaat ttaatatatt
    55921 ctcttccttg tcaaacaatg ttataaaata tttgtaccgt tgctgctccg attccccttc
    55981 aagtaaacct ttttcacgca atgaatttat ctctaaaacc aaaggtttaa caatccaagg
    56041 atagattaaa tcttctaaat aaaatactag agaggaaaaa atatttccat tcttgtcaat
    56101 ccactcgtat tctttattct ctagataatt attgattaat ttaagcaaat taataataaa
    56161 gggttccaaa attctagaaa aatctttctt aaaattaatt ctttggggat gttttctaaa
    56221 tatttcaaat gaaccctccg caaatttata ccattccgaa tttttggcat attctgaata
    56281 tagtcttatc tgttcctcgg tataatcata gttcacagtg ttagaaaata tttttttatt
    56341 gtaattattt aatttcaaca tttcttcaaa agttttttca gaaataattg acattgtaga
    56401 acgccaatta tttaatgaaa agttatttaa ggaattatca tttcgttcag ataatatttg
    56461 atatctttcc ttaataaaca atgttttatg cacattttta attttatatt tcatttactt
    56521 acctcaaacc tatacgactt actattgttt taaaaaaaaa acctctctca aaattattag
    56581 ttacgagaga ggaaaataaa gagcaacttt tatttttaaa attgaatatt ttattttttc
    56641 ttgttgtctt tgccacaacg agtctgagct gaccccaaat atgatgcaat aaaacttaat
    56701 gtagcagaac atataattgt tgatctaggg tcagcaccat ctgtgccacc ataaaccaat
    56761 tgcatttctt caagagataa ttctttcaaa ctctttccga ttacaaaatc atttttcatt
    56821 ttaatttacc ccaaacaatc attattgtag ctaactagcc ccccaatacc agaaacagct
    56881 gcactaatgc atactttcga gctagctgca gctgatttca aaataatagg agtaggcgtc
    56941 caacgtggct ctgcaccatc agatgcacca taaattaaac tcatttcatc agcagacaat
    57001 gattttaaac tttttccaca aaatttttcg tttgaattaa agttggtcat aagtaataac
    57061 tcctccttat aaaaagttaa ataagttgct ctatattttt cgttttttca gataaattat
    57121 aattctctca ttctcgtgca aaatatcggg ttttaatccg ccttcccaac atcaagacca
    57181 ttggacttct tttgcgattg cactcgaatc tccaattgtt tattcttctg aataaactgt
    57241 tggtaaggaa ttgtattact aaacataaaa acacctagca atgcaaatgt aactaatttt
    57301 ttaattccac gtttttttct catgcaatga aacccctttc tttttccttg tttttattct
    57361 aacagatata ttttatttag tcaagtattt ttatgtaatt tttaactttt ttttttgttt
    57421 attaccataa agaattattt attcctttaa cttgctattt tgaactaaaa actttataat
    57481 gaaattaagc aaataggaag gtttattatc tttaatgaat acactcgcag agaaattcag
    57541 attgaaaaga aaagagttga gactctccca acaaactctt gcagaaggaa tttgtgaaca
    57601 aagtcagatt agtaaaattg agagagggca tttcattccc tccgcagacc ttttgttcaa
    57661 actctctcaa cgacttgaag taccattaga ttattttttt aatgaacaaa ttgaaattaa
    57721 atctaacctc tctaatttca agcaattgtc tgctcgacta ttagatgaca gaaattatga
    57781 cgatttagaa tatctttata aaatagaggt agaacgaagc acttttctaa cattagaaga
    57841 ccgaacttac cttgaatgga ttaaatctat aattgacttc tatcaatatg acagtaagtg
    57901 tgaggcaatt tcgtcattgg aaaatatatt attaaaagtc tcttcaaata ctctgattta
    57961 tttaaaggcg ttgaatactc tatctaattt ctattcctta gtgggtcgtg aacaagaata
    58021 tgaggcaaac tactctcatt taatagagtt gtatcagaca aaaaatttgg accatcaaga
    58081 gtttttattt ggctacatca gagttcgtta caactacgct cactacctag tatcaaagga
    58141 aaaatataat gaagccatcc aagaagctct tgaaacgatt gaactctgta aacaaagaca
    58201 gacaagctac caattggctc ctctacttat tcttgtagga aatgctggag ccaaatttct
    58261 agacagagaa caagtcaaaa attattatat agaagcaaga gagttatgta agatttataa
    58321 caatccttta atgttgatga agatagaaaa ttatttgaag gaactagata ctgtttagtt
    58381 aatcttgaca ttatagtttt tctgaaacgt taaataacct gtaaggctga tagttagatt
    58441 aatactatca gccttataat tatgtgactc tgagtccgtc tcgctccgtg aggtgcgaga
    58501 caaaacaacc acccgtttga cggattttca tgatttcaaa tttatattct tcgaaaatct
    58561 cttcaaatca cgtcagctct atctgcaatc tcaaaacact gttttgagca acctgtggct
    58621 agctacctag tttgatcttt gattttcatt gagtattagt ctctagtgtt tgtttagcgt
    58681 cttaaagaga catttgagaa gattagaaac agttctctct tttctcctag tataatgatt
    58741 ggtaggaagg gagagagaag atgaaatcaa tgagaatctt atttttgtta gctttaattc
    58801 aaatcagttt gagtagctgt ttcctatgga aggaatgcat cttgtccttt aaacaaagta
    58861 cagctttttt catcggaagc atggttttcg tttcaggaat ctgtgctgga gtaaattatc
    58921 tttatacccg taagcaagaa gtccatagtg tcctagccag taagaagtcg gtgaagcttt
    58981 tttacagtat gttactctta attaatttgt taggagctgt tcttgttttg tcagataact
    59041 tgttcatcaa aaatacgctg cagcaagaat tagttgactt tttattgcca tccttctttt
    59101 tcctatttgg gctagatttg ctgatttttt tacccttgaa aaaatacgtg cgcgattttc
    59161 ttgctatgct ggacagaaaa aagacagtgt tggtgactat tttagcaaca cttcttttct
    59221 taagaaatcc aatgaccatt gtctcacttc tgatttatat tggactgggc ttgttttttg
    59281 cagcctatct tgtcccaaat tcggttaaga aggaagtttc cttttatggt catattttcc
    59341 gagatcttgt attggtcatt gttacgctca ttttctttta gagaaagcct gttttttatg
    59401 gatgttatga tgataagttt agtaggaaaa tagacacaaa agaaaagttt ttggtataat
    59461 aagagtatgt acacaaaaaa tgaagaagag ttgcaagcct taggggagcg tttgggccat
    59521 ctattagcaa agaatgatgt tttaatctta actggagaac tgggtgcagg taaaacgacc
    59581 tttactaaag gacttgcaaa aggattacag atttctcaaa tgattaaaag tcccacctat
    59641 actatcgtga gagagtatga aggtcgactt ccactttatc acctagatgt ttatcgtatt
    59701 gaaggagatg ctgattctat cgacttggat gagtttatct ttggtggcgg cgtgactgtt
    59761 attgagtggg gaaatctctt aggagatgcc ttgccagatg cttatttaga attggaaatt
    59821 ctaaaagaag cagatggacg ccgtttaaat tttcaggcaa agggtttgcg tgctgagaaa
    59881 ttgttagagg agcttcaata tggagtatga attgctcatt agggaagcag agcccaaaga
    59941 tgcagctgaa ttagtggcct ttttaaatcg tgtgagtttg gagacagact ttaccagcct
    60001 agacggagat ggtattctct tgactagtga ggagatggaa atattcctca acaagcaagc
    60061 tagttcggac aatcagataa ccttacttgc attcttaaat ggcaaaattg ctggtattgt
    60121 aaatattaca gctgatcagc gtaagagagt ccgtcatatt ggagatctct tcattgtgat
    60181 tggaaaaaga tattggaata atggcttggg aagtttgttg ctagaagaag cgatagagtg
    60241 ggcacaagca agtggcattc tgcgtcgtct ccaactgact gtccaaactc gtaatcaagc
    60301 agcagtccat ctttatcaaa agcatggctt tgtcattgaa ggtagccaag agcgtggggc
    60361 atatatagaa gaagggaaat ttatcgatgt ttacttgatg ggtaaactga taggttagta
    60421 gaaatatggt taaaaaaatt attggaatgg tgctagcttt actttctgta actgtagtag
    60481 gagtaggtgt ttttgcttat actatttatc aacaagggac agaaacctta gctaaaacct
    60541 ataaaaaaat cggtgaagaa accaaggtta ttgaagcgac tgaacctcta accattctgt
    60601 taatgggagt ggacaccgga aatgttgaac gaactgaaac ttgggtcggt agaagtgata
    60661 gcatgatctt gatgacagtg aatcctaaaa cgaaaaaaac aacaatgatg agtttagagc
    60721 gggatattct gacgcgcatt gaatcaggga atggtcaggc tcatgaagcg aaactgaact
    60781 cagcatatgc agatggtgga gcagagcttg ctatagaaac cattcaaaaa atgatgaata
    60841 tccatattga tcgctatgtg atggtcaata tgagagggtt gcaaaaatta gtggatgcag
    60901 taggaggtat tacagtcaat aatatcctag gtttcccaat ttctatcagt gaccaagaag
    60961 aatttaatac catttctatc ggtgttgggg agcaacatat tgggggagaa gaagccctag
    61021 tctatgcacg aatgcgttac caagatcctg agggggatta tggtcgtcaa aaacgtcaac
    61081 gtgaagttat tcaaaaagtc atggaaaaag ctctcagttt aaatagcatt ggtcattatc
    61141 aagagattct aaaagctttg agtgacaata tgcagaccaa tattgatttg tctgcaaaaa
    61201 gtatccctaa cttgctaggc tataaagatt catttaaaac cattgaaact cagcagttgc
    61261 agggtgaagg agagatactt caaggtgttt cttaccagat tgtttcgaga gcacatatgt
    61321 tggaaatgca aaatatactc cgacgttctt tgggacaaga agaagttact cagcttgaaa
    61381 ccaatgcggt tttatttgaa gatttatttg gcagagcacc tgttggtgat gaagataatt
    61441 aacttttgat acaaataaaa aaatcaatcg taggaaaatt cctgcgattt tttcaaaaaa
    61501 atacgaatag ataggtagga ggaaacatga aagcagaaat cattgctgtt ggaacagaga
    61561 ttttgacagg acagattgtc aacaccaatg cccagttttt gtcggaaaaa ctagctgaga
    61621 ttggggtaga tgtatatttt cagacggctg taggagacaa tgaagttcgt ctcttgtctt
    61681 tgcttgagat tgccagtcaa cgtagcagtc tggtgatttt gacaggcggt ttggggccaa
    61741 ctgaggacga cctaaccaaa caaaccctag ctaaattttt agggaaagca ttagtctttg
    61801 atcctcaggc tcaggagaag ttggatatct tttttgccct gcgaccagac tatgcccgaa
    61861 caccgaataa cgaaagacaa gctcaaattg tagaaggagc gattccactg ccaaacgaaa
    61921 caggactggc tgttggagga aaattagaag tagacggagt gacctatgtc gtccttccag
    61981 gtccgccaag tgaattgaaa cccatggtct taaaccaact tctacccaag ttgatgacag
    62041 ggagcaagct gtattcccga gttcttcgtt tctttgggat tggcgagagc cagctggtta
    62101 cgattttggc tgatttaatt gataatcaga tcgatcctac cttggcccct tatgccaaga
    62161 caggagaagt cactctacgt ctgtcaacaa aggctagcag tcaagaagag gcgaatcaag
    62221 cgctggatat cttggaaaat caaatcttgg actgccagac tttcgaagga atttctttac
    62281 gagacttttg ctatggttat ggggaagaaa ctagtttagc cagcattgtg gtagaagaac
    62341 tgaaaaggca agggaaaacc atcgcggctg cagagagttt gacggcaggt cttttccaag
    62401 ctaccgtggc gaatttttct ggagtttcaa gtatatttaa gggtggtttt gtgacctata
    62461 gcttggagga aaaatcaagg atgttggata ttcctgccaa gaatttggaa gaacatggtg
    62521 tggtgtctga atttacagct cagaagatgg ctgagcaggc acgaagcaag acccagtctg
    62581 attttggaat tagtttgacc ggagtggcag gaccagatag cctagaagga cacccagttg
    62641 ggacagtctt cataggcttg gcgcaagagc aaggaactga ggttatcaag gttaatattg
    62701 gaggcagaag ccgagcagat gtacgtcata ttgcggttat gcatgccttt aacctagttc
    62761 gcaaggcttt attaagtgac taacttttga tataatagta gataggtctg aggatcatta
    62821 gaatgtagga gaatagaatg gcgaaaaaac caaaaaaatt agaagaaatt tcaaaaaaat
    62881 ttggggcaga acgtgaaaag gccttgaatg acgctcttaa attgattgag aaagactttg
    62941 gtaaaggatc aatcatgcgt ttgggtgaac gtgcggagca aaaggtgcaa gtgatgagct
    63001 caggttcttt agctcttgac attgcccttg gctcaggtgg ttatcctaag ggacgtatca
    63061 tcgaaatcta tggcccagag tcatctggta agacaacggt tgcccttcat gcagttgcac
    63121 aagcgcaaaa agaaggtggg attgctgcct ttatcgatgc ggaacatgcc cttgatccag
    63181 cttatgctgc ggcccttggt gtcaatattg acgaattgct cttgtctcaa ccagactcag
    63241 gagagcaagg tcttgaaatt gcgggaaaat tgattgactc aggtgcagtt gatcttgtcg
    63301 tagtcgactc agttgctgcc cttgttcctc gtgcggaaat tgatggagat atcggagata
    63361 gccatgttgg tttgcaggct cgtatgatga gccaggccat gcgtaaactt ggcgcctcta
    63421 tcaataaaac caaaacaatt gccattttta tcaaccaatt gcgtgaaaaa gttggagtga
    63481 tgtttggaaa tccagaaaca acaccgggcg gacgtgcttt gaaattctat gcttcagtcc
    63541 gcttggatgt tcgtggtaat acacaaatta agggaactgg tgaccaaaaa gaaaccaatg
    63601 tcggtaaaga aactaagatt aaggttgtaa aaaataaggt agctccaccg tttaaggaag
    63661 ccgtagttga aattatgtac ggagaaggaa tttctaagac tggtgagctt ttgaagattg
    63721 caagcgattt ggatattatc aaaaaagcag gggcttggta ttcttacaaa gatgaaaaaa
    63781 ttgggcaagg ttctgagaat gctaagaaat acttggcaga gcacccagaa atctttgatg
    63841 aaattgataa gcaagtccgt tctaaatttg gcttgattga tggagaagaa gcttcagaac
    63901 aagatactga aaacaaaaaa gatgagccaa agaaagaaga agcagtgaat gaagaagtta
    63961 cgcttgactt aggcgatgaa cttgaaatcg aaattgaaga ataagctgtt aaagcagtgg
    64021 agaaatccgc tactttttcg atttttgatt caagttttta gattatatat agtagcttga
    64081 aataagatat gaacaacttt attaggaaag tcaaattaat ttctagaaat gttttagcag
    64141 ctacagcgta ctattccaaa ctcaaccaat tataatagat cgaaactaga atagtacaca
    64201 tctacttcta aaacattgtt aaaaatcgat ttgactgtcc ttatttcatt ccgctatata
    64261 tagtttgctg tttcttgtcg ctcctctgga aagctgatat aatagcttta tgaataaaaa
    64321 acgaacagtg gacctgatac atggtccgat tcttccctcg ctcttaagct tcgccttccc
    64381 aatcttgcta tcaaatattt ttcaacagct ctataacact gctgatgtct tgattgttgg
    64441 acgatttctt ggtcaagaat ccttggctgc agtaggagcg acgacagcga tttttgacct
    64501 gattgtaggt tttacacttg gtgttggcaa tggcatgggg attgtcattg ctcgttatta
    64561 tggggctcgg aatttcacta aaatcaagga agcagtagca gctacttgga ttttaggtgc
    64621 tcttttgagc attctagtta tgttgttggg ctttcttggc ttgtatcctc tcttgcaata
    64681 cttagatact cctgcagaaa ttcttcctca atcttatcaa tatatttcta tgattgtgac
    64741 ctgtgtaggt gtcagctttg cttataatct ttttgcaggc ttgttgcggt ctattggtga
    64801 cagtctagca gccctgggat ttctgatttt ctctgccttg gttaatgtgg ttctggatct
    64861 ctattttatt acgcaattgc atctgggagt tcaatccgca ggacttgcta ccattatttc
    64921 gcaaggttta tcagcggttc tctgctttta ttatattcgt aaaagtgtgc cagaactctt
    64981 gccacagttt aaacatttca aatgggacaa aagcttgtac gcggatctct tggagcaagg
    65041 tttggctatg ggcttgatga gttcaattgt atctatcggc agtgtgattt tacagtcttc
    65101 tgttaataca tttggtgcag tgattattag tgcccagacg gcagctcgac gcattatgac
    65161 ctttgccctt cttcctatga ccgctatttc tgcatcaatg acgacctttg cttctcagaa
    65221 tctaggagct aagcaacctg accgcattgt tcaaggtctt cgaatcggca gtcgtttaag
    65281 tatatcctgg gcagtttttg tttgtatttt cctctttttt gccagtccag ccttggtttc
    65341 cttcttggct agttcgacag atggttactt gatagaaaat ggaagtctct atctgcaaat
    65401 cagttcagcc ttttatccca ttttgagcct cttgttgatt tatcgcaatt gcttgcaggg
    65461 cttggggcag aagatccttc ctctagtttc tagctttatt gaactaatcg gaaaaatcgt
    65521 ttttgtggtt ttgattattc cttgggcagg atataagggt gttatccttt gtgaacctct
    65581 tatctgggtt gccatgacag ttcaactgta cttctcacta ttccgtcatc ccttgataaa
    65641 agaaggaaag gcaattttgg caaccaaagt gcaatcctag ttggatttac tgaataaaat
    65701 ccatttcctc tagtgaaaat cgaaaaaact tgtgttctct tctttagttt ggtgttgaaa
    65761 atagtttaac agacttttga cttcttttat atgatataat aaagtatagt atttatgaaa
    65821 aggacatata gagactgtaa aaatatactt ttgaaaagct ttttagtttg gggtgttatt
    65881 gtagatagaa tgcagacctt gtcagtccta tttacagtgt caaaatagtg cgttttgaag
    65941 ttctatctac aagcctaatc gtgactaaaa ttgtcttctt tgtaaggtag aaataaagga
    66001 gtttctgttt ctggattgta aaaaatgagt tgttttaatt gataaggagt agaatatgga
    66061 aattaatgtg agtaaattaa gaacagattt gcctcaagtc ggcgtgcaac catataggca
    66121 agtacacgca cactcaactg ggaatccgca ttcaaccgta cagaatgaag cggattatca
    66181 ctggcggaaa gacccagaat taggtttttt ctcgcacatt gttgggaacg gttgcatcat
    66241 gcaggtagga cctgttgata atggtgcctg ggacgttggg ggcggttgga atgctgagac
    66301 ctatgcagcg gttgaactga ttgaaagcca ttcaactaaa gaagagttca tgacggacta
    66361 ccgcctttat atcgaactct tacgcaatct agcagatgaa gcaggtttgc cgaaaacgct
    66421 tgatacaggg agtttagctg gaattaaaac gcacgagtat tgcacgaata accaaccaaa
    66481 caaccactca gaccatgtgg atccataccc ttacttggca aaatggggca ttagccgtga
    66541 gcagtttaag catgatattg agaacggctt gacgattgaa acaggctggc agaagaatga
    66601 cactggctac tggtacgtac actcagacgg ctcttatcca aaagacaagt ttgagaaaat
    66661 caatggcact tggtactact ttgacagttc aggctatatg cttgcagacc gctggaggaa
    66721 gcacacagac ggcaactggt actggttcga caactcaggc gaaatggcta caggctggaa
    66781 gaaaatcgct gataagtggt actatttcaa cgaagaaggt gccatgaaga caggctgggt
    66841 caagtacaag gacacttggt actacttaga cgctaaagaa ggcgccatgg tatcaaatgc
    66901 ctttatccag tcagcggacg gaacaggctg gtactacctc aaaccagacg gaacactggc
    66961 agacaagcca gaattcacag tagagccaga tggcttgatt acagtaaaat aataatggaa
    67021 tgtctttcaa atcagaacag cgcatattat taggtcttga aaaagcttaa tagtatgcgt
    67081 tttcttgtgg agatatttcc ttcaatgttt gctactatat taaacaaaaa tcagaaagca
    67141 aactagaaag ttatgctcaa ataaaatcta aatttgacaa tgtaaaccga gtcggatagc
    67201 tttaagtact gttttgaggt tgaagatacg atttttgata ggaactcatc aaataggttt
    67261 aatatcatca atcttagatt tttaagcagc atcaataaat tgcttccttg ttttgtcata
    67321 atttttttat ttaaaaaatt atgacaagag tgtgctattc tttttatgag aggtgtatga
    67381 atatgataaa tgtatgtgat aaatgtatgt gatgttggaa aaagaataaa agaacttaga
    67441 atatcttcaa atcttactca agataagatt gctgagtatt tgtctttgaa tcaaagcatg
    67501 attgccaaaa tggaaaaagg tgaaaggaat atcacgaatg gatttaagta ataaagcttc
    67561 aaatcttaga aaaaagttgg gagctgatgg tgaatcgccg atagatattt ttaaattggt
    67621 acaaaagata gaaaatttga cgctggtatt ttatggactc ggaaagaata ttagcggagt
    67681 ctgttataaa ggaactcagt tcagtctcat tgcagtcaat tcagacatgc cattaggaag
    67741 gtaaagattt tctttagcac atggactgta tcatctttat tatgatgagg tgaagaagag
    67801 ttcagtcagt cttatcttga ttggtgaagg agatgaaact gaaagaaaag cggatcagtt
    67861 tgcttcttat tttttaattt tcccatcttc actgtatagg atggttgagg aaatcagaga
    67921 aaatgccaat agaactcatc ttgaagtaga agatattata aaattgggtc agttttatgg
    67981 tatcagtcat aaagctatgt tatatagatt gaggaatgat ggataccttg atgcagaaga
    68041 aattaaaaat atggatatta gtgttataga gacagcttca agattaggct atgatacaag
    68101 tttatatcgt cctttgtcag aaagtaaaaa agaaatggca ttaggataat atattaattc
    68161 aactgaacaa cttttagaaa ataacagaat ttcgcaaggg aagtatgagg aactgttact
    68221 agatgctttc agatatgata ttgtatatgg gctagatgaa gaggggggag ttgtcgtttg
    68281 actagtcgtg tatttattga tgcagattgt atttcagtat ttttatgggt tggcactgaa
    68341 catcttttag aaaagctcta tttgggtaaa attgttattc cacaagaggt gtatgatgaa
    68401 atcaatatac ctacaattcc ccatttaaaa tctaggatag atcagttggt agctaagggt
    68461 tcagctgaga ttgtgagcat agacattgga actgaagaat acgcattata tagagattta
    68521 acaagaaatc atgatagtaa caagattatt ggtaaaggag aaggggcatc tatttcctta
    68581 gcgaaaaagc ataatgggat attaggaagt aataacctaa gagatgttaa accatatgta
    68641 gaagaatttt ctttagaaca tatgacaaca ggagatatac tgattgaagc gtttaaagcg
    68701 taatttatta ctgaacaaga gggcaatcat atctggaata atatgcttaa aaagagaagg
    68761 aaaattggtg caaattcatt cttcgtggaa gtattcatca aaatagacaa aaataaattt
    68821 ggataaatcg aactcactat tcaggaggca tatgagcaat tcgaaaaaga aaaatgtcaa
    68881 attgagccta taggagtaga agtgaaatag taagtcctgc atagtggatg agagaaaagt
    68941 tctccttgaa gttttcctga actatcagtc gcatgtcaaa cgatatgtag ggtaatgtga
    69001 gaggggatag cgagtagttt ttggttattt tatcaaaaaa cttatatttt attataccga
    69061 atgataaaat ataataaaaa tgatagaata aggaaaaaac atgaatgtca aaaagataat
    69121 gtcaattttt caatcctttt atgttgatgt cagtattgag gaactgactt tgactttacc
    69181 aatcagtttt gtaaaaaagt ttgagtatac tcaaatgact tttcataagg aatcattttt
    69241 attgattaaa gaaaagagaa gggggagttt gagttcattt gttactcagg ctcgcactat
    69301 gggtgataaa gccaatatgg atgttgtttt ggtgttttcg aagttatcag acggtgaaaa
    69361 aaagcaatta cttcaagcta gagttccgtt tgtagacttt aagggaaacc tcttcttccc
    69421 tacattggga ctagtactga atgcgaatga tactgaagtc cctaaggaat taatacctag
    69481 cgaacaatta acgtggattg cctttttatt gccagatggc gatataaagc agataaaatc
    69541 tgtttctaac cttctatatg gtggtgctta tgctttgtcg cattcaactt ttttagctga
    69601 aacggatgaa aatactagct atgtcatatg gcagagaaaa ttcaatcagt tatccttgcc
    69661 actttctcag catgttttaa aatgaaagat gctagagata tggaaatatc gtccttttgt
    69721 atctgagttt tggaatgatt ttaaaaataa tcatgataaa caatttgtag atccgatttc
    69781 tctttatttg accttaaaag atgatgatga cccacgtata gaggaagaga gtgaagcact
    69841 agaaaatatg atattacagt atctgggaga agatgatgcc agctaatacg aaagttattt
    69901 ttcaagaaat gtttgcggat tttcagaact attatgttct gattggggga actgctacct
    69961 ctatcgtatt ggattcgcaa ggatttaaaa gtcgcacaac aaaagattat gatatggtca
    70021 tcattgatga agtaaaaaat aaggaatttt atactacctt gaatcatttt ttagaattgg
    70081 gagagtatca aggaagtcag aaagatgaga aagcgcagct ttttcgattt acaacaacta
    70141 attctgagtt tccttctatg attgaactat ttagtatctt accagaatat ccattaaaga
    70201 aggacggtcg agaaattccc ttacattttg accaagatgc tagtttatca gccttattat
    70261 tggatgaaga ttattataat atattggtgc atgaaaaaga aaccattcag gggtattcgg
    70321 tattgagtaa ttgtggtc
//
LOCUS       DAVDWT010000006        68961 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVDWT010000006 DAVDWT010000000
VERSION     DAVDWT010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 68961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68961
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.006"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..383
                     /locus_tag="VUW50_000474"
     CDS             <1..383
                     /locus_tag="VUW50_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HET7982430.1"
                     /translation="VTGFYYGTPSENEQLFGARCKIPEYKFVAEVVSYDDATQTATIR
                     QRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELLTIKVPQLVQAGD
                     MVRALKEGLINLYKEDGTSVTVRA"
     gene            846..1611
                     /locus_tag="VUW50_000475"
                     /pseudo
     CDS             846..1611
                     /locus_tag="VUW50_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1627..2355
                     /locus_tag="VUW50_000476"
     CDS             1627..2355
                     /locus_tag="VUW50_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7982431.1"
                     /translation="MKLNKLNFLKENIRNLYSSGVIYLSLLISFIPPILLTFFILKTQ
                     GTSLGIKHISNFYAMLGMLMAVIHANRVISRDFSHNTVSLFYNQQKNRMIYVLSNFLY
                     AISVSIIYALNGIVLLVIVSKLGIPGDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYK
                     LKSGLVFGILVALLLFIPNILNTMMMNTSNDLFIKAIELLPFYSLPLFVASNTMSISQ
                     YLVLITTIILLYFFTLKKSKKYSF"
     gene            2740..2949
                     /locus_tag="VUW50_000477"
     CDS             2740..2949
                     /locus_tag="VUW50_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7982432.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            2939..3427
                     /locus_tag="VUW50_000478"
     CDS             2939..3427
                     /locus_tag="VUW50_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982433.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            3543..5003
                     /locus_tag="VUW50_000479"
     CDS             3543..5003
                     /locus_tag="VUW50_000479"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET7982434.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEGLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DENGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            5000..5824
                     /gene="nadE"
                     /locus_tag="VUW50_000480"
     CDS             5000..5824
                     /gene="nadE"
                     /locus_tag="VUW50_000480"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HET7982435.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGFHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            6223..6774
                     /locus_tag="VUW50_000481"
     CDS             6223..6774
                     /locus_tag="VUW50_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7982436.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDIEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            7489..8616
                     /locus_tag="VUW50_000482"
     CDS             7489..8616
                     /locus_tag="VUW50_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_135354309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET7982437.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDVDNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGYVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYNV
                     DGKSGQVTESTLSQKKDSQTRVVKKGTKPQVLVQEIPIETEYLDDPTLDKGQEVGEIG
                     KITLTTIYAVDERDGTIEETTSRQITKEMVKRRIRRGTREPEKVVVPEQSSIPSYPVS
                     VTSNQGTDVAVEPAKAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGS
                     MKVNQWFQVGGKWYYVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(9285..10313)
                     /gene="queA"
                     /locus_tag="VUW50_000483"
     CDS             complement(9285..10313)
                     /gene="queA"
                     /locus_tag="VUW50_000483"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET7982438.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDSMFIY"
     gene            10464..11171
                     /locus_tag="VUW50_000484"
     CDS             10464..11171
                     /locus_tag="VUW50_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HET7982439.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            11312..11488
                     /locus_tag="VUW50_000485"
     CDS             11312..11488
                     /locus_tag="VUW50_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET7982440.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            11618..12553
                     /locus_tag="VUW50_000486"
     CDS             11618..12553
                     /locus_tag="VUW50_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HET7982441.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            12546..13334
                     /locus_tag="VUW50_000487"
     CDS             12546..13334
                     /locus_tag="VUW50_000487"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET7982442.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            13335..13718
                     /locus_tag="VUW50_000488"
     CDS             13335..13718
                     /locus_tag="VUW50_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HET7982443.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            13734..14123
                     /locus_tag="VUW50_000489"
     CDS             13734..14123
                     /locus_tag="VUW50_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET7982444.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQVKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            14207..15337
                     /locus_tag="VUW50_000490"
     CDS             14207..15337
                     /locus_tag="VUW50_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HET7982445.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            15342..16079
                     /locus_tag="VUW50_000491"
     CDS             15342..16079
                     /locus_tag="VUW50_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HET7982446.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            16090..16863
                     /locus_tag="VUW50_000492"
     CDS             16090..16863
                     /locus_tag="VUW50_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HET7982447.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            16853..17470
                     /locus_tag="VUW50_000493"
     CDS             16853..17470
                     /locus_tag="VUW50_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HET7982448.1"
                     /translation="MKTKLIFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            17680..18081
                     /locus_tag="VUW50_000494"
     CDS             17680..18081
                     /locus_tag="VUW50_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HET7982449.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            18081..18359
                     /locus_tag="VUW50_000495"
     CDS             18081..18359
                     /locus_tag="VUW50_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HET7982450.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            18349..19122
                     /locus_tag="VUW50_000496"
     CDS             18349..19122
                     /locus_tag="VUW50_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET7982451.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRSVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            19123..20427
                     /locus_tag="VUW50_000497"
     CDS             19123..20427
                     /locus_tag="VUW50_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HET7982452.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKSSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            21001..21879
                     /gene="pstS"
                     /locus_tag="VUW50_000498"
     CDS             21001..21879
                     /gene="pstS"
                     /locus_tag="VUW50_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET7982453.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            21879..22796
                     /gene="pstC"
                     /locus_tag="VUW50_000499"
     CDS             21879..22796
                     /gene="pstC"
                     /locus_tag="VUW50_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET7982454.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            22786..23670
                     /gene="pstA"
                     /locus_tag="VUW50_000500"
     CDS             22786..23670
                     /gene="pstA"
                     /locus_tag="VUW50_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET7982455.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            23681..24484
                     /gene="pstB"
                     /locus_tag="VUW50_000501"
     CDS             23681..24484
                     /gene="pstB"
                     /locus_tag="VUW50_000501"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET7982456.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            24497..25255
                     /gene="pstB"
                     /locus_tag="VUW50_000502"
     CDS             24497..25255
                     /gene="pstB"
                     /locus_tag="VUW50_000502"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET7982457.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLNPQHKETEDYITGKFG"
     gene            25267..25920
                     /gene="phoU"
                     /locus_tag="VUW50_000503"
     CDS             25267..25920
                     /gene="phoU"
                     /locus_tag="VUW50_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET7982458.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            26077..26892
                     /locus_tag="VUW50_000504"
     CDS             26077..26892
                     /locus_tag="VUW50_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET7982459.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGIYQSYLEKAASLTEVEE"
     gene            complement(26912..28183)
                     /locus_tag="VUW50_000505"
     CDS             complement(26912..28183)
                     /locus_tag="VUW50_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET7982460.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNAIKEAFMAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            28614..29135
                     /locus_tag="VUW50_000506"
     CDS             28614..29135
                     /locus_tag="VUW50_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcin A-M57"
                     /protein_id="HET7982461.1"
                     /translation="MKFKKIVTSALVVLSLFSIMSPSIASVRVFADDVTTTEVSEIST
                     QEQKQIDDVANVLEQMFRNGVNEKNFTEYVYKNFSQKDIALAENELETNINNPYDRVP
                     WDEMGGCIAGKIRDEFFAMINVSLIVKYAQKKAWSELAKVVLRFVKANGLKTNIYIIA
                     GQLAIWAVQCGLE"
     gene            29619..30350
                     /gene="budA"
                     /locus_tag="VUW50_000507"
     CDS             29619..30350
                     /gene="budA"
                     /locus_tag="VUW50_000507"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HET7982462.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            30360..31112
                     /locus_tag="VUW50_000508"
     CDS             30360..31112
                     /locus_tag="VUW50_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HET7982463.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            31224..32129
                     /gene="murB"
                     /locus_tag="VUW50_000509"
     CDS             31224..32129
                     /gene="murB"
                     /locus_tag="VUW50_000509"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET7982464.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            32290..33447
                     /locus_tag="VUW50_000510"
     CDS             32290..33447
                     /locus_tag="VUW50_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982465.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEKRDEENKL"
     gene            33428..34234
                     /locus_tag="VUW50_000511"
     CDS             33428..34234
                     /locus_tag="VUW50_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7982466.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWIMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSAIGVILILTMFITMWVTKERRER"
     gene            34231..35004
                     /locus_tag="VUW50_000512"
     CDS             34231..35004
                     /locus_tag="VUW50_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7982467.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGNDMNSFTGFSLSH
                     FKTMFGDGRLMLIVTQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            35001..36071
                     /locus_tag="VUW50_000513"
     CDS             35001..36071
                     /locus_tag="VUW50_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7982468.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLEQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDLGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKSEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDPQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNAAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDA
                     AYAFINFMLKPENALKNAEYVGYSTPNLPAKELLPEETKEDKAFYPDAETMKHLEIYE
                     KFDHKWTRKYSDLFLQFKMYRK"
     gene            36325..36565
                     /locus_tag="VUW50_000514"
                     /pseudo
     CDS             36325..36565
                     /locus_tag="VUW50_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36840..37325
                     /locus_tag="VUW50_000515"
     CDS             36840..37325
                     /locus_tag="VUW50_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HET7982469.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            37347..39965
                     /gene="alaS"
                     /locus_tag="VUW50_000516"
     CDS             37347..39965
                     /gene="alaS"
                     /locus_tag="VUW50_000516"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET7982470.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVEVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINELFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVEAVSEGTASLIFAETSFYAEMGGQVA
                     DHGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(40055..41509)
                     /locus_tag="VUW50_000517"
     CDS             complement(40055..41509)
                     /locus_tag="VUW50_000517"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HET7982471.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            41687..42436
                     /locus_tag="VUW50_000518"
                     /pseudo
     CDS             41687..42436
                     /locus_tag="VUW50_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            42417..44051
                     /locus_tag="VUW50_000519"
     CDS             42417..44051
                     /locus_tag="VUW50_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982472.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEIPIYFSLMVAILLVIAFSTSLTAFYNVFYESK
                     DLVSYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLALYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSIEAILGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVVTVLFTSAFLPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFIALFIAVVNNNITSLPSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPILTLLGLSLYLGLPIISMIYLIVAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAISLGVGALILLLALAIFGYHYYLSRILAEIEKR"
     gene            44736..45899
                     /locus_tag="VUW50_000520"
     CDS             44736..45899
                     /locus_tag="VUW50_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET7982473.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDREVEVQGQEGN
                     FLGTAYLSQQNKGLGWFISKDKVTFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            45896..46573
                     /locus_tag="VUW50_000521"
     CDS             45896..46573
                     /locus_tag="VUW50_000521"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HET7982474.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            46563..47417
                     /locus_tag="VUW50_000522"
     CDS             46563..47417
                     /locus_tag="VUW50_000522"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET7982475.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            47436..48503
                     /locus_tag="VUW50_000523"
     CDS             47436..48503
                     /locus_tag="VUW50_000523"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET7982476.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            48513..49679
                     /gene="aroC"
                     /locus_tag="VUW50_000524"
     CDS             48513..49679
                     /gene="aroC"
                     /locus_tag="VUW50_000524"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET7982477.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            49689..50792
                     /locus_tag="VUW50_000525"
     CDS             49689..50792
                     /locus_tag="VUW50_000525"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HET7982478.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            50803..51141
                     /locus_tag="VUW50_000526"
     CDS             50803..51141
                     /locus_tag="VUW50_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HET7982479.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            51233..52516
                     /gene="aroA"
                     /locus_tag="VUW50_000527"
     CDS             51233..52516
                     /gene="aroA"
                     /locus_tag="VUW50_000527"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET7982480.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSSTLTVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            52509..52985
                     /locus_tag="VUW50_000528"
     CDS             52509..52985
                     /locus_tag="VUW50_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET7982481.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIVADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            52982..53830
                     /gene="pheA"
                     /locus_tag="VUW50_000529"
     CDS             52982..53830
                     /gene="pheA"
                     /locus_tag="VUW50_000529"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET7982482.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSIHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWLLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            53827..55101
                     /gene="psr"
                     /locus_tag="VUW50_000530"
     CDS             53827..55101
                     /gene="psr"
                     /locus_tag="VUW50_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HET7982483.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            55380..56225
                     /locus_tag="VUW50_000531"
     CDS             55380..56225
                     /locus_tag="VUW50_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET7982484.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSYKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKDPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTKMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDVVIADMDKSYTEYK
                     GEYGG"
     gene            56215..57318
                     /locus_tag="VUW50_000532"
     CDS             56215..57318
                     /locus_tag="VUW50_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7982485.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            57320..58306
                     /locus_tag="VUW50_000533"
     CDS             57320..58306
                     /locus_tag="VUW50_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7982486.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATDLSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNSIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            58322..59755
                     /locus_tag="VUW50_000534"
     CDS             58322..59755
                     /locus_tag="VUW50_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HET7982487.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGVLARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQAEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            59784..60818
                     /locus_tag="VUW50_000535"
     CDS             59784..60818
                     /locus_tag="VUW50_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET7982488.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEDSAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            61018..61755
                     /gene="mecA"
                     /locus_tag="VUW50_000536"
     CDS             61018..61755
                     /gene="mecA"
                     /locus_tag="VUW50_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HET7982489.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            61906..63192
                     /locus_tag="VUW50_000537"
     CDS             61906..63192
                     /locus_tag="VUW50_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET7982490.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKLTRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            63194..64063
                     /locus_tag="VUW50_000538"
     CDS             63194..64063
                     /locus_tag="VUW50_000538"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET7982491.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            64139..64216
                     /locus_tag="VUW50_000539"
                     /pseudo
     CDS             64139..64216
                     /locus_tag="VUW50_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            64358..65296
                     /gene="msrB"
                     /locus_tag="VUW50_000540"
     CDS             64358..65296
                     /gene="msrB"
                     /locus_tag="VUW50_000540"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET7982492.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            65354..67078
                     /locus_tag="VUW50_000541"
     CDS             65354..67078
                     /locus_tag="VUW50_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982493.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLLPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISTELTFWFL
                     VMVAILTIVIVGVSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFVEAPEDIHSELEQKQATRDKVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQKLWQALEIAQAK
                     DFVSEKEGLLDVLVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            67080..68828
                     /locus_tag="VUW50_000542"
     CDS             67080..68828
                     /locus_tag="VUW50_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982494.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTMDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
BASE COUNT        20089 a        12728 c        15495 g        20649 t
ORIGIN      
        1 tggttacagg attttactat ggtacaccat ctgaaaatga gcagttgttt ggtgctcgtt
       61 gtaaaattcc tgagtacaag tttgtcgctg aagtggtttc ttatgatgat gcgacacaaa
      121 cagcaaccat tcgtcaacgg aacgtcatta acgaagggga ccaagttgag ttttatggtc
      181 caggtttccg tcattttgaa acctatattg aagatttgca tgatgccaaa ggcaataaaa
      241 tcgaccgcgc tccaaatcca atggaactat tgactattaa agtcccacaa cttgttcaag
      301 ctggagacat ggttcgagct cttaaagagg ggcttatcaa tctttataag gaagatggaa
      361 ccagcgtcac agttcgtgct taatgtagtt gtttagtttt aaaaaattat gcaaagctcc
      421 atatacaaca cttaaacgag attaaagaat ggcgaaatcc cttgatgcgc aagagattag
      481 ctgtcctttt ttatttttta agtgataaag tcggagctta ggtattaaag cctatcaaat
      541 taaacaaaga agcgatgtct tagatatttt gaaaaaaatt aataagcaga aaactctcta
      601 ttattttgtt gtagagagtt ttttgttaat aaaattttac aaaatgacat ttatatattg
      661 cattaagtta gatatatgat ataatattgt taaaaagagg cgcaactttt taaactcaat
      721 aagaatcaaa gagcaaacca tgaatattaa tggaggaata aaaaatgtaa gttataaatg
      781 taagtaagca ttatggtcat tcaatcattc tcaaagatat aaattttgca cttaacaagg
      841 gtgaaattgt tggtctagta gggagaaatg gagttggtaa gagtacgttg atgaaaattc
      901 ttgttcaaaa taatcaaccg acttcaggta atattataag cagtgataat gttgggtatt
      961 taatcgaaga accaaaatta tttttatcta aaacaggttt agagaattta aaatatttgt
     1021 caaatttata tggtgttgac tacaatcaag aaagatttag aagtttgatc caagagttag
     1081 atttgactca gtctattaat aaaaaagtaa agacctattc tttgggtaca aaacaaaaat
     1141 tagctttgct tctaactctc gttacggaac ctgatatatt gattttagat gaaccgacta
     1201 atggtttaga tattgaatca tcacaaatag ttttagcggt tctaaaaaaa attagcttta
     1261 aatgaaaacg tgggaatttt aatatcgagt cataaattag aagacattga agaaatttgt
     1321 gagagagttc ttttcttgga gaacgggctt ttgacatttc aaaaagtagg aaaagatagt
     1381 cataatttct tgtttgagat agctttttca tcagctacag atagagacat tttcattacc
     1441 aaacaagaat ttggagatat tgttcaggaa gagggactga gaattactat gtctgggaat
     1501 attcaaagta gtgagctttt taaatttttt aacgaaaact ctattaaagt agttgatttt
     1561 gaaactaaaa aagagacgct taaagatatt tacctaaatc gttcaaaata aaggaaggtt
     1621 ataatcatga aattaaataa attgaatttt cttaaggaaa atataagaaa tttatattca
     1681 tcaggcgtaa tatatctcag tttgcttatc tcgtttatac cgccgatatt gcttacattc
     1741 tttattctaa agactcaagg gacatcgctt ggtattaagc atatttcaaa cttttatgct
     1801 atgctcggta tgttaatggc tgttatacat gctaatcgag tcattagtag agatttttcc
     1861 cacaatacgg taagtttgtt ttataatcaa cagaagaatc ggatgattta cgtcttgtct
     1921 aattttctat atgccatctc agtttccatt atttatgctt tgaatggcat tgtgttacta
     1981 gtcatagtaa gtaaattggg tattccaggt gatttaggat tagattttat agtagctatt
     2041 gtagtcaata caattttgtt agtcctgttt tattttctat tatcctacat tttctattta
     2101 tacaaattga aaagtggctt ggtatttggt attttagtag ctttactact ctttatccct
     2161 aatatattaa atacgatgat gatgaatact agtaatgatt tgtttatcaa agcaattgaa
     2221 cttcttcctt tttattcttt acctctattt gtggcttcaa atacgatgtc tattagtcag
     2281 tatcttgtgc taatcactac aatcatttta ttgtactttt tcactctcaa gaaaagcaag
     2341 aagtattcat tttagtttca tttagtatta ttttgcatac ctaaaataca gtaaaaaatc
     2401 agtcatcttg gtatgctcct gctttcacta ttcaacacgt ttttgactta tactaggctc
     2461 atttccaaaa gcattatata atagtgatat gaaaccaact aaactaaaca agaaatataa
     2521 gcaataaaaa ctcgtttaaa agatcctact aaagctaata ctaaataaaa ataaaagagt
     2581 aaactaggaa gtttatttca aacaacctaa aatactgatt ttcggctgaa gataatactg
     2641 gagtgcaaat taatggggtt ataataaata gctgatagct tgtgttggtt ttggattttt
     2701 taagagtaga tgagtattaa aactataagg aggacgaagg tggctaaaaa tttaaaatta
     2761 aaattagctc gggtagagcg tgatttaaca caaggtcaac tggcagaggc tgtcggggtg
     2821 acacgccaga ctattggttt gatagaggcg ggaaaataca atcccagtct ctcgctctgc
     2881 cagtctattt gcagatgttt agggaaaacc ctagaccaac tattttggga ggaagaagat
     2941 gaaaaataga ttttattatt atcaattact agacgaaaga gaagaacaac tgttcaataa
     3001 agcgggctct gaaagtttct atatctgcat cgctttgtcg ctcctatctt atatcatttc
     3061 agtattagca ccaagccttt ttaattctaa tatgctgcta atcgttatca tcatagggac
     3121 attttacttt ttcaatcgtg cccgttatct gggagtgacc tactatagtc gttttcattt
     3181 tacgattttg ggttgttttt tcctaacctt ggctattacg gctcttttga tgttgcagaa
     3241 ttatcaattc aacatagaaa tttatcagca caatcctttg aattttaaat acctgtctgc
     3301 ttgggtcatt acttatgtca tttaccttcc gtgggtcttt attggcaatc ttggtcttaa
     3361 gagctatggc gaatgggctc agaaaaaatt tgaacaagat atggatgaat tggagagtgg
     3421 agaatagctt gttactcttt tctcaatcca gctaaaatgt gatataatag tactaattta
     3481 ttggaataca tgaaagttct tgaaaatttt catgggtttc tagctaagga agtaggaaaa
     3541 gtatgtatcc agatgatagt ttgacattgc acacggactt gtaccagatc aacatgatgc
     3601 aggtttactt tgaccaaggg attcacaata agaaggcggt ctttgaggtg tatttccgcc
     3661 aacagccttt taagaacggc tatgcggttt ttgcaggttt ggaaagaatt gtgaactatc
     3721 ttgaaggctt gcgtttttca gatagtgata tagcctattt ggagtcgctt ggttatcatg
     3781 gggcgttctt ggattacctt cgcaatttca agttggagtt gaccgttcgt tctgcccaag
     3841 aaggggattt ggtttttgct aatgaaccga ttgtgcaggt ggaaggacct ctagcccaat
     3901 gtcagttggt cgaaacggct cttttgaaca tcgtcaacta ccagaccttg gtggcgacga
     3961 aggcagctcg tattcgttcg gttatcgaag atgaaccctt gatggagttt gggacacgtc
     4021 gggctcaaga aatggatgcg gccatctggg gaacacgcgc agcggtgatt ggtggcgcca
     4081 atggaaccag caacgtgcgt gcgggtaagc tctttgacat tcctgttttg ggaacccatg
     4141 cccatgcctt ggtacaggtt tatggcaatg actatgaggc tttcaaggct tacgctgcga
     4201 cccacaaaaa ttgtgtcttt cttgtggata cctatgatac ccttcgcatc ggtgtaccag
     4261 ctgccattca ggtggcgcgt gagctgggtg atcagattaa ctttatgggt gtgcggattg
     4321 actctgggga tattgcctac atttctaaga aagtccgtca gcaactggac gaggctggat
     4381 ttacagaggc taagatttat gcttctaatg atctagatga aaataccatc cttaacctca
     4441 agatgcaaaa ggccaagatt gatgtctggg gtgtgggtac caagctgatt acagcctatg
     4501 accagccggc tcttggggcg gtttacaaga ttgttgcaat cgaagatgaa aacggccaga
     4561 tgcgcaatac gattaagctg tctaataatg cggaaaaagt gtcgacgcca ggtaagaagc
     4621 aggtgtggcg cattaccagt cgtgaaaaag gcaagtcaga aggtgactac atcacttatg
     4681 atggtgtgga tattagcgac atgacagaaa tcaagatgtt ccatccgacc tatacataca
     4741 tcaagaagac ggttcgtaat tttgatgctg ttcctctctt ggtggatatc ttcaaagaag
     4801 gaatattagt ttacaacttg cctagtttga ctgacattca ggattatgcc cgtaaggaat
     4861 ttgacaagtt gtgggatgag tataagcgtg tgctcaatcc gcagcactat ccagtggatt
     4921 tggcgcgtga cgtatggcaa gataagatgg acttgattga taagatgcgc aaggaagccc
     4981 ttggtgaagg agaagaagaa tgagtttgca agaaacgatt atccaagagc tgggtgtcaa
     5041 accagtgatt gatgcccagg aagaaatccg tcgttctatt gatttcttaa aaagatatct
     5101 gaaaaaacat cccttcctaa aaacctttgt actagggatt tctgggggac aagactcaac
     5161 cttggcagga cgtttggcgc aattagctat ggaagaactg cgagctgaaa cgggagacga
     5221 tagctacaaa tttatcgctg tccgcctgcc atacggagtg caagctgatg aagcagatgc
     5281 tcaaaaagcc ctagccttca tccagccaga tgtcagcttg gttgtgaata tcaaggaatc
     5341 agctgatgcc atgacagctg cagttgaagc gacaggtagt cctgtttcag acttcaacaa
     5401 ggggaatatc aaggcacgtt gccgtatgat tgctcagtat gcccttgctg gtttccatag
     5461 cggagcggtc attggaacag accacgccgc agaaaatatc acaggtttct ttaccaagtt
     5521 tggtgacggc ggtgcggata ttctccctct ttaccgcctc aataaacgcc aaggaaaaca
     5581 gctcttgcag aaacttggcg cagagccagc cctttatgaa aaaatcccaa cggcagacct
     5641 agaagaagat aaaccaggcc tagctgacga agtcgcactt ggagtcacct acgcagagat
     5701 tgacgactac ctagaaggca aaacaatcag cccagaagct caagcgacca ttgaaaactg
     5761 gtggcacaaa ggccaacaca aacgccactt acccatcact gtatttgatg acttttggga
     5821 gtaaaaacct ccactggagg tttttaccct gagcctagaa atgagaaagc gaagaaggtc
     5881 cgggggacct ttttagcttc ttgccctgaa attaaaaagc aagaaaaacc tccagtggag
     5941 gttttcagcc tctcatcttg aaataagaaa gtgagagaag gtctggggga tcttgaaccc
     6001 cgagtttaga aataagaaaa tgaggcagat tcggtaactc gaagagttcg atttcatcgt
     6061 cttacccctg caacgatgac taggtttgaa aaagcttgct agagcgcatt tcaaaccagg
     6121 cagcgactgc gtcaagaaat tagaagacaa actcgttttc tagctgttac tgagttgagc
     6181 ctttttacta cgagcataga aataaggaag tgaggtagca tcatgaaatc tatcggtacg
     6241 caaatattac agacagaacg tttgatttta agaagatttg tggagagtga tgcagaagcc
     6301 atgtttcaaa attgggcttc atccgctgag aatctgacct atgttacctg ggatccccat
     6361 cctgatatcg aaatcactcg aaactcgatt cgcaattggg ttgcttccta tactaatctc
     6421 aactattata aatgggccat ttgtctaaaa gaaaacccag agcaagtgat aggagatatc
     6481 agcattgtta agatagacga ggctgattta agctgtgaaa ttggctatgt gttaggcaag
     6541 gcttactggg gaaatggtat gatgacagag gccttgaaag ctgtcttgga cttttgtttt
     6601 actcaagcag gttttcaaaa ggtcagagca cgttatgcca gtctcaaccc agcttcaggt
     6661 cgtgtcatgg aaaaggctgg aatgtcctat ctacaaacca ttgttaatgg tgtagagaga
     6721 aaaggctatc ttgcggatct tatttattat ggtataagta gggaagaatg ttgaattcta
     6781 ttttctgttt ctatcgaagt caactattta ttgtaaatat aataattagc attccaagtt
     6841 tatttgcaac tttaaaatag catattgatt agtacaagac agatgttcta gttccttctt
     6901 taatctggtt tagtattagt taaaaaatcg cttgaagctt gtaactaaga gggagctaat
     6961 cgactagatt ctccagccga acaggtggta atgtgctttt tatagtgtaa tcctagctgt
     7021 tgttaaattt aaaatagaat cctctatcga gttagggaat taaattcaac caattttatt
     7081 tatgtttttt ctatcaaaat atctaatatt aaaatagtct cattctgatg agaaaactat
     7141 tctatttata ctgcatagtt gtataaggaa tgatgaaaga tgagtgaaag ttatcaagtt
     7201 ggaatgtttg tatctaaata tattagcatg tatttagata agatgtctgc aatcctttat
     7261 atatgagatt gattacgata tagttaaagt gttagttcaa taatcaatat atcattctaa
     7321 gtctatttta tatataaagt atattgttta atgtttgata gaaaagatgc tatactagaa
     7381 gtaggatgta atggcaaaat ctaatactaa taagcgctat gcaatacgta aactcggaaa
     7441 gatttttggt tctgcggtaa tcgggctaac tttattttta ggaggaatct gaatacaagt
     7501 tttgttcatg ctgctgatgg gattcaatat gtcagagatg atactagaga taaagaagag
     7561 ggaatagagt atgatgacgt tgacaatggg gatattattg taaaagtagc gactaaacct
     7621 aaggtagtaa ccaagaaaat ttcaagtacg cgaattcgtt atgaaaaaga tgaaacaaaa
     7681 gaccgtagtg aaaatcctgt tacaattgat ggagaggatg gctatgtaac tacgacaagg
     7741 acctacgatg ttaatccaga gactggttat gttaccgaac aggttactgt tgatagaaaa
     7801 gaagccacgg atacagttat caaagttcca gctaaaagca aggttgaaga agttcttgtt
     7861 ccatttgcta ctaaatatga agcagacaat gacctttctg caggacagga gcaagagatt
     7921 actctaggaa agaatgggaa aacagttaca acgataactt ataatgtaga tggaaagagt
     7981 ggacaagtaa ctgagagtac tttaagtcaa aaaaaagact ctcaaacaag agttgttaaa
     8041 aaaggaacca agccccaagt tcttgtccaa gaaattccaa tcgaaacaga atatctcgat
     8101 gacccaactc ttgataaagg tcaagaagta ggagaaattg gtaaaattac tcttactaca
     8161 atctatgctg tagatgaacg tgatggaaca attgaagaaa ctacttctcg tcaaattact
     8221 aaagagatgg taaaaagacg tataaggaga gggacgagag aacctgaaaa agttgttgtt
     8281 cctgagcaat catctattcc ttcgtatcct gtatctgtta catctaacca aggaacagat
     8341 gtagcagtag aaccagctaa agcagttgct ccaacaacag gctggaaaca agaaaatggt
     8401 atgtggtatt tttataatac tgatggttcc atggcaacag gttgggtaca agttaatggt
     8461 tcatggtact acctcaacag caacggttct atgaaagtta atcaatggtt ccaagttggt
     8521 ggtaaatggt attatgtaaa tgcatcgggt gagttagcgg tcaatacaag tatagatggc
     8581 tatagagtca atgataatgg tgaatgggtg cgttaacaga ttcctccctg agacaaaatt
     8641 gataatagta tgtctgaata tataattata tataaaaagc tatagataag gtcattttat
     8701 agcgactata aaaaagtgaa caagcctagt cttctgtaca ttagaattca gatttgttca
     8761 ctttttgttt aatactcaat gaaaatcaaa gagcaaacta gaaaactaga aaactagtcg
     8821 caggttgctc aaagtactgc tttgaggttg tagatagaac tgacgaagtc agtcacatat
     8881 ataatccaag gtgacgttga cgcggtttga agagattttt gaagagtata agtctttgat
     8941 tttgagacta aggagagtca gggtcaaagt agtgaatgat tctgaaataa ctcataatag
     9001 tctcttgaga ggacagataa gagtaacaaa tatggtgcta ctcatgtgtc ttgcattttg
     9061 atttacaaac agcgaagaaa atataggaag ctgaaataaa atatagatga atcaatttgg
     9121 agagtcaacg caatctatgg caatattttc gaagctaggg ggaagcattt taatttcaat
     9181 gtactaggcg tcttctacag ctagctagag tttcaattgt agatgttctt tgaaataata
     9241 tcttagcgat ttattggtat tagaagattt agagaaattc tttctcaata aataaacatg
     9301 gagtcaccaa aactgaagaa gcggtagtgt tcttggatgg catggtggta ggcatctaag
     9361 actaattcac ggcctgcaaa ggcagaaacc aacatgacca gagttgattt tggcagatgg
     9421 aagttggttg agaaggcatc cacgaccttc cactcatacc caggtttgat aaagatattg
     9481 gtccaaccag aatctgcttg gatttgccca tcaaacttgg aaccaatagt ttccaaggtg
     9541 cggatagaag tggttccgac agcgatgaca cgaccaccat tttttttgac agagcgaagg
     9601 gtggcagcag cttcctcaga aagttgatag aactctgagt gcatttcgtg ttcgtccaga
     9661 ttatccacag aaacaggtct aaaggttccg agtccgacat ggagagtcag atagactaga
     9721 tgaacaccct tagcttggat ttctgccagc agttctttgg tgaagtgaag accagcagtc
     9781 ggtgctgcag cagagccgct ttccttggcg tagacggttt gataacgttc acggtcatct
     9841 aatttttcgt ggatataagg tggcagaggc atttctccca gactttccaa gacttctagg
     9901 aaaattcctt ggtattcaaa gcggacaatg cgtcccccgt gggtcaattc ttctgtaacg
     9961 acagcgctga ggcggccatc accaaagctg atacgagtac cgaccttgag gcgtttggca
    10021 ggtttagcca gaacttccca ctcgtctcca ctagtgttct taaggaggag aagttccaca
    10081 tgacctcctg tctccacttt ttgaccatag aggcgggcag ggagaactcg ggtgtcgttc
    10141 atgacaaggg catcaccagg ttccagcata tcaataatag agtggaagtg tttatcttgc
    10201 atttctcctg tctcacggtt gacgatgagg agtttggagg catcacgttt ttcaaggggc
    10261 gtttgggcaa tcaattcctc tggcaagtgg aaatcaaaat cagctgtatt catatatctg
    10321 ttcattcttt ctaagttcta atcctatcca ttataacacg tttcaagttt ggacgctctt
    10381 tttttagaaa ttaaatcgtt ttcacttgac aaaaattggt ctataccata taataaatat
    10441 agacagaaat ggaggatgaa aagatgaaag ttattaaagt tgaaaaccaa gttcaaggtg
    10501 gaaaagttgc ttttgagatt ttgaaggaaa aattggctaa tggagctcaa actctaggac
    10561 ttgcgacagg aagtagccca cttgaatttt acaaggaaat tgttgagagt gaccttgatt
    10621 tttcaaatct aaccagtgtc aaccttgatg agtatgtggg ccttgatggg gacaatccac
    10681 agtcttatcg ttacttcatg caagaaaact tgttcaacca aaaaccattt aaagaaagtt
    10741 tcttgcctcg tggtgttaag gacaatgctg aagctgaagt tgaacgctac aaccaaattt
    10801 tggctgacca tccagttgac ctccaaatct tgggaatcgg tcgcaatggg catatcggct
    10861 ttaatgagcc tggtactcca tttgacagtc aaacgcatct agtagaactt gaccagtcta
    10921 ctatcgaagc taatgcacgc ttctttgcta agattgaaga cgtcccaacc caagctattt
    10981 caatggggat taaaaacatt ttggatgcca agtctattat tctttttgct tacggtgagt
    11041 cgaaagcaga agccattgcc ggaacagtat ctggcccagt gactgagaac ctacctgcaa
    11101 gtagcctcca aaatcaccct gatgtaacca tcatcgcaga tgctgaagcg cttagcttac
    11161 tcgaaaaata aaaaagcaat gttctattga acgctttcaa ctcttgtata aatggaagaa
    11221 aaaaatcaaa attaaaccgc atttttgctt gacaattatt ccttttacgt gtagaatgaa
    11281 atagatattg aacttgaagg gagtgaaaaa aatgtctaaa acagtagtac gtaagaatga
    11341 atctcttgac gacgcacttc gccgtttcaa acgtgcggtt actaaagctg gtactcttca
    11401 agaaacacgc aaacgtgaat tctatgaaaa accttctgta aaacgtaaac gtaaatcaga
    11461 agtagctcgt aaacgtaaaa aattctaatt gaaatgaaag gctagagaaa tctagtcctt
    11521 tttcttttaa ataaatactc caaagcctgc aaaaatctga aacttcctcc tacaatttga
    11581 tataatagag agaagaactc atttgaagga ggaaatgatg tcggttttag taaaagaagt
    11641 gattgaaaag cttagactag acattgtcta tggtgaacca gaattgcttg aaaaggaaat
    11701 caatacagcg gatattacgc gacctggtct tgaaatgaca ggctattttg actactatac
    11761 accagagcgg attcaacttt tggggatgaa ggagtggtct tatctgatca gcatgccttc
    11821 caacagccgt tatgaagttt tgaaaaaaat gtttctacct gagacaccag cagtcattgt
    11881 tgcccgtggt ttggtggttc cagaggagat gttaaaggct gctagagaat gtaagattgc
    11941 tattttaacc agccgtgcag ctaccagtcg tttatctgga gagttatcta gctatctgga
    12001 ttctcgtttg gcagaacgta ccagtgtgca cggtgtcttg atggatattt atgggatggg
    12061 cgtcttgatt cagggagata gtggaattgg taagagcgag acaggtcttg agcttgtcaa
    12121 acgtggtcac cgtttggtag ccgatgaccg tgtcgatatc tttgccaagg atgagattac
    12181 tctctggggt gaaccagctg aaattttgaa acacttgatt gaaattcgtg gggttggtat
    12241 tatcgatgtt atgagtctct acggtgcgag tgctgtcaag gattcttcac aggttcagct
    12301 tgctgtctat ttggaaaatt acgatacgca taagaccttt gatcgtcttg gaaacaatgc
    12361 agaggaactt gaagtttctg gcgtagccat tcctcgtatt cgtattccag ttaaaacagg
    12421 tcgtaatatc tctgttgtga ttgaggcagc tgccatgaat tatcgtgcca aggaaatggg
    12481 ctttgatgcg acccgtttgt tcgacgaacg actgacaagt ctcatcgctc gaaatgaggt
    12541 gcaaaatgct tgatccaatt gctattcaac taggacccct agccattcgt tggtatgcct
    12601 tatgtattgt gacaggcttg attcttgcgg tttatttgac catgaaagaa gcacctagaa
    12661 agaagatcat accagacgat attttagatt ttatcttagt agcctttccc ttggctattt
    12721 taggagctcg tctctactat gttattttcc gatttgatta ctatagtcag aatttaggag
    12781 agatttttgc catttggaat ggtggtttgg ccatttacgg tggtttgata actggggctc
    12841 ttgtgctcta tatctttgct gaccgtaaac tcatcaatac ttgggacttt ctagatattg
    12901 cggcgcctag cgttatgatt gctcaaagtt tggggcgttg gggtaatttc tttaaccaag
    12961 aagcttatgg tgcaacagtg gataatctgg attatctacc tggctttatc cgtgaccaga
    13021 tgtatattga ggggagctac cgtcaaccga ctttccttta tgagtctcta tggaatctgc
    13081 ttggctttgc cttgattctg atttttagac ggaaatggaa gagtctcaga cgaggtcata
    13141 tcacggcctt ttacttgatt tggtatggtt tcggtcgtat ggtcatcgaa ggtatgcgaa
    13201 cagatagtct catgttcttc ggacttcgag tgtcccaatg gctgtcagtt gtccttatcg
    13261 gtctcggtat aatgatcgtt atttatcaaa atcgaaagaa ggccccttac tatattacag
    13321 aggaggaaaa ctaaatgtta gaagttgcat atattcttgt tgccctagct ttgattgtct
    13381 ttttggtcta tctgatcatt actgtacaaa agcttggtcg tgtcatcgat gaaacagaaa
    13441 agacgattaa aaccttgact tcagatgtgg atgtgacctt gcatcacacc aatgagttgt
    13501 tggctaaggt caatgtcttg gcagatgata tcaatgtcaa ggtggctacg attgatccac
    13561 tcttcagtgc tgttgcagat ttatctctat ctgtttcaga cctcaatgac catgcgcgtg
    13621 tcttgagcaa gaaagcttca tcagctggtt caaaaacact caagactggt gcaagtctgt
    13681 cagctcttcg tcttgcaagt aaatttttca aaaaataaaa aaggagaatc cttatgggta
    13741 aattatcctc aatcctttta ggaacggttt caggtgcagc tcttgccttg tttttaacaa
    13801 gtgataaggg caaacaagtt tgcagtcagg ctcaagattt tctagatgat ttgagagaag
    13861 atccggagta tgccaaggag caagtctgtg aaaaactgac agaagttaag gagcaggcta
    13921 cagattttgt tctgaaaaca aaagaacagg ttgagtcagg tgaaatcact gtggacagta
    13981 tacttgctca agttaaatcc tatgcttttc aagcgacaga agcatcaaaa aatcaattaa
    14041 ataatctcaa ggaacaatgg caagaaaaag ccgaagctct tgatgactca gaagagattg
    14101 tgattgatat aacagaagaa taaaccatca ccatctccgg acggactatg tatctgggga
    14161 tggtgatttt tatctggaat ctagtctttg tggtataata attactatgc agaaaaaacc
    14221 aacgtcagcc tatgtgcaca tcccattttg tacccagatt tgttattatt gtgatttttc
    14281 aaaggtcttc atcaaaaatc agccagtcga cagctattta gagcatctgc tggaagagtt
    14341 tcgttcttat gatattgaaa agttgtcaac cctttatatc ggtggcggaa caccgacagc
    14401 cctgtcggct ccgcaactgg aggttttact gaatggcttg actaaaaatt tggacttgtc
    14461 tgtcttagaa gagttgacca ttgaagccaa tccaggcgat ttggatgcgg ataagatagc
    14521 tgttttgaaa aattcggctg tcaatcgtgt ttcgctaggt gtccagacct ttgatgataa
    14581 gatgttgaaa aagattgggc gcagtcattt ggagaaggat atttatgaaa atatcgatcg
    14641 cctgaaactg gctggttttg acaacatctc cattgatttg atttatgctc tgcctggtca
    14701 gaccatggag caagtaaagg aaaatgtggc taaagccatt ggactggata ttccccacat
    14761 gagtttgtat agtttgattt tagaaaacca tacggtcttt atgaaccgga tgcgacgtgg
    14821 gaaattgcct ctgcctaagg aggaactaga agcggagatg tttgagtaca tcattgcaga
    14881 gctggagcga gcgggttttg agcattatga gatttctaat ttctccaaac ccggctttga
    14941 aagtcgtcat aatctcatgt actgggacaa tgctgaatac tatggtattg gtgcaggggc
    15001 atctggttat gtcaatggag tacgttataa aaatcatggt cccattcgtc attatcttag
    15061 tgcggttgag gaaggcaatg cttgtattac agaagatcac ctgagtcaaa aggagcaaat
    15121 ggaagaagaa atgttcttgg gactccgcaa gaaatccggg gtttccatgg cgcgatttga
    15181 ggaaaaattt ggacagtctt ttgctggact ttatggagaa attgtcagag atttggttca
    15241 acaaggcctc atgcagattg agggtgacca cgttcgcatg acaaagagag gtctcttttt
    15301 gggagatact gtagcagaac gatttatttt ggagtaggat catgggctta acttatcaaa
    15361 tgaaaatgaa aattcctttt gatatggctg atatgaacgg tcatatcaaa cttccagatg
    15421 tgattttgct gtcactgcaa gtttcaggga tgcagtcgat tgaactggga gttagtgata
    15481 aggccatttt ggaagactat aatctggtct ggattatcgc agaatatgat attgaggtgg
    15541 ttcgtttgcc tcgttttgcg gaagaaatta ccatcgaaac ggaagccttg agctacaatc
    15601 gacttttttg ctaccgtcgc tttactattt ataatgaagc ggggcaggag ctcatccaca
    15661 tgatggcgac ctttgttctc atggaccgcg acagtcgaaa agtccatgtt gttgaacctg
    15721 agattgtggc tccttaccag tctgattttg ataaaaaact tatccgtgga ccgaagtatg
    15781 aatccttgga agagccgatc agcaaggatt accatgttcg tttttacgac ttggatatga
    15841 atggtcatgt caataacagt aaatacttgg actggatttt tgaggtcatg ggagcggatt
    15901 ttttgaccca ctatattccc aagaaaatca acctcaagta tgtcaaggaa gttcgaccag
    15961 gtggggtgat tacatcggct gttgaacgga ctggactgga aagcaagcat gaaattacaa
    16021 gtgatggggt tatcaatgcc caagcaatca ttacttggca agaaataaaa aaggcttagg
    16081 gagaaataga tgaaatataa aggctattta attgatttag acggaaccat ttataagggg
    16141 aaagaccgaa tccctgctgg agagactttt gttcatgaat tgcaaaagcg ggacattccc
    16201 tatctctttg taaccaacaa tacaacccgc actccagaga gtgttaagga gatgttggct
    16261 cagaatttta atatcgatac gcccctatcg actgtctaca cagcgacttt ggcaaccatc
    16321 gactatatga atgatttggg gcttgaaaag accgtctatg tcgtcggaga agcaggactc
    16381 aaggaagcca tcaaggcggc tggttatgtg gaagacaagg aaaaccctgc ctacgtggta
    16441 gttggtctgg attggcaagt cgactatgaa aaatttgcca cagcaactct tgctattcaa
    16501 aagggtgccc attttatcgg aaccaatcct gacctcaaca tcccgacgga acgcggtctt
    16561 ttgccaggtg ctggctcact gattacgctg cttgaagtag cgacacgagt gaagccggtt
    16621 tatatcggaa aaccaaatgc catcattatg gacaaggcgg ttgagcactt aggtttggaa
    16681 cgtgaagagt taatcatggt tggggacaat tatttgactg atatccgggc tgggattgac
    16741 aatggcattc caacgctctt ggtgacgaca ggttttacta aggcagaaga agtagctggc
    16801 ctgccaatcg caccgacaca tgtggtttct agccttgcgg agtgggattt tgatgaaaac
    16861 taaattgatc ttttggggct ctatgctctt tctcctctcc ctctcaatcc ttctgaccat
    16921 ttatctggct tggattttct atcctatgga gattcagtgg ctaaacttaa cgaatcgagt
    16981 ctatctaaaa ccagaaacca ttcaatacaa ttttcatatc ttgatgaatt atctgaccaa
    17041 tccttttagt caggtcttac agatgcctga ttttcgttcg tcagcagctg gtctgcacca
    17101 tttcgcagtg gtcaagaatc tctttcattt ggttcagcta gtagctctag tgacactgcc
    17161 aagtttctat gtctttgtca ataggattgt gaaaaaggac tttttgtctc tttatcgaaa
    17221 aagtctcctg actctagtag tcttacctgt gatgattgga cttgggggag ttttgattgg
    17281 ttttgaccaa ttctttactc ttttccatca aattctcttt gtgggagatg atacctggct
    17341 ttttgatcca gccaaggatc ctgttattat gattttgcca gagaccttct ttcttcatgc
    17401 cttcctcctc ttttttgccc tctatgaaag cttctttggg tatctgtgtc tgaaaagtcg
    17461 taggaagtga aatgaaagat gtttttaatt tgcatatata aaaatatata tcgtttaaaa
    17521 ccatcgttaa gagtgaatta accaaatcca attgtgtcaa tcgttcgcaa acagcctatt
    17581 tttcttgaaa aaataggctt tttttctaaa aatccctagt caagcgcctt atttttttgt
    17641 ataatagaag tagcaaagta tagataagaa gagaaaagca tgattacact atttctatca
    17701 ccgagctgta catcatgtcg taaggctaag gcttggttag aaaaacataa ggttcccttt
    17761 gtggaacaca atattatgac cagtccttta acaagaaaag aattgcaaca catcctttcc
    17821 ttgaccgaaa atggtactga tgacatcatt tcaactcgtt caaaaatttt tcaaaaattg
    17881 aatattgatg tagagagtat ttcggtatcg gaattgcttc atttgattga gcagtatcct
    17941 agtcttttgc gtcgtccaat tattattgat gccaagcgta tgcaaatcgg ttttaatgaa
    18001 gatgagattc gtgcttttct ccctcgtagt tatcgtaagc aagaactaaa agaagcaaga
    18061 atgagagctg gtattagtta atgaacaaac agtatagtta cccactagat ttgtcgtgga
    18121 gcactgaaga acttgcttca gtgctttctt tttttaatga tgttgaagct gcctatgaag
    18181 gcaaagtaga ggctaaaaag ttactggact cctataaggg attcaaagcg gtcgtgccaa
    18241 gcaagagcga agagaaacgt ttgggacgag aatttgaaac ggttagtggc tattcacttt
    18301 accgagcagt tcaggctgcc aaagaaaaag gggaaggaaa gatttctctt ggaaagtaaa
    18361 tttgaatttg ctaaagagct tgttaagaaa gcaggtcagt acattcttga ccatatgcag
    18421 gaagacttgc gtgttgaaac caagtcctct ccaacagatt tggtgaccag actggacaaa
    18481 gaagttcagg aactcttggt tggtgagatt ttgtcccgtt atcctgagga taagatttgt
    18541 gctgaagaag gttgtctgcg agcctcggtt caagagggca aggtttgggt cattgatccc
    18601 attgatggta ccaataattt tgtagcccag caggaagatt ttgctgttat gatggcttat
    18661 tttgaaaatg gtcagggaca gtttggtctg atttatgatg tggtcaaagg ggattgttac
    18721 cacggtggtg gggaatttcc agtttgtcta aatgataggc ccctagctcc ttttaaaact
    18781 aaaccacttg gagattttct cattgcaggg aatagtggta tgctggaaac caatgaatgg
    18841 gggctggctg atttgagccg atcggttctt ggtgttcgtg tctatggaag tgcggccatt
    18901 agttttgcca agattttgtc agggcgtttg ttgacctatc tcacttatct gcaaccatgg
    18961 gattatgctg cagctagtat tttaggggaa agtctgggct atcgggttgt gacccttttt
    19021 ggtgaagctc ctgattttca aaccagacag ccggtcatga tggtgcctct tgagatgcag
    19081 gaggaaattc agtcctatat ttacgaaagg aaaagaactt aaatgcaatt tccagaagga
    19141 tttgttgaaa aatataaaga gatactagga gatgaggcaa gagattttct tgcctctttt
    19201 gaggaggaag cggtttcggc ctttcgggtc aatcccttaa aagaagagca actttccttt
    19261 tctgatgcca ttactcaaac cccttggggc cactatggga aggtttcagg gaaatcgcct
    19321 gagcatgcta caggtttagt ttattcgcaa gaacctgctg ctcaaatggt ggctcaagta
    19381 gcccaaccca gtcctggtat gaaggtcttg gacttggctg ctgcaccggg gggcaaatca
    19441 actcaactgg cagcctatct agcaggggag gggctacttg tttccaatga aatttcaagc
    19501 aaacgggcta agattttggt tgaaaacatg gagcgttttg gagcgacaaa tgtcgtggtg
    19561 accaatgaat ctgccgaccg cttggtcaag gtctttaaag gctattttga cttaatcgtt
    19621 cttgatgccc cttgctctgg tgaaggaatg tttcgtaagc aacctgatgc tatggactat
    19681 tggagcttag attatccgag tcaatgtgct agcttgcaaa gagaaattct ggaggatgca
    19741 gtgaccatgt tagctgaagg tggtcgtctg gtttattcga cctgtacctg ggcacccgag
    19801 gaaaacgaag agattgtcaa ttggttgctg gaagagtatg attttgactt gttgccagta
    19861 gagcatatca atggaatggt agctggtatt gacctgccag aaacggctcg gatgtaccct
    19921 catcagttta agggagaggg tcagtttgtt gcccatctac agtttaaggg taacaatcca
    19981 gctcctaaat ttaaggcaag taagagtaac ctcagccgtg aacaagttgc cttgtggcag
    20041 gaatttgccc aaaaccattt gaaggtcaat ctaccgggta tcttgcagac ttttggagac
    20101 cagctttatc tcttgccaga acttttgcca gatttaggga agctcaagat tgctcgcaat
    20161 ggactgcatc tgggtacttt taagaagaaa cgctttgagc ccagtttcgc tcttggttta
    20221 gccttgaaat cgagtcaggt cgaacagtcg gttgaaattg gccaagaagc gtttgtcaag
    20281 tatgcggctg gagaaatcgt tcagctggca gaaagtctgc caaatggttg gtaccaagtt
    20341 ttggttaaag gcaatggctt gggctttgca aaggttactg gaaatgtttt gaaaaattat
    20401 tttccaaaag gcctcagatt caaatgaaaa tgctagtcaa atatcttatt ctatgttata
    20461 gtgaaagtat ggattttttt agtaggatga ttcttgatat cttgattgcc attacatgta
    20521 gttttactac tcaatgaaaa tcaaagagca aactaggaag ctagccatag atagctcaaa
    20581 acactggttt gaagagattt tcaaagagta caagaagttt agttattagc gttcttaccg
    20641 cttgtaaact agatttctca taaaatagaa tcttttcctt ttagttgtaa actagtctgg
    20701 gagagtagag aggtttgaga tacctttcta gcttttggat tatcatctaa ggagagtaat
    20761 ttcccttgca ttaaaaaggg gaaaaagaga cacgaaatga ctataatggg tgacaatggg
    20821 ggaagggata gacaagagat tttatccaca tatgaaaaaa ggaggttagg aaagagtaat
    20881 atagcctata ttatataaat aatcaattgc gcagaaattt ggtaagaatt catgcgtcaa
    20941 ctcataaaga actacttaaa aaattcacag tattcataat tattttcgag gagaaaaaca
    21001 gtgaaaaaaa gaaaaaagct tgctctgtct cttatcgctt tttggctgac ggcttgttta
    21061 gtaggctgtg ctagctggat tgatcgtgga gaatccataa cggctgttgg ctcaactgcc
    21121 ttgcaaccct tggttgaagt agcggcagat gaatttggca cgatccatgt tggaaaaacg
    21181 gtcaatgtcc aagggggagg ttctggtaca ggcttgtccc aggttcagtc tggggcagtt
    21241 gatataggaa actcagatgt atttgctgag gaaaaagacg gaattgatgc ttctgctctt
    21301 gttgaccaca aggtcgcggt agctggcttg gctctgattg tcaataagga ggttgatgtt
    21361 gataacctaa cgacagagca acttcgtcaa atcttcatag gtgaggtaac caattggaaa
    21421 gaggttggtg gtaaggactt acccatctct gttatcaatc gggcagccgg ctctggttct
    21481 cgtgctacct ttgatactgt cattatggaa ggtcagtctg ccatgcaaag tcaggagcag
    21541 gattcaaatg gagcggtaaa atcaatcgta tcaaaaagtc caggagctat ctcttattta
    21601 tctcttacct atatagatga ttcggtcaaa agcatgaagt tgaatggcta tgacttaagt
    21661 ccagaaaata taagtagcaa taattggccc ttgtggtctt atgagcatat gtatacattg
    21721 gggcagccca atgagttggc tgcagaattt ctcaattttg ttctctcgga tgagacccaa
    21781 gaagggattg tcaaaggatt gaagtatatt ccgattaagg aaatgaaggt tgaaaaagat
    21841 gctgccggaa ctgtgacagt gttggaaggg agacaataat gaatcaagaa gaattagcta
    21901 agaaaatgtt gcttccatca aagaattctc gtctggagaa attaggaaaa ggtttgacct
    21961 ttgcctgtct ttctttgata gtcatccttg tggccatgat tttggttttc gtagcgcaaa
    22021 aaggcttgtc gaccttcttt gtcaatggtg tgaatatctt tgactttctt ttgggaggaa
    22081 cttggaatcc ttctagtaaa gaatttggtg cccttcctat gattttgggt tcctttatcg
    22141 ttaccattct ctcagccctt atcgcaacac cctttgctat tggtgcagca gtttttatga
    22201 ccgaagtatc accaaaaggg gcgaagattt tgcaaccagc tattgaactc ctggttggga
    22261 ttccttcagt agtgtacgga tttattggct tgcaagtcgt cgttcccttt gttcgcagtg
    22321 tctttggtgg gactggtttt gggattttgt cagggatttc cgtcctcttt gtcatgattt
    22381 tgccgaccgt aacctttatg acaacggata gcttgcgtgc ggttcctcgt tattatcgtg
    22441 aagccagttt cgctatggga gccactcgct ggcagactat ctggcgtgtg atcttgaagg
    22501 cggcccgttc tggtattttc actgcagtgg tctttgggat ggcgcgtgcc tttggtgagg
    22561 ctctagctat ccagatggtt gtcggaaact cagctgttat cccaacttcc ttgaccacac
    22621 cagctgcaac tttaacttct atattaacta tgggaattgg gaacactgtc atgggaactg
    22681 taaataataa tgttctctgg tcactggcct tggtactgct cttgatgagt ttagttttta
    22741 acagtgtgat taaattgatt acgaaagaaa gaggaaagaa aaattatgcg cgctaagaaa
    22801 ttagataaac ttgcaacagc tgtcctctat acgattgcta gcatcattgt gacaatcttg
    22861 gcttccttga ttctctatat cttggttcgg ggcttgcccc atatctcttg gtctttcttg
    22921 actggaaggt cttctgcttt tcaagcaggt ggtgggattg gcattcagct ttacaattcc
    22981 tttttcctat tggtcattac cttgattatt tctgtacctc tttctatggg agctgggatt
    23041 tacttggctg aatatgctaa aaaaggtcct gttaccaact ttgtgcggac ttgtattgaa
    23101 atcttgtcct ctttaccatc agtggtggtg ggtctctttg gttacttgat ctttgtagtc
    23161 cagtttgagt atggattttc aatcatttca ggtgccttgg ccttgacagt ctttaacttg
    23221 cctcagatga cgcgtaatgt agaagatagt ttgaaacacg ttcaccatac ccaacgtgag
    23281 gctggtctgg ctcttgggat ttctcgctgg gagacagtgg ttcatgttgt tattccagaa
    23341 gcgcttccag gtattgtaac gggtgtcgtc ttggcatctg gtcgtatctt tggcgaagct
    23401 gcagctctga tctatacagc agggcaatcg gcgccagctc ttgactggtc taactggaat
    23461 atcctcagtg tgactagccc catctctatc ttccgtcaag cagaaacctt ggctgtccat
    23521 atctggaaag tcaatagtga aggcactatt ccagatggaa caattgtatc agcaggttct
    23581 gccgctgtgc tcctgatctt tatcctgatt tttaactttg gagctcgtaa gttcggaagc
    23641 tatctacaca agaaattaac cgctgcctaa aggagaagcc atgtcaacat ataactggga
    23701 tgagaagcat atccttacct ttcctgaaga aaaagtagcc ctttctacta aggatgtcca
    23761 tgtttactat ggtaaaaatg aatccattaa ggggattgat atgcaatttg aaagaaataa
    23821 aattacagct ttgattggcc cgtcgggatc ggggaaatct acctacttac gcagtctcaa
    23881 tcgcatgaat gatactattg atattgctaa agtaactggg cagattctct atcgtggaat
    23941 tgatgtcaac cgtccagaaa tcaacgttta tgaaatgcgt aaacacattg gaatggtttt
    24001 tcaacgcccc aatccatttg ctaagtcaat ttaccgtaat attacctttg cgcatgaacg
    24061 tgctggagtt aaggataagc aagtcctaga tgaaatcgta gaaacctccc ttcgtcaggc
    24121 tgccctctgg gatcaggtta aagacgatct ccacaagtca gccttgacct tatcaggtgg
    24181 tcagcaacaa cgtctctgta tcgctcgtgc catctctgtt aagccagata tcctcttaat
    24241 ggatgagcca gcctcagcct tggatccgat tgcgaccatg caactagaag agaccatgtt
    24301 tgagctcaag aaaaacttta ccatcatcat tgtaacgcat aatatgcagc aggctgctcg
    24361 tgcaagtgac tatacaggct tcttttactt gggtgatttg attgagtatg acaagactgc
    24421 aactattttc caaaatgcca agctacagtc caccaatgac tatgtatctg gtcactttgg
    24481 ttagaaagga aaccgtatga cagatgcgat tttacaggta tcagacctgt ccgtttatta
    24541 taataaaaag aaggctttga atagtgtttc cctatctttc caacctaagg aaattacagc
    24601 cttgattggt ccatctggat cagggaagtc aaccctcctc aagtctctca accgcatggg
    24661 agatctcaat ccagaggtga ccacaactgg atccgtggtg tacaatggtc acaacatcta
    24721 cagtccgcgt acagatacgg ttgaattacg taaggaaatc ggaatggttt tccaacaacc
    24781 taatcctttc cctatgacta tctatgagaa tgttgtctac gggcttcgta tcaatggaat
    24841 taaggataag caggttctgg atgaagccgt agaaaaagcc ttgcaaggtg cctctatctg
    24901 ggatgaggtc aaggatcgtc tatatgattc agctattgga ttgtcaggtg gtcaacagca
    24961 gcgtgtctgc gtggcccgtg tcttggcaac tagtcctaaa atcatcctct tggatgagcc
    25021 aacttcggct ttggatccga tttcagctgg taaaattgag gaaactttgt atggtctaaa
    25081 agacaagtac accatgcttc tggtaacccg ttccatgcag caagcttcac gtatctctga
    25141 taagacagga tttttcctag atggagattt gattgaattt aatgatacca agcagatgtt
    25201 ccttaatccc caacacaagg aaacggaaga ctatattaca ggaaaatttg gataaggaga
    25261 tgaaagatgt tacgatctca atttgaagaa gatttagaga aattacataa ccagttctac
    25321 gctatgggac aagaagtgct ctcacaaatc aatcgtacgg tacgtgcttt tgtcacgcat
    25381 gaccgtgacc tggcaaaaga ggtcatcgaa gatgatgcag aagtaaatga atacgaagtg
    25441 aaactggaaa agaaatcatt tgaaatgatc gcactccaac aaccagtctc tcaagatttg
    25501 cgtacagtct tgactgtcct taaggctgta tcagatgtgg agcgtatggg ggatcacgct
    25561 gtagccattg ctcaggcaac catccgtatg aagggggaag agcgcattcc agctgtagag
    25621 gaagaaatta aaaaaatggg acgtgaagtt aaaagcgttg ttgaagcagc acttgatctt
    25681 tatcttaatg gttctgttga cgacgcatac cgggtggcct ccatggatga gcaaattaac
    25741 cactattttg aaactatccg tgaccttgcg actgaagaga ttaagaaaaa tccagaagcc
    25801 attgttacgg gtcgtgatta tttccaagtt atttcctact tggagcgtat cggtgactat
    25861 gctaaaaata tctgtgaatg ggttgtctac tttgaaacag gtaagattgt cgaactataa
    25921 aatagtttaa ttctgtataa aaaggagctt gaaatcaact gatggctcct ttttttgaac
    25981 gaaaaaatat ttttgagata aaaatttaaa aaacacttga caagtaactt ggatatcgtt
    26041 ataattatac aaaattaaca gagaggttgt ttatttatga aatcaaaaaa atggatattt
    26101 gttttatgta attttcttgc aagtttcttc ttagtagctt gccagtcagg ttctaatggt
    26161 tctcagtcag ctgttgaggc cattaagcaa aaggggaaat tagttgtggc aaccagtcct
    26221 gactatgcac cctttgaatt tcaatcattg gttgatggaa agaaccaggt agtcggtgca
    26281 gacatcgaca tggctcaggc tatcgctgat gaacttgggg ttaagttgga aatctcaagc
    26341 atgagttttg acaatgtttt gaccagtctt caaactggta aggctgacct agcagttgca
    26401 ggaattagtg ctactgacga gagaaaagaa gtctttgatt tttcaatccc atactatgaa
    26461 aacaagatta gtttcttggt tcgtaaggct gatgtggaaa aatacaagga tttaactagc
    26521 ctagaaagtg ctaatattgc agcccaaaaa gggactgttc cagaatcaat ggtcaaggaa
    26581 caattgccaa aagctcaatt aacttcccta actaatatgg gtgaagcagt caatgaattg
    26641 caggctggaa aagtagatgc tgttcatatg gatgagcctg ttgcacttag ttatgctgct
    26701 aaaaacgctg gcttagctgt cgcaactgtc agcttgaaga tgaaggacgg cgacgccaat
    26761 gccgttgctc ttagaaaaaa tagtgatgat ttgaaagaag tggtagacaa ggtcatccaa
    26821 aaacttaagg atgaaggaat ttaccaaagc tatcttgaaa aagcagcaag tctaactgaa
    26881 gttgaagaat aagaaaaagc agagttggat gtcattccaa ttctgctttt aaatagttta
    26941 aaacattttc cagattgtct agggacacct tggcaaattg ataaggacag gtacttaaat
    27001 aggcctcttc aaagaagtcc aagcccggtt gactgtgttt gagactggcg attttaggat
    27061 actgtctcag gtctagcaat aaaccatcgt gtaggggata atgaggaagg ggacaaggtg
    27121 ttttagttat cctttcctcg atttgttttt ggtaagattc atgattggtc aagcgagcca
    27181 aacgattttt ttcaaacaat tgactgtcaa tatagagtga cagggccttt tgcataatga
    27241 ggttgctgtc gtagtctagg atatttttgt aggccataaa tgcttctttg atagcatttg
    27301 gaagaatgag tgctgtaata cgaagggcag gaaaaaggct ggtcgagaag gacttgatat
    27361 aaatgacacg ctcctctgta tcaagatagt ggaaggtttg gcccttcttg gagtccaaat
    27421 cacccagata atcgtcttct acgatataga catcatactt ggcagctaag ttaagaatgg
    27481 atcgtttgtc ttgctcagaa taggaatgtc ccagaggata gtgaaatcgg ggaatggtgt
    27541 agaaaaactt aatttttcct gttttgaagt ggccttccag ctcctccaag tcaatcccat
    27601 caatgcctcg ttcaatcgtt tgatagtcca gcccctgtgc aatcaagagg cgattcatcc
    27661 gatggtaggt tggctgttcc accaagattt ccttggcttg tctaggaaag gatatttgag
    27721 agaggataaa taaggcttgt tgggttccag aagtcagtac tagttggtta gccttgcagt
    27781 agagagcttg ctcaaagagg agtttgtgaa tggactgtct taggtcttct aatccttctt
    27841 gattgtcata gtagttgaag aggtagtttt ctcgtccaat caaggtttca ttgacacaga
    27901 gtcggaaatc gtcataggca ctggcatgtt cgtcggtaac ctcgatttct aggtcttgat
    27961 gttgcccttg ttctaataca tagtagccac tctgaggctt ggcatagagg tattgttcgt
    28021 gccgtaattc cagcagggct cgttgaatgg tgtccttgct gcagtgaaag tcaaggctca
    28081 gttgacggat agaaggcagg cgactacccg tcggaaatcg tccagactcg ataccatttt
    28141 tcagatagga aacgacctct ttgtacttgc tttgtttctt cattccagac ctcccttgat
    28201 tttgttacat tgtacccttt tttttccttc ttggcaatgt ctagagcgtt tctctcgttc
    28261 acatctaggg gcaaatgtgg tatactccca tttttaatac ttttttgaaa tcaaagagca
    28321 atcaaatgca gatgtgacag tttttcgtac ccttttttgt ctttaaaaca acatgtgtat
    28381 ttttatctgg ggttgatttg ttttaaaaag tttttatgct aatttcccct tattacttgt
    28441 acatattact tgtacatatt tttaggaaaa tgcttgaaat cgttttcttg ttgtggtata
    28501 atagaactaa aaaataaaag catacgaata acttgttaga aaggagtttt aaatcagaaa
    28561 atcaagttat tttatcagta atatatattt gtgacttgaa aggagtgttt tttatgaaat
    28621 ttaaaaaaat tgtaacttca gctcttgttg tattatcact attttctatt atgtctcctt
    28681 ctatagcttc tgttagagta tttgctgatg atgtaactac tacggaagtt tctgaaatta
    28741 gtacccaaga acaaaaacaa atcgatgatg tagccaatgt tcttgaacaa atgtttagaa
    28801 atggagttaa tgaaaaaaat tttactgaat atgtttataa aaacttttct caaaaagata
    28861 ttgctcttgc ggaaaatgaa ttagaaacta atattaataa tccctatgac agagtccctt
    28921 gggatgagat gggaggatgt atagcaggaa aaatacgaga tgaatttttt gctatgatca
    28981 atgtttcgct aattgttaag tatgcgcaga aaaaggcttg gtcagaatta gctaaagtgg
    29041 tactaagatt tgttaaagct aatggactta aaacaaatat ttatatcatt gctggtcagt
    29101 tggctatttg ggcagttcag tgtggtctgg aataatgcgt gataaaaact ttttaataaa
    29161 agatgggatt attagctttt tgattggatt tgtatatgca tatttagtgt tacaaaaatt
    29221 tatcctttgg aaaatccttt tgtttgcatt tatttttgca attattaata caatatttat
    29281 gtttactagg aacaaaaaat aaataatgat ttagttaaat aaaaagcgac ccctagggaa
    29341 gtgtgttcct taggggttgt tgtgctagtg caagttttgt ttctgtcaaa atctcaggct
    29401 acccgtcgga aatcgtccag actcgatacc atttttcaga taggaaacga cctctttgta
    29461 cttgctttgt ttcttcattc cagacctccc ttgattttgt tacattgtac cctgtttttc
    29521 cttcttggca atgtctagag cgtttctctc gttcacatct aggggcaaat gtggtatact
    29581 taggagtaag atttatagaa taacagacaa gaaagtgaat ggataagaac gtgcaggaac
    29641 cagtgaaatt atttcaatac aatactcttg gagccttgat ggctggcctt tatggtggta
    29701 ctatgacagt aggagaattg ctagaacatg gtgaccttgg tttgggaacc ttggattcca
    29761 ttgatgggga attgattgtc ttggatggca aggcttatca ggccaaaggg tcaggagacc
    29821 agccagaaat tgtggaagtg tcaccagatg cccttattcc ttacgctgca gtggtaccgc
    29881 atcaggcaga ggtcattttc cgccagcgct ttgagatgac agacaaggaa ttgaaagaac
    29941 gaattgagtc ttattatgat ggggaaaatc ttttccgctc tatcaagatt cggggggaat
    30001 tttcacatat gcatgtgcgt atgattccta agtcaacacc cgataccaag tttgctgatg
    30061 tcgcaaccca tcaaccggaa tatagtcgtg acaatgttgc ggggactatt gttggtttct
    30121 ggacgcctga gattttccat ggggtcagtg tggcaggcta ccatctgcac ttcatatcag
    30181 atgatttgac cttcggtgga catgtcatgg attttgtcat caaggaaggc attatcgagg
    30241 tgggagcagt tgaccagttg gaccaacgtt tcccagtcca agaccgtcaa tacttgtttg
    30301 ctaagttcaa tgttgacgag atgaaaaaag atattgaaaa ggcagaatag gagaagaaaa
    30361 tgaccattca tatcattatt accatgttgc tgttgctagc ttttctgata ggaagcattt
    30421 ggtttgccaa aaagaaatac cagattaatc tagctgtctt gggcttgggt gcagtcgctt
    30481 tctttgtctc ttcgcagatt ttggaaaaac tggtgcatat cttgattttg catcctcaaa
    30541 aagacggtag tattgccctc ttgcaagatc atccgcttat ctatattatc tatggcctag
    30601 ccatggcagc attttttgag gaaacggctc gtcttgtttt cttcaaatgg ttggagaaaa
    30661 agagaagttt ggaaaaggca gatgccttgg cctatggcct agggcatggt ggcttggagt
    30721 tgatttttct aggccttact agtttactca atctttacat cgttctttcg gcagttcaaa
    30781 ctcagaatcc acaggttatg caattactgt ctgaaaatat gttaaaaacc attcagtcac
    30841 tttcggtctg gcagatttat ttgcttggtt ttgagcgaat cttggcgctt ggtttccaat
    30901 tacttttgac agtttgggtt taccaagctg ttcgccagaa gaaatggatt tatctcctag
    30961 cagcctatgg cttgcatgcc ttctttgatc tggcaccatc tcttttccaa gtaggctggt
    31021 tgacaaatcc agtcttggtt gaagtgattc tagcactgga gctcgttctg gtcgcctatg
    31081 gaaccaagga aatcttttgt aaaaaatcat aaaaaggggg aaacctcttt ttcttatgca
    31141 aaatccaaac aaggtatttt tatggtcgtc aaatgtctct aaaaatggta taatggaatg
    31201 aattttgtaa aaggaagagt gacatgtctg taagagaaaa aatgcttgaa atcttagaag
    31261 gaattgatat ccgttttcag gaacccttgc atagctatag ttatacaaaa gtaggtggag
    31321 aggctgatta tttggtcttt ccacgaaatc gttttgagtt ggctcgcctt gtgaaatttg
    31381 ccaaccaaga aaatatccct tggatggttc ttggcaatgc cagcaatatc atcgttcgtg
    31441 atggtgggat tcgtggattt gtcatcttgt gtgacaagct caataacgtt tctgttgatg
    31501 gctataccat tgaagcagaa gctggggcta acttgattga aacaactcgc attgccctcc
    31561 gtcatagttt aactggcttt gagtttgctt gtggtattcc aggaagcgtt ggcggtgctg
    31621 tctttatgaa tgcgggtgcc tatggtggcg agattgctca catcttgcag tcttgtaagg
    31681 tcttgaccaa ggatggagaa atcgaaaccc tgtctgctaa agacttggct tttggttacc
    31741 gccattcagc tattcaggag tctggtgcag ttgtcttgtc agttaaattt gccctagctc
    31801 caggaaccca tcaggttatc aagcaggaaa tggaccgctt gacgcaccta cgtgaactca
    31861 agcaaccttt ggaataccca tcttgtggct cggtctttaa gcgtccagtc gggcattttg
    31921 caggtcagtt aatttcagaa gctggcttga aaggctatcg tatcggtggc gtagaagtgt
    31981 cagaaaagca tgcaggattt atgatcaatg tcgcagatgg aacggccaaa gactacgagg
    32041 acttgatcca atcggttatc gaaaaagtca aggaacactc aggtattacg cttgaaagag
    32101 aagtccggat cttgggtgaa agcaagtagg gagctgtatt tgaaaagctg ctgctatgtc
    32161 atggaaaggg gggtgaaagc ctatcggtag cgaagatgta tgcaggtggt tttactccct
    32221 gcaagaggta gtggcgacct gacagagccc cgatctgtta atctatgaaa aagaaggaat
    32281 ttataccaat tgaaaaaacc aattatcgaa ttcaaaaacg tctctaaagt ttttgaagac
    32341 agcaacacca aggttctcaa agacatcaac tttgagttgg aagaagggaa attctacacc
    32401 cttctaggtg catctggttc ggggaaatca actatcctaa acattattgc aggtttactg
    32461 gatgcgacga caggagatat catgctagac ggtgttcgta tcaatgatat tccaaccaac
    32521 aagcgtgatg tccatacggt cttccaatcc tatgccttgt ttccgcatat gaatgtgttt
    32581 gaaaatgttg cctttccact tcgcttgcgc aagattgaca agaaagaaat cgagcaacgc
    32641 gtagcggaag ttctcaagat ggttcagttg gaaggttatg aaaaacgttc catccgcaaa
    32701 ctttctggag gacaacgtca gcgtgtggct atcgcccgtg ctatcatcaa ccaaccccgt
    32761 gtggttttgt tggatgagcc cttgtcagcg ctggacttga aattaagaac agacatgcag
    32821 tacgaactgc gtgaactgca acaacgattg ggcattacct ttgtctttgt cactcacgat
    32881 caggaagaag ctcttgccat gagtgactgg atttttgtta tgaatgatgg cgagattgtc
    32941 cagtctggaa ctcctgtgga tatttatgat gaaccaatca accactttgt tgccaccttt
    33001 atcggggagt caaatatctt gccaggtacc atgattgagg actacttggt tgagtttaac
    33061 ggcaaacgct tcgaagcggt cgatgggggg atgaagccaa atgaacctgt tgaggttgtt
    33121 attcgtccag aggacttgcg cattaccctt cctgaagaag gcaagctcca agttaaggtc
    33181 gatacccagc ttttccgtgg agttcattat gaaattatcg cctatgacga acttggaaat
    33241 gaatggatga tccactcaac ccgtaaggcc atcgtgggtg aggaaatcgg tctggacttt
    33301 gaaccagaag acatccacat catgcgtctc aatgaaaccg aagaagagtt cgatgctcgt
    33361 attgaggagt acgtagaaat cgaagagcaa gaagcaggtt tgatcaatgc aatcgaggag
    33421 aaaagagatg aagaaaacaa gctctaaact ctttgtagtg ccctacatgc tttggattgc
    33481 gctctttgta ttggcaccct tggtcttgat tttcggtcaa tcctttttca acatcgaagg
    33541 ccagttcagt ttagaaaatt acaaatctta ctttgcgtca caaaacttga cctatcttaa
    33601 aatgagtttc aactcagtgc tttatgcagg cattgtgacc tttgtgacac tgcttatcag
    33661 ctatccgacg gccctctttt tgacccgtct caaacaccgt caactctgga ttatgctgat
    33721 tattctgcca acttggatca accttcttct taaggcttat gcctttatcg ggatttttgg
    33781 tcagaatggc tctattaacc aattcttgga atttatcgga attggttcac agcagttgct
    33841 atttactgat ttctcattta tctttgtcgc aagctacatc gagctcccct ttatgatttt
    33901 gccgattttc aatgtcttgg acgatatgga taataatctc atcaatgcta gttacgacct
    33961 tggtgcaact aagtgggaga ctttccgtca tgtcatcttc cctctatcta tgaacggtgt
    34021 gcgaagtggg gttcagtcgg tctttatccc aagtttgagt ctcttcatgc tgacccgttt
    34081 gattggtggg aaccgcgtta tcaccttggg gacggctatc gagcagaatt ttctaaccaa
    34141 tgacaactat ggtatgggtt cagccattgg tgtgattctc atcctgacca tgttcatcac
    34201 catgtgggtg accaaggaaa ggagagaacg atgaaaaaat ttgccaacct ttatctggga
    34261 ctggtctttc ttgtcctcta cctgccgatt ttttacttga ttggctacgc ctttaatgca
    34321 ggaaacgaca tgaacagctt tacaggcttt agcttgagcc attttaaaac catgtttggt
    34381 gatggtcgcc ttatgttaat tgtgactcag acctttttct tggccttcct atcagccttg
    34441 atagcgacca ttatcgggac ttttggtgcc atttacatct accagtctcg taagaaatac
    34501 caagaagcct ttctatcact caataatatc ctcatggttg cgcctgacgt tatgattggt
    34561 gctagcttct tgatactctt tacccaactc aagttttcac ttggcttttt gaccgttcta
    34621 tctagtcacg tggccttctc cattcctatc gtggtcttga tggtcttgcc tcgactcaag
    34681 gaaatgaatg gcgacatgat tcatgcggcc tatgacttgg gagctagtca atttcagatg
    34741 tttaaggaaa tcatgcttcc ttacctgact ccgtctatca ttgcaggtta cttcatggcc
    34801 ttcacctatt cgctggatga ttttgccgtg accttctttg taacggggaa tggcttttca
    34861 accctgtcag tcgagattta ctctcgtgca cggaaaggga tttccttaga aatcaatgcc
    34921 ctgtctgctc tagtctttct ctttagtatt atcctagttg taggttatta ctttatctct
    34981 cgtgagaagg aggagcaagc atgaaaaaaa tctattcatt tttagcagga attgcagcga
    35041 ttatccttgt cttgtgggga attgcgactc atttagatag taaaatcaat agtcgagata
    35101 gtcaaaaatt ggttatctat aactggggag actatatcga tccagaactc ttggagcaat
    35161 ttacagaaga aacaggcatc caagttcagt acgagacttt tgactccaac gaagccatgt
    35221 acactaagat aaagcagggt ggaacgacct acgatattgc cattccaagt gaatacatga
    35281 ttaacaagat gaaggacgaa gacctcttgg ttccgcttga ttattcaaaa attgaaggaa
    35341 tcgaaaatat cggaccagag tttctcaacc agtcctttga cctaggtaat aaattctcca
    35401 tcccttactt ctggggaacc ttaggaattg tctacaacga aaccatggta gatgaagcgc
    35461 ctgagcattg ggatgacctt tggaagtcgg agtataagaa ttctatcatg ctctttgatg
    35521 gggcgcgtga ggtgctggga ctaggactca attccctcgg ctacagcctc aactccaagg
    35581 atccgcagca gttggaagag acagtggata aactctacaa actgactcca aatatcaagg
    35641 ctatcgttgc ggacgagatg aagggctaca tgattcagaa caatgctgct atcggtgtga
    35701 ccttctctgg tgaagccagc caaatgctag aaaaaaacga aaatctacgc tatgtggtac
    35761 cgactgaagc cagcaacctt tggtttgaca atatggtcat tcccaaaaca gtcaaaaacc
    35821 aagacgcagc ctatgccttt atcaacttta tgttgaaacc cgaaaatgct ctaaaaaatg
    35881 cggagtatgt tggctattca acaccaaacc taccagctaa ggaactgtta ccagaggaaa
    35941 caaaagaaga taaggccttc tatcctgatg ctgaaaccat gaaacaccta gaaatttatg
    36001 agaaatttga ccataaatgg acaagaaaat atagcgacct cttcctacag tttaaaatgt
    36061 atcggaagta ggaggctaaa cggatgaaaa cgaatcagtc aagctggttc gtttttaagg
    36121 aatagaagtc agtttttaga gccctatctt tttctattct agttgtttat ttgcttattt
    36181 gcttgcctag tagtatacta agcctatact cttcaaacca tgtcagcgtt gccttgccgt
    36241 actcaagtac agcttgaggc tagcttccta gtttgctttt tgatttttat tgagtattaa
    36301 ccttacagaa agcggcaaca aataatgaaa ctgtctactt ttttcaaaaa aagtttctgg
    36361 ccaaccttta tgattgttaa ccaaactgct atactattcc atttacaagg atgacttgga
    36421 tcgacaatac ctaacaactg agtccatata ttgggtgata ggcaccttta tatttggaaa
    36481 tattctagta gctgtgttta gtaatatgaa aatttgggat aaaaagaaaa acggcagtaa
    36541 gaaaaaatat atcctgaaaa agtaatcaga gatgtaggga attggtctaa ttcaacggag
    36601 atcatttcca gttgacgcaa gcttaaaaaa acgttataat aagaaagttg agaattgatt
    36661 ttcacttgtc ttagtccaga gaaatggcgg tgctgcgagc catctaagct aggaagtcat
    36721 gctactcaat catacaattg cataagaaca agagaatgac aagttcattg aatgaaggtg
    36781 gtaccgcggt ttttcgccct tcgtgatatg agcttgtctt ttgatttttg gaggtgtgga
    36841 tgaaaacatt tcttgtcaaa caaaagtttc gtcttggagg tgaacgcttc gctatcaagg
    36901 atgacagggg agaaattgcc tatcaggtgg agggatcatt ttttaagatt cccaaaactt
    36961 ttaccatcta tgatgcggct ggtgaacaag tcagtcagat cagtaaagaa atcttgacct
    37021 tgcttcctcg ttttgagatt cagcttcgag atggctcgag ttttgtcatt cgtaagaagt
    37081 tgaccttctg gcgagataag tatgagtttg ataatctagg tcttcgtatc gagggcaata
    37141 tctgggattt gaatttcaaa ttgctggatg atcgcgatca gttgattgcg gaaattaaga
    37201 aggaactctt ccatctgacc tctacctata ccgtaacggt tcttgaagac gcttatgcag
    37261 atctagtcat ttccctctgt gtcgcgattg actatgtgga aatgctggaa agccaatcac
    37321 attaaacaag taaataagga gacaatatga aacaactatc tagtgcacaa gtacgccaaa
    37381 tgtggcttga tttctgggcg accaaaggtc actcagtaga accatcagtc agtttggttc
    37441 ctgtaaatga cccaactctt ttgtggatca actctggggt agcaaccctc aagaaatact
    37501 ttgacgggac cattatccct gagaatccac gtattaccaa tgcccaaaag gctatccgta
    37561 ccaacgacat cgaaaacgta gggaagactg cgcgtcacca taccatgttt gaaatgttgg
    37621 ggaacttctc tatcggggat tacttccgtg acgaagctat cacttgggct tatgagcttt
    37681 tgacaagccc tgaatggttt gatttccctg ctgaaaaact ttacatgacc tactatccag
    37741 acgataaaga ttcttacaac cgctggattg aagtggaagt ggatccaagt cacttgattc
    37801 caattgagga caacttctgg gaaatcggtg cgggaccttc tggaccagat acagaaatct
    37861 tctttgaccg tggggaagcc tttgacccag aaaatatcgg tcttcgcctg cttgcagaag
    37921 atattgaaaa cgaccgttat attgaaatct ggaacatcgt tttgtcacaa tttaacgcag
    37981 accctgctgt tcctcgtagc gaatacaagg aattgccaca taagaacatt gatacgggcg
    38041 ctggtttgga gcgtttggtg gccgttatcc aaggggctaa gaccaacttt gaaacggacc
    38101 tcttcatgcc gattatccgt gaagtcgaga aattgtctgg taaggtttat gaccaagatg
    38161 gcgacaacat gagcttcaag gtcatcgctg accacattcg ttctctttca tttgctatcg
    38221 gtgatggtgc ccttccagga aatgaaggtc gtggttatgt ccttcgtcgt cttcttcgtc
    38281 gtgcttctat gcatggtcaa aaattgggta tcaacgagct tttcctttac aaactcgttc
    38341 caactgttgg aaaaatcatg gaaagctact acccagaagt gcttgagaaa cgtgacttta
    38401 ttgagaaaat cgttaagagc gaagaagaat catttgcccg tacccttcac tcaggtcaac
    38461 actttgccca aggcattgta gcagacttga aagaaaaagg tcaatctgtc attgctggtc
    38521 aagatgtctt taaactctat gatacatacg gattcccagt tgaattgact gaagaaattg
    38581 ctgaagaggc tggtatgact gtagaccgtg aaggttttga agcagccatg aaagaacaac
    38641 aagaacgcgc gcgtgcgtca gctgtcaagg gtggctcaat gggtatgcaa aatgaaactc
    38701 ttcaaaatat cactgtagaa agtgtcttca actacaatgc tagccaattg tcttctaaat
    38761 tggtagctat cgtggcggac aatgcagaag tagaagctgt ttcagaagga actgcctctc
    38821 ttatctttgc ggaaacgtca ttttatgctg aaatgggtgg acaggtagct gaccacggac
    38881 aaatcttgga tgagtcaggt aaggttgtgg ctactgtgac caatgttcag aaagccccaa
    38941 atggtcaagc ccttcataca gttgaagtcc ttgcaccgct tgccttgaac caagaatata
    39001 ccttggcaat tgatagcaat cgccgtcacc gtgtcatgaa aaaccacact gcgactcatt
    39061 tgcttcacgc tgcccttcac aatatccttg gaaaccacgc aacacaggca ggatctctta
    39121 acgaagttga attccttcgc tttgacttta cccacttcca agctgtaact gctgaagaat
    39181 tgcgtgcgat tgaacagcaa gtcaacgaaa aaatctggga agctcttgaa gttaagacag
    39241 ttgaaacgga tattgacact gctaaagaaa tgggagctat ggccctcttt ggtgagaaat
    39301 acggcaagga agttcgtgtc gtgactatcg gtgactactc tattgaactt tgtggtggta
    39361 ctcatgttgg caacacttct gagattggtc tcttcaaaat tgtcaaagaa gaaggaatcg
    39421 gttcaggaac tcgccgtatc ttggcagtga ctggtaagga agcctttgaa gcctatcgtg
    39481 aacaagagga tgctcttaaa gctgtcgcag caaccttgaa agcacctcaa gtcaaggaag
    39541 tacctcacaa ggtagaagga cttcaagaac aacttcgtca acttcaaaaa gaaaatgctg
    39601 agttgaaaga aaaagccgca gctgcagccg caggcgatat cttcaaagat gttaaggaag
    39661 tcaacggtca tcgttacatt gctagtcaag tgtctgtatc cgatgccggt gcccttcgta
    39721 cttttgcaga taactggaaa caaaaagact actctgatct tcttgtccta gttgccgcta
    39781 tcggtgacaa agtcaatgtc cttgtagcaa gcaagacaaa agaccttcat gcaggaaacc
    39841 ttgtcaaaga attagcacca atcatcgatg gacgtggtgg tggtaaacca gacatggcca
    39901 tggcaggagg aagcaaccaa gctaagatcc aagaattgtt ggatgcagta gcaggtaaat
    39961 tgtaatcaag taaagatcta tccatttggg taggtctttt tgtgaataca aaaaagccaa
    40021 atccgcttgg acctagcttg gttattatga gctattagat ggtattgact gcccagacac
    40081 ttacggatct agctgagaca gggaattgtc cataaccttc ctcatcaatt gtaacttgac
    40141 cttggtggtt accaagtaaa tctacaaagg tttgattagt ccattcttga ccgacaaaca
    40201 ttgacttgct gttttcttgg tcatttgaga taaggactgc gattggggat tgattttcag
    40261 cacctgaacg tacccaaccg atacagttag catggtcaaa gtagtcattt tgttctccat
    40321 aggccaaatc ttttcggatg gctaggaggc ggtcaaggat ttctttgaaa tcttcttgag
    40381 catactggcc tgaaatccca tagtagtctc cgtaaaagac acatggaagg ccgtcttggc
    40441 gtaacaaaat gagggcatag gctgctggct tgaaccattc ttcaacggta gactcaaggg
    40501 cttgtcctcg ttgggtatcg tggttgtcga caaaagtcac agccttgtca ggcttgagtt
    40561 caaccaggct atctgtgaaa atgccacgaa ggtcatagtt tgcgccagct tgactggctt
    40621 caaagagatt ctggtggaga cgaacatcga caaggtcaaa gtgttcttcc gttttttcga
    40681 gatagtccag attggcttcc ttgtctgggt tccaaaattc accaaaaaca tagaaatcgt
    40741 caccatattt ttccttcata tcgcggatga agttgcgcat aaagaaagag tcaatatgct
    40801 taacggcatc caaacggaaa ccagctacac cagtcgtttc catgaaccaa tcagcccagt
    40861 catagatgtt ttggatgact tcaggatgtt taaagtctag gtcggcatac atgaggtagt
    40921 cgtagtttcc gttttcgtta tcgaccaatt cctcgttagc ccagcccttg ttgtcccctt
    40981 ggatcagata aatcccagat ttactgcgtt tggcatcgta gtctgtaccg gtgaagtggt
    41041 accaatgcca gtggaagcca ttataggtat cttggcgacc atcgaaggta aaactagtcc
    41101 agccattgat ggtgaagggt tctccaagtt caactgtacg gtctacagga tcaacttcga
    41161 tgacctgaaa ggcttccctg tgatcggcag cagccttgtg gttgagaact acatcggcca
    41221 taggttgaat tccctgtgct ttaagggctt gaatggcttg aagatagtct tctttgaaac
    41281 catacttggt gcggacagtc cctttttggt tgaactctcc taagtcaaat aagtcataga
    41341 ccccatagcc gacatctttt tcgttggttg ccttgaaggc tggtggcatc cagacgtgac
    41401 tgatccccag atgagctagg tgtggagcat tttcagccag acgcgtccag tgttgaccgt
    41461 cgtggggcag ataccattca aagtattgca tgagtgtttg attttgcatg atgtttcctc
    41521 ttacttgtca atcttgttct ttattctatc ataaagtatc tctcggcgca aacgtttgcg
    41581 taagatagaa ggaaactatt ttaactactt aaaataaagt attgattttt tgtatcaaag
    41641 tgctagtata gcgatgtata atacagataa agataggagt tgtcccatga ttgaatttca
    41701 aaatgttagt aagttgtatg gtaataaaga ggccttgagt aatctcaatt tgcagattga
    41761 aaatggagag attatgggct tgattggtca taatggggct ggaaaatcga ccactataaa
    41821 atccctagtc agtatcattt cacccagcag tggtcgtatt ttggtagacg gtcaggagtt
    41881 atcggaaaat cgcttggcta ttaaacgaaa gattggctac gtagcagact cgcctgactt
    41941 atttttacgc ttaacggcca atgaattttg ggaattgatc gcctcatcct atgatctgac
    42001 tagatctgac ttggaggcta gtctagctag gctattgaac gtttttgatt ttgctgaaaa
    42061 tcgctatcag gttattgaag ctctttctcg cggaatgcgt cagaaagtct ttgtcatcgg
    42121 agcactcttg tctgatcccg atatttgggt cttggatgaa cccttgactg gtttggatcc
    42181 ccaggctgcc tttgatttga aacagatgat gaaggaacat gcacaaaaag ggaagacagt
    42241 cttgttttaa actcatgtcc tagaggtggc agagcaagtc tgtgatcgga ttgccatttt
    42301 gaaaaagggg catttgattt attgtggtag tgtagaggac ttgagaaaag attacccagc
    42361 ccagtctttg gaaagtatct accttagtct tgctggtaga aaagaggagg ttgcggatgc
    42421 gtctcaaggt cattaaaaaa ttagttgata tcaatatcct ttattcatct caagaagcta
    42481 atctggctaa tctacgaaag aagcaggcta agaatcctgg gaaaaaagta aatgtttccg
    42541 ctagagtcct aagttcttac attttttcca gtctcttgat gctcatctgt tttagtaata
    42601 tagccattca ttttcctttt gaggaaatac ctatttattt tagcttgatg gttgctattt
    42661 tactagtgat tgccttttca acttctttaa cggcctttta caatgtcttt tatgagagta
    42721 aggatttggt atcgtatagg ccctatgcct ttaaagaatc agagattata attgctaaag
    42781 gactgtctgt cctcttgcca gctctaactg gaattgtacc aatcctagct tattttctag
    42841 ccctctatat taggctagct ccttctctat ggctgggttt gcctttgatg ctactgtcct
    42901 tgaccttatt atttgtctct gtggctctag tgatggtagt ggcagtgcat ttcttggctc
    42961 agactagggt cttcagaaag tatcagtcta ttttttcgaa tgtgatgatt gggataggag
    43021 ttctcatacc tttaatattt atcttctttc ttcagtcgac ttttggaagt attgttgaca
    43081 aagttagaga cattccattt ctcctttatc ctcttcatat cttttacaaa atagcagtgg
    43141 agcctttttc aatagaagcc atattgggtc tgctcgcttg gataggacta actctcttcc
    43201 tgctttatct gaccaaaaag aaggtccttc ctcgttttta tgacgtgatc ctgcttaata
    43261 gtgaggagaa ggtcaaaaaa gaacgtcgca gcaaggagag aatttcaacc actaaaaagg
    43321 gatttttccg catggtttta cgctaccacc tcaccctctt gggacagggg actggcgtgg
    43381 ttacagtgct ttttacaagt gctttccttc cttatctcat gatgatcggt ctgatttcca
    43441 aaatccgaga ttctcagata gttccagaca ttcatcctcc atactggtta cccttgtttt
    43501 ttatagctct ctttatagca gttgtcaata acaatatcac cagcctgcct tcaattgcct
    43561 tgtccttgga gagggaaaat gttgattttc tgaagagttt accctttgac tttgctcgtt
    43621 atgtgaaagt gaaattttgg attatctttg ctgttcagtc ctttttacca attctgactt
    43681 tacttggtct ttctctatat ctaggcttgc ccatcatttc gatgatttac cttattgtgg
    43741 catggatcct tgccagtgtc atcctttctt gtcaccatta ttttaaggat gtgaaaaatc
    43801 tgtcaaccaa ttggagtagc attacggacc tggtgaatcg ttcaaatggc atagtcgcca
    43861 tagttttatt gtttatttat agtgcaatcc tgatggccct tgtaattggg agtatattct
    43921 tggttcagtc tctctcccct atccttgcca tcagcttggg agtaggagct cttatcctcc
    43981 tgcttgctct tgctattttt ggctatcatt attacctgtc acgcatattg gcagaaatag
    44041 aaaaaagatg agctggttgg tcgcttgctt gataaattca attcgacaaa cgcttttata
    44101 gtttgttaga atgtagttgt aaatgattaa gattggtaat caaaaatata gggaggaatg
    44161 ttttgaataa gaaatataat atagttctat tattgttgtt tatagtttat ttattgggat
    44221 atttttcaat ttcaaaaacg ttaattccta taatgtgtgt gatccaagta tttttgatag
    44281 aacatatatt tagagttcga aatagaatga tgcaaatagg tgaaatttta attattgttg
    44341 cttctataat attatttatt gatagtatat tgagtttgta acaaaaggct tttctataaa
    44401 atcaaatttt ccagatatct actgaagctg atttatttct tcatctttct tctactacct
    44461 ttgtcactaa atttgacaga cacatattgc tgatacaacc tctctaccta taatctcacg
    44521 gttaacctga ggctagtttc atagtttgct atttgatttt cgttgataaa aagaaatgct
    44581 cctccaagac ggaagagcat tttttattgt gattcttaca catttatttc ctagttttgc
    44641 aggaagtcta gtataccatt ttttaaaaga agagtaaaaa agcaagggta gtttcttttt
    44701 tgagaattgt ctaaaagttt gatataatgg tagttatgaa tagaataaga gtcagcaaaa
    44761 gggttgaaaa gaagcttgct aaggggctag ttttactaga agccagtgat cttgagaatg
    44821 tcaatcttaa ggatcgggaa gtagaggtgc agggtcagga aggaaacttt cttgggactg
    44881 cctacctttc tcagcaaaac aagggcttgg gctggtttat cagcaaagac aaggtgacct
    44941 tcaatcaagc tttctttgaa acgttgttta gaaaagccaa agaaaagaga aacgcctact
    45001 atcaagatga tttgacaact gcctttcgtc tctttaatca agagggagat ggctttgggg
    45061 gtctgacagt ggacctttat ggcgactacg ccgtcttttc ttggtataac tcttatgttt
    45121 atcagattcg tcagactata tcagaagcct ttagacaggt tttccctgag gttttagggg
    45181 cttatgagaa aatccgcttt aagggttttg actatgaatc tgcccatgtt tatggtcaag
    45241 aagcacctga ctttttcact gttttagaaa atggtgtcct gtatcaagtc tttatgaatg
    45301 atggcttgat gacagggatt ttcctagacc agcatgaggt tcgcggtagt ttagttgacg
    45361 gcttggctat gggtaaatcc ttgctcaata tgttttccta cacagcggct ttttcagtag
    45421 ctgcggccat gggaggagct agccagacaa cttctgttga tctagccaaa cgttcacgag
    45481 aattgtctca agcgcatttt caggcaaatg ggctcagcac agacgagcat cgttttatag
    45541 tcatggatgt ctttgagtat ttcaaatatg ccaaacgcaa agacttgacc tacgatgtga
    45601 ttgtcctaga tccgcctagc tttgctcgga ataaaaaaca aactttctct gtggccaagg
    45661 attatcacaa gttgatttcc cagagtcttg agattttaaa tccgggaggg attatcattg
    45721 ccagtaccaa tgctgccaat gtttcccgtc agaaatttac agaacaaatt gataaaggtt
    45781 ttgcaggaag aagttaccag attttaaaca aatatggtct tccagcagat tttgcctata
    45841 ataaaaaaga tgaaagtagt aattacctca aggtgattag tatgaaggtt agtaaatgaa
    45901 attaatcgtt tcagtaatgc caagaagttt agaggaggct caggctctgg atgccacgag
    45961 gtacctggat gccgacatca ttgaatggcg tgccgactat ctgcctaaag aagcgatttt
    46021 gcaggtagct ccagccattt ttgaaaaatt cgcaggccgt gagttggttt tcacgctacg
    46081 aactcgctcc gaaggtggag aaatcgacct ttctccagaa gagtatatcc atctaatcaa
    46141 ggaagttgcg caactctatc aaccagacta tattgatttt gagtactata gctacaagga
    46201 tgtttttgag gaaatgctgg acttcccaaa tcttgttttg agttaccata atttccaaga
    46261 aacacctgag aacatgatgg aaatcttgtc agagttgacg atcctaaatc caaaacttgt
    46321 taaggttgcg gtgatggctc acacggagca ggatgtctta gacttgatga actatacacg
    46381 aggctttaaa accctcaatc ctgaacagga atatgtgacc atttctatgg gtaaggtggg
    46441 caaggtctct cgtatcactg cggatgtgac tggttcgagt tggtcttttg ccagtctgga
    46501 tgaggttagt gcccctgggc agatttctct agctagcatg aaaaaaatca gggagatttt
    46561 ggatgaagct tgatggctat acacgtttag ctgcagttgt tgccaatcct attaagcatt
    46621 ctatttctcc cttcatccac aatagagcct ttgaggcgac agctaccaac ggtgcttatg
    46681 tggcttggga gattgaagcg agtgacttgg cagaaacagt ggccaatatt cgtcgctacc
    46741 agatgtttgg catcaatctg tccatgccct ataaggagca ggtgattcct tatttggatg
    46801 agctaagcga tgaagcgcgc ttgattggtg cggttaatac ggttgtcaat gagaatggca
    46861 atttaattgg atataataca gatggcaagg gattttttaa gtgcttgcct tcttttacaa
    46921 tttcaggtaa aaagatgacc ctgctgggtg caggtggtgc ggctaaatca atcttggcac
    46981 aggctatttt ggatggcgtc agtcagattt cggtctttgt tcgttccgtt tctatggaaa
    47041 aaacaagacc ttacctagac aagttacagg agcagacagg ctttaaagtg gatttgtgtg
    47101 ctttagaata tgtttctgaa ctgcaagcaa ggattgccga gtcggatttg ctagttaatg
    47161 ccaccagtgt gggcatggat ggccaatcct ctcctgttcc tgaaaacata gtcttaccag
    47221 aaactctctt agttgcagat atcatatacc aaccctttga aacaccgttt ttgaaatggg
    47281 ctagaagtca gggcaatcca gcagtcaatg gtctgggaat gttgctctat caagctgcag
    47341 aagcttttca actgtggaca ggcaaggaaa tgccgacaga agagatttgg cagtctttaa
    47401 cagaaaaata ccaataaaga aagggagact ctcctatgaa aatcagaatc gatattcctc
    47461 atcatcctta tgatattcag attgaaaaag gttgtatggc ccaggctggt cagtggttgc
    47521 gagaactctg gcaaccgcaa aaggtagtca ttgtgacaga taaccatgta gcctctctct
    47581 atgcagagaa ggtcaagctc agcctagaag atgctggttt tcaggtagct gtttttgatt
    47641 tcttagaagg tgaagaaaga aagaatttaa ctactgttca gaaagtctat gaatttctag
    47701 tcaagcaagg tctgactcgt agcgatggaa tcgttgctct tggtggtggc gttgttgggg
    47761 acctggctgg ctttgtagcc tctacctata tgcggggtat tcactttgtt cagattccga
    47821 ctagtttgac agctcaggtt gattcttcta tcggtggaaa gacaggcgtt aacacaccat
    47881 ttgctaaaaa tatggtgggg acctttgccc aaccagatgg ggttctgatt gatccacttg
    47941 ttcttgaaac cctcggaaaa agagagttga ttgaagggat gggtgaagtt atcaagtatg
    48001 gcttgattga ggatccagaa ctgtgggctc tcttgacggg actgaatggt tctgttgaga
    48061 gtattttgga acatgcagag accttgattg aacattcttg tcaggtgaag cgcaagatgg
    48121 tggttgaaga tgagttggac aatggtattc gtctttacct caattttggc cacactattg
    48181 gccatgccat cgaagcgact gccggttatg gcaaggtcat gcatggagag gctgttgcca
    48241 tgggaatggt acagatttcc aaggttgctg aggaaaaagg cctcatgcca gctggcatta
    48301 cccaatctat cacagagatg tgtcagaaat tcggcttgcc tgttgactat gaaaattggg
    48361 aagttgacaa gctttatcag gctcttactc atgacaagaa agcgcgtggt aacaccttga
    48421 aattggtctt ggtgccagag cttggttcag cgatcattca cccagtttct ctggaagaga
    48481 tgaaagacta cttggtaaaa taaggagagt gtatgagata tttaactgca ggagaatcac
    48541 acggcccccg tctaacagct attattgagg gaattccagc tggacttcca ttgacagctg
    48601 aggatatcaa tgaggacctt agacgccgtc agggtggcta cggtcgtggt ggtcgtatga
    48661 agattgagag tgaccaggtt gtctttactt cgggcgttcg ccacgggaag acgacagggg
    48721 cgcctattac tatggatgtc atcaataagg accaccagaa atggctggac atcatgtctg
    48781 cggaggacat tgaagaccgc cttaaaagca agcggaaaat tactcatcct cgcccaggtc
    48841 atgccgattt ggttgggggg attaagtacc gttttgatga tttgcgaaat tctttggagc
    48901 gttcatcagc tcgtgaaacc accatgcggg tggcagttgg tgcagtagcc aaacgcctct
    48961 tagctgagct ggatatggag attgccaacc atgtcgtggt ctttggtggc aaggaaatcg
    49021 atgttcctga aaatctgaca gtcgctgaaa ttaagcaaag agctgcccag tctgaagttt
    49081 ctattgtcaa ccaagaacga gaacaggaaa tcaaggacta tattgaccaa atcaaacgtg
    49141 atggtgatac catcggtggg gttgtggaga cagtcgtcgg aggcgttcca gttggtcttg
    49201 gttcctatgt ccaatgggat agaaaattgg atgcaagatt ggctcaagct gttgtctcta
    49261 tcaatgcctt taaaggggtg gaatttggtc ttggctttga ggctggttat cgtaaaggca
    49321 gccaagttat ggatgaaatt ctctggtcta aagaagacgg ttatactcgc cgtaccaata
    49381 atctaggtgg ttttgaaggt ggtatgacta atgggcaacc catcgttgtt cgtggggtca
    49441 tgaaacccat tcctactctt tataaacctc ttatgagtgt ggatatcgaa acccacgaac
    49501 cttacaaggc aaccgtggag agaagtgatc cgactgctct tccagctgca ggaatggtca
    49561 tggaagcagt tgtagcaacg gttctggcgc aagaaatcct cgaaaaattc tcatcagata
    49621 atcttgagga actaaaagaa gcggtagcca aacaccgaga ctatacaaag aactattaag
    49681 gagttcctat ggcaaaaaca atctatatcg caggtcttgg gttgattgga gcctctatgg
    49741 cacttggtat caaacgcgat catccagatt atgaaatttt aggttataat cgtagtcaag
    49801 cttcgagaga tatcgccttg aaagaaggca tgattgaccg tgcaacggat gattttgcta
    49861 gttttgctcc tttggcagat gtcattatcc tcagcttgcc aatcaaacaa actattgctt
    49921 tcattaagga gttggccaat ttggatttgc gagaaggcgt tattatttca gatgctggtt
    49981 cgaccaagtc aaccattgtg gatgcggcgg agcagtattt ggctggcaag tctgttcgct
    50041 ttgtcggggc ccatcccatg gctggtagtc acaagacagg ggctgcttcg gcagatgtca
    50101 atctttttga aaatgcctat tatatcttta caccttcaag cctgacaagt caggacacgc
    50161 ttaaggaaat gaaggatctg ctttcaggtc ttcatgctcg ttttatcgag attgatgcca
    50221 aggagcatga tcgtgtcact tctcagatta gccattttcc tcatattttg gcttctagtc
    50281 tcatggagca gactgcggtc tatgctcaag agcatgagat ggcaaggcgc tttgcggcag
    50341 gtggttttcg agatatgacc cgaattgcgg aaagcgagcc aggaatgtgg acctccattc
    50401 tcttgtccaa tagcgagacc attctggata gaattgagga tttcaaggaa cgtttggaag
    50461 tgattggtca ggccattagt aagggagatg aagagcaaat ttggaacttt tttaaccaag
    50521 cgcgtgaaca acgccagacc atggaaatcc ataagcgtgg tggtgtggat agctcttacg
    50581 acctctatgt tgacgttcct gatgaagaag atgtcatcct gcggattttg gaactgctac
    50641 gtggaacttc cttggttaac atccacatca acgaggaaaa tcgtgaggat attcacggaa
    50701 tcctacaaat ttcatttaaa aatgctcaag acttggaaag agctgagcat ctcataacag
    50761 aaaataccga ctatacagtc gtcatcaaat aaggagaaga acatgtcaaa tatttacgat
    50821 agtgcaaacg aactcagtcg cggtctacgc ggattaccag aatacaaggc tgttaaagca
    50881 gctaaagatg cgattgcagc agatgctgag gcaagcaaaa tctttacaga atatcttgcc
    50941 tttcaagagg aaattcaaaa actagcacag acaggtcaaa tgccagatgc gtccttccaa
    51001 gctaagatgg aaggctttgg taaacagatt caaggaaata gcctcttgtc agaattcttt
    51061 accaagcaac aacaattggc catttacctt tctgacattg aaaaaattgt ttttgaacca
    51121 gtttcagaat tgctgaaata agaaaagaac tatcctaaag tcaggaattt ttaaggaatt
    51181 tctggctttt tatgataaaa taggtaaaag tattgcaagt atgaggtcca gtatgaaact
    51241 aaaaacaaac attcgccatt tacatggtag tatccgcgtc ccaggtgaca agtctatcag
    51301 ccaccgttcc attatctttg gaagtttggc tgagggtgag accaaggttt atgatattct
    51361 gcgaggtgaa gacgttcttt cgaccatgca ggtttttcgt gaccttggtg ttgaaattga
    51421 ggataaagat ggggttatta ccattcaagg tgtaggcatg gctggcttaa aagcgccaca
    51481 aaatgccctt aatatgggaa attctggcac ctcgattcgc ctgatttcag gtgtccttgc
    51541 tggtgcagat ttcgaagtag agatgtttgg agatgatagt ctttccaaac gtcctatgga
    51601 ccgtgtgacc cttccactga aaaaaatggg cgtcagcatt tcagggcaaa ctgaacgaga
    51661 cttgcctccc cttcgcttaa aagggacgaa aaacctaaga cctattcatt atgagttgcc
    51721 aattgcctct gcccaagtca agtcagcctt gatgtttgca gccttacaag ctaaggggga
    51781 gtcagttatt atcgaaaaag agtgcacccg taatcatact gaagatatgt tgaaacaatt
    51841 tggtggtcat ttaagtgtgg acggtaagaa aatcacagtc caagggccac aaaaattgac
    51901 aggacagaag gtggtcgtac caggagatat ttccagtgca gccttttggt tagtcgcagg
    51961 tttgattgtt ccaaattctc gtctagtgct gcagaatgtg gggatcaacg aaactcgcac
    52021 cggtattatt gatgtcattc gtgccatggg tggaaaattg gaaataactg aaatcgatcc
    52081 agtcgctaaa tcttctacct tgactgttga gtcttctgac ttgaaaggaa cagagattgg
    52141 tggcgctttg attccacgat tgattgatga attgcccatt attgccctgc ttgcgactca
    52201 agcccaaggt gtaacagtta tcaaggatgc tgaggagctc aaggtcaagg aaacagaccg
    52261 tattcaggtt gtggcagacg ccttaaatag tatgggagca gatattactc ctacggcaga
    52321 tgggatgatt atcaaaggaa aatcagctct tcacggtgct agagtcaata cgtttggtga
    52381 ccaccgtatc ggcatgatga cagctatcgc agccctattg gttgcagatg gagaggtgga
    52441 gcttgaccgt gcagaagcca tcaataccag ctatcctagt ttctttgatg atttggagag
    52501 cttgattcat ggctaaggtg ttattagggt ttatgggggc tggaaaatcg actattgcaa
    52561 gaggcttgga ccctaattac cttgatatgg atgctctgat tgagaagcgc ctaggtatgt
    52621 ccattgcgaa ttttttcgct gaaaagggag aagcagcctt tcgtcaggta gaatcagaag
    52681 tcctagctga tttactacaa acagaccaag tcgtgtcaac tggaggagga gtggttattt
    52741 ctcagagaaa tcgtgactta ctcaagacta atacagataa catctacctg aaagcagatt
    52801 ttgaaaccct ctacctacgt atcgtagctg ataaggacaa tcagcgaccg ctttttctaa
    52861 ataatagcaa ggaagaacta gtagctattt ttcaagaaag acaggcttgg tatgaggaag
    52921 tggctagtcg ggttttggat gtgaccaagc taagcccaga ggaaattata gaggaactaa
    52981 gatgaaaatt gcttatctag gtcccaaggg atcattttca caccacgttg tgcagacagc
    53041 ttttcctcat gaggaattgc aggcttttgc caacattaca gatgtcatca aggcttatga
    53101 gcaaggatta gtggactatt ctgtggtgcc agttgaaaat tctattgagg gtagtattca
    53161 tgaaaccttg gactatcttt ttcatcaggc tcacatccaa gcagtagcag aaatcgttca
    53221 gcctattcat cagcagttga tggtggttcc aggccatact aagattgaaa agattttttc
    53281 acatccacag gccttggctc aaggaaagaa attcatcgat gaacaatatc cagaggctca
    53341 aatcgaggta acagctagta cagcttatgc ggcccgtttt atttccgaac atccagatca
    53401 gccctttgca gcagttgcac ctagaagttc tgctgaagaa tatggattgg aactgattgc
    53461 cgaggatatt caggaaatgg aagccaattt cacacgtttc tggcttctag gagctgaaaa
    53521 gcctagtatt cccttgcaag cacaaactga aaagatgagt ttggccttga cattacctga
    53581 caaccttcca ggtgcacttt ataaggccct gtcgaccttt gcttggcgag gaattgactt
    53641 gacaaaaatt gaaagtcgtc cactcaagac agcactgggt gaatactttt tcattatcga
    53701 tgtggattat accgataagg acttggtcca ctttgcccaa aaagaattag aagcgattgg
    53761 aatccagtat aaaattctgg gtgcctatcc tatttatcca atatcagacc atggaaagga
    53821 gagaagatga gtaaagaaaa tcccttaagt catcatgagc agttgcgtta tgattatttg
    53881 ctaaaaaata ttcactacct caatgagaga gaaaaaaatg agtttgccta tttgcaagaa
    53941 aagctaactc ttgctagggg aaatagtagc tctagcttgg aacaagaaag agaagagcag
    54001 gttgacttac caagctatgc gaaccggagt cgctcacaat ccaaatcaca agcactctct
    54061 ttccctccaa aaaagaaaag acggaagctc cgtcttaaac gaatttttat ggtgattttt
    54121 tcacttttgg tctgtgtggc tttggccatg gtattcatgt ttttgcgtgg ttaccaagat
    54181 gctagcgcaa agaaaactgc tgatgcccgg gcagctcaag tagaagtctt taatggtcag
    54241 gacactagag atggagttaa tattttaatc atgggtactg atggtcgaat cggccagaac
    54301 agtgttgaga cacgaactga ctctattatg gtattaaatg tcgggggctc agataagaaa
    54361 atgaagctag tcagtttcat gcgtgataat ttggtctata tagatggtta tagtcaagtg
    54421 attaacggta gaaaacagac agataacaag ttaaacgtag cctacgagtt aggagaacaa
    54481 gaggggcaaa aaggggcaga aatggttcgt caagtcttga aagataattt tgacttggac
    54541 attaagtact acgctttggt tgatttccag gcctttgcca cagcgattga cacgcttttc
    54601 cctgatgggg tgacgattga tgctcaattt tcaacattga atgggcgtcc cctaacagaa
    54661 gctacagtcg gagatgattt atatgctacc gagactgagt ctccaaccca aaccattaaa
    54721 gtcggaaaac agcagatgaa tggctcgacc ctgctcaatt atgctcgttt ccgtgatgat
    54781 gatgaggcgg attacggccg tacaaaaaga cagcaacaag ttttaacagc aattttagag
    54841 caaattaaag atcccactaa acttttcact ggttctgaag ctcttggaaa ggtttttgct
    54901 atgacctcaa ccaacgtacc ttatactttc ctcctaacaa acggtttatc tgttttggat
    54961 ggagcaaaaa atggtattga aaaattgacg attccagaac tgggtgactg ggtagatgcc
    55021 tatgatgttt atggaggctt gggcttgctg gttgatcaaa acaaatatca aaccaagctc
    55081 gctcaaatgg gcttgagata agataatact caatgaaaat caaagtgcaa actaggaagc
    55141 tagccgcagg ctgctaaaaa cacagttttg aggttgtaga taagactgac gaagtcagtt
    55201 acctatagct acggcaaggt gaagctgacg tggtttgaag agattttcga agagtataag
    55261 acagaaaaat caaagtttga tagctatttg gatagcagtc aggctttgat tttttacagt
    55321 ctgcaataga ttttcacccc ttgtttgtgg tataatgaaa cgatacgata gaaagaataa
    55381 tgaacaatat gactgattta aaagcaattc aggctcgtag tctggagatg gctgaatatt
    55441 ttgtggcctt ttgcaaggaa catgatttac tttgctatct ctgtggggga ggcgctattg
    55501 gtgcccttcg aaacaagggg ttcattcctt gggacgacga cctagacttt tttatgcctc
    55561 gtaaagatta tgagaaatta gcagaattat ggcctcgtta tgcagatgaa cgttatttct
    55621 tgtcaaagag ttacaaggat tttgttgatc gtaatctttt tattaccatt cgtgacaagg
    55681 aaaccacctg tatcaagcct tatcagcagg atttggattt gccacatggt ctggccttgg
    55741 atgttttgcc tttggattat tatccgaaag atccagctga gcggaaaaaa caggttcgtt
    55801 gggccttgat ttattcactc ttttgtgcgc aaactattcc agaaaagcat ggtgctctca
    55861 tgaaatgggg aagtcgcatt ttactgggtt tgactccaaa atctctccgt tatcgcatct
    55921 ggaaaaaagc tgagaaagaa atgactaagt atgatttggc tgattgtgat ggcattacag
    55981 aattatgctc aggtcctggc tacatgagaa acaagtaccc aatcacatct tttgaagaca
    56041 atcttttctt gccatttgaa ggaacaaaga tgcctattcc aatcggctat gatgtctatc
    56101 tcagaactgc ttttggggat tatatgacgc ctccaccagc agacaagcag gtaccgcatc
    56161 atgatgttgt catcgctgat atggataagt cttatacaga atacaaggga gaatatggtg
    56221 gctaagaaaa aaatcttatt ttttatgtgg tctttttctc ttggaggtgg tgcagagaag
    56281 attctatcaa ccattgtttc aaatctggat ccagaaaagt atgatattga tattcttgaa
    56341 atggagcact ttgacaaggg atatgaatct gttccaaagc atgtacgcat tttaaaatcc
    56401 cttcaagatt atcgccaaac cagatggtta cgagcttttt tgtggagaat gagaatttat
    56461 tttccaagac tggctcgtcg tttgcttgta aaagatgatt atgatgttga agtttctttt
    56521 accattatga atccaccact gttgttctct aaaagaagag aagtcaagaa gatatcttgg
    56581 attcatggaa gtattgaaga acttcttaag gatagctcta aaagagaatc acatagaagc
    56641 cagttggatg ctgcgaatac aattgtaggg atttcaaaaa agaccagcaa ttctatcaag
    56701 gaagtttatc cagattatgc ttctaaatta cagacaatct acaatggata tgattttcag
    56761 actattctag aaaaatctca agagaagatc gatatcgaga ttgctcctca aagtatctgt
    56821 actatcggac ggattgagga aaataagggt tctgaccgtg tagtggaagt gatacgatta
    56881 ttacaccaag agggaaaaaa ctatcatctc tattttatcg gggctggtga tatggaagag
    56941 gaactgaaaa aacgagtcaa agagtatggg attgaggact atgtacattt ccttggttat
    57001 caaaaaaatc cttatcagta tctatctcag acgaaagttc ttttgtctat gtctaaacaa
    57061 gaaggttttc ctggagtgta tgtggaggcc ttgagtctgg gactcccttt tatctctacg
    57121 gacgttggag gggctgagga attatcccaa gaaggacgat ttggacaaat cattgagagc
    57181 aatcaagagg cagctcaggc gattactaat tacatgactt ctgcctcaaa ctttgatgtc
    57241 gatgaggcta gccaattcat tcaacaattt acaattacaa aacaaatcga acaagtagaa
    57301 aaactattag aggagtagca tggaaactgc attaattagt gtgattgtgc cagtctataa
    57361 tgtggcgcag tacctagaaa aatcgatagc ttccattcag aagcagacct atcaaaatct
    57421 ggaaattatt cttgttgatg atggtgcaac agatgaaagt ggtcgcttgt gtgattcaat
    57481 cgctgaacaa gatgacaggg tgtcagtgct tcataaaaag aacgaaggat tgtcgcaagc
    57541 acgaaatgat gggatgaagc aggctcacgg ggattatctg atttttattg actcagatga
    57601 ttatatccat ccagaaatga ttcagagctt atatgagcaa ttagttcaag aagatgcgga
    57661 tgtttcgagc tgtggtgtca tgaatgtcta tgctaatgat gaaagcccac agtcagccaa
    57721 tcaggatgac tattttgtct gtgattctca aacatttcta aaggaatacc tcataggtga
    57781 aaaaatacct gggacgattt gcaataagct aatcaagaga cagattgcaa ctgacctatc
    57841 ctttcctaag gggttgattt acgaagatgc ctattaccat tttgatttaa tcaagttggc
    57901 caagaagtat gtggttaata ctaaacccta ttattactat ttccatagag gggatagtat
    57961 tacgaccaaa ccctatgcag agaaggattt agcctatatt gatatctacc aaaagtttta
    58021 taatgaagtt gtgaaaaact atcctgactt gaaagaggtc gcttttttca gattggccta
    58081 tgcccacttc tttattctgg ataagatgtt gctagatgat cagtataaac agtttgaagc
    58141 ctattctcag attcatcgtt ttttaaaagg ccatgccttt gctatttcta ggaattcaat
    58201 tttccgtaag gggagaagaa ttagtgcttt ggccctattc ataaatattt ccttataccg
    58261 attcttatta ctgaaaaata ttgaaaaatc taaaaaatta cattagaacg ggggtgacca
    58321 agtgattgat gggaaacgat tattatttag tttgaccata gtcagttatg ccttgacgct
    58381 agtaagtgga attgtgtatc tgtttaataa taataatgtt agcttacttt ctactttatt
    58441 gttcttgttg gttagtagct tgattgcttg ttggaatgat atcaagtatt acttaatcca
    58501 ttttattttc tatttaacca tttttgtatt tctggtatca agaccgacca ttgattattt
    58561 tagggatggt gctttggata cctatcatcc aatagcctat cgttttgcct ttatagttgt
    58621 catggtttcg attctgggct tgacaacagg aggcgtcctg gctcgttact tcatagctag
    58681 gaagaaaata aaagtagcaa atataggaaa ttctctaaaa gaggtttata tcaagcggtt
    58741 acgctttgta tcactaggag tttttcttct aacttatcct ttctatttca ttcggttatt
    58801 tgaacggctc ttgtatcgtt tgcagacttc ctactatgcc tactatgcaa attttgaaag
    58861 taaactgcct tattttacct acattttgtc tacctttacg gtctatgcaa tgtgtatgta
    58921 tctggcaacc aagccaaaga aattgcaggc cacagcagtg cttgtctcct ttattgcagc
    58981 taacactatt catttggcaa ttgggacacg aaatcccttt attttaagta ttttatttgc
    59041 ttttgtttat tactttatgc gggagcaagc tgaaaaagga aaatggattg ggtttaaaga
    59101 aaagttagcg atttttgtag gttctcctat tctcatgtta gcgatgggag tactcaatta
    59161 tgtacgggat aatgtccaag tttcccatac aggtttctgg gatatcttac ttgactttat
    59221 ctataaacaa gggactagtt ttggtgttct ggctcgaggt tttctattta acagtagcct
    59281 cccttaccga gatttccgta attttacttt tggtcctgtt cttgattatt ttgcaagggg
    59341 gagtttggga gccattttcg gaggaaaagc ctttgaacat acaaccaata gtgtggaact
    59401 agctattgat agtaatagtt atgcccacaa tctatcctat cttgtcttga acaaggaata
    59461 cctgaaaggg catggtatcg gaagtagtta tatcatggag ttgtataccg actatggtat
    59521 gattggagtc tttctgctta gtttcttact cggcgtatta tttatagcca tgctgcaagt
    59581 agcctatcgc tcaaggacaa tcctatttgc tttatcccta ctcatcttga ataatctatt
    59641 ctttatgcca agaagcagct tttcagaaag tttcttcaat ttatttacaa tgcaattctg
    59701 gggaattgtt cttgtgatta tatttgtagc aaaaatgctt acaaaggaaa actagtatct
    59761 actaaacaaa ggagaaaaaa atcatgtttg aacattattc agtagctgat ttgtttgcaa
    59821 atctttataa aaaacgaaaa gcaaatattt tagctctcat cgctttattt gctctcattg
    59881 ctgtaccatt tacaattaaa gcagttagga ataaaaacac tgtcaaagat acaacaagtt
    59941 attcaactta tcttatctat aaaatcactc ctccagaaga ttcggccaaa acgattttga
    60001 atcatcaaat tggtggttat agtgattttt atgggaaatt gattgatggt aatttgaatg
    60061 gagcttatct tttcaatgat gtagaaccca gtgagttgaa aaaaattgcc agtgaattag
    60121 acacgacgga aacaaccttg aaaaattcaa cgaatgacta ttggtggaaa aaattgaccg
    60181 tctactatat gattgacgat gcaggggttg gtgtgaaaat tttgacatca agtaaagatg
    60241 ccaatgactt gttagagaaa aaaattgatg ggttgattga gaaatttaaa catgcttatg
    60301 caaatgtgaa aattgaaaaa ctggaaacca tcaactctaa agaattgaac gcaaatggtg
    60361 aaacagcgct tggcttaaat gtgaaaaatc tgattcttcg tttagttgtt attggagtgg
    60421 tttgtgtgat tttggttgtg atgggaaatg tgttagttta tctctttaat ccaacaatca
    60481 atagagtagg tgatttttct cagtatcaaa ttgattttgt aacagagatt acaacaattg
    60541 ctaacctagc agatgttttg tcatacaaaa atactggaca ggaattgacc atcgttagct
    60601 caaataaagc tatcctagat aaattgaaac agagtcaaga agctttaaaa ggaatgcatt
    60661 ttgtagattt gcaggatgta tcatctcttt tggaaagaga tacagtcctt cttgttgaag
    60721 agtacggagt gactcgttat aagaaatttg agcaaagtct tcaaattctc agaaacttaa
    60781 atcgttctat cctcggtgta gcaaccttta aattataagt attctgatgt ataaggtctt
    60841 caaaatcggc tcttagccga ttttgaaggc tttttctatc tgtctgcaga aattttctct
    60901 tggagtctcc tatagaaaga tcctagggaa aatctatccg tatagaggag ccttcttttg
    60961 ataaaatcgt aaaaatctag tataatagat agaactgaaa gtatgaggtt actagcaatg
    61021 aaaatgaaac aaattagtga tacaacttta aaaatcacga tgtctttaga ggatttgatg
    61081 gatcgtggaa tggagattgc tgactttctc gttcctcaag aaaaaacaga agagttcttt
    61141 tatgctatct tggatgagct agagatgcct gatagctttc tggatacagg catgttgagc
    61201 ttccgtgtga ctccaaaacc tgataaggta gatgtctttg tgaccaagtc aaagattgac
    61261 caaaatctag attttgaaga cttatcggat ttgccagata tggaagaatt ggctcaaatg
    61321 tctccagatg aatttatcaa aaccctggaa aaaagcatcg cagacaaaac caaggatgat
    61381 atcgaagcca ttcaatctct tgagcaagtt gaagccaagg aagaagagca agagcaggct
    61441 gaacaagaag ctgagagtaa gaaagaacct tacatctact acatcctttc ttttgctaag
    61501 ttggctgact tggtagcttt tgccaagaca gtgacttttg aaatggaaac ttctgaactc
    61561 tacaaaatga acgagcgcta ttatttgacc attttagtgg atattgaaaa tcatccaagc
    61621 ccatatccag cttggctgtt ggcccgtatg cgcgagtttg cagacgatag tgatatcagt
    61681 cgctcagtct tacaagagta tggtcaagtc ttgatgagtc acgatgcagt gctcaatctg
    61741 caaaaaatcg gctaatcctt tctggaaagc tattttcaga ttttaagaag agcgaatcgt
    61801 ctgattcgtt ttttcttttt atactgaaat agtgatttac tataatagga attttcacaa
    61861 aattctgtta taatggctat attagaaaat ttcgaggaga taaagatgac agttaaaatt
    61921 gctttactag gatttggtac cgttgcaagt ggtgtgcctt tcctcctaaa ggaaaatgga
    61981 ggaaaaatca atcaatcagc acattcagat atcaaagttg ctaaggtatt ggtcaaggat
    62041 gaagatgaaa aaaatcgctt gcttgcagca gggaatgact ttaactttgt aaccaatgtg
    62101 gatgatattt tatcagacca ggatattact atcgtagtgg aattgatggg gcgtattgag
    62161 cctgctaaaa cctttatcac tcgtgccttg gaagctggaa aacacgttgt tactgctaac
    62221 aaggaccttt tagctgtcca tggcgcagaa ttgctagaaa tcgctcaagc taacaaggta
    62281 gcactttact acgaagcagc agttgctggt gggattccaa ttcttcgtac tttagcaaat
    62341 tccttggctt ctgataaact tacgcgcgtg cttggagtag tcaacggaac ttccaacttc
    62401 atggtgacca agatggtgga agaaggctgg tcttacgatg atgctcttgc ggaagcacaa
    62461 cgtctaggat ttgcagaaag cgatccgacg aatgacgtag atgggattga tgcagcctac
    62521 aagatggtta ttttgagcca atttgccttt ggcatgaaga ttgcctttga tgatgtagcc
    62581 cacaagggaa tccgcaatat cacaccagaa gacgtagctg tagctcaaga gcttggttac
    62641 gtagtgaaat tggttggttc tattgaggaa acttcttcag gtattgctgc agaagtgact
    62701 ccaaccttcc tacctaaagc gcacccactt gctagtgtga atggcgtaat gaacgctgtc
    62761 tttgtagaat ctatcggtat tggtgagtct atgtactacg gaccaggtgc gggtcaaaaa
    62821 ccaactgcaa caagtgttgt agctgatatt gtccgtatcg ttcgtcgttt gaatgatggt
    62881 accattggca aagacttcaa cgaatatagc cgtgacttgg tcttggcaaa tcctgaagat
    62941 gtcaaagcaa actactattt ctcaatcttg gctccagact caaaaggtca ggtcttgaag
    63001 ttggctgaaa tcttcaatgc tcaagatatt tcctttaagc aaatccttca agatggcaaa
    63061 gagggtgaca aggcgcgtgt cgttatcatc acacacaaga ttaataaagc ccagcttgaa
    63121 aatgtctcag ctgaattgaa gaaggtttca gaattcgacc tcttgaatac cttcaaggtg
    63181 ctaggagaat aagatgaaga ttattgtacc tgcaaccagt gccaatatcg ggccaggttt
    63241 tgactcggtc ggtgtagctg taaccaagta tcttcaaatt gaggtctgcg aagaacgaga
    63301 tgagtggctg attgaacacc agattggcaa atggattcca catgatgagc gtaatctctt
    63361 gctcaaaatc gctttgcaaa ttgtaccaga cttgcaacca agacgcttga aaatgaccag
    63421 tgatgtccct ttggcgcgcg gtttgggttc ttccagctcg gttatcgttg ctgggattga
    63481 actagccaac caactgggtc aactcaactt atcagaccat gaaaaattgc agttagcgac
    63541 caagattgaa gggcatcctg acaatgtggc tccagccatt tatggtaatc tcgttattgc
    63601 aagttctgtt gaagggcaag tctctgctat cgtagcagac tttccagagt gtgattttct
    63661 agcttacatt ccaaactatg aattacgtac tcgcgacagc cgtagtgtct tgcctaaaaa
    63721 attgtcttat aaggaagctg ttgctgcaag ttctatcgcc aatgtagcgg ttgctgcctt
    63781 gttggcagga gacatggtga ccgctgggca agcaatcgag ggagacctct tccatgagcg
    63841 ctatcgtcag gacttggtaa gagaatttgc gatgattaag caagtgacca aagaaaatgg
    63901 ggcctatgca acctaccttt ctggtgctgg gccgacagtt atggttctgg cttctcatga
    63961 caagatgcca acaattaagg cagaattgga aaagcaacct ttcaaaggaa aactgcatga
    64021 cttgagagtt gatacccaag gtgtccgtgt agaagcaaaa taaagaatag aatataggat
    64081 ggggaaactc ttgaccagag gggttcatat cctttttgtg aaaagaagtt tatactcaat
    64141 gaaaatcaaa gagcaaacta ggaagctagc cgcaggctgc tcaaaacagt gttttgaggt
    64201 tgtggataga actgacgacg tcagctcaag acactgtttt gaggttgcag atagaactga
    64261 cgaagtcagt aacatatata cggtaaggtg acgctgacgt ggtttgaaga gattttcgaa
    64321 gagtattagt taaaaacgtg ataaaggaga aataaaaatg gcagaaattt atctagcagg
    64381 tggttgtttt tggggcctag aggaatattt ttcacgcatt tctggagtgc tagaaaccag
    64441 tgttggctac gctaatggtc aagtcgaaac gaccaattac cagttgatca aggaaacaga
    64501 ccatgcagaa acggtccaag tgatttacga tgagaaggaa gtgtcactca gagagatttt
    64561 actttattat ttccgagtta tcgatcctct atctatcaat caacaaggga atgaccgtgg
    64621 tcgccaatat cgaactggga tttattatca ggatgaagca gatttgccag ctatctacac
    64681 agtggtacag gagcaggaac gcatgctggg tcgaaagatt gcagtagaag tggagcaatt
    64741 acgccactac attctggctg aagactacca ccaagattat ctcaggaaga atccttcagg
    64801 ttactgtcat atcgatgtga ccgatgctga taagccattg attgatgcag caaactatga
    64861 aaagcctagt caagaggtgt tgaaggccag tctatctgaa gagtcttatc gtgtcacaca
    64921 agaagctgct acagaagctc catttaccaa tgcctatgac caaacctttg aagaggggat
    64981 ttatgtagat attacgacag gtgagccact cttttttgcc aaggataagt ttgcttcagg
    65041 ttgtggttgg ccaagtttta gccgtccgat ttccaaagag ttgattcatt attacaagga
    65101 tctgagccat ggaatggagc gaattgaagt tcgttctcgt tcaggcagtg ctcacttggg
    65161 tcatgttttc acagatggac cgcgggagtt aggcggcctc cgttactgta tcaattctgc
    65221 ttctttacgc tttgtggcca aggatgagat ggaaaaagca ggatatggct atctattgcc
    65281 ttacttaaac aaataaaaca gagagtgggg cttcccactt tcttcatttc tagaatatga
    65341 atagaaggga tttatgaaac acctattatc ttacttcaaa ccctacatca aggaatcaat
    65401 tttagccccc ttgttcaagc tgttagaagc tgtttttgag ctcttgcttc caatggtgat
    65461 tgctgggatt gttgaccaat ctttacctca gggagatcaa ggtcatctct ggatgcagat
    65521 tggcctgctc cttatctttg cagtaattgg cgttttagtg gccttgatag ctcaatttta
    65581 ctcagcaaag gcagcagtag gttttgctaa ggaattgaca aacgatcttt atcgtcatat
    65641 tctttccttg cccaaggaca gcagagaccg tctgacaaca tctagtttgg tcactcgctt
    65701 gacttcggat acctaccaga ttcagactgg tatcaatcaa ttcctgcgtc tctttttgcg
    65761 agcgcccatt atcgtttttg gtgccatttt tatggcttat cgaatctcaa ctgagttgac
    65821 tttctggttc ttagtcatgg ttgccatttt gaccattgtc attgtagggg tatctcgatt
    65881 ggtcaatcct ctctacagta gtctcagaaa gaaaacggac caactggttc aggaaacgcg
    65941 ccagcaattg caagggatgc gggttattcg tgcttttggt caagaaaaac gagagttaca
    66001 gatttttcaa acccttaacc aagtttatgc tagattacaa gaaaagacag gtttctggtc
    66061 tagtttatta acacctctga cctatctgat tgtcaatgga actcttctcg ttattatctg
    66121 gcaaggctat atttcaattc aaggaggagt gctcagtcaa ggtgctctca ttgctcttat
    66181 caattacctc ttacagattt tggtggaatt ggtcaagcta gccatgttga taaattctct
    66241 caaccagtcc tatatctcag ccaagcgaat cgaggaagtc tttgttgagg ctccagagga
    66301 tatccattca gagttagaac aaaagcaagc taccagagat aaggttttac aagtccaaga
    66361 attgaccttt acctatcctg atgcggccca gccttctctg agagacattt cctttgatat
    66421 gactcaagga caaatccttg gtatcattgg ggggactggt tctggtaaat caagcttggt
    66481 gcaactctta cttggacttt atccagtaga caaggggaac attgaccttt atcaaaatgg
    66541 acgtagtcct cttaatttgg agcagtggcg gtcttggatt gcctatgtac ctcaaaaggt
    66601 cgaactcttt aaaggaacca ttcgttccaa cttgactcta ggtttcaatc aagaagtatc
    66661 tgaccagaaa ctctggcagg ccttggagat tgcgcaagct aaggattttg tcagtgaaaa
    66721 ggaaggactc ttggatgtcc tagttgaggc aggggggcga aatttctcag gtggacaaaa
    66781 acaaaggttg tctatcgccc gagcagtctt gcgccaagct ccgtttctca tcctagatga
    66841 tgcaacctcg gcactggata ccattacaga gtccaagctc ttgaaagcta ttagagaaaa
    66901 ttttccaaac acgagcttaa ttttgatctc tcaacgaacc tcaactttac agatggcgga
    66961 ccagattctc ctcttggaaa aaggtgagtt gctagctgtt ggcaagcacg atgacttgat
    67021 gaaatccagc caagtctatc gtgaaatcaa tgcatcccaa catggaaagg aggactagaa
    67081 tgaaacgaca aactgtaaac cagacgctca aacgtttagc cgtagattta gcaaaccatc
    67141 ccttcctcct tttcctagcc tttctaggaa ctattgccca agttggctta tcaatttacc
    67201 tacctattct gattgggcag gtcattgacc aagtcctagt ggctggttca tcaccagttt
    67261 tttggcagat ttttctccag atgctcttgg tggtaatagg aaatactctg gtacaatggg
    67321 ccaatcctct cctctataat cgtctaatct tctcttatac cagagattta cgggagcgaa
    67381 tcatccataa gctccatcgt ttaccgattg cctttgtaga taggcaaggt agtggagaga
    67441 tggttagtcg tgtaaccatg gacatcgaac agttggcagc tggcttgacc atgattttta
    67501 accaattttt cattggtgtt ttgatgattt tggttagtat tctagccatg ctccaaattc
    67561 atctcctcat gactctctta gtcttgctgt tgacgccact gtccatggtg atttcacgct
    67621 ttattgccaa gagatcctat catctcttcc agaagcaaac agagacgagg ggaattcaga
    67681 ctcagttgat tgaagaatcg cttagccagc agactataat ccagtccttc aatgctcaaa
    67741 cagaatttat ccaaagattg cgtgaggctc atgacaacta ctcaggctat tctcagtcag
    67801 ccatctttta ttcttcaacg gtcaatcctt cgactcgctt tgtaaatgca ctcatttatg
    67861 cccttttagc tggagtagga gcttatcgta tcatgatggg ttcaaccttg accgtcggtc
    67921 gtttagtgac ttttttgaac tatgttcagc aatacaccaa gccctttaat gatatttctt
    67981 cagtgctagc tgagttgcaa agtgctctgg cttgcgtaga gcgtatctat ggagtcttag
    68041 atagccctga agtggctgaa acaggtaagg aagtcttgac cagtgaccaa gttaagggag
    68101 ctatttcctt taaacatgtc tcttttggct accatcctga aaaaattttg attaaggact
    68161 tgtctatcga tattccagct ggtagtaaga tagccatcgt tggtccgaca ggtgctggaa
    68221 aatcaactct tatcaatctc cttatgcgtt tttatcccat tagctcggga gatatcttgc
    68281 tggatgggca atccatttat gattatacac gagtatcatt gagacagcag tttggtatgg
    68341 tgcttcaaga aacctggctc acacaaggga ccattcatga taatattgcc tttggcaatc
    68401 ctgaagccag tcgagagcaa gtaattgctg ctgccaaagc agctaatgca gactttttca
    68461 tccaacagtt gccacaggga tacgatacca agttggaaaa tgctggagaa tctctctctg
    68521 tcggccaagc tcagctcttg accatagccc gagtctttct ggctattcca aagattctta
    68581 tcttagacga ggcaacttct tccattgata cacggacaga agtgctggta caggatgcct
    68641 ttgcaaaact catgaagggc cgcacaagct tcatcattgc tcaccgtttg tcaaccattc
    68701 aggatgcgga tttaattctt gtcttagtag atggtgatat tgttgaatat ggtaaccatc
    68761 aagaactcat ggatagaaag ggtaagtatt accaaatgca aaaagctgcg gcttttagtt
    68821 ctgaataagc cattctcttt tgaaagttta tggacgaaaa aagttgcctt cgagtgactt
    68881 ttttgttaca atagctagaa aaattgttta ctgtaatact caatgaaaat caaagagcaa
    68941 actaggaagc tagccgtagg t
//
LOCUS       DAVDWT010000007        68580 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVDWT010000007 DAVDWT010000000
VERSION     DAVDWT010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 68580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68580
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.007"
                     /strain="398"
                     /db_xref="taxon:1313"
     misc_binding    complement(215..444)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(656..1657)
                     /locus_tag="VUW50_000543"
     CDS             complement(656..1657)
                     /locus_tag="VUW50_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7982495.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            complement(1765..2133)
                     /locus_tag="VUW50_000544"
     CDS             complement(1765..2133)
                     /locus_tag="VUW50_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982496.1"
                     /translation="MKDYKINFDLGKIEYFDNNCLIQVYKFISFYDICEMVFAFHLPP
                     DELITNVIFKEKINSMLKCYIDRLLYVFINPTHFTEKVNLQFYGSFFSYEFICREVGN
                     ILKNKGVKCNLNFFEGEEYL"
     gene            complement(2229..2939)
                     /locus_tag="VUW50_000545"
     CDS             complement(2229..2939)
                     /locus_tag="VUW50_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET7982497.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            complement(2942..4633)
                     /locus_tag="VUW50_000546"
     CDS             complement(2942..4633)
                     /locus_tag="VUW50_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET7982498.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            complement(4645..5655)
                     /locus_tag="VUW50_000547"
     CDS             complement(4645..5655)
                     /locus_tag="VUW50_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982499.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            complement(5758..6825)
                     /locus_tag="VUW50_000548"
     CDS             complement(5758..6825)
                     /locus_tag="VUW50_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7982500.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            complement(6850..7509)
                     /locus_tag="VUW50_000549"
     CDS             complement(6850..7509)
                     /locus_tag="VUW50_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HET7982501.1"
                     /translation="MKNLTKIKFKENGEFNHFSGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            complement(7689..8699)
                     /gene="galE"
                     /locus_tag="VUW50_000550"
     CDS             complement(7689..8699)
                     /gene="galE"
                     /locus_tag="VUW50_000550"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET7982502.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSKLVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWNWQSKKPNG
                     YN"
     gene            complement(8712..10187)
                     /gene="galT"
                     /locus_tag="VUW50_000551"
     CDS             complement(8712..10187)
                     /gene="galT"
                     /locus_tag="VUW50_000551"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HET7982503.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGIDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYSYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFCFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            complement(10200..10841)
                     /locus_tag="VUW50_000552"
     CDS             complement(10200..10841)
                     /locus_tag="VUW50_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HET7982504.1"
                     /translation="MLRLYLENEIIELSNNLETIWVSVLDKYEKLFEYLSNEERIELI
                     KEDLIINESVLNVDKKGYELICLQHPVSYDLRRIISVIKISTDIERIGDRIVEILKNL
                     QIIQNNEILKKIISEIKILHEVIGLHMNRAISCYREEQSGCLDMVVIQKQNEIEELSI
                     NMEKKIMNYIFEDDGNVSEVISALDIIHHLDKIAHTTQAIYKWIMYRKYGNIN"
     gene            complement(10899..11111)
                     /locus_tag="VUW50_000553"
     CDS             complement(10899..11111)
                     /locus_tag="VUW50_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982505.1"
                     /translation="MDERFLDHAIINQIDVTSEQIVLMSDGFYEFYQNNQNKTFEELI
                     KMRFNSSAIDPIYGKKDDASIVVIDV"
     gene            complement(11454..11750)
                     /locus_tag="VUW50_000554"
     CDS             complement(11454..11750)
                     /locus_tag="VUW50_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982506.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYNKMK"
     gene            complement(11734..13893)
                     /gene="pfbA"
                     /locus_tag="VUW50_000555"
     CDS             complement(11734..13893)
                     /gene="pfbA"
                     /locus_tag="VUW50_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HET7982507.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPIEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(15024..16250)
                     /locus_tag="VUW50_000556"
     CDS             complement(15024..16250)
                     /locus_tag="VUW50_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HET7982508.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(16293..16985)
                     /locus_tag="VUW50_000557"
     CDS             complement(16293..16985)
                     /locus_tag="VUW50_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET7982509.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(17158..18909)
                     /locus_tag="VUW50_000558"
     CDS             complement(17158..18909)
                     /locus_tag="VUW50_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982510.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTAMVSAGRVFALIDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDDQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(18899..20644)
                     /locus_tag="VUW50_000559"
     CDS             complement(18899..20644)
                     /locus_tag="VUW50_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982511.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLVVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGTHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(20772..21146)
                     /locus_tag="VUW50_000560"
     CDS             complement(20772..21146)
                     /locus_tag="VUW50_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HET7982512.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYEKEKP
                     GVLYSSKISSNRVNVALPYVGY"
     gene            complement(21299..21755)
                     /locus_tag="VUW50_000561"
                     /pseudo
     CDS             complement(21299..21755)
                     /locus_tag="VUW50_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            21889..22716
                     /gene="xth"
                     /locus_tag="VUW50_000562"
     CDS             21889..22716
                     /gene="xth"
                     /locus_tag="VUW50_000562"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET7982513.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDTKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            22728..22865
                     /locus_tag="VUW50_000563"
     CDS             22728..22865
                     /locus_tag="VUW50_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET7982514.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEYQRPEENFLLKSV
                     N"
     gene            22865..23104
                     /locus_tag="VUW50_000564"
     CDS             22865..23104
                     /locus_tag="VUW50_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET7982515.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVSKLYQKVMEANYPI
                     YRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      23487..23582
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23982..24563
                     /locus_tag="VUW50_000565"
     CDS             23982..24563
                     /locus_tag="VUW50_000565"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HET7982516.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            24563..25825
                     /locus_tag="VUW50_000566"
     CDS             24563..25825
                     /locus_tag="VUW50_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HET7982517.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPIP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(25876..26337)
                     /locus_tag="VUW50_000567"
     CDS             complement(25876..26337)
                     /locus_tag="VUW50_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HET7982518.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(26334..27098)
                     /locus_tag="VUW50_000568"
     CDS             complement(26334..27098)
                     /locus_tag="VUW50_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HET7982519.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(27279..27683)
                     /locus_tag="VUW50_000569"
     CDS             complement(27279..27683)
                     /locus_tag="VUW50_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET7982520.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGKNVCKATFTMFVTGQRSEDRQVRI"
     gene            complement(27753..29234)
                     /locus_tag="VUW50_000570"
     CDS             complement(27753..29234)
                     /locus_tag="VUW50_000570"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HET7982521.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAAAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARTNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEHVGISLD"
     gene            complement(29253..30431)
                     /locus_tag="VUW50_000571"
     CDS             complement(29253..30431)
                     /locus_tag="VUW50_000571"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET7982522.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKREDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDIVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            30595..31602
                     /gene="galR"
                     /locus_tag="VUW50_000572"
     CDS             30595..31602
                     /gene="galR"
                     /locus_tag="VUW50_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HET7982523.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGINLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(31649..32686)
                     /locus_tag="VUW50_000573"
     CDS             complement(31649..32686)
                     /locus_tag="VUW50_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7982524.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAAFECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(32687..33040)
                     /locus_tag="VUW50_000574"
     CDS             complement(32687..33040)
                     /locus_tag="VUW50_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7982525.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(33284..34174)
                     /locus_tag="VUW50_000575"
     CDS             complement(33284..34174)
                     /locus_tag="VUW50_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET7982526.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILNLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALSRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            34307..34843
                     /locus_tag="VUW50_000576"
     CDS             34307..34843
                     /locus_tag="VUW50_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7982527.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(35009..35815)
                     /locus_tag="VUW50_000577"
     CDS             complement(35009..35815)
                     /locus_tag="VUW50_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HET7982528.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(36160..37680)
                     /locus_tag="VUW50_000578"
     CDS             complement(36160..37680)
                     /locus_tag="VUW50_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HET7982529.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLAALIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVTV
                     LLGLSYSPALLVQKEKENVIIAGKIGPEPEILANMYKLLIEENTSLTATVKPNFGKTT
                     FLYEALKSGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVSTLEPALRYQAIQSGEIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELETVLNKLAGKITESQMSQLNYQVGVEGKPAEQVAKEFLQEQGLLKK"
     gene            complement(37673..38401)
                     /locus_tag="VUW50_000579"
     CDS             complement(37673..38401)
                     /locus_tag="VUW50_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982530.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            complement(38416..39180)
                     /locus_tag="VUW50_000580"
     CDS             complement(38416..39180)
                     /locus_tag="VUW50_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7982531.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(39184..39627)
                     /locus_tag="VUW50_000581"
     CDS             complement(39184..39627)
                     /locus_tag="VUW50_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7982532.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(40309..40605)
                     /locus_tag="VUW50_000582"
     CDS             complement(40309..40605)
                     /locus_tag="VUW50_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET7982533.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(40627..41691)
                     /gene="pepA"
                     /locus_tag="VUW50_000583"
     CDS             complement(40627..41691)
                     /gene="pepA"
                     /locus_tag="VUW50_000583"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HET7982534.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKDEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            42092..42793
                     /locus_tag="VUW50_000584"
     CDS             42092..42793
                     /locus_tag="VUW50_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HET7982535.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRATVD
                     NRASFKELDVYDASALKAYLEENQIDAVLHYAGEIVVSESIENPSKYFTANVAGRNQV
                     LKVLSEVGTQKIMFSSTASLYGNNCIDKPATEDTLIDPVNPYTETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            43216..44175
                     /locus_tag="VUW50_000585"
     CDS             43216..44175
                     /locus_tag="VUW50_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7982536.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            44165..45121
                     /locus_tag="VUW50_000586"
     CDS             44165..45121
                     /locus_tag="VUW50_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HET7982537.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQNLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQSLIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            45118..45870
                     /locus_tag="VUW50_000587"
     CDS             45118..45870
                     /locus_tag="VUW50_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7982538.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            45966..46931
                     /locus_tag="VUW50_000588"
     CDS             45966..46931
                     /locus_tag="VUW50_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7982539.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEITI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLAGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTTFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(47156..47398)
                     /gene="yidD"
                     /locus_tag="VUW50_000589"
     CDS             complement(47156..47398)
                     /gene="yidD"
                     /locus_tag="VUW50_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET7982540.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(47398..48120)
                     /locus_tag="VUW50_000590"
     CDS             complement(47398..48120)
                     /locus_tag="VUW50_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET7982541.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(48107..48676)
                     /locus_tag="VUW50_000591"
     CDS             complement(48107..48676)
                     /locus_tag="VUW50_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HET7982542.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(48695..49423)
                     /locus_tag="VUW50_000592"
     CDS             complement(48695..49423)
                     /locus_tag="VUW50_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET7982543.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(49423..50163)
                     /gene="xerD"
                     /locus_tag="VUW50_000593"
     CDS             complement(49423..50163)
                     /gene="xerD"
                     /locus_tag="VUW50_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET7982544.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQRRIVT
                     IPTALLSELEPLMGQTYLFERGGKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(50154..50615)
                     /locus_tag="VUW50_000594"
     CDS             complement(50154..50615)
                     /locus_tag="VUW50_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7982545.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(50612..51133)
                     /locus_tag="VUW50_000595"
     CDS             complement(50612..51133)
                     /locus_tag="VUW50_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7982546.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(51109..52080)
                     /locus_tag="VUW50_000596"
     CDS             complement(51109..52080)
                     /locus_tag="VUW50_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HET7982547.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(52077..52871)
                     /gene="racE"
                     /locus_tag="VUW50_000597"
     CDS             complement(52077..52871)
                     /gene="racE"
                     /locus_tag="VUW50_000597"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET7982548.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            complement(53078..53326)
                     /locus_tag="VUW50_000598"
     CDS             complement(53078..53326)
                     /locus_tag="VUW50_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HET7982549.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(53582..55207)
                     /gene="treC"
                     /locus_tag="VUW50_000599"
     CDS             complement(53582..55207)
                     /gene="treC"
                     /locus_tag="VUW50_000599"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HET7982550.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTGNGIGDFRGIIGKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCIGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKYINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(55385..57352)
                     /gene="treP"
                     /locus_tag="VUW50_000600"
     CDS             complement(55385..57352)
                     /gene="treP"
                     /locus_tag="VUW50_000600"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HET7982551.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFTYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFCKAGLFTKTEGDTNLQAEFVAQEEAEFVSHEPVELTSVEII
                     SPLTGQVKELSQATDPVFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATITGTYPTTIQAGASLMVATRI"
     gene            57537..58247
                     /gene="treR"
                     /locus_tag="VUW50_000601"
     CDS             57537..58247
                     /gene="treR"
                     /locus_tag="VUW50_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HET7982552.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            complement(58280..59083)
                     /locus_tag="VUW50_000602"
     CDS             complement(58280..59083)
                     /locus_tag="VUW50_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7982553.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQDPKMGTASSLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(59096..59989)
                     /locus_tag="VUW50_000603"
     CDS             complement(59096..59989)
                     /locus_tag="VUW50_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7982554.1"
                     /translation="MNFVLSSLSEGLLWSIVAIGDYLTFRILDIADMTAEGAFPLGAA
                     VVVSQIQAGANPWLATLLALLAGMVAGLVSGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGAVFSLSLVCFLLVCLVLTLLMKTEIGLVL
                     RSTGDNIPMSEANGVNVDTMKIVGYMISNGLIALCGSLFAQNDGFSDVTSGTGTIVVG
                     LSSVIIAEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYEIPNLDQNLVRLFNAILLTL
                     VLFAPELQKRLKIRGLKLRNE"
     gene            complement(60001..60861)
                     /locus_tag="VUW50_000604"
                     /pseudo
     CDS             complement(60001..60861)
                     /locus_tag="VUW50_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813348.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(61429..62355)
                     /locus_tag="VUW50_000605"
     CDS             complement(61429..62355)
                     /locus_tag="VUW50_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982555.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            complement(62366..63433)
                     /locus_tag="VUW50_000606"
     CDS             complement(62366..63433)
                     /locus_tag="VUW50_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982556.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGELYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            complement(63442..64368)
                     /locus_tag="VUW50_000607"
     CDS             complement(63442..64368)
                     /locus_tag="VUW50_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7982557.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            complement(64368..65864)
                     /locus_tag="VUW50_000608"
     CDS             complement(64368..65864)
                     /locus_tag="VUW50_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7982558.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            complement(65931..67910)
                     /locus_tag="VUW50_000609"
     CDS             complement(65931..67910)
                     /locus_tag="VUW50_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7982559.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
BASE COUNT        20612 a        15101 c        12103 g        20764 t
ORIGIN      
        1 ttttgagcaa cctgcggcta gtttcttagt ttgctctttg attttcattg agtattacta
       61 gcttttttcg tattagtcca gcctttttgt ttgcttttag tagtaggcaa ggagctgtag
      121 atagaactca agttcatcaa agcgacttaa ggccctaata aaagataaac caaacgacgg
      181 atagaaaaaa gcccacacac agaatatact tccgtgtgag ggcgttggta acgcggtgcc
      241 acctcaatta taaagggact atccctttac atctctgcct tgtttaacaa caagctgcac
      301 tgtaaggtgt gcgcaccgaa ttttcattgt ttcaaattca ttttcaaaat cagcccactt
      361 tcactacttc caaccaccta ttcacaacca ccacaggctc cctgaagatc aaaaatagtt
      421 acttttctga tttgttgaac ttattttaat actttgtttt ttctttgtca agactttttt
      481 acgatttttt tgaaaatatc attcgaatgt gaccatgtct tccttagatc gaacatgaac
      541 atgtcccact tgttagaaat tggatccaac tcaatagaaa ctgaatggag gctaaacaga
      601 acttatttta gaacactcca tcttttccac taggattttc aagaattaaa caatactaga
      661 aactctgtct cctaacaaat ttaggagaaa cttcaacaga tgtgacactt tcccctttaa
      721 taattgctaa aacaccttct atcatttctt tagccaattt aacataattg ggagcaattg
      781 tagacaaagc tggagtataa tactgagaaa taggaatatt atcaaatcca ataatagaaa
      841 tatcatctgg aataagaatt cctttctcat agcacgcacg aatcaagccc tgaacctttt
      901 catctcctga aacaaaaata atgtccggat aattttgggt agtcaagtgc tgcattgcat
      961 aagaataaac tgaatcaatt gtagataagc cataaatgac ttttaaatcc ataaagtaat
     1021 ttttatcatt cagaaaagaa cgcacacctc tttcacgatc cttattaaca tgagattctc
     1081 ctcccataag caaccacata tttttaaatt tttcttcagt tacagctttc atcatatcat
     1141 aagtagcttg aaaattatta ttagatacat agactactcc agacgtttga gattcaccga
     1201 aaacaagaaa aggcatatgg ttcttcttta aatactgaat tctgatatca tctacacttt
     1261 cataaaaaac aataacacca tctactaggc tacctgtgct tgatataatc gaattactaa
     1321 ttgtatcctc ctctccaaag tactcaacta tagcattaac accaaattct ttacacgtcc
     1381 gtaacacttt atctaacagc gtatgaaacc aaattaaagg aaaagagtcg atttttttta
     1441 cagaaatcaa tatatttata gcttcttttt tagttaaatt ttttgcatac gcatttggaa
     1501 tatacgacaa ttcctctata actttttgaa tcgcttgata agtttcttct ttaacattta
     1561 ctccaccatt aataactcgt gaaactgttt ttggagaaaa acctgataaa cgtgcaatat
     1621 cataaatagt tacctttttc ccatttatat ttttcatttc agtcctctat tacaaacatt
     1681 ctaatattac tatacaatat ttaatttttt ttaacaagag aatttagtaa attatttaag
     1741 atccacaaat tcacaaaatt aattttacaa atattcttcc ccttcaaaaa agtttaaatt
     1801 gcatttcaca cctttatttt taagaatgtt tccaacttca cgacaaataa attcatatga
     1861 gaaaaaactg ccataaaatt gtagattaac tttttcagta aaatgtgtag gatttataaa
     1921 aacatataat agcctgtcaa tgtaacattt taacatagag ttaatttttt ctttaaagat
     1981 aacatttgtt atcaactcat caggaggtaa atgaaaggca aacaccattt cacaaatatc
     2041 ataaaaagaa ataaatttgt atacttgtat caaacaatta ttatcaaaat attctatttt
     2101 acctaaatca aaattgattt tataatcttt cataaaaacc tctgagcaaa aatctactca
     2161 aaaattagat gattaaaaca tctaaaaaac aaaaggacaa aaacatctgt ccctttgttt
     2221 actaaatttc agctaatttc ttcgacataa ataacaccta caatattagc aatttcttcc
     2281 atcagtcgaa gatgttcaaa tctacctgat aattccagag taataaatga cgctattttt
     2341 ttgtccggaa catcaaagta ttcaattctg tcagaattaa catctccaaa cgctgttctt
     2401 gaatcggtca ttctgatacc attttctgca caataaacca atacacgatt ataggcttct
     2461 gtagatttaa ctactatata caattcaatc attttagaac gattttgtag atattttttt
     2521 agtggttgga acatggatat cacaccccaa acagaaatgg ctactaaaag agctccctca
     2581 taaaaaccta ctccaatagc taatccaatt cctgccgaag cccaaatgcc tgctgcagtt
     2641 gtcagacctg taattttctt tttatctgta ataagaatcg ttccagcgcc tagaaaaccc
     2701 acacctgata taacttgagc tcctaatcgt gtaggatctc ctgtcccaaa tttataagat
     2761 acgtattcat tcgtcatcat aatcaaacat gcagctagac aaacaatact ataagttcgg
     2821 atgcctgcag gctgggattt gctccctctc tctaaaccaa ttatactacc aatgactact
     2881 gataaaacaa tcctgacaac tatttcaata tttgataacc caagactagt ggctgtcatg
     2941 attatttcct tactttacgc cccggtcttt gtgtgaagta taataccgtt ccagaaataa
     3001 tcatcagaac aattgtataa acaaatacca gagcttgtgc attagatgtt gctgtttcat
     3061 cacctgcaga tcgaatcgta atacctaatg gttgagctag gggatggtaa aggaatacag
     3121 ataagtcgaa gtcagttaat aaagagttaa agtttaaagc aataacagag agaacaaccg
     3181 gtaaaataaa tggaatgata accttcatca tagtataaaa aggtgaagca cccatacttc
     3241 ttgctgcatc ttccatctca tcatcaacac taaataaaat agcacgtacc attctataag
     3301 aaaatgggat ttttacaact atatatgcaa taagtagaat taccaaacta cctaccaaaa
     3361 tctgattcaa gacaagaaat tgtggctgat taaaagtaaa taataaactt actgctaaaa
     3421 gtgtacttgg tagtaaccaa ggaagtagag caccatattc aaataagaaa tcaaaacgag
     3481 atttatgttt tctgacaaca cgagcaaata caactgcgag aattgttgct gttgtcgcag
     3541 caataataga ataaataaag ctgaccaaga atggagagaa tgccgcacta ttactaaaga
     3601 ataagcgata attttctaaa gtaaagtttg ataatgttaa gttacctgtt tgaattgcaa
     3661 ctggatctgt aaatgagtat aatactataa aaattagtgg aagcatgaaa actgtgaaca
     3721 atccatatgc tacaatgtga gcaatgatat tccaaggctt agacgcaatt ttttgttttt
     3781 taagaggcgc tttagtctta gagatagaaa tataatttcc acctttttct atcttattca
     3841 tgatagtaag caaaattgta gttgcaatac ctaaaataat tgcaagtagg gcagctaaat
     3901 cacgagaatt ccccatccct gcaaatgtaa taatcattgg atttatagtt tgaaattctt
     3961 taccaccaac aatcatgggt gctgctactg cagataaacc actaagaaaa accataatag
     4021 taagtgcaaa tagagttgga attaaggttg gtaacactac ttttcggaaa acagtaaatg
     4081 gttttgctcc catatttcga gcagcctcaa tagtgtgata gtcaacgctt cgaattgtat
     4141 ttgttaaaaa caatgtatga ttagcagttc ctgaaaatgt cataatgaat aagactgcac
     4201 catacccaat aaaccagtta gagtctaaag aagggataac attttgtaaa aattttgtaa
     4261 tcaatccata aggaccatag acaaatttat atccagtcgc taaaaccact cctccataaa
     4321 ttaaagaggt catataacct aattttaaaa ttttagcacc tttaatatca aagtactctg
     4381 tgaatagaac acaaagaata cctacgacat taactgtaat aatgagtgaa aatgctaact
     4441 taaaactgtt cataatactc tgaagtgccc tctgagattt tagaacacga tgtacagcat
     4501 caagggaaaa ttctcctcct tttacaaata cattcactac tagatcaaag tttggataaa
     4561 taataaatgt tactaagaac cagattaacc ctaaacgaat aagccaatct tttaaattta
     4621 atttatgacg catactgcac ctccttaaaa ttgcagaacg tctgatggtg tgataaataa
     4681 ttccacactt tctccgacag atctaatagc agcctgacta tcaatacttg ttacattaag
     4741 aatctgactt tcagaaactt ttattgtata gtgaattgta actccagaaa actcaacatc
     4801 aataattgtc ccttttagaa taaaatcttg ttcagtttca cgattgaatc gaactttctc
     4861 taatcgaatg taaccttttt tatcctctaa gaaaacgctt gtatttttca gtaatacttc
     4921 gtggactgtt tcatcggtca aaacattaat atctccaata aaatcacata caaattcagt
     4981 ttgagaatta tgataaatct ctactggtgt accgacctgt tcgatgtatc cattgttaaa
     5041 gactgcaatt ctatcagata aagtcaaggc ttcctcttga tcatgagtaa catataaagt
     5101 agtaatacct aactcttttt gaagtctttt caactctttt ctcaaatcta cacgtaattt
     5161 tgcgtcaagg tttgacaatg gttcatctag acaaagaatt ttaggttcaa gaaccagagc
     5221 acgagccaat gctacccttt gttgttgacc cccagataat tctgatacat tacgctgtaa
     5281 ctgttgatca gagatcttaa tttttgctgc cactgctgat actttagctt taataacatc
     5341 tggagctacc ttcttaactt ttaaaccaaa tgcaatatta tcaaaaacag tcatagttgg
     5401 aaatagcgca taagattgaa atacaatacc aattccacgc ttttcaggtt ccaaatgagt
     5461 gacatctgtt ccattaactt caatacttcc tgatgatgga tctaaaaaac ctaccaatgc
     5521 tctcaaagta gttgatttac cacatcctga aggcccaaga aatgtaaaga attccccttc
     5581 atgtatatct aaattcagat tatcaattgc aacaaaatca ccatatttaa tttgaatatt
     5641 atcaaattta atcatctcac taactccctc tattactaaa ccaaaagcct ctctttattt
     5701 cttccttaaa tttagaaata atagagagac ttggacataa aaaataactc ttatttctta
     5761 ttttacgtat tctaattcag ctttttctac ccattcatcc aaatgctttc caacagcttc
     5821 ccagtcaata ttttgtggtt tcacttgatc aacaaatttt ttcgtatctt caggtaactc
     5881 ttttagggca tctttatttg caggaataga tccaaatttt ttactatatt ctacttgaat
     5941 ttctgattga ccaaaccaat caataaattc tttagctaac gcttgttttt tactagtgct
     6001 taaaaccata gtttgttcag ttacaaatgg tacaccaatc tcaggagtca taactttgaa
     6061 ttcaacattt tgttcttttt gtccaattaa cgctccagaa ccccagatca taccgtattg
     6121 aattgggctt tctttatcta acgctttaac aactgagctt tcaccttttt gaagagtata
     6181 ggcatttgca aagtattctt ttgcaacttc ccatcctttt tctgatacgc ctaaatctcc
     6241 tttttcatca aggtaacgaa ctaagatact tgctagaatt gcccgtcctg taccaccttg
     6301 aagaccataa attgaatatt tacctttata cttactacct aattcagtcc aatctttagg
     6361 catttctttt acatcaggcg ccccaattaa aactaatggt tgaacaatca caggattata
     6421 ataattatct ttatctgata aagattgatc aattttatct aaccatttag gcttgtactg
     6481 tactagtaat ttttgatctc taattttatt tgagtcaaca gcaccaattc caaataccat
     6541 atctgcaact gcattattct tctcagcaat aacacggtct gctaattgag cgccagcgat
     6601 atcaaccatt tttatattaa aaccagcttc ttttgcttta gcagttaacc aatcaccacg
     6661 accatttgag actgagttcg aatagataac taattcttga cttttatcag ctttttcttc
     6721 agatgaagaa gcagtcgtag aatttgaacc tccagagcaa gcagcaagtg tagtaagagc
     6781 aactcccgtt gcaagtacag tagaccaaac tttcattttt ttcatgataa gttctccttt
     6841 tttattattt tatttaaatt tttcgtgata tggaacaaat tgtctcatat cttcaaatac
     6901 agtatagtca atacggttta cagtaatagt tggaatcttc tctaataaaa tttcagttaa
     6961 ttctgctctg actttagtaa actcttcttc ctcctcttcg gttagaggaa tccgaagata
     7021 cccaattgaa atatggaatt gatatctatc atgattaggg aaacaaacac ctgctttttc
     7081 tgagacataa gtacgaattt cttctaatct ctttgcagaa gcttcatctg caggttcaac
     7141 tagtatgttt tgttttccca tttcagttat acgcatatga atttcttcat ccaacaatgg
     7201 aaaaatttca agttgtttag caaagtaatc atgtatttcc tgtaaaggtg tatctagagg
     7261 aagattactg ctccaaaact ccgtttcacg attttcatgg cacaacaatt caattacagt
     7321 catgtgaata gaattccttg gagttaaagt aaacttatcg ataaatggta attctctata
     7381 acgtgattga ataatatcaa caacttccat caaatcttgt ttagtataaa gatttgctac
     7441 aactgtattc ccagagaaat gattaaattc cccattctct ttgaacttaa tttttgttaa
     7501 gtttttcata taaagacctt cttttttata tttgacaccg gtgtcatcat gtttacatta
     7561 tacaatgaaa ccgtttcctt tgtcaagcac ttgcaaacat ttttttaaat ttatctttca
     7621 ttaaatatgc atttcattaa aaggttaagt aattttttca aaatacagca ctcatataga
     7681 cagtatcatc aattatagcc attgggtttt tttgattgcc aattccatgt atctctacac
     7741 atgtcttcaa tcgattttgt tgtcctccaa tttaattcct tatatgcttt gtctgcattt
     7801 gcataacaag ttgcaacgtc tcctgaacgt cttggaacta ttttataagg aatagggatc
     7861 ttattaacac tttcaaatgt atttacaagt tgtaatacac tagtgccttc tcccgagcct
     7921 aggttataga tataaacatc tgttttttca gatacttttt ctaaagcttt tatatgtcct
     7981 attgctaaat ctactacatg gatataatca cgcacaccag taccatcaag cgtatcataa
     8041 tcatttccga acacacttag ctctgatagc ttacctaccg ctacttgtgc aatataaggc
     8101 atcaagttgt taggaattcc tgagggatct tccccaatca aaccagactc atgagcacca
     8161 attggattga aataacgaag caacgcaata ctccattctg aatctgccac atgaacatct
     8221 tttaaaattt gctcaagcat cactttcgta tacccataag gatttgtcgc acttgtttgc
     8281 atcgtctcaa ttagaggtga ctgattgtta attccatata cagtcgcact tgaagaaaag
     8341 acaatctttt taacattaaa ttctgacatc acttcaacaa gtgccaatgt actcataata
     8401 ttatttttgt agtacatcac aggcttttgc acggattctc cgacagcttt ataacctgca
     8461 aaatgaattg cagcatcaat cgattcttgt tcaaatacct ttctcaatgc ttgtttatca
     8521 caaacatcta attcgtaaaa cacgggacgt attcctgtaa ttgcttcaat acggtctagc
     8581 accaatttgc tagagttcga aaggttgtcg acaatgataa cttcctttcc taaatttagt
     8641 aattctacta cggtatggct accaatataa ccagctccgc ctgttaccaa tattgccatc
     8701 tgggtttcct cctaattaat tccaaccgac ttaacaaatc tcataaacgc ttcatgccca
     8761 gacggtgtat tcttataaac tcctgcatct tccagaactc tcgcaaacac ttgtcctgct
     8821 tcgtgttgaa ctacgctatt aacctcttct ttattaatgc gaggatattt ttctttcaat
     8881 tggtcggccc attctaaatg ataatccgca attgcattat cctctcctaa aagatatttt
     8941 ccaacttctt ctaactctgg tttcaaacga ggtggtaata tcgcaagtcc catcacttcg
     9001 attaacccga tattttcctt tttaatatgt tgtacatctt gatgaggatg gaaaacacca
     9061 tctgggtatt gttcagtagt atgattatct cttagaacaa tatctaattc gtatctcccg
     9121 tccactttac gagcaatagg agtcaccgta tggtgtggga catcttcagt catagcaatg
     9181 atgtctactt ctaaatctga atattctctc cacttattta gaattttagt agctaaatct
     9241 aacaagcgat ttttattttc actttgtaac ctaattactg acattggcca ttttacaata
     9301 ccagcattaa catcctcaaa gtctttaaaa caaaattcac tctcaaattt tgctttttcc
     9361 attgggaaaa tatgtttccc tccctggtag tggttatgac taagaatgga gcctcctgag
     9421 ataggaagat cagaatttga accagcaaaa tatcctggca aaatatcaac aatctccaat
     9481 aattgttcaa atgttttaga ggtaatagcc attggtacat gttgactatt caaaaatatc
     9541 gcatgctcat taaagtatga gtagggagaa tactggaatc cccatacttc gtcaccaagt
     9601 ttcaaccgaa taattctatg attcgaacgt gctggataat ttattcgccc ctgatatcct
     9661 tcattttcca tacatagtaa acatttggga taattagttg cttttactaa tttttcagca
     9721 gcaattgttt ttggatcttt ttcgggtttt gacaaattta tcgtaatctc tagctctccg
     9781 tatttagttg atgctcgaaa ctcaatattc ttagcaatag cagaagtttt aatataatca
     9841 ctatctttac ttaacttata aaactcttca actgcttctt gaggtgatat atcatatgaa
     9901 ctccaaaaaa tatcatttaa tcgactaggt aaaggaacta tgaaattcat taactctgct
     9961 cctaaacatt ccttttcctc gattaaatct ttaattttac cgttttttaa ggcgatttcc
    10021 actaagtaat cttttatttg tttcaggtca ttttcatcgg aaatgcgatc aattccctcc
    10081 tcacctatta acgctagtac tctatttttc acatatattt tgtcaatttc attatacatt
    10141 ccgtattcaa ttactctatc aacaaaatta tcaataattg ttttcatata tttttctttc
    10201 taatttatgt tcccatattt tctatacatt atccatttat aaattgcttg cgtagtatga
    10261 gcaattttat caaggtgatg aataatatct aaagcactaa ttacttcaga aacgttccca
    10321 tcatcttcaa atatgtaatt cattattttc ttttccatat ttatactaag ctcttctatc
    10381 tcattctgtt tttgtataac aaccatatct aaacatccag attgttcctc tctataacaa
    10441 gatatagccc tattcatatg cagtccgata acttcatgaa gtatttttat ttctgaaata
    10501 attttcttca aaatttcatt attttgaata atctgtagat tttttaaaat ttcaacaatt
    10561 ctatccccaa tacgttcaat gtcagttgat atttttatta cactaataat tcttcttaag
    10621 tcatatgaaa cgggatgttg taaacaaatt aactcatatc cttttttatc aacatttaga
    10681 actgactcat ttatgattaa atcttcttta atcaattcta ttcgttcttc atttgataaa
    10741 tattcaaata acttctcata tttatcaagc acagataccc aaatggtctc taaattattt
    10801 gataattcta taatttcatt ttctaaatat aaccttaaca tttaggtacc tcttcttaac
    10861 aaagttcaat agtaacaatt aatattttaa acaatatatc aaacatcaat aactacaata
    10921 cttgcatcat ctttctttcc atagattgga tcaatagcag aagaattaaa tctcatctta
    10981 attaactctt caaaagtttt attttgatta ttttgataga attcataaaa gccatcgctc
    11041 attaaaacaa tttgttcact agtaacatct atttgattaa taatagcatg gtctaaaaat
    11101 ctctcatcca acgaacctat ccagtaccca ctcggttgat tagataattt tctgattttt
    11161 tgtaaaataa tttttttatt taaaacacta tttgtaccaa ttggatcttt tatctcattt
    11221 ttcccttttt caaataagtt atctactcta tgatccgtta tttccatttc gtttactaac
    11281 atgacgcagt cacctagcat catatactcc aacttttttt ctgaaagttt agcaaatata
    11341 aaagcaaatg ttggtgagcg ataatatagt atattgaaac tagaatagta cacctctact
    11401 tctaaaacat tgttagaaat cgatttgact gtcctgattg atttgtccta ttattatttc
    11461 attttattat actctgttaa ttcatatgag tttaaaccga tttcatcttt aacctcgagt
    11521 aaagcagttt caaatatttg tttaagagtt tttgattctt tacaattaac cgacaaactt
    11581 tctgataaaa tatgtacaac ttctgagact gaataaccta tctcctcttt agaattatat
    11641 aaatctgtag ctccaccaat aatccaaaaa tactgatttt gtgaacctac aatatcctca
    11701 ttttctacgg aacttccttg tatcgaacaa attttattta tctttaccat aatacttcaa
    11761 cccttttagt gtcaaaagta aaccaattcc tgtcactgtt aagaatagtt ccataatctt
    11821 gttcgaacca gtctttggta atttttgttt tacatctact atttctttag atttattaat
    11881 atgattttca gtttctctgc catctccaac tattttatag tttacttctt ctgtcttatt
    11941 atcttgttta ttgtcgatct tgtcattcat ttgtctatta tctttacttg agttaaactc
    12001 tccgttcttc tggttactat caattacatt atttgaatta gattgttttt cctctttgtt
    12061 tttttctttt tcgtttttat cacttaaatt atttgttaca attttgtaaa gcccattctc
    12121 cgttacaata ttgaaattac catcgctatc acgtataaca ggttctttcc cgtttgcatt
    12181 agatttgatg aatgatatat acttaccgga taaattataa aattggttat ttaaaacggt
    12241 tattttaccc tttgaatcct caataacaat tccttcttta ccaccgaata tactattctc
    12301 tgacactact aaattatcac taactcgtaa taattcaata tttggttgtt ctgtttcctt
    12361 agaattatta ttaattacat tttttgttac agtaatatct gatacttttc ctaaatattc
    12421 tgcactatct tttgcaactc gtatcgcttt tgttttagga tcggcaatat taaatatatt
    12481 ttcggcgata accacctgtt tatttaaatc taataggtct ttagtgtttt tatagctata
    12541 agcatttact aaagctgctc cgctttctcg ataatgtaca ctctctcctt taactttctt
    12601 atcaaagcga tttcctttga ttaatacatc agtgaatcct gtaaaacgta cacctgcata
    12661 catcatgtta tcaaaatgat tatttaaaat tttaatatta gaggggttct gtgtcgacaa
    12721 tgtttgatag tgtgtgccaa ttgctgttac taattcccca gatttatcac ttttgccaaa
    12781 ataggaattt tgaatagtca cattttcaga ttttttccca tcatcattca aggcataagg
    12841 aaaacctttt ctagttaatg gttcaatctg aatgctctcc ttacttatga tttgcccatc
    12901 cttcatcgtt ttgggtaagg cttgcccaag aaaacgagaa ttatcaacta atacattttt
    12961 cgaacctgca atttgaatag catgcccttg ataactatcc ttgaatgtta cattttttat
    13021 agttacgtta tttgaattcc caatagcaaa tgcacctgaa gaatttataa gtggtagatt
    13081 ttttgcttta gttccttctt cattcaaagc accgttcata tcaatcgtac caccagaata
    13141 actaatatct tctattgggc cccattctac ttgcgcacca tcatccgtaa ataaacctgt
    13201 cataaaaaca atggaagggt gattcttaat atttatacca tttagaattg tagctttctc
    13261 attcaattct aagtgtgtat gactttttaa aaaaacaatt tcttttacta aataagttcc
    13321 ttcaggaaaa tatacatttc ctccacctag cccttgagct gcagcatcta ttgcatcttg
    13381 aattgcttgt cgatcatcat gaatcccatc acctactgca ccataatctt ttacacttaa
    13441 agcagtatct tcaaattctt ttttgataat tctgtcactt ataacatctt ccttataact
    13501 cttattttca ttagtagatg ttactgtaga ttcaactgtt ctgttgtcta attgtgaatt
    13561 ggaacgaata gtaccattag agctgatact attaacactt aacgaattat ctaaaccatt
    13621 agtggttgaa ttagaggcaa tcatttcagc agaacgattc tgattaacag ttggtgaatt
    13681 atctaaatca ttagtaattg cattagaaga ctcttttgta gccatcggtg aagaactagt
    13741 aacaacttca tccgataaaa taccttggct tcccaaaatt gaaattgcca atagtaccga
    13801 gcaagtcccc acctttaatt tacgaatact gaatacctca ttaggaacaa aatatttcat
    13861 cagaacctcc aattttttta ctatttttaa caattgatta ttacttttct taaaatagat
    13921 agaatgacac cgctgtcatt atctttatca tatattttcc aaattgaatt gtcaaggaat
    13981 aacacaagaa aatattaaaa tttatactct tcggaaatct cttcaaacta ggtcagtgcc
    14041 gccttgccct aagtctaggt tactgacttg gtcagttcta tagtagactg aaactataat
    14101 agtacacctc tacttctaaa acattgttag aaatcgattt aactgtcccg atcaatttgt
    14161 cctattcttg tttcatttta ctatatctac aacctcaaag cagggttttg agctatctat
    14221 ggccaacttc ctagtttgct ctttagcctt ccattcaaaa gtgatgctat ctttcttagc
    14281 ttgaatataa aatatcccat ttactatatt taaatttgtt gatatgttgt aaattataaa
    14341 tgaaagaaca aatatcttgt catacaagca ttcgccaggg atatttacat gaatatcgct
    14401 catatggcgg attgaaacta aaacactaca atatatttct taaatagacc ttctataaaa
    14461 gctagattta acctcgataa aaatagatgc agtgcttcta aaatactgga atagggcata
    14521 taggtaatcg tagacaagtg ttttatagat tgtctcgcgg gagatctaat gatgtatagc
    14581 aagcatcact aaacatatgg gatatttgcg ttgttataat atcaacatgc tataaattct
    14641 atcatgcaaa agaatgacta gaacatgaaa gacaggcaca aaactttgaa caatattctg
    14701 aaaacaaaaa aaagacgcta gtttccacaa tctcactcat ggaaactcac gtctttcaaa
    14761 aaatagggtg aattgaaaac tagaacaaga cactagattt tctaaatgat gtctagtaag
    14821 gattctaaat gctataccaa attgctcaca tcttatttca aaccacggga ctcccacaag
    14881 aaaggaaaat cctataaaaa ttctctctct gaggagaaag aggcctcaac tacaggtgta
    14941 aactattatc aattgaaaac aacagttttt acactcaatt agacttttta gggacaggat
    15001 cactttcgct tgactgtagt ttgtcatttt tttgataaag ttagcacttt ttcagaaact
    15061 gttttgaaag tctcaatgat atagtctact tcttcatcgc ttaatttagt atgaagaggg
    15121 agggtaattt cattctcaaa gaaggcatag gccttaggat agttcgtcat atcaaatcca
    15181 agattcttat aggctgtcaa gagaggaagc ggtttgtagt gaacattact tgcaattcct
    15241 gctttagcca attcttggat gatgaggttg cgttcttcta ggcttgctcc ttctacacgg
    15301 gtgatgtaga ggtggcgtga agattcgaca gtttcagtct tgtgtgccaa aggatggatg
    15361 cgagaacctg caaaaccact atcatagcgg tccacaatgt ccttacggcg ttgcaacaaa
    15421 cttggatagc ggtccaattg taccaaacca agtgaagcca tgatatcggt catgttgcac
    15481 ttataggctg gtgtaacgat atcgtattcc catgacccca attgcatctt ggcaagagca
    15541 tccttagttt gcccgtgaag ggaaaggatt tggaattcct tgtacatctc ttcgtcatca
    15601 atcactggat tggctttcca agtcgcactt ccaccttctg ccgttgtaaa gttcttaacg
    15661 gcatggaatg agaaggaagt aaagtcagcg atagaaccag aaggttgtcc tttataagta
    15721 gatcccaaag cgtgggcact atcagagaca atgacaatac ggttaaaggc cttttgccac
    15781 ttgcttgaag cggtaaagaa gtcacgtttt ttctccacga cttggaacaa acggtcataa
    15841 tcgcaaacaa tccctgcgag ctctactggg ataatcacct tagttttctc agtgatagct
    15901 tgctcaagca ggtcatagtc catctcaaac gtatctgctt ggatatccac catgacaggg
    15961 gttgctccca cgtgcgtaat gacactacat gaagccgtat aggtcatggc tggaacgatg
    16021 acttcatcac caggtcccac ttccaaaacg cgtaaaatca actccagagc ggctgtcgca
    16081 gagttgagac aaacagtctt aggtgtctgt gtgtaaagag acaagcggcg ctccagttct
    16141 tttgttttag gacctgttgt gatccaacca gaacgcaggg tatccgctac ttcagcaatt
    16201 tctgcttctg tgatatcagg cggtgaaaat ggaatattgt aatttggcat tgatttgctc
    16261 cttttatctg tactcatttt cttatgacta ctttatttta gtacctcaaa cacggtttga
    16321 aacatgattt tgatgtcccc aaagaaacta aactctcgga gataggcgag gttatagcgc
    16381 atcttttcag gaagaacatg ctccacatag gcctgatcaa ctgacagacc tttctccgtc
    16441 atttgactga tgatggtgtc ctcatccttg tagttgatgc tggctggaga ggtaattcct
    16501 gcttgcaaga gcaaggttgc catcatttca gggctatact gctctgtata acgtggcact
    16561 tcaggtcgtg taccgacaaa ggacatctca cctttaagga cattgaccaa ctgaggcagt
    16621 tcgtccaaac ggacacgtcg gatgaaattt ccaaccttgg taatacggct atcgttagca
    16681 gaagtcacca gacttccttt tttatccgca tccgtcacca tggtacggaa cttccaaatc
    16741 ttgaaccgac ggttgtactg ggtcacacgc tcttgcttgt aaatcactgg ccccttgcta
    16801 tccaacttga tccaaatgct caagatgaga aagatgggag aagttagaac cagtaagacc
    16861 aaggccaaaa cccagtccaa gcaacgcttg aaaatcagcg aacccttcct tttagagaca
    16921 agctggtagt aagactcaac ctcgcttgat ttcatttcca caggcaagtc ttcccatttc
    16981 agcatgctgt tctcctttgt ttttattata ctatttgtca acatttttca ttataccaca
    17041 aaatggaaaa ggcggtgaaa gaaatctgta atttgaagga tttccttcta gtactcaatg
    17101 aaaatcaaag tacagctttg agattgcaga taaagctgac atggtttaaa gagattttca
    17161 aagagtatag gccatggccc ctgcctgcaa actatacatc ttgtgatagg ttcctcccag
    17221 agtcaagagt tcctcatggg ttccactctc gataatgcgt cccttatcca agacatagat
    17281 gcagttggcg tcttgaatag tagaaaggcg gtgagcgata gcaatagttg ttcggccctg
    17341 tctcatcttc gccagagaag cttgaaccaa gctttctgtt tcagagtcaa tattggctgt
    17401 cgcttcatcc aaaatcagga ttttaggctg gctggcgact gttctagcaa aggcaagaag
    17461 ctggcgctgc ccagtagaga agctcgaacc acgctcggaa acaggggagt cgtacccctg
    17521 aggaagttct tgaataaagg aatctgcatc cacaaaggct gccgcagcct gaacctgatc
    17581 atcactgatt tcttggtaca tggcgatatt ggacttaata gttccatgat agaggaaggg
    17641 ttcctgcaag accaaaccga tgttttttct cagctcttct tgactgaaat ccctgatatc
    17701 cacatcatcc aagagaactc tccctgactg gaattcataa aagcgcatga ggacattgat
    17761 aatagacgat ttccctgaac ctgtatgacc tacaaaggca atggtttcac ccttattaac
    17821 agagaaagaa atgtcatcca gaatcggatg tttaccatca tatgagaaac acacatgttc
    17881 aaaacggata ttgccttctt ggactttggc ttgcccattt tcttgaagag gttcataggt
    17941 cctctcgtct atcagggcaa agacacgacc tgcagaaacc atagccgttt gcagagttga
    18001 aaagttttgc gtcacctcaa tcaaggggtc aaaaaggcgg ttgatgtact ggataaaggc
    18061 atacatggtc ccgaccgtta tcccgataga aaaaccacgg tagccaaagt aggccatcaa
    18121 gactgcatag cctagaagtt tcagcaaact catggcaggt ctcaaaaaga gggcatccaa
    18181 ggctacagaa cggttggcgt agaccaagtg ttcttggttg atttcatcaa attctgcctg
    18241 caggcgcttc tcttgattaa aggcctgaat aatcctgatt ccctcgatat tctctgccag
    18301 cttactattg atatctgaca agagacttct ggttttctcg atgattttca ctgacttttt
    18361 tcgatagaga ttgaccaaaa ggaaaatcaa aggaagaaag agcaagacta aagccgtcaa
    18421 acgaaaatcc agcaccaaca tggtataaag ggttgtcaga aagataaaaa ctgctgagat
    18481 aaagctggat aaaatcccag aaaacatatc actaatcgtc tcggtatcgt tggtcaaacg
    18541 agaaacgata gaacctgctg gcgtcttgtc aaagtaagac atgcccagtt tctccatatt
    18601 agcaaaggca tctcgacgaa tatccctaac aatactataa gacacgcgcg caaagagaag
    18661 attgcccaca tactgaacta cagtttgtag gatgtagaga ccatagtaga ccagcaaaac
    18721 ggtaacggct agttggttaa gattgctgag atactggtcg ataaagtggg aagccacgag
    18781 gggtatgaca cttttaatga ccgtcgtcgc taggagaaaa ctgagtgcca aaaaggtcag
    18841 gagtccataa ggcttgagat aagacatcaa gcgcttcaat acagcccatt gttcttgttt
    18901 attctgcatc ttcttctcct ttcatttcca actgctgaga ctggtaggtt tgggcatacc
    18961 agccatccat cgctagcaag tcttcgtgcg tgcctcgttc gataatttga ccattttgta
    19021 gaactaaaat aaaatctgca tggacaacag cactgaggcg atgggcagtg ataatggttg
    19081 tcttgtcctt tcgcgtctcc ttgaggttgt cgataatcgc atactctgtc ttggcatcta
    19141 cggcggataa ggaatcatcc aaaatcaaga tatcagggtc taaaatcata gcccgactca
    19201 tagccaaccg ttgcttttga ccaccagaaa gactgactcc tttttcacca atcagcgtat
    19261 caaatccttg aggcatgtct acaatatctt ggtaaacccg ggctagctta gtagcttcct
    19321 cgaccgctga aaggggcaag ttaggattgc caaagcggat attgtctagg attgaagtcg
    19381 caaaaagaaa ctggtcctga ggaacatagc ccatgagact gcgaaggtct gtcagacgat
    19441 agtcccgaat atcgtgaccg tttagataaa tggcaccctt atccacatcg tattcacgca
    19501 agaggagctt gattaaggac gttttcccag agcctgtctg cccaaccaag cccagtgttt
    19561 gcccttttgc caaactaaag tgaatatccg tcagtgtttc ctcattttca aaagcaaagc
    19621 tgtcaatggc atactccaaa cgcccatttt caataccatc cagaggaaac tccgggtctt
    19681 gtacaggaga ttcctgagac aaaagatttt caatccgctg gtaagaaacc ttccctcgct
    19741 gagtagtatt aaagaggaag ccgatggcca taagaggcca gaccagcata tccaaatagc
    19801 tgataaaggt gactagattc ccaactgtaa tctgcccttc ctgaaccacc aaggagccaa
    19861 ccaaaagcgt taaaacatag gacgaaccaa caaacaagag aaccataggg tcaaagagac
    19921 tatcatattt catggtttgc aggttctttt ggaaggttaa ttcattgact gcctgaaaag
    19981 acttcaactc gtctgcctga taaccgaaag acttggtcac tttgatacct gatacggact
    20041 cctgtacctt gttattgagt tcagaaaaag cagcttggga ttcgccaaag gccttatgag
    20101 tctttctccc taggcgacta gtcgcatagg ccatgaaagg tagggggaga atggcaacaa
    20161 gagtcatctg ccatgagatg ctaaagagca tggtcaacaa agtcaccaga gccgtgatag
    20221 aggcatccac cgcagacatg acaccgccac ctgctaaacg agtcaaggca tttatatcat
    20281 tggttgcgtg tgccatcaga tcacccgtcc gataggtttg ataaaaggct gacgacattt
    20341 ttgtgaaatg cttaaacaag cgagaccgca tgatctgtcc caagcaataa gaggtcccaa
    20401 ggatatacat acgccacaca tagcgcaaat agtacatacc aaaggctgca agtagcaagt
    20461 aaaataggct aagaaggagg tcctgctggg ttaattgccc cgatgtgatg gcatcaataa
    20521 cccgccccat aaccatagga ggaatgagat tgaggacgga aaccaagacc agggccacaa
    20581 ttccgactag ataacggcgt ttttctaact tgaaaaacca ccaaagtttt tgaataatgg
    20641 acataaaatc cctttctgat tgcaaatgga aacctgaggc caatactcaa tgaaaatcaa
    20701 agagcaaact aggaaactag ccgcaggctg ctcaaagcac tgctttgagg ttgtagatag
    20761 aactgacgaa gtcagtaacc tacatacggc aaggcgacgt tgacgcggtt tgaagagatt
    20821 ttcgaagagt acaagacccc aggtttttct ttttcataag ttacgactgt aacagcaccc
    20881 ttgtcatatt cagcaataaa gatattggct acattgtcat gcccttgttt actgaggtta
    20941 tcaagcaacc actcttcgct acgaccaatc gattccaaga catctacttg aatcacaccg
    21001 tcagtcacaa ctggatactt aggattttca tctcccattt gcacaacgat gagttgccca
    21061 ttttgctctt gcacagctcg tttgacttgc ttcatctgga aaatcccttg gctacgaagt
    21121 ttgagggcta catccgatgc agacaaacca actgaacgac aggtttatgg gtcaatattc
    21181 ccatttttga taaggagagt tggcttacca tcaatcaaac gtttcacaaa gtgaacattg
    21241 ttgttgagct atttgagagt caagaccagt ttgattaaaa tttcgatata attaagtgtc
    21301 atttgttcac atccaccagt tcaattgcgt ctgtcttata caattcgact ttctctaaca
    21361 ggtacttgtc tggctcactt ccgtttaggg cacgataata gcaatctccc accttgataa
    21421 gtgcgccatc tgtgtttgtg gaagtattaa catagacttc tgacttgtct actcccaaat
    21481 ctttggaaac aacctcgatg aaatgaagtg aagtttgaaa tttattgtta gaaacttgat
    21541 tgttttgata agtcgtgatg ctgaccaaaa gcagagctaa ataaggtcag agctgaaatc
    21601 atgaccaact cacgaaattc ggttcctttt ttatcattgt atgccttgaa agcaaaaaaa
    21661 actgtcacag ctaatagaac aatcccaaag ccaaccatga taccattttg ctgaccgatt
    21721 tggctgagta catagtcata agagtagaat ttcatgtatt ttcactccct ttcataaaat
    21781 cattcttaat tataaacgaa agagagtaat ttttcaaacc atacgttggg gaaatatatc
    21841 tttttttggt ataataaaat ctataatctg aatgaaaaag gtaactttat gaaacttatc
    21901 tcatggaata ttgattccct aaacgctgcc ctaactagtg actcagctcg tgccaaattg
    21961 tcccaagaag tcctacaaac cttggtcgct gaaaatgctg atatcattgc tatccaagaa
    22021 accaagcttt ctgccaaagg acctacaaag aaacacgtgg aaattttaga agaactcttc
    22081 ccaggctacg aaaacacgtg gcgttcttcc caagagcctg cccgtaaagg ctatgctgga
    22141 accatgttcc tttataagaa agaacttaca cctactatca gcttcccaga aatcggtgcc
    22201 ccttctacca tggacttgga aggtcgtatc atcactctag aatttgatgc atttttcgta
    22261 acccaagttt acactccaaa cgctggtgac ggtctcaaac gcttggaaga acgccaagtc
    22321 tgggatacca aatatgctga gtatttggct gaactagaca aagaaaaacc agtccttgcg
    22381 accggtgact acaacgtagc ccacaatgaa atcgaccttg caaatcctgc tagcaaccgc
    22441 cgttcacctg gatttactga cgaggaacgt gctggattta ccaacctttt ggcaactgga
    22501 tttacagaca ccttccgcca tgttcatggc gatgttcctg aacgctacac ttggtgggca
    22561 caacgcagca aaacttctaa aatcaacaat acaggctgga gaatcgacta ctggctcaca
    22621 agtaaccgta tcgctgacaa ggtcactaaa tctgatatga ttgactcagg tgctcgccaa
    22681 gaccatacac cgattgtctt ggaaattgac ttgtaaggat tttctatatg gactacaatg
    22741 cggtcattcc cgagtttctt gtatcgaata tcgaacagtc ccggtccttc tactgtggtt
    22801 tactgggttt taggattgaa taccaacgtc cagaagaaaa ctttctcttg aagagtgtca
    22861 actaatgtta gaggagggaa caaaggatca actagctgaa ctgacctacc cctttggtcg
    22921 tggagtcaat ctctcctttg gcataaagga tgtttcaaaa ctctatcaaa aagtgatgga
    22981 ggcaaactat cctatttatc gtcccttgac caaaagaaag tttcgtgtca gtgatcccta
    23041 tatctatcct cataaatttg cagttttgga tccagatggc tattttttaa gatttagcga
    23101 gtagaataat aaaaattggg aaaagtcttt aaagttagaa aaaggcatat tgtcaggtat
    23161 ctaaaaactt gatagtatgc cttttatgat ggaaaaatct tctaatactc ttcgaaaatc
    23221 tcttcaagcc acgtcaacgt cgccttaccg taggtatatg atactgactc tgtcaattct
    23281 atccacaacc tcaaaacagt gttttgaggt tgtggatagc ttcctagttt gctctttgat
    23341 tttcatttag tataaatgtt agtttacaat ggagtttttg ttcaccctct cttctgtttg
    23401 ttcgtttttt aaaccaaaac actaaaaaaa tagttgattt ctaaagttat ttagtgtaaa
    23461 ataaaaacat tggctcaagc ctatttaaaa ttgaatatcg ttttacttgt ttatgtcgtg
    23521 aattggcacg acgtttctac aaggtgccgg aacacctaac aataagtaag tcagcagtga
    23581 gatatggccg tttttgccat gtctatttag gggacttact tagagattaa gtaggtcttt
    23641 tttgaatatt tttaatattt cccttaatta gtgcggacgg gaggtgaaat tatcctgtga
    23701 ataatttcag gaatccaggg aatttcatcc ctaatttttg agcccctagc tctttcattt
    23761 ctaggcttag gctaaaatag ttccccgaac tattttactc tcaaaaatcc cgtcatagac
    23821 aataacaaga ttattgtcta tgataccact acaacgggaa atatttgata agactcgctt
    23881 cgctcgtaat agttttatca aatatatagt attagcaaaa gggatcacta tagaaacatt
    23941 taaacaacaa gtaaattaac ttttagactt taggaggtct tatgaaatta ttagaagagc
    24001 gcatcctcaa ggatgggcat atcttgggtg ataacatcct caaggtagat tcctttttaa
    24061 cccaccaagt tgactttagc ttgatgcgag agattggtaa ggtttttgcg gaaaaatttg
    24121 ctgctactgg cattaccaag gtcgtaacca ttgaagcgtc gggtattgcc ccagccgttt
    24181 ttacagctga agccttaaac gttcccatga ttttcgccaa aaaagctaag aacatcacca
    24241 tgaacgaagg catcttaact gctcaagtct actcctttac caagcaggtg accagcactg
    24301 tttctatcgc tggaaaattc ctctcaccag aggacaaggt tttgattatc gacgatttcc
    24361 ttgctaatgg ccaagctgct aaaggcttga ttcaaatcat cgaacaggcc ggtgccacag
    24421 tccaagctat cggtatcgtg attgagaaat ccttccaaga tggtcgtgat ttgcttgaaa
    24481 aagcaggcta ccctgtccta tcacttgctc gcttggatcg ttttgaaaat ggtcaggtcg
    24541 tatttaagga ggcagatctc taatgcaaac tcaagaaaaa cactcgcaag cagccgttct
    24601 gggcttgcag cacttactag ccatgtactc aggatctatc ctggttccca tcatgattgc
    24661 gacagccctt ggctattcag ctgagcagtt gacctacctg atttctacag atatcttcat
    24721 gtgtggggtg gcaaccttcc tccaactcca actcaacaaa tactttggga ttggactccc
    24781 agtcgttctt ggagttgcat tccagtcggt cgctcccttg attatgattg ggcaaagcca
    24841 tggtagtggc gctatgtttg gtgcccttat cgcatctggg atttacgtgg ttcttgtttc
    24901 aggcattttc tcaaaagtag ccaatctctt cccatctatc gtaacaggat ctgttattac
    24961 cacgattggt ttaaccttga tccctgtcgc tattggaaat atgggaaata acgttccaga
    25021 gccaactggt caaagtctct tgcttgcagc tattactgtt ctgattatcc tcttgatcaa
    25081 catctttacc aaaggattta tcaagtctat ctctattttg attggtctgg ttgttggaac
    25141 tgccattgct gctactatgg gcttggtgga cttctctcct gttgcggtag ctccacttgt
    25201 ccatgtccca attccactct actttgggat gccaaccttt gaaatctcat ctattgtcat
    25261 gatgtgtatc atcgcaacgg tgtctatggt tgagtcaact ggtgtttatc tagccttgtc
    25321 tgatatcaca aaagatccaa tcgacagcac gcgccttcgc aacggttacc gcgcagaagg
    25381 tttggccgta cttctcggag gaatctttaa caccttccct tacaccggat tttcacaaaa
    25441 cgttggtttg gttaaattgt caggcatcaa aaaacgcctg ccaatctact acgcagctgg
    25501 tttcctggtt ctccttggac tgcttcctaa gtttggtgcc cttgcccaaa tcattccaag
    25561 ctccgtcctc ggcggtgcca tgctggtgat gtttggtttt gtatctattc aagggatgca
    25621 aatcctcgcc cgagttgact ttgctaacaa tgaacacaac ttccttatcg cagctgtttc
    25681 aatcgctgca ggtgtcggtc tcaacaacag taatctcttt gtcagcatgc cgacagcctt
    25741 ccaaatgttc ttctcaaacg gaatcgtcgt agccagccta ctcgctattg tcctcaatgc
    25801 cgtattaaat cataaaaaga aataagaaaa agaggtgcga gcctcttttt tgtgtgacaa
    25861 aataaaaaca tctacctatt catatatgct agtgtcatag tcctgaaaat catctgcatc
    25921 aagaacaatt tcacaactct tatacttttc tcttacaagt cgttcggctt catctggatt
    25981 ttcagcctct atacttacta aacgtgataa agtttctgta atttctactg tatacttttt
    26041 cagttttaga ttgctttcca ataattcacc tattaacaat aatctatgat aggagctatg
    26101 tagccactct ggcaatttat ctcctacttc ccacccatat ttttccttca tcaaagctag
    26161 ttgtttttcg tcaccaaatt tcatattttc agataaccat ctcacacaat cgggatctga
    26221 taaggtacag acgattaaac ttaggttatc gtaacggctt tcagtttcat aatcaggcca
    26281 gatgactttt aagaaatcat acacctcttc tgatgcaaca agttgtttcg ttttcataat
    26341 ttccgatact ttcctctacc tttaaattct attatttctt tgtctcttaa tatttgaagc
    26401 tgttgcctaa tcttttcttt gatatgattg ttcttaacaa agatattttt taggtcactt
    26461 tcaaaacgat acatatcttc aagggtaaat tctgaaccct ctatcttatc tatacaattt
    26521 agaatttcta ttgtccaacc tcttgatgac agagagctct tccttaaaaa taaaccttgc
    26581 ttaaattctt ttgtaacttt ttctggatct ctaacttgtc catcttgcac aagaaatatc
    26641 cttcctttag aaggtacttg tgataaatca atattacaac caatccaacc tgctcgtcta
    26701 gcagttggtg caagtggttt tctttgaata atcgatttcg gtgtaacaaa ttgcttcgga
    26761 aggacaagaa agttatttac ctcaaaattt tttgtgtaag ttaaaaaaaa gaaattagga
    26821 ttattatctg cctgcacacg cttcatcatc gttgcataag caccatcatt gattgttgat
    26881 gaaaaatttc cttttttgct ctttagttca aactcctcac tacaatgatt acagtaaaaa
    26941 tctgctacag gccgattatt ttcaaaatga tttaagggat tgttcccaca attagggcaa
    27001 taagactgac gataaaccca atcctccgtt aagatacgtg ccttttggct attactttta
    27061 taggtttcta ctaattctaa attaaagtgt aattccattt aaaaattcct cactaatatg
    27121 agaactcact cgatataagt aattaaaatc tactataact agtataccac atatatgtac
    27181 ctattacatt tgtatttcat cactctcttt tatatttcct tctaacatat ctcataaatt
    27241 aaaaatgagt gagataaatt tatcccactc acctttcttt atatccttac ctgtctgtct
    27301 tctgaccgct ggcctgtgac aaacatggta aaggttgctt tacagacatt tttgccctct
    27361 tgattggtga tatccacatc catcacacag gttgtacggc cttgatggac acattctcct
    27421 ttaatggtca atacatcgtc gagttttcct gccttaaggt agttgataga ggattggagt
    27481 gtcaccccat caagtcccag cgagataacc accaaaccac tgatttggtc acatagcgtg
    27541 aatagatagc caccatgggc attgccatag taattgagcg acgagttcac tactttggtc
    27601 gtcaccacaa cgtgaccatc tctcattttt tcaatttcgt aattttcaaa ggcagatata
    27661 gcgtcaaaat gaaaatcttt catcgtttcc tcctgatatt tcaaacgaca ctatgatact
    27721 actttttata ggactgtgca aggagaaagc tcctagtcta gtgaaattcc gacatgttcc
    27781 acaaagcgca taaaggctgt ctgcccttgt tctgtctgct tgtagactcc tgcatcctca
    27841 agtacacgca caaagatagc acccacagag tccttgacga ttgcaagggc tttttcttta
    27901 tccgttaggt ctggatgttg ggatttgagt tggtctgccc actcctgatg ataatcggca
    27961 actgtaacag cttctcctac aagatagcta gcgacttgct ccacttcttc tttcagacgt
    28021 ggtggcaaga ttgccaagcc catgacctca atcaagccga tattttcctt cttgatatgt
    28081 tggacatcct tgtggggatg atagatgcca tcaggatgct ctgctgaagt ctgattgtct
    28141 cgcaagacca agtccaactc aaactgtcca tcacgcttac gcgctattgg tgtgatggtg
    28201 tgatgcggtg tcccgtctgt ctctgccaaa atctgcacac tagaatctga atactgacgc
    28261 cattcctgga aaatcttatc agctaagtta atcaaatcct ctttggaatc cgaagtcaag
    28321 cgtaggacag acattggcca cttgataatt ccagccttga cctgctcaaa accagcaaat
    28381 cggaaggtct tttgcaaggg agccaattcc ataggaaata cgtgacggcc tccctgataa
    28441 tgatcatgag ttagaataga gccccccaca atcggcaggt cggcattaga tccagcaaaa
    28501 tatcctggaa actgctctac gatagccaac agacgttcaa aactctgacg actaatggcc
    28561 atgggacgat gctggccatc taaaaagata caatgctcat taaagtaagc atagggcgaa
    28621 tactggaaac cccattcctg accaaccatt tcaaaacgga taatccggtg gttggtgcga
    28681 gctgggtggt tgacccgacc atggtaaccc tcattctcta aacaaagttg acactgagga
    28741 taattactag cttgcaccaa cttggctgcc gcaatctctt tcggatcctt ttcaggctta
    28801 gagagattga tggtaatttc aagttctcca taatcagatg gaacacgata agcgatattt
    28861 ttagcaatgg ccttgagttt gatgtagtca tttttctgac tgagttgata aaaatcctct
    28921 atcgcttgct caggagagta ggcataggct tcccagaagt cacgattgac ctgactcgga
    28981 caaggagtca ccaagtccat caattcagta ccaaggattt cacgcgcagt ctgactatcc
    29041 tcaatcgtct ctaatcgaac ggcttcctca actagctggt ccttgaggtc aatcaattta
    29101 tccagattgg tctcaacttc caaaacacct tctcccactc gtgccaaaac acgattggtc
    29161 aggtagattc gatccatttc ctcaaatgaa ctttcagaaa tgacatgtgt tacaaattta
    29221 tctactaagg tcactataga gcctcctttt gactagtcaa ggacgcgagt gccacctgca
    29281 acttcagcga tatagaagct tggagcgtat ccaactactt cctcgtagtg tttgcctaca
    29341 gcttccttaa aggcctcaac aatatctttt tgaaccaagg caatggcaca gccaccaaaa
    29401 ccagcccctg tcatacgagc accgagaact ccttcttgtg cccaagctgt gtgaacaagg
    29461 gtatctaatt ccaaaccagt tacttcataa tcatgctcca gagaaacgtg tgacgcattc
    29521 atcaaacgac caaatgtttc caaatcacct gcttgaaggg ctgcttgagc tttaagggta
    29581 cgttggtttt caagcacagc atggcgagca cgtttcaaac gattttcatc tttaatcaaa
    29641 tagctatatt ggtcaacggc ccactcgtcc aattcaccca gggtctgaat atccaaggca
    29701 acttgcaatt cttccactgc tttttcacac tcagcacgac gttcattgta tttagagtcc
    29761 gccaattcac ggcgtttgtt ggtgttcatg ataacaacga cattgtcctt caaatcaagt
    29821 ggcaccaaat catactctaa ggtgttggta tcaaggtaaa tagcacgttg atcagccccc
    29881 ataccaatag caaactggtc catgatacca gagttgactc cgataaagtt gttttctgtt
    29941 tgttttccga ttttaaccaa atcgagacgc tctaatttta aatcaaagag atgctctgcc
    30001 acgactcctg tcaagagctc caaggatgct gaagaagaca aaccagcacc atttggtata
    30061 ttcccataaa cataaaaatc aaaacctttg tcaatcacgt gcccagcttc ttgcaagaaa
    30121 tgaaggacac cttttggata attggtccag ttgtgctctt tttcaaactt gaggtcagcg
    30181 agaggcactt cgataatgcc cttgtcctca aagttagctg agtagaaacg taaaacttgg
    30241 tcttcacgct tgcgagctgc accataagtt cccaaggaaa tagcagctgg aaaaacgtgc
    30301 ccaccgttgt agtctgtgtg ttcaccaatc aaattgatac gacctggtga aaagaaggtt
    30361 tggtctgctt cttgaccaaa aacagcaaga aagtctttgc gaagagtttc agcagtaaga
    30421 tgttgtgtca tatgatttct cctttgactg tccgttaagc caccttaacg tgtaatcgtt
    30481 ttcttctatt gtatccaagg gatttcccaa ggtcaatcct ttttactaaa attttactaa
    30541 aatatcagta aaaagcagaa aaatatgata tactaaccta atcaaggagg atttatggct
    30601 accttaaaag acattgcaca gctagcctct gtctctatcg cgaccgtatc ccgtgttctc
    30661 aaccgcgacc agagcctatc tgttacagaa gaaaccagac accgtatttt aaccgttgct
    30721 gaagagctgg gctacaccaa gcacctcaag acaggcgagt cccataaacc caagcaaaag
    30781 attgccatta ttcaatgggt cagcgaacaa ggggagctag acgacctcta ctactaccag
    30841 attcgcctag gaatagaaaa aagagcccaa gagttggact acgatatctt gcgctatttt
    30901 aatgaccacc cttttaccct aagcgaggaa gtgattggga ttctctgcat cggaaagttt
    30961 agtcgagctc agatttctgc ctttgaagaa taccaaaagc ctcttgtatt tctagacagc
    31021 gatacacttt ccctgggaca tacctgtatt atcacggatt tttacactgc tatgaaacag
    31081 gttgtcgatt atttcctcag ccaaggaatg gaccgtatcg ggattctaac aggccttgaa
    31141 gaaacaacag accaagaaga aatcattgag gataagcggc tggaaaattt cagaaactac
    31201 agtcaagcga ggggaatcta tcatgatgaa ctggtctttc aaggaagatt tactgcccag
    31261 tctggctatg acttaatgaa ggaggccatt cagagcttgg gagaccaact tccgccagca
    31321 tttttcgcag ccagcgatag tttagctatc ggtgccctcc gtgccctcca agaagctgga
    31381 atcaacctgc cagatcgcgt cagcctcatt tcctttaacg acactagtct gaccaaacag
    31441 gtctatcctc ccctctctag tattacagtt tatactgaag aaatgggccg agcaggtatg
    31501 gatattctta acaaggaagt cctccacggt cggaaaatcc ctagcctgac catgctggga
    31561 accagactga cattaagaga aagtacccta aatcaagaat aggataacat aaaaaacgaa
    31621 taggagtttt agaactccta ttcgtttttt attcgattac aatcatagac ttaatggtct
    31681 tacgttcatc catatctttg taggcttggt cgatatcttc cagtttataa cttgaagtaa
    31741 agacgcgacc tggattgata tcaccatcaa ggacggcttt tagtaaaaat tgcttatcgt
    31801 atgttgtagc agaagctgcc ccacctgcta cagagatatt ttgcataaat gtcgaaccaa
    31861 gagcacgatt attatagtgt gggactccta caaagcccat acgccctcca ttatgaagaa
    31921 cacctagcgc ctgttctata gcagcctccg taccaacaca ttcaaatgct gcatctgctc
    31981 ctccaccgag gatttcacgc accttggtaa ttccttcttg accacgttct gcaacaacag
    32041 ctgtcgcacc tgactccata gccatctttt gacggtcttc atgacggctc ataaggataa
    32101 tttgtgatgc tccacgcatc ttagccgcga tgacagcaca ttgaccaaca gccccatcac
    32161 cgataacaac aaccttgtcc cctttttgaa catttgcaac acgcgccgca tgatagcctg
    32221 tcggcatgac atctgcaaga gtcaaaaggg acttgagcat cccttctgta tagtcagaag
    32281 gttgaccagg gattttaacc agcgcccagt ttgcatagtg gaagcgaata tattctgcct
    32341 gaaaatcacc ccccaaatta ttgccaatat gattgtcgca agaaccgtca aatccagcaa
    32401 gacaggcatc acactcacca catccatgtg taaaagggac aatcacaaaa tcacctggtt
    32461 tcaccgtcgt aatggcttcc ccagcttctt caacaatccc aatcgcttcg tgtccactat
    32521 ttttgtgtcc agctttcgtt tctggattac ggtacctcca taaatctgaa ccacaaacgc
    32581 acgcacgaac cacacgaata atcacatcat ccgcttctat tatttgcgga cgttcaatgc
    32641 tagcaagtcc aacctgacct gcctttgtat atactgctga tttcatttaa aattttcctt
    32701 ccttataaag tttaattttg agatttaaac gatttaaagc tgtctgtagc tgagacaagc
    32761 gctcctctaa tttttgcttt tcctcttcta aaataccaag cctctcctct ctcgtttcat
    32821 ctcccttttg gtagagcgac atatagtcaa ctaaactatc tacagagaca cccgccgaac
    32881 gaaaacactt aataaattcc agcgcttcga tatcctgatc ttgaaaatca cgaatcccag
    32941 tagcagtacg agtaatcggt ggcacaagac caacccgttc ataataccga atcgtatccg
    33001 ctgaaattcc caacaaatca ctggcagatt taatattcac acgctaacct cctctcattt
    33061 cattcactga gttcacgcta ttctaactca cttatcttat tatataactt tgagttgact
    33121 ccaagtcaag cattttgtat ctaatcgaaa aatttttatc attaacaatc atatatctgg
    33181 agtttataca atgctcttct cttaccaatt tttgaaatat ttaaataata aagaaattac
    33241 tctattccaa ataagatagt acccaagtat ttttcacttt tttctaatgt tgatgctcat
    33301 aactccgttc caagtcacac acctttcgct tatgagtttg atgagtttct aggtcagcat
    33361 caatttcaat ggtaatattt tgaaaaccac aatcttttag gaaaattcga atagactctt
    33421 tacaagtttc catatgttcc atttctttta gacaaacatg gacaatggca tttttttcca
    33481 aagcatccat agtccagaga ttaagctgat taaggctggc cacattatcc aatcgttcca
    33541 ggccactctt tacttgcttg atatcaagac cttctggcac agcatccaag aaaatcttga
    33601 gtgtagacca aaaacgtgaa agggcttttg aaagaataaa gaaagaaatg acaagggaca
    33661 aaagaggatc taggatatac cagtccgtaa atcgaagaac aatcgccatc aggataacag
    33721 ctacccaccc tagcgtatct tccagaaaat gcagattcag aatagactca ttctttgtct
    33781 ttcccttacc aaccaccaga ctcgctaaca gattgatagt aatcgcaata attcctaacc
    33841 agagaatccc ctcatcattg actggttgcg gatgcaaaat cttcgtgaca ttttccaaaa
    33901 tgactagaac agagcccgtt acgagaatca cagctgttac caaggctcct agcaggctaa
    33961 accgcttata gcccaaggtg tactgattgt cttcttcacg attggagatt gtttctagaa
    34021 aagctgatat tccaattgca atcgcatctc ccaagtcatg cacagagtca gcaagaacag
    34081 cgctagaacc aaatactcca cctgcaataa actcaacaat ggcataagtc aaatttaaga
    34141 aaaaagccac ccaaacagca tattttgcct tcatatttct cattcctttg ttataataga
    34201 tttatgaaca ccttgttcat tatcattatc cactaaaact caaagaaagg atagaagcaa
    34261 aacgtcagct ttattcagtt ctgaacaatt tgcctcaagt gtccatatga ctaacattga
    34321 ccgccgtatc agcaaaacca aaaaagccat ctatcaagct tttatacaac ttttgaatgc
    34381 taagggctac gaggccacta ctgttcagga tatcattgat ctcgcagatg tgggacgatc
    34441 caccttttac tgtcactatg agagtaagga gcttcttctg gaccagctct gccgctacct
    34501 cttccatcat ctttttgaaa gagagcaagc catttcaacc gaggactacc tcgcccacct
    34561 ctttctgcat tttcataaaa accaagacca tatcaccagt ctgctatttt ccaaaaatga
    34621 ctacttcctc cgtcaactcc ataaagagct agaacaccat gtctattccg tgctagctga
    34681 taatttaaaa gaagcccacc ctaatctgcc tacttcttac ctccaacact tggtcatgtc
    34741 caactttatc gagacattga cctggtggct caaaaaaggt caagatttca cagaccagga
    34801 agttgtccaa ttttatctag accttctcat tcctaaaaat tgaatataga gtaaagctca
    34861 gttgtcttat ttctagatta ctgagttttt tatcttttca acaacaaaag aggacccgcc
    34921 gatcctcttt ttcatactat aaatccttga ttatcaacta tatctgtttt aatcgaaatc
    34981 tcaaaacagc actttcaaac atcttttcct agttaagtaa atcagtattc tgcttagctg
    35041 ccttgctcca ttgataccaa ccaactagac tgttaatgag ataaattaga tatttccctt
    35101 gaatttgcag gctttctccc caccagagat agattgaaaa aacattggta gccgcccaga
    35161 atatccactg ttcacggtaa acagctgtca tgaggatttg ccctacccca ttggttgcat
    35221 ctgtgattga atcacgatag ggacgattgg caccaataga ctgataaatg aagccaaagg
    35281 ccaaccacca aagcacacta atggaaagat actttgtcca gcccttgccg tccagtttac
    35341 gcgcgacaaa ctcctgcttt tccttcttaa actgtgcctg ataaatccaa actagaagtc
    35401 caattggctg catgactgtg aagtaaagtg tcgtcagcac ctcaccataa aagcctttct
    35461 gtagggccaa aataaggtaa ataacagagt taatcaagcc aaaaagataa ttacttgctc
    35521 gaccttccga tacaaagatt acacagataa tccctgtcaa gctacaaatc atcccaatcc
    35581 agtcaacaat acgatgttcg taaaccaact ccagccagag aggaaaactt cctaaaacca
    35641 gcaaataaat ccactgggca aaactacgat gggcaaagag gtcatcccag atagccttca
    35701 tagttcctga aaatcctaaa tcagccatag ccgcaaccat acgacggtaa ccacctgaca
    35761 tttcacctag ggttgttttg atattttcaa ttttcttttg caaataagta tgcatcattt
    35821 ctccttttgt ttttaaagag ccgtgtctgg atagactttc ggacgcaacg ctctattaga
    35881 taatgaactg cctatacaca agatttctaa ccttagtcga catgagctga aacctcttat
    35941 ttgttaagta gttcacaaaa tattatacac ctattttatg aatagtcaac tgtctttaca
    36001 gtaaaatttt agaaaatcat gaaaattttc tctttctttc cattttaagt gacattcagt
    36061 cattctcaca tcaaaaaagc ccagacgaaa ttgtctgagc attcttttat ctagtcgttt
    36121 aaggaagttg agttcagtat gtttaaagtc tctgtcccat catttcttca acaaaccttg
    36181 ttcttgtaga aattccttgg ctacttgttc tgctggcttg ccttcgacac cgacttgata
    36241 gttaagctgg ctcatctggc tttctgtaat cttaccagcc aatttattaa gaacagtttc
    36301 caattctggg tgtttcttga gaagagcttc tttcatcagt ggcgccccct gataaggtgg
    36361 gaagagttgc ttgtcatctt ccaagacctg taaatcataa cgctccaatt ccgcatcagt
    36421 cgaataggca tctgtaattt gaatttcacc tgactgaatc gcttgatagc gaagagcagg
    36481 ttccaaggtt gaaacattga gattgagacc atacattgat tgcaagccct tatttccatc
    36541 ttcacggtcg ttaaactcga gtgtaaagcc tgccttcaac tgaccttcca cttttttcaa
    36601 atccgaaatg gtcttcaagc catattcttg cgcaatcttt ttcggaacag ctacagcata
    36661 agtattttga taagacatgg gtttaagata ggctagatga tcctgtttgg caatgccatc
    36721 acgcgccacc tgataaacct gctctggctc atgactcact ttaggtgatg gttgaagcaa
    36781 actttcagtc accgtaccag taaattcagg atagatatca atgtctccgc ttttcaacgc
    36841 ttcataaagg aaagttgttt tcccaaagtt aggcttaacg gttgctgtta gacttgtatt
    36901 ttcttctatt aaaagtttat acatattagc caaaatttct ggttctggac caattttccc
    36961 tgcaataatc acgttttctt tctctttttg aaccaagagg gctggactat aagatagacc
    37021 cagcaatacc gtcaccaagg caaaacctga gaaaatcgtc cgtaattttg ctttttccat
    37081 tacttttagt aggaagttaa aggcaatagc aagcactgca gaagaaagtg ccccaatcaa
    37141 aatcaaactg gcattattac ggtcaattcc caacaggata aaggaaccca gtccccctgc
    37201 tccaatcaag gccgccaagg ttgccgtacc gataatcaaa acagctgccg tccgaatccc
    37261 agacatgata acaggcatgg cgagtggaat ttcaaatttc ttgagacgtt cccatctggt
    37321 catcccaaag gcaatcccag cctcttgcag gttcggatca attcccttca gcccagtgat
    37381 agtattttgc aaaataggga aaatcgcata aatcactaga gctgtcaaag ccggcaaggt
    37441 cccaattccc atcaaaggga taaagagccc caacaaggcc agagacggga tggtctggaa
    37501 aatacctgca atctgcaaga cccagtcggc cagcttctca tgatagcgaa gaaaaacagc
    37561 caagggaatc gcaagcaaaa tagctagtaa caaggtcaaa agtgacaact gcaaatgttg
    37621 agatagagct gtcaaccaat cactaaaacg atcctgaaaa gttgcaatta aattagtcat
    37681 gaacactacc tccaaacaag tctgctacaa agtctgttgc aggcgctttt aaaattgtct
    37741 cgggattcgc tacctggcga atttctccat cctgcaagac agcaatacgg tccgccaact
    37801 tcaaggcttc atccgtatca tgggttacaa aaatcgttgt catcccaaac tctttatgca
    37861 attcttttgt cagaacctgc aactgttttc tcgaaatagc atccaaggcc gaaaagggtt
    37921 catccatgag gaaaatcttg ggctgaccaa tcatagctcg gacaataccg acccgttgct
    37981 gttctccacc agataattca ctaggtaagc gatgcccata ctcggctact ggtaaaccaa
    38041 ccttagccaa aagctcttct gttttcttcg taatttcttc cttgctccac cccttcattt
    38101 caggaatgag agcaatattt tccgcaactg ttagatttgg aaaaagagca atagcctgta
    38161 aaacataacc agtagaaaga cgaagttcac gctcatcata gtctttgatg cgcttcccat
    38221 ccatataaat atttccatca gttggttcca aaagacggtt aatcatcttg agcatggtcg
    38281 tcttacctga cccagaaggc cctactaaaa ccataaattc cccatcctca atctgtaagt
    38341 tgacatctct caagacatcc ttttctgtgt agcgcagtgc tacatttttg tattcaatca
    38401 ttctttgtcc tcaatttaaa acttccctcg attggtcaag tcttctacct taggcatgac
    38461 ttccttatta tcccaatgct ccacaatttt cccgttctct aaacggaaga tatcgtactg
    38521 ggcataagca acgccatcaa tctgagtctg accatagcta accacatagt ttccttgtcc
    38581 taagagttgg aaaacaaagt caaaagtgac actatattca gccacatagt ttttataagc
    38641 agcacttcct tgtccaatat catgattatg ctgaatcaaa tcgtctgcca cataatcact
    38701 ccactgctct agctccccat tttggaaaat ttctgtcaag aaacggcgaa ccagcttttt
    38761 attttctgct ttcttgtcca aatccttgat ttcaaaatct ccaaaaattt gatctagttg
    38821 gtcattttca ggtgttcgat agtagtcaat gacatcccaa tgctcaacaa tacgaccatt
    38881 ctcatccgca cggaaagtat ccgtcgtcac ccattgagct tctccaccat tcagatattg
    38941 atgaacatga acaaagacca gattgccatc ctcaatggtg cggacaatct taatctgacg
    39001 ctctggatga cgctcaaaga aatctgcaaa gaaggctgca aatccttctt tcccgtcagg
    39061 aacacctgtc gaatgttgga tataggtatc ccctacagac tgggcttgag cctcagcaac
    39121 tcgtccgtct tgaatggcat ggatgtatag gttgtgagca tttttcactt gttgtgacat
    39181 attctaaacc tcatttccct tctctttcag attcgccaaa attctttctt gaaaaccttc
    39241 aaattggtga atttcttcct ctgaaaatcc tttgtaaaag atagtatcca atttctgact
    39301 gacacgatgc cccacttctt tctgggactt gcctaactcc gttaaaacta aatacttctt
    39361 acgcttgtct tttccacacg gactaacaat tacaagcttt tgttcctcta gcttttttat
    39421 catagtcgtc agcgtattat tcgcaagtcc agtcgcaagc gcgatatctg tcgcagttgc
    39481 gcagccagtt tcactattcc ataaaaccgc taaaatcttg ccctgttcac ccctataaag
    39541 agcctcagga tcttgactca gtaacttttg aaaaatccgc ccattcaaca aacgaatatg
    39601 atgggctagc aaatgaccat ctttcataac acctccaatt tatttcgata tcgaaatgaa
    39661 taaaacaatt gtaacactca tcgttctaac tgtcaactat ttcgatttag aaataatttt
    39721 tgataattat ccacaccacc atactccggc tcaactaact tttaacgaga gtttctaaac
    39781 tccttcgtcc tccagtctac aaaagccttc catccgtact atcctatatt ttatgagggg
    39841 acacattttt cctatcagag catttatttt aaagatagaa gtaaatcata attgcttcca
    39901 tctgttcttt tatagtatat tgaagttgga ctagggcact gtatcttcta aaacattgat
    39961 agaaagcgat ttgaatttcc caatcaattt gttcgtcttt atagcatttc gaaactggaa
    40021 taggacacca tgactgctaa aagatttcta taaattcatt tgatttccct aatcaatttg
    40081 ttcatatctt atttcattcc gctataattt caccttaccc tatctttttc gtagcaccct
    40141 tcaaacagcc tatcccctac cgtttgacga ttcctcactt cactccactt ccattacaga
    40201 agtttcttca ctactatggt ctcggctgac ttctcatgat tccttgttac tactatttga
    40261 acgctcacga gataggtctt ataaaaaatg ctttgatcca caatggaatc aaagcatttt
    40321 aaaaagttcc tcatacataa gtgcagaagt cgcagttcct ctgtacttgg cttcttctct
    40381 tttgataaag cgagccaagt tgagcaactc aggtgctgga tgtttgggat ttaggagcaa
    40441 ttcacgattg accaggcctg agagacgaac tgcctgcaat tgctcatttg tagtaggcag
    40501 ttttttagta gtctctagga gagcagcaac taaatcttca ctcaaatcat gtcgagcatg
    40561 attgtaaaga tcttttataa ggctttctag gtttggttct gccatcccta ccacctccct
    40621 tatggtttaa taatgtttaa tcaaatcaac cgttgaacga tccaatttct tcaccaaggc
    40681 ttgtaagaaa gcttgcgctt ctaggaagtc atccattgca tagagggttt ggtgagaatg
    40741 gatataacga gcgcagacac cgatagttgt tgatgggaca ccaccatttt tcagatgagc
    40801 tgcacctgca tctgttccgc ctttaccaca gtagtattgg tacttgatac cagcttcttc
    40861 agccgttgtc aaaagaaaat ccttcatccc tgggagaagc aagtgacctg gatcatagaa
    40921 acgaatcaag gttccatctc caatcttgcc ttgaccaccg tagacatcac ctgctggtga
    40981 gcaatcaact gcgaggaaga cttctgggtc aaacttggtt gtagaggtat gagcgccacg
    41041 cagaccaact tcttcttgga cgttagaacc cagatagagt tcattgccga gtttttgacc
    41101 tgataaagct tcagctagct cgcttaccat gaggacaccg tagcggttat cccaagcttt
    41161 tgagatgata tttttttcat tggctgtcaa aattgcagaa ctatctggta cgatggtatc
    41221 accaggacgg atgccaaaac tttctgcctc atccttgtcc gcaaaaccac catcaaaaac
    41281 gatatcggca atggctggca tggttggtcc cccctttcca cgagtcaaat gcggaggaac
    41341 agaacctgaa atcacaggaa tttcatgacc atcacgagtc aagagtttga aacgttggct
    41401 gctaactacc atggggttcc agccaccgat ttctacgaca cggaaggtac catctggctt
    41461 gatttcgctg accataaaac caacttcgtc catatgagaa gcgaccaaga cgcgcggtgc
    41521 atccacagct tctgaatgtt tgataccaaa aataccaccc aagccatctg tcaccacttc
    41581 atccacatgc ggtgtcaact tttcacgaag ataagcacgg acaggcgctt catgacctga
    41641 gactgcagca agttctgtta cttctttaat ttttgaaaat aatgttgtca tttcagttcc
    41701 ttctttcttt catccatttt accacttttt ataggagaag gatagtgaga aggtggattt
    41761 ctaagttagt atcttagtcc tgctctatct tagaaaagga tagtattctc ttgcatgtag
    41821 tgcaaaatct agtaaacatt ccaaaattaa ctcgaatatt tatttccaaa caaaaaaaca
    41881 atacaccatc aaagttgttt ggatttttca tgaaatttac agaaaatagt tgacttccct
    41941 ttcttctttc tttaaatata tagttggttg agtttggaat agtacgctgt agctgctgaa
    42001 acatttctag aaattaattt gactttccta atagagttgt tcatatctta tttcaattta
    42061 ctatagtaca aaactagaaa aggaaaaaat catgaccaaa acaattcttg ttacaggtgg
    42121 gactagctac attggctccc ataccgttaa agctctttta aatgctggtt ataaggtgca
    42181 tgtcctcgat aatctctcca caggaaatcg agcgactgtg gataatcgtg ctagctttaa
    42241 agaactggat gtttatgatg ctagcgcctt aaaggcttac ttagaagaaa atcagattga
    42301 tgctgtcctc cattatgcag gtgaaattgt tgtgagcgaa agtattgaaa atccaagtaa
    42361 atacttcact gccaacgttg ctggtaggaa ccaagttctc aaagtcttat ctgaagttgg
    42421 cactcaaaaa atcatgttct cttcgactgc ttctctctat ggaaataact gtattgacaa
    42481 gccggcgact gaagataccc tgatcgaccc tgtcaaccct tatacagaga caaaactgat
    42541 gggcgaacga atgatttact ggatggccaa tcgctacgac tggaaatatg ttattttccg
    42601 ttactttaat gttgctgggg ctgaaatgga tgcttcaaac ggtctgcgtg tgaaaaatcc
    42661 aactcacatc attccaaata tcaacaagac cgcattggga caaaatgata gcttaaaaat
    42721 atttggagat gactacgata cacgtgacgg ttcatgtatt cgagattaca tttatgtctt
    42781 ggaccttgca taggctcatg ttaaaggaat gaactaccta tttcaagaag acagttcttc
    42841 tcaaatcttt aacttaggaa ctaaaaaagg ctatactatc aaagaaatct ttaaaactgc
    42901 tgaagaatta ctaaattaaa aaatcccaca cgagattgtt gctcgacgtg ctagtgaccc
    42961 agccagtgtc ctagcagatg cttcaaaagc taaacaatat cttgattgga cggctagcta
    43021 ctccctcgaa gatattattt tatcagatta aggtaaaaac attttagaat aggataacaa
    43081 aggagaaagt cttgttgctc tctccttgtt gtatttatta aattgtcaga aattttattg
    43141 acagattaga aaaataatgt tacatttata tccgcagata tctttcgata ccaaatctac
    43201 atgaaaggac ggggtatgaa actttctcat tatttaattg gcttacttct actcctagtc
    43261 tttctctcta ttagcattgg gaccagtgat ttttcatggg gaaagctatt tgatttcgac
    43321 cagcagacct ggctcctctt tcaagagtcc cgtctcccaa gaactatcag tattctcctg
    43381 actgcctcta gtatgagtat ggcaggcctt ctcatgcaga ctattaccca aaatcagttt
    43441 gctgcaccga gtacagttgg aacgactgaa gccgccaaac tgggaatggt gctgagcctt
    43501 tttgtctttc catcggctag tctgacccaa aagatgctct tcgcttttgt ttcatccatc
    43561 gtattcaccc tcttcttcct agcctttatg accattttta ctgtaaagga aaggtggatg
    43621 ttgcctctga ttgggatcat ctatagcggg attatcggct cagtcacaga agttatcgcc
    43681 tatcgtttca atctggttca gagtatgact gcctggaccc agggctcctt ctccatgatt
    43741 cagacccatc agtatgagtg gctcttctta ggcctcatca tcctgataac cgtttggaaa
    43801 ttatcccaaa ccttcaccat catgaatcta gggaaagaaa ccagcgagag tttggggatt
    43861 tcctactccc tacttgaaaa actggccctc tttctggtgg cgctaacgac aagcgtcacc
    43921 atgattaccg tgggtggctt accatttctc ggagttatcg ttcccaatct tgttcgcaag
    43981 cgctatggag ataatctaag tcaaaccaaa ctcatggtcg cactggttgg tgccaatcta
    44041 gttctggctt gcgatatcct atcccgagtt ctgattaggc cctatgagtt gtctgtcagt
    44101 ctcttgctag gaatcatcgg tagtctcgtc tttatcctac ttctctggag agggggacga
    44161 aaagatgcag actaaaagca aacataccaa gctcttctgg attctcatta ttcttgccat
    44221 cggagcttgt cttctctact tttggcccat cactcacttg tcagcctttg cttggaagtt
    44281 gcgttcccaa aagatcatcg tttatctctt ggtagccatc gcgactggga tttcgaccat
    44341 tagttttcaa aacctgacgg aaaatcgctt cctgacgcct agtattttgg gaattgaatc
    44401 cttctacgtc ctactacaaa ccctactact ggtttttgaa agcaagtttc ttcaacttgg
    44461 caaatcccct atcttagaat tcctagtctt acttcttgtc cagtccctct tctttctcgc
    44521 cttacaaggt tacttgaaga cactgatgaa gcaagacctg gtcttcatcc tgctgatctg
    44581 tctagcgctc agaagtctct ttcgaaatat cagcaccttc cttcaagtcc taatggatcc
    44641 aaacgaatac gataaactgc aaaatagtct ttttgcctcc tttcaacatc tcaacacttc
    44701 catcctagcc atcggttctc tgatcatcct cgctttgaca atctttttct ttcgaaaagc
    44761 agtcgttcta gatgtcttgc acctgcaaag agaaacggct cagatattgg gactcgatgt
    44821 tgaaaaagaa cagaaagagc tcctctgggg aatcgtgctt ttgacctcaa cggccactgc
    44881 cttggtagga cctatggcct tcttcggctt tatgctggcc aacctcacct acctgattgt
    44941 caaagactat cagcacaagt tactctttat agtggccatt ctggttggat ttattagctt
    45001 aaccttgggg caatccttga ttgaacgagt ctttgcactg gaaattcgta tcagtatgat
    45061 cattgagagt gtgggtggct tcttattctt tatcttacta tataggaggt ctcgtcagtg
    45121 aaactggaaa acattgacaa atccattcaa aaacaggata ttttgcaagg catttcgctt
    45181 gaagtcagtc ctcaaaaact gacagccttt attggtccaa atggtgctgg aaaatcgact
    45241 ctcctctcca tcatgagcag actaaccaag aaagatcagg gagttctcag tatcaaagga
    45301 cgtgaaatcg agagctggaa ttcgcaagaa ctggcccaag aactaaccat cctaaaacag
    45361 aaaatcaatt accaagccaa attgactgtt gaagaactgg tcagttttgg acgttttccc
    45421 tacagccgag gtcgacttag atcagaagac tgggaaaaaa tccgagaaac tctgaactat
    45481 ttggaactga ccaacttaaa agaccgctac atcaatagcc tgtcaggggg gcaactccag
    45541 cgcgtcttta tcgctatggt actggcccag gatacggact ttatcttgct ggacgaacca
    45601 ctcaacaatc tcgatatcaa gcaaagcgtc agcatgatgc agattcttcg acgactggtg
    45661 gaggaactcg gcaagaccat tatcatcgtc ctccacgata tcaacatggc cagtcagtat
    45721 gcagatgaaa ttgtcgcctt caaggacggc caggtcttta gcaagggaag aaccgatcaa
    45781 atcatgcagg ctgacctgct cagtcaactt tatgagattc ccatcacgct agctgatatc
    45841 aatgacaaaa agatctgtat ctatagctag taacataaaa gctcaagtta gagaaccttc
    45901 agtctcttag tcaataagat taagagactc cctaaatcgt tatcacattt taaaaaggag
    45961 aaattatgaa aacatccctt aaactttatt tcactgccct agtggccagc ttcttgctcc
    46021 tacttggtgc atgtagtaca aactcaagca ctagtcagac agagaccagt agctctgctc
    46081 caacagagat aaccattaaa agttcactgg acgaggtcaa actttccaaa gttcctgaaa
    46141 agattgtgac ctttgacctc ggcgctgcgg atactattcg cgctttagga tttgaaaaaa
    46201 atatcgtcgg aatgcctaca aaaactgttc cgacttatct aaaagaccta gcgggaactg
    46261 tcaaaaatgt tggttctatg aaagaacctg atttagaagc tatcgccgcc cttgagcctg
    46321 atttgattat cgcttcacca cgtacacaaa aattcgtaga caaattcaaa gaaatcgccc
    46381 caaccgttct cttccaagca agcaaggacg actactggac ttctaccaag gctaatatcg
    46441 aatccttagc aagtgccttc ggcgaaactg gtacacagaa agccaaggaa gaattggcca
    46501 agctagacaa gagcatccaa gaagtcgcta ctaaaaatga aagctctgac aaaaaagccc
    46561 ttgcgatcct ccttaatgaa ggaaaaatgg cagcctttgg tgccaaatct cgtttctctt
    46621 tcttgtacca aaccttgaaa ttcaaaccaa ctgatacaac atttgaagac tcacgccacg
    46681 gacaagaagt cagctttgaa agtgtcaaag aaatcaaccc tgacatcctc tttgtcatca
    46741 accgtaccct tgccatcggt ggggacaact ctagcaacga cggtgtccta gaaaatgccc
    46801 ttatcgctga aacacctgct gctaaaaatg gtaagattat ccaactaaca ccagacctct
    46861 ggtatctaag cggaggcgga cttgaatcaa caaaactcat gattgaagac atacaaaaag
    46921 ctttgaaata agccacttca aactcaattt aatactcaat gaaaatcaaa gagcaaacta
    46981 ggaaactagc cgcaggttgc tcaaaacact gttttgaggt tgcagatgaa agctgatgcg
    47041 gtttgaagag attttctaag agtataaaat tttgacattc taaaacgcat cctatcaagt
    47101 tcactcaaac ctgataggat gcgtttttat cattttgaaa tctatttacc ccacctcatt
    47161 ccccttcttg atttcgttta agggaaaagt ggtctggaac gggatcctta cctgttttcg
    47221 accagggatg acaacgtaaa atccgagcca agcccatcaa taccccctta aaaccatgtt
    47281 tttcaatagc ctgaatcatg tagttggaac aagtcagctc aaagcgacaa gagggtggaa
    47341 agactggtga gataaaacgt tggtaaaagc gcacaggcgc tattaaaatt cgtttcatta
    47401 ttttttagtt acagccatgg tgtgtagttg gctgatttct tttttattaa gacgacggga
    47461 ttctcctgga cggagtcctg tcaagtctag gtgtccgaaa cgagtccgag acaacttatc
    47521 tacttggaga ccaacagctt caaacatctt tttaacctga tggttacgcc cttcatggat
    47581 ggtcaactgc accacagagc gatttttgac tgggtccact ttgagaattt cataaacagc
    47641 tggcttggtt ttcttaccat caatctcaag accacgggtc aaggggcgaa gattgtcctt
    47701 attggccaca cctttaacac gcgcgacata aaccttgtca atctcattac gagggtgaat
    47761 catctcgtct gtaaagtccc catcattggt caaaatcaag actcctgatg tatcccagtc
    47821 caaacgaccc acagggtaaa tacgctcttt gacattgggc aagaggtcga caaccgtctt
    47881 gcgaccctta tcatctgtca cactggaaat gacaccgcgt ggtttgttaa gcagatagta
    47941 gaccttttct tcgttgtaga taggttgacc ttcaacttcg accttgtcgc ctgacttgat
    48001 agtggttgct agttcacgca ccacttgacc gttaaccgtc accaagcctt gcttaatcag
    48061 ctcttctgct tttctcctac tggccacacc tgcgtgggca atatacttat tgattctcat
    48121 cttcttctat cctttcacca aataattggc tttcttgggc ttgaatctca agctcatcaa
    48181 tcactggtaa ttcttctaaa tggtttatcc ccatgtaatc taggaaataa tctgtagtca
    48241 catagaggtt ggggcgcccc aatacttcct ttttcccgtc ttcctttatc aggtcaaaag
    48301 cctgcaactt tgccaaggct ccactcgagt taactccacg gatggcatca atttctatcc
    48361 gcgtaatcgg ctgtttgtag gcaataatgg acaaggtctc aagggcagcc cgagacaagc
    48421 tctggttgat aggcgcctta gagtattcct tcaaaatctc tgcaaattga ggcttggtca
    48481 ccaatctata agcaccactt gtctcaatca aagccaaact ggaatctggg tccttttcat
    48541 acttctgggc taattttcct aaactttgct ggatgcctgt cggtggcaga gagaggagtt
    48601 cagctaactg gcggacccga atcccatctt cacccgctac aaacaagagc gcttctattt
    48661 ttgctaaagt actcatcttt cctctctatc aagtctagct ttgaggcact tgactttctt
    48721 ccttcttttc catgagatag atatctccaa aactctcctc ttgcacgagg atcagctcct
    48781 gggttttgat taactctagg gttgccaaaa agagggtgat gacctcttgg acattctggg
    48841 cttccttgaa caaatcctgc aagcgcaatt gatctcgtcc aatcaaggac tctttcacga
    48901 taatcatcat gtcctcaatc ttatactcat cccgcaagat cgtcgtgtga ttttgtgcaa
    48961 actcctcttt tttcttggct aggatatttg aaaaagccaa aaagaggtca atggtcgtct
    49021 tgtcatgcac aagctccgca tcttcgtaaa tcaactctgt cggcgctttg gaataatact
    49081 gggcccgttc ttggtgcttg gcttccaagt gctcacccaa gagcttgaac ttgcgatatt
    49141 cttcgatttg agagaggagg tcctgctcca ggtcatcccc caagtctgtc acttctgcta
    49201 ccttcggaag gagtttacga ctcttaatca gcatgagctg actagccatg accatgtact
    49261 cacccgtcac ttccagacgc atggcctgca gggttgagac ataggctaga tactgttcga
    49321 tgacttccgt aatgggcaca tcgtagatat ccatctggta cttagaaacc agatgcaaga
    49381 gtaagtccag gggtccttca aaatctttta atttaatatc cattatctat atttttctaa
    49441 ggtcaggact gtttttaatc ctaatttttt tgcaatttcg tacaaatcga ccttgttttc
    49501 tatttgtctt agaataaact gttcacgtaa gacttgagct gataaggatg gaaaaccttt
    49561 ctccttgaca aaagattcta actgacgaaa ggcccactga cgagaatagg gtttccctcc
    49621 tctttcaaaa agataggtct gccccatcaa gggttccaat tctgaaagca aggccgtggg
    49681 aatggtgaca atcctccgtt gggaagcctt gctgattcgc aacacctgaa aatccagatt
    49741 gatgtccgca accttgatgg ctaaaatctc actgggcaag agccccattt ctaggattaa
    49801 gagcgctagc aagcggccct ctggatggtc gctttcctgc caaaaagagt ctaggtatag
    49861 aatctctggc ttttccgtct tcttttcagc ttgtttggct aattccaagc ggtaaaagct
    49921 gtccacctct cctttttgat agagaaagta tagaaattgg ttacaggccg aaatctttcg
    49981 cttctgggcg ctgattttta gattggctag ctgggcttgg taaatcttga gactggtctc
    50041 agaaatccgc tcacctacca tgtctaaaaa ttgctccaaa tcatacttat aggactgctt
    50101 ggaattgaca gacaagccct gcttttcctc taaaaaggct gaaatcctgt ctctcattgg
    50161 catcgaatct catattcctt actaaagtca tgcaagaggg cattaacggc cttgaggatg
    50221 gacttgcgcg taataatccc ttggaaaata ccctctgcat ccacaaccgg taagaaggac
    50281 tcatctacta gcttgtgcaa gacctccgta atggtgaaat caggcgaaac aaccgctacg
    50341 tccgtttttg tcatatgaac gatatccgta tccgccatga tttcttggct caagtcatgc
    50401 tccatctgat aagccataat atctctgagt ccaatcgtcc caacaaactg tttttcatct
    50461 gtcacaacgg gaacacgggt ataggtcatc tgactgagca agagggtcgc atgatccgca
    50521 ttgtgggtat caatcaacac agctagattt ttagcagggg tcaaaaaagt ttcctcctgc
    50581 cccaacaaga aagtctcaaa ctccttggca atcatcggct aaactccttg gacaaacctg
    50641 gatacacctc gtgatctcgt gtcaaaaagt ccactttgaa gtaactatca tcaatctcca
    50701 cacgagcata gagacattct ctgatggtac ctcgtggttg gctgatagaa cctggattta
    50761 gaaagaggat cttgccttcc atccaagcac ttggcacatg caagtgacca tagaggcaga
    50821 tagcggcctc ttcctcctga gcccagtagt ccaacttttg aaagttgaaa ttgatgtcaa
    50881 acaagtgacc atgagtttgg ataatcttgg tcgaaccaag ctcagtcacc agacgttctg
    50941 ggtagccggc gtagaagtcc atgttccctt taacaacgcg gatgccctcc caaagtggag
    51001 aatccggacg tagttcagaa tcgccgttat gaaaaacggc atcgactttg cccacatagc
    51061 gatcacggac ttcttccaca atcaagctat cgccatggga atcgctcatt acaatgatgg
    51121 tttgctttgc catgatggaa atacctccaa aagtttctta acggctaagg cacggtgaga
    51181 ttgactattt ttttcttcca gggttaattc agctgatgac tcacctgtct ctcctacaag
    51241 gaagagggga tcatagccaa agccattttc acccttaggt tcaaagttaa tataacctga
    51301 ccagtctgct tcaacaacta aactttcctt atttgggctg gctacgacta gggttgtgtg
    51361 gaactgagcc gagcggtcct tgagttcaaa gaccatggcc aattcgtgca agagtttggc
    51421 attattttca cggtcagttg ctcccacacc tgcgaaacga gctgaccaga cgcctggtaa
    51481 gccaccaagg acatcgactt tgagaccaga atcatctgcc aaaaccatct tgcccgttaa
    51541 ttgagaaatg gtttctgcct taaggcgggc attttcttca aaggtcatac ctgtttctgc
    51601 tacttcaggc aggtcagggt agtcattaag attttccaca tcgtagccta acttatcaaa
    51661 gatagctcgg aattccttgg ttttaccttc gttacgagtc gcaatcaaca aggtttctct
    51721 gaccttgctt gtctcaaaga aatcatgaac attgacccct tctttaggca attctacata
    51781 caagagttgc ccattttcaa ccaagagcag ggccccatga cgttgaataa cttccaagtt
    51841 tcgtcccatt tcttgattaa gcatgtctac cacaaaggag aacaaacggt aggtagaacc
    51901 atagcgtaaa acagtaactg aaagcgggaa accatactct tcatctccaa atattttgga
    51961 gaattcaaac agaggaaaat ctgtcttata gtcgctcaaa ctgttaacgc caccaaaaat
    52021 actataagac ccaacatacc agtcctggtc atccttatat tcataaattt tatttgtcat
    52081 aattctacat gctccacatg aatctctttt tccagccatt cttcaccaat ttgtgcaaaa
    52141 ctttggctac tggctgttgt gtaaaaacgg tgatggagtg gtccagcatc gcgaccacga
    52201 ttgatttcaa aataattgag taagactgag atatcccgta cgcactctgc cccactatcg
    52261 atgagctgaa cctttggccc catcacattt tggataatag ggcgaaggag tggataatga
    52321 gtacagccca aaatcaggct atccaccttt ccaaccaagg gacgcagggt ttcatagacc
    52381 accttcttgg taacactggt tgacagggca cctgactcaa ccaagggagc aaacttggga
    52441 caggccaagc tctccacctg taagtcggga tccagatcat ggattttctg acggtatatg
    52501 tctgattgta ccgtcatggg cgttccaatc actccgattt tcccaccttg actggacttg
    52561 atggctgccg aagctcctgg caaaattaca cccaagacag gaatatctag ttgagccttg
    52621 atttcttccc agacgaccgc agtcgcagtg ttacaagcaa tgacaatcat tttgacatcc
    52681 ttggtcaaga gaaagttgac cagctgccaa gtatattcac gaatttgctc agcaggacgg
    52741 gggccatagg gcgcccgcgc cgaatctcca atatagacga tttcttcatg gggaagctgg
    52801 cgcatgagct cgcgcacaac ggtcaagccc ccgacacccg aatccaaaaa accaattggt
    52861 cgattatcca tacagtcttc tttctagtct tttttctgat tgatcgttca ttatgattcc
    52921 ttgtactgaa cgaactgacc gacagcttga tagaatgtca actacctctc tcttaatcat
    52981 aatcaaatat caatagaatg caaggaaaaa gtctcatccc taaaccataa ccaacatagt
    53041 gagaagtctg gaactttcat cccagacttt tccttattta ttttttcttt ttattgttag
    53101 caagggctgc cttttgttgg cggatgattt ggtggtaaac ttgttgtacc ttagcttcgc
    53161 ttggtttttg accatttgca ctcaaaagag tacgaactgc ttcagcattc aaacgtgggt
    53221 tgtcagcaaa ttctttttcg atttgcttac gaaccaagta cattcctcca agagctcctc
    53281 ctagaaaagc tagcacaatc aatacaattg ctaaaagtaa atccatcatt tttctcctgt
    53341 ttctttttga gtccctttca ttataccaca aactagccac cctgaccagt ttgttctaca
    53401 ctttcaggga tggaatagac agcaaaaaga accctgcttt tctgaaaaag aggtctcctt
    53461 gctaagttta tactcaatga aaatcaaaga gcaaactagg aagctagcca caggttgctc
    53521 aaagcaccgc tttgaggttg cagataaaac tgacacggtt tgaagagatt ttcgaagagt
    53581 attaatttac ataaatagcc agtgtttgat agggtttgag tagaattttc tcagacactt
    53641 ctgcatcttc atagtttgat atcaaaatct gtccattttg gtagactgct ggcaagtcga
    53701 tttccacttc tttagcataa aagttattga gcactagtaa cttttgatcc tcaaactggc
    53761 gttcaaaagc gtagacttgt ttgctatctt caaaggctgg tttgtaactt ccttctgaaa
    53821 tgattggcat ttccttacgc agtcgaatca agtcttgata gaaggtaaaa atcggaccct
    53881 ggatttcatt ttctacattg atgtatttat aggatttacc agctttcaac caaggagtgc
    53941 ctgttgaaaa tcctgcattt tccgaagcat cccactgcat gggaatgcgt gaattatcac
    54001 gcgacttagc ttgaataatc tggaaggctt cttgctgact ctttccttct tctaagagca
    54061 tctgataggc attaagcgat tcgacatcca cataatcagc catagaatca tagtctgggt
    54121 caatcatccc gatttcctca cccatgtaga tataaggtgt cccacgtgac aggtgaatgc
    54181 tggctgctag catggtggct ccttccttgc ggaagttttg aatatcgaca aaacggttca
    54241 aggcacgtgg ttgatcgtga ttgttccaaa agagggcact ccaaccgtct ttatcactca
    54301 tttccttacc ccaactatgg taaagactct tcaactcttc aaaatcaaag ggagccaagg
    54361 tccacttttg tccatcctta tagtccacct tgaggtgatg aaaattaaag gtcatggata
    54421 attcctgacg atcaggcgac gaatagagga cacagttttc catggtggta gaagacattt
    54481 ccccaactgt cataaagcta tcgtcagatc caaaagtggc ttggttcatc atacgcaaat
    54541 agttatgaac gatgggtttg tctgtataag ctggcttccc ttcattttca ggacagtcca
    54601 ctgaaacctc gtccttaccg atcaaattga tcacatcaaa tcggaaacct ttgacaccct
    54661 tgtcgcgcca gaaattaaca accttgaaaa gctccttacg gacattggaa ttgcgccagt
    54721 taaggtcagc ctgggtctca tcaaataggt gaagatagta tttcccagta tccccgaaag
    54781 gcgcccatgc agaaccgcca aacttagact gccaatctgt tggttggtct tggatgaaga
    54841 aaaagtcttg ataatactta tcaccagcta gggctttctg aaaccattca tgctctgtcg
    54901 aacaatgatt aagtaccatg tccagcataa agtcaatctt gtgctcttta ccgatacaca
    54961 ccatttcctc aaaatcagcc atatcaccaa aaagaggatc cactgccata taatctgaaa
    55021 tatcgtaacc attatcccgt tgaggacttg gatagaatgg attgagccag accatatcca
    55081 cacctagttt ggctagatag ggaatttttc cgataatccc acggaaatcc ccaataccat
    55141 tgccagtggt atctttgtaa gattttggat agatttgata gactactttt cctttatcaa
    55201 gtgtcatctg tttctccttt tctgataaaa gggaggaagc agtcttccgt ccctatttgt
    55261 gctatttcaa ttatactcaa tgaaaatcaa agagcaaact aggaagctag ccgcaggttg
    55321 ctcaaaatac tgttttgagg ttgcagatag agctgacgtg gtttgaagag attttcgaag
    55381 agtattagat tcgtgtagcg accatgagag atgctccagc ttggatcgtt gtcggataag
    55441 ttccagtaat agtcgctgta taagcatctt ggttggtgat gataacagga gtttctgtca
    55501 ccagacctgc agccttaatg acatccatat caaaacgaat cagttgctga ccaactgtaa
    55561 cgtgatctcc ttggactaca agactttcaa aacctttgcc atcaagacct actgtatcca
    55621 taccgatgtg gatgagcaat tcaactccct cgtcagagac aatgccgatg gcatgcttgg
    55681 tagggaaaag aaccgtcact gtcccattaa ctggagaggt caactcacct tggcttggtt
    55741 caatgaccag gccttgcccc atgacacctg atgcaaaaac aggatccgtc gcttgactca
    55801 attctttcac ttggccagtt agtgggctga taatttctac cgaagtaagt tctactggtt
    55861 catggctcac aaattctgct tcttcttgag caacgaattc tgcctgcaag ttcgtatcgc
    55921 cctctgtttt tgtgaagaga ccagccttgc agaagaagaa agtcaaaagc attggaacaa
    55981 caatcgcaac tagcatagtt cctgcaaatg gcagcatgta ttgaggttga atagagagaa
    56041 tacctggcaa accaccgata ccaatagaag ccgcagttac attaaaagta acggataaca
    56101 tgcctgcaag ggctgaacca gtcatcccag caacaaatgg ataaatatat tttacgttaa
    56161 ccccaaaaag agctggttct gtaacaccga gataggctga aatggttgca ggaagtgaaa
    56221 cctgagcctc acgctcatca tggcgatgca tgaaataata ggtaaagacg gctgagcctt
    56281 gagcaatatt agaaagagca atcataggcc agagggcagt gccaccagca tccgcaatca
    56341 attgtgtatc aatggcattg gtcatgtggt gcagacctgt gatgacaaat ggagcgtaga
    56401 gggcaccaaa aattgcaccg aagagccatt taactggacc agttaaacct gccaagacaa
    56461 ctgatgaaag tccttgtcca attgtccaac cgattggtcc caaaacagta tgagccaaaa
    56521 tcaaggctgg aatcaatgac aaaaaaggta caaaaatcat ggaaatgact tctgggatat
    56581 gtttgcgcca gaaaatttca agataagaca gactcaaacc tgcaagcaag gctgggataa
    56641 cttgggcttg gtaaccaata cgattaacag taaaataacc aaaattccaa acccagtttg
    56701 ctgcgatatc agctgctggc gttgaagcaa ccgcataggc attgagcaac tgaggcgata
    56761 ccaaacagat tccgagaaca attcccaaaa tttggctggt tcccatctta cgagaaacag
    56821 accaagtaat ccctactggt aagaactgga agatagcttc accaggcaac cagaggaagt
    56881 gattaacccc tgcccaaaac tgagaggatt ctgtgatggt cttgccatcc aacatcgacc
    56941 aatggacacc ttccaagaca ttacggaaac cgaggatcaa tccaccaaca atcaaggctg
    57001 gaataatcgg agtaaagatt tccgccagag tggtcataac accttggacc acgttttgat
    57061 tactcttagc tgcagacttg gctgcttctt tggaaacacc ctcaatacct gaaacggctg
    57121 taaaatcatt ataaaagatg ggcacgtcat ttccaatgat tacctgaaat tgacctgcat
    57181 ttgtaaaagt tcctttaaca gctggaattg actcgatagc tttaacatta gccttcttat
    57241 catctcctaa aacaaagcgc atccgtgtcg cacagtgagt tacggcagtc acattttctt
    57301 tgcctccaat tgcctgaagc agatctttgg cttcttgttc aaattttccc atttgattct
    57361 ccttatactt gtcaaagcaa gtttgatttg ataagtatat tgtaaacgat accaaacttg
    57421 tctgcaactt gttttgtata aaaagggtgt ttttttgtaa attttctagc acttatatgg
    57481 taaaatagat acaggtgttc attaaactag actaaaaacc tatttaagca ggcaaaatga
    57541 agaaatacca acaattattt aagcaaatcc aagaaaccat tcaaaacgag acttacgctg
    57601 tcggagattt ccttcctagc gagcacgacc ttatggagca atatcaagtg agtcgtgata
    57661 ccgtccgaaa ggccctgtct ctcctccaag aggaaggatt gatcaaaaag ataagagggc
    57721 aaggttctca agtcgtcaaa gaagaaaccg tcaatttccc tgtatccaac ctaaccagct
    57781 accaagaact agttaaagaa cttggactgc gctctaaaac caacgtggtc agtctggaca
    57841 agattattat tgataaaaaa tcctcactga taaccggttt cccagagttt cggatggttt
    57901 ggaaggtggt ccgccagcgt gtggtggatg atctggtatc cgttctggat acggactatc
    57961 tggatatgga actaattcca aatctcactc gccaaattgc tgagcagtct atctattctt
    58021 atatagaaaa tggcctcaaa ctccttattg attatgctca gaaggaaatc accattgacc
    58081 actcaagcga ccgagacaag attctcatgg acattggcaa agacccttat gtcgtttcga
    58141 ttaaatcaaa agtctatctc caagacggac gccaatttca gtttaccgaa agtcgccata
    58201 agttagagaa atttagattt gtagattttg caaaacgcaa gaaataaaag actgagacac
    58261 cagatctcag cctttttctt tatcccaaaa ctaattcatc actgaccaga ctttggccac
    58321 tatttttctg gaagagatgc ataaggtctt caactgtcag atgctttttc tcttctccct
    58381 tgacatctac cactatcttt ccttgataga gcataatgag acgattgccg tactcaatcg
    58441 catgattcat atcatgggta atcatcaaag tcgtccactg atggtgctcc acaatctttt
    58501 gcgtcaagtc catcaccatt tgactggttt tcgggtcaag tgccgcagta tgttcatcca
    58561 aaagcaaaag tttgggtttc accagagctg ccatgactag ggtcaaagcc tgtctttgtc
    58621 ctcctgagag atattgagta tctactttta agcggttttc aagaccaata ttcaactcct
    58681 tcaaggcctc ttggaactgg attctatcct tctccttcac gccccaacca agccctctct
    58741 tttgcccacg tctcaaggca atagccatat tctcctcaat cgtcaaacta gaagctgttc
    58801 ccatcttagg atcttgaaaa acacgggcaa tatccttggc tctctttcta acactcagat
    58861 ttttaatgga tttccctgcc agtagaaggt ccccttcgtc caccgataga ttgccagcca
    58921 agatattcat caaagtggat tttcctgctc catttccacc aatcacagag ataaaatctc
    58981 cctcttcaac ctctaaatct aatcctttga ggacatggtt ctcattgacc gtccctgctt
    59041 caaatgtctt gtgaatattt tcaagtgtta acaaacttgc cataactttc tcctcctatt
    59101 catttctcaa tttcagacca cgaatcttta atctcttttg caattctggt gcaaataaaa
    59161 ccaaagtgag caagattgca ttaaagagac gtaccaagtt ttgatctaga tttggaattt
    59221 catagatatt taaaataatc aaacggtaaa caatagcacc gattccgatg gataataagc
    59281 ggcctccaat ggtcaagtcg tgtatcaaga cttccgcaat aatcaccgag ctcaaaccaa
    59341 caacaatggt tcctgtccca gaagtcacat ctgaaaatcc atcattttgg gcaaacaagg
    59401 aaccacatag agcaatcaaa ccgtttgaaa tcatgtaacc aacaatcttc atggtgtcta
    59461 cattgactcc attggcctca ctcatcggga tattatcccc agtcgaacgc aagaccaagc
    59521 caatctctgt tttcatcaaa agggtcaaga ccaaacaaac aagtaaaaaa cagaccaaac
    59581 taagtgagaa aacagctcct tcatttgtca gacccaagct cgccaactgt ttaaagacgg
    59641 ttgcagaatc tcccaaggaa agattgggta cactgcccat gattttaata ttgattgaat
    59701 agagccctgt caaggtcaca atccctgtca agagagctgg gattttcatc ttggtgtgga
    59761 gcattcctga tacaagacct gctaccatac ctgccagcaa agcaagtaaa gtcgcaagcc
    59821 aaggatttgc ccctgcctgt atctgtgata caacgactgc tgcccccaga ggaaaggctc
    59881 cctcagcagt catatccgca atatccaaaa tacggaaagt caagtagtcc ccaatcgcca
    59941 caatcgacca caacaaacct tctgataaac tagataacac aaaattcatc ttaatcctcc
    60001 ttattccttg ccttctaact tactaatatc aattcccaat gcatctgcca tttcttgatt
    60061 ggtatgcaat tccagttttt caggcaactc aactgctata ttttctggtt cctcaccttt
    60121 taaaacacga atcagcatgc gagctgtctg tcttcccaat tcttcataat tggttccgta
    60181 gttatacaag ccaccaacag caatcatttc tgttgaacca ccgaatactg gaaccttgtg
    60241 tttaatagaa acctgcttaa ctgtttccat ggttgacaaa atgatattat cagttgggac
    60301 aaaaaccata tccacctctg ccatcaaact ctctgctgcc gccttgacgt tattactgtc
    60361 caagattgtt ttttcaacaa cggtaaagcc tttttctttc agaagtcgct tagcttcatc
    60421 cttttaaaca accgaattgg gctcgctctg agtatagagg attccaatcg ttttagcctt
    60481 tagcaacact ttctttatca aattgatttg ggttgaaatg gcatctgatg actgatcgct
    60541 tgtcccagtt acattgcccc caggatgttc tcttgactca accaacttgg cactgactgg
    60601 gtctgttaca gctgaaaaga taactggtgt cgtttgcgtc gtattggcca agctctgagc
    60661 agaaggcctt gcgatggcta gaacgacatc gctggattct gctagttgct gggaaatcgt
    60721 ttttagattt ctttgttctc cctgtgcatt ttgcaaatca atctcaatat tttccccctc
    60781 aacatagcct tgtttggcca gctcatccac aaatccttct ctagtagcgt ataaggactg
    60841 gtgggtaata aattgcgaaa taccgatacg aaagacatct ttttcttatc tgccttcaac
    60901 tgatgcaaac tgatcagaga agtcaagagg agccccacta ccaagagggg gctagtgatt
    60961 ttcgaacaac tttcataatg aaactccttc tcagaaaaga taaaacaaaa aaccgcctag
    61021 atactatcta aacggatact tgaaataacc taacaagtta caacttagct agtccattta
    61081 gatattcatc tatatggacc acaatcaaaa atcttagcca catagataca aacaaattgc
    61141 ttgaactgtt tgcatccatg gtgacatgtc tacaaacact atctaaagta gctaaagtaa
    61201 aagttttgat tcatagttac ggcttgtctc ttattcattt ctttttctgc tttctttttt
    61261 gatgcttcct atcctatcac ctttttcatc acctgtcaat aatatttcag aattttttaa
    61321 aatttttcga aaattctttc aggaagcttt caaagttttt caaatcagtc caacaaaaaa
    61381 agttttataa tttccataaa aattgacatg gaaattataa aaccattact agtttagtcc
    61441 tttttgataa cgtgccaatt cggcttggtt cgcccaaaca tagtgacctg gacggatttc
    61501 taccatagat ggcttatcag tctcatagtc gtgttgactt ggatcgtaaa ccttcaagac
    61561 cttcttacgt tccaaaattg gatctgggat tggtaccgct gaaagcaagg cttgagtata
    61621 tgggtgaatt ggattgttaa acaattcttc tgtttctgca acctctacaa taacaccctt
    61681 gtaaataact gcgatacgat ctgaaataaa gcgaacaacc gacaagtcat gggcgatgaa
    61741 gagataggtc aggccgagct ctttttggaa ttttttgagc aagttcaaga cttgggcacg
    61801 tacagaaacg tccaaggctg aaattggctc atctgcaata acaaagtctg gttgcatgac
    61861 caaggcacgg gcaataccga tacgttgacg ttgaccgcct gagaattcat gagggtaacg
    61921 agtcaagtgc tcagcaagaa gacctacttc acggataata ttttgaactt tctctttacg
    61981 ttcttcttca tccttaaata aacggtgatt gtaaagacct tcagaaataa tataatcaac
    62041 agtcgcacgt tcattcaaac ttgcggcagg gtcttggaaa atcatctgga ttcgacgaat
    62101 caattccgca gcttgttcac gcgatttctt accattaatc ttttgaccat caaaaatgat
    62161 atctccatta cttgtatcat ttagaccgat gatagcacga ccaatagttg ttttcccact
    62221 accggactca cctacaagcg agaaagtttc tcccttgttg ataaagaagt tagcattttt
    62281 aaccgcgaca aacttcttac ttccttcacc gaaggaaatt tctaaatctt tgatttctac
    62341 taatttttca gacatttcct tcctcctagt cagccagatg ggcaaatccc attttttcac
    62401 ggatcttatc atggagattt gcaatcacag ctggtttttc tactttcgga gcatcctcat
    62461 gaagaagcca agttttagcc caatgtgtct ctgatactga gaattgagga gctttttgtt
    62521 cgaagtcaat ctgcattgcg tagtcagaac gcaaggcaaa agcatcccct ttcaggtcag
    62581 tataaagtga cggaggtgtt cctgggattg agtaaagttc ccctttatca tcagcaagct
    62641 gaggcaagct agacaataga ctccatgtat atggatggcg agggtcatag aagacttcct
    62701 caaccgttcc atactcaacg atttctcctg catacataac cgctacctta tccgcaatac
    62761 ttgccaccac accaaggtcg tgggtaataa agattgttgt gaaatgatac tcgttttgta
    62821 aagattttag caaatcaata atctgagctt gaatagttac atccaaggca gttgttggct
    62881 catcacagat caagacatca ggtcggcagg caagggcaat cgcaataacg atacgttgac
    62941 gcattcctcc agaatattgg aatgggtatt cattaaaacg tctatctgcg tctggaatgc
    63001 caaccttatt catgtagtca atggccaatt ctttcgcttc tttagctgtt tttccttggt
    63061 gttttacaat aacttctgta atctgactac caattgtttt aatggggtcc aaactagtca
    63121 ttgggtcctg gaagatagtc gcaatcttag caccacgaat ttgttcccaa tccttgtgag
    63181 aagataaagc tgtcaagtcc tgaccacggt agtcaatact accttgggca atacgaccat
    63241 tttcttcgag catacctgtg aaggtctttg tcaaaacaga tttacctgat cctgactcac
    63301 ctaccaaggc taatacttct ccttcgacta gttcaaggga aacgccgcga atggctgtca
    63361 atactttgtc acgaacgtca aattccacga caatatcgcg agcagtcaaa attacatttt
    63421 tttcttttgt catttctact cctatctatg tgtacgtgga tcactagcat ccgctaagtt
    63481 ttgaccaact acgaaaaggg acaaggatac caagacaagg gttgtcaatg gaatccagaa
    63541 caagtaagca ttggttgtta cgttttgtga ataatccgaa atcaaacgac ccaaacttgg
    63601 cactgtaatc ggtaatccaa gaccgaagaa agacaagaag gcttcgtatg agataaagct
    63661 tggaagcatt tgagtcatgg ttgtcacaat aacagatacc aattgaggca tgatattttt
    63721 ggcaacaatc ttcaaggttg gtgttcccaa agtacgtgac gccaagttgt attccaagtc
    63781 acgatagcgc aagatttgca cacggatcat gaaggcaata ccaatccatg ttgttacgct
    63841 catggcaaaa atcagattcc agaatccagc tccgattgag taagtcaaga caataacaat
    63901 caaaagaggt gggatgtttg agatgacgtt gtaaacttcc atcatgacac ggtcaactga
    63961 ttttgaaata ccccaaatac caccgacaaa aacaccgata accaagttaa tcactgtcgc
    64021 aatcacagaa atgaggatgg agttacgagc tccgaaccag acaccgtcaa agagcgattt
    64081 accgttactg tcagtaccga accaatgctc cgcatttggc ttgatataac gaacactaaa
    64141 gtcgtttacc ttgctgacat cattgaaatc aaacttagaa aacattgggt agatgaaact
    64201 tatcaaaatg atggctacca agattcccaa catgactaca gttgattttt tcttcataaa
    64261 ttgtttaaac actgatttcc agtaagaata tgctggcgca tcaatagttt cagaggcaaa
    64321 atcgtcacgt tttacaaact gaaatttttc tttatcgatt gtagacatta tttgcctcct
    64381 ttctcagtca atttaatacg tgggtcaata atagtcatcc aaatatctcc caaaagacgt
    64441 gagaagatag aaatacatgt aaagatgaag acaagaccaa cgaccataga gttattagat
    64501 gcttttacag agtcaatcaa cattttaccc atacctggga aggcgaagac tgtttcagta
    64561 agggttgcac caccgataac cccaataatg gcaccaggaa ttcctgaaac cagcggaacc
    64621 atggcatttt taaagatgtg tttgtttgaa atttcttttt cagacaaacc ttttgcacga
    64681 gcgaaacgaa caaagtcttg agattgcaag tcaatcatgt aacgacgaat ccaaatggct
    64741 gtaacaggag cacccaacaa accaaggatg actgctggta aaacgtaaga acgccaatct
    64801 ccagctccca agatagggaa tgaatctgga agggcaatag atgatccaat caatcgaacg
    64861 atgtaaacca aggcaatcgt tggaagagca agcaagaagg tcaaagtccc tgttgagagg
    64921 ctatcaatcc aagtgttctt gaaacgagcc atggctgaac caagtggcac ggcaagagca
    64981 taggcaagaa ccaaaccaat caaaccagta atagcagagc tgacaatcat agatggatat
    65041 tggtaattac tttcagtcgc tgtataagga tcatctttcc catagctagc tacttcacga
    65101 gagtcagcct gactaggtga cttgtaggtt cttgagtaaa tatttacaga agacgttttc
    65161 ttacctgttg ggaactgaac ttgggcagtt ttggtttgtc cttgaccttg agtaataacc
    65221 tgaagaactg gtgtattagc ataggttggg taagagtcac ctaaattcaa gttcacaaag
    65281 ttttgatgaa caaatgggaa ctgactgtta aagtacaaga gatatttatg tttagttcct
    65341 gaaccgacca atgaccatcc gatagctgga tcattttcaa aacgaaggta gcgtttcaag
    65401 tctggatttt cagggtcttg gattttattt gtatgatcaa tgtcaatcaa gttagcatag
    65461 aagtgaaaaa cacgttcaaa aattggaatt tcacgagtag catagaattg accactttca
    65521 gtaaattctc ccaaagtcca accatgacct aattgattga tgtacttttc ataaatagct
    65581 ttattggtcg catttgcttc tactgttaca gaagaatcca tgctacttgc cttttcttgc
    65641 aactctttag tatcgtaata ctcaatgtag cccatacgct caaacacagt attttcatag
    65701 ttatcacgtt tatcagccgt tgtcgcaatt ttattatagt taggatcctg cttgaaaatc
    65761 aattttcgag gaaccaaggt atagataatc gtgtaggtca aagtcgttac taagaaaatc
    65821 gaaaccaatg accgcaaaac acgcataaaa atatattttt tcatattatt tcctttaaaa
    65881 atcccaaaag aaccttctcc tcatggagag aaagttctat tagaaattat ttacttcaca
    65941 tgacttgcca attctttttg agctttctca tttgattcag ctttttcttt caaccatttt
    66001 tcacgagctt tttcatactc ttccttagtc accactttat cttgtaattt caaatatttg
    66061 aagtaaacat ctgacccctt agagcctgtt tgcgcagaag ctccagtaaa tggaacaatt
    66121 cgtgaaagca ctggtgctgc accagaagaa gccatagcag gaataaagag tgaactatct
    66181 gtcaaccatg cttgagccgc tgcatatttt tcataacgga cattcaagtc gcttgtctct
    66241 ctggcagctt catcaactaa tttatcgtat tctttcaaac caacttgaac tactgaaggg
    66301 ctatttggat tatcaaatcc taaatatgtt tttgtagttt cactgctagt tgtttttaaa
    66361 atatccaggt aagtagatgg gtcttgatag tctggccccc atgaaactcc tcctgataca
    66421 tcccaatcct cagatgaagc attggcagca tagtaagtaa tattaaggaa ttcatcactt
    66481 gtcatttgtt gaatatcaac aacgacattt tcaacaccaa gaactgtttc tacagattgt
    66541 ttaaaggact gaatacgaga tatgtagttt tttgatgctt ggtctactgg aacgtccaga
    66601 tgaataggaa actgaacgcc gtctgcttct aaagctttct tagctttcgc aaactctgcc
    66661 ttggccttgt cagcattgaa taaaccatcc tgcccatcag ctaaattcac acctttccac
    66721 tcatcaccat aagcaggaag ttgagcagcg actaaatcac caaaggtctt ctcaccagct
    66781 gaaacaaagt ctggttttac aaataaatta cgaactgcta aagctgctcc atctttacca
    66841 ttgatttgag ctgagtaagc tgagcgatca agagcaaaat tcaaggcttg acggaaatct
    66901 ttgttaagca atgccttctt agtagctact ttctctgaat ctgtagtttt agaagtatag
    66961 ttgtaacttt ggcgatcaat attcacaccc agaccagcaa tcccagagcc tgattgtgtg
    67021 taatagatat tgtccttgta ttcttctgca accttagaat agttggagct ggtagggtaa
    67081 agacgggcat aactataagc tccactagtg aagttacgct ctagcgactc ctgatctgat
    67141 ccatcatagt aagctagatt gatagtatct aggtggacat tttctttatc ccaatattgc
    67201 tcatttttta caaactctac agaagatttt gcagtcaacc ctttcaacaa gaatggacca
    67261 ttataaagca aggatgtcgg atctgttggt ttagcaaaat cgcttccttt tgatgtttcg
    67321 aattcttcat tcagaggcca gaaaatagaa taggtcaact tagagttcca gaacggttca
    67381 ggctggttca aagtgtattg taacgtataa tcatcaaccg ccttgacacc aactgttgaa
    67441 aaatctgttg aagttcctga tagataatct gccaagcctt taaccgaatt ttcagctaaa
    67501 tacatagctt ctgatttttt atctgctgcg tgttttaaac cgttcacgaa atctttagcc
    67561 gtcacctctg catattcttc tccatcagag gtaaaccatt taaccccttt acgaatctta
    67621 taagtgtagg tcaaaccatc cttagagact tcccaatcct ctgcaactgc aggagcaaga
    67681 ttaccgtaat tatcgttagt gaataaacca tcaatcccat ttgaagtcac tactgttgta
    67741 ctatttttac ttgaaatcag gtagtccaag gtttctgggt ctgctgtata aacatagcca
    67801 taagctttag gggctgatga atcagatgat tttgaagaac tgcatgctgc aagtacacct
    67861 gctgctaata aaacaagacc tgctgtagca aatacacgat tttttttcat tttctactcc
    67921 tctgtttatg tgaattatag attgacaacc attatatcac attatccatt aaaaatcaaa
    67981 caaattttca gaatatttag gcttgttggc acaaattttt catttttttt gaatatatga
    68041 ttcaaattgt cgttcgaagt gtcaaagact acagtgaaaa taggaaattt gacgcagaaa
    68101 ctttggagtt taggaagaca tacagtaaaa tgaaatacgg acggaacaat gtgattttgg
    68161 aattcaaatt aaattataac aatattgtag aagtatcatt ctagtattca agattcagtt
    68221 tactatgtct tttcacacca accttattcc gaattcaatt acttttgtga tttacatata
    68281 tagattaaga ctatctttta tactttaaaa tttctcgcta ccttatccac tatatgctcc
    68341 tcgctatcac gtttctattc atagcctacg atttcactat tgctttctct gacaattctt
    68401 atttcctgcg tcagacttaa aacgatctat ccccagacca ttttaatccg ctacctcacg
    68461 atagtcaggc ttggggagcg ctattgtatt caccggtagt ggatccctac agaggactta
    68521 cacctcagat gcacgacatg cccatcgtat aaaaaaatct cctacccaag gtagaagatt
//
LOCUS       DAVDWT010000008        61650 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVDWT010000008 DAVDWT010000000
VERSION     DAVDWT010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 61650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.008"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            342..1385
                     /locus_tag="VUW50_000610"
     CDS             342..1385
                     /locus_tag="VUW50_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HET7982560.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEHFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            1552..3639
                     /gene="topA"
                     /locus_tag="VUW50_000611"
     CDS             1552..3639
                     /gene="topA"
                     /locus_tag="VUW50_000611"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET7982561.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3860..4219
                     /locus_tag="VUW50_000612"
     CDS             3860..4219
                     /locus_tag="VUW50_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HET7982562.1"
                     /translation="MRIIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFASLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4312..4944
                     /locus_tag="VUW50_000613"
     CDS             4312..4944
                     /locus_tag="VUW50_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HET7982563.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            4988..5644
                     /locus_tag="VUW50_000614"
     CDS             4988..5644
                     /locus_tag="VUW50_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET7982564.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5663..6284
                     /locus_tag="VUW50_000615"
                     /pseudo
     CDS             5663..6284
                     /locus_tag="VUW50_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
     gene            6312..6746
                     /locus_tag="VUW50_000616"
     CDS             6312..6746
                     /locus_tag="VUW50_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HET7982565.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTIDAVTDGIDTQDRVLVTVENRDTETIFNA
                     AGLDFDVFKASAIAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6758..7795
                     /gene="leuB"
                     /locus_tag="VUW50_000617"
     CDS             6758..7795
                     /gene="leuB"
                     /locus_tag="VUW50_000617"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET7982566.1"
                     /translation="MTKKIVALAGDGIGPEIMEAGLEVLEALAKKTGFVYEIDRRPFG
                     GAGIDAAGHPLPDETLKACREADAILLAAIGSPQYDGAVVRPEQGLLALRKELNLYAN
                     IRPVKIFESLKHLSPLKSERIAGVDFVIVRELTGGIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPTSMILSVAMMLRDSFGRYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            7792..8061
                     /locus_tag="VUW50_000618"
     CDS             7792..8061
                     /locus_tag="VUW50_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HET7982567.1"
                     /translation="MKLDEKIALVLLIWNVIIFLIYGIDKFKARRRAWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            8065..8424
                     /gene="leuD"
                     /locus_tag="VUW50_000619"
     CDS             8065..8424
                     /gene="leuD"
                     /locus_tag="VUW50_000619"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET7982568.1"
                     /translation="MAGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNTLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8586..9210
                     /locus_tag="VUW50_000620"
                     /pseudo
     CDS             8586..9210
                     /locus_tag="VUW50_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            9375..11306
                     /locus_tag="VUW50_000621"
     CDS             9375..11306
                     /locus_tag="VUW50_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HET7982569.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGSSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYSEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPDSNEDGSIFFKLKEGIFKKFCQNAID
                     SKKIGGQDNFDEARDLYLEYVNNIDEKEYLTEFSYLTVNSRNNFNINYETKAKGTVLT
                     KSYVYELYKDENYLKQPYYHNQGKKVLETLKKRFGLKDYISPEEVETDKNFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTVEGQAGMLDKELNIHAEEAKIRLRNLNAAIENIQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFDLTNNEQ
                     KDQAVADDNEGDENDDADY"
     gene            11293..12609
                     /locus_tag="VUW50_000622"
     CDS             11293..12609
                     /locus_tag="VUW50_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HET7982570.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNPLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRSHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGSQLKKWSIRIPQNASFYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            12784..13770
                     /locus_tag="VUW50_000623"
     CDS             12784..13770
                     /locus_tag="VUW50_000623"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HET7982571.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEVGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVSQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKVARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            13834..13905
                     /locus_tag="VUW50_000624"
     tRNA            13834..13905
                     /locus_tag="VUW50_000624"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13867..13869,aa:Arg,seq:ccg)
     gene            14088..14786
                     /locus_tag="VUW50_000625"
     CDS             14088..14786
                     /locus_tag="VUW50_000625"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET7982572.1"
                     /translation="MKELQTVLKNHFAIEFTDKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            14777..18316
                     /gene="smc"
                     /locus_tag="VUW50_000626"
     CDS             14777..18316
                     /gene="smc"
                     /locus_tag="VUW50_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET7982573.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLATLEDKRSS
                     LSQEKSDKESSLALLEENLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLATAKEKAGQQKAELETAKEQVQK
                     LLADYQVCAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLEIILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVHYQTALEIALGASSQHIIVEDEESATKAIDLLKR
                     NRSGRATFLPLTTIKARTISSQNQDTIAASPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLRSDEVSLKNLQDELARLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEELNRLSEGEWQADKEKCQERLTTITSEKNNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDIEQEEIQRLLQEKVDN
                     LEKVDTDLLSQQADEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNLDAIEQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEVIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            18313..19107
                     /locus_tag="VUW50_000627"
     CDS             18313..19107
                     /locus_tag="VUW50_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET7982574.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLVSYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEDGEAWVNENVPGVKAMTTGFESIDIVLDYVDKGVAIVELVKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            19107..19925
                     /locus_tag="VUW50_000628"
     CDS             19107..19925
                     /locus_tag="VUW50_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET7982575.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLQAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            19929..21218
                     /gene="ftsY"
                     /locus_tag="VUW50_000629"
     CDS             19929..21218
                     /gene="ftsY"
                     /locus_tag="VUW50_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET7982576.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESQDSEPAVGVSQEEIEELPNSEELPEEEKLEHE
                     GTVEENNSEVLEPEAPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDVLRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKQAGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVTEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(21264..22751)
                     /locus_tag="VUW50_000630"
     CDS             complement(21264..22751)
                     /locus_tag="VUW50_000630"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET7982577.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(22900..23640)
                     /locus_tag="VUW50_000631"
     CDS             complement(22900..23640)
                     /locus_tag="VUW50_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET7982578.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(23640..25805)
                     /locus_tag="VUW50_000632"
     CDS             complement(23640..25805)
                     /locus_tag="VUW50_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET7982579.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYTILAIFYLVIITLLTRLAKRLEKRIR"
     gene            25975..26928
                     /locus_tag="VUW50_000633"
     CDS             25975..26928
                     /locus_tag="VUW50_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7982580.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTFLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            27005..28993
                     /gene="uvrB"
                     /locus_tag="VUW50_000634"
     CDS             27005..28993
                     /gene="uvrB"
                     /locus_tag="VUW50_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HET7982581.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAVAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            29003..29566
                     /locus_tag="VUW50_000635"
     CDS             29003..29566
                     /locus_tag="VUW50_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7982582.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            29532..30020
                     /locus_tag="VUW50_000636"
     CDS             29532..30020
                     /locus_tag="VUW50_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7982583.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWRESYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            30094..30561
                     /locus_tag="VUW50_000637"
     CDS             30094..30561
                     /locus_tag="VUW50_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET7982584.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(30558..31073)
                     /locus_tag="VUW50_000638"
     CDS             complement(30558..31073)
                     /locus_tag="VUW50_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HET7982585.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNLYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(31085..31612)
                     /locus_tag="VUW50_000639"
     CDS             complement(31085..31612)
                     /locus_tag="VUW50_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET7982586.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(31894..32457)
                     /locus_tag="VUW50_000640"
     CDS             complement(31894..32457)
                     /locus_tag="VUW50_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET7982587.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(32441..32992)
                     /gene="coaC"
                     /locus_tag="VUW50_000641"
     CDS             complement(32441..32992)
                     /gene="coaC"
                     /locus_tag="VUW50_000641"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HET7982588.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(33004..33693)
                     /gene="coaB"
                     /locus_tag="VUW50_000642"
     CDS             complement(33004..33693)
                     /gene="coaB"
                     /locus_tag="VUW50_000642"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HET7982589.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            33923..35593
                     /locus_tag="VUW50_000643"
     CDS             33923..35593
                     /locus_tag="VUW50_000643"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET7982590.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKSQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            35921..37096
                     /gene="mutY"
                     /locus_tag="VUW50_000644"
     CDS             35921..37096
                     /gene="mutY"
                     /locus_tag="VUW50_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET7982591.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            37151..37855
                     /locus_tag="VUW50_000645"
     CDS             37151..37855
                     /locus_tag="VUW50_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7982592.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            37848..39197
                     /gene="vicK"
                     /locus_tag="VUW50_000646"
     CDS             37848..39197
                     /gene="vicK"
                     /locus_tag="VUW50_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HET7982593.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKILDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            39199..40008
                     /locus_tag="VUW50_000647"
     CDS             39199..40008
                     /locus_tag="VUW50_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7982594.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            40099..40341
                     /locus_tag="VUW50_000648"
     CDS             40099..40341
                     /locus_tag="VUW50_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HET7982595.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            40343..40597
                     /locus_tag="VUW50_000649"
     CDS             40343..40597
                     /locus_tag="VUW50_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET7982596.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            40644..41296
                     /locus_tag="VUW50_000650"
                     /pseudo
     CDS             40644..41296
                     /locus_tag="VUW50_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
     gene            complement(41556..42542)
                     /locus_tag="VUW50_000651"
     CDS             complement(41556..42542)
                     /locus_tag="VUW50_000651"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HET7982597.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            42736..45204
                     /gene="gyrA"
                     /locus_tag="VUW50_000652"
     CDS             42736..45204
                     /gene="gyrA"
                     /locus_tag="VUW50_000652"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET7982598.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            45204..45947
                     /gene="srtA"
                     /locus_tag="VUW50_000653"
     CDS             45204..45947
                     /gene="srtA"
                     /locus_tag="VUW50_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HET7982599.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
     gene            46191..46988
                     /locus_tag="VUW50_000654"
     CDS             46191..46988
                     /locus_tag="VUW50_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HET7982600.1"
                     /translation="MISSEFISKIEFACNKKESLYSQSKFKYAIRSIFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGSLIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            47136..47258
                     /locus_tag="VUW50_000655"
     CDS             47136..47258
                     /locus_tag="VUW50_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET7982601.1"
                     /translation="MTRDFKFETLQLHAGQVVTPATESRAVPIYQTTSFVFDDT"
     gene            47280..48320
                     /locus_tag="VUW50_000656"
     CDS             47280..48320
                     /locus_tag="VUW50_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HET7982602.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILAIAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFFDIDNLEEVEAAIKDNTKLVLIETLG
                     NPLINIPDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDIAIHSVTKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTRDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKGVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            48491..49765
                     /locus_tag="VUW50_000657"
     CDS             48491..49765
                     /locus_tag="VUW50_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HET7982603.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            49775..50653
                     /gene="truB"
                     /locus_tag="VUW50_000658"
     CDS             49775..50653
                     /gene="truB"
                     /locus_tag="VUW50_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET7982604.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTKTEDASGEVVAETPVLSLLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLSPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            50894..51196
                     /locus_tag="VUW50_000659"
     CDS             50894..51196
                     /locus_tag="VUW50_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET7982605.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQILAQTLQGFR"
     gene            51304..51942
                     /gene="udk"
                     /locus_tag="VUW50_000660"
     CDS             51304..51942
                     /gene="udk"
                     /locus_tag="VUW50_000660"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET7982606.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            52070..53410
                     /locus_tag="VUW50_000661"
     CDS             52070..53410
                     /locus_tag="VUW50_000661"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET7982607.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQVTIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQWDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            53388..53600
                     /locus_tag="VUW50_000662"
     CDS             53388..53600
                     /locus_tag="VUW50_000662"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET7982608.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            53597..54472
                     /locus_tag="VUW50_000663"
     CDS             53597..54472
                     /locus_tag="VUW50_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET7982609.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            54465..55280
                     /locus_tag="VUW50_000664"
     CDS             54465..55280
                     /locus_tag="VUW50_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET7982610.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDVKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            55273..55704
                     /locus_tag="VUW50_000665"
     CDS             55273..55704
                     /locus_tag="VUW50_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET7982611.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            55711..57378
                     /gene="recN"
                     /locus_tag="VUW50_000666"
     CDS             55711..57378
                     /gene="recN"
                     /locus_tag="VUW50_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET7982612.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGVPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLHLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            57380..58108
                     /locus_tag="VUW50_000667"
     CDS             57380..58108
                     /locus_tag="VUW50_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HET7982613.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQAQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            58187..60010
                     /gene="lepA"
                     /locus_tag="VUW50_000668"
     CDS             58187..60010
                     /gene="lepA"
                     /locus_tag="VUW50_000668"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET7982614.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(60169..60249)
                     /locus_tag="VUW50_000669"
                     /pseudo
     CDS             complement(60169..60249)
                     /locus_tag="VUW50_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            60423..60899
                     /locus_tag="VUW50_000670"
     CDS             60423..60899
                     /locus_tag="VUW50_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7982615.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVASLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIASDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            60937..61239
                     /locus_tag="VUW50_000671"
     CDS             60937..61239
                     /locus_tag="VUW50_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET7982616.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFCIPAMSVPVFDNIILPAKQNMV"
     gene            61247..61650
                     /locus_tag="VUW50_000672"
                     /pseudo
     CDS             61247..61650
                     /locus_tag="VUW50_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT        18880 a        10932 c        13880 g        17958 t
ORIGIN      
        1 tagagattac aaggcttgtt taagaaagaa ttcaaagacc ttgacaaata aaaataaaat
       61 ggttattata aaaaatggtc tgaaatagat gatgatactt ttcgaaaatc tttttacgtc
      121 agctcagctt tgccttgctg tgttttgagc aagctacggt tagcttccga gtttgatttt
      181 catttactag aaatgaaact gatgagagat atcagtagac atttgagtca ggatattatg
      241 gaaaatgata aaaagagctc gtgagattgg catatcagac tactaaagta ttgagtttgt
      301 taggatttta gcgactagtt agctgggaaa ggaagatatt tgtgacaaat aataaactgt
      361 attcgttgat agaatttaga aataaaatat atgaagaatt agaactttcc agaagtgatt
      421 tagcgatttt actatgtgcc atgcttatcg cctctatcgg attaaatatg gattcgactc
      481 ccgtgattat tggagccatg ttaatctctc ctttgatgac acctattctg ggagtggggc
      541 tctctctagc tatatttgat tttaaattgt taagaaaatc ttttaaaata ttagctattc
      601 aaattcttgc cagtttaata gcttcaacac tttattttta tctttctccc atttcgtatg
      661 ctagttcgga aattgttgct agaacctctc cgactatttg ggatgttctc attgcttttg
      721 taggagggat agcaggtatt attggtgcta ggaaaaaaga gaccaataat attgttcctg
      781 gtgttgctat tgcaaccgcc ttgatgcctc ctctttgtac agtaggttat gctattgctt
      841 ctgctaatct aaaatttatc ataggctcct cttacctatt cctcatcaat tgtagcttta
      901 ttgtcattgc gacttatata ggtgttaggt tgatgatggt taagaaacat tattttaaag
      961 ataatgaaga agactctaaa atgcgtagga ttttgcttct agttgctgtt ttgctgatga
     1021 ttccgagttt catctctgca acgactttag tgagagaaac gttgaaaaaa gagtccctta
     1081 agaaatttat atcagagcag tttcaggggt ataatatttt gaaaaaaacc tattctaaaa
     1141 agactcatac cctaaagcta accatttcag gaaattattt gacagaagaa gaactcgata
     1201 tgatttcaag taagagaggt gactatggtt taagtgatgt ttctgttcaa gtttcacaat
     1261 tgtctgattc agaacaactt agtaaggaag agctggtgga gcatttcttc cagtatatca
     1321 aggataagga agcaaaagaa aaggagaaag ccaataaatt tgatacagag tctgaggagc
     1381 aataatttct tgagaatagc tggtttctct cgtgagtctt ctatgtatat caaaggaaga
     1441 ctgaggtttt aagtataaaa cttttcttct attatagtag ctaagtcttg acagggttca
     1501 aaaaatggtt tacactttat aaagtttatt actttgaaaa ggtgtgatac tgtggctacg
     1561 gcaacaaaaa agaaaaaatc aacagttaaa aaaaatctag tcatcgtgga gtcgcctgct
     1621 aaggccaaga cgattgaaaa atatctaggc agaaactaca aggttttagc cagtgtcggg
     1681 catatccgtg atttgaagaa atccagtatg tccgtcgata ttgaaaataa ttatgaaccg
     1741 caatatatta atatccgagg aaaaggccct cttatcaatg acttgaaaaa agaagctaaa
     1801 aaagctaata aagtttttct cgcgagtgac ccggaccgtg aaggagaagc gatttcttgg
     1861 catttggccc atattctcaa cttggatgaa aatgatgcca accgtgtggt cttcaatgaa
     1921 atcaccaagg atgcagtcaa aaatgctttt aaagaacctc gtaagatcga tatggacttg
     1981 gtcgatgccc aacaagctcg tcggatcttg gaccgcttgg tagggtattc gatttcgcct
     2041 attttgtgga agaaggtcaa gaagggcttg tcagcaggtc gcgttcagtc cattgccctt
     2101 aaactcatca ttgaccgtga aaatgaaatc aatgccttcc agccagaaga atactggaca
     2161 gttgatgctg tctttaaaaa gggaaccaaa caatttcatg cttccttcta tggagtagat
     2221 ggtaaaaaga tgaaactgac cagcaatgac gaggtcaagg aagtcttgtc tcgtctgact
     2281 agtaaagact tttcagtaga tcaggtggat aagaaagagc gcaagcgcaa tgctccttta
     2341 ccctatacca cttcatctat gcagatggat gctgccaata aaatcaattt ccgtactcga
     2401 aaaaccatga tggttgccca acagctctat gaaggaatta atattggttc tggtgttcaa
     2461 ggtttgatta cctatatgcg taccgattcg actcgtatca gtcctgtagc gcaaaatgag
     2521 gcggcaagct tcattacgga tcgttttggt agtaagtatt ctaagcacgg tagcaaggtc
     2581 aaaaacgcgt caggtgctca ggatgcccat gaggctattc gtccgtcaag tgtctttaat
     2641 acaccagaaa gcatcgctaa gtatctggac aaggatcagc tcaagctata tacccttatc
     2701 tggaatcgtt ttgtggctag ccagatgaca gcggccgttt ttgataccat ggctgttaaa
     2761 ttgtctcaaa aaggggttca atttgctgcc aatggtagtc aggttaagtt tgatggttat
     2821 cttgccattt ataatgattc tgacaagaat aagatgttac cggacatggt tgttggagat
     2881 gtggtcaaac aggtcaatag caaaccagag caacatttca cccaaccgcc tgcccgttat
     2941 tctgaagcaa cactgattaa aaccttagag gaaaatgggg ttggacgtcc atcaacctac
     3001 gcgccaacca ttgaaaccat tcagaaacgt tattatgttc gcctggcagc caaacgtttt
     3061 gaaccgacag agttgggaga aattgtcaat aagctcatcg ttgaatattt cccagatatc
     3121 gtaaacgtga ccttcacagc tgaaatggaa ggtaaactgg atgatgtcga agttggaaaa
     3181 gagcagtggc gacgggtcat tgatgccttt tacaaaccat tctctaaaga agttgccaag
     3241 gctgaagaag aaatggaaaa aatccagatt aaggatgaac cagctggatt tgactgtgaa
     3301 gtgtgtggca gtccaatggt cattaaactt ggtcgttttg gtaaattcta cgcttgtagc
     3361 aatttcccag attgccgtca tacccaagca atcgtgaaag agattggtgt tgagtgtcca
     3421 agctgtcatc agggacaaat tattgagcga aaaaccaagc gtaatcgcct attctatggt
     3481 tgcaatcgct atccagaatg tgaatttacc tcttgggaca agcctgttgg tcgtgactgt
     3541 ccaaaatgtg gcaacttcct catggagaaa aaagtccgtg gtggtggcaa gcaggttgtt
     3601 tgtagcaaag gcgactacga ggaagaaaag attaaataag aggaggggcc cgaaagtaaa
     3661 tttcaggctc cttttggtta gaacttgaca aaatacatca ttttatgcga aactagaaga
     3721 agattatttt atactcaatg agaatcaaag agcaaattag aaagctagtc gcaggttgct
     3781 caaagcacta ctttgaggtt gaagatggaa gctgacctgg tttgaagaga ttttcgaaga
     3841 gtattaacta ggagaagtta tgcgtattat ctatctaatt attggttttt tatcgctggc
     3901 cttggctatt gttggggttg ttttaccctt gttgcctaca acacctttcc ttttgttgtc
     3961 tattgcttgt ttctccagaa gttccaagcg attcgaagac tggctttatc ataccaagct
     4021 ctatcaaaca tatgtagctg attttcgcga gactaagtct attacgcgtg aacgaaagaa
     4081 aaaaatcatc gtctctatct acgtcttgat gggaatttct atttattttg catctctttt
     4141 accagtcaaa atcggtctgg gtgctttgac catctttatc acttattatc ttttcaaggt
     4201 cattccagac aaagaatagt taaaacagta gttatttgcc ttgataaaat tgaaagcata
     4261 ttcataacaa tatgatataa taaatttgaa gtaatattca aggagaatca aatgatttac
     4321 gaattttgtg ctgaaaatgt gactttactt gaaaaagcga tgcaggctgg agctcgtcgg
     4381 attgaactct gtgataatct agcagttggt gggacaacac ccagctatgg agtgactaag
     4441 gcagcggttg aactggcagc taactacgat acaaccatca tgaccatgat tcggccacgt
     4501 ggtggtgact ttgtctataa tgacctagaa attgctatca tgctagaaga cattcgtttg
     4561 actgctcagg ctggaagtca aggggttgta tttggagctt taactgctga taaaaagttg
     4621 gataagccta atctggaaaa gttaattgct gcatcaaaag gaatggaaat tgtctttcac
     4681 atggcctttg atgaactaag tgatgaagat caaccggaag ctattgactg gctcagtcaa
     4741 gccggtgtca ctcgtatcct aactcgtgct ggtgtgtctg gcgactcctt agaaaaacgt
     4801 tttgttcact atcacagaat tttggagtac gctaaaggta aaattgaaat tctaccaggt
     4861 ggggggattg accttgaaaa ccgtcaaacc tttatcgacc aggtgggggt aacacaattg
     4921 catggtacta aggttgtttt ttaaaaaata gaaaggaact gctagctttg ggtagcagtt
     4981 ttcacttatg tttgaaattt ttaaatccta tcaatttaat caagaaaagg ctcatgatta
     5041 tggttttata gaaaatagcg aagtctggac atatagttgc cagattttgc aaggtgactt
     5101 tgtcatgact gtgtccatca ctgctgataa tgtgaacttt caagtctttg accaagagac
     5161 tggtgacctc tatcctcacg ttcatatgga aagcatgagg ggaagttttg tcggaaatgt
     5221 ccgtgaggct tgtctggaga ttctttacca gattcggaag gcttgttttg atgtgcaaga
     5281 ttttatctgt catcagacta agcgtatcat gactcaagtt caggaaaagt atggaaacca
     5341 gttggagtat ctgtgggaaa aatcgcctga tacagctgta ttgcgccatg aaggcaatca
     5401 aaagtggtat gccgtcttga tgaaaatctc ttggaataag ctggaaaagg gcagagaagg
     5461 acaagtggaa gcagtcaacc tcaagcatga ccaagtagct aatttacttt cacaaaaggg
     5521 gatttatcca gcctttcata tgagcaagcg ctactggatt agtgtgtccc ttgatgatac
     5581 tttatcagat gaagaagtac tggaattgat agaaaaaagt tggaacttaa cctctaaaaa
     5641 atgaaatatt ttaataattt tcatgaactt tcaattggct aaatattctt tactgaagaa
     5701 attttcagaa aatataggat ttaccacact agaggaatat ggtgccatct tcaaatacct
     5761 gattgagaat gtcaagacgg atcgtcagat catctattcg cctcactgtc atgatgacct
     5821 cggaatggca gtggcaaata gccttgctgc tgtcaagaat ggtgcaggac gtgttgaagg
     5881 gactatcaat ggtattaggg agcgagctgg aaatgctgct ttggaagaaa ttgcagtggc
     5941 tctcaatatt cgccaagatt actaccaagt agaaaccagt attgtcctaa atgagaccat
     6001 caatacgtca gaaatggttt ctcgcttctc tggtattcca gttcctaaaa acaaagccgt
     6061 cgttggtggc aataccttct cccacgaatc tggtattcac caagatggag tccttaaaaa
     6121 tcctctcact tatgagatca tcacacctga attggttggt gttaagattc cgcttgggtc
     6181 gccatgcttt tgttgagaaa ctgagagaat tggccctaga ttttacagaa gaggatatca
     6241 aaccactctt tgctaagttc aaggcactgg ccgataagaa gtaagaaatc acagatgcag
     6301 atattcgagc tttggtagct ggaaccatgg ttgaaaatcc agaaggcttc cactttgatg
     6361 atttacaact tcaaactcat gcagataatg acattgaagc gctcgttagc ctagccaata
     6421 tggatggtga gaaagtcgaa tttaatgcga cagggcaagg ttccgttgaa gcaatcttta
     6481 atgctatcga taagttcttt aaccaatctg ttcgtttggt gtcctacact atcgatgcgg
     6541 taacagatgg aatcgatacc caggatcggg ttttggtcac tgttgaaaac agagatacag
     6601 aaaccatctt taatgcagca gggcttgatt ttgatgtgtt taaggcttct gctattgcct
     6661 atataaacgc taataccttt gttcaaaaag agaatgcagg tgagatggga cgcagtgttt
     6721 cttaccacga tatgcctagt gtgtaaagga gaaggctatg acaaagaaaa tagtagctct
     6781 agcaggagat ggaattggcc cagaaatcat ggaggctggt ttagaagttc tggaggctct
     6841 agctaaaaaa acaggctttg tctatgaaat agacagacga ccttttggag gtgcaggtat
     6901 tgatgctgca gggcacccct tacctgatga aaccctcaag gcatgtagag aagcagatgc
     6961 cattctccta gcggctatcg gtagtcccca gtatgatggg gcagtggttc ggcctgaaca
     7021 aggactcctg gctctccgta aggaactcaa tctctatgcc aatattcgcc cggttaagat
     7081 ttttgagagt ctcaagcatt tgtcaccact aaaatcagaa cgaattgctg gggttgactt
     7141 tgtcattgtg cgggagttga caggcgggat ttactttggg gatcatatcc ttgaagaaag
     7201 aaaagcgcgt gatatcaacg actatagcta tgaggaagtg gagcggatta ttcgcaaagc
     7261 ctttgaaatt gcaagaaatc gcagaaaaat cgttactagt atcgataagc aaaatgttct
     7321 agcgacctca aaactctggc ggaaagtagc tgaggaagtc gcacaggatt tcccagatgt
     7381 aaccttggaa caccagctgg tagactcagc tgctatgctt atgattacca atcctgctaa
     7441 gtttgatgtt attgtaacgg agaatctttt tggagatatt ttatctgatg aatcaagcgt
     7501 cttatctggt acacttgggg ttatgccatc agccagtcat tctgaaaatg gaccaagtct
     7561 ctatgaacct attcacggtt cagcacctga tattgcaggt caaggaattg ccaatcctac
     7621 ttccatgatt ttatcagttg ccatgatgtt gagagatagt ttcggacgtt atgaggatgc
     7681 agagcgtatc aaacgtgctg ttgagacaag tctggcggca ggaattttaa cgagagatat
     7741 aggaggtcag gcttcaacaa aggaaatgac ggaagctatt attgcaaggt tatgaagtta
     7801 gacgaaaaaa ttgctctagt ccttttgatt tggaatgtca tcattttctt gatttatggc
     7861 attgacaaat tcaaggcaag gagaagagct tggcgcatcc aagagaaaat cttactcatt
     7921 ttagccctta cttgtggtgg ttttggggcc tggttagctg gaatcacttt tcaccacaag
     7981 actcgaaaat ggtactttaa aacagtttgg tttcttggga tggtgaccac actagtagcc
     8041 ttatatttta tttggaggta atggatggca gggtcttcga gggaacacgc tgcttgggtt
     8101 ctagcggact atggttttaa ggtcgtgatt gcaggatctt tcggtgacat tcattacaat
     8161 aatgaactca ataatggcct gttgccaatc gttcagccta gagaggttag agagaaacta
     8221 gcccagctaa aaccaaccga ccaggtaact gtggacttgg aacaacaaaa aatcatctca
     8281 ccagttgaag aattcacctt cgagatagat agcgagtgga aacataaact cctaaatact
     8341 ttggatgata tcggtattac cttgcagtat gaagagttga ttgctgctta tgaaaaacaa
     8401 cgaccagcct actggcagga ttagaaaaaa tagaaaagga aatatagtaa actgaaataa
     8461 gatgtaaaca aatgaattag agcttaacat ccatttccag caatttttta gaaactacag
     8521 tggactattc tggattcaac acattataaa attatgacaa aacacattca caagaaggct
     8581 acgacatttt aaaaggtgag ggcggatgta tcgtttgccc tactaaagtt ggttacatta
     8641 tcatgaccag tgacaaggca ggacttgagc gtaagttcgc agccaaagaa cgtaagcgta
     8701 acaaaccagg tgttgttctc tgcggtagca tggatgaact tcgcgcttta gcgcaactca
     8761 acccagaaat tgaagcattc tactaaaaac attgggatga agatattctt cttggttgta
     8821 tccttccttg gaaaccagaa gcctctgaaa aactcaaagc atacggggat ggccgtgaag
     8881 aacttatgac tgatgtacgt ggtactagct gttttgttat caagtttgga aaagcaggtg
     8941 aacaattggc tgccaagctt tgggaagaag gtaaaatggt ctacgcctca tctgcttcaa
     9001 tgacaaaacg attgaaactc gctatgagag caaggtgtaa tggtgtctat ggtcgataag
     9061 gacggcaaac tcatcccaga acaaggagga gcacgttcaa cttcaccagc accagttgtg
     9121 atccgtaaag ggcttgacat tgataaaatc atgatgcacc tgtcagacac ctttaactca
     9181 tgggattacc gtcagagaga gtattattag gataaaaaag aagtctagtg ttatgagata
     9241 ttaaagctcc taacacgggg ctttttgttt agaatttctt ttctttttct ataggatatg
     9301 gtattctatg tagaaaatat atgttaataa gtaatgccaa tatttaaaca tcattagtaa
     9361 aaggagttag attgatgaat aaaagaaaag ttagtttaga agatttttat aaatggtata
     9421 gtctaaataa agaagagtta ttaaataagg caactgttgg tgaaaagttt aatgataaat
     9481 taaaagaaga gtttctccag gaatggcctt tggataggat tttaacaatg tcaatcgatg
     9541 aatatgtaat aggaaaggga cagcaaaata agtctttatg ctacgctctt gagaagggaa
     9601 aatacaaaaa tctatttctt ggaatttctg gtagctcagc ttcaaaattt ggtatttatt
     9661 ggaataaaaa aacaaacaaa tataaagatc aagctaataa tgagatttca gagttggatc
     9721 agcgattttc aaaattaaaa tcagatttgt atgaaattat caaagaaggt attcgtttta
     9781 actttgaaaa tcctattttt gatatgaaaa gatcaacaaa tgaatttatt ggtcgttctg
     9841 ctatggtgac aaaattactt tgtatctatt ctgagggaga tcctttcttt ggtgtaaata
     9901 ttaatagtca gaaagaattt tggaaccact ttgtttctca gacaaatcaa ggtggacctt
     9961 atctgcaaaa tcataaaata attgaactgg tgtccaaaac ttatcctgag ttggagccat
    10021 cgaaattagg aactatgctt tttgagtatt ctaagctttt tatggaaaat aaggaagaca
    10081 atagtacaat ggattcatca aacaattttc gtcatcaatt aactcaatct ctattaaagt
    10141 ctccaaacct tatccttcgt ggtgctcctg gcacgggaaa aacttatctt gctaaagaaa
    10201 ttgctaaaga attaacggat ggcaacgaag accaaatcgg atttgtacag tttcatccat
    10261 cttatgatta tacggatttt gttgagggtt taagaccgga ttcaaatgag gatggcagta
    10321 tattttttaa attaaaagaa ggtattttta agaagttttg tcagaatgct atagattcta
    10381 agaagattgg aggtcaagat aattttgatg aagctaggga tctttatctt gagtatgtaa
    10441 ataacataga tgaaaaagaa tatttgacag aattttccta tctaacagta aatagtcgaa
    10501 ataattttaa tatcaattat gaaactaaag ccaaaggaac tgtcttaaca aaatcttatg
    10561 tttatgaact atataaagat gaaaactatc tgaaacagcc ctattatcat aaccaaggaa
    10621 aaaaagtcct tgaaacttta aagaaaagat ttggtttgaa agactatatt tctccagaag
    10681 aggttgaaac agacaagaat tttgtcttca tcatcgatga gatcaatcgt ggggagattt
    10741 ctaagatttt tggcgaactc tttttctcta tcgaccccgg ctatcgtggt gaaaaaggaa
    10801 gtgtttctac ccaatatgca aatctacacg aaactgatga aaagttctat atccccgaaa
    10861 atgtttacat catcggaact atgaatgata ttgatcgttc agtggatacc tttgattttg
    10921 ctatgcgtcg tcgttttcgt tttgttgaag ttactgtcga gggtcaagct ggcatgttgg
    10981 ataaagagtt gaatatccat gcagaagaag caaaaattcg tctaagaaac ttgaacgctg
    11041 ctatcgaaaa tattcaggaa ttaaacagtc attatcatat tggaccaagt tattttctta
    11101 agttgaagga tgtagatttt gactatgaat tactctggtc tgattatatt aagcctctcc
    11161 tagaagacta cttgcgaggt tcttatgatg aggttgaaac tttggaaact ttgaaaaaag
    11221 catttgatct gacaaataat gagcaaaaag atcaggcagt agctgatgac aatgaaggcg
    11281 atgaaaacga tgatgcggat tactgataat caacacaaga ttattaaaga aaaatttgtt
    11341 gaagaatatc ctaaactaag caatcctctt ttagacagaa ccttggaaag tctatcccaa
    11401 gatgaacgta ttttcatttt tccaaatgat ttgactcata ctcctgattt ggataaggac
    11461 caaaagattt ttgaaacagt caatcagaaa atcaagacag ggaacgtgat tggttttctt
    11521 ggatatggtc aggaaagatt aacgatttcc tcacgatttt ctgatgagag taatgaccat
    11581 tttttgcatt atctcttaaa caaggttctt catatcaatc tcactagttt agatgttgct
    11641 ttgtctcgtg aagagaggct ttatcaactt ttgatgtatc tcttccccaa gtatctacaa
    11701 gctgctattc gaaaaggtct ttataaggaa tatcatcgat tttctcataa cgacagtcat
    11761 gttaagggag tgattgatgt aagaagccat ctcaagaaaa atcttccttt cacgggaaat
    11821 attgcctaca caacgagaga gttcacctat gataatcccc tcatgcagtt ggtccgtcac
    11881 actattgaat acattaagaa tcagaaaagc attggtcaag gggtactaga taatctctca
    11941 actagtcgtg aaaacgtatc tgaaatcgtg cgtgtaacgc cctcttataa actagctgat
    12001 cgtgctaaga ttattcgggg aaatcaatct aaacctatac gtcatgcata ctttcacgag
    12061 tacagaaact tacaagaact ttgtctgatg atcctaaacc aagaaaagca cggtttaggg
    12121 tatcaagatc aaaaaatcta tggtattctc tttgatgttg cctggctttg ggaagagtat
    12181 gtttacacct tgttgccaaa aggttttgta catcccagaa ataaggataa gacggatgga
    12241 atttcagtat tttctgttgg gaaacgaaaa gtatatccag atttttatga cagagaacga
    12301 aagattgttc tagatgcaaa atataaaaaa ctggaattga ctgaaaaagg aatcaaccgt
    12361 gaggacttat tccagctgat ttcctattct tatattttaa aagctgagaa ggctggactg
    12421 atttttccta gtatggagca gtcagtaaat agtgaaatag gaaaagtagc tggctatggt
    12481 tctcaattga agaagtggtc tattcgaatc cctcagaatg cctcatttta tagtgcattt
    12541 tgtaaaatga tggaaaattc agaagaaaat tttaaagcga ttattgatga agaagtgggg
    12601 agaaaataat ttcccctctt tctttcattt ttaacacaag taaaacccga acaatatatt
    12661 tcaattcata aaataatttg acaaaaactg tactttggtt tataataaat taaggaaacg
    12721 tcggaaaagg cgtagtgatg caagagcata ggtaggtcat tacaaaagaa acgagacatc
    12781 gatatgttaa atgaatttcc aatttttgat tacgaagata ttcaattgat tccaaataaa
    12841 tgtgtcatta aaagccgtgc agaagcggat acaagtgtca ctttaggaaa tcacaccttt
    12901 aaactacctg ttgtgccagc gaatatgcag acgattttgg atgaaaatgt agcagagcaa
    12961 ctggctaaag gtggttactt ctacattatg caccgttttg atgaggtagg acgcattcct
    13021 tttattaaac gaatgcacga tcaagggctc attgcttcta tctctgtcgg tgttaaggat
    13081 tatgagtatg attttgttag ccagctcaag gctgatgctc ctgagtatat cactattgat
    13141 attgctcatg gtcatgcgga tagcgtgatt tctatgattc aacacatcaa gaaagaattg
    13201 ccagatacct ttgtcattgc tggaaatgtg ggaacaccag aagctgtgcg tgaattggaa
    13261 aatgctggtg cagatgctac taaggtcgga atcggtcctg gtaaggtttg tatcaccaag
    13321 gttaagactg gttttggtac aggtggttgg cagttggctg ctctacgctg gtgtgccaag
    13381 gttgcgcgta aaccgattat cgctgatgga ggaattcgta ctcacggtga tattgccaag
    13441 tctatccgct tcggtgctag catgatcatg attggttccc tctttgcagg acatattgaa
    13501 agtccaggga aaacgattga agtcgatggt gaacagttca aagaatatta tggttcagcc
    13561 tcacaatatc aaaaaggtgc ttacaaaaat gtggaaggca aacgtatctt gcttcctgct
    13621 aaaggtcatc tgcaagacac tttaactgag atggaacaag accttcaaag tgctatttct
    13681 tatgcaggtg gacgtcaggt tgctgacctt aaacacgttg attatgtgat cgtgaaaaac
    13741 tccatctgga atggggatgc ttcccactaa gacggggtct ttgaccaaaa aacttgttat
    13801 tagagcaaat ttctgttata ataaaacaag tttccaccct tagtgtaatg gatatcacgt
    13861 aagattccgg ttcttgagat gggggttcga ttccctcagg gtggatgtaa acattctaaa
    13921 aagcctttaa tagggctttt ttctttacac tgctctaaat ttaccgctaa aactgagaat
    13981 ctctgtctaa agaatttcca aatgctttcc agtcccgttt tagagtgact cagggggctt
    14041 tttttgatat aataaaaagg actgttatca gttagaaaga ggttggtatg aaagaattac
    14101 aaactgtact aaagaaccat tttgcaatcg aatttacaga caaaaagtta ctggaaactg
    14161 cctttactca tacgagttat gccaatgagc accgcctctt aaaaatttca cataatgaac
    14221 gcttggaatt tttaggagac gctgttctac agttattgat ttcagaatat ctgtataaaa
    14281 aatatcctaa aaagcctgaa ggtgacctat caaaactccg tgctatgatt gtccgtgagg
    14341 agagtttagc tggttttgcg cgtgattgcc agtttgacca gtttatcaag ttgggtaaag
    14401 gggaagaaaa atctggtggt cgcaatcgtg acaccattct tggtgatgcc tttgaagcct
    14461 ttcttggtgc ccttcttttg gataaggatg tggccaaggt caaggaattt atctatcaag
    14521 tcatgattcc taaggttgaa gcaggcgaat ttgagatgat tacagactat aaaactcatc
    14581 tccaagagtt acttcaggtc aatggtgatg tggctattcg ttatcaggtg atttctgaaa
    14641 cagggcctgc tcacgataag gtctttgatg tagaagttct tgttgaaggt aagagtatcg
    14701 gtcaaggcca aggtcgttct aagaaattag cagagcagga agctgccaaa aatgccgttg
    14761 agaaggggct ggattcatgt atttaaagga aatcgaaatt caggggttca agtcttttgc
    14821 tgataagacc aaggtcgttt ttgaccaagg tgtgacggca gttgttggac ccaatggatc
    14881 tggaaagtcc aatattacag aaagtctgcg ttgggctttg ggggagtcta gtgtcaagag
    14941 tctccgtggg ggcaagatgc cggatgtcat ctttgctgga acagaaagtc gcaaaccgct
    15001 caattatgct tctgtagttg tgactctgga taatcatgac ggatttatca aggatgcagg
    15061 tcaagaaatc agggtggaac gccatatcta tcgtagtgga gatagcgaat acaagattga
    15121 cggcaagaaa gtccgtctgc gtgatattca tgacctcttc ttggatactg gattgggacg
    15181 agattccttc tctattattt cccaagggaa ggttgaggag atttttaatt ccaagcctga
    15241 ggaacgacga gctatttttg aagaagctgc tggagtttta aaatacaaga ctcgcagaaa
    15301 agaaaccgag agtaaactgc agcaaactca ggataatctg gaccgcttag aggacattat
    15361 ctacgagttg gataatcaaa tcaagcctct tgagaagcaa gctgagaatg cccgtaagtt
    15421 tttagacttg gaaggacaac gtaaggctat ttatttagac gttctggttg ctcaaatcaa
    15481 ggaaaataag gctgaactag agtcgacaga agaagagttg gctcaggttc aagaactctt
    15541 gatgagttat taccaaaagc gtgaaaaatt agaagaagaa aatcaaactc ttaaaaagca
    15601 acgccaagat ttacaggctg aaatggccaa agaccaaggc agtttgatgg atttgaccag
    15661 tctgattagt gacttagaga gaaaattagc cctatcgaaa ctggaatctg agcaagtagc
    15721 cctcaatcaa caggaagcac aagcccgttt ggctactttg gaggataaga gaagttcact
    15781 cagccaagaa aaatctgata aagaaagctc tttagccctc ttggaagaaa atctagtcca
    15841 aaataatcaa aaactcaatc gattagaggc tgaattgcta gctttttcag atgatcctga
    15901 tcagatgatt gagctcttac gtgaacgctt tgtggccctt ttacaagaag aagcagatgt
    15961 ctcaaaccaa ctgacccgta ttgagaatga gttggaaaat agtcgtcagc tttctcaaaa
    16021 acaagcagat caactagaaa agctgaaaga acagttggct acagctaaag agaaggctgg
    16081 tcagcaaaag gctgagcttg aaactgccaa ggagcaggtt cagaaattat tggccgacta
    16141 tcaagtctgt gctaaggagc aagaggagca gaaaacttcc tatcaagctc aacaaagtca
    16201 actctttgac cgtctggata gtctcaaaaa caagcaggct agagcgcaaa gtttggaaat
    16261 tatcctgaga aatcatagta acttttatgc aggtgttaag agtgttctcc aagagaaaga
    16321 tcgtctaggt gggattattg gagcagttag tgagcacctg acctttgatg tgcattatca
    16381 aactgcccta gagattgcgc ttggagcgag tagccaacat atcatcgtgg aagatgaaga
    16441 gtcggcaacc aaggcgattg atttactcaa acgaaacaga tctggtcgtg caaccttcct
    16501 tcctttgacc actatcaagg ctcgtacgat ttctagtcag aaccaagata ctattgctgc
    16561 aagtccagga tttctgggca tggcagatga gctggttact ttcgatacta gactggaagc
    16621 cattttcaag aatttgctag ctacaacggc catttttgat accgtggagc atgcgcgtgc
    16681 agcagcgcgc caggttcgtt atcaggttcg tatggtgaca ttggacggaa cagagctgcg
    16741 cacaggtggt tcttatgcag gtggagctaa tcgccagaat aacagtattt tcatcaagcc
    16801 agaactggag caattacaaa aagaaattgc tgaagaagaa gcaagtttgc gttcagatga
    16861 agtgagtttg aagaacttgc aagatgagct agctagattg acagaaagat tagaagccat
    16921 caaatctcag ggagagcagg ctcgtattca ggagcaaggc ttgtccctcg cctatcagca
    16981 gaccagtcag caagttgaag agctagaaac tctttggaaa ctccaagaag aggaattaaa
    17041 tcgtctttct gagggagaat ggcaagcgga taaggaaaaa tgccaagagc gccttactac
    17101 aatcactagt gaaaagaata atctggaagc tgagattgaa gagattaagt ctaataaaaa
    17161 cgccatccaa gaacgttatc aaaacttgca ggaagagcta gctcaagctc gtctacttaa
    17221 gacagaactg caagggcaaa aacgttatga agttgctgat attgaacgct taggtaagga
    17281 actagataat cttgatattg agcaagaaga aattcagcgc cttcttcaag aaaaggttga
    17341 caatcttgag aaagttgata cggatttgct cagtcaacag gctgatgaag ccaaaactca
    17401 aaaaacaaat ctccaacaag gtttgattcg caagcagttt gagttggatg atattgaggg
    17461 tcaactggat gatattgcta gtcatttgga tcaggctcgc cagcagaatg aagagtggat
    17521 tcgcaagcaa actcgtgctg aagccaagaa agaaaaggtt agcgagcgct tgcgccatct
    17581 acaaagtcaa ttaacagacc agtaccagat cagctacaca gaagctttag aaaaggcgca
    17641 tgagttggaa aatctcaatc tggcagagca agaagttaag gatttagaga aggctatacg
    17701 ctcactgggt cctgtcaatt tagacgctat tgaacagtac gaagaagttc acaaccgtct
    17761 ggatttccta aatagccaac gtgatgatat tttgtctgcg aaaaacctgc tccttgagac
    17821 cattacagag atgaatgatg aggtcaagga acgctttaaa tcaacctttg aggtcattcg
    17881 tgagtccttt aaagtgacct tcaagcagat gtttggtgga ggtcaggctg acctcatttt
    17941 gactgaagga gacttgctga cagctggggt tgaaatttct gtccaaccac caggcaagaa
    18001 aatccagtct cttaatctca tgagtggtgg tgaaaaagcc ctatcggctc ttgccttgct
    18061 cttctccatt attcgtgtca agactattcc atttgttatc ttggatgagg tggaagctgc
    18121 gctggatgaa gccaatgtta aacgttttgg ggattacctc aaccgctttg acaaggacag
    18181 ccagttcatc gtcgtaaccc accgtaaggg aaccatggca gcggctgatt ccatctatgg
    18241 agtgaccatg caagaatcgg gtgtttcaaa gattgtttca gttaagttaa aagatttaga
    18301 aagtattgaa ggatgacaat taaactagta gcaacggata tggacggaac cttcctagat
    18361 gggaatggac gctttgatat ggatcgtctc aagtctctct tggtttccta caaggaaaaa
    18421 gggatttact ttgcggtagc ttcgggtcgg ggatttctgt ctctagaaaa attatttgct
    18481 ggtgttcgtg atgacattat tttcatcgcg gaaaatggca gtttggtaga gtatcaaggt
    18541 caggacttgt atgaagcgac tatgtctcgt gacttttatc tggcaacttt tgaaaagctg
    18601 aaaacttcac cttatgtaga tatcaataaa ctgctcttga cgggtaagaa gggttcatat
    18661 gttctagata cggttgatga gacctatttg aaagtgagtc agcactataa tgaaaatatc
    18721 caaaaagtag cgagtttgga agatatcaca gatgacattt tcaaatttac aaccaacttc
    18781 acagaagaaa cgctggaaga tggggaggct tgggtaaacg aaaacgttcc tggtgttaag
    18841 gccatgacaa ctggctttga atccattgat attgttctgg actatgtcga taagggagtg
    18901 gccattgttg aattagttaa aaaacttggt atcacaatgg atcaggtcat ggcttttgga
    18961 gacaatctta atgacttaca tatgatgcag gttgtgggac atcctgtagc tcctgaaaat
    19021 gcacgacctg aaattttaga attagcaaag actgtgattg gtcaccataa ggaacggtcg
    19081 gttatagctt atatggaggg cttataatgg cagatataaa attgattgca ttggacttgg
    19141 acgggacctt gctgactact gataaaaggc tgacggatcg taccaaggaa accttgcaag
    19201 ctgcgcgtga tcgtggtatc aaggtcgtat tgacaactgg tcgtccctta aaagccatgg
    19261 atttctttct ccatgagtta gggactgacg gtcaggaaga tgagtatacc attactttta
    19321 atggtggatt agttcagaaa aatacaggag aaatccttga taaaacagtc ttttcatatg
    19381 atgatgtggc acgtttgtat gaagaaacag agaaattatc actgcctctt gatgccatct
    19441 cagaaggaac agtttatcaa atccaatcgg accaagaaag tctttatgcc aaattcaatc
    19501 cagctttgac ctttgttcca gtggactttg aagacttatc tagtcaaatg acctacaaca
    19561 aatgcgtgac tgcctttgct caagaaccct tggatgcagc cattcagaag atttctccag
    19621 aattgtttga ccaatatgaa atctttaaat cacgtgaaat gttgctagaa tggtcaccaa
    19681 agaatgttca taaagcaaca ggtttggcaa aactaatcag ccatcttgga atcgaccaaa
    19741 gtcaagtgat ggcttgtggt gacgaggcca atgacctctc tatgattgaa tgggcaggtc
    19801 ttggtgttgc tatgcaaaac gctgttcctg aagtaaaggc agccgcaaat gtagtgacgc
    19861 cgatgaccaa cgatgaggaa gctgtcgcct gggctatcga agaatatgtg ctaaaggaga
    19921 actaagatat gggattgttt gaccgtctat tcggaaaaaa agaagaacct aaaatcgaag
    19981 aagttgtaaa agaagctctg gaaaatcttg atttgtctga agatgttgat cctaccttca
    20041 cagaagttga ggaagtttct caggaagaag cagaggttga aattgttgaa caagctgtgt
    20101 tccaagaaga ggaaatccaa gacacagttg aagaaagtca ggattcagag ccagctgtag
    20161 gggtttctca agaagaaata gaagaattac caaactcaga agaattacca gaggaagaga
    20221 agcttgagca cgaaggaact gtagaagaaa ataattctga agtgcttgaa ccagaagctc
    20281 ctcaaacaga agaaactgtt caggaaaaat atgaccgcag tcttaagaaa actcgcacag
    20341 gtttcggtgc ccgcttgaat gccttctttg ctaacttccg ctctgttgac gaagaatttt
    20401 tcgaggaact ggaagaactg ctgattatga gtgatgttgg tgtccaagtc gcttctaact
    20461 taacggagga actacgttac gaagccaagc ttgaaaatgc caagaaacct gatgtacttc
    20521 gtcgtgtcat cattgagaaa ttggttgagc tttatgaaaa ggatggtagc tacgatgaaa
    20581 gcatccactt ccaagataac ttgacagtta tgctctttgt tggtgtgaat ggtgttggga
    20641 aaacaacttc tatcggaaaa ctagcccacc gctacaaaca agctggtaag aaggtcatgc
    20701 tggttgcagc agataccttc cgtgcgggtg cagtagctca gctagctgaa tggggccgac
    20761 gagtagatgt tccagtagta actggacctg aaaaagctga tccagccagc gtggtctttg
    20821 atggtatgga acgtgccgtg actgaaggta tcgatattct catgattgat actgctggtc
    20881 gtctgcaaaa taaggataac cttatggctg agttggaaaa gattggtcgt attatcaaac
    20941 gtgttgtgcc agaagcacca catgaaacct tcttggcact tgatgcatca acaggtcaaa
    21001 atgccctagt acaggccaaa gaattttcga aaatcacacc tttaacggga attgttttga
    21061 ctaagattga tggaactgct cgaggaggtg tggttctagc cattcgtgaa gaactcaata
    21121 ttcctgtaaa attgattggt tttggtgaaa aaatcgatga tattggagag tttaactcag
    21181 aaaactttat gaaaggtctc ttggaaggtt taatctaatc agaagcaaaa atcctgcaag
    21241 gcataaactt gcaggaaatt tttttattct aagcgaccat cttgacgata ggtgatatct
    21301 ggttgccaag tccatttggc accgaatttt tcaagtaggt caaagctggc ttgaggtccc
    21361 atgcttccag ctttatagtc atgaagtggg gcaccatttt cagcccagag cttttcaata
    21421 cggtcaatca acttccatga cgcacaaact tcatcccagt ggctaaagtt agttgagttg
    21481 ttatttagga catcataaat caatttttcg tatggttctg gagaagcacc agttgcagtc
    21541 gcatctgtac ggtaatcaag tgagttagga gccaagttaa attcttctcc tacttgcttc
    21601 ccatttaggc taagagagaa gccttctgtt ggttgaatat agatggtcaa aatatttgga
    21661 gcaagtggtt ctccaaagat agaatccatt tgtttaaaga cgatgttgac atgagttcct
    21721 ttttcagtca gtcgtttacc tgtacggaaa aagaaaggaa caccacggaa tcgatcgctg
    21781 tctacaaaga aggcaccaga tgtaaaggtt tcagttgttg attctggatt cacatttggc
    21841 tcgctacgat aagagatgta tttcatgcca tcaatcttac cagagcggta ttgcccacgg
    21901 ataaagtgtt ctttgagttc ttcatcagtt ggatgataga ggtttttaaa gaccttaatc
    21961 ttttcagcac gaatctcgtc ttttgtgaag cttgctggtt tgtccatggc gaggagcgaa
    22021 agaagttgta gagtgtggtt ttggaccatg tcacggaggg caccggattg gtcatagtag
    22081 ccaccacgtt cttctacacc caagcgctcc gcaaaggtaa tttgaacatt gtcgataaaa
    22141 tccttgttcc aaacgttttc aaaaatcaag tttgcaaagc gaactgcaaa gatgctttgg
    22201 atcatttcct taccaagata atggtcgata cggaaaattt gttcttcgtc aaatgttgct
    22261 aggagttcgt cattcaactt gcttgcagtt gcgtaatctg taccaaatgg tttttcaacg
    22321 atcaagcgct caaaaccttt gccatcgaca atgttttcag atttgaggtg tttggcaatg
    22381 gttccaaaga actgaggtgc catagacaag aagaagagct tattgtgttc agcttggtat
    22441 ttttcattaa gctcagcttg taattgacgc aaagcaatat aatgttccga atcattgaca
    22501 tcatggcttt gatagtagaa gtggctagca aattcttggg cttgctcggt actatctgcc
    22561 aaatcaagga tggactcgac aactacagat tcaaaatatt ccttactcca aggtctacgg
    22621 gcagttccaa taacggcaaa gtgcttggaa agattgccgg attgatatag tctaaaaagg
    22681 gaagggtaga gcttgcgttt agccaggtct ccactcgcac cgaaaattgt aacaataacc
    22741 ttagatgaca tctagctacc taatttctat ttttattcct agtcttgcta ggtatgagga
    22801 aacctcattt cataaactta attctaacat aattttccga aaaatcaaaa attccaatac
    22861 gaggtagaaa aactgcaagg aaatccttac agtttgagtt tagacgttta agaccttatc
    22921 taagaactct ttcagacgag ggtgttgtgg gttatcaaag atttggtcag gtgttccgtc
    22981 ttcaaggaac tcgccatctg cagtaaagat aacgcggttg gcaacctgac gagcaaatcc
    23041 catctcatgg gttacgataa tcatggtcat gccttgctca gccaattcct tcataacgtt
    23101 aagtacgtct ccaaccatct cagggtcaag ggcagaagtt ggttcatcga agagcatgat
    23161 gtctggattc attgctaggc cacgagcgat ggccacacgt tgtttttgac cacctgatag
    23221 gctatctgga ttggcattag ctttatctgc tagtccaacc ttttcaagca actccattcc
    23281 caattcctca gcttcttcct tagtcatcaa cttgtgctca ataggagcaa aggtgatgtt
    23341 gtccaataca gacatatgag ggaagaggtt gaagtgttgg aataccatgc cgatattttc
    23401 acggacgtgg tcaacattgg ttgttttttc agttaaatca tagccgttca cagtgatgtg
    23461 accgctagtg acttcttcta aaagattgag gctacggagg aaagttgact taccagaacc
    23521 tgaaggaccg atgatacaaa caacatctcc ttcatagaac ttagtcgtaa ttccttttag
    23581 gacttcattt tttccatagt gcttgtgtaa atcatttaca tcaattttta attttgccat
    23641 taacgaatcc tcttttctaa gcgtttcgct agtctagtca aaagtgtgat aattacaaga
    23701 tagaagatag caaggattgt atacatcttg aaactttggt agttacgagc aatgataatc
    23761 ttaccagttt ggaagagttc aaccaaaccg atagcagata cgatagttgt atctttaaga
    23821 gcgataacga attggttgac aaagtttggc aacatcaatt tagttgcttg tggcaagata
    23881 atcttacgca tggtttttcc ataagagata cccaagcttc ggctggcttc catttggcca
    23941 actggaacgg cctgaatacc accacgaacg atttcagcga tataagcagc cgcattgagt
    24001 gagagggcaa tggttccagc tacaaagtcg ttaattgggc tttgttggcc tgtgatagac
    24061 tcgatgaagt ttggaattcc ccagaagatg aaggctgcaa gaatcatcaa tggaatacca
    24121 cgaataacgt caacgaaaat ctcagagatg acgcgaagag atttgtatgg gctaacgcta
    24181 aacataccga agataattcc gatgacaatg gcaatagcaa atgagataag agctagagca
    24241 agagtgatac caagaccgct aaggagttgt ttgtagttgt tttgaagcaa gccccagagc
    24301 gttgtttcgt caacagtact tgttgaagca gttgaagatt cgctagctag gtatttgtca
    24361 agaatctttt ggaattcacc gtttgcttta aggtttgcaa gtccgttgtt gaacatttca
    24421 atcagttctg gatttgctcc ttttttaacg gcaaaggctg tttcaccgat tggagttcca
    24481 gagattggag ttttcaattt ttgaccttgg ctgatagaat atttgagaac aggttcatca
    24541 tccataacgg catcaatggc accagtgttt aaactgtcat acattgaaga accatcagca
    24601 aaggttttga ttttgtagcc gtatttgctt tgattttctg ttaggaaggt ttgagaagca
    24661 gttccgtttt taacaccgac tgtctttcct tttagatctt cataagaagc aatattgctt
    24721 gattctttga caccaagaat ggtattagca gtgtagtatg attctgagaa gtcaaaagtt
    24781 gccttacgag catctgtgac agacatacca gcgatgatac catcggcttg accagcttgg
    24841 acagcactga tagcagcatc aaaaccaggg ttggtgattt caatttcaaa accttggtct
    24901 ttagcgattg ccttaatcaa ttccatatca ataccagtgt attggttgct tgaattttgg
    24961 aaaacaaaag gtgcaaaaga agaatcgctg gcaatgatat atttagcctt aacaggaata
    25021 gcttttaatc ctgcgagagt agttgtagtt ggcactgctg aagatgatga agcagtccat
    25081 ttcttgataa ttttatcaag actaccatct tttttcattt cagacaaggc ttggttaaat
    25141 tcagtaacca ggtgctcgta tttacttcct tttttcacac cgaaagcaaa acttcctaca
    25201 gcttcaccat ccatttcaat atggaggtct tgaccttggt taatggcata ttcgataaca
    25261 ggtttgtcat ccatcatggc atcgatggca ccagcactca agctgttgtt cattaaatca
    25321 ccagtgtcaa atgttttaat agtaaagccg tatttatctt tgattgtttc aaggaaacgt
    25381 tgagcggcag ttccgttttt aacaccaacg gttttgccag ttaattggtc gtacttgcta
    25441 attttgtgtg actttgtagt agcaatgaca acttttgtat catagtaagt atcagacatg
    25501 gtgaagactt tttcacgttc tttagtcttt gtcatccctg ccatgatagc gtcggcttgc
    25561 ccagcttgaa ccgcattgac tgctgcgtca aatccaggat aggacatctg aatgttccag
    25621 cctttaatct cagcgacttt gttaataatg tcaacatcaa ttcctttata agtttgatct
    25681 gaatctttaa actcaaaagg tgcataggcg gtatcagaaa caatcttaat cgtttctgct
    25741 ttggcaatac ctaatgagaa aattgggaat aaaattagca aaaatgctag aaattttttc
    25801 ttcattttta gtctcctttt ccgaatattc tctcattata tcagaaaaag taaaaaaagg
    25861 caaattttta tatttttcgt gtcagaaaat ataacatcga ttttttcctt tccttgaatt
    25921 gctattaaaa agtgctataa taatagtata tatagaagaa aagaggacgg ggatatgaag
    25981 aacaaaagaa tatttaaaga cttccaagct tcaaaaatga gtttaaacat ttacacaagc
    26041 cccttgttag cctttgtttt tgtcttcata ggagagtttg tggcttttac tttgtatggt
    26101 attggcttgt tagctctcat cggacttgct agaaattttg gagaggctgg tcaaaatctt
    26161 gcaagctact tgcagacctt gcatcagagc ttgacggata aaacaagtga ctttcgttta
    26221 attttaggat tactggcctt tggttttatt cttaacactg tgttcagatg gacaagaaaa
    26281 gttgagaaaa gacctattcg aaccttggga ttttatagag agaatttcct cagcaatctt
    26341 ctgaaaggat ttagtctagg cctggcactt tttcttctga ccttgttagg tttagtggtc
    26401 ttaggtcaat atcgtttgga atccattcac ttgaatcctt attctcttgc ctttgtcgtc
    26461 tttactatcc cattttggat tttacagggg acagcagaag aagtggtggc ccgtgcttgg
    26521 ctacttcctc aattggcctc aagaaccaat ctaaaactag ctattcttat atctagcctg
    26581 ttctttaccc tgcttcatat gggcaattct ggcctcaccc ctctatctct agtaaatctc
    26641 tttttattcg gagttgccat ggctctttac cttctcaaaa ctgatacagt ttggggtgtt
    26701 gcaggtattc atggtgcttg gaattttgct cagggtaatc tctttgggat tttagttagt
    26761 ggtcaaccgt caggaacgtt tctgatgacc tttttaccac aaggcaatca agattggcta
    26821 tcaggtggtt cttttggcat agaaggttcc attatgacaa gtctggtatt actactgctg
    26881 attgtctatc ttgctaataa attaaagaaa gaaaatgaaa ggatgtgact tcggtccgtc
    26941 cttttcttcg tgaaaatact ataagtatgc taaaatagga atagcacatg gagagaggat
    27001 tcttatgatc aatcacatta cagataatca atttaaacta gtatcaaaat atcaaccatc
    27061 aggagatcaa ccccaagcta tcgagcagtt ggtggataac attgaggggg gagaaaaagc
    27121 tcagattctg atgggggcga ctggaacagg gaagacctat actatgagtc aggtcatttc
    27181 taaagtcaat aaaccaactc tggttattgc ccacaataaa actctggctg gtcagctcta
    27241 tggggagttt aaggaatttt tccctgaaaa tgcagttgag tatttcgtat cctactatga
    27301 ttattaccag ccagaggcct atgtcccttc tagcgatacc tatattgaga aggatagttc
    27361 tgtcaatgac gagattgaca agcttcgcca ctcagctacc tcagcccttt tggagcgtaa
    27421 tgatgttatt gtcgtggcct cagtctcttg tatctatggt ttgggttcgc ccaaggaata
    27481 cgctgatagt gtcgttagtc tccgtcctgg tctagagatt tctcgtgata aactcttgaa
    27541 tgacttggtc gatattcagt ttgaacgtaa tgatattgat ttccaacgcg gaagatttcg
    27601 cgttcgtggg gatgtggtag agattttccc agcttcccga gatgaacatg cctttcgagt
    27661 agaatttttt ggagacgaaa ttgaccgtat ccgtgaggtt gaggctctga caggtcaggt
    27721 attgggagag gtggatcatt tggcgatttt cccagctact cactttgtga ccaatgacga
    27781 ccatatggaa gttgccgttg ccaagattca agcagaatta gaagagcagt tagctgtctt
    27841 tgagaaggaa ggtaaattgc ttgaagccca gcgcttgaaa caacggacag agtacgatat
    27901 cgaaatgctg cgcgagatgg gctatacaaa tggggttgaa aattattctc gccacatgga
    27961 tggacgtagt gaaggtgagc ctccttatac gcttctcgac tttttcccag atgatttttt
    28021 gattatgatt gacgagagtc atatgactat gggacaaata aaaggcatgt acaatggaga
    28081 ccgttcgcgt aaggaaatgc tagtaaatta tggtttccgt ttaccgtctg ccttggacaa
    28141 tcgtcctctc cgtcgtgagg agtttgaaag ccatgtgcat cagattgttt acgtatcagc
    28201 gacacctggt gactatgaaa atgaacagac tgagacggtg attgagcaaa tcattcgacc
    28261 gacagggctt ttggatcctg aggtggaagt ccgtccgact atgggacaga ttgatgatct
    28321 cttaggtgaa atcaatgccc gtgttgaaaa gaatgaacga acctttatca ccaccttgac
    28381 caagaaaatg gcagaggact tgaccgacta tttcaaggaa atgggcatca aggtcaagta
    28441 catgcactcg gatatcaaga ccttggaacg gacggagatt atccgtgatc tccgcttggg
    28501 tgtctttgat gtcttggttg gaatcaatct ccttcgtgaa gggattgacg ttcctgaggt
    28561 tagcttggtt gccattctcg atgctgacaa ggaaggtttc cttcgtaacg agcgtggact
    28621 catccagacc attgggcgtg ccgcccgtaa cagtgaaggt catgtcatca tgtatgcaga
    28681 tacggttacc cagtctatgc aacgtgctat cgatgaaact gcccgccgtc ggaaaattca
    28741 gatggcctat aatgaagagc atggtatcgt accacagaca atcaagaaag aaatccgtga
    28801 cctgattgcc gtgaccaagg cagttgctaa ggaagaggac aaggaagtcg acatcaatag
    28861 cctcaacaaa caagagcgca aagaactcgt caagaaacta gaaaaacaaa tgcaagaagc
    28921 cgtcgaagtg cttgactttg aactggcggc tcagatccgt gatatgatgt tggaagtcaa
    28981 ggccttggat taggggaata gtatgattta tttaagaaag ttaaagaaag aagatttgat
    29041 gtctttatgg gaaatggctt attcacagct taatccagtt tggaaacagt atgatgctcc
    29101 ctattatgat gattatcagt atttttcaaa ttttaaagaa ttcgaactac aaaaatcaga
    29161 atccatttta agcaactcaa atcgccttgg tatttttgtt gatgataaac tagttgggac
    29221 tgtttcgcgt tattgggtat gtaaagaaac aagatggatg gaattgggaa ttggtattta
    29281 tgataaaaaa ttctggaaca ctggtattgg gaaagttgct atgttgcagt ggatagatag
    29341 gacgtttcag gattacttgg agttggagca tctgggtttg acaacttggt caggaaatct
    29401 tggtatgatg aaacttgctg aaaaattaag aatgaaaaaa gaagctcata ttccaaaagt
    29461 tcgttattat caaggtaaat attttgatag tattaaatat ggtattttga gagaagactg
    29521 ggagaaaata aatgacggtt attatcaaat caatggaaac tcctgaagag atagaaggca
    29581 aatccctcgt tcactggcaa acgtggagag agtcttatga tgaccttttg cctgcggaat
    29641 ttcaggatac aatgacatta gaaagatgtc gattctttag tcaaaagtat ccagaaaata
    29701 cattgattgc gatggatggc gtgaaggtgg ttggttttat cagttatggc aattttcgtg
    29761 atgagactat tcaagctggt gaaattattg ctttatatgt tttaaaagac tattatggaa
    29821 aaggtatcgc acaaaagtta atgaaagcag ctttgactgc tcttaatcat ttttctgaaa
    29881 ttttcttatg ggtattgaaa gataacaagc gcgccattgc tttctatcaa aaaatgggtt
    29941 ttacttttga tggacaagaa aaaatacttg aacttggaaa gcctataaag gaaaaacgaa
    30001 tggtgttcta ttctaaataa ttttaaaaaa gtaaaagata atatctctgg tagtaagtct
    30061 gaaaatttaa taaattagaa agcgagtaaa tttatgtccc gttcccaatt aacaatttta
    30121 acaaatatct gtctgattga agacctcgaa actcagcgcg tggtgatgca gtatcgcgcc
    30181 cctgaaaaca atcgctggtc tggttatgcc tttcctggag gtcatgtaga aaatgatgag
    30241 gcttttgcgg agtctgtcat tcgtgaaatc tacgaagaaa cagggttgac tatccaaaat
    30301 cctcaacttg tcggcattaa aaattggcca ctagatacag gtgggcgcta tattgtcatt
    30361 tgttataagg cgactgagtt ctctggtacc cttcaatctt cagaagaggg agaagtttct
    30421 tgggtgcaaa aagaccagat tccaaactta aatctggcct atgatatgct accattgatg
    30481 gaaatgatgg aagctcccga caagtcagag tttttctatc ctcgccgtac agaagacgat
    30541 tgggaaaaga aaatcttcta gtcttttact aaataaccta gctgatccaa ggcctcctcg
    30601 atatagtgga ggtcttgttg tgtttcggct tcgactaggt ggtaatggat gccatctgtc
    30661 aattcagata gaggtctaaa gtcagaatgc tcgacttgtt ctagaaaatg ttgcacatct
    30721 ctgcgacaag acagtttaag caaggtttca atttctccat aaacgggatg gtcaatcaag
    30781 gtattctgga catgcccacc attatcgaca atagcaagga gttcctgacc gatttcttca
    30841 acttcatgtt tcactttgaa aagtttgtga acataaagat tggtatcaat ttgcttatag
    30901 atgtagccac gattggtgga taggatagga gcgccatcgg ctctcaaaat tgcaatgtcc
    30961 tgcacaatga cctgacgtgt gacatgaaaa tgttccgcca aactttggcc attaagggct
    31021 ttaggagctt ctttcaagag ttggagcagg gcttgtttgc gatcttttgt catagctttt
    31081 ccttttaacg gcgttttcga agcactttat agacagctag tgctaatgta tagtctacca
    31141 tactatggat aattgtgcca aatccaacta gtacaaatag aacatagaac atattttcta
    31201 cattggtacc cgaagttgcg taaaaaagga cacaggctaa tacttcagca agggcatgaa
    31261 caacagccaa aacaaaattg aaaacccagg aagattttgg tttatctagg gtatcgggga
    31321 atttttgtag gtaaagagct cctaaagcac caaaagatat atgggaaaaa gcccgaaaaa
    31381 cgataaccat gggatagcca gccatcaaaa atccaaaact agaggctagg atgacaaaaa
    31441 ctgccatcaa gggcgacaag aacatggcta taaaaatagc gatgtggctc cccaaagtat
    31501 aggaagcagg tggaatgaca atcttgaaag gcataacaat tggaatcaaa atcgcaatag
    31561 ccgttaaaag ggctgtcatt gtcataaatt gtgtcttttt ccgtgtattc acaagaatct
    31621 cctttttaac tgcatataca ctagtatggt acaataaacc agacaataaa gcaagaattt
    31681 acttgggttt atagatcatt ttttagttaa aagttatagt agattgaaac tagaatagtc
    31741 cacctctact tctaaaacat tgttagaaat cgatttgact gtcctgatcg atttgtcctg
    31801 ttcttatttc gttttactat agtaaagatt tcattaaaaa gaaactgtat agagcaaaat
    31861 ctccaccttc aggtttgaaa gcggagattg tttttatttt ttcaaggttt gtagtcgtgg
    31921 aacaatggct agggttagaa ttgcagaaat gaccaattca gcaattgaat ttgttgagat
    31981 aacggttgcc agaagaagtt ggatatttcc attataaaca tttccaaata ggaagaagat
    32041 tcctccaagg acaaagatag tatttgtcaa ggaaccaaga gccccagcta aaatcagacc
    32101 agtcttgttt ttcatcagtt tatagactaa gtaaggagtt aaaccaatca aaatacgtgg
    32161 gacaatggca atgatagctg agtagatgtt tccgtttggt acgaagggag agaagaggta
    32221 gcttgtcggt agaatcgtaa tcgtgttaac cgtcaagcta agtaatccca tcaaaaatcc
    32281 aagtgtaacc ccaactcgtg gaccataaat aatgctggca ataatgacag gaatatgaac
    32341 aatggtcggt ttgattggaa atggaaaaag gttaaagata agtgagctca gaaagtgtat
    32401 cacgagcatg gtagcaaaaa agatagcaat gggtgcaata ttagagcgtt ttttcatcga
    32461 tagtttcctt tattctttct aaaataattg tgaggtcagc taaagctcct cgtccgtggt
    32521 ctccacaagc tagtagggat tccttaggag caatcagctg atagccgtag gtttctaatg
    32581 ttttcagatt attctgagtt actggatggt catacatttt tgtattcata gcaggagcta
    32641 ttagtttggg aatatgactt ggtagggcta gagctgtact ggttaccatg ttgtccgcaa
    32701 atccgtgagc tagttttgca atagtgttag cagttgcagg taccacgata aataaatctg
    32761 ctttttttcc aagttcgata tgattgacct gatcaggata gggttctttc atgacatcca
    32821 agtggacagg attctgtgag agtacctgta gtgtcaaagg ttggataaac tctgtagcag
    32881 cctgagtcat taagacagtg acttgatggc cttgtttttt tagagaactg actaaatctg
    32941 ccgacttata agaggcgatt gaacccgtta cagccaagag aatgtttgcc atagttttcc
    33001 tttctaagaa tgataggctt gaattttttc aaggaggagt tctgcaattt cttctttagt
    33061 ctggactgtt tgaagctgat ttttctcaac aaatatagct cggtgctgac ctgctgaaat
    33121 ttgagtcagg tcattcgcga tgattaaatc tgcttgattc ttgataagac tttttcgtgc
    33181 aatgtcaacc agatgatctt cggtaacatc aaccagcagt ttgaaaccaa tcagatgaat
    33241 agtaggattc cattccttga ctagggatat gattttgggt gtctttttaa ggaacaaaac
    33301 ctgaacctca tcagttgaag aaatcttggc ctgatgattc tgcttgctta aaaattcttt
    33361 tagattggag ctagcctgaa cttcctcaag ccctgtcata taaacaggag tgtagtcaga
    33421 aacagccatt gagtggatca agacctgata atcctgaaca cgttcttgca tttctattag
    33481 aaggtccttg gtattggtaa tttctcgaat acttaggtta ggatgaggct ctggcttcag
    33541 agctcgtttt gtcgtaatta aacaaacttc atgccctgca gaaagcaaag tctctgtgat
    33601 aattttcccc aagtgacctg tagaatggtt agtgatagag cggacgctat cgatagcttc
    33661 actggtaccg cccgatgtaa ctaaaatttt catagcacta ttgtacacaa aaataagcgg
    33721 taagtaaaat gaaagtgata aatttttggt aaaaacgaag atttactata gtttcaaact
    33781 ataaagccat ataaaattta agaaaggact ctaaaagtct taaaaacaga gatatttacg
    33841 aacgtttaga gagttttgag atgttaaaaa tcttgttttg tgatataatg aattatcata
    33901 taagaggtta gaggaatttt gaatgaaaac agatattgaa atcgcacaga gtattgagtt
    33961 gaagccaatt gttgatgttg tagagaaact tggtatttct tacgacgatt tggagttgta
    34021 cggaaagtac aaggctaaac tcagctttga taaaattcgg gcagttgaga gcaatccagt
    34081 cggtaaattg atcttggtta ctgccatcaa cccaacacct gcaggtgaag gaaagtcgac
    34141 gcttaccatt ggtcttgcgg atgccttgaa caagattggc aagaaaacca tgattgctat
    34201 ccgcgaaccg tctcttggtc cagtcatggg gatcaagggt ggtgctgctg gtggtgggta
    34261 tgcacaagtt ctgccaatgg aagacatcaa cctccacttt actggagata tgcatgctat
    34321 tacaactgcc aacaatgccc tttctgcctt gattgacaac cacttgcacc aagggaatga
    34381 gctgggaatt gatcaacgtc gtatcctctg gaaacgcgtt gtggacttga acgaccgtgc
    34441 gctccgccat gtgactgttg gtcttggtgg tcctctaaac ggtattccac gtgaggatgg
    34501 ttttgatatt acagttgctt cagaaatcat ggcaattctt tgcttggcaa cggacatcga
    34561 ggacttgaaa cgtcgtttgg cgaatatcgt tattggttat cgctatgacc gtacgcctgt
    34621 ttctgtaggt gatttgcagg ttgagggtgc cttggctttg attttgaagg atgctattaa
    34681 gccaaacttg gttcagacaa tttacggtac acctgccttt gtacacggtg gtccatttgc
    34741 caatatcgct catggctgta actctgtttt ggcgacgaca acagcccttc acttggctga
    34801 ttacactgtt actgaagctg gttttggtgc ggaccttggt gctgagaaat tccttgatat
    34861 caagacacca aacttgccaa catctccaga tgcagttgtt attgtcgcaa cccttcgtgc
    34921 ccttaagatg aatggtggtg tggctaaaga cgctctgact gaagaaaatg tagaggcagt
    34981 tcgtgcaggt tttgctaact tgaaacgcca cgttgaaaat atccgtaagt tcggtattcc
    35041 agcagttgta gctattaacg aatttgtatc tgatacagaa gctgaaattg cagtcttaaa
    35101 agaactctgt gcctcaatcg atgtaccagt tgaattggct agtgtctggg ctgatggagc
    35161 agaaggtgga gtagcacttg ccgaaacagt tgttaagaca attgctgaaa atccagctaa
    35221 ctataaacgt ttgtacgata atgacctttc tgtccaagaa aagattgaaa aaattgtcac
    35281 tgaaatctat cgtggtagca aagtgaactt tgagaagaaa tctcaaacac aaattgctca
    35341 aatcgttcaa aacggttggg acaaattgcc aatctgtatg gctaaaactc aatacagttt
    35401 ctcagacaat ccaaatgcgc ttggagcacc agaaaacttt gaaattacca ttcgtgaatt
    35461 ggtaccaaaa ttaggtgcag gcttcatcgt tgccttaact ggtgatgtta tgaccatgcc
    35521 aggtcttcca aaacgtccag cagctctcaa catggatgtt gaaagcgatg gaactgtact
    35581 aggcttgttc tagtggattg aaattgactt taagtgagtt tggaaataat atccaagctc
    35641 attttttctc agactgcaag cagttcatca ataagattta tactcttcga aaatctcttc
    35701 aaactacgtc agcttcacct tgccgtaggt atatgatact gactctgtca gttctatcca
    35761 caacctcaaa acagtgtttt gagcaacctg cggctagctt cctagtttgc tctttgattt
    35821 tcattgagta taagtagata gaaaagttat atactatcta ttgaaaatat tataaatcag
    35881 atacaaagac aggtgaaacc ttctgtgtta tactagagat atgttagatt tgaaagaata
    35941 cggtatcgtc atgtggccgg aggagaaagt catttctttc cgtgagaaac ttctcgcttg
    36001 gtatgatgaa aacaaaagag atttgccttg gaggagaagt aaaaatcctt atcacatctg
    36061 ggtatctgaa atcatgcttc agcagaccag ggtggataca gttatccctt actacgaaag
    36121 attcctggac tggtttccaa ctgtcgaaag tctggcaact gcgcctgagg agagtttact
    36181 gaaagcttgg gagggcttgg gctattattc tcgagttcgc aatatgcagg ctgcagccca
    36241 gcagattatg actgactttg gtggccaatt tccaaatacc tatgaaggaa tttccagctt
    36301 gaaagggatt ggaccttaca cagcaggagc catttccagt attgctttta acttgcctga
    36361 gccagctgta gatggtaatg tcatgcgggt cttggcgcgt ctgtttgaag tcaaccacga
    36421 tattgggatt ccaagtaatc gaaaaatttt tcaggcaatg atggagatct tgattaatcc
    36481 ggatcgtccg ggtgatttta atcaagcctt gatggactta gggtctgata ttgagtctcc
    36541 tgtaaatccc agacctgaag aaagcccagt caaggacttt agtgcggcat atcagaatgg
    36601 aaccatggac cgttatccaa ttaaatctcc taagaaaaag ccggttccta tttatcttaa
    36661 agccttagtt gtgaaaaatt ctcaaggaca atttttactt gaaaaaaatg aaagcgaaaa
    36721 gctcttggca ggtttttggc atttcccctt tatagaagtt gataactttt cgcaagaaga
    36781 gcagtttgac ctctttcatc aggttgcgga agaaagtgtg aactttggcc ccagtccaga
    36841 agagagtttc cagcaggact atgacctaga tgttgattgg cttgatgttt gttttgatac
    36901 agtccagcat gtttttagtc atcgaaaatg gcatgtacag attgtagcag gtcaggtgag
    36961 tgacttccat gatttttcag atagggaagt tcgttggctt tcaccagaag aattcaagaa
    37021 ttacccactt gccaaacccc aacaaaaaat ctggcaggct tatgcacaag ccaacttaga
    37081 cagtagccaa gactagctat tgtgtcttct gactattttt tggtataata gtagagaaaa
    37141 aggtgaacat atgaaaaaaa tactaattgt agatgatgag aaaccaatct cggatattat
    37201 caagtttaat atgaccaagg aaggttatga agttgtaact gcttttaatg gtcgtgaagc
    37261 gctagagcaa tttgaagcag agcaaccaga tattattatt ctggatttga tgcttccaga
    37321 aattgatggt ttagaagttg ctaagaccat tcgtaagaca agcagtgtgc ccattcttat
    37381 gctttcagcc aaagatagtg aatttgataa ggttatcggt ttggaacttg gggcagatga
    37441 ctatgtaacg aaacccttct ccaatcgtga gttgcaggcg cgtgttaaag ctcttctgcg
    37501 tcgttctcaa cctatgccag tagatggtca ggaagcagat agtaaacctc aacctatcca
    37561 aattggggat ttagaaattg ttccagacgc ctacgtggct aaaaaatatg gcgaagaact
    37621 agacttaacc catcgtgaat ttgagctttt gtatcattta gcatcgcata caggtcaagt
    37681 catcacgcgc gaacacttgc ttgagactgt ctggggttat gactattttg gtgatgtccg
    37741 cacagttgat gtgactgtac gacgtctgcg tgagaagatt gaagatacgc ccagccgacc
    37801 agagtatatc ttgacgcgcc gtggtgtagg gtattacatg agaaataatg cttgatttac
    37861 tgaaacaaac catttttacc agagatttta tctttatcct gattttgtta ggtttcatcc
    37921 ttgttgtgac cctcttatta ctggaaaata gacgtgataa tattcagttg aagcaaatca
    37981 atcaaaaggt taaagatttg attgcaggag attattccaa gattcttgat atgcaaggtg
    38041 ggtctgaaat caccaatatt accaataatt tgaatgactt gtcggaggtt attcgtctca
    38101 ctcaggaaaa tctagaacaa gagagtaaga ggctaaatag tattctgttt tatatgacag
    38161 atggggttct tgcgactaac cgtcggggtc agattatcat gattaacgat acagccaaga
    38221 agcaactggg gttggttaag gaagatgttc tgaatagaag cattttggaa ttgctcaaga
    38281 tagaagaaaa ctatgaattg cgtgatttga ttacccaaag tccagaattg ttgctagatt
    38341 cccaagatat caatggcgaa tatttgaacc ttcgagttcg ctttgccttg atacgtcgag
    38401 agtctggctt tatttcaggt ttggtggctg ttttgcatga tacgacggag caggagaagg
    38461 aagaacgcga acgaagactc tttgtttcca atgttagcca tgagttacgg actcctctga
    38521 ctagcgtaaa atcctatctt gaagccttgg atgagggggc tttgtgtgaa actgtagcac
    38581 cagactttat caaggtttct cttgatgaga ccaaccgtat gatgcgcatg gtgacggatc
    38641 tcctccatct ttcacgtatt gataatgcta ccagtcacct agatgtggaa ctgattaact
    38701 tcactgcttt tattaccttt atcctcaatc gttttgacaa gatgaaagga caggaaaagg
    38761 agaaaaaata tgagctggtg agagattatc ccatcaattc tatctggatg gaaattgata
    38821 cagataagat gacgcaggtt gtcgacaata ttttaaataa tgctattaag tattcgccag
    38881 atgggggtaa aatcactgtc agaatgaaga caactgaaga ccagatgatt ttatccattt
    38941 ctgaccacgg tttggggatt cctaagcagg atttaccacg tatctttgac cgtttctatc
    39001 gtgtggatcg tgctagaagt cgtgcacaag gtggtacagg tctaggactg tctatcgcta
    39061 aagaaattat caaacaacat aagggcttta tttgggccaa gagtgaatac ggcaagggtt
    39121 caacctttac cattgtactc ccttatgata aggatgcagt gaaagaagaa gtatgggagg
    39181 atgaagtaga agactagaat gagtgaaata ggctttaaat acagtatttt agcgtcgggt
    39241 tccagtggaa attcttttta tctggaaacc tcaaaaaaga agcttttagt agatgcaggc
    39301 ttgtctggca agaaaattac cagtctgcta gctgaaatta accgtaagcc agaagacctg
    39361 gatgccatct tgattaccca tgagcattca gatcatatcc atggagtagg cgttttggct
    39421 cgcaagtatg gtatggatct ttatgccaat gaaaagacct ggcaagctat ggaaaatagt
    39481 aaatatcttg gcaaggtgga ttcttcgcaa aagcatattt ttgaaatggg taaaaccaaa
    39541 acctttggag atatcgacat cgagagtttt ggtgtaagcc atgatgcagt cgcaccgcag
    39601 ttctatcgct ttatgaagga tgataagagt tttgtcctct tgacagatac aggttatgtc
    39661 agtgaccgta tggcgggcat tgtcgaaaat gcggatggtt atcttatcga ggccaaccat
    39721 gatgtagaga ttttgcgatc aggttcttac gcttggcgac tcaaacaacg aatcctatct
    39781 gaccttggtc acctttctaa cgaggacggt gctgaagcta tgattcggac gctaggaaat
    39841 cgtactaaga agatttacct tgggcattta tctaaggaaa acaatatcaa ggaactggct
    39901 catatgacca tggtcaatca gctggctcaa gctgatctgg gagtcggagt agactttaag
    39961 gtttatgata cctcaccaga taccgcaaca ccattgacag agatatagaa agaacgctga
    40021 gaaggtgttc tttttatatt gactgaacac ctaaaaagta atacaatggt gttaccatta
    40081 aaaaagggag cacaaaagat gactactatt acattaaaag tttctgaagc tgataaaaca
    40141 tttatgaaag caatggctaa gtttgaagga gtttcccttt cggaacttat tcgcaccaaa
    40201 actcttgaag ccctagaaga tgaatacgat gctcgtgtgg cagatttagc ctatcaagag
    40261 tatttagaag acttggaaaa aggagttgaa cccattactt gggaagaaat gatgcatgat
    40321 ttaggcttga aggatgaata atttgtataa attagttcca acaagacgtt ttatcaagca
    40381 attgaaaaaa ttggaccgtt atacgcagaa gctaattaca aactatttac aaaccaatgt
    40441 tttggaagac ccaagacgac acggaaaggc tttggttggt aatcgcgttg gtcaatggcg
    40501 ctatagaatt ggtaattatc gagttatcgt acaaattgta gatgatgaat tagttgttgc
    40561 tactctagaa gttggtcatc ggagagatat ttattgaatt aaagctgagt aataacagta
    40621 tagaaagtaa ggatagtaaa aatttgaata aaaaatggtt atttgcagat tattatgata
    40681 taacaattat tttacttgct ttaatctctg ttattctggt attacttgga tttgctgaga
    40741 tgattgattt ggacaatccg ccttatagta ttattgattt agtaatctgg ggtgtttttg
    40801 taattgatta tagttggcgc ttttttatta ctaaaagaaa atggcgcttt attcttgaaa
    40861 atatttttga tttattgact attctccctt taaatgctat ttttacagtg tttcgattag
    40921 gacgtatctt ccgcttagca aggctgacaa aattactgaa actgactcgt ttacttagaa
    40981 taattggact tacaggtaaa ttagaaagaa aaattagtag attcttacga acaaatggat
    41041 tgatttatat cttgtatgtt aatatcttta ttgttctagt agggagttcg attttatccg
    41101 ttgttgaaga aaaatccttc tcagatagtc tttggtgggc tcttgtgaca gtaactactg
    41161 ttggttatgg tgatattgtt ccagtttccc tttttgggaa gacgaattat cgagccaaag
    41221 aatattaata gagaaacaat ccgagaagat agaagaactg catcggatga tacaagattt
    41281 gcttgaaaaa acataataaa aataaagatg agacaaagtt tgagttgagg agaaatcaag
    41341 taagttttga cacaatagaa cgcatattat caggtataaa aacttgatac tatgcgtttt
    41401 atagagaaat gaaataaaat ctgaacaaat caattaggaa tttaaagcat tttataacaa
    41461 cgttttagag taatgggggg ctatttcaac ttcaacctac tataatacag aaaaaaacaa
    41521 ctccctgata attcaaggag ttgtctatag ttaaattagt ttttagaagc ttcttggaat
    41581 tctgggtttt tccatgcttc gtcaatgata gcttgcaatt ctttagcaga tgcttgcatt
    41641 ttttgagttt ctgcgtcgtt caatgggata tttactggac gaacaatacc atgtgcacca
    41701 acaacagctg gttgaccgat aaagacgttc tcaactccgt attgaccttc ttggaatact
    41761 gaaagtggaa gtactgcatt ttcatcgtca aggattgctt tagtgatacg agcaagggct
    41821 actgcgatac cgtagtatgt tgcacctttt ttgttgatga ttgtgtaggc tgcatcacga
    41881 acaccttcga acaattcaat caattcagct tcttgaacat tttgagtatc tttaaggaat
    41941 tcttcaaggt ttacaccagc gatgttagcg tgtgaccaaa cagcgaactc agagtcaccg
    42001 tgttcaccca tgatgtaggc gtgaactgaa cgagcatcta cgtccaattt ttcagcaagt
    42061 gcttgacgga aacgagctga gtcaagtgaa gtacctgaac cgataacgcg ttctttaggg
    42121 aaaccagaga atttccaagt tgagtaagtc aaaacgtcaa ctgggttagc agcaacaagg
    42181 aagatacctt tgaaacctga ttcaacaact tgagttacga ttgatttgtt gatagcaagg
    42241 tttttaccta caaggtcaag acgagtttca cctggttttt gaggcgcacc tgcagtgatc
    42301 acaacaaggt cagcgtctgc acagtcagag tattgagctg catagatttt tttaggtgaa
    42361 gtgaaggcaa gggcgtgact aaggtcaagc gcatcaccaa cagctttttc atgcaattgt
    42421 ggaatttcga taattccaag ctcttgtgca attccttggt taacaagtgc aaaagcgtaa
    42481 gatgaaccta cagcaccatc accgacaagg ataacttttt tgtgttgttt agttgaagtc
    42541 attgttttaa acatctcctt aattttatta ggggattttc cctagacaac ttcattctat
    42601 cacttttaaa aaactttgtc acgaatatgc cttatagttc tcgatgtaaa cgttttagtg
    42661 gtttagaggc tgaaatagat gggaatttat ggtataatgt tgttacttac taattgtgaa
    42721 atgaggcatt tattaatgca ggataaaaat ttagtgaatg tcaatctgac aaaggagatg
    42781 aaggcaagtt ttatcgacta cgccatgagt gttatcgtag cgcgagctct tcctgatgtt
    42841 cgagatggct taaaacctgt tcaccgtcgc attctttacg gaatgaatga attgggtgtg
    42901 accccagaca aaccccataa aaaatctgct cgtattacag gggatgtcat gggtaaatat
    42961 cacccacacg gggattcctc tatttatgaa gccatggtcc gtatggctca atggtggagc
    43021 taccgttaca tgcttgtaga tggtcatggg aattttggtt ccatggatgg agatagtgct
    43081 gccgctcaac gttataccga ggcacgtatg agcaagattg ctctggaaat gcttcgtgat
    43141 atcaacaaaa atacagttga tttcgttgat aactatgatg ccaatgaacg ggaacccttg
    43201 gtcttgccag cgcgttttcc aaaccttttg gttaatggag caactggtat cgcggttggg
    43261 atggcaacca atattccacc tcataatctg ggtgaaacca ttgatgcagt gaagttggtc
    43321 atggataatc ctgaagtgac taccaaggac ttgatggaag tcttgcctgg accagatttt
    43381 ccaactggtg ctcttgtcat ggggaaatca ggtatccata aggcttatga aacaggtaaa
    43441 ggttcgattg tcctacgttc tcgtacagag attgaaacga ctaagactgg tcgtgagcgc
    43501 atcgttgtaa cagaatttcc ttacatggtc aataaaacca aggtgcatga gcatattgtt
    43561 cgcttggttc aggaaaaacg cattgagggt atcacagcag tacgtgatga gtcaaaccgt
    43621 gaaggtgttc gatttgttat tgaagtcaag cgcgacgcct cagccaatgt tattctcaat
    43681 aacctcttca aaatgaccca aatgcaaacc aattttggtt tcaatatgct cgctatccaa
    43741 aatggtatac cgaaaatttt gtctcttcgt cagattttgg atgcttatat cgagcaccaa
    43801 aaagaagtgg ttgttcgtcg tacacgtttt gataaggaaa aagcggaagc gcgcgctcat
    43861 atcttagaag gtctcttgat tgcgctagac catatcgacg aagtgattcg tatcatccgt
    43921 gctagtgaaa cggatgcgga agctcaagct gagttgatga gcaagtttaa gctttctgaa
    43981 cgtcaaagtc aagctatcct tgatatgcgt cttcgtcgtt tgacaggttt ggaacgcgat
    44041 aagattcaat ctgagtatga tgacctcttg gctctgattg cggatttagc agatattctt
    44101 gctaagcctg aacgtgtttc tcaaattatc aaagacgaat tggatgaagt taaacgtaaa
    44161 ttttctgata agcgccgtac agagttgatg attggacagg tcttgagtct cgaggatgag
    44221 gacttgattg aagaatcgga tgtcttgatt accctttcta acagaggcta cattaagcgt
    44281 ttggatcagg acgagttcac tgctcaaaaa cgtgggggtc gtggtgtcca aggaacggga
    44341 gtgaaagatg atgactttgt tcgtgagtta gtgtcaacta gcacccatga tcatctgctc
    44401 ttcttcacaa acaagggacg tgtctatcgt cttaaaggtt atgaaattcc tgagtatggt
    44461 cggactgcca aagggctacc agtagtcaat ctcttgaaat tggatgaaga cgaaagtatt
    44521 cagacggtta tcaatgttga gtctgatcgc agtgatgatg cttatctctt ctttacaacc
    44581 cgtcacggta ttgtgaagag aactagtgtt aaggagtttg ccaatattcg tcaaaatggt
    44641 ctcaaagcgc tgaatttaaa ggatgaagat gagttaatca atgtcttgtt ggcagaagga
    44701 gatatggata ttatcattgg taccaagttt ggttatgcag ttcgctttaa tcaatcagcc
    44761 gttcgtggta tgagtcgtat cgccactggt gtgaaaggtg ttaaccttcg tgaaggagac
    44821 acagttgttg gtgccagctt gattactgat caagatgagg ttcttattat cacagaaaaa
    44881 ggatatggta agcgtacagt cgctactgaa tacccaacaa aaggtcgtgg tggtaaggga
    44941 atgcagacag ctaaaattac cgaaaaaaat ggcttgctgg ccggtcttat gactgttcaa
    45001 ggggatgagg atttgatgat tatcactgat acaggtgtca tgattcgaac caatcttgcc
    45061 aatatttcac aaacaggacg tgcaactatg ggagttaaag taatgcgcct ggatcaagat
    45121 gctcagatag tgactttcac aacggttgcg gtggcagaaa aagaagaagt tgggacagaa
    45181 aacgaaacag aaggtgaagc ataatgtctc aaaaaaataa taaaaagaaa aacaagcgaa
    45241 aaaatctgct gacaaatatc ctagcaggat ttctgatatt actgtcactg gctttgattt
    45301 ttaatactca aattcgaaat attttcatag tctggaatac caataagtat caagttagcc
    45361 aggtatcaaa agaaaaatta gaagaaaatc aggatacaga aggcaatttt gactttgatt
    45421 ctgtcaaagc tatctcttcg gaagctgttc taacttctca atgggatgct caaaaattac
    45481 cagttattgg gggaattgca attcctgaat tggaaatgaa tttgccgatt tttaaaggac
    45541 ttgataatgt taatctcttc tacggagctg gtacaatgaa acgcgagcaa gtaatgggag
    45601 aaggaaatta tagtctagct agtcaccata tctttggtgt tgataatgct aataaaatgt
    45661 tattttctcc tttagataat gctaaaaatg gcatgaagat ttatctaact gataaaaata
    45721 aagtttatac ttatgaaata cgtgaagtca aacgtgtgac accagatcgt gttgatgaag
    45781 ttgatgatag agatggggtc aatgaaatca cattagtaac ctgtgaagac cttgctgcta
    45841 cagaacgtat tattgtcaaa ggtgatttga aagaaacaaa agattattca caaacatctg
    45901 atgaaatcct aacagctttc aatcaaccat ataaacaatt ttattaatac aaatcagtga
    45961 aatcatggat ttcactgatt ttttaagatt ttcataaaaa tatagtaaaa tgaaataaga
    46021 ataggactaa tcgttcagga cagtcaaatc gatttctaac aatgttttag aagtagaggt
    46081 gtactattct agtttcaatc tactataaac ttttatgaaa tacagaaaac gcttcctaaa
    46141 acctaaagtt tgtgatataa ttatcaagaa aaaagattta ggagtttatt atgatttctt
    46201 cagaatttat ctcaaagatt gaatttgctt gcaataagaa agaaagtctt tatagtcaaa
    46261 gcaaatttaa gtatgcgatt cgttcgatat tcgcaggtgc atttttaacc ttcagtactg
    46321 ctgcaggtgc agttggggct gacttgatta ataaaattgc accaggtagt ggacgcttcc
    46381 tctttccatt cgtttttgct tggggcttgg cctacattgt ttttttgaat gccgagttgg
    46441 tcacttcaaa catgatgttc ttgactgctg gtagtttctt aaaaaaaatc tcttggagaa
    46501 aaacagctga gattttacta tactgtacct tgttcaacct tatcggatcc ttgatagcag
    46561 ggtggggctt tgctcattcg gcagcctatg cgaatctgac acacgatagt ttcatctcag
    46621 gtgttgttga gatgaagtta ggccgctcaa atgaattggt cttgcttgag gcgattttgg
    46681 caaatatttt cgtaaatatt gcgattctgt catttatttt ggtcaaagat ggtggtgcca
    46741 aactttggct tgtgttgtca gctatttaca tgtttgtatt cttaacaaac gagcacattg
    46801 cggcgaactt tgcttctttc gcgattgtga aattcagtgt tgctgcggat tcaattgcca
    46861 acttcggtgt tggaaatatg cttcgccact ggggtgtgac tttcatcgga aactttatcg
    46921 gaggaggcct cttgatgggt cttccatatg ccttcctcaa taaaaacgaa gatacttatg
    46981 tagattaaga aaatgagcac gattgagtcg tgcttttttc attttcaaaa taaggtaata
    47041 gctatttctt atatcaaaat atagaaaact gatatttgta aactataact caaggtgcta
    47101 caatatcctt aataaaataa tatggaggtc accttatgac tcgtgatttt aaatttgaaa
    47161 ctctacaact acatgctggt caagttgtga ctccagctac tgagtctcgt gcagtgccga
    47221 tttatcaaac aacatctttt gtttttgatg acacgtagga aggtgctgat ctgtttgcct
    47281 tgaggaaacc agggaacatt tatactcgta tcaccaatcc tacaacagct gcccttgaag
    47341 gtggtgttga agcgctagca acagcatcag gtatgactgc agtgacttat acgattttgg
    47401 cgattgccca tgctggtgac catgtagtgg ctgcttcgac tatttacggt ggaaccttca
    47461 atcttttgaa agaacccctt cctcgttatg gtatcacaac aacctttttc gatattgata
    47521 atttggagga agtagaagca gctatcaaag acaataccaa gcttgtcttg attgaaacct
    47581 tgggtaaccc cttgattaat attccagacc tggaaaaact ggcagagatt gctcataaac
    47641 atcaaatccc acttgtgtca gacaatactt ttgcaacacc ttatttgatt aacgtcttct
    47701 ctcatggcgt tgacattgcc attcactctg tgactaagtt tatcggtggg catggtacaa
    47761 ctattggagg aataattgtc gatagtggtc gttttgactg gacggcttca gggaaattcc
    47821 ctcaatttgt tgacgagggt ccaagctgcc acaatttgag ctatactcgt gatgtgggtg
    47881 cagcagcctt tattatagct gttcgagttc aattgcttcg tgatacaggt gcagccttgt
    47941 caccattcaa tgctttcctc ttgctacaaa gacttgaaac ctcttcactt cgtgtggaac
    48001 gccatgtaca aaatgctgag acaattgttg attttcttgt caaccatcct aaggtagaaa
    48061 aggtaaatta tccaaaactt gcagatagtc cttatcatgc cttggctgag aaatacttgc
    48121 caaaaggtgt cggttcaatc tttaccttcc acgtcaaagg tggcgaggaa gaagcacgca
    48181 aggtcattga taatttagaa atcttttctg accttgcaaa cgcggcagat gctaaatcgc
    48241 ttgttgtcca tccagcaaca accactcacg gtcaattgtc agaaaaagac ctagaagcag
    48301 caggtgtcac accaaactaa attcgtttgt caatcggtct tgaaaatgta gaagatttga
    48361 ttgaagactt gcgcttggcc ttggaaaaaa tttaaagtaa aagaagataa acagtgggct
    48421 tcgactcact gtttttgatt ttccctcagg catgatataa tggttacaga agtctagaaa
    48481 gaggaacgat atgaacgaaa tcaaatgtcc caactgtggg gaagtcttta cagtaaatga
    48541 gagtcagtat gccgaactct tgtcccaagt gagaacggca gagtttgata aggaactaca
    48601 cgataggatg aagcaggaac tggccttggc tgagcaaaag gccatgaatg agcaacagac
    48661 taaactggct cagaaggatc aagaaattgc gcaattacag agtcagatcc aaaactttga
    48721 tacagaaaaa gaattggcca agaaagaggt tgaacagaca agccatgagg ctctcttggc
    48781 taaggacaag gaagtacagc tcttagaaaa tcagttggct accttgcgtt tggagcatga
    48841 aaatcaacta caaaagaccc tttctgacct agaaaaagaa cgggatcagg ttaaaaacca
    48901 actacttttg caggaaaagg aaaatgaatt atctttggct tctgttaagc aaaactacga
    48961 agcccagctc aaggcagcta gtgaacaagt cgagttttat aagaatttta aggctcaaca
    49021 atctacaaaa gcgattgggg aaagcctaga acagtatgca gagagtgagt ttaacaaggt
    49081 tcgtagtttc gcctttccaa atgcttactt tgagaaggat aacaaggtct cttcgcgtgg
    49141 gtctaaaggg gactttatct tccgtgagtg tgatgaaaat ggagttgaaa tcatttctat
    49201 catgtttgag atgaaaaacg aagcggacgg aacagagaag aagcacaaga atgcagattt
    49261 ttacaaggaa ttggacaagg accgtcggga gaagaactgt gagtatgccg ttttggtgac
    49321 catgcttgag gctgataatg actactttaa cacagggatt gttgacgtca gtcacgagta
    49381 tgaaaaaatg tatgttgttc gtcctcaatt ctttatccaa ttgattggtc tcttacgtaa
    49441 tgcggcgcta aattccctaa aatacaagca ggagttggcc ttggttcgcg agcaaaatat
    49501 tgacattacg cattttgagg aagatttgga tgcctttaag ctagcttttg ctaagaacta
    49561 taattcagct tcgactaact ttggaaaagc tattgatgaa atcgacaagg ccatcaaacg
    49621 catggaagag gttaagaaat tcctgaccac atctgaaaac caactccgtt tagctaacaa
    49681 caaattggaa gatgtctctg ttaaaaaatt gacccggaaa aatccaacaa tgaaagcgaa
    49741 gttcgaagca ctgaaggggg agtagaaagc aaaaatgaac ggtattatta acttaaaaaa
    49801 ggaagcagga atgacctcgc atgatgcggt ttttaaactg cgtaagattt tgggaaccaa
    49861 gaaaattggt catggtggaa ccttggatcc ggatgtggtg ggtgttttgc cgattgcggt
    49921 tggcaaggcg acacgcatgg tcgagtttat gcaggacgag ggtaagatct atgaggggga
    49981 aatcactctg ggctattcca cgaagactga ggatgctagt ggggaagtgg tcgcagaaac
    50041 ccctgttttg tctctcttgg atgaaaagct tgttgatgaa gcgattgcta gcttgactgg
    50101 gcctattact cagattcccc ctatgtattc ggcagttaag gttaatggtc gcaagctcta
    50161 tgagtatgcg cgtgctggtc aggaagtgga gcgtccagaa cgtcaggtga ccatttatca
    50221 atttgagcga acaagtccga tttcttatga tggccaactt gcccgattca cttttcgtgt
    50281 aaaatgcagt aaagggacgt acatccgtac tttgtcagtt gatttgggtg aaaagcttgg
    50341 ttatgcggct catatgtccc atttgactcg tactagtgct gctggcttac aattagaaga
    50401 cgctcttgcc ttggaggaaa ttgctgaaaa agtagaggct gggcaattag attttctcca
    50461 tcctttagag attgggacag gtgaccttgt caaagttttc ctaagtccag aagaggctac
    50521 agaagttcgc tttggtcgtt ttattgagct agaccaaacg gacaaagaac tggctgcctt
    50581 tgaagatgat aaattgttag ccattctaga aaaacggggc aatctctata agccaaggaa
    50641 ggtttttagc tagatcgttt aggaataaaa atcgggtgat agataacaat tgcttgataa
    50701 aaccccatac taatagtaga atggttttgg gaattataat attccaattg ttgcgagttg
    50761 taggtactca aataatctat atagaaattt agaggtgtga aatgaagcaa tttaaaattc
    50821 tttcagataa atatttagag tccattacag gttctgatgg gaacttaggc ccaggatttg
    50881 gtgtgataat tccatgatgc gaaatgagtt tcgagaaagg gtggagcaac ttcttcaaca
    50941 aaaagaaata aatgaaaata gtgagttgag tcacctgttt cgtcttgcta tacaaaattt
    51001 agacagaaat gaaaaatacc aatcggtcat ggccaatttg agtcaagggt tgtcacttta
    51061 cctcatgacg catcattacc aggcacctaa gtctgtcatt gattttggtt tatggattgc
    51121 caaagctcct agccaggaaa gagggagact ggctttcttg cagatacttg ctcaaaccct
    51181 acagggcttt cgttaaaact gtcgaacatc agagggaaga ctctggtgtt ttttgctgat
    51241 gagactataa attttgtaaa aaatgtgata gaatagacga cggataaaat aacggaggat
    51301 agcatgcaaa atagaccaat cattattgga gtgacaggtg gttctggtgg tggtaaaacc
    51361 agtgtttcaa gagccatttt atcgcatttt cctgatgaaa agatttccat gattgagcat
    51421 gattcatact acaaggatca gtctcatttg acctttgaag agcgtgtcaa aaccaactat
    51481 gaccatcctt ttgcctttga tacagacttg atgatcgagc agattaagga attgttggca
    51541 gggcgtccgg tggacatccc gacctacgac tatacagagc atacacggag tagcaagacc
    51601 tatcgtcagg agcctcaaga tgtctttatc gttgagggca ttttggtctt ggaggacaag
    51661 cgtctgcgcg atttgatgga tatcaagatt tttgtggata cggatgacga tgtgcgcatt
    51721 attcgtcgta tcaagcgtga tatggaggag cgtggccgta gccttgatag cgttattaac
    51781 cagtacttag gtgtggtcaa accaatgtac caccagttta tcgagtcaac taagcgttat
    51841 gctgatatcg tcattcctga aggggttagc aataccgtgg ctatcgacct gttgacgacc
    51901 aagattgcaa agattttgga agaagctcga aacagcaaat aatcacatgt ggaggccttg
    51961 cctccttttt ctatttttcc ctcataatgg tacataaatg gagattttta ggcatatttt
    52021 tggtataata atacccatga aagagcaagt aaatgaatag taggtggaga tggaaaagta
    52081 tttatcggta acaactttga ccaagtatct gaaaatgaaa ttcgataaag acccatactt
    52141 ggaacgggtc tatttaactg gtcaagtttc caactttcgt aaacgaccta ctcaccaata
    52201 tttctcccta aaggatgacc atgcagttat tcaagtgacc atctggtctg ggatttatca
    52261 gaaattaggg tttgacctgg aagaaggaat gaagatcaat gtgattgggc gtgtacaggt
    52321 ctatgaacca agtggtagct actccatcat cattgaaaaa gctgagcctg atggggttgg
    52381 ggcgcttgcg attcagtttg aacaacttaa gaaaaaattg acagaagaag gcctgtttca
    52441 agaacgcttc aagcaagctc taccccaatt ttctaagaga attggggtag taaccagccg
    52501 tagtggagcc gttattcgag atattatcac gaccgtcagc aggcgattcc caggtgttga
    52561 catccttctt tatccgacca aggttcaagg tgaaggggct gcggaggaaa ttgctagaaa
    52621 tattgcgcgt gctaatcaat gggacgattt ggatttgctc attattggtc gtggaggtgg
    52681 ttctattgag gatctctggg cctttaacga agaaattgtg gtacgagcta tttttgaatc
    52741 tcgtttgcca gttatctcta gtgtggggca tgagacggat gtgaccttgg cagattttgt
    52801 ggcagatcga cgcgctgcaa cgccaacagc ggcggctgaa ctggcaacac ctgtgaccaa
    52861 gttggatgta ttagctcatt tgcaaaatca ggaaaaacgg atggcaacgg cagtccgaaa
    52921 tgttctatct aagaaacaag aggctttgaa aaaatgcagt cagtctgtta tctttagaca
    52981 acctgagcgc ttgtatgacg gttatttgca acgcttggac caactgcaac tgcgtttgaa
    53041 acaaagtttg cgaactcgga tttctgataa caaacaatta gttcaagcaa gaactcatca
    53101 attagtacaa ttatcacctg ttaccaaaat ccaacgctat caagaccgtt taggacagtt
    53161 ggacaagctc ttaggtagcc aaatggcgtt agtttatgac gccaaggttg ctgaggccaa
    53221 gcgactttcg gaagctttgc tcatgttgga tactagccga atcgtggcgc gtggttatgc
    53281 tattgtcaaa aaagaagagt ccgttgtaga ttcggttgag agtttgaaga aaaaagacca
    53341 agtaacgctt ttgatgcgag atggtcaagt agaattagag gttaaagatg tcaaaacaaa
    53401 agaaatttga ggaaaatcta gcagaactgg aaaccattgt ccaaagtttg gaaaatggtg
    53461 aaattgctct ggaagatgcg attactgcct ttcaaaaggg catggtcttg tcaaaagagc
    53521 tccaagctac gctggacaag gctgaaaaga ccttggtcaa ggtcatgcaa gaagacggaa
    53581 cagaaagtga ttttgaatga aaaagcaaga aaaattagct cttgtcgagt cggctttgga
    53641 agatttttat ggagaccagc agtttgcctc tagtttacgg gagtctgttc tctattctat
    53701 tcatgctggt ggcaagcgta ttcggccttt tctcttgtta gaagttctgg aagccttgca
    53761 ggttgccatc aaacctgctc acgcgcaggt agctactgcc ttggagatga ttcatacagg
    53821 gagcttgatt cacgatgacc ttcctgctat ggatgatgac gattatcgaa gagggcggtt
    53881 aaccaatcac aagaaattcg gtgaagctat ggccattttg gctggagatg ccttattctt
    53941 agacccatat gccttgattg cgcaggcaga tttgccaagt cagatcaagg tggacttgat
    54001 tgccaactta tcccttgctt caggtagtct gggtatggtg gcagggcaag ttttggatat
    54061 ggagggcgaa caccagcact tgtctctgga agaacttcag actattcatg ccaataagac
    54121 tgggaagtta ctagcctatc ccttccaagc ggcagctatt atagctgaat tgtcacctga
    54181 aatgcaggtg aagctgaaaa ctgtgggtga attgattgga cttgcttttc aagtcagaga
    54241 tgatgtactg gatgtgacag ctagttttga ggaaatcggc aagacacctc aaaaggatct
    54301 gcaggcagaa aaatcaacct atcctgcctt gttgggcttg gaagagtcca ttgccttttg
    54361 taaccaaacc ctggatcaag ctaatgaaaa attagaagaa attgcccagc agcttccctt
    54421 tgaaacagaa tcgattgtaa gtgtagtaga aagtttgaga atcaatggct aaggaaagag
    54481 tggatgtact agcttataaa caggggttgt ttgaaacgag agagcaggcc aagcgaggtg
    54541 tgatggctgg cctagtcgta gcagtcctta atggagaacg gtttgacaag ccaggagaga
    54601 aaattccaga tgacaccgaa ttaaaactca agggggagaa actcaagtat gtcagccgtg
    54661 gtggtttgaa actggaaaag gccttgcagg tctttgattt gtcggtggat ggcgcgacta
    54721 cgattgatat cggggcctct actggaggtt ttaccgatgt catgctacag aatagtgcca
    54781 agttggtctt tgcagtcgat gttgggacca atcagttggc ttggaaatta cgccaagacc
    54841 cacgagttgt cagcatggag cagttcaatt tccgctatgc tgaaaagact gatttcgagc
    54901 aggagccgag ctttgccagt attgatgtga gtttcatttc ccttagtctg attttgccag
    54961 ccttgcaccg tgtcttggct gatcaaggtc aggtggtagc acttgtcaaa cctcagtttg
    55021 aggcaggacg tgagcagatt gggaaaaatg gaattattcg agatgttaag attcatcaga
    55081 atgtccttga atctgtaaca gctatggcag tagaggcagg tttttcagtc cttggcttgg
    55141 acttttctcc catccaaggt ggacatggaa atattgaatt tttagcgtat ttgaaaaaag
    55201 aaaagtcagc aagcaatcag attcttgctg agattaaaga agcagtagag agggcgcata
    55261 gtcaatttaa aaatgaataa aaaagaaaga cttgaaaaaa ttagaagatt ggtgacagat
    55321 tatcaaatcg gcacgcaaga agaaattgta gaacatttga aagaagcagg tatcactgcc
    55381 actcaggcga cggtatcccg agatatcaaa gagttaggta ttgtcaaaat tcctttgaga
    55441 gacaacacct atgtctacga attaccaaaa tcaatcgtaa aaagtctgca actggctgaa
    55501 gacaatatcg aatcggctga attgatggat aagatgatca atctccaagt tattccagga
    55561 aatacggctt ttgtaaaagc tcagttaatc gagacttttg cagacaagat ttttagctgt
    55621 ttgactgatg atagctcgat tttagtcatt gccagaagtg gaagtctagc agaggaaatc
    55681 tttgaacaag taaaaaattg gtaggtctat atgttacttg aaatttcgat aaaaaacttt
    55741 gccattattg aggctatttc cctcaatttt gaaaagggga tgactgtcct gactggtgaa
    55801 acgggtgcag ggaagtcgat tatcattgat gccatgaata tgatgttggg agctcgtgcg
    55861 acgacagatg ttattcgtca tggtgtacca aaggcagaga ttgaggggct tttctcagtt
    55921 gagaatagtc gtcttttaca ggaaattttt gatgagcaag gtttagaatt aggtgatgaa
    55981 attatcatcc gtcgggaaat cttgcaaaat ggtcgtagta tcagccgtgt aaatggtcag
    56041 atggttaatc tgtctgtttt gcgagctatt gggcaacatc ttgtagatat tcatggtcag
    56101 catgaccatg aggagttaat gcgtccccaa ctgcatatcc agatgttgga tgaatttggt
    56161 gatgccgctt tttgggactt gaaagaaacc tatcaaacga gttttgatgc ctatcggaaa
    56221 atgcgcaagc aggttctgga agtcaagaaa aaccaacagg aacacaaggc acgtatcgaa
    56281 atgttggaat ttcaaatggc agagattgag gcagcaaact tgcaggctgg agaagacttg
    56341 gctctcaatc aagagcgaga taaactcctc aaccacaaaa atattgcgga tacactgacc
    56401 aatgcctaca gtatgttgga caatgaagat ttttcaagtc tggccaatgt tcgttcagcc
    56461 atgaatgaca tggaaagtgt cgaagagtat gaccctgaat accgtgaaat ttcaagctct
    56521 ctgtctgaga cctactatgt tttagaagac attagcaaac gtttggaagc tatcattgag
    56581 gaccttgatt ttgatggcaa tcgcctcatg caggttgaga atcgtttgca cctccttcat
    56641 actattactc gtaagtatgg tgggactgtt gatgatgttt tgctttattt tgctaagatt
    56701 acggaagaat acaatctctt gacaggcaat aatctttcgt ctgaggacat ggaagcagag
    56761 cttaagaagt tggaagtcaa tcttgtcaat ttggcaggtc aacttgcttc tgcccgtcat
    56821 aatttggctc agcaactcga agctgagatt aaacaagaac tgcaagatct ttatatggaa
    56881 aaagcccagt ttcaggttcg ttttagtaag ggaaaattca gtcgtgaggg aaatgaaatg
    56941 gttgagtttt atatttcaac caaccctgga gaagacttta aacccttggt taaggttgct
    57001 tctggagggg aattatctcg tctcatgtta gccattaagt ctgccttttc acgtaaagaa
    57061 ggcaagacta gcattgtctt tgatgaggtg gatacgggag tttcaggtcg tgttgctcaa
    57121 gctattgctc agaaaattca taaaattggt cagcatggtc aggttttggc tatctcccat
    57181 ttgccacaag taattgcgat tgcagattat caattcttta ttgagaagat tagtaatgac
    57241 cattcaacgg tttcgactgt tcgtcttttg acggtcgaag agcgagtgga ggaagttgcc
    57301 aagatgttgg caggtgatga tgtgacagaa gcagccctga cgcaagccag agaattgttg
    57361 agaaacaggg agaaataaga tgacagacta ttatgtaatt ggagatgttc acggaaaagc
    57421 tgggatgctg gaagaccttc tcaaaacctg ggatggtcag gcccagttgc tctttctagg
    57481 tgatttgatt gacagaggtg aggatagtca ccgtgttctt gaaatggtca aggacttggt
    57541 ggacaatcaa ggggctatct gtttgtccgg aaaccacgag tatatgtttt tgacttggct
    57601 agatgaccca gaagaaagtt atgaccatta tcgtcgcaat ggtggagata caaccattaa
    57661 ctctatccta ggtcgtccct tggatgcacc agttgatgga gtagaagatg ccaagcgggt
    57721 tgccgctgaa gcggcagact tggtcgagtt cattcgtcaa atgccatttg tagtagagac
    57781 agacaagtat atctttgttc acgcaggtat tgatttgacc ttggatgact ggcatgaaac
    57841 aacagattat aaaaaagtct ggctcagaaa accattccac ggagctgaaa atcatactgg
    57901 aaaaaccatt gtcttcggtc atacaccggt ctatggtttg ttacagcaag aacgcggtac
    57961 atctgagctt tggactacag aagatggcaa gattggaatg gatggaggag ctgtttatgg
    58021 tggtgtcctt cacgggattg tctttacaga ccaaggaatg acagaacacc actttatcga
    58081 aaatgacggc tttgtcgctg aagattagta ctcctagcag ggtatggtct tgtcaaaata
    58141 tcaaaaacaa tttataataa atagataccc tgaaaggaag agaatcatga acttagaaga
    58201 attgaaaaaa cgacaggaga agatccgtaa cttctctatt atcgcccata ttgaccatgg
    58261 aaaatcaact ctagcagacc gcattttgga aaaaacagag acggtttcaa gtcgtgaaat
    58321 gcaggcccag cttttggata gcatggagct agagcgggaa cgtggaatta ctattaagtt
    58381 gaatgccatc gagttgaatt acactgcaaa agatggggaa acttatattt tccatttgat
    58441 tgacacacca gggcacgttg actttaccta tgaagtttca cgttcgctag ctgcctgtga
    58501 gggtgctatt ttggtggtcg atgcggctca aggaattgag gctcaaactc ttgccaacgt
    58561 ttatctggct ttggataatg atttggaaat catgcctatc attaataaaa ttgacctgcc
    58621 ggctgcagat ccggagcgcg tgcgtacaga gattgaagat gtgattggtt tggatgccag
    58681 tgaagcagtt ttggcttctg ccaaggctgg tattgggatt gaagaaatcc tcgagcaaat
    58741 tgtagaaaaa gtaccagcac caacgggtga tgtgacggcg ccacttaagg ccttgatttt
    58801 cgactctgtt tacgatgctt accgtggggt tatcctccaa gtgcgtgtca tggacggagt
    58861 ggtcaaacct ggtgataaga ttcagctcat gagcaatagt aagacctttg atgtggccga
    58921 agtcggtatt tttacaccaa aagcggttgg tcgtgatttc cttgcgactg gtgatgttgg
    58981 ttacattgcg gcgtctatca agacggttca ggatactcgt gtgggtgata ccgttacctt
    59041 ggcaaccaat cctgcggcag aaccattaca tggttataag cagatgaatc ctatggtctt
    59101 tgcgggtctc tacccaatcg aatcaaacaa atacaatgac ctgcgtgaag cgcttgaaaa
    59161 attgcaactg aatgatgcta gtcttcagtt tgaaccagaa acatctcagg cacttggatt
    59221 tggtttccgt tgtggatttc ttggacttct ccatatggat gttatccagg aacgtttaga
    59281 gcgtgagttc aatattgacc tcatcatgac agctccgtct gttatttaca aagttaattt
    59341 gaccgacggt gagtctatgg atgtgtctaa cccatctgag tttccagacc caactaagat
    59401 tgcgaccatt gaagagcctt atgtcaaggc gcaaatcatg gtaccacagg agttcgtcgg
    59461 agcagtaatg gagctagctc agcgtaagcg tggggacttt gtgactatgg actatattga
    59521 tgataaccgt gtcaatgtta tctatcaaat tcctcttgct gaaattgtct ttgacttctt
    59581 tgataaactt aagtcttcga cacgtggtta tgcaagcttt gactacgaat tgtcagaata
    59641 tcgcccatct aagctggtga aaatggatat tcttctcaat ggagataagg tggatgccct
    59701 cagctttatc gttcacaagg actttgccta cgaacgtggg aaactcatcg ttgataaact
    59761 caagaaaatc atccctcgcc aacaatttga agttccaatc caagcggcta ttggacacaa
    59821 gattgtcgct cgtactgata tcaaggccct tcgtaagaac gtacttgcta aatgttatgg
    59881 tggtgacgtt tctcgtaagc gtaaactcct tgaaaaacaa aaagctggta agaaacgcat
    59941 gaaatccatc ggatcagttg aagttccgca agaagccttc ctcagcgtct tgagtatgga
    60001 tgaagaataa gttatcagaa atgcctttcc ttgttcacag gaaatttata taagaatcaa
    60061 tagtaaagca ggcttagatg gtctgctttt ttagttttta tactcttcga aaatctcttc
    60121 aaaccacgtc agcgtcgcct tactgtatgt atggttactg acttcgtcag tttcatctac
    60181 aacctcaaaa ctgtgttttg agtaacctgc ggctagcttc ctagtttgct ctttgatttt
    60241 cattgagtat tagaacagaa aaatgcttgg agtatttgtt tgtgtgttta tttttatata
    60301 acaaactata aacaaaacaa aaatataaaa aaagagacaa aaaagaatag aaagtaatta
    60361 acaacgattt catatagtgt tatacttata gcataaagtt aatggaaaga agggaaaacc
    60421 ttatggtatt agattatttc tttgacaaaa accttgtgtt ttgcttagaa gcggataatc
    60481 aagaacaact ctttgatcag gtagcaagct tgttggaaga acgagaaatt gtcactccaa
    60541 cttatcgtga agccttgatc acgcgtgaaa agtcatttcc aactggttta gatatggaat
    60601 ttctaggaaa ggacttgcca aatgtagcga ttcctcatac agatattgtt cataatctag
    60661 ctgagaaagt ggtggttgtt cgattagaaa aaccagtaac ttttcacaat atgatagctt
    60721 ctgataagga agtagaagta tccctactct tctttatcat taacaattca agttcaagtc
    60781 aaacaaatat tctggctcag ttgatggact ttttcacagg aaatggacat cttgaagacc
    60841 tatcaaaaat ttccgaacca gaaaaacttt atgcttacat tgctgaagca accgcttaat
    60901 cttgtctatt aaaaaataaa atcggaggaa atccaaatga ttaaaattct tgctgcctgc
    60961 ggtgcaggtg ttaactcaag tcaccaaatt aaaagtgctc tagaagaaga actttcaaac
    61021 cgtggttacg atgttcactg tgatgcagtt atggtgaaag atgtaaacga agaccttatg
    61081 aaaggttacg atatctttac accaatcgct gcaacagacc ttggttttga accaggtatc
    61141 ccagttatcg aagctggccc aatcttattc tgtatcccag caatgagcgt tccagtattt
    61201 gacaatatta tacttcctgc gaaacaaaat atggtatagt agttccatga attatgaagc
    61261 aagtaaacaa ctaactgatg cacgatttaa atgtcttgtt ggtgttcagc gcaccacttt
    61321 taaagagatg ttagctgtat taaaaactgc ttatcaactt aaacacacaa aagggggacg
    61381 aaaacctaaa ttaagcctag aagaccttct tatggccact cttcaatatg tgcgagaata
    61441 ccgcacttat gaagaaattg cggctgattt tggtatccac ggattttggt atccacgaaa
    61501 gaaacttact ccgtcggagc caatgggttg aagtaactct tgttcaaagt ggttttacga
    61561 tttcaagaac tcctctcagt tctgaggaca cggtaatgat tgatgcgacg gaagtacaaa
    61621 tcaatcgccc taaaaaaaga attagcgaat
//
LOCUS       DAVDWT010000009        58182 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVDWT010000009 DAVDWT010000000
VERSION     DAVDWT010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 58182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58182
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.009"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            371..598
                     /locus_tag="VUW50_000673"
     CDS             371..598
                     /locus_tag="VUW50_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982617.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            886..3783
                     /locus_tag="VUW50_000674"
     CDS             886..3783
                     /locus_tag="VUW50_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HET7982618.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGQQFYGNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPKGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAVLTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDKVVISNLRDNPEAKD
                     PAAPSPLNIDMVLVQDPTTKRIFSIYDMFPEGRAVFGMPKTPEKAYEKIGDKTYQILY
                     KQGESGHYTVRENGEVYNAQNQKTDYRVVVNPTEPGYRDKGNLYKGQELIGNIYFAHS
                     TKNPFRVANTSYLWMSYSDDDGKTWSAPRDITPGLRKDWMKFLGTGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NERAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWDFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
     gene            4797..5249
                     /locus_tag="VUW50_000675"
     CDS             4797..5249
                     /locus_tag="VUW50_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET7982619.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            5268..6605
                     /locus_tag="VUW50_000676"
     CDS             5268..6605
                     /locus_tag="VUW50_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET7982620.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            6684..7568
                     /locus_tag="VUW50_000677"
     CDS             6684..7568
                     /locus_tag="VUW50_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7982621.1"
                     /translation="MVKERNLTCWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTIFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            7568..8401
                     /locus_tag="VUW50_000678"
     CDS             7568..8401
                     /locus_tag="VUW50_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7982622.1"
                     /translation="MKKKSGIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            8419..10512
                     /gene="nanB"
                     /locus_tag="VUW50_000679"
     CDS             8419..10512
                     /gene="nanB"
                     /locus_tag="VUW50_000679"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HET7982623.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            10524..11627
                     /locus_tag="VUW50_000680"
     CDS             10524..11627
                     /locus_tag="VUW50_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7982624.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            11801..12499
                     /locus_tag="VUW50_000681"
     CDS             11801..12499
                     /locus_tag="VUW50_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET7982625.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            12518..14035
                     /locus_tag="VUW50_000682"
     CDS             12518..14035
                     /locus_tag="VUW50_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET7982626.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            14160..15488
                     /locus_tag="VUW50_000683"
     CDS             14160..15488
                     /locus_tag="VUW50_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7982627.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            15740..16606
                     /locus_tag="VUW50_000684"
     CDS             15740..16606
                     /locus_tag="VUW50_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7982628.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            16622..17461
                     /locus_tag="VUW50_000685"
     CDS             16622..17461
                     /locus_tag="VUW50_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7982629.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            17734..18186
                     /locus_tag="VUW50_000686"
     CDS             17734..18186
                     /locus_tag="VUW50_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET7982630.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            18283..18627
                     /locus_tag="VUW50_000687"
     CDS             18283..18627
                     /locus_tag="VUW50_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982631.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMSWIINGIVNGLIGRYIKLGIY
                     AAKYGISMVRSILSRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAVAWDA
                     HDKIPNNGRINF"
     gene            18914..19558
                     /locus_tag="VUW50_000688"
     CDS             18914..19558
                     /locus_tag="VUW50_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET7982632.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEIKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSAFLLFGFGLLAFLQVGVMEKLFAKYE"
     gene            19731..20648
                     /locus_tag="VUW50_000689"
     CDS             19731..20648
                     /locus_tag="VUW50_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET7982633.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            20666..21550
                     /locus_tag="VUW50_000690"
     CDS             20666..21550
                     /locus_tag="VUW50_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET7982634.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASMTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(21566..22417)
                     /locus_tag="VUW50_000691"
     CDS             complement(21566..22417)
                     /locus_tag="VUW50_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET7982635.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            22617..24674
                     /gene="pbp2b"
                     /locus_tag="VUW50_000692"
     CDS             22617..24674
                     /gene="pbp2b"
                     /locus_tag="VUW50_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HET7982636.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSELTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            24685..25281
                     /gene="recR"
                     /locus_tag="VUW50_000693"
     CDS             24685..25281
                     /gene="recR"
                     /locus_tag="VUW50_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HET7982637.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            25457..26500
                     /locus_tag="VUW50_000694"
     CDS             25457..26500
                     /locus_tag="VUW50_000694"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET7982638.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQ
                     SGDFIKTQEFSHDPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALELAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            26584..27957
                     /locus_tag="VUW50_000695"
     CDS             26584..27957
                     /locus_tag="VUW50_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET7982639.1"
                     /translation="MKLTIHEIAQVVGVKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIHQAFQGLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSIQLHNQMILSLSPDVLDTVIFYGENIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGVHDQILLKGSNSMNLAKLVE
                     SLENEAK"
     gene            27944..28555
                     /locus_tag="VUW50_000696"
     CDS             27944..28555
                     /locus_tag="VUW50_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7982640.1"
                     /translation="MKPSDFVKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQGSDLDVEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            28646..29350
                     /locus_tag="VUW50_000697"
     CDS             28646..29350
                     /locus_tag="VUW50_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HET7982641.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGRSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            29569..30942
                     /gene="ftsA"
                     /locus_tag="VUW50_000698"
     CDS             29569..30942
                     /gene="ftsA"
                     /locus_tag="VUW50_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET7982642.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVESVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            30959..32218
                     /gene="ftsZ"
                     /locus_tag="VUW50_000699"
     CDS             30959..32218
                     /gene="ftsZ"
                     /locus_tag="VUW50_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET7982643.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETAELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     ISPVERFEAPISQDEDELDTPPFFKNR"
     gene            32223..32894
                     /locus_tag="VUW50_000700"
     CDS             32223..32894
                     /locus_tag="VUW50_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET7982644.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLSLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            32904..33443
                     /locus_tag="VUW50_000701"
     CDS             32904..33443
                     /locus_tag="VUW50_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HET7982645.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTSVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            33443..33706
                     /locus_tag="VUW50_000702"
     CDS             33443..33706
                     /locus_tag="VUW50_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET7982646.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            33703..34488
                     /locus_tag="VUW50_000703"
     CDS             33703..34488
                     /locus_tag="VUW50_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET7982647.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            34497..35285
                     /gene="divIVA"
                     /locus_tag="VUW50_000704"
     CDS             34497..35285
                     /gene="divIVA"
                     /locus_tag="VUW50_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HET7982648.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            35633..38425
                     /locus_tag="VUW50_000705"
     CDS             35633..38425
                     /locus_tag="VUW50_000705"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET7982649.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGETRKFVVAAELLTSLSEK
                     FGWVDVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEYVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPTQDAVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYANFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
     gene            38835..39197
                     /locus_tag="VUW50_000706"
     CDS             38835..39197
                     /locus_tag="VUW50_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982650.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNALKIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGGVSLIPFLGWVGGILA
                     IIGGSLFLATLKKFKSEE"
     gene            39731..40423
                     /locus_tag="VUW50_000707"
     CDS             39731..40423
                     /locus_tag="VUW50_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HET7982651.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            40476..42887
                     /locus_tag="VUW50_000708"
     CDS             40476..42887
                     /locus_tag="VUW50_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET7982652.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMSWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDEKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYSGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            43137..43838
                     /locus_tag="VUW50_000709"
     CDS             43137..43838
                     /locus_tag="VUW50_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982653.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            43840..46548
                     /locus_tag="VUW50_000710"
     CDS             43840..46548
                     /locus_tag="VUW50_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7982654.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQCYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVATVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(46644..47135)
                     /gene="tpx"
                     /locus_tag="VUW50_000711"
     CDS             complement(46644..47135)
                     /gene="tpx"
                     /locus_tag="VUW50_000711"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET7982655.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(47258..48187)
                     /gene="psaA"
                     /locus_tag="VUW50_000712"
     CDS             complement(47258..48187)
                     /gene="psaA"
                     /locus_tag="VUW50_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HET7982656.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(48219..49067)
                     /locus_tag="VUW50_000713"
     CDS             complement(48219..49067)
                     /locus_tag="VUW50_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET7982657.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(49064..49786)
                     /locus_tag="VUW50_000714"
     CDS             complement(49064..49786)
                     /locus_tag="VUW50_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET7982658.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            50077..51969
                     /gene="pepO"
                     /locus_tag="VUW50_000715"
     CDS             50077..51969
                     /gene="pepO"
                     /locus_tag="VUW50_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HET7982659.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(52092..52721)
                     /locus_tag="VUW50_000716"
     CDS             complement(52092..52721)
                     /locus_tag="VUW50_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7982660.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KTICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            53680..55902
                     /locus_tag="VUW50_000717"
     CDS             53680..55902
                     /locus_tag="VUW50_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET7982661.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYPSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            55932..56375
                     /locus_tag="VUW50_000718"
     CDS             55932..56375
                     /locus_tag="VUW50_000718"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HET7982662.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKRGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(56642..56827)
                     /locus_tag="VUW50_000719"
     CDS             complement(56642..56827)
                     /locus_tag="VUW50_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982663.1"
                     /translation="MKKIIFIKTIQLLVIDGIMLAFLTFKRGLTWDWILIYSGWLIFF
                     HPVLLTYLSNQLCDHFS"
     gene            complement(56846..57259)
                     /locus_tag="VUW50_000720"
     CDS             complement(56846..57259)
                     /locus_tag="VUW50_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982664.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            complement(57390..58037)
                     /locus_tag="VUW50_000721"
     CDS             complement(57390..58037)
                     /locus_tag="VUW50_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HET7982665.1"
                     /translation="MVVGIAIASGYLNSHLDKFVDWGSWTALVPFGLISVTNVGISML
                     STRFTGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWEASQE
                     GRPNQMSQKQVKLAAIIISIIAFLFAFVTNYIGYGGKMNLLAYVTTIAFALSLIANAL
                     NALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVR"
BASE COUNT        17939 a        10373 c        12838 g        17032 t
ORIGIN      
        1 gtcagtctta tctacaacct caaaacagtg ttttgagcat catgcggcta gcttcttagt
       61 ttgctctttg attttcattg agtataaaaa cagatgagtt tctgttttct ttttatggac
      121 tataaatgtt cagctgaaac tactttcaag gacattatta tataaaagaa ttttttgaaa
      181 ctaaaatcta ctatattaca ctatattgaa agcgttttaa aaatgaggta taataaattt
      241 actaacgctt ataaaaagtg atagaatcta tttttatgta tatttaaaga tagattgctg
      301 taaaaatagt agtagctatg cgaaataaca gatagagaga agggattgaa gcttagaaaa
      361 ggggaataat atgatattta aggcattcaa gacaaaaaag cagagaaaaa gacaagttga
      421 actacttttg acagtttttt tcgacagttt tctgattgat ttatttcttc acttatttgg
      481 gattgtcccc tttaagctgg ataagattct gattgtgagc ttgattatat ttcccattat
      541 ttctacaagt atttatgctt atgaaaagct atttgaaaaa gtgttcgata aggattgagc
      601 aggaagtatg gtgtaaatag cataggctga tgtccatcat ttgcttataa agagatattt
      661 tagtttaatt gcagcggtgt cctggtagat aaactagatt ggcaggagtc tgattggaga
      721 aaggagaggg gaaaattggc accaatttga gatagtttgt ttagttcatt tttgtcattt
      781 aaatgaactg tagtaaaaga aagttaataa aagacaaact aagtgcattt tctggagtaa
      841 atgtcttatt tcagaaatcg ggatatagat atagagagga tcagtatgaa tcggagtgtt
      901 caagaacgta agtgtcgtta tagcattagg aaactatcgg taggagcggt ttctatgatt
      961 gtaggagcag tggtatttgg aacgtctcct gttttagctc aagaaggggc aagtgagcaa
     1021 cctctggcaa atgaaactca actttcgggg gagagctcaa ccctaactga tacagaaaag
     1081 agccagcctt cttcagagac tgaactttct ggcaataagc aagaacaaga aaggaaagat
     1141 aagcaagaag aaaaaattcc aagagattac tatgcacgag atttggaaaa tgtcgaaaca
     1201 gtgatagaaa aagaagatgt tgaaaccaat gcttcaaatg gtcagagagt tgatttatca
     1261 agtgaactag ataaactaaa gaaacttgaa aacgcaacag ttcacatgga gtttaagcca
     1321 gatgccaagg ccccagcatt ctataatctc ttttctgtgt caagtgctac taaaaaagat
     1381 gagtacttca ctatggcagt ttacaataat actgctactc tagaggggcg tggttcggat
     1441 gggcaacagt tttacggtaa ttacaacgat gcacccttaa aagttaaacc aggtcagtgg
     1501 aattctgtga ctttcacagt tgaaaaaccg acagcagaac tacctaaagg ccgagtgcgc
     1561 ctctacgtaa acggggtatt atctcgaaca agtctgaaat ctggcaattt cattaaagat
     1621 atgccagatg taacgcatgt gcaaatcgga gcaaccaagc gtgccaacaa tacggtttgg
     1681 gggtcaaatc tacagattcg gaatctcact gtgtataatc gtgctttaac accagaagag
     1741 gtacaaaaac gtagtcaact ttttaaacgc tcagatttag aaaaaaaact acctgaagga
     1801 gcggttttaa cagagaaaac ggacatattc gaaagcgggc gtaacggtaa accaaataaa
     1861 gatggaatca agagttatcg tattccagca cttctcaaga cagataaagg aactttgatc
     1921 gcaggtgcag atgaacgccg tctccattcg agtgactggg gtgatatcgg tatggtcatc
     1981 agacgtagtg aagataatgg aaagacatgg ggagataagg tggttatctc caatcttcga
     2041 gataatcctg aagctaaaga tcctgctgcg ccatcgcctc taaatattga tatggttttg
     2101 gttcaagacc cgacaacaaa gagaatcttc tcaatttatg atatgttccc agaaggtcga
     2161 gcagtttttg gaatgccaaa aacacctgaa aaagcttatg aaaagatagg ggataaaact
     2221 tatcaaatct tgtataaaca aggagagtct ggtcattata ctgttcgtga gaatggagaa
     2281 gtgtataatg cacaaaatca aaagacggat tatcgtgttg tagtgaatcc aacagaacct
     2341 ggctatagag ataaaggaaa tctttacaaa ggtcaggaat tgattggaaa tatctatttt
     2401 gcacacagta caaaaaatcc atttagagta gccaatacga gctatctatg gatgtcatat
     2461 agtgacgatg atggtaaaac ttggtctgca ccgagagaca ttactccagg tcttcgcaag
     2521 gattggatga agttcctagg aacaggtcct ggaacaggaa ttgtacttcg gaatgggcct
     2581 cacaagggac ggattttgat accggtttat acgactaata atgtatctca cttaaatggc
     2641 tcgcaatctt ctcgtgtcat ctattcagat gatcatggaa aaacttggca tgctggagaa
     2701 gcggtcaacg ataaccgtca ggtagacggt caaaagatcc actcttctac gatgaacaat
     2761 gaacgtgcgc aaaatacaga atcaacggtg gtacaactaa acaatggaga tgttaaactc
     2821 tttatgcgtg gtttgactgg agatcttcag gttgctacaa gtaaagacgg aggagtgact
     2881 tgggagaagg atatcaaacg ttatccacag gttaaagatg tctatgttca aatgtctgct
     2941 atccatacga tgcacgaagg aaaagaatac atcatcctca gtaatgcagg tggaccgaaa
     3001 cgtgaaaatg ggatggtcca cttggcacgt gtcgaagaaa atggtgagtt gacttggctc
     3061 aaacacaatc caattcaaaa aggagagttt gcctataatt cgctccaaga attaggaaat
     3121 ggggagtatg gtatcttgta tgaacatact gaaaaaggac aaaatgccta taccctatca
     3181 tttagaaaat ttaattggga ctttttgagc aaagatctga tttctcctac cgaagcgaaa
     3241 gtgaagcgaa ctagagagat gggcaaagga gagatgggca aaggagttat tggcttggag
     3301 ttcgactcag aagtattggt caacaaggct ccaacccttc aattggcaaa tggtaaaaca
     3361 gcgactttcc taacccagta tgatagcaag accttgttat ttgcagtaga taaggaagat
     3421 atcggacagg aaattattgg tatagctaaa ggaagcatcg aaagtatgca taatcttcct
     3481 gtaaatctag caggtgccag agttcctggc ggagtaaatg gtagcaaagc agcggtgcat
     3541 gaagttccag aatttacagg gggagttaat ggtacagagc cagctgttca tgaaatcgca
     3601 gagtataagg gatctgattc gcttgtaact cttactacaa aagaagatta tacttacaaa
     3661 gctcctcttg ctcagcaggc acttcctgaa acaggaaaca aggagagtga cctcctagct
     3721 tcactaggac taacagcttt cttccttggt ctgtttacgc tagggaaaaa gagagaacaa
     3781 taagagaaga attctaaaca tttgattttg taaaaataga aggagatagc aggttttcaa
     3841 gcctgctatc tttttttgat gacattcaag ctgatacgaa atcataagag gtatgaaact
     3901 actttcaggg tagtctgttc tataaaatat agtagattga aataagatgt gaacaactct
     3961 accaggaaag tcaaattaat ttatagaatt attttagcag tcaaggtgta ctgttatata
     4021 ttcaatatat tatatgacta ttaaccttgt cttctcctaa aattgacttt cttgttttct
     4081 tatcttgtcc actcgaaaca agtattgtaa gaatttgatt atttttgaaa gtacttttaa
     4141 tatacttgat atagttaaaa aagatttgaa actaaattcc aaattagaaa aagacttgaa
     4201 atactaaaaa aaaaagtata ctctaattga aaacggtaac aaaactaatt tagagaatga
     4261 aatatagagt atttctctct taaaagtttt tggtgaaacg agatgtagaa aggagattta
     4321 gccaaagagt ctattagtgc tagaataata gattagaatt attttagaaa aacgaagtga
     4381 gcagcttata aattcaagtc cccaaataga ttcatactag tatcttttgc aaaaaataaa
     4441 gggcgacttc cttcatgaat atcaatttca tctataagga aggtagctaa ttgaactaac
     4501 ttatttattc tgtttgtcgc tagaaaaatc agacctcctt gtgaagattg aggagatact
     4561 taatgaaaat caaagaagaa actagcaagc tagtagcaga ttgcccaaaa caccgctttg
     4621 aggttgtaga taagactgac gtatataatc caaggtgaag cgactgtggt ttgaagagat
     4681 tttcaaagag tataggctag agagtagtgt ttttatgtcc ttctagtaga aaatgctaga
     4741 cagaagaatg gggaacttgg ataggaaaaa tagattgaga aaggaggtta gaagagatga
     4801 ttattacaaa aattagccgt ttaggaactt atgtgggagt aaatccacat tttgcaacat
     4861 taatagattt tctagaaaaa acaggactag aaaatttaac agaaggttcg attgctatcg
     4921 atggtaatcg attgtttggg aattgcttta cttatctagc agatggtcaa gcaggggctt
     4981 tctttgaaac acaccaaaaa tatttggata ttcatttagt tttggaaaac gaagaagcca
     5041 tggctgttac atcgccggaa aatgtaagcg ttacccaaga atatgatgaa gagaaagata
     5101 ttgaattata cacagggaaa gtggaacagt tggttcattt gagagctggt gaatgcctca
     5161 tcacttttcc agaagattta catcaaccca aggttcgtat aaatgatgaa cctgtgaaaa
     5221 aagttgtctt taaagttgcg atttcttaat gtagaaagag aagaacgatg aaaaaaatga
     5281 gaaagttttt atgtctagct ggaattgcgc tagcggctgt tgccttggta gcttgttcag
     5341 gaaaaaaaga agctacaact agtactgaac caccaacaga attatctggt gagattacaa
     5401 tgtggcactc ctttactcaa ggaccccgtt tagaaagtat tcaaaaatca gcagatgctt
     5461 tcatgcaaaa gcatccaaaa acgaaaatca agattgaaac attttcttgg aatgacttct
     5521 atactaaatg gactacaggt ttagcaaatg gaaatgtgcc agatatcagt acagctcttc
     5581 ctaaccaagt aatggaaatg gtcaactcag acgctttggt tccgctaaat gattctatca
     5641 agcgtattgg acaagataaa tttaacgaaa ctgccttaaa tgaagcaaaa atcggagatg
     5701 attactactc tgttcctctt tattcacatg cacaagtcat gtgggttaga acagatttgt
     5761 taaaagaaca taatattgag gttcctaaaa cttgggatca actctatgaa gcttctaaaa
     5821 aattgaaaga agctggagtt tatggcttgt ctgttccgtt tggaacaaat gacttaatgg
     5881 caacacgttt cttgaacttc tacgtacgta gtggtggagg aagcctctta acaaaagatc
     5941 ttaaagcaga cttgacaagc caacttgctc aagatggtat taaatactgg gttaaattgt
     6001 ataaagaaat ctcacctcaa gattctttga actttaatgt ccttcaacaa gctaccttgt
     6061 tctatcaagg aaaaacagca tttgacttta actctggctt ccatatcgga ggaattaatg
     6121 ccaacagtcc tcaattgatt gattcgattg atgcttatcc tattccaaaa atcaaagagt
     6181 ctgataaaga tcaaggaatt gaaacctcaa acattccaat ggttgtttgg aaaaattcaa
     6241 aacatccaga agttgctaaa gcattcttag aagcacttta taatgaagaa gactacgtta
     6301 aattccttga ttcaactcca gtaggtatgt tgccaactat taaggggatt agcgattctg
     6361 cagcctataa agaaaatgaa actcgtaaga aatttaaaca tgctgaagaa gtaattactg
     6421 aagctgttaa aaaaggtact gctattggtt atgaaaatgg gccaagtgta caagctggta
     6481 tgttgactaa ccaacacatt attgaacaaa tgttccaaga tatcattaca aatggaacag
     6541 atcctatgaa agcagcaaaa gaagcagaaa aacaattaaa cgatttattt gaggctgttc
     6601 agtagatgta aaagactaga aaataggtgg gctagtgagc tgaaaagctc tagcccaatc
     6661 ttgtaaaaga agggagaagg agaatggtta aagaacgtaa tttaacttgc tggatatttg
     6721 ttttgccagc tatgattatc gtaggattac tctttgttta tccgtttttc tcgagtattt
     6781 tttatagctt taccaataag catttgatta tgcctaatta taaatttgtt ggtttggcta
     6841 actataaagc tgtgctatca gatcccaact tctttaatgc gttctttaat tcaattaagt
     6901 ggaccatttt ctcattagtt ggtcaagttt tagtagggtt tgtattggct ttagctcttc
     6961 acagagtacg ccacttcaag aaattatata ggaccttatt gattgttcct tgggcatttc
     7021 ctaccatcgt tattgccttc tcttggcagt ggattctaaa cggggtttat ggctacttac
     7081 ctaatctaat cgtaaaatta ggtttaatgg aacatacacc tgcatttttg acagatagta
     7141 catgggcatt cctatgtttg gtgtttatca acatttggtt tggagcacca atgattatgg
     7201 ttaatgtgct ttcagctttg caaacagtac cagaagaaca atttgaggct gctaagatag
     7261 atggtgcttc aagttggcag gtgttcaagt ttatcgtctt tccacatatt aaagtggttg
     7321 taggacttct agttgttttg agaactgtat ggatctttaa taactttgac attatctacc
     7381 tcattactgg tggtggacca gccaatgcta caacgacgct tccaattttt gcttacaacc
     7441 tgggttgggg aactaaattg ttgggtcgtg cttcagcagt tacagtattg ctctttatct
     7501 tcttggtggc gatttgcttt atctactttg ctatcatcag taagtgggaa aaggagggta
     7561 gaaaataatg aagaagaaat ccggtattta tttagatatt ctctcacatg tacttttagt
     7621 tggtgcgacc atcgttgcag ttttcccatt ggtatggatt atcatatctt ctgtcaaagg
     7681 gaaaggggaa ttaactcagt atccaacacg attttggcct gaacagttta cattagatta
     7741 tttcactcat gttatcaacg atttgcactt cattgataat attcgaaaca gtttaatcat
     7801 tgccttggct acaaccctca ttgcgattat tatttctgct atggcagcct atggtattgt
     7861 tcgattcttt cctaaattgg gagcaatcat gtcgagacta ctcgtcatta cctacatttt
     7921 cccaccaatt ttgttagcaa ttccctattc aattgccatt gctaaagttg ggttaacaaa
     7981 tagtttattt ggcttgatga tggtttatct atcttttagt gttccatatg cagtttggct
     8041 cttagttgga tttttccaaa cagttccaat tggaattgaa gaagcggcta gaattgatgg
     8101 tgcaaataaa tttgttacgt tttataaagt tgtgctaccg attgtagcac caggtattgt
     8161 agcaacagct atttatacat ttatcaatgc ttggaatgaa ttcctgtatg ccttgatttt
     8221 gattaacaat acaggaaaga tgacagtagc agtagccctt cgttcactta atggttcaga
     8281 aatactagac tggggagata tgatggcagc gtctgttatt gtagttcttc catcaattat
     8341 tttcttctct atcatccaaa ataagattgc aagtggatta tcagaaggat ctgtgaagta
     8401 gacgaaagaa ggaaaaaaat gaataaaaga ggtctttatt caaaactagg aatttctgtt
     8461 gtaggcatta gtcttttaat gggagttccc actttgattc atgcgaatga attaaactat
     8521 ggtcaactgt ccatatctcc tatttttcaa ggaggttcat atcaactgaa caataagagt
     8581 atagatatca gctctttgtt attagataaa ttgtctggag agagtcagac agtagtaatg
     8641 aaatttaaag cagataaacc aaactctctt caagctttgt ttggcctatc taatagtaaa
     8701 gcaggcttta aaaataatta cttttcaatt ttcatgagag attctggtga gataggtgta
     8761 gaaataagag acgcccaaaa gggaataaat tatttatttt ctagaccagc ttcattatgg
     8821 ggaaagcata aaggacaggc agttgaaaat acactagtat ttgtatctga ttctaaagat
     8881 aaaacataca caatgtatgt taatggaata gaagtgttct ctgaaacagt tgatacattt
     8941 ttgccaattt caaatataaa tggtatagat aaggcaacac taggagctgt taatcgtgaa
     9001 ggcaaggaac attacctcgc aaaaggaagt attgatgaaa tcagtctatt taacaaagca
     9061 attagtgatc aggaagtttc aaatattccc ttgtcaaatc catttcagtt aattttccaa
     9121 tcaggagatt ctactcaagc taactatttt agaataccga cactatatac attaagtagt
     9181 ggaagagttc tatcaagtat tgatgcacgt tatggtggga ctcatgattc taaaagtaag
     9241 attaatattg ccacttctta tagtgatgat aatgggaaaa cgtggagtga gccaattttt
     9301 gctatgaagt ttaatgacta tgaggagcag ttagtttact ggccacgaga taataaatta
     9361 aagaatagtc aaattagtgg aagtgcttca ttcatagatt catccattgt tgaagataaa
     9421 aaatctggga aaacgatatt actagctgat gttatgcctg cgggtattgg aaataataat
     9481 gcaaataaag ccgactcagg ttttaaagaa ataaatggtc attattattt aaaactaaag
     9541 aagaatggag ataacgattt ccgttataca gttagagaaa atggtgtcgt ttatgatgaa
     9601 acaactaata aacctacaaa ttatactata aatgataagt atgaagtttt ggagggagga
     9661 aagtctttaa cagtcgaaca atattcggtt gattttgata gtggctcttt aagagaaaaa
     9721 cataatggaa aacaggtccc tatgaatgtt ttctacaaag attcgttatt taaagtgact
     9781 cctactaatt atatagcaat gacaactagt cagaatagag gagagagttg ggaacaattt
     9841 aagttgttgc ctccgttctt aggagaaaaa cataatggaa cttacttatg tcccggacaa
     9901 ggtttagcat taaaatcaag taacagattg atttttgcaa catatactag tggagaacta
     9961 acctatctca tttctgatga tagtggtcaa acatggaaga aatcctcagc ttcaattccg
    10021 tttaaaaatg caacagcaga agcacaaatg gttgaactga gagatggtgt gattagaaca
    10081 ttctttagaa ccactacagg taagatagct tatatgacta gtagagattc tggagaaaca
    10141 tggtcgaaag tttcgtatat tgatggaatc caacaaactt catatggcac acaagtatct
    10201 gcaattaaat actctcaatt aattgatgga aaagaagcag tcattttgag tacaccaaat
    10261 tctagaagtg gccgtaaggg aggtcaatta gttgtcggtt tagtcaataa agaagatgat
    10321 agtattgatt ggaaatacca ctatgatatt gatttgcctt cgtatggtta tgcctattct
    10381 gcgattacag aattgccaaa tcatcacata ggtgtactgt ttgaaaaata tgattcgtgg
    10441 tcgagaaatg aattgcattt aagcaatgta gttcagtata tagatttgga aattaatgat
    10501 ttaacaaaat aaaggagaaa aacatggtta aatacggtgt tgttggagca gggtattttg
    10561 gagctgaatt ggctcgctac atgcaaaaga atgatggagc agagattact cttctctatg
    10621 atccagataa tgcagaggcg attgcagaag aattgggagc aaaagtagca agttccttag
    10681 atgagttggt ttctagcgat gaagtagatt gtgttatcgt cgcaactcca aataatcttc
    10741 ataaggaacc ggttattaag gctgcacagc atggtaaaaa tgttttctgt gaaaaaccaa
    10801 ttgcgctttc ttatcaagat tgtcgcgaga tggtagatgc gtgtaaagaa aacaatgtaa
    10861 cctttatggc aggacatatt atgaatttct ttaatggtgt tcatcatgca aaagaactca
    10921 ttaatcaagg agttatcgga gacgttctat attgtcatac agctcgtaat ggttgggaag
    10981 aacaacaacc gtcagtatca tggaaaaaaa ttcgtgaaaa atcaggtggt cacttgtatc
    11041 accacatcca tgaattggat tgcgttcaat tccttatggg gggcatgcct gaaactgtaa
    11101 ccatgacagg tggaaatgtg gcccatgaag gtgaacattt cggtgatgaa gatgatatga
    11161 tttttgtcaa tatggaattt tctaataagc gttttgcttt gttagaatgg ggttcagctt
    11221 atcgttgggg tgaacattat gtcttaatcc aaggaagcaa aggtgccatc cgcttagact
    11281 tattcaactg taaaggaact cttaagctag atgggcaaga aagctatttc ttgattcacg
    11341 aatcgcaaga agaagatgat gatcgaactc gtatctatca tagtacagag atggatggag
    11401 caattgctta tggtaaacca ggtaaacgta ctccattatg gctatcatct gtcattgata
    11461 aagaaatgcg ctatctgcat gagattatgg aaggagctcc agtatcagaa gaatttgcaa
    11521 aacttttgac aggtgaagct gccctagaag caattgctac tgcagatgct tgtacccagt
    11581 ctatgtttga agatcgcaaa gtaaaattgt cagaaattgt aaaataaatt ttggtattct
    11641 cctatttata ggtcgacttg ctcctctgaa agtactttta gaggagctgt ttgactttgc
    11701 tagtttttga aactgaaatc tattatacta caaactattg aaagcgtttt aattttaagg
    11761 tataataatc tcatagaaat aaagaaaagg aggaaagagg atgccacaga ttagcaaaga
    11821 agccttgatt gagcaaatca aagatggaat catcgtttct tgtcaggctc ttcctcatga
    11881 accgctttat acagaagcgg gaggggtgat tcccttgctg gtcaaagcgg ctgagcaagg
    11941 tggagcagtc ggtatccgag caaacagtgt tcgcgatatc aaggaaatta aggaagtcac
    12001 taaacttcca atcattggga ttatcaaacg tgattatcca cctcaggaac ccttcatcac
    12061 ggctactatg aaagaagttg atgaattggc agaactggac atcgaggtga ttgctctgga
    12121 ttgtaccaag cgtgaacgct acgatggttt ggaaattcaa gagttcattc gtcaggttaa
    12181 ggagaaatat cctaatcagc ttttgatggc tgatactagt atcttcgaag aagggctagc
    12241 agctgtagaa gcaggaattg actttgtcgg aacaacctta tcaggctaca catcctacag
    12301 tccaaaagta gacggtccag attttgaatt gattaagaaa ctctgtgatg ccggtgtaga
    12361 tgtcattgca gaaggaaaaa ttcatacacc agaacaagcc aaacaaatcc ttgaatatgg
    12421 agtgcgaggc atcgttgttg gtggcgccat tactagacca aaagagatta cagaacgctt
    12481 cgttgctagt cttaaataag atgtgagggg gagttttatg tttaaagttt tacaaaaagt
    12541 tggaaaagct tttatgttac ctatagctat acttcctgca gcaggtctac ttttggggat
    12601 tggtggtgca ctttcaaacc caaccacgat agcaacttat ccaatactag acaatagtat
    12661 ttttcaatca atattccaag taatgagctc tgcaggagag gttgtattca gtaatttgtc
    12721 actacttctc tgtgtgggat tatgtattgg cttagcgaaa cgagataaag gaaccgctgc
    12781 gttagcagga gtaactggtt acttagttat gactgcaacg atcaaagctt tagtaaaact
    12841 ttttatggca gaaggatctg caattgatac tggagttatt ggagcattag ttgtcggaat
    12901 agttgccgta tatttgcaca accgatataa caatattcaa ttaccttccg ctttaggatt
    12961 ctttggaggt tcacgcttcg ttcctattgt tacatcgttc tcttctatct tgattggctt
    13021 tgtcttcttt gttatttggc cacctttcca acaacttctt gtttctacag gtggatatat
    13081 ttctcaggcg ggtccaattg gaacttttct atatggattt ttaatgagac tttctggagc
    13141 agtaggctta catcatataa tttaccctat gttttggtat actgaacttg gtggtgttga
    13201 aactgttgca ggacaaacag tggttggagc tcaaaaaata ttttttgctc aattagccga
    13261 tttggcccat tctggattat ttacagaagg aacaaggttt tttgcaggtc gtttctcaac
    13321 aatgatgttc ggtttaccgg ctgcctgttt agcgatgtac catagtgttc ctaaaaatcg
    13381 tcgtaaaaaa tacgcgggtt tgttttttgg agttgcttta acatctttta ttaccggtat
    13441 tacagaacca attgaattta tgtttctatt cgttagtccg gttctatatg ttgttcacgc
    13501 attccttgat ggtgttagct tctttattgc agacgtctta aatatttcaa taggaaacac
    13561 attttcagga ggtgtaatcg atttcacttt atttggaatt ttgcagggga acgctaagac
    13621 gaattgggtt cttcagattc catttggact tatttggagt gttttgtatt atattatttt
    13681 tagatggttc attactcaat tcaacgttct aacgccaggg cgaggagaag aagtagattc
    13741 taaagaaatt tctgaatccg cagattcaac ttcaaatact gcagattatt taaaacagga
    13801 tagcctacaa attatcagag ccttgggtgg atcaaataat atagaagatg tagatgcttg
    13861 tgtgacacgt ttacgtgtag ctgtaaaaga agttaatcaa gttgataaag cacttttaaa
    13921 acaaattggt gcagttgatg tcttagaagt gaagggtggc attcaagcaa tctatggagc
    13981 aaaagcaatc ttatataaaa atagtattaa tgaaatttta ggtgtagatg attaagtact
    14041 tactgactta ataaaaaaca gaggagagtg atggatgagt aggatgaaat gaaatcgcat
    14101 acaagaaata aagaactcat tatccaagtt ggatacgctt attacatagg agaatacaaa
    14161 tgaaatttag aaaattagct tgtacagtac ttgcgggtgc tgcggttctt ggtcttgctg
    14221 cttgtggcaa ttctggcgga agtaaagatg ctgccaaatc aggtggtgac ggtgccaaaa
    14281 cagaaatcac ttggtgggca ttcccagtat ttacccaaga aaaaactggt gacggtgttg
    14341 gaacttatga aaaatcaatc atcgaagcgt ttgaaaaagc aaacccagat ataaaagtga
    14401 aattggaaac catcgacttc aagtcaggtc ctgaaaaaat cacaacagcc atcgaagcag
    14461 gaacagctcc agacgtactc tttgatgcac caggacgtat catccaatac ggtaaaaacg
    14521 gtaaattggc tgagttgaac gacctcttca cagatgaatt tgttaaagat gtcaacaatg
    14581 aaaacatcgt acaagcaagt aaagctggag ataaggctta tatgtatccg attagttctg
    14641 ccccattcta catggcaatg aacaagaaaa tgttagaaga tgctggagta gcaaaccttg
    14701 taaaagaagg ttggacaact gatgattttg aaaaagtatt gaaagcactt aaagacaagg
    14761 gttacacacc aggttcattg ttcagttctg gtcaaggggg agaccaagga acacgtgcct
    14821 ttatctctaa cctttatagc ggttctgtaa cagatgaaaa agttagcaaa tatacaactg
    14881 atgatcctaa attcgtcaaa ggtcttgaaa aagcaactag ctggattaaa gacaatttga
    14941 tcaataatgg ttcacaattt gacggtgggg cagatatcca aaactttgcc aacggtcaaa
    15001 catcttacac aatcctttgg gcaccagctc aaaatggtat ccaagctaaa cttttagaag
    15061 caagtaaggt agaagtggta gaagtaccat tcccatcaga cgaaggtaag ccagctcttg
    15121 agtaccttgt aaacgggttt gcagtattca acaataaaga cgacaagaaa gtcgctgcat
    15181 ctaagaaatt catccagttt atcgcagatg acaaggagtg gggacctaaa gacgtagttc
    15241 gtacaggtgc tttcccagtc cgtacttcat ttggaaaact ttatgaagac aaacgcatgg
    15301 aaacaatcag cggctggact caatactact caccatacta caacactatt gatggatttg
    15361 ctgaaatgag aacactttgg ttcccaatgt tgcaatctgt atcaaatggt gacgaaaaac
    15421 cagcagatgc tttgaaagcc ttcactgaaa aagcgaacga aacaatcaaa aaagctatga
    15481 aacaatagtc cttagttatt ctataaaaag tagtttttta aagaacctaa gagtgtatac
    15541 ccccttttcc ctctacacag atagtgtaag aaaagggggc ttttgtttaa aatgtaagaa
    15601 actgtcacga aattaaaatg aagttcttac ataagcgaat cataaaaaat ttcattttga
    15661 ttttaaaaca gttcaagaaa gtcaaaaaat tattctattt gaaagagagg tgccgactgt
    15721 gaaagtcaat aaaatccgta tgcgggaaac agtgatttcc tacgctttcc tagcaccagt
    15781 attattcttc tttgtcatct ttgtgttggc tccgatggtg atgggcttca ttacaagttt
    15841 ctttaactac tcaatgacta aatttgagtt tgtaggcttg gataactata tccgtatgtt
    15901 taaagatcct gtctttacaa aatctctgat taacacagtt attttggtta ttggatctgt
    15961 accagttgtt gttctattct cactctttgt agcatctcag acctatcatc aaaatgtcat
    16021 tgccagatcc ttctaccgtt tcgtcttctt ccttcctgtt gtaacgggta gtgttgccgt
    16081 gacagttgtt tggaaatgga tttatgaccc gctatcaggg attctaaact ttgtccttaa
    16141 gtcaagccac atcatcagcc aaaacatttc ttggttggga gataaaaact gggcattgat
    16201 ggcgattatg attattctct tgaccacttc agttggtcag cccatcatcc tttatatcgc
    16261 tgccatgggg aatattgaca attcactggt tgaagcggcg cgtgttgatg gtgcaactga
    16321 gtttcaagtt ttttggaaga ttaaatggcc aagccttctt ccaacaactc tttatattgc
    16381 aatcatcaca acaattaact cattccagtg tttcgccttg attcagcttt tgacatctgg
    16441 tggtccaaac tactcaacaa gtaccttgat gtactacctt tacgaaaaag ccttccaatt
    16501 gacagaatac ggctatgcca acacaattgg tgtcttcttg gcagtcatga ttgctatcgt
    16561 aagctttgtt caatttaaag tacttggaaa cgacgtagaa tactaaagaa aggagacagc
    16621 tatgcaatct acagaaaaaa aaccattaac agcctttact gttatttcaa caatcatttt
    16681 gctcttgttg actgtgctgt tcatctttcc attctactgg attttgacag gggcattcaa
    16741 atcacaacct gatacaattg ttattcctcc tcagtggttc cctaaaatgc caaccatgga
    16801 aaacttccaa caactcatgg tgcagaaccc tgccttgcaa tggatgtgga actcagtatt
    16861 tatctcattg gtaaccatgt tcttagtttg tgcaacctca tctctagcag gttatgtatt
    16921 ggctaaaaaa cgtttctatg gtcaacgcat tctatttgct atctttatcg ctgctatggc
    16981 gcttccaaaa caagttgtcc ttgtaccatt ggtacgtatc gtcaacttca tgggaatcca
    17041 cgatactctc tgggcagtta tcttgccttt gattggatgg ccattcggtg tcttcctcat
    17101 gaaacaattt agtgaaaata ttcctacaga gttgcttgaa tcagctaaaa tcgacggttg
    17161 tggtgagatt cgtaccttct ggagtgtagc cttcccgatt gtgaaaccag ggtttgcagc
    17221 ccttgcaatc tttaccttca tcaatacttg gaatgactac ttcatgcaat tggtaatgtt
    17281 gacttcacgt aacaatttga ccatctcact tggggttgcg accatgcagg ctgaaatggc
    17341 aaccaactat ggtttgatta tggcaggagc tgcccttgct gctgttccaa tcgtcacagt
    17401 cttcctagtc ttccaaaaat ccttcacaca gggtattact atgggagcgg tcaaaggata
    17461 atactctgcg aaaatcgaat gcaaactacg tcagcttcac cttgccatac ttaagtattg
    17521 cctgcggtta gcttcctagt ttgttcttca attttcattg agtataggaa aatcaatcta
    17581 tcaagataca gaagtatatt ttatagattt agagaatata gaggttataa gtgtctacaa
    17641 aatggagggt atgcagttac tttatgaagt tttgtcagac acttatcaac ttaagaatgg
    17701 ttttagttaa ctatcagaaa cgaaggaaag agtatgattt ttgacgattt gaaaaacatc
    17761 accttttaca aagggattca tcccaattta gacaaggcta tcgactacct ctaccaacat
    17821 cgtaaagatt cattcgaatt aggaaagtat gagattgatg gagataaagt ctttctagtt
    17881 gttcaggaaa atgtcctcaa tcaagttgag aataatcaat ttgaacacca taagaactat
    17941 gcagatttgc atttgctgat agaagggcat gaatattcga gctacggttc acgtatcaaa
    18001 gacgaggcag tagcattcga cgaagcgagt gacattggct ttgttcattg tcatgaacac
    18061 tacccactct tattgggtta tcacaatttt gcgattttct tcccaggtga gccacatcag
    18121 ccaaatggtt atgcaggcat ggaagaaaag gttcgaaaat atctctttaa aattttgatt
    18181 gattaaaaat aggatgaatt gtttttttgt aaagctttga caatactcta ccatgaaatt
    18241 gatctttgtg aggtagagaa atgagaataa aatatttaaa aattggtatc ttctaagtat
    18301 gctgcaagag ccagtttctt agatggacag gggattacag ttgatgagat gtcctggata
    18361 attaatggca ttgtgaatgg attgattggt agatacataa aattaggtat ttatgcagcc
    18421 aagtatggta ttagtatggt gcgatcgatc ttaagtaggg cagctgcaac tgcggcagca
    18481 agagtaggat tattgaccaa gatttttgga tggattttac gaatagctgt gctcgcagct
    18541 gatgtatatg gtaattttgc caacaatatt gctgtagctt gggatgcaca tgataaaatc
    18601 cctaacaatg gtcgtataaa cttttaaaat gcgagaatga aagcactttg tattttttta
    18661 ttgaatatgt tagcttggac agtgcttgca atgacagtcc gtggagggct agatggattt
    18721 aatgagcata cttggagtac tattttaatt gcttcgctgt ccgggatata tatagattat
    18781 atgtccatag ataaaagtag aaaaaagtcc aaaagaaaag agaaaaaata gatttgttca
    18841 tggtagggac ttatgaaagc tttactgaca aaaaagaaaa cagtttacaa agaaaaatga
    18901 tggaggagca aacatggcac aaaaaggagt aagccttatc aaggcagcat ttgatacaga
    18961 caactttctc atgcgtttta gtgagaaagt cttggacatc gtgacagcca atcttctttt
    19021 tgtcgtctct tgtttgccca tcgtgacgat tggagtggct aaaatcagcc tctacgagac
    19081 catgtttgag attaagaaga gcagacgggt gcctgttttt aaaatctatc taagatcttt
    19141 caagcaaaat ctgaaactag gtcttcagct gggtttaatg gagttaggaa ttgtgtttct
    19201 taccctttca gatctctatc ttttctgggg tcaaacagct ctgcccttcc aattgctgaa
    19261 agccatttgt ttaggtattc tgatttttct tactatcgtg atgctggcta gttaccctat
    19321 cgcggcacgt tatgacctat cttggaaaga aattcttcaa aaaggattga tgttggctag
    19381 ttttaacttt ccttggttct tcctcatgtt agccattctt gtcctcattg tgatggttct
    19441 ttatctgtct gccttcagtc tactcttagg tggctcagcc ttcctacttt ttgggtttgg
    19501 acttcttgct tttttacaag tgggagtgat ggaaaagcta tttgctaagt atgagtaagg
    19561 ggtgcaatta tttctgaaac tactttcaaa ggctccaaac gctattctat aagcgagaaa
    19621 ctaaaatcta agtgtctata agatattgaa agccttttcg gtaaagtgta tactaaagaa
    19681 gtaaagaaaa atctgcttta gcagaaaata aaaataatag gagaaaatct atgtcagatt
    19741 tgaaaaaata cgaaggtgtc attccagcct tctacgcatg ttatgatgag caaggagaag
    19801 taagtccaga gcgtacgcgt gccttggttc aatacttcat tgataaaggt gttcaaggtc
    19861 tttatgtcaa tggttcttct ggtgaatgta tctaccaaag cgttgaagat cgcaagttga
    19921 ttttggaaga agtcatggcg gtagccaaag gtaaattgac cattattgcc catgttgctt
    19981 gcaataatac taaagatagt atggaacttg ctcgccatgc tgaaagcttg ggagtagatg
    20041 ctattgcaac gattccacca atttacttcc gcttgccaga atactcagtt gccaaatact
    20101 ggaacgatat tagttctgca gctccaaaca cagactacgt gatttacaac attcctcaat
    20161 tggcaggggt tgctttgact ccaagccttt acacagaaat gttgaaaaat cctcgtgtta
    20221 tcggtgttaa gaactcttct atgccagttc aagatatcca aacctttgtc agccttggtg
    20281 gggaagacca tatcgtcttt aacggtccag atgaacagtt cctaggtggc cgcctcatgg
    20341 gggctaaggc tggtatcggt ggtacttatg gtgctatgcc agaactcttc ttgaaactca
    20401 atcagttgat tgcggataag gacctagaaa cagcgcgtga attgcagtat gctatcaacg
    20461 caatcattgg taaactcact tctgctcatg gaaatatgta cggtgtcatc aaagaagtct
    20521 tgaaaatcaa tgaaggcttg aatattggat ctgttcgttc accattgaca ccagtgactg
    20581 aagaagatcg tccagttgta gaagcggctg ctgccttgat tcgtgaaacc aaggagcgct
    20641 tcctctaatc taaaaggagg tatttatgac atattacgtt gcaattgata tcggtggaac
    20701 caacatcaag tatggtttgg ttgatcaaga ggggcaactt cttgaatcgc atgaaatgcc
    20761 aactgaggcg cataagggtg gacctcatat cttacaaaag accaaagata tcgtagctag
    20821 ttatttagaa aaaggcccag tagcaggtgt tgccatatct tctgctggga tggtggatcc
    20881 ggataagggt gagattttct atgctgggcc gcaaatccct aactacgcag gcacccagtt
    20941 caaaaaggaa atcgaagaaa gctttactat tccttgtgag attgaaaatg atgtcaactg
    21001 tgcaggtctt gctgaggcag tatctggttc aggcaaggga gcaagtatga cactttgctt
    21061 gaccattgga accggtatcg gtggttgctt gattatggat aggaaagtct tccatggttt
    21121 tagtaattca gcctgtgaag tcgggtatat gcatatgcaa gatggagctt ttcaggatct
    21181 cgcttctaca acagctttag tgaaatatgt agctgaagcc catggagaag atgttgatca
    21241 gtggaatggc cgacgtatct ttaaggaagc tactgaagga aacaaaatct gcatggaagg
    21301 cattgaccgt atggttgact atctaggaaa aggtctggca aatatttgct acgttgccaa
    21361 tccagaagtg gttattcttg gtggtggtat catggggcaa gaggctatcc tcaaacctaa
    21421 gatccgcaca gccttgaaag aggctttggt accaagttta gcagaaaaaa cacgattaga
    21481 atttgcccat caccaaaata cagcagggat gttgggtgca tattatcatt ttaagacaaa
    21541 acaatcctag tttggctcag ccaaactagg attttcttac acgtttttgt ctacgatagc
    21601 cgttgagttt tttattttcc cagtagctat taaagatttt ttccttgctt tcgcgattga
    21661 tttccaaaaa gtaggcataa atcaaatcga taaagaagag cataggaagt tgagcggata
    21721 ttcgttggat ataggagggt tggctgtggg tggctacaag aacagtctct gtataggtct
    21781 ggctatcttt attgggaaca cttgtaaaga gtacagtctt tgcccccatc tccttggcat
    21841 ctaatagact atctaaaata gaaggagttg agcctgaaag tgagaagccc agtactagac
    21901 aattttcatc catgatgctg gttgtccagg caaagccgtc ttggtctgtc aaagcttcgc
    21961 agaccacacc tagtcgcata aaacgtaatt tcatttcacg ggcgacgagg ccagaactcc
    22021 ctgttccaaa gaagtagata cgctcagcat cttcgattag ctgggcaatt cgctctagtt
    22081 ggatttcgtc aatcaagtct tgtgtttgtt ccctcatatt gctataactt ctgaggactc
    22141 gtttggtcag tggactgtgc ttggagactt ggttggcttg attttctgcc tgatgttggt
    22201 attggaaaat aaattctcgg tagccagtaa agccacactt tttagcaaag cgggtcaaag
    22261 cagcttgaga aatatgtaat ttttgggtga cttgttgaga agataaatca tctgtaatcg
    22321 tttcagcttg caaaaaatag cgagcgattt cttgctctag gtctgtcatt tcttcaaaat
    22381 gtgaatcaat gatagttgcg atatctggtt tgtccatagg gaaagctcct ttacatgagt
    22441 catactggaa gactagatca gggaatagtc acacttcatt ataacacata atataaggat
    22501 agataaataa aaacgcattt ctgttttaaa aacgaaaaaa tcgaaaaagc ttctttcttt
    22561 tccataattt tctactcaaa ttgtggtaca attaagagta agattttaag ttagaaatga
    22621 gactgatttg tatgagaaaa tttaacagcc attcgattcc gattcggctt aatttattgt
    22681 tttcaatcgt cattttactc tttatgacca ttattggtcg tttgttgtat atgcaggttt
    22741 tgaacaagga tttttacgaa aaaaagctag cctcagctag tcagaccaag attacaagca
    22801 gttcagcccg tggggaaatt tatgatgcta gtggaaaacc tttggtagaa aatacgttaa
    22861 agcaggttgt ttcctttacg cgtagcaata aaatgacggc tacagactta aaagaaacag
    22921 ctaaaaagtt actgacttat gtgagcatca gttctccaaa tttgacagaa cgccagctgg
    22981 cggattacta tttggctgat cctgaaatct ataaaaaaac agtggaagct ctcccaagcg
    23041 agaaacgctt ggattcagat ggcaatcgtc tatccgaatc agaactgtat aacaatgcgg
    23101 tcgatagtgt ccaaacaagt caactaaact atacagagga tgaaaagaaa gaaatctatc
    23161 tttttagtca gttaaatgct gttggaaact ttgcgacagg aaccattgct acagatcctc
    23221 taaatgattc tcaggtggct gttattgcct ctatttcaaa ggagatgcct ggcattagta
    23281 tttctacttc ttgggataga aaggttttgg aaacttccct ttcttctata gttgggagtg
    23341 tatccagtga aaaagctggt ctcccagcgg aagaagcaga agcctatctt aaaaaaggct
    23401 attctctaaa tgatcgtgta ggaacctcct atttggaaaa gcaatatgaa gagaccttac
    23461 aaggaaaacg ctcggtaaaa gaaatccatc tggataaata tggcaatatg gaaagcgtgg
    23521 atacaattga ggaaggtagt aagggaaaca atatcaaact gaccattgat ttggccttcc
    23581 aagatagcgt ggatgcttta ctgaaaagtt atttcaattc cgagctagaa aatggtggag
    23641 ccaagtattc tgaaggtgtc tatgcagtcg cccttaaccc aaaaacaggt gcggttttgt
    23701 ctatgtcagg gattaaacat gacttgaaaa cgggagagtt gacgcctgat tccttgggaa
    23761 cggtaaccaa tgtctttgtt ccaggttcgg ttgtcaaggc ggcgaccatc agctcaggtt
    23821 gggaaaatgg agtcttgtca ggaaaccaga ccttgacaga ccagtccatt gtcttccaag
    23881 gttcagctcc catcaattct tggtatactc aggcttacgg ttcattccct atcacagcgg
    23941 tccaagctct ggagtattca tcaaatacct atatggtcca aacagcctta ggtcttatgg
    24001 ggcaaaccta tcaacccaat atgtttgtcg gcaccagcaa tctagagtct gctatggaga
    24061 aactgcgttc aacctttggc gaatatggct tgggtactgc gacaggaatt gacctaccag
    24121 atgaatctac tggatttgtt cccaaagagt atagctttgc taattacatt actaatgcct
    24181 ttgggcagtt tgataactat acgccgatgc agttggctca gtatgtagca actattgcaa
    24241 ataatggtgt tcgtgtggct cctcgtattg ttgaaggcat ttatggtaat aatgataagg
    24301 gaggactggg tgacttgatt cagcaactgc aaccgacaga gatgaataag gtcaatatat
    24361 ccgactccga tatgagcatc ttgcaccaag gtttttatca ggttgcccat ggtactagtg
    24421 aattgacaac tggacgtgcc ttttcaaatg gcgccttggt atccattagc ggaaaaacag
    24481 gtacagccga aagctatgtg gcagatggtc agcaagcaac caataccaat gcggtggcct
    24541 atgccccatc tgataatccc caaatcgctg ttgcagtggt ctttcctcat aataccaatc
    24601 taacaaatgg tgtaggacct tccattgcgc gtgacattat caatctgtat caaaaatacc
    24661 atccaatgaa ctagaaagga aattatgctt tatccaacac ctattgccaa gttgattgac
    24721 agttattcta agttaccagg tatcgggatt aagacggcta cgcgtctggc cttttatacg
    24781 attgggatgt ctgctgatga tgtcaatgaa tttgcaaaaa atctcctttc tgctaagaga
    24841 gaattgacat attgttctat ttgtggacgt ttgacagacg acgatccttg ttctatctgt
    24901 actgatccga ctcgtgacca gacaacaatt ttagttcttg aggatagtag agatgtggca
    24961 gccatggaaa atatccaaga ataccatgga ctctatcatg tccttcatgg cctcatttct
    25021 cctatgaatg gtatcagtcc ggacgatatc aatctcaaga gccttatgac tcgtcttatg
    25081 gatagtgagg tttcagaagt gattgtggcg actaatgcta cagcggatgg tgaagcgact
    25141 tccatgtatc tttcacgttt gctcaagccg gctggtatca aggttacgcg tctagcacga
    25201 ggtctcgctg tgggagcgga cattgagtat gcggacgaag tgacactctt acgagcgatt
    25261 gaaaatcgga cagagttgta agtgtaggca aatttacgaa ctccattcat ttataaaaaa
    25321 tcaaagaggc tgaaaatcgt tcctatcggc ctctttttgt atagtgtgat gagtaggatc
    25381 aggttcaagt tttaaaaaac caagcaaata tgatatacta aagagcgagt attctagtag
    25441 aattaggaca aataatatga aacaaacgat tattctttta tatggtggac ggagtgcgga
    25501 acgcgaagtc tctgtccttt cagctgagag tgtcatgcgt gcggtcgatt acgaccgttt
    25561 cacagtcaag actttcttta tcagtcagtc aggtgacttt atcaaaacac aggaatttag
    25621 tcatgatccg gggcaagaag accgtctcat gaccaatgaa accattgatt gggataagaa
    25681 agttgcacca agtgctatct acgaagaagg tgcagtggtc tttccagtcc ttcacgggcc
    25741 aatgggagaa gatggctctg ttcaaggatt cttggaagtt ttgaaaatgc cttacgttgg
    25801 ttgcaacatt ttgtcatcaa gtcttgccat ggataaaatc acgactaagc gtgttctgga
    25861 atctgctggt attgcccaag ttccttatgt ggctatcgtt gaaggcgatg atgtgactgc
    25921 taaaatcgct gaagtggaag aaaaattggc ttatccagtc ttcactaagc cgtcaaacat
    25981 ggggtctagt gtcggtattt ctaagtctga aaaccaagaa gaactccgtc aagccttaga
    26041 acttgccttc cgatatgata gccgtgtctt ggttgagcaa ggagtgaatg cccgtgaaat
    26101 tgaggttggc ctcttgggta actacgatgt caagagcacg ctaccaggag aagttgtcaa
    26161 ggatgttgcc ttttatgact acgatgccaa gtatattgat aacaagatta ctatggatat
    26221 tcctgccaaa atcagtgatg atgtggtggc tgtcatgcgt caaaatgcag aaacagcctt
    26281 ccgtgccatt ggtggccttg gtctatctcg ttgcgatttc ttctatacag ataagggaga
    26341 gatttttctc aacgagctca ataccatgcc aggtttcacc cagtggtcta tgtacccact
    26401 actttgggac aatatgggga tcagctaccc agaactaatc gagcgtttgg ttgaccttgc
    26461 caaggaaagt tttgacaagc gcgaagcgca tttgatataa aatgaaagag agggtagaag
    26521 ccagaaccat cactgcaagg tgactagagt tctcggactt cagccctttt taaaggagta
    26581 gaaatgaaat taacaatcca tgaaattgcc caagttgttg gagtcaaaaa tgatatcagt
    26641 atctttgagg acacccagtt agaaaaagct gagtttgata gtcgtttgat tggaactgga
    26701 gatttatttg tgccacttaa aggtgcgcgt gatggccatg actttattga aacagccttt
    26761 gaaaatggtg cagcagtaac cttgtctgag aaagaggtct caaatcatcc ttacatttta
    26821 gtagatgatg ttttgacagc ctttcaatcc ctagcatcct actatcttga aaaaacgact
    26881 gttgatgtct ttgctgttac aggttcaaat ggcaagacaa cgactaagga tatgttggcg
    26941 catttactgt caacaagata caagacctac aaaacacaag gcaattacaa taatgagatt
    27001 ggccttcctt acacagttct ccacatgcct gaaggaacag aaaagttggt cttggagatg
    27061 gggcaggacc acttgggaga tattcatctc ttgtctgaat tggctcgtcc aaaaacagcc
    27121 atcgtgacct tggttggaga agctcatttg gcctttttca aagatcgttc tgagattgct
    27181 aaaggaaaaa tgcaaattgc agacggaatg gcttcaggtt ccttgctttt agcgccggct
    27241 gaccctatcg tagaggacta tttgccaact gataaaaagg tggttcgttt tggtcaagga
    27301 gcagagctgg aaattactga cttggttgag cgcaaagata gtctgacctt caaggccaat
    27361 ttcttagagc aagtccttga tttgccagta actggcaagt acaatgcgac aaatgctatg
    27421 attgcatcct atgttgcctt gcaagaagga gtttcagagg agcaaattca tcaggccttc
    27481 caaggtcttg aattgacgcg taatcgtact gagtggaaga aagcagccaa tggagcagat
    27541 atcctgtcag acgtatataa tgccaatcca actgctatga agctgatttt agagactttc
    27601 tctgccattc cagccaatga aggtggcaag aaaattgcag tgttggcgga tatgaaggag
    27661 cttggtgacc agtctattca actccataac cagatgattt tgagcctttc tccagatgtg
    27721 cttgataccg ttattttcta tggagaaaat attgctgaat tagcccaatt ggctagtcaa
    27781 atgttcccaa taggccacgt ttactacttc aagaaaacag aagaccaaga ccaatttgaa
    27841 gacctagtca agcaggtcaa agaaagcctt ggagtccacg accaaatcct gctcaaaggt
    27901 tctaactcta tgaatctagc caaattggta gaaagtttag aaaatgaagc caagtgattt
    27961 tgttaagtat ctgcaaagaa tgattgccct tacagatact ggcttaacct ttacaaagga
    28021 tccttttgac cgtgagcgct acgaggactt gcgaagtctg ttatctgaaa tgttgaatca
    28081 gggatcagac ctggatgtag aagaagtagc agaagtcttg aaaccaactt cagcttatgc
    28141 gactccctta atggacgtcc gtgcttggat tgttgaggat gagaagattt gtctggttag
    28201 gggacaagga gaggatagtt gggctttgcc aggaggcttt ggtgaagtcg gctattctcc
    28261 aactgaaaat attctcaagg aaattgaaga agaaaccggt tttaaagcca aagttgaaag
    28321 actgctagct gtttttgata ccaatcgttt ccaactacag agcaaacaat atgcaaagtt
    28381 tgtttttgaa tgtaagcttc tcgatggaca attccaagaa aatcaagaaa tcgctgacct
    28441 tcaatttttt gcaattgacc aactgccgaa tttatctgaa aaacgcatta ccaaggagca
    28501 aatcgagatt ctttggcagg tttatcaagg tcagagggag caatatcttg actaagaaga
    28561 tgattatcgt atttctaaat tcatttttct taagtagcat ggtataataa aatgcaggaa
    28621 aattttgaat catgaggaag actagatgaa tttatgggat attttcttta cgactcaggc
    28681 aaccgagccg cccaaatttg accttttttg gtatgttagc ctatttacgc tcttagcctt
    28741 aaccttttat acagcccatc gctatcgtga aaagaaggtt taccaacgat ttttccaaat
    28801 cttgcagact gttcagttaa tccttcttta tggttggtac tgggttaatc atatgccact
    28861 gtcagaaagc ctaccctttt accattgccg tatggctatg tttgtggtac tcttgcttcc
    28921 tggtcggtcc aaatataaac aatactttgc attattggga acatttggga cattagcagc
    28981 ctttgtttat ccagtgccag atgcttaccc ttttccacat attaccattt tatcctttat
    29041 ctttggtcat ttagcactct tggggaactc tctagtttat ctattgagac agtataatgc
    29101 gcgattgctg gatgtgaagg gaatttttct catgaccttt gccctaaatg ccttgatttt
    29161 tgtggtcaat ttggtgacag gtggcgatta cggatttttg acaaaaccgc cattggttgg
    29221 ggatcacggt ctagtagcta attatttact tgtttcaatt gtgctggtag ctactatcag
    29281 tttgactaag aaaatcttag aattcttttt agctcaagaa gcagaaaaaa tgattgcaaa
    29341 ggaagcttaa cacagagctt tcttttttgc tcttagagag tttttacaag cagcttataa
    29401 aataagaatt tctgaataga caaactcaaa aaatggctgg gaaatttagg aaaaaagcaa
    29461 gcacgattaa attttttgtg ttataatatt ttgtgaatag ctatgcctat gtttagctat
    29521 ggaataatac gaagtgcgaa acttggaaga tagagaggaa gcgatgtaat ggctagagaa
    29581 ggctttttta caggtctaga tattggaaca agctctgtca aggtgcttgt ggccgagcag
    29641 agaaatggtg aattaaatgt aattggcgtg agtaatgcca aaagtaaagg tgtaaaggat
    29701 ggaattattg ttgatattga tgcagcagca actgctatca agtcagccat ttcccaagcg
    29761 gaagaaaagg caggcatttc gattaaatca gtaaatgtcg gcttgcctgg taatcttttg
    29821 caggtagaac caactcaggg gatgattcca gtaacatctg atactaagga aattacggat
    29881 caagatgttg aaaatgttgt caaatcagct ttgacaaaga gtatgacacc tgaccgtgaa
    29941 gtcattacct ttattcctga agaatttatt gtggatggtt tccaagggat tcgtgaccca
    30001 cgtggcatga tgggggttcg ccttgaaatg cgtggtttgc tttatacagg acctcgtact
    30061 atcttgcaca atttgcgtaa gacggttgag cgtgcaggtg ttcaggttga aaatgttatc
    30121 atttcaccac tagcaatggt tcaatctgtt ttgaacgaag gggaacgtga atttggtgct
    30181 acagtgattg atatgggggc aggtcaaacg actgtcgcta caatccgtaa tcaagaactc
    30241 cagttcacac atattctcca agaaggtgga gattatgtaa ctaaagatat ctccaaggtt
    30301 ttgaaaacct ctcgcaaatt agcggaaggc ttgaaactga attacgggga agcctatccg
    30361 cctcttgcaa gcaaagaaac cttccaagta gaggttattg gagaagtaga agcagtcgaa
    30421 gtgacggaag cctacttgtc agaaattatt tctgcacgaa tcaagcacat ccttgaacaa
    30481 atcaagcaag aattagatag aaggcgtcta ttggacctcc ctggtggtat tgtcttaatc
    30541 ggtgggaatg ccattttacc aggtatggtt gagcttgctc aggaagtctt tggcgtccgt
    30601 gtcaagcttt atgttccaaa tcaagttggt atccgtaatc cagcctttgc gcatgtgatt
    30661 agtttatcag aatttgcggg tcaattaaca gaagttaatc ttttggctca gggagcgata
    30721 aaaggtgaga atgacttaag tcatcagcca attagttttg gtgggatgct gcaaaaaaca
    30781 gctcagtttg tacaatcaac gcctgttcaa ccagctcctg ctccagaagt agagtcggtg
    30841 gcgcctacag aaccaatggc ggatttccaa caagcttcac aaaataaacc gaaattagca
    30901 gatcgtttcc gtggcttgat cggaagcatg tttgacgaat aaagaggaaa aataaattat
    30961 gacattttca tttgatacag ctgctgctca aggggcagtg attaaagtaa ttggtgtcgg
    31021 tggaggtggt ggcaatgcca tcaaccgtat ggtcgacgaa ggtgttacag gcgtagaatt
    31081 tatcgcagca aacacagatg tacaagcatt gagtagtaca aaagctgaga ctgttattca
    31141 gttgggacct aaattgactc gtggtttggg tgcaggaggt caacctgagg ttggtcgtaa
    31201 agccgctgaa gaaagcgaag aaacactgac ggaagctatt agtggtgccg atatggtctt
    31261 catcactgct ggtatgggag gaggctctgg aactggagct gctcctgtta ttgctcgtat
    31321 cgccaaagat ttaggtgcgc ttacagttgg tgttgtaaca cgtccctttg gttttgaagg
    31381 aagtaagcgt ggacaatttg ctgtagaagg aatcaatcaa cttcgtgagc atgtagacac
    31441 tctattgatt atctcaaaca acaatttgct tgaaattgtt gataagaaaa caccgctttt
    31501 ggaggctctt agcgaagcgg ataacgttct tcgtcaaggt gttcaaggga ttaccgattt
    31561 gattaccaat ccaggattga ttaaccttga ctttgccgat gtgaaaacgg taatggcaaa
    31621 caaagggaat gctcttatgg gtattggtat cggtagtgga gaagaacgtg tggtagaagc
    31681 ggcacgtaag gcaatctatt caccacttct tgaaacaact attgacggtg ctgaggatgt
    31741 tatcgtcaac gttactggtg gtcttgactt aaccttgatt gaggcagaag aggcttcaca
    31801 aattgtgaac caggcagcag gtcaaggagt gaacatctgg ctcggtactt caattgatga
    31861 aagtatgcgt gatgaaattc gtgtaacagt tgttgcaacg ggtgttcgtc aagaccgcgt
    31921 agaaaaggtt gtggctccac aagctagatc tgctactaac taccgtgaga cagtgaaacc
    31981 agctcattca catggctttg atcgtcattt tgatatggca gaaacagctg aattgccaaa
    32041 acaaaatcca cgtcgtttgg aaccaactca ggcatctgct tttggtgatt gggatcttcg
    32101 ccgtgaatcg attgttcgta caacagattc agtcatttct ccagtcgagc gctttgaagc
    32161 cccaatttca caagatgaag atgaattgga tacacctcca tttttcaaaa atcgttaagt
    32221 aaatgaatgt aaaagaaaat acagaacttg tttttcgaga agttgcagag gctagtctga
    32281 gtgctcatcg agagagtggt tcggtctctg tcattgcagt taccaagtat gtagatgtac
    32341 cgacagcgga agccttgctt tcgctaggtg ttcatcatat cggtgaaaat cgtgtagata
    32401 agtttctgga aaaatatgaa gctttaaaag atcgagatgt gacttggcat ttgattggta
    32461 ccttgcaaag acgtaaggtg aaagatgtca ttcaatacgt tgattatttc catgcattgg
    32521 actcagtaaa gctagcaggg gaaattcaaa aaagaagtga ccgagtcatc aagtgtttcc
    32581 ttcaagtaaa tatttctaaa gaagaaagca aacacggttt ttcgagagag gaactgctgg
    32641 aaatcttgcc agagttagcc agactagata agattgaata tgttggttta atgacgatgg
    32701 caccttttga ggctagcagt gagcagttga aagagatttt caaggcggcc caagatttac
    32761 aaagagaaat tcaagagaaa caaattccaa atatgcctat gaccgagtta agtatgggaa
    32821 tgagtcgtga ttataaagaa gcgattcaat tcggttccac ttttgttcgt ataggtacat
    32881 cattttttaa gtaggagaga accatgtctt taaaagatag attcgataga tttatagatt
    32941 attttacgga ggatgaggat tcaagtctcc cttatgaaaa aagagatgag cctgtgttta
    33001 cttcagtaaa ttcttcacag gaaccggctc tcccaatgaa tcaaccttca cagtcggctg
    33061 gtacaaaaga gaacaatatc accagacttc atgcaagaca acaggaattg gcaaatcaga
    33121 gtcagcgtgc aacggataag gtcattatag atgttcgtta tcctagaaaa tatgaggatg
    33181 caacagaaat tgttgattta ttggcaggaa acgaaagtat cttgattgat tttcagtata
    33241 tgacagaggt gcaggctcgt cgttgtttgg actatttgga tggagcttgt catgttttag
    33301 ctggaaattt gaaaaaggta gcttctacca tgtatttgtt gacaccagtg aatgttattg
    33361 taaatgttga agatatccgt ttaccagatg aagatcaaca gggtgagttc ggttttgata
    33421 tgaagcgaaa tagagtacga taatgatttt tttaattcgt atgatttata atgcagtgga
    33481 tatttactcc ctgattttgg tagccttcgc tgtcatgtct tggtttccag gtgcctacga
    33541 atccagttta ggtcgttgga ttgtagcgtt ggtgaaacca gtgcttgctc ccttgcaacg
    33601 cctgccttta cagatagcgg gtcttgattt atctgtttgg gtcgcgattg ttttggttcg
    33661 atttttagga gaaaacctag tgcgttttct ggcgatgata ggatgaataa agggatttat
    33721 cagcatttct ccatagaaga tcgtccattt cttgacaagg gaatggaatg gataaagaag
    33781 gtagaagata gctatgctcc ttttttaact ccttttatca atcctcatca ggagaagcta
    33841 ttaaagattt tggccaaaac ctatggtctt gcttgtagca gtagtgggga attcgtctcg
    33901 agtgagtatg ttcgagtttt attataccca gattatttcc aaccagagtt ttcagatttt
    33961 gaaatatctc tccaggaaat tgtgtattcc aataaatttg aacatttaac gcatgctaag
    34021 attttaggga cagtcatcaa tcaattaggg attgaacgga aactttttgg agatatccta
    34081 gtagatgaag aacgggcgca gattatgatt aatcagcagt ttcttcttct ctttcaagat
    34141 ggactaaaga aaattggtcg tatacctgtt tcgctggagg aacgtccttt caccgagaaa
    34201 atagataagc tagaacagta tcgagaactg gatttatctg tgtctagttt tcgattagat
    34261 gttcttttat caaatgtttt gaaactatct aggaatcaag caaaccagtt gattgaaaag
    34321 aaacttgtcc aagtaaatta tcatgtggta gacaaatcag attacactgt tcaagttgga
    34381 gacttgatta gtgtgagaaa atttggtcgc ttgagattac ttcaagataa gggacaaacg
    34441 aaaaaagaga agaaaaaaat aaccgtccag ttattattaa gtaagtgagg aatagaatgc
    34501 caattacatc attagaaata aaggacaaga cttttggaac tcgattcaga ggttttgatc
    34561 cagaagaagt cgatgaattt ttagatattg tggttcgtga ttacgaagat cttgtgcgtg
    34621 cgaatcatga taaaaatttg cgtattaaga gtttagaaga gcgtttgtct tactttgatg
    34681 aaataaaaga ttcattgagc cagtctgtat tgattgctca ggatacagct gagagagtga
    34741 aacaggcggc gcatgaacgt tcaaacaata tcattcatca agcagagcaa gatgcgcaac
    34801 gcttgttgga agaagctaaa tataaggcaa acgagattct tcgtcaagca actgataatg
    34861 ctaagaaagt cgctgttgaa acagaagaat tgaagaacaa gagccgtgtc ttccaccaac
    34921 gtctcaaatc tacaattgag agtcagttgg ctattgttga atcttcagat tgggaagata
    34981 ttctccgtcc aacagctact tatcttcaaa ccagtgatga agcctttaaa gaagtggtta
    35041 gcgaagtact tggagaaccg attccagctc caattgaaga agaaccaatt gatatgacac
    35101 gtcagttctc tcaagcagaa atggaagaat tacaagctcg tattgaggta gccgataaag
    35161 aattgtctga atttgaagct cagattaaac aggaagtgga aactccaact cctgtagtga
    35221 gtcctcaagt tgaagaagag cctctgctca tccagttggc ccaatgtatg aagaaccaga
    35281 agtagctcca gtgcatccga caggtccaac accagctaca gaaactgttg attcagcacc
    35341 aggatttgaa gcaccgcaag aatccgttac aattttataa gaaacgatgt gagaacaata
    35401 tcttatcctt atatttccag cgagcaggag atggtgtgag tcctgcattc cctaacgata
    35461 agattatcct ctcaaaaact caagtctgaa tctagtaaga tttgacgttt cccacgttac
    35521 gggataagag ggagaaagac taaatctttt tccgaataaa ggtggtacca cgattttcgt
    35581 cctttttgga agtcgtggtt tttaatttgt tattatttat aaaggagata ccatgaaact
    35641 aaaagatacc cttaatcttg ggaaaacagc tttcccaatg cgtgcaggcc ttccgaccaa
    35701 agagccagtt tggcaaaagg aatgggaaga tgcaaaactt taccaacgtc gtcaagaact
    35761 aaatgaaggg aaaccgcatt ttgtcttgca tgatggccct ccatacgcaa acggaaatat
    35821 ccacgttgga catgccatga atcatatctc aaaagatatc atcattcgtt ctaagtctat
    35881 gtcaggtttt aacgcacctt atatcccagg ttgggatact catggtctgc caatcgagca
    35941 agttttggca aagcaaggtg tgaagcgcaa agaaatggac ttggttgagt acttgaaact
    36001 ttgccgtgaa tacgctcttt ctcaagttta taaacaacgt gatgatttca agcgcttggg
    36061 gatgtcaggt gactgggaaa atttatatgt gactttaact ccagactacg aagcagccca
    36121 aatccgtgta tttggtgaga tggctaataa gggttatatc taccgtggtg ctaagccagt
    36181 ttactggtct tggtcatctg agtcagccct tgccgaagcg gaaattgaat accatgattt
    36241 ggtttcaact tccctttact atgccaataa ggtaaaagat ggcaaaggtg ttctagatac
    36301 agatacttat atcgttgttt ggacaactac tccatttact atcacagctt ctcgtggttt
    36361 gactgttggt gcagatattg attacgtttt ggttcaacct gctggtgaaa ctcgtaagtt
    36421 tgtcgttgct gcagaattgt tgactagctt gtctgagaaa tttggctggg ttgatgttca
    36481 agttttggca acttaccgtg gtcaagaact caaccacatc gtaacagaac atccatggga
    36541 tacagctgta gatgaattgg ttatccttgg tgaccacgtt acaactgact ctggtacagg
    36601 tattgtccat acagcccctg gttttggtga ggacgattac aatgttggta ttgctaataa
    36661 tcttgaagtc gcagtgactg ttgatgaacg tggtatcatg atgaagaatg ctggtcctga
    36721 atttgaaggt caattctatg aaaaggtagt tccaactgtt attgaaaaac ttggtaacct
    36781 ccttcttgcc caagaagaaa tctctcactc atatccattt gactggcgta ccaagaaacc
    36841 aattatctgg cgtgcagtac cacagtggtt cgcctctgtt tcaaaattcc gccaagaaat
    36901 cttggatgct attgataaag ttaaattcca tacagaatgg ggtaaagtac gtctatataa
    36961 tatgattcgt gaccgtggtg actgggttat ctctcgtcaa cgtgcttggg gtgttccact
    37021 tccaatcttc tatgcagaag acggtacagc tatcatgaca gctgagacta tcgaatatgt
    37081 ggctcaactc tttgaagtac atggttcaag catttggtgg gaacgtgatg ccaaagacct
    37141 cttgccagaa ggatttacac atccaggttc tccaaatggc gagttcaaaa aagaaactga
    37201 tatcatggac gtttggtttg actcaggttc atcatggaat ggtgttttgg taaatcgtcc
    37261 aaatctgact tatccagctg acctttacct tgaaggttct gaccaatacc gtggttggtt
    37321 caactcatca ctcattacat ctgttgccaa ccatggcgta gcaccttaca aacaaatctt
    37381 gtcacaaggt tttacccttg acggtaaagg tgagaagatg tctaaatctc ttggaaatac
    37441 cattgctcca agcgatgttg aaaaacaatt tggtgctgaa atcttgcgtc tctgggtaac
    37501 aagtgttgac tcaagcaatg acgtgcgtat ctctatggat atcttgagcc aagtctctga
    37561 aacttaccgt aagattcgta atactcttcg tttcttgatt gccaatacat ctgactttaa
    37621 cccaactcaa gatgcagtcg cttacgatga acttcgttca gttgataagt acatgaccat
    37681 tcgctttaac cagcttgtca agaccattcg tgatgcctat gcaaactttg aattcttgac
    37741 aatctacaag gccttggtga actttatcaa cgttgattta tcagcctttt accttgattt
    37801 tgctaaagat gttgtttaca tcgaaggtgc taaatcactg gaacgtcgtc aaatgcagac
    37861 tgtcttctat gatattcttg tcaaaatcac caaactcttg acaccaatcc ttcctcacac
    37921 tgcggaagaa atctggtcat atcttgagtt tgaaacagaa gacttcgtcc aattgtcaga
    37981 attaccagaa gttcaaactt ttgctaacca agaagaaatc ttggatacat gggcagcctt
    38041 catggacttt cgtggacaag cacaaaaagc cttggaagaa gctcgtaatg caaaagttat
    38101 cggtaaatca cttgaagcac acttgacagt ttatccaaat gaagtggtga aaactctact
    38161 cgaagcagta aatagcaatg tggctcaact tttgatcgtg tctgagttga ccatcgcaga
    38221 aggaccagct ccggaagctg cccttagctt cgaagatgta gccttcacag ttgaacgtgc
    38281 tgctggtgaa gtatgtgacc gttgccgtcg tattgaccca acaacagcag aacgtagcta
    38341 ccaggcagtt atctgtgacc actgtgcaag catcgtagaa gaaaactttg cggaagcagt
    38401 cgcagaagga tttgaagaga aataagattg aaaagtctag gcaaaattca atttgagaag
    38461 aaaagacaac taattttata gtctattaaa cgcattgtat cacgtttttg aacacctgat
    38521 atgatgcgtt ttttatttat tttaaaaatt tgcgaggtat gactttttat actcaacaag
    38581 aatcaaagag aaacttagca agctaacagt agtaagataa aataggaatt tgatattagg
    38641 gataagattg gtaaatagtg taatattttt acaagaataa atttatatag ttatttctgg
    38701 tttctgaaaa gtattatatt atattttatt tcatattata caaattttta ttttataata
    38761 tcagaaaata ctttttttaa aaagcaaata tgatacaatt ttatttgaaa aaaaataaaa
    38821 aaggagattt tattatgaaa tcaagtggta tcgtcggtct tgtgggagga attttacttc
    38881 ttatttgtcc ttttgtcttg ttgggaacag cggtaaacac agctgctaca actcttaatg
    38941 gaggagctac tgcaggggct ttttcaggtg ttgccttact cttgaatgcc ttgaagattg
    39001 caaatcttgt tcttggtatc attgctattg tttactataa aggagataag cgtgtaggtg
    39061 cagctccgtc tgtactaatg attgtttctg gtggagttag tctcattcca ttcttaggat
    39121 gggttggggg gattcttgct attatcggag gatctctatt ccttgcaaca ttgaagaaat
    39181 tcaaatcaga agaataaaag gtattttagc atgaaaagaa caaaaagttt atcggtatag
    39241 gagtagctct attatctctt tctcttctag ttgcatgtgg aacataaagt tcaaagaata
    39301 cttcaacaag taatgatgag aagacagtag caacatccaa tagttcaaaa gaaacaatca
    39361 ctttcgatac accggttgta acagacgatg cgattgaatc aatacgcact tatgcagatt
    39421 atatagatct ttataaaaat atttttgatg attattttac taaagctgag gaaggtttca
    39481 aagccatagc tatggaaaat aatgactcgt ttactaaact aaaagagtca actcaaaaat
    39541 tattcgatgc gcagaaaaaa aggttaaata atgaagatag aatagaaaca actaaaaaca
    39601 atgtgattgc caaacattgt caaacagtcc tttccttttt ggttttgact agcttttttg
    39661 tgaaaaattg tgtaaaatag aatagataaa cgaggggaaa cctcggaaaa tttaaaggag
    39721 aatccatcta atggtaaaat tggtttttgc tcgccacggt gagtctgaat ggaacaaagc
    39781 taaccttttc actggttggg ctgatgttga tttgtctgaa aaaggtacac aacaagcgat
    39841 tgacgctggt aaattgatca aagaagctgg tatcgaattt gaccaagctt acacttcagt
    39901 attgaaacgt gctatcaaaa caactaactt ggctcttgaa gcttctgacc aattatgggt
    39961 tccagttgaa aaatcatggc gcttgaacga acgtcactac ggtggtttga ctggtaaaaa
    40021 caaagctgaa gctgctgaac aatttggtga tgagcaagtt cacatctggc gtcgttcata
    40081 cgatgtattg cctccaaata tggaccgtga tgatgagcac tcagctcaca cagaccgtcg
    40141 ttatgcttca cttgacgact cagtaatccc agatgctgaa aacttgaaag tgactttgga
    40201 acgtgctctt ccattctggg aagataaaat cgctccagct cttaaagatg gtaaaaacgt
    40261 attcgtagga gctcacggta actcaatccg tgcccttgta aaacacatca aaggtttgtc
    40321 agatgatgag atcatggacg tggaaatccc taacttccca ccattggtat tcgaattcga
    40381 tgaaaaattg aacgttgttt ctgaatacta ccttggaaaa taaaaaattg taagcctagg
    40441 attgatttct aggcttttta tgttagtatg gaagtatgat aaggaataaa aaacaagatt
    40501 atgtactggc ctacaagcaa ccagcttcaa ccacttacat gagttgggaa gaagaagctt
    40561 taccgatagg taatggttct ttaggagcaa aagtatttgg ccttataggg gctgaacgga
    40621 ttcaatttaa tgaaaaaagt ctctggtctg gaggtccact tcctgatagt tcagattatc
    40681 agggtggaaa tcttcaggat cagtatgttt ttttagctga gattcggcag gctttggaga
    40741 agagagatta caatctggct aaggaactgg ctgagcagca cctaattggg ccaaaaacga
    40801 gtcaatatgg gacctatctg tcttttgggg atattcacat tgagttcagc cagcaaggta
    40861 cgactttgtc tcaggtgacg gactatcaga gacagctgaa tattagtaag gcacttgtga
    40921 cgacttctta tgtctataag ggaacgcgat ttgaacgtga agcttttgcg agttttccag
    40981 atgatctctt ggttcagcgt tttactaagg aaggggcgga aactctagat tttactatag
    41041 aactatcctt gacctgtgat ttggcttctg atgaaaagta tgagcaggaa aaatctgatt
    41101 acaaggagtg taagttggat attactgatt ctcatatctt gatgaaggga agagttaagg
    41161 ataatgatct gcggtttgct agttatctag cttgggaaac ggatggagat attagagttt
    41221 ggtcagatag ggttcagata tcaggagcca gttatgccaa tctcttcttg gccgctaaga
    41281 cggattttgc ccaaaatcct gctagcaatt atcgcaagaa actagattta gagcaacagg
    41341 tgatagactt ggtggacaca gctaaagaaa agggctatac ccaattgaaa tcaaggcata
    41401 tcgaggacta ccaagcctta ttccagcgtg ttcaattgga tttggaagct gatgttgacg
    41461 catccactac agatgatttg ttaaaaaatt ataagccaca agaagggcag gctttggagg
    41521 agctgttctt ccagtatgga cggtatttat tgattagttc gtccagagac tgcccagatg
    41581 ctctaccagc taacctacag ggagtctgga atgcagtcga caatcctcct tggaattcgg
    41641 actatcactt aaatgtcaat ctgcagatga attattggcc agcctatgtt accaatctcc
    41701 tagaggcggt ctttccggtc atcaactatg tagatgattt gcgtgtctat ggtcgtctag
    41761 cggctgtaaa gtatgcagga atcgtctctc aaaaaggtga ggaaaatggt tggttggttc
    41821 atactcaagc gactcccttt ggttggacgg cacctggttg ggattactat tggggttggt
    41881 caccagctgc caatgcgtgg atgatgcaaa ccgtttatga agcctattca ttttataggg
    41941 accaagacta tctcagagag aaaatttatc ccatgttgag ggaaacggtt cgtttttgga
    42001 atgccttttt acataaggat cagcaggcgc agcgttgggt ctcttctccg tcttattccc
    42061 cagaacatgg gccgatttcg attggcaata cctatgacca atctctgatt tggcagttat
    42121 ttcatgattt tattcaggct gctcaggaat tgggactgga tgaggacttg ttgactgagg
    42181 ttaaggagaa gtctgattta ctaaatcctt tgcaaatcac tcaatctggt cgaatcaggg
    42241 agtggtatga ggaggaagag cagtattttc aaaatgagaa agtggaggcc cagcatcggc
    42301 acgcttccca tctagtggga ctctattctg gcaatctctt tagctacaag ggacaagagt
    42361 atattgaagc ggcgcgtgct agcctcaatg atcgtggaga tggcggcaca ggctggtcca
    42421 aggctaataa gatcaatctc tgggcgcgtt tgggagatgg caatcgagcc cataaattat
    42481 tggcagagca gttaaagaca tccaccttgc caaatctttg gtgtagccat cctccttttc
    42541 agatagatgg taattttggt gctactagtg gcatggcaga aatgttactc cagtctcatg
    42601 cagcttatct ggtacctcta gctgccctac ctgatgcttg gtcaacaggt tctgtttcag
    42661 gcttaatggc acgtggacat tttgaagtga gcatgagctg ggaagataaa aaactcttac
    42721 agttgaccat tttatcaagg agtggaggag atttgcgagt ttcttatcca gatattgaga
    42781 agagtgtgat taaaatgaat caagaaaaaa taaaagcgaa atgcatgggg aaagattgta
    42841 tttcggtggc aacagcagaa ggtgatcttg ttcaatttta tttttaagaa gatgttataa
    42901 ggcagtaatt tgaaactgcc ttttaataag gatttaagaa tataagcagt tttcaactag
    42961 ttgaaaaagc gttataatga taataggaag taatactcaa tgaaaatcaa agagcacaaa
    43021 caaggaagct agccgcaggt tgctcaaaac agtgttttga ggttgcagat ggaagctgac
    43081 gtggtttgaa gagagatttt cgaggagtat aatttgtttg atagagggtg ggtctgatgg
    43141 cttatattga gatgaaacac tgttacaagc gttatcaggt tggggacacg gagattgtgg
    43201 ccaatcgtga tgtgaatttt gagattgaaa agggggagct ggttattatc cttggtgctt
    43261 caggtgcagg caagtcaaca gtgcttaacc ttcttggggg aatggatacc aatgatgaag
    43321 gggaaatctg gattgatggt gttaatattg cggattatag ttcccaccag cgcaccaatt
    43381 accgtagaaa tgatgtgggg tttgtttttc agttttataa tctagtttct aatctgacag
    43441 ctaaggaaaa tgtggaactg gcttctgaaa ttgtgacaga tgccttgaat cctgatcagg
    43501 tcttgacaga tgtaggtctg gctcatcgtc taaataactt tccagcccag ctttctggag
    43561 gggagcaaca gcgagtctcc attgcacgcg cggtagccaa aaatcctaaa attctccttt
    43621 gtgatgaacc gactggagcc ttggattatc agacgggcaa gcaggttttg aaaattctcc
    43681 aagatatgtc tcgtcaaaag ggagcgacgg tgatcatcgt gactcataat ggagctttgg
    43741 cgcccattgc tgatcgcgtg attcatatgc acgatgccag tgtcaaggat gtggtgctca
    43801 accagcatcc tcaggatatt gacagtttgg agtactagta tgatcaagcg aaaaacttat
    43861 tggaaggact taattcagtc cttcacaggc tccaaggggc gttttttatc catcttgatc
    43921 ctgatgatgt tgggatctct agccttagta ggtctcaaag taaccagtcc caacatggag
    43981 gcgacagcta atgcttattt aacaactgct caaaccttgg atttggcagt catgtctaac
    44041 tatggcttgg atcaagcaga ccaagaagaa ctaaaacaga cggagggcgc agaggtcgag
    44101 tttggctatt tgacagatgt gactatggat aatgggcagg atgccattcg gctgtactcc
    44161 aaaccagagc gaatttcaac ctttcagcta agaaagggac gacttcctca gtcagacaag
    44221 gaaatcgctt tggccactca tttgcaaggc caatacagcg tgggacagga gattagtttt
    44281 aaagaaaaag aagagggtca ttcctcttta aaagaccata cttataccat tactggtttt
    44341 gtggattcgg ctgaaatcct ctcccagcga gatatgggct acgcaggaag tggaagtggg
    44401 actctgacag cctatggggt gattttacct agtcagtttg atcagaaagt ctacaatata
    44461 gctcgtttga aatatcaaga tttagcaggt ttaaatgcct tttcatcagc ttatgaagaa
    44521 aaatccaagc aacatcaaga agagcttgaa caaattttat cagataatgg caaggtacgt
    44581 ctgcaacttt tgaaaaaaga aggacaagag tctctagaca aggggcaaga gacccttgac
    44641 aaggctcaga ctaatttgca ggaaggcaag cgtcgtttag cagctgctca agctcgtata
    44701 caggctcaag aaagtcaact agccttgttt cctcaagttc agagagagca ggctagtgct
    44761 caacttaccc aagccaagca ggaattgggc aaggaagagg acaaactaaa gcaagctgaa
    44821 caaaatctag cccaagaaaa ggaaaaatta gaaaaacatc agcaagtctt ggacgatttg
    44881 gcggagccaa ggtatcaggt ctataatcgt cagaccatgc caggtggtca gtgctatctt
    44941 atgtatagca atgcttcatc cagtattcga gcagtgggca atatctttcc tgtggtactt
    45001 tatgccgtag cagccatggt gacctttacg accatgactc gctttgtaga cgaagagcga
    45061 actcatgcag ggatttttaa ggccttgggt taccgtagta aggatattat cgccaagttt
    45121 ctcctttatg gactagtagc tgggactgtt ggaacggctc taggtagtat acttggtcat
    45181 tatttgctag ccagtgtaat ttcaagtgtc attacaaaag gcatggtggt gggagaaacc
    45241 cagattcagt tctattggac ctatagctta ctagctcttg tcttgagctg gttggcgagt
    45301 gtgttaccag cctatctggt ggcttggagg gaacttcatg acgaagcagc ccagcttcta
    45361 cttcctaaac ctcctgtcaa aggagctaaa atcttattgg agcgtatcgg ttttatctgg
    45421 cgtcgtctca gttttactca taaggtaaca gcccgcaaca tctttcgtta taagcagaga
    45481 atgttgatga caatctttgg tgtggcaggt tctgtagctc tgctctttgc aggtttggga
    45541 atccaatctt ctgtagcagg agttccgtct aaacagtttc aacaaatcca acagtatcag
    45601 atgcttgtct ctgaaaatcc tagtgcgacc aatcaggaca aggtagagct agcagaagtg
    45661 ttgaaagggc aggagatact agcctaccag aaaatctatt ctaaaacgct agacaaggat
    45721 ttcaaaggca aggctggtct tcaaaacatt actcttatga tgatagagaa ggaagatttg
    45781 actcccttta tccatcttca acatcatcag caggagctga cattaaaaga tggcatcgtt
    45841 attacagcta aactcgccca gctggcaggt gtcaaggttg ggcagacttt agaaattgaa
    45901 ggtaaggaac taaaggtcgc tgctattact gagaactacg ttggtcactt tatttatatg
    45961 agtcaggcta gctatgagca actttacgga cagctacccc aagccaacac ttatctggtc
    46021 tcattaaggg ataccagtgc aactagtatc gaaagtcagg cgggcttgct tatgaatcaa
    46081 tctgcggtgt ccagcgttgt ccaaaatgct tcagccattc gactcttcga ctctatcgct
    46141 agctcactca atcagaccat gaccatcttg gtcatcgtat cggttctatt agctattgtc
    46201 atcctttaca atctgaccaa tatcaacgta gctgagagaa tccgtgaact ctccactatc
    46261 aaggttctcg gttttcataa taatgaagtc accctctaca tttaccgtga gacgattgtg
    46321 ctgtcccttg tgggaatcgt acttggtctg gtagctggtt tctatttaca ccaatttttg
    46381 attcaaatga tttcgcctgc gactattctc ttttatccgc aggtaggctg ggaagtctat
    46441 gtaatcccag tggcaacagt aagcatcatt ttgaccttgc ttggtttctt cgtcaattat
    46501 tatttgagaa aggttgatat gttagaagcc ctgaaatctg tagagtaagg tagttatttt
    46561 tagctgattg aacttctatt tactaatatt caaaaatcct ccgtttcaaa gagcagggaa
    46621 ctctttgtga cagaggattt tttctatagg gctttagcag ctgcaattgc ggcttcgaag
    46681 tttggctcag aattgatatt atccacgtat tcaacgtagc gaatcgtatt gtcagtatcg
    46741 aggacaaaga ctgcgcgtgc taataggtgc cattcgttga tcaagagggc ataatcgcgc
    46801 ccgaaagaat ggtcaaagta gtctgaaagc ataatggcat tgtcaaggcc ttcagcaccg
    46861 caccaacgtt tttgagcaaa aggtaggtcc attgaaacag tcaatacgac cgtgttgtcc
    46921 agtccagcca attcttcatt aaaacgacgt gtttgagttg agcagatgcc tgtatcgata
    46981 gaaggaacga cactcaagac ttttttcttg ccatcaaaat cagccagaga ttttttagaa
    47041 agatctgttg tagtaagaga aaaatcaagc gccttgtcgc cgacttgtag ttgtttacct
    47101 gtaaagctta caggatttcc gagaaaagtt accataggat actccaatct tttttcttcc
    47161 attgtatctg aaacagtcag aattttccaa tgatttgacc ggaaatgtgg gcatagaaaa
    47221 aacgccagct cacatgagaa tgacgttttt cagaggctta ttttgccaat ccttcagcaa
    47281 tcttgtcaag gttgtatttc atcatgctgt agtagctgtc gccttcttta ccttgttctg
    47341 cgatagaatc agtaaagatt tgtgcgtaga ttgggatgtt tgtgtcttga gaaacagttt
    47401 tcattggacg gtcatccaca cttgattcta caaagagtga tggaactttt gtttggcgaa
    47461 gtttttcaac caaggtcttg atttgttcag gagttccctc ttcttcagta ttgatttccc
    47521 agatgtaggc acttggaaca ccataggctt tagagaagta tttgaatgct ccttcgctgg
    47581 ttacaatgag tttcttttca gcagggatct tattaaattt atccttactt tctttatcaa
    47641 gtttgtctaa cttatcagta tattctttga gatttttttc atagaattct ttattgttag
    47701 ggtctttggc gctcaattgt ttggcgatat ttttagcaaa aataataccg ttttcaaggt
    47761 taagccaagc gtgtgggtct tcttttcctt tttcattttg accttcaagg tagataacat
    47821 caacgccgtc gctgactgcg aagtagtctt tgttttcagt tttcttggca ttttctacca
    47881 attttgtaaa ccaagcattg ccacctgttt caaggttgat accgttatag aaaatcaaat
    47941 cagcctcaga agttttctta acgtcttcag gaagtggttc gtattcgtgt gggtcttgcc
    48001 caatcggaac gatactatga aggtcaattt tgtcaccagc aatattttta gtaatatcag
    48061 cgatgattga gtttgtagca acaactttta gtttttgacc agaagttgta tctttttttc
    48121 cgctagcaca tgctacaaga atgattgcag aaagaaagag aacgagtaat gtacctaatt
    48181 ttttcattag atcctccaat ttattagggc tttgcccctt attttaacaa atgtttattt
    48241 ttcagtttca aatatcgttg tttgggagcg ataaagaagc taatgagaaa gaaactagca
    48301 gctgtaagca cgatactaga acctgccgca acattaaaac tatagccaat aaagagtccc
    48361 aaaactgaag cagtagctcc gaaggttgag gaaaggaaaa tcatactttt cagactatta
    48421 gcatacagat aagcagttgc agctggggta atcagcatgg ctacaatcag gatagttccg
    48481 acactttgca tggctgtcac agacacgaga gtcaggagta ccatgagaag gtagtgatag
    48541 aaattgacag gcattcccat ggctttagcc aagagttcat caaaggaagt tatcaagagt
    48601 tgcttgaaga aaatccaaat taacaagagg atggctgccc ccacacccat agtaataaac
    48661 atatccgtat cttggacggc cagaatatta ccaaaaagga tatggaaaag gtcagttgaa
    48721 cttttagcga caccaatcaa gatgataccg agggctaaga aagaagaaaa ggtaatgccg
    48781 atggcggtat cgcttttgat aatcgagttt cctttgatgt aggtaatgat gatggcagct
    48841 agcaatccaa agacaatggc tccgataaag aagtcaaggc ccaagatgaa ggagagggct
    48901 acacctggta agacagcatg tgaaatggca tctcccatga gtgacatccc gcgtagaata
    48961 atgaaacatc ccacagctcc agctacaatc ccgacgacaa tagctgttat caaggcattt
    49021 tgtaggaaat ggaatttttg caatccatcg ataaattctg caatcatagg tcacctccat
    49081 tgaaaaagag ttgattaccg taagcttctt ttagattggt ttcggtaaaa gtttcttttg
    49141 ttggaccaaa ggcaatcact tctcgattga caagtaagac ttgatcgaag tagtggggaa
    49201 tcttgctgag gtcgtggtga acgatgagaa ccgtcttccc agcttttttc aaatctctca
    49261 gcgtattcat gatgatttcc tcactgacag agtcaatccc agcaaagggt tcatccaaga
    49321 ggatatagtc ggcttcctgc accaaacatc tggcaatcaa gacccgctgg aattgacctc
    49381 cagacagttg actaatttga cgttcagcgt agtcagctag gccgacgatt tcaagggcct
    49441 cttgcacttt cttccaatgt ttagccttta aacttcgaaa gagaggaata gagggaaata
    49501 gtcctaacga gacgcattcc ttgaccttga tgggaaagtt gtagtcgata ttgatttttt
    49561 gttcgacata ggcaattcgg tgtaaggatt ttttaacttc cttgtcatcg agaaatgcct
    49621 gaccttgatg tgggataatt cccagcatac cttttaatag tgttgatttc ccagcgccgt
    49681 ttggaccaat gatgccggta attgttggtc catggagcac tagtgaaata tccttaagtg
    49741 ccaacgtttc tttgtaggag acactgaggt tttcgatacg tatcataaac ttgtattcct
    49801 cctgtctctt aatatacatt aaaaaaaaaa ttaagtcaag ttaatttttg aaaaaattaa
    49861 aataataact gaaaaataga ttctaaagat aactttcagg ataaatttct aaattataaa
    49921 acgcatagta tcaagtgtaa aaaacttgga attatgcgtt ttatcatgga aagatttttt
    49981 ataatagcta aaaaataata gttaaatggt tattaattgc attccctagt gatttttgtt
    50041 aagataaatg caaatacaaa tgaaagcgag aacaagatga cacgttatca agatgatttt
    50101 tatgatgcta tcaatggaga atggcaacag acagctgaaa tcccagcaga taagtctcaa
    50161 acaggaggtt ttgttgattt agaccaggaa attgaagacc tgatgctggc gacaacagac
    50221 aagtggttag caggtgaaga agtgcctgag gatgctatct tggaaaactt tgtcaaatac
    50281 caccgcctag ttcgtgattt tgacaagaga gaagctgacg gtatcacacc tgtcttacca
    50341 ctccttaaag aattccaaga attggagact tttgcggatt ttacagctaa actagcagag
    50401 tttgagcttg caggaaaacc aaacttcctt ccttttggtg tatcgccaga ctttatggat
    50461 gctagaatca atgttctatg ggctagcgct ccaagcacaa tcttgccaga tacgacctac
    50521 tatgcagaag aacatcctca gcgcgaagag ctcttgactc tttggaaaga aagcagcgca
    50581 aatctcctca aggcttatga tttctctgat gaagaaattg aagacttgct agaaaaaaga
    50641 cttgaattgg accgccgagt tgcggcagtg gtgctctcta atgaagaaag ttcagaatat
    50701 gctaaactct atcatccata ttcttacgaa gatttcaaga aattcgcgcc tgccctacct
    50761 ttggatgact tcttcaaagc agttattggg caattaccag acaaggttat tgtagacgag
    50821 gaacgtttct ggcaagcagc agagcaattc tacagtgagg aagcctggtc tctccttaaa
    50881 gcaaccttga ttttgagtgt tgtcaatctt tcaaccagct atttaacaga ggatatccgt
    50941 gttttgtctg gtgcctacag ccgtgccctt tctggagttc cagaggcaaa agataaggtc
    51001 aaagcagctt atcatctagc acaagaacct ttcaagcaag ccctgggtct ttggtacgcc
    51061 cgtgagaagt tctctccaga agccaaggcg gatgtggaga aaaaagtggc aaccatgatt
    51121 gatgtttata aggagcgtct gcttaagaat gactggctca ctccagaaac ctgtaaacag
    51181 gctatcgtga agctcaatgt gatcaaacct tatattggct atccagaaga attgcctgca
    51241 cgttacaagg ataaggtagt gaatgaaact gccagtcttt ttgagaatgc tctagccttt
    51301 gcgcgtgtgg aaatcaagca cagttggagt aagtggaacc agcctgtaga ttataaggaa
    51361 tggggcatgc ctgctcatat ggtcaatgcc tactacaatc ctcagaagaa cctgattgtc
    51421 tttccagcgg ccattttaca ggcgcctttc tatgacttgc atcagtcatc ttctgctaac
    51481 tacggtggta ttggggcagt gattgcccat gaaatttccc acgcctttga tactaacggg
    51541 gcttcctttg acgaaaatgg tagcctcaag gattggtgga cagagagcga ctatgctgcc
    51601 ttcaaggaga aaacacaaaa agtcattgac caatttgatg gacaggattc ttatggagca
    51661 accattaacg gtaaattgac tgtatcagaa aacgtggctg acttgggagg aatcgcagca
    51721 gcgcttgaag cagctaagag agaagcagac ttctcagcag aagagttctt ctacaacttc
    51781 ggtcgcatct ggcgcatgaa aggtcgacca gaatttatga aacttttggc tagcgtcgat
    51841 gtgcacgcac cagccaaact ccgtgtcaat gtgcaagtac caaacttcga cgatttcttt
    51901 acaacctatg atgtcaaaga aggagacgga atgtggcgtt caccagagga gcgcgtgatt
    51961 atttggtaat gcaaaaaatc tagtcatcag aaaaaaggca tccaatcatg tgtgaaaacc
    52021 ttgataggat gccttttgta atagaaagat ttgctggata gtttacttaa atttcgattg
    52081 tcatcatctt gctagaaaaa gggattgaag gccttttcgt gagcgatagt agtagctgga
    52141 ccatgtcctg gatagacatc gtagtttggt agggtgaaga gttgggtctg gatactatga
    52201 aggagttgct ccatgctacc agtcggaagg tcggtccgtc cgatagtttc gcggaataga
    52261 gcatctcccg tcaagactag atgagcatca ggaaagacta gggaaacacc accgatagag
    52321 tgccctgggg tcggtagaac cttaaaacga aattcctcta gttggtattc ttcgtgaaag
    52381 acaaaggtgt gttctgcagg ttttgtgacc acatctgcca tatcatcgtg gcgagggaga
    52441 ccggagagat tatcgacagg agtgtagagc cagctggctt cgctctctgc gatatagaca
    52501 ggaggattgc caaacgtctc gcgaaccaag tccagactca tgatatggtc ataatgggcg
    52561 tgggtcaaga gaatagcaca gatcgttttg ttgatcttct cgattgtctg acgaatggct
    52621 tcccaatgac taccaggatc gacgacgatg aggtgctttt cgccttctag ataataggta
    52681 ttttcatagg caacaggatt cacggtttta tggattttca tactagctcc aatctcaaag
    52741 aatgtactaa tactcttcga aaatctcttc aaaccgcgtc aactttatct gcaacctcaa
    52801 agcagtgctt tgaacagcct gcggctagct ttctagtttg ctctttgatt ttcattgagt
    52861 ataaattaag tatagcacag ttagggagaa taggtaagga tttaaaatga acttaaaaac
    52921 cagcatgact agatgaagac aggctggtta tcaatttatc aatattggaa aggtgtcaat
    52981 tgcccttcat aggttgcata aactccgtca ttagcttgtt tgatagacgt gatgtttaga
    53041 gtgctaccgt agttggcttc tcatttgtcg ctatattcac tataagttat cctactgggc
    53101 aggttctggt cgttagcata gtattgaacg actgtttttt atagctttgc tggctgaata
    53161 ggaacaggca gcaaactcct aacattagga gagcaaagat agaaatagca gtttttttca
    53221 taggtattct ccttataaac taaaggatag aagaaagtat cttgaggatt tttatactct
    53281 tcaaaaatct cttcaaaccg cgtcaacttt atctgcaacc tcaaagcggt gctttgagta
    53341 acctgcggct agtttcctag tttgctcttt gattttcatt tagtattaat tccaaaatcc
    53401 agtcaaggta ccagtgtatg tgatacgagt tccaccgttt atctttcgta tcttcaatgg
    53461 aattggggaa gtcagaaagt ctttgggtta caatatggtt tccgttcttc tatctctcct
    53521 tctatcacct aagtttaaaa tctaacttcg tagtatatta cggaccaata attcagaatt
    53581 atcaagcaat tttgaattat ttttttctta ttctgttcca atctagtttt catattctta
    53641 aaaaagtgat aaaatggtag gaagaattgg agagtagaga tgccgaaaga agtgaattta
    53701 acaggcgaag aagttgtcgc tttaaccaaa gaatatttaa cggaagagga tgttcatttt
    53761 gtccataagg ccttggtcta tgctgttgaa tgccacagtg gtcaatatcg caaatcaggc
    53821 gagccttata tcattcaccc tatccaagtg gcaggtattt tagctaagct aaagctggat
    53881 gctgtaacag tagcttgtgg attcttgcat gatgtggtgg aagatacaga tgcgactttg
    53941 gacgatttgg aaagagagtt tggtcctgat gtgcggatga ttgttgacgg agttaccaag
    54001 cttggcaagg tcgagtacaa atcgatcgag gagcaattag cggaaaatca tcgcaagatg
    54061 ctcatggcca tgtctgagga catccgcgtt attttggtca aactgtctga ccgcttgcac
    54121 aatatgcgga ccctgaaaca tcttcgaaaa gacaagcagg agcgtatttc caaagaaacc
    54181 atggaaatct atgccccgct tgcccatcgt ttggggattt ccagtgtcaa atgggaatta
    54241 gaagacttgt ctttccgtta tctcaatcca acggagtttt acaagattac ccatatgatg
    54301 aaggaaaagc gcagggagcg tgaggccttg gtggatgagg tagtcacaaa attagaggag
    54361 tatacgacag aacgtcactt gaaagggaag atttatggtc gtcccaagca tatttactca
    54421 attttccgca aaatgcagga caagagaaaa cggtttgagg aaatctatga tctgattgct
    54481 attcgttgta ttttagatac ccaaagtgat gtttatgcca tgcttggtta cgtgcatgaa
    54541 ttttggaaac cgatgccagg tcgcttcaaa gactatatcg ccaaccgcaa ggccaatggt
    54601 tatcagtcta tccatacgac tgtttatgga ccaaaagggc cgattgaatt ccagattcga
    54661 accaaggaaa tgcacgaggt ggctgagtac ggggttgcgg ctcactgggc ttataagaaa
    54721 ggtataaagg ggcaagttaa cagcaaggaa tcagctattg gaatgaactg gatcaaggag
    54781 atgatggagc tccaagacca ggctgatgat gctaaggaat ttgtggactc tgttaaggaa
    54841 aactatttgg ctgaggagat ttacgttttt accccagatg gagctgtccg ttcccttccc
    54901 aaagattcag gaccgattga ttttgcctac gaaatccata ccaaggtcgg tgaaaaagca
    54961 actggtgcca aggtcaatgg ccgcatggtt ccactgacaa ccaagttaaa gacaggggat
    55021 caggttgaaa ttatcgccaa cccgaactcc tttggaccta gccgtgactg gctcaatatg
    55081 gtcaagacta gcaaggcgcg caataagatt cgccagttct ttaaaaacca agataaggaa
    55141 ttgtctgtca acaagggtcg tgagatgctg atggctcagt tccaagaaaa tggctatgtg
    55201 gcaaataaat ttatggacaa gcgccacatg gatcaagttc tgcaaaagac cagttacaag
    55261 acagaagact ccctctttgc ggccattggt tttggggaaa tcggtgcgat taccgtcttt
    55321 aaccgtctga ctgaaaagga acgccgtgag gaagagcgtg ccaaggccaa agctgaggca
    55381 gaggagcttg tcaaaggtgg cgaggtcaag gttgaaaata aagaaaccct caaggtcaag
    55441 catgaggggg gagtggttat tgaaggtgct tctggtctcc tagtgcggat tgctaagtgt
    55501 tgtaaccccg tgcctggtga cgatattgtt ggctacatta ccaagggtcg tggtgtggct
    55561 attcaccgtg tggactgtat gaacctgcgt gcccaagaaa actacgagca acgtctcctt
    55621 gatgtggaat gggaagacca gtaccctagc tcaaataagg agtatatggc ccatatcgat
    55681 atctacggtc tcaaccgtac aggactgttg aacgatgtac tgcaagttct ttcaaataca
    55741 accaagaata tttcaacggt caatgcccaa ccaaccaagg atatgaagtt tgctaatatc
    55801 catgtgtcct tcggtattgc caacctctct acactgacca cggttgtcga taaaattaag
    55861 agtgtgccag aagtttactc tgtcaaacgg accaacggct agttgtccta gctcttacta
    55921 gaaaggctat tatgaaaatc attatccaac gggttaaaaa agcccaagtg agtatagaag
    55981 gccagattca gggaaaaatc aatcagggac ttttattgct ggttggtgtt ggaccagagg
    56041 accaagagga agatttggac tatgctgtga gaaaactggt caatatgcgg attttttcag
    56101 acgcagaagg caagatgaac ctgtctgtca aagatattga aggagaaatc ctctctattt
    56161 ctcagtttac cctctttgcg gatactaaga gaggcaatcg tccagccttt acaggggcag
    56221 ctaaacctga tatggcatca gacttctatg atgctttcaa tcaaaaatta gcgcaagaag
    56281 tgcccgttca gacaggcatc tttggagcag atatgcaggt tgagctggtt aataatggac
    56341 ctgttaccat tatcctagat actaaaaaga gataagaaag accaagccca gtcggcttgg
    56401 tctttctcat ctatcataaa atactccaaa aagaaatcgg ttcttgatat gcttggggga
    56461 agtctctttt caggctttgg cagatgcgat aggaagggat gagatgtcct aggatgagga
    56521 gagttccctg aaggaaaagt ggaatatcac ttaaagacac caaggagaga atcatcaggc
    56581 tatcccatag gagaatttgt aaagcaaaac gccagaatct cggtctaatc tgggaataga
    56641 gttaactaaa gtggtcacaa agttggtttg aaagataggt caatagcaca ggatgaaaga
    56701 aaatgagcca accgctataa atcaaaatcc agtcccaagt aagccccctt ttaaatgtca
    56761 aaaatgccag catgattcca tcaatgacaa ggagttgaat ggttttgatg aagatgattt
    56821 ttttcatgct aaaacctcct tttccttaga gacactccca taaagagatg tgtttgtcgt
    56881 aaaagtctgg atagttttct cttaggtagt cagctgtcat ataataaaaa gtagaatggc
    56941 aagcagtgat gattggcata agagatgaaa aacgctgaga actaatgaag gctaagagaa
    57001 caaaaaatag aaaatcaatg gtgagaaccc ctaaatagta gaagcgaatc tttttattga
    57061 tttgaggata aatctcagcg aattgatgtt tgagtcggac gaccaagaga aatagcagga
    57121 gtccttgagc aaggaagaaa attaaaccag agagcaagag ccactcaaaa gatgtctcga
    57181 gtctagtgat aaaaaatgca aatagtagca aatcaataac tgcaattgct ataaaatgga
    57241 ttctatagag ttttttcatg acaagacctc cctcttttat ctaacttcat tctactccaa
    57301 aagaatggga gttacaacta aaatgataaa aatagcagaa gggagattct cttaagttgg
    57361 ctagtattct ttatttgagt ttccttctat tatctaactt cttcatcatt ccagacaaat
    57421 aaagctccga ttgcattgag gatataaaag atgtatttac cgatattggc gaagtttcct
    57481 tgaataccag cttttgtcag ctgaacgaaa ttgtaaatca accaaaagcc ccactgagtt
    57541 gttagtttta atgcattcaa agcattggca atgagggaca gtgcaaaggc aatagttgtt
    57601 acgtaggcaa ggagattcat cttgccccca tatccgatat agttggtcac aaaggcaaag
    57661 aggaaggcga tgatggaaat gatgatggcc gccaatttta cctgtttttg gctcatttgg
    57721 ttgggtctgc cttcttgcga agcttcccac ttctttatag caaaggtata aatgaggaag
    57781 gtgacgggat aggtaatgat ggccgcctta tttccaagga tataatcaat agcaccggac
    57841 aaaatggtat taacaatacc aaagtaattt ccccatttgc ttaatttccc cgtgaaacga
    57901 gtggacaaca tggaaatccc aacgttggtt acggaaatca atccaaaggg tacaagagct
    57961 gtccatgatc cccagtctac aaatttatcg aggtgtgagt tgaggtaacc agatgcaatc
    58021 gcaatcccaa cgaccaaagc aaccccgaag aggtcaaact atttagatgt agcaaaaatt
    58081 tttagtgatt ttttcatagg ttaaactacc tttctttttt tcaaatattc tcccaccaaa
    58141 tgaaagtaaa ataaaatgat agaaataaaa ccctgaaaat aa
//
LOCUS       DAVDWT010000010        54743 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVDWT010000010 DAVDWT010000000
VERSION     DAVDWT010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 54743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54743
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.010"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            145..6846
                     /locus_tag="VUW50_000722"
     CDS             145..6846
                     /locus_tag="VUW50_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET7982666.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTTEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIENRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLYKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDKWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAKNAAVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKSLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSNLVAEVTVRVTDKLGETLSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDYSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQGKVQFFA
                     DSGTDAPSKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAIKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSFPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKIEFVEKDLTYKTVEKKDSTLYLGETRVEQEGKVGK
                     ERIFTAINPDGSKEEKLREVVEVPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(7094..7950)
                     /locus_tag="VUW50_000723"
                     /pseudo
     CDS             complement(7094..7950)
                     /locus_tag="VUW50_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8162..8521)
                     /locus_tag="VUW50_000724"
     CDS             complement(8162..8521)
                     /locus_tag="VUW50_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HET7982667.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            8613..9569
                     /locus_tag="VUW50_000725"
                     /pseudo
     CDS             8613..9569
                     /locus_tag="VUW50_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            9553..10110
                     /locus_tag="VUW50_000726"
     CDS             9553..10110
                     /locus_tag="VUW50_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7982668.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERYAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            10103..10357
                     /locus_tag="VUW50_000727"
     CDS             10103..10357
                     /locus_tag="VUW50_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982669.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            10369..12423
                     /locus_tag="VUW50_000728"
     CDS             10369..12423
                     /locus_tag="VUW50_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET7982670.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLGNTEMIIESLENLKGVYRRSLISFMQESRLRETTIISSGAFVERVINRVKPNNIN
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            12602..13462
                     /locus_tag="VUW50_000729"
     CDS             12602..13462
                     /locus_tag="VUW50_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET7982671.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSINFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQFLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAVV
                     LLVILGVLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            13673..14383
                     /gene="ccdA2"
                     /locus_tag="VUW50_000730"
     CDS             13673..14383
                     /gene="ccdA2"
                     /locus_tag="VUW50_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET7982672.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIIFLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            14403..14969
                     /locus_tag="VUW50_000731"
     CDS             14403..14969
                     /locus_tag="VUW50_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HET7982673.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            14980..16092
                     /gene="msrB"
                     /locus_tag="VUW50_000732"
     CDS             14980..16092
                     /gene="msrB"
                     /locus_tag="VUW50_000732"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET7982674.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            16360..17097
                     /locus_tag="VUW50_000733"
     CDS             16360..17097
                     /locus_tag="VUW50_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7982675.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            17094..18785
                     /locus_tag="VUW50_000734"
     CDS             17094..18785
                     /locus_tag="VUW50_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7982676.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIDTGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYHMKIDSRPQKGTKVEIYINRIETS"
     gene            18901..19464
                     /locus_tag="VUW50_000735"
     CDS             18901..19464
                     /locus_tag="VUW50_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HET7982677.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGHIG
                     KESSQEIGELIVETILDILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKF"
     gene            19486..25395
                     /locus_tag="VUW50_000736"
     CDS             19486..25395
                     /locus_tag="VUW50_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HET7982678.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAAAYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMEGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSITNQEQARTENQVVETEEAPKEEPKSEVKPTDDTL
                     PKVEEGKEDSAEPAPVEEVGGEVESKSEEKVAVKPESQPSDKPAEESKVEQAGEPVAP
                     REDEKAPVEPEKQPEAPEEEKAVEETPKQEDTQPEVVETKDEAANQPVEEPKVETPAV
                     EKQTEPTEEPKVEQVGEPVAPREDEKAPVSPEKQPEAPEEEKTAEETPKQEDKIKGIG
                     TKEPVDKSELNNQIDKASSVSPTDYSTASYNALGPVLETAKGVYASEPVKQPEVNSET
                     KAEKVAANTDAKQSEVNSETASLKTAISGLNTDKVELENQLKIAQGKTETDFSMESWT
                     VLSTAKNKAQEVKDNGTATQEQINEAEKSLKTALADLSVDKTALGSAIDTATKKNKEN
                     YTNQTWAELETVLTAAKSVNTNESKQSEVNEAVEKLTATIEKLVELSEKPRLTLSIEK
                     RDIDRKVTVTYTLENPANTQIKSITATLKKGEEVVKDFVLTEENLKTNHLTALFEKLD
                     YYKEYTLSTDMVYNRGNDDETESISEELIQLNLKKLELKDIQTVSLMKFENGQESQVT
                     HLSDKPTDLSKLYLKVTSSTSKDAVLAVSSIEEEIVENKKIFKIHADTPELVVRKKDG
                     SLSKGFDYYMERVIPHDGDIYYDFKDLISAMTSNPTGTFILGRDISSRNVKPDGNGKS
                     YIKGEFKGKLLGTNDNVRHSIFDLEYPLFDTIKSGVVKDIDFKHVNMVFPDSNQGDNV
                     ATIARVIKDKTKIENVNVEGYLEGRDHVAGLVNNLEGNSEIENISFTGKIKSKGGNSI
                     TAGIAGRNILSRVKRAYVNANIEVLGSTNSSMLVAVNGTTLNASGGWGTWGRLTESVA
                     KGTLEIKRSGQAGGVTATVWPYGAIDKVVSYAKVTKGKELFGSDGDLNNNWFMQKINN
                     IFGVQGISSGDSGNDSKFKRISEEEAKQKVASYNITAPNLMSDSSLLVDRLNESWKNT
                     DQFESIQDYQSQNQLIYQNLTKFTPYYNKEFIVHEGNALTPEQEILKTKKIKSIVGLK
                     GTEFVVDGSDIDTIMLHFEDGSQKRYKVTSTGKFSITNLPEYQVEDLNVVYTSEHIVH
                     PLDSSLINNLVEELKKVELYTESTYQVLGIDKDNANKLNRTKRLFLDESLDAVKTQLP
                     TFVKTMFENEWLHINGESSGAVAALRQKIMDNKTAILLALTYINRYYDVKFSDYNIKK
                     LMLFKPTFHGEKIDLLDRLIRLGSSGENRLKGSENAETFKQLFASETKQKDLVTYLDY
                     NRSLLTNYQTTGEWFKETTKDYIQFEERPSLVEEIKDAKYRVYDNLTAPYYQGYILPL
                     LTLKNTHLAILSNYSTMTFVSREKRPNWKNEDFDKWVKYVATAHRNHVDTWYKILPDN
                     IKGKMVKENVTAVWEGLSIPGSEWVDQNAVDRKGRDYAPAREFFNLVGGPMGGWYAYH
                     GYGAHAGGRNRVNYEVFDVLSEYGISVFTHELTHVNDTWIYLGGYGRRENMGPEAYAQ
                     GLFQSPVPGQPGWGALGLNMAFERKNDGDLIYNASPTQFENRKELDSYMKNYNDTLMM
                     VDYLEGDAVISKGKEAITKWFKKVEPKVVSQTAQYDTVRQLTAEEKEKLSVSSVDDLV
                     DQGLMSDRAVGNNTYNPADFETSYIAIDYMTGIYGGGKNSVGSPGALMFKHNTFRMWG
                     YYGFEEGVLGYASNKFKQASRDEGHAGLSDNFIISKISKGEFLTMEAFKKGYFKKVVE
                     ELKTKGIRPVTINQKTYSTFEELQEGFKQAVERDLKKNQLDERETRNFKFQVFRQLLQ
                     QTDSFKTSIFR"
     gene            25456..27177
                     /gene="pabB"
                     /locus_tag="VUW50_000737"
     CDS             25456..27177
                     /gene="pabB"
                     /locus_tag="VUW50_000737"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HET7982679.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKNLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANQDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            27178..27795
                     /locus_tag="VUW50_000738"
     CDS             27178..27795
                     /locus_tag="VUW50_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7982680.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            27845..28594
                     /locus_tag="VUW50_000739"
     CDS             27845..28594
                     /locus_tag="VUW50_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET7982681.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DIHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTVAQTNTSSATTSGWVEQDGA
                     WYYFDENGNPVKNAWQGNYYLKSDGKMAQGEWIYDSSYQAWYYLTSDGSYAYSTWQGN
                     YYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTASSSNDSNSEYSAALGKAKSYNS
                     LFHMSKKRMYRQLTSDFDKFSNDAAQYAIDH"
     gene            28730..29689
                     /locus_tag="VUW50_000740"
     CDS             28730..29689
                     /locus_tag="VUW50_000740"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HET7982682.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            29786..30625
                     /locus_tag="VUW50_000741"
     CDS             29786..30625
                     /locus_tag="VUW50_000741"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET7982683.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(30673..30843)
                     /locus_tag="VUW50_000742"
     CDS             complement(30673..30843)
                     /locus_tag="VUW50_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HET7982684.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            30964..31848
                     /gene="miaA"
                     /locus_tag="VUW50_000743"
     CDS             30964..31848
                     /gene="miaA"
                     /locus_tag="VUW50_000743"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET7982685.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEGIHNRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVDQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            31841..33079
                     /gene="hflX"
                     /locus_tag="VUW50_000744"
     CDS             31841..33079
                     /gene="hflX"
                     /locus_tag="VUW50_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET7982686.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            33072..33695
                     /locus_tag="VUW50_000745"
     CDS             33072..33695
                     /locus_tag="VUW50_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HET7982687.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            33710..34639
                     /locus_tag="VUW50_000746"
     CDS             33710..34639
                     /locus_tag="VUW50_000746"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET7982688.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            34660..35415
                     /locus_tag="VUW50_000747"
     CDS             34660..35415
                     /locus_tag="VUW50_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7982689.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(35469..36377)
                     /locus_tag="VUW50_000748"
     CDS             complement(35469..36377)
                     /locus_tag="VUW50_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7982690.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            36441..36710
                     /locus_tag="VUW50_000749"
     CDS             36441..36710
                     /locus_tag="VUW50_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HET7982691.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            36710..37090
                     /locus_tag="VUW50_000750"
     CDS             36710..37090
                     /locus_tag="VUW50_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7982692.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            37208..37405
                     /locus_tag="VUW50_000751"
     CDS             37208..37405
                     /locus_tag="VUW50_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982693.1"
                     /translation="MGILSIILGLLFPIVGLILGIIGLVLAISYQKESQLDYKIEKIL
                     NILGIVISVVNWIVAIALIFR"
     gene            complement(37445..38170)
                     /locus_tag="VUW50_000752"
     CDS             complement(37445..38170)
                     /locus_tag="VUW50_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HET7982694.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            38332..40173
                     /gene="typA"
                     /locus_tag="VUW50_000753"
     CDS             38332..40173
                     /gene="typA"
                     /locus_tag="VUW50_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET7982695.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPVEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            40193..40447
                     /locus_tag="VUW50_000754"
     CDS             40193..40447
                     /locus_tag="VUW50_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HET7982696.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            40811..41110
                     /locus_tag="VUW50_000755"
     CDS             40811..41110
                     /locus_tag="VUW50_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET7982697.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSIVSRWDGHSDKGEAPAGKTSYAWIWTKWGEQVAFYCDYD"
     gene            41181..43202
                     /locus_tag="VUW50_000756"
     CDS             41181..43202
                     /locus_tag="VUW50_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET7982698.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREVELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKVGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            43204..43845
                     /locus_tag="VUW50_000757"
     CDS             43204..43845
                     /locus_tag="VUW50_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982699.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            43949..45301
                     /locus_tag="VUW50_000758"
     CDS             43949..45301
                     /locus_tag="VUW50_000758"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET7982700.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQTASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            45305..46363
                     /locus_tag="VUW50_000759"
     CDS             45305..46363
                     /locus_tag="VUW50_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HET7982701.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            46373..47593
                     /gene="divIB"
                     /locus_tag="VUW50_000760"
     CDS             46373..47593
                     /gene="divIB"
                     /locus_tag="VUW50_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HET7982702.1"
                     /translation="MSKDKKNEDKETLEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQKDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVIDMEAGVYSYTVADKLIMEAEEKAKKEAEEAEKRQEEERKRLEEEQKKQEE
                     ESNRNQTTQRSSRR"
     gene            48385..49182
                     /locus_tag="VUW50_000761"
     CDS             48385..49182
                     /locus_tag="VUW50_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50 family protein"
                     /protein_id="HET7982703.1"
                     /translation="MGLIDSMKSRKKINIFKISWDFNITLSEKLNYKRFNKNFEKILF
                     GIFPLALILQCFIMYIYRADILTNFQNFPITLNILLFWLLVVIISATIHEFSHAIIAN
                     NYQIEIPNCGIMLLLFNLAFFVDLSGVQFLKNKKQFLKIMLAGIASNSILSLTGLLVL
                     LFTSYKLLGLLFFIINLGMIFINSIPFFQYDSGIILRYLNSNNNNLNFQYIVQLLIAF
                     VIIYFPLSQLLQFLTPNIIVRSIGGVVVSILLSILYMRGRTKYVLRK"
     gene            49166..49354
                     /locus_tag="VUW50_000762"
                     /pseudo
     CDS             49166..49354
                     /locus_tag="VUW50_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828969.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
     gene            49391..50539
                     /locus_tag="VUW50_000763"
     CDS             49391..50539
                     /locus_tag="VUW50_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET7982704.1"
                     /translation="MDSRYSRTKGYYSLFYNEEYNKIQNKTVLVLGAGALGCYISLSL
                     SMYGVRKLIVADYDIIEPSNLNRQILYTESDVGKEKINVLSQKIHKYNSDVQVVPISI
                     KVSSVEELENIVAEYGSIDFIVKAIDTPIDIIKIVNQFAVSHKISYISGGFNGCYLII
                     DNIYIPTIGSCFACRNINKDINKYTLSDKTKWPTTPEMPAILGGIMTNLIIKIFLGCY
                     NEILIDNAYVYNMRNHALSQEKYVLENGECPICKKNNKVKDNNIRAKTFIRSVCFCLL
                     SGGVAFLSAIGQFTVIETQLIVLFLGIIFAIYYAYYNKNIQTSLENIVWLFSSFEILF
                     LLVNFRTFIQLPVDIFISMIIFLMLWIFIMLGIVCLSYYITLLFSKEA"
     gene            50749..51582
                     /locus_tag="VUW50_000764"
     CDS             50749..51582
                     /locus_tag="VUW50_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982705.1"
                     /translation="MIEVSHLSKSFGDKIALNDISFTVKEGQIFGFLGPSGSGKTTTI
                     NILTGQLLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYEKMSLYNNLLFYSKFY
                     NISKLRVDNLLKRVGLYDSCKMVAGKLSTGMRQRMLLARALINKPAVLFLDEPTSGLD
                     PTTSRTIHELILELKTAGTTIFLTTHDMNEATLLCDYVALLNKGKLVEQGAPSELIQR
                     YNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLEDIFITLTGGKLN
                     A"
     gene            51575..52288
                     /locus_tag="VUW50_000765"
     CDS             51575..52288
                     /locus_tag="VUW50_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7982706.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNETLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(52610..52702)
                     /locus_tag="VUW50_000766"
                     /pseudo
     CDS             complement(52610..52702)
                     /locus_tag="VUW50_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            53023..53724
                     /gene="pyrF"
                     /locus_tag="VUW50_000767"
     CDS             53023..53724
                     /gene="pyrF"
                     /locus_tag="VUW50_000767"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET7982707.1"
                     /translation="MREHRPVIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGAAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            53758..54390
                     /locus_tag="VUW50_000768"
     CDS             53758..54390
                     /locus_tag="VUW50_000768"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET7982708.1"
                     /translation="MTLAKYIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVDAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
BASE COUNT        16918 a         9757 c        12025 g        16043 t
ORIGIN      
        1 catacatacg gcaaggcgac actgacgaag tttgaattgg attttcgcag agtgtaaatt
       61 gttgaaacta ggttgtcata ccatgtatac cacttgggct ttggcagtag ctaactgcac
      121 taaatataat ataaggaggg ttttatgggg aaaggccatt ggaatcggaa aagagtttat
      181 agcattcgta agtttgctgt gggagcttgc tcagtaatga ttgggacttg tgcagttcta
      241 ttaggaggaa atatagctgg agaatctgta gtttatgcgg atgaaacact tattactcat
      301 actactgaga aacctaaaga ggaaaaaatg atagtagaag aaaaggctga taaagctttg
      361 gaaactaaaa atgtagttga aaggacagaa caaagtgaac ctagttcaac tgaggctatt
      421 gcatctgaga agaaagaaga tgaagccgta actccaaaag aggaaaaagt gtctgctaaa
      481 ccggaagaaa aagctccaag gatagaatca caagcttcaa gtcaagaaaa accgctcaag
      541 gaagatgcta aagctgtaac aaatgaagaa gtgaatcaaa tgattgaaaa caggaaagtg
      601 gattttaatc aaaattggta ctttaaactc aatgcaaatt ctaaggaagc cattaaacct
      661 gatgcagacg tatctacgtg gaaaaaatta gatttaccgt atgactggag tatctttaac
      721 gatttcgatc atgaatctcc tgcacaaaat gaaggtggac agctcaacgg tggggaagct
      781 tggtatcgca agactttcaa actagatgaa aaagacctca agaaaaatgt tcgccttact
      841 tttgatggcg tctacatgga ttctcaagtt tatgtcaatg gtcagttagt ggggcattat
      901 ccaaatggtt ataaccagtt ctcatacgat atcaccaaat acctttacaa agatggtcgt
      961 gagaatgtga ttgctgtcca tgcagtcaac aaacagccaa gtagccgttg gtattcagga
     1021 agtggtatct atcgtgatgt gactttacaa gtgacagata aggtgcatgt tgagaaaaat
     1081 gggacaacta ttttaacacc aaaacttgaa gaacaacaac atggcaaggt tgaaactcat
     1141 gtgaccagca aaatcgtcaa tacggacgac aaagaccatg aacttgtagc cgaatatcaa
     1201 atcgttgaac gaggtggtca tgctgtaaca ggcttagttc gtacagcgag tcgtacctta
     1261 aaagcacatg aatcaacaag cctagatgcg attttagaag ttgaaagacc aaaactctgg
     1321 accgttttaa atgacaaacc tgccttgtac gaattgatta cgcgtgttta ccgtgacggt
     1381 caattggttg atgctaagaa ggatttgttt ggttaccgtt actatcactg gactccaaat
     1441 gaaggtttct ctttgaatgg tgaacgtatt aaattccatg gagtatcctt gcaccacgac
     1501 catggggcgc ttggagcaga agaaaactat aaagcagaat atcgccgtct caaacaaatg
     1561 aaggagatgg gagttaactc catccgtaca acccacaacc ctgctagtga gcaaaccttg
     1621 caaatcgcag cagaactagg tttactcgtt caggaagagg cctttgatac ttggtatggt
     1681 ggcaagaaac cttatgacta tggacgtttc tttgaaaaag atgccactca cccagaagct
     1741 cgaaaaggtg aaaaatggtc tgattttgac ctacgtacca tggtcgaaag aggcaaaaac
     1801 aaccctgcta tcttcatgtg gtcaattggt aatgaaatag gtgaagctaa tggtgatgcc
     1861 cactctttag caactgttaa acgtttggtc aaggttatca aggatgttga taagactcgc
     1921 tatgttacca tgggagcaga taaattccgt ttcggtaatg gtagcggagg gcatgagaaa
     1981 attgctgatg aactcgatgc tgttggattt aactattctg aagataatta caaagccctt
     2041 agagctaagc atccaaaatg gttgatttac ggttcagaaa catcatcagc aacccgtaca
     2101 cgaggaagtt actatcgccc tgaacgtgaa ttgaaacata gcaatggacc tgagcgtaat
     2161 tatgaacagt cagattatgg aaatgatcgt gtgggttggg ggaaaacagc aaccgcttca
     2221 tggacttttg accgtgacaa cgctggctat gctggacagt ttatctggac aggtacggac
     2281 tatattggtg aacctacacc atggcacaac caaaatcaaa ctcccgttaa gagctcttac
     2341 tttggtatcg tagatacagc cggcattcca aaacatgact tctatctcta ccaaagccaa
     2401 tgggtttctg ttaagaagaa accgatggta caccttcttc ctcactggaa ctgggaaaac
     2461 aaagaattag catccaaagt agctgactca gaaggtaaga ttccagttcg tgcttattcg
     2521 aatgcttcta gtgtagaatt gttcttgaat ggaaaatctc ttggtcttaa gactttcaat
     2581 aaaaaacaaa ccagcgatgg gcggacttac caagaaggtg caaatgctaa tgaactttat
     2641 cttgaatgga aagttgccta tcaaccaggt accttggaag caattgctcg tgatgaatct
     2701 ggcaaggaaa ttgctcgaga taagattacg actgctggta agccagcggc agttcgtctt
     2761 attaaggaag atcatgcgat tgcagcagat ggaaaagact tgacttacat ctactatgaa
     2821 attgttgaca gccaggggaa tgtggttcca actgctaata atctggttcg cttccaattg
     2881 catggtcaag gtcaactggt cggtgtagat aacggagaac aagccagccg tgaacgctat
     2941 aaggcgcaag cagatggttc ttggattcgt aaagcattta atggtaaagg tgttgccatt
     3001 gtcaaatcaa ctgaacaagc agggaaattc acccttactg cccactctga tctcttgaaa
     3061 tcgaaccaag tcactgtctt tactggtaag aaagaaggac aagagaagac tgttttgggg
     3121 acagaagtgc caaaagtaca gaccattatt ggagaggcac ctgaaatgcc taccactgtt
     3181 ccgtttgtat acagtgatgg tagccgtgca gaacgtcctg taacctggtc ttcagtagat
     3241 gtgagcaagc ctggtattgt aacggtgaaa ggtatggctg acggacgaga agtagaagct
     3301 cgtgtagaag tgattgctct taaatcagag ctaccagttg tgaaacgtat tgctccaaat
     3361 actgacttga attctgtaga caaatctgtt tcctatgttt tgactgatgg aagtgtacaa
     3421 gagtatgaag tggacaagtg ggagattgcc gaagaagata aagctaagtt agcaattcca
     3481 ggttctcgta ttcaagcgac cggttattta gaaggtcaac caattcatgc aacccttgtg
     3541 gtagaagaag gcaatcctgc agcacctgta gtgccaactg ttactgttgg aggtgaagca
     3601 gtaacaggtc ttactagtcg acaaccaatg caatatcgta ctctatctta tggtgcccaa
     3661 ttgccagaag tcacagcaag tgctaaaaat gcagctgtta cagttcttca agcaagcgca
     3721 gcaaacggca tgcgtgcgag catctttatt cagcctaaag atggtggccc tcttcaaacc
     3781 tatgcaattc aattccttga agaagcgcca aaaattgctc acttgagctt gcaagtggaa
     3841 aaagctgaca gtctcaaaga agaccaaact gtcaaattgt cggttcgagc tcactatcaa
     3901 gatggaacgc aagctgtatt accagctgat aaagtaacct tctctacaag tggtgaaggg
     3961 gaagtcgcaa ttcgtaaagg aatgcttgag ttgcataagc caggagcagt cactctgaac
     4021 gctgaatatg agggagctaa aggccaagtt gaactcacta tccaagccaa tactgagaag
     4081 aagattgcgc aatccatccg tcctgtaaat gtagtgacag atttgcatca ggaaccaagt
     4141 cttccagcaa cagtaacagt tgagtatgac aaaggtttcc ctaaaactca taaagtcact
     4201 tggcaagcta ttccgaaaga aaaactagac tcctatcaaa catttgaagt actaggtaaa
     4261 gttgaaggaa ttgaccttga agcgcgtgca aaagtctctg tagaaggtat cgtttcagtt
     4321 gaagaagtca gtgtgacaac tccaatcgca gaagcaccac aattaccaga aagtgttcgg
     4381 acatatgatt caaatggtca cgtttcatca gctaaggttg catgggatgc gattcgtcca
     4441 gagcaatacg ctaaggaagg tgtctttaca gttaatggtc gcttagaagg tactcaatta
     4501 acaactaaac ttcatgttcg cgtatctgct caaactgagc aaggtgcaaa catttctgac
     4561 caatggaccg gttcagaatt gccacttgcc tttgcttcag actcaaatcc aagcgaccca
     4621 gtttcaaatg ttaatgacaa gctcatttcc tacaataacc aaccagccaa tcgttggaca
     4681 aactggaatc gtactaatcc agaagcttca gtcggtgtcc tattcggaga ttcaggtatt
     4741 ttgagcaaac gctccgttga taatctaagt gtcggattcc acgaagacca tggagttggt
     4801 gcaccgaagt cttatgtgat tgagtattat gttggtaaga ctgtcccaac agctcctaaa
     4861 aaccctagtt ttgttggtaa tgaggaccat gtctttaatg attctgccaa ctggaaacca
     4921 gttactaatc taaaagcccc tgctcaactc aaggctggag aaatgaacca ctttagcttt
     4981 gataaagttg aaacctatgc tgttcgtatt cgcatggtta aagcagataa caagcgtgga
     5041 acgtctatca cagaggtaca aatctttgcg aaacaagttg cggcagccaa gcaaggacaa
     5101 acaagaatcc aagttgacgg caaagactta gcaaacttca accctgattt gacagactac
     5161 taccttgagt ctgtagatgg aaaagttccg gcagtcacag caagtgttag caacaatggt
     5221 ctcgctaccg tcgttccaag cgttcgtgaa ggtgagccag ttcgtgtcat cgcgaaagct
     5281 gaaaatggcg acatcttagg agaataccgt ctgcacttca ctaaggataa gagcttactt
     5341 tctcataaac cagttgctgc ggttaaacaa gctcgcttgc tacaagtagg tcaagcactt
     5401 gaattgccga ctaaggttcc agtttacttc acaggtaaag acggctacga aacaaaagac
     5461 ctgacagttg aatgggaaga agttccagcg gaaaatctga caaaagcagg tcaatttact
     5521 gttcgaggcc gtgtccttgg tagtaacctt gttgctgagg tcactgtacg agtgacagac
     5581 aaacttggtg agactctttc agataaccct aactatgatg aaaacagtaa ccaggccttt
     5641 gcttcagcaa ccaatgatat tgacaaaaac tctcatgacc gcgttgacta tctcaatgac
     5701 ggagattatt cagaaaatcg tcgttggaca aactggtcac caacaccatc ttctaatcca
     5761 gaagtatcag cgggtgtgat tttccgtgaa aatggtaaga ttgtagaacg gactgttgca
     5821 caaggaaaag ttcagttctt tgcagatagt ggtacggatg caccatctaa actcgtttta
     5881 gaacgctatg tcggtccaga gtttgaagtg ccaacctact attcaaacta ccaagcctac
     5941 gacgcagacc atccattcaa caatccagaa aattgggaag ctgttcctta tcgtgcggat
     6001 aaagacattg cagctggtga tgaaatcaac gtaacattta aagctatcaa agccaaagct
     6061 atgagatggc gtatggagcg taaagcagat aagagcggtg ttgcgatgat tgagatgacc
     6121 ttccttgcac caagtgaatt gcctcaagaa agcactcaat caaagattct tgtagatgga
     6181 aaagaacttg ctgatttcgc tgaaaatcgt caagactatc aaattaccta taaaggtcaa
     6241 cggccaaaag tctcagttga agaaaacaat caagtagctt caactgtggt agatagtgga
     6301 gaagatagct ttccagtact tgttcgcctc gtttcagaaa gtggaaaaca agtcaaggaa
     6361 taccgtatcc acttgactaa ggaaaaacca gtttctgaga agacagttgc tgctgtacaa
     6421 gaagatcttc caaaaatcga atttgttgaa aaagatttga catacaagac agttgagaaa
     6481 aaagattcaa cactgtatct aggtgaaact cgtgtagaac aagaaggaaa agttggaaaa
     6541 gaacgtatct ttacagcgat taatcctgat ggaagtaagg aagaaaaact ccgtgaagtg
     6601 gtagaagttc cgacagaccg catcgtcttg gttggaacca aaccagtagc tcaagaagct
     6661 aaaaaaccac aagtgtcaga aaaagcagat acaaaaccaa ttgattcaag tgaagctagt
     6721 caaactaata aagcccagtt accaaataca ggtagtgcgg caagccaagc agcagtagca
     6781 gcaggtttag ctcttctagg tttgagtgca ggattagtag ttactaaagg taaaaaagaa
     6841 gactagaaat tttgatacct tctttatcat taaagaccaa agaaaaactc ttgtcctaaa
     6901 tagaagataa agagtctgga acaaaatagt tctcaaccac aaaaaagcta gatatttcca
     6961 attgcggaac tctagctttt taattttgag tcgttctctt attgtatagt agattgaaac
     7021 tagagtagta cacttctact tctaaaacat tgttagaaat cgatttgact gtccttatcg
     7081 atttgtcctg ttcttatttc atttcactat gttttaggag gttattggcc ataagtacca
     7141 atcccatgtc aattctcact tgacgcttgc ccctcagatt acatctcttg tgacccaaac
     7201 aagcctttat ctgcccaaag acaggtttca catcaatctt gcgttgagcg aaaatctgtc
     7261 taccttgggg agataaaagc gcctgacatt ctttagcttt caagttttga tagcgttcgt
     7321 tcatatacag tcctttttga ggggctgatt caggttcatc agcgtagtaa accttgattt
     7381 cctgttgaaa gtctgtctgt gttttctggt gcttgatatg gtgaaaacga tagcaccagc
     7441 catcaggatg tatgtagcta tcctccttgt cattatagtg ccaattcgct cagtttttag
     7501 ctgactcttt ataccctctc ttttgtttct tatcaaacat ggcatattta atcagaaggg
     7561 aagtgatttc ttcttgaaaa tagacttgta tctgttcttg aagtttggcg gagaatttct
     7621 ctgtcgtttt cttccacaca aaactatact tgttggcatt ggcttcaatc ttagttccgt
     7681 caataaacaa acaatctaag gtcactaact cttccatttt tcaatttttc gaccagagaa
     7741 aatcccttgt gaataggcaa atagaagagt agatacaagc attttagggt gataagacgg
     7801 gcgatcaaag gcatgatagg aggtgtagaa gtgacgttcc tccaaggtat tcaccacttt
     7861 ttcaatagta aagacgagat ggtcttgtgg taagaaggaa ctgatttcta gtggtaaagt
     7921 tgtttgattt gtgttatagt gaatatgcat gggatagcct ttttaaatgg tttattaagc
     7981 aattctattt taccaagact atcgtaacca tgcaaaaaaa gcaacggcaa atccgttgct
     8041 ttttttggag ataagagact attgtcccag gctcttttct ttgtataaaa ttgtatagtg
     8101 ttattgtcta tactattttg tagagaaagc aaaaatccct agaaaatgct tagggattcg
     8161 actatttcat taagaacaat tcaacaatca tttctatata catgataagg gttaataaga
     8221 atcctgcacg ccagaagaat ttgatgaatt taggatagta aaagctgcgt ttttttaaaa
     8281 ggaagaaaaa gacgagaata atggctagga acgagagggc tagccccagt ctagggagga
     8341 aattatgggt aaaggtttta gctgtaatca agtaatactc aaataccaag actggaaaag
     8401 ccaaatccgc aaagtttcgt cctagttttt ttaatctaaa aagtttggtt aggataatgc
     8461 agacgactaa ggtcagtatc aataataaaa tagatgctaa tttcattaaa atcataccca
     8521 tattgtatca taaaaaaagg ctaatggaaa cgagaaacag aggaatttat aggaaagtca
     8581 ggcttttgag attgtgggtg atttttgtta taatgaaagt tataaaatct tataatacct
     8641 tgaatgatta ttatcgaaaa ctgtttggag aaaagacttt taaagtccct attgatgcgg
     8701 gatttgactg tcccaatcgt gatggaactg tggctcatgg aggctgtact ttttgtacgg
     8761 tttcaggttc tggagatgct attgtagccc cagatgcgcc tatccgtgag caattttata
     8821 aggaaattga ctttatgcac cgtaagtggc cggatgttca gaagtatctg gtttattttc
     8881 aaaattttac caacacccat gaaaaggtgg aagttattcg agagcgttat gaacaggcta
     8941 tcaatgagcc aggtgtggta ggtatcaata ttggaacgcg cccagactgt ttaccagacg
     9001 aaaccatcga atatttggct gagttatcgg agtgcatgca tgtgatggtt aaattgggct
     9061 tgtagactac ttatgaagca acttctgacc tgattaaccg tgcgcattct tatgaattgt
     9121 aggtggaaac ggtcaagcgt ttgagaaagt atcccaagat tgagattgtt tcccatttga
     9181 tcaatggttt gcctggtgaa acccatgaga tgatggttga aaatgtccgc cgttgtgtca
     9241 cgaataacga tattcaaggg attaaactgc atttgctcca tcttatgaca aatactcgta
     9301 tgcaacgaga ttaccatgag ggacgtttac aattgatgag tcaggacgaa tatgtcaggg
     9361 tcatctgtga ccaactggaa attattccca aacatatcgt catccatcga attacaggag
     9421 atgcgcctag ggatatgctg attggtccta tgtggagcct caaaaaatgg gaagtcctca
     9481 acagcattga gatggagatg cgacgtcgtg gaagtgttca aggatgcaag gctgtaaaac
     9541 aggagtttga aaatgaaaag accacttgag atggcacatg attttttggc tgaggtcgtg
     9601 acaaaagagg atgtcgtagt ggatgcgact atgggaaatg gtcatgacac gcttttttta
     9661 gccaagctag ccaagcaagt ctatgccttt gatattcaga agcaagcctt ggaaaagacc
     9721 caagagcgtt tgcatcaggc tggcttgaca aatgcccagt taatcttgca aggccatgag
     9781 acactggacc agtttgtgac aaaagctaag gcagggattt ttaatctggg ctatttgccg
     9841 tcagctgata agtctgtcat cacccgaccg cagacaacga ttgaggcatt agaaaagcta
     9901 tgtggcttac ttgtcaaagg tggacgaatt gctattatga tttactatgg tcatgaagga
     9961 ggcgacctcg agagatatgc tgtcttggat tttgtgatcc agttgaacca acaagagtac
    10021 acagctgcca tttaccgaac tttaaaccaa gtcaacaacc cgccgttttt agtgatgatt
    10081 gaaaaattag agagatacag acatggataa acaataccta cacgaaaagc tggatgccat
    10141 gcgccagaat tttgttgaat caactcacca cgaacgagcg gtgggcgtgc tagaccaagc
    10201 gcatatgagc aaaaaaatgc ttaaaatcaa gaaaaaatta gttgctcttg aaatggaacg
    10261 gtgccagaga aaaattgagc acaaagactg ttccaagatt gaccaaaaaa tcaaagagca
    10321 gaaggagata tttgaatcct gttgtaaaaa agattaagga ggacgtgcgt ggaattactg
    10381 atttacctca tcctattttt actggttttg attgtctcaa gtacaaccaa taagctcctg
    10441 ccctttttgc ctctcccttt ggtgcaaatt cttttgggaa ttgtgattgg tctcttttta
    10501 cccaataccg actttcacct caatacggag ttgtttttgg ccctggttat cggacccttg
    10561 cttttccgag aggctgaaga agcagatgtt acggctattt taaaacactg gcgaatcatt
    10621 gtttatctca tatttccagt gatttttatc tcgaccctga gtttgggtgg cttggcccat
    10681 cttctttggt tcagccttcc cttggcagct tgcttggctg ttggggcagc ccttggtcct
    10741 acggacttgg tggcctttgc ctctctttcg gagcgtttta gctttcctaa gcgcgtgtcc
    10801 aatattctta agggcgaagg actcttgaat gatgcttctg gtttggtggc ttttcaggta
    10861 gctttgacag cttggacaac tggagctttt tctctggggc aagctagcag ttcgctcatc
    10921 ttttcaatcc taggcggttt tttaattgga tttttaacag ccatgaccaa ccgcttcctc
    10981 cataccttct tgctaagtgt gcgcgcaacg gatattgcca gtgaactttt attagaattg
    11041 agtttgcctc tagtgacctt ctttctggca gaagaagtcc atgtttcagg gattattgcc
    11101 gtcgtagttg ctggaatttt aaaggcaagt cgcttcaaga aaatcacgct cctcgaagcc
    11161 caagtggata cggtgaccga gacggtctgg catacagtga cctttatgct caacggttct
    11221 gtctttgtga ttttagggat ggagttggaa atgatagcag aacctatctt gaccaatcca
    11281 atctataatc ctctactttt attgctatct ctcatcgccc ttacctttgt cctctttgtc
    11341 attcgtttta ttatgatcta tggctattat gcctatagaa cccgacgcct caagaaaaag
    11401 ctaaataagt atatgaagga catgtttctc ttgacctttt caggtgttaa gggaacggtg
    11461 tcgattgcta cgattctctt gataccaagt aatctagaac aggagtatcc tctcttgctt
    11521 ttccttgttg caggtgtgac gcttgtcagc tttttaacag gtctcttggt tttgcctcat
    11581 ctttctgatg aagaggaaga aagcaaggat tatctcatgc atatcgccat tttgaatgaa
    11641 gtaacgctag agttggaaaa agagttggaa gacaccagaa ataaacttcc cctctatgcg
    11701 gctattgaca attatcatgg acgtattgaa aatctcattt taagccaaga aaaccaggat
    11761 gatcaagaag actgggctgc tttgaaactc ttgattctta gtattgaaag tgatggtttg
    11821 gaacaggcct atgaagaggg gaacattagc aatcgtgctt accgagttta ccaacgttat
    11881 ctgaaaaata tagaacaagg aatcaatcgt aaacttgcct caagactgac ctattatttt
    11941 cttgtttcct tgaggatttt acgttttctc cttcatgaag tttttactct tggaaagacc
    12001 ttccgtagct ggaaggacaa ggagcaaagc cgtctccgtg ctcttgatta tgaccaaatt
    12061 gcagagctct atcttggcaa tacggagatg attattgaaa gtttggaaaa cctgaaggga
    12121 gtctacagac gctctttgat tagttttatg caggagtctc gtcttcgaga aacaactatt
    12181 atcagcagtg gtgcctttgt cgaacgggtt atcaatcgtg tcaaacccaa caatatcaat
    12241 gaaatgctga gaggctatta tctggagcgc aagttgattt tcgaatacga agaaaaacga
    12301 ttaattacaa ctaagtatgc caagaaatta cgacaaaatg taaataactt agagaactat
    12361 tccttgaagg aagctgccaa taccctgccg tatgatatgg tggaattggt aagaagaaat
    12421 tagttaatac tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttac cgtatgtatg
    12481 tgactgactt cgtcagtctt atctacaacc tcaaaacagt gttttgagca gcttgcggct
    12541 agctttctag tttgctcttt gattttcatt gagtataaga ttataaatga aggagtatga
    12601 catgaaaaag tggtggaaag agctgataga caagccttta ttaaaagctt ttttgcatta
    12661 ttatcaagca tcagatagtg agttgaccag tgtcgcagta gcctactatt ggttgatttc
    12721 gattttcccc ctgcttttgg tggtggtcaa tatcctcccc tattttcaga ttcctgtggg
    12781 agaatttctg ggctttatga aagacgtcct gcccccaagc ctctatgaag gtgtggaaaa
    12841 aatagccaga gaagtcttga cgcaaccttc aacaggttta ttgagttttt ctgttttatc
    12901 ggcgttatgg agtttttcaa aatctataaa tttcttgcaa aaagctttta acaaggctta
    12961 tggcgtcgaa aagagtcgtg gacttatttc ccatcagatg ctaagtctcc tagtcagttt
    13021 tggcttgcag ttcctctttg cgtttgcctt gtttttgatc ttatttggtc agatgatttt
    13081 ggctttactt gctcattatt ggacaaaaga tggcattatc tatcaggctt tacaagggtt
    13141 ggcaggtcct ctgatttacg ccctcctctt tgctatcttg gtcatgcttt attattttct
    13201 tcccaatcta tctaacagga aaattagcta taccctacca ggcagtgccg ttgttctctt
    13261 ggtgatttta ggtgtactaa ctctgttctc tagttatctc aattactatg tccatcattt
    13321 ggtggatgtc cgaattttag gttccgttct tcttgttgtt atgatgtttt ggtttatctt
    13381 gattgctaaa attgttatcc taggcgcggt tatcaatgcc agtatgcaga gtttgaaaga
    13441 tccggtcttt aaaaaagact aactatttat ccattataaa agcgcatagt atcaagactt
    13501 tatagaacct tgatactatg cgcttttgtg ctttgacagt tttttgtata gtttttgtcc
    13561 aagatttcta aattatctga ttaaattcct attttcccct atcattgtcc ctgttttttc
    13621 gaaaatggaa ggagtataat aaatctatca ataaaatgat aggaggaact ttatgggtca
    13681 tattttcttt tttctaagtg tctttttggc agggattcta tccttctttt ctccttgtat
    13741 cttacctttg ttaccggtct atacaggagt gttactagat gataaggatg gtgctcaggc
    13801 ttctagcggc aaattttcaa tctcagttac tagtttatta cgaactctgg cctttatagc
    13861 aggaatttcc tttatattta ttttgttggg ctatggagct ggttttttag gcgatttgct
    13921 ttatgcttct tggttccaat atcttactgg ggcaattatt atctttcttg gtttgcacca
    13981 aatggagatt ctacacttta aggggcttta taaggaaaag aggctacaac tgcaaggaca
    14041 ggggcaaaat ggtaagggct atagtcaggc atttttattg ggcttgacct ttagttttgc
    14101 ttggacgcct tgcgtggggc cggttctggg gtctgttttg gccttggcgg cttcaggtgg
    14161 ttcaggagct tggcagggag ctggtctcat gttggtgtat acgctgggct tggcgctacc
    14221 attcttgctt ctagctctga cctctagtta tgttttgaaa catttccgaa aacttcatcc
    14281 ctatctcgga atcctcaaaa aagtgggtgg ttttctcatt attgtgatgg gactcttggt
    14341 tctgtttgga aatgcttcaa ttttaagtca attatttgaa taaaatggaa aggaatatca
    14401 atatgaaaaa atggcaaaca tgtgttcttg gagcaggttc gctcctttgt ttgacggctt
    14461 gttcaggcaa gtccgtgact agtgaacacc aaacgaaaga tgaaatgaag acggagcaga
    14521 cagctagtaa aacaagcgca gctaaaggga aagaggtggc tgattttgaa ttgatgggag
    14581 tagatggcaa gacctaccgt ttatctgatt acaagggcaa gaaagtctat ctcaaattct
    14641 gggcttcttg gtgttccatc tgtctggcta gtcttccaga tacggatgag attgctaaag
    14701 aagctggtga tgactatgtg gtcttgacag tagtgtcacc aggacataag ggagagcaat
    14761 ctgaagcgga ctttaagaat tggtataagg gattggatta taaaaatctc ccagtcctag
    14821 ttgacccatc aggcaaactt ttggaaactt atggtgtccg ttcttaccca acccaagcct
    14881 ttatagacaa agaaggcaag ctggtcaaaa cacatccagg attcatggaa aaagatgcaa
    14941 ttttgcaaac tttgaaggaa ttagcctagg aggcgtctta tgaatgataa gttaaaaatc
    15001 ttcttgttgc taggagtatt ttttctagcc ataaccggtt tctatgttct attgatacga
    15061 aatgcagggc agacagatgc ctcgcaaatt gaaaaggcgg cagttagcca aggaggaaaa
    15121 gcagtgaaaa aaacagaaat tagtaaagac gcagacttgc acgaaattta tctagctgga
    15181 ggttgtttct ggggagtgga ggaatatttc tcacgtgttc ccggggtgac ggatgccgtt
    15241 tcaggctatg caaatggtag aggagaaaca accaagtacg aattgattaa ccaaacaggt
    15301 catgcagaaa ccgtccatgt cacctatgat gccaagcaaa tttctctcaa ggaaatcctg
    15361 cttcactatt tccgcattat caatccaacc agcaaaaata aacaaggaaa tgatgtgggg
    15421 acccagtacc gtactggtgt ttattacaca gatgacaagg atttggaagt gattaaccaa
    15481 gtctttgatg aggtggctaa gaaatacgat caacctctag cagttgaaaa ggaaaacttg
    15541 aagaattttg tggtggctga ggattaccat caagactatc tcaagaaaaa tccaaatggc
    15601 tactgccata tcaatgttaa tcaggcggcc tatcctgtca ttgatgccag caaatatcca
    15661 aaaccaagtg atgaggaatt gaaaaagacc ctgtcacctg aggagtatgc agttacccag
    15721 gaaaatcaaa cagaacgagc tttctcaaac cgttactggg ataaatttga atccggtatc
    15781 tatgtggata tagcaactgg ggaacctctc ttttcatcaa aagacaaatt tgagtctggt
    15841 tgtggctggc ctagttttac ccaacccatc agtccagatg ttgtcaccta caaggaagat
    15901 aagtcctaca atatgacgcg tatggaagtg cggagccgag taggagattc tcaccttggg
    15961 catgtcttta cggatggtcc acaggacaag ggcggcttac gttactgtat caatagcctc
    16021 tctatccgct ttattcccaa agaccaaatg gaagaaaaag gctacgctta tttactagat
    16081 tatgttgatt aatactcttc gaaaatctct tcaaaccacg tcagcgtcgc cttaccgtat
    16141 gtagggttac tgacttcgtc agttttatct acaacctcaa agctatactt tgagctgact
    16201 tcgtcagttt tatttacaac ctcaaagcgg tactttgaga aacctgtggc tatcttccta
    16261 gtttactttt tgatttttat tgagtataag aggtctttcc taagcagtta gaggagaatt
    16321 ttgctatact gatactagta agtggtaaag gagcagagca tgacctacac aatcttaatc
    16381 gtagaagatg aatatctggt aagacaaggt ttgactaaac tggtcaatgt agcagcctac
    16441 gatatggaaa tcatcggtca ggctgaaaat ggaaggcagg cttgggaatt gatccaaaag
    16501 caggtgccag atatcatttt aaccgatatc aacatgcctc atctaaatgg catccagttg
    16561 gccagtctgg tacgagaaac ctatcctcag gttcatttgg tctttttaac aggttacgat
    16621 gattttgatt atgccttgtc tgctgtcaaa ctaggtgtgg acgactacct gctcaaaccc
    16681 ttttctcgtc aggatattga ggaaatgttg gggaaaatca aacaaaaact agacaaggaa
    16741 gagaaagaag agcagttaca agatttattg actaataggt ttgaaggaaa catggcccag
    16801 aaaatccagt ctcatctggc tgatagccaa tttagtttaa agtctttagc cagtgactta
    16861 ggttttagtc cgacctatct gagttccttg attaagaaag agttgggctt gccttttcag
    16921 gattatctgg tgagagaacg tgttaaacaa gccaagctct tgcttttaac tacagatctg
    16981 aagatttatg agatcgcaga gaaggttggt tttgaagata tgaactattt tacccaacgt
    17041 tttaagcaga ttgcaggtgt gacacctcgt cagtttaaga agggagaaga ccgatgaagc
    17101 gttcttctct tttagttaga atggttattt ccatctttct ggtctttctc attctcctag
    17161 ctctggttgg aactttctac tatcaatcaa gttcttcagc cattgaggcc accattgagg
    17221 gcaacagcca aacgaccatc agccagacta gccactttat tcagtcttat atcaaaaaac
    17281 tagaaaccac ctcgactggt ttgacccagc agacggatgt tctggcctat gctgagaatc
    17341 ccagtcaaga caaggtcgag ggaatccgag atttgttttt gaccatcttg aagtcagata
    17401 aggacttgaa aactgttgtg ctggtgacca aatctggtca ggtcatttct acagatgaca
    17461 gtgtgcagat gaaaacttcc tctgatatga tggctgagga ttggtaccaa aaggccattc
    17521 atcagggagc tatgcctgtt ttgactccag ctcgtaaatc agatagtcag tgggtcattt
    17581 ctgtcactca agaacttgtt gatgcaaagg gagccaatct tggtgtgctt cgtttggata
    17641 tttcttatga aactctggaa gcctatctca atcaactcca gttggggcag cagggctttg
    17701 ccttcattat caatgaaaac catgaatttg tctaccatcc tcaacacaca gtttatagtt
    17761 cgtctagcaa aatggaggct atgaaaccct acatcgatac aggtcagggt tatactcctg
    17821 gtcacaaatc ctacgtcagt caagagaaga ttgcaggaac tgattggacg gtgcttggcg
    17881 tgtcatcatt ggaaaagtta gaccaggttc ggagtcagct cttgtggacc ttgcttgggg
    17941 ccagtgtcac atctcttctt gtctgtctct gcttagtgtg gttcagtctt aaacgctgga
    18001 ttgctccttt gaaggatttg agagaaacca tgttggaaat tgcttctggt gctcaaaatc
    18061 ttcgtgccaa ggaagttggt gcctatgaac tgagagaagt aactcgccaa tttaatgcta
    18121 tgttggatca gattgatcag ttgatggtag ctattcgtag ccaggaagaa acgacccgtc
    18181 agtaccaact tcaagccctt tcgagccaga ttaatccaca tttcctctat aacactttgg
    18241 acaccatcat ctggatggct gaatttcatg atagtcagcg agtggtgcag gtgaccaagt
    18301 ccttggcaac ctatttccgc ttggcgctca atcaaggcaa ggacttgatt tgtctctctg
    18361 acgaaatcaa tcatgtccgc cagtatctct ttatccagaa acaacgctat ggagataagc
    18421 tggaatacga aattaatgaa aatgttgcct ttgataattt agtcttaccc aagctggtcc
    18481 tacaacccct tgtagaaaat gctctttacc atggcattaa ggaaaaggaa ggtcagggcc
    18541 atattaaact ttctgtccag aaacaggatt cgggattggt catccgtatt gaggatgatg
    18601 gcgttggctt ccaagatgct ggtgatagta gtcaaagtca actcaaacgt gggggagttg
    18661 gtcttcaaaa tgtcgatcaa cggctcaaac ttcattttgg agccaattac catatgaaga
    18721 ttgattctag accccaaaaa gggacgaaag ttgaaatata tataaataga atagaaacta
    18781 gctaactccc agtcaattct gggagtttta tgcgtaattg ggcaaacttt ctaggttgtc
    18841 tatcttgcta aaaacaagaa aaaacggtat gatagaagag tgacaacaga ggtaacagag
    18901 atgaacgaga cacagattca aagagaaaca cgtcaggtag tggaagatgt tttagaaaag
    18961 accaatttga agcagggggc cctttttgtt ttaggtctat cttctagtga ggtgctaggt
    19021 ggtcatattg ggaaagaatc cagtcaagag attggtgagc tgatagtgga gactatcttg
    19081 gatattctag gtagtagagg tatccattta gcagttcaag gttgtgagca tgtcaatcgg
    19141 gctctcgttg ttgaacgtca ggtggcagag cagtttggtc tggaaattgt cagtgtccat
    19201 cctactcttc atgcaggagg ttcggggcag ttggcagcct ttaagtttat gcaggatcca
    19261 gttgaggttg aatttatcaa ggctcatgct ggattggata tcggagacac tgcaattggc
    19321 atgcatgtca agcatgttca ggttccgatt cgccctattt tgagagagat tggtcatgcc
    19381 catgtaacgg cactggctag tcgtccaaaa ttaatcggag gtgcgcgtgc gcactatccg
    19441 caagatgcta ttaggaagtt ttgagaatga ataaaggaga taaaagtgtt taaaaaagac
    19501 cgtttttcaa ttcgtaagat taagggagtt gtaggctctg tatttcttgg aagccttttg
    19561 atggctcctt ctgtagtgga cgcagccgcc tatcactatg taaataaaga gattatttca
    19621 caagaagcta aagatttaat tcagacagga aagcctgaca ggaatgaagt tgtatatggt
    19681 ttggtgtatc aaaaagatca gttgcctcaa acagggacag aagcatctgt tttgacagct
    19741 tttggtttgc tgactgttgg gagcttgctt ttaatctaca agagaaagaa aattgctagc
    19801 gtctttctag ttggagctat gggattggta gttcttccta gtgcaggggc tgtagaccca
    19861 gttgcgaccc tagcgctggc tagtcgagag ggtgttgttg aaatggaggg ctatcgctat
    19921 gttggttatc tatcaggtga catcctcaaa acgcttggct tggacactgt tttagaagaa
    19981 acctcagcaa aacctggaga ggtgactgtg gtcgaagttg agactcctca atcaataaca
    20041 aatcaggagc aagctaggac agaaaaccaa gtagtagaga cagaggaagc tccaaaggaa
    20101 gaaccaaaat cggaggtaaa acctactgac gacacccttc ctaaagtaga agaggggaaa
    20161 gaagattcag cagaaccagc tccagttgaa gaagtaggtg gagaagttga gtcaaaatca
    20221 gaggaaaaag tagcagttaa gccagaaagt caaccatcag acaaaccagc tgaggaatca
    20281 aaagttgaac aagcaggtga accagtcgcg ccaagagaag acgaaaaggc accagtcgag
    20341 ccagaaaagc aaccagaagc tcctgaagaa gagaaggctg tagaggaaac accgaaacaa
    20401 gaagatacac agccagaagt agtagaaaca aaagatgagg ctgctaatca acctgttgaa
    20461 gaaccaaaag ttgaaacgcc tgctgtagaa aaacaaacgg aaccaacaga ggaaccaaaa
    20521 gttgaacaag taggtgaacc agtcgcgcca agagaagacg aaaaggcacc agtctctcca
    20581 gaaaagcaac cagaagctcc tgaagaagag aagactgcgg aagaaacacc gaaacaagaa
    20641 gataaaataa agggtattgg tactaaagaa ccagttgata aaagtgagtt aaataatcaa
    20701 attgataaag ctagttcagt ttctcctact gattattcta cagcaagtta caatgctctt
    20761 ggacctgttt tagaaactgc aaaaggtgtc tatgcttcag agcctgtaaa acagcctgag
    20821 gtaaatagcg agacaaaagc tgaaaaagtt gcagctaata cagatgctaa acaaagtgaa
    20881 gtaaattctg agactgcaag tcttaaaaca gcaatttctg gattaaatac agataaagtt
    20941 gaactcgaaa atcaacttaa gattgcacag ggaaaaactg aaactgattt cagcatggaa
    21001 tcttggacgg ttttgagtac agctaaaaat aaagctcagg aagtaaaaga taacggaaca
    21061 gcgacccaag aacaaataaa cgaagcagag aaaagtttga aaacagccct tgctgactta
    21121 tctgttgaca aaacagcttt gggaagcgct atcgatacag ctaccaagaa aaataaagag
    21181 aactatacaa atcaaacatg ggcagagtta gaaacagttc ttacagcagc aaaatctgta
    21241 aacactaacg agtctaaaca aagtgaagtt aatgaagctg ttgaaaaatt aactgcaact
    21301 attgaaaaat tggttgaatt gtctgaaaag ccacgtttga ctctatctat tgaaaagaga
    21361 gacatagatc gaaaagttac ggtcacgtat actttggaaa atccagcaaa tactcaaata
    21421 aaatcaataa ctgcaactct gaaaaaagga gaggaagttg ttaaggattt tgttttaaca
    21481 gaagaaaatc ttaaaactaa tcatttaact gctttgtttg aaaagctaga ttactacaaa
    21541 gaatacactc tatctacgga tatggtatat aacagaggaa atgatgatga gacggagagt
    21601 atttcagaag aactaattca gctgaatctt aagaaactag aattgaaaga tatccagaca
    21661 gtctctctga tgaaatttga aaatggtcaa gaaagtcagg ttacacactt gtctgataaa
    21721 ccgaccgatt tgagtaaatt atacttaaaa gttacatcat caacgtctaa ggatgctgtg
    21781 ttagccgtta gctctataga agaagagatt gttgaaaata aaaaaatctt taaaattcat
    21841 gcagatacac cagaattagt agttcgtaag aaagatggca gtctttctaa gggatttgac
    21901 tattacatgg agagagttat ccctcatgat ggtgatattt attatgattt taaagacttg
    21961 atttcagcta tgacatctaa tcctacaggt acgtttattt tagggagaga tatttcctcg
    22021 cgtaatgtaa aaccggatgg aaatggaaaa tcttatatca aaggtgaatt taaaggaaaa
    22081 ctacttggta caaatgataa tgttagacac tcgatctttg acttagaata tccattgttt
    22141 gatactataa aatcaggagt cgtgaaagat attgatttca agcatgtcaa tatggtattc
    22201 ccagatagca atcaaggaga taacgtagca acaattgcaa gagtaatcaa agataagaca
    22261 aaaattgaga atgttaatgt tgaaggttat ctcgagggtc gagatcatgt agctggttta
    22321 gttaataatc ttgaaggtaa ttccgaaatt gaaaatatat cctttacagg aaagattaag
    22381 tctaaaggtg gtaattctat tacagcaggt attgcaggta gaaatatctt atctcgtgtt
    22441 aaacgtgcct atgtaaatgc aaatattgaa gtccttggtt caacgaattc gagtatgctt
    22501 gttgcagtaa atggtacaac attgaatgct agtggaggtt ggggaacctg gggaagactt
    22561 actgagtccg ttgctaaagg gacgttagaa attaaacgca gtggacaagc tggtggagtt
    22621 actgcaacag tctggcctta tggagcaatt gataaggttg tcagctatgc aaaagttacc
    22681 aaaggaaaag aattatttgg gtcagatggt gatttaaata ataattggtt tatgcaaaaa
    22741 attaataata tttttggtgt tcaaggtatc agctcaggtg actcagggaa tgattctaag
    22801 tttaagcgta tttccgagga agaagcaaag caaaaggttg cctcttataa tattacagct
    22861 cccaacttga tgagcgactc aagtcttttg gtagacagac tcaatgaaag ttggaaaaat
    22921 actgatcaat ttgaaagtat acaggattat caatcccaaa accaattaat ttaccagaac
    22981 ttgactaaat ttactcctta ttacaataaa gaatttattg ttcatgaagg aaatgctctc
    23041 actcctgaac aggaaatatt gaagactaag aaaatcaaat cgattgtagg gttgaaagga
    23101 acagagttcg ttgtagatgg cagtgatatt gatactatca tgcttcattt tgaggatggt
    23161 agtcagaagc gatataaggt aacctctact ggtaagtttt cgataactaa tttaccggaa
    23221 tatcaggttg aagatttaaa tgtggtgtat acatcagaac atatagtaca tccactagat
    23281 tcgtcactga taaataacct tgttgaagaa ttgaaaaaag tagaattgta tacagagtct
    23341 acttaccaag ttctaggtat tgataaagat aatgctaaca aattgaatag aactaagaga
    23401 ttattcttag atgagtcatt agatgctgtt aaaacacaat tgcctacctt tgtaaaaaca
    23461 atgtttgaaa atgagtggtt gcacatcaat ggagaatcta gtggagctgt cgctgcttta
    23521 cgacaaaaaa tcatggataa taagacggct atattgcttg ctttgactta tatcaatcgt
    23581 tattatgatg ttaaattctc agactataat attaaaaaac tgatgctctt taaaccaact
    23641 ttccatggtg aaaagattga tctattagat cgattaatcc gactaggatc atctggagaa
    23701 aatagattaa aaggttcaga aaatgcagaa acgttcaagc agttatttgc tagcgaaacg
    23761 aagcagaagg atttggtgac ttatttagac tacaatcgct ctttgttgac taattatcaa
    23821 acaacagggg aatggtttaa agaaacgact aaggattata ttcaatttga ggaacgtcca
    23881 tccttagtag aggagattaa agatgccaaa tatcgagttt acgataattt aaccgcaccg
    23941 tattatcaag gatatatcct acctctctta acgttgaaga atactcattt agcaatttta
    24001 tcgaactata gtactatgac atttgtcagc cgtgaaaaac gccctaattg gaaaaatgaa
    24061 gattttgata agtgggtgaa atatgtagca actgctcata gaaaccatgt tgatacttgg
    24121 tataagattt tacctgataa tatcaaaggt aaaatggtta aagagaatgt tactgctgtt
    24181 tgggaaggat tgagcatccc aggaagtgaa tgggtagacc agaatgctgt agatagaaaa
    24241 ggaagagatt acgccccagc tcgagaattc tttaatcttg taggtggtcc tatgggaggt
    24301 tggtatgctt accatggtta tggtgcgcat gctggaggaa gaaatcgggt taattatgag
    24361 gtgtttgatg tattaagtga gtatggtata tcggtcttta ctcatgaatt gactcacgtt
    24421 aatgatactt ggatttactt aggagggtat ggtagacgtg aaaatatggg acctgaagct
    24481 tatgctcagg gattattcca gtctcctgtt ccaggtcagc cgggttgggg ggcgcttggt
    24541 ctcaatatgg catttgaaag aaaaaatgat ggtgatctaa tttataatgc ctccccaact
    24601 caatttgaaa ataggaaaga gttagatagt tatatgaaaa attataacga tactctcatg
    24661 atggtggact acttggaagg agatgcagtt atctctaaag gtaaagaagc tattactaaa
    24721 tggtttaaaa aagttgaacc taaggttgtt agtcaaacag ctcagtatga tacggttcgt
    24781 cagttgactg ctgaagagaa agaaaaactt tctgtttcta gtgttgatga tttagttgat
    24841 caaggattaa tgtcagacag agctgtagga aataatacct ataatccagc tgattttgaa
    24901 acaagttata ttgcgataga ttatatgact ggtatttatg gtggaggaaa gaatagtgta
    24961 ggttcaccag gtgccttgat gtttaaacat aatactttcc gtatgtgggg ctactatggt
    25021 tttgaagaag gtgttctagg atatgcttct aataaattta agcaagcatc tagagatgaa
    25081 ggtcatgctg gacttagtga taactttatt atttctaaga tttccaaagg ggaattttta
    25141 actatggaag catttaaaaa gggatatttt aagaaagtag tagaagagtt aaaaactaaa
    25201 ggtattcgtc ctgttactat caatcaaaaa acatatagca cctttgaaga attgcaagaa
    25261 ggatttaagc aggctgttga aagagatttg aagaaaaatc agttagatga gagggaaaca
    25321 agaaacttca aattccaagt tttcagacaa cttcttcaac aaacagatag ctttaaaact
    25381 tctatcttta gataatcgaa atctctcatc tgctttgcgg atgagggttt tcttatttta
    25441 tgctatactt aaggtatgca tagaaaaaca gtgattgatt ttaggacttt gggggagaga
    25501 tataccttta cccagcctat taaagagttg aaaacgagaa atgtagaaga agtggcagat
    25561 ttgctggcac aagtggaaag ctaccaagag caaggttatt atgtggtggg gtatgtcagc
    25621 tacgaggctg cacctgcttt tgaggaaaaa ttagcagttc acaaggatcc cctactggga
    25681 gagtacctgc tttattttac tgttcatgat agcgtagaaa cttcccctat tcctctgact
    25741 tatgaggata ttgatttgcc ctcaaattgg caggaagtaa cgtctgcagc agactatgaa
    25801 aaggccattg cccagataca ccatcacttg aggcagggag acacctatca ggtcaactac
    25861 accgtccaac tcaagcaaaa gttaagtgcc aatccttttg ccatctacaa tcgtatggtg
    25921 gtagagcagg aggcgggcta caatgcctat gtggaacatg acgagatggc agtgatttcc
    25981 atgagcccag agctcttttt tgagcaaaat gatcgcgagt tgacaacacg accaatgaag
    26041 gggacgactc agcgtggggt aactgaccaa gaagatcttg aacaggccag ttggttggaa
    26101 caggatccca aaaatcgctc tgaaaatatg atgattgtgg acctcttgcg caatgatatg
    26161 aaccgtattt ctgaagttgg gagcgagcac gtggagcgtc tgtgccaggt agagcagtat
    26221 tcaactgttt ggcagatgac ttcgaccatc aagagtcagt tgcgagagga tgtggacctt
    26281 gttgaaatct tccgctcact ctttccttgt ggttccataa cgggtgcacc gaaaattgcg
    26341 acaatggaga ttatcaagaa cttggagcct caaccgcgtg gagtctactg tggaacgatt
    26401 ggtctcttgc ttccaaatgg acgacggatt tttaatgtgg ccattcgtac cattcaactt
    26461 cacaaaggtc aagccattta tggagttggc ggagggatta cttgggatag cacatgggaa
    26521 tctgaatacc gagaggttca tcaaaaggca gcagttcttt atcgtaaaca agctcgcttc
    26581 caactgatta cgacagggga aatcagccag aagaacctgc tgtttgaaga tcaacatctg
    26641 gaaagactga gaaaagctag tcgttatttt gcctttcctt ttgatgcaga agacttggga
    26701 cacaagattg aggaagagtg tcaggattgt gaagctaatc aagattaccg cttgcgaatc
    26761 agccttagca aatctggaga gatagaagtc aatcgtcaag tattaacccc tctcagtaca
    26821 agcttttgtc aggcccaagt ctgccttcag gaagctgctt tgaatcaatc ctttacctac
    26881 tttaaaacca ctcaccgacc gcatttgagc ctaggagaac aagagaagat ttaccacaat
    26941 aagtcaggag aactgcttga aacctctata ggaaatttgg ttctgaaaat cgctggaaaa
    27001 ctctacacac cgcctatccg acttggaatc ttgccaggaa tttaccgtca gcatttgcta
    27061 gaaacaggac aggtagaaga gaaagtcttg accttggcag atttaaccca agcagaagct
    27121 atttacggct gtaatgcagt gagaggcttg tatgaactaa gccttgagga gaactagatg
    27181 aaattgatat ttttgcatgg gttagggcag tcagcagagt cttggaaaga agttcggaat
    27241 ctactgacag attatccttc tgaggctatc gagttatttc cttctggagt tagtaactat
    27301 caacaagcta aagagcgagt ttatcagcac ctagctcaag agacagaacc ttttgttttg
    27361 attggattgt ccttaggtgc tgccctgaca ctagaacttt ccagttacga tttaccaaat
    27421 cttcgagctt tgattctgtc aggatgcccg cttaaattag cagggaatat cctattttat
    27481 cttcagttgc tgatttttaa actacttccc aaaagagtat ttgaaaaaca gggagcagac
    27541 aagactctta tggtcggagt ttctgaggaa ttgaagacac ttgatttaac ggatatagca
    27601 gggacttgct cctatccaac cttactaatt tgtggcagca aagataaacc gaatcttagt
    27661 tctatgaaag ctctccatag actgttaaca gattcgcagt ttcagatcat tcctgatggc
    27721 cctcatgtct tgaataaggc aaaaccaaag gagtttgtag aaaagaccag aagtttcctt
    27781 gaattgctga aataagtttg ataaaataat attgaaatcg atatcataat tatgggagaa
    27841 agaaatgaat aaacgtctat ttttaaaaat gagtctggtg acgttgccaa ttttagcctt
    27901 gttttcacaa ccagttttgg cggaagaaaa catccatttt tcgagctgta aggaagcttg
    27961 ggcaaatgga tactcggata ttcacgaggg agaaccggga tattctgcca agttagaccg
    28021 agaccatgat ggtgtagctt gcgaattgaa aaatgctcct aagggtgctt tcaaagcaaa
    28081 acaatcaacg gtggctcaaa ctaacacaag ttcagcaaca acaagtggtt gggttgagca
    28141 ggatggcgct tggtactatt ttgatgaaaa tggaaatcca gtgaaaaatg catggcaggg
    28201 aaactattat ttgaaatcag atggtaaaat ggcacagggc gaatggattt atgattcttc
    28261 ctatcaagct tggtattatt tgacatcaga tggttcttat gcttacagta catggcaagg
    28321 aaattactat ctaaaatcgg atggtaaaat ggctgtcaat gaatgggttg atggtggacg
    28381 ttattatgtt ggcgctgacg gagtttggaa ggaaggtcaa gcaagtacag cttcttctag
    28441 taatgatagc aatagtgaat attctgctgc tttaggaaag gcaaaaagtt ataattcgtt
    28501 attccacatg tcaaaaaaac gtatgtatag acaattaact tctgattttg ataaattttc
    28561 aaatgatgca gctcaatatg ccattgatca ttagatgatt aacagatact ggtaggcgaa
    28621 aaaaattcct tataattcac ttacgcaaac ctttgcataa gtgaatgaat tttgatatac
    28681 tagtactgta agcattttca attactacac aatgaaaaag gagaatatga tgagtcaaaa
    28741 gattattggg attgaccttg gtggaacttc tatcaaattt gcaatcttaa caacagcagg
    28801 agaaatccaa ggaaaatggt caatcaagac caacattttg gatgagggaa gtcatatcgt
    28861 tgatgatatg attgagtcta ttcaacatcg tttggacttg cttggattgg cagcagcgga
    28921 cttccaaggc attggaatgg gatcaccagg tgtggttgac cgtgacaaag ggactgttat
    28981 cggtgcctac aacttgaact ggaaaaccct tcaaccaatc aaacaaaaga ttgaaaaagc
    29041 tttgggcatt ccatttttca tcgataatga tgccaacgta gcagctcttg gtgagcgctg
    29101 gatgggtgct ggagataacc aaccagacgt tgtctttatg acactcggta ctggtgttgg
    29161 tggcggtatc gtcgcagaag gcaaattgct tcacggtgtt gctggtgcag caggtgagct
    29221 tggtcacatc actgttgact ttgaccagcc aatctcatgt acttgtggta aaaaaggctg
    29281 ccttgagaca gttgcttcag caacagggat tgtcaacttg actcgtcgct atgccgatga
    29341 atacgaaggc gatgcagcct tgaaacgctt gattgataac ggagaagaag taactgctaa
    29401 gactgtcttt gatctcgcaa aagaaggaga cgaccttgct ttgattgttt accgtaactt
    29461 ctcacgttac ttgggaatcg cttgtgctaa cattggttca atcctaaacc catcaacaat
    29521 cgtcatcggt ggtggtgtgt cagctgcggg agaattcctt ctacaaggtg ttcaaaaagt
    29581 ttacgatgaa aatagtttcc cacaagtacg cacatctact aaattagctc ttgcaactct
    29641 aggaaatgac gctggagtta tcggagcagc atcacttgta ttgcaataag ataatgagag
    29701 aggaaaatgc taacctagag ttagtaatgt tcctctttta ttttttatgc tatactagtt
    29761 aggacaatga atgaggtgaa acgaaatgac aaaagcagat acgattttta aagagaatat
    29821 tgaacgcatt ctaaaggaag gtgttttctc agagcaggct cgtcctaagt acaaggatgg
    29881 gactgttgct aattccaagt atgtaactgg tgccttttcc gagtacgact tatctaaagg
    29941 agaatttcct atcacaactt tgcgtcccat tgcaatcaaa tccgccatca aagaagttct
    30001 ctggatttac caagaccagt caaatagctt agaagtgctt aatgacaagt acaatgttca
    30061 ctactggaat gactgggaag ttggagacac gggaaccatt ggtgagcgct atggtgccgt
    30121 tgttaagaaa cacgacatta tcaataagct tctcaaacag ttggaaacca atccttggaa
    30181 ccgccgcaat attatttcgc tctgggatta ccaagctttc gaagaaacag atgggctgct
    30241 cccgtgcgcc tttcagacca tgtttgatgt tcggcgtgtt gatggggaaa tctatctgga
    30301 tgcgaccttg acccagcgct ccaatgatat gctggtggcc caccacatca acgctatgca
    30361 gtatgtggct ttgcagatga tgattgccaa acattttggc tggaaggttg ggaagttctt
    30421 ctacttcatc aacaacctcc atatctatga taatcaattt gaacaagctc aggaattgct
    30481 ccgtcgggag ccgtcaaact gccaaccacg cttggtttta aatgttcctg atgggactaa
    30541 tttctttgat atcaaagcag aagattttga gttggtggat tatgaccctg ttaagccaca
    30601 gttgaagttt gacctagcta tttaaaagaa tagaaaaaag aagttgagaa taatcccaac
    30661 ttcttttgtt tcttaacgtg atacgcggcg acgagctgct tttttacggt tttcttcgat
    30721 gaaagctgct ttttgctctt ctggttcgat tactttcttt ttaaatgcgt atactgcacc
    30781 tgcaacggca gcgacagttc ctgcgacacc tgttacaaga cctttagcga atcctttagc
    30841 catgagtctt cctcctttat attctcaatc agccagcctc ctcaagaggt cacatttttc
    30901 tgactgacct ttttgtgtta taataatagt aacgaaaaaa tgggaatttt tcaaggaaaa
    30961 aagatgaaaa caaaaataat tgtgattgtt ggaccgactg ctgttggaaa gacggctcta
    31021 gctattgaag ttgcaaagcg ttttaatggc gaagtggtta gtggagatag ccagcaagtc
    31081 tatcgaggac ttgatattgg gacggccaag gctagtccag aagagcaggc agctgttcct
    31141 catcatttaa tcgatgttag agagataacc gagtcttact cggcttttga ttttgtttca
    31201 gaagctaaga tgactattga gggtattcac aaccgtggca agctagccat tatcgccggt
    31261 gggactggac tttatatcca gagcttgcta gaaggttacc acctaggtgg ggagactcct
    31321 catgaggaga ttttagctta tcgagctagt ttggagccat attcagatga ggaattagcc
    31381 catttggtgg atcaagcagg ccttgagatt ccccagttta atcgtcgtcg tgctatgcgt
    31441 gccttagaaa ttgcccattt tggtcaggat ttggaaaatc aagagacctt gtatgaaccg
    31501 ctgattatct gcttggatga tgaacgtagt caactttatg agcgtatcaa ccaccgagtg
    31561 gatttgatgt ttgaggctgg gcttttggat gaggccaagt ggctgtttga ccattcccct
    31621 aatgtacagg ctgctaaagg aattggctat aaggaactct ttccttattt ccgtggagag
    31681 cagaccttgg aggaagctag tgagagtctc aaacaggcga cccgtcgttt tgccaagcgt
    31741 cagctgacct ggttccgtaa tcgcatgcag gtcacctttt atcagattgg agaatctggt
    31801 gtacaagacc gcattttaag ccagatagag gagtttttag atgattgaaa cggagaaaaa
    31861 agaggagcga gtcctgctga ttggtgtgga attgcagggt atggacagtt ttgacctctc
    31921 catggaagaa ttggctagtt tagcgaaaac ggcaggggca gtcgttgtag atagctacag
    31981 acaaaaacgt gaaaaatatg attccaagac cttcgtcggc tctggtaagt tggaagagat
    32041 tgcgcttatg gtggatgcag aagaaatcac tactgtcatc gtcaacaacc gtctgacccc
    32101 aaggcagaat gtcaatctag aggaagttct cggtgttaag gtcattgacc gtatgcagtt
    32161 gattttggat atctttgcca tgcgggctcg aagccatgaa gggaagctcc aagtccacct
    32221 agcccaactc aaataccttt tgcctcgctt ggttggtcag gggattatgc tcagccgtca
    32281 ggcaggggga attggttccc gtggtcctgg tgaaagccag ctggagctga accgtcgtag
    32341 cgttcgcaat caaatcacgg atattgagcg ccagctcaag gtggttgaga aaaatcgggc
    32401 gactgtcaga gaaaaacgtt tggagtctag cacttttaag attggtttga ttggttatac
    32461 taatgctggg aaatcaacta tcatgaacat cttgaccagt aagacccagt atgaagcaga
    32521 tgagctcttt gcgactctgg atgcgacaac caagagtatt catctgggag gcaatctcca
    32581 agtaactttg acagataccg ttggctttat ccaagatttg ccgacagagt tggtgtccag
    32641 tttcaagtca accttggaag aaagcaagca tgtggacctt ctggttcatg ttatcgatgc
    32701 tagcaatcct taccacgagg agcatgaaaa aacggttctc tccatcatga aagacctgga
    32761 catggaagat attcctcact tgacgcttta taataaagcg gatttggtgg aggatttcac
    32821 gcctacccaa acgccatata ccctcatttc tgccaagtct gaggacagtc gtgaaaactt
    32881 gcaagcatta ttgctagata agattaagga aatttttgaa gcatttaccc tgcgagtgcc
    32941 tttttcaaag tcctacaaga ttcatgattt agagagtgtt gcaattctgg aagaacgtga
    33001 ttatcaggaa gacggcgaag tgattacagg ctacatttcc gagaaaaata aatggaggtt
    33061 agaagaattt tatgactgat attaaaactt tggctctaaa gtatggaggt tatacaagtc
    33121 tggacaaggt ctatctggat cagcttctag ctggtaaaac agagcaggag cagttaacac
    33181 ttattacacc tccgccaagt gttgttaatg cctactttgc agaactctat cagaaaaaaa
    33241 gtcctcaggc tgcgacggat tattttgcag aactcagtca ggaactgaat ctttacaata
    33301 ctgagccaag tttcacccta gaaaataagc cttttattcg gcttaatctg tctggtaaat
    33361 cctttggttt ttgttatgag agtgacggct ttggtcggat tttctctgaa aatgaggaga
    33421 aaatctcgga tgacttgctc tttgaaattg cgcaaatttt ctcccatcaa ttagtctttg
    33481 aagagtctgg taagatttac atgaagcctg tcaaggacga ggaagttgtc agcctagaaa
    33541 gtctcacagc tttgactgat ttggaaagat tggctgatgg tcgaaaacgt ctcaaaggct
    33601 acagccaaga ggatttattg caagaagctg ctgctttttc tggcaaacgc tatttccgat
    33661 cggaaaaccg cacagccatg ttatatattg attaattaga aagtatcgaa tggatattca
    33721 atttttagga acgggggctg gtcagccctc taaagcccgc aacgtttcaa gtctcgccct
    33781 gaaacttttg gacgagatta acgaagtttg gctctttgac tgtggagaag gtacgcaaaa
    33841 tcgcattctg gaaaccacaa ttcgaccacg taaggtcagc aaaatcttta ttactcatct
    33901 gcatggagac cacatttttg gtttgccagg tttcctttct agccgtgcct ttcaggccaa
    33961 tgaagagcag acagatttgg aaatctacgg acctcaagga atcaagtcat ttgtcttaac
    34021 cagccttcgt gtgtcaggtt ctcgtctgcc ctaccgcatt catttccatg agtttgacca
    34081 agattctctg ggtaaaattc ttgaaaccga taaattcact gtgtatgcag aggagctgga
    34141 ccacactatt ttctgtgttg gctatcgtgt catgcaaaag gatctagaag ggacgctgga
    34201 tgctgaaaaa ctcaaggctg ctggtgttcc gttcggcccg ctttttggta aaatcaaaaa
    34261 cggccaggat cttgttttgg aagacggaac tgaaatcaag gcagcagact atatctcagc
    34321 gccacgtcca ggtaagatta tcactatttt aggagacact cgaaaaacgg atgccagtgt
    34381 gcgtctggct gtcaatgcag atgtcctagt tcatgagtcc acttatggca agggtgatga
    34441 aaaaattgct cgtaaccatg gtcactcaac taatatgcaa gctgcacaag tagcggtaga
    34501 agcaggtgcc aaacgcctcc tactcaacca tatcagtgcc cgtttcctct caaaagatat
    34561 aagcaaactc aagaaggacg ctgccacaat ttttgaaaat gtccatgtgg tcaaagactt
    34621 ggaagaagtg gaaatctagc agtcacagaa aggataagta tgcctactat tctcattacc
    34681 ggagctagcg gtggtctagc tcaagaaatg gtcaaactcc tgcccaatga ccaactcatc
    34741 ttgcttggta gaaataagga aaaattagcc caactctacg gaaattattc ccatgcagaa
    34801 ttgattgaaa ttgatattac cgacgattca gccctagaag ctctggtaac tgatctttat
    34861 ctccgctatg gcaagattga tgtcttgatt aacaacgctg gttacgggat ttttgaggga
    34921 tttgaccaga ttgctgataa agatattcac cagatgtttg aggtcaatac ctttgccctg
    34981 atgaatctgt ctcgtcacct tgcggctcgt atgaaggaaa gcagcaaagg gcatatcatc
    35041 aacatcgtca gcatggcagg tctaatagct actggcaagt ctagtcttta ctcagcgacc
    35101 aagtttgcgg ctattggttt ttcaaatgct ctgcgactcg aacttatgcc ctatggagtc
    35161 tatgtgacaa cagtcaatcc aggaccaatc cgtacaggat ttttcgacca agccgaccca
    35221 gatggaactt atcttaaatc ggttgaccgc ttcctgctag aaccagatgc agtggctaaa
    35281 aagattgtca agattatagg aaaaaacaaa cgagaactca atctcccgat tttgttgaac
    35341 ctagctcata agttttatac cctctttcct aagctagctg ataagttggc aggggaaact
    35401 tttaattata agtaaaaaga accaatgtgc aggttgttgc tagcctacat attggttctt
    35461 atcatttctc aactatcaaa ctgaacttct tctaggaggt agtctataaa gcgttcgccc
    35521 atcttagata ggctggtttt ctcatgctgg atatagacca gctcaatcgg gtcatcaata
    35581 tccagtggga tagaaacgat attgtctccg tttaggttgc tgttcaaaat ccctgtcgca
    35641 atggtataac catccaaacc aatcaagaga ttaaagaggg tggcacggtc actgaccaca
    35701 atggatttct tgtggtgttc ttgagaaaga atctcttctg aaaagtagaa ggagttgtgc
    35761 gtcccttggt catagctgag gtaagggaaa ttctccaaat cagacagttt caccttgtct
    35821 ttctttgcca gagggttggt cttgctgaca aagatatgtg gttgcgctgt gaagagatgg
    35881 tgggctagca ggtgattgtc atccagcatc ttggttaaaa catcacggtt gtaactgttt
    35941 aagaagagga ccccgacctc actgcggaag ttcttgacgt cgtcgataat ctcccaagtc
    36001 cgagtttcac gaaggaagag ttcgtatttc tccatatcgc ttttcttgag caaagagaca
    36061 aaggcattga ccacaaaggc atagtgttga gacgaaacgc taaatagttc gcggtgggcg
    36121 acaggatttt tatagcgttc ctccagaagc tgggtctgct cgacaacctg acgggcatag
    36181 gagagaaact ccatcccatc acgggtcaag gtgattccct tgggattgcg gataaagatc
    36241 tcaatgccca tttcattttc caaatctcgc actgcattgg agagacttgg ctgagtgata
    36301 aagagttgct tggctgcctc attcatggag ccagtttcga cgattttgat aatataatgt
    36361 aattgttgaa ttctcatgtt tttattgtac catacttgcc ttagaatcgc taaagaagat
    36421 gggaaaataa ggagcattgt gtgagtctct tcttgaaaaa aacacaaaat tttgctagaa
    36481 tgagtcttat gaaaacattc tatgatgtgc agcaattcct caaacgattt ggtattattg
    36541 tttacatggg aaaacgctta tatgatattg aactgatgaa gttggaacta tctcggattt
    36601 acgatgcagg gttgatggat aaattagact atctagaggc agaagcagtt cttcgcagag
    36661 agcacaaggt agaattggat tatattgaga aaaatggaga aaagaactaa tggttacttg
    36721 gattttgtgg gcacttatac tagcaatgtt ggcgtggatg ggctttaact atcttcgtat
    36781 tcgccgtgcg gctaaaattg tggacaatga ggagtttgaa gccttgattc gtacgggtca
    36841 attgattgat ttgcgcgacc cagcagaatt ccacagaaaa catatccttg gtgcacgcaa
    36901 tattccttca agtcagttga aaactagtct tgcagccctt cgtaaagata aacctgtcct
    36961 tctctacgaa aaccaacgtg cgcaacgagt tacaaatgca gctctttact tgaaaaaaca
    37021 aggtttttct gagatttata tcctttctta tggcttggat tcttggaaag ggaaagtgaa
    37081 gactagctaa ccttatttcc aaattagttg aatgatagga tgaagctatt gttttatagt
    37141 ataattaatt tgtataaaat ttaaggagtt cttagaacat gaaagaaaag aagaaaatcc
    37201 actttttgtg ggtatcttgt cgattattct tggtttgtta tttccaattg taggcctgat
    37261 tttgggtatc ataggattgg tcttggctat ttcataccaa aaagagtcac aattagacta
    37321 taaaatagaa aagattctta atatcttagg aattgtgatt tctgtagtta actggattgt
    37381 agcaatcgcc ttaatttttc ggtaaataaa agaaaaaagc tttagtttta aagctttttt
    37441 tattttatcg actcatctct aaatagtttt taatgatttg taactctttt tggttcaaag
    37501 ggcggtagtt accttctgct aaatctgggt tcaatgtgaa gtccccaaat tggattctct
    37561 tgaggctagt caccttaaca ccaaccgaga ggaacatttt cttgatttga tgaaattttc
    37621 cttctgaaat ggtgatagag gcttgactga tggagggact tgcagataga atctctagtt
    37681 ttgcgggttt acagacagta ccatctaaaa agacaattcc cttttgaaag gcttggatat
    37741 ggtcaggtgt tagaagtcca ttaacctcaa cttgataagt cttatcgaca tgatattggg
    37801 gatggaggag ttggaagccc aagggaccgt tatcggtcaa gagaaggagt cctgttgtat
    37861 ctcggtccag tcggccgacg gcatagagct tgtcagactg gatgtcagat ggaagcaggt
    37921 ccatgacggt cggaagttcc ttgtctttgt tggctgtaac gacaccagca ggtttatgaa
    37981 gcataagata ggtgtgttca tagccttgaa tgattcgatc ctgaaaaagg agttcttgta
    38041 gtcctgtatc gatattttga gctagggagc gggctgggca accatcgact agaatttccc
    38101 ctctgaggag tgcttgtttc atggcctttc ggctgatttt ttcttgagct aataaattat
    38161 ctaaacgcat actgtgccta tcttatcata agtcgcttgc tttgaaaagg cttgacgtga
    38221 aaagaaaagc ggagtgaaaa catttacact tgcttgagtt atgttattta tttgaaatta
    38281 tggtataatc gttcagttag aaaataaatt ttgaatatta tagaggaaat catgacaaaa
    38341 ttaagagaag atatccgtaa cattgcgatt atcgcccacg ttgaccacgg taaaacaacc
    38401 ctcgttgacg aattattgaa acaatcagaa acgcttgatg cacgtactga attggcagag
    38461 cgtgctatgg actcaaacga tatcgaaaaa gagcgtggga ttaccatcct tgctaaaaat
    38521 actgccgttg cttacaacgg aactcgtatc aacattatgg acacaccagg acacgcggac
    38581 tttggtggag aagttgagcg tatcatgaaa atggttgacg gtgttgtctt ggtcgtagat
    38641 gcctatgaag gaaccatgcc acaaactcgt ttcgtattga aaaaagcctt ggaacaagac
    38701 cttgtcccaa tcgtggttgt taacaaaatc gataagccat cagctcgtcc agtagaagta
    38761 gtggatgaag tcttggaact tttcatcgag cttggtgcag atgacgacca gcttgatttc
    38821 ccagtggttt atgcttcagc gatcaacgga acttcttcat tgtcagatga tccagctgac
    38881 caagaagcga ctatggcacc aatctttgac acgattatcg accatatccc agctccagta
    38941 gataactcag atgagccttt gcagttccaa gtgtcgcttt tggactacaa tgacttcgtt
    39001 ggacgtatcg gtatcggtcg tgtcttccgt ggtacagtta aggttgggga ccaagttacc
    39061 ctttctaaac ttgacggtac aactaaaaac ttccgtgtta caaaactctt cggtttcttt
    39121 ggtttggaac gtcgtgaaat ccaagaagcc aaagcgggtg acttgattgc cgtttcaggt
    39181 atggaagaca tctttgtcgg tgaaaccatc actccgacag atgcagtaga agctcttcca
    39241 atcctacaca tcgatgagcc aactcttcaa atgactttct tggtcaacaa ctcaccattt
    39301 gctggtaaag aaggtaaatg ggtaacctct cgtaaggtgg aagaacgctt gcaggcagaa
    39361 ttgcaaacag acgtttccct tcgtgttgac ccaactgatt caccagataa atggactgtt
    39421 tcaggacgtg gagaattgca cttgtcaatc cttatcgaaa caatgcgtcg tgagggctat
    39481 gaacttcaag tatctcgtcc agaagttatc gtaaaagaaa tcgacggtgt taaatgtgaa
    39541 ccatttgaac gtgtacaaat cgacactcca gaagaatacc aagggtctgt tatccaaagc
    39601 ctttctgaac gtaaaggtga aatgttggat atgatttcaa ctggtaatgg tcaaactcgt
    39661 ttggtcttcc ttgttccagc gcgtggtttg attggatact caactgagtt cttgtcaatg
    39721 actcgtggtt acggtatcat gaaccatacc ttcgaccaat acttgccatt gattccaggg
    39781 gaaattggtg gacgtcaccg tggtgccctt gtttctatcg atgctggtaa ggctacaact
    39841 tactcaatca tgtctatcga agaacgtggt acgatctttg tcaacccagg tactgaggtt
    39901 tatgaaggaa tgatcatcgg tgaaaactct cgtgaaaatg acttgacagt taacatcacg
    39961 aaggcaaaac aaatgaccaa cgttcgttca gctactaagg accaaacagc tgttatcaag
    40021 acgcctcgta tcttgacact tgaagagtct cttgagttct tgaacgacga tgagtacatg
    40081 gaagtaacgc ctgagtctat ccgtttgcgt aaacaaatcc ttaacaaagc agagcgtgag
    40141 aaagctaaca agaagaaaaa atcagctgaa taagagctag aaagagagaa agatggtcta
    40201 tttagtccta ggaattttac tgctcctact ctatgtattt gcgacaccag aaagcattaa
    40261 agggactgtc aatatcgtcg ctatggtatg tattttagtg gcactcttga ttttattggt
    40321 tctatctttt ctgaaaattt ttcaattacc aacagaaata ttcctagcaa tagccatgtt
    40381 gatcctagct tactttagtg ttagagacat cacactcatg ccagtcaaaa aaagtaaaag
    40441 aagataaaaa agagagtttc ggctctcttt ttgctaggaa ttttacaata gtttgaattg
    40501 ctttttagtt aacaccatct cttttacttg tatagtatga atgggaaaaa cttgaataaa
    40561 attcgttaat ggcatctatt ttatatttgg ttagtactgg ttgcatatta ttttgaaata
    40621 aaactactat ataatattat tcatagagat aatatagtaa aacatctaaa aaattcgatt
    40681 ttttccttga caacaaataa taaaaatgat atttttatca cataagaaag cggtttcaac
    40741 aaaagagaga ggagggttta ttttgttgca ttgttgctca aggtgtgctt tttttgaaag
    40801 aaaaaataaa atgaaaacga aaaaacatag attacttgct ctagctctta tttcaagttt
    40861 tacattattg ggagctgcat cagctgctgt acaatatcca gatggaggag tatggacgta
    40921 tggagaaggt tcaggaggtg gttgggcttt ttcaaattac tatcatggta aaaaatatca
    40981 ttattcatcc atcgtgagca gatgggatgg tcattctgat aaaggagaag ctcctgctgg
    41041 aaaaacctcc tatgcatgga tttggactaa atggggagaa caagtagcat tttactgtga
    41101 ttatgactaa tcgttcattg atttaaatcg ttccccttgt aagaaattac agggggaatt
    41161 ttgtttacaa ggagtctact atgaagaaac tattcatatt attatcaact ttttttctca
    41221 gcttcttcct tgcttggatt attgtcttac gtgcgccaca atatttatat gcaagctatg
    41281 attccgtttc tttacttcgt gtcaaaaaag atactcagga accgacgcgt gaggtatttg
    41341 aacaggaatt ggagaatttt gcaaactcag aacagagttt aatagctaga agaattgtag
    41401 agccgagtaa ggatgggacg actcacttta cttatgcaac ttatggtcag ggaactttac
    41461 caaaagaatt ccaagaagct agccaagaaa gtcgtgaacg tagtgatccg ctaaatagtt
    41521 atctcctttt gtcaggctcc ttgacgaaag aaaagcttgc cgataaatta ggagatttgg
    41581 gttataaagc aagtgctgac cgaaagatac cgccctattt tcttgctttt cgaatattac
    41641 taaatcccct tattttaatt agtttagcaa tatttggctt atctttcttt gctttagtga
    41701 ttatcactcg gattaaggaa atgagagcag caggtataaa actcttttct ggtcagactc
    41761 tcttatccat catggggcat tctttatcga ctgatatcaa atggctcctt ctatcagccc
    41821 tcctttcctt cctaggtggg ggagtcgttc tttttagtca aggtttgttt tatcctatct
    41881 tgttagccac ctatggtttt gggattagtt tctatctgtt gtttttattg gcgatttcaa
    41941 ttttactaat gcttctttat ctaatgagtt tgaattacaa agcattagtt cccgttatta
    42001 aggggagatt accccttaaa cgcctgatga ttttaaccct attgtgtcag ttggttgctg
    42061 tctttacagt aggatacgct gttaagacag gtttgacgtc ttaccaacga ttgaaagaac
    42121 ttgaaatttc aaaacaagca tggcaggata gagcagacta ttatcaaatt tcttttggct
    42181 taggtgatag aggaaaagat acagaaaatc agagcaagtg gtatgccttt gccaaggaag
    42241 caatcgaaga agaacaagct ctttatgtaa aggataatct gctccatttt gccaatccac
    42301 aaggaaaaaa tgaacaggga gagacactgg atacctatag tccagatgct aatacgctct
    42361 atgttagtcc cagttatttg gacaaggaaa aggtcgtggt agatgctgag accaaacaga
    42421 agttagccca tctccaaaaa ggtgagttta tcctcttgct cccagaacat ttgcgctctc
    42481 gagaagtaga acttaagaaa gtttttgaag aaagattgag ttattatgga aaatctggtg
    42541 aggaggcaag tgctcctttg gattatgaga tgaaagcgca cgttagttat ctttcaatgg
    42601 gagaaaagcg gtttgtttat aataacggtg agaatcccgt atctactcag tatttgactg
    42661 atccgatttt agtggtattc acgccgactt ctacaggtga tagttttata tccttatcta
    42721 gttggtctat caatgctgga aaacaactct ttatcaaagg atatgagagt gggctagaac
    42781 tcttgaagaa agttggaatt tatgagcaag tatcctatct taaagaagga agaagtgttt
    42841 atttaactcg ttataatgaa gttcaaactg aaacagcaac tttaatctta ggagctattg
    42901 ttggaatagc cagttccttg ttactctttt attctgtcaa tcttctatat ttcgagcaat
    42961 tccgccgaga tattttgatt aaacgaattt caggtttacg attttttgaa acacatgctc
    43021 agtatatggt tagtcaattt gccagttttg tatttggtgc tagtctcttt attttaagca
    43081 gtcgagactt ggtgattggc ttgctcactt tattagtctt tctagctagt gcagttctga
    43141 cgctttaccg tcaagcgcag aaagaatctc gtgtttctat gacaattatg aaaggaaaat
    43201 aggatgattg aactaaagaa tatatctaaa aaatttggaa gccgtcagct attttcagat
    43261 acaaatcttc attttgaagg tgggaaaatt tatgccttaa tcggtacaag tggctgtggt
    43321 aagacaacac tcttgaatat gattggacga ttagagccat atgacaaagg gcaaatcatc
    43381 tatgatggca cttctcttaa ggacatcaag ccttctgttt tctttagaga ttacttggga
    43441 tacttatttc aagattttgg cttaattgaa agccaaaccg tcaaagagaa tctcaatctg
    43501 ggtttagttg gtaaaaagtt gaaggaaaaa gagaaaatct ctttgatgaa acaagctcta
    43561 aaccgtgtaa acctctctta tttggatttg aagcaaccta tatttgagtt atcaggagga
    43621 gaagcacaac gtgttgcatt agcgaagata attttaaagg atccgccttt gattcttgca
    43681 gatgaaccaa ccgcttcctt agaccccaaa aactctgagg aattactttc catcctagaa
    43741 tctttaaaaa atccgaatcg gaccattatt attgcgaccc acaatcctct gatttgggag
    43801 caagtggatc aggtcattcg agttaccgat ttatcacata gataatatgg taagattcag
    43861 ttagaagaaa gagtcacaaa cacactttgt ggctttttta tttccataaa aatggtaaaa
    43921 tagtaggagt agaaatggag ttcgagacat gaaagtaata gatcaattta aaaataagaa
    43981 agttcttgtt ttaggtttgg ccaagtctgg tgaatctgca gctcgtttgt tggataagct
    44041 aggcgccata gtgacagtaa atgacgggaa accattcgag gacaatccag ctgcccaaag
    44101 tttgctggaa gaagggatca aggtcattac aggtggccat cctttggaac tcttggatga
    44161 agagtttgcc cttatggtga aaaatccagg tatcccctac aacaatccca tgattgaaaa
    44221 ggctttggcc aagggaattc cagtcttgac tgaggtggaa ttggcttatt tgatttcaga
    44281 agcaccgatt attggtatca caggatcgaa cggtaagaca accacaacga ctatgattgg
    44341 ggaagttttg actgctgctg ggcaacatgg tcttttatca gggaatatcg gctatccagc
    44401 tagtcaagtg gcccaaactg cgtcggacaa ggacacgctt gttatggaac tttcttcttt
    44461 ccaactcatg ggtgttcaag aatttcatcc agagattgcg gttattacta accttatgcc
    44521 aactcatatc gattatcatg gattatttga ggaatatgta gcagccaagt ggaatatcca
    44581 gaacaagatg acagcagctg atttccttgt cttgaacttt aatcaagact tggcaaaaga
    44641 cttgacttcc aagacagaag ccactgttgt accattttca acacttgaaa aggttgatgg
    44701 agcttatctg gaagatggtc aactctactt ccgtggtgaa gtagtcatgg cagcgaatga
    44761 aatcggtgtt ccaggtagcc acaatgtgga aaatgccctt gcgactattg ctgtagccaa
    44821 gcttcgtggt gtggacaatc aaaccatcaa ggaaactctt tcagccttcg gtggtgtcaa
    44881 acaccgtctc cagtttgtgg atgacatcaa gggtgttaaa ttctataacg acagtaaatc
    44941 aactaatatc ttggctactc aaaaagcctt gtcaggattt gacaacagca aggtcgtctt
    45001 gattgcaggt ggtttggacc gtggcaatga gtttgacgaa ttggtgccag atattactgg
    45061 actcaagaag atggtcatcc tgggtcaatc tgcagaacgt gtcaaacggg cagcagacaa
    45121 ggctggtgtc gcttatgtgg aggcgacaga tattgcagat gcgacccgca aggcctatga
    45181 gcttgcgact caaggagatg tggttcttct tagtcctgcc aatgccagct gggatatgta
    45241 tgctaacttt gaagtacgtg gcgacctctt tatcgacaca gtagcggagt taaaagaata
    45301 aaatatgaaa aaaattgtct ttacaggtgg ggggacggtt ggacacgtta ccctcaatct
    45361 tttgttaatg cccaagttca tcgaagatgg ttgggaagtc cactatatcg gggacaagcg
    45421 tggtatcgaa caccaagaaa tccttaagtc aggtttggat gtcactttcc actccattgc
    45481 gactgggaaa ttgcgtcgct atttctcttg gcaaaatatg ctggacgtct tcaaagttgg
    45541 ctggggaatc gtccaatcgc tctttatcat gttgcgactt cgtccacaga cccttttttc
    45601 aaaggggggc tttgtctcag taccgcctgt tatcgctgcg cgtgtgtcag gagtgcctgt
    45661 ctttattcac gaatctgacc tgtctatggg cttggccaat aaaatcgcct ataaatttgc
    45721 gactaagatg tattcaacct ttgaacaagc ttcgagtttg gctaaggttg agcatgtggg
    45781 agcagtgacc aaggtttcag atcaaaaaaa tccagaacca gatgaattgg tggatattca
    45841 aagccacttt aatcataaat tgccgactgt attgtttgtt ggcggttctg caggtgctcg
    45901 tgtctttaac caattggtga cagaccataa aaaagaacta acagagcgct acaatattat
    45961 caatctaact ggagattcta gcctgaatga gttgagccaa aatctttttc gtgttgacta
    46021 tgtgaccgat ctctatcaac ccttgatgga attggctgat attgttgtga cacgtggtgg
    46081 tgccaatacg atttttgagc tcttggcgat agcaaaattg catgtcattg tgccgcttgg
    46141 tcgtgaagct agtcgtggtg accagattga aaatgcagct tactttgtta agaaaggcta
    46201 tgcagaagac cttcaagaaa gcgatttgac cttggatagt ttggaagaga agctttctca
    46261 cttactaagt cacaaggaag attaccaagc taagatgaaa gcttctaagg aattgaaatc
    46321 tctagcagat ttttatcaat tgttgaaaaa agatttatca taaggaaagt aaatgtcaaa
    46381 agataagaaa aatgaggaca aagaaaccct cgaagaattg aaagagttat cagaatggca
    46441 gaaacgaaac caagaatatc taaaaaagaa ggctgaagaa gaggcggctc tagctgagga
    46501 gaaggaaaag gaaagacaag ctcgaatggg agaagaatct gagaagtcag aggataaaca
    46561 ggaccaggag agtgaaacag accagaaaga ttcagaatca gctaaggaag agtctgaaga
    46621 aaaagtagca tcctcagagg ctgacaaaga gaaagaagag aaagaagaac cagagtctaa
    46681 agagaaggag gaacaggata aaaagcttgc taaaaaggct acaaaggaaa aaccagccaa
    46741 agcaaagatt cctggtatcc atatcttgcg agccttcacg attttatttc caagtctgct
    46801 tttattgatt gtctctgcct acttgctcag tccttatgcg accatgaaag atattcgtgt
    46861 tgagggaacg gtgcaaacta cagctgatga tattcgacag gcttcaggca ttcaggattc
    46921 ggattatacg attaaccttc tgctagacaa ggcaaaatat gaaaagcaga ttaagtctaa
    46981 ctattgggtt gaatcagctc aacttgtcta tcaatttcca actaagttca ctattaaggt
    47041 caaggaatat gatattgtgg cctactatat ttctggtgaa aatcattatc ctattctttc
    47101 cagtggtcag cttgagacta gttctgtgag tctgaacagt ttaccagaaa cttatttatc
    47161 agttctcttt aatgatagtg aacaaatcaa ggtttttgtc tcagaacttg ctcaaattag
    47221 cccagaactc aaggcggcta tccaaaaggt ggaattagcc ccaagcaagg tgacatccga
    47281 tttaattcga ttgaccatga atgattcgga cgaagtcttg gttcctctat ctgaaatgag
    47341 taagaaactg ccatattaca gtaagattaa gccacaattg tcagaaccga gtgtgattga
    47401 catggaagct ggagtctaca gctacactgt agcggataaa ttgattatgg aagcagaaga
    47461 aaaagctaaa aaagaggccg aagaagctga gaaaaggcag gaagaggaac gtaaacgttt
    47521 agaagaagaa cagaaaaaac aagaggaaga gagcaatcga aaccaaacga cccagcgctc
    47581 atcgcgtcgc taggttttac cttttctcct atagctcttt agttgccatg tttttacgtt
    47641 taatatttga catctgtttt tctttatgtt acatttgcaa tgtaatcgat tacaaaaaaa
    47701 aattttatga agaaggtaac acatatgtca gatgaagtat tcttatttga agaaattgaa
    47761 gaaatcgtag cccctacaga tggtgaattt tgggggggag ttttacttgg aactggggta
    47821 gttctcttaa ttggagtagc ctgttgttaa aaagataggg agtgataatc atgcaagata
    47881 actttttatt tgaggaaatt gaagaaattt cagtaccagt tagtgatttt tcagctggac
    47941 ttgcaacagg tatcggattt ggtttagcaa tccttgctct tgctggttgt tgaagtttgt
    48001 tcacttacta atatcaagct ttttcaattt cattttagac agtcatttga attttccgta
    48061 ttagtcttgc agcaagagat taatagaatt agtcattatt ttattgattg cggactgagg
    48121 gactagagta tgttttactt agcccctctt ttatttatta aaggttaggt ttgttatgag
    48181 aattgttgat aagattaaga tattacctac tccttatgag ggacagtatc atctatatat
    48241 accatccagt aagaaacatg tattagttgg gaaacaggaa aaaaatggtt agagcaacta
    48301 ataggtcaag aatttaccat atcggactta ttagtgttag tagggaagaa atattattta
    48361 agattttata ttgttcttag taaaatggga cttatagatt ctatgaaatc acgaaaaaag
    48421 ataaatattt ttaaaatatc ttgggacttt aatataacat tatctgaaaa attaaactat
    48481 aaaagattta ataagaattt tgaaaaaatc ctatttggta ttttcccatt ggctcttatt
    48541 ttgcaatgtt ttattatgta tatttatagg gcggatattt tgactaattt ccaaaacttt
    48601 ccaataacgc taaacattct tttattttgg ttgcttgttg ttattatatc tgcaacgata
    48661 catgaattta gtcatgcaat aattgctaat aattatcaaa ttgagatccc aaattgtgga
    48721 ataatgctac ttttgtttaa tttggctttt tttgtagatt taagtggggt gcaattccta
    48781 aaaaataaaa aacaattttt gaaaattatg ttagcaggaa ttgcttcaaa ttcgatttta
    48841 tcacttacag gtttacttgt tttattgttc acatcgtata aattgcttgg actcttattt
    48901 tttatcatta acttaggtat gatttttatt aattcaattc ctttttttca gtatgatagt
    48961 ggtattattt taagatactt gaattctaac aataataact tgaattttca atatatagtt
    49021 caacttttaa tagcatttgt tattatttat ttccctttga gtcaactatt acaatttttg
    49081 acacctaata ttattgttcg tagtatagga ggggtagttg tttctatact gctttctata
    49141 ttatatatga gaggaaggac gaaatatgtt ctacgtaaat agttatgttt ttgcttataa
    49201 aaaagaaggt ataatgtatt tgcgtggtcg gagtatgcgg gaaatagcta tagaacctca
    49261 aatttcgcaa gaatttatca acgatctatt taatagttgt aaggaactat tagagataga
    49321 agaagtatta ggtagtaaac taacatttga attattaaat gaacaaattt taatttcgga
    49381 tgagatagat attgatagta gatattctag aactaaaggt tactattcgt tattttataa
    49441 tgaagagtat aataaaatac agaataaaac agtattagta ttaggagccg gagccttagg
    49501 atgttatata tctctaagtc taagtatgta tggagtgagg aaacttattg tcgctgatta
    49561 cgatataata gaaccatcaa atttaaatag gcaaattctt tatacagagt cggatgttgg
    49621 taaggagaag attaatgttc tttctcaaaa aatacacaag tataattcag atgttcaggt
    49681 agtacctatt tctattaaag tttcttcagt agaagaatta gaaaacattg ttgcggaata
    49741 tgggagtata gattttatcg ttaaagcaat tgatacgccc attgatatta taaaaattgt
    49801 caatcaattt gctgtatcgc ataagatatc ctacatatca ggagggttta atggatgcta
    49861 tcttattatt gataatatat atatccctac catcggttct tgctttgctt gtcggaatat
    49921 aaacaaagat ataaataagt acactttatc tgataagaca aagtggccga ctacaccaga
    49981 gatgcctgct attttgggag ggataatgac taatttaata attaaaatat ttctgggatg
    50041 ttataatgaa atcctaatag ataacgctta cgtttataat atgagaaatc atgctctaag
    50101 tcaagaaaaa tatgttctgg aaaacggaga atgtccaatt tgtaaaaaaa ataataaagt
    50161 gaaagataac aatattagag cgaaaacatt tattcgttca gtttgttttt gcttattatc
    50221 aggaggagta gcttttttat ctgctattgg gcagttcact gttatagaaa cacaattaat
    50281 agtattgttc ttgggtatta tttttgctat atattatgct tactacaata aaaatattca
    50341 aacatcattg gaaaatatag tatggctttt ttcatcgttt gagattttat ttttgcttgt
    50401 taattttaga acatttattc agttaccagt ggatattttt attagtatga taatattttt
    50461 aatgctgtgg atatttatta tgttaggtat agtgtgtctt agttattata taactttatt
    50521 atttagcaag gaggcttagt atgtttaaaa aaataggtat aatgagcatt tgcatatata
    50581 taattatttt atactgcttg agaatgtatc atattatcaa taatattgaa acaatcttgc
    50641 taacggttat atgcttaatg ttattgtttt ttttaagacg tttatttgat aaagctaagt
    50701 aaatagatgt taagtaaaaa tgtagaatat aaaggagggg caatgagtat gattgaagtt
    50761 agccatttat caaaaagttt tggtgataaa atagctttaa atgatataag tttcactgtt
    50821 aaagaaggtc agatttttgg atttttagga ccatctggtt ctggaaagac cacaacgatt
    50881 aatattctga ctgggcagct ccttgccgat aaaggacaat ctattatttt gggacaaaaa
    50941 tctcaaaatt taacaagcgg tgaattaaag agaattggat tggttagcga tacaagtgga
    51001 ttttatgaga aaatgtctct gtataacaat cttctttttt atagtaaatt ttataatatt
    51061 agtaaattac gtgttgataa tttgttaaag cgagtaggat tatatgatag ttgcaagatg
    51121 gtagcaggaa aattatccac tggaatgagg caacgaatgc ttttagcacg agctcttatc
    51181 aacaaacccg ctgtactctt tctggatgaa ccgacctcag gtctagatcc cacaacttct
    51241 cgaacaattc atgagttaat tttagaattg aaaacagcag ggacaacgat ttttctaacg
    51301 actcatgata tgaatgaagc aactctttta tgtgattatg ttgccttatt aaataaaggg
    51361 aaattagttg agcaaggagc tccttctgaa ctcattcaaa gatataataa agataaaaag
    51421 attaaggtta cagattataa tgggaatcag ataacttttg attttacatc actagaacag
    51481 gtatctcagg ctgatctgga aaatattttt tcaattcatt catgtgagcc tactttagaa
    51541 gatattttta tcacattaac aggaggaaag ctaaatgctt aaacggtttc tggctttggt
    51601 atggttgcgt tgtcaaatta tcctttccaa taagagtatt ttattgcaag ttttagtgcc
    51661 ttttgctttc acatattttt ataaatatct tatggaaaca caggggaagg tcaacgatca
    51721 acaggcatta gttcttttga tgatgtgttt acctttttct ttggctttgg ctgttggaag
    51781 tcctataact attatcttgt ctgaagaaaa agaaaagtac aatttacaaa ctcttctgtt
    51841 gagtggtgtt aaaggatccg aatacatttt atcaacgatg tttcttcctt ttttgctaac
    51901 ttttgtgatt atgggaacta ctcctcttat tttaggagtt acaattgtac atacttttaa
    51961 ttatattaca atcgttcttc taacctcttt atccatcatt ttattctatt tattgatagg
    52021 tttaaccgcg aagagccaag tagtagctca ggttatcagt cttcctgcta tgattttagt
    52081 tgctttctta ccgatgctat ctggtttgga taagacagtt gcgaagataa cagattatag
    52141 ttttatggga ctgtttacta agtttttcac aaaatgggag ggattttcat ggaatgaaac
    52201 tctaattcct aatctaacac tacttatttg gattgttctt ctattaactt taattacgat
    52261 aactattagg aaaaagaaaa tttcttaatt gagttatttt aatgattata aacacaagtg
    52321 ggaaggaaaa atgaactgat ctttttgaca gcaattctac agaatagtct tattgctata
    52381 ttttgatttg agtgtacgaa aaaagaaaaa taacaatagt gctcatacta attgcagaag
    52441 ttttgggtga taagataact gataaattgc aataaaaaat gcaacatttt taaatctcct
    52501 ctataagtgc ttcaaaacct gtcttgtaat ccaagtattt ttggggacgg tgattaataa
    52561 gctagcaaag caccattaag gattttttcg gtaattgttg ccaaatcggt ttaagaaaat
    52621 actcacgaag aagtccattc gcattctcat tacttcccct ttgccaagat gaataggcat
    52681 ccgcaaaata aaacagaatt cccatttgtt caattaaagg gtaacaagca aactcttttt
    52741 ctctgtccta agtgaaagtc tttaactatt cttttggaaa gagtcttgtg aggtgttcaa
    52801 tagcagtcaa cacatacgtg accatgtttt tacttgacaa gtgctagtag aaataataga
    52861 atagtaaaaa acctttaaag cagtccagag aggcagctaa ggttagacgg tgaaagggtg
    52921 gagactaccc atttttcgtg gaaccttgct gttggcaggt tccttttttc gtggcttctg
    52981 ttggccagac tctctcacta gtaaaggtaa aaggagaaac ctatgcgaga acatcgtcca
    53041 gtcattgctc ttgattttcc tagttttgag gcggtcaagg aatttttagc tcttttccca
    53101 gcagaagaaa gcctttatct caaggtaggg atggagcttt attacgcagc ggggcctgag
    53161 attgtgtcct acttaaaagg tttgggtcat agtgtctttt tggatctcaa acttcatgat
    53221 attcctaata cagtcaagtc agccatgaag gtcttgtctc agcttggtgt cgatatgact
    53281 aatgtccatg cggctggtgg tgtagagatg atgaaggcgg cgcgtgaagg ccttgggagt
    53341 caagctaaat tgatcgctgt aactcagctc acatcaacgt cagaagctca gatgcaggag
    53401 tttcaaaata tccaaaccag tctgcaagag tctgtgattc actatgccaa gaagacagct
    53461 gaagctggct tggatggtgt tgtttgctcg gctcaggaag tacaagtcat caagcaggct
    53521 accaatccag attttatctg tctgacacca gggattcgtc cagctggtgc tgcagttgga
    53581 gatcaaaaac gagtcatgac acctgctgat gcctatcaaa tcggcagtga ctatatcgta
    53641 gtgggacgtc ccattaccca agctgaggat cctgttgcag cttatcatgc catcaaggat
    53701 gaatggacac aggactggaa ttaaagaact agattagaaa aataaaagga gaataccatg
    53761 acacttgcta aatatatcgc tagccacctc ttgaaaatcc aagccgttta tctcaaacca
    53821 gaggaaccct tcacttgggc atctggtatc aagtcaccga tttacactga taatcgtgtg
    53881 acactagcct atccagaaac tcgtacccta attgaaaatg gttttgtgga tgctatcaaa
    53941 gaagcctttc ctgaagtaga agtgattgca ggaactgcaa cagcagggat tccacacgga
    54001 gccattattg ctgataagat ggatttgcct tttgcctaca tccgtagcaa accaaaagac
    54061 cacggagctg gtaatcaaat cgaaggtcgc gtagctcaag gtcaaaaaat ggtagtggtt
    54121 gaagacctta tttcaacggg tggttcagtt cttgaagctg tagcagcagc caagcgagaa
    54181 ggagcagatg tgcttggagt tgtagcgatt ttcagctacc aattgccaaa agcagataag
    54241 aactttgcag atgctggtgt taaacttgtg acgctttcaa actatagcga gcttatccat
    54301 ctagcccaag aagaaggtta catcacgcca gagggccttg atcttctaaa acgctttaaa
    54361 gaagaccaag aaaattggca agaaggttag gtcagtaaga taaagagaga cgaggctacc
    54421 gagcctcttt taccatttta tttaaaatat gacaatattt taaataaaat aattgacaac
    54481 gctttctttt gttgatataa tgaaaatgta atatgtatca aattgatcaa tcaactatct
    54541 tgtttatcac ttcttaggag gacgatgtca tgaaaagaag aaaaatatat tccttttttg
    54601 cgtagttaca attgtatcta gtttgtttct ccctacagtt ttagctagac agattggaga
    54661 ttatgattta acgttgccgc gttggggttc ggatacaact agtgagcttg agaaagaaaa
    54721 cgcctctgct ggaattaata ata
//
LOCUS       DAVDWT010000011        47331 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVDWT010000011 DAVDWT010000000
VERSION     DAVDWT010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47331
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.011"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            5..75
                     /locus_tag="VUW50_000769"
     tRNA            5..75
                     /locus_tag="VUW50_000769"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Gly,seq:tcc)
     gene            110..183
                     /locus_tag="VUW50_000770"
     tRNA            110..183
                     /locus_tag="VUW50_000770"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Ile,seq:gat)
     gene            266..355
                     /locus_tag="VUW50_000771"
     tRNA            266..355
                     /locus_tag="VUW50_000771"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:302..304,aa:Ser,seq:tga)
     gene            366..439
                     /locus_tag="VUW50_000772"
     tRNA            366..439
                     /locus_tag="VUW50_000772"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:400..402,aa:Met,seq:cat)
     gene            443..515
                     /locus_tag="VUW50_000773"
     tRNA            443..515
                     /locus_tag="VUW50_000773"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Phe,seq:gaa)
     gene            528..608
                     /locus_tag="VUW50_000774"
     tRNA            528..608
                     /locus_tag="VUW50_000774"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:562..564,aa:Tyr,seq:gta)
     gene            613..683
                     /locus_tag="VUW50_000775"
     tRNA            613..683
                     /locus_tag="VUW50_000775"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:645..647,aa:Trp,seq:cca)
     gene            698..770
                     /locus_tag="VUW50_000776"
     tRNA            698..770
                     /locus_tag="VUW50_000776"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:731..733,aa:His,seq:gtg)
     gene            781..852
                     /locus_tag="VUW50_000777"
     tRNA            781..852
                     /locus_tag="VUW50_000777"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:813..815,aa:Gln,seq:ttg)
     gene            862..945
                     /locus_tag="VUW50_000778"
     tRNA            862..945
                     /locus_tag="VUW50_000778"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:896..898,aa:Leu,seq:caa)
     gene            1052..2329
                     /locus_tag="VUW50_000779"
     CDS             1052..2329
                     /locus_tag="VUW50_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HET7982709.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2405..3889
                     /locus_tag="VUW50_000780"
     CDS             2405..3889
                     /locus_tag="VUW50_000780"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET7982710.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALRERLAA
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGDKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNDAVKTAELMNALNTQGQYELTDFDAEILELFAAEYATEEETAAEIKRVYELDS
                     YIEDSHTAVASAVYRKYQAVTNDATKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLETAPVCHKTTVAAADMQVAVEAYLGL"
     gene            3950..5230
                     /locus_tag="VUW50_000781"
     CDS             3950..5230
                     /locus_tag="VUW50_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET7982711.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMILKG"
     gene            5234..5854
                     /locus_tag="VUW50_000782"
     CDS             5234..5854
                     /locus_tag="VUW50_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7982712.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            6020..7198
                     /locus_tag="VUW50_000783"
     CDS             6020..7198
                     /locus_tag="VUW50_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET7982713.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQETTPAEKQETQVSSQTESAVEATTMP
                     VEEKATETTATSSEAKEVASSNGATAAVSTYQPEETKTISTTYEAPAAPDYAGLAVAK
                     SENAGLQPQTAAFKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPESSELGDKIA
                     EYAIQNMASRGISYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG
                     "
     gene            7712..8050
                     /locus_tag="VUW50_000784"
     CDS             7712..8050
                     /locus_tag="VUW50_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET7982714.1"
                     /translation="MTRYQILLFLLEHSLCNQMAVQERLKIDQAALTRHFKILETEGL
                     VERHRNPENQREVLVEAAKYAKEQLVVNPPLQHIKVKEEMESILTEFERTELSRLLNK
                     LVLGIENIEI"
     gene            8061..8420
                     /locus_tag="VUW50_000785"
     CDS             8061..8420
                     /locus_tag="VUW50_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HET7982715.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKDLLGVFLLYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            8527..9170
                     /gene="pcp"
                     /locus_tag="VUW50_000786"
                     /pseudo
     CDS             8527..9170
                     /gene="pcp"
                     /locus_tag="VUW50_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
     gene            complement(9198..10055)
                     /locus_tag="VUW50_000787"
     CDS             complement(9198..10055)
                     /locus_tag="VUW50_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HET7982716.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10185..11327
                     /gene="tgt"
                     /locus_tag="VUW50_000788"
     CDS             10185..11327
                     /gene="tgt"
                     /locus_tag="VUW50_000788"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET7982717.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11425..13242
                     /locus_tag="VUW50_000789"
     CDS             11425..13242
                     /locus_tag="VUW50_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HET7982718.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNHEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIVGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13395..14546
                     /gene="nagA"
                     /locus_tag="VUW50_000790"
     CDS             13395..14546
                     /gene="nagA"
                     /locus_tag="VUW50_000790"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET7982719.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14709..15767
                     /locus_tag="VUW50_000791"
     CDS             14709..15767
                     /locus_tag="VUW50_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET7982720.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15918..16283
                     /locus_tag="VUW50_000792"
     CDS             15918..16283
                     /locus_tag="VUW50_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HET7982721.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16359..17300
                     /locus_tag="VUW50_000793"
     CDS             16359..17300
                     /locus_tag="VUW50_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HET7982722.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17248..18264
                     /locus_tag="VUW50_000794"
     CDS             17248..18264
                     /locus_tag="VUW50_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7982723.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
                     LGMIVFVSVGVLLALTFYKRSSKMHVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18266..18592
                     /gene="comGC"
                     /locus_tag="VUW50_000795"
     CDS             18266..18592
                     /gene="comGC"
                     /locus_tag="VUW50_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HET7982724.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLKDDGRITEEQAKAYKEYHDKNGEA
                     NRKVND"
     gene            18561..18989
                     /locus_tag="VUW50_000796"
     CDS             18561..18989
                     /locus_tag="VUW50_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET7982725.1"
                     /translation="MEKQIVKSMIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVE
                     EQIFFMEFEELYRETQKRSVASQQKTSLNSDGQTISNGSQKLPVPKGIQAPSDQSITF
                     DRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18994..19254
                     /locus_tag="VUW50_000797"
     CDS             18994..19254
                     /locus_tag="VUW50_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982726.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19217..19678
                     /locus_tag="VUW50_000798"
     CDS             19217..19678
                     /locus_tag="VUW50_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET7982727.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTNARGRGYQPMVYGLKSVRITEDNQLVRFHFQFQKGLEREFIYRVEKEKS"
     gene            19656..20069
                     /locus_tag="VUW50_000799"
     CDS             19656..20069
                     /locus_tag="VUW50_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET7982728.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKVEQESGEQFFNLGQVSYQNKKTGLVTRVRTDKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            20206..20807
                     /locus_tag="VUW50_000800"
                     /pseudo
     CDS             20206..20807
                     /locus_tag="VUW50_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            20868..21821
                     /locus_tag="VUW50_000801"
     CDS             20868..21821
                     /locus_tag="VUW50_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7982729.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQTLKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGF
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKVWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21872..23062
                     /locus_tag="VUW50_000802"
     CDS             21872..23062
                     /locus_tag="VUW50_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET7982730.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            23211..23582
                     /gene="rnpA"
                     /locus_tag="VUW50_000803"
     CDS             23211..23582
                     /gene="rnpA"
                     /locus_tag="VUW50_000803"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET7982731.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            23557..24381
                     /locus_tag="VUW50_000804"
     CDS             23557..24381
                     /locus_tag="VUW50_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET7982732.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            24400..25386
                     /locus_tag="VUW50_000805"
     CDS             24400..25386
                     /locus_tag="VUW50_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HET7982733.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNCYSRTFYITINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            25437..26060
                     /locus_tag="VUW50_000806"
     CDS             25437..26060
                     /locus_tag="VUW50_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HET7982734.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKVFQANHPEWQLQQTSQWMNQAQRLRPHFWVYLQREGKVTEPMIALRLYGNPSDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            26191..27648
                     /locus_tag="VUW50_000807"
     CDS             26191..27648
                     /locus_tag="VUW50_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET7982735.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLLIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27671..27952
                     /locus_tag="VUW50_000808"
     CDS             27671..27952
                     /locus_tag="VUW50_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET7982736.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            28029..28514
                     /locus_tag="VUW50_000809"
     CDS             28029..28514
                     /locus_tag="VUW50_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7982737.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28531..29196
                     /locus_tag="VUW50_000810"
     CDS             28531..29196
                     /locus_tag="VUW50_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HET7982738.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            29200..30063
                     /locus_tag="VUW50_000811"
     CDS             29200..30063
                     /locus_tag="VUW50_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HET7982739.1"
                     /translation="MVRPIGIYEKATPTHCTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAVQEAQAF
                     LYPLIKKAGLM"
     gene            30065..30769
                     /locus_tag="VUW50_000812"
     CDS             30065..30769
                     /locus_tag="VUW50_000812"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HET7982740.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30948..32621
                     /locus_tag="VUW50_000813"
     CDS             30948..32621
                     /locus_tag="VUW50_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET7982741.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVTLQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLATRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32733..33824
                     /gene="ulaG"
                     /locus_tag="VUW50_000814"
     CDS             32733..33824
                     /gene="ulaG"
                     /locus_tag="VUW50_000814"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HET7982742.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33938..35914
                     /gene="tkt"
                     /locus_tag="VUW50_000815"
     CDS             33938..35914
                     /gene="tkt"
                     /locus_tag="VUW50_000815"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7982743.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            36031..36330
                     /gene="yajC"
                     /locus_tag="VUW50_000816"
     CDS             36031..36330
                     /gene="yajC"
                     /locus_tag="VUW50_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET7982744.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            36377..36805
                     /locus_tag="VUW50_000817"
     CDS             36377..36805
                     /locus_tag="VUW50_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HET7982745.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36807..37217
                     /locus_tag="VUW50_000818"
     CDS             36807..37217
                     /locus_tag="VUW50_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982746.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            37517..40168
                     /gene="adhE"
                     /locus_tag="VUW50_000819"
                     /gene_synonym="adhC"
     CDS             37517..40168
                     /gene="adhE"
                     /locus_tag="VUW50_000819"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HET7982747.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFRPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGIVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRKLAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
     gene            complement(40965..41273)
                     /locus_tag="VUW50_000820"
     CDS             complement(40965..41273)
                     /locus_tag="VUW50_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET7982748.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
     gene            complement(41270..41578)
                     /locus_tag="VUW50_000821"
     CDS             complement(41270..41578)
                     /locus_tag="VUW50_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET7982749.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            complement(41583..42878)
                     /locus_tag="VUW50_000822"
     CDS             complement(41583..42878)
                     /locus_tag="VUW50_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET7982750.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            complement(42896..44047)
                     /locus_tag="VUW50_000823"
                     /pseudo
     CDS             complement(42896..44047)
                     /locus_tag="VUW50_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            44237..44431
                     /locus_tag="VUW50_000824"
     CDS             44237..44431
                     /locus_tag="VUW50_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7982751.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSGVTLITGKNGTGKSTLL"
     gene            complement(44797..46107)
                     /locus_tag="VUW50_000825"
     CDS             complement(44797..46107)
                     /locus_tag="VUW50_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET7982752.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNFAITNGTLDVLAQHILYRTRTYPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEDQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTSIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            46344..47216
                     /locus_tag="VUW50_000826"
     CDS             46344..47216
                     /locus_tag="VUW50_000826"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET7982753.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT        14366 a         8450 c        10481 g        14034 t
ORIGIN      
        1 tttagcgggt gtagtttagt ggtaaaacta cagccttcca agctgttgtc gcgagttcga
       61 ttctcgtcac ccgctttgaa ctttgttctt tgtaccaagt ttttgacttg ggcgcgtagc
      121 tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt ccactcgtgc
      181 ccatagtgtt tagtccatta ctaggggatt ggaatattat ctgttcacta agaggacacg
      241 ggcttgttcc cgtataaact attttggagg attacccaag tccggctgaa gggaacggtc
      301 ttgaaaaccg tcaggcgtgt aaaagcgtgc gtgggttcga atcccacatc ctccttttat
      361 attaacgcgg gatggagcag ctcggtagct cgtcgggctc ataacccgaa ggtcgtaggt
      421 tcaaatcctg ctcccgcaat aaggctcggt agctcagttg gtagagcaat ggattgaagc
      481 tccatgtgtc ggcggttcga ttccgtctcg cgccatttat atattttgga agggtagcga
      541 agaggctaaa cgcggcggac tgtaaatccg ctccttcggg ttcgggggtt cgaatccctc
      601 cccttccatt ttacgggcat agtttaaagg tagaactaag gtctccaaaa ccttcagtgt
      661 gggttcaatt cctactgccc gtgttaatag aattatggcg ggtgtggtga agtggttaac
      721 acaccagatt gtggctctgg catgcgtggg ttcgatcccc atcactcgcc tattttatat
      781 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
      841 cagctacccc agttactatt tgccggcgtg gcggaattgg cagacgcgct ggactcaaaa
      901 tccagtgtcc gcaaggacgt gccggttcga ccccggccgc cggtatagta tagtgttagg
      961 aacgttgtta ttcttcgttc cttttttata ttatttttgg tataattata gttattcaaa
     1021 ttttgtttag attgagaaag tgtagggtag tatgtcttgt tctatcgatt tattaaaaca
     1081 tcggtatttg aaaaatatta aagaaaatcc tgaattgttt gtcggaattg agttggagta
     1141 tcctgttgca agtttagaag gggatgctac agatgttgaa gttataaagg atctatttca
     1201 ttatttagtt tctactttgg atctcaccgt agcaaaggta gatgattttg gcaatctgat
     1261 ccagttagta gatccgataa gtcaggatgc tattttattt gaagtttcct atacaacgat
     1321 tgagtttgca tttggtaagg ctgaaacgat tcaagaggtc gaaaatcgtt tcaataatta
     1381 tatgaatgta attcagagaa agttagctga atcaaatcat gctattgttg gctgtggtat
     1441 ccatcccaac tgggataaaa atgagaattg tccagtggct tatccacgct atcagatgtt
     1501 gatggattat ttgaatttga gtagaaatat tattaaatca gatttacatc atttccctga
     1561 atatggtact tttatctgtg ggagccaggt tcagctggat atttcaaaaa ccaactactt
     1621 acgggtgatt aatgctttta ctcaaattga agcggctaag gcttatttat ttgcaaactc
     1681 tgaattttcg ggtgcggatt gggatacgaa aatttcaagg gatattttct gggaagaatc
     1741 tatgcatggt atctatccag agaatgttgg ggtcaatgct agactcctta atgatgaagc
     1801 tgattttttt gactatctaa atcattctgc gatttttact gcggaacgtg atgggcagac
     1861 ctattatttt tatcctattc aggctgggga ctatttggct acgcctgaaa tccaagcatt
     1921 tgctctgaat ggggatgagg ttattattta cccccaagag aaggattttg aaactcatcg
     1981 tagttaccag taccaagact taacgactcg aggaacagtt gagtttcgta gtgtgtgtac
     2041 acagccactt gataggactt ttgcttctgc agcttttcac ttgggattat tggttaattt
     2101 agacaagtta gaagcttact tagaaacagc acctttcttt aaagtatttg gttatgatta
     2161 caagtcttta aggagacaat tttctaagaa aaatcttaca gatgaggaag aaactacgat
     2221 tattgaattt tccaaagact tactcctact agctgaggag ggactagtgg tgagaaataa
     2281 ggaagaaatg acctatttac agcctttgag agaagaattg agcctataat ttctcttata
     2341 aagggagaat tttctgaaaa atcatgatat aatggacgag actatagata aaggatagag
     2401 agtaatgaca ttagtttatc aatcaacgcg tgatgccaac aatacagtaa ctgccagcca
     2461 agcaattttg caaggtttgg cgacggacgg cggtttgttt acaccggtta cttatccaaa
     2521 ggtagatttg gactttgaca aattgaaaga tgcttcttac caggaagttg ctaagctagt
     2581 tttgtcagca tttttagatg actttacagc tgaggagttg gactactgta tcaacaatgc
     2641 ctacgatagc aaatttgata ctccagctat tgcaccatta gtgaaattag atgggcaata
     2701 caatttggaa cttttccatg gttcaacgat tgcctttaag gatatggcct tgtctatttt
     2761 gccatacttt atgacgactg ctgctaagaa acatggtttg gagaacaaga ttgttatctt
     2821 gacagcgaca tctggtgaca cggggaaagc tgctatggcg gggtttgcgg atgtgcctgg
     2881 tactgagatt atcgtctttt atccaaaggg tggtgtcagc aaggtacaag agttgcaaat
     2941 gaccactcag aatggtgaca atactcatgt tatcgctata gatggtaact ttgacgatgc
     3001 gcaaaccaac gtgaagcaca tgtttaacga cgtggctctt cgtgaaagat tagcggccaa
     3061 caagttgcaa ttttcatctg ctaactctat gaatatcggt cgtttggtgc cacagattgt
     3121 ttattatgtt tatgcctatg ctcagttggt caagactggt gagatcgtgg ctggtgataa
     3181 gattaacttc acagtaccaa caggaaactt tggaaatatc ttggctgcct tttatgccaa
     3241 acaaatcggt ctcccagttg gtaaattgat ctgtgcttca aatgacaaca atgttttgac
     3301 agacttcttc aagacacgtg tttacgacaa gaaacgtgag tttaaggtaa caaccagccc
     3361 atctatggat atcttggtat cttcaaactt ggagcgtttg attttccatc ttttggggaa
     3421 tgatgcggtt aagacggctg aactcatgaa tgccttgaat acacaaggac aatatgaact
     3481 gactgatttt gatgcagaga ttctggagct ctttgcagct gaatatgcga ctgaggaaga
     3541 aacagcggca gaaatcaagc gtgtttatga gttagattct tatattgagg attcacatac
     3601 ggcggttgcc tcagcagttt atagaaaata ccaagcggtt actaatgatg cgactaagac
     3661 agtgattgct tcaacagcta gtccctacaa gttcccagta gttgcagtag aagctgtaac
     3721 tggaaaagca ggtttaacag actttgaagc tttggctcaa ttacatgaaa tctcaggcgt
     3781 tgcagtgcca ccagcagttg atgggcttga gacagctcct gtttgtcaca agacaacagt
     3841 ggcagctgct gacatgcaag tagcggttga ggcttattta ggactttaag atagagggag
     3901 taaactcggt tgggaaacca actgagtttc ttttcatcag gaggagagat tgtttaagaa
     3961 aaataaagac attcttaata ttgcattgcc agctatgggt gaaaactttt tgcagatgct
     4021 aatgggaatg gtggacagtt atttggttgc tcatttagga ttgatagcta tttcaggggt
     4081 ttcggtagct ggtaatatta tcaccattta tcaggcgatt ttcatcgctc tgggagctgc
     4141 tatttccagt gttatttcaa aaagcatagg gcagaaagac cagtcgaagt tggcctatca
     4201 tgtgactgag gcgttgaaga ttaccttact attaagtttc cttttaggat ttttgtccat
     4261 cttcgctggg aaagagatga taggactttt ggggacggag agggatgtag ctgagagtgg
     4321 tggactgtat ctatctttgg taggcggatc gattgttctc ttaggtttaa tgactagtct
     4381 gggagccttg attcgtgcaa cgcataatcc acgtctgcct ctctatgtta gttttttatc
     4441 caatgccttg aatattcttt tttcaagtct agctattttt gttctggata tggggatagc
     4501 tggtgttgct tgggggacaa ttgtgtctcg tttggttggt cttgtgattt tgtggtcaca
     4561 attaaaactg ccttatggga agccaacttt tggtttagat aaggaactgt tgaccttggc
     4621 tttaccagca gctggagagc gacttatgat gagggctgga gatgtagtga tcattgcctt
     4681 ggtcgtttct tttgggacgg aggcagttgc tgggaatgca atcggagaag tcttgaccca
     4741 gtttaactat atgcctgcct ttggcgtcgc tacggcaacg gtcatgctgt tggcccgagc
     4801 agttggagag gatgattgga aaagagttgc tagtttgagt aaacaaacct tttggctttc
     4861 tctgttcctc atgttgcccc tgtcctttag tatatatgtc ttgggtgtac cattaactca
     4921 tctctatacg actgattctc tagcggtgga ggctagtgtt ctagtgacac tgttttcact
     4981 acttgggacc cctatgacga caggaacagt catctatacg gcagtctggc agggattagg
     5041 aaatgcacgc ctcccttttt atgcgacaag tataggcatg tggtgtatcc gcattggaac
     5101 aggatatctg atggggattg tgcttggttg gggcttacct ggtatttggg cagggtctct
     5161 cttggataat ggttttcgct ggttatttct acgctatcgt taccagcgct atatgatctt
     5221 gaaaggatag gaaatgcaaa aaacagcttt tatttgggat ttagacggga ctttattgga
     5281 ctcttacgaa gcgattttat cagggattga ggagactttt gctcagtttt ctattcctta
     5341 tgataaggag aaggtgagag agtttatctt caagtattcg gtgcaagatt tgcttgtgcg
     5401 ggtggcagaa gatagaaatc tggatgttga ggtgctaaat caggtgcgtg cccagagtct
     5461 ggctgagaag aatgctcagg tagttttgat gccaggtgcg cgtgaggtgc tagcttgggc
     5521 agacgaatca ggaattcagc agtttatata tactcataag gggaacaacg cttttaccat
     5581 tctcaaggac ttgggggtgg aatcctattt tacagagatt ttaaccagtc agagtggctt
     5641 tgtgcggaag ccaagtccag aagcggctac ctatctgcta gataagtatc agttgaattc
     5701 tgataatact tattatatag gggatcggac tctggatgtg gaatttgccc agaatagtgg
     5761 gattcaaagt atcaactttt tagagtctac ttatgaaggg aatcacagga ttcaagcgtt
     5821 agcagatatt tcccgtattt ttgagactaa gtgataaaaa gattgtgtca gttttgtgac
     5881 agagacctaa caaactattt caagtaacct agtttgttac aaggaataga cagttctgtt
     5941 aaataggccc gagagggctt tttttctaca ttttttgtgt tatgatagac aggtactcat
     6001 ttgaaaggaa tttgaaagaa tgaagaaaag aatgttatta gcgtcaacag tagccttgtc
     6061 atttgcccca gtattggcaa ctcaagcaga agaagttctt tggactgcac gtagtgttga
     6121 gcaaatccaa aacgatttga ctaaaacgga caacaaaaca agttataccg tacagtatgg
     6181 tgatactttg agcaccattg cagaagcctt gggtgtagat gtcacagtgc ttgcgaatct
     6241 gaacaaaatc actaatatgg acttgatttt cccagaaact gttttgacaa cgactgtcaa
     6301 tgaagcagaa gaagtaacag aagttgaaat ccaaacacct caagcagact ctagtgaaga
     6361 agtgacaact gcgacagcag atttgaccac taatcaagtg accgttgatg atcaaactgt
     6421 tcaggttgca gacctttctc aaccgattgc agaagctcca aaagaagtag catcaagttc
     6481 agaagttaca aagacagtga ttgcttctga agaagtggca ccatctacgg gcacttctgt
     6541 cccagaggag caaacggccg aaacaagcag tgcagttgca gaagaagctc ctcaggaaac
     6601 gactccagct gagaagcagg aaacacaagt gagctctcaa accgagtcag cagtggaagc
     6661 aactacaatg ccagtagaag aaaaagcaac cgaaacaact gcaacaagtt cagaagcaaa
     6721 agaagtagca tcatcaaatg gagctacagc agcagtttct acttatcaac cagaagagac
     6781 gaaaacaatt tcaacaactt acgaggctcc agctgcgccc gattatgctg gactggcagt
     6841 agcgaaatct gaaaatgcag gtcttcaacc acaaacagct gcctttaaag aagaaattgc
     6901 taacttgttt ggcattacat cctttagtgg ttatcgtcca ggagacagtg gagatcacgg
     6961 aaaaggtttg gctatcgact ttatggtacc agaaagttca gaattagggg ataagattgc
     7021 ggaatatgct attcaaaata tggccagccg tggcattagt tacatcatct ggaaacaacg
     7081 tttctatgct ccattcgata gcaaatatgg gccagctaac acttggaacc caatgccaga
     7141 ccgtggtagt gtgacagaaa atcactatga tcacgttcac gtttcaatga atggataaac
     7201 ccgacttgat aacatcattt tgacgaatga gatctagctt tcgtgatgga aagcgattct
     7261 cgttcgtttt ttctttgtca tactcttcga aaatctcttc aaaccacgtc agttttatct
     7321 gaaacctcaa agctgtgctt tgagcaacct gcgactagct tcctagtttg ctttttgatt
     7381 ttcattgagt atcaatttga atggaaaatg gaaagttatc atcttgtaat gagttaagca
     7441 acattcttgc aatctatttt atagtagatg gaaactagaa tagtacacaa ctgcttctaa
     7501 aacattgtta gaaatcgatt tgactgtcct gatcgatttg tcctgctctt atttcatttc
     7561 actatacttt atattataat tgactggtca actattgata aatcaataaa aagagagggg
     7621 aagaaatgct agagattcaa gatttactgt atcaactccg cttgtctgag caagcgagta
     7681 cgcaattgtt tgaaaaaagg cttggattag tttgacacgg tatcagattt tactgttttt
     7741 gctggagcat tctctttgta accaaatggc ggttcaggag cgtttgaaaa ttgatcaggc
     7801 tgctttgaca cggcatttca agattttgga aacggaaggt ttggtggagc gtcatcgtaa
     7861 tcctgaaaat cagcgggaag tgttggtaga ggctgcgaag tatgccaagg agcagttagt
     7921 ggtgaatccc cctctgcaac atatcaaggt taaggaagag atggaaagta tcttaacaga
     7981 gtttgagaga acagaactca gccgtttatt aaataaattg gttttgggta ttgaaaatat
     8041 agaaatttaa ggagaaatag atgtcaatta ttttaacaac gatcgttgct ttggagcatt
     8101 tttacatttt ttatttggaa agtattgcca cgcaatcaga tgcgactagt cgtgtattta
     8161 atatggaaaa ggaagaattg gctcatccgt cagtaagttc attgttcaaa aatcaaggaa
     8221 tttataagga tctgctagga gtctttctct tgtatggcat ttatttctca cagaatttag
     8281 aaattgtgac tatttttgtc ttatttgtga ttggtgctgc gacttacggc tctttaacag
     8341 cggataaaaa aattattttg aaacaaggtg gatcagctat tttggccttg attagtattt
     8401 tactctttaa atacacttga aggtcgattc taatctcgct aatccttttt aatccagaat
     8461 aagggaaata tgttatactt gtttttaaga aaaaagtctc attgaattgg ttttgaggag
     8521 ttagaaatga aagtattagt gacaggtttt gagccctttg gaggggaaaa gggcaatcca
     8581 gctttggagg ccattaaagg tttaccagct gaaatccatg gtgctgaggt ccgttggcta
     8641 gaggtgccga cagttcttca caaatctgct caagtattgg aagaagagat gaatcgttat
     8701 caacctgact ttgtcctttg tattgggcaa gctggtggaa gaactagttt gacacctgaa
     8761 cgagtggcca ttaatcaaga cgatgcacgc atttctgata acgaagataa tcaaccgatt
     8821 gaccgtccca ttcgcccaga tggtgcttcg gcctacttta gtagtttgcc gattaaagcg
     8881 atggttcaag ctataaaaaa gaaggattac cggcctctgt ttccaatacg gcagggactt
     8941 ttgtctgcag ccatttgatg tatcaggctc tctatttggt agaaaagaaa tttccatatg
     9001 ttaaggcagg ttttatgcat attccttata tgatggaaca ggtggtgaac agaccgacta
     9061 ctccaactat gagtttagtg gatattcggc gagggataga agcagcaatc ggcgctatga
     9121 tagaacatgg agatcaggaa ctcaagttgg taggcggaga aactcattga tagaaaaaag
     9181 cttgagggga aaaaccttca agcttttgga cgttttcgag ccaatactgc tcggtaaaac
     9241 ataattttag tgcattggat ataaggtagg agtgaaaaac tagcaatgcc aaaggtaatc
     9301 caattgagga agtaccaagg aagaagctgt aaatctagga caaagtgctg gaacttgtag
     9361 cccttcataa aggaacggct agtttttagg attcgtcttg gtgggacctg tcctaggtct
     9421 agactataac agagaagaaa ttccacctgt gaataggcat aatactgtgg aatatagagg
     9481 atattcccta caatgatcaa gatgagactt gcaagaaagt agagtccaaa gaccatgagg
     9541 aaacgctcgg tttcaactga tgagagatct agatttggaa actcaggatg tagggtgacg
     9601 aattttttgg ctaaaaagct actataaaag aggaggtaaa tcccaagtaa attagggata
     9661 ctccataaaa agagatagaa acgtttgaga agtagggtca aaaaggtttg agaaaagcgc
     9721 tcctcatcaa agagagctag gctgtttttt acagatggct ccgttttaga atctttcatg
     9781 agtgtcagtg ttgcatagac ggaactggtc aaaagaatag tcccgataaa ggagactagt
     9841 agaggaaaga ggtaggcttg aagtatttgg ccaagtatgc tgaaaaatgg ctgttctaaa
     9901 acagtcccgt ggatccgaga taagggatta agaaaactag ataagatgac cagcatactg
     9961 ggaaggatat agaggagaaa gagacggggg gtgtcagcct gaaaatgttt tgactcctga
    10021 cgaattgttt ttaaatcaat ttttggatag ttcattctct tattatacca tagttcttat
    10081 acatagttcg tgacagttcc tacttttttt gataaaatca tacagtgtgt ccttgggcac
    10141 actgtatgaa ctgggactgt ctttcccagc ttcggaggta aaaaatgtca gattcaccaa
    10201 tcaaatatcg tttgattaag aaagaaaaac acacaggagc tcgtctagga gaaatcatca
    10261 ctccccacgg tacctttccg acacctatgt ttatgccagt tgggacacaa gccactgtca
    10321 aaactcagtc acctgaagaa ttgaaggaga tgggttcggg aattatccta tcaaacacct
    10381 atcatctctg gcttcgccct ggagatgaac tcattgcacg cgctggtggt ctccacaagt
    10441 tcatgaattg ggaccagcct atcttgacag atagtggtgg ttttcaggtt tattctttag
    10501 cagatagccg taatatcaca gaagaaggag taacctttaa aaatcatcta aatggttcta
    10561 agatgttcct atccccagaa aaagccatct ctattcagaa taatctgggt tcagacatca
    10621 tgatgtcctt tgatgaatgt cctcagtttt atcaacctta tgactacgtt aagaaatcga
    10681 tcgagcgtac cagccgttgg gctgagcgtg gtttgaaggc tcaccgtcgt ccacatgacc
    10741 aaggtttgtt tggaattgtg caaggtgcag gatttgaaga ccttcgccgc caatcagctc
    10801 atgatcttgt cagcatggat ttctcaggct actctatcgg tggtttggca gtgggagaaa
    10861 cccatgaaga gatgaatgcg gtcttggact ttacaactca actgctacct gaaaataaac
    10921 ctcgttatct gatgggtgtg ggagcgccag atagcttgat cgatggggtc attcgtgggg
    10981 tggatatgtt tgactgtgtc ttaccgactc gaattgctcg taacgggact tgtatgacca
    11041 gtcaaggacg tttggttgtg aaaaatgccc agtttgctga ggactttacg ccactggatc
    11101 ctgagtgtga ctgctacaca tgtaataact atacacgcgc ttaccttcgt cacctgctca
    11161 aggctgatga aacctttggt atccgcttga ctagctacca caatctttac ttcttgctta
    11221 acctgatgaa gcaagtgcga caagccatca tggatgacaa tctcttggaa ttccgtgagt
    11281 attttgtgga aaaatatggc tataataagt caggacgtaa tttctaaaat ggaattgata
    11341 taaaaaaatc ctaagttttc tcttaggatt tttcttcttt ttttgataga ataaagtgta
    11401 caatgaaagg aagaataaac tcgtatgcgc attaaatggt tttccttgat taggattata
    11461 ggtttacttt tggtactctt gtatcacttc tttcagacga tctttcctgg aggatttttc
    11521 ggggtagatg tctttttcac attttcaggt ttcctgatta cagctctact cattgaagaa
    11581 ttttctaaaa accatgagat tgatttgata ggatttttta gaagacgctt ttatcggatt
    11641 gtgccacctg tggttttgat ggtcttggtg accatgcctt ttactttctt ggttcgccaa
    11701 gactatgttg ctggaattgg tggccagatt gcgggcgtct taggctttat gaccaacttc
    11761 tatgaactcc taacaggtgg gagttatgaa tctcagttca ttcctcattt gtttgttcat
    11821 aattggagtt tggcagttga ggttcactac tatattcttt ggggattggc agtttggttc
    11881 ttatccaaac aagctaaatc aaatggtcag ttgaagggga tggtctttct cttatctgct
    11941 gttgccttct tgatcagttt cttctccatg tttattggta gttttctagt gacctcttat
    12001 tcctctgttt atttctccag tttaactcat gtctatccat tctttttggg aagtatgtta
    12061 gcaactattg taggcgttcg tcagacgact tccctcgtca agcagttgga taaaatctgg
    12121 gatttacgaa agactttggt agtttttgga ggaggctttg gtttcctagt tcttttgact
    12181 ttctttgtca aattcactta tctttttgcc tatcttatcg gcttcttgct tgccagtctt
    12241 gcagctcttg ccatgattct ggcggcgcgt gtcttacatg aaaagacaca tcatatacag
    12301 gagccgaaga ttatcagctt tctagcggat actagctatg cggtttatct tttccattgg
    12361 cctttctata tcattttctc acagttgaca tcaaatcttc ttgctgtatt actgactctg
    12421 atttgttctt atggctttgc cagtctgtca ttctatgtat tggaaccttg gattgtaggc
    12481 aagaacacac ctattttaca aactcttcgt cccctgcctt atattcacgc aattcttgca
    12541 gcaggtacag gaatcttgac cttcattgtc tgcatggtga ccttgttggc accacaagtg
    12601 ggagcgtttg agacagactt gactgtcaat ggcttgaagc aagctgcaac aaatattggc
    12661 cagaccaagg tgatggcaga acgggcagat gcaaacagtt tgggaattgc tgatggcact
    12721 atgttaattg gtgactcagt ggctttaagg gcaaatacag cactacagac agctcttcct
    12781 ggagcacaga ttaacgcgca ggtcagcgta acaaccaaga ccgcaaatga aatcatgcta
    12841 aataatagcc agaataaatt tttacctaag acggtggtca ttgcgactgg ggtaaataat
    12901 cctgagaatt acaaggatga ctgggacagt atcgtgaaaa atcttcctaa gggacaccat
    12961 atgattttgg tgactcctta tgagggagat aagacaaaag agacctatgc catcgttgag
    13021 aaggctgctg cctatatgag agaattggca gagaagacac cttacattac gatagcagat
    13081 tggaatcaag ttgcgaaaga gcatccagaa atttgggcag gaacagacca ggttcatttc
    13141 gggagtgaga gtagcactat cgaagcagga gcaaaattgt atgcagatac gattgccaca
    13201 gctttgcaga cagctcaaga caagccggtt aaatcaaaat aacttgtatt aaaaaagaga
    13261 agagttggag aaatccagct cttttaaaat atctccaaaa aataggtcta taccatttac
    13321 aaataaaaaa gaaaggttta taatgtaatt gacataataa attgtagaat caatctttta
    13381 aggaggttaa cattatgcct aactatatta aagcggatca gtttttctac ccacacggag
    13441 ttcgtcgagg tggttacttg gaacttgtgg acggcaagtt tgggaaacat gtagagcaga
    13501 ttcctgaagg ggctgaggtg attgactata caggttatag cattgcccca ggacttgtgg
    13561 atactcatat tcatggatat gcaggtgtag atgtgatgga caacaacatt gaaggtacat
    13621 tgcatactat gagtgaagga cttcttagta ccggtgttac cagtttctta cccacaactt
    13681 taacagccac ttatgagcaa ttgcttgcag tcactgaaaa tcttggaaac cattataaag
    13741 aagcaacagg tgctaagatt cgtgggattt attatgaagg tccatatttc acagaaactt
    13801 ttaagggggc acaaaatcca acttatatga gagacccggg tgttgaggag tttcattctt
    13861 ggcaaaaagc ggcaaatggc ttgcttaata aaattgccct tgcaccagaa cgtgatgggg
    13921 tggaagactt tgttcgtaca gttacgggcg aaggtgtgac ggttgctctt ggacattcaa
    13981 acgcgacttt tgatgaagcc aaaaaagcag tcgatgctgg agcgagtgtt tgggtgcatg
    14041 cctacaatgg aatgcgtggg ttgactcacc gtgaattggg tatggttgga gccatgtacc
    14101 aattgccaca tacctatgca gagttgatct gtgatggtca ccacgtagat ccaaaggctt
    14161 gcgaaattct tatcaaacaa aaaggaacag aaaacatcgc tcttatcaca gactgtatga
    14221 cagctggggg attggaagac ggagattata tgttgggaga attcccagtt gtcgttgcaa
    14281 atggaactgc acgcctcaaa tcgacaggta acttggcagg ttctatcctc aaactcaaag
    14341 atggtttgaa gaatgtggtc gaatggggaa ttgcgaatcc gcatgaagca gtcatgatgg
    14401 ccagcttcaa cccagctaaa tccgttcaca tcgatgacgt ctgtggccaa atccgtgaag
    14461 gctacgacgc tgacttcatc gtattagata aagatttgga attggtagca acctacctag
    14521 atggtgtgaa acgttatcaa gcataagaga gaaggcagaa gttagagact agcttctgct
    14581 tttttataca gggtacttta ctggtaaata aaaagttaaa aaaatcacaa aaaaagcttg
    14641 aagaaacaaa gaaaaaggct tacaattgaa ctataaatat tacaaataaa aaaacggagg
    14701 agtgctttat gaaagcctat acttatgtta aaccaggact tgcttctttt gttgatgtag
    14761 acaaaccagt tattcgcaag ccaacagacg ctattgtgcg tattgtaaaa accactattt
    14821 gtggaacaga cctccatatt atcaaagggg atgttcctac ttgccaaagt ggtaccattc
    14881 ttggccacga agggattggg attgttgaag aagttgggga aggagtttcc aacttcaaaa
    14941 aaggtgacaa ggtcttgatt tcttgcgtct gtgcctgtgg taaatgttac tactgtaaaa
    15001 aaggaattta tgctcactgt gaagacgaag ggggctggat tttcggtcac ttgattgatg
    15061 gtatgcaggc tgaatatcta cgtgtccctc atgcagataa tactctttac catactccag
    15121 aagacttgtc agatgaagct ttggttatgc tgtcagacat tctgcctact ggatatgaaa
    15181 ttggtgtctt aaaagggaaa gtagaacctg gttgcagcgt agccattatt ggttcaggtc
    15241 cagttggatt ggctgctctt ttaacagccc aattctattc accagctaaa ttgattatgg
    15301 tagacctaga cgataaccgc ttggaaactg ccctatcatt cggtgcgact cataaggtta
    15361 attcttcaga ccctgaaaaa gccattaaag aaatttatga tttgacagat ggtcgtggtg
    15421 tggatgtcgc tatcgaagct gttggtattc ctgcaacatt tgatttctgt caaaagatta
    15481 tcggtgtaga cggaacggtt gccaactgtg gtgtgcatgg taaaccagtt gaattcgatt
    15541 tagataaact ttggattcgc aacatcaatg taacaactgg tttggtatct acaaatacga
    15601 ctccacaatt gttgaaagca cttgaaagtc ataagattga accggaaaaa ttggtaactc
    15661 actatttcaa actcagtgaa attgaaaaag cctacgaagt cttcagtaag gcagcagacc
    15721 accatgccat taaggtcatt atcgaaaacg atatctcaga agcctaagta gtaaaaatat
    15781 ttttgtacat aagcaaatag aaattcagtc atccatcaga tggctggatt ttttatcaaa
    15841 aaattaagaa atgagcatat ttctttcctt gtctggcgga attggttata atatacggta
    15901 caaaggaatg aatgaatatg tatcgtgtta tagaaatgta cggagatttt gaaccgtggt
    15961 ggttcttaga aggttgggaa gaagatattg tagcaagtag aaaatttgac cagtattatg
    16021 atgctctcaa atactacaaa acttgctggt ttagattgga acaagaatcc cctctttata
    16081 aaagtagaag cgacttgatg accatttttt gggacccgga agaccaacgc tggtgtgatg
    16141 aatgtgatga gtatttacaa caataccatt ctttggctct tttgcaggat gagcaggtta
    16201 tcccagacga aaaactacgc tcaggctatg aaaaacaaac cagtcaggaa aggaatcgtt
    16261 cttgccgtat gaaattaaaa tagagaaaag taactttttt ggagttgctt tttttatttt
    16321 tctaacgctt tgcgaatagt ataggtgagg aggtaagtat ggttcaagaa attgcacaag
    16381 aaatcattcg ttcagctcgg aaaaaaggga cgcaggatat ttattttgtt cctaagttag
    16441 atgcctatga gcttcatatg agggtaggag acgagcgctg taaaattggt agctatgatt
    16501 ttgaaaagtt tgcagccgtt atcagtcact ttaagtttgt ggcgggtatg aatgtgggag
    16561 aaaaaagacg tagtcaactg ggttcctgtg attatgccta tgaccagaag atggcgtctc
    16621 tacgtttatc tactgtaggc gattatcggg ggcatgagag tttggttatc cgtttgttgc
    16681 acgatgagga acaggacttg catttttggt ttcaggatat tgaagaatta ggcaagcagt
    16741 acaggcaacg gggactctat ctttttgctg gtccggttgg gagtggtaag acgaccttga
    16801 tgcatgaatt gtccaagtca ctctttaaag gacagcaagt tatgtccatc gaagatcctg
    16861 tcgaaatcaa gcaggacgac atgcttcagt tgcagttgaa cgaagcaatc ggcctaacct
    16921 atgaaaatct aatcaaactt tccttgcgtc atcgaccaga tctcttgatt atcggagaaa
    16981 ttcgtgacag cgagacggcg cgtgcagtgg tcagagctag tttgacaggt gcgacagtct
    17041 tttcaaccat tcacgccaag agtatccgag gtgtttatga gcgtctgttg gagttgggtg
    17101 tgagtgaaga agaattggca gttgttctgc aaggagtctg ctaccagaga ttaatcgggg
    17161 gaggaggaat cgttgacttt gcaagcagag attatcaaga acaccaagca gccaagtgga
    17221 atgagcaaat tgaccagctt cttaaagatg gacatatcac aagtcttcag gctgagacgg
    17281 aaaaaattag ctacagctaa gcaaaaaaat atcatcaccc tatttaacaa tctcttttct
    17341 agcggttttc atctggtgga gactatctcc tttttagata ggagtgcttt gttggacaag
    17401 cagtgtgtga cccagatgcg tgtgggcttg tctcagggga aatcattctc agaaatgatg
    17461 gaaagtttgg gatgttcaag tgctattgtc actcagttat ccctagctga agttcatggc
    17521 aatctccacc tgagtttggg aaagatagaa gaatatctgg acaatctggc caaggtcaag
    17581 aaaaaattga ttgaagtagc gacctatccc ttgattttgc tgggttttct tctcttaatt
    17641 atgctggggc tacggaatta cctgctccca caactggata gtagcaatat tgccacccaa
    17701 attatcggta atctgccaca aatctttcta ggaatgatag tttttgtttc agtaggtgtc
    17761 cttttagcac tcacttttta taaaagaagt tctaagatgc acgtcttttc tatcttagcg
    17821 cgccttcctt ttattggaat ctttgtgcag acctatttga cagcctatta tgcacgtgaa
    17881 tgggggaata tgatttcaca gggaatggag ttgacgcaga tttttcaaat gatgcaggaa
    17941 caaggttccc agctctttaa agaaatcggt caagatctgg ctcaaaccct gaaaaatggc
    18001 cgtgaatttt ctcagacgat aggaacctat cctttcttta ggaaggaatt gagtctcatc
    18061 atagagtatg gggaagttaa gtccaagctg ggtagtgagt tggaaatcta tgctgaaaaa
    18121 acttgggaag ccttttttac ccgagtcaac cgcaccatga atttggtgca gccactggtt
    18181 tttatctttg tggcactgat tatcgtttta ctttatgcgg caatgctcat gcccatgtat
    18241 caaaatatgg aggtaaattt ttaaaatgaa aaaaatgatg acattcttga aaaaagctaa
    18301 ggttaaagct tttacattgg tggagatgtt ggtggtcttg ctgattatca gcgtgctttt
    18361 cttgctcttt gtacctaatc tgaccaagca aaaagaagca gtcaatgaca aaggaaaagc
    18421 agctgttgtt aaggtggtgg aaagccaggc agaactttat agcttggaaa agaatgaaga
    18481 tgctagccta agcaagttaa aagacgatgg gcgaatcacg gaagaacagg ctaaagctta
    18541 taaagaatac catgataaaa atggagaagc aaatcgtaaa gtcaatgatt aaggccttta
    18601 ccatgctgga aagtctcttg gttttgggtc ttgtgagtat ccttgccttg ggcttgtccg
    18661 gctctgttca gtccactttt gcggcggtag aggaacagat tttctttatg gagtttgaag
    18721 aactctatcg ggaaacccaa aaacgcagtg tagccagtca gcaaaagact agtctgaact
    18781 cagatgggca gacgattagc aatggcagtc aaaagttgcc agtccctaaa ggaattcagg
    18841 ccccatcaga tcaaagtatt acatttgacc gtgctggggg caattcgtcc ctggctaagg
    18901 ttgaatttca gaccagtaaa ggagcgattc gctatcaatt atatctagga aatggaaaaa
    18961 ttaaacgcat taaggaaaca aaaaattagg gcagtgattt tactggaagc agtagtcgct
    19021 ctagctatct ttgccagcat tgcgaccctc cttttgggac aaattcaaaa aaataggcaa
    19081 gaggaagcaa aaatcttgca aaaggaagaa gtcttgaggg tagctaagat ggccctgcag
    19141 acggggcaaa atcaggtaag catcaacgga gttgagattc aggtattttc tagtgaaaaa
    19201 ggattggagg tctaccatgg ttcagaacag ttgttggcaa tcaaagagcc ataaggtcaa
    19261 ggcttttacc ttgttagaat ccctgcttgc cctcattgtc atcagtgggg gattactcct
    19321 ttttcaagct atgagtcagc tcctcatttc agaagttcgc taccagcaac aaagcgagca
    19381 aaaggagtgg ctcttgtttg tggaccaact tgaggtagaa ttagaccgtt cgcagttcga
    19441 aaaagtagaa ggcaatcgcc tatacatgaa gcaagatggc aaggacatcg ccatcggtaa
    19501 gtcaaagtca gatgatttcc gtaaaacgaa tgctcgtggt cgaggttatc agcctatggt
    19561 ttatggactc aaatctgtac ggattacaga ggacaatcaa ctggttcgct ttcatttcca
    19621 gttccaaaaa ggcttagaaa gggagttcat ctatcgtgtg gaaaaagaaa aaagttaagg
    19681 caggtgttct cctctacgca gtcaccatag cagccatctt tagtcttttg ttgcaatttt
    19741 atttgaaccg acaagtcgcc cactatcaag actatgcttt gaataaagaa aaattggttg
    19801 cttttgctat ggctaaacga accaaagata aggttgagca agaaagtggg gaacagtttt
    19861 ttaatctagg tcaggtaagc tatcaaaaca agaaaactgg cttagtgacg agggttcgta
    19921 cggataagag ccaatatgag tttctgtttc cttcagtcaa aatcaaagaa gagaaaagag
    19981 ataaaaagga agaggtagcg accgattcaa gcgaaaaagt ggagaagaaa aaatcagaag
    20041 agaagcctga aaagaaagag aattcatagt caattcaact ataatgcgtt gaatccagaa
    20101 tagtccactg tagtttctag aaaattgctg gaaatggatg ttaagctcca attcatttgt
    20161 ttatatctta tttcagttta ctatactttg tgctaaatta aagatatgaa acatgatttt
    20221 aaccacaaag cagaaacttt cgattcccct aaaaatatct tcctcgcaaa cttggtatgt
    20281 caagcagccg agaaacagat tgatcttcta tcagacaaag aaattttaga tttcggtggt
    20341 ggcacgggtc tattagcctt gcccctaacc cctaacccct agccaagcag gctaagtcag
    20401 tcactcttgt agacatttct gagaaaatgt tggagcaagc tcgtttgaaa gtggagcagc
    20461 aagcaatcaa gaatatccag tttttggagc aagatttacc gaaaaatccc ttggagaaag
    20521 agtttgattg ccttgctgtt agtcgggttc ttcatcatat gcctgatttg gatgcggctc
    20581 tctcactgtt tcatcaacat ttgaaggaag atgggaaact catcattgct gattttacca
    20641 agacagaagc taatcatcat ggatttgatt tagctgaact ggaaaacaag ctaattgagc
    20701 atggtttttc atctgtgcat agtcagattc tctatagtgc tgaagacctg tttcaaggaa
    20761 atcactcaga attcttttta atagtagccc aaaaatcact cgcctagtca gggagtgatt
    20821 tttctataag gatggaaaaa agaagggaaa tttggtaaga taggaatatg gattttgaaa
    20881 aaattgaaca agcttatacc tatttactag agaatgtcca agtcatccaa agtgatttgg
    20941 cgaccaactt ttatgacgcc ttggtggagc aaaatagcat ctatctggat ggtgaaactg
    21001 agttaaacca ggtcaaagac aacaatcaaa cccttaagcg tttagcacta cgcaaagaag
    21061 aatggctcaa gacctaccag tttctcttga tgaaggctgg gcaaacagaa cccttgcagg
    21121 ccaatcacca gtttacaccg gatgctattg ctttgctttt ggtgtttatt gtggaagagt
    21181 tgtttaaaga ggaggaaatt actatcctcg aaatgggttc tgggatggga attctaggcg
    21241 ctactttctt gacctcgctt gataaaaagg tggattactt gggaatggaa gtggatgatt
    21301 tgctgattga tctggcagct agcatggcag atgtaattgg tttgcaggct ggctttgtcc
    21361 aaggagatgc cgttcgccca caaatgctca aagaaagcga tgtggtcatc agtgacttgc
    21421 ctgtcggcta ttatcctgat gatgccgttg cgtcgcgtca tcaagttgct tctagccaag
    21481 aacatactta cgctcatcac ttgctcatgg aacaagggtt taagtacctt aagtcagatg
    21541 gatatgctat ttttctagct ccgagtgatt tgttgaccag tcctcaaagt gatttgttga
    21601 aagtctggtt gaaagaggag gcaagtctgg ttgctatgat tagtttacct gaaaatctct
    21661 ttgctaatgc taaacaatct aagactattt ttatcctaca gaagaaaagt gaaatagcag
    21721 tggagccttt tgtttatcca cttgctagct tgcaagatgc aagtgtttta atgaaattta
    21781 aagaaaattt tcaaaaatgg actcaaggta ctgaaatata aaatagattt tgttataata
    21841 gttgaaaacg cttaaaaagg ggtatcatgt tatgacaaaa acaattgcaa tcaatgcagg
    21901 aagttcaagt ttgaaatggc aattatactt aatgccagaa gaaaaagtat tggcgaaagg
    21961 tttgattgaa cgtatcggtt tgaaagattc aatttcaact gtaaaatttg acggccgttc
    22021 tgaacaacaa attttggata ttgaaaatca tacacaagcc gttaaaattt tattggatga
    22081 cttgattcgt ttcgatatta tcaaggctta tgacgagatt acaggtgttg gacatcgtgt
    22141 tgttgctggt ggagaatatt tcaaagaatc aacagttgtt gagggagatg ttttagaaaa
    22201 agttgaagag ttgagtttgt tggctcctct acacaacccg gccaatgcag caggtgttcg
    22261 tgccttcaag gaattgttgc cagacattac cagtgtagtt gtttttgata cttccttcca
    22321 cacaagtatg ccagagaaag cttatcgcta ccctctacca acaaaatatt acacagaaaa
    22381 caaggttcgt aaatacggtg ctcatggtac aagtcaccag tttgtagcag gagaagctgc
    22441 aaaactcttg ggacgtccat tagaagactt gaagttaatt acctgtcata ttggtaacgg
    22501 aggctcaatt acagctgtga aagccggcaa atctgtagac acttctatgg ggttcactcc
    22561 tcttggtggt attatgatgg gaacgcgtac aggggatatt gatccagcta tcattcctta
    22621 tttaatgcaa tatacagagg attttaacac accagaagat atcagtcgtg ttcttaaccg
    22681 tgaatcaggt cttttgggag tttctgctaa ttctagcgat atgcgcgata tagaagcagc
    22741 tgtagcagaa gggaatcacg aggctagctt ggcttatgaa atgtatgttg accgtatcca
    22801 aaaacatatc ggtcagtacc ttgcagtgct aaatggagca gatgccattg ttttcacagc
    22861 aggtgtcggt gaaaatgcag agagtttccg tcgtgatgta atctcaggga tttcgtggtt
    22921 tggttgtgat gttgatgatg aaaagaatgt ctttggcgtt acaggagaca tctcaacaga
    22981 ggcagctaaa atccgtgtct tggttattcc aacagatgaa gaattagtca ttgcccgtga
    23041 cgttgaacgc ttgaaaaaat aagtgaaact aaaaaaatat tcaatacaag gagttgggaa
    23101 agttattttt ccagcttctt tttctgatga aattgtccaa aaccttgcta tgattggctt
    23161 ttttgaaaaa tatggtataa tagtagtaat ttaatagatg gagttgagtt ttgaagaaaa
    23221 actttcgtgt aaaaagagag aaagatttta aggcgatttt caaggagggg acaagttttg
    23281 ctaatcgcaa atttgtggtc taccaattag aaaaccagaa aaaccatttt cgagtaggtc
    23341 tatcagttag caaaaaactg gggaatgccg tcactagaaa tcaaattaag cgacggattc
    23401 ggcatattat ccagaatgca aaagggagtc tggtagaaga tgtcgacttt gttgtcattg
    23461 ctcgaaaagg agtcgaaacc ttgggatacg cagagatgga gaaaaatcta ctccatgtat
    23521 taaaattatc aaagatttac cgggaaggaa atgggagtga aaaagaaact aaagttgact
    23581 agtttgctag gactgtctct gttaatcatg acagcctgtg cgactaatgg ggtaactagc
    23641 gatattacag ccgaatcggc tgatttttgg agtaaattgg tttacttctt tgcggaaatc
    23701 attcgctttt tatcgtttga tattagtatc ggagtgggga ttattctctt tacggtcttg
    23761 attcgtacag tcctcttgcc agtctttcag gtgcaaatgg tggcttctag gaaaatgcag
    23821 gaagctcagc cacgcattaa ggcgcttcga gaacaatatc caggtcgaga tatggaaagc
    23881 agaaccaaac tagagcagga aatgcgtaaa gtatttaaag aaatgggtgt cagacagtca
    23941 gactctcttt ggccgatttt gattcagatg ccggttattt tggccctgtt ccaagcccta
    24001 tcaagagttg actttttaaa gacaggtcat ttcttatgga ttaaccttgg tagtgtggat
    24061 acaacccttg ttcttccgat tttagcagca gtattcacct ttttaagtac ttggttgtcc
    24121 aacaaagctt tgtctgagcg aaatggcgct acgactgcga tgatgtatgg gattccagtc
    24181 ttgattttta tctttgcagt ttatgcgcca ggtggagtcg ccctatactg gacagtgtct
    24241 aatgcttatc aagtcttgca aacctatttc ttgaataatc cattcaagat tatcgcagag
    24301 cgcgaggccg tagtacaggc acaaaaagat ttggaaaata gaaaaagaaa agccaagaaa
    24361 aaggctcaga aaacgaaata aataaggagg aatctggtaa tggtagtatt tacaggttca
    24421 actgttgaag aagcaatcca gaaaggattg aaagaattag atattccaag aatgaaggct
    24481 catatcaaag tcatttctag ggagaaaaaa ggctttcttg gtctatttgg taaaaaacca
    24541 gcccaagtgg atattgaagc gattagtgaa acgactgttg ttaaagcaaa tcaacaggta
    24601 gtaaaaggcg ttccgaaaaa aatcaatgat ttgaacgagc ctgtgaagac ggttagtgaa
    24661 gaaaccgttg accttggtca tgtggttgat gctattaaaa aaatagagga agaaggtcaa
    24721 ggtatttctg atgaagtcaa ggctgaaatc ttaaaacatg aaagacatgc cagcactatc
    24781 ttagaagaaa ctggtcacat tgagatttta aatgaacttc aaatcgagga agcgatgagg
    24841 gaagaagcag gcgctgatga ccttgaaact gagcaagatc aaactgaaaa tcaagacttg
    24901 aaagagatgg gcttgaaggt cgagcaaagt tatgatattg cccaggtggc tacggatgtg
    24961 actgcttatg ttcaaacgat tattgatgac atggatgttg aggctacact ttcaaatgat
    25021 tataaccgtc gtagcatcaa tttgcaaatt gatactaacg aaccaggtcg tattatcggc
    25081 taccatggta aagtcttgaa ggccttgcaa ctgttggctc aaaattatct ttacaactgc
    25141 tattcaagaa ccttctatat cactattaat gtcaatgatt atgttgaaca ccgtgcagaa
    25201 gtcttgcaga cctatgcgca aaaattggcg attcgtgttt tagaagaagg acgtagtcat
    25261 caaacagatc caatgtcgaa tagtgaacgc aagattatcc accgtattat ttcacgtatg
    25321 gatggcgtga ccagttactc tgaaggtgat gagccaaatc gctatgtcgt tgtagataca
    25381 gaataagtaa aatcagggtt gacctgattt tttgctagct agaggaggtt aaactgatgt
    25441 tgaataagat aagagactat ctagactttt ctggtttgca gtaccgtagt cctgataaag
    25501 cgggagcaga gcgagagaag atgctggcat tccgccacaa aggtcaagag gcacgaaagg
    25561 cttttacaga attggctaaa gtctttcagg caaatcatcc agaatggcaa ctccaacaga
    25621 ctagccagtg gatgaatcag gcccagcgtt tgcgaccaca tttttgggtt tatctacaac
    25681 gtgaggggaa agtgacagaa cctatgatag ccttgcgttt gtatggaaat ccttctgatt
    25741 ttggtatttc cttagaggtc agtttcatcg aacgtaagaa ggatgagcaa acactgggca
    25801 agcaggccaa agttttagac attccaaccg ttaaagggat ttattatcta acctactcta
    25861 atggtcaaag tcaacggtgg gaggcgaatg aagaaaagcg tcgtacttta cgcgagaagg
    25921 tgagaagtca agaagttcga aaagttttag tgaaggtaga tgttcctatg acagaaaatt
    25981 cgtctgaaga agaaatcgta gaaggcttat tgaagtctta ttctaaaatt cttccctatt
    26041 atctagctac gagaaaataa gataatttgt aaaacatcat aaatcataca gtccaagagt
    26101 gaacagtccg ctgtgtaatt cttggtcttt ttgtttgcgc tttcgcatta tataataaac
    26161 ttacaaaaac aattcaaaag gagaacaatt atggaagtcg tttcaagtgt tctaaattgg
    26221 ttttctagca atattttgca gaatcccgca tttttcgtag gtttattggt gttgatagga
    26281 tatgcacttt tgaaaaaacc tgcccatgac gttttttcag ggtttgttaa agcaacagta
    26341 gggtatatgt tgcttaacgt gggtgctggt ggtttggtta caacctttcg tccaatctta
    26401 gcagctctta actacaaatt ccaaattggt gcagcggtta tcgaccctta ctttggactt
    26461 gctgcagcaa acaacaaaat tgcagcagag tttccagatt ttgttggaac tgcaactaca
    26521 gctctattga ttggtttcgg aataaatatc ttgctcgtag ctcttcgaaa gattacgaag
    26581 gtaagaaccc tctttattac tggtcacatc atggtacaac aagctgcaac agtatctctt
    26641 atggttctat tcttagtacc acaattgcgc aatgcttacg gtacagcagc gattggtatc
    26701 atctgtggac tttactgggc agttagttca aatatgactg ttgaggcaac tcaacgcttg
    26761 actggtggtg gcggatttgc gattggtcac caacagcaat ttgcaatctg gtttgtagat
    26821 aaagtagcag gacgctttgg taagaaagaa gaaagtttag acaatcttaa attacctaag
    26881 ttcctcttaa tcttccacga tacagttgtt gcatctgcta ccttgatgct cgtattcttc
    26941 ggggccattc ttttaatctt gggtccagac attatgtcta ataaagaagt catcacttca
    27001 ggaactctat tcaatcctgc taaacaagat ttctttatgt acattatcca aacagccttt
    27061 accttctcag tttacttgtt cgttttgatg caaggtgtcc gaatgttcgt atctgagttg
    27121 acaaacgcct tccaaggtat ttcaaacaaa ttgttgccag gttcattccc agcggttgac
    27181 gttgcagctt cttatggatt tggttctcca aatgctgtct tgtcaggatt tacctttggt
    27241 ttgattggtc aattgattac aattgttttg ctcatcgtct ttaaaaatcc gattcttatt
    27301 attacaggat ttgtaccagt gttctttgac aatgcagcca ttgcggtcta cgctgataaa
    27361 cgcggcggat ggaaagcggc tgttatcctt tcctttatat caggtgtcct tcaagttgct
    27421 ctaggagctc tttgtgtggc ccttctcgat ttggcatctt atggtggcta ccatggaaat
    27481 atcgactttg aattcccatg gcttggattt ggatatatct tcaaatacct tggtattgtt
    27541 ggttatgtac ttgtgtgtct cttcttgctt gttattcctc aacttcaatt tgccaaagca
    27601 aaagataaag agaaatatta caacggtgaa gttcaagaag aagcttagta tctagaaaag
    27661 gagaaataaa atggttaaag tattagcagc gtgcggaaat ggaatgggtt catcaatggt
    27721 tatcaagatg aaggttgaaa atgctctccg taagcttaat caaacagatt ttacagtcaa
    27781 ttcatgcagt gtcggtgaag ctaaaggttt agcagtagga tatgacatcg taatcgcttc
    27841 tcttcatttg attcaagaat tggaagggcg aactaatggg aagttaattg ggcttgataa
    27901 cttgatggat gataaagaaa tcaccgaaaa actcagtcaa gcactacagt aaaaggttgg
    27961 agggggctgg acagaaactg agagttatcg tttctgtcct tctccctctt taaataaagg
    28021 aggcagatat gaatttaaaa caagctttaa ttgacaatga ctcgatccga ctaggtttag
    28081 aggctaacaa ttggaaagaa gcagtcaagg tagcagtaga tcccttaatt gaaagtgggg
    28141 caattttgcc agagtattac gatgctatca ttgaatcgac tgaagagtat gggccttact
    28201 atatcttgat gccaggtatg gctatgcccc acgctagacc tgaagctggt gtgcaaagtg
    28261 atgccttttc attgattacc ttacaaaatc ctgttgtatt ttcagatggg aaagaggtat
    28321 ctgttttgtt ggcactagca gcaacaagct caaaaattca cacaagtgta gccattccac
    28381 aaattattgc cctgtttgaa ttagaagatt ctattgcacg tttacaggct tgccagacta
    28441 aagaagatgt cttggctatg attgaagaat ctaaggatag cccttatctc gaaggattgg
    28501 acttggaaag ttagaaagag gaataaagaa atgacaaaaa gaatacctaa tttacaagtt
    28561 gcattagacc attcagactt gcaaggagcg attaaagcag ctgtttctgt tggtcaggaa
    28621 gtagatatta tcgaagctgg aactgtttgc ttgcttcaag ttggaagtga actggctgaa
    28681 gtcttgcgta gccttttccc agataagatt attgtggcag acacaaaatg tgctgatgct
    28741 ggtggaacag ttgctaaaaa taatgcggtt cgtggagcag actggatgac ttgtatctgt
    28801 tgtgcaacca tccctactat ggaagcagct ctaaaggcta tcaagactga acgaggagaa
    28861 cgaggcgaaa tccagatcga gctttatggc gattggactt ttgaacaagc tcagctttgg
    28921 ctagatgcag gtatctcaca agctatttat caccaatctc gtgatgctct tcttgctggt
    28981 gaaacttggg gtgaaaaaga ccttaataag gttaaaaaac tcattgacat gggcttccgt
    29041 gtatctgtaa caggtggtct agatgtagat actctcaaac tctttgaagg tgttgatgtc
    29101 tttaccttta tcgcaggtcg tggaattaca gaggctgcgg atccagcagg agcagcgcgt
    29161 gccttcaagg atgaaatcaa acgaatttgg gggtaaatca tggtacgtcc aattggaatt
    29221 tatgaaaagg caaccccaac acactgtact tggctagaac gtttaaattt tgccaaggag
    29281 ttaggctttg attttgtcga gatgtctatt gacgaacgtg acgagcgttt agcaagactt
    29341 gactggagta aggaagaacg cttggaagtt gtcaaagcaa tctatgaaac tggtgttcgt
    29401 attccttcta tctgtttttc aggccatcgt cgctacccat tgggttcaaa agatccagtt
    29461 ctagaggaaa aatctctaga actcatgaaa aaatgtatcg aattagctca agacttggga
    29521 gttcgtacga ttcaattagc tggttacgat gtttactatg aggaaaagtc accccagaca
    29581 cgccaacgtt ttatcaaaaa tttgagaaaa gcctgtgact gggctgaaga agctcaggtg
    29641 gtacttgcta ttgaaattat ggatgatcct ttcatcaata gcatcgaaaa atatttggct
    29701 atagaaaaag agattgactc tcccttcctc tttgtatatc cagatattgg taatgtgtct
    29761 gcatggcata atgatatcta tagtgagttt tatcttggtc atcatgccat cgcagctctc
    29821 catctcaagg atacttatgc agtgacagaa agttcaaagg gccagttccg agatgtacct
    29881 ttcgggcaag gttgtgtcaa atgggaagaa gctttcgata ttttaaagga aaccaattat
    29941 aatggacctt tcctaatcga aatgtggtct gaaaattgtg aaacagtaga agaaacacgc
    30001 gcagccgttc aagaggcgca agcttttctc tatccactca ttaagaaagc aggtttgatg
    30061 taagatgaat caagtaatca atgctatgcg taaacgagtc tgtgatgcca atcaatcatt
    30121 gccaaaacat ggacttgtca aatttacctg ggggaatgta tctgaagtta atcgcgaact
    30181 cggtgtcatt gttatcaaac catcaggcgt ggattatgac gaattgacac ctgaaaacat
    30241 ggtagtgact gatctagatg gtaagatcct agaaggggat ttaagaccat cttccgacct
    30301 cccaactcat gtgcaattat ataaggcttg gtcagaaatt ggtagtgtgg ttcacaccca
    30361 ttcgacagaa gctgttggtt gggctcaggc aggtcgtgat attcctttct acggaacaac
    30421 ccatgcagat tatttctacg gttcaatccc ttgcgcccgt agtttgacca aggacgaagt
    30481 agaagtggcc tatgaaaaag atactggcct ggttatcgta gaagagtttg aacatcgcgg
    30541 acttaacccg gttgaagtac caggaattgt tgtacgcaat cacggtccat tcacctgggg
    30601 taaaaatcca gagaatgctg tttatcactc tgtcgtacta gaggaagtat caaagatgaa
    30661 tcgctttaca gaacaaatca atccaagagt tggacctgct ccccagtaca tactagaaaa
    30721 acactaccaa cgtaaacatg gaccaaatgc ttattatggt caaaagtaag aacgatgaag
    30781 gaggagaaaa agataaattt agctcctctt tttacatttg atttttattg agagtaaagt
    30841 tggagttgaa gtaattttga aagatttttt agaaatagcg cttgatatat atatggtaaa
    30901 ataaaaagaa ttgctgtgat atcaatagat ttgggggatt ttttaatatg gtactggata
    30961 aggcaagttg tgatttgctt caatatttga tggatcaaga aacgtccaaa acgattatgg
    31021 cgatttcgaa agatttgaaa gagtcaagaa ggaaaattta ttatcacatt gacaaaatca
    31081 atgctgctct gggtgacgag gcgcttcaca tcattagtat tccacgaatt ggtattcact
    31141 taacggaaga gcagagagat gcttgttgta aactattatc ggaagtagat tcgtacgatt
    31201 atatcatgag tgcgcatgaa cgtatgatga taatgttact atggataggt atttctaaag
    31261 aacgtattac gattgaaaaa ttgatagagt taacagaggt atctaggaat actgttctca
    31321 atgatttgaa tagtattcgt tatcaactaa ctttggaaca atatcaggtg accttgcaag
    31381 tgagcaagtc acagggatac caccttcatg cccaccctct taataaaatt cagtatcttc
    31441 aatcgcttct atatcatatt tttatggaag aaaatgccac ttttgtatct attttagaag
    31501 ataagatgaa agagaggtta gatgatgagt gtttgctttc tgttgaaatg aaccaatttt
    31561 ttaaggaaca ggttccttta gttgaacaag atttagggaa gaaaataaac catcatgaaa
    31621 taacttttat gttgcaggtt ctaccttatt tgctgttaag ctgtcataat gttgaacagt
    31681 atcaagaaag acatcaggat atagagaaag aattttcttt gataagaaaa agaatagagt
    31741 atcaggtgtc taagaaatta ggagaacggt tgtttcaaaa gtttgaaatt tctttgtcag
    31801 gacttgaagt ttctcttgta gctgttctcc tcctctccta tcgtaaagat ttggatattc
    31861 atgcagaaag tgatgatttt cggcaattaa aacttgcttt agaagaattt atctggtatt
    31921 ttgaatcaca aatccgaatg gagattgaga acaaggatga tttgttacga aatttgatga
    31981 tccactgtaa agccttgtta tttagaaaga cttacggtat tttttctaaa aatcctctaa
    32041 caaaacaaat tcgatccaag tatggagaat tatttttagc cactagaaaa tctgcggaaa
    32101 ttttagaagg agcatggttt attcggctaa cagacgatga tattgcctat ttgacgattc
    32161 atattggagg atttttaaaa tatacaccat cgtctcaaaa aaatatgaaa aaagtttatc
    32221 tcgtttgtga tgaaggtgtt gcggtttcga gacttttgct gaaacaatgc aaactttatt
    32281 ttccaaatga gcaaattgac actgtattta caacagaaca atttaagagt gtggaagata
    32341 ttgcacaagt tgatgtagtg attactacta atgatgattt ggatagcaga tttccgattt
    32401 taagggttaa tcctatcctt gaagcagaag atattttgaa aatgctagac tatcttaaac
    32461 acaatatatt tcgtaataag agcaaaagtt tcagtgaaaa tctttctagt cttatttcgt
    32521 cttatattgt agacagcaag ttggctagta agttccaaga agaggttcaa acacttataa
    32581 atcaagaaat agtagttcaa gcttttttgg aagatatttg aaggacagtc caatgatgaa
    32641 cacaaacctg tgtttttctt ggtctttttt agtgttttga agggtggtat actaatctca
    32701 aagataacaa ttatatccaa aggaggcaac atatgccaaa cgtcaaagaa attacaagag
    32761 agtcatggat tttagccact ttcccagagt ggggaacatg gttgaacgaa gaaatcgaag
    32821 aagaagtcgt acctgaaggc aactttgcca tgtggtggct aggcaactgt ggtacttgga
    32881 ttaagacacc agctggtgct aacgttgtca tggacctttg gtcaaaccgt ggaaaatcaa
    32941 ccaaaaaagt gaaagatatg gttcgtgggc accaaatggc aaatatggca ggtgttcgta
    33001 agctgcaacc aaacttgcgt gttcagccaa tggttatcga tccatttgct atcaacgaac
    33061 tagactatta cttagtttca cacttccaca gtgatcatat cgacccatac acagctgcag
    33121 caattctcaa taatcctaag ttagagcatg ttaagtttat cggtccttac cactgtggac
    33181 gaatctggga aggatggggt gttccaaaag aacgtatcat cgttgttaaa ccaggtgaca
    33241 ctatcgaatt aaaagatatg aagattcatg cagtagaatc atttgaccgt acttgcttgg
    33301 taactctccc agtgaacggt gctgatgaga caggcggtga acttgctggc ttggctgtta
    33361 cagatgaaga aatggctcaa aaggctgtta actatatctt tgaaacacca ggtggaacca
    33421 tctatcatgg tgcagattct cacttctcaa actattttgc aaaacatggt aaagacttta
    33481 aaattgatgt tgctttgaat aactatggtg aaaatccggt aggtatccaa gacaaaatga
    33541 catctatcga ccttcttcgt atggcagaaa atctgcgtac caaagtcatt atcccagttc
    33601 actatgatat ctggtctaac ttcatggctt ctactaatga gattctagaa ctttggaaaa
    33661 tgcgaaaaga tcgcttgcaa tacgatttcc atccatttat ctgggaagtt ggcggtaagt
    33721 acacttatcc tcaagatcaa cacttagtag aataccatca tccacgtggt tttgatgatt
    33781 gttttgaaca agactctaac attcaattta aagctttgct ataatataaa aatattcttt
    33841 ggaggttatc cgtatgttgc atgatacgga taattttcat ataatagtaa aatagaatgt
    33901 gtgattcaat aatcacctca aatagaaagg aaattctatg tcaaatctat ctgttaatgc
    33961 aattcgtttt ctaggtattg acgccattaa taaagccaac tcaggtcatc caggtgtggt
    34021 tatgggagcg gctccgatgg cttacagcct ctttacaaaa caacttcata tcaatccagc
    34081 tcaaccaaac tggattaacc gcgaccgctt tattctttca gcaggtcatg gttcaatgct
    34141 cctttatgct cttcttcacc tttctggttt tgaagatgtc agcatggatg agattaagag
    34201 tttccgtcaa tggggttcaa aaacaccagg tcacccagaa tttggtcata cggcagggat
    34261 tgatgctacg acaggtcctc tagggcaagg gatttcaact gctactggtt ttgcccaagc
    34321 agaacgtttc ttggcagcca aatataaccg tgaaggttac aatatctttg accactatac
    34381 ttacgttatc tgtggagacg gagacttgat ggaaggtgtc tcaagcgagg cagcttcata
    34441 cgcaggcttg caaaaacttg ataagttggt tgttctttat gattcaaatg atatcaactt
    34501 ggatggtgag acaaaggatt cctttacaga aagtgttcgt gaccgttaca atgcctacgg
    34561 ttggcatact gccttggttg aaaatggaac agacttggaa gccatccatg ctgctatcga
    34621 aacagcaaaa gcttcaggca agccatcttt aattgaagtg aagacggtta ttggatatgg
    34681 ttctccaaac aaacaaggaa ctaatgctgt acacggcgcc cctcttggag cagatgaaac
    34741 tgcatcaact cgtcaagccc tcggttggga ctacgaacca tttgaaattc cagaacaagt
    34801 atatgctgat ttcaaagaac atgttgcaga ccgtggcgca tcagcttatc aagcttggac
    34861 taaattagtt gcagattata aagaagctca tccagaactg gctgcagaag tagaagccat
    34921 catcgacgga cgtgatccag tcgaagtgac tccagcagac ttcccagctt tagaaaatgg
    34981 tttttctcaa gcaactcgta attcaagtca agatgccttg aatgttgtgg cagctaaatt
    35041 accaaccttc ctaggtggat cagctgacct tgctcactca aacatgactt atatcaagac
    35101 ggatggactt caagacgatg ccaatcgctt gaatcgtaac attcagtttg gtgttcgtga
    35161 atttgcaatg ggaacaatct tgaacgggat ggcccttcat ggtggacttc gtgtatacgg
    35221 tggaactttc ttcgtcttct ctgactatgt gaaggcagct gtccgcttgt cagccttaca
    35281 aggacttcct gtgacttatg tctttaccca tgattcaatc gcagttgggg aagatggtcc
    35341 gactcacgaa ccagttgagc atttagcagg tcttcgtgct atgccaaatc taaatgtttt
    35401 ccgtccagca gatgcgcgtg aaactcaagc agcttggtac cttgcagtga caagtgagaa
    35461 aacaccaact gcccttgtct tgacacgtca aaacttgact gttgaagatg gaacagactt
    35521 cgacaaggtt gctaaaggtg cttatgttgt atatgaaaat gcagccgact ttgataccat
    35581 cttgattgcg acaggttcag aggttaatct tgctgtctca gctgctaaag aattggctag
    35641 tcaaggcgaa aaaatccgcg tagtcagcat gccatctaca gatgtctttg ataaacaaga
    35701 tgcagcttac aaggaagaga ttcttccaaa tgcagtccgc cgtcgtgttg cagtcgaaat
    35761 gggtgcaagt caaaactggt acaaatatgt tggtctcgat ggtgccgttc taggtattga
    35821 taccttcgga gcctctgccc cagcaccaaa agtattggca gaatatggct ttactgtaga
    35881 aaatcttgta aaaattgttc gaaacttgaa ataatcctaa aaatcagggc gtaagctctg
    35941 gtttttctta ccagaaaagt aaggtacaat cttgtaaaag tagctgaaat ttgatatagt
    36001 agtcctatgt aaaagacaaa ggagaagaca atgaatccaa atattacttt tttaatcatg
    36061 cttgtaggta tgatggcctt gatgttcttt atgcaacgtt ctcaaaagaa acaagctcaa
    36121 aagcgtatgg aaagcttaaa taaactacaa aaaggctatg aagtgattac aatcggtgga
    36181 ctttatggaa cagtcgatga agtagatacg gagaagggaa caatagttct tgacgtagat
    36241 ggagtttact tgacttttga actagctgct atcaagacag tattaccgct gaaagaaaca
    36301 gcttcactag aaggcgcaat tgaaaaataa gacgggatca cgaactcccg tttttctata
    36361 aaagaaagga aatgggatga aaaaattagt ctttgtctgt ctgggaaata tttgccgtag
    36421 ccctatggcc gagtttgtta tgaaatcaat gacagataac tacgaaatcc aaagtcgagc
    36481 aacttcctct tgggaacatg gcaatccgat tcataagggg actcagggaa tttttcaaga
    36541 gtatgagatt ccttatgaca agaacaagac atcgcttcag attagtaagg aagattttga
    36601 agcctttgat tatattatcg gaatggacgc ttcaaatatt tctgacttac gtcagatgtg
    36661 tccagtagac tgtcaagata agatttactc attttcatct gaaagtgttc cagacccttg
    36721 gtatacagga gattttgaag aaacctatcg acgtgttcaa gagggctgtc aagcttggtt
    36781 agaacgttta gaaaaggaga gttaaggtgg aaaatcttac gaatttttac gaaaagtatc
    36841 gtgtctatct gactcgtcca cgtttagagc ttttggcagt agttaccatt gttttctgtg
    36901 ctgtactcgt cttttttcta aatattccag gaaaaggtgt cttaaaactc gataatggaa
    36961 cgattgttta tgatggcagt cttgtccgtg gtaaaatgaa tggccaaggt accattacct
    37021 tccaaaatgg agaccaatat acaggtggct tcaacaatgg agccttcaac ggaaaaggta
    37081 cctttcaatc taaagaaggc tggacctacg aaggtgattt tgtaaatggt caggctgaag
    37141 gaaaagggaa actaacaaca gaacaagaag tcgtttatga aggaactttt aaacaaggcg
    37201 tttttcaaca aaaataaagc ctccttatca aaggaggtat tattagaatc acaaggtaag
    37261 cgcttacctg taaatccctt tctttccaaa tccctcttcc aagcaagttt gtgaaataaa
    37321 aaatatttga aataaatttc acaaacttca aagataaaac ttgataagaa aagaaaatga
    37381 gaaaagtttc acaagggttt aaaaattctt tgtgaaatgt ttatgaaact gtttacaaag
    37441 ttgaaataat gcgttataat aaacttgtga aaaaattaac aaaggatatc gttccttgaa
    37501 agctatggag gaaaatatgg ctgataaaaa aactgtgaca ccagaggaaa agaaactcgt
    37561 tgctgaaaaa cacgtagatg agttggttca aaaagctcta gttgcccttg aagaaatgcg
    37621 taaattggat caagaacaag ttgactacat cgttgccaaa gcatcagtag cagctttgga
    37681 tgcccacgga gaattggctt tacatgcctt tgaagaaaca ggacgtggtg tatttgaaga
    37741 caaagcaact aagaacttgt ttgcttgtga acacgtagta aacaatatgc gccacactaa
    37801 gacagttggc gttatcgaag aagacgatgt aacaggattg actcttattg ctgaaccagt
    37861 tggtgttgtt tgtggtatta ctccaacaac aaacccaaca tcaacagcaa tcttcaaatc
    37921 attgatttca ttgaagacac gtaacccaat cgtctttgcc ttccatccat cagcacaaga
    37981 atcatctgct catgcagctc gtatcgtccg cgatgcagct atcgcagctg gtgctcctga
    38041 aaactgtgtg caatggatta ctcaaccatc tatggaagca acaagtgccc ttatgaacca
    38101 cgaaggtgtt gcgacaatcc ttgcaacagg tggtaatgcc atggttaagg cggcttattc
    38161 atgtggtaaa ccagctcttg gggtaggtgc cggaaacgtt ccagcttatg ttgaaaaatc
    38221 agcaaacatt cgtcaagcag cacacgatat cgtcatgtct aaatcatttg ataacggtat
    38281 ggtctgtgca tctgaacaag cagttatcat tgataaagaa atttacgatg aatttgtagc
    38341 agagttcaaa tcttaccaca cttactttgt aaacaaaaaa gaaaaagctc ttcttgaaga
    38401 gttctgcttc ggcgtcaaag caaacagcaa aaactgtgct ggtgcaaaat tgaacgctga
    38461 catcgttggt aaaccagcaa cttggattgc agaacaagca ggatttacag ttccagaagg
    38521 aacaaacatt cttgctgcag aatgtaaaga agttggcgaa aatgagccat tgactcgtga
    38581 aaaattgtca ccagttattg cagttttgaa atctgaaagc cgtgaagatg gtattactaa
    38641 ggctcgtcaa atggttgaat ttaacggtct tggacactca gcagctatcc acacagctga
    38701 cgaagaattg actaaagaat ttggtaaagc tgttaaagct attcgtgtta tctgtaactc
    38761 accttctact tttggtggta tcggggacgt ttacaatgcc ttcttgccat cattgacact
    38821 tggatgtggt tcttacggac gcaactcagt tggggataac gttagtgcca ttaacctctt
    38881 gaatatcaaa aaagtcggaa gacggagaaa taacatgcaa tggatgaaac ttccttcaaa
    38941 aacatacttt gaacgtgatt caattcaata ccttcaaaaa tgtcgtgacg ttgaacgtgt
    39001 catgatcgtt actgaccatg ccatggtaga gcttggtttc cttgatcgta tcatcgaaca
    39061 actggacctt cgtcgcaata aggtcgttta ccaaatcttt gcggatgtag aaccggatcc
    39121 agatatcaca actgtaaacc gtggtactga gattatgcgt gccttcagac cagataccat
    39181 catcgcactc ggtggtgggt ctccaatgga tgctgccaaa gtaatgtggc ttttctacga
    39241 gcaaccagaa gtggacttcc gtgaccttgt ccaaaaattc atggatatcc gtaaacgtgc
    39301 cttcaagttc ccattgcttg gtaagaagac taaattcatc gcgattccaa ctacatctgg
    39361 tacaggatct gaagtaacac catttgccgt tatctctgat aaagcaaaca accgtaaata
    39421 cccaatcgct gactactcat tgacaccaac tgtggcaatt gtagaccctg ctttggtatt
    39481 gacagttcca ggaattgttg ctgctgatac tggtatggac gtattgactc acgcgacaga
    39541 agcatacgta tcacaaatgg ctagtgacta cactgatggt ttagcacttc aagctattaa
    39601 attggtcttt gaaaatctcg aaagctcagt taagaatgca gacttccact cacgtgagaa
    39661 aatgcataac gcttcaacaa tcgctggtat ggcctttgcc aatgccttcc taggtatttc
    39721 tcactcaatg gcccataaga ttggtgcgca attccacaca gttcacggac gtacaaatgc
    39781 catcttgctt ccgtacgtta tccgttacaa cggtacacgt ccagctaaga cagcaacatg
    39841 gcctaagtac aactactacc gtgcagatga aaaataccaa gatatcgcac gcatgcttgg
    39901 acttccagct tctactccag aagaaggggt tgaatcttac gcaaaagctg tctacgaact
    39961 cggtgaacgt attgggatcc aaatgaattt tagagaccaa ggaattgacg aaaaagaatg
    40021 gaaagaacat tctcgtaaat tagccttcct ggcttatgaa gaccaatgtt caccagctaa
    40081 cccacgtctt ccaatggtag accatatgca agaaatcatc gaagatgcat actatggcta
    40141 caaagaaaga ccaggacgcc gtaaataatt gtttatcagt ctagaagcaa gacaaaaact
    40201 caatttgagg gaaagatcca gtaatttttc tatgataaaa ggcatcctat caaggttttt
    40261 gaatacctga taggatgcct ttttatgatg ttgaggcctt tttgcccttt ttgaaaaact
    40321 agaatagaaa caaaatatat agtaggttga aactagaata gtacacatct gctgctaaaa
    40381 catttataga aattaatttt actttcctga tagagttgtt cacatcttat ttcaattcac
    40441 tatagtttaa tttaagagta gtatttacta aggcccaatt aaaatcaaag agcaaactag
    40501 aaaacgagaa aacgatgaca aggtgtgttg ctttttgatt tctaaagagt ataatgatag
    40561 atctctataa aataagtgcg aaggaaatga gcttttataa tcctttcgtt ttaaaatact
    40621 atctcagata ttcttatatc gacaagaagt ttttgagtca ttccctcatc atacatatta
    40681 aataaatagt ggctcattca attttttact agaataataa gctagtatag taaactgaaa
    40741 taagatataa acaaataaat tggagcttaa catctatttc caggaatttt ttagaaactg
    40801 cagtggacta ttctaaattc aacacattat aaaaactaga gtaaaagaaa aggattggat
    40861 cttgtgtaat gcaggatcca atcctttcaa tcattttgtc caacttttgg aggttcctac
    40921 aatgtagccg tcattaataa agacagatgg gaacgacagt gttcctattt attttgatag
    40981 agatcgatga attctttaga tagcaactga ataatctctg ttgaagccat ttggtcttct
    41041 gcatgcataa atagcaagga gaatcctatt ttttctccag tagcttcttt ttgtatgaga
    41101 ttagagtgaa tcttgtgcgc ttctactaag gagtcttccg cttcttcaac tttaattttc
    41161 gcttctttta aatttcctgc cttagctagt tggatggctt caataaagga tgatttggct
    41221 gctccactat tggcaatgag ctgaaaacag atatattcca tttcttctgt catcttattt
    41281 ctcctatcca tgcaagtgct tgttccagaa cttttgctcc attcatcatt ccgtaatccc
    41341 gcatatcaat ggtatctaca gggatatttc ctgcaatttc tttcacagca agtaactcat
    41401 aacgaatttg tggcccaatt agaatgacat ctgcttcatg gatattcttt ttagcttctg
    41461 tcattgattt tgcttggata gagatttcaa tcccacgttc agtcgcactt tgttgcattt
    41521 ttttaacaag catacttgtc gacattcccg cattacatac taataaaatt tgtttcataa
    41581 tcttaacctt ccatttcttg ttcaacaact ttgtcattaa ctttgataaa tggaatgtat
    41641 agaagaactc caagtgcaaa gatgatgaat tgaactagaa ctgctctcac gtcccctgct
    41701 gttgctaacc atgcatttaa gaatactggt gtagtccaag gaacttgtat aaatgcagga
    41761 ctcatgaatc ctgtaactgt tgctaagtag ctgattaaaa taccaaggac tggaactgtg
    41821 ataaatggaa tagctaatga aatgttataa acgattgggt aaccgaataa tactggttca
    41881 ttgatattga agataccagg tccaaaagat aatttagcca cgtttttaga gacagcattg
    41941 cgactcacta agaatgttgc tattaataaa cataatgtag atccactacc acccattaaa
    42001 gcgaatgttt gtatttgtga taggttgatg atgtgtggaa tggcttgtcc attatttgct
    42061 gcagtgatgt tttcagtaat gttaattaat agtaatggtt ctaggatggc actgtaaata
    42121 actgcttggt gaataccaaa tagccataac atatttccta aagagtaaat aataatgacc
    42181 ccgattaagc ttgtaccaat atgacgaatt ggttcttgaa taaagattgt aatgattgag
    42241 attaagttca ttccagttat attgaataat aatgctgaaa caaccccaaa taaggagatg
    42301 acggtcatga ctggaagtaa tacgctaaat gatctactaa cagctggtgg aatattttca
    42361 ccaaggttca tttgtaaagc tttaacattt gataattcaa tgaataattc tgttgcaata
    42421 atcgctacga taaccccggc gaacattgcg cctgtacctg tgttgttgaa tgaaagaaca
    42481 cctgaaatgt ttaccgcatc ttttgctccg tcaggaacta cagaaactgt atttggcatc
    42541 atcacaatta aagaaactaa tgatagcatt gatgctgcta acgggttttc gaaatctctg
    42601 tttttagcta agaaataacc aaccattaca gcaataatca tacctgaaat acttaaagta
    42661 ccgtttgcaa ttgttattcc ccaatattgg aatcttgtta atgtatcccc ttggaaaatc
    42721 cacttaaata ccgtgttgtt caaaagaacg attaaacctg ccaaaatata taatggcatt
    42781 actgttacga atgcatctct tagggttttt aaatgaattt ggttccctag tttaccagca
    42841 aaggtggcaa attggtctat aaattttgag tttttcatat gaatatctcc tctctttact
    42901 cctcaatttc tacttcaaaa ccgttattgt cacttacttt gcgataccat cttcctgagc
    42961 tcttgatggt tctcttttgc gtttctaaat caacggagat aaatccgtaa cgattcttat
    43021 atgcattatt ccaagaccag caatcaaatg cggtccaagc gtggtatcca aaacagtgac
    43081 ttccctcttc aatggcttta tgtagccatc ttaaatgttc ttcataaaat tcaatacgat
    43141 acacgtcatc gataactcca ttttcatcga taaaccgtgc ttcgttttca acacccattc
    43201 cgttttcact gataaaccat gggatattac catattcttc cttcacaatc atagcaatat
    43261 cataaatggc tttcggaaaa atttcccaac cacgatatgg attcatgcga cgctccagcc
    43321 attcatactc tttgaagtat tggtctggca tccagggcgt ctgatattcc tcaggatttg
    43381 cttgtgcttg aacacgtttt ggatggtagt agtttactcc aagaaaatca accgtatttg
    43441 atttcatcag ttgaagctct ttttcggtat gactccataa cacgccatct ctctctagct
    43501 gttttaccaa tctttctggg aaagttcctt taacagctgg attcaagaag actttgttaa
    43561 agaagtcatc tgtaaatcga cttgcttcta agtcttctgg agaattactt cttggataag
    43621 caggtgtcaa gtttaaaata atcccaatct ttccatcaag tcctaatgag cgatatagtt
    43681 gaatcacttt tgcactagca aggtttagat tatagatgac ttgtacggcc tcttttccct
    43741 ttccttttag atttggataa tggaaagcat ataagtatcc tgcttctgga atgaccattg
    43801 gctcattgaa agttgtccag taatgaacct tatctccaaa gcatgtgaaa gcagtcttgg
    43861 caaacttcac gaataactcc actacatgtt tgctttccca accaccgtat ttttgaagaa
    43921 gttccactgg taaatcaaaa tgatgtaaat tcatcacaag atccatctgg ttctttttag
    43981 cttcttcaat gatggcattg tagaaagcaa taccttttgg atcaggctca cccgagaagt
    44041 ttttgagtct tttcttgtaa cacatatagt atactgaggt tagaatagta gactgtgact
    44101 tctaaaaaat tgctagaaat gaatttcaat ctcccaattt atttgttcat atcttctttt
    44161 aatatattaa acaaattctg aatcataatc atttaaaaaa attttatttt tttatttttc
    44221 attacgaata atatagatga aggggaaaga gtatgaaaac agaactgttt cttttgctat
    44281 tagttcaaaa ggagaaaaaa tgaaagtaga aaatatttcg tatagggtgg atcatcgtat
    44341 attgtttgat aatatttctt ttgatacttc gagttcaggc gtgacattaa ttactggtaa
    44401 aaatggtaca ggaaagtcaa ctttactata gtagattgaa actagaatag tacacatctg
    44461 cttctaaaat attgttagaa atcgatttga ctatcctgat ctatttctcc tgttcttatt
    44521 tcatttcact acatctcaaa ttgagtatga cgaagtgcgc tcccatgtcc tgggaacgca
    44581 ctttcttcat atttttcata ttcttgaatc catcgataaa gactattggg atgagttttt
    44641 aaagttgaac taatcatttt tacaggatga ggtttacagc agagagtttg aaggctttat
    44701 caaaggtttt tcttggcata atgacttttc ctcgtttccg cttaattttg tgtctacttt
    44761 atgataccaa gtccaccctt aagttagata ataaatctaa cttaaggaag ctagaaggat
    44821 gagaatccag gtggtcaaga gtcccaaact taagctgatg gggacaccca gaataatttg
    44881 ctttttgaag gcaaggccac gttcctctat attgggaagt gagagttgaa tgagagaacc
    44941 agctgatgaa aagggtgaga tattagtaga tagagcgcca ataacggtgg ctgttgtgag
    45001 taagtgaata tcaatctgag gattttgagc actgatgata gcaatgatgg gaaagagggc
    45061 tggagctaca acggataggg tggaactaaa gagtgacatc actccggcta tcacacaaaa
    45121 gaacagaggt aaccagaaat gaggaatgga tgttgtcatg aggtgcccta tcagtgtgac
    45181 taaacctgac ttgaccgcta gagacattag taagctcatg ccgcagagca tgataattgt
    45241 agcccaggga actttagcca aaatggcttc ttgcttccct aatttgagcc ttaaggcgag
    45301 gcagaccata agtattgaga caaagccaat atcaaatgtt ttttgataag tagctatcca
    45361 ggcgatgttt gggaaaatga gatgcaacaa gggaaaaagc caaaccaaaa ccatgctgct
    45421 gatcatgagc aaggtggttt gtctttgaac cttgctgagg agtggtggtt ggtcaatagt
    45481 caaggatgag tttgttcttc ccttactata gtgactgtaa caggataata aaagcaagac
    45541 gatgagtggg tagataatgc tgacgataaa gatatgattg ccaagtgaaa aagcttgatc
    45601 ttcccatccc atttgcttaa acaggccttg aaagacaatg cctgagctac tggttatcaa
    45661 attagcccct cctgaagctc cccaattgac ggcttgagct ccaatcaaag ggtgtttgtc
    45721 cgctttttga cagagggtaa tcgctagagg acagcaaacg gccatagtag tgaaaaatcc
    45781 agcacctaaa gcagacaaaa gggttgccat caggtataaa atcatgtaga gggcgttagg
    45841 gtaggtgcgt gtgcggtaga gaatgtgttg agccaaaaca tcaagagtac cgttagttat
    45901 tgcaaagtta taaaagagag agacgctaaa aatggtaaaa aagagtgagg ttggccaaaa
    45961 atgaagaagt tctttggggc ttaatcccat gagagtggtt gcgatgaggt aagaaaaagc
    46021 aatagccagc aggccaatat tgattttggt gcggtaacca attccaatgg ctagagcaat
    46081 ggcgctaatc attattaaat gaatcattga attgtccttt cagttagaat atggaaatag
    46141 gttggcaatt ttggcgctca aaagaccacc tgtttcctat tggtacttga ctattataca
    46201 caaaaaaaat acaattgtct tgacaattgt attgacactt gttagaatta aaaatgaggt
    46261 attcaggctt gtgatttcct atggaagtta atagtgattg cctctaatgc ttacaagtga
    46321 tattaaaaat agaggaccta gtgatgtcaa tcatttcaac tgatttaacc ccttttcaaa
    46381 tagatgatac attgaaagca gccttgcgag aagatgttca ttccgaagat tacagtacca
    46441 atgccatttt tgatcatcat ggccaagcca aggtgtcgct ttttgccaag gaagctggtg
    46501 ttttagcggg gctaaccgtt tttcaaaggg tttttaccct atttgatgcc gaggtgacct
    46561 tccagaatcc tcatcaattt aaggatgggg atcgtttgac tagtggcgat ttggttttag
    46621 aaatcatagg ctcggtgaga agtctcttaa catgtgaacg cgttgccttg aattttttac
    46681 aacatttatc agggattgct tcgatgacag ctgcttatgt agaagcctta ggcgatgatt
    46741 gcattaaggt atttgatact cgaaaaacta ctcctaattt acgtcttttt gagaaatatg
    46801 ccgtgagagt tggcggtggc tataatcatc gctttaattt atcagatgct atcctgctaa
    46861 aagacaatca cattgcggca gtaggtagtg ttcaaaaggc aattgctcaa gcgcgtgcct
    46921 atgccccttt tgtgaaaatg gtcgaggtgg aagtggaaag ccttgctgct gccgaagaag
    46981 ctgcggcggc gggtgctgat attatcatgt tggataatat gtcattggaa cagattgaac
    47041 aggccattac cctaattgca ggacgttctc ggattgaatg ttctggaaat attgatatga
    47101 ccactattag ccgttttcgt ggtttagcga ttgattacgt ctccagtggt agtttaaccc
    47161 atagtgctaa gagtcttgat ttttccatga aggggttaac ctaccttgat gtctaagttg
    47221 taaaataaac taacttttta aaggatgtct ttcctctaga acgagtttta tgtcagatag
    47281 tttaaacgcc tcttcaaata tagtaaaatg aaccaaaaat agtacacaat g
//
LOCUS       DAVDWT010000012        46919 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVDWT010000012 DAVDWT010000000
VERSION     DAVDWT010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.012"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..495
                     /locus_tag="VUW50_000827"
     CDS             <1..495
                     /locus_tag="VUW50_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7982754.1"
                     /translation="FHRKSHEYKAIKRYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEI
                     LDKLLSYSQDLKHHYQLYQLLLFHFQNKKPEKFFGLIEDNLKQVHPLFQTVFKTFLKD
                     KEKIVNALQLPYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFV
                     LSRA"
     gene            complement(670..1974)
                     /gene="eno"
                     /locus_tag="VUW50_000828"
     CDS             complement(670..1974)
                     /gene="eno"
                     /locus_tag="VUW50_000828"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET7982755.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFLGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2138..2584
                     /locus_tag="VUW50_000829"
     CDS             2138..2584
                     /locus_tag="VUW50_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HET7982756.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(2581..3697)
                     /locus_tag="VUW50_000830"
                     /pseudo
     CDS             complement(2581..3697)
                     /locus_tag="VUW50_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(3706..3939)
                     /locus_tag="VUW50_000831"
     CDS             complement(3706..3939)
                     /locus_tag="VUW50_000831"
                     /inference="COORDINATES: protein motif:HMM:NF019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET7982757.1"
                     /translation="MISPQVKKETLEKWRKKLKLSKERTVAIGDGVNNLLMLKSAELG
                     IAFCSKEMLKKEIPHHVDKRDFLEVLPLIDCLE"
     gene            complement(3979..4350)
                     /locus_tag="VUW50_000832"
     CDS             complement(3979..4350)
                     /locus_tag="VUW50_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HET7982758.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVPGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4409..5842)
                     /gene="glgA"
                     /locus_tag="VUW50_000833"
     CDS             complement(4409..5842)
                     /gene="glgA"
                     /locus_tag="VUW50_000833"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET7982759.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(5839..6978)
                     /gene="glgD"
                     /locus_tag="VUW50_000834"
     CDS             complement(5839..6978)
                     /gene="glgD"
                     /locus_tag="VUW50_000834"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HET7982760.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(6968..8110)
                     /locus_tag="VUW50_000835"
     CDS             complement(6968..8110)
                     /locus_tag="VUW50_000835"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET7982761.1"
                     /translation="MKNEMLALILAGGQGTRLAKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGIDSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLELGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8100..10028)
                     /gene="glgB"
                     /locus_tag="VUW50_000836"
     CDS             complement(8100..10028)
                     /gene="glgB"
                     /locus_tag="VUW50_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET7982762.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEYGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATNITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
     gene            complement(10477..11901)
                     /locus_tag="VUW50_000837"
     CDS             complement(10477..11901)
                     /locus_tag="VUW50_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HET7982763.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     SLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(12445..14724)
                     /gene="pulA"
                     /locus_tag="VUW50_000838"
     CDS             complement(12445..14724)
                     /gene="pulA"
                     /locus_tag="VUW50_000838"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HET7982764.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEENLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(14836..16794)
                     /gene="ligA"
                     /locus_tag="VUW50_000839"
     CDS             complement(14836..16794)
                     /gene="ligA"
                     /locus_tag="VUW50_000839"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET7982765.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(16887..18065)
                     /locus_tag="VUW50_000840"
     CDS             complement(16887..18065)
                     /locus_tag="VUW50_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7982766.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAEVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(18145..19008)
                     /locus_tag="VUW50_000841"
     CDS             complement(18145..19008)
                     /locus_tag="VUW50_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET7982767.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(19011..20912)
                     /locus_tag="VUW50_000842"
     CDS             complement(19011..20912)
                     /locus_tag="VUW50_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET7982768.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKFSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
     gene            complement(21342..21617)
                     /locus_tag="VUW50_000843"
     CDS             complement(21342..21617)
                     /locus_tag="VUW50_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET7982769.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(21724..22563)
                     /locus_tag="VUW50_000844"
     CDS             complement(21724..22563)
                     /locus_tag="VUW50_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET7982770.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            22705..23553
                     /locus_tag="VUW50_000845"
     CDS             22705..23553
                     /locus_tag="VUW50_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET7982771.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(23646..24563)
                     /locus_tag="VUW50_000846"
     CDS             complement(23646..24563)
                     /locus_tag="VUW50_000846"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET7982772.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            25001..25206
                     /locus_tag="VUW50_000847"
                     /pseudo
     CDS             25001..25206
                     /locus_tag="VUW50_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(25517..25810)
                     /gene="rpmA"
                     /locus_tag="VUW50_000848"
     CDS             complement(25517..25810)
                     /gene="rpmA"
                     /locus_tag="VUW50_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET7982773.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(25827..26171)
                     /locus_tag="VUW50_000849"
     CDS             complement(25827..26171)
                     /locus_tag="VUW50_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HET7982774.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(26187..26501)
                     /gene="rplU"
                     /locus_tag="VUW50_000850"
     CDS             complement(26187..26501)
                     /gene="rplU"
                     /locus_tag="VUW50_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET7982775.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(26537..26624)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(26689..27387)
                     /locus_tag="VUW50_000851"
     CDS             complement(26689..27387)
                     /locus_tag="VUW50_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HET7982776.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTNHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKA"
     gene            27789..28088
                     /locus_tag="VUW50_000852"
     CDS             27789..28088
                     /locus_tag="VUW50_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET7982777.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
     gene            complement(28429..29403)
                     /gene="pta"
                     /locus_tag="VUW50_000853"
     CDS             complement(28429..29403)
                     /gene="pta"
                     /locus_tag="VUW50_000853"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HET7982778.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(29447..30343)
                     /locus_tag="VUW50_000854"
     CDS             complement(29447..30343)
                     /locus_tag="VUW50_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET7982779.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(30340..31158)
                     /locus_tag="VUW50_000855"
     CDS             complement(30340..31158)
                     /locus_tag="VUW50_000855"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HET7982780.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(31142..31813)
                     /locus_tag="VUW50_000856"
     CDS             complement(31142..31813)
                     /locus_tag="VUW50_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HET7982781.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            31941..32510
                     /locus_tag="VUW50_000857"
     CDS             31941..32510
                     /locus_tag="VUW50_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HET7982782.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            32623..33582
                     /locus_tag="VUW50_000858"
     CDS             32623..33582
                     /locus_tag="VUW50_000858"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET7982783.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            33592..34707
                     /locus_tag="VUW50_000859"
     CDS             33592..34707
                     /locus_tag="VUW50_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET7982784.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            34712..35059
                     /locus_tag="VUW50_000860"
     CDS             34712..35059
                     /locus_tag="VUW50_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HET7982785.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            35067..35282
                     /locus_tag="VUW50_000861"
     CDS             35067..35282
                     /locus_tag="VUW50_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET7982786.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            35365..36006
                     /locus_tag="VUW50_000862"
     CDS             35365..36006
                     /locus_tag="VUW50_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET7982787.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            36024..36713
                     /locus_tag="VUW50_000863"
     CDS             36024..36713
                     /locus_tag="VUW50_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET7982788.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(36710..37390)
                     /gene="radC"
                     /locus_tag="VUW50_000864"
     CDS             complement(36710..37390)
                     /gene="radC"
                     /locus_tag="VUW50_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET7982789.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(37433..39724)
                     /gene="pcrA"
                     /locus_tag="VUW50_000865"
     CDS             complement(37433..39724)
                     /gene="pcrA"
                     /locus_tag="VUW50_000865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HET7982790.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTTYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            complement(39915..40719)
                     /locus_tag="VUW50_000866"
     CDS             complement(join(39915..40349,40351..40719))
                     /locus_tag="VUW50_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HET7982791.1"
                     /translation="MNDEASKQLTDARFKRLVDVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAADLGIHESNLIRRSQWVEVTLVQSGFTISR
                     TPLSSEDTVMIDATEVKTNRPKKELANYSGKKKFHAMKAQAIVTSQGRIVSLDITVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSCKLKPLTVEDKAYNHA
                     LSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNFIAGIINHELGF"
     gene            complement(40765..40875)
                     /locus_tag="VUW50_000867"
                     /pseudo
     CDS             complement(40765..40875)
                     /locus_tag="VUW50_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase"
     gene            complement(41100..41960)
                     /locus_tag="VUW50_000868"
     CDS             complement(41100..41960)
                     /locus_tag="VUW50_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HET7982792.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            complement(41976..43253)
                     /locus_tag="VUW50_000869"
     CDS             complement(41976..43253)
                     /locus_tag="VUW50_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7982793.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            complement(43246..43797)
                     /locus_tag="VUW50_000870"
     CDS             complement(43246..43797)
                     /locus_tag="VUW50_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7982794.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            complement(43813..45072)
                     /locus_tag="VUW50_000871"
     CDS             complement(43813..45072)
                     /locus_tag="VUW50_000871"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET7982795.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            46274..46919
                     /locus_tag="VUW50_000872"
                     /pseudo
     CDS             46274..46919
                     /locus_tag="VUW50_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        14309 a        10150 c         8322 g        14138 t
ORIGIN      
        1 tttcatcgaa aatcccatga atacaaggct atcaagcgct actggaagct catacaacag
       61 gatagccgta aactgagtga taagcgattt tatcgtccta cttttcgtat gcacttaacc
      121 aataaagaga ttttagacaa gcttttgagc tattcacaag acttgaaaca ccactatcag
      181 ctctatcaac tcttgctgtt tcactttcag aataagaaac cggagaaatt tttcggactc
      241 attgaggaca atctaaagca ggttcatcct ctttttcaga ctgtctttaa aacctttcta
      301 aaggacaaag agaaaatcgt caacgccctt caactaccct attcaaacgc caaattggaa
      361 gcgaccaata atctcatcaa acttatcaag cgcaatgcct ttggttttcg gaactttgaa
      421 aacttcaaaa aacggatttt tatcgctctg aatatcaaaa aagaaaggac aaaatttgtc
      481 ctttctcgag cttagctttt tttcaaccca ctacagttga caaagagcct aaaaagtttt
      541 gtcccttttt tgccctctaa atacaaaaat agcccttcgg ataaaatccg aggggctaga
      601 aacgttgtta aatcaacggc cgaacttttg aatttcatgg ttcgggataa aatagttcac
      661 tgaactattt tattttttaa ggttgtagaa tgatttcaat ccacgatatt cagctacttc
      721 accaagttgg tcttcgatac gaagcaattg gttgtattta gcgatacggt ctgtacgtga
      781 aagtgaacca gtcttgattt gtcctgcgtt agttgcaact gcgatatcag cgattgttga
      841 atcttcagtt tcacctgaac ggtgtgatac aacagcagtg taaccagctt ctttagccat
      901 ttcgatagct tcaaaagttt cagtaagagt accgatttgg ttaactttga taaggattga
      961 gttagcagca ccttcttgga taccacgtgc aaggtagtca gtgtttgtta cgaagaagtc
     1021 gtcaccaaca agttgtactt tcttaccaag acgttcagta agagctttcc aaccatccca
     1081 gtcgttttca tccataccat cttcaatagt gatgattggg tatttgttaa ccaattcttc
     1141 aaggtagtcg atttgttctg cagatgtacg aacagcagca ccttcgcctt caaatttagt
     1201 gtagtcgtaa actttacgtt ctttatcgta gaattctgat gaagcacagt caaatccgag
     1261 aaatacgtct ttacctggta catatccagc agcttcaatc gcagcaagga tagtttcaac
     1321 accatcttca gttccttcga aacgaggagc gaatccacct tcgtcaccta cggcagtttc
     1381 caaaccacgt gatttaagga ttttcttaag agcgtggaag atttcagcac cgtaacgaag
     1441 ggcttcttta aatgttggcg caccaactgg caagatcatg aactcttgga aagcgattgg
     1501 agcgtcagag tgagaaccac cgttgatgat gttcatcatt ggagttggaa gaactttagt
     1561 gttgaatcca ccaaggtagc tgtaaagtgg gatttcaagg tagtcagcag cagcacgagc
     1621 tacagcgata gacacaccga ggattgcatt cgcacccaat ttacctttgt taggagtacc
     1681 gtcaagtgcg atcatagcac ggtcaatagc ttgttgatca cgtacatcgt agccaatgat
     1741 agcttcagca atgatgttgt ttacgttgtc aacagctttt tgtgtaccaa gaccaccgta
     1801 acgagatttg tcaccgtcgc gaagttcaac tgcttcgtgt tcaccagtag aagctcctga
     1861 tggaaccata ccacgtccga aagcacctga ttcagtgtaa acttctactt caagtgttgg
     1921 gttaccgcgt gagtctagga cttcgcgagc gtaaacatca gtaataattg acatttttta
     1981 ctctccttat gagttaaatt ttttacacct ctataatacc ttaaaacccc tcctttttca
     2041 agaaaaaacg ttatctttgt gcaacttttc cttaacttta taaagtaatc gctttctttt
     2101 gtctgtttta ttctaacttt tatgatatac tgttttcatg acagatttat caaaacaatt
     2161 acttgaaaaa gctcatggtg ggttaaaaat aaatccggat gagcaaagac gctatcttgg
     2221 tacttttgag gaaagagttc ttggatatgt agatattgac acagcaaata gccctcagtt
     2281 agaaaaaggc tttttattta ttttagaaaa ccttcaggaa aaagcagagc cactatttgt
     2341 gaagatttca ccaactatcg aatttgataa gcaagttttc tacttaaaag aagcaaaaga
     2401 aactgatagt caagccacca tagtatctga agagcatatt acttctcctt ttggcctggt
     2461 tattcatagc aatgcaccag ttcaagtaga agaaaaagac cttcgacttg cttttccaaa
     2521 actttgggaa gttaaaaagg aagaaccagc caaaacatcc ttatggaaga aatggtttag
     2581 ctaaatcttg tacatattta ataagtgccc aatattggca gccgtgcgct ccagatagag
     2641 actggcattt tttaaactat cttctaaagg ttcacttttc tccaaaatag aaaagacagc
     2701 ttggatattt tcaaatggta ggggaggtaa atcttcagca agactaccgc aaatagcaat
     2761 aacaggaact ccaacagggg ttctttttgc aacacctata ggcgctttcc cagcaaagct
     2821 ttgactgtca agtcttcctt ctccaacaac aagcaagtca gcatctgaaa ctttcttatc
     2881 aaagttgatt aagtccaagc aaggtatcaa ttccagacac gatacttgcc tgagcaaagg
     2941 cacacaaacc accagcaagg cctccacctg ctcctgctcc tttaatttct aatgttgcag
     3001 gtgagaattt ttcataaaaa tcttggatcg cctgatctac gactgcaaac atagtcggat
     3061 gtagaccttt ttgattgcca aaagtgtaag tcgcaccttg atgaccacat aagggactca
     3121 cgacatctgc tagaatatga atttgaacac cttcaggaat tttatagcaa ttttctgttg
     3181 aaacagaagc taagtttaat aaggattgac tggaagcagg caagacattt ccatccctat
     3241 cataaaattg ataacctaaa ccagcagcaa tccccagtcc tccatcatta ctggccgtgc
     3301 taccaacacc gatataaata tctttaatcc ctttagaaat gagatggaga atcaactctc
     3361 caataccaca agtttggatt tgaagtggat ttcgtttctc tagcggaatt tttccaagac
     3421 caaccaagtc agctacttca aatagtgcca gttccccttt ttgaaaatag cgcatggctt
     3481 ctttttgtcc aaaagggtct gtcacttgga tccatttttc ttttaggtca agagaatgtc
     3541 ggatagcatc tacagtacct tctcccccat caccaacagg gcagaggaga tattctacat
     3601 ctgctatcga ttgttggaag cctcttttta ttgcttcagc tacctgttga gctgtcaagc
     3661 tttccttaaa cgaatccggt gcaattacaa tcttcatatt ttccctcatt ctaaacagtc
     3721 aatcaaagga agaacttcta aaaaatccct cttgtcaaca tgatgtggta tttctttttt
     3781 gagcatttct ttggaacaaa aggcgattcc taactccgcc gacttcaaca ttaatagatt
     3841 attgacccca tcaccgattg ccaccgttct ttctttagaa agttttagtt tctttctcca
     3901 tttttccaga gtttcttttt tgacctgggg acttataatt tgtccaacta attttcctgt
     3961 taaaaacctt ctttgacttc aagctggttg gcagtgaaat aggcaatacc aagggatttt
     4021 gctaatctct ccaactattg gtgtaaatcc accaggcacc agaccaacta ggatgccatt
     4081 cttttggaga atagagatga actctgggac atttagcgat agatgaattg agttgaagac
     4141 gttatcaaag accaaaatag gaagaccttc caacaaggac actctttttc ttaaactgct
     4201 ttcaaagacc aactctcctc gcattgctcg acttgtaatc tgcgaaattt ccgcctcatg
     4261 acctgcctct ctccctaaaa gatcaatcac ttcttctagg attaaggttc catctacatc
     4321 caaaacacac aagcctttta cttgagacat cagttctcct ctctaaacag cctaaaaatc
     4381 gtatgaagtc atcatacgat tttatctatt aattaactaa actatggtac aagtcaaggt
     4441 atgacttgca ggctgtatcc catgagaagt cactctccat agcttgtttt tgtaggtttc
     4501 tccaaatgtc tggatggttt ctatacaagt ccaatgctgt ttggaaagtc caatttaacc
     4561 aataaggaga tagattgtca aagctaaagc cagtaccgct tccttcgatt ggattgaaag
     4621 cgcgaactgt atctcgcaag cctccaactt catggaccaa tggcaaggtt ccataacgca
     4681 tagccatcat ttgagacaag ccacacggtt caaaacgact tggcatgagg aagaggtcac
     4741 aagcagcgta gatttcctga gcaagtttga catcaaaagt gatatttgtt gatagcttgt
     4801 ctgggtaaat ctgagcaaac catgagaaag ctccttcaaa ggctggatcg ccagttccca
     4861 aaagaacaat ctgaacatct tcttgcaaga tatggtgaag actttcgacc accacatcaa
     4921 aacctttttg acgtgtcaaa cgagaaacaa ttcccaccag tggaacgtct gctctaacag
     4981 gcaagccaac tctttcttgc aattttgcct tatttttggc tttcccagac aaatcttcct
     5041 gattgaaatg atagtctaaa agagcatccg tctgaggatt ataaagatca gcatcaatcc
     5101 cattcacgat accagatact ttaccagact ccattttaag aatctgatcc aaattacatc
     5161 caaactgact agtcataatt tcatgagcat agctaggtga aacggttgaa acacggttcg
     5221 catagagaat acctgccttc atccagttca gacagttgtt ccatcgaagg gtgccatcag
     5281 cgtaacgttc aaagccaact ccaaacaaat cacccaacat tccttctgaa aattgtcctt
     5341 ggaattctaa attatgaatg gttaaaactg tttcaatgtc ctcataggct tgaatccaac
     5401 ggtatttttc cttcaacaag aaaggaatca tagctgtatg gtagtcatga acatggagaa
     5461 gatcaggaat aaagtcaatc ctttccatag cctcaatggc agccagttgg aaaaaggcaa
     5521 agcgttctcc gtcatcaaaa tcaccgtaaa catgaccacg gaagaaataa tattgattgt
     5581 caataaagta gaaggttaca ccatttaata ctgttttctt aattccacaa tactgtctgc
     5641 gccaaccaac gctcacctca aaatgaagca catcttcaat ctgatttcca aatttagcct
     5701 ctaccatatc atagtagggt aaaatcactg caacttcgtg cccagctttt accagtgatt
     5761 ttggaagagc gccaatgacg tctcccaaac cacctgtttt tgaaaagggt gcaccctctg
     5821 ctgctacaaa taaaattttc atgaatgaat atcctctgtt actttagcac ctttcttaac
     5881 cacaactgga tgttctgcag ttcctcgaat cacaacacca ggctcaactt caaccccttt
     5941 gtccaagata gcatattcga cctgagcccc ttctccaata acaacacgag ggaagagcag
     6001 gctatcttta accaagctat ccttatggac atgaatatta cgtgatagaa cagaattagc
     6061 tacttgacct tcaataatac taccagaggc aaactgagaa gtgcttacct tagatgtatt
     6121 agcatagtaa gttggctctt cgtttttgac ctttgtataa atcttttggt ttggtgagaa
     6181 aagagaatag aatttttgtg attcaagcat atcgatattc gcttgataat aagatttaac
     6241 agagtgaata ttggctagat agcccgtgta ctcgtaggcg aaagctccct cttttacagc
     6301 caaatcccgt aaaacatagc gcaatttctc tggatgttct tttttagctt cttcttccaa
     6361 gtgttcaatc aaccaaggtg tatcaacgac aaagatatct gtagacatat tgaacgtttc
     6421 agctgttgac ttgctatcaa agagtttatg agaaagaaca tggtctgttt catctacatc
     6481 caagattgca tttacttctg aaatatcttt cttagctagt tttttataaa ctacagtgat
     6541 aggctctttt gttgtactat gtaggtggaa aacttggttc aaatcaatgt taataagaac
     6601 atcgcagttg agggcaaccg tttggtttga gccagaacgt ttcaaataag taagaagctg
     6661 ttggtagtat tcttttccaa ctgtactact ttctacacgg gtattgtaaa ttcctagata
     6721 gtaatggcta agaagggttg ataagcccca ctcgcgtcct gaacgaatat ggtcaaatac
     6781 tgagctgata ttatcctgct ggaaaatacc aaagacacta cgaacacctg cattagcaag
     6841 gcttgaaagt gggaaatcaa tcaaacgata tttcccacca aatggcaaac ttgctactgg
     6901 acggtggtcc gtcaatgtcg acatattgtg aaaaccaact gtatttccta aaatggcaga
     6961 atatttatca atcttcatct gttgctaccc ccactacttc attatatcct acaacttgta
     7021 cttcatctgt tccatcaatt tcgacaccgt cagaaataat cgcaccttca ccaataatgg
     7081 cacgtttaat cttagctcct tgaccaatga tagctccact catgataact gaatcaagga
     7141 cttccgctcc ttcgcgaact tgcgcgcctg ttgaaaggat agaatgttta acagttccat
     7201 caacgaaaca tccgtctaca actaatgagt cttccacatg agcatttgcc ccgaggaagt
     7261 ttggtggtga aatcaagttt cttgagtaaa tcttccattg acggttacga ctatccaagg
     7321 cattttctgg agaaatatac tccatgttcg cttcccaaag tgactcaata gtaccaacat
     7381 ctttccaata accactaaat tcgtaagcat aaacactttc acctaactca aggtaatttg
     7441 gaatgacatt tttaccaaag tctgacatgc caaccttgct cttttcagca gcgactaaca
     7501 tattacgaag gcgttgccaa tcaaaaatgt agattcccat agaagctttt gtagatttag
     7561 gttgagctgg tttttcttca aattcaacaa tacgattgtt agcatctgtg ttcatgatac
     7621 caaaacggct tgcttcttta agagggacgt ctaaaactgc tactgtcaag ctggcattat
     7681 tatccttatg agactggagc atatcatcat agtccatttt gtagatgtga tccccagaca
     7741 aaatcaagac atactcagga ttgacactgt cgatatagtc gatattttgg taaatagcgt
     7801 gactagtccc ctcaaaccaa cgatttcctt cacttgcaga ataaggttga agaatagaga
     7861 cacctgaatc aataccgtct agtccccagc ttgaaccatt cccaatatgg ttgttgagag
     7921 caagtggttg atactgtgta acgaccccaa cattgtgaat ccctgagttg gcacagtttg
     7981 atagggcaaa gtcaatgata cggtagcgcc caccaaattg cacagctggt tttgcgatgc
     8041 tttgagtgag tttagcgaga cgagttcctt gcccaccagc aagaatcaaa gctaacattt
     8101 catttttcat tttctactcc tttttggttt ttatttgtga cggttttagt agatttcaag
     8161 cgacgtttga ttttccatac acttgctccc atagccggta gggtaaaggt taaggtctgc
     8221 tcataatctt tccatagtcc ttcttgcgtt tgaacagttt gattatgttc tttccaaacg
     8281 cctccccact cttccaactc agtattccat acttcttcgt aaattcctgc aacgggtagt
     8341 ccgattgtaa aatctttccg ctcaacaggt accatattaa agatacagac taacatttct
     8401 ccctttttac ccttacgaat aaaggaaaga acactctggt ctcgattatc cgcatcaatg
     8461 atttcaatac catcatagct ggtatcaatt tcccacagac agcgatgatc tttgtaaaac
     8521 tggtttagct gagaagtgaa atacttcatc ttagcattca ttgggtcttc taggttagac
     8581 cattccaact gttcttcaga tttccattct aggaattgac cgtattcgct acccatgaag
     8641 agcaatttct taccagggtg acaaatttgg tacgtataga gattgcgcaa gcctgcgaat
     8701 tgattgtaac gatctcccca catcttatgc atcatactct tcttgccatg aaccacttca
     8761 tcgtgcgaga atggcaagag ataattctcc ttgaaaacat acataaagct gaaagtcacc
     8821 aggttaaagt catacttacg atagatcgga tcttcttcgt agaaacggag gatatcattc
     8881 atccagccca tgttccattt gtagtcaaat cctagaccac caatctcttt cattcccgta
     8941 atattggtcg cagacgaact ttcttctgca atcatcatca catctggata ttctaactta
     9001 ataacctcat tcaagcgctg aaggaaataa taaccttcat agttgagatt tccgccatct
     9061 ttattaggtg tccatggagc atcatcatag tccaaataga gcatgttgct aacagcatcc
     9121 acacgaatac catccaaatg atagacatca atccaatgct taatgcaaga aattaagaag
     9181 gactggactt cattttttcc aaggtcaaaa ttaagggcac cccaaccatg gttatgagcc
     9241 ttattatggt cttggtattc aaaagtcggt gtcccatcat aataggctaa ggcatcatca
     9301 ttgatggtaa agtgacctgg tacccagtcc acaataaccc caatattatg ggtatgacac
     9361 tcctcgacaa aatcttgaaa ctcctctggt ctaccatagg catgctctaa agcgaagtaa
     9421 cccataagct ggtaccccca actcaaacct agtgggtggg acatcaaggg cataaactca
     9481 atatgagtat agttcatctc aactaggtaa ggaatgagtt catctttcaa ttgggcaaaa
     9541 ctataaggac tgccatcagg atttctcttc caagagccag catgaacctc gtaaatattg
     9601 acaggacgtt cagcaaagcc ccaacgtttt cttcgtgcca gccaaagtcc atccctccat
     9661 ttcttatcag gaagctctgt tacgattgcc cctgttcctg gacgagcctc atacctgaca
     9721 gcaaaagggt caatcttcat cagttgatga ccattttgac gtgtgacatg atatttgtaa
     9781 atatgccctt cttgagccat attggtaaag acttcccaga ccccaaagtc atttcttacc
     9841 attggaatct gattttcaat ccaattggta aaatcaccaa ccaagtgaac agcctgagca
     9901 ttaggtgccc aaacacggaa ggtatagcca tactctccat ttagttcttc cctatgtgct
     9961 cctagataat gttggagata aaaattttca cccgtcataa aggtttttaa tgcttctcta
    10021 ttatccatat actccccttc tcctgtaagc gttttctatg tttttattat actacctttt
    10081 tagagaagat tcaagtaaat tactatactt ctttaattat tttgaaaatc tacaacaagt
    10141 tcacttactc gttcaattgt aaatcaatat tttttcaaaa aattgcgaaa acgcctttct
    10201 ttttctacta tagtgaaatg aaataaaaca tgcgcaaatc gattaaggaa tttaatctaa
    10261 tttctaacaa tgtcttagaa atcaaatagt aaaatgaaat aataacagga caaatcgatc
    10321 aggacagtca aatcgatttc taacaatgtt ttagaagtag aggtgtacta ttctggtttc
    10381 aatctattat aataccaaaa agttcgtatt ttttctataa ttattatttt atagcaaaaa
    10441 aataaaacca ggaaaacaat ttcctggttt tacacgttat ttcacatcaa aaacaatgga
    10501 tttgacattt gtcatcgctt caatgctata tttaattcct tgcactccag ctccagaacc
    10561 tttgacacca aggaatggga aattatctgg accacgctgg gttttattat taatgtggac
    10621 tgtacctact tcaagttttt cagcaatttc aaaggctttt ttgaaatcat ttgtaaagac
    10681 tgatgattga aggccgaatt cagattcgtt ggcaaaggca atagcttcct ctacactagc
    10741 cacacgaatg attggtaaaa caggaccaaa tggctcttcc catgccactt tcatatcttt
    10801 tgtaacttgg tcaaaaagca ctggccagag aagattgccc tcacgtttga ttggtgtaag
    10861 agcctgagct tctttttctt gtgcatcctc aatcaagctc caaatgaagt cggctgaagc
    10921 attgtcaata acaggtgtaa tatcagcatt gtcaaatgga tcaccaactg ttaatttaga
    10981 aacttcttcc tgaagcaaag tagctaattt atctgctaca ctttcgagaa caatgacacg
    11041 tttaatggcc gtgcaacgtt gtcctgagta gctaaaggct cccgcaacaa tttgcttggc
    11101 agcatgttcc aaatctgcat cttctagtac aagagctgca tctttcccac caagttccaa
    11161 catgatagga cgcataccag ctaaacgacc aatacgttct ccaataggag ttgaacctgt
    11221 aaagttgatg aagttgactt ctttgtgctc aatgatataa tccccaattt ctgaaccacg
    11281 acctgtaatg gtgttgaaaa cacctgccgg aatccctgct tcttcaaatg ctttagccaa
    11341 caagagtcca gaaatggaac cttgtgttgg tggcttaaac atgaccacat tccctgcaat
    11401 caaggcaggt gcaattttag aagcagataa attaactgga taattaaagg gagcaatcgc
    11461 tagcacgata ccaactggtt cacgacggac aacagccagt ttgtttttac ttgctgcctc
    11521 aaaaccacca ccttccattg cttgtccagt gatacggaga ccttcctcag cagcataacg
    11581 aatcaagtct gctgtacgca ctacttctcc aattgctgct ttaatccctt ttgctacttc
    11641 tttggcaagg atagtaccaa tttcttcctt atcgcgttct aaaatagctg ctgttttatg
    11701 taaataagcc gcacgttcaa ctgctgataa agctcgccat gctggcaggg ctgcacgcgc
    11761 agcttgcata gcctcatcag cttcagtctg agtcatggct ggaactgtac ccaattcttc
    11821 ttgattgatt ggtgaataaa tcgtaatttc ttgttcagat gatttccatt ttccatttac
    11881 taaattctga tatcttgtca aattcttgtc ctcctgaaaa tgattaagat atattttacc
    11941 aaattttcag aaaataacaa ccctttttgc aaaatttttg agattatttt cacaagcttg
    12001 atttttcaaa gtatactcaa taaaaataaa aaaatccact acgtcaaggc gaggctaatg
    12061 tggtttgaag aaattttcga agagcgtgaa tgagtatcat ctatagtaaa ataaaaaaac
    12121 tgaacaattt ggttggggac agccaaacca atttctcaca atgttttaga aacaagggtg
    12181 tgctattcca atttcagcct actataactg tcatagattg ctgaaacaaa gtctaggtaa
    12241 aagtcttcat aataaaaaga cctcctatca aatgttcaaa aactttgata ggaggtcttg
    12301 tttcgtgaaa atatttatca aattttctat acaagtgagc tgttagccag gttctttcta
    12361 ttctttcaat ttcaatgaat ggatttttta ccaatactca taactgggaa tttttctgtc
    12421 taaaaatagc gagatagatg gtatttataa aacactcaag acagctagac taatatcatt
    12481 taaaacatta tcttcttttg agcgactgtt ggttaccaac atagctaaat ttcctgcatt
    12541 ttcaaattga tagggttctg atttagcatt cacaactacc aagaggtgtt ctttgccgtg
    12601 aacttcatag ataaggcagc cgctatgttc aatcgcagaa tgcacaaaga catgatggta
    12661 aatttcatca tagctagagt aagaaaaggc accagttttt gtcttcaatc ggatgacttg
    12721 acggataaac tcaatactgt cttgacgctc attaatcaag ttccagttca cttggttcac
    12781 actgtcagga gcattatagc tattcatcgc acgctctcta tcatcatggg tcaactcacc
    12841 attttcacca gtcgcaacca gtttggtacg accaaattct tgaccgattt ccataaaggc
    12901 catcccctgc atgagcagat tcatggctgt ggcagtttcg accttgcgca tgatttgctc
    12961 tgaactttgg tctggatgaa gggttgccaa taaatcgtga agattgtaat tgtcatgggc
    13021 ttctacatag ttaagcacct gatttggatg tgtatagctt cctaattcac gacttcctag
    13081 gattgcttta gctagaattg gctctgtcgc agcaccactg acaaaacctg acttgatagc
    13141 accataaact tctccccctt tgacagcatc gcgctgattg tcattaaaga aaccaatatt
    13201 tggcatctgg taggcattgt ccttcttggc cttatcataa ggggcaagac ctgttcccat
    13261 atcccatcct tctccataga ggataatgtt ggagtcgatt tcatccaagc tttgacgaat
    13321 catctgcatg gtcttgacat catgaatccc catcaagtca aaacggaagc cgtcaatatt
    13381 atattcctgc acccagtata gaagagaatc aatcatatac ttgcgaaaca tttcgtgttc
    13441 actggctgtt tcatttccaa cacccgttcc attctggaag gtaccatctg gattcatacg
    13501 ataatagtaa tcagggactg ttgtttggaa tggtgcatca acaactgaga aggtatggtt
    13561 atagactaca tccataatga ctccaatacc cgcatcgtga taagcttgaa ccatcacctt
    13621 caaatcacga atgacctgag ctggatcatc tggattagtt gaaaaactag tttctggcgc
    13681 gttatagttt tgtggatcat aaccccagtt gtaggttaca tttccatcct catcgtattc
    13741 tttatgacgg tctgcaattg gttgcaattg aacataattg tagcccagct tcttgatgta
    13801 atcaaaagca gttgactggc cgtattggtt aactgttcca gcctgagcag cacccaagaa
    13861 agttcctcga agatgttcat ctacacccga tgtaggtgat ttagtcaaat cacgaatgtg
    13921 catttcacag ataactgcct tacatggatt ttccaagcgc caagtagcct ccgaaccgtg
    13981 cttaacctcg aagttttcaa cttgcttttc tacatggctc agaatagctg aacgtttgcc
    14041 atcagggctg gtcgcgattg tataaggatc acgtgtcagt gtttggtgat gagggaattg
    14101 gacttgatac tgataagtct tacctaccaa attttcttca acatccaaac tccagacacc
    14161 gattgtattg tccttatgat tataagagta gctattgcct cttttcatct caaaagtctt
    14221 ccaaacgggt gcatcattag cagctgattc ataaacgaca acttgcactt ctgtcgctgt
    14281 aggtgaccag agagaaaaat gagcctgatt gtcctctaca cggcaaccca attctccttg
    14341 gtaaccccaa tgatgatcaa aactagcact gttaatggcc ttatcaaagg caaaaggatt
    14401 ttgattttta tagaaaggac tggcaatagc aggattttca gagtaataaa tcctatcatc
    14461 gccttccaaa atccagacct ctgttaatag gggatagtga ttaaaacgga tagaatattc
    14521 tttactagtt tgacctgtat gaaccacaaa attcaagctt tctataacat gtgaacttgg
    14581 gtgttcaaag ctaaataaag ctccaaaata atcttctttg taggttagca aatcaattcg
    14641 ttgatcctga ctttttacaa aggagcaagt gtcatattct ccattcttac gatggtaatg
    14701 aatgcgcata gggtagttat acatttttta tttttccttt ttactttgtt tctatttcac
    14761 taataaattt ttgtcaatct cgtctcaatt aacagacata gtcatattct ctaaactctg
    14821 tttttaaacg atccattaca aactttctag ccatgcctca tctctgacct ggataccaag
    14881 ttcttgtgct ttttgcagtt tacttccagc gtctgcacct accacgacga ggtcggtctt
    14941 tttagaaata ctacctgtca ctttggcacc cagactttcg agtttacttt tagcttctga
    15001 gcgcttgagt cgttccaatt ttcctgtcaa taccacggtc aaacctgaca aggccgcatc
    15061 cgctactacc gtctgtcctt tatagtccag attgacccca gtttctttca attctctgag
    15121 cagaatttca gagccttctg tcgcaaaata agtctgaaga cttttggcaa tcacgccacc
    15181 tagactttca atactagcca cttcctctga atctgcctga gacagatttt caattgaatg
    15241 gaaatattga agtaaaagct gactaacctt gcttccgaca tgacgaattc ccaaaccaaa
    15301 taagagcttc tcggcagaat tttcctttga tgcttggata gcctgataca gtttagcagc
    15361 ggacttttcc ttaaccccct ctaaaaggag gaaatcctct tcttgcaaac gataaatatc
    15421 cgccacatcc ttgactaaat tagcagcaaa aagcttctca acaatagatg gaccaaggcc
    15481 tgtaatattc atagcatcac gagaagcaaa gtgaatcaag ccttccatga tttgagcagg
    15541 gcaacgcgga ttgatacaac gtagggccac ttcatcttca aagtgcaaca agtcagagtt
    15601 acaacttgga cagtttgtag ggatatctag tttttcttca gagacccgtt tggactctac
    15661 cacacgtaaa acggcaggga tgatgtcacc agccttatat acaatgaccg tatcgtcttt
    15721 tcggatatct ttttcagcaa tataatctac attgtgcagg gtcgcacggc taacagtcgt
    15781 accggcaagt tgtactggtg ttagattagc agttggagtt acaacaccgg tacggccaac
    15841 tgtccagtca actgataaga gttgagcttc tttttcttcg gcagggaact tgtaggctac
    15901 tgcccacttt ggagccttaa ctgtaaaacc aagttcttct tgacttgcta ggtcgttgac
    15961 cttgattacc actccatcaa tatcgtaagg cagattttcc cgttcctgtc ctacttcttg
    16021 gataaaattc cagatttcat ctatgttttc agccaagatt cgcttaggat tgaccacaaa
    16081 acctagttgt tctaggtact tcaaaccctt ttcttggcta tcacgagttg aagggctggc
    16141 ttcttgatag agaaacgttg caagattacg cttggcaact actgctgtat ccaactgacg
    16201 cagagttcct gctgccgcat tacgaggatt agcaaattca ggctctccat tttcttggcg
    16261 cacttggtta acttggtcaa aggaagcgcg tggcatgtaa cattccccac gaactgtgat
    16321 atctagttct tctggcaaag tcaaagggat gtccttaaca cgcttgaggt tttctgtgat
    16381 attttcacca attgaaccat ctccacgtgt taccccagca accaaaatcc ccttttcata
    16441 agtcagcgag atagataagc catcgatttt cagctcacaa atataggtcg gatgagccac
    16501 ttccttacga acacgcgcat caaaagcatc tagctcctca cgtgaaaaag catcctgcaa
    16561 actataaaga ggatactgat gactgtattt ttcaaaacca tctaaaacct tgccaccaac
    16621 acgatgagtc ggactgtctg ctagcacttg ctctggataa gcagtttcta actcgaccaa
    16681 ctcacggtaa aggcggtcat actcactgtc tgaaaccgag ggattatcgc tggtatagta
    16741 ctcagtcgca tagcgattga gcaaagcgac taactcattc attcttttat tcataagacc
    16801 attttaccat aaaacaagcc ctcctcacaa acgagaaggg cggaaaaaac acttagtttg
    16861 aaattatttt tgaaactcaa gcaaccttat atcaattttt caaaatgagt tcgaacataa
    16921 ataaacgata tacaagacaa gatgataaca ccacttccaa ttatcaggaa agaagagaga
    16981 tgtacacttg gcaagactgt cataaatcct tttgcaatag gcataaatag aatagctaag
    17041 gtaaaaattg tactcagtac tcttccaaga aattcgctct caaccttggt ttgtacttga
    17101 gtaaaaaagt gaatattaaa aatcgtcata aacaattcac aaactaaatt tccagaaaag
    17161 gaaagaaaag ttggaagtgg taatcccatc ataaaaactc cgacacctgt caaagccagt
    17221 aaaatcaaaa gattataaat attagcttta attttactag ctagaagagc cccaatgatg
    17281 gaaccaatag cccccatagt taaaatactt gcataggctc cttctgaccc gtaaagctga
    17341 ttcgaaaagg gaagtagaaa ttcaaaagct gcaaaaaaga aattaacgct ggaagctacc
    17401 agcaaaagga agaaaatttc ttgctgatgc cagatatagt gtaacccatc cttgatatct
    17461 acaaaaatat ctctcccagt aaaagccttt ttctcttgaa cttctgcttc ctcttttgga
    17521 aggaaagcca ctagaacaaa agcaatgaaa aaagtcagcg agtctagcag tagcgtcata
    17581 tggagacttg caaactgtaa aacaaggaag gaaagaacag gagagctaac acctacaacc
    17641 tgcaaaacca gctctaggcg agaattatag atcacaatct catctttctc caccacttca
    17701 gttatgatag ctttattggc tgtgcgagaa aaggcaaaag caatagcctg cacaatgtta
    17761 gcaacaatca aagcgccaat catccagcta tcattcctta tgaaagaaat agccagacaa
    17821 agaatcccac aaacaagatc tgccgtcatt aaaatcttac gacgagaaaa acggtctgaa
    17881 ataactccgc caaagggatt gacgagaata gatgtgacga gctcagaaat ctgatacatt
    17941 cctaaaactg tctgtcctat agtccccata gaagccaacc agacactatt tccataatca
    18001 tagagcatat ttcccatttt attgatagcc ccacggctaa tcaactgcac tgcatagcga
    18061 ttcatattaa agctcctctc aaattttgaa actattgtat caaaaccgaa aggagctttt
    18121 tattttttcc cttatttggg aaaattaact tttgacaaat ttttcgtagt gttcctgata
    18181 ataggctact tgctctggaa gacctaacac atcaaaaata tgcatggcct cttgcatctg
    18241 cttacagcct tctttacact gtcctttttg atataaggca aaacctttta aataatggaa
    18301 aacattacgc tcataaagct taataccttt gtcaataatc ttctctgtat aagcctcaaa
    18361 atagttggca ttataaaaag aagaatgctc taaacaatgc tggtaacaat tgagggccaa
    18421 aatcaacact aatctcttat ggcgactaat ctcttggtaa aattcctccc tctccataac
    18481 ttctctacca atccgagtga catagtctac atcgtagaaa ctatagaggt taccgaaaag
    18541 aatcaactca tacatggtcc attcttctgt tttgaagaga taatctgcta ccttatccaa
    18601 atcatcctgc ttcatatcat aactcgcatc tctttgacaa atcagacctt gtagcaaaat
    18661 ccagttcagc tcaaaataaa ggggagtcgt cgaactctta gacttttcaa gttgttctct
    18721 ttgaagcttt tgaaaacctg caatatcgtt tgaatagtaa agtgggataa tctgtgccat
    18781 catagacaca tgttcatgat tatgaaaatt ccttgcctta tccatgaaat tttcgattgt
    18841 tacatgaatg ttatccaaaa tctcaaagaa acgggagact gccaggtcag actccccaag
    18901 ctcaaagcga gataactgag aggtagagca ggattcgcct gctgcttcct ttaaagaata
    18961 atttccactt gttcgaaatt cacgaaatac ttttccaaga tgttccatct ttacacctgc
    19021 tctgataatt cttcccactc aagcatagct tcttcctgac gatggctgat tttgtccagc
    19081 tcagcctgta attccatgag tttgtcggca tcgtttgttt ccaacatttg ttcagaaatg
    19141 gcttggcttt gactttctag ctcttcaatt tcagcttcta gactttcgat ttgtcgcatg
    19201 agtttgcgaa cttctttttg actttctttc tgggcctgat agtcattgac tggacttgct
    19261 tcctttgctt gattgctagt tgaagcttcc tcagtctgac tcatttctgc tgttgctttc
    19321 ttctcaacat agtagtcgta atctccaagg tagagagttg aaccattctc agacaattcc
    19381 aaaacatgag ttgccacacg attgataaag taacgatcat gactgacaaa aagcaaggta
    19441 ccatcaaagt caatcaaggc attttctagc acttccttac tatcaatatc caagtgattg
    19501 gtcggctcat ccagaatcaa aaagttattg ttttccatag aaaatttagc taaaagcaaa
    19561 cgagcttttt cgccaccaga tagcatgccg actgattttt taacatcatc tcctgagaaa
    19621 aggaaggctc caagacggtt gcggatttca acttctggtg tcagtttgaa atcattccag
    19681 agttcatcca gcaccgtatt acttggtgtc agcttgcttt gggtttggtc atagtaacca
    19741 acctcaacat tagcgccaaa gcgcttttct cccttgataa aaggaatctg gtccacaata
    19801 gacttgataa aggttgactt gccgatacca tttggaccaa cgatagcgac agcattcatc
    19861 ttacgaagat ctaggttgat aggttgtgac aagacttccc cgtcatagcc aacagctgca
    19921 ttttcaacag tcaaaacaac attgcccgac gttttttcag actggaaggt catgttggct
    19981 gctttcttgc cagcttcagg cttgtccaaa cgctccattt tttctagttg tttacggcga
    20041 gattgagcac gtttagtcgt tgaagcacga actagattgc gattgacaaa gtcttccaga
    20101 gcagcgattt ccttctgttg cttttcatag ttttttgcct cagtagctag cttttgctcc
    20161 ttcaactcaa caaaacgaga gtaattcccc acatagcgat ccaaggaatg cttggtcaaa
    20221 tctagcgtaa ttgtcgcaac cttgtccaag aaataacggt cgtggctgac gataatgagg
    20281 gcaccgctat agtttaccaa gtaattctct agccaggaga tggtttcaat atccaagtgg
    20341 ttagttggct cgtccaagac caagagattg ggcttttcaa ggagcatttt agctagagcc
    20401 aaacgagtat tttgaccacc agaaagctca gcaattttca tctgccacat agactcgtca
    20461 aacttgaatc cattcaaaat cgctcgaata tcagcttcat aggtaaagcc acctgcttgg
    20521 cgaaaatttt cagataagcg gtcataatct gacatcagtt tatccaaatc ctcaccagac
    20581 ttttcaccca tctccagctc catctgacgc agttgtttat ctgtccgacg taagtcatca
    20641 aagacatgaa gcatctcatc atagatggta ttttcagact caaaacggct atcttgagct
    20701 aggtaagaca gagaaatatc ttttttctta ttgatttctc cactagttgg ctcctcttct
    20761 ccaactaaaa tcttcaaaag agtagactta cctgcaccat ttttcccaac aagggcaatc
    20821 cgatctcgtt catcaacctg caggttgata ttatcgaaaa gaacctctcc tgcaaaagaa
    20881 cgttcaattt tattagcttg taaaataatc atacaagtag tatagcatgt ttccctaagg
    20941 cattcaagat aatcgtaagt cttttagtac aacttttata acataaaata aactaaatta
    21001 tgtatatttt atattagatt ccttcactat cttgttggat tttctaacca gctaatcttg
    21061 tttcaaatag ttatcgcaca agcctattat ttaattcttt tcattattta cgtacgcgta
    21121 tagcagattg aaataagatg agaacaaatc gattgggaaa gtaaaattaa tttctataaa
    21181 tgttttagca attgtttcgt actattttag attcagtcta ctatatatac aatattttcg
    21241 gaacattcaa ctttttaact ctatttatta ctagatttca taattaaaaa gcctactgac
    21301 caagctagaa agcttgatac aataggcttt ttaaagactg attatttaac agcgtcttta
    21361 agagctttac cagctttgaa tgctggtact ttagaagctg caattgtcat ttctttacca
    21421 gtttgtgggt tgcgaccttt acgttctgcg cgctcacgaa cttcaaagtt accaaaaccg
    21481 atcaactgaa ctttttcacc agctgcaaga tagtcagcta ctgctgcaaa tacagcttca
    21541 actgctgctg ctgagtcttt cttagttaat tctgtagctt ctgctacttt agcgatcaaa
    21601 tcttgtttgt ttgccatgtt aatgattcct ccaaataatt tctaattaac aaatataatc
    21661 atatcctaaa atccctttta ggtcaagtta aaaacgctat ttcttcccat tttatttatt
    21721 tttttaggag tggtaacgta tcaaaatagc ccaagcgttc tcacccgtgt gagtttgaat
    21781 aatggaaccc gtttccaaaa cagaaattgg cttttcaaca taagcttgta agctttcttt
    21841 catctctttt gcccaatcat cactaccaga atatgaaatt ccaatctctg ctacagcacg
    21901 ttcagaaagc gatgttatca actcatctaa ccatttttta aatgttttag ttccacgacc
    21961 tttaaccatt ggctgcaatt catggtcttt catttgcatg acaacacgga tattgagaag
    22021 agagctcaac aagccagtta cacggctaat tcgtccacct ttgacaagat tttccaaagt
    22081 tgaaacacca atataaagct ctgtatggtt tttaacctct tctacatgag ataaaattgc
    22141 ctccatatct ttaccttctt gagctaactt cgcagcctca acaacttgga atttcagggc
    22201 ttggtcagtg aaggaactat caacaacagt cacatctgca gtagataggc tagcaccttg
    22261 gcgtgctgct tctaccgtac ccgaaagagc atgggacata tgaatagcaa gaatctggcc
    22321 accatctttg cataggtctt caaaaatctc agcaaagaca cctacaggtg gctgacttgt
    22381 tttcggaaga ttcttacttt cttgcatcaa ctgaagaaat ttaccttctt ctttcaaatc
    22441 cgcatcagaa taaacaacat tatcaatcat tacagataat ggaacaattg taatatctaa
    22501 ttgctttact agttcaggtt caatagtaac agatgaatcg gttacaatct taatttttgt
    22561 catagtatca atctttctat tttaggattc agattggttt ccttacttct aattatatca
    22621 aaaaaaagat taaaaatcct aatggagtca atcaaatttt ccgtaaaatt tgatataatc
    22681 aacttataag aaaagaggtg tcctatgatt aaaaaaattt accccatttt taccatttta
    22741 ctaggtgctg ctatttatgc ttttggactg acttattttg tagttcccca tcatctcttt
    22801 gaaggagggg cgacaggcat taccctcatc accttttatc tttttaaaat ccctgtttcc
    22861 ctcatgaacc tgctgattaa tattcccctt ttcatcctag cttggaagat ctttggagcc
    22921 aaatccctct attctagttt actaggaacc ttagctttgt ccgcctggtt agcttttttt
    22981 gagcatattc cccttcatat tgatcttcaa ggtgatttac taatcacagc ccttatagcg
    23041 ggaatcctat tgggaattgg ccttggaatt atttttaatg ctggaggtac aactggcgga
    23101 actgatattc tagctcgtat tctcaacaaa tacactcata tatccatagg aaaactgctc
    23161 tttatcttag atttttgtat tctcatgttg attctcctaa tcttcaagga tttgagattg
    23221 gtttcctaca cgcttttgtt tgattttatt gtttctcgtg ttattgattt gattggtgaa
    23281 ggaggatatg ccggcaaagg ctttatgatt atcacaaaac gtcctgacca acttgctaag
    23341 gcgattaatg atgacctcgg aagaggtgtt acttttattt ctggtcaagg ctactatagt
    23401 aaagaaaatt tgaaaatcat ctactgtatt gtcggaagaa atgaaattgt gaaaacgaag
    23461 gaaatgattc atcgaatcga tcctcaagcc tttataacta ttacagaagc ccatgaaatc
    23521 ctaggagaag gcttcacctt tgaaaaagaa taaaaagagg taatgtcgtg acctcaaaag
    23581 ttagactaaa tcatctatct tttgggttac agacaacctc ttttttgttt tatttactca
    23641 agctcttaag accaattccg agttacttct tcatcagcct ttaactgatc cactaattgg
    23701 tcaactgagt caaatttggt catatcacga atgcgatcaa gccaataaac catgacggtt
    23761 tccccataaa tatcttgatt aaaatcaaaa atattgactt caaaacgtgc ttcttctcca
    23821 tcaaaggtca catttttccc gacactagcc atagcacgat acttctgtct ttgaatctca
    23881 acatcaacga cataaacgcc atctgctggc atataagtac ggtctaaaag cactaaattc
    23941 gctgtcggat aaccaattgt acgaccacga gcattaccat gaactaccat acctcttgat
    24001 ggaagcggtg cccccaaaag ttttcctgct tctttcacat ttccatctaa aatagcttga
    24061 cggatacgag ttgaactaat ctttcctttc tcatcttcta caggtggaac aatgataact
    24121 tctccatcaa agtaatcctt taaatcttct gctgtttttt tgtcagaacc aaatgtataa
    24181 tcaaaacctg caacaataat tttggcattc atagccttga tataagttgc aaagaattct
    24241 tgtgcagtga gactagcgaa ttgactacta aaatcaagga gatataattc ttctacacct
    24301 tcgcgcttta attttctttc acgttcagca gggttcaaaa tatgcaaaaa caaatctgga
    24361 tgataaggct ctaaagcgat ctttggagat tcattaaagg tcataacgac gataggcaat
    24421 aaatcctttc tcgcagcctt gttggcaaca cgaaataatt cttgatgccc cttatgtatg
    24481 ccatcaaaat agccgagaac aacgactgaa tcagatggtg tgccaatatc tttttggttt
    24541 tttataggaa tagtaataat cataaaataa ttatatcata gcgatagcta tttctggaac
    24601 agaaaatctg aaatgttgtt tttttcacat gaagtgtacc tgttttcaaa aagcacttta
    24661 ttctatcgtt gcttaactat gaactttgca atattcttct caaaaacttg taggacatct
    24721 tcaaaatttt gcaaggagtg attagacttg ttcggtaact ataaagtgtc atactatgct
    24781 tatgtatgaa aaagcaatgc aactaactcc tgagaacttt aaattactaa ttggtgccga
    24841 aaaggtagaa tttagaatag aggtacacct atggctgtaa aatttacaaa atgagacaac
    24901 ttgggcaaga tgtttgaaga atttcctaaa ctccctgatt tgaagcaagt cactttccct
    24961 gatgacaaag aaaaaagcca aagcaataaa gaaaaactag atgactgctt tccaacaact
    25021 cccatctagt gtgcttcaga ctgggctatt tttctctcca tctgttagct tggattctca
    25081 gaccgtttca gctaaagaat atcttttcct ttatcagaag gaacggctca agccattcag
    25141 acaagtgaag ggacgacaag ccaatatttg aaaccagata gcagttctta tagtcaattg
    25201 aaataaaatc tgaagaaatc gagtaggaaa ctcaaatcaa tttttagcag tgttctattc
    25261 cagattcata ctcaatgaaa attaaagtgc aaactaggaa gttagccgca ggctgtactt
    25321 gagtacggca aggtgaaact gacgtggttt gaatttgatt ttcgaagagt atcaatccac
    25381 tatacatatt aaaaagtatg ggtatcaaaa taaaaaaaca ggctctccga aaactcggaa
    25441 agccttattt taagctgttt ctagtttcct cacctttaca tttcaaggtt cgggataaaa
    25501 ggttcaatgg acctttttat ttagcgattg ggtaaacaga aacttgtttt ttatcgcgtc
    25561 ctttacgttc aaagcgtact acgccttcaa ctttagcgaa caaagtatcg tctccaccac
    25621 gaccaacgtt tacacctgga tagatgtgtg taccacgttg acggtaaagg attgatccac
    25681 ctgttacagt ttgtccgtca gctgctttag ctccaagacg ttttgcttgt gaatcacgtc
    25741 cgtttgatgt agaacctcca ccttttttgt gggcgaaaag ttgcaagttg ttaagagtca
    25801 tttttaacat aatgttttcc tccgtgttag ttttctgtga taactctggt ttggacgaac
    25861 tcagaagagt tctccgataa gtttgccata cctaagaaaa atgattcaaa gaataactgg
    25921 gtcatttctc tctggtgtga aggaagatct tttggtattt caaccatcag atagccacct
    25981 tcatcttcgt ttaattctag gattggttca tagcctgcaa atttctcaat agaattgata
    26041 aagttaatgg caagcgtaga aaccgatgca cacacgacat ctaagccgta ttcgccactc
    26101 tcggcgtgtc cagtaatttc cgcactcctc agctcgccat cttcggctct ctcaaagact
    26161 gcctgtatca tgtgttctcc ttaaaattaa gcgttgattg cgttgatgac aacttttgta
    26221 tatggttgac ggtgaccttg tttacggtgg ctaccttttt taggtttgta cttgtaagta
    26281 accactttct tttgttttcc ttgtttttca acagttccaa ctacagtagc tccagcaaca
    26341 agtggagttc cgacaacagt gttttcacca ccaacaagaa caacttcgtt aaaagtaact
    26401 tcttgaccag cttcaacgtt caatttttca acgtaaactg cttgaccaac ttcaacttta
    26461 acttgttttc cgccagtttt gataattgcg tatgtgctca ttatgcacct cctatgattt
    26521 ttatgggttt ccccgatttt ttgtgaagac tcgcctagca tcgtgggacg aaccacttaa
    26581 acttgaataa tcaagcaacg atgttcgtgc ggttgcacag gatcgtgcat agtcaactct
    26641 tcaagtatag catatctcct attttcttac aagtaataac acctaaaatc aagctttttc
    26701 ttttactttt ttctgccaag aggcaaaaag catgctgagg taaaaaacgc tcatcataat
    26761 aggaacacca agaatggtct tttcatgata gaaaatcgtc aaataggctg aaaagacaac
    26821 gccaaggaca aaactactaa gcaggctaac aaatatgaat ccttcacgca aaaaaggagt
    26881 gtgattggtt cggaaataat ctccaaaagc cagcatggtc cgtttgatat tccctgtcat
    26941 aaaagcgtta ttataggcaa tacccgacac ttctccaaaa gcagttgtca ccagtcccat
    27001 acagaaggcc aagggcggca ctagatagat attatccaca gtttgcggca caaaagcaat
    27061 aatgattgat aagattgcca agggaatcaa ggacagaata ggttttttca caattctcaa
    27121 tttttcctta taaatcgtta ataaaaagac tcccatcata aacgctagca aggtgagaac
    27181 cttgtcccta acatccgaaa cattattttt aattaattct actgaaagaa agacaacatt
    27241 tccagtttgt ccagctacaa gggtattccc gcgaacaata aaagtgtaag catccacata
    27301 tccagcacaa aacgtcaaaa aaagtgctaa ccttttagac tgacgtgata tttttcttat
    27361 aggtaataac ctcattttac ctcccattgt attttctctt agaaatattg taccattttc
    27421 tttctaaaaa atcgtaggct accatttaga ttttactatt agcataaaaa taataataga
    27481 caactattta tccaaaaata gatagatgta acatgtttgc aaacaaagca tacgaacctt
    27541 tagtaaaatc atttccatga aactagaata gagccctctt agcaaaaatc attattttaa
    27601 tttatttcta atcactcctt gacataaata actctcacca ataaaagact atgtcttaaa
    27661 aaaatggtat aataaaatca atacttgggc ttgatggcta tgctactaat aacaattagg
    27721 agagaaaatc aggcacttgt taacaacaag gattatcccc ttgagatgaa aggaacttta
    27781 gaaatcttat gatgaacatg caaaacatga tgcgtcaagc acaaaaactt caaaaacaaa
    27841 tggaacaaag ccaagctgaa cttgctgcta tgcaatttgt tggcaaatct gctcaagatc
    27901 ttgtccaagc gaccttaact ggcgataaga aagttgtcag cattgatttc aatccagctg
    27961 tcgttgaccc agaggacctt gagactcttt ctgatatgac cgttcaagcc atcaactctg
    28021 ctcttgaaca aatcgatgaa actaccaaga aaaaactggg tgctttcgct gggaaattac
    28081 ctttctaaaa acaaggagct agaacaatgc ttgtcgataa caaaggctaa gaaaggtgca
    28141 aaaattataa aaaacgcata atagcaggtg tcaaagcact caatattatg cgttttctca
    28201 tgaaaaattc tcttttcata ctcatttaaa atcaaaaagg gaatttatcg ttttacggac
    28261 atgtatatta cagatttcta taggtcgtgt atagacagga tatcatcgca tggaagtttt
    28321 cctttttgac accaatcagt aatttagttc tcaggaatta atggcagtgc ttttcgtaca
    28381 tagtttcata actacagtat agcatagtat atcactttat agttttcact attgatgaac
    28441 tgcttgagct gctgtgatga gggtcaactt gtaaacatcg tctgcattac atccacgaga
    28501 aaggtcgtta actggtttgt tcaaaccttg caaaacaggt cctacagccg caaaaccacc
    28561 aagacgttca gccatcttgt aaccaatatt tcctgcctcg atacctggga agatgaagac
    28621 atttgcttga ccagctaccg tacttccagg agctttcaga gctgcagttt caggaacaaa
    28681 ggctgcatca aattgcaact caccatcgat ttcaaggtca ggacgcaagt cgtgagcaat
    28741 tttagttgct tcaacgacct tatcaacgct ttcaccaaac cctgaacctt tagtagaata
    28801 gctcaacatg gcaattttag gttcgatgcc aaacatctta gctgtgattg ctgagttgat
    28861 ggcaatttca gccaaggctt ctgcatctgg attgatgtta atggcacagt ctccaaatag
    28921 gtaacgttcc gtaccacgaa ccatgaggaa ggctcctgaa gtacgagtta catttggacg
    28981 agttttgatg atttgtagag ctggacgaac tgttgaagct gttgagtgaa tcgctcctga
    29041 caccattcca tcaaccaagc ccaagtaaac caacatcaca ccaaagtagt tgacatcttc
    29101 aaccaaaacc ttgcgtacat cttcttcagt cattttgccc ttgcgacgct ccaccaaggc
    29161 agaaaccatt tcttcaaatt gaggataatg ttgagggtcg atgacctcat aaccatccat
    29221 gattccttca atttcaagat aaattttaat tttttcagga tttccaagca aaacaggaat
    29281 cacttctgtt tcttttacta agcgttttgt tgcttgaaga atacgaggct cttccccttc
    29341 agggagaacg atacgagcat ttttaccaac aaggttggct ttgagacttt caaaaacttc
    29401 catgagtttt ctcctttaag ataataatta tactctgaga cagtttttat agagtattag
    29461 ttgataactg ggtaataagg ttactgaaat catccggcaa gggactttct aactgcaagt
    29521 cttgctctaa aaatggatga taaaaggata ggtaatggca atgcagagcc tgacgttgaa
    29581 taccatcttc cagactacca ccatacaaat catctcccag caaaggaaaa ccgatatgag
    29641 aaaaatggac tcggatttga tgggttcgac cagtgtgcag gtgaatatag accaagtgaa
    29701 tatttccata agaagctaca atcttgtatg aagtatgggc atactttccg cctttagcca
    29761 ctcgtctggt aataatggaa tcttcatcac gcgcaatcgg agcaataatt tccccttctg
    29821 gctccaaatg tccatctccc ttaaccaaag caaagtagcg tttctcgata gatttcttct
    29881 gcaactgctt gtctaatcgt gcatgggcat aaccgtgctt ggcaaagagc atcaagccag
    29941 aagtgtccct atctagtctg gtaacaatgt gaacctgctg attttcataa ttttgcttga
    30001 cataataacc cttgataaaa ttggcaatgg tattagagtg attgacacta ggaatagaag
    30061 ccactccata gggtttattc aagactagaa agtggtcatc ctcatagaga atatctaatg
    30121 gaagctcaat agcctccaag gtttcaaagc ctttctcagc gggaatgtca atggtaacgt
    30181 agtctccaac gtccaataga tacgttgcat tttgcggttg attattgacc agaatagctc
    30241 cacctcgaaa cttaatcttg gccagcaatc ccttagaaac ctcgtgcttt tttaagaagg
    30301 tcttaacctt gacatgttca tctgcgataa attcaaacct cattcatcca cctcaccgat
    30361 aaaggcatcc ttaacacggt tccagaaact ggtatggcta ggagtcgcga caaagtgaat
    30421 cttatgatgg tcgatttgat actcaatacg ctcaatatta cggaaagaat aaacgctatt
    30481 gtcaaccgaa atagtatgat aatcgtttct tgttggaata agttcaatct tatccttctt
    30541 aggcacaata atggaagagc ccagtgttcg ataaacacga ttattaaggc tggcaatttc
    30601 cgttaattgc aaagcttcaa tggtagggtg taaaacagca ccgccaagag acttgttata
    30661 ggcagtacta ccagtcggtg tcgaaactgt tagcccgtct ccacgaaaac gttcaaaggg
    30721 aacaccattt attacaatat ctgccaccat ggttcgatca gacctgcgga tgctggcttc
    30781 gttgagtgct ctgaaaatct taacttcacc attttcaaga aagaccttca cattcagaac
    30841 agggtaagaa acccttgccc cagtatctag ctgcaaatta gtcactagct tgtccaactc
    30901 aaaatcacga taatctgtat agaagcccaa atgtccagta tgaagaccga taaagcggac
    30961 cttgtcaagc tgattttcgt acttatgaaa ggccgacaag agcataccat ccccgccaat
    31021 ggaaatgaca atatccggat tggtatcatt gagtataaac tgatttctct tcaaacgatc
    31081 tcgcaattca tacaaaaccc tttgactctg cggttttcta ttggctatca gatcaattcg
    31141 tttacctgta ttcttcatct gtatcgtcac tgtttcctac accgtcattt aattttctac
    31201 tcaaaggatc aaaaagtgcc tgagcttctt ggatatcatc acgaatttca cccatttctt
    31261 catccaactg atgggcgatt ctagctgtaa tttccagtcg cttcttaatc tcatctggga
    31321 aatccccttg gtacttgtag ttgagagaat gttctatcgt tgcccagaaa ttcatggcca
    31381 aagtacgaat ttgaatttct gccaaaatag tcttagctcc attgatggta tcaaccgtat
    31441 attctactac cacatgatag gaacgatagc ctgatgcttt tctatgagta atgtaatctc
    31501 gctcctgtat gattcgcata tcctgacgct tgtgcaaaat atccactact tccttgacgt
    31561 catctacaaa ctgaaccatc acacgtaagc cagcaatatc ctgcaaatcg tgttccaagg
    31621 tcgcataagt aatgccacga cgagccattt tttctttgat gctctcaatt ggcttgactc
    31681 gaccggtcac aaactcaatt ggagaatgct tattttgctt acgatattgc ttacgaatac
    31741 cacgaagttt aatctttaac tcaccaacag cttgaatgta aggatctaga aattcttccc
    31801 attctaaggt catatattct cccttgttct catattatcc gtcaaaaata tctttgattt
    31861 tttctccaga ttcatataca ataaatacaa tgcttattat accataaatc cgctttcaac
    31921 gataaaaaaa ggtaagaaaa atgaaacatt tagaaattga attgaaaaca ctattgaaaa
    31981 aagatgaata caatcgtcta aaagaccagt tcacaggtgt cactcctgtt cttcaaacaa
    32041 attactacat cgacacgcct gattttgaac tgcgagaaaa gaaagtcgct atgcgcattc
    32101 gaacctttga agactgggct gagttgacac tcaaagtccc gcagagtgtt ggaaacatgg
    32161 aatacaacca aaaattgcaa ctaaaagatg ctgagaacta tctgagtaag gaagaacttc
    32221 ctcaagggct agtactggat gaattggcga aacatggtat ccaaagtaag aactggcagg
    32281 ttcttggttg tctaacaacg cttcgctatg aaatgaaaac agctattggt ctcatggcac
    32341 tggatcagag ccgatacttt gatataacag attacgaatt ggagcttgaa gtggaaaatc
    32401 atgagcaagg caaacaggat ttccgacaat ttttagagaa aaatcagatt tcctaccaaa
    32461 aagctccttc aaaattggtt cgatttgtca aaagtatgaa aaatagctga aataatctcc
    32521 attttttggt aaaatagaaa agataaaaat acacaaatcc cagttcatat atgtaaggta
    32581 aatataacta ggatttataa agtttacaga ggacggtcta taatgtcaga tagaaaaaac
    32641 atgaaacttt tcgcactcaa ctctaaccaa gagattgcac aaaaaattgc ccaagctgtt
    32701 ggtgtcccac ttggaaaact atcatcacgt caattttcag acggagaaat ccaagtaaat
    32761 atcgaagaaa gtgtccgtgg ttatgatgtt tacatcatcc aatcaacaag tttccctgtc
    32821 aacaaccacc taatggaatt gttaatcatg gtcgatgctt gtgtgcgtgc aagtgcccac
    32881 agtatcaacg ttgtccttcc atattttggc tatgcacgtc aagaccgcat tgcttgtcct
    32941 cgtgagccac ttacagcaaa actagttgcc aatatgctgg ttaaggctgg agttgatcgt
    33001 atcctgactc ttgatttgca tgccgttcag gttcaaggtt tctttgatat tccagtggat
    33061 aatcttttca ctgttcccct attcgcaaaa cattactgcg ataaaggatt gcttggttca
    33121 gatgttgttg tcgttagccc taaaaattca ggtgtcaaac gtgcgcgtag cctggctgaa
    33181 tatcttgatg ctcctatcgc cattatcgac taccctcaag acgatgcaac tcgtaacgaa
    33241 ggttatatta ttggtgatgt tgaaggtaag aaagctatct tgattgatga tattttaaat
    33301 acaggacgta ccttctctga agcttctaaa atcgttgaac gtgaaggagc tacagaaatt
    33361 tatgctgttt ctagccacgg tctcttcgtc gagggagctg ctgagcttct tgacaatact
    33421 aatattaaag aaattcttgt gactgattca gtagcaacaa aagaaaaaac tcctaaaaat
    33481 gtatgctaca tcactgctag tgagttaatt ggtgatgcta tcgtccgtat tcacgaaaga
    33541 aaaccagtca gcccactctt tgcctacaat aaaaagaaat aaggtgattc tttgatttat
    33601 ttggacaatg ctgcaacgac tcctatgtca gcagttgcta tttcagctat gaccaaggtt
    33661 atgcaagaaa cccacggaaa tccttctagt attcatggtc atggtcgtca agctggcaaa
    33721 ctcttgcgag aagcccgtca ggaactagcc cagttactaa ggacaaaacc tcaacatatc
    33781 tttttcactt ctggtgggac tgaaggcaat aatactacca tcattggcta ctgtcttcgt
    33841 caccaagaac aaggaaaaca tatcatcaca actgccatcg agcaccatgc tgtccttgaa
    33901 acaattgatt acttggttca acactttggg tttgaagcaa ccattatcca gccagaaaat
    33961 caagaaatca cagcccagca aattcaaaag gctttacgtg acgatacgat tttggtttct
    34021 accatgtttg ccaataatga gacaggaaac ctactgccca tcgctgaaat tggccaaata
    34081 ctcaagcaac accctgctgc ctatcatgtt gatgcagttc aggctattgg taaaatccca
    34141 attcattcag aagaattggg cattgatttt ctcactgctt ctgcccacaa attccatggt
    34201 cctaagggaa tcggttttct ctacgcatct agcatggact ttgattccta tctacatggc
    34261 ggagaccagg aacagaaaaa acgtgcagga actgaaaatc tgcctgccat cgtaggcatg
    34321 gttgcagccc taaaagaaga cctagaaaaa caagaagaac attttcaaca tgtacaaaat
    34381 ctagaaactg cctttctggc agagctggag ggcattcagt attacctgaa tagaggaaaa
    34441 catcatctcc cttatgttct caatattgga tttcctggtc agaaaaatga cctcttactc
    34501 cttcggctag atttagctgg aatttcaatc tctactggct cagcctgtac tgcaggcgtt
    34561 gtccaatcca gccatgttct tgaagccatg tatggcgcaa attcagaacg cttgaaggaa
    34621 tcccttcgca tcagtttgtc gccacaaaat accgttgaag acctacaaac cctcgcaaaa
    34681 accttaaaag aaattatcgg aggttagcca tatggcattt gaaaaaatca ttcagttaaa
    34741 aaattgtcgt tacgattaca ctcttagccc ttctgttaaa aaattcaccc tcaaagataa
    34801 cacctttttt gaaactaagg ttggtaacta tgaactgact cgccttttgg aaaaagtgcc
    34861 aaacagcggt gaaggcttcc aactcaaaat catcattaac aaggaactta caggggctaa
    34921 aatcaatatc actgacaagt ttggccttcg tctagttgat attttcaaat cagaagacca
    34981 ccatattcat caggaaaaat tctacttcct catggatagc ttggtagaac gtggtgtctt
    35041 tacaaaatcg gaaagataga gccaaaatgt ttgaactgac ctataaggac tgctatcatg
    35101 tagagcgtac tctcaagtat gaggatcatg aggctctcat gttgactttg tcaggatgtg
    35161 taaccttacc agatacactt tatgtgactt ctctgacttt taggggccaa aaagtttatc
    35221 agggtctggt cggagacctc taccgttttc tatctcatgc agatttttta catcaaaact
    35281 aagatttttc ttagtttttt cttatttttg ttgatttttt cacaatgttt ctataaaata
    35341 gaagaataga aaggttgtga ttttgtgaaa gataaacagt ttgctattcc aaaagctaca
    35401 gcaaaaagac tctctctcta ctatcgaatt tttaagagat ttcatgcaga aaagattgaa
    35461 cgtgccaact ctaagcaaat tgcagaggct atcggtattg attcagcgac cgtacgtcgt
    35521 gatttttcct attttggtga acttggtcgt cgtggttttg gctatgatgt caaaaaacta
    35581 atgacatttt ttgccgattt gctcaatgac aactctatta ccaatgtcat gctggttggt
    35641 attggaaata tgggccatgc ccttctccac taccgcttcc acgaacgtaa caagatgaag
    35701 attatcatgg cctttgacct agacgaccat cctgaagtcg gtacccaaac tcctgacggg
    35761 attcccattt acgggatttc tcagatcaag gataaaatca aggatgctga tgtgaagact
    35821 gctatcctaa ctgttcccag cgtcaagtca caagaggttg ctaatctctt ggttgatgct
    35881 ggtgtgaaag gaattctcag tttttcacca gtccatctgc atttaccaaa agacgtggtc
    35941 gttcagtatg tcgatttgac aagtgaactc caaaccctcc tctacttcat gcgaaaagag
    36001 gattagaaag cgaaaatcat tttatgaaaa aaccagttat tgggattaca ggaaacgaaa
    36061 aagctcatcc agatgatgac atcatgatga gctacgcagc aaaaggcttt gttgaaggcg
    36121 ttaaagatgc tggagggatt cccatcatcc taccgattgg tgatcaagaa atggcctgcc
    36181 actatatcag tttgattgac aagctcatct tgacaggtgg gcaaaatgtc gatccaaaat
    36241 tctatggcga acccaaaacc attgatagcg atgactatca ccttcaaaga gatatcttcg
    36301 aactggccct catcaaggaa gctattaaac agaaaaagcc cattttctct gtctgccgtg
    36361 gtacccaact ctttaacgtt gccatgggtg gaactttgta ccaagatatc gaagaccatt
    36421 ggcaggattc ttctgttgag tacacaactc aacgcttggt aacagaacca gataccgttc
    36481 tccaagaaat ctatggagaa atctctcata tcaactcctt ccaccaccag agcatcaagg
    36541 acttagcacc aaatttaaaa gttgtggctc atgatcctaa agatggtatc attgaggctg
    36601 tcatgagtac ggatgatgtc gcctttctcg gtgtccaatg gcatccagaa cttctttttg
    36661 aaaatcgtcc caaagataaa aaactctttg actatgtcgt taatgaactt tagattaaat
    36721 ctgtcttttc acgataacta aagtaattag aatgagagac aatcaaatgg tccaagagaa
    36781 caatccccat cagttcgcag gcttctttaa caagtttagt gacatgatca tcattttggc
    36841 taggcgctac cgctcctgaa ggatgattgt ggaccaagat aagagaagtc gccatatgct
    36901 tgattgcata gtgaagaatc tctcgcggtt cagcgatact acgagttaca gaaccgataa
    36961 aaatggtctg ctgatggatg atttgatttt gagtattgag atagagtgcc accaggtgct
    37021 cttgtttttt atcccctaat tcctgctgca tcttcttggc caacttttga ctgctgagaa
    37081 tactttccat ttcaagagtc tcgtgtttgt gaatacgatg ccccagttca atcatagctt
    37141 gtaattctat ggccttaaca cgcccaatac cagacaaact ctgcaattcc tgcagggtca
    37201 tttttttcaa atccgttagg cttgaaagat tgttcaagac tttttgggca atttcaaaaa
    37261 cgctagcttg acgtgttcct gtcctgagta aaatagctag caactcttgg ttactaagcg
    37321 cttcaactcc ttccttggcc agcctttctc ttggtaatag tgaatcttct tggaatgaaa
    37381 tactgtacat aaaaatcctc ctcactttat tattcgtgag aaggatggaa aattagattt
    37441 ttttctcaat tggagccaca ctggctaaaa gttttttcaa acctacttct gggaaattga
    37501 ttttcaattc ctgcctagca ccgctacctg aaacttccag aacagttccc tctccccatt
    37561 tcttgtggag agcaatgtca ccaatggacc aatttgcctc gctagatgct gattttgcgc
    37621 cagctgtaaa ttgaccaaat ggaagaccgc ttgactggat tgattttggg gcagcaccgc
    37681 gtttacggtc ttgaagagcc tgagccaaac tcataccttg accaaaggaa atactaccac
    37741 tgctatatga tgccttaaag cttgtatttg caggacgagc cagaccttga tactcaagca
    37801 agtctgaact gatttcgtta ataaaacgag tcggacggtt ataattggta cgaccaaaaa
    37861 gcaagcgtga gttggcattg gtcagataga gaattttctc tgcacgcgtg atacctacat
    37921 aggctagacg gcgctcttct tctaattcat ctggatcttc agtcgcacga ctaagtggaa
    37981 agacattttc ttccatccca atcaaaaaga caactggaaa ttcgagacct ttggcagcat
    38041 gcagggtcat caaggtcact tctgatgtct cctgactacc tgaatctgtg tcggcaatca
    38101 aagccaagtc atttaagaaa cgactcagtt tgtccagacc agtttcctct tctgtcacat
    38161 cagtggtgtc atcaaagttc ttcgtaacag aaagaaactc ttcgatattt tcaacccgtg
    38221 ccttgctttc tagagtcgct tgggcgttaa gaatatcgac ataacctgtt ttttctagga
    38281 cggactcaac caactctgta atgcttaagt ggtctagctg ctcccgcaaa tcaagcatca
    38341 tattggcaaa atcccagata gattgggctg ccttaccctt gataccagac aacataatat
    38401 tagcagaagc atccagcata gacatatttt gcaaatttgc aaaatcacgg attttctcaa
    38461 ctgtacctag accaattcca cgtttaggct cgttgataat acgctcaaaa ctaatattgt
    38521 cactcaaatt agcaataagg ttgagataag caataatatc gcgaatttcc ttacggctgt
    38581 agaatttggt tccaccaacc atggtataag gaatgttaga cttgagcagg gcttcctcaa
    38641 ttgtacggga ctgggcatta gtccgataga gaactgcaaa atccttatga aggaagtttt
    38701 gactgcgact aagttcatcg atggttctgg ctacaaatac agcctcatcc agctcatcat
    38761 cggcacgata gtaaacgatt tgctccccat cagcgttttg agtccagaga tttttaggac
    38821 ggcgattttt attattttta ataacctcgt tggccgcttg gagaatggtt ttggttgagc
    38881 ggtaattttc ctccaacaaa acaaccttgg ctttggggta atccttttca aagtccaaga
    38941 tattctgcat atcagcacca cgccaaccgt agatagactg gtccgcatcc ccaaccacac
    39001 agatattttt aaaacgggaa gccaagagtt tgaccaattg gtactgagcg tggttggtat
    39061 cttggtactc atcaacgtgg atgtattgga atttttgctg gtagtaggtc aaaacatcag
    39121 gattttgatc aaagagacgc aaggtcagca taatcaaatc atcaaagtca acggattcag
    39181 actgacgaag ttctttttga taggttgtat aacactgggc cacaatttgc gtatacatat
    39241 cgccagcttg ggcagcataa gcaacatcat caatcaaatc attcttagca ttggaaatgg
    39301 tccccaaaat agttcgttca ttccattttt taggatccaa gttcaactgt ttgagaatac
    39361 gtttcatgag cgttcgctgt tcaccaggat ccacaattgt aaaattacga ttgtagccaa
    39421 tatggtccgc atcgcgacgc aaaatacgca cacacatgga gtggaaggtc gcaatcagac
    39481 agtcctgagt agctggattg aggctataag cacgctcttt catctcacgc gcagccttgt
    39541 tggtaaaggt aatggccaag atattccaag gattgaccag cttttcatca atcaaataag
    39601 cgatacggtg ggtcaaaaca cgagtctttc cagaaccagc ccctgccatg attagcaagg
    39661 gaccttctgt cgtttgcacc gcctcagcct gacggtcatt cattccattt aataatgcgt
    39721 tcatcttctc ttccttactc ttgaagtcaa tatttctatt atatcagaaa tcagggaaaa
    39781 tagaaacttt acggggagta gcaaaatata gtggattgaa actagaatac tacactgtgg
    39841 gtttctaaaa cactcgactt ttcacccaat tttttcataa actagctcaa tacctcaata
    39901 gacttcctgc aaaactagaa tcctagttca tgattgataa taccagcaat aaaattcatt
    39961 cgtaatccga agcgtttacg atgatttcga taagttgttg aaaacatttt aaacgttttt
    40021 actttggcaa agatgttctc aaccttgctt ctctccttag atagcgcatg gttataagcc
    40081 ttatcttcaa ctgttagcgg cttgagtttg caggatttac gtggagtttg tgcttgagga
    40141 tatattttca tgagcccttg ataaccactg tcagccaaga ttttaccagc ttgtccgata
    40201 tttctgcgac tcattttgaa caacttcata tcatgacaat agttcacagt gatatccaaa
    40261 gaaacaattc ttccttgact tgtgacaatc gcttgagcct tcatagcgtg aaatttcttt
    40321 ttaccagaat aattcgctaa ttcttttttt agggcgattg gtttttactt ccgtcgcatc
    40381 aatcattacc gtgtcctcag aactgagagg agttcttgaa atcgtaaaac cactttgaac
    40441 aagagttact tcaacccatt ggctccgacg gattaagttg ctttcgtgaa taccaagatc
    40501 agccgcaatt tgttcataag ttcgatattc tcgcacatat tgaagagtgg ccataagaag
    40561 gtcttctagg cttaatttag gttttcttcc accttttgca tgtttaagtt gataagctgt
    40621 ttttaataca gctaacatct cttcaaaagt ggtacgctga acatcaacaa gacgcttaaa
    40681 tcgtgcatca gttagttgtt tacttgcttc atcattcata gaactactat accatgtttt
    40741 gtttcgcagg aagtctaatc taaaaaaggt aaatttaaga ttggcaatac gaacgttaaa
    40801 cagaattcca tttctctttt tagaatcaaa acgacaatat tgtggaatct ctacaggaac
    40861 aatagactgc gacactaaaa tatcctccct tagttttact ttactaatag tatataggag
    40921 gagagccctg aatcctagac cctttgttat gatgcgctta caaacagagt tggaaaaaat
    40981 cttaaaagca gaaaaacgca tgatatcagc ccctaacact tgatacaatg cgttttatta
    41041 tgaaaagatc tactggattt tttatcaaaa taaacttcca gtagtctttt cactttttat
    41101 taataagttc cttcttcacc ttggctagtc aagataacag gtccatcttt cgtaatgaca
    41161 aattggtgtt catactgaca tgacaatcca ccgtcaatgg tcttatgcgc ccaaccagtt
    41221 ttcatatctg tatcaatttc ccaatcgcct gtattgatca ttggttcaat ggttaagacc
    41281 attccttcac gaagacggag tccacgacct gcaataccat agttaggaac cattggttct
    41341 tcgtgcatag ttgggccaac accatgacca accaaatcac gcactacacc gtaaccacga
    41401 ctttcagcgt attcttgaat agccgcaccg atatcaccga tacgatttcc aacaacagct
    41461 tgctcaatac ccttgtacat agcttctttg gttacatcca tcaagttttt gacttcttcg
    41521 gacggtgtac caacagcata agcccaacat gagtctgcta aaccaccaga atagctctga
    41581 gtatattttt tcatttgttc aacattgttg aagtttaatt ttgagacatt caggtcagat
    41641 ttagcaatgg gacctcccaa aaccatatca actttgagca aatcaccatc tttcaagata
    41701 taatgacgag ggaaagcgtg agccacttca tcgttaagag agcaacaggt agcataagga
    41761 tagtccatca tggcaccgtc aaccccaatc tgaagtggaa ggaaattttc ttctttacaa
    41821 cgacggcgga catattcttc aacttcccac atatctacgc ctggcttaat caaatcacgt
    41881 aagcctatat gaatacttgc tagaaaatca ccagccttgt ccatagcttc gatttcacga
    41941 gctgatttta atgttatcat cttttctcct agtttctaat taattttaac agtcacattt
    42001 gcttttgaaa caatctggtg accatgataa atatcgtaat caattatagc tgaccgtctc
    42061 gtatgatgaa taatccgtgc ctgaatgcgc aatatatcat ctatctgaac agcctgcaaa
    42121 aagtagatca gcatctgctc gataatgaga ttgcgaccac tattaacaac taaatcttgg
    42181 gtcatgtggg tcagaatttc tgccaataca ccattagcca aaactccatt tttttctagc
    42241 ataaagggtt ccactgtaat gactacttca tcatggtgat aagagagctt ttgtccaatc
    42301 tgctcagaaa aagttggtag agccgaaact tgagaacggc tcatcttctc catgacatct
    42361 cgtcgcgtca caacgccaag caaagtttga ttgcttcgaa caactggtac catttcaaag
    42421 tcttctgcga tcatccgttg actcacattg gcaatatttg tcgataaccc aaccaaaaat
    42481 agactacgag acataacctt atcaattgtc gtgcttggtg atttatcacc agcgtctctc
    42541 atggttacaa cgccaacaac gacctgatgt tgattgataa cagggaaacg gctgctacga
    42601 ttcttacgaa ccaagtccaa ataatcttta actgtatctg tctctctcag aaaaccatac
    42661 tcatgactag ggcgataaag tttctcaact gtcagaatat cagtcttgat ttggacattt
    42721 gacaaggctt tattgatcat ggtcgcgacg gtaaaggtat catgcttact tcttagaaca
    42781 ggaatccctt tttgattggc cagtttaagc acatcatcat gaacctgaaa tccccctgta
    42841 accagaactg cattttcatt ttccaaggct agcaactgaa tacgggttcg gtctccgaca
    42901 atcaagagcc ccccatcatg aaggtaagac aagatatttt gttcagtcat ggcaccaatt
    42961 gaaaacttac taaactctct ctctaaacct tcttgcccag ccagaacctc agaagaagtc
    43021 acttctgcaa tttcagcaaa cgttaatctc tctatagcaa ctttctggga tttaacacga
    43081 attgttccac ttctaggacg ggtctccaca attccacggt tttcagcttc tttaatagcc
    43141 cgataggctg ttccatcact aactcctaga tgattcgaaa tgctacgaac actgaccctt
    43201 ttacctactg gtaattcctc caaatagctt agaatttcct gatgcttact cattgaactc
    43261 tccttttaca taccgaaatt caagataatc ccgcattttt cttgtgtaac gatcacttcc
    43321 cttaaactga cggaacaaac taaatccaga cttaagagca actctttggc tagcttcatt
    43381 ttcaaggtgg gtaatgatag ataattgttt taagccaaat tcctcaaaag aaagctgaca
    43441 aatttttcta acaacctctg tcataaatcc ttgcgaccaa gcatcttttc tcaaaaaata
    43501 gccaagctca gcttcttttt tgatttcatc taacttctca aatttaatag aaccaatcat
    43561 ttgttgattt ttctggtcac aaattgccca cactcccaaa ggggacttca taaagtaatt
    43621 ggccagtgca tattgacttt cttccagact tgcctgcgtt gggaaaataa attgaagatt
    43681 ttctggattt gaagctatct cgcggaagtc ctgactatca ctaaaaaaga aaggacgcaa
    43741 atacaagcga tccgtttcaa aaaaagaaaa cattgctaat ttggtccaaa tattcataaa
    43801 cacctctacg gtttaatcct caacaagtct aatatccgct cctaaattac gtaatttttc
    43861 gataatatca gaataaccac gtaagataaa ctcgatattg gtaatttcag ttttaccttc
    43921 agccataagc ccagcaatga ctagtgcagc cccagctctt aagtcggtcg ctttaacact
    43981 ggccccacgt aaatcacgtc cacccgtgta caaaatatga ccatttgttg tcgaaatatc
    44041 cgcatccatc tttgctagtt caaaaacatg atttacacgt ttttcgtaaa tcgtatcgac
    44101 aattgtacca cgaccattcg ctcttagtaa aagaggggta agcggttgtt gcaaatcagt
    44161 tgcaaagcct gggtaaggag ctgtcttaat attgattgct ttcaaattag actgttcctc
    44221 gacaaaaatg ctgtcttcag atacagtcat tctcactccc atttcttcca acttagcaat
    44281 aaacccttcc aggtgttcgt aaagaacatt atttatacga attcctttac caactgcagc
    44341 agctaaagat atatatgttc cagcttcaat gcggtctgga atcacctgat gacgtgtccc
    44401 atgtaatctt tcaacaccat caataatgat gatattagtt cctgccccac ggatatgggc
    44461 acccatatta ttcaagagag tagctacatc aataatctca ggttcacggg ctgcattttc
    44521 aataatagta cgaccatttg ctttaaccgc agcaatcatc gtattaatcg ttgctcccac
    44581 actaaccgta tccatgtaaa tacttgcacc atgaagtcct gtatctttag cagataactt
    44641 catgttatct ccctcgtagc tagcagtggc acccatagct tcaaacgcct taaggtgtaa
    44701 gtcaatcgga cgaggaccaa gatcacatcc tcccggtaga ccaactgtcg cttcaccaaa
    44761 acggcctaag aggctcccat aaaaataata agatgcacga agactgttaa ttttaccata
    44821 aggcattgga atattttgaa cacctcttgg atcaatctcc aagacatcgt cataacgctt
    44881 aacagtagct cccatcaatt ccatgatttc gacaagactg gctacatccg aaatatctgg
    44941 aacacaatcc aaagtcacca catcatcagc caatataata gctggaatta aggccacaac
    45001 actattttta gcaccactaa tagtaatttc accttgcagt ggtaatccac cattgataac
    45061 aatttttctc attctaagtt ttcaatactc tttcaagatt tctaataagg tcttcaaaat
    45121 aaagtatatc ataaatttat ccttttttcc atccttttca attttattgg ataaatcaga
    45181 gttgacgagt tagtaactga attactaata ctcaataaaa atcaaagatc acactaggaa
    45241 actagccgca ggttgctcaa aacactgttt tgaggttgta gatggaactg acgtagtcag
    45301 tatcctacac gtacggcaag gcgatattga cgtgatttga agagattttc gaagagtata
    45361 atctttctcg aatcgctatt gccccttttt tatcccctga aaccgagtag gagctaagac
    45421 tagatctctt atctaaatat cggaatacgg ctgttcaact aaattttagc aagaacttct
    45481 cccccttctc tcagttcaca gaagcgtttt atacataccg tcctatattt catgaacaag
    45541 acgttttcag gctagcatcc tatcagacaa tctattttaa agataaccgt gactcaggat
    45601 cacttctatc tgtcgttttc tctttatagt gatagacctc acgggataag tcgtacatct
    45661 ttcctcgtgt atacaagata tgaggacgtt aaaaggttac agtgaaaata gaaaatctta
    45721 gtacgaattc aattactctc gtgatttacg catataggtt acgactacct ttttggactt
    45781 tagagtttga ggccctctta tccgctatat acgccttatt atcacgttcc tgttcgtaga
    45841 gccacgattt cgctatccct tcttctgaca attcttattt ccagcgtcag acttaaaacg
    45901 gtctatcccc agaccatttt aatccgctac ctcacgatag tcaggcttgg gaatcgctat
    45961 ttgattcgcc gagaactttg acatcagata tactataaca aattgaaata agaatcaaac
    46021 aatttaattt tgaaattcaa atcaatttat aacaatattt tagaagatat gctgtgctat
    46081 tccagattca gtttgctata catgcctgtc gtaccacaaa atcccgtgcc tcatcaggca
    46141 cgggattttg gctctataat atttgtagtg ggtaaatcca ctatagatat tatggagcct
    46201 attttactgt agaaaaaaaa tcccataaga tctataatga aaagcgacaa aacaactcat
    46261 tagaaagatt catatgggac aattacattt tatcacaaaa ttactagaca ttaaagaccc
    46321 taatatccag attttagaca tcatcaataa agatacacac aaggaaatca tcgccaaact
    46381 ggactacgac gcaccatctt gccctgagtg cggaaaccaa ttgaagaaat atgactttca
    46441 aaaaccgtct aagattcctt accttgagac aactggtatg cctactagaa ttctccttag
    46501 aaagcgtcga ttcaagtgct atcactgttc aaaaatgatg gtcgctgaaa cttctatcgt
    46561 caagaaaaat caccaaattc ctcgtattat caaccaaaaa attgcgcaaa agttgattga
    46621 gaagatttct atgaccgata ttgctcatca gctggccatt tcaacttcaa ctgttattcg
    46681 taagctcaat gattttcact ttaaacatga tttttcttgt cttcctgaga ttatgtcctg
    46741 ggacgttgaa acagtccggg gagtgactgt ttcaatcggg agatggagat gagctttatt
    46801 gcgcaagatt ttaacaatct caatatcatc actgttcttg aaggcagaac acaagctgtc
    46861 atccgagatc actttcttaa atatgataga gccgtccgat gtcgcgtcaa aatcattac
//
LOCUS       DAVDWT010000013        46476 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVDWT010000013 DAVDWT010000000
VERSION     DAVDWT010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.013"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            1..971
                     /locus_tag="VUW50_000873"
                     /pseudo
     CDS             1..971
                     /locus_tag="VUW50_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1087..1890)
                     /locus_tag="VUW50_000874"
     CDS             complement(1087..1890)
                     /locus_tag="VUW50_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HET7982796.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(2270..3874)
                     /locus_tag="VUW50_000875"
     CDS             complement(2270..3874)
                     /locus_tag="VUW50_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7982797.1"
                     /translation="MSEYWFSTNVDQIDEVDGKQCLIYSYYNVKASRNVEVLKGRSGT
                     KKGLDYWEPYAPQKQYEMERLPKNKYIGSSSTDRWDGIEKNVVFCDCKEYVSAFDLFF
                     YHYNFKKISTQRSKQDFIRLRSKPVADILKNNTSSYTRYKKEMVIDNVKVDDKVCEII
                     SEIMDESYTDIQILTHKLYSKGDDIKASKTIWMKKSGKEYSEAFAGTGEARIILLVND
                     IVNAQSNSLILIDEPEISLHPSAIYKFKEFLLQECLNKKHQIIITTHSTQLIKDFLRE
                     AVKLLVKNGEKVDVIENIDYQDAFFELGDVYHSRKMIYVEDRLAKYILEFVITHSGSE
                     NLKQNLVVRYIPGGANQIICNNILNSSYLDSDNHYFWLDGDQNTNVSESNNLMNYLEN
                     GVVISDKIPESDNKNLDDIIKLITGCPIKFNVSGNKGQKNNIELIAKQRSFIDYWAKY
                     VSYLPFPTPEFFLANLCNSVDREGYDFSKDGNGKEYFRKKTQVALGIENITSEDIFQE
                     QRRAVSKIQPESSMFQCIKEKLEALF"
     gene            complement(3925..4089)
                     /locus_tag="VUW50_000876"
     CDS             complement(3925..4089)
                     /locus_tag="VUW50_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HET7982798.1"
                     /translation="MGKIVAIDLFNGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDME"
     gene            complement(4170..6821)
                     /locus_tag="VUW50_000877"
     CDS             complement(4170..6821)
                     /locus_tag="VUW50_000877"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET7982799.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVIAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHSIMPFVTEEIFGQYAEGSIVTAAYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDVTVVRIDEMKKLVK"
     gene            complement(6814..7392)
                     /locus_tag="VUW50_000878"
     CDS             complement(6814..7392)
                     /locus_tag="VUW50_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7982800.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQSLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKYKPLKRHIEVSEREIIETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(7392..7952)
                     /locus_tag="VUW50_000879"
     CDS             complement(7392..7952)
                     /locus_tag="VUW50_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7982801.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHIFPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLDFTLEARI
                     RDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            complement(7939..8516)
                     /locus_tag="VUW50_000880"
                     /pseudo
     CDS             complement(7939..8516)
                     /locus_tag="VUW50_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809142.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            complement(8799..9194)
                     /locus_tag="VUW50_000881"
     CDS             complement(8799..9194)
                     /locus_tag="VUW50_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET7982802.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQPAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(9290..9544)
                     /locus_tag="VUW50_000882"
     CDS             complement(9290..9544)
                     /locus_tag="VUW50_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HET7982803.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            complement(9554..10888)
                     /locus_tag="VUW50_000883"
     CDS             complement(9554..10888)
                     /locus_tag="VUW50_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HET7982804.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYEYMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKDDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(10888..11121)
                     /locus_tag="VUW50_000884"
     CDS             complement(10888..11121)
                     /locus_tag="VUW50_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HET7982805.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            11152..11229
                     /locus_tag="VUW50_000885"
                     /pseudo
     CDS             11152..11229
                     /locus_tag="VUW50_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(11570..11752)
                     /locus_tag="VUW50_000886"
     CDS             complement(11570..11752)
                     /locus_tag="VUW50_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982806.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(11754..12533)
                     /locus_tag="VUW50_000887"
     CDS             complement(11754..12533)
                     /locus_tag="VUW50_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982807.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGIAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
     gene            complement(12813..13163)
                     /gene="rbfA"
                     /locus_tag="VUW50_000888"
     CDS             complement(12813..13163)
                     /gene="rbfA"
                     /locus_tag="VUW50_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET7982808.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            complement(13414..16290)
                     /gene="infB"
                     /locus_tag="VUW50_000889"
     CDS             complement(13414..16290)
                     /gene="infB"
                     /locus_tag="VUW50_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET7982809.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAAPKASAEKKAE
                     KSEPVKPAVAKEEAKPAEPVTPKTEKVAAKPQSRNFKAEREARAKEQAERRKQNKGNN
                     RDQQQNGNRQKNDGRNGGKQGQSNRDNRRFNDQAKKQQGQQKRRNERRQQEDKRSNQA
                     APRIDFKARAAALKAEQNAEYARSSEERFKQYQAAKEALAQANKRKEPEEIFEEAAKL
                     AEQAQQVQAVVEVVPEKKEPAVDTRRKKQARPDKNRDDYDHEEDGPRKQQKNRSSQNQ
                     VRNQKNSNWNNNKKNKKGNNKNNRNQTPKPVTERKFHELPTEFEYTDGMTVAEIAKRI
                     KREPAEIVKKLFMMGVMATQNQSLDGETIELLMVDYGIEAKQKVEVDNADIERFFVED
                     GYLNEDELVERPPVVTIMGHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIVENG
                     KKITFLDTPGHAAFTSMRARGASVTDITILVVAADDGVMPQTIEAINHSKAANVPIIV
                     AINKIDKPGANPERVIGELAEHGVMSTAWGGDSEFVEISAKFNQNIEELLETVLLVAE
                     IQELKADPTVRAIGTVIEARLDKGKGAVATLLVQQGTLNVQDPIVVGNTFGRVRAMTN
                     DLGRRVKVAGPSTPVSITGLNEAPMAGDHFAVYEDEKSARAAGEERAKRALMKQRQAT
                     QRVSLENLFDTLKAGELKSVNVIIKADVQGSVEALSASLQKIDVEGVKVTIVHSAVGA
                     INESDVTLAEASNAFIVGFNVRPTPQARQQAEADDVEIRLHSIIYKVIEEMEEAMKGM
                     LDPEFEEKVIGEAVIRETFKVSKVGTIGGFMVINGKVARDSKVRVIRDGVVIYDGELA
                     SLKHYKDDVKEVTNGREGGLMIDGYNDIKMDDVIEAYVMEEIKR"
     gene            complement(16307..16606)
                     /locus_tag="VUW50_000890"
     CDS             complement(16307..16606)
                     /locus_tag="VUW50_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HET7982810.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            complement(16599..16892)
                     /locus_tag="VUW50_000891"
     CDS             complement(16599..16892)
                     /locus_tag="VUW50_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HET7982811.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            complement(16914..18050)
                     /gene="nusA"
                     /locus_tag="VUW50_000892"
     CDS             complement(16914..18050)
                     /gene="nusA"
                     /locus_tag="VUW50_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HET7982812.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            complement(18094..18573)
                     /gene="rimP"
                     /locus_tag="VUW50_000893"
     CDS             complement(18094..18573)
                     /gene="rimP"
                     /locus_tag="VUW50_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET7982813.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            complement(18699..19334)
                     /gene="trmB"
                     /locus_tag="VUW50_000894"
     CDS             complement(18699..19334)
                     /gene="trmB"
                     /locus_tag="VUW50_000894"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET7982814.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            complement(19331..20125)
                     /locus_tag="VUW50_000895"
     CDS             complement(19331..20125)
                     /locus_tag="VUW50_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET7982815.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            complement(20287..20898)
                     /locus_tag="VUW50_000896"
     CDS             complement(20287..20898)
                     /locus_tag="VUW50_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7982816.1"
                     /translation="MEFFDKFHALCFGFLVIIIVITVPYTINHGDFFQNESALIIVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGAGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            complement(20928..21176)
                     /locus_tag="VUW50_000897"
     CDS             complement(20928..21176)
                     /locus_tag="VUW50_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982817.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFLGAGYVVGFWLLKWFVLERLELKDDA"
     gene            complement(21218..21907)
                     /locus_tag="VUW50_000898"
     CDS             complement(21218..21907)
                     /locus_tag="VUW50_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7982818.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNLF
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFLSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            complement(21959..22342)
                     /locus_tag="VUW50_000899"
     CDS             complement(21959..22342)
                     /locus_tag="VUW50_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET7982819.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRKRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            complement(22971..23342)
                     /locus_tag="VUW50_000900"
     CDS             complement(22971..23342)
                     /locus_tag="VUW50_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982820.1"
                     /translation="MIKKNHILCEIVPGAVAFACFYLSISISSVFQFLLVVINLASIL
                     VTVYLTREMRIKEFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQLIAYI
                     GAILCLIIMLLLIWGLKYYKK"
     gene            complement(23811..23930)
                     /locus_tag="VUW50_000901"
     CDS             complement(23811..23930)
                     /locus_tag="VUW50_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HET7982821.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            complement(24034..24183)
                     /gene="blpO"
                     /locus_tag="VUW50_000902"
     CDS             complement(24034..24183)
                     /gene="blpO"
                     /locus_tag="VUW50_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HET7982822.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(24427..24630)
                     /gene="blpN"
                     /locus_tag="VUW50_000903"
     CDS             complement(24427..24630)
                     /gene="blpN"
                     /locus_tag="VUW50_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HET7982823.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGKILGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(24646..24900)
                     /gene="blpM"
                     /locus_tag="VUW50_000904"
     CDS             complement(24646..24900)
                     /gene="blpM"
                     /locus_tag="VUW50_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HET7982824.1"
                     /translation="MNTKMMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            25180..27305
                     /gene="blpA"
                     /locus_tag="VUW50_000905"
                     /pseudo
     CDS             25180..27305
                     /gene="blpA"
                     /locus_tag="VUW50_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter BlpA"
     gene            27316..28677
                     /locus_tag="VUW50_000906"
     CDS             27316..28677
                     /locus_tag="VUW50_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HET7982825.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ETQLQRLEKQKEGLGILKQSLEKATDLFSSEDEFGYHNTFMNFTKQSHDIELGISKTN
                     TEVSNQANLTNSSSSAIEQEITKVQQQIGEYQELRDAIVNKRARLPTGNPHQSILNRY
                     LIASQEQTQGTAEEPFLSQINQSIAGLESSIASLKLQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPIITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTAKKTYFDYFKDKILT
                     HSD"
     gene            28734..28889
                     /gene="blpC"
                     /locus_tag="VUW50_000907"
     CDS             28734..28889
                     /gene="blpC"
                     /locus_tag="VUW50_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HET7982826.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGWWEELLHETILSKFKITKA
                     LELPIQL"
     gene            complement(28931..30271)
                     /locus_tag="VUW50_000908"
     CDS             complement(28931..30271)
                     /locus_tag="VUW50_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7982827.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLLKFLL
                     GIIFTTFQFLAVSKYLSYFIEPLFGIGLSFLLLRGLPKKILIFYGLFPMILVELFYRG
                     VSYFVLPFLGQGIVDGDGNPIFLLIMIFVCFIVLVFLKWLDYDFTRLRREFLDTGFQK
                     SLTKINWAMGAYYLVMQSLSYLEYEQGIQSTTVRHLILVFYLLFFMGGIKKLDTYLKE
                     KLQEELNQEQTLRYRDMERYSRHIEELYKEIRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLRDSSQKLQDNKYDLGRLVNIRDRALKSLLAGKFIKAREKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASAEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTNLNTTCQNQVFRQVLTVIHAE"
     gene            complement(30285..31022)
                     /locus_tag="VUW50_000909"
     CDS             complement(30285..31022)
                     /locus_tag="VUW50_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7982828.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(31027..31365)
                     /locus_tag="VUW50_000910"
     CDS             complement(31027..31365)
                     /locus_tag="VUW50_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7982829.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            31641..31970
                     /locus_tag="VUW50_000911"
     CDS             31641..31970
                     /locus_tag="VUW50_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET7982830.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(32138..33187)
                     /locus_tag="VUW50_000912"
     CDS             complement(32138..33187)
                     /locus_tag="VUW50_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7982831.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFCQKASKFFTETG
                     LDWDYLISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(33184..33915)
                     /locus_tag="VUW50_000913"
     CDS             complement(33184..33915)
                     /locus_tag="VUW50_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982832.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLDQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            33983..34393
                     /locus_tag="VUW50_000914"
     CDS             33983..34393
                     /locus_tag="VUW50_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET7982833.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            34403..34690
                     /locus_tag="VUW50_000915"
     CDS             34403..34690
                     /locus_tag="VUW50_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982834.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            34852..35091
                     /locus_tag="VUW50_000916"
     CDS             34852..35091
                     /locus_tag="VUW50_000916"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982835.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSSKHCF
                     EVVDRTDEVSNHTHGKPTLTWFEEIFEEYHSPFHN"
     gene            complement(35413..36549)
                     /gene="dnaJ"
                     /locus_tag="VUW50_000917"
     CDS             complement(35413..36549)
                     /gene="dnaJ"
                     /locus_tag="VUW50_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET7982836.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGDGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGQGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(36951..37307)
                     /locus_tag="VUW50_000918"
     CDS             complement(36951..37307)
                     /locus_tag="VUW50_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HET7982837.1"
                     /translation="MSVLDDEYLKNTRKVYNDFCNQADNYEAAKGFIDNIPVECLTIY
                     REIILAEHDSCVKNDEAVRNFVTSVLLSAFVSAMVSAMISLEIQTYKFVIPFIIGMIW
                     TVFVFLMINWNYIGKY"
     gene            complement(37309..39132)
                     /gene="dnaK"
                     /locus_tag="VUW50_000919"
     CDS             complement(37309..39132)
                     /gene="dnaK"
                     /locus_tag="VUW50_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET7982838.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(39612..40136)
                     /gene="grpE"
                     /locus_tag="VUW50_000920"
     CDS             complement(39612..40136)
                     /gene="grpE"
                     /locus_tag="VUW50_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET7982839.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(40163..41197)
                     /gene="hrcA"
                     /locus_tag="VUW50_000921"
     CDS             complement(40163..41197)
                     /gene="hrcA"
                     /locus_tag="VUW50_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET7982840.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREEQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(41361..41870)
                     /locus_tag="VUW50_000922"
     CDS             complement(41361..41870)
                     /locus_tag="VUW50_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HET7982841.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            42478..44811
                     /locus_tag="VUW50_000923"
     CDS             42478..44811
                     /locus_tag="VUW50_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET7982842.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDTNGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQSNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            44824..46287
                     /locus_tag="VUW50_000924"
     CDS             44824..46287
                     /locus_tag="VUW50_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HET7982843.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            46287..>46476
                     /locus_tag="VUW50_000925"
     CDS             46287..>46476
                     /locus_tag="VUW50_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HET7982844.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDD"
BASE COUNT        14268 a         9841 c         8404 g        13963 t
ORIGIN      
        1 aaaaacgggt atactattgt tgagaaaaca agagggagag tacctgagag cggagaatgc
       61 catcctaaaa aagttaagag aactccgatt gaaggaggaa aaagagaaag aagaaagaca
      121 gaaattgttc aagaattaat gactgagttt tcgttagatc ttcttctaaa agccattaaa
      181 ctagctcgtt ggacctacta ctatcacttg aaacagctag ataaaacaga taaggaccaa
      241 gagcttaaag ctgaaattca atccatcttt atcgaacaca agggaaatta tggttatcgt
      301 cggatttatt tagaactaag aaatcgtggt tatctggtaa atcataaaag agttcaacac
      361 ttgatgaagt actcaattta caagctaaaa tgcgacagaa acgaaaatat tcttctcata
      421 aaggagacgt tggcaagaag gcagagaatc tcattcaagg ccaatttgaa ggctctaaaa
      481 caatggaaca gtgctacaca gatgtgacag aatttgccat tccagcaagt actcaaaagc
      541 tttacttatc accagtttta gatggcttta atagcgaaat tatcgcctat aatctttcaa
      601 cttcacccaa cttagaacaa gtacaaacaa tgttggaaca ggcattcaca gagaagcact
      661 atgagaatac gattctccat agtgaccaag gctggcaata ccgacacgat tcttatcatc
      721 ggttcctaga gagtaaggga gttcaagcat ccatgtcacg caagggaaac agccaagaca
      781 acggtatgat ggaatctttc tttgggattc tgaaatcgga aatgttttac ggttatgaga
      841 agtcgtttca gtcgcttaag caattggaac aagctattgt agactatatt gattactaca
      901 acaataaacg aattaaggta aaactaaaag gacttagtcc tgtgcaatac agaactaaat
      961 ccttcggata aattaattgt ctaactgttt ggggtcagta cacctaaaac tttgatgata
     1021 tacgttttct tgtgagaata tttacttcat ttttgcctaa aattcaatgt ttactcagta
     1081 tttggattat gaaaaatcaa ggtctaaatc tagatacatt ttttctgaag acaaatcatt
     1141 ttgaccaccg agcaagagat tttcaaaaaa agctgttaaa aactcaggac gtcgctgtaa
     1201 aatctttgca ttatctaata ccaaggcatc acgaaaatat ttggaatgtt gctgaaatgg
     1261 tgtattatca atatcaaaac caaactcacg aagatactga atcaaaaaga ccgttactgt
     1321 ccgagtgttt ccttcgcgaa atggatgaat ctgccagatt cctgaaataa aatgctggat
     1381 ttgtttaacc acatccgcct gagttagtgt cgcatatgca acttgttttt cctgattaaa
     1441 atcataatct aaggtcattt gaatcatgga gtaatcagag tacacaacac tttcaccatt
     1501 caaaacaggt tcattctttg tgatattggt ctgacgaaat tgccccaccg gaatagaggg
     1561 ttcaaatata tcttgaaaca actccttatg aatagcaagt aaggtcgcag gactaaagct
     1621 aaagcctctt cgagacaata gttctacaat acgtagagaa accaagtctg cctcttccgt
     1681 acttgcatca atagtatggt gataagccgt tgcatcctca taaacctgct cataagtcag
     1741 ttctccccgg gactgtttct cagccaaaga ttccatatac gctgatggca ctagattgtc
     1801 aactttctgc agaccaaaac ctatccgcca taaatcacgc ttcgcttcat aagacaagtt
     1861 tggattgtca atgttgtaag ttggttgcat agaaatatcc tttcaattgt ttttctgtgt
     1921 catcggtaaa tggtgaaatg ggtatggaat tggcgaaaat tataaaaatc cttcacaata
     1981 agttctatga aatatatata cttcatctta atagtgagca agctaaactt agcatttcat
     2041 gccctcatat gggatgttct ttgactaaat aatatgatta tcgagatata tctggataaa
     2101 tgaactaata agtctgacgc gcatacttat caaactcatt ggcatacacc tctatgaact
     2161 cgttggtctg ttcaaatccc aacacattac ctgagaagaa agttgcaatg ttgtttttgg
     2221 tgcgggtttg aatttaaaaa atttgttatg tagtacctaa tctaaggaat tagaacaatg
     2281 cctctaattt ttctttaata cactgaaaca ttgatgattc tggctgtatt tttgaaacag
     2341 ctcttctttg ctcctggaaa atatcttcag aagttatatt ctctattcct aacgctactt
     2401 gagttttttt tctaaaatat tcttttccgt tgccatcttt agaaaaatca taaccttccc
     2461 tatctacgct gttacacaaa ttagctaaaa aaaactctgg ggttgggaaa ggaagataag
     2521 aaacgtattt agcccaataa tctataaagc ttctttgttt cgcaattaat tcaatattat
     2581 ttttttgccc tttattacct gaaacattaa atttaattgg acatcccgtt atcaatttta
     2641 taatatcatc aagattttta ttatctgatt caggaatttt atctgatata acaacaccat
     2701 tttcaagata gttcattaaa ttatttgatt cactaacatt agtgttttga tctccatcaa
     2761 gccaaaaata atggttatcg gaatctaaat acgatgagtt taaaatatta ttacaaatta
     2821 tttgatttgc tccaccagga atatatctca ctactaaatt ctgtttaaga ttctcactac
     2881 ctgaatgagt gataacaaac tctagaatat atttagctag tctatcttca acataaatca
     2941 tcttcctaga atgatacaca tcacctaatt caaaaaatgc atcctgataa tcaatatttt
     3001 caataacatc taccttttct ccgtttttca ctaaaagttt cacggcttct ctaagaaaat
     3061 cttttataag ttgtgtagaa tgtgtagtga taataatttg atgtttttta tttaaacact
     3121 cttgaagtaa aaactcttta aatttataga ttgcactcgg atgaagtgag atttcaggtt
     3181 catctattaa tattaatgaa tttgattgcg catttactat atcatttact aacaaaataa
     3241 ttctagcctc acctgttcct gcaaaagcct cggaatattc ttttccagat tttttcatcc
     3301 aaatagtttt ggaagctttt atatcatcac cttttgaata caacttatgt gttaaaattt
     3361 gaatgtctgt ataagattca tccattattt cactaataat ttcacaaact ttatcatcaa
     3421 ctttaacatt atctataacc atttcctttt tataacgcgt ataactactt gtattattct
     3481 ttaaaatatc agcaactggt ttagatcgta atcttataaa atcttgttta ctacgttgag
     3541 tagaaatttt tttaaaatta tagtgataga aaaataaatc aaaagcagaa acatattctt
     3601 tacaatcaca aaagacaaca tttttttcaa tgccatccca tctgtctgtc gaagaacttc
     3661 caatatattt atttttgggt aatctttcca tctcatattg tttttgagga gcatatggtt
     3721 cccaataatc taatcctttt tttgttccag aacggccttt aagaacttct acatttctag
     3781 aagctttaac gttataatat gaatagatta aacattgttt cccatccact tcatctattt
     3841 gatcaacatt tgtactaaac caatattcag acacactttt attggctgga gaaccatata
     3901 aagcttgtaa aattgaagtt ttatttactc catatctatt acagacacct caggattatt
     3961 taacttataa gttttaacag ctacggaatc aatttcaaca gcaacttgaa catctatgcc
     4021 tgatttttta aggccacttg tagtgccacc tgcaccgtta aataaatcaa tagcaacaat
     4081 tttccccata gtattctcct aaagtttctc ctttttatta taacattatc aaatgtaaaa
     4141 cccaacccga tagggttagg tttttaacat catttcacca acttcttcat ctcatcaata
     4201 cgtactacgg tcacatcata cttggcttgg tagtcggctt gtttgtcttt ttctttttgg
     4261 acaacttctg gtttagcgtt ggcgacgaag cgttcgttgc tgagtttttt gccaaccatg
     4321 tcgagttctt tctgccattt ggcaagttct ttttccagac gtgccaattc ttcttcgaca
     4381 ttcaagaggt ctaccagtgg caagaagatt tcggctcctg taataacgct tgacatagcc
     4441 agttcaggtg cagggatggt tgatgcgatt tccaagtgtt ctggatttgt aaagcgtttg
     4501 atatagttga cattgctgtt aaagaaggct tccaagtcgc tatcgcttgt cttaacaagg
     4561 atggtgatag gtttgcttgg tgctacattt acttccgcac gcgcattacg aacggcacgg
     4621 atcaagtctt tgagactttc cacaccagtg tgggcagcaa ggtcctcaaa ggctgggttg
     4681 acagttgggt atgctgctgt cacgatagag ccttcagcgt actgaccaaa gatttcttct
     4741 gtcacgaacg gcattattga gtgaaggaga cgaaggattt tatccagcgt atagaggaga
     4801 acagatcgag taattacctt atcgtcttca ttgtcgctgt aaagaacttc cttggtcaac
     4861 tcaacatacc agttggcaaa ttcttcccaa atgaagttgt aaaggatatg accagccaca
     4921 ccaaactcga acttatcaaa gttttcagta acttttgcaa tggtttcgtt gagattgtgg
     4981 agaatccagc ggtccgtcac attaccagcc tcacctgttg caacttttgt gacattgtca
     5041 tgcgccacat ccagcgtcaa acctccattg ttcatgagga tatagcgaga aatgttccaa
     5101 atcttgttaa taaagttcca tgaagcatcc attttctcat aagagaaacg cacgtcttgt
     5161 cctggcgcag aaccgtttga aaggaaccat cgaagggcat cggcaccgta tttctcgata
     5221 acatccattg ggtcaatccc gttaccgaga gacttagaca tcttgcgtcc ttgctcgtca
     5281 cgaatgagac cgtggataag gacgttttgg aatggttgac ggcctgtgaa ttccaatgac
     5341 tggaagatca tacgagacac ccagaagaag atgatgtcgt aacctgttac caaggttgaa
     5401 gttgggaaat aacgtttaaa gtcttctgag tcgacttcag gccagcccat ggttgaaaat
     5461 ggccagaggg cagaactgaa ccaagtatcc aagacgtctt cgtcctgagt ccatccgtca
     5521 ccttctggag cttcttcgcc gacatacatt tcaccatcag cattgtacca ggcagggatt
     5581 tggtgacccc accaaagctg acgagagata acccagtcgt ggacattttc catccattga
     5641 aggaaggtat cgttgaaacg aggtgggtag aattcgacct tgtcctctgt gtcttggttg
     5701 gcaatggcgt tcttagccaa ttggtccatc ttgacgaacc attgagtaga caagcgtggc
     5761 tcaactacga cacctgtacg ttctgagtga ccaacactgt ggacacgttt ttcgattttg
     5821 acaagggcac cgatttcttc caacttagca atgactgcct tacgagcttc aaaacgatcc
     5881 atgcctgaaa attcaaaggc aagctcattc atagttccgt cgtcgttcat gacgttgact
     5941 tgtggcaagt tatgacgttg gccaaccaag aagtcatttg gatcgtgggc aggtgtgatt
     6001 ttcacgacac cagtaccaaa ctcaggatct gcgtgctcat ctccaacgat tgggatgagt
     6061 ttattagcga ttggaaggat gacgttttta ccaatcaagt ccttgtagcg cgggtcttct
     6121 ggattaaccg caaccgcaac gtccccaaac atagtctcag gacgagttgt agcaacttca
     6181 agggcgcgtg aaccatcttc cagcatgtaa ttcatgtggt agaaggcacc ttccacatcc
     6241 ttgtgaatca cctcaatatc agaaagggct gtgcgagctg ctgggtccca gttgatgata
     6301 aactcaccac ggtagatcca gcctttcttg taaaggtcca caaagacctt acgaacagct
     6361 tttgacaaac cttcgtcaag agtgaaacgc tcacgagaat agtctacaga gagccccatc
     6421 ttgccccatt gttccttgat ggtagtggca tattcgtctt tccattccca gactttcgtc
     6481 aagaaagact cacgaccaag gtcatagcgg gaaatgccct caccacgcaa gcgctcctca
     6541 accttagcct gagtcgcaat accagcgtgg tccattcctg gaagccaaag cgtatcaaaa
     6601 ccttgcatgc gtttttgacg gatgataata tcttgcaaag ttgtatccca agcgtgacca
     6661 aggtgaagtt tacctgtaac gtttggtggt ggaataacga ttgaataagg cttagccttt
     6721 tgatcgcctg aaggcttgaa aacatcagca tcaagccatt tttggtaacg accagcctca
     6781 acctcggctg gattgtattt agatgaaagt tctttagaca tgtgtgtgtc ctttctctat
     6841 tttgtttatt ttattttgaa tttgcttagc agcttcttct gcagacaaat tcgtattatt
     6901 tattttaaag tagtggtgca actcattcgg ttgatattgg gaatttaatt gaagtgtttc
     6961 agcggtctct ataatttctc tttcagatac ctcaatatgt cgttttaagg gtttgtactt
     7021 taatcgattc tccgttcgat ttcgacgtat gcactcttca agacttgttt ccaactcaac
     7081 aaaaagaatc tcttgatgat aatcatccaa caaactctga atttgcttta aatatatcag
     7141 atgatactga tttgaaaaat caattacatc tgttaaaatt actgatcgct gatttcttgc
     7201 actcgctcca aggaatgaaa aagtgattcc acgaacaaat tcccacattt cctctgtata
     7261 gtcctgatag atttctagtg caaaatcgat ggcttgatgg ttataaaata gggtagcatc
     7321 cgtcagtcga gataattctt gaccaatggt catttttcct gatgctggag caccaatgat
     7381 gaaaagatgc atcaaatcac ctcccattca ctcttcagca aggcatatct taaatcatca
     7441 cagcagttgc cttgagcatc tttgcggtct cttatgcgag cttcgagggt aaagtcaagc
     7501 ttttccgcga ctcgtttact ttgaaggtta tatccaaagc aagttagttc aatcttgtga
     7561 agacccaaat ctttaaaggc taagtcaatc aaggcacgcg cagcttctgg cacataacct
     7621 cgcccccaat agtctgggtg taaggtatag ccgatttcca gcacatcatc ttcatggcga
     7681 tggttgaaat cgacagagcc aacgatctta tccgttcctt tgacgacaat cccgtagcct
     7741 gctgggagat tttccttttg attacgctcc ggaaaaatgt gctccagata ataaatctca
     7801 tcttccaagg tcttgactgg aggaaaacct gctggatagg cgacctctgg caaactagca
     7861 tagtcaaaga tatcctcagc atccgccaca gtacggactc gtaaaacgag acgttctgtt
     7921 tcgattttat ctggcagctc agttcttgcc atccttcttc ctcgcttttt tgatgaaact
     7981 gcccttcata tctacacgct tgtccagata gcaataaacg cgctgatatc catctcccat
     8041 gaaataggtt gggcaaacag ttgattttta aaatgtccct tttcatccag gagttctggg
     8101 gcaacaagtc gctcaagaat cttggcaaag atgtggcaaa taccgtcttc ctcaacaatc
     8161 ctatctaccc gataatctaa aacaagtgga caggcgtcta aaataggagt ctgagttcgt
     8221 tcagaaattt cataatgcac tcccaaacgt tccaatttct cctgatgact gataaaacca
     8281 gcctgctcca tcgcaagcat agaagtttca tcagaaatat tcacagtaaa tttttgatat
     8341 tgtttgatct gctctgcggc attctctctc gcaacgactc caatcacaac ccaatctcct
     8401 agactataag aggaactaca ggtcatgatg ttatagctaa aattctgatc ttgatatcct
     8461 aaaataaaaa caggaaaacc ataatatagt ttattagttt taaatgattg ttttataaca
     8521 acctcctttg actaagacac aaaagaaaag ccctgccatc tacatgacag ggacgaatgt
     8581 gtttatccgc ggtaccaccc aatttcgggc caagcccgca tctttagtct ttaaaataaa
     8641 aagacagaaa tatttcattt tttcagtcat tagcaaccag ttttgacaca tttgtggact
     8701 tctcagcgcc gccactttct gtaaaatgtg ggattcaaaa cacctctaat ggctctattg
     8761 tagcaagttt ttctcggaaa tgcaaggctc aagaaaaatt acttgaactt gatgccattt
     8821 tctttcaatt tcttgatggt agctgggccg attcctttca aggcaaggag ttctttttca
     8881 gtccagtttt tgaaatctga agcagacttg attccttcat catagaaagt cttggcacga
     8941 tcaagtggaa gtccccccaa gtttgctgca aaatcttcta cagaattagc tactttttga
     9001 gcttgctctt tggtagcttc tactgcttgt tcaacttttt tagaaacagc cttaccagct
     9061 gcgcttgctg gctgttctgc acgcttcacg actttttttg tctcatctac aaccttagtc
     9121 acagtacttg aaaaggcacc tgcgcgacgg aggccatttc gtaattgttt tttacgattg
     9181 agtttctttg acatgataaa atcctttcaa taaaaaatcc ctgctctgac aaggatttaa
     9241 cataaacatt ctatcaagat tttagtgaaa aatcaagaat ctttctcgtt taataatttc
     9301 gctcaccaaa caagccttct ggcaaatcat ggaatccctt gcctgctttg aagttttcaa
     9361 acttaccaag caagaactga taagcatcca agcgactctc gtagcgaatc atggcatctt
     9421 tggcacgctc gtcctggtct tcttcgtaaa ctttttgact ttccttgtgc gccatgtcgt
     9481 taatcatgat ttgggtattg acccaagctt caaattcctt cataaattct tgttcgtaac
     9541 tcattttttc tccttattct aatccacgaa aatagtccgt atcaatctca cggtagggct
     9601 ggatatcctg aacatactcc aacactcctt ggaattctcc gtcttcatcg tgtactgcag
     9661 cataggtgat gtggacaaac ttacctcgcg actcagactt gaaccacatt tcatacttgt
     9721 ctttgcttcc ctcacgaagc cccttcatga tagttttgac cttgtccaag tacttaggcg
     9781 gatggcagag ttcgacattg cgcccgactt gagacggcgt ccgtttgaaa atcatctcat
     9841 cagctggcgt attgtcattg taatactgga aaatatcatc tttattgaca aaggtaatct
     9901 ccatagggag gtgattgagg atgagattgg cctgctcgac tgaaagatag ccattaccaa
     9961 aagcctgttg actatggcgg tccagcacag cttccttttc cttaggggta aaggtaatgg
    10021 taaaatggcc ttctggcgta tctatgactt gttgaacttg accttctgcc gtatctagct
    10081 gtacaggctc ctctgcaatc ttttcctcaa taaagctctg tcgttctggc acccatttct
    10141 ctgacggacg gatgatggca tagccatagg catcgctctc ctccgcaatc tgaagccagt
    10201 catcctgagt aaaagactca aggagaatca tgaggaggat ggactcttcc ttgaaaatca
    10261 tactttcaaa ctctgtcgca aaagcttcaa aagcttcctt tacactgcta attgacactt
    10321 ctggtagtga cttggctgtc gttagagctg tttgaaagag ttccctaatc tgatcatcca
    10381 ctccccacat aactttggga ggtgaatcgt gtccatagcg ctccatgata ggaaagaaga
    10441 gttcttcctt acgttggtaa tggatgtcaa attgacccac aagtcccatc tgacgcacca
    10501 aacccttacg catctccgcc agcatttcct cgtcttccat atactcatag gtatctaaca
    10561 atctacgaat gcgaatcaag gccgcacgga gagccagatt ttcttctttg aagacacgaa
    10621 ctgggtgacc tggatgctca gtatctgaaa cttcgacacc tttgatagca tttttaaaaa
    10681 gattggcatg gacatcacag agttccataa catcttcaaa ggtgacacct gagtctgagt
    10741 tcatcagctc gtgctccata agggaaatct cgatggctga gacacccgca aaggtcgcat
    10801 caaagcgctc ttgaaccgac tcaggagagg cgccattgtg caattctaac aaaatatccc
    10861 gtaggacatg aatccgttca tctgccatta gtctaatcca atcacttcgt agccattcgc
    10921 ttccagtgtg cgtacaatct tgtccatagg agttcctgct agcttagaac cctgtttgag
    10981 cgataccttg cgaccaactg tattgcgcat taagggattg gcaaggggtt taaaacccaa
    11041 ctccactaga atttccaaga cttctggatg cttgtccacc acttctgcaa caggaattga
    11101 cacatcgatg atattgtcca tgacgacctc cattgtatgt tctaatactc aatgaaaatc
    11161 aaagagcaaa ctaggaagct agctgcagac tgctcaaaac agtgctttga ggttgtggat
    11221 agaactgacg aagtcagctc aaaacaccgt tttgaggttg tagatagaac tgacgaagtc
    11281 agtaaccata cctacagcaa ggtgaagctg acgtggtttg aagagatttt cgaagagtat
    11341 aaaatgattt tactgcttat attataccat atggagagag attaaaaaac tagcagtggg
    11401 gttcctgcta gttttctgcg acttggatta ccatctagat aagtttaaag aaccaatcca
    11461 atatcttgta aaacagcaat tgaactgcaa aagaaatgat catccaaaag aatttcacaa
    11521 tcccaataat cggtttgata aaaagaatag caagaagacc aaaaaaattt cattctctct
    11581 cctctggctt aatgagccac cgagccgtat ccattagctt atctgcaata aaaagtttgc
    11641 ccagaccaac aacgacatgg tcagctttct ttatcgtttt catcactttt acaccttgca
    11701 cggtcaaaaa gtaattacca taaattttag ctagagtttt tatgagtccc atattactct
    11761 ccttcgatga tttcagcctc ttttcggatt gtttcaacat cttcttgata ttgaacttca
    11821 ctatagttaa ctagcttgga caattctttc tgcaagcttt cacccatcgg tttcatagtt
    11881 gcaaaggtta aaccacctga aatgatacca cccaagatag gaataaattt ccccattcct
    11941 ttggccagcc ctcccttggt aagattcaca ccaaatattc ttaagacttt tttcaaaata
    12001 ggataccaaa gcgtcttagt taaagcttta ttaggtactg ttttcattac ctgtttggca
    12061 attgttatac caccagcacg tagcaaagca gcaattccat tcacccccaa catgacgcct
    12121 agataaagca agagggtatt ttgagcatct tcactcaact catcgcgtga agcccaaaga
    12181 tcctcataac cataaatata acctaattct tgggccaatt tcagagagaa agcataaaat
    12241 tgagccacat cagctggaat ggtaattgcc atagcaagcc ctccaggtaa tcctgccaaa
    12301 acagaagtcc cactcgctaa taaaacatta tccctaatac aggcattagc cactcgatct
    12361 aagatttctt gagataagag agacgttgga ccttgttcca ataaggtagg aatgtcctgt
    12421 ggacccaatt ctttggaaaa cttatccact aaaaatttcg aacgatcaac cttaacaact
    12481 ggtagtttta ccacttgttg taatacttgc atagctaagt cttgttcagc catatacaaa
    12541 ctccttattt cttttttaca ccttatcata ttatatttcc tacaaaaaaa gcattttcta
    12601 attagataac attaaaaaat acagaacccc tttttttata gtaaaatgaa acacgaacag
    12661 gacaaatcga tcaggacagt caaatcgatt tctaacaatg ttttagaagt agaggcgtac
    12721 tattctagtt tcaatatatt ttaaaagaat atttttccat ttcaaattca acctattttc
    12781 ctccaccaaa aaagaggggc aacccctctt ctttagttct tatccagatt gcgtagcatc
    12841 tcgtcaatct tgtttccata ctcgatggac tcgtctttga cgaaggtcaa atctgggatt
    12901 ttgtacaatt tcaaattgcg accaagttca cgtttgatgg taccagttgc tttttcaagc
    12961 ccgatttggg ctttttggtt atccgaagca aggttactca aaatggtgta ataaaccttg
    13021 gcaacagaca agtcacccag catctgaaca tctgtgatgg tcacaccttg gacacgtgga
    13081 tcacggactt tcttttgcaa aatctcattg acttcacgct tgatttccat gcccacacga
    13141 tctgtacgga aatgatttgc catgatattc ctttctctac tttgaaccaa aagctaggcc
    13201 agcagaccta gctattttaa acttcttgat tttcagttta aattgaaacg aagttcaatt
    13261 tgaagtcatc tgcttctttc gccgtggtga aagtgatgga actgatttat cagtcccatc
    13321 acaggggatt tttgagaccc taggctcaaa aataagcaat gaaaccatcg gtttgcttgc
    13381 gtccctcacc acctaagaaa ggagcaaaaa atcttatctc ttgatttctt ccatgacata
    13441 cgcctcaatc acatcatcca tcttaatatc attgtagccg tcgatcatca atccaccttc
    13501 acggccgttt gtaacttcct taacgtcgtc tttatagtgt ttcaagcttg cgagttcacc
    13561 atcatagata acgacaccat cacggataac acggacttta gagtcacggg caaccttacc
    13621 gttgataacc ataaatccac cgatagttcc cactttagac accttgaagg tttcacggat
    13681 aactgcttca ccaataactt tttcttcaaa ttctggatca agcatccctt tcatagcttc
    13741 ttccatctct tcgataacct tgtagataat gctgtgaaga cggatttcca catcgtcagc
    13801 ttctgcttgt tgacgagctt gtggtgtagg gcgtacgttg aaaccaacga taaaggcatt
    13861 tgaagcttcg gcaagggtca cgtctgattc gttgatagca ccgaccgctg agtggacgat
    13921 agtcactttg acaccttcca cgtcaatctt ttgaagtgag gcagaaaggg cttcaacaga
    13981 accttgtaca tcagccttga tgataacatt aacagatttg agttccccag ctttaagggt
    14041 atcaaagagg ttttcaaggc taacacgttg ggtagcttga cgttgtttca tgagggcacg
    14101 tttggcacgc tcttcacctg ctgcacgcgc agatttttca tcctcgtaaa cggcaaagtg
    14161 gtcacccgcc atcggtgctt cgttcaaacc tgtgatagag actggtgttg atggtccagc
    14221 aactttaaca cgacgaccaa ggtcgttggt catagcacgg acacgaccga aggtatttcc
    14281 gacaacgatt gggtcttgaa cattcaaggt accttgttgt acaagaaggg ttgcgaccgc
    14341 accttttcct ttatccaagc gcgcttcgat aaccgtaccg attgcacgca ctgttgggtc
    14401 tgccttgagt tcttggattt cagctacaag aaggactgtt tccaacaatt cttcgatatt
    14461 ttggttgaat ttagctgaga tttcaacaaa ttcagaatct ccaccccaag cggttgacat
    14521 cacaccatgc tctgccaatt caccgataac gcgttctggg ttagcacctg gtttatcaat
    14581 cttgttaatg gctacgatga ttggaacgtt agctgctttt gagtggttga tggcttcgat
    14641 agtctgaggc ataaccccgt catctgccgc tacgaccaag atcgtaatat cggtaacaga
    14701 agcaccacgc gcacgcattg atgtaaaggc cgcgtgtcct ggtgtatcaa ggaaggtaat
    14761 cttcttacca ttttccacga tttggtaggc accgatatgc tgagtaatac cacctgcttc
    14821 acctgtcgca acacgtgagt tacgaagagt atccaaaagg gttgttttac cgtggtcaac
    14881 gtgtcccatg atagtaacaa ctggtggacg ctcaaccaat tcatcttcat tgagataacc
    14941 atcttcgaca aagaaacgtt cgatgtcagc attatccact tcaacctttt gtttggcttc
    15001 gataccgtaa tccaccatga ggagttcaat tgtttcccca tccaaggatt ggttttgtgt
    15061 ggccatgaca cccatcatga aaagtttctt aacgatttca gctggttcac gtttgatacg
    15121 ttttgcgatt tccgcaacgg tcataccatc tgtatattca aattctgttg gcaattcatg
    15181 gaatttacgc tccgtaacag gttttggagt ctgattacgg ttgttcttgt tattgccttt
    15241 tttgttcttt ttgttgttat tccagttact attcttttga tttctcactt gattttgact
    15301 acttcgattc ttttgttgtt ttctaggacc atcttcttca tgatcataat cgtcacgatt
    15361 tttgtctggt cgagcttgtt ttttacgacg tgtatccact gcaggttctt ttttctcagg
    15421 gacgacttca accactgctt gaacttgctg tgcttgttca gctaacttag ccgcttcttc
    15481 aaagatttcc tctggttcct tgcgtttgtt agcttgagcc aaggcttctt tagcagcctg
    15541 atactgcttg aagcgttcct cacttgaacg agcgtactct gcattttgct ctgcttttag
    15601 ggctgctgca cgggctttaa agtcaatacg tggagccgct tgatttgaac gtttatcctc
    15661 ttgctgacgg cgttcatttc tacgtttttg ctgaccttgc tgcttcttag cttggtcatt
    15721 aaagcgacga ttgtcgcggt tgctttgacc ttgttttcca ccattacggc cgtcgttttt
    15781 ctgacggttt ccgttttgtt gttggtcacg gttattgccc ttattttgct tgcgtcgctc
    15841 tgcctgctct ttagcacgtg cttcacgctc agccttgaaa ttacgacttt gcggtttagc
    15901 cgctactttt tctgttttcg gagtgactgg ctcagctggt tttgcctctt ccttggctac
    15961 agctggtttt actggttcag acttttcggc tttcttttct gcacttgctt ttggggctgc
    16021 aggttttgcc tcttccttag ctacagctgg tttagctggc tcagattttt cggctttctt
    16081 ttctgcactt acttttgggg ctgcaggttt tgcttctact ttcggagcag ctgcaggctt
    16141 aaagctggca gcaatttttg cagcgacagc ttcttccaca cttgatgagt ggcttttcac
    16201 atccaagccc aactcttttg cacgcgctac aacttcttta ctttcttttc caagttcttt
    16261 tgcgatttcg tacaatcttt tcttagacaa atcatgtcct cctcttctat tccataagag
    16321 acctcatttt ctttgtaaat ccagcatctg ttacagccaa aacctttctc gatttcccga
    16381 ctgctatgct taattccagt gttgaaaaca cggttacaat ttctacttga taataatgac
    16441 ttttatcttg aatcttcttg gtcagattgg gtccagcatc atgagctaga aagaccaact
    16501 tggccttgcc gtcttgaatg gccttgacca ccaattcttc acccgatatg atgcgccctg
    16561 ctcgctgagc aagccccaag agattactta tcttttgctt attcaagtcc caactctctt
    16621 cttttcactt tgtgatccac ataagcgatc aactcgtcat aaaagctttc ttccacttcc
    16681 atgctaaagc tgcggttaaa gaccttcttc tttttcgcct ctagggcttc tgcattgtct
    16741 agtttgatat aagcgccgcg gccattggcc ttgcccgtag gatcaataaa gacttgtcct
    16801 tccttgttct tgacaatgcg gagcaaatca cgcttatcaa tcacttcgtt agacacaaca
    16861 gacttgcgca aagggatttt tcttgttttc atctttccct cctctagcag cttttattct
    16921 tctacagtat cgttttctac ttccaactct actgaagcag cgtcttccat ggcttcaaat
    16981 tcgctagcag acttgatatc gatacggtaa ccagtcaagt gagccgccaa gcgcacgttt
    17041 tgtccacgac gaccaatggc aagagaaagc ttgttatctg gaacaaccac caaggcacgt
    17101 ttgctgtcgt tttcatcaaa gataacttgg tcaacctcag caggagcgat ggcattgtag
    17161 ataaattcag ctggatctgc tacccactcg ataacatcga tattttcttc gattggtacc
    17221 atgcggtcat ttttagcatc gtaacgagct gggtggaatt tgctagtaat cttcttgata
    17281 ttagcaccac cacgtccaac gattgtaccg atagcatcca cgtttggatt gtggctacga
    17341 acagcaacct tcgtacggtc acctgcttca cgagccacgc tcatgatttc aacagttcca
    17401 tcataaactt ctggaatttc ttgctccatt aaacgtttga tcatttctgg atgactacgg
    17461 ctaacaaaga cgttcacacc acgagggttg tcttcaacct tgtaaacata aacttcgata
    17521 cgatcatgag aagcaaaaac ttctccagga atttggtctt gttttgacaa ttgggcttcg
    17581 atgctaccaa ggttgacata gataaagcgg ttgtcaaagc gttctactgt accagacatg
    17641 atttcttgct catgttcttt gtaagtattg taagtgatgg cacgtgtttg cttgcgcatt
    17701 ttttccatga tggtttgttt ggcagattgg gctgctacac gaccaaactc agctggtgct
    17761 tcttcaaact tgattttgtc tccaagttca taagctgaat taatggcaag agcatctttc
    17821 aagctgattt ccaaacggct atcaaatact tcatcaacaa cttcacggac agtataaact
    17881 gtaaagtcac ctgttttttc gttgaagtca atagctacgc tgtctgactg accatagcgt
    17941 ctgcgataag cggaacgaag cgactctact actgcgtcga tgatatcttc ttttttgatt
    18001 cccttgtctt cttccaaaat gcggaaggcc tctagcattt ctttactcat gttttcttca
    18061 cttttgaatc ctcaaaagct atcctttctt tttctataat ttaactgcta aacgtgcttt
    18121 tgatactaaa ctgtatggaa tttggacggt tttcttacgc gtcttgtcca tatattccat
    18181 agtcaactcg tcctcttcga aggccaacaa ggttccttca aagacctttt gcttatcgat
    18241 ggcttggtag agcccgacat ggatgtattt tccaaccgct ccagcgacgg catccttggt
    18301 tttcaaagga cgttccaaac ctggactggt aatttctagg aaatattgtt ctgggaaggg
    18361 atctggcttg atggtgtcta ggacaggact gataatttct gtcaagtctg ccgtgtcgtt
    18421 caaggtaatt ccttcgggtt tatctacaaa aatactgaga atcatgtcac tgccaatctt
    18481 tccatactcg atatccacga gttcaaaagg agcttctatg acaggttcta caacttctct
    18541 gactaattct acgattgttg cgattgcgtc cacctcctca taagcaagag gcgaagatat
    18601 ttccccgcct cacttttcat attcttacta tcagtatagc acgtttgcca acttatgtaa
    18661 agcaaaagca cttgtacagc ctaattttag gttatctctt aaaattctgc ctcaactcgg
    18721 tagataactt gccccttgtt tgagaatttt tgctcgtatt ctgtcatgac attgccttca
    18781 aaatcactgg catgcaaatc taaccagaca ccattgagtt tcatgccata ttgagaaaag
    18841 ctcactaaac tgtactcaaa caagccacgg ttatccgtct tgaaatgaat ttctccattt
    18901 tcaggcaaga tacgtttgaa ggtatccaag aaggtcttgt aggtcaaacg acgcttttca
    18961 tggcgttttt tcggccatgg atctgaaaag ttcagataca agcgatcaat ctcaccgtct
    19021 tcaaagtagt cagttaagtc agaaccatct acccacaaga gcttgatgtt aggcactcca
    19081 acttcaagca ccttgtccaa agcgtagctc aaaacagact tttgaatatc aatcccgata
    19141 tagttgatgt cagggttttg cttggccata cctgaaacaa aggcaccctt tccacttcca
    19201 acttccacat gaatgggatt atcattgcca aacaagtccc gccattttgc cttggcttcc
    19261 aagggattga ggaccacata ctggggattt gcctctagta attctgttgc ccctttacga
    19321 tttctaactc tcatcttctc tttccatact tgtctcggaa atgacgcaaa ccatgaatct
    19381 cccgattgac attttctaaa tcttggttca tataatactt ggaaatctga ctcaaataag
    19441 acaattgacc ataccaatac aatttactta ataccgtttg attgtacttg taaccgtagt
    19501 aggtcaacca ttccttccac tgatgttctg aaatataatg gcagagcata tgggccacat
    19561 caaacatgcg atcggtcaag cgaaccgaat cccaatctac taaataaatc aagccactat
    19621 ctgtctcaat ccaattacta tgtcgtacat ctccatggac aatggtcgca tggtcctctc
    19681 taaatcctgg aatagtctga cgtaaatcag ccatcacttc actgataaaa tgatttttac
    19741 gcaaagcatc tggagccgtt tcctgccaag actgtagtaa atctacaggt gtttccatgg
    19801 catagcccaa acgactcaat tgtgtcatca acggacgtga gcgatgaaga cgggttaaaa
    19861 tattgacgat ttgcttacga ttcatatcat agggggtcaa tatcttgcct gtcaaccatt
    19921 cttgagcaca catatcacgc ccatctgcca aacgacgact ccataataat tgtggagcaa
    19981 tttgttctct agctagacca ggtaggattg gagaggtgtt catttttaca aagatgcgct
    20041 tcccatcagg atagctaccc atataagcct tgccactttt cccaggtatg ggagtcagtg
    20101 ttagctcatt atcacccaaa tccatttctt tcctccgtat aaagtttcct tactattcta
    20161 ctagtttttg gcctattcgt caaccactct aacccgattc tcaaagaaaa gcctctggag
    20221 tggtttgggt tatcattata gaaagaaaaa ggtaagcaca atctcctact taccttttca
    20281 tgatttttat aaataatata agagtggcca actgttgtaa agcatatgga ctagagtaga
    20341 aacccatagg ttttggctct ttttataagc aaagcccagc aacaaacccc caagtagata
    20401 aaaaataaac gaagggacat tagaaggtcc atgcatgaaa gaaaagagag aggaagttag
    20461 cacaagccct aaccaagaat tgtcaaaaac cgcaccttga agaagtcctc tgaaaatgag
    20521 ttcctcctga attggcccaa aaactgaggt agtcaaaatc aaagacaggg aaattcctct
    20581 gctgacttcc gccaagtgtt gaactcctgc gccagaagaa acagcgaaga aatgaatata
    20641 aaccagcgtc tcaagcacac ttagaagaaa gattgcaatg aaaagcaaaa gttgtttctt
    20701 gcttaacatc ccaaaagaaa tcatttcaaa ctttctagca taagcaacac tcagagaggt
    20761 tactagaaga cttacaataa tcaatgcaga ttcattttga aaaaaatccc catggttaat
    20821 cgtataagga actgtaatga cgattattat tactaaaaat ccaaagcaca aggcatgaaa
    20881 tttatcaaaa aactccataa aattatcctc ctcaccaaac agacttccta cgcgtcatcc
    20941 tttagctcta acctttccaa aacaaaccat tttagtagcc aaaatccgac cacatagcca
    21001 gcccctaaga atatagccat taaagctagc atggaattta ggaaattaaa aaccaccgca
    21061 gatacaaagg ttagcacaaa aacattaaaa gcaatggtgt cagaagccaa gactagaata
    21121 tagggtgtca accgatctaa agttttggaa tctaggaaaa ataagtgttt atacatgatg
    21181 acctcctcta tggctgaaaa gcaagccttt tgttttttta ccccaagacc ctatgtagaa
    21241 aagtgagcaa aaacgggaag gtcgctacaa tattattgat cacatgcacc gcataggatg
    21301 gataaatgct cttggtatag cgggtcaaac cagcaaagat gattccaact gttgcaaaga
    21361 cgaagatatc taacagacta ggcagactca aaaaatgagg gagagcaaat aaaatagaag
    21421 gaagaagcaa atcaagacca aatcgcgaat gcttaaagaa agcatgttgc agtaatcctc
    21481 tataaatcaa ttcttccatc agtggaacca gaaagaacag ggctatataa atacctagct
    21541 ctgcaaagtt agtcccacta taaccaatca atacagccca accttccgca gttgactgaa
    21601 catgtttagc tgtctgaacg ttaaaagaga tctggaacac taccactaat actgtcaaaa
    21661 tcgaatacca aagccatttt tttcttggaa tgcggaagag ataaccatgg cctgtcttaa
    21721 caagaaccac aatcatgact ccaataaaaa gtaaactcaa gatattttga atccagaata
    21781 aattgcctat ctgagaagaa aattgccaat agttttggac gataagcgtc agctgagaaa
    21841 gaccaaatac gaaaaataag taagagaaga ctgcacttat tttgaataga agttgatact
    21901 ttttcataaa aatcctccct actatgacct caccttgtca ggctctactg ctgtaagatt
    21961 aagaagacag tttgtttttt ttaaggctaa cctgactact agataataga tacattaagg
    22021 cattaaagac aatgaaaata tgtccataga ataaaatcaa cctcgcatcc aaaccaagat
    22081 aaagtttgat tatcaaaaag atgagcaaaa gaatttgaaa ccataaggtt tttccaaaaa
    22141 taaatttaaa gcgttttcga atatctactt ccttgatttt taccgccacc cctttattag
    22201 caagaaggaa aactcctgct tcaaacaaac cactgtaaag aacaagccac ccaatagata
    22261 cgatagagat ttgtaaaaat gtccctaaaa gaatatccaa cacactactc aagaaaataa
    22321 caaaaaataa tctgtatttc atattaaata cctccattca tttatttcac taacaattta
    22381 atagagcctt ctactcaaat atcctgtcag aaaaggatag aaagctactt tttataatac
    22441 ttcaagcccc acatgagcag aagcgtgata aacaagcaga gaatacacct atataagcga
    22501 ttagttgttg atagaattct gtttctgaaa tacctctata caaacaaatg acaaacataa
    22561 aatctgccaa gccgataaac ataagttgat tggttctagg actaaccaaa tcatcattta
    22621 cttatattta agagtatctc ttttatgtta gtgtatgtta acactaaaaa acaagttagg
    22681 ccaataatat ttaaaatgaa caataacggg gttaagtctc taaaaaaatt atctactgac
    22741 actacaagaa atactataca tattatagtc gaaactatct ttttcttatc cataattatt
    22801 tactcctttc ctaacaaatc caacttatca atcaagagcg atttttaaca taatgtagca
    22861 gcacccgttg caactttgac aagtttagta tatcattgtt ttttaaaatt tttcatccaa
    22921 atcttgaatt gtcatcgaaa cctcctaaat tgcaaaaact tcattagccc ctacttttta
    22981 taatacttca aaccccaaat gagtagaagc atgatgatca agcaaagaat agcaccaata
    23041 taagcgatta gttgttgata agattctcct gctgtgatac ctctatacaa acaaataata
    23101 gacataaagc ctgtcaagcc gataaacatg agttgattgg ttctaggact aaccaaatca
    23161 tcgtcttcaa actcttttat ccgcatttcc ctagtgaggt aaacagtgac caaaatagaa
    23221 gctaagttaa taaccactaa aagaaattgg aaaactgacg aaatagaaat agataagtag
    23281 aaacaagcaa aggcaactgc cccaggaaca atctcacaaa gaatatgatt ctttttaatc
    23341 aagaaaatct ttcatttctt ttctttcttt ttaattaaag aaaaatagat cataaccgca
    23401 atcatatagg ctatggtata aaatagatta taccaaatac tctccctaag cggataaaga
    23461 aagatggaca tgatcagata cagaacgaaa ataatcagta tttttttctt cataagattt
    23521 cctcctaaat gtgcgattta tcttagttga gcaagaacat ttacactgct agtatagcac
    23581 ttattttgac cttggatcac tcaaatcata aatggtcatc aaaacctctt gaattgtaaa
    23641 aattaaaaaa agcaagcatg aaaaacatac tttcctcttt atgttgtatt gataccaact
    23701 tgtttgtaga cttttcatcc tgctatcaca tatcattttg acaggcgaaa caatattaaa
    23761 gaaactcccc tgtaaattaa gctagcaaat acaggggaga aatttatttt ttagagagta
    23821 ctatccgtat cctttttgga agattttgaa aatatttttc taattaagtc atccatataa
    23881 ggaccaaata taccaactac taaaccaata ataaaacttt taaaatccat aattaccacc
    23941 aacatgttgc tacataggct acacctccaa gtatagctcc acctgcagca ccagttacac
    24001 ctattcctat agcaaatggt cccaatagaa atgtcaaacc gttgttgcac acccatcaat
    24061 tgcgccatat gcaacccctg ctgcacaact aatttttctt ccccaatcaa tatctccacc
    24121 ttcaacgcaa gcaagcattt cattatccat aacagaaaat tgtgacatca tttttgtatt
    24181 catatagtat gtcacttttc agttacggaa caagtttaat ataaaaatta tcaaaaaaac
    24241 ataggcaata aagagaaaaa ttaatttatc atagattaga aataatatga caaaacaatt
    24301 caatgatgtt aattcaatag tcttttgttt tttatcagag atacttatgg atagataaat
    24361 aagataggtt tgaaaagcga agagaataat aaagaatata gccttcataa aatttagctt
    24421 tcatttttat gatgtagcgg tataggctaa atatccacaa accactgctc ctccaattcc
    24481 tcctattgca gcgccccatg gtcctagaat tttcccatat ttcactccac ccgctgcaca
    24541 acctaaagca gcaactacag ctgctcctcc ggaattacct ccataaacct cactcagcat
    24601 tgtttcattt atattacaat aagtattcat acaagtctcc ttttattaaa atccacccgt
    24661 tgcccctgtt actcctgccc aaagatccac accaaattta gctcctatgt atccacatgc
    24721 tcccataaat ggtgctccaa caccactcgc agcacaaata gctgtcccta gcccccagcc
    24781 accaaaagca gcaccaccac cttctaagac attagtttgc caattattct tgcctccttc
    24841 aatactagat aacatagtta tatccatttc atgaaattgt tccatcattt ttgtattcat
    24901 gacaaatact cttttttatt tttaattttt gtcttgttgt aactttgaca agtttagtat
    24961 atcatcgttt tttaaaattt ttcatccaga tcttgaatag tcatcgaaac gtcttgaatt
    25021 agcttttcat ttgaaaccac ctctaaattt tttaaaaaaa gataatttct aattattttt
    25081 taccattcag gaagtttcaa tgactattca agatttaata aaatatgaac ttatttttat
    25141 ggcataatat acctatctac tatatgaaag gaattgccaa tgacttctta taaacgtaca
    25201 tttgttcctc aaatagatgc gagagactgc ggtgttgctg ccttagcctc gattgctaaa
    25261 ttctatggtt cagatttttc tctagctcac ttgagagaac ttgcaaagac caataaagaa
    25321 gggacgactg ctcttggcat tgtaaaagcc gctgatgaaa tgggctttga aacaagacct
    25381 gttcaagcag ataaaacgct ctttgacatg agtgatgtcc cctatccatt tatcgttcac
    25441 gttaacaaag aaggaaaact ccaacattac tatgttgtct atcaaacaaa gaaagactat
    25501 ctgattattg gtgatcctga cccttctgta aaaatcacta aaatgtcaaa agaacgcttt
    25561 ttctctgaat ggactggagt agctattttt ctagctccca aacccagcta tcaaccccat
    25621 aaagataaaa agaatggtct actaagcttc cttcctctga ttttcaaaca aaaatctctc
    25681 attgcttaca ttgttctctc aagcttattg gtcactatta tcaatatcgg tggttcttat
    25741 tatctccaag gaatcttaga tgaatacatc ccaaatcaga tgaaatcaac tttaggaatc
    25801 atctcagttg gtctggttat cacctatatc ctccaacaag tcatgagctt ctccagagat
    25861 taagcattga tgtgatttta tcctatattc gccatatttt tgaacttccc atgtctttct
    25921 ttgcgacacg tcgtacagga gaaatcattt cacggttcac agatgctaac tctattatag
    25981 atgccttggc ttctaccatt ctttctcttt ttctggatgt ttctattctg attcttgtag
    26041 ggggagtctt gctggcacaa aaccctaatc tcttccttct ttctcttatt tccattccta
    26101 tatacatgtt catcatcttt tcttttatga aacctttcga aaaaatgaac catgatgtca
    26161 tgcaaagtaa ttctatggtt agctctgcca ttatcaaaga tatcaacggg attgaaacta
    26221 taaagtcgct cacgagtgaa gaaaatcgct atcaaaatat agacagcgaa tttgtagatt
    26281 atttggaaaa atcctttaag ctcagtaaat attctatttt acaaacgagt ttaaagcagg
    26341 gaacaaaatt agttctgaat atccttatcc tatggtttgg cgctcaatta gtcatgtcaa
    26401 gtaaaatttc tatcggtcag ctgattacct ttaacacact tttttcttac tttacaactc
    26461 ctatggaaaa tattatcaac ctccaaacca aactccaatc tgcgaagatc gctaataacc
    26521 gtttgaacga agtctatcta gtcgaatctg aatttcaagt tcaagaaaac cctgttcatt
    26581 cacatttttt gatgggcgat attgaatttg atgacctttc ttataagtat ggttttggac
    26641 gagatacctt aacagatatt aatctcacga ttaaacaagg agataaggtt agcctagttg
    26701 gagttagtgg ttctggtaaa acaactttag ccaaaatgat tgtcaatttc tttgaaccct
    26761 acaaagggca tatttccatc aatcatcagg atattaaaaa cattgataaa aaagtcttgc
    26821 gccgtcatat taattaccta ccccaacaag cctatatctt taatggctct attttggaaa
    26881 acttaacctt gggcggtaat catatgatta gtcaagaaga tattctaaaa gcttgtgaag
    26941 tagctgaaat ccgtcaagac attgaaagaa tgcctatggg ctatcaaact cagctctctg
    27001 atggagctgg tctatcagga ggacagaagc aacgaatcgc cctcgctcgt gctcttttaa
    27061 ctaaatctcc tgttttaata ctagatgaag ctactagcgg tcttgatgtc ttgactgaga
    27121 aaaaggttat agataatctt atgtccctaa ctgataaaac cattctcttt gtagcccatc
    27181 gtctcagtat agccgaacga actaaccgtg tcattgttct tgaccagggg aaaatcattg
    27241 aagttggtag tcaccaagag ttaatgcagg cgcaaggctt ctaccatcat ctattcaata
    27301 aataaggaga atgtcatgaa tcctaatctt tttagaagcg tcgagtttta tcagagacgt
    27361 taccataact atgcgacagt gttaattata cctctttcat tactgtttac tttcatcttg
    27421 attttctccc ttgttgccac aaaagaaatt actgttactt cccaaggaga aatcgcccct
    27481 acaagtgtca ttgcatctat tcagtcgacc agtgataatc ctatccttgc taatcattta
    27541 gtggcaaatc aagtagttga gaaaggtgac ttactcatca aatactctga aacaatggaa
    27601 gagagtcaga aaactgcctt agaaactcaa ttacaaaggc ttgagaagca aaaagaagga
    27661 cttggaatct tgaaacaaag cttagaaaaa gcgactgatc ttttttctag cgaggatgag
    27721 tttggctacc ataatacttt tatgaatttt actaaacaat cccatgatat tgaactgggt
    27781 atctcaaaga ctaacactga agtttcaaat caagctaatc ttaccaatag cagttcatca
    27841 gccatcgaac aagaaattac aaaagttcaa caacaaattg gagaatatca agagctgaga
    27901 gatgctatcg taaataaaag agctcgctta ccaactggca atccgcacca gtcaatttta
    27961 aatcgttatc ttatagcctc tcaagaacaa acacaaggaa cggcagagga gccattttta
    28021 tctcaaatta atcaaagtat tgcaggtctg gaatcatcta tcgcaagcct caaacttcag
    28081 caagctggca tcggaagtgt agcaacttat gataatagtt tagcaaccaa aattgaagta
    28141 ctccgcactc agtttttaca aacagcctca cagcaacaac taaccgtgga gaatcaatta
    28201 acagaattaa aagtacaact agatcaagcg acacagcgct tagaaaacaa taccttaacc
    28261 gctccaagta aaggtatcgt tcatctgaac agcgaatttg aaggtaaaaa tagaattcca
    28321 actggtacag aaattgctca aatattccct atcatcacag atacaagaga agtactaatc
    28381 acttactacg tatcttctga ctatctacct ctactagata aaggacaaac tgtaagatta
    28441 aaactggaga agattggaaa tcacggcacc accatcatcg gccaacttca gacaattgat
    28501 caaactccta ccagaacaga gcaaggtaat ctctttaaat taaccgctct tgcaaaacta
    28561 tctaatgagg atagtaaact catccaatat ggcttacaag gtcgcgtcac tagtgtaact
    28621 gcaaagaaaa catattttga ttatttcaaa gataaaattt taacccattc tgattaattt
    28681 tcagataaca ctctataact atttattatc ttatcaaaaa ggagaatcat aacatggata
    28741 agaaacaaaa cctaacttca tttcaagaac taacaactac cgaactcaac caaattacag
    28801 gtggaggatg gtgggaagaa ctcttacatg aaacaatttt aagtaaattt aaaatcacaa
    28861 aagcacttga actacctatt cagctataaa aacaagaccg agaaacaaga actctcggtc
    28921 ttgtttttta tcattctgca tgtatcacag taagtacctg acgaaagact tgattttggc
    28981 aggtagtatt tagattggta ttgggatgac tttccacaat tttcataacg gtatagagac
    29041 caactcctct ctcctcccct ttagaacttg ctccaaagga gaagatttca gaaatatcga
    29101 tgccctcttc tttgatggag ttttcaatga taaaagtctc ctgtgctcca ttttttaaaa
    29161 aggcgattga aacatgaggt tgactggcct ctgcactagc ttcaatagca ttgtcacaaa
    29221 ggatagacac aatggttaga aaatcaagta ggctcatccc ctcaacctga atctcctcag
    29281 gaacttcaac attaaagaca atgttctttt ctctagcttt tataaatttt ccagctagga
    29341 gactcttgag ggcacggtca cgaatattca ccaatctgcc caggtcatat ttattgtcct
    29401 gcaatttctg actggaatcc cttaagaccg agtcgtagat ctcttttatc tgctccatat
    29461 cctcctcttc aatgcccaaa cgtaagctgg ttaagaggtt agtgtagtca tggcgaaaac
    29521 tccgaatttc cttgtaaagt tcctctatat gccgactata gcgttccata tctctgtagc
    29581 gcaaggtctg ctcttggttc agttcctcct gaagtttttc cttcaaatag gtatccaatt
    29641 tcttgatacc ccccataaaa aagagtaggt aaaacactag gatgagatgg cgaacagtcg
    29701 ttgattgaat accttgttca tattcaaggt aagatagact ttgcatcact agataataag
    29761 cccccattgc ccagttaatc ttagtaagag acttttgaaa acctgtatct agaaactccc
    29821 ttctcaatct agtgaaatca tagtctaacc atttcaaaaa gactaaaact atgaagcaaa
    29881 cgaatatcat aatcaataaa aagataggat tgccatcccc atctacaatt ccttgcccca
    29941 aaaatggaag cacaaaatag gaaacacctc tgtaaaagag ctctactaat atcattggga
    30001 agagaccata aaaaataagg atttttttag gaagccctct taacaataag aaagatagac
    30061 ctataccgaa caaaggttct ataaaatagg acaaatattt acttacagcc aaaaattgaa
    30121 aagtcgtaaa aatgatccct agaagaaatt tcagaagaaa ggccttaaaa atcctctcga
    30181 aagtaagatc aattccatcc accttaaaga agatgacaat ttctagtcca ttagtaacaa
    30241 gtgtatacaa caatatccaa gcaatgttca taaattctcc tagctcagtg taatttattg
    30301 atggcctcag acacttccct gaccttataa cgggcgatta gacaacttcc accattggga
    30361 aagaaaagca gtttttcttt cttatccaaa tgcaccacat ttgcaggatt gatgagaaaa
    30421 gagcggtggc actgcaagag acggggctcc tgcttgaaaa cctcctctaa actcgctgta
    30481 aattccagcc tgtctgtctt ggtatagaga ataacacgat gggctctggg cgacgtttcg
    30541 agatagtaaa cctctttaaa aggatactga aattgggcaa attttgattt aaagtaaaag
    30601 caatcttccg ccagactttt actatcttga ctattggcat agaggagggc tgtctcgatc
    30661 cgagattcaa actcctctgc tgacaaggcc ttatcaatgt agtccaaagc agacacttgg
    30721 tagcgaaaag acaggggcat aaactccgag tgagtcgtca caaagacaat cagggcataa
    30781 ggatcccgat cccgaatctt tctagccact tccagtccct tcatctcttc atttcgaatc
    30841 tcaatatcca aaaagaatag ctgatgggcc cccttctcat gcacttcagc cagcagttgg
    30901 tccggcttgc caaatacctc aaaagagcta ggaatgatat gatgtgcttt caaaagtttc
    30961 tcaatcgtcg tttcaattct agtctgttgg gaaaaatcat cctctaaaac aaatattctc
    31021 atcttcttac tctccctctt tcaaccattt ttgacgaatt tctctatagc gacgtctggc
    31081 atacttgaca gcgtattgac cttcttctcc gagaaaaagg attctttctt gaaaatcaat
    31141 cgattttaat ttatcaagat taaccaaaca atttcgatga catctcatca aggtttcccc
    31201 acattggttc tcaagattac ttaaattttg aagcatatta aaactagctt cttctgtaac
    31261 aatctgtacg gtatgggctt tagttggatg tgtttggata tagtagatat cgtcgatgtt
    31321 tactttatag actgttttct gcgtctgaat aatcatatat ttcattggat tctcctccta
    31381 agaaaagaat aacagaaaag gtctagccca agcacaccca atcctaaaca gtctttgaaa
    31441 actcctaaaa ggattatttt ctacaacatc atgatgagct tacaactatt ttatcataaa
    31501 attcactcag cgcaattcaa gacatttcaa tgacaattaa agatttgaat aaaaaaaaag
    31561 aattaaaagt gataaactaa cagtatcaaa acacagttgc cagaagtaag actggcaccc
    31621 agattaaagg aggcattccc atgacagata cagaccccat caaaagagct catactttga
    31681 ttactgactt aaacaaagcc taccaagcat gcaaacaggc aagcgctgac gacgtccgct
    31741 ttcaggagca attaaactct attcttggtt ttctagccaa ggctgaaaca gtggataatc
    31801 gattcttgat tgaactagaa aaattttacc agacttccag tcttctcatg ggactcagcg
    31861 ctcttgatcc agatgctcca actcgcgccg cttggcgggc ctatgaccgc ttccactttg
    31921 accaagtcaa gaccaagtta atactcaatg aaaatcaaag agcaaactag gaagctagcc
    31981 gcaagctgta cttgagtacg gtaagacgac gctgacgtgg tttgaagata ttttcgaaga
    32041 gtataagtct ctacggacca accattatcc tgtagtaaca aaaaagaagt tgagacagac
    32101 tgaactcaac ttctttttta gtgtcgtatc agcaatgtta gtcctgcatc tgacgtttta
    32161 cctgataagg caaatacaag actagtaaaa ccaaaccagc tcctagtaag gctagaaggg
    32221 ctagtttttc ttggaaggta atcaacccaa caactaattc cacaagtaaa acaaaactgg
    32281 tcaatcctcg aactacctcc tgtaagcctt tttccttttg ccccttgtcc agcggaaaga
    32341 gttgggtcaa atactggtag tcaaaggcat gatagagggc cagcaactgg aagagcaaga
    32401 ggtagttaaa gagaactacc actgctgtcg caatccaagc ttgctcgata aaaacctgcg
    32461 ccagtacgga aagtaagaga agacgaagac tgagagcaaa gaggtcgcca tttcgcagat
    32521 aagaacgcag atagagattt tgccaaatct tcccaggcac cttctgaaca gcctttaaaa
    32581 taaagtccag ataggcacga cgcttaacgc tgtttgaaat tcccttgacc tgcgtaaaga
    32641 gggcaaagaa acgaagcaag acttgcttac gcttgctttc ttgagaaata agatagtccc
    32701 agtccagtcc agtttcagtg aaaaatttgc tggccttttg acaaaagtgg aaatattttc
    32761 ctacccccaa taaaagcaca tagagcagaa aaactggcaa gccataaccc attgctaaaa
    32821 ataaaggcgc aaataacagc aagaaaaggg tctgtacaaa gagccaaaag actagggaaa
    32881 tgccagtttg acgcttgaga tggagcttga tttcctcttc tccaactaag agaaagagct
    32941 tgtctggagc ctccatatag gtggcaattc ctccccaaag taaaagtaaa acagacgtaa
    33001 ttcctacaaa caaaaggata ggccaatgat tttcaggaaa atgttgtaag agttgactgt
    33061 actggtaggc tagaaagccc aacaggacaa gcaggaacaa gacaaagtgg tcattgagca
    33121 catagcgcag ataaccaaga cactccttac gaaaggcctg ctttctcttt aaaaacaagt
    33181 ctttcatagg tcctcctctt ttgtcagagc caagtaaata tcattcaaac tagcctcagg
    33241 catatcaaag gcttcgcgta gttgcaggag attgcctttg gcacgcacct ctcccttgtg
    33301 aagaatgaca aaggcatcac acatcttctc cgccgaatcc agcacgtggg tactcatgag
    33361 aatagacttg cccttttgct tctccacttc caaaagctga atcaaatcag aaatagccag
    33421 cggatcaaga ccaaggaaag gctcatccac gatgaaaagg cttggatcca ccacaaaagc
    33481 acagataatc atgaccttct gcttcatccc ttttgaaaaa tgaacaggga accagtctaa
    33541 tttctggtcc agacggaaca tctttaacaa gggctctact cgttcgaaag ccactttttg
    33601 ctcaataccg taagccatag caaccgtttc gatatgctct ctgagggtca attcctcata
    33661 cagactaggc gtctcaggaa tgtagccaat ctgcttgcgg tagctagtcg catctccttg
    33721 cagagtcagg ccattgatat tgatggagcc actataaggt gccaacagac cgataatctc
    33781 attgatcgtc gttgatttcc cagcaccatt gagaccaatc aaaccgacca actgcccact
    33841 ttcaacagta aaggacacat ctttcaaaac aggaacatga acatagccac ctgtcaggtt
    33901 tttaatttct aacatatttt ctccaaatct ggtataatgt agctatatta tatcaaaatt
    33961 tagtacagta gaggtagatt ttatgtcaga ttgcattttt tgtaaaatca tcgcagggga
    34021 aattcctgct tcaaaagtat atgaagatga gcaggtcctt gcctttcttg atatctctca
    34081 agtaacacta ggacacacct tggtcgtgcc aaaagaacac tatcgcaatc ttttggagat
    34141 ggatgctacg agcgccagcc aactctttgc ccaagtacca aaagtagctc aaaaagtcat
    34201 gaaagtcact aaggctgctg gtatgaatat catttccaac tgtgaagaag tcgctggtca
    34261 aacagttttt catactcacg ttcaccttgt gcctcgctac agtgctgacg atgacctcaa
    34321 gattgatttt atcgcccacg aaccagactt tgacaaactt gctcaagtcg ctgaaaccat
    34381 caaaaacgct taaggagttg ctatgaaatt atccaaccta ctgctatttg caggagctgc
    34441 agccggaagt tatctggtta caaaaaatcg ccaaaccatc acagatgaag tcttgaatac
    34501 cactgaccgc gttcaagcta tcaaggacga tgtggatatt atccaaaaca gcctgcaaat
    34561 cattaaccag caaaaagaac ttataaagga ataccaagaa gacttgactt acaagtttaa
    34621 ggtcttggaa aaggatatcc agactagact agctgtgata aaagaaatgc agggaactga
    34681 agataagtaa aaagagcccc ccggctcttt tttaaaacgt aaacaagtta tagtgaattg
    34741 aatttagaat agcacaccat agtttctaaa gcattgttag agattacttt aaaatcctct
    34801 tatcaatttg ttcatattct atttcaatct attatatata tcccatcaac tatgcatcat
    34861 aatttaggta actcatactc aataaaaatc aaaaagcaaa ctagaaagct aggcacagac
    34921 tgctcaaaac accgttttaa ggttgtggat agaactgacg aagtcagctc aaaacactgt
    34981 tttgaggttg tggatagaac tgacgaagtc agtaaccata cccatggcaa gccgacgctg
    35041 acgtggtttg aagaaatctt tgaagagtat cattcaccat ttcacaacta aatcgaagac
    35101 tttctgccgt aatgaaaaca cctgaaacag cttggtttca gctgtccgga aactttaaga
    35161 cttaggttca aagtttaggt atggaacttc gaaggaggtc gctaccgtcc gtcattactt
    35221 aggaaagtct taaaaaatct ataaagtaaa aagagcccga cagctctttt tgtttatact
    35281 caatgaaaat caaaaagcaa actaggaaac tagccacggg ctgctcaaaa cactgttttg
    35341 aggttgtaga tacgactcac atatatacgg caagccgacg ctgacgtggt ttgaagagat
    35401 tttcaaagag tattattctc catcaaaggc atctttaata tgatcaaaga agcctttttt
    35461 ctttggattt actttcaagt caccagcagc cgcgaattct ttcaaggcta ctttttggcg
    35521 gtcgttcaag cctgtcggtg ttacgacatt aacagtaacg tattggtcac caactgcacc
    35581 gccacgaagg ctcggtgccc ccttactacg taggcggaat ttcttaccag tctgagttcc
    35641 ctctggaata accaattcaa catcaccgtg aacagttgga atatctactg tatcaccaag
    35701 agccgcttgg acaaagttga ggttgagatt gtagaagata gtcgttcctt cacgttcaaa
    35761 cttgtcgcta gcttccacag aaactactac atacaagtca ccataaggtc caccgttaaa
    35821 gcctgcttca ccttgaccag cgaggcgaat ttgttgacct gtttccacac cagcagggat
    35881 tttcacatgt acgctatgag cttgtttctc atgacctgtt ccatgacagg ttgtacatgg
    35941 atatttgatt tcttttcctt ggccgtgaca gacatcacag gttacttggc gacgcatcat
    36001 accaagagga gtctgcgtat cgacgttaat gacaccagcg ccatgacagc gtccacaagt
    36061 gactggactt gtccctggct tagcaccaga gccattacat gtacgacagc cagcttcacg
    36121 atgatactta acttccttct cagttccgaa gatagcttct tcaaaggtca aattgacacg
    36181 atactggaga tcatctcctt ggcgaggagc gtttggattg cgcgaagaac cgcctccgcc
    36241 gaagaaactt gagaaaatat cctcaaaacc accgaagcca cctgccccat tgaaaccgcc
    36301 gaaaccacca tctccaccaa aaccaccatt ggcgcctgca gcaccatact ggtcataggc
    36361 agcacgtttt tggtcgtcac tcaaagtctc ataggcttct tgaacttcct tgtacttgtc
    36421 ctcagcacca ggctccttgt tgatatctgg gtgatatttt ttggaaagct tacgataagc
    36481 ctttttgatt tcgtctgccg aagcgttttt ggataccccc agacgatcat aaaattcagt
    36541 attgttcata attcaagata caaagggcgt caaacggaca cagccatcta ggagttttga
    36601 cgacggacgc ttatgtccta gaaaaaacta tctttttggc acagtccgta gcccgtgttc
    36661 agttacgaac acccttattc ctttctattg atgttctgaa tcgaaccacc atttaggtcg
    36721 gattcaattc ctttctttta tatttagtaa gaaattctga aacccgtgtt cagttacgaa
    36781 cactctttgt tcctttctat tgatgagaca aaacccaaac aaatgagatt ccgtcccttt
    36841 ttatcgaaaa acagaggctg ggttgcaacc tctgcaaata agtaaattaa ctttattttg
    36901 attttaaata tcctataagt acagaaatat ttatatattt ttgtctcttc ttagtatttg
    36961 cctatataat tccaattgat cataagaaat acaaatactg tccaaatcat accaattatg
    37021 aacggtatta caaatttata tgtttgtatt tctaatgata tcatagctga taccatcgcc
    37081 gatacaaatg cagacaacaa tactgaagta acaaaattcc ttaccgcttc atcatttttg
    37141 acacaactat catgctcagc taatataatt tctctatata tagtcaaaca ttctactgga
    37201 atattatcta taaaaccttt tgctgcttca tagttgtcag cttgattaca aaaatcatta
    37261 taaacttttc gtgtattttt tagatactca tcatccaata cactcatctt atttttccgt
    37321 aaactctccg tctacgacgt catcgcctgc gtttcctgtt gcttgtgcgc cttctgctcc
    37381 ttcttgagct tgttgcgctg ctgcggcttg ttcgtagagt ttcacagcaa ggccttgagc
    37441 tttttcgttc aacgcttcaa gtttcgcttt catgtcgtcc aagttgttgt cttcttgagc
    37501 tttcttaagg tcatcaaggg cagcttgggc agcgtcacgt tctgcgtcga agcctttacc
    37561 ttcagtttcc ttgattgtct tttcagtcgc aaagattgct tggtctactt cattacgaag
    37621 gtctacttct tctttacgtt tcttatcggc ttcagcgttt gcttctgcat ctttcatcat
    37681 gcggtcgatt tcttcgtcag tcaaacctga gttcgattgg atgacaatag tttgttcttt
    37741 ttgagttcca aggtctttgg ccttaacaga cacgataccg ttcttgtcga tgtcaaatgt
    37801 tacttcgatt tgaggaattc cacgaggtgc agctgggata tcagtcaatt ggaagcgtcc
    37861 aagagtcttg ttatctgctg ccattgggcg ttcaccttga agaacgtgga tatcaacggc
    37921 tggttggttg tctgctgctg ttgagaagac ttgtgattta gatgttggga ttgtagtgtt
    37981 gcgatcgata agttttgtaa atactccacc cattgtttcg ataccaagtg acaatggcgt
    38041 tacatcaaga aggacaacgt ccttgacatc accagtaatc acaccacctt ggatagccgc
    38101 acccatagca actacttcat cagggtttac tgatttgttt ggttctttac cagtttcagc
    38161 tttaacagct tcaacaacgg cagggatacg agttgaacca ccaacaagga taacttcgtc
    38221 gatttctgac aagctcaaac ctgcatctga aagggcttga cgaactggaa cttttgtacg
    38281 ttcaacaagg tcacgagtca aatcatcaaa tttcgcacga gtcaaagtca tttccaagtg
    38341 aagaggtcca gcctcacctg cagtgataaa tggcaagctg atttgtgttg aagttacacc
    38401 agaaaggtct ttcttcgctt tttcagccgc atctttcaaa cgttgcattg ccatcttgtc
    38461 agtagacaag tcgataccgt tttctttctt gaattctgct accaagtggt caatgatttt
    38521 ttggtcaaag tcgtcaccac caagtttgtt gtcccctgca gttgacaata cgtcgaagac
    38581 accgtcaccc aattcaagga tagagacgtc gaatgtacca ccaccaaggt caaataccaa
    38641 gattttttct tctttgtcag tcttgtccaa accataagca agagctgctg cagttggttc
    38701 gttaacaata cgttctactt caagaccagc aattttacca gcgtcttttg ttgcttgacg
    38761 ttgagcgtcg ttgaagtaag ccggaactgt gataacagct ttggttactt tctcaccaag
    38821 gtagtcttca gcgtagcctt tcaagtattg aaggatcata gctgagattt cttgtggagt
    38881 gtattctttt ccatttgcag aaactttttc agaagttccc atcttagatt tgatagagat
    38941 aactgtatct gggtttgtaa ctgcttgacg ttttgcagca tcaccaacga tgatttctcc
    39001 gtttttgaat gagactacag atggagttgt gcggtttcct tctgggtttg cgatgatttt
    39061 gctttcagtt ccttcaagaa ctgcaactgc tgagtttgtt gtacctaagt caataccgat
    39121 aattttagac atgtgttttt ctccttgtta gttaattttc tatttttctt tttgatactc
    39181 ttcttaagtg gcgacgccgt cagagcttga cttcgtcaat ctctttgact aaactttgag
    39241 cctaaggtct caaaatttgc gcaaatagcg ccacggcgaa gagtatcgtg tttggttcgc
    39301 ttcgctcact attacaaagc caaacatatt ttatctttct tcatagttta ttgtcgtttc
    39361 ggacaagttt tctattatgt aaattgcgac acgaggagtc gaaatcgatt ttatttcgac
    39421 gacgagttag taaggaagct aggcaaacgc catagcgatt gccgttttct gacgagttgc
    39481 tttagctacc gtcagaattg ccgaatcgaa cacgccctaa gctctgagta aaaaagatag
    39541 attttcttgt gttcatcgaa cacttcgtca atctcctatt tttactgcga gctttttacg
    39601 ggctttatat cttagttata caccactacc atcgctgggc gtaggatgcg gtcatggagt
    39661 ttgtagcctt tttgaaagac ttgggcgatg gtatctactg ggtgttcatc gtctgctggg
    39721 agagtttgga tggccatatg gtagttatgg tcaaattcgc catctgctgc gatttcttca
    39781 attccttctt ctttcaaagc gtgaatcaag ctttcttgca ccatccccaa gcccttcttc
    39841 acatcatctg tcaaaccttc aactgcaagt gcacgctcaa ggttgtcaag agatggtaag
    39901 attgcttttg ccaagtcctg gctacgataa cgttgcaagt tttgacgttc ttcattggca
    39961 cggcgttgga tattttgcat ttctgcatga gcgcgaagat atttgttttc gaactcatct
    40021 gcacgttcat ttgccaagtc caactcagac ttttcaggag ttgtttcttc agctgtttcc
    40081 acaacttcct cttcttgaac ttcttctact tcttcatttt ttatatcttg ggccatgttc
    40141 gcctccttta atgatttcaa tcttaatgta cttcgtaatg attactgctg aggtagcggt
    40201 aaaaatctgt caacttcatg gtcaaaacac ggttgaccac attgacttga ttgattagct
    40261 gttggtaatc cagattaact ggaccgataa tggctagaat tccaactccc cgataaggaa
    40321 tgaggaactt actactaatc accgctaggt cagctaaaca ggactcttga ccgtctgcaa
    40381 cacgaacatt ttgcatctgt tcctcacgca acccctcacg aatctccaag gccacctttt
    40441 gcggttggtc aaagaactga taggctgcta gattggcaaa attcaagaga tggaccttgc
    40501 ccgccatcac aatgttttcg ttgaacattt ccttaaagat gtgttcaaag agatcgatga
    40561 cattatccgt tgttgtaaag taacgctgga taatctgcgg aatctccgtc cgaatcttgt
    40621 agtgaatatc taaaacggtg tgaccgagga aacgttcctg aatgatgctc ttcagtttca
    40681 gcaaatcctc ctgcaagaag ttccttggaa tcagaaactg actagtaacc gttcgcgact
    40741 cgtctagggt aaataccgcc aaggctgtat gttgccccaa aacaacgata tcaaaggctg
    40801 tcaaccgttg cctgctcggc tcaacatcca gtgccactac cgtacagcca ctcaggtctg
    40861 ttagtaagtt agcagcctct tgcagaatat cctccaattt gaagaattcc tgatcaaagg
    40921 ctttgacaat ctcatatacc tcattttcag ccagccggtc aaaatccagt gagtgtttca
    40981 catagtactg aaaaccagca acacttggca tccgaccgct tgaagtatga gccttctcaa
    41041 gcaacccttg tttttctagt tccgccatgt cattacgaat ggttgcactg ctagagttaa
    41101 tagactcttg caaggctttt gatccgacag gttcgtgcgt tttggtaaag atgtcaataa
    41161 tcagatttaa aatatcctgc tgacgctctg taaccatctc atcactcctc tctgtctgat
    41221 caattagcac tcaatgcatc caagtgctaa cttatgattc tattatacac ccttagaaga
    41281 gaatgtcaag acaaaaactc aaaaaattag cactctttta tcaagagtgc taaaaatcaa
    41341 tctgaagacc tagaaccttg ctattcatct actcggtatt tcctttttag tctgaaataa
    41401 ccatgaatta gataagaaat cagaaaagca tataaaacaa aaataagaaa gaaagacata
    41461 gaaagatcct ctctaaacaa actcgtacaa acaacaagac cacctaagaa agtagctgta
    41521 caggaacgaa gtctagatcg cataacttcc catttgagcc ccttactcat atagacttga
    41581 tcctctggaa gtaaatcaga agacgattta cgaagaatcg aacaagaacc tgctcctatc
    41641 aattcccaac ctctatctct aaaatcttgc agttcatgct tatatttttt aagaaatcta
    41701 gaatcataga tacggtagat gacatcgtct ggttgacatt ggtcaaaata gaacaaacca
    41761 aaacgactag ttctatacct ccaacctttc aaatgcatct catgtaaata ttcttcttcc
    41821 ttgtccaaat caacaatggt gaaaatccga aattctactc tgctattcat tgtcttaccc
    41881 caaaattaga aaacatgcct ggcgttattt attagataat tctttccact tttgactcaa
    41941 tctccaaaaa atataagaaa tctgaatcgt aaaaactatc aataaaaccc aatctattat
    42001 gaaaatcaaa aacactttcc aactgaaaga actacctcca gtgacaaact ttgagaaaaa
    42061 cggtagtaga gctaaaaaga gaagtaaaat aggaagcatc cgcattgtta aaatccgttt
    42121 ggcataaaaa aatctttatt taaacgaaaa tattatagca aaatttacgc cagtttttga
    42181 acggctgatg tagatatttt atactttcaa aatgtttaaa tgtgattatt tatttttgaa
    42241 aaatagctca ccagcccgac tgaaagtgct tatagaatga taataagtcg cctgccgaaa
    42301 acagcggaaa atagcggtgt tatgcggaga taatctgacg cgatgcgaaa gtatattgca
    42361 tacttatttt caacaattta gcagagtatt tttataagtg tgatataata gaagtataat
    42421 ttgttctgat agtttatttt atggagaagt agatttttag aatgcggagg gttcaatatg
    42481 gttgagttta taaagtctaa gaaagaaatg agtgaggagg atattaaagc aaatttcatc
    42541 actcctgcta ttgtatccaa aggatggaaa aatggtgagc atatcgctta cgaagaatac
    42601 ttcactgatg gtcgaattga ggttagagga gataaggctc gtcgtaaaga aggaaaaaaa
    42661 tcagactatt cactgtatta ccaatttgga actcgaattg caattgttga ggcaaaggat
    42721 aataaacaca gcgttcgagc aggattacaa caagctattg aatatggaga gattttagat
    42781 gttccatttg tttattcttc aaatggtgat ggctttattg aacacgaccg tatcacgaga
    42841 gaagaacgtg agctggagtt agacgaattc cctactcgtg aagaattatt ttctcgtatg
    42901 acgaaggaaa aaggattgac gtacgaaatt acagaagcta tctcaactcc atactataca
    42961 gacgccttct caatgaaaac gccacgctat tatcagcaaa tagctatcaa ccgtactatt
    43021 gaaacagttg ccagaggaca aaaacgagta atgtttgtga tggcaacagg aacggggaaa
    43081 acgttcatgg cttttcaaat tattcatcgc cttcgaaaag ctggtttggc taaacgagtt
    43141 ttattcttag cggatagaaa catcttagta gaccaaacga tggctgaaga ctttaagcca
    43201 ttcgaaaagg taatgacgaa aattacacca aaacttttga ctgctcctga aaaattaaat
    43261 tcttttgaaa tttatctagg gctttatcag caactaactg gtgaagatgg aactgaaaca
    43321 cattatcaaa aatttgacaa agacttcttt gatttaatcg taattgatga agcgcaccgt
    43381 ggttcagcta aggaaaacag taactggcgt aagataattg attatttcag ttctgcgaca
    43441 cagattggga tgaccgctac tcctaaagaa accaagaatg cttccaatac ggaatacttt
    43501 ggtgagccaa tctatactta tagtttaaaa cagggaatcg aggatggttt tttggctcca
    43561 tatcgtgtta tgagggttaa tttagatgtg gatgtggatg gttatcgtcc agaaactgga
    43621 aaagttgata ctaacggaca attaatagaa gataggtact acggcaggaa agattttgat
    43681 aaaaccattg tcattgatga tagaacgcaa agagttgcca agtttgtttc tgattatatg
    43741 aagcaaagca atgcacgatt tgataaaaca attgtttttt gtgttgatat tgaccatgcc
    43801 gagcgaatgc gtgctgcact tgtaaaagag aatctagact tagtccaaga agactatcgt
    43861 tatgtcatgc aagtaactgg tgacaacgct gaaggaaaag ctcaactgga taactttatg
    43921 gatgtcaatt ctaattttcc cgctattgta acaacgtcta aattattaac gacaggagtt
    43981 aatgctaaaa catgtcgttt gattgtttta gactctaata tccaatccat gactgaattt
    44041 aaacaaatta ttggtcgtgg cacacgtctt tatcctcaaa aggggaaaga attttttacg
    44101 attattgatt ttcgaaatgt taccaatttg tttgctgacc ctgattttga tggtgatcct
    44161 gtgaaggtgc tggaaacagg tgcgaaaaca gtcagtggtt ctacgcccgg tttcgtagat
    44221 gaggaaggtg acccagtaga aaaatatatc gttacagaca agcaggttac cattcttaat
    44281 tctactgttc aagtattaga tgaaaacggg aaactgatta ccgaaagcct gaccgactac
    44341 actcggaaga atatcttagg tagctacgcc actttgaacg attttatcac agtttggcat
    44401 acggcggata aaaagaagct tatcttagac gaactttata aaaaaggagt ttatctagat
    44461 gctattcgag agtcggaggg aatatcagaa caagaaatcg atgattttga tttactccta
    44521 aaacttgcct atggtcaaaa agaattaacc aaaacggaac gtatcaataa actcaaacaa
    44581 agcggatatt tatataaata tagtgaggaa gcgcgtgctg ttttggaaat tttactgaac
    44641 aaatacatgg ataaaggtat tggagaactc gaaagcattg aaacattaaa acttccagaa
    44701 tttcagatat atggtggaac cttcaaaatc atcaatactt attttggaga taaaaaacga
    44761 tatttacaag caattaaaga attggagcaa gagctattta cagtagctta atgaaaggaa
    44821 agtatgtcaa ttacatcatt tgtaaaaaga attcaagata tcactcgaaa cgatgctggt
    44881 gttaatggtg atgctcaacg tattgagcaa atgtcttggt tattattctt aaaaatttat
    44941 gatagccgtg aaatggtttg ggaattagaa gaagacgagt atgagtcaat tatcccagag
    45001 gaattaaaat ggcgaaattg ggctcatgct caaaatgggg aacgggtatt gacaggcgat
    45061 gaattacttg attttgtcaa taacaagtta ttcaaagagt tgaaagagct tgaaataact
    45121 tcaaatatgc ctattcgaaa aacgattgtt aaatcagctt ttgaagatgc gaacaactat
    45181 atgaaaaatg gcgtcttgtt acgccaagtc atcaatgtta ttgatgaagt tgatttcaat
    45241 agccctgaag atcgtcattc gtttaatgat atttacgaaa aaattcttaa agatattcaa
    45301 aatgctggga actcaggaga attttatacg ccacgtgcag cgactgattt tattgccgaa
    45361 gttcttgacc caaaacttgg agaatcaatg gcagaccttg cttgcggaac aggaggcttc
    45421 ttgacttcga ctctgaaccg tttaagtagt caacgtaaaa ctagtgaaga taccaaaaaa
    45481 tataatacag ctgtttttgg tattgaaaag aaagcatttc ctcatctttt agcagttaca
    45541 aatctgtttc ttcacgaaat tgatgaccct aaaattgttc atggaaatac tttggagaaa
    45601 aatgttcgtg aatatacgga tgatgaaaaa tttgacatta ttatgatgaa tccacctttt
    45661 ggagggtcag aattagaaac aataaaaaat aactttccag cagaattacg gagttctgaa
    45721 acagctgatt tatttatggc tgtcattatg tatcgtttga aagaaaatgg tcgtgttgga
    45781 gttattttac ctgatggttt tctatttggt gaaggtgtaa aaactcgctt gaaacaaaaa
    45841 ctggtagatg agttcaactt gcatacgatt attaggttgc ctcatagtgt ctttgcaccg
    45901 tatacaggaa tccatacgaa cattcttttc tttgataaaa caaagaaaac agaagaaact
    45961 tggttttatc gtttagatat gccagatggt tataaaaatt tctcgaaaac taagccgatg
    46021 aagtcagaac acttcaatcc tgttcgtgac tggtgggaaa atcgtgaaga gattctggaa
    46081 ggtaagttct acaaatctaa atcatttaca cctagtgaat tggctgagtt gaattataat
    46141 ttagaccagt gtggctttcc aaaagaggaa gaggaaatct taaatccctt tgagttgatt
    46201 cagaattatc aagcggaaag agcaacttta aatcataaga ttgataatgt attagctgat
    46261 attttgcagt tgttggagga caaataatga caccagaaca acttaaagca agtattctcc
    46321 aaagagcgat ggaagggaaa ttagtgccgc aaaatcccaa tgacgaacct gcaagtgaat
    46381 tattaaagag aattaaagct gaaaaagaaa aacttatcag tgaaggaaaa atcaaacgag
    46441 ataaaaagga aactgagata tttcgtggtg atgatg
//
LOCUS       DAVDWT010000014        46360 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVDWT010000014 DAVDWT010000000
VERSION     DAVDWT010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46360
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.014"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(11..1891)
                     /locus_tag="VUW50_000926"
     CDS             complement(11..1891)
                     /locus_tag="VUW50_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET7982845.1"
                     /translation="MVRFIGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRRVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(1885..2754)
                     /locus_tag="VUW50_000927"
     CDS             complement(1885..2754)
                     /locus_tag="VUW50_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET7982846.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAATGNALCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVDNFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(2838..5483)
                     /locus_tag="VUW50_000928"
     CDS             complement(2838..5483)
                     /locus_tag="VUW50_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HET7982847.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPELL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTLQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELIVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(5574..6854)
                     /locus_tag="VUW50_000929"
     CDS             complement(5574..6854)
                     /locus_tag="VUW50_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HET7982848.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLSYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIKNYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            7025..9109
                     /locus_tag="VUW50_000930"
     CDS             7025..9109
                     /locus_tag="VUW50_000930"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HET7982849.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSCFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDRDK
                     ASFQPHYLKIFSLRYQIETQLTPTCYGASIRLEQKQGKTLSLYLHVADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKASAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLKTASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIANCAKKLKNIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKENKWLDIHTEQNHSHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPNH"
     gene            complement(9149..10828)
                     /locus_tag="VUW50_000931"
     CDS             complement(9149..10828)
                     /locus_tag="VUW50_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET7982850.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGACLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDAALPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGSGHTVGYKRLLRGAVVEAQKIRVVITESQVLPLLTKISLYKT
                     PRLSQTVAVQGLAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIYVLAFQAGETEKSLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            11583..12812
                     /gene="arcA"
                     /locus_tag="VUW50_000932"
     CDS             11583..12812
                     /gene="arcA"
                     /locus_tag="VUW50_000932"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HET7982851.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            12870..13886
                     /gene="argF"
                     /locus_tag="VUW50_000933"
     CDS             12870..13886
                     /gene="argF"
                     /locus_tag="VUW50_000933"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET7982852.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            14051..14998
                     /gene="arcC"
                     /locus_tag="VUW50_000934"
     CDS             14051..14998
                     /gene="arcC"
                     /locus_tag="VUW50_000934"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET7982853.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIILTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            15209..16720
                     /locus_tag="VUW50_000935"
     CDS             15209..16720
                     /locus_tag="VUW50_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HET7982854.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            16742..18073
                     /locus_tag="VUW50_000936"
     CDS             16742..18073
                     /locus_tag="VUW50_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET7982855.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     LLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            18168..18295
                     /locus_tag="VUW50_000937"
                     /pseudo
     CDS             18168..18295
                     /locus_tag="VUW50_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
     gene            complement(18460..18751)
                     /locus_tag="VUW50_000938"
                     /pseudo
     CDS             complement(18460..18751)
                     /locus_tag="VUW50_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18739..18990)
                     /locus_tag="VUW50_000939"
     CDS             complement(18739..18990)
                     /locus_tag="VUW50_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HET7982856.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQLVQDIE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            19194..19271
                     /locus_tag="VUW50_000940"
                     /pseudo
     CDS             19194..19271
                     /locus_tag="VUW50_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(19389..20273)
                     /locus_tag="VUW50_000941"
     CDS             complement(19389..20273)
                     /locus_tag="VUW50_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET7982857.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(20418..21569)
                     /locus_tag="VUW50_000942"
     CDS             complement(20418..21569)
                     /locus_tag="VUW50_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET7982858.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVANGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIVEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(21715..23481)
                     /locus_tag="VUW50_000943"
     CDS             complement(21715..23481)
                     /locus_tag="VUW50_000943"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HET7982859.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTAIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(23538..26654)
                     /locus_tag="VUW50_000944"
     CDS             complement(23538..26654)
                     /locus_tag="VUW50_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET7982860.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            complement(26664..28958)
                     /locus_tag="VUW50_000945"
     CDS             complement(26664..28958)
                     /locus_tag="VUW50_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET7982861.1"
                     /translation="MKLWYKKAAANWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPRTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQDREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            complement(29017..29817)
                     /locus_tag="VUW50_000946"
     CDS             complement(29017..29817)
                     /locus_tag="VUW50_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET7982862.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            complement(29814..30587)
                     /locus_tag="VUW50_000947"
     CDS             complement(29814..30587)
                     /locus_tag="VUW50_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET7982863.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            complement(30612..31082)
                     /locus_tag="VUW50_000948"
     CDS             complement(30612..31082)
                     /locus_tag="VUW50_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET7982864.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKITGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            complement(31073..31504)
                     /locus_tag="VUW50_000949"
     CDS             complement(31073..31504)
                     /locus_tag="VUW50_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7982865.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            complement(31497..31937)
                     /locus_tag="VUW50_000950"
     CDS             complement(31497..31937)
                     /locus_tag="VUW50_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HET7982866.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSVSCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            complement(31949..32587)
                     /locus_tag="VUW50_000951"
     CDS             complement(31949..32587)
                     /locus_tag="VUW50_000951"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HET7982867.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            complement(32702..34105)
                     /locus_tag="VUW50_000952"
     CDS             complement(32702..34105)
                     /locus_tag="VUW50_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HET7982868.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDVDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGEITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            34281..35054
                     /locus_tag="VUW50_000953"
     CDS             34281..35054
                     /locus_tag="VUW50_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET7982869.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            complement(36223..37728)
                     /gene="adcA"
                     /locus_tag="VUW50_000954"
     CDS             complement(36223..37728)
                     /gene="adcA"
                     /locus_tag="VUW50_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HET7982870.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQSLDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(37738..38544)
                     /locus_tag="VUW50_000955"
     CDS             complement(37738..38544)
                     /locus_tag="VUW50_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET7982871.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            complement(38537..39241)
                     /locus_tag="VUW50_000956"
     CDS             complement(38537..39241)
                     /locus_tag="VUW50_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET7982872.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            complement(39241..39681)
                     /gene="adcR"
                     /locus_tag="VUW50_000957"
     CDS             complement(39241..39681)
                     /gene="adcR"
                     /locus_tag="VUW50_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HET7982873.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            complement(39836..41104)
                     /gene="dltD"
                     /locus_tag="VUW50_000958"
     CDS             complement(39836..41104)
                     /gene="dltD"
                     /locus_tag="VUW50_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HET7982874.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NHTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            complement(41097..41336)
                     /gene="dltC"
                     /locus_tag="VUW50_000959"
     CDS             complement(41097..41336)
                     /gene="dltC"
                     /locus_tag="VUW50_000959"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HET7982875.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            complement(41350..42594)
                     /gene="dltB"
                     /locus_tag="VUW50_000960"
     CDS             complement(41350..42594)
                     /gene="dltB"
                     /locus_tag="VUW50_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HET7982876.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLVMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            complement(42591..44141)
                     /gene="dltA"
                     /locus_tag="VUW50_000961"
     CDS             complement(42591..44141)
                     /gene="dltA"
                     /locus_tag="VUW50_000961"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HET7982877.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            complement(44162..44293)
                     /locus_tag="VUW50_000962"
     CDS             complement(44162..44293)
                     /locus_tag="VUW50_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HET7982878.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            complement(44614..45792)
                     /locus_tag="VUW50_000963"
     CDS             complement(44614..45792)
                     /locus_tag="VUW50_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7982879.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTCINLVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLLQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
BASE COUNT        13841 a        10113 c         8368 g        14038 t
ORIGIN      
        1 tcgaagagta ttaagtcgta taaatcgtcg acgctgtcgc aatggtatgc cactggttgg
       61 ctgtagttgc tgcgaaatcc ttgtctgcgt agaagtcagt aaatggtagg atttcaactt
      121 ccagctcgtc gatgcggtca agctgaccag ccagataaac ctcgatacgg ctttctgctc
      181 gtttgatgcg ttgcaagagt ccgcccatac ggatgtcaac tgtatccaaa ccaaagacct
      241 tgttttcttt cagccattgg tgactaaaga gggcatggaa gtcttcaatt tggcttctaa
      301 gttctggtaa ttcttgtctg gcgatttgtt gtaaactttc tttatcatcc gcttggtagg
      361 cctgacgaat gcgtcgtccc acatctactt tgctacttaa aatagcattc aactgggcct
      421 gagtttcaaa gagataggca tagtttccag ctttttcttt aatgttagca agcgtctcag
      481 cagcctgagc gaagtgcggt ttgtcctgtt caggtgtcat gtgttgatca agaatcggac
      541 aaagaatatc ctgataaaaa acatagcggt tgggattgat accgctgaga ttgcctggta
      601 ggtctggtaa gaggttggca aggtcaatct gcataaaatc ctcaaccgtt agaccagtat
      661 tggtcttgaa gtgcgcagac aaaccatcta ggtcattgcg atagctgagt tctgcccaga
      721 tttgcaagct tggtaggata gagaactggg cagtttcacc accattgtct ccccaacccg
      781 ttacgatgac ttctttaatc tgattggcac ggcaggcttt attagcctcg atagccacta
      841 gacggctaaa atggttgtga ggtgtaaagc caatccactt ccaagctccc cctgcaaatg
      901 caaggtcatg gctaatcttg tgatgattgc ggaaattacg gttgtatttt tcctcgctat
      961 cctgataata atcccagtaa accagagtca cacggtcttt gagacggtct aggtagacac
     1021 gagtttcctc tggaatttcc acatcacgat cgtactggcc atccgctgac atgagtttga
     1081 agaacatatc actccacatc tggcagtgga aaccatattt gtcagcaata tccagcacgc
     1141 gctccaagtg ttggcacatg aggagactac gatccacaac accgttcaaa atcaggtagc
     1201 gtcccaaacc aaccaagtgg gcttcgtcca tcccgatatt gaccctgcga gtcttcagtt
     1261 tagacaacgt ggcaaacatg ccatcaatca ggtcataaac cttttcttcg ccaatgagaa
     1321 gaatgtcctc tacatcacgg agctcctgca cttccttgac accccatttg acaaaggccg
     1381 acaagtgggc caaggtctgg atgcatggta caaaggtcat gtcaaactgt tgagcatagg
     1441 cttcgatttc ctgcaactcc tctgctgaat aagctccacg gaaatagcca aagtaaggct
     1501 gcccttcaat ctggtaagtg tcttccatgt aaagctcaaa ggttgagtag cccatgagag
     1561 ccaagacctc aatcatctgc ttggcagaag ccacattcag caccgcattt cgagaacagt
     1621 caaccatgta agccaaatct tcgtaagctg cttgttcctc aatctctact ttatcagctt
     1681 ctgctagaac tgttaccaac aaggataagg cacgataaag ttggtgaggt ttgcggtagg
     1741 tcaattgata gtgaccttcc tcaccctcga tagagataga ggcttggtca gactgagtga
     1801 cagccacttc cacatctggt agagaaatgt gaccttttaa aacctcaata gcttgcgttt
     1861 gtttgggact aagtcctata aatcttacca ttgcttttcc tcctgtagcc agttgacaag
     1921 ggcaccgtag agattggcat ctgcgtgata ggtgcaggcc tggataactg gtgcgaccgt
     1981 gtattcttcg taggcatcga caaagttatc aacagccttc ttgacacctt ggataaaatc
     2041 tggattttga ctgatagagc cacccagact gatgacatct ggatcgatga gatactggat
     2101 attgagcaag ccttgcgcca gattgcggtt catgcgctca atggcttctt gacaaagagc
     2161 attaccagtt gcggcctctt ggtaaatctt gcgaccgtcc caatcagtat gaccagattt
     2221 ttcaatcacg tatcgtacca tattcccagt tgatgctagt tgcgaccagt tattaagttt
     2281 ttcagcaggg gcaagggttg tcatgtagcc aaattctcca cccagaccgt ggcgacctcg
     2341 atgaagtcta ccattgataa tcatggctcc gccaatccct gtcccaatca cgacacaggc
     2401 tgcattttca agctctggat gagctagtag ttcactgagt ccaacgcagt tggcatcatt
     2461 ttctaaatgg acaggtagct gataagagct aagcgcctca taccaagaaa agccatggat
     2521 gtagggcacc gcactgaagc catcaatcac acctgtctct tgattaaccg cacctggaac
     2581 gctcatagca atcccactgt aatcctgttc tgacaagcgt tgatctagcc acgctagtaa
     2641 atcctccaag ttttcaggcg ttgaaatact tgtcttatcc agtattttcc catcaggagt
     2701 cagactggca aacttaatcc cagtccctcc gatatcaatc gttgcaatgg tcattgtttt
     2761 caccataaaa aggggcgaat cagtagttag ttcttacctt ttcgcccctc ctttcttttg
     2821 atgtttactt tttgaaatta aatttcttct tttttgatga attctgtacg aatctcttgt
     2881 ggggccaata gtcctgaatg aactggatat ggtcgctcaa gtaagtcaag aatggtttgt
     2941 tgatgttctg atatgcgcac attttcttga ctcatattat agtaacggag gacatagcct
     3001 tcttcatttt ccgccacttt aaaggctgtt ggacaaactt gcggtatgct gagaacagaa
     3061 tggctcaaga ggctaccagt cgcaaccacg cttccttcct gtctagcaag ctgaaggctg
     3121 gtaaacggtg tctgcaaggc tttggcacga cgataggctg agaagcgttc ttgggcttgg
     3181 tggcattcaa tcgcaaattc gacttcaaac tcacgcaagc attgtgcttc tggcgttggg
     3241 aagtagcccc agtcacctag ctcacctgat gcacgcaaaa tggtcacggc aatggtgtta
     3301 tccccaagga tttcgtattc attcaatccc ttgttggata cagtcacccc tttttcatcg
     3361 tcatacagac tgacaaaagc ttgttggtgt tgaggatttt caggattttc ccatgaagca
     3421 gctggtttgt ttggtcgtgt caccacctca tagatacttt cagaatcatt gcttggacgc
     3481 gtgttatgag tcttgaccaa gagacggata cggtgatcct tggcagtgtt agtaaagcga
     3541 gtcttgaagc ggatttgtgg attgtcaaca aagacaatca actcagtttc cagaggaatg
     3601 tttgtcaatt cttctgaccg tccagcctca cgcttcataa actcgatgat accttgttgc
     3661 tcttcttcta gcttttcatc cgcactgaca ggcacggtca attcatgttt gagcaagatt
     3721 ttagcatagc aagctgtgtt ttccaagacc tcgtggccct taagctctgc aaagattggc
     3781 tctgttcctt ttggttggaa atagatatac tcgtttccga tgtccccacg gtcttcaaag
     3841 cggataaagt cttcataggc ttcgtgagtt gtcttgtcat agactgtgat gttgtcatcc
     3901 acactcaccg ttacgaatgg tgtatcaatc actccgtttt ggtaaatacc ctcacggtgt
     3961 tcttgttttc cttccagcaa ttggagggtt gtccaagaaa gcggcgctag gtgaactgga
     4021 atggtcacgc gcacttgacg agcaatacga gcttggcgga acttgtcttt tggtaaatca
     4081 tactcaaaat tagctccgag gtcttcgatt gtagcctcta caggacgacc atccaagtcc
     4141 tccacacggt aacttggcaa gataagagca gccatctttt tgtagccttc tgttgggtgt
     4201 aattccttga aatcacaagt cgccacatca atcactgtgc tgacagtatc gaccttatca
     4261 tgcaagcctg tgttaatgac agtaaagaga tagtcacttt gagccttatc cgtagcaatt
     4321 ttacccttcc actcgttgag caaattactt ttaacaaagt ttcctacttg gttgaccttg
     4381 gcaaaacgcg tttccatctc gcggtgaact tcgtccacgc tacagccaca gatactatca
     4441 tgtggcgcat tctgcaaaag tgttttccaa gcataggtca actggtcctt gtggttgtgt
     4501 ccaccagtga taatagtcaa gggttctacc acttgctcta ggaggttgct attttcttgg
     4561 aaggcttgtt ttaggtaaat gcgggatgaa gaagtgttgg caagtgtgta ccagccatct
     4621 gtttcctgac tggtcaactc acctgtaaca gttgataaga gttcaggaag cgcaccttct
     4681 acagcttgaa catattcatc aaaagaacta tgaacaaaga ttacatccgg gaagagttca
     4741 tttgccacac gaatggcttc gctcagattt ttctggacag gctggtggtc acagccgttc
     4801 atcatcaacc attggttggt cgaagcgtag gcacgcacat ctgacaattt ttgtttccag
     4861 aaggtcaagg cctcatcttt gtcaactggg atttcattcc cattactgta ccagttggca
     4921 aagagaatac ctaaaacacg actaccatcc acaccctgcc agtacatttc tgaaaactgg
     4981 gatgtaaact gctcatcttc aaggacttgg ttgtcaaatc caatcggctt cacaccacga
     5041 ccaaaggccg ccacgtgaat gcctgatttt tgaagaattt gaggcgcttg tcccatattt
     5101 ccaaaggtat ctggaaagta gccaatctgg gttgatttac cccatttggc agcttcttgt
     5161 tgaccaatca aggtattgcg gacattggct tcactggaga tcaagtagtc atcctgcaag
     5221 atgtaaaagg gaccaatttt aagtttgccc tcgtcaatgt agcgttggac cttgtcacga
     5281 ttttcagggc gaatttgtaa gtagtcgtca aggacaatag tttgtccatc caagtggaaa
     5341 ctcttgaact cagggtcatt ttcaaagaga tcaaagagat tgtcaaacaa ttccaccaac
     5401 tgcatacgat ggctttcaaa aggcaagtac cactcacggt cccagtgact atgtgagata
     5461 atatgtacaa caacattttc catgaagtaa aacctcattc taaatttaaa ttttaacgtt
     5521 ttaaatgtaa acttgtgatt ctgataagaa tcggttgagc taaagcgagc cccttagcga
     5581 atatccaagt aatccaagac caactcacag aacatcatgt tagcccagga gaaccattca
     5641 cgagagtaga gggtcggatc atctacatga aagctttcgt gcatgacacc tgtaccacca
     5701 tcgcaggcaa ccagctgatc cagcaagaat tttttctctg ccttatctct tgttgtcaat
     5761 ccttggatag aaagggcgat tggccagata tagcgataga aggtatgaga acttccgaga
     5821 ccgctagcgt attctccttg gtagaaatat ggattttcag agctcaaaat ggtacgacga
     5881 gtagcttgat acacttcatc atcgaccgaa cagtagccca gatagggcgc agctagtaga
     5941 cttggtacat ttggatcatc catgatgctg gcatttccta ggccatccac ttcaaaagcg
     6001 taaatctttt cgcccttgct gttggtggtg taagcgtagt ttttgattcc ttcttggatt
     6061 tcatcctgaa gacgcttggc atcagcaata acactctggc tatcagctag gtttaatgct
     6121 gcgaagattt cttgcacata acccaagact actacagcaa acatatttga cggaatcaag
     6181 taactatact ggcaacagtc atcactcgga cgaaaagctg accaagtcat acctgtcact
     6241 gcaaagtcag gtccaaagcc atcatttacc aaggtgtctt ccttacggtc cgtatctcgg
     6301 acaaaacgat aaggagagtt cttgtggtct tgttctaccg tccacagatg aagaatttcc
     6361 ttagtcgctg cgacaaaagt ctcatcaaac tgactagtct cgccagtttc tttccagagg
     6421 agataagaca actgcaaagg atagcaaagc gagtccacct catacttgcg ttcccaaatc
     6481 cagccattaa ggtcggtgtg gtcggtctcg tggtgcccct tccagttttc ctctatgttg
     6541 aaggaattgg cataaggatc cttgagtacc aaggtcatct gacgtttgac caaacctgca
     6601 atggtctgac gcaggagggc atctctttta gctacatgaa ggtagggtct gagttgggct
     6661 gtcgaatccc gaagccacat agcaggaata tccccagtca agacaaaagt tgaaccatct
     6721 tctaagattt caaccgtatt gtccaaggta tcggtgtagc aacgctcgaa aacatccacc
     6781 cactctggat ggtccttagc ccgctctgct acttcatcca gccattctct aacaatttct
     6841 ttcgaataaa tcatcgtgca attccctctt tcaaacaagt ttcattttca gtatatagct
     6901 tttgagacag aaaatacata cacaaatgat agtcattttt ctacaaaata gttatctttg
     6961 aaactccttt tctaaaggcc ctctttttct gatataatgt agaagaacag ctaaaggaaa
     7021 gactatgaaa ccactacttg aaaccatcga tacccgcttt ggtactgcta gcaagcatgc
     7081 cttctctcgg ggaaataccc tgccatacac aggcgtgcct tttggaatga attactttgt
     7141 gccccagacc agtgaccagg atggatcttg tttttttgat ccgcatctgc ctatctttca
     7201 ggggattcga ttaactcacc agcccagtcc ttggattggg gactactctt ggctccttct
     7261 gacacctgtc accagtcaac tgggtggaga cagcctcttt caccgccaat cttcctatga
     7321 tagggataag gcctctttcc aacctcatta tctaaagatt ttctccctgc gctatcagat
     7381 tgaaacccag ctcacaccga cttgctacgg tgcttctatt cgtttggagc aaaagcaagg
     7441 caaaaccctc tccctctatc ttcacgtagc agatgaactg accgtagagc aagtagataa
     7501 gcggactctt gccctgcgac aagaaggtaa aactgaaact aacaaaaatt cgctaacgat
     7561 gttcactgcc ctgcaaatga acacggatat tcttgctatc agccaagaag ctggagactg
     7621 gcgaattgac ttagcaagta gtcaaaccga gatgcaacta gccacttctt tcatctctcc
     7681 ttctcaagct ctaatcaatc tacctcaaga agattttgat agctgtaaag caagtgccca
     7741 agcggattgg gaaaatctcc tccatcgttt tgacattata gagacaggag aggctgaccg
     7801 aaccttcttt gaccactgtc tctacagact cttcctcttc ccacagactt tttatgaggt
     7861 tgatgagtca gggcaagcca tccacatgga tctggctact ggtactgtca agcctggtgt
     7921 cctctttagc aacaatggtt tctgggatac cttccgcacc accttccccc tctttgccct
     7981 tatcataccg gagcactatc aacgcttttt agaaggtttc ctcaatagct accgcgatac
     8041 tggtttcctt ccaaaatggc tggctccaga tgaacggggt atgatgccag gtacactttt
     8101 agacggtatt atcgcagata gcgcctgcaa ggacatggcc cccgacctag aaggagaact
     8161 cttccaagcc atgctcaaaa cagccagcaa agccgaccct ctcggcatca atggccgcca
     8221 cggactagcc caataccaag aactaggtta cctctctacc gaccaccacg aaagtgttag
     8281 ccatactcta gactatgcct atagtgattt ttgtatcgcc aactgtgcca aaaaactaaa
     8341 aaacatagaa atcgctgaaa cctacaaggc tgcttcacaa aattaccgcc agctatttga
     8401 cgctgagaca ggttacatgc gagcacgaga caatcaagga aactttcacc ctgacttctc
     8461 tccttatagt tgggggcgag actacgctga atgctctgcc attcaagcta ctttaggcgt
     8521 tctccacgac atccctggct taatccaact gatgggtgga aaagaaacct ttagcaacta
     8581 tcttttgaaa gcctgtcaag atgctcccct ctttgaaaca acaggctatg gttacgaaat
     8641 tcacgaaatg agcgagatgg ctactgctcc ttttggacaa atagccattt ccaaccagcc
     8701 tagtttccac attccttatc tcttccgcta cagcgattac cctgactaca ctgcccttct
     8761 tatcaagacg ctccgtcaga aagcttttca cccaagttgg gaagcctatc ctggagatga
     8821 agacaatggt agtctctctg cttggtacat ctggtcagct ctcggatttt atccaacctg
     8881 tccaggaaaa ccaagctatg atctcggaat tcctctcttt gaccatctac gagtctacct
     8941 agctaaagaa aataaatggc tagatattca tactgagcaa aaccacagcc attttaactt
     9001 tgtcaaagaa tgccgactgg acaaaacact cgtatcaact attcaacacc aagacctcct
     9061 aaaagctgaa caactaactt tcaccctcag ctggctacca aatcactaat aaccaaaaga
     9121 acctttgcat cgcgcaaagg ttcttctttt atgaaacttg gacttgaagt tgatccacaa
     9181 gctgaccatc caccgttagg ttcagataga aatccaaggt tttatctcct gcgaaataca
     9241 aggttggtag cgtcagactt ttttcagtct caccagcttg aaacgcaagg acataaatct
     9301 catcctgata ggcgacacca tggacacctg tccccggttg aatcgaaatc ttagcctcta
     9361 aagaggagct actttcacta cgttcaatcc taaaatgaag agtctctccc tttgccacag
     9421 ctaggctttt ttctgcaaat gctagcccct gaacagctac tgtttgtgat aacctcggag
     9481 ttttatagag tgaaatcttg gtcaacaaag gcaaaacctg tgattctgta atgactacac
     9541 gtatcttctg tgcttcaaca actgctcctc gtaagagacg tttgtaacca acagtatgac
     9601 ccgaaccaaa ctcctgccag acaccatcca cctctacttg cacatgaaaa gcagcgattc
     9661 gttgccctag cttcaaatct tctcttaact caattacatc aaaagtttta ggagaaccta
     9721 agtcgagttc taactggatg ggcaaggctg catcgtttgc ccaagagctg gtctcaaggc
     9781 cgtctgtcaa atggcgacaa gcaaagtctg cggaaagagc tggaccagat acctcagctc
     9841 ccagagccaa atcttcttta tagagctcat tgcgataggt cgcaaattca taaagtcgtt
     9901 caatatcctt tgcatcaaag agcccagctt gattcggcgg aatattaagc aagagtggag
     9961 ttcctcgccc tactgagtga aagtagattt cgaccaactc ctcgagagac ttaggatcct
    10021 gatcctcatg gtagaaccag cctggacgga tggaaacatc tgcctctccg attgaaaaaa
    10081 tcgtgcccga gggatccccg tgctgaagat agttcagctc tgcttctgtt cctagtttat
    10141 caggattcac cttttgccac agtggatcac ctgcataccc tcgttcattg ccaatccagc
    10201 ggatactggt gccttctgtt gaaaaaatca agcaagcgcc ctgcaggtca cgaatggttt
    10261 caaaccattt ttcaaattca taattaacct tttgcgcgcc ctctcctctg gcaccatcca
    10321 tccaaacctc agcgaactta ccagcattcc catagttagg atttgataag atttccttca
    10381 actgagccag ataataggca ttgtagtccg cttctcggtc cacatgatag aggggactat
    10441 gggcatccca cggtgacagg tagaccccca tatccatatc aaactctgtg gcagcttggg
    10501 atacttcaag gagcaagtcg ccctttcctc tcctccaagg actgacctta accgaataat
    10561 ctgtgtgagc tgtcggataa aggacaaagc catcgtggtg cttgaccacc aaaatcaact
    10621 ttttgaagcc cgtttccttg agcacacgaa cccactcacg cgcatccaac tgactcgggt
    10681 taaagcgctc aggatcctcc tgtccagtcc cccattcttg gtcataaaag gtattaggac
    10741 cgaagtggat aaaagctgct agttcatctc ccagataagc tagctgagtc tgacttggta
    10801 acggtccatg cggtttaatt ttcttcatcg ttatatatct ccttctttag caacttctct
    10861 tgttcacaag gttatccaca ggttgtggat aataaaccat gatctaaccg ccagctcttt
    10921 ccaactccaa ctggtaggct caggattttt ctcctcaata ttccctcttc aaacagctct
    10981 gtcatatggt tctgctaaca cctctcagaa tgctactctc cataattatt ctcaggcttc
    11041 tttcaggtca ttgtgtgaag accattcact ctccagactt ttccacagat tgtgaataag
    11101 tgattattct aaactatcac ctcgtcgact ataacaaaaa ataaaaagag ggtcaataag
    11161 ataattatct cttcctaaat actcagaaaa gattctataa tttgtacaat tgaacaagaa
    11221 atgcttctcc aaaatctatc ttcaaaagaa tgttgcatct ttttatcctt tcacacaaac
    11281 atttctcttt atacagatcc aagtgataac cagatgataa ttggacactt tttggtcatt
    11341 tgtatatttt ttgaaaaatt ttttaaaaaa attttatatc ctttttatag aagtttatgc
    11401 atttgtatct atgtattcta tcctatgtta tgtataataa cataaaattg tgctaaatat
    11461 cataatttat attattttcc gagaaattat agccattgac attacctagg aattgcgtta
    11521 taacataaat gtaagcggta cccaaaatgg tgccaagtca aaatttttaa ggaggaaaat
    11581 acatgtcttc acatccaatt caggtcttct cagaaattgg gaaactgaaa aaagttatgt
    11641 tgcaccgtcc aggcaaggag ttagaaaact tgttgccgga ctatcttgaa aggcttcttt
    11701 ttgatgatat tcctttcttg gaagatgctc aaaaagaaca tgatgcattt gcccaagctc
    11761 ttcgcgatga aggaattgag gttctctacc tagaacaact cgctgctgaa tcattgacct
    11821 ctccagaaat ccgcgatcaa tttatcgagg aatacttaga cgaagccaac atccgtgatc
    11881 gtcaaaccaa ggttgctatt cgtgaattgc ttcacggcat caaggacaac caagaattgg
    11941 ttgaaaaaac aatggctggg attcaaaaag ttgaattgcc agaaattcct gacgaagcta
    12001 aagatctaac tgacttagtt gaatcagatt atccatttgc aattgacccg atgccaaacc
    12061 tctatttcac tcgcgaccca tttgcaacaa ttggaaacgc cgtatcgctt aaccacatgt
    12121 ttgcagacac tcgtaaccgt gaaacactct acggtaagta tatcttcaaa taccacccaa
    12181 tctatggcgg aaaagtggat ttggtctaca accgtgaaga agatacgcgt atcgaaggtg
    12241 gagacgagct agttctttct aaagacgtcc ttgcagtagg tatctctcaa cgtacagacg
    12301 cagcttctat cgaaaaactt ttggtcaaca tcttcaagaa aaatgttggc ttcaagaaag
    12361 ttttggcctt tgaatttgct aacaaccgta aattcatgca cttggatact gtcttcacta
    12421 tggtagacta tgacaagttc actattcacc cagaaatcga aggcgacctt cacgtttact
    12481 cagttactta cgaaaacgaa aaacttaaaa tcgttgaaga gaaaggtgac ttagctgaac
    12541 ttcttgctca aaaccttggt gtagaaaaag ttcatttgat tcgttgcggt ggtggcaata
    12601 tcgtagcagc tgcgcgtgaa caatggaacg acggttctaa cactttgacc atcgcacctg
    12661 gtgtggtagt tgtttatgac cgcaataccg tgaccaataa gattttggaa gaatacgggc
    12721 ttcgcttgat taagattcgc ggaagtgaat tggttcgggg ccgtggtgga cctcgttgta
    12781 tgtctatgcc atttgaacgt gaagaagtgt aatcgctgtt cggcattcgt caatagaaaa
    12841 tgtaaaaaat agaaagagga aataataaaa tgacaaattc agtattccaa ggacgcagct
    12901 tcttagcaga aaaagacttt acccgtgcag agttagaata ccttattggt ctttcagctc
    12961 acttgaaaga tttgaaaaaa cgcaatattc aacaccacta ccttgctggc aagaatatcg
    13021 ctctcctatt tgaaaaaaca tctactcgta ctcgtgcagc ctttacaact gcggctatcg
    13081 accttggtgc tcacccagaa tacctcggag caaatgatat tcagttgggt aaaaaagaat
    13141 ctactgaaga tactgctaaa gtattgggac gtatgtttga cgggattgaa ttccgcggat
    13201 tcagccaacg tatggttgaa gaattggcag aattctcagg cgttccagta tggaacggtc
    13261 taactgacga atggcaccca actcaaatgc tcgctgacta cttgactgtt caagaaaact
    13321 tcggtcgctt ggaaggcttg acattggtat actgtggtga tggacgtaac aacgttgcca
    13381 acagcttgct cgtaacaggt gctatccttg gtgtcaatgt tcacatcttc tcaccaaaag
    13441 aactcttccc agaaaaagaa atcgttgaat tggcagaagg atttgctaaa gaaagtggcg
    13501 cacatgttct catcactgaa gatgctgatg aagcagttaa agatgcagac gttctttaca
    13561 cagacgtttg ggtatcaatg ggtgaagaag acaaattcgc agaacgtgta gctcttctta
    13621 aaccttacca agtcaatatg gacttagtta aaaaagcagg caatgaaaac ttgatcttcc
    13681 tacactgctt gccagcattc cacgatactc acactgttta tggtaaagac gttgctgaaa
    13741 aatttggtgt agaagaaatg gaagtaacag acgaagtctt ccgcagcaag tacgctcgcc
    13801 acttcgatca agcagaaaac cgtatgcaca ctatcaaagc tgttatggct gctacacttg
    13861 gtaaccttta tattcctaaa gtataatttt agataataaa ccgtctacca acagctatga
    13921 gggctgcgac taatagcttt agtccggtcc tcttttatgt aatggtaatc tattatttct
    13981 tataaaatat gtgaaaaatc attaaattga aatctaaacg cattctattg agtgtgataa
    14041 aggagaattt atggcaaatc gtaaaattgt agtagctttg ggaggaaatg cgattctttc
    14101 ttctgaccca tcagcaaagg ctcaacaaga agctttagtt gaaacagcta agcatcttgt
    14161 aaaattgatt aaaaatggag atgatctgat tatcactcac ggtaatggac ctcaagttgg
    14221 gaatctcttg ctccaacatt tggcatcaga ctctgaaaag aaccctgcct tcccactcga
    14281 ctcacttgtc gctatgacag aaggtagcat cggtttctgg ttgaaaaatg ctttgcaaaa
    14341 tgctctcttg gatgaaggca tcgaaaaaaa tgttgcctct gttgtaacgc aagttgtcgt
    14401 agataaaaat gatccagctt ttgttaactt gagtaaacca atcggtcctt tctattcaga
    14461 agaagaagca aaagcagaag ccgaaaaaag cggagcgact ttcaaggaag atgctggccg
    14521 tggctggcgt aaggtcgttg cctcaccaaa acctgttgac atcaaagaaa ttgaaaccat
    14581 ccgtactctt ttaaataatg gtcaagtcgt cgtagctgca ggtggtggcg gtattcccgt
    14641 cgtcaaagaa aacaatggac atttgactgg tgtcgaagcg gttattgata aagacttcgc
    14701 ttcccaacgt ttggcagaat tggttgatgc agacctcttc atcattttga caggtgtaga
    14761 ttatgtattt gttaactaca acaagccaaa ccaggaaaaa ttggaacatg tgaatgttgc
    14821 ccagctggaa gaatatatca aacaagatca gtttgcacca ggtagcatgc ttccaaaagt
    14881 agaagcagct atcgcttttg tcaatggtcg tccagaagga aaagcagtta ttacttccct
    14941 tgaaaatcta ggcgccttga ttgaatctga aagcggaaca attattgaaa aaggataagt
    15001 tgttttacta ataagatgta ttctatttct agtatcttta tatcaaatta gaaattattc
    15061 ttgaaaacat gtacaatatt tcaaaagata ttagtttttt agactttaat atggtaaaac
    15121 aaatataaat agaaagcgtt ttcttaaatg tttatttaag aaagtagttg gttttttaca
    15181 ctttgttaga catcaggagg aaaaacaaat gagtgaaaaa gctaaaaaag ggtttaagat
    15241 gccttcatct tacaccgtat tattgataat cattgctatt atggcagtac taacttggtt
    15301 tatccctgcg ggggccttta tagaaggtat ttacgagact cagcctcaaa atccacaagg
    15361 aatttgggat gtcctcatgg caccgattcg ggctatgcta ggtactcatc cagaggaagg
    15421 ttcgctcatt aaagaaacga gcgcagcgat tgatgtagcc ttcttcatcc ttatggttgg
    15481 tggtttcctt ggcattgtca acaaaactgg tgctcttgac gtagggattg cctctatcgt
    15541 gaagaagtat aagggccgcg aaaaaatgtt aattttggta ctgatgcctt tgtttgccct
    15601 cggtggtaca acttatggta tgggtgaaga aacgatggcc ttctatccac tccttgtgcc
    15661 agttatgatg gccgttggtt ttgatagcct gactggtgtt gcaattattt tgctcggttc
    15721 tcaaatcggc tgtttggcat ctactctgaa tccatttgcg acaggtattg cttcagcgac
    15781 tgcgggagtt ggtacagggg acggtatcgt acttcgtctg atcttctggg ttaccttgac
    15841 tgctcttagt acttggtttg tttaccgtta tgcggataag attcaaaaag atccgactaa
    15901 gtcactggtt tatagtactc gcaaagaaga tttgaaacac tttaacgtag aagaatcttc
    15961 atctgtagaa tctacactta gcagcaaaca aaaatcagtt ctctttttat ttgtgttgac
    16021 attcatcttg atggtattga gcttcattcc atggacagac cttggcgtta ccatttttga
    16081 tgactttaat gcttggttga ctggtcttcc agttattggt aatattgtcg gttcatctac
    16141 ttctgcacta ggtacttggt acttcccaga aggcgcaatg ctctttgcct ttatgggtat
    16201 cctgattggt gttatttatg gtcttaaaga agataagatt atctcttcct tcatgaatgg
    16261 tgctgctgac ttgctcagtg ttgccttgat cgtagcgatt gctcgtggta ttcaagttat
    16321 catgaacgac ggtatgatta ccgatacaat cctcaactgg ggtaaagaag gcttgagcgg
    16381 tctatcttca caagtcttta tcgttgtaac ttatatcttc tatctaccta tgtcattctt
    16441 gatcccatct tcatctggtc ttgccagcgc aactatgggt atcatggctc cacttggaga
    16501 atttgtaaat gtccgtccta gcttgattat cactgcttac caatctgctt caggtgtctt
    16561 gaacttgatt gcaccaacat ctggtattgt gatgggagct cttgcacttg gacgtatcaa
    16621 cattggtact tggtggaaat tcatgggcaa actcgtagtc gctattattg tagtgaccat
    16681 cgcccttctt ctccttggaa ccttccttcc attcctataa aatagtgagt gaggtgattc
    16741 catgaaaata gatataacaa atcaagttaa agatgaattt cttatatcat taaaaacctt
    16801 gatttcctat ccttcagtac tcaatgaagg agaaaatgga acaccttttg gacaagcaat
    16861 ccaagatgtc ctagaaaaaa ctttagagat ttgtcgagac ataggtttca ctacctatct
    16921 tgaccctaaa ggttattacg gatatgcaga aatcggtcag ggagcagagc ttctggccat
    16981 tctctgtcat ttggatgttg ttccatcagg tgatgaagca gattggcaga caccgccatt
    17041 tgaagcaact atcaaagacg gctgggtatt cggacgtggt gtccaagatg ataaaggccc
    17101 ttcgctcgca gctctctatg cagtaaaaag cttgctggac caaggtattc agttcaaaaa
    17161 gcgcgtacgc tttatctttg gtaccgatga ggaaaccctc tggcgctgca tggcacgcta
    17221 caataccatc gaagaacagg ccagtatggg ctttgcacct gactcatctt ttcctctgac
    17281 ctatgctgaa aaagggcttc tacaggtcaa acttcatggc ccgggatcgg atcaactaga
    17341 gcttgaagta ggaggcgcct ttaacgttgt accagacaag gccaactacc aaggtctcct
    17401 ctatgaacag gtttgtaacg gtctcaaaga agctggttat gattaccaaa ccactgaaca
    17461 aaccgtaacg gttctcggag tgccaaagca tgctaaggat gctagtcaag gtatcaatgc
    17521 tgtcatccga ctagctacca ttcttgctcc tctccaagaa caccctgctc tcagttttct
    17581 tgcaacacaa gcaggtcaag acggcacagg aagacaaatc tttggtgata tagcagatga
    17641 accttctggt cacctatcct ttaatgtcgc aggtctcatg atcaatcatg aacgttctga
    17701 aatccgtatt gacattcgga ctcctgtctt agctgacaag gaagaactag tagagttgct
    17761 tacaagatgt gcacaaaact accaactccg ctacgaagag tttgactatc tagcgcctct
    17821 atacgtcgca aaagacagta aactcgttag cacactgatg caaatctacc aagaaaagac
    17881 tggcgataac agtcctgcta tttcatccgg tggtgccact tttgctcgca ccatgccaaa
    17941 ttgtgtagcc ttcggcgcct tattcccagg agcgaagcag acagaacatc aggcaaatga
    18001 atgtgccgtt ctagaagatt tgtaccgtgc tatggatatt tatgccgaag ccgtctatcg
    18061 acttgcaact taatcaggca actgtttcta caaaaaaaaa tcgaccgatt aatgaactgc
    18121 accccaaaag ttagacagaa taaatctaac ttttggggtg ttttattatg aaattgaatt
    18181 atgaagataa agttcagatc tatgaactaa gaaagcaagg acaaagcttc aaacagcttt
    18241 caaaaagatt tatgtggatg tttctggttt aaagtcatct gaatctttga gatgagcttt
    18301 ataaatcgct tttttcagtg tttgcactgg tgtttcgata aactcaaact ttttagccgt
    18361 ggtattgcct gattttatag tatattgaaa ctagaatagt acacctctac ttctaaaaca
    18421 tttttagaaa tcgatttgtc ctgttcttat ttcattttac tatatttgag ccacttcgtc
    18481 tttaacggct ttattcataa gctcttgtaa tttttcttta ctatcaatta ctcctgattt
    18541 tccgttgtaa tttattgtaa taggttttaa cttacctaat ttctcgacac gctcattaat
    18601 ttgatctttt tttgaaggct gcatatgttt ttcctaatat tttttcgaaa atatatttat
    18661 cagatagcgg tttgccttct tcttcagctt ggtttttgta ttgatttgaa acataaggaa
    18721 caaatccttc atagtaacct aatgctccca taagttcaaa agcttgtttt ctaattcaaa
    18781 ccattgcaac tcagatttca gcttttcaga taaatcctgc tcatccaaat aatgacttga
    18841 aattagtgct gaactcgttt ctatatcctg tacaagctga gcacccatac cagcaaaaaa
    18901 taaactcgtt cctagcaaga ccgaacaagc tcctattgca tatttcctca aagaaaaacg
    18961 ctgctttctc tcaaattgaa attctttcat cccatctccc atcattcatt attactgtat
    19021 attttgtata tcagaaatag tttgtattca caaatctttc tagttattcc cttatcattc
    19081 ctaattaagg gagataacat acaataattt ttagttaaat gtatatcgat gttttttgtt
    19141 tttcttaata aacgcaatac aaaaagagcc tgttgccaag ctctttatat tcaatgaaaa
    19201 tcaaagagca aactaggaaa ctagccacag gttgctcaaa acaccgtttt gaggtagtag
    19261 ataagactga cgaagtcagc tcaaaacact gttttgaggt tgcagataga actgacgaag
    19321 tcagtaacat ctatacggca aggcgacgct gacgtggttt gaagagattt tcgaagagta
    19381 ttagtctatt atttcttctc agcgcgaagg gctgacaaga tttgtgttcg gatatcatcc
    19441 acaccatttg gagtatttgg taaaaagatg gtttgatttc ctttagaggc aaaggtattc
    19501 aaggtatcca aatactggtt ggtcaagagg atagacatga tttgttcttc tgtcatgcca
    19561 acattggctt ccttgagttc ggtgatagac tctgccaatc catccacaat cgccttacgt
    19621 tgttgggcaa tccccacacc atgaaggcgg tctttttctg cttctgcttc agctgcagtg
    19681 acaattttaa tcttgtcagc ttccgccaat tcttgtgctg cgacccgctt acgttgcgcc
    19741 gcattgattt cattcatgga ttgcttaact tctgcatccg gttcgacttt ggtaatcaag
    19801 gttttcacga taatgtagcc gtaagtggtc atttcttctg ctacttggtg ttgaacttca
    19861 agggcaatct catctttttt ctcaaacaat tcatccaagg ttaattttgg aacagaagag
    19921 cgaagagcat cttcgatata agatttaatc tgagattctg gacgtatgag tttatagtaa
    19981 gcatctgtca cgctctgctc gttgacacgg tactgagtcg ctacattcat cataacgaac
    20041 acattgtcct tggtcttagt ctcaaccaca atatcacttt gcaacaagcg caactgaatc
    20101 cgtgctgcaa tcgagtcaat cccaaaaggc aagcgaatat gaataccgct attagcaacc
    20161 ttttggtatt tcccaaagcg ttcaataatc gccaccgact gctgacgaac cacataaact
    20221 gtactcagtg tgactatcac caataggagc acacaaacaa tcagaaaaat catgaaaaat
    20281 attgccataa tggaacctcc acaagtattt ttctagtatt atagcacatt taaagaaggc
    20341 tgtgccgttt ttactgcgat ttttcctgaa atgtcaataa ttagaggtga attgtcctat
    20401 tgttgtccaa tctcttgcta aaataactct ttataaaagg caatcgtttc ttctaaggtt
    20461 ggcataaatg gatttcctgg tgcgcaggca tcaatcaagg cattcttaga aaggtattca
    20521 aagtcgaaat ctttttcttc aataccaagt tcagtcagtt tcttaggaat acctactgtc
    20581 tcagaaagct tctcaatctc aacaatcgca taatcggcac attcttgatc tgatttacct
    20641 tctacatgaa gtcccaaggc tttggcaaca ttgcggaaag cttctggtac acgtttagca
    20701 ttttcacgtt ctataactgg tagcaacatg gcacagcaaa caccatgtgg caaattatat
    20761 actgcaccga gttggtgagc cattgagtga acataaccaa gaccagcatt attaaagctc
    20821 ataccaccaa ggaagatggc attgaccata ccttcacgcg cttcaatatc atgtccgttt
    20881 gctacagcac gaggaagata ttccttgata agctcaatag caccaataga cagtttcttg
    20941 gttacatcat aagcaccagg tgttaccaaa gcttcaacag cgtgagtcag agcatccata
    21001 ccagtagcag cagtcaggcc tttaggtttg gaaagcatga gttccgggtc attaacagag
    21061 ataagagcaa ggctattctt gtcaaccatt accatcttaa ccttgcgttc ttcatcagta
    21121 atcacatagt taatggtaat ttctgcagaa gttcctgccg ttgtattgat agccacaact
    21181 ggcaagcctt ttttagcaga cttatgaagc ccttcataat cctgtggttt tccaccattt
    21241 gtagccatga tagaaataca actagctgca tcctgtggag aacccccacc aagactgatg
    21301 ataaaatcac atccatgttc ttgcaaagca gctaccccat ctgtgacatt cttacaagta
    21361 gggtttggct ctacatcgct aaagatgaca tattcgattc cttctgtatc cagtggtttt
    21421 aaagtcttag gtaaaatatc actgccttcg atgtacttat ctgtcaccaa aagtgccttt
    21481 ttatagccaa gttccttgat ataaggaccc acttcattta caacaccttt tccaataagg
    21541 ttgactgccg gaacataaaa tgtagccata taattttcct cctgcgcctc cgcgctatct
    21601 atttaacatc attatattac tttttgtatt cgtttttaat tatttgtgaa ttgtttaaca
    21661 aatcttttta aaaggatagg agggacgtaa gttcacctcc tatataaaca agttttattt
    21721 atgtagtggt cccaacaact gacatgcacg atagtctgct gattctagat cttctgttcc
    21781 aaataaggac caatttttag gtctaaagat atcttcctca ggtacattat gcatattgac
    21841 aggaattctc aacatagaag ccaaggtaat caagtctgct ccaatgtgtc catatgttat
    21901 ggctccgtga ttagctcccc aattattcat gacgtcatag acagacttga aagcaccttt
    21961 tcctgtcaaa cgtggagcaa accaagtagt tggccatcct ggatctgtac gattatctaa
    22021 agtatggtga acatcttcag gaagttcaag tgtataacct tctgcaattt gtagcactgg
    22081 accaacccct tttagaagat tgagacgtac cattgttact ggcatatccc ccttcgtcaa
    22141 gaaacgagtt gagaatcctc ctccacggaa gtattcgcgg tttgctggtg gaaagtctgt
    22201 attttcaagc atagcctgca cttcactttc ttccaactcc cagaatggtt tcataacagg
    22261 tttgccatct cgagtagctt gacctgtacc atccaatgta caagaaccag aattgattag
    22321 atgtaagaag ccagctgcag cacgaccctc taaagtatgt cccgttacac gtttaacagc
    22381 ctctgggctc caataagtac gcacatcagc aaagatttgt ggagtatttg ttaatagata
    22441 attaaagagc atagacacac catttagtga atcattctct gtcgcaaata caaatggttt
    22501 tcgaatacca ttccagtcaa actgagtatt gaggaaagtt tccataaagt caccatttgg
    22561 aaaatggtct gtccactggc gttgaccttg gaaaccagct actaaagcat ggtgaccaac
    22621 tgcttcttcc tcaaaaccaa gttcagcaag tcttgggtta ccaaccatta agtcacgtcc
    22681 aatcatgaac atcttaataa caaattccca ttgtttttct ttctcttctt tagaaagaat
    22741 caaatcctca cgatttcggt caaccccttc tttgatatgt tcttttaccc aaaccatagc
    22801 acgttcgaac tcttctgggt cataaatacc acggtccata cggcgcgtga actccgtcat
    22861 atctaccgat tcatttcgca ttcctaagta ttcttggaag aaatctggat ttacaataga
    22921 accgccaatc cccatcgaaa cactacccat tgataggtaa gcagtgtctc tcatcaagcc
    22981 agttgcaaga gctgcacgcg catagcgtaa gagtttttct tttacatctt ctggaatagc
    23041 tgtatcacta gcttcctgga cgtctctacc ataaatccca aatgctggaa tccctttttg
    23101 agcatgtgaa gctagtacag ctgcaagata gacagctcct gggcgttctg tcccattaaa
    23161 tccccaaata gcatgaggaa tatctggaga catatccata gtttcactac cataacacca
    23221 gcatggtgta actgtaattg ttgcgcaaac atttgatttt ttaaacaact catgtgaagc
    23281 tgctgcctct ggaacacgac caatagtaga tggagaaatc acgcattcca caggttcccc
    23341 atctggatat ttcaatgtgc ttgaaatcaa atctgccaca cttttagcca tgttcattgt
    23401 ttgtacttca agtgattcgc gtacaccttg acgacgacca tcaatagtcg gacgaatccc
    23461 aatacgtgga tgttgaatca tactattttc ctcttatttt tctgtttctt tcatactacc
    23521 gatgataatt tttaaaatta cttataagta taaacctcta cctctgccaa acttaaagga
    23581 attcctgcgt ttctcaaaga aattctaacc ttttttccct tcagcccttt taagtctatg
    23641 gatagattat tgctttctaa actgtcaata tgttgtttaa aaacttcatg attatttgaa
    23701 tcatatataa taatatcaaa atttgataat ctctgaattt cagcatctgt tctattataa
    23761 ataattaact tttctaattc ttcggtttgt gctaaatcta tctcccacca tgaaggagaa
    23821 tctcctttgg tatgagtcac tgaatgatga ccataatctc cgtttttatt tccatcaact
    23881 gctagacgag aaaaaccatt gtaatccgtt gaactctgtc gagtttcttt ggttaatgca
    23941 atattagaaa gtttcttcgg agttctcttt gaaccataga cttcaacttc cgctaaactt
    24001 aacggaatag ctgctgattt taattctatt ttgactagct tagcccctac agaaggtact
    24061 tttaaagata acaaaccatc aactacttta tcaaaatgtc ttctaaaaac ttcttctcct
    24121 gatgaagata gaaaaataac atcaaaatta gataaacgct gaggttcggc atctgttcta
    24181 ttataaatat caactttaga aaccgtaaac tcttctccca gatcgacctg ccaccaagcg
    24241 ttagaatctg ccttagtatg agtcaccgaa tgatgtccgt aatctccatc tttattacca
    24301 tcaactgcta aagcagctac accctcataa ttggttgaac tttgagtagc taccttattt
    24361 gtagagacta ctttatcttc tgttatttta tctatatcct cttcagattg cttaccatct
    24421 gattctctaa aaacctctac ttctgctaaa ctcaaaggca ctccactcgt tagtaattta
    24481 actttaatat accttgctcc tttttctttg aaatctagac taacagattc acccgacaaa
    24541 ttattaacat gtttcttagc aacttcgttt ctattattgt catatagaat cacatcaaaa
    24601 ttagatagac gttgagtctc agcatctgtg cgattataaa ttttaacaag cccaacttta
    24661 tccatttttt tcaaatcgac ttcccaccaa gagggattat ctgccctagt gtgtgttacc
    24721 gaaccagagt taaaattacc atttctgtta ccatccacag ctctattagg gtctccattg
    24781 taatctacag aactttgtgt tgttggtttt gcatatgcta tattcaaatt accatcatta
    24841 aatttatctg gtgttgggaa agggactggg cctgtccctt cagtatttat agacatctct
    24901 accattccat tatgattaac cgtaatggta taaacatggg tattctcatc ccaattttct
    24961 gtataagtat aatgattttt atcgccactt atatttatct gaggtttatg accagtatgc
    25021 ccttttaatg taagtgttgt tgtcctaaag tcattatcta caggattgat ggaataattt
    25081 ttgcttatcc agttcgctaa ctctaattct tctttcttcc aactttttga tgcatcaaaa
    25141 tttcctgata atgcccacat agctgtctta tctgtcctgt aattgttcaa taaaatatgt
    25201 aaattatttg gattttcttt aacaacagcg taagtatgtg gcgtcaaatc taacgataac
    25261 gactttgtat tattagtata cataggcaac attacttgct gatttttatt gatattagca
    25321 ttactattat aaatatacca ggaattacct acacgctgag catacccatc tccttcataa
    25381 agttttggat aaagcgagtt taaatagttg actttactag acaattcctc actattttta
    25441 gtcaaaattt ttgcattagg gaatatagat gaaatctttt ccttatcaat tttctcatgt
    25501 attacaggaa gaatatgata tctcccatta ttatataaag gcattgtttc atcattcgaa
    25561 taaagtcctt gataaaatcc gtttaatgaa ctaatcctac cttctccatt ccaaaataca
    25621 gcttttgttc tatttacaac ttcttcctta cttggagctg gattttgtat agcatgtcta
    25681 aagaaaggaa taataccttt agtaaatgct ggagttggta catcatttgt cataaatgta
    25741 tacgcggcac attcaaaatt ataaactgtg cctcccccag tatatacatt catcatttcc
    25801 atagcaatca ttgattctgg ttccgatgca taggatctca tatccctagc tcttccagtt
    25861 tcaaatgtgt ttgtataatg tttttcccac catttccatg tatctgttga tgagccccag
    25921 ttatcacata agccactcaa ccaaaatcca ctaacgatag aatctgtacc cgcatcatct
    25981 cttattggcg tatttttagt tgccaacacc aaatttttat gatatttttg actcgcttca
    26041 aagaatgtcg gatcattcat aatagtttcc cagaaccatt tttcatgatc atgccagata
    26101 aaatgcgctc catatttggc acaaacttcc aaatatttag cactatgcgg agctaactgg
    26161 ttattgtaaa tccaataatt ctcaatattt aaaacacctt ttaacacact atacttttgg
    26221 aattgttcat ctaaccactc tggaggaact gtattacgct ctccagccga cataataacc
    26281 aagaaaactg gaatgtttag actttgagct tcctccaaca tcttgacata ccattctctc
    26341 aattctttcg tatctcgtgg aatacaactt gttggtttac agacttttgc aggatgaagt
    26401 tcaatcgctg catatggctt tacatcttca ggaatagctt cccatgctcc cttcaatgtg
    26461 ttcccccacc ataatccgtt accattatca ttgccataca acggagaaag caacaatggc
    26521 gattcattat taatcgtcgt tctcatttga acacgattat cagccttaac tcctgctaac
    26581 aatccaaaac ataacatccc aatacataca aacagtattg tgattaattt tctttttatt
    26641 ttttctttat tcatgactta tacctaaaaa tttaactcaa taattttttc tgaattaaat
    26701 accaattcaa tattttgtac atcagtattt ttgccatata ttcttactct tactttttga
    26761 tctttgtttc ctccttccaa ttttaggaat gttatatccc catttttcca agcaaacgat
    26821 accttatatc ctcctcttac tctgaaacct ttcacttctc cttctgacca agcagaaggt
    26881 aaagctggaa ttagtgataa ccaattatga tggctctgta ctaataattc acaaattcca
    26941 ctcaccaaac ctaaattacc atcaatttga aatggtggat ggtcaagaaa taaattgcca
    27001 agagtcgcat tatttaacaa accattaatc tggttataag caggttcacc ttgatatagt
    27061 ctcgcaaaaa aatgaatcag ccatgcagca ctccaacctg tttgtgtact agcatgcaaa
    27121 ccacttacta accaattatt aatcgcttgc tccctatcct gtgaagataa aaagttagcg
    27181 tttgataatc tcctattgat agtgatttta gctgcttctg ctaattccgg agttttatga
    27241 atatcaatct cattataagg ataaagccca aatagaggtg aaatgtgtct atgaccaggc
    27301 tctacttctt cataatcctc tagccattct tgtatttgcc cattactacc tatttttgtt
    27361 ctaggtaatt tcttttttaa ctccttcaca cgactaataa aatccgaatt gtctcctaat
    27421 tgttttgcaa ttccaatgca tgaatcacaa aaatatctta aaatttgatt atcaattgta
    27481 gatgatagac aagcatttcc ttcaatacca ttttttaagc gatatttatt ttccggtgag
    27541 acacttggac ctgtcatcaa gtagccatcc acctcaaata aataatcttc aaagaaaaga
    27601 aatgcttctt ttatcatttc aaaatgttcc gtaagaatac gctcatcttg gaaatataaa
    27661 tagtgttccc aaatatgagt acataaccat ggaatagtta atacccaaat tgcagccccc
    27721 atggcatgag attggggagc cgtatcgcca aaaccatccg tattatgatg tgctgtaaaa
    27781 cctctagctc catacatttt cttagcggtt agtcttcccg gttctctcat tctttcgagc
    27841 atatcaaata atggatattc tacttctggt aaatcacatg gacctaccat ccaataattc
    27901 atttgagtat taatattaat cgtatattta gaaccccaaa ttggatttaa ttcatcacac
    27961 catattcctt gaagattggc aggtaaacca ttcggttgac tagacgatat taacagatat
    28021 cttccataat gaaataacaa gttagtcaag tagttactat actttttagt gttttcaaga
    28081 agtagattcg ttggaatgct aagacaatct ttactatagt ctagtttaaa atcaactcta
    28141 ttaaattgct cctgatattt ttttacatgt tcatcttttt ctgtaaagta atcaatacta
    28201 ctaaattctc cctgaagaga agaaatatct atatttcccc aataatccgt cattgatttg
    28261 agataaagaa atacctctgt agcattccga ataactattg tctctcccaa tacacttact
    28321 tcaccatccg taaccttaga atgacatact actttaaact gaacaccttt tctacctcca
    28381 gcagaggccg acattaaaat tgtacttgaa tccagtttag atacttcgtc attaaaccgt
    28441 ttatttctac ccaaattaat gtttaaattt aatgtgtttt gaactgatga cactatacgg
    28501 caacataaaa tattcttatt aaaactcgta aaatattctc tttttatttg taaattacaa
    28561 ctattaggct caaacacaac attagaaata gctgtatcta aatctagctc tctttcatac
    28621 aatgaaagag cacaagactg aatatctata tgctcaatgt aaagttcccc aagtaattca
    28681 tagtggcttt gatctcttgg ggtagcaaac attgttaact ttatcaattc ttcggctttc
    28741 tgaatttctc catctaaaag atattcccga atttttttaa gatgcaatag tgagtctgga
    28801 ttatttctat ctgactttcc tctataccaa atagtctcat cgtttagttg aatacattct
    28861 tttgtagctg aaccataaat cataccacct aaatgaccgt tcccaatcgg caaggcttca
    28921 ttccaatttg cggcagcttt cttataccat aatttcactc tttacctctc ttccaaaatg
    28981 gaggtgatat cccaaatacc acctccacac tttctactac ttcaaaattc ctaaaacaac
    29041 taggataact ccaatagcca taacactaaa gattagtaca accggattct ttccttttct
    29101 aattaattta cacattaata aagtcaaagc catagggata aatcctggaa ttaattttgt
    29161 aatcatttct tgaacagaaa taacaagttc cccctgctta aattctaatc caaaattaat
    29221 accaaccatt gatggaatca aaccacccac tagtactagc cctaatgctg tcgccatact
    29281 cgtaacgcga ttcaatgttc cttttgtatt attttcttgg ataagactag aacccttagt
    29341 atacccatat ttcaaaccga aatatttaac aggaatatta ataatattaa acaatattaa
    29401 ggcgataaag atacctaaag caccgccgtc tttagaataa gacgcaccaa tactaaaaca
    29461 gataggaact agtgtcagcc agaacaaact atctcctaga ccagccagtg gccccatcaa
    29521 gccaacttta ataccagtaa ttgaagctgc accttcattt ccttcttgtt cttccatagc
    29581 ggaagtaact ccaagaataa atggtgccgc tgtttgatga gtattgaaaa actcaaggtg
    29641 acgtttcatt gcaggagaag ctgaatcttt gtctgggtat agttttttca atactggaag
    29701 aattgtatat agaaaaccta ggttctgcat tctctcatag ttccatgaag aaccatacat
    29761 aaaacttcgt ttgaaaactt ttttaagttc aactttagtt actttattat tcatcataga
    29821 tcaagatcct cctcaacatc actacttgaa gttgtttctt gtggtttact tccaatcata
    29881 tcgtagaagt atgctaccgc aataccaact agtgagatcc caatggttga cattcctcca
    29941 tacacagaac aaatgaatcc aatcaacaag aatacccaca tatttttctt gagcatcata
    30001 tttaaaagca taccaaaacc aattgaaggt agtaaagcac ctgcagagtt aagtccctgc
    30061 ataacgattg gtggaacctt agcaaacaag tctgccacat attcccctcc aagcataata
    30121 gctaggaaaa ttggtacaat tttaaataaa gtgattaaaa ccaaacttga ataatgataa
    30181 aaaccgatct ttgaataatc tccgttttta gcatcattat cagctttttt cacaaaataa
    30241 acatctaaag tttttgcaag aacatccaac tgttgagtag caactgcaat tggaactgct
    30301 atagcgatac cagcagtttc tccttgacca gacaaaatac caaatgcagt accaataatc
    30361 gcacctgaaa tagtatctgg tggagtgtaa cctccaatac ctgttacacc gagccaagtt
    30421 aattcaagag ctgaaccaat aagaacacct tgggtgaaat ctcctaagac taagccaacc
    30481 attgcacttg taactaacgg acggtgaatc ataaataacg gtccattata gtcaatagtg
    30541 gcaataatcc caactaatgt caccagtaat gcttgtgtga atagcatata ctgtacctcc
    30601 ttaaattatt tttataaaaa tttttccaac attgtaactt tatcattagg aaccatttga
    30661 atttcaactc tcgttccctt atcaactatt ttcttaaaat aatatttgtc ttcttcacct
    30721 agagctacac ctcccgttat cttttcattt tcctccgttt tactcatctg tcctacattg
    30781 aggtactcca attttaaatt tccttcaata gaatcataca catcttttgg atttgtatat
    30841 atcagcattg ttctcttttt tattggcaca gagtttaaaa cttccacaaa ccgttttaca
    30901 ctaaagaaaa caatttttaa acctaccggt gcagaaatct ttaaaataga ttgtcgtgtt
    30961 ttatcttctg agatgcgatc attaacaatg ataacttgct caatatcata cttttttagc
    31021 cacgtagtga caacttgacc atgtaccaga cggtcatcaa tacgaacaaa ctctatagac
    31081 ataaatcctc ttcctcctct acgttaagtt gttgtttaac attaaacaaa ctattttgag
    31141 cattttgaac aatttcttct aaattgattt ttgaatcata acttgatatt aattccacta
    31201 ggagagggat attaaaccct gttacaatat caactgaatc caaatttaaa aaccgtgaca
    31261 aagccacatt attaggactt cctccaatca agtcagtcaa aacgataacc tcttgatttg
    31321 agtctaacag ttcatttact tgatttttaa aataatgttc aaactctaca atattctctc
    31381 caggcatcaa acctaatgtc ctaactctat catttgttgt ctcaccaaca aacatttctg
    31441 cggtcatgag agctccgcta gcaaaattac catgactggc aactaaaatt cctctattaa
    31501 acattttctc tttcaacaac tactcctttc ttaaggataa tattagcata aagtgaagtt
    31561 tctcctgtag caacaatagc ataagctttc ttactacgtt cataaaagtc ttctcttcta
    31621 agataagtaa tcgttttaag atctgtacca tgaccttcaa tcatctgtct ataggtaccc
    31681 catatcttag gaatatcatc acccgaaaca acgttcataa actgaattga actatcgacg
    31741 taactatcta atggcattaa atacagaatg gaatctaata attctggaat atttacacca
    31801 tcacaacgaa ttaatttatt cgcacatgag acggaagggt aattcgcgtc agctaatact
    31861 atttcatctc catgtcccat ttccattaaa gtcttcaata aatctggaga aatatttttc
    31921 ggtatatgtt ttaacattaa tatcctccct attttctctg accgtatgtt ttaaattttt
    31981 ctgccatcaa ttccatctcc tcatcaggaa gaactactgg ctctccaaaa ttcttagcta
    32041 aacaataaat ttttgcacaa tattcaactt cttcaacaat attaaatgca ttcaataaat
    32101 tttgtgcacc tgctaaaatt ccatgattcg ctagtaaaac tgctctacga ccttccattg
    32161 ctttagctgc attcacagcc aattctttcg taccatatgt tgcatactca gctacccgaa
    32221 catctttccc tgccactgca atcatataat gactcgctgg aagtggttct ctgagacaag
    32281 ctaatactgt tgcataagtt gtatgagcat ggataattgc atcgatatca tcacgagttt
    32341 gatattgaat caaatgcata taccattcgc tagatggcaa gcgttctccc tctacaacat
    32401 ttccattaat atccatcact acaatatcgg attctttgat ttcaaagaaa tcaatacccg
    32461 acggggtaat tgccatcaat tgtttttcac gatcgaaaac gctgagattc ccacctgttc
    32521 ctttcgtcaa atctgtttct actagcttct taccatattt aattaattct tgttttacat
    32581 ctgacatcct tgtcctcctc atatcaaaac actaaatatt tttcaattca ccaattatcc
    32641 ccccttcctg cacaaaagct aagaaggggg agggaggcaa atttacaaac tctcaatctt
    32701 tttaattctt ttgggagtaa taactctcta attgtaggaa ctcctcaata acatggtgag
    32761 cttcttccat cccttttaat tgtcccatag ctataagctg cacaacaata ttccccacag
    32821 ctgtaccctc agtcgaacct gtaagaacct ctttaccagt tctatcagca atcatttggt
    32881 taaaataact ggctctagca cctcctccaa tcacatatat cctcttataa accttatgag
    32941 ttagttcttc tagtaactct atcgctttcc tatacgtttc agctaggctt tcataaacaa
    33001 tcttaaatag ttgtccatct gtccactctc tagtttcatg atgataagct agatattctg
    33061 tcaaagtctt gtgcatatca gattctgttg caaattcagt tgattcagta tcaatcagag
    33121 gaagattttc tttttctttc tccaccattg tcctaatatc atcaaaagaa taggctttcc
    33181 ctcttcgttc aaatgaacgt cttagttcct ctataatcca caaccctgta caattcttca
    33241 gaaatgtaat ctctccatct ttaccgactt catttgtaaa tccataactg aaggattcgg
    33301 tagtaagaat cggtgaagta agttccactc caaccaaaga ccaagtacct gatgaaataa
    33361 ataaactacc ttctgtctta ggtactgaga caatcgcact tgctgtatca tgactacaaa
    33421 cattcacaac aggaatatcg cctaaaccat actccttttt tatccttcca agaacatttc
    33481 cctctgaaac aatttcagga agtaaagatg aatccaattc aaatagtttt aagatattct
    33541 gattccaatt ttgactccta ggatcaaata attgagttgt tgaagcaatg cttttttctg
    33601 tagcaaactt acctgtcaag agataattaa acaaatctgg cattaaaaga atcttattgg
    33661 tcttatagaa agagtcagga gattcttgac gtgccttaaa gagttgaaac aaggtattta
    33721 tctccataat ctgatttcct gtctctgaat acagtttttc taattcagtc atttcagata
    33781 tttcctttaa cactcccttt gttctttcat cacgataatg aacaggttgt gataacagct
    33841 taccttcatt atcaatcagt ccaaaatcaa ctccccatgt gtcaataccg atagataaaa
    33901 tcttgtaact agtattagcc aagcggatac tttcaagaat tttagctagt agaaagtcaa
    33961 catcccaaga taaatgccct tttactctaa taggtagatt agaaaagcga tttatttctt
    34021 ccattactag tttattttca gaaaggtaac caacgattgc tcttccagaa gtcgcaccta
    34081 aatctattgc taaaaccgct tccatttttc ttctctcttc gtccttgatt aactttatta
    34141 taatccccac gttttatttt ttcaatatgt gaacgcttac ttgaataatg ttcaaatgtt
    34201 aacccaatat tcataaaaat aattcaaatt cctccaattt gtgctataat atagttgaaa
    34261 tgataaatta ggaggagctt atgataaaag atgaacgtgt acttgaattg attgaaatta
    34321 ttaaaaagaa aaaaagaatt gccgtaaaag agctggcaga aatcactttc tccagcacaa
    34381 gtaccttacg tcgtgattta attttcttag aaaatcaagg tcttatcaaa agaaagcacg
    34441 gatacgtgac cctgtcctct atgaacacaa ttgaactttc tcatcaaata cgtgaaggag
    34501 aaagtactag gcaaaaaaga ctaatcgcta gtctcgctaa agactttatt cggtctggta
    34561 tgtgtatcta tctggattcc agtacgactg tctacgaact ctgtccctat ctttctgaac
    34621 ttgataattt gattattttt acaaatggtt tacatactgc acaaacccta tctgaaactg
    34681 ttaaagatag ctccaaaatc tttatcacat ctggcgaggt caaacatcaa tcctgttccg
    34741 tggtcaacta tgataaggaa aattctttat tagatcattt taatatcgat ttagcatttt
    34801 gttcagcaag aggtattgat gaccaatatg tttatgaagc ttctctcagc caagctattt
    34861 caaaaaagaa tattattgac aaagcccatg aaaccatctt actgattgat agttctaaat
    34921 tttacaagac tggatttttt aaaattaatc ccctctccaa atacacaacc tttatttcag
    34981 acaccgtgcc agatcaaaaa ttattagacg cagtcgaatt atttgatgga gaatgggttt
    35041 ctgatattca atgaaaatca aataggaagc tagtctcagc ttgctcaaag tacagatttg
    35101 aaaactgatc aagtcagctc gaaacactgt tttgaggttg tagatagaac tgatgaagtc
    35161 agtcacatat atacggaaag acgaagctga catgatgtga agagattttc gaagagtatc
    35221 gttctgtctg ctgactgatt tacaaatatt caccacatga cattattttc ttaacaaccc
    35281 caaaaacaga ggctggacaa aaactcaatt tcaggagaaa atggagtaaa ccttcccaca
    35341 ataaaacgca tagtatcaag tttttgcaca cctgatatta tgcgtttttc tgattttaaa
    35401 gatttttctc ttagatctga tatttcaaga attaagtgac cttgatgatc taaatgtatt
    35461 gtggtatact atttttgtca tcggttaccg attacgttcc tatgggttcg tgagaggagg
    35521 tgaaactaat gagccttatc atagagctcg ctcttactat tatagcggat gttatagctg
    35581 gaattatctt gtatttcgtc tgcagatggc tagatagtaa ggagtagacc gactgactag
    35641 cctataaaca cccgttaaat cgctaagata cgtcaaaaaa gcccttaact acggccatag
    35701 ttaggggctt tggtgttcta atgaacctta tcaattaact acattttagc atataatcta
    35761 caatagttca agcattaagt gaagttgatg atctaaattt agtgtggtat aatacttttg
    35821 tcatcggtta ccgattacgt tcctatgggt tcgtgagagg aggtgaaact aatgagcctt
    35881 ataccagagc ttgctcttac tattatagca gatgttatag ctggaattat cttgtatttc
    35941 gtctgcaaat ggctagatgg taagaagtag accgactgac tagcctataa acacccgtta
    36001 aatcgctaag atacgtcaaa aaagccctta actatggcac tagttagggg ctttggtgtt
    36061 ctaatgaacc ttatacacta actacattct agcatataag cccagatatt tcaagagttt
    36121 tatttatttt ttcaggttcc cttagttctg aaaggtctat aatgaagtta gccatctagt
    36181 atcaaaaaac cgactagctc ttatgaacta gtcgatttct cattaatgcg ccaacatttc
    36241 ttgggcgatt tcttggccag ataggttatc tggatagtag gttggccaat tatccatttc
    36301 ttcaaataga gcttcttggc ttgtgcctcc aaagaagata tggaaatgtt ctgccttaac
    36361 tggggcaata ttgtggtcac taaactgaac atacttgaac tgtccagcgt cagcatctgt
    36421 ggcttcaaag aggaaacgca cgccacgatt gcctttcttg taagtcaaaa ttttcttacc
    36481 gacatacttg taagtgtatt tcttgctttg tccaccttga acaaattcca tagtattatc
    36541 agtaatgtta atcttagtca catctgtctg atagcctttt gtatagtaag ccttgtactc
    36601 agcctgggtc atcttaccag tcaacttagc cttgtagtca aagacttggt caaacgtgcc
    36661 gtcttcaagg aaaggataaa ctgattgcca gttacctgca tagtcactca aggtgcggtc
    36721 cttgacagct gcatcctcga agtaaccatt ttggactgtc ttggtatcct ctgccttttc
    36781 aggttcgatt gctgggcctt cttggtctgt tgtttgtttc aaagccttga ggtttttctc
    36841 catcacggaa atgtagtttt ctccagcctt ggtgtcctct tctgtcagac tttctaaagg
    36901 attgaggaca tcagttttga cacctgcttc ttttgaaagt gtgttagcaa gggcttgtga
    36961 ggcattttct tcaaaataga tataggcgat tttatttttc ttgacatact ctgtcaattc
    37021 tgccaagcga gcagctgatg gctctgcatc tggagaaagg cctgagattg cgacttgttt
    37081 gagtccatag tccaaggcaa gatagttaaa ggctgcgtgt tgagtcacaa agctcttttg
    37141 ttttgcttga gacaagcctt ctgcgtaagc cttatccaag gattgcaatt tttcgatata
    37201 ggcagctgca ttcttctcaa aggtctcttt tttatcagga taatctgctg acaagctgtc
    37261 gcggatgtgc tctactagtt taatggcacg aactggtgat aaccaaacat gggggtcaaa
    37321 ctcatggtga tgaccttctt ctccatggtc atggtctccc tcttcttcct cgccacctgg
    37381 caagagcaac atatcgcctg tcgccttgat ggttttcact tttttcttat ccaaggtatc
    37441 tagcaattta ggtacccatg tttccatgtt ttcattttca taaacgaagg tatctgcatc
    37501 ttggattttg gcaactgcct tggcagatgg ttcgtattca tgaggttctg tcccagcacc
    37561 gattaggagt tctacattag ccgtatctcc tgcaacttgc ttggtaaatt catagacagg
    37621 gtaaaaggtt gtcacgatat tgagtttacc atctgcctgt ttttgattgg aacaagccac
    37681 taaaaacaag gcacatagac tggctagtaa taagctaatt tttttcacgt tcgtctccta
    37741 tttgataaaa cgtcttacta aactgattag tataaagaca gttacaaaaa taatggtaat
    37801 acttgcactt gcaggtgttt ctgcatagta ggaaatgtaa agtcctgcta ccattcccaa
    37861 aaagccaatc gcactggcaa gcagcataac cgatttaaag tttttcccca gacgcagggc
    37921 aatactagct ggcaagacca taatggtcga taccagaaga gctcctgctg caggaatcat
    37981 aagggcaata gccacccctg tcaccatgtt aaaaagaatg gacatggtac gaactggcaa
    38041 gccatccaca aaggccgtat cttcgtcaaa agttaagata tacataggac gaagaaagag
    38101 aaaggtcaaa atcaaaacaa ccgccgcaat gacaaagagg gaaatgacct gttcttcact
    38161 gatagtcacg atcgaaccaa agagatattg gtccaaactc attgaactcg agcttttacc
    38221 cttgctcatg acaatcagag aaacagccag acctgttgac atgaggatag ctgtcccgat
    38281 ttccataaag ctcttgtaaa ccgtacggag atactccaga aagaccgccg caatcaagac
    38341 aatggcaata gtagaaacag ttggagaaat ccccaaaacc agaccaaagg ctacacctga
    38401 aagtgagacg tggctaaggg tatcactcat caaactctga cgacgcaaga tgaggaaggt
    38461 tcccaatacc ggtgagaaaa gactcatagc aataaccgcc aaaaaggcgc gttgtataaa
    38521 gtcgtaagat aataaactaa gcatggccca cctcctggcc attctcatga acattgaaac
    38581 aacgccatgg cgagtcttgg ttacggacta gatgaatatt gcgatccgca taatccttaa
    38641 cttcttcagg gtcatgggta atcatcaaaa cagccttgcc atgatgatgg gcgctgtggt
    38701 gcatgagttc gtaaaattca tttttacttc ctgcatccat ccccgttgtc ggctcgtcta
    38761 ggataaacac atcagggtca gaagcaaaca tacgcgcaat taccgctcgc tgcttttgtc
    38821 ccccagatag agaccccaag cgtttgtctc gatgttccca catgccaact gagtccagac
    38881 tagccttgat atgctcctca tcatgagcat tcaaacgacg gaaccagcct tttctcggat
    38941 agcgacccga cttgacaaat tcatagaccg tacttggaaa accagcatta aaactggcaa
    39001 tttgttgagg aagataggct attctcaatt tcttaccttg cgtatttgtc tttgaaatag
    39061 ccacctttcc aatgcgtggt tgcagaattc caagactagc cttgatgagc gtcgtcttag
    39121 ccgctccatt ttccccagtc aaggtaacaa attccccact atcaacacta taattgatat
    39181 gttcaagaac aggctcctta tcataataga aggacaaatc ctctaccgta atatatctca
    39241 ttatttgatt tctcctacta aagcagtcaa aaaccgctga atcacttttt gttcatttgg
    39301 agtaaactga gtcgccactt gttcataggt taaaagtgta tgctcatggt gatggtggtg
    39361 ctcctcagcg attggacgag ccaagtcagt caactgataa aaaatcacac gcgcatcttt
    39421 agaatcttta gatgtttcca acatcccttc cttgaccaaa gacttaatgg ccttggtaac
    39481 tgccgcctga ctgacattga gacgacgggc caattctgaa tttgttaaag attcctctga
    39541 caagagcata aggatatgct cctgagtatt ggtcagggcc acctcgctag tgcaatgacc
    39601 tattaggatt tcatgctgat tttccgcctg caaaatcacc tcattcaaaa aagcattgat
    39661 atcctttgct agctgtctca tatctgactc ctttcctttt agacttctct tttttaagag
    39721 aaaaatacta ttctttgaca ttttgtttac cagttaatta tatcacaagc aaaaaaagag
    39781 tcaagaaaaa acgtgaaaac tagtttcatt cttgaactct tctatattat attatctatt
    39841 gaaattcttt gacatctcca tcataagtcg cccaatcttt gctgaaaaaa cgctcattca
    39901 gatggtaagt cggagctggt gtgtgattgg ataggaaagg atcaactgcc ttgtcaaaag
    39961 ccaaccaacc caaccaacca aggtgaatgg tgtccttcat aaagaaaggc tccccgccgt
    40021 ccttagaaaa atctgctata ttggtaaaac cttgactttc taactggtag cgaatcttct
    40081 gcaccgtttg ttggtacata tcctctcgta gaccagcata gtccatccat tttttattaa
    40141 caggtggaat gataaaaatc gggtttacct tagatttaga aaactgtgtt aaaaccaact
    40201 gcaagtcatt atactctggc gacttgagat aggtaaagct tttctgagaa tcctttaatt
    40261 tcttcaaatc cttcttgatc tgctcattat agaaataatt ttccattccc atctcattat
    40321 tggaagtatt tttttcagca tctgctttga caacatcttc tattgcttga taagaaaact
    40381 ggtctggcaa gatttttaaa tacttagcta catgcttatc gtagttaaca tagcctctaa
    40441 ccgaaaactg accaaaaaag gaggcttggc gttcattaaa acgagccaat aattcaatca
    40501 tttcattgtc tgctgtcgac aattcttctt tacttgccaa cttctgaacc aggtccttca
    40561 tagctacgtt tgggaactgt tgcagtaagc gagtcgctgc atattgacta gcctgatccc
    40621 cagattgatg tttcagaaaa ctagtcaact ggtctccatt aaaatactgc tggaaggctg
    40681 ctggatcata gccattttta ctgaaccact gaggtgagat aacatacaca acttgtttat
    40741 tctccagctg tggtaacatc tgttgcattc caaaatattg gttaagcgat gcagctcccc
    40801 cctgtcctaa aagataagga cggtaggaac gattgtattt ctcagctaat accgcaggat
    40861 gagcaccatc aaaacgaagc cattcactag agccaaagaa gggaacaaaa cgcacatttg
    40921 gatcagatag tgctctgact ttttgacttc gctccttaaa actatcgata gtagtagcca
    40981 ctgctgaacg cttttcagct cctagattat gatgcatctc agtaggataa aagaaaatga
    41041 gcagaaaaac caacaaacca gcgatcaaga ccggtccgaa gatcatccat aagcgtttaa
    41101 gcattttgta gctccacaat accagctatg attttattag ctgtattcca gtcgtcacga
    41161 ccaaactctg ttacagggac acgaatgtca aaacggttct caatctccac aatcaactca
    41221 accgttccca tactatccaa gacacctgca tcaaaaagat cttcatccat catgtcagaa
    41281 acatcttcca taaacaactc atcaataatt tcaataactt ctgatttgat atccatattt
    41341 tatttccttt tattttttaa accatagatt attcaagaat ccagaaaaga ttaagaatga
    41401 caacatgaca acatggaaag tgacaaccat gccaagcaac tgaatccagc gattctcagg
    41461 tagggcagcc ttccctgctt ttttccgttc cttattgagc gtttttttct tgcgaaccca
    41521 ggcatcattg atgaccaagc ctagtccatg aaagagtcca taggcgatat agtaccaggt
    41581 cacaccatgc caaaatccca taaccagcat atttacaatg taggccatgc ttgaggttac
    41641 attacgattt ttaaagactt tctttctggt taacaccatc accattcgca taaagacaaa
    41701 gtcacggaac cagaaggaca gactcatatg ccagcgattc caaaactcct ttaaatccct
    41761 tgataaaaag ggcttgttaa agttgatagg gctacggatt cccatcaagt ttgagatggc
    41821 caaagcaaac atagaataac ctgcaaagtc aaagaagagc tccagaccaa aagtatacat
    41881 aactgccaag gcatagagat taaagaagcc acctgactgc aaggctaaat tcttcagagg
    41941 aggtagtaag gtctctccta aaacatgagc taggataaac ttatacaaaa agccccgcat
    42001 gatatagcgg acagattcat ccagcatatc catcaactca tctcgctcag gaatggcctg
    42061 ataattttca ttaaatcgct taaagcgatc gattggacca ctcgagaaag ttggcatgaa
    42121 aagaaggaaa cggaggaatt cccagagggt aaaatcctta atcactccat ctctcagctc
    42181 gatgataatc ccaaccgaac gaaaggtcag gtaagaaatt cccaagaacc caagcaaaga
    42241 ctgcgttcca ttgatagctg gttgcacctt gacaaagata atcggaagta gggacagaaa
    42301 actaactaag tagaagaccc acttgccatc cttgcttttt cgataatgct tgtagaaaag
    42361 caggagcaat atttcccagc aaaggtaaat acccaaggca gctagttgat tggtctttcc
    42421 acccaccaac atggtgacaa taaagaagag acttaccaac acttcatacc aggcaaagcg
    42481 tttcttgaaa aagaggccta taaagatggg caaggttgca gcaatcacat aaacaaaata
    42541 ctgaggattg ccatatggct ctaaatgagg aagctgttga aaaaactcca tcatctctta
    42601 ttcacctcgt taatcaatcc tttgatgtca atctttccat ttggagtcag tggcaaactg
    42661 tctcggtaaa ggaatttaga tggcatcata taggacatca tgatgtctgt caggtcttcc
    42721 ttgatggcct tggtaatatc gatatctcgc tcaaactgct cacgaacacc gtcttttaag
    42781 atgacataag ccaatagatt ttgtaccttg tggtccttgt tatagcgcgg tactgcgaca
    42841 gcagattcga taaagcgaga cttgttgagg ttttgagaga catcttctaa ctcaatgcgg
    42901 taaccgttaa acttaatctg gaagtccatg cgtccgccgt agagaagcaa gccctcatct
    42961 gtcatggttc ccacatcgcc tgtgtgatag gctggcagac cttcaaactc aaagaaggct
    43021 tctgctgttt tttcaggatt gttcatataa ccttttgaaa cagctggccc agaaacaatg
    43081 atttctccct gttcgccatt tggcagttta tttccttcct cgtcaatgat aaaggttgga
    43141 gaatcagcct tggtatagcc gattggtagg cgtttgagag tcgctaacat ctcgtctgtc
    43201 acggcaactg ctgacagagc tactgtcgct tctgttgggc cgtaagcatt gatgatacgg
    43261 gcatttggga aacgctcgcg cagtttttga gctgttttga ccgtcaattc ttcaccatca
    43321 aagtagaaat gcgtgattcc aggcattttc tcactgttga agtattcaga caacatggcc
    43381 atatctgcaa aggatggtgt tgatgtccag atagcgattg gcaatgaaaa gatagccgca
    43441 aagagttgct taaagtcctg agtgatgact gaaggaagag tgaaaagcgt accaccaagt
    43501 gccaaggtcg gtgcccaata catgacagac aagtcaaaag aataaggtgg ctgtgccagc
    43561 atttgcggac gactcggtgt cgcaaattcc ttatccgtaa tcatccagtt tgtaaagctg
    43621 aggagattat catgtgaaat ctgcactccc ttaggcttac cagtcgtacc agaagtaaag
    43681 ataatgtagt aattatcatc tcccttgact ggatgcgtga tttcatagtt gttcccttgg
    43741 gcaaaggctt cttgaacctg agctagattt atcattggtg tagaaacctg ctccaaggga
    43801 aaggctgaaa tggcaataat caagcttggc tctgctactt ctaaaatagc tgaaactcgc
    43861 tccaaggccg aatggctatc aattggaatg taggcatgac ctgacttagt cagcgctaca
    43921 aaggttgcca acatttcata ttcttggcca ccaaaaacaa ccacaggaga cttctcaggc
    43981 aagcctagtt ggtcaatgac tgcagccaaa ctatccgaat cagcctttaa atcgccataa
    44041 gtgtgttcct gccccaaaac attatagaca ggatagctag gctgtgtctg agcaaaatgc
    44101 tcaatggttt caatcatatc tgctattggt ttatttgaca caatagggat tctccttcaa
    44161 gttaaaattc attatagata aagcttcctt gaccctgacc aagatagcta aagaagtaaa
    44221 gcagccctag aaagataaga aaatacaagg ctgtccgacc aagaaagagg tacaattctt
    44281 ttctctgttt catcaagaaa aaccattcat ttctgtaatt tttcgctaaa agctaaaata
    44341 agagtgattc ttactagctt atttttctac cattgtacca ctttatatag tatcttttca
    44401 attgtttacc gtatgtttcc aatagatttc agcttatttt aaggattata cagtttttct
    44461 atgtatattt tcaaatagag tgatcctgct tcaaaactcc atttcaggag acaatgaagt
    44521 aaatcttccc ataataaaac acacaatatc aagttttttc aacacctgat actatgcgct
    44581 tttctgattt ttaaagactt tttaaccact ctctcattta aaataatctc gtctgatata
    44641 aattaaaata gcttctatca tcagacaaat ggctgatagc caaaaactga tgctaatacc
    44701 aaaactctca gtaatatagc tcattagcaa aacaaatact gaaaatgcta atgtagaaat
    44761 cacttcaaga acggaataga cattaactaa atgattttcc tctactgttt cctgaagaaa
    44821 tacactttca ggaacttctt ttagttgcaa taacatacca actaaagctg aaaataataa
    44881 aaacatctgt gcgtttggaa aatatagaat agtcagtgtc actattgcca tagctacaag
    44941 aggaaaaaga atactttccc atttagcagc aaggaacttt tcagatagcc taaaagcaat
    45001 taagccacta attataatac caatagagta tgctgtatta gaatatcccc agtaactttc
    45061 cgttttattt aataactccg ttacaaaaac aagtataatg gaagaaaccc aaatcgtatt
    45121 tgaaaaaatt tccaataaat ttgctgatac aaaaagtctt aatctaggat ttctagcaac
    45181 taacttccaa cctttgagca aaatttcaag attagtttct gactctaaca cctccacttc
    45241 agcgttagga agaaataaca tcagaaagct agaaatgata tacaagacta aattgataca
    45301 cgtggtaggt aacagaccaa ttgttgcaaa caagagtcca cctaatcccc aacctatcaa
    45361 ttgaacagct tcaccagtca ttgataaggc tgaattagcc ttacccaaat cggtcgcata
    45421 gcgtggcaca atagcatagg aaacgggtgc tgcaaaacca tctagtatgg aaattgcaac
    45481 aacaaataga taggtcacca aaggcgctac ggattgcatt acggtaaaca ttcctaccag
    45541 tatcgccaat aatatagtct ttccaaattg agataaagat aaaaccctat ttagcgctaa
    45601 ccttttagta accaacggaa ctaaaagact cgcaacaaaa gaggatattc ctattaagat
    45661 aggaactagt gatgtggcaa ttactgattt tgaaataatg tatatgttag caatgattgt
    45721 tactctaaag aaaatatctg ctaaatttgc aaataattga gagactaaca acttgacaaa
    45781 tgaattagac atgtagcgct ccccctttag ttacaaactt tggaattaaa tttgattttt
    45841 aatattttag cataaaatag cagaaaacaa aagtgaggtt ttttacatga aaaaacaaaa
    45901 actattgcct ttattattcc taattttagg aggaattatg attatcgttg ttggctagta
    45961 ctaagaagtt agttagttag tagaataatt acatcttcaa tcgaaaaaga gactggaaaa
    46021 agtcttaaaa atcagaaaaa cgctcaaatc ctttggcgct ctaagctttt ctaatgatag
    46081 atttagatag tcaaaaaaat atctgaaaag ttcccaggat cgtcgtctca gcatcaaaaa
    46141 tacgtgaaat ttgcataatt aaaataaatt tcaggggagt taatcgagta ggggataatc
    46201 tctagcccct ctcacaccac cgtacgtgcc gtttggcata cggcggttca actaactttt
    46261 aacgcatgtc gttcaaggta ataatccaaa cacgaaacca gtccacgttt ttcaaggact
    46321 ggttttgata tagcacgttt aagtaccgac ttctgagcta
//
LOCUS       DAVDWT010000015        45899 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVDWT010000015 DAVDWT010000000
VERSION     DAVDWT010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45899
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.015"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            469..1830
                     /gene="trpE"
                     /locus_tag="VUW50_000964"
     CDS             469..1830
                     /gene="trpE"
                     /locus_tag="VUW50_000964"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET7982880.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETTSVQVTKYMEVELFRY
                     VMHLTSVVKGRLLPELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            1827..2393
                     /locus_tag="VUW50_000965"
     CDS             1827..2393
                     /locus_tag="VUW50_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET7982881.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEEVVK"
     gene            2404..3408
                     /gene="trpD"
                     /locus_tag="VUW50_000966"
     CDS             2404..3408
                     /gene="trpD"
                     /locus_tag="VUW50_000966"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET7982882.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            3405..4172
                     /gene="trpC"
                     /locus_tag="VUW50_000967"
     CDS             3405..4172
                     /gene="trpC"
                     /locus_tag="VUW50_000967"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET7982883.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            4159..4758
                     /locus_tag="VUW50_000968"
     CDS             4159..4758
                     /locus_tag="VUW50_000968"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET7982884.1"
                     /translation="MTKVKICGLSTKEAVETAVSSGSDYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            4736..5959
                     /gene="trpB"
                     /locus_tag="VUW50_000969"
     CDS             4736..5959
                     /gene="trpB"
                     /locus_tag="VUW50_000969"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET7982885.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGRDLPDALVACVGGGSNAIGLFHPFVEDESVAMYGTEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            5952..6728
                     /locus_tag="VUW50_000970"
     CDS             5952..6728
                     /locus_tag="VUW50_000970"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET7982886.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIKTEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIAGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(6700..6801)
                     /locus_tag="VUW50_000971"
                     /pseudo
     CDS             complement(6700..6801)
                     /locus_tag="VUW50_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            7030..7747
                     /locus_tag="VUW50_000972"
                     /pseudo
     CDS             7030..7747
                     /locus_tag="VUW50_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7762..8913
                     /locus_tag="VUW50_000973"
     CDS             7762..8913
                     /locus_tag="VUW50_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET7982887.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRASNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSICLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(8965..9624)
                     /locus_tag="VUW50_000974"
     CDS             complement(8965..9624)
                     /locus_tag="VUW50_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET7982888.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFILQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            9694..10140
                     /locus_tag="VUW50_000975"
     CDS             9694..10140
                     /locus_tag="VUW50_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7982889.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSRLKLE"
     gene            complement(10368..11546)
                     /locus_tag="VUW50_000976"
     CDS             complement(10368..11546)
                     /locus_tag="VUW50_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET7982890.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDNYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            11826..13088
                     /locus_tag="VUW50_000977"
     CDS             11826..13088
                     /locus_tag="VUW50_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET7982891.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFITGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            13091..13987
                     /locus_tag="VUW50_000978"
     CDS             13091..13987
                     /locus_tag="VUW50_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HET7982892.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNQEIIKAVQSAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            14002..14319
                     /locus_tag="VUW50_000979"
     CDS             14002..14319
                     /locus_tag="VUW50_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET7982893.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            14336..14653
                     /locus_tag="VUW50_000980"
     CDS             14336..14653
                     /locus_tag="VUW50_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET7982894.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEKTSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            14663..16018
                     /locus_tag="VUW50_000981"
     CDS             14663..16018
                     /locus_tag="VUW50_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET7982895.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMAYKTEL
                     S"
     gene            16341..17816
                     /locus_tag="VUW50_000982"
     CDS             16341..17816
                     /locus_tag="VUW50_000982"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET7982896.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            17813..18661
                     /locus_tag="VUW50_000983"
     CDS             17813..18661
                     /locus_tag="VUW50_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET7982897.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            18676..19446
                     /locus_tag="VUW50_000984"
     CDS             18676..19446
                     /locus_tag="VUW50_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7982898.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTTKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(19724..20011)
                     /locus_tag="VUW50_000985"
     CDS             complement(19724..20011)
                     /locus_tag="VUW50_000985"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET7982899.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            20320..20592
                     /locus_tag="VUW50_000986"
                     /pseudo
     CDS             20320..20592
                     /locus_tag="VUW50_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(20703..20906)
                     /locus_tag="VUW50_000987"
     CDS             complement(20703..20906)
                     /locus_tag="VUW50_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET7982900.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(20937..21545)
                     /locus_tag="VUW50_000988"
     CDS             complement(20937..21545)
                     /locus_tag="VUW50_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET7982901.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            complement(21584..21754)
                     /locus_tag="VUW50_000989"
     CDS             complement(21584..21754)
                     /locus_tag="VUW50_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HET7982902.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            complement(21766..22332)
                     /gene="amaP"
                     /locus_tag="VUW50_000990"
     CDS             complement(21766..22332)
                     /gene="amaP"
                     /locus_tag="VUW50_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HET7982903.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            complement(22416..22580)
                     /locus_tag="VUW50_000991"
     CDS             complement(22416..22580)
                     /locus_tag="VUW50_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET7982904.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            23023..24504
                     /locus_tag="VUW50_000992"
     CDS             23023..24504
                     /locus_tag="VUW50_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7982905.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            24866..25867
                     /locus_tag="VUW50_000993"
     CDS             24866..25867
                     /locus_tag="VUW50_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7982906.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKP"
     gene            26019..26936
                     /locus_tag="VUW50_000994"
     CDS             26019..26936
                     /locus_tag="VUW50_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7982907.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            26947..27837
                     /locus_tag="VUW50_000995"
     CDS             26947..27837
                     /locus_tag="VUW50_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7982908.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            27866..29482
                     /locus_tag="VUW50_000996"
     CDS             27866..29482
                     /locus_tag="VUW50_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7982909.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVENLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            29492..30811
                     /locus_tag="VUW50_000997"
     CDS             29492..30811
                     /locus_tag="VUW50_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET7982910.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKIEQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKNNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(31021..31093)
                     /locus_tag="VUW50_000998"
     tRNA            complement(31021..31093)
                     /locus_tag="VUW50_000998"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31058..31060),aa:Lys,seq:ctt)
     gene            complement(31358..32629)
                     /locus_tag="VUW50_000999"
     CDS             complement(31358..32629)
                     /locus_tag="VUW50_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET7982911.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            32899..33138
                     /locus_tag="VUW50_001000"
     CDS             32899..33138
                     /locus_tag="VUW50_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982912.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVYSNNGLKKYEK"
     gene            33303..33482
                     /locus_tag="VUW50_001001"
     CDS             33303..33482
                     /locus_tag="VUW50_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982913.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTR"
     gene            33619..33798
                     /locus_tag="VUW50_001002"
     CDS             33619..33798
                     /locus_tag="VUW50_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HET7982914.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITIPHGE
                     LNKYTERGIRKQAGL"
     gene            33835..34287
                     /locus_tag="VUW50_001003"
     CDS             33835..34287
                     /locus_tag="VUW50_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET7982915.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            34578..35048
                     /locus_tag="VUW50_001004"
     CDS             34578..35048
                     /locus_tag="VUW50_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HET7982916.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPKY"
     gene            35067..36134
                     /locus_tag="VUW50_001005"
     CDS             35067..36134
                     /locus_tag="VUW50_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HET7982917.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYQLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVGITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKELAEGV"
     gene            36136..36294
                     /locus_tag="VUW50_001006"
     CDS             36136..36294
                     /locus_tag="VUW50_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-dihydro-8-oxoguanine-triphosphatase"
                     /protein_id="HET7982918.1"
                     /translation="MEIEISDFTGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     KSVRENLI"
     gene            36404..37438
                     /locus_tag="VUW50_001007"
     CDS             36404..37438
                     /locus_tag="VUW50_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7982919.1"
                     /translation="MKKSDVLDLIKYHYEGRETEFRNQSIAIARNFNKHGDTQIAQYI
                     MGLMSQSDRFMPQIENPSEYLTPAKLDIGPLPLPLSIMNDLKGIINAVNHHIGINKFL
                     FVGSPGTGKTESVKQVARLIGKELLVVDFSHLVDSKLGQTVKNLATLFNEINNLPFKQ
                     NYIILFDEIDSIVLDRVNQNDLREMGRVTSAFLKELDRLSPEIVLIATTNLFENLDKA
                     VTRRFDAIIDFDRYTDEDKVEVATIILNELLKQFKNVARDLKLFKKIINSANVIPNPG
                     DLRNSIRTSLAFSDPSDPHDYQKRLLRSLHNGRNLSISKLSKLGFTVREIEILTGISK
                     SSVSRELSED"
     gene            37500..39683
                     /locus_tag="VUW50_001008"
     CDS             37500..39683
                     /locus_tag="VUW50_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET7982920.1"
                     /translation="MGPITIPKKTIITLEHLKYLHFSLEETKTYWEKNNIIDGILISI
                     YYNRIVAKSNRINGYFNVGGGNPFPNDTIVGAKFNDEKTKHIVTHYISRDALNKTITV
                     LSKIIEVFEEHFDRAITCEMFSDSSTFASINFSEYGISKSKFQQYLRDSCFIENFGVE
                     HTTVSDIQNSIVTFYDVHTDIFRLLNKLNIDISEANIMNQTTVLLDEKNIELLLSKAP
                     YLVSMIVEDFSKLSVDDFSLDNNDLKINLPSPMNEPVVGVIDTLFDKRVYFNEWVEYH
                     DFVSPDISKDSQDYKHGTAVTSLIVDGANLNPNLDDGCGNFRVRHFGVSLQSGFNSFT
                     IIKQIKEIVSQNADIKVWNLSLGSNDEIRENFISAEGALLDEIQFENDVIFIIAGTNA
                     SVINGKRKRIGAPADSLNSIIVNSVDFNNQSVSYSREGIVLSFFVKPDVSYYGGGNGD
                     FINVCEPLGLGRVAGTSFAAPFIARKMAYLIHIMGLSREEAKALLIDAAIPWNDKKTF
                     TDLSLIGNGIVPIKMDDILSTPDDEIKFIVSDISRAYDTYNYDFPVPISSESYPYVAK
                     ATMCYFPNCSRKQGVDYTNTEMQLTFGRLKSDGIKSINKDNQHAEDTPGYVRENAARN
                     IFRKWDNVKHIGESFTSRKRAKAILNPSNPQWGMSIKTIERLKSGDGQGVRFGVVVTL
                     KELNGVNRIEDFIQQAELRGWLVNRLQVEAQVDLFNSLNEEIEFE"
     gene            39734..40750
                     /locus_tag="VUW50_001009"
     CDS             39734..40750
                     /locus_tag="VUW50_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET7982921.1"
                     /translation="MNITYIVGNGLDLQYGLKTRYRDFYEFQNKVYISRTENEEKYSN
                     FIYESLFSDKVNDYENWSDFELSIGKLTKDNDLISSSIEIKEKFIDDFSEVVDDLREY
                     LRIQQEKNLEKGNAIDFISTLDDMRTSLPVINQPAIDKKYNENPHQDDIVNIVTLNYT
                     HVIDKLYNGSAKSFRNQLRANLYNFYIEPPIHAHGTVDVCTVLGVSDEIQISNSLEEQ
                     KESLIKSLVLKNYRENMDVKNSDIIKNSDIIILYGVSLGETDRYIWSQIAERSISGSV
                     PVIIYHYVPHFDPGNPIRAKRLYRNVEDKFIQNSGIDLELEKKLRDNLIVVIGKTIFD
                     LIER"
     gene            40765..41715
                     /locus_tag="VUW50_001010"
     CDS             40765..41715
                     /locus_tag="VUW50_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET7982922.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTVLA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEMIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            41717..42460
                     /locus_tag="VUW50_001011"
     CDS             41717..42460
                     /locus_tag="VUW50_001011"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET7982923.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            42471..44267
                     /gene="pepF"
                     /locus_tag="VUW50_001012"
     CDS             42471..44267
                     /gene="pepF"
                     /locus_tag="VUW50_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET7982924.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDATLTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSGTGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            44513..45067
                     /locus_tag="VUW50_001013"
     CDS             44513..45067
                     /locus_tag="VUW50_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HET7982925.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRDDLSNQGL
                     HYLSKEKDTTGFSSQYGWVHSFAHGADLLTEVVCHPDFPKNRVHEVFDILGQLFKRMS
                     ILFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDELKEAIQSFQY"
     gene            45178..45846
                     /locus_tag="VUW50_001014"
     CDS             45178..45846
                     /locus_tag="VUW50_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET7982926.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT        14349 a         7569 c         9755 g        14226 t
ORIGIN      
        1 agatagaact gacgaagtca gtaaccatac ctacggcaag gcgacgttga cgcggtttga
       61 agagattttc gaagagtata aaggtgtacc gagatgaaca tctcttcatc aatgtctctg
      121 gtcgtggagg caaggaccta gctgcgattg cagattacta gaagctaaaa aataatagat
      181 ggaaaaatta cagttctttc tctcacagag agcttgtggt tgctgaaaac aagcagagaa
      241 agctgtaaag ttgggttcat ctgaaacgag agaagaaaac atcattctca agggagtgcc
      301 ctttatcgca cagcctgtta caggagatat ctgagacagg aatgagagat aggagaaatc
      361 ctataattga ggtggcaccg cgaatttcgt cctcacgcaa gttattttgc gtggggattt
      421 ttcatacagt caccacggac tagaagtaga actgaaaggg aaattacaat ggaacgaatc
      481 attcatggag atgtcttatc accaatcttg gcttatatgc gcttaaaggg gcaacataag
      541 gttattttag agagtattcc gagagacaag gaaacagctc gtttttctat cctagcctat
      601 aatccagttt ttgagattaa gtttgaaaat ggggttctct atcaaaatgg tcaagtgatt
      661 gatcgggatc ccttggattt cctttatgaa gtgattcata agagtcagca ccactcagag
      721 ctaccttttg gtggtggagc cattggtttt gtgggttacg atatgatttc gctttatgaa
      781 gaaattggtc aaatccctga ggatacaatt gggacgccag acatgcattt ctttgtctat
      841 gagagctata tggtctttga ccacaagaag gagaagatac atgtcataga ggatgcactt
      901 tatagtgagc gcagtcaaga ggccttggaa aaatccttga accaagtgct tgaggaatta
      961 cgcattcctg ctccaaatga atttgaagat ttggatctat ctccgttaga cttcaaaccg
     1021 catatcgctc cacataagtt tgagggaatg gtagaaacag ctcgtgactt gattcgtaac
     1081 ggagatatgt tccaatgtgt actcagccag cgtttttcag cagaagttac tggaaatcca
     1141 tttgacttct acagaaatct ccgcgtgact aatccttcta attaccttta tttctatgat
     1201 tttggggatt atcaaatcat cggagctagt ccagaaagtt tggtttctgt caaaaatggc
     1261 atcgtgacaa ccaatccgat tgcagggacg cgaccaagag gggctacgga tgaagaagac
     1321 aaggccttgg cgactgacct cctctctgat gagaaggaaa cagcagaaca tcgaatgttg
     1381 gtagacttgg ggcgtaacga tattggccgc atctctgaaa cgaccagtgt ccaagtcact
     1441 aagtatatgg aagtggagct cttccgctac gtcatgcatt tgaccagcgt agtcaagggg
     1501 cgtttgcttc cagaactcac tgccatggat gccttgaaag caacgcttcc tgctggaacc
     1561 gtttctggag caccaaagat tcgagcgatg agacgtatct atgaactgga aacggaaaaa
     1621 cggggagtat acgctggggc aatcggctac ttgtctgcga cgggtgatat ggatttggcc
     1681 atcgccatcc gaaccatgat tctcaaaaat caaagagcct atgtgcaggc tggggcaggg
     1741 attgtctacg actctatcgc ccaaaacgaa taccaagaaa ccattaacaa agcaaaatct
     1801 atgactagaa ttggagaact aagaccatga ttttattgat tgacaactat gattctttta
     1861 cctataactt ggcccaatac attgggaatt ttgcagaagt tcaggttctg agaaatgatg
     1921 attccaagct gtatgaagaa gctgaaaaag cagatggtct ggtcttttct cctggtcctg
     1981 gttggccagt tgatgctgga aagatggaag acatgattcg tgattttgca ggcaagaagc
     2041 cgattcttgg gatttgtttg ggccaccaag ccattgcaga agtctttggt ggtaagctag
     2101 gtttggctcc aaaagtcatg catgggaaac agagcaatat caactttgaa gcgccatctg
     2161 ttttgtatca aggtattgag gatggccgtg cggtcatgcg ttatcacagt attttgattg
     2221 aggaaatgcc agaagacttt gaagtgacag ctcgttcgac tgatgaccaa gccatcatgg
     2281 ggattcaaca taaaaaccta ccgatttatg gcttccagta ccatccagag agcattggaa
     2341 cgccagacgg cttgtcttct attcggaatt ttatcgagga ggttgtaaag tgaggaaact
     2401 aggatgaaag agattattga aaaactagca aaatttgaaa atttatcagg tgtggaaatg
     2461 acggatgtca ttgagcgtat cgtaactggg cgtgtaactg aagcgcagat tgcttctctc
     2521 ctcttagctc ttaagatgaa gggggaaaca cctgaagaac gcacagccat tgcccaagtc
     2581 atgagaggac atgcccagca tattccaact gagattcatg atgccatgga caactgtggt
     2641 acaggtgggg acaagtcttt cagttttaat atttccacaa ctgcagcctt tgtcttggct
     2701 ggtggcggta ttcacatggc caaacacggt aaccgctcga tatcttctaa atctggttcc
     2761 gcagatgtcc tcgaagcctt gggaatcaat cttgacctca aaccagctga actaggtaag
     2821 gtctttgata aaactggaat cgtctttctc ttcgctaaaa atatgcaccc agctatgaaa
     2881 tacatcatgc cagctcgttt ggaattggga attccaacga ttatgaactt gactggtcca
     2941 ctgattcatc caatggcttt agaaacacag cttcttggaa ttagtcgtcc agaactccta
     3001 gaaagtacag ctcaggtttt gaaaaatatg ggtcgcaaac gtgccatcgt ggttgctgga
     3061 ccagaagggt tggatgaagc tggcttgaac ggaacaacca agattgcact tcttgaaaat
     3121 ggcgaaatca gcttgtcaag ctttactcca gaggatttgg gaatggaagg ctatgctatg
     3181 gaagatattc gtggtgggaa tgctcaggaa aatgcagaaa ttttgcttag cgttctgaaa
     3241 aacgaagcaa gtccattctt ggaaacgaca gtcttgaatg ctggtcttgg tttctatgct
     3301 aatggtaaga ttgatagcat caaggaagga gttgccttgg ctcgtcaagt gattgctaga
     3361 ggcaaggccc ttgaaaaact cagactgtta caggagtacc aaaaatgagt caggaatttt
     3421 tagcacgaat cttagagcag aaggcgcgtg aggtggagca gatgaagctg gagcaaatcc
     3481 agcctctgcg ccagacctat cgcttggcag aatttttgaa gaatcatcag gaccgcttgc
     3541 aggtaatcgc tgaggtcaag aaagctagcc ctagtttggg agatatcaat ctcgatgtgg
     3601 atattgtgca acaggcccag acttatgaag aaaacggagc agtgatgatt tcggtgttga
     3661 cagatgaggt tttctttaaa gggcatttgg attatctacg ggaaatttcc agtcaggtag
     3721 agattccgac gctcaacaaa gactttatca tagatgaaaa gcaaatcatc cgcgctcgca
     3781 atgcaggtgc gacagttatc ttgctcattg tggcagcctt gtccgaagaa cgcctcaagg
     3841 aactgtatga ctacgcgaca gagcttggtc tggaagtctt agtggagact cacaatctag
     3901 ctgaactaga ggtagcccac agacttggtg ctgagattat cggggtcaac aaccgcaact
     3961 tgactacctt tgaggtcgac ttgcagacca gtgtagattt agccccttac tttgaggaag
     4021 gtcgctatta catttctgaa tctgccattt tcacagggca ggatgcggaa cgactagccc
     4081 catactttaa cggaattttg gtagggacag ctcttatgca ggcagaaaat gtggcccaga
     4141 gaatcaagga gttgcagatt gacaaaggtt aaaatttgtg gactatcgac caaagaagcg
     4201 gtggaaacag ccgtttcatc aggatccgac tacatcggtt ttgtctttgc acctagtaaa
     4261 agacaggtga ctttagaaga ggcagctgag ttggcaaagc ttattcctgc agatgtgaaa
     4321 aaggttggag tatttgtttc accaagtcgg gtagaactgc tggaagcgat tgacaaagtt
     4381 ggcttggact tggttcaagt tcacggtcag gtggcagatg atttatttga gaatttgcct
     4441 tgtgccagta ttcaggctgt gcaggtagat ggaaatgggc atgtccccaa ttctcaggca
     4501 gattatctac tctttgatgc ccctgtggca ggaagtggcc agtcctttga ttggggtcaa
     4561 ctggatacga ctggactagc acagcccttc tttatcgcag gtggccttaa tgaagataat
     4621 gtagtaaaag caattcaaca ttttactccc tatgcagtag atgtatcgag cggagtggag
     4681 acagatggac aaaaagatca tgaaaagatt agaagattta tagagagggt aaagcatggc
     4741 atatcaggaa ccaaataaag atggatttta cggaaaattc ggcggacgtt ttgtcccaga
     4801 aacattgatg acagcagttt tggagttgga gaaggcctac cgtgaaagtc aggcagaccc
     4861 aagtttccaa gaggaattaa accaactttt gcgccagtat gtgggacgtg aaactcccct
     4921 ttactacgca aaaaatttga cccagcatat cggcggagcc aagatttatc tcaaacggga
     4981 agaccttaac catacaggag cccacaagat taacaatgcc ttaggacaag tttggcttgc
     5041 caaacgcatg ggtaaaaaga aaattatcgc agaaacgggt gctggtcagc acggtgtggc
     5101 aactgcaact gctgcggccc tctttaacat ggaatgtacc atctacatgg gtgaggaaga
     5161 tgtcaaacgc caagccctca atgtcttccg catggagctt ttgggagcca aggtcgaggc
     5221 tgtaacagat ggttcgcgcg tgctcaagga tgcggtcaat gcagcccttc gttcatgggt
     5281 ggctaatatc gacgataccc actatatcct tggttctgcc ttggggcctc atccatttcc
     5341 agaaatcgtt cgtgacttcc aaagtgtcat cggtcgagag gctaaacaac agtaccgtga
     5401 cttgacaggt cgagatctgc cagatgccct agtagcctgt gttggtggtg ggtcgaatgc
     5461 tatcgggctc ttccatccct ttgtagaaga tgagtcagta gctatgtatg gaactgaagc
     5521 agcagggctt ggtgtggata cagagcacca cgcagctacc ttgaccaagg gtcgtccagg
     5581 tgtccttcac ggttccctca tggatgtgct ccaagatgcc catggtcaaa ttcttgaagc
     5641 cttctctatc tcagcaggtt tggactatcc tggtatcggt ccagagcatt ctcactacca
     5701 cgatattaaa cgtgccagct atgtccctgt gacagacgaa gaagccttgg aaggattcca
     5761 actcttgtct cgtgtggaag ggattatccc agccttggaa tctagccatg ctatcgcctt
     5821 tgcggtgaaa ttggccaaag aacttggacc agaaaagtct atgattgtct gcctatcagg
     5881 tcgtggggac aaggatgtgg ttcaagtcaa ggaccgcttg gaagcagatg cagcaaagaa
     5941 gggagaagct catgcctaag acactaacag aaaaattaaa tgctattaaa gcagctggaa
     6001 aaggaatttt tgttccctat atcatggctg gtgaccacga gaaaggtttg gacggtcttg
     6061 ctgaaacaat tcacttttta gaagatttgg gtgtctctgc tattgaagtg ggtattccct
     6121 tttcagaccc tgttgcagat ggacctgtta tcgaagaagc tggcttgcgc agtttagccc
     6181 acgggacctc tacccaggct ttagttgaaa ccttgaaaac cattaaaaca gagattccac
     6241 tggtcatcat gacctacttc aaccccctct ttcagtacgg tgtggagaac tttgtcaaag
     6301 atctggctga tacagctgtg aagggcttga ttatcccaga cttgcctcat gaacatgcca
     6361 actttgtaga accatttttg gcagatacag atatcgcctt gattccttta gtaagcttga
     6421 ccacaggaat tgagcgccag aaagagttga ttgcaggggc ggagggattc gtctatgccg
     6481 ttgccatcaa tggggtgaca gggaaatcag gcaattaccg tgcagatttg gacaagcact
     6541 tggcgcaact tcatcaagtg gccgacatcc cagtcttgac aggttttggt gtatctagtc
     6601 aagccgatct ggaacgcttc aatgcggtgt cagatggtgt tatcgtcggt tcaaaaatag
     6661 taaaagctct ccaccaagga gagccgattc aggattttat cagacaagca gtagcttacc
     6721 aaaaataatc aaacaagtag caagtaagag gaaaggcacg aaaggaagtc tttccttttt
     6781 cttttgcagg agaaaggtca gttttatagt gaattgaatc tggaatatta cactacggtt
     6841 cctaaaatat tttcagaaag cgatttgaat ttcccaataa atttgttcag tttttaattg
     6901 attttactat agaagatata attttctaaa ttactctgac acagaacttt aagaaaaaga
     6961 taattattgt agtaatttct ttaagaaaat gatataattc attaaaaagt atttggagga
     7021 ggaagaatta tgttgaatca ggatctcttt gattcgcttg aggcacaaaa aattgtagat
     7081 actttgatga aagggcaaaa agattatgta gatgaacgtc tagaaaaaag agagacaatg
     7141 atagtgtcga atggttatgc atggacgcga cctaatcata ttgatactgc atttgcatca
     7201 gcagatttgt ttgagtataa attacaatta gcaggacaga cttggggata tttagaattt
     7261 gaaacaaata cagaaaaata tgggaaagta ttgttaatta taaagggtaa gaagcgactt
     7321 acgaaccaat ttcctttggt acaaaaaaat aagagtggct acttatttga atacacttta
     7381 tcttaatcaa cattcttcct acaaaaatga tgaagatagt cattcctttc caattcagat
     7441 ggagttagtt tctgatgaaa tgattcaaga aattgaacaa gctactaaaa attcgaatat
     7501 cgaaaaattt atgattttaa cttatgaggc ggactcagaa aacaatatta tatctgtaga
     7561 tgttgttatg cctgatgcac gaactggtca gctacacttg attcaagatt tgtctgagta
     7621 tattcaatca agttcgtacc atttcgaaga agctaaatat caagatattc ctaatttttc
     7681 agaattatcg gaaacagaag attttgaaat tattccaaga atagaaaaac aagaaggtca
     7741 aaagtagtaa ggagtatata aatgtttaat ggtcgggtat tgaaagaatt acggctgtta
     7801 aatggtttaa gtagagcaga attagctcag agaattaatt taacggaaca agccatttgg
     7861 cagtttgagt ccaacgaaac gaaacctaaa ttatcaacca aaatgcattt ggccaaccaa
     7921 tttcatgttg atttaactta ttttgaacag gaagaagaga gcattcgatt tgattcttct
     7981 gtaattgcct ttagaaatgc agacctagca acacggaaaa caatagatat tcaaactatg
     8041 tatttacata aggtagatag tttgattgat tattttgaaa gttttgtaat tatacctaat
     8101 attataattc atgacctaag taatgtagtg agtgaatctt atcataaggg agaatccatt
     8161 gaggaattgg ctctttatgc cagggaaaaa ttaggtattt caaaagataa tcatgatttg
     8221 ctttataaat tagaacgttc aggtatctat atcgtggaac gattaattaa tggccaagct
     8281 gatgcttata gcgcatggtc aaaattggga agaccttata ttgtgttggg aacgaataaa
     8341 tcatctgtac gtcgaaattt tgacttagct catgagctag gacatattct tttacataaa
     8401 tataaggata tgaatgaaga tggcgatcgt ttggagcaag aagcaaatta ttttgcatca
     8461 tgttttttat tgccaaaaga agagttttta gtcaaatttg aagagagggt tggcaagcgt
     8521 gccagcaatc ctgatagtta tattttattg aagtcggatt tgaatgtttc gatacaggct
     8581 ttagagtatc gagcttttaa gttaggatta ttgactccaa agcaacattc ttacttttat
     8641 cgtcaaattg cgcaaaaagg ttacaaaatg attgaacctt tggatgatca aatttttgtt
     8701 aaaaaaccaa gcaaagtaaa gagtattctg gacgtcgttt tgagtaatca tctagtcagt
     8761 ctagcgacta taatgtctaa acaaagtatt tgtttacagt ttataagcga aatattttca
     8821 gtcgaaatga aattttttga tcagtatcaa gaagatagaa gaacagatcg atttgataac
     8881 atcatccctt tgtacaaaag aaataattta taatttaact gtgaaataat ttcatgaagg
     8941 acatatggca gaaattggat tttatcagac aagcagtagc ttaccaaaaa taatcaaaca
     9001 agtagcaagt aagaggaaag gcacgaaagg aagtctttcc tttttctttt gcaggataaa
     9061 ggccaggata cccgtcgcag aagcaaactg aatcaagatc agtaactccg ttacgctaaa
     9121 gacgagagca caagaagcta aaaagaggaa atctcctgcg cccatgcgga tatcgataaa
     9181 atgagccaaa attccaagag caaggaagga gaccatgacc agattccagc cagaggaagc
     9241 tattaggatt aggtggaaag tcatccagac cagtaaggga tattcctgat ggtgaaagtc
     9301 gtagataccc aaggtcaaac cagcggtgat taggacgact tgccccaagg aaagcaattc
     9361 ccaagagtaa agcagaaaga ggagtcctaa gactaattca aagagggcat accagacagg
     9421 atagcgaact ttgcagtagc gacagcgaaa gcgattgaag acctgtgaga gaatcggaat
     9481 caaatctaag ggacgcaagc gagtctgaca ggaatcgcag tgactggctg aactgataat
     9541 ggattgctct ggaaaacggt caatgaccaa accaagaaag gaagcgagaa tgctcccgac
     9601 gagaaaaaaa taaaaatcaa tcatacttat ctattcgtaa aaaatgggag aaatagtata
     9661 atggagaaac atagataaga tggtgaggaa aagatgacaa ttcgttttga agaaaaggtg
     9721 agtatagaaa acgctcaatt tgtatgccag tggtccaact ccctaggcaa atcctttcaa
     9781 gaacaatgga tgggaccaag gattcctttt ctactgaccc ttcaagcctt ggaaggagtc
     9841 ttttcaatct ttgatgaaca agagtttgtg ggacttatcc agaaaatcag gctagaagac
     9901 agcaatcttc atatcgggag attttttatc aacccccaga aacaggagca agacttaggt
     9961 agccaggctt taaggaaatt tgttagtttg gcctttgaaa atgaagatat agatagtatt
    10021 tctctaaatg tcttcgaggc aaatcaaaga gctcagaatc tttaccaaaa agaaggattt
    10081 gaaatcgttc aaatggttga agcacctgta cgaaaatata gtagattgaa actagaatag
    10141 tacacctcta cttctaaaac attgttagaa atcgatttga ctgtcccgag cgattcgtcc
    10201 tgttattatt tcattttatt atacattatg aaaaagggga gataggagta taattccaaa
    10261 tataaagaaa gcgaacaaaa cagaattctg attttcagaa aacaggttta ttcgcttttt
    10321 atgatatact ccctcttttt agcctattta ttaaatgtaa tagaaaatta agaatgtaga
    10381 attgtatgat acaaacaaag tgcaatagct gagtgtgcgg ctgtaaaatc attgtaaggt
    10441 tcaataatga cttcaggtag gcgattatag agcagtttgg gtttgtagtt catttcacta
    10501 tatagttttt ggatgatgtg ttgatttgtt agtaaaggac tatgtaggta gattttttga
    10561 gagtcaatca tcatactgat atttaaaatg gtttgactaa gatagagtaa ggcttgatgg
    10621 ataagtgtga ttattccagt gtcacctaat tgataagcgg ttaaaataac ttggatatca
    10681 atatcatcgg catttttgac taaactagga agtagagaat agggagattg atgatataga
    10741 atttttgatt ttttgattaa ccaagattct ccagcaaaag tttgtaagca tcccttgcgc
    10801 ccgcagctac attcttctcc ctcagatgaa acaactgtat gccctatttc tccaatcatt
    10861 agatttcctt tcccataaat gtttccgtcg tagatataag aacagtgcat acctctagca
    10921 aaatgaaaat aagcaaaatt agggtcattc tgttggcgac taaataggcg tttacctata
    10981 gccatacagt taacattgtt agagaaaaac aaaggcttat caaagtggga ttgaatcatt
    11041 tctaagtcga tatgttgcca taaaggattg tttgttgtta ttttataatt atctaaatag
    11101 cgaccaggta gcgcgattcc aattgcttct atttcataat cagagcagtt atttaagaat
    11161 tgctttagtg tctggttaat gagttgattt cccttttctt ggattaactg tttagtaaca
    11221 atttctttct cttcctttaa gatatttcct agattatctc ctaaagcaaa agtgaaatgt
    11281 ttttcagata gttcacaacc aatgtagtaa aatcgtttag cttgaatgtc tagcaggatt
    11341 ttttttctcc ctacgcttgt atcgtgttcg tcttcaccaa gttctaagag aatattttct
    11401 ttaatcagat catttgtgat actgctagtt gttgcaggtg ttattcctgt tttagtagcg
    11461 atttcaattc gtgaaatggg acctaatgtg taaactgttt ccagtatttt agctcttagt
    11521 tgtaattgtt tcttagataa ggtcatatta gttttcttcc tagttgtcag attcttaaaa
    11581 tcctatgatt ttttgctatg gataaatgta gatgttaatc tttttaagct tttcattgct
    11641 tactggtctc tttttattat atattgataa taaaaaataa tcaaatattt acacaaatcc
    11701 ttgacaaggc tttcttatag ttgtaaaata taaataacaa aagataatca aataacaact
    11761 tgacgtcaaa aaataaagat aattgataac ttttgataat aaaatatatt taagaggaaa
    11821 aggttatgga aaaattatta caagaaaagt tacttccagt agcggctaga ttgggtaata
    11881 ataaagcgtt ggtttctatt cgagacggta ttactctaac tataccttta ttgttgatag
    11941 gttcattact aatggttatt gcaagttttc ctattccagg atgggaaaaa tatctgggtg
    12001 atattggtgt tgctgactat ttatggaagg gtgttgatag tagttttgga ttacttggtc
    12061 ttgtagctag ttttggtatc gcttacttta tggctagaca atacaaagtt gatggtattc
    12121 ctgctggtat tgtgtcatta tcatctttta ttacagttac gcctttcatt acaggagagg
    12181 ctggagcagg aatgcctacc gctttcatgg catcaaaagg tttatttgtt gctatgattt
    12241 taggattgat taatggctat atctaccaat ggtttataaa tcataatata cagataaaaa
    12301 tgccggatgg tgttccacca gcagtatcta aaagctttag tgccattatt cctggtgcag
    12361 tgactattgt tggttggttg attgtttatg caactttaga taaattaagt ttacctaatc
    12421 ttcatgaaat tgctcaagta gctttgggag gtccacttgg acttttagga aataatgtta
    12481 ttggtcttct tatcttaatt ttccttaata gtagtttttg gtttgtagga ttacacggag
    12541 gaaatgttgt taatgcagtt atgaaaccgt tatggttagc taatctagat gcaaataaag
    12601 tagcttatca aactggagaa actcttccaa atatttttac tagcgtattt atggataatt
    12661 ttgtattcat aggtggcggt ggtgcaacca taggtttagt tcttgcgtta ggatatttag
    12721 cacataagaa gaaagctagt aaacaactaa aaactttggc acctataact gttatacctg
    12781 gattatttaa tataaatgaa ccagcaatgt ttggcgttcc tattgttttg aacattttgt
    12841 tactagttcc atttatctta gctccaatgt ttaacttact agtagcgtgg ggagcaatgg
    12901 catcaggttt ggttccgcta acttatacag atccaggttg gactatgcca cctgttataa
    12961 gtggtttact tgctacagga agtatttctg gttcattatt acaaattgta ttgattgttt
    13021 tggatgtctt actttattta ccatttgtaa tagctattga aaaacggttt aagttattgg
    13081 aggattgaga atgaaacgtt taattagtgc aaatccatct gagatattac agatgaatgc
    13141 tgaagaatta aaacaaagta ttttagcaag tgaaggtaga gttgttctat ctgaaaatgt
    13201 agttactcgt gagacatttg ttggggatat aactaattct gaaattgcta gagcttttgg
    13261 agctgatatg attttattga attgtgttga tgtttttgag cctaaaattt atgctttgga
    13321 tagttcaggt gatgatgtta ttcatcgctt acaccagctt gttgcttgtc caattggtgt
    13381 aaatttggaa ccgattgacc catctgcaaa gatgctagag gaaacacagg aaattgttgc
    13441 aggtcgtgtt gctagtgttg aaacattgaa tcgaatagag gagttaggtt ttgactttgt
    13501 ctgtttgact ggaaatcctg gaacaggagt tagcaatcaa gagattatca aagcagttca
    13561 atctgctaaa gagaatttct caggtttgat tatagctggt aaaatgcacg gggcaggagt
    13621 gaatgagcct gtggcagagc tttctgtcgc agagcaattg ttggaagcag gtgcggatgt
    13681 gatacttgtt ccagcagttg gaaccgttcc agcctttcat gaccaagagt tgcgtgaagt
    13741 cgttgatctc gttcatagta agggtggtct tgtattgagt gctattggta ctagccaaga
    13801 aacatctgat acagatacta tcaaggaaat tgcacttaga aataaaattt gtggagttga
    13861 tattcaacat ataggtgacg caggatatgg gggactggca acagtcgata atatttatgc
    13921 attgagcaag gcaattagag gagtgagaca tacagtatct cgcttggcta ggtcagtaaa
    13981 taggtgataa aggagtaagc tatggctaaa gtaacaatta tgttagcatg tgcagcaggt
    14041 atgagtacaa gtctgctagt gacaaagatg caaaaggcag cagaagataa ggggttggat
    14101 gcagaaattt ttgcagttcc agctcctgaa gcagaagaaa ttgtagcaac aaaagaagta
    14161 aatgtgttgc ttttaggtcc tcaagttcgc tatttactag gggactttca agaaaaacta
    14221 aaagatagac agattcctgt ggcggttatt ccgatgacag attacggaat gatgaatggt
    14281 tctaaagttt tagatttagc tgaaagttta ttagactaag attgaggaga atgttatgga
    14341 tgaaagtaat ttagaatctg taatggggct aattatgtat ggtggggaag ccaaaagtaa
    14401 tgctatggag gctattcagg cagcaaaaaa aggggatttc tctaaagcca atcgaagatt
    14461 agctgatgcg aatgctgcct tattacaggc gcataaggct caaacagaaa tgttgacaag
    14521 agaggcacag ggggaaaaaa catcaattag cctcttgatg gtacacgcgc aagatcattt
    14581 aatgacgagt ctgacttttg ttgacttggc gaaagaagtg gtagaagtat acgaacgatt
    14641 tgaaaaaaat taggagtttg gtatgaatac aatgttggat aaaatgcaag aaaaactttc
    14701 tccaattgca atgaaagttg ggaatcagaa gtttttagtt gctttacgtg attcatttgt
    14761 ggggactatg cctgttatta tgacaggttc cattgctttg ttattaaatg cttttctagt
    14821 ggatttacca caacaatttc acctagaaag tattacgaaa acctttcagt ggcttgttga
    14881 catcaataat ttggtattca agggaagtat accaattgtt tccttgttgt tcatctattg
    14941 cttgggagtg aatatcgcta agatttacaa ggttgataca gtttctgctg gtttggtatc
    15001 acttgcttcg tttgtcattt cgattggaag tacagttaca aagagtttcc ctttggcaaa
    15061 tgtaggagat gtgaaattag atcaaatctt acaaggaatt gataatctag catttgatgg
    15121 taaaaatctg atggtaacta ttgggaatgt gattccagga aaccatatta atgccagagg
    15181 ctactttaca gccatgatga ttggattttt agcctctatt attttctgta aagtaatgaa
    15241 aaagaactgg gtaatcaagt taccagattc agttccccct gcgattgcta agccgttcac
    15301 ttctatcatt ccaggattta tggcgatgta tatagtagcc atcttgactt atgttttcca
    15361 tttacttagc aatgacttat tgattgattg ggtttacaag gtgttacaaa cgccattact
    15421 aggtttgtct caaagtttct ttgcagttat tctgatgatc tttttgaata agttattctg
    15481 gttttttggt ctccatggag gaaatgtatt ggctcccatc atggaagggc tgtttggagt
    15541 tgctatgtta gcgaatctgg atgctttcca aaaaggtgaa ccgattcctt atatatggac
    15601 aagtgggtct tttggggcgt ttgtctggtt tggaggatta ggattagtac ttgctatttt
    15661 aattttttca agaaacagtc attatcgaaa agttgctaaa cttggtctag caccagttct
    15721 gtttaatatt ggtgagccgg tcaattatgg tttaccagtt gtcttgaatc ctttgctatt
    15781 tataccattt gtacttagtc cggttttcat ggcaacggta gcttactggg caacaagctg
    15841 gggtcttgtt tcacctgtta cacaaaacgt tacttgggta atgcctccaa ttttatatgg
    15901 tttcttctct acagcatttg attggcgtgc catcatctta tcagttgttt gtttgattat
    15961 tagtgtcttg acttatttcc catttgtgaa aatggcatat aaaactgaat tgagttaatg
    16021 gtcttacttg cgttttttgt gataaaatag aaatagatta cgtgtaagat gagttaggtg
    16081 taggatttct tatctccact aaaattgtta ttagacaaag gtttatagtt tattgaagtt
    16141 ggaatagtac actgtaactt ctaaaacagt actagaaaat gatttgaatt tcctaatcga
    16201 tttagtcata ttttagttca tttttctata aaacataaca agaatgttat tgttttatat
    16261 agagagattg atatagaaag gggtgcagta aaggtagata tcttcttgtg cctcttttgc
    16321 catattagaa aggagatttt atggttcaga caaaacagcc aaatattatt ttgattgttg
    16381 tagatcaaat gagagcagac gccttatcct tgaatagtaa ggataagcta gttagcactc
    16441 caacattaga tatgatggct agtgtaggtt ataattttga aaatgcctat tctccagttc
    16501 cttcttgcgt tcctgctagg gcagctttat tgactggctt agaccaagat aaaagtggac
    16561 gagttggtta tcaagatgag gtgccttgga attttactaa tactcttcca aaagtcttta
    16621 aagatatggg ttatcaaaca gagtgtattg gtaagatgca tgtttttcct tccagacaga
    16681 gacttgggtt tgatcatgtc ttacttcacg atgggtatct acatgttgat agaaagtatg
    16741 ataaggctta tggaagtcaa tttgattatg ctagtgacta tctagccttt ttaaagggta
    16801 aagttggtta tgatgttgat ttgattgatg acggaatgga ttgtaattct tgggaagcta
    16861 gaccttggga taaagatgag aagttacatc caacaaactg ggttgtgagt gagtccattt
    16921 catttttaca aagaagagat ccaacggttc ctttttttct caaaatgtct tttgaaaaac
    16981 cacatgctcc attaaatccc ccaaaatact attttgatat gtatatggaa cgcttgcctc
    17041 aatttctcga tttgcatatc gggaattggg aagtattaga aaagcaaatt ccgtctattt
    17101 atgctttaag agggaaatta aaggaagatg atcaaagaag aatggttgct gcttattttg
    17161 gattgattac tcatatagac catcaaatta gtcgcttttt gactgcccta aaagaatttc
    17221 gtcatgataa agatacgatt atctggtttg tttcggatca tggagatcag cttggtgaac
    17281 attatctgtt tagaaaggga tatccttatc agggaagtat ccacattcct tcgtttattt
    17341 acgatccggc tggattgata gctggtaata ggggaacaat caagcaatta gtgaaaattc
    17401 aagatatttt tccttcactc gtggatttgg caggaggaac tactacagat gaactagatg
    17461 gacggagtgt taagaattta ttatttggac agtacgaggg gtggcgaaca gaatttcatg
    17521 gtgaacatgc tttaggaaaa gattctagtc aatatatctt gacagaccag tggaaattta
    17581 tctggtttcc tgttttaaat cattatcagt tatttgatat gaaaaaagat ccgcatgaaa
    17641 tgaatgattt gtatccaagt gaaaaatacc aacctattgt tcgtcagatg aagaaaaaat
    17701 tagtagactt tcttagatat agagaagaag gttttgttgt ggatgaagag ttagttcctg
    17761 ttgaactttc taaaataacg ccaactctaa cgaaaacggg ggatagtcaa tcatgaaaat
    17821 gaaaaaaatc aagggtggga catttatgat gggaacaaat tctgaagagg gctttttaga
    17881 tgattttgaa ggtcctcaag tcgctgttag tgtaaaggat ttttccattg cggatactcc
    17941 agttacgaat caagaatttg ctcaatttgt gaaagaaaca ggctataaaa cactagctga
    18001 gcgacaagag tggtcttttg tttttatatt gttcgttcca gaggcagaaa gggagggata
    18061 tcctcatcct gctggggctc cgtggtggtt acaagttccg aacgcttgct ggaaacatcc
    18121 ctacggtgag aatagtaatc tagtgggttt agaagaccat ccagtagttc atgttgcttt
    18181 agaagatgca ttggctttct gtaattggag cggaatgtct ttaccgactg aagcacagtg
    18241 ggaatatgct gctagaggag gaagacaatc tgaatatcct tggggagata ctcttttaga
    18301 aggtggatat tatcatgcca atacttggca aggaagattt ccttatgaaa atactgcctt
    18361 agatggattt ataggaacgg ctcctgtcta tgaattttta cctaatgatt ttggtctata
    18421 tcaaatgatt gggaatgttt gggaatggtg tcgcaatccg agatatacct tattagctag
    18481 ttttaatgaa gatgactatg agttgccaaa atatgggata caagatgagg agtatgctat
    18541 cagaggaggt tcctttttat gtcattgttc ttattgtaat cgataccgag tagctgctag
    18601 aaatggatgt atttctactt ccactagtag ccatctaggt tttagatgtt tgaaagaata
    18661 ggatggactt aaaaagtggt tgagattatt tattttctaa tcattataat agctagtgga
    18721 ttgggttcta tatcaggcat gggtggtgga attataatta agccacttat ggatagtttt
    18781 ggataccatt cggtcagtga tattgctttt tattctagtt tctctgtttt tataatggcg
    18841 attatttcaa caaccaaaag attttcacaa agtaaagaga taaaatggag attgattttt
    18901 acggtatcct tttcttctgt tttaggaggg tttctaggtc atcttatttt tcaagttttg
    18961 ttatctcaat tatcagtacg gctagtatcc attgttcaaa tgattttact atttgtgatg
    19021 ctacttgtat catttgtttt aacagatttt aaaaaaactt atcaatttga taagatagga
    19081 ttctatatga tttgtggtct tctattgggc ttgatttcta gttttttagg gattggtggt
    19141 ggtccgctca atgtatcttt attgatggtg tttttttcta tttcgataaa agaagcaacg
    19201 atgtattctc ttgcaattat tttcttttcc cagttgtctc atttggctac aattgttgtg
    19261 gttacaggtt taaatcagta tcatttagca cctgttccag ttatttttct tgcttctata
    19321 tgcggagggg tattggggac ggttgtatca aaagtcttac ctgaaaattg ggtaagatac
    19381 tgttttaaag ggatgttatt ctttgtcatg ggaatgactt tatataattt atttcatatt
    19441 ttgtaaggtc agtgtgaaaa tatttaaata gacttcctgt aaaaactgga tttaacgccc
    19501 tccatttgtc aaattaagct agacagtctt accaactcag aaaatacctg atgaggtatt
    19561 tggtatccaa aggatttagg tgggatgtga tttcttgagg ctgggaaaaa gtctttaaaa
    19621 ttaaaaaatg tataatatca ggtgttggaa aaactttgat actatgcgtt ttattgtggg
    19681 aatatttatt tcattttctc ctgaaattga gtttttaccc agcctaaaat tcttgactga
    19741 cttcgttgaa atctgtctgc tttggcaagt catgacacct taaaagtcta tttgaatgtt
    19801 cgttgagaca ccgctgtctt ggacatccta gattcgcaaa aaagctctca ctatcgtgat
    19861 gattggaaat atctttccat tttgaaaact cttttccaca gtcaaacgta atagatttaa
    19921 agaggttcac tagaaattgt gccagccacc cttggatgga ttctgccata tcctttgcct
    19981 tacgactctt ggtcttcaaa ctgacaatca attgactata cctgttcgct cacaaatagg
    20041 agagaacttt ttacgaaaat ctaatgaata tgccacaaga acattatacc gcaacgtgta
    20101 ctatttttgg ttcattttac tatagaaact attgaagcac ctgtacgaaa atatagtaga
    20161 ttgaaactag aatagtacac ctctacttct aaaacattgt tagaaatcga tttgactgtc
    20221 ccgatcgatt cgtcctgtta ttatttcatt ttactataca ttatgaaaaa ggggagatag
    20281 aaagcaaaca gccttgaaat caatgttgac tgcgattatg aacctgaatc agcccctcaa
    20341 aagggactgt atatgaatga acgttaccag tgtttaaaaa ctaaagaata tcaggcactt
    20401 ttatcttcca agggtagaca aattttcgct aaacgtaaga ttgatatgaa atctgtcttt
    20461 gggcagataa aggtttgttt gggttataag agatgtcatc tgagaggtaa gcgtcaagtg
    20521 agaattgaca tgggattcat actcatggcc aacaacctgc tgaaatataa taagagaaag
    20581 aggcaaaatt aaaaagctag agttccgcaa ttgggaatct ctagcttttt gttttctgag
    20641 aatcatcttg actcagactc ttttgactaa tgttaccaaa aaagataaag taacttttaa
    20701 ccttatttat cgtcgccact caacatgttt ttaacacctt ctacagcacc ttctacagcg
    20761 tctttagcgt cttcggcaac ttcttttact ttagaaacaa ctttttcagc tgctccttct
    20821 ttttccattt tttcatcacc gatggctttc ccaacacctt ctttaataga acctttagct
    20881 tgatttaatt tttcttctac tgacatgata atttctcctg ttatatttat tttatcttag
    20941 tttacacgag tgttttcgtg agatactaca ccatttgctg cacctttaac agcttctaca
    21001 ccttcgctaa ctttttcttg aacagttgag aaaccagatc caagaccaga tttagctttt
    21061 tcgaattgtt ctgaagtgaa ttctcctgtt gattcagcaa cgtcagatac gcgatcttga
    21121 aggcttactg agtctgcttc atgctgttct ttagttttga tgtcgacaac gtttacgttg
    21181 atttcaacaa tttccaagtc agtcatttta gcaacttctg aagatacgat ttctctgatt
    21241 tctgaatata aagctggaac atttttttgg tactcaacaa taacgtttaa gtcaactgca
    21301 acttgtgttt taccaacttc tacgttaaca ccacttgtta cgtcatcgct gttaacgatt
    21361 ttttctttaa gatttgagaa gaaaccacca tcgattccca aaagacctga aacgttttct
    21421 agtgaaagac caatgatttt ttggataact ttatcttcgt aagtaagttc ccctttgatt
    21481 tcgtgagcta caacagtagc atctttcttt tctacgttag tgtttgtgtt tttttcgttt
    21541 gacatatgaa actcctttaa ttaaattagt aatttttatt tttttattta tctatatagt
    21601 ttttttcgat atataatcca gctgcaactc ccagtgctcc taaaatcaat acaaatattg
    21661 ttttgaagaa gccaaaggag acaatcaaac aagccagaaa tacgcctatg agaccagcga
    21721 taattggata tcgatattgt ttaagccatt ccatttttta cttccttact tcacacgact
    21781 aacagtcttt ttgttttgag gttttggttg gtaatttttt actgcaactt caagttttac
    21841 ttgacgctca ataccaaaaa actgcttcaa tccattagtt atttcatttt gaattatttg
    21901 gcatctgtca gcgatgttgt ctgaaggaag aattttacct tctacatgaa cgaaacattt
    21961 attttttcgt aaatttacat gaacagttgg gttcttgatc aatctatgat cactcaccaa
    22021 acttctaaca aaaccttcga ttgccgaatt ctttaatttt aatgtatcgt ttttagtttc
    22081 aagttggatt tccaagtaac gtttaggata aaacatcaca actaaaatgg ataataaaac
    22141 taaagttgat agaaccactg tcccccaaaa gacatatcta gcaagataga attcaactac
    22201 ggagttctgt ctccagctaa gtagatgaat acctagatca ctaacttgat gataatctat
    22261 caaaacagga aggaaaattg tcaagattaa aatacagaaa ataatgaaac atattttctt
    22321 tgcttttgac atatacaatc ctttcgattt aacatttaaa ctatttttcc cgtcattgat
    22381 ttaaaaatag tttgctaact ttactggcga aaagtttact ttctacctgt aaagaatgac
    22441 actacagtaa tcacaatcgc tgcacctaca atagatggga gcaaagccat tccagcgatt
    22501 gatggacccc aactacctaa aagagattgt cctgcatatg ccccgactaa accagcgaat
    22561 acatttgcga tgattcccat agaactactt ttttagtgat tttacctgcc aagagaccaa
    22621 taagacctcc agcaataatt gatcacaaca ctgcctaccc tcctttttta ttttaatatc
    22681 aaaaaatcaa tttctttgat taatactatt atataacttt aatatagaaa agtcaattat
    22741 ttcgattgtt attattatat aattttaaaa aatcaaattc tttaattgtt gctattatat
    22801 aatttttaaa attaaaaata taatctttag acttgaattt gattctgtat tcacgccctc
    22861 aatagacggt aaaataagaa aattgtttat atattgtctc cgtagtgtta ttatacgaaa
    22921 taaaagattt taggaaaagt cgttatatca tgctatacct attctttgtg gtataattgc
    22981 aagaggttta atccgataat ttataaagag agaaagatat tcatgagaga tttattatct
    23041 aaaaaaagtc ataggcaatt agaattatta gaattattat ttgaacataa acgttggttt
    23101 catcgttctg aactagcaga gttattaaat tgtacagaac gtgcagtcaa agatgatcta
    23161 tcccatgtta aatctgcttt tcctgacttg atttttcatt cttctactaa tggtatacgc
    23221 attattaata ccgatgatag tgatattgaa atggtttacc atcatttctt taaacattca
    23281 actcattttt cgattttaga attcatcttc tttaatgaag gttgtcaagc tgagagtatt
    23341 tgtaaagaat tttatatcag ttcatcttcg ctctatcgta ttattagcca aatcaataaa
    23401 gtgattaaaa ggcaatttca atttgaagtc agtctgaccc ctgttcaaat cattggaaat
    23461 gagagagaca ttcgttactt ttttgcacaa tatttttcag aaaaatatta tttcctagaa
    23521 tggccatttg aaaatttttc atcagagcca ctatctcaat tgttagaatt ggtttataag
    23581 gaaacaagct ttccaatgaa tttgtcaacg catagaatgc taaagttgct cctagttacg
    23641 aacctatata gaataaagtt tggtcatttt atggaagtag ataaagattc ttttaacgac
    23701 caaagtttgg attttttaat gcaggcagaa ggaatagaag gtgttgctca gagttttgaa
    23761 tcagaataca atatctcttt agatgaagaa gttgtttgcc aattatttgt gtcttacttt
    23821 caaaaaatgt ttttcataga tgaaagtctc tttatgaaat gcgtaaaaaa ggatagttat
    23881 gttgaaaaat cttaccatct attgagtgat tttattgatc agatttcagt caagtatcag
    23941 attgaaatgg agaataagga taatctgatt tggcatctgc ataataccgc acatctgtat
    24001 cgtcaagagt tgtttactga gtttatttta tttgatcaaa aagggaatac aatcaggaac
    24061 tttcaaaata tttttccaaa atttgtttca gatataaaaa aagagctttc tcactattta
    24121 gagactcttg aggtttgttc tagttcaatg atggtaaatc atctatctta taccttcatc
    24181 actcatacca aacatttggt gattaatttg ctacaaaatc agcctaaact aaaggtttta
    24241 gttatgagta attttgatca gtatcatgca aaattcgttg cagagacact ttcttattac
    24301 tgtagcaata attttgaact ggaagtttgg actgaattag aattatcaaa ggaatcttta
    24361 gaagattcac cttatgatat cattatttct aattttatta ttcctcctat tgaaaataag
    24421 agactcatct attcaaataa tataaacacg gtctcactca tatacttgtt aaatgccatg
    24481 atgtttattc gattagatga gtaaccaaaa ataacaaaaa aacgctattt cgcgtttgaa
    24541 ctgtacctca aaagttagac agaaaaaatc taatttttgg ggtgtttttg ttaagaaaag
    24601 accttcaatc atttaagcaa aatgtacatc tagatttata gccctctaaa actcttgctt
    24661 tttttgtaga agatacgaaa aaagccataa aaccaaggct tttcctgttt tatgtagatg
    24721 tttacacatg aaaattgaag tattttcaaa agctaattgg agtgatttta ttcatgatgg
    24781 gataaatgat ctggccaaaa atgattgaga aatagttgct taaaaagcat ttgcatgata
    24841 ctatatattg aaaggaggtg ataatatggt agaacaaaga aaatcaatta ccatgaaaga
    24901 tgttgcttta gaagcaggag ttagtgttgg aactgtttca cgtgtaatta ataaagaaaa
    24961 aggcattaaa gaagtaactt tgaaaaaagt ggaacaagcg attaaaactt tgaattacat
    25021 tccagattac tatgctagag gaatgaaaaa aaatcgaaca gaaacgattg caatcattgt
    25081 accaagtatc tggcatccct tcttttcaga atttgctatg catgtggaaa atgaagtcta
    25141 taagagaaat aacaaattac tcttatgttc tataaatggt acaaatagag agcaagacta
    25201 tctggagatg ttgcgtcata ataaagttga tggagtggtt gccattacct atagtccaat
    25261 tgaacattac ttgacgtcag gaattccctt tgttagtatt gaccgcacat actcagatat
    25321 tgccattcct tgtgtttcat cagataatga tgcaggtgga agagaagcag ctaaacaatt
    25381 aattagtaaa ggatgtcagc atttagcctt tgtgggcgga cataatacaa ccattaatga
    25441 aaccaaacgt cgtagaattt catttgaaaa gtatgtccaa gagcataaaa tacctcttag
    25501 tatttttgat ttggatgaga cagttactga ctattatgga aagctagaga gtttcttgga
    25561 agaaaattct tctatagatg gaatttttac aatcaacgat tttacagctt tggatgtgat
    25621 tgaaatacta gaaaaaaatg gcaaacgtat tcctcatgat gtccaaatta ttggatacga
    25681 tggaattaaa atggctggag atagagatta tctactttca actattgaac aaccattgga
    25741 agaaatggca aaagaagcgg ttcgtatttt gtttgatata attgatggaa agactgttaa
    25801 tttgcagaca atcctaccag taaaatttgt tgaaggaaaa acaacaaaaa atgaaaataa
    25861 gccttgacag aaagcgctat caatgataga atgaattcag ataaaaagat ttatttttaa
    25921 aacaaaaatg aaacgtttca aaaaaagaaa taaaaagaca gcgccaagcg ctatctttta
    25981 tagaaaaaaa tgaaacgttt caaaaaaagg aggttgctat gaatagcaaa gcgaagcaag
    26041 tttctctttg ggaaagaatc aagaaacaaa aactcttgtt attgatgact gtccccggtt
    26101 tagttttaac ctttatcttt aaatacatcc ctatgtatgg ggttttaatc gcatttaaag
    26161 attacaatcc tttaaaagga attttaggga gtgattggat tggtttttct gagtttacaa
    26221 aattcatatc ctctcccaac tttggtatct tgttagccaa cacattaaaa ttaagtatct
    26281 atggtttatt gcttggcttt ttaccaccaa tcattcttgc gattatgctc aatcaactct
    26341 tgagtgaaaa agtcaaaaaa cgaattcagc tcattttata cgcaccaaac tttatctcag
    26401 tcgttgttat tgtcggtatg attttcctct tcttttcagt gggaggacca atcaacaatt
    26461 ttctttctat gtttggaatg aaggctgact tcttgacaaa tccagacttc tttagaccct
    26521 tatacatctt tagtggtatc tggcaaggaa tgggctgggc ttcaacgctc tacacggcaa
    26581 cattggtaaa tgtagatcca gccttagtag aagcagctcg actggatgga gccaatatct
    26641 tccaacgaat ctggcacatt gatattccag ctcttaagcc tattatggtt atccaatttg
    26701 ttttagctgc aggtggaatt atgaatgtcg gatatgaaaa agcattcttg atgcagacat
    26761 cgttaaattt gccaacttct gaaattatct cgacatatgt ctataaagtt ggtcttgtat
    26821 caggagacta ttcttactca acagcggttg gtttgtttaa tgcagtgatt aacgtagtat
    26881 tgcttgttgc agttaaccaa atcgttaaac gcatgaataa tggtgaagga atttaaggag
    26941 gaaagtatga aaaattcgat tatggataca aaatttgata gacgtatctt actcttaaat
    27001 aaaatcatta ttgtctttat cgttttgatg actttgcttc ctttacttta tatcgtcgta
    27061 gcatccttta tggatcctaa ggttctggtt agtagaggga ttagctttaa tccagccgat
    27121 tggactgtag aaggttacca gcgtgtattc agtgaccaat ctattctaag aggttttatc
    27181 aattctctac tatactcttt tggatttgca gctttaacag tattgctatc tgtgtttaca
    27241 gcttatcctc tttctaagaa agacttggtt ggacgtcgtt ggattaacta cttcttgatt
    27301 gtgactatgt tctttggtgg tggtttagtc ccaacttact tgctcgtaaa agaattggga
    27361 atgctcaata ctccatgggc tatcattgtt ccaggtgctg tcaatgtttg gaatattatt
    27421 cttgctaggg cctatttcca aggattgcct gaagaattag ttgaagctgc tgtcattgat
    27481 ggagcgaatg acttgcagat tttctttaaa atcatgcttc ctcttgcaaa accaattatg
    27541 tttgttctct tcctctatgc ttttgtagga cagtggaact catactttga tgcaatgatt
    27601 tatatcaagg atccaaactt ggaaccattg caacttgtac ttcgtaaaat tctcattcag
    27661 agccaaccag gtcaagacat gattggagca caagcggcta tgaatgaaat gaaacgttta
    27721 gctgaattga ttaaatacgc aactattgtc atttccagct tgccattgat tgttatgtat
    27781 ccattcttcc aaaaatactt tgataaagga attatggctg gttcacttaa aggataaaaa
    27841 aagaaaaaat aaaaggagtt ttctcatgaa attcaaaaca ttctcaaaat cagcagtttt
    27901 gttgacagct agtttagcag tacttgcagc ctgtggctca aaaaatacag cttcaagtcc
    27961 agattataag ttggaaggtg taacattccc gcttcaagaa aagaaaacat tgaagtttat
    28021 gacagccagt tcaccgttat ctcctaaaga cccaaatgaa aagttaattt tgcaacgttt
    28081 ggagaaggaa actggcgttc atattgactg gaccaactac caatccgact ttgcagaaaa
    28141 acgtaacttg gatatttcta gtggtgattt accagatgct atccacaacg acggagcttc
    28201 agatgtggac ttgatgaact gggctaaaaa aggtgttatt attccagttg aaaatttgat
    28261 tgataaatac atgccaaatc ttaagaaaat tttggatgag aaaccagagt acaaggcctt
    28321 gatgacagca cctgatgggc acatttactc attcccatgg attgaagagc ttggagatgg
    28381 taaagagtct attcacagcg tcaacgatat ggcttggatt aacaaagatt ggcttaagaa
    28441 acttggtctt gaaatgccaa aaactactga tgatttgatt aaagtcctag aagctttcaa
    28501 aaacggggat ccaaatggaa atggagaggc tgatgaaatt ccattttcat ttattagtgg
    28561 taacggaaac gaagatttta aattcctatt tgctgcattt ggtatagggg ataacgatga
    28621 tcatttagta gtaggaaatg atggcaaagt tgatttcaca gcagataacg ataactataa
    28681 agaaggtgtc aaatttatcc gtcaattgca agaaaaaggc ttgattgata aagaagcttt
    28741 cgaacatgat tggaatagtt acattgctaa aggtcatgat cagaaatttg gtgtttactt
    28801 tacatgggat aagaataatg ttactggaag taacgaaagt tatgatgttt taccagtact
    28861 tgctggacca agtggtcaaa aacacgtagc tcgtacaaac ggtatgggat ttgcacgtga
    28921 caagatggtt attaccagtg taaacaaaaa cctagaattg acagctaaat ggattgatgc
    28981 acaatacgct ccactccaat ctgtgcaaaa taactgggga acttacggag atgacaaaca
    29041 acaaaacatt tttgaattgg atcaagcgtc aaatagtcta aaacacttac cactaaacgg
    29101 aactgcacca gcagaacttc gtcaaaagac tgaagtagga ggaccactag ctattctaga
    29161 ttcatactat ggtaaagtaa caaccatgcc tgatgatgcc aaatggcgtt tggatcttat
    29221 caaagaatat tatgttcctt acatgagcaa tgacaataac tatccaagag tctttatgac
    29281 acaggaagat ttggacaaga ttgcccatat cgaagcagat atgaatgact atatctaccg
    29341 taaacgtgct gaatggattg taaatggcaa tattgatact gagtgggatg attacaagaa
    29401 agaacttgaa aaatacggac tttctgatta cctcgctatt aaacaaaaat actacgatca
    29461 ataccaagca aacaaaaact agaggttgat tatgggagat aagaaataca cagtagaaaa
    29521 agccaatcgt tttatagcag aaaataaaca tctcgttaat actcaatata agcctgaaga
    29581 acatttttca gctgagattg gttggatcaa tgatccaaat ggatttgtct attttcgtgg
    29641 agaataccat ctcttttatc aattctatcc atatgatagt gtttgggggc ctatgcactg
    29701 gggacatgct aaaagtaagg acttggtgac ttgggagcac ttgccagtgg cacttgctcc
    29761 tgaccaagat tatgaccgaa atggttgttt ctcaggctct gccattgtca aggatgatcg
    29821 cctctggctc atgtacactg gacatatcga agaagaaacc ggtgtccgcc aagtgcaaaa
    29881 tatggcattt tcagatgacg ggattcactt tgaaaagatt gaacaaaatc cagttgcaac
    29941 tggatcagac ttgccagatg agttgattgc tgctgatttt cgtgatccaa aactctttga
    30001 aaaagatgga cgctattact ccgtagtagc tgccaaacac aagaataatg tgggctgtat
    30061 cgttctacta gggtccgata acctagtaga atggcagttc gaatccatct ttttaaaagg
    30121 ggtagaacac caaggcttta tgtgggaatg cccagattac ttcgagttag atgggaaaga
    30181 ttgccttatt atgtcaccca tgcgttatca gcgtgaggga gactcatatc ataacatcaa
    30241 ctcatcgctt ttgttcacgg gtaaggtaga ttggggagaa aaatgtttta tcccagaatc
    30301 agttcaagaa attgatcatg gccaagactt ctatgcgcct caaacattgt tggacgatca
    30361 aaatcgtcgt atcctgattg cttggatgca gacatggggg cgtacccttc caacccatga
    30421 ccaagaacac aaatgggcat gtgccatgac tttacctaga attttaagat tggaagatgg
    30481 caaactaaga caattccctg ttaaaaaagg ccaatatcaa atccaaatag ataaagattg
    30541 tcattaccac ttaggaaatg atatagatta tcttgaattt ggttatgata gtaatgcgca
    30601 gcaagtttac attgatcgta gccatcttat tcaaaaaatt ctaggtgaag aagaacagga
    30661 cactagtcga cggtatgtag atattgaagc taaagaattg gaagttgttc tagataaaaa
    30721 ttccatcgag atttttgtca atcaaggtga agcaagcttg actgcaactt attacttaac
    30781 ggtgccagct gagctatcac gaattgatta aaaattaagt tatttctcct aaagaaaaag
    30841 ttctctttct aaaatagtgg aaagaggact ttttgtgttt tgggtatata agcttagttt
    30901 atggtatttg taaaattggt gttggattat gatttaagct agttttctaa agaatttgaa
    30961 aaaaatttta tttaagcaaa aaaaaccctt ggttccaagg cttttcctgt tgtatttata
    31021 tgccccctac agggatcgaa cctgtgaccc acggattaag agtccgctgc tctgccagct
    31081 gagctaagga ggcaaagaaa aaagctgtat tggtgccgaa acttcacggt ttgtattgaa
    31141 cccgcgcaat taagcaggtg ggcaactcgc tctaactgaa gctgtttccg tgtgagacgg
    31201 cctacatgct gttagaagac ttttgtttcc ctaataatac aaaaaatagt cggtcaacac
    31261 ttaagtgtga agtcgtacac cacagcgttt ctatacttat atgataccac tttttcaaaa
    31321 aaaatcaaga ggaaagtgca attttttgaa aaggatttca gatttttcgc aaacggtcga
    31381 tagcttcctt tctttgagcc aaagcccgtt catatttacc agtatcttct gcttggaaat
    31441 agtgatgatt acgaattttt tctggcagat agtcttgctt gacccaattt ccaggatagt
    31501 tgtgtggata gagatagtct tgggcattcc ccagttcctt gcttccactg tagtgcccat
    31561 cacgtaggtg tcgcggaata ggcaagtgcc ctgatgtttt gaggtcagca agtgccttat
    31621 ccatagctac ataggctgag ttggattttg gagaaagggc caaatcaatc acgacattgg
    31681 caatgagaat gcgggcttct gggaacccaa tcttctgggc agcatccaga gcagtcacgg
    31741 tatgaatctg ggcctcagga ttggctaagc cgatatcttc ataggcgata acagtcaagc
    31801 gacgagcgag actaggcaga tccccagcct caatcaagcg ggcagtatag tggagactgg
    31861 catccacatc tgagccacga atagactttt gcagggcaga tagaacatca tagtgaccgt
    31921 ctccatcctt gtccatagtg atgtagcttc tctgaagact attttccatg atgtctaagg
    31981 tgatgtggcg aatgccctcg tcattctcag gggtagagag aacagccaag tccagtgagt
    32041 taaaggcaga gcgaaggtct ccgtttgtag aggttgcgat gaaatccagc gcatcctcat
    32101 ctagttctat tggaaaatca aaaccacgtt cagggttact tagagctatc tgcagggcct
    32161 ctttgacgtc ttggttagac agaggttcca actcgaaaat ttgaacacgg ctacgaatgg
    32221 caggagtgac agagaagaaa ggattttcag tcgttgctcc aatcatgatg accagtccac
    32281 tttccaagag aggcaagagg aagtcttgct tggtcttatc tagtcgatga atttcgtcta
    32341 gcaataggac gagaccacca gaaaatttag cttcttccga gatttcttgc agtcgctttt
    32401 tactatcaac tgtcgcattg aaagttcgaa aggcatactt ggtcgttcca gcgatggcag
    32461 aggcaatact ggttttgccg attcccggag ggccatatag aatcatggag gacaggcggt
    32521 tggcttccac catgcggcgg atgatttttc caggtccgac cagatgctcc tgaccgatga
    32581 cctggtcgat ggttttaggg cgcatgcgaa gcgcgagatt gtctggcata gcagtccttt
    32641 ctaacgtgga tttgagtaga atgactcatc tactatatct tactatgtcc attctagcct
    32701 attagttgtg atatttcaat ggttttgtaa taatcaggga gtgagataga atctttttta
    32761 ctatttaatt taatgaggag aggtatcagc atgagagatt tgattttcct taaagctcaa
    32821 aataggatag ttgagcctaa agaagtggaa gtgctgactt gagaaataat attgctgaat
    32881 gtcattgtat gaactataat gaaaagagag gtgataagca atggaaatga tggaccaagt
    32941 caggaaattt tgatttttac aaaacaaatt cgtcattgga ttcttagtga tcaagttatt
    33001 tcagggaaaa gaaaactttt cttccgggaa gatactccaa aagagatttt agatttgtac
    33061 gaaaacatta aatctaaact tgattttgct taccaagaag tttattctaa taatgggttg
    33121 aaaaaatatg aaaaatagaa taattgatgt ttttgaagta gtaaatcgca tccttgtaat
    33181 aactgttgaa aatcctgatt tcgaagactt gagagttaat catactcaat gaaaatcaaa
    33241 gagcaaacta ggaaactagc cgcaggctgt acttgagtac ggtaaggcga cgttgacgtg
    33301 gtttgaattt gattttcgaa gagtatcagt ttgtaaaaat tggagataaa aaatatagag
    33361 tacgcagtgt tcctatgatt cattcaactc cgcctcaatc tgtcttagat cgagatactt
    33421 ttacaattga ctatacagat gatgaattgc tcgataaaga agcagttttt actactcgtt
    33481 agaaaaatag gtatgaataa attggcttcc cttagaacca catctagggg cttttatgta
    33541 taatagaata ataagtatca ctcctttaca atacgtgtaa cacgtgttat aatggtgttg
    33601 tcaggaggta aaaacgctat gcctatgaca caaaaagaga tggttaaact ccttacggcc
    33661 catggttgga taaagactag aggcggtaaa ggttcccata ttaaaatgga gaagcaaggg
    33721 gaaagaccta tcacaatccc tcatggtgag ctgaataagt acactgaaag agggataaga
    33781 aagcaagctg ggttgtaaaa ttcagcccct gctttttgat atggaggtag tgaaatgtta
    33841 gttacgtatc cagctttatt ttattatgac gacacagacg gaacagaagc gacttatttt
    33901 gtccatttcc cagattttga atactcagcc acacaaggag aggggatttc tgaggctttg
    33961 gctatggggt cggagtggct agggataact gttgcagatt tgatagagag tgatggagag
    34021 ttacctcagc catcagatat caatagcttg tctttaattg ataatgatcc ttttaaagat
    34081 gatgaagatt tcgtgtcaac ctatgacctt gataaatctt ttatttctat ggtatctgtt
    34141 gatgtatcag aatacctagg aagtcaggaa cccattaaaa agactttgac cataccaaaa
    34201 tgggcagata agttgggacg agaaatggga cttaactttt ctcagacttt gacagatgct
    34261 attgcagata agaaagttca agcctaagat taagcgtggt atcgtggtaa tagtcaataa
    34321 aaaagcacgt ccacttgtgc tagttactcg cctattgatt ttatagattt attagccctt
    34381 tcgcgggctt tttatattga tttttatgag aaatgaagaa atcagacttt taagaaaatc
    34441 actgatacca agggtttaaa tgaggtaata tggtataatt aggacataaa ataattttgt
    34501 ggtaagatgg tagtatctat tttagcatat ttccgagcaa tcggggcgat taaagagtcg
    34561 catagaaaga ggacaaaatg gcaacatacg gatttttaga tattttagag gaagagttgg
    34621 acaagaactt tccctttgac tttgagatta gttgggacaa gcgtaaccac gcggtagaag
    34681 tgagttttct attggaagcg caaaatgctg caggtgtgga gatggtggat gaagacggag
    34741 aggtttcatc agatgacatt ctttttgagg aagcggtcct tttctacaat cctgccaagt
    34801 caaccgtcaa tgcagaagac tatttaacgg ttatccctta tctacctaaa aaaggatttt
    34861 ctcgcgagtt tttagcttat tttgcccttt tcctcaaaga tactgcggag gttgggctag
    34921 ataccctcat ggactttttg gaagatccag aagcagagga atttgttatg gaatggaatc
    34981 aagaagtctt tgaagaagga aaagttggct tggaagaggg agaattttac ccttatccaa
    35041 aatactagga gttggttgga ggtgttatga agaaaattgg gacttatttg gtttatgtgc
    35101 tagctgttgt ctttattatg ctagcttttg cttgtggaac aatcgcattt gcagagttgg
    35161 ggtattccgc agttttagcc tttacttttg gttatgcctt cgctcttcta agcatgtatt
    35221 taatctttat tcttcatgag ctgggtcatg ctttttgtgg ctacttgaca ggctatcagc
    35281 tggtggcttt tggattagga cattttattt tgacaaaaaa gtcaggcagg tttcatctta
    35341 gtagaacagc cattctgaaa aatgttggtg ctcaatacat tggtttaaaa gaggatgaaa
    35401 gcgatcaaag aatcatcctg atgctttcag gaggcttgat ggttcatctc ggcttgatat
    35461 tattggcgat agtgtttgga tttttgacaa gaagctggta ttttgcaggg acttggattt
    35521 ttcttaattt atctttcttc ctaaataaca ttttaccagt cggcatcacc gatggagcaa
    35581 aaatttggga attactacaa caccctgaaa ataccaaata cgcctacctg gtattgagac
    35641 attctgctca aactttgctg gctcctcaag aatatgattt gaaagacttt gttcaagcag
    35701 ttcctgagga tgcacgaggg agttttgcgg atggtgtatt aggtatgcaa ggtgaagttt
    35761 ccattttgga agggaaggaa gaagtagcta aacagcagtt tcaagcatta cttgagagga
    35821 caactactcc gatgatgcag acagttgccc aactatccct acttcatatc gccttgttgg
    35881 aaggagattt tgagcaagcg gagcaatatg ctagtattcg acaggtcaaa tcgtttctgt
    35941 ctctcaaact gtccaatctt caagtgatac aagcctggta tcaattaaag gtgaagaaag
    36001 atgtagatcg aactcgtaag gctctcaaga ttgctagaca gaaaatggat agtagtcgaa
    36061 tgttgaggga cgagaaacgc tattatgaaa acttgttagc agagctggaa aaggaattag
    36121 ccgaaggagt ctaatatgga aatagagatt tctgatttca caggttgcaa gattgctttg
    36181 ttttgtgggg ataagctttt gactatctta cgtgatgata aggcaagtat tccctgggcc
    36241 aatatgtggg aactgccagg tggtggaaag agtgtcaggg aaaatctgat atagttttgt
    36301 attaaaagaa tatcctttca aggttattct tttttaattt tgtcccaaaa taattgactt
    36361 tgtcccgaaa atatagtatt gtttgtataa ggaggaatga atcatgaaaa aatcagatgt
    36421 tcttgattta ataaagtatc attatgaggg cagagagaca gaatttagaa atcaatccat
    36481 agcaattgct cggaacttca ataaacatgg tgatacacaa atagcacaat atattatggg
    36541 gttaatgtct caaagtgata gattcatgcc acaaatagaa aatcctagtg agtatttgac
    36601 tcctgctaaa ctagatattg gtcctctccc attgcctctt tcaattatga atgatttaaa
    36661 aggaataata aacgctgtca atcatcatat tggaataaat aaatttttat ttgtgggatc
    36721 tccaggtact ggaaaaactg aaagtgtaaa gcaggtggct agacttattg gcaaggaatt
    36781 acttgtagta gatttcagtc acttagtaga tagtaagcta gggcaaacag tcaaaaactt
    36841 agcaactctt tttaatgaaa tcaataatct tcctttcaag caaaattata tcatcctatt
    36901 tgatgaaatt gattccatag tgttggatag ggttaatcag aatgatttaa gagaaatggg
    36961 acgagtgact tctgcctttt taaaggagtt ggataggctg tcaccagaaa ttgtattaat
    37021 tgcaacaaca aatctttttg aaaatcttga taaagcagtt acgagaagat tcgatgccat
    37081 aattgacttt gaccgttata ctgatgaaga taaagtagaa gtagctacta ttattttaaa
    37141 tgagttatta aagcaattta agaatgtagc tagagattta aaattattta aaaaaattat
    37201 caatagtgcc aatgtaatac cgaatcctgg tgatttgaga aactcaataa gaacgtcatt
    37261 ggcatttagt gacccatcag atccgcatga ttaccaaaaa cgcctgctaa gaagtttgca
    37321 taatggtaga aatttatcta tttctaaatt atcgaagcta ggttttactg ttagagaaat
    37381 tgagattttg acaggtattt ctaagagtag tgtatcacga gagttaagcg aggattaaaa
    37441 tgaatacatt attgacatta cgaggtaaaa gttttacaca aaaatcaaga aataatggta
    37501 tgggaccaat aactataccc aaaaaaacaa taattacatt ggaacatttg aagtatcttc
    37561 atttttcatt agaagagacg aaaacatatt gggagaaaaa taacattatc gatggtattt
    37621 tgatcagtat ttactataat cgaattgttg ccaaaagtaa ccgtattaat ggctatttca
    37681 atgtaggagg agggaatcca ttccctaatg atactattgt gggagcaaaa ttcaatgatg
    37741 agaagacaaa acatattgtt actcattata tttctagaga tgctcttaat aaaacaataa
    37801 ccgttttaag taagataatt gaagtttttg aggaacattt tgatagagcg ataacatgtg
    37861 agatgttttc tgattcatca acgtttgctt ctataaattt ttcggagtat ggtatttcaa
    37921 aaagcaaatt tcaacagtat ttgagagatt cttgctttat tgagaatttt ggagttgagc
    37981 acactactgt ttctgatatt caaaattcta tcgtgacatt ttatgatgtc catacagaca
    38041 tttttagatt actgaataaa ttaaatattg atatttcaga agctaatatc atgaatcaga
    38101 ccacggtctt gcttgatgaa aaaaatattg aattgctact atcaaaagcg ccctatcttg
    38161 tctccatgat tgtggaagat ttttctaagt tatctgtaga tgatttttct ttagataata
    38221 atgatttaaa aataaatctt ccatctccaa tgaacgaacc agttgttggc gttattgata
    38281 ccttatttga caaaagagtt tactttaatg agtgggtaga atatcatgat tttgtctcac
    38341 ctgatattag caaagatagc caagattaca agcatgggac agctgtgact tctttaatcg
    38401 ttgatggagc caatttgaac cctaatctgg atgatggttg tggaaatttt agagtgaggc
    38461 attttggtgt gtcactacaa agtggattta attcatttac aatcataaaa cagataaaag
    38521 agattgtttc tcagaatgct gatattaagg tttggaatct ttctttaggg tccaatgatg
    38581 aaataagaga aaattttatc tctgcagagg gagcattatt agacgaaatt caatttgaga
    38641 atgatgtaat ttttatcata gctgggacaa atgcttcagt aataaatggg aaacgcaaaa
    38701 gaataggtgc tcctgctgat tcacttaatt ctataatagt aaattctgtt gattttaata
    38761 atcaatcagt gtcatattca cgagagggta ttgttttatc attctttgtt aaaccagatg
    38821 tgtcatatta tggaggagga aatggtgact ttataaatgt ttgtgaacct ctaggattag
    38881 gtcgtgtagc tggaacaagt tttgcggcac cttttattgc aaggaaaatg gcctatctca
    38941 ttcatattat gggacttagc cgagaagaag cgaaggcttt gctaattgat gcagcaatac
    39001 cctggaatga taagaaaaca ttcactgatt tatctttgat aggaaatggt attgttccaa
    39061 taaaaatgga tgatatttta tctacacctg atgatgaaat taaatttatt gtgtcagata
    39121 tctctagggc ttatgatacc tataattatg attttccagt acctatttct agtgagagtt
    39181 atccatatgt agctaaagca acaatgtgtt atttccctaa ctgctcaaga aaacaagggg
    39241 ttgattatac aaatactgag atgcagctta cttttggtag gttaaagtca gacggtatca
    39301 aatcgataaa taaagacaac cagcatgccg aagatacccc aggatatgtg agagaaaacg
    39361 ctgctagaaa tatatttagg aaatgggata atgtcaagca tattggagag tcgtttactt
    39421 ctcgaaagag agctaaagca attttaaatc catctaatcc tcagtggggg atgagtatta
    39481 aaacaatcga acgtttgaaa agcggtgatg gacaaggagt acgttttggt gtagtagtta
    39541 cattaaaaga attaaatggg gttaatcgta ttgaagattt tattcagcaa gctgaattac
    39601 gcggttggct agttaataga ttgcaagtag aagctcaagt agacctcttt aatagtttaa
    39661 atgaagaaat tgaatttgaa tagtggatat tttatcctta ttttagatct taggaacaca
    39721 agagggcaaa gatatgaata ttacttatat agttggaaat ggtttggatt tgcaatatgg
    39781 tttgaaaaca agatataggg atttctacga attccaaaat aaggtttata taagtaggac
    39841 agagaacgag gaaaaatatt caaatttcat ttatgagtct ctattttctg ataaagtaaa
    39901 tgattatgaa aattggtcag attttgaatt gtcaattggt aaattgacaa aagataacga
    39961 cctcatctcc tcaagtatag aaataaagga gaaatttata gatgatttta gtgaagtagt
    40021 tgatgattta agagaatatc tacgcataca acaagaaaaa aatcttgaga aaggtaatgc
    40081 aattgacttt ataagcactc tagatgatat gaggacctct ctccctgtaa ttaatcaacc
    40141 tgctatcgat aaaaaataca atgagaatcc acatcaggac gacattgtaa atatagtaac
    40201 acttaattat acacatgtaa tcgataaatt atataatggg tcagcaaaat cattcaggaa
    40261 tcaattacga gctaatcttt ataattttta tattgagcca ccaattcatg cacatgggac
    40321 ggtggatgtc tgcacggttt taggggttag tgatgaaata caaatttcaa atagtttaga
    40381 agaacaaaag gaatccctaa taaaaagttt agttttaaaa aactatagag aaaatatgga
    40441 tgtaaagaat agtgatatta taaaaaattc tgacatcatt attctctatg gggtttcttt
    40501 gggagaaact gataggtata tctggagtca gattgctgaa cgatcaatca gcggttctgt
    40561 tcctgtcata atttaccact atgtacctca ttttgatccg gggaatccca ttagagcaaa
    40621 acgcctatat agaaatgtag aagataaatt catacagaat agtggtatag acttagagtt
    40681 agagaaaaaa ttaagagaca accttattgt cgttattggt aagactattt ttgatctaat
    40741 agagaggtaa ttttaaaaaa caaaatggaa acatggcaag agttaaaagt tacagtgaag
    40801 cgtgagggag aggagttggt ttccaatctc ttgattgagc tgggagcgca aggtgttgcg
    40861 attgaagaca gtatggacta tgtgggaaat gttgaccgct ttggcgagat tttcccagag
    40921 gtggagcagc aagaagaaat cgtagtgaca gcctactacc ctgacacggt ggatgtgaca
    40981 gtggttgagg cggacttgca ggctcgctta gcagaattga cggattttat ggatttggga
    41041 gagttaaaaa taggtacgac tgtcttggct gaggaagact gggcagataa ctggaagaaa
    41101 tactatgagc cagctcgtat cactcatgat ttgaccatcg ttccctcttg gacagactat
    41161 gaggcgactg cgggggaaat gattatcaag ctggatcctg gcatggcttt tggtactgga
    41221 acccacccaa ctaccaagat gagccttttt gccttggaac aggttcttcg tggtggcgaa
    41281 acggtgctag atgtggggac tggttcaggg gttctctcta ttgccagctc gcttctgggg
    41341 gctaaagaaa ttttcgccta tgacctggat gatgtggcgg ttcgtgtggc tcaggaaaat
    41401 attgagctca accctggcat ggaaaatatc catgtagcgg ctggagattt gcttaagggt
    41461 gtggaaattg aggcagatgt gattgtggct aatatcttgg cggatatcct cattcatcta
    41521 atagacgatg cctatcgttt ggtcaaggac gaaggctacc tgatcatgag tggcattatc
    41581 aaggataagt gggacatggt gcgcgagtcg gctgaatcag ctggattttt cctcgaaact
    41641 cacatggttc aaggggaatg gaatacctgt gtctttaaga aaaccaagga tatctctggt
    41701 gtgattggag gctagcatgc agcagtattt tgtaaaaggc agtgctatct ctcctgtcac
    41761 tatcgaggac aaggaaacca gtaagcatat gtttcaggtt atgcgcttga aagaagagga
    41821 tgaggttacc ttagtctttg atgatggcat caagcgcttg gcgcgcgtgc tggatatgga
    41881 aaatcgtcag tttgagttgg tccaagaatt aaatgacaat gtggaactac cagtccaagt
    41941 gaccatcgca tctggatttc ccaagggaga caagctggag ttcatcgctc aaaaagtaac
    42001 cgaactgggg gctagccaaa tctgggcctt tcctgcagac tggtcagttg ccaagtggga
    42061 tggcaagaaa ttgggtaaaa aagttgaaaa actagaaaaa attgcccttg gagcagccga
    42121 gcaaagcaag cgtaatattg taccaagtat taaacttttc gagaaaaaag cagattttct
    42181 agctcagctg gaccagtttg actctatcat agtagcctat gaagaatcag ctaaagaagg
    42241 agaagccgct gcgcttctgc aagcagtcac tggtcttgaa acaggagcca aactgctctt
    42301 tatctttggt ccagaaggcg gtctctcacc tgcagaaatc gaaagttttg aagctaaagg
    42361 agccgttttg gcaggcctag gtcctcgtat tttgcgagca gaaacagcac cgctttacgc
    42421 tttatcagcc cttagtgttt tagtagaatt agagaaataa gaggaagaaa atggaacaaa
    42481 aacaccgttc agaatttcca gagaaggaac tctgggactt aacagcccta taccaagacc
    42541 gtgaggattt cttgcgtgca atcgagaaag ctcgcgaaga catcaaccag tttagccgtg
    42601 attacaaggg caatcttcac acttttgagg atttcgagaa ggcctttgcg gaattggaac
    42661 agatctacat tcagatgagc catattggca actatggttt tatgcctcag acgacggact
    42721 atagcaatga cgaatttgcc aatattgccc aagctgggat ggaatttgaa acagatgcca
    42781 gcgtagcctt gaccttcttt gacgatgcct tggtggcagc agatgaggaa gtcttggacc
    42841 gtttgggtaa attgccacat ttaacagctg ccattcgtca ggctaaaatc aaaaaagccc
    42901 actacttagg ggcagatgtg gagaaggcct tgacaaatct cggtgaagtt ttctacagtc
    42961 cgcaggacat ttatactaag atgcgagctg gggattttga aatggctgac tttgaagccc
    43021 atggcaagac ctacaaaaac agctttgtga cctatgagaa tttctaccaa aaccatgagg
    43081 atgctgaggt tcgtgagaaa tccttccgtt ccttctcaga gggacttcgt aagcaccaaa
    43141 atacggctgc agcagcctat ctggctcagg tcaagtctga aaaactcttg gctgatatga
    43201 agggatacga ctctgtcttt gactatcttc tagctgaaca agaagtggac cgtgtcatgt
    43261 ttgaccgcca gattgacctc atcatgaagg actttgcacc agtcgctcag agatacctca
    43321 agcatgttgc caaggtaaat ggtcttgaaa agatgacctt tgcagactgg aaattggact
    43381 tggacagcgc cctgaatcct gaagtgacta ttgacgatgc ctatgatttg gtcatgaagt
    43441 cggtagaacc tttggggcaa gaatattgtc aggaagttgc tcgttaccaa gaagagcgct
    43501 gggtggactt tgctgctaac agtggcaagg attccggtgg ttatgcggcg gacccatatc
    43561 gcgtacaccc ttatgtactc atgagctgga caggccgttt gagcgatgtc tataccttga
    43621 ttcatgaaat cgggcattct ggtcaattca tcttttcaga caatcatcaa agttacttca
    43681 atgcccatat gtcgacctac tatgttgaag caccgtcaac cttcaatgaa ttgctactca
    43741 gtgattactt ggagaaccag tctaatgacc cacgtcaaaa acgcttcgct ctggctcatc
    43801 gcttgacaga cacctacttc cataacttta tcacccacct cttggaagcc gccttccagc
    43861 gtaaggtgta tacattgatt gaagaagggg agacctttgg agcaagcaag ctcaacagca
    43921 ttatgaagga agttttgacg gatttctggg gagatgctat tgaaattgac gatgatgcaa
    43981 ctctgacttg gatgcgccaa gctcactact atatgggctt gtatagttac acttactcag
    44041 caggactagt tatctcgact gctggttacc ttcatcttaa aaactcagga actggagctg
    44101 aagactggct caatctcctc aaatcaggtg gtagcaagac accacttgag tcagccatga
    44161 ttatcggtgc tgatatttca acagacaaac cactccgtga taccatccaa ttcttgtctg
    44221 acacagttga ccagattatc gcctacagtg ctgagttggg agagtagggg aatagaaaaa
    44281 ttttttagaa tgatgtttat gggcttgtta agcgtcattc tatttgtctt agttgtagct
    44341 attttcaaaa atgtcgttga aggtcgtgat gttaaatttg aacttactgt agaactggta
    44401 ttgatagtta tttacgtcat tttatatcag attgtattta attgagaatt caatattgag
    44461 tcatttaaga ctgccttttt gaacagttgc tagaaatgta gaggtattga ccatgtatca
    44521 agatttacta agaaaaatag cagaagaaaa accaaattat aaccaggaag aaatccagtg
    44581 gttgtttgat catttgggaa atccttctcc agaaattcgt gatgaccttt caaatcaagg
    44641 cttgcactat ctttcaaaag aaaaggatac aacaggtttc tcaagtcagt atggttgggt
    44701 tcattctttt gcacatggag ccgatttact gacagaggtg gtttgtcatc cagactttcc
    44761 taaaaacaga gttcatgaag tatttgatat acttggccaa ctatttaaaa gaatgtcgat
    44821 tctttttaca gatgatgagg attggcgttt agcaagagtg atctatgaac ctattttaca
    44881 agggaagttg gagcaagaac aagtagcttc ttggataaaa actgttgatt ttccgataga
    44941 agaaagggag gatttttaca aattttccaa ctttagatcc tgtctggtgg aagtctatgt
    45001 ccaacttgac cagagaaata gtttacaaga tgagttgaaa gaagctatcc agtcttttca
    45061 atactaggga taagttaata gttctatgat tttcaaaaaa tttccaatca attttcttga
    45121 aaccctttcc tacttttgat agactagtat ctagtttttg aaaaaaggag aaagaaaatg
    45181 aaaaaatttg ttgctgagtt aatcggtacg ttcatgcttg tattcgtcgg gacaggagct
    45241 gttgtttttg gaaatggtct tgatggcctt ggtcaccttg gaatcgcctt tgcctttggt
    45301 ttggcaatcg tggtggcagc ctactcaatc ggaactgttt caggtgctca cttgaatccg
    45361 gctgtttcga ttgctatgtt tgtaaacaaa cgtttgtcat cttcagaact tgtaaactac
    45421 atccttggtc aggttgttgg agctttcatc gcttctggcg ctgtcttctt cctcttggct
    45481 aactcaggta tgtcaactgc tagtcttggt gaaaatgcct tggcaaacgg tgtcactgtc
    45541 tttggtggtt tcttgtttga agtcatcgca actttcttgt ttgtattggt tatcatgact
    45601 gtgacttcag aaagcaaggg caatggcgcg attgctggtt tggtaatcgg tttgtcattg
    45661 atggcgatga ttcttgtcgg attgaagatt actggacttt cagtaaaccc agctcgtagc
    45721 ttggcaccag ctgtcttggt aggcggcgca gcccttcaac aagtttggat tttcatcctt
    45781 gcaccaatcg ctggtggagt tcttgcagcc cttgttgcaa aaaatttcct tggaacagaa
    45841 gaataattga aactcaaaaa gccttgctcc tcatcttgag gaacagggct ttttcgtat
//
LOCUS       DAVDWT010000016        43822 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVDWT010000016 DAVDWT010000000
VERSION     DAVDWT010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.016"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(98..2113)
                     /gene="recG"
                     /locus_tag="VUW50_001015"
     CDS             complement(98..2113)
                     /gene="recG"
                     /locus_tag="VUW50_001015"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET7982927.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFILAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(2132..3235)
                     /locus_tag="VUW50_001016"
     CDS             complement(2132..3235)
                     /locus_tag="VUW50_001016"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET7982928.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKWAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREVSEVEQAQDLLQKHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(3225..3593)
                     /locus_tag="VUW50_001017"
     CDS             complement(3225..3593)
                     /locus_tag="VUW50_001017"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET7982929.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(3632..4663)
                     /locus_tag="VUW50_001018"
     CDS             complement(3632..4663)
                     /locus_tag="VUW50_001018"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET7982930.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYATLTK"
     gene            complement(4665..5696)
                     /locus_tag="VUW50_001019"
     CDS             complement(4665..5696)
                     /locus_tag="VUW50_001019"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET7982931.1"
                     /translation="MVFAAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(5777..8290)
                     /gene="secA"
                     /locus_tag="VUW50_001020"
     CDS             complement(5777..8290)
                     /gene="secA"
                     /locus_tag="VUW50_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET7982932.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(8436..8723)
                     /locus_tag="VUW50_001021"
     CDS             complement(8436..8723)
                     /locus_tag="VUW50_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982933.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(8734..9384)
                     /locus_tag="VUW50_001022"
     CDS             complement(8734..9384)
                     /locus_tag="VUW50_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7982934.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKVLAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            complement(9388..10029)
                     /locus_tag="VUW50_001023"
     CDS             complement(9388..10029)
                     /locus_tag="VUW50_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982935.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSIILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFLLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLILSRLEEKFKKG"
     gene            complement(10022..10813)
                     /locus_tag="VUW50_001024"
     CDS             complement(10022..10813)
                     /locus_tag="VUW50_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982936.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLTIAIITYFLGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAIAYLMFLWLGSILLYST
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLKKYEDNV"
     gene            complement(10998..11333)
                     /locus_tag="VUW50_001025"
     CDS             complement(10998..11333)
                     /locus_tag="VUW50_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982937.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMIRLRRE
                     LGKSKGGRH"
     gene            complement(11474..11809)
                     /locus_tag="VUW50_001026"
     CDS             complement(11474..11809)
                     /locus_tag="VUW50_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982938.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTALRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(11900..13210)
                     /gene="der"
                     /locus_tag="VUW50_001027"
     CDS             complement(11900..13210)
                     /gene="der"
                     /locus_tag="VUW50_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET7982939.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(13224..13937)
                     /locus_tag="VUW50_001028"
     CDS             complement(13224..13937)
                     /locus_tag="VUW50_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HET7982940.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(13934..14830)
                     /gene="dnaI"
                     /locus_tag="VUW50_001029"
     CDS             complement(13934..14830)
                     /gene="dnaI"
                     /locus_tag="VUW50_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HET7982941.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPKEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(14831..16000)
                     /locus_tag="VUW50_001030"
     CDS             complement(14831..16000)
                     /locus_tag="VUW50_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HET7982942.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLAFHAPLSSQD
                     FLGHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRDR
                     GQKAKTQKQNQTAPEKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(16001..16474)
                     /gene="nrdR"
                     /locus_tag="VUW50_001031"
     CDS             complement(16001..16474)
                     /gene="nrdR"
                     /locus_tag="VUW50_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HET7982943.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            16614..16979
                     /locus_tag="VUW50_001032"
     CDS             16614..16979
                     /locus_tag="VUW50_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7982944.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            16984..17679
                     /locus_tag="VUW50_001033"
     CDS             16984..17679
                     /locus_tag="VUW50_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982945.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            17696..18463
                     /locus_tag="VUW50_001034"
     CDS             17696..18463
                     /locus_tag="VUW50_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982946.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(18816..20015)
                     /locus_tag="VUW50_001035"
     CDS             complement(18816..20015)
                     /locus_tag="VUW50_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7982947.1"
                     /translation="MRNMWVVIKETYLRHVKSWSFFFMVISPFLFLGISVGIGHIQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(20012..20905)
                     /locus_tag="VUW50_001036"
     CDS             complement(20012..20905)
                     /locus_tag="VUW50_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982948.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            21022..21102
                     /locus_tag="VUW50_001037"
                     /pseudo
     CDS             21022..21102
                     /locus_tag="VUW50_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            21587..21800
                     /locus_tag="VUW50_001038"
                     /pseudo
     CDS             21587..21800
                     /locus_tag="VUW50_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            22215..22832
                     /locus_tag="VUW50_001039"
     CDS             22215..22832
                     /locus_tag="VUW50_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET7982949.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(22875..23762)
                     /locus_tag="VUW50_001040"
     CDS             complement(22875..23762)
                     /locus_tag="VUW50_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET7982950.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIEFNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(23911..25848)
                     /locus_tag="VUW50_001041"
     CDS             complement(23911..25848)
                     /locus_tag="VUW50_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HET7982951.1"
                     /translation="MDYSKVAAEVIEAVGKDNLVAAAHCATRLRLVLKDEAKVNQAAL
                     DNNADVKGTFSTNGQYQIIIGPGDVNFVYAEIIKKTGLKEVSTDDLKEIANKDKKFNP
                     LMDLIKLLSDIFVPIIPALVAGGLLMALRNFLTSPDLFGPQSIEDMYPAIKGFSAMIQ
                     LMSAAPFMFLPVLVGISAAKRFGANQFLGAAIGMIMTTPDLGGKEAFWDILGFHVTQT
                     NYAYQVIPVLVAVWLLANLEKFFHKKLPSAVDFTFTPLLSVMITGFLTFTVIGPVMLV
                     VSDAITNAIVWLYNTTGAFGMGLFGGTYSLIVMTGLHQSFPAIETQLLSAYNNNGTGF
                     GDYIFVVASMANVAQGAATLAVYFLTKNAKTKGLSSSAAVSAFLGITEPALFGVNLKY
                     KFPFFCALAGSAIGAFVAGLTHVIAVSLGAAGFIGFLSIKAGSIPMYIIAEIMSFVAA
                     FAFTYFYGKTKAASVFADEAATATAETVTEPTVEAPVVEETDTLQNETLVTPIVGDVV
                     ALADVNDPVFSSGAMGQGIAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLI
                     HVGIDTVSMNGDGFEAKVAQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYA
                     SVAPVATGSVAKGDAVIEVKI"
     gene            26224..27678
                     /locus_tag="VUW50_001042"
     CDS             26224..27678
                     /locus_tag="VUW50_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET7982952.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            27659..28624
                     /locus_tag="VUW50_001043"
     CDS             27659..28624
                     /locus_tag="VUW50_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7982953.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(28659..29933)
                     /locus_tag="VUW50_001044"
     CDS             complement(28659..29933)
                     /locus_tag="VUW50_001044"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HET7982954.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(29933..31105)
                     /locus_tag="VUW50_001045"
     CDS             complement(29933..31105)
                     /locus_tag="VUW50_001045"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HET7982955.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(31236..31484)
                     /locus_tag="VUW50_001046"
                     /pseudo
     CDS             complement(31236..31484)
                     /locus_tag="VUW50_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(31597..32178)
                     /locus_tag="VUW50_001047"
                     /pseudo
     CDS             complement(31597..32178)
                     /locus_tag="VUW50_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(32240..32689)
                     /locus_tag="VUW50_001048"
     CDS             complement(32240..32689)
                     /locus_tag="VUW50_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET7982956.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(32691..33449)
                     /locus_tag="VUW50_001049"
     CDS             complement(32691..33449)
                     /locus_tag="VUW50_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET7982957.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(33564..35543)
                     /gene="pknB"
                     /locus_tag="VUW50_001050"
     CDS             complement(33564..35543)
                     /gene="pknB"
                     /locus_tag="VUW50_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HET7982958.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(35540..36280)
                     /locus_tag="VUW50_001051"
     CDS             complement(35540..36280)
                     /locus_tag="VUW50_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET7982959.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(36295..37608)
                     /gene="rsmB"
                     /locus_tag="VUW50_001052"
     CDS             complement(36295..37608)
                     /gene="rsmB"
                     /locus_tag="VUW50_001052"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET7982960.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDWVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(37601..38536)
                     /locus_tag="VUW50_001053"
     CDS             complement(37601..38536)
                     /locus_tag="VUW50_001053"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET7982961.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(38549..40945)
                     /locus_tag="VUW50_001054"
     CDS             complement(38549..40945)
                     /locus_tag="VUW50_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET7982962.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEVAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(41011..41325)
                     /locus_tag="VUW50_001055"
     CDS             complement(41011..41325)
                     /locus_tag="VUW50_001055"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET7982963.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(41350..41976)
                     /gene="gmk"
                     /locus_tag="VUW50_001056"
     CDS             complement(41350..41976)
                     /gene="gmk"
                     /locus_tag="VUW50_001056"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET7982964.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(42106..43719)
                     /locus_tag="VUW50_001057"
     CDS             complement(42106..43719)
                     /locus_tag="VUW50_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET7982965.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTIEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
BASE COUNT        12520 a        10001 c         8373 g        12928 t
ORIGIN      
        1 catctacaac ctcaaaacag tgttttgagc aacctgcggt tagcttccta gtttgctctt
       61 tgattttcat tgagtataag ttttccttag agaaagctta atccagatgt tctttctttt
      121 ccagatgaag ggcaatcatg cgccactctg gatcttcttg ccaagcttct atagaactaa
      181 tgtagctagc aacctttctt gcttcttcta aaatcggaaa atcttcgata atatcagcca
      241 cttggaactc tggaagtcct gactgtctgg ttccaaaaat ctcaccagaa ccacgcattt
      301 tcaaatcttc ctccgcaagg ataaatccat tggtcgtttc tgtcatgatg cgcatgcggt
      361 ctttcccaga atccgtcttg ggattggcaa cgagaacagc gtaggactgc ttgtcccccc
      421 gaccgacacg acctctaagc tggtgaagtt gactgagacc gaagcgatcg gcatccatga
      481 taatcatgac agtcgcattg ggaacgttga ccccaacctc aataaccgtc gtcgaaacca
      541 gaatatccgt ctttctctcc ttgaaatcct gcatgatctg gtctttttcg tcactcttca
      601 tcctaccatg tagaagagcc acctctgcct tgcctgcaaa atgagtcgtc aactcctctg
      661 ataaggcaat ggcatttttc aaatctagag cttctgattc ttcaatcaaa ggagagatga
      721 cataggcttg ggaacctttt tgaatttccc cctctaacca agtcaagacc tgaggtaatt
      781 gctcatgttt gatccagcgc gtcacaatag gcttccgacc tgctggcatc tggtcgataa
      841 tggaaacatc catatctcca aaggctgtga tggcaagcgt ccgtggaatg ggagtcgccg
      901 tcatcatgag gacatctgga ttgtcgcctt tttcccgtaa aatacgcctt tgccccacac
      961 caaaacggtg ctgctcatcg ataataatca aaccaagacg agcatactcc accccatctt
     1021 gtatcagagc gtgggttccg ataatcaaat cggcctcacc cttggcaatg gtctccaaga
     1081 cctctctctt ttctgcagct ttcaaggaac ctgtcaagag agccagtttc aaattgggaa
     1141 aaaggttctg taaactctca aagtgttgct ctgcgaggat ttctgttggt accattaggg
     1201 cagcctgata acctgctgtc actgccgcaa acatggccaa gccagcgact accgtttttc
     1261 cactccccac atccccttgt aggagacgat tcatgtggtg gtcggacttc atatcagtta
     1321 aaatttcctg caaactcttt tcctgagctt gggtcagggc aaaaggaaga cttactttaa
     1381 ctgctgtcac tttttcctga gaccaattca gaaccagacc acttccctga actctatttt
     1441 cagacttgag catctgcagc tgcatttgga aataaaagag ttcctcaaac tttatacggc
     1501 gaagagcctg cttgtattct gccaaatcct ttggaaaatg catagctcgg actgcctgac
     1561 aacgggacat gagtttgtat ttgtctagta aagactgggg cagattttct tctatcaaga
     1621 ggtctagtcc ctgatcaaaa gccgtcttga tgaccttaac cagactggcc tgactgattc
     1681 cctgagccag acgatagaca ggctggaggt catcttctac ctgagccaga accttcatcc
     1741 cagtcagact agccttagcg cggtcccatt ttccaaagac agcaagagtt gctcccaact
     1801 ctattttatc agccagatag ggctggttaa agaaattcac cgcaaaaacg acctctccct
     1861 gcttgagact aaaacgcagg cgattgcgct tgaaaccata atactggaca ctagcaggag
     1921 tcactacctg accagaaaga actgccttct caccgtcttc tagttccagc acctgcttgg
     1981 ttttgaagtc ttcataacgg aaaggaaagt agagcaagag atcttgcaag ttttcaattc
     2041 ctagtttggc gtatttttct gctgactttg gtcccacacc aggcaagaca tgcaagggtt
     2101 gatgtagatt catgctccac tcctttcttt tctaataata ttctctcgga atacggtcgc
     2161 tgaggaggca aaccacctca tagttaatgg tgacgcggta ggtcgctacc tgagttgcag
     2221 tgatttcctt atccccattg gagccaatca aggttacctt ggttcctagc ggataaagct
     2281 taggcaatcg aatagtgatt tggtccatcg aaaccctgcc gacaattggg caagcttggc
     2341 catctaccaa gacagagaaa ttttgcatgt ctcttgtcca tccatctgca tacccgattg
     2401 gcacggtcgc gatgacttgc tcgctatccg cttgataagt tgctccatag cccatgcaag
     2461 ctccagctgg aactgtcttg acatgaacca gagcagactc caaggtcaag gccggtatca
     2521 aatcataagg caaatccaag accgctccac ttggattgag gccatacatg gcatctccca
     2581 tacgaaccgc attgaaaata gtctctacat gccaaagagt cgttgcagaa ttgctagcat
     2641 gaaccagctc tggaacttcc ttcatactag ctaaaatagt tttaaaccgt tctaactggg
     2701 cattaaaata gtcatctgat tcctcatcag cagtagcaaa gtgggtaaag attccttcaa
     2761 cacaaacacc gtgtttttgg agcaaatctt gagcctgctc aacctcactg acctctctaa
     2821 aaccaatccg tcccatccct gaatcaatct tgaggtggac tgtcaatcca gttaggtcca
     2881 cttccttatc taagagtgct tgaatccact ccagtccagc cactgtcaag gtgaagtcat
     2941 attctttagc tagagcaaca gcttcgattt cagaaactcc taaaatgagg attggcttgc
     3001 tgagtccagc ttgtctgagt tcaatggctt catcgatatt ggaaacgcaa aagccatcaa
     3061 catcatcttg aattgccttg gcaacggcaa cagctccatg accataagca ttggccttga
     3121 ccacagccca cttgagcgtt ccttgaggga tatgagcccc catttgctga atattttgtc
     3181 gaatagctcc cagatgaatc agagccttgg ttggtctatg tggactagct ttcatgattt
     3241 tcctccaaaa tgacactggc tgtcacaaac tgatcggtgt ggctgataga cagccaaatc
     3301 tttcctgaaa atggtgcctg actaaaataa ggcgccccac gttcattgtt caagacttcc
     3361 aaatcctgaa aaccgagctt gctaatgccc gttcccatag ccttggaaaa ggcctcctta
     3421 gccgaccagc gaccagctaa atattctatt tgcctgcgtc ctttgagact ggtgaagcgc
     3481 tccatttcct gagcggtcag tacacgctta gcaaatcctt catgtcgtgt aactgcgctt
     3541 tctatcgaag ccaattcttc gatgtcaatt ccgtgtccaa ctatcattct catcaaaagg
     3601 agttgagatt ccccaactcc atccttttca cttattttgt caaggtagca tagatttctt
     3661 ctaccaaggc ctctgtattt tcccaaccta ggcaagggtc agtaatagag caaccaaaga
     3721 tttctggttg gttttgacga ccatctgcta ggtaagattc aatcataaat cctcgaaccg
     3781 tctttttaat tttctcattc caatcacgat tctgcaaggt ctggcgaaca attcgaatct
     3841 gctccatata ttgcttgcct gagttatcat ggttggtgtc aatgaggata aaaggatttt
     3901 caagtcccat ggtttcatag cgttcaatgg cttggagtag gttttcatag tagtaattcg
     3961 gcatataatt gccatactcg ttgactgctc cacggaggat aacatgggcc aaaggattac
     4021 ctgatgtctc aacttcctgc ccatgataaa ggaaggtttg cttgttttga gcagcataga
     4081 tggcgttaaa cataacaccc aaatttcctg aggttggatt tttcatccct actggtgcat
     4141 caatcccaga agccacaaag cggtgctctt ggtcttccac agaacgagct ccaacggcat
     4201 ggtagctgac caagtcatcc accaagatca gatttgacgg ataaagcatc tcatctgccg
     4261 ttgtcaaacc agtctctgta atcacgcggt agtgcaactg gcgcacagcc tgcaagccgt
     4321 taatcaggct tggagcctta gaagtatctg gctggtgaac caatccttta tagccgtctc
     4381 cattggtacg aggcttagca gtatacacgc gcatgaccat gaaaatcttg tccgctacct
     4441 tcttttgcaa ggcggataaa cggcgagcat attccaagac cgcctcttca ttatcagaag
     4501 agcaaggacc aatcaccaag agaatacggt catcttcccc tgaaataata tctgccaatt
     4561 ccctgtcacg gctttccttg agtctcaagg cttccgcaga caattgggtt tcagccttga
     4621 tgacttccat atcgatttct tgaccttttt caataaatgc catcttattc tcctaacgtt
     4681 ttgtaaattt ctctgacaag agcttctgtg ttatcccaac ccaggcaagg gtctgtgata
     4741 gacttaccaa atacttctgg ctcattttgt cgaccatctt ccagataaga ctcaatcata
     4801 aaaccacgaa cgaactgctt aattttttca ttccaagcac ggttaatcaa ggtctggcgg
     4861 acaattcgga tctgttcaat atactgctta ccagaattgt catgattggt atcaatgatg
     4921 ataaaaggat tttccaagcc cattttctca tactgggcaa tggtatcaat taaattgtca
     4981 taatagtagt tgggaatatt ttttccatac tcattaagag caccacgaag aatagcgtgt
     5041 gaaagcgggt tcccagttgt ttctacttct tttcctaaga aaaggaaact ttgtttgttt
     5101 tgagcagcat aaatcccatt aaacatgaca ttgagatttc cagaggttgg atttttaaaa
     5161 ccagtagaaa atcctgcccc acttgccaca aagcggtgtt gctggtcttc aactgaacgg
     5221 gcaccaactg ccatgtaaga aatcaaatca tctacaagcg gaaggttttc aggataaagc
     5281 atttcatcag cagttgtcat ccctgtttct gtgatgacac gatagtgaag atggcgaacg
     5341 gctttgattc cattgataag actaggcgct tctgtcgcgt taggctggtg aatcaagccc
     5401 ttatagccat ctccgttggt acggggtttg gcagtataaa cacgcataac cataaagata
     5461 cgatctgcca cttcttcttg taggactgcc aaacgcttag cgtattcaag gacagcttct
     5521 tcgttgtcag atgagcatgg cccgattacc aagagaattc gctggtcttc tccacgtata
     5581 atggcttcta gctcttgatc tcgctgtgat ttcctctcca aagcctgacc ttctaatttt
     5641 gacaaggcac gaacttcttc tatatttatt ttagagcttt ttgctgcaaa taccataact
     5701 catctcctta taaagcaaac ggatacccag taaaaattta cttttcacaa gatatccgcc
     5761 tctaaactat ctcattttat tgtcttttac cgtgacagtt tttaaatttc ttaccagaac
     5821 cacatgggca aagttcattg cgtccaatct ggctcaaatc caaatcttct ggcatacttg
     5881 cttggtgagc agcgatattg cgagtcgctg ttgtactgat atgacgttct gcctgtggtc
     5941 tttcttgttc atgaatttgt gctttcatca tcaagcgtgt cacatcaaac tcaatcgaac
     6001 caatcatatc attaaacata cggaaacctt ctgcctgata ctcaacaaca gggttgttct
     6061 gagcatagcc acgaagtcca accgcgttac gcaattgatc aagggcatcg atatgatctg
     6121 tccacttgtt atccaccact cgtagaatca aaactttttg gaattcttta actgcttctt
     6181 catcgcgtag ttttgaaacc tgactatcgt aaaccttcaa ggcacgttgg aaaagctctt
     6241 ccttgatggc cttatcagac aagcctgaca agtcttccat cgtaatagaa tcttcaggaa
     6301 gcaagttgta cttagcaaag ttcaaaattg cctctagttt ttcatcttgt ttggcacgcg
     6361 catgaccatc aacgacacgt tcaatcgtgc gtttgatcat agactgaatt tcaggtgcca
     6421 agtcacgatc tgcagtgatg acatcgtaac gttgagcata gataatctca cgttgttcac
     6481 gcatgacatc atcgtattga aggacttgtt tacgggtatc gtagttattt ccttcgacac
     6541 gtttctgagc tgcttcaacc tgacgcgtca acatgcgaga ctcaatcgcc tcttcagaca
     6601 tgttcaagcg ttcaaagatt cccttcaagc gttcagaacc aaaacgtttc atcaaatcat
     6661 cttcaagaga taggtagaat tgtgactcac ctggatctcc ttgacgacct gaacgtccac
     6721 gaagctggtt atcgatacga cgactttcat gacgttctgt accaataaca caaagtcctc
     6781 caagttcacg aacaccttca ccaagcttga tgtcggtacc acgacccgcc atgttggttg
     6841 cgatggtaac ggcaccacgt tgaccagcat tcatgatgat ttgggcttct ctatagtggt
     6901 ttttggcatt caagacttcg tgaggaacac cagctgcaac caatttctta gaaatgtagt
     6961 cactagtttc aaccgctact gtaccaacca agacaggttg acccttttgg taacgagcct
     7021 taacgtcttc gacaaccgct ttaaacttag attctatact tgcataaaga aggtctgagt
     7081 ggtcaatacg ttgaacagga cggtttgttg ggattggaat aacacgaatg ttgtagattt
     7141 cacgaaattc ttcttcctca gtcttacctg tacccgtcat accagacaat tttttgtaca
     7201 tacggaaaag gttttggtac gtgattgagg cagatgtctt ggtttcatcc tggattggca
     7261 caccttcttt ggcttcaata gcttggtgca atccatcaga ataacgacga ccttccatgg
     7321 tacgacctgt aaattggtcg acaatcaaga tttcttgctc ttcgctcacc acatagtcaa
     7381 tatcgagaag catgatgtag ttggcacgaa gggcgttatc gataaagtgg gtcaaagcca
     7441 cgttttcgat gtcatagagg ttttcaagtt tgaagtagct ttcagccctg tcaatccctg
     7501 aatcagacaa accaatagtc ttagactgca catcgatgat gtagtcatct ttgttcaaag
     7561 attttacata gtggtctgcc atgtgataca actgactggt ttcaaccgca ttggcacctg
     7621 atacaatcaa aggtgtacga gcctcgtcaa tcaagataga gtcaacctca tcgaccaagg
     7681 catagttaag cggacgttgt accatgtttt cagcgcgaac gaccatattg tcacgaaggt
     7741 agtcaaatcc gatttctgag ttagttgagt aagtaatatc acactcatag gcttcttttt
     7801 tctccattgg agatttggta gccaagttaa tccctactga caaaccaagc caagagtaca
     7861 attcacccat ctcagtcgcg tcacgttctg acaggtattc attaaccgta actacgtgaa
     7921 cccctttacc tgaaagggca ttgaggtata ccggcatggt cgcagtcaag gttttccctt
     7981 cccctgtacg catctctggc acgtcaccat ggtgaagaac aatccccccc atgacctgaa
     8041 ccttataagg gaagagacct aggacacgtt tggcaccttc acggacaacc gcaaatgctt
     8101 cgtaaagcaa tgaatccagt gattctccat tttgataacg ttccttaaat tcaactgttt
     8161 ttgcttttag ttggtcgtca gtcaaagcag ccatttggtc ttcgtatttg aaaaccttgt
     8221 cagccatctt ttccagacga cggatttctc ctttatcatt ttcgataatt gtttttaaaa
     8281 tattagccat gtttttcctt actttaaatt ccgaatattt tagaatgttc ctttaatttt
     8341 agcacaatta ctgattattt tcaaggaaga atccccattt tgaaaaggaa aaagaggtta
     8401 gttttcaagc taacctctcc gacttttatg actgtttaat tgccaaaaat cggcaaaatg
     8461 gcaaatgtga taaataagtt aatccaaaga gaaagacagc aaatcagtcc aaaaacaaag
     8521 agactgactt tcttggacag caaggccatc aaaatggaca gaattccaaa aactagcaag
     8581 actttctgta tgatgaagat gtcaatcctt acccccagaa tcgctctatc ccaagggata
     8641 aagaagaaag acagccagat cagcagaact aaaccaatat aaaccttgga atagcgtgta
     8701 ataaatgatt ttagatgtgc cataagccat ctcctaggat tgtatatcct ttacatattg
     8761 tatatgtcca ttttcgacaa ctaatacatc tgtacaaagg tctgaaatat cttccgatat
     8821 gtgcgacgtc aggataacca gaccattttc ttttttcagg taagataaaa tgacctgttt
     8881 ggttaacctc acacttttct catccaaagc attcataggt tcatcgagaa agagtataga
     8941 aggagaggaa ataaaggctt gaatcaaggc cattttttgc tttgttccca aggataaatg
     9001 ccggtattca acgtcttcaa attcctgtaa atcatagtat tgaatccaat aggcaattct
     9061 tttttccgta actttagatg aaaaatacct taacagttcc aaattttctc tcagggataa
     9121 atgagataaa aactcgactt tttcaattaa aattcctgca tcctgaatat aatgattttt
     9181 tatcccgtag actttcccgt cttgaagaac ttttcctttg tcaagcttaa tataaccagc
     9241 aagtaccttt aaaagaaccg tcttgccaga tccattatca cctttaagtc cataaatttt
     9301 tcctgattga aacaccaaat tcaactgatt taaaataggt cttttcccat aaattttctg
     9361 tccatttata atttcaagtc tcatcgttta tcctttctta aatttctctt ccaagcgact
     9421 aagaatcaac ataactaata aacttaagag tagtaaatac actgttacaa tatgatccat
     9481 catccattct tgtcccacaa atgctaaaaa atagagtaaa aaggaaaaac cagcgcccgc
     9541 actaatcagt gctatcagat aagacagtag taagtacaac atcattaact gagcaagtaa
     9601 aaagaaaagt gcgactgttt tgaaatgaat tcctagagga agtaaaccaa gaaaccagac
     9661 aaacaaatct tgagtaaaaa atttagaaat gacaaccaat cgtttccttg tataatcgaa
     9721 aaaggagctt gattgatagc gaatcaagtg atggtaatgg tctgccaaat cactttcaag
     9781 taaaatgata gagagccatc ctaccatgaa aagcgaaagg tttaacaaga gtttggggat
     9841 ttctaaattc gcttcagaat agtcattata ggaaaaaatt gaaatcaatt ctctcttgaa
     9901 aaagataaca gcatcctgct tttgaaccgc tgttgacaat agatggttta tcaaaatcat
     9961 catcagaact atacgaagta gtaaaacaat ctttctcttt agcaaaatat tgttaaaatt
    10021 cttaaacatt atcttcatat tttttaagta ccatataagg gacaacataa aggatataag
    10081 gagtaaagag tttcatcaaa cttacatccc catagctagc ttgcatcaaa ctcgtcatag
    10141 gaaccatata gtaaggcaag gtactataga gtaatataga accaagccaa agaaacatca
    10201 aataggcgat tgccgaacga gtcacattcc ccacataatc aactatttgt aaaaaataga
    10261 ttgcattgat gaaaataccg attagtagga cacaagtaaa gaacaaatag ctttttatct
    10321 ctccatctag gagtctttgt aaaccacttc catcagaaaa tagagaattc catccaagag
    10381 gagaaaaagt cccaaggaaa taggttataa ttgcaattgt cagcacagtc actaaatata
    10441 tcaaacaagg gatacttgca acttgcaaag tcaactttct ttttaaccct tgatagctca
    10501 cttctcttcc aatactcaat cgtaaaactt tatttttcag ctcaatatat tgaaaaccta
    10561 gcaaaacaat gatgattgga ataataaatg attgataaaa acctagattg aactggagaa
    10621 tacgaaaatc taatgctgat tttaataagt catttttaat tgcttgtcca tcgaatttag
    10681 ctagatcggg cgcatgaata tcaaaaaaga aataagtcat caaaacgtca cgataaacca
    10741 aagcaagaaa aagcaaagta agtaagacat aaacaaattt atgccttgcc aaagttaaga
    10801 gaggaatctt cataagccac ctcctacaaa tagaaagtaa tataatactc aatgaaaatc
    10861 aaagagcaaa ctaggaaact agccgcaagt tgctcaaagc actgctttga ggttgtagat
    10921 aagactgacg aagtcagtta catatataat ccaaggcgac gttgacgtgg tttgaagaga
    10981 ttttcgaaga gtataaatca atgccttccg cctttagatt tccctagttc ccgcctcaag
    11041 cgaatcattt ttttgaaaaa gacataggct aaccaatcac tagcaaaagc gataagtagt
    11101 aataatagcc aaatacccca taacttgata tctgtcaaat ttctcaagac gatattgaaa
    11161 aacagaactg aaaaaactgt ccaagcaagg ctaaaaagag catagagggt gatgtaaatc
    11221 cagtaaaaat agtaaaaaat tgggaaaaat ttactttctc tattatcctt gtcaatccag
    11281 ctgaaaaagt aaaaacaggg taataagatt atcattttaa acaagtgttc catcacaaac
    11341 ctcctctttt aaattgtttt ctttagtttc gttctattaa atgttattac caatataaac
    11401 cttggaatag cgtgtaataa atgattttag aggtaccata aggccacctc ctacaaatag
    11461 aaaccgatat aaatcaatgc cttccaccct tagacttccc tagttcctgt ctcaagcgaa
    11521 acatttcttt gaaacaggaa taagttaacc aattcatacc aatagctagc agaataaaaa
    11581 gaaaccaaat gccccataac ttgatatctg tcacatttct caaggcggta ttgaaaaaca
    11641 gaactgaaac aactgtccaa gcaaggctaa aaagagaata gaaggggatg taaaaccagt
    11701 aaaaataata aaaaattgga aaaaacttac tatttctgtt ggccttctca atccagttat
    11761 caaaataaaa gtacggtgct aaaagtaaga atttaaacaa atgttccatc accgacatca
    11821 ccctctttct tttgatagcg ttttctatta ttttattata tcaaaaaaat ccggaactgt
    11881 cattccagat tctacttttt tatttgcgtt ttcttgcgat gagatgaatc ggtgttccct
    11941 caaaaacaaa ggccttgcgg atttgatttt ccaagaaacg caggtaagaa aagtgcatga
    12001 gttcttcttc attgacaaag atgacaaagg ttggtggttt ggttgccact tgggtcgcat
    12061 agaaaatctt gagacgtttt cctttgtctg tcggtgttgg gttgatggca atggcatcca
    12121 tgatgacatc gttcaagaca gctgatggaa tacgtgtatt ttgactttcg ctgatttgct
    12181 taatcatctc aggaagtttg tggagacgtt gcttggttaa agctgataca aagataatcg
    12241 gtgcgtaagg caggtattgg aactgctcac ggatatcttc ttcccagttt ttcatagtgt
    12301 ggttatcttt ttcaagcgta tcccacttgt tgaccacgat aatcatccct ttaccagctt
    12361 catgggcaaa tcctgcgata cgcttgtcgt actcacgaat gccttcttcc gcattgatga
    12421 ccatcaagac cacatctgaa cggtcaatag cacgcatggc acgcataaca gagtatttct
    12481 cagtattttc ataaacctta ccagacttac gcataccagc cgtatcaatc atggtaaact
    12541 cttgaccatc tgtatctgta aagtgggtat caatggcatc acgagttgtt ccagcaacag
    12601 gactagcaat aacacggtct tctcccaaga tagcattgat caagcttgat tttccaacgt
    12661 taggacgacc aatcaagcta aacttaatga catctggatt ttcttcctca tattcatttg
    12721 gaagattttc tacgatcgca tctagcacat cccctgtacc gattccatgg acagatgaga
    12781 taggcaatgg ttcacccaaa ccgagagcat agaaatcata tatatcattt ctcatctcag
    12841 ggttgtccac cttgttgact gcgaggataa ctggtttgtg ggtcttataa agcttacgag
    12901 ctacgtattc gtctgcatca gtaattcctt ccttaccaga cacgacaaaa acgataacat
    12961 ctgcttcttc catggcaatt tctgcctggt gcttgatttg ttccatgaaa ggagcatcga
    13021 catcatcaat tcctcctgta tcaatcatgc taaaagaacg attgagccac tcacccgttg
    13081 cataaatacg gtcacgtgtc actccttcga catcttctac aatggagatt cgctcaccag
    13141 cgatccgatt aaatagggtt gatttcccaa cattgggacg tcctacaatg gcaatagttg
    13201 gtagggccat aatttctcac tttctacaat aatttcttct gttcaagatt ttttctagtt
    13261 gagcttggtt cagcttgacc aaactgttct gctaggcgct gactccagct tgtggtcgca
    13321 cgcgccccag catagtcagc ctgaacacgg tcataagctt ggattgcctc agttgactgt
    13381 tcttggtatt cttcctcaaa gacaacattc tctagtggca gtctcggttt catatcatga
    13441 tgttgatttg gcacacccag tgccatccca aagacagaat aggtgtagtc aggtaggtta
    13501 aagagctctg ccacttcttc agacttgtat cgaaccaaac cgataatcac accaccatag
    13561 cccaagcttt cagctgccaa caaggcgttt tgtccagcaa gagctgcatc gaccgaacta
    13621 atcaagagac cttccacacc ttggggttgg aaggtgtcgg tatgaagtcg ggctcccttt
    13681 tctgctcggt tcaaatctcc gacaaagaga aggaaaacag cagactggcg aatggcttct
    13741 tgaggtacca attcatacaa ggcatctttc ttctcttgac ttcgtaccac aatcacagag
    13801 taggattgga aattcttcca agatgatgcc atctgggctg ctgtcaaaat ctcatttaag
    13861 tctacttggg gaatttcttg ctctttaaac ctgcgcactg aagtatgagc cttcatcaat
    13921 ttaatggttt ctgtcatcga cggtttactc cttctaaacg agtctcctca gccaaataac
    13981 ggatgcgttc catgacccgt ctggcttccc aggtttcgtc atttccatgt ttcactttcg
    14041 caaaatgctt ctccaaatct tcaaagttga agttggatgt gaaaaaggtc ggtaaatttt
    14101 cctgcatccg atattggaga atgacctgca ggatttcgtc acgcacccaa acggttgatt
    14161 gctcggcgcc aatatcatct aaaatcagga cctcagaaag cttaatctca tccaccaagg
    14221 tcttaacatt gccatcactg atagcatttt tgacatcaat gacaaagcta ggatagtgga
    14281 ggagagttga tgaaacacca cgtttttctg ataaatcatg agctaaggcc gccaccatga
    14341 aacttttacc cacaccaaag tctccatata agtaaagacc ttttcgaata gctggatatt
    14401 gctccacgaa ggctagtagc ttttcaaaaa ctggtaagcg ccccaaatca tccaagtcaa
    14461 cttgagccaa actagctttc ttgagactgg ctggtagatt gattaacttg agacggttct
    14521 taatagccgc ttctttttca gccgcaatta gctcaggagt ttcttcatat gaaacatctg
    14581 cataaccatg attcttaacc aaaatcggct tgtagccttt ggcaatataa tccgtatccc
    14641 cacggagaaa cttgtcacgc tcggtgatgt actgattaaa cttggagata ctgcgattta
    14701 attcctttgg agttaaggat tcttgctgga taaaggccgc aacatcaggg tccttcatga
    14761 ttttctggac caaatcttga taataaaaac ggctgggttg acgtttgagt acgtctccga
    14821 cactttccat ctaatctcct cctttttcta atcgagctaa tagttcttgc ttcttacgtt
    14881 ctagttccag acgagtttcc tcgctggttt cattcttata ttcaggatta ctccatttag
    14941 gaacattggt tttttctggg gcagtctgat tctgtttttg tgtttttgct ttctgccctc
    15001 gatcacgaat tcgtaaaacg gcctcttctg ccgaatgaat cttttgatag gcatagtcat
    15061 tggctacctt catggcatat ttctcattga tatttgccga atccacctta ttaaaggtca
    15121 ataagagaat aatattgatg acttcgtcca gtaagcccaa gccagccatc tgttgcaaga
    15181 gttctctttc tgtttgggta atggttccct tgcgtgtttg cttgatttct gctaagaact
    15241 gcagggcagt tttactttta gcttctttga taatggtcgc ttccttaaga ctaaagtcag
    15301 aggaaactgg tttttgagca attttttcac gcatgcgttt ggttgaaata acctgggaaa
    15361 cagctgttga cttggccaat tgataggttt caaaccaagt ccatttcttc tcctcggcaa
    15421 tagcaaagag gtttaagaca tcggactgct catccgcaaa acgaagtcca tctcgagcca
    15481 tcagctggcg aaaatgttcc aagtcaaaat cattggccac tttcttcttg agaccaaggt
    15541 cttcttgact gcctagttct gccaattctg gaaagacttg attgagtgag acaggtattt
    15601 cttcaccatc agcactttca actttcaaat cctccacagc tacatcgcca atctttttct
    15661 ctaagagtct gcgataaaca ggatgcccca agaagtcttg actagataga ggagcatgga
    15721 aggctagctg ataaacatca cccttttgat agagggtcaa gagattaaaa gcagataaga
    15781 ttttcaatga ttttatcagt ctatccatcc caaagttgag atgattgagg atacttgaaa
    15841 aaagatattc ctttctacca ttatcccaaa aactgattgt ataaagataa aggctcagtg
    15901 cctcctgacc gataatcggg aggtagcact gtaccagaga tgaggtatct tgcgacaccc
    15961 gattattctt tagataagaa aaacggtcaa ttggcttcat ttatctttcc tttttctttt
    16021 tagaggactg ggtgatttgt tggagcaagc tctctaactc actgacatcc ttaaaactac
    16081 gatagacact agcaaaacgt acataggtaa tctcgtccaa ttcagccaac tcctccatga
    16141 cgagtgaacc aatgtcctca ctttgaattt cattttcatt tcgaccacgg agtttctgtt
    16201 cgatacgatt gactaccatg ttgatttcat cacttgacac aggacgtttc tgggctgagc
    16261 ggataatccc attaaagatt ttatctctgg agaattgttc ccgtgtgcca tcttttttaa
    16321 caaccactaa ggttctttct tctactcgtt cgtaggttgt aaaacggtgt tggcattcgt
    16381 cgcactcacg tcttctacga atggtgttcc cttcttctgc ttggcgacta tcgataacac
    16441 ttgacttggt agccccacat tttggacaac gcatcctttc cctccttatc gttttctttt
    16501 cattatacca ttttttaagc gattcccaaa acaattcttc tttttgcttg acaagttttt
    16561 tgttttgttg tattatttaa ttaagacaac aaggtaaaag aaaggagact aagatgtcct
    16621 ggacatttga caacaaaaaa cccatctatt tacagattat ggagaaaatc aagcttcaga
    16681 ttgtttccca tacactggaa cccaatcaac aacttccaac cgtgagggag ctagctagcg
    16741 aggctggtgt caatcccaat accatccaaa gagccttatc agaccttgaa cgagaaggat
    16801 ttgtctacag caagcgaaca actggacgat ttgtgactaa ggataaggag ctaatcgccc
    16861 agtcacgcaa acaattatca gaagaagaat tggaacactt cgtttcctcc atgacccatt
    16921 ttggctatga aaaagaagaa ctaccaggcg tagtcagtga ttatattaaa ggagtttaag
    16981 cctatgtcat tactagcatt tgaaaatgta tccaaatcat atggagcaac accagccctt
    17041 gaaaatgttt ctcttgacat tccagctgga aaaattgtcg gccttcttgg gccaaacggc
    17101 tcaggaaaaa caaccctgat taaactaatt aatggcctct tacaaccaga tcaaggacgt
    17161 gtcctcatca acgacatgga cccaagccca gcaaccaagg ccgttgtagc ttatttgcct
    17221 gatacgacct atctcaatga gcaaatgaag gtcaaagaag ccctaaccta cttcaagacc
    17281 ttctataaag atttcaatct tgaacgcgcc catcatctac ttgcagacct gggcattgat
    17341 gaaaatagtc gtctcaagaa actatcaaaa ggaaacaaag aaaaggttca actgattttg
    17401 gttatgagcc gtgatgctcg tctctatgtt ttggatgaac ccattggtgg ggtggatcca
    17461 gcagcccgtg cttatatcct caataccatt atcaacaact actcaccaac ttctaccgtt
    17521 ttgatttcta cccacttgat ttctgatatc gagccaatct tggatgaaat tgtcttccta
    17581 aaagacggaa aagtcgtccg tcaaggaaat gtagatgata ttcgctacga gtcaggtgaa
    17641 tccattgacc aactcttccg tcaggaattt aaggcctaag caaaggagat tatttatgtt
    17701 ttggaattta gttcactacg aatttaaaaa tgttaacaag tggtatttag ccctctacgc
    17761 agccgtgcta gtcctttctg ccctcatcgg aatacagaca caaggcttta aaaatctacc
    17821 ttaccaagaa agtcaggcta ctatgctact ttttctagct acagtctttg gtggcttgat
    17881 gcttacactt gcgatttcaa ccattttctt gattattaaa cgcttcaaag gtagtgtcta
    17941 cgaccgacaa ggctatctga ctttgacctt gccagtttct gaacaccata tcatcacagc
    18001 caaactaatc ggtgccttta tctggtcatt gattagcacc gctgtattgg ctctaagtgc
    18061 tgttattatt ctggctttaa cagctccaga atggattcct ctttcttatg tgattacatt
    18121 tgtagaaaca catctccctc agatctttct tacaggtata tccttcctac taaataccat
    18181 ttcaggaatc ctctgcatct acctggctat ttccattgga cagcttttca atgaataccg
    18241 tacagcactc gctgttgcag cctacattgg tatccaaatc gtcattggat ttattgaact
    18301 tttcttcaat cttagttcta atttctatgt caattcactg gtaggactca atgaccattt
    18361 ctatatggga gcaggtatag ccattgttaa agaactcata ttcatagcta tcttttatct
    18421 cggaacctac tacatcttga gaaataaggt taatttgctt taaataattt ttacctagat
    18481 atgtaacata ctcatagaac aaaagagacc aggcaaaaag tttttaaaat tagaaaacgc
    18541 atagtatcaa gtgttaaaaa tccttgatat tatgcgtttt ttgtgagctg acttatttcc
    18601 tttcactata tcgcaaaatg aaataagaac ggaacgatgg gattttggaa ttcaaatcaa
    18661 tttataagaa tgttttagaa gtaatattat cctattccag attcagttca ctatacaatt
    18721 gagttttcaa gcaacctgtt tacataatgt gtacataatt aggttcgtga ttccaccctt
    18781 ttcaccttta aaaacctcgc tttcgcaagg ctcttctatt tataagataa ggcacgttta
    18841 aaggttttcc aaatccctaa atcatccgtt tgaagaacga gactagcata catgcgtccg
    18901 ataaatcctg ttgctaccac cgcaaaaatc actgtaatag caagtgaaat ccatgcttct
    18961 gctccccccg catagccatt aatcgttcga aacggcataa agaaggtcga aataaaggga
    19021 atataagaac caatcttcaa gaggagattg tcaccagctg cacctagagc tgtcactcca
    19081 aaaaaaccac ccataatcaa aatcatcaaa ggcgacaagg ctttccctga gtcctcagga
    19141 cgagaaacca tagatcctag gaaggctgcc aagactacat acatgaaaag actgatcaaa
    19201 ataaagagca aggtattcag tgagatagca tctcccaagt gatccaaaat accagactga
    19261 gccaagaatg gcaaatcttt aaagagcaaa acggcagcca gaccacctac aacatagatc
    19321 ccaatatgcg ttaaaatcac tagaaacaga gccatcatcc gcgcatagaa atagtgactt
    19381 gcccttatgc tagaaaaaac gacttccata attttggtgc ctttttcact ggcaacttcc
    19441 tgagctgtta cacccgcata ggtaatcaga atcatataaa gaaagaatcc taaggcacct
    19501 gctgcaattg tttgaataaa ctttttattt tccttggctt catcaatctt ttctgtgaat
    19561 tgaattgtct gcgctaagcg tttttcctgc tcttgagaca aggaagcagt tgaacgatta
    19621 agctgatttt gcagttcatt gagtgtacct gtaacctcaa atttaattcc attttcaagc
    19681 gatgtttcgc catgataaac tgcctttaga acactatctt cttgatcaat ggtcaaataa
    19741 ccttttaatt tttcttcttt aattgcttct ttggcacttg cttcgtcttt atagtcgaag
    19801 ttaacaccat ttacattctt cagtccttct gctacagatg gcactgttgt cactactgcc
    19861 actttattat ttttagccat agaagaacct tggatatgcc caattcctac agagattcct
    19921 aaaaagagga acggcgaaat caccataaag aagaaactcc atgacttgac atgtcgaaga
    19981 taggtttcct tgattacaac ccacatattt ctcatacttc cactcctgat tctagtttaa
    20041 agatttcatc gatagttggc gcttgttggt caaatgttgc gatatattga ccttgagtca
    20101 agattgggaa gagttccctt ccagcgctct catcctccaa aatcaatttc caactgcctt
    20161 gtttggtcaa gctcacctgt ttgacatgag gaagattttc caattcttcc ttgcttcgtt
    20221 cacttgaaac aaagagacgc gttttcccgt attgattgcg gacatcctga actggtccgt
    20281 gcaagaccac acggccatct cggatcatca gaatatcgtc acaaagttcc tcaacattgg
    20341 tcatgacatg gtcagaaaag ataatggttg ctccgcgctc tttttcctga aaaatgactt
    20401 gtttgagcaa ttctgtatta actgggtcca atccactaaa aggctcatcc aagataatca
    20461 ggtctggttc atgaatcaga gtaataatga gctgaatctt ctgctgattt ccttttgaca
    20521 gactcttgat tttatctgtc agctttcctt tcacttccaa cctcttcatc cattgaggga
    20581 gtttttcttt gacttctttg gcatccatgc cttttagagt cgccaagtag cgaacttgtt
    20641 caagaactgt caatttaggc atgagactgc gttcttcagg cagataacca atccgagcat
    20701 aggtctcctg acgaatatcc tgaccatcca gaccgatttc tccctgatat tctaggaatt
    20761 tcaaaatact atggaaaatc gttgtttttc cagcaccatt ttttccgact agtcccaaaa
    20821 tacgacctgg tcgcgcttga aagtcaatac caaacaaaac ttgcttggat ccaaaacttt
    20881 tctctagact tcttacttct agcatctttc acctccgaaa tttcttgcac tcattatact
    20941 cctttttgat agcctttaca atgttttttg tccattttta gaagactatt gctgtgtaaa
    21001 atatggcctg gagcactttt atactcaatg aaaagcaaag agcaaactag gaagctagcc
    21061 gtaggctgct caaagtacag ctttgaggtt gcagataaaa ctgacgaagt cagctcaaaa
    21121 cactgttttg aggttgtgga tagaactgac gaagtcagta actatatcta cggcaaggcg
    21181 aagctgacgt ggtttgaaga gattttcgaa gagtattagt gataaatcca ttatacagta
    21241 gcaaacttaa tttatacctt ccgctcctca actgtctatt tttaatcctg aattgttatt
    21301 tgagtaactc ctttttcctc ataaagtttt cttcctctaa aacttctgga aaaaagcgaa
    21361 tagtttcaga caacattttt ataagaaaca agttcatctg tcatttcaag aaggagtaat
    21421 cctttatcta ctaatggacg gaacggaatt caaccgcttg tccgatatgt tttctaagga
    21481 ttatatagta aaatgaaata aaaacaggac aaatagatcg ggacagtcaa atcgatttct
    21541 aacaatgttt tagaagtaga tgcgtactat tctagtttca atctattata tctattatgc
    21601 acaccactat aggatctaat aaaaatcaca acaagttcat tcatagatgg ttacctcagg
    21661 gaactaagaa aacgactaca aaggaagtcg cattcatcga aaagtggatt aacaactatc
    21721 ctaaaaaatg cttgaactac aagtccccca gagaagactt ctggatgact aacttgaact
    21781 tgaaatttag caataattaa ttcaccatct aactatattt agtaattatt tcagaactga
    21841 ttaatattaa aattaactaa caattcaaag gattcatact agccataaat tacgtccatc
    21901 agagagagac tcttactact tttagatttt agtctttcta gcttcagaat acatctaaac
    21961 tttagggaaa atgactattt gaaagcgcga atgcctcaaa attatctcag ataagctatt
    22021 cgaaacttag aatgctttta aatttatgga attgcgatta ttcgaaacct agaatgcata
    22081 taacctttag ttgacagacc tattctaagt ctcgaagggc tatttacttt ctattcctta
    22141 tcaaaaaaga ctcattcccc ctttctcctc caaaatatgg tatagtagaa atatactatc
    22201 tatgaggagt ttacatgtca caggataaac aaatgaaagc tgtttctccc cttctgcagc
    22261 gagttatcaa tatctcatcg attgtcggtg gggttgggag tttgattttc tgtatttggg
    22321 cttatcaggc tgggatttta caatccaagg aaaccctctc tgcctttatc cagcaggcag
    22381 gcatctgggg tccacctctc tttatctttt tacagatttt acagactgtc gtccctatca
    22441 ttccaggggc cttgacctcg gtggctgggg tctttatcta cgggcacatc atcgggacta
    22501 tctacaacta tatcggcatc gtgattggct gtgccattat cttttatcta gtgcgcctat
    22561 acggagctgc ctttgtccag tctgtcgtca gcaagcgcac ctacgacaag tacatcggct
    22621 ggctagataa gggcaatcgt tttgaccgct tctttatttt tatgatgatt tggcccatta
    22681 gcccagctga ctttctctgt atgctggctg ccctgaccaa gatgagtttc aagcgctaca
    22741 tgaccatcat cattctgacc aaacccttta ccctcgtggt ttatacctac ggtctgacct
    22801 atattattga ctttttctgg caaatgcttt gacacgtaaa aaatccgttt ggtttcccaa
    22861 gcggattttg tgctttattt tgaaacttct tttgcaagaa caaagttccc aagtgtggca
    22921 gaaccatttc ctgcgactgc tggcgtcacg atatagtcac gcacatctgg tactggtagg
    22981 taaccattaa gaagagatgt aaatttctca cggacacggt ccagcatatg ttgttgagcc
    23041 atgacccctc caccaaagac aatcacgtct gggcggaaag tcactgtcgc attaaccgca
    23101 gcttgagcga tatagtaggc ttgaacatcc caaacagggt tgttgaattc aatagtttcc
    23161 ccacgtacac ctgtacgagc ttccaaactt ggaccagctg cataaccttc tagacatccc
    23221 ttatggaaag gacaaacacc cttaaactct ttttcaatat ccattgggtg tctagcaaca
    23281 taataatgac ccatttcagg gtgacccaca ccaccgataa actcaccacg ttggatgacg
    23341 cctgcaccga tacctgtacc gattgtgtag taaaccaagt tttcgatacg accaccagca
    23401 ttgttacggg caaccatttc accgtaagca gagctgttta cgtctgttgt gaagtacatt
    23461 ggcacgttta gggcgcgacg aagggcacca agcaagtcca catttgccca gtttggtttt
    23521 ggagtcgtcg tgataaagcc ataagttttt gagtttttgt caatatcaat cggcccaaat
    23581 gaaccaactg caagaccagc aaggttatcg aattttgaga agaactcaat ggttttatcg
    23641 attgtttcga ttggagttgt tgttggaaat tgtgtttttt ctacaacgtt aaagttttca
    23701 tcaccgacag cacagacaaa ctttgtaccg cccgcttcca agcttccata taattttgtc
    23761 atgataaacc tcttgttttt attttcttta ttatagcata cttcgaaagt ctaaatgtct
    23821 ctatttttta gattttcctc tgtaaatctt actatctaat aaaaacgaac aaacatgtca
    23881 tttgttcgtt ttcacattag agaggattga ttagattttc acttcgatca cagcatcccc
    23941 cttcgcaact gaacctgttg cgactggagc tactgaagcg tagtcagctg tatttgtaac
    24001 gataaccatt gttgtatcat caagtccagc tgcagcgatt ttgtttgagt caaatgttcc
    24061 aagaacatcg ccagctttca ccttattacc ttgagcaact tttgcttcaa aaccgtcacc
    24121 gttcatagat acagtgtcaa taccaacgtg aatcaaaact tcagcaccat ttcttgtttt
    24181 caaaccaaaa gcgtgccctg ttggaaaggc aattgaaact tcagcatcag ctggtgcata
    24241 gaccacacct tggcttggtt tcacagcgat accttgtccc atagctccac ttgagaagac
    24301 tgggtcattg acatcagcaa gagcgacaac atcaccaacg ataggagtta caagtgtttc
    24361 attttgaaga gtatcagtct cttccactac tggtgcttct actgtaggtt ctgtaactgt
    24421 ttctgcggtt gctgtggcag cttcatcagc aaagacacta gctgcttttg ttttcccgta
    24481 gaaataagtg aaagcaaagg cggcaacaaa ggacataatt tccgcaatta tatacatagg
    24541 gattgaacca gccttaatag aaaggaatcc aataaatcca gccgctccca atgaaactgc
    24601 aataacgtga gttaaacctg ctacaaaggc accaattgct gaaccagcaa gggcgcagaa
    24661 gaatgggaat ttgtatttca agtttacccc aaagagagca ggttctgtaa taccaaggaa
    24721 ggcagaaaca gcagccgaac tggacaatcc ttttgtttta gcattttttg tcaagaagta
    24781 gactgcaaga gtagccgcac cttgtgctac gtttgccatt gaagcaacaa caaatatgta
    24841 gtctccaaat ccagtaccgt tattattgta tgcagacaac aattgtgttt cgattgctgg
    24901 gaatgattgg tgaagacccg tcataacgat taatgagtaa gttccaccaa atagccccat
    24961 accaaaggca cctgttgtgt tgtacaacca tacaatcgca tttgtaatcg catcagacac
    25021 aaccaacata acaggaccga taactgtaaa ggtaaggaat cctgtaatca tcactgataa
    25081 aagcggtgta aaagtgaagt caactgcaga tggcaatttc ttgtggaaga atttttctaa
    25141 attagctaac aaccagactg caacaaggac tggaataacc tgataagcat agtttgtttg
    25201 tgtaacatgg aaacctaaga tgtcccagaa ggcttcttta ccacccaaat ctggagtggt
    25261 cataatcata ccgattgccg caccaaggaa ttggtttgct ccaaagcgct tggctgctga
    25321 aataccaacc aaaacaggaa ggaacataaa tggagcagca gacatcaatt gaatcatagc
    25381 tgagaagcct ttgatagcag gatacatatc ttcaattgat tgaggtccaa acaaatctgg
    25441 tgatgtcaag aagttccgta aagccatcaa gagaccacct gcaactaagg cagggataat
    25501 aggaacaaaa atgtctgaca aaagtttaat caagtccatc aatggattga actttttgtc
    25561 tttattagca atctctttca aatcatctgt cgaaacttct ttcaaaccag ttttcttaat
    25621 aatttccgca tatacgaagt taacatcacc aggaccaatg ataatttgat attgtccgtt
    25681 agtgctaaaa gtccccttga catccgcatt attatccaga gctgcttgat tcacttttgc
    25741 ttcgtctttc aataccaagc gaagacgtgt cgcacagtga gctgcagcta caagattatc
    25801 cttaccgaca gcctcaatga cctcggcagc aactttacta taatccatag caaaaatctc
    25861 cttatataac attttgttct tgtttgaaag cgattttatt cgcccttacg actattattt
    25921 tatcatgttt taaaaatatg tcaagcgttt tgcagaaaaa atattctatc cacttagtgg
    25981 gcaagtttta gattgattgt ttttgactgt ttcatcagga attccttgaa aaataagttt
    26041 gaaaccttgg ttaaaaagtc ttttatttca ttatctttca cattttcgta aaaatttgat
    26101 tgatttcctg tatttatttt atgataatcg tttgacagtt tttcctcttt tttaacataa
    26161 aaggcttgca ttttttatcg aaaacagtta ctattaaaag tgataagatt tttggaggaa
    26221 tgaatggaat ggacaactga gcgtcgttac agactttatc aagattggac gcaagaagaa
    26281 attcaacata taaaggaaaa tatggcacaa tctccatggc atactcatta ccatgttgag
    26341 ccaaaaacag gacttctcaa cgacccaaat ggcttttctt actttgatgg caagtggatc
    26401 ctcttttacc agaattttcc ttttggtgca gcccacggtt tgaaatcttg ggcacagcta
    26461 gaaagtgatg atttggttca ctttaaagaa actggaatca aagttttacc agatactcca
    26521 ttagatagcc acggtgccta ctctggttct gccatgcaat ttggcgataa cttattccta
    26581 ttttatacag gaaatgttcg cgatgaaaac tggatccgtc acccatacca gatcggtgct
    26641 ttgatggaca aggagggtaa gattacaaag attgacaaga tcttgattga ccagccagca
    26701 gactctactg accacttccg cgatccacaa atttttaact ttaaaggtca atactatgct
    26761 gtcgtcggtg gacaggactt ggagaaaaaa ggcttcgtcc gtctctacaa ggctgtcaat
    26821 aacgactaca caaactggca agcagttggc gaccttgact ttgctaacga ccgtactgcc
    26881 tacatgatgg aatgtcctaa tctggtcttt gtagaggaac atcctgtcct tctctactgt
    26941 ccacaaggat tggataagaa agttctagac tacgataata tctttccaaa tatgtataag
    27001 attggggctt cctttgaccc taaaaatgcc aaaatggtag atgtgtctca acttcaaaac
    27061 atggattacg gtttcgaagc ctatgcaact caagccttca acgctcctga tgggcgtgct
    27121 ctagcagtta gctggcttgg tttgccagat gtttcttacc catctgaccg ttttgaccac
    27181 caaggaacct tctctttggt caaggaactc actatcaaag acgacaagct ctaccagtat
    27241 ccagtcgctg ctattaagga ccttcgtgct tctgaagaag ccttctcaaa ccgttcccaa
    27301 accaagaaca cttacgaact tgaactcaac ttggaagcta atagccagag cgagattgtc
    27361 ttacttgctg ataaagaagg taagggactt tcaatcaact ttgaccttgt aaacggtcaa
    27421 gtgacagtgg atcgtagcca ggctggagaa cagtatgccc aagaatttgg gacaactcgt
    27481 tcttgcccta tcgagaatca ggctactact gctacaatct tcatcgataa ctctgtcttt
    27541 gaaattttca tcaataaagg agaaaaagta ttttctggtc gtgtcttccc acatgcggac
    27601 caaaatggta tcctgatcaa atctggaaac ccaactggaa cttactatga attagattat
    27661 ggtcgcaaaa ctaactgatg tcgccaaact tgcaggcgtc agtcctacta ccgtttctcg
    27721 ggttatcaat aaaaaaggat atctatctga gaaaaccatc caaaaagtca atgaagccat
    27781 gagagaatta ggctataaac ccaacaacct ggctcgtagt ctgcaaggaa aatcagctaa
    27841 gttaatcggc ttgattttcc ccaatatttc caatgttttc tatgcagaat tgattgataa
    27901 attggaacac caactcttca aaaatggtta caagaccatc atctgcaaca gtgaacatga
    27961 ttctgagaag gaacgcgaat acatcgaaat gttggaagcc aatcaggtag acggcatcat
    28021 ttctggtagt cacaacctag gaatcgaaga ctacaatcgt gtgacagcgc cgattatttc
    28081 ctttgaccga aacctatcgc cagacatccc tgttgtctcc tctgacaact atgctggtgg
    28141 ggttcttgct gcccaaacct tggtcaagac aggtgcccag tctatcatca tgattacagg
    28201 gaatgacaat tccaattcgc caaccggact gcgccacgct ggttttgcat ccgtactccc
    28261 aaaagctcct attatcaatg tttccagtga cttttctccc gtcagaaaag aaatggaaat
    28321 caagaatatc ttgacccggg aaaaaccaga tgccattttt gcttcggatg atttgacagc
    28381 tattctggtc attaaaatcg ctcaagaatt gggcatttct gtcccaaaag agctcaaggt
    28441 catcggctat gatgggacct actttatcga aaattactac cctcaattgg ctactatcaa
    28501 gcaacctttg gaagagattg cttgtctcac tattgatctt ctcttgcaaa agattgaagg
    28561 caaggaagtc gccacaactg gttacttctt accagttacg ctattaccag gaaaaagtat
    28621 ttaaacacaa gaaaactcag accgattcgt ctgagttttt atgatcttaa attttcgaga
    28681 tagcgctggg ctgtctctag gttaaaggtt ttatctgaga tgaggcgctc tactagggga
    28741 gcaacttcag attcactagc cccagctagg agagctaggg atttggcctg tagtttcatg
    28801 tggccttgct ggatgcccgt acttaccaag gctttgaggg ctgcaaaatt ttgagcaaga
    28861 ccgatggaca cgataatctg ggctaattct ctggcagaag gatttcctag tagatcatga
    28921 ctgagagcta cacgtgggtt gaggccgata gagccaccct tagtcgctac aggcatgggc
    28981 agggtcatct caccgaccaa ttcttctctt tcaaggtcca gcgtccagca gctaagacct
    29041 tgatagcgtc catctcgact ggcaaaggca tgggccccag cttcgatggc acgccagtca
    29101 ttaccagtgg caatcaaaat cgcatcaata ccattaaaaa ttcctttatt atgagtagca
    29161 gctcggtaag gatcagcctg cgcaaactga ctagccaacg caattttctc cgcaatctct
    29221 cgtccttgat ccttttggcg gctcaagtag cgaaaggcga tgcgacagct tgcagtcacc
    29281 agagaatcag tcgcgtagtt ggacaggatt cccatgagac tctgtccctg actgagttct
    29341 tctaagactg gtttcaaggc ttccagcatg gtgttgagca tattggcacc catggcttcc
    29401 tgggtatcga catgaatata aacaacgaga aagtctggtt cgccttttat ctgctcgaca
    29461 tgcagatcac gcgccccacc cccacgttta acgatagaag gataggcttg attggcaagc
    29521 tccaagagct ccgctttctt gctggcaatc ttctcttgcg ctagtttagg attagcaact
    29581 tgataaaggg ctacctgccc aatcatctgt cgctgatgga cttgtgcagt aaaaccacct
    29641 gcacgcttga tgattttgct ggcatagctg gccgccgcaa ccacagaggg ttcttctgtc
    29701 acatagggaa cggtgtattc ctgaccgttg acaagtacct ccggaaccag tgaataaggc
    29761 agagaaaaag ttcccactac attctcactc agctggtctg ccacagtcac gctcatctgt
    29821 tcatccttct ccagactagc ttgtctctca ggactaagga gcgcctgagc ttttaacagc
    29881 tcgaggcgct cttggtatga ttttttagaa aatccattcc aacttatctt cattattttt
    29941 caaccttgct ataacggcgt tggtggtcga gaatttcaac caaggcaaaa tcttgatttt
    30001 catagccagc aaactgggca gagtttgttt catccaagtt tacttcctca aaaaagacct
    30061 tttcatagtc tgcaacggat agggcagttc gttggttgag cttgttcaag cggtctttat
    30121 ccaaataagc ttcatatcct tcaaccaatt caccactgaa gaactcagcc acagctccac
    30181 ttccgtaact ataaagggcg attttatccc cagctttcaa gctatctgta ttttccaaga
    30241 gagacaaaag tccaaggaaa agtgaacctg tgtagatatt ccccaccttt tgactgtaga
    30301 gaatagactg gtcaaaatgc ttttgtaaga ggtctttttt ctcttgaggc aggctcttat
    30361 ccatgatttt tttcaagcct tttagcgcta atttaggata aggcaagtgg aaacaaacag
    30421 ccgcaaaatc atccagagta agctggtagc gtttttgata ttcaagccaa gtcgttttca
    30481 aactatccaa gtattgttgg gtagaataaa caccatttac ataaggagtt gtcgagtaat
    30541 ttggtcgcca gaaatccatg atgtcacggg tctgagctac attgtcatta ttaaaggcca
    30601 tcatgcgtgg attttgtgta atcaacatag ctacacttcc agcaccttga gttggttctc
    30661 ctggagtttc aataccgtat ttggcaatat cactggcaat gaccaagacc ttggactccg
    30721 gagaattttc cacatgcaat ttggcataat gaagggcagc agtcgctcca taacaggctt
    30781 ctttaatctc gaaactacga gcaaagggct ggatgcccag caagccatgc acaaagacgg
    30841 ccgcagcctt actctggtca attcctgact cggtagccac aatgaccatg tcaacttctt
    30901 gtctttcttg ctcagttaaa atagagtcac tagcactggc cgccaaggtc acaatgtcct
    30961 cagttagggg cgcaatactc aattccttga gtaagagtcc tttacttaat ttttcagggt
    31021 caattcccct cgcttctgct aagtcttgta atttcaagac atattgactg gtcgcaaaac
    31081 caatcttatc aataccgatt gtcatattta cctctgtttt atcattcatg taaaaaatcg
    31141 ttctatacta ttttatcaca aatggcagta aaagagagaa aaaagacttg attcaccaaa
    31201 tcaagcctct tattggtcat cattttaaag aatgattagt tgctagaaag ttcaccgata
    31261 taagtagctt tataagcttc attcacagtt atcagctcct ggaggatcaa atttcctgag
    31321 taagtccttc ccatctcatc tacaaattct tgataaaact gactggtcgg aatttctctg
    31381 acatccttat caaatgtctt gtcaagtgtt ttactaacct tctcagcaat caattgatgc
    31441 tcttaccatc cactttgaaa ctctgagccc gaactagaaa ccatgactgg gataaacaac
    31501 aaggtcagta gatttacaga caataaggaa agtagtagac ttcctgcaaa actagaatcc
    31561 tagttcatga ttgataatgc cagcaatcaa attcattcat aatccgaagc gtttacgatg
    31621 atttcaatag gttgttgaaa acattttaaa cgtttttact ttggcaaaga tgttctcaac
    31681 cttgcttctc tccttggata gcgcatggtt ataggcttga tcttcaactg ttagcggttt
    31741 gagtttgctg gatttacgtg gagtttgtgc ttgaggatat atcttcatga gcccttgata
    31801 attactgtca gccaagattt taccagcttg tccaatattt ccgcgactca ttttgaacaa
    31861 cttcatatca tgacaatagt tcacagcgat atccaaagaa acaattctcc cttgacttgt
    31921 gacaatcgct tgaggttcca tagaatgaca tttctatttt accagaataa ttggctagtt
    31981 gattttttta ggacgattta tttttacctc tgttgcatcc acaatcaccg tattctcagc
    32041 actaagatga gtttttgaaa tcgtaacacc actttgaaca agagttactt caacccattg
    32101 gctccgacgg attaagttgc tttcgtggat accaaaatca gccgcaattt cttcataagt
    32161 gcggtattct cgcacatata gaaagcgttg ttaatttatt tatctcattt ttcagaaaat
    32221 tcttttattt ctgtaaagtc tacgatactc gatgtgtttt tatataatga tacagtctga
    32281 gaatcactgt tccactagcc attccaatag agattaccaa agccaacatg acaaccaagg
    32341 tcgcacttgc cagtgcttta ttatagtccc ctgtcacaaa aaaggcagtt gttcggtagg
    32401 agagataacc tggaaccagc ggtgccaaaa tggccaagat aaagaccaca gcaggtgtct
    32461 tataaagaat acttaaaatc tggctgacac aagaaccaat aatggctgca atgaaggtag
    32521 ctacaatgac attggtcggt tccttgagca agagatagat tagccagaca gtcatgccca
    32581 aaatccctcc aggtaagagc atagaccgtt gcacattgag tacgattaaa aaagtgataa
    32641 tggcaagaaa acttgctact gcttgtaata aaaaggttgt tagtgtcata ttagttcatc
    32701 aataccaagg cgacagaagt tcctgcccct aaagcgaggg taatgagcag ggattcaaac
    32761 atcttactca taccagagtt tatgtggttg gtcataatat cacggaccgc attggtcaag
    32821 gcaatacctg gtacaaacgg catgaccgca ccagctataa tcaaatctgc cgttgaagga
    32881 aaacctgtgt agcgagccca aaactgggca attatcccaa agacaaaggc tccagcaaag
    32941 gctgtcacaa agggaattcg gataaatttt tccacataga gggaaaaggc aaaaccaaat
    33001 aaggtcgcca ctcctgcccc aagtgcgtcg tagatatttc cgctaaacat aactgaaaag
    33061 aaaggagcgc taaaggtcgc agccagagtt acctgcaact tagtataggg aaggggttga
    33121 gcttgcaagg ccgtcaattg cttaaaggct gtttctaagt caatctgccc cccaactagc
    33181 tgacgagaaa tctggttcac atcgcagact ttttcgatgt tataagaaga ggaggtcacg
    33241 cgcttcatgc gcgaaatatt ggtattttca atagagaaaa agatagcggc aggcatggca
    33301 aggacattgc aatccacaat cccctgcgaa tgcgcgattc gaatcatggt atcttctaca
    33361 cgatggattt ctgagccact tttaaggaga atagtccccg ctagcataat cacatcaatg
    33421 acggcattta attcttttga ttcttccatg ctttcctcct tttatcaact ccctctattc
    33481 tatcacaaat ctggactcaa aaaaaatctt tgccatgaaa tcatgacaaa gattgattac
    33541 tcattttgat tatccatctg cttttaagga gtagctgaag ttgttttagg tttgtagatt
    33601 gaaatcttga ctctagtctt atttaggtct accttttcac ctgctctagg actttgttca
    33661 acaaccatgc cttctacact acctgcaggc gctgtcgtca cttctacaac ttctatatta
    33721 gcttccttaa tcccaacaat ttgaatcaaa ttgttcttag taaactccaa gctggaacca
    33781 atgtaactcg gcatggcaac acttgtaact tttttagcta ctgtcaagac aatttgagta
    33841 ggtttactca catcataagt cgttccggca cctggacttt gtttcataat cgttcctggt
    33901 tcgctttcgc tggactcttc ttcctctatc ttaatcaaat tctcaggaac cttcttctgc
    33961 ttgagttctg agattacttc tgtagaattc cgtccaatat agttccctaa ttgaatcgtc
    34021 gtagcttttt tagctactgt caaaacaatt tgagttgcct tgctcaagtc ataggtcgta
    34081 ccttctggta gactttgctt caggaccgtt ccagcctcac tctcattcga ctcttcttcc
    34141 tcaattttaa tcaaattatc tggaactttt ttctctttta attccgcgat aacatcagaa
    34201 gatttccggc cgacataatt actaatttgg aaggattgtt tgcctgatga gacaaccaga
    34261 ttgattttcg ttccttcttt tcgaccagtt ccagcgccag gatctgtacg gataatccgc
    34321 ccttcttcca ccttttcact agcctctgtc ttctcctcac caatctcaaa attggctttt
    34381 ttgagcgttg ccttggcctc tgcaactgtc tgacctgcca catctggaat ggcaatggtt
    34441 gcaggagttc tggatagtat ccaaataaga gaagctgcca ccaatacaag gctggccaac
    34501 aaaatcaggt aacgcatctt aaatctatgt tttttcggtg cttgtggttg gtaagtttcc
    34561 tctgtcacag cctggcttgg gtttttgatt gatttgtgtt ctgtctgcgc ttgaacctta
    34621 ggaatagatg tcaaggtact ctgagaaacc ttcggcaagg tcttggtatc tgccttgctc
    34681 gtttcatcaa agattaactt actttcattt ctacgattgt aggacaagct actagacaag
    34741 tccacataca tctctgaaac cgagcggtag cgattggtca actttttagc agttgccttg
    34801 ataataacat tttctaaagc ctgaggtaca gatggatttt ctgcaataac ggacggcagg
    34861 ggtttctgga aatgctggag ggcaatggtc accgcgctat ccccgtcata agggatatgg
    34921 cctgtcaaca tctcatagaa aataatcccc atggcataga tatcactctg cacagtcgcc
    34981 ttcgaaccac gcgcctgctc tggtgacaag taatgaactg agcccaacat cgagttagtc
    35041 tgggtcagac ttgtctctgc aaaggctaca gcaatcccaa agtctgtgac cttggccgtc
    35101 ccatctggtg tcaaaaggat attttgaggt ttcaagtccc tgtgaacaat tcctcgagta
    35161 tgggccaagc gcatagccaa gagaatttgt cccatgatac ggactgcttc ttcattagaa
    35221 agaggataat gttccttgat atagcgtttg aggtccagtc cagccacata ctccatagct
    35281 aggtactgtt gaccgtcttc ctcgccaata tctgttatcc gaacgatatg aggatggtct
    35341 agatctgcca tagctctcgc ttcacgctga aaacgagcta cagctatcgg gtccgtctgg
    35401 tagttggtcc tcagaacctt cactgccact tcttccccat ctaagattaa gtctttggct
    35461 aggtagacat ccgccatgcc tcctcgacca atctgtttga caatccgata gcgtccggca
    35521 aaaatcttgc cgatttggat cattctgcat cctcctcgtt catagaaaca agggcaaccg
    35581 taatgttgtc taaacctcct gcattgttag caaaacgaac aagtgtctcc gttttatctg
    35641 ctaaaggaat atcactggtt acaatatcac gaatctcact gcctgaaatc atgttggtca
    35701 agccgtcact attgagcaag agatagtcac ctgactcaag gataactgtc ccaaaatcag
    35761 gctgaatttc atctttttgc ccaatagact gggtgataat atttttttgc ggatgagctt
    35821 ctgcctcttc tggtgtcaat tgaccagcct tgagcaattc attaaccaag gaatgatcgc
    35881 tcgtcaactg atggtattct tctccacgaa tcaagccgat acgcgaatca ccaatatgag
    35941 catagatagc ctgattatca ataatagcaa ggacttccaa agtagttccc atgcctctgt
    36001 aagcttcatc ctgaccaagc tggtgaatct tttgattttc aatttctagg taatgggcga
    36061 accattcacg cacttcattg actgtatcga tctgggtatc aacccaagct acacccaggt
    36121 ctgtgaccgc catttcacta gcgatattcc ctgcacgatg acctcccatc ccatcagcta
    36181 aaataatcat ggtacgtcca gctctattga catagtggtt gacatagtct tggttatttg
    36241 ttcgtttctg accaacatct gttaataatg aaatttccat gtgtcagttc cttcctaatc
    36301 cgatatcttg cgaaattgac tgatgaagaa tccatcactt ccatacaatt caggtgtaat
    36361 gaggatacag ccgtctttca tgatatcctt acattcatgt tctagtttta cctgctcgaa
    36421 ctcgggatga ctctctaaaa aggccttaac gacttgaaaa ttctcctctg agacgatagt
    36481 gcaggtgcta taagttatta taccaccttt gcctagtatt tgacaaacac tacctaatat
    36541 ttctaactga atttcctgca aggacgcgaa atctgccgtt tctttattgt atttgatatc
    36601 tggttttcgg cgcaaaagac cgattcctga acaaggagca tccaccaaaa tcttatcaaa
    36661 ggaatcctgg tcaaaaaact catgcacctt tctggcatcc aatttttgag tttgaaccca
    36721 atcttcaact cccagacgtt gggcattttc ttgaattaaa tccaacttgt ggtcgtacaa
    36781 gtccagagca gtaacctgac ctgtcgtaag ataagaggct atatgggctg ttttcccacc
    36841 tggagccgca caggcatcaa gcactcgctc atcaccttgt aaatcaagcg tcggagcaac
    36901 caactgactg gactcgtctt ggatggtaat ggctccatcc gcaaacaaat tatgccctgc
    36961 aaaatgcccc tgctccttaa ccagaccagt ggttgctaaa agggaattat tcgcctccaa
    37021 caaggcttgg atttcctctt ttcgacttag gtctgttaca cgaatactgg ctttgtttcg
    37081 cactaacagg ctttcaaaga tggcttttgc tctctcctct ccgtattctt ccttgagttt
    37141 ggcaactagc caaactggga gagaataggc aatggagtca cgcttgtttt ttcgcttgat
    37201 gctagcaata tctggccagc cttcacgcaa gatacggcga aggacagcgt tgaccaattt
    37261 ttcactgcct tttttacgga gtttggccaa ttccactgct tcattgacca cagcatgatc
    37321 tggaatcttg tccaaatagc ggagttggta ggcactcatg agaagaagga catagagcca
    37381 gctgtctaac tggtctctgt cttcgataaa gtgggatagg taccattcca gagtcagttt
    37441 acgggctacc gttccataga ccagctcggt cactaagccc ttgtctgctg ccaaaagttg
    37501 acttcccttt agatgcttat ttaaggcgat atttgagtat gcttggttca caaaaacatc
    37561 ctctagcact gctagagcta aacttctagc cgtttctact ttagtcacca aatcgttctc
    37621 ctacagtcaa tgtacgtcca actccgttga ggaaggaagc aatgtccatc ttaggcttac
    37681 cagctggctg cacttgtttg agggatagag ccccttcagc cgttgcgaca atcaattctt
    37741 tcttgccgat agagagaatc tcacctggat ttccctgacc ttctactggt agggcttcat
    37801 aaatcttaaa gcggtcgccc ttaaggaaag tatgggcaac aggccagggg ttcattccac
    37861 gaatttggtt aaagagttga cgattggttt tgttccagtc cagtttttct tcctctggct
    37921 ttatatttgg agagaaggta acctgactcg tatcctgcgg ttcaggtttg atatcaccag
    37981 caatataggc aggcagagtg tccaaaagca aatcacgacc aactagcgcc aatttttcaa
    38041 acaaggtgcc aacattgtcc tcatctgtga tcggaatgct gcgacgagaa atcatatctc
    38101 ctgcatccat ttccttaacc atttccatga tggtcacacc agcttcctca tccccttgaa
    38161 tcaaggcata atggataggc gcaccaccac ggtgtctagg aaggagggag gcatgaacgt
    38221 tgacagcaaa gtccatgcta tcaaggagtt tgcttgggag aaactgccca aaagcagcag
    38281 tcacaattcc atctgctcct agcttcataa gatcttccat ctctggactt ccagataatt
    38341 tttcaggttg gtagatagat aatcctgctt ccttggcagc ctgcttgact ggggtttctt
    38401 ggataacttt tttacgacca acagcacggt ctggctgggt cacaacggct agaatttcgt
    38461 aacggtcatc tgtcaaaagt ccttttaaga ctgttgctga aaagtcgggg gtccccataa
    38521 agattagttt tgtcatatct tctccttctt ataaaaattg ctgcggctca tggtcaatgc
    38581 tgagacggag ctcactattt tcccgttctt gagtcaaggc caagacctgg ttgagggtcg
    38641 gccccagctc atcttctaaa cggtatttaa ttaaaatctg gtaatgatag aggttgtggg
    38701 tacgggcaat cggttttggc gttggcccca gaatgggact ggtctctgac aagcctgacc
    38761 gcaaaatgtt catgacttca taggcacgtt tgaaaacctc ttcttctttc ttgtgagaaa
    38821 gggtaatacc aatcgtgaaa tagtaaggtg gatagccgag ttgtcgtctg attcccattt
    38881 cataggcata aaagccttcg taatcttgat ccttggcaaa tcgaatagca tagtgctgcg
    38941 aattgtagga ctgtatcaag acttgacctg ctttttcagc ccgacctgct cgacctgcca
    39001 cctgagtcaa gagctggaag gttctctcag aagaacggaa atcaggcaga ttcaaggccg
    39061 tatccgcatt tagaactccg actagggtaa cattgggaaa atccaaaccc tttgcaatca
    39121 tctgagtacc aagtaaaata tccgcttccc ctcgcccaaa ctggtcaagc aaggcttggt
    39181 gactgccttt ctttcgagtc gtatccacat ccatcctcaa aatgcgagct tggggaaaga
    39241 gttctgctag ctcatcataa gccttctgag ttcccgtccc atagtaacga atactgcggc
    39301 tcttacagtt aggacagacc tgaggaatat ccttcgagaa accacaataa tggcagttca
    39361 tagtcttggt atccatatgc aaggtcagag aaatatcgca gttgggacaa gtatccaccg
    39421 tcccacactc ccgacacatg acaaagctag aataaccacg acgattgagc atgagaacca
    39481 cctgctcttt tttaaccaga cggtcttgga tagcctctag caaaggaggc gtaaagtttg
    39541 acgtctcatt ttgtccgata tagtctcgaa agtcaatcac ttgaacctca gggattgtag
    39601 ccaaaggatt ggcacgttgg gttagacgta agtgttgata gacgcctttg ccagcacgcg
    39661 cccggctctc taagctcggc gttgcagatc caagtaccag agttgcttga ttatactgag
    39721 cccgtaaaat agctacctct ctggcatggt aacggggatt gctgtcctgc ttataagcca
    39781 cttcatgctc ttcatcaata atcatgacac ccagattttt cagaggagca aagatagcag
    39841 atctggcacc aacaacaact tgggcatcgc cacgctccac cttgcgccat tcatcatact
    39901 tttcaccatt ggataatcct gagtgaagaa tggctacctt gtccccaaaa cgtgctataa
    39961 aacgctcggt catctgagga gtcaaggaaa tctcaggtac cagcaaaata gctgtcttgc
    40021 ccttatccag ggcaccttgg ataatctgca agtaaacctc ggtcttccca cttcctgtaa
    40081 tcccttgaag tagaaaggga ggttgagaac tgccaataga actcacaacc gcatcacgcg
    40141 cctgtctttg ttctggattt aactccaaag gtctacttgc ttcaattcct tcaaaataag
    40201 cagccgagcg ttgaacttcc ttttggacta tggtaacagc accttgatcc acaaagaagt
    40261 tgacttgctc tcgcgagtag gactctaaca agctagccaa ggaagcgctc tctggatgag
    40321 acagcagata atctctcagt tccaactttt tcttggcacg tgtagaaatc tcaacacctt
    40381 ctaattgagc atggtcaacc tcataccaag actgggtctt gaccttcttt tgatcgactg
    40441 cctgatattc cagaccaagc aggccttttc tagtcaaacg catcatttca gcttgcttgg
    40501 caaggtctag tgaagaaaag gctagcgaat cttctgaacc aaacaggcgc actcgttctt
    40561 cctgactcaa gccttccaga ggatagagaa tcttgtcata gctagaattc agaaatcctg
    40621 gaagcatggc cttgaggata gagattttgt aggagaagac agatttgcgt aactcctcag
    40681 ccagccagag ttgttctggc gtgagaacag gagaaaaatc cagcacctct gcaatatctt
    40741 ttaaatcttg ctccatctct tctccatctg attgggactc caaaccaaga acaatccctt
    40801 gaatcaggcg attaccctta ccaaaaggca catgaacccg catcccaact tccagcattc
    40861 cctcaaattc ctccggaatc ctgtaactat agggctggtc cgtctgcatc aagggcacat
    40921 ctacgataat cttagctagg gccatcttct cacctcctcc ttgtcagtac attcttgcaa
    40981 tagaaaaaat aagattgagt ccccccaacc ttaaattttt tcaccatctt ctttttcttt
    41041 agcaatttgc tctttgattt tcttttcttc ttcttctttg cggcgttttt cttcttcgat
    41101 acggcgacgc actgcctcac gttttccttc tggatctggg tgaattgtaa cgtttcctga
    41161 ttcgatttct tctaaagcgc gaagagttga tttttcagac ttgaaacctt gagttgctgg
    41221 ggcacctgct tccaattcgt gggcacgttt tgcttccaag attacgagtg aatattttga
    41281 aggaaccttg tcgagcaagg tatcaataga gggttttaac atcatttgct tgtacctatt
    41341 ttctaaattt tatcgggtag ttggagattt tggtaacatc tcctgatagt gaccaatgac
    41401 acgatccaca cagaagtgtt ctgcttcaat cacacatttg acacgttcag cagctagggg
    41461 cacctgatcg ttgacaatcg cataatcata ctcacgcatg agggcaattt cttccttggc
    41521 cttttcgatt cgttgggcaa tcacttctgc actatctgtt ccacgaccta ccaagcgatc
    41581 ttgcaattca tccaaatctg gtggtgtcag gaagataaag acagcatctg gaaccttttt
    41641 cttgacctga agagcaccct gaacttcaat ttcaaggaaa acatcgattc ccttgtccaa
    41701 ggtttcattg acataggtca gaggagttcc atagtagttg ccgacatatt ctgcgtattc
    41761 caacatctgt ccttgacgaa tcagctcttc aaattcttca cgagtacgga agaaatagtc
    41821 aacaccgtcc acttctccag gacgttgtgc gcgtgtcgtc atcgatacag aatattgaaa
    41881 ttggttttca gaactctcaa aaatctctct tctaaccgtt ccttttccaa cccctgaagg
    41941 accagaaaaa acgattagta agcctcggtc tgccattgtg tctcctttta gtcaatctgt
    42001 gaaataacat ttctctagaa taatggcaaa aagccagatt atcctttaca gtctttctat
    42061 ctagtgtaac aaaaaagcag taatttttca actgctcttt cttatttatt tagcataatc
    42121 tactgcacga agctcgcgaa tcacggttac cttgatattt cctggataat cgagattgtt
    42181 ttcaattttc ttacgaactt tgtgagccaa gattgtgact ttgtcgtcct tgatttttcc
    42241 tggattgacc atgatacgaa tttcacgtcc tgcttgaagg gcaaagctag tttgcactcc
    42301 ttcaaagccg ttagcaattt cttccaaatc atggagacgc ttgatgtagc tttcaagaga
    42361 ctcactacga gcacctggac gggctgcgct caaggcatct gctgcagcga cgataactgc
    42421 tatcacgctc tcagcttcaa catctccgtg gtgactagca atcgtattca ccacaactgg
    42481 gggttccttg tacttacggg ccaattccat accgatttca acgtggctac cttcaacctc
    42541 atggtcaatg gctttcccga tatcgtgaag gaatccagca cgacgggcaa gagccgcatt
    42601 ttcaccaagt tcgctcgcca tgataccagc caacttagca acctcaatcg aatggcgcaa
    42661 aacattttgt ccatatgaag tacggaactg caaacgtccc ataatcttca tcaagtctgg
    42721 atgaaggttt ggcgcaccaa tttcataggc agcagcctca ccgtattcac gaatcttatt
    42781 gtcaatctct tgacggtttt tctcaaccaa ctcttcgata cgagctggat gtatacgacc
    42841 atctttgagc aacatttcga tagtcatacg ggcaatctca cgacgaatcg gatcaaatcc
    42901 tgacaaggtc accacttctg gtgtatcgtc gataatcaca tcgacccctg tcaaactttc
    42961 aaaggtacga atgttacgac cttcacgacc aataatgcgt cccttcatag tatcgtctgg
    43021 cagatgaact gttgagtttg ttgactccgc tacatattca ccagcgatac gttgcatagc
    43081 ttgaaccaag atgtccttgg ccattttgtc agaacgttcc ttgacctctt gctcagcttc
    43141 gcgaatgcga ctggcaatct ccctggtcaa gttttcctct gtctgagcca agataatatc
    43201 tcgtgcttct gcctgagaca gagcaccaat acgctctagt tctgcttctt tttgtctttc
    43261 gacttcctct aattgctctt cacgcgcatc aaggtttttc gctctatcag aaatactttg
    43321 ttctttttgt tcaagtgttt gttctttact cgtcaaattg tcgtccttac ggtcaaggct
    43381 agtagctctc tctgtcaaac gactttcgat ttgtttgagt tcttgacgtt ctgatttgaa
    43441 ttcagcgtcc acttcttcac ggtattttct ggcttcttct ttggcctcca atagtgcttc
    43501 ttttttaaga gacttgcttt cacgtttggc ttcattaaca agtaaatccg cttcacgctc
    43561 agcttgtcca cgtaaattag ttgcttcttg ttcagcattt aaaagcatca actctgcagc
    43621 ttcctgagat gatttcatct tagctgagat gctgacatat ccaatgacta aaccaatgat
    43681 gacggcaaaa acagcaatcg caagcgacat gatttccatg tttttacctc attttattgt
    43741 tattccgaat gacatacatt cttttacatt ctaccataaa aaagtgattt tcacaaacct
    43801 aaaatagaat atgttttggg ga
//
LOCUS       DAVDWT010000017        43507 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVDWT010000017 DAVDWT010000000
VERSION     DAVDWT010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43507
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.017"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            237..518
                     /locus_tag="VUW50_001058"
     CDS             237..518
                     /locus_tag="VUW50_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET7982966.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYQDGVDLLGYTTWS
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            650..1594
                     /gene="manA"
                     /locus_tag="VUW50_001059"
     CDS             650..1594
                     /gene="manA"
                     /locus_tag="VUW50_001059"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HET7982967.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGSHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(1634..2969)
                     /locus_tag="VUW50_001060"
                     /pseudo
     CDS             complement(1634..2969)
                     /locus_tag="VUW50_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            3197..3937
                     /locus_tag="VUW50_001061"
     CDS             3197..3937
                     /locus_tag="VUW50_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7982968.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSETAQYTSDAIAAYVQTNAE"
     gene            complement(3966..4421)
                     /locus_tag="VUW50_001062"
     CDS             complement(3966..4421)
                     /locus_tag="VUW50_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET7982969.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4441..5616)
                     /locus_tag="VUW50_001063"
     CDS             complement(4441..5616)
                     /locus_tag="VUW50_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7982970.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITELEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5675..6520)
                     /locus_tag="VUW50_001064"
     CDS             complement(5675..6520)
                     /locus_tag="VUW50_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET7982971.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEDGGLLM
                     GYEID"
     gene            6645..7202
                     /locus_tag="VUW50_001065"
     CDS             6645..7202
                     /locus_tag="VUW50_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7982972.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7221..7688
                     /locus_tag="VUW50_001066"
     CDS             7221..7688
                     /locus_tag="VUW50_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HET7982973.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7777..8406
                     /gene="upp"
                     /locus_tag="VUW50_001067"
     CDS             7777..8406
                     /gene="upp"
                     /locus_tag="VUW50_001067"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET7982974.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFIVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8580..9170
                     /gene="clpP"
                     /locus_tag="VUW50_001068"
     CDS             8580..9170
                     /gene="clpP"
                     /locus_tag="VUW50_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HET7982975.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9249..9497
                     /locus_tag="VUW50_001069"
     CDS             9249..9497
                     /locus_tag="VUW50_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HET7982976.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9598..10758
                     /locus_tag="VUW50_001070"
     CDS             9598..10758
                     /locus_tag="VUW50_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7982977.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVIDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGRDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            11026..11895
                     /locus_tag="VUW50_001071"
     CDS             11026..11895
                     /locus_tag="VUW50_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7982978.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGAISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFEMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            11899..12855
                     /locus_tag="VUW50_001072"
     CDS             11899..12855
                     /locus_tag="VUW50_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7982979.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            12855..13619
                     /locus_tag="VUW50_001073"
     CDS             12855..13619
                     /locus_tag="VUW50_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982980.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKDKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13619..14329
                     /locus_tag="VUW50_001074"
     CDS             13619..14329
                     /locus_tag="VUW50_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7982981.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14688..15344
                     /locus_tag="VUW50_001075"
     CDS             14688..15344
                     /locus_tag="VUW50_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7982982.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQELHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            15452..16547
                     /gene="prfB"
                     /locus_tag="VUW50_001076"
     CDS             join(15452..15523,15525..16547)
                     /gene="prfB"
                     /locus_tag="VUW50_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET7982983.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16565..17257
                     /gene="ftsE"
                     /locus_tag="VUW50_001077"
     CDS             16565..17257
                     /gene="ftsE"
                     /locus_tag="VUW50_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET7982984.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17250..18176
                     /locus_tag="VUW50_001078"
     CDS             17250..18176
                     /locus_tag="VUW50_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7982985.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            18462..20642
                     /locus_tag="VUW50_001079"
     CDS             18462..20642
                     /locus_tag="VUW50_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET7982986.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            20694..21509
                     /locus_tag="VUW50_001080"
     CDS             20694..21509
                     /locus_tag="VUW50_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET7982987.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYNRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            21649..22992
                     /locus_tag="VUW50_001081"
     CDS             21649..22992
                     /locus_tag="VUW50_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7982988.1"
                     /translation="MSFTKFQFKNYIREALEELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            23207..24397
                     /locus_tag="VUW50_001082"
     CDS             23207..24397
                     /locus_tag="VUW50_001082"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET7982989.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            24950..25885
                     /locus_tag="VUW50_001083"
     CDS             24950..25885
                     /locus_tag="VUW50_001083"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HET7982990.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            25919..26956
                     /locus_tag="VUW50_001084"
     CDS             25919..26956
                     /locus_tag="VUW50_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET7982991.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKVDSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            27129..27734
                     /locus_tag="VUW50_001085"
     CDS             27129..27734
                     /locus_tag="VUW50_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET7982992.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            27891..28421
                     /locus_tag="VUW50_001086"
     CDS             27891..28421
                     /locus_tag="VUW50_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HET7982993.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            28442..29527
                     /gene="rlmN"
                     /locus_tag="VUW50_001087"
     CDS             28442..29527
                     /gene="rlmN"
                     /locus_tag="VUW50_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET7982994.1"
                     /translation="MKPSIHSLPHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFVCTINDDKGMAIGARHITISTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            29527..30009
                     /locus_tag="VUW50_001088"
     CDS             29527..30009
                     /locus_tag="VUW50_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET7982995.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKVRN
                     "
     gene            30011..31552
                     /locus_tag="VUW50_001089"
     CDS             30011..31552
                     /locus_tag="VUW50_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET7982996.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     SLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(31608..32411)
                     /locus_tag="VUW50_001090"
     CDS             complement(31608..32411)
                     /locus_tag="VUW50_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7982997.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(32494..32724)
                     /locus_tag="VUW50_001091"
     CDS             complement(32494..32724)
                     /locus_tag="VUW50_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982998.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEEVEDFQDDDEIPTII"
     gene            complement(33203..33400)
                     /locus_tag="VUW50_001092"
     CDS             complement(33203..33400)
                     /locus_tag="VUW50_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7982999.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKRNMDLLADD
                     SLAGDIILLWRIQFGTFTTET"
     gene            33570..33842
                     /gene="rpsP"
                     /locus_tag="VUW50_001093"
     CDS             33570..33842
                     /gene="rpsP"
                     /locus_tag="VUW50_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET7983000.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            33862..34101
                     /locus_tag="VUW50_001094"
     CDS             33862..34101
                     /locus_tag="VUW50_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET7983001.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            34678..35466
                     /locus_tag="VUW50_001095"
     CDS             34678..35466
                     /locus_tag="VUW50_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7983002.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELLE"
     gene            35497..36015
                     /gene="rimM"
                     /locus_tag="VUW50_001096"
     CDS             35497..36015
                     /gene="rimM"
                     /locus_tag="VUW50_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET7983003.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            36005..36724
                     /gene="trmD"
                     /locus_tag="VUW50_001097"
     CDS             36005..36724
                     /gene="trmD"
                     /locus_tag="VUW50_001097"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET7983004.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRVQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            36736..37074
                     /locus_tag="VUW50_001098"
     CDS             36736..37074
                     /locus_tag="VUW50_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983005.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(37105..37425)
                     /locus_tag="VUW50_001099"
     CDS             complement(37105..37425)
                     /locus_tag="VUW50_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983006.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            37523..37738
                     /locus_tag="VUW50_001100"
     CDS             37523..37738
                     /locus_tag="VUW50_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HET7983007.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(37852..37938)
                     /locus_tag="VUW50_001101"
                     /pseudo
     CDS             complement(37852..37938)
                     /locus_tag="VUW50_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(37989..38525)
                     /locus_tag="VUW50_001102"
     CDS             complement(37989..38525)
                     /locus_tag="VUW50_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HET7983008.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            complement(38722..40068)
                     /gene="gor"
                     /locus_tag="VUW50_001103"
     CDS             complement(38722..40068)
                     /gene="gor"
                     /locus_tag="VUW50_001103"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HET7983009.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNRESYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAASEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTSSEEFVTMR"
     gene            40333..41532
                     /locus_tag="VUW50_001104"
     CDS             40333..41532
                     /locus_tag="VUW50_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7983010.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PIHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVARADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            41516..42217
                     /locus_tag="VUW50_001105"
     CDS             41516..42217
                     /locus_tag="VUW50_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7983011.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            42219..43478
                     /locus_tag="VUW50_001106"
     CDS             42219..43478
                     /locus_tag="VUW50_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7983012.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESL
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
BASE COUNT        13322 a         8037 c         9476 g        12672 t
ORIGIN      
        1 attgaaacta gaatagtaca cctctacttc taaaacattg ttagaaatcg atttgtcctg
       61 ttcttgtttc attttactat agaacgattt gaaggcgttt ataatattta gctgtacgag
      121 agtcttttaa aagtgttttg atggtttgga tttcttcttt agttgatttc atattactat
      181 tgtataatgc tttttgattt tagtttagta ttatcgtaga aaatggactc ggtgctatgg
      241 atacaccgga tgagaatggt tatgtagcag atgactatcg gattacttac ttagaggccc
      301 acatcaaggc catgcgagat gccatttacc aagatggggt tgacttgctt ggttatacga
      361 cttggagctg tatcgatccg gtttcagctg gcacagggga aatgaataag cgctatggct
      421 ttatctatgt agatcgagat aatgtaggaa atgggactct caaacgtagc aagaagaagt
      481 ccttctattg gtacatgtca tttatagcaa tggtgtaagc atcgagtaga gcacatttgt
      541 ttactatttt tataaaatct tctccctgct ttataagatg taaaaaagag ttcaaatgga
      601 actctttttt tgctataatg aggggagaaa aatcagacag gagattaaga tgtcagaacc
      661 attattttta caatcagtta tgcaagaaaa aatctggggt ggtaccaagt tacgtgatga
      721 gtttggctac gacatcccaa gtgaaaaagt cggagagtac tgggctatct cagctcatct
      781 aaatggtgtt tctaaagttg ccaatggtcg ttacgaggga acagatcttg ctactttgta
      841 tgcggaacac cgtgaattat ttggcaatcg tccagaacct gtatttccgc ttttgaccaa
      901 gatcctcgat gccaacgact ggctcagtgt ccaagttcac ccagacgatg cttatggact
      961 cgagcatgaa ggcgaacttg gaaaaacaga atgttggtac atcattgcag cagatgaagg
     1021 ttcagagatt atctacggtc acaatgccaa gtcaaaagaa gaactccgcc agcaaatcga
     1081 ggataagaac tgggatgcgc tgttgacaaa agtgccagtt aaagcaggag atttcttcta
     1141 tgtaccaagc ggtaccatgc acgctatcgg ggcgggtatc ttgattcttg aaacccagca
     1201 gtctagcgat accacttacc gtgtctatga ctttgaccgt aaggatgaca agggcaattt
     1261 acgtgaactt caccttgaaa aatctatcga tgtcttgaac attggtgaac ccgcaaatag
     1321 ccgtccagtg acaatcaaag cagatgatct acgttccact ctccttgtat ccaatgattt
     1381 cttcgcagtt tacaaatggg aaattactgg aaaagttaac tttgaaaaga cagctgacta
     1441 cagcttgttg agtgtcttgg ctggtcaagg tcagctaact gttgacggga aaaattatcc
     1501 aattcaaaaa ggcagccact ttatcctacc aagtgatgtt gaagcttgga ctctggaagg
     1561 gcaaggtttg gaattgattg ttagccatcc ataaaaagaa agggcctgag ttcactactc
     1621 aagtcctttt tgattacata aattgggcaa tgaagaccac aatgatgatg attggaatga
     1681 cgaaacgaag aaacgaagaa ggaagagcca gacttggaac agtccttgtt tccatgctct
     1741 ttcatcgaga tggagttcct ccattgcaag agccttttta aagatatagc ctgtaaaaag
     1801 tgaaaggtag agagctccaa atggcatgag gagattggaa accaagaagt ccatagcgtc
     1861 aaagaaggtc ttaccaaaaa tgtgaacatc cgccatgaca ccgtaagata gggctgaagg
     1921 aatgccaaag acaaaggtca aaattcctaa aataacactc catttggcac gtttgctgtt
     1981 atcctggttg gtgatattgc ccacattgat ctccagcatc acgacagaag aagtgacagt
     2041 cgcaaagagg gagcggacgt agaaaatggt tccaaaaggc atcttgtcaa agagttgagg
     2101 caagacgata aagagcaggc tgggtccccc ttcagactgg atattgaagg gggatcgagc
     2161 ttggaaaatg gctagacctg ccatgatgga tatcgagata ttcatggcta cgatggagat
     2221 tcctgactgg actagattgg ttttcttgtc caagtaagaa gcataggtca acatgactgt
     2281 aacccctagt gagagggcaa agaaagattg tcccagagca tagaggagac cagcgctagt
     2341 cagttttgaa aagtctggtt tgaggaagta aagaacccct tccatggcat ttggcaaact
     2401 gagagagcga ctgatgataa aaacaaagac gataaagagc aggggcatca tgactttcga
     2461 agctctttca atcccttttt gaaccccacg tgatacaatg aagatattca ataggataaa
     2521 ggccgcttga gctcctaggg caatggctgg atttgaaatg attgaagtaa ataactgagc
     2581 ataatcaccc gttccaccaa gttggaacaa tttcccaaac tcaataccta gatagactag
     2641 aatccatcct ccgataacac tgtaaaaaga taagaggata aagagggcaa aggcgccaat
     2701 ccaaccgata aagttgtact tgttattctt gcccagtttt ccaaaggttt tgatagcgga
     2761 aacgccagca ctacggccaa gggcaaactc agccagcagg agagggaaac cgattaaaat
     2821 agtggaaatg agaaagatta gtaaaaagcc tccaccgcca ttagcagcag tcatgtaggg
     2881 aaacttccaa acggaaccaa gcccgatggc cagccagcag atgctagaat aaaaccaagt
     2941 ttcgaccccc attgtgattt ttctgacata gttaaactct gatctccttt ttcaaaataa
     3001 gaaaaagcta cttgagttgt caaatagcct tctgtttgat tgatagactt gactatccta
     3061 tcatgttttc taaggtctgt caagaaaatc attttcaagt tttcacacct ttctcaaaaa
     3121 agttaaaaaa ttttctcaaa aacgcttgac tctgacctaa ggggaggggt tatactatca
     3181 ttgtaaggag gaaatcatgt accatataaa agaagctgcg cagctttcag gtgtctctgt
     3241 caagaccctg catcactatg acaagatagg actcttggtc cccttaaagt cggaaaacgg
     3301 ctatcgaacc tacagtcaag aggatttgga acgccttcag gtcattcttt actacaaata
     3361 tctaggcttt tctttagaga aaatagcaga gctgttaaag gaagaaagga cagatttatt
     3421 gccccatttg actaggcagt tggactatct aactcgcgaa aggcaacatc tggatacctt
     3481 gatttccacc ttgcaaaaaa ctattcaaga acaaaaagga gaaagaaaaa tgaccattga
     3541 ggaaaaattc acgggattta gctatcaaga caatcaaaaa taccaccaag aagcggtaga
     3601 gaaatatggt caagaagtca tgggacaagc gctcgaacgc caaaaaggtc acgaagacga
     3661 ggctacggcc gcctttaacc aagtctttca aactttggca caaaatcttc aagttggttt
     3721 acctgcaaca gcaaccgaaa accaggagca agcagccaag ctcttgcaag ccattcgcac
     3781 ttatggattt gactgctcta ttgaggtatt cggtcatatc ggtaaaggtt acgtctacaa
     3841 cccagagttt aaggaaaaca ttgacaagtt tggttctgaa acagcccagt acacgtcaga
     3901 tgccattgcg gcttacgttc agacaaatgc agaataaata ggctaggaat ttcctagcct
     3961 attttttact tcaaatcata aagccagtcg tcaccgtttt tgtagtaaaa gaattcactg
     4021 agatcttctt ctagaaacac acgaagcata tcagacatat catcggttgc aagttttaga
     4081 tgagaaagat tttcaaagtc ctcccaccaa actttccctt cgtctgaaga ctggagttca
     4141 ccagtaaagt gttctgtctt gtaaaaaagg acgacataac gataatcctt gtcgtcatac
     4201 cagtttttga taccacagag ttggggtttg gaaatgatca gaccagtttc ttctttcact
     4261 tcacgaatga cagcatcgac aaaggattcg ccacgttcaa catgaccacc aggaaaagta
     4321 atgccaggcc agtcgggact aactcggtct tggaccagga ccttatctcc gtttttaatc
     4381 atacacatgt taacaaattc gactgcctct cttctgttca ttcttcacaa cctttaatct
     4441 ttaatcataa tgcagacttc ccgccaccca gccggtacag agggcagaag tgatgttaaa
     4501 gccacccgtg tgggcattga tatccataac ttcgcctgca aagtggaggc caggtaccag
     4561 cttactttca agggttttag gattgatttc cttgagactg actccaccct tggtaacaaa
     4621 ggactttgca agggacattt ttccagttac aggaatttta agttctttaa tggactgaac
     4681 aagttgttct cgttcctttt cagtcagctg tttgactttt tcaggatatc cttgtacaaa
     4741 aaattcggcc aagcgttctg gtaacaaggt ttttaaagcg tttttcaagg atttttcccg
     4801 attttcttct agaaatgtaa ccaagtcctt ctcagaaagt tgaggcaaaa catcgagtga
     4861 gagaacctcc ccacctttga caaagctaga catgcgtagg gcagcaggac ctgacaaacc
     4921 aaagtgggta aagagtaaat catgagtgat gacatgctta ccataactta gggtcacatc
     4981 gtccagagaa atcccttgta aggctttatg tggaaaatct gttaataaag gactttcagc
     5041 agcctcaagc tcggtgatgg tatgcttaaa atggcgagca atctcgtgac caaaaccagt
     5101 cgaaccagtc gaaggataag acttaccacc tgttgtgaca atgagtttct cacaagtgaa
     5161 ggtttgatcc gctgacttaa ggacaaactg gtcatctact tttttaacag aaacgatttc
     5221 tatttgagta gcaacttgac cacctagttc ggtgattttc ttttccaaag cttcgataat
     5281 agtccgagac ttgtcactgg ctggaaagac gcgtccgtgg tcttcgacct taagtttaac
     5341 accattttct gtaaaaaagt tgatgatgtc atgattatcg aactgggaga aaacactgta
     5401 aagaaagcgt ccgtttccag gaattccagc tagcaggttg tctaagctac cattgttggt
     5461 cacattgcaa cgtcccccac cagtcccagc taattttttt ccaagtttcc gatttttttc
     5521 gatgaggagg gttttctgtc cataaaagct actggaaatc gtagccatca taccagcagg
     5581 tcccccaccg atgacaatag tatcaaaatg tttcatagct ctattgtacc acaaaaaaac
     5641 aagagatgat ggtcacctct tgtcaagaat gcaattaatc aatttcatag cccatcagca
     5701 aaccgccatc ttctgcatag aaactgcaga gaccagaggt tggtagaatt ttaatatccg
     5761 cttgtgggaa ggtttcacgg attcgctctg agagctgttg acaacatttt tcgttattgc
     5821 gttgggccat gacaatacgg ccaccagcat atccagcttt tactaactca tcataggcag
     5881 cttgaactga tttctttgat ccccttgctt tttgtagcaa ttcgagagtc ccagtttcac
     5941 tagcttttcc gaccatacga atgttgagaa ggccaacgac cgtaccgata agcttgctca
     6001 aacggccgtt cttcaccaag ttatcgactt tggctaggac aaagagcaac ttagtttttt
     6061 cttgataggc ggtgatagct tcaaccactt cttcaaaaga caagccctgg tcaatcaagt
     6121 cattcaattt ttctacgagt aggtcaactt caccaccagc agataaacta tcaatcacat
     6181 gaatcttagt gtcaggatgg tcttccagat aaatattctt tgctagttga gcactattgt
     6241 gactgccaga aagggtacct gtgatggtta ctaggaaaat gtttttggca ccttcaaatg
     6301 ctcgcaaata gtcatctggg cttggacaag ccgattttga agcttctgca gttgcataca
     6361 tggtttccat catttggtca atatcgagac tggcgtcatc aacaaagacc tgatcagcta
     6421 cttgaatggt taaggggaca cttacaaagg ttgtgttaat agctggtgtt ggcagttgac
     6481 gataatcaca accagagtca gcaataattt tccaagtcat agaaattctc catctttgtc
     6541 agttatacat tgacaaaggt tctgtctttt tttacaatta tatcatgaaa acccttgaaa
     6601 caaaagcatc atccgtctgt tgtgacaagt agaaagaaaa tgttatgtct gaacgtagaa
     6661 tctctgaaaa gtctcttgaa aatctcagaa aatcaaacca agaatccaat ttattaacca
     6721 gagaagccat tgaaacagcc ctcttgcaac tcttggaaaa aaaggagctg accaagatta
     6781 gtatttctga attggtcaaa cgtgcaggtg tttcgcgtgc ggccttttat cgcaattatg
     6841 actccaaaga ggaaatttta gagagcgtct ttaaacgaac tgtccacaat attatggaac
     6901 agctgcatca ttacgattta aagacagacc tttatttggt ttgggttcac cttttccggg
     6961 aggccagaaa ggaagctaga gtgattcaat tggccttgga ttaccatctg gaaaaaatct
     7021 ttgtccaagc catgcaggaa tttctagaaa aataccatgg gaaatcaaaa ggtgtcagct
     7081 cttatcttca ttccttctgg agctcagcca tcgtctctgt ccttctaaaa tggatcaagg
     7141 atggcatgaa ggtaccagct gaaaagattg cagatttagg tttaccattt tttaaaaaat
     7201 agagaaaaag gagaaaagag atgactgaaa aaagactggc ctgggatgag tattttgcag
     7261 cccaagccct actaattgcg aatcgttcca cttgtaaacg tgctaaagtg ggcgcgattc
     7321 tggtaaaaga taataaggtt atttccactg gttacaatgg ttcggtgtca gggactgagc
     7381 attgtattga tcacgaatgt ttggtcattg aaggccactg tgttcgcacc cttcacgctg
     7441 aggtcaatgc tatccttcaa ggtgctgagc gtggtgttcc caaaggcttt acagcctatg
     7501 taacccattt tccttgtctc aactgtacaa aacaattgct tcaggtcggc tgcaagcgcg
     7561 tggtttatat caaccagtac cgaatggacg actacgccca atacctttat caagaaaaag
     7621 ggacagaatt gacccattta ccacttgaga cagtacagac agctcttaaa gaggctgatc
     7681 taatgtaaaa attatcaaaa ataaatggct tagaaagatt tttaaaccgt tttttggtat
     7741 aataagaaga ataaattgaa agaaggaatt ccaaaaatgg gaaaaattga agttattaat
     7801 cacccactga ttcaacacaa attgtcaatc ttgcgtcgta cagatacttc tacaaaagct
     7861 tttcgtgagc tagtagatga gattgcaatg ttgatggggt atgaagtact tcgtgatctt
     7921 ccactagaag atgtggaaat cgaaacacca attacaaaaa cagttcaaaa acaattggca
     7981 ggtaagaaat tggccatcgt cccaatcttg cgtgcaggta tcgggatggt tgatggtctc
     8041 ttgagcttgg ttccagctgc taaagttggc cacatcggta tgtaccgtga tgaagaaaca
     8101 cttcaaccag ttgaatactt ggtgaaattg cctgaggaca ttgaccaacg tcaaattttt
     8161 atagtagacc caatgttggc aacaggtggc tcagcaatct tggctgttga ttctcttaaa
     8221 aaacgtggcg catcaaatat caaatttgtc tgccttgtat ctgctccaga gggtgtaaaa
     8281 gcccttcaag aagctcatcc agatgtagaa atctttacag cagccttgga tgaacgtttg
     8341 aacgaacacg gttatatcgt tccaggtctt ggagatgctg gagaccgctt gttcggtaca
     8401 aaataagatc gaaaagaaga atgacttgga aaagtatttc cagtcacgaa aggaggttgg
     8461 gtttttgttt ctgtctaatg aaagcagagc aaaaatttga ccttttttga ccaagatatt
     8521 ataatagtct tatcttcagt catttgacca acaaaattaa aactcaaaag gagaaatgaa
     8581 tgattcctgt agttattgaa caaacaagcc gtggagaacg ttcttacgat atttactcac
     8641 gtcttctcaa agaccgcatc attatgctga caggtccggt tgaagacaat atggctaact
     8701 ctgttattgc ccaattgctt ttcttggatg cccaagatag tacaaaagat atttaccttt
     8761 atgtcaatac accaggtggt tctgtttcag ctggtttggc aatcgtagat accatgaact
     8821 ttatcaaggc agatgtccaa accatcgtta tgggaatggc tgcatctatg ggaacagtca
     8881 tcgcatcaag tggagcaaaa ggcaaacgtt tcatgcttcc aaatgctgaa tacatgattc
     8941 accaaccaat gggcggtaca ggtggtggta cccaacaaac tgatatggct atcgctgcag
     9001 aacacttgct caaaactcgt aataccttgg aaaaaatctt ggctgaaaat tcaggtcagt
     9061 caatggaaaa agtccatgca gatgcagaac gtgataactg gatgagcgcc caggaaacac
     9121 ttgaatatgg ctttattgat gaaattatgg ccaacaattc attgaactaa tgatgataga
     9181 aggcaaactc gactgggttt gctttttttg gtataatagg gagagatttc ttagaaagag
     9241 gatttatcat gtttgaaaaa gtcaatcgct ctggcttgat tatctatctt tactataatc
     9301 gtgatgccaa aaaattgcag gattatggag atattaccta tcattccaag aaacatcgtt
     9361 acttacaact ctatgttcca actcaagaag tggagcaatt ggtcggacgc ttgagcaagg
     9421 aaaaatttat taaaaaagtt agggtttgtc atatccaaga gttggaaaca ccctttgtgg
     9481 gcaatcttta tcgataggaa aacgttatca tagaaaaagt tcaaggaaaa cattgacaat
     9541 tttctgataa ttcggtatat tcttaacaga ctatttaaga aataaggaga caaaaagatg
     9601 aagaaaaaat ttgccctatc gtttgtggcg cttgcaagtg tagcacttct tgcagcctgt
     9661 ggagaagtga agtctggagc agtcaacact gctggtaact cagtagagga aaagacaatt
     9721 aaaatcgggt ttaactttga agaatcaggt tctttagctg catacggaac agctgaacaa
     9781 aaaggtgccc aattggctgt tgatgaaatc aatgccgcag gtggtatcga tggaaaacaa
     9841 atcgaagtaa tcgataaaga taataagtct gaaacagctg aggctgcttc agttacaact
     9901 aaccttgtaa cccaatctaa agtatcagca gtcgtaggac ctgcgacatc tggtgcgact
     9961 gcagctgcgg tagcgaacgc tacaaaagca ggtgttccat tgatctcacc aagtgcgact
    10021 caagatggat tgactaaagg tcaagattac ctctttattg gaactttcca agatagcttc
    10081 caaggaaaaa ttatctcaaa ctatgtttct gaaaaattaa atgctaagaa agttgttctt
    10141 tacactgaca atgccagtga ctatgctaaa gggattgcca aatctttccg cgagtcatac
    10201 aagggtgaaa tcgttgcaga tgaaactttc gtagcaggtg acacagactt ccaagcagcc
    10261 cttacaaaaa tgaaagggag agactttgat gctatcgttg ttcctggtta ctataatgag
    10321 gctggtaaga ttgtaaacca agcgcgtggc atgggaattg acaaaccaat cgttggtggt
    10381 gatggattca acggtgagga gtttgtacaa caagcaactg ctgaaaaagc atcaaacatc
    10441 tactttatct caggcttctc aactactgta gaagtttcag ctaaagctaa agccttcctt
    10501 gacgcttacc gtgctaagta caatgaagag ccttcaacat ttgcagcctt ggcttatgat
    10561 tcagttcacc ttgtagcaaa cgcagcaaaa ggtgctaaaa attcaggtga aatcaagaat
    10621 aaccttgcta aaacaaaaga ttttgaaggt gtaactggtc aaacaagctt cgatgcagac
    10681 cacaacacag tcaaaactgc ttacatgatg accatgaaca atggtaaagt tgaagcagca
    10741 gaagttgtaa aaccataata gaaaaatgtt gaaataggga atgagccttt gactcactcc
    10801 ctgtttcgat atttaatact cttcgaaaat ctcttcaaac tgcgtcaacg tcgccttgga
    10861 ttatatatgt gactgacttc gtcagtctta tctacaacct caaagcagtg ctttgagcaa
    10921 cctgcggcta gtttcctagt ttgctctttg attttcattg agtataagaa cctatcaaaa
    10981 agtgagggaa aaccctcgga attataaata gaaagagtga atcttatgct ccaacaactc
    11041 gtaaatggtt tgattctagg tagtgtttac gcgctgttag ccctaggata taccatggtt
    11101 tacggaatta tcaagctcat caacttcgcc catggtgata tttatatgat gggagccttt
    11161 atcggttatt tcttgatcaa ttctttccaa atgaatttct ttgtagcgct tattgtagct
    11221 atgctagcga cagctattct tggtgtcgtg attgagtttc ttgcttaccg acctttgcgc
    11281 cactctactc gtattgctgt tttgattacg gctattgggg tttctttcct attggagtat
    11341 ggaatggtct atctggttgg tgccaatacc cgtgccttcc ctcaagcgat tcaaacagtt
    11401 cgatatgatt tgggagcaat tagcttaaca aatgtgcagt taatgatttt ggccatttcc
    11461 ttgattttga tgattttgtt acaagtcatt gtccaaaaga ctaagatggg gaaagccatg
    11521 cgtgcagtat cagtagatag cgacgcggca caattgatgg ggatcaatgt aaaccgtacg
    11581 attagcttta ccttcgcttt gggttctgct cttgcgggtg cggctggtgt tctgattgct
    11641 ctttattata actctcttga gcctttgatg ggggttactc caggtcttaa atctttcgtt
    11701 gccgcagtac ttggtggtat cggaattatt cccggtgcgg ctcttggtgg ctttgtgatt
    11761 ggtctattgg aaacctttgc gacagccttt gagatgtcag atttccgtga tgccattgtt
    11821 tatggaatct tgttgttgat cttgattgtc cgcccagctg gtatccttgg taagaatgtg
    11881 aaagagaagg tgtaaacgat gaaggaaaat ttaaaagtta atattctatg gttactcctt
    11941 ttgttagctg gctatagctt gattagtgta ctggtttcag tcggagtact taatctattc
    12001 tatgtacaga ttttacaaca aattggaatt aatattattt tggctgttgg tctcaactta
    12061 atcgttggtt tttcaggaca attttcactt ggtcatgctg gtttcatggc gattggtgcc
    12121 tatgcagcag ctattattgg ttctaaatca ccaacctacg gtgccttctt tggagctatg
    12181 cttgtagggg ctttgctttc aggagcagtt gccttacttg tcggcattcc aaccttgcgc
    12241 ttgaaggggg actatcttgc ggtagcaact ctgggtgttt ctgaaattat ccgtatcttt
    12301 atcatcaatg gtggaagtct tacaaatggt gcggcaggta tcttagggat tcctaacttt
    12361 acaacttggc aaatggttta cttctttgtc gtgattacaa ccattgcaac cttgaacttc
    12421 ttgcgtagcc caattggccg ttcaaccctc tctgttcgtg aagatgaaat cgctgctgag
    12481 tcagttgggg ttaatacgac taaaattaaa atcatcgctt ttgtctttgg tgccattact
    12541 gcaagtattg ctgggtcact tcaggcagga tttatcgggt ctgttgtacc gaaagattac
    12601 accttcatca actcaatcaa cgttttgatt attgttgtat ttggtggact cggttccatt
    12661 acaggtgcga ttgtttcggc tattgttctg ggaattttga atatgcttct ccaagatgtt
    12721 gctagtgtgc gtatgattat ttacgctttg gccttggtat tggtaatgat tttcagacca
    12781 ggtggactcc ttggaacgtg ggaactgagc ctatcacgtt tctttaaaaa atctaagaag
    12841 gaggaacaaa actaatggca ttacttgaag taaaacagtt aaccaaacat tttggtggtc
    12901 taacagctgt tggagatgtg actcttgaat tgaacgaagg ggaactggtt ggattaatcg
    12961 gtccaaacgg agctgggaaa accacccttt tcaacctttt gaccggtgtt tatgaaccaa
    13021 gcgagggaac agtaacccta gatggtcacc ttttgaatgg gaaatcacct tataagattg
    13081 cctctttggg acttggacgt actttccaaa atatccgtct ctttaaagat ttaacagttt
    13141 tagataatgt tttgattgct tttggaaacc atcacaaaca gcatgttttt actagtttct
    13201 tacgcttacc agctttttac aagagtgaaa aagaattaaa ggataaagct ttggaattgt
    13261 tgaaaatctt tgatttagat ggtgatgcag agactcttgc taaaaatctt tcctacggac
    13321 aacaacgtcg tttggaaatt gttcgtgccc ttgctacgga acctaaaatt ctcttcttag
    13381 atgaaccagc agcaggtatg aacccacagg aaacagccga attgactgag ttaattcgtc
    13441 gtatcaaaga tgaatttaag attacaatca tgttgattga acacgatatg aatctggtca
    13501 tggaagtaac agaacgtatc tacgtacttg aatatggccg tttaattgct caaggaactc
    13561 cagacgaaat taagaccaat aaacgcgtta tcgaagctta tctaggaggt gaagcctaat
    13621 gtctatgtta aaagttgaaa atctttctgt gcattacggt atgatccaag cagtccgtga
    13681 tgtaagcttt gaagttaatg aaggagaagt tgtttccctt atcggtgcca acggtgcagg
    13741 taagacaact attcttcgca ccttgtcagg tttggttcga ccaagttcag gaaagattga
    13801 atttttaggt caagaaatcc aaaaaatgcc agctcagaaa atcgtggcag gtggtctttc
    13861 acaagttcca gaaggacgcc acgtctttcc tggcttgact gttatggaaa atcttgaaat
    13921 gggagctttc ttaaagaaaa atcgtgaaga aaatcaagct aacttgaaga aggttttctc
    13981 acgctttcct cgtcttgaag aacggaagaa ccaagatgca gctactcttt caggagggga
    14041 acaacaaatg cttgccatgg gacgcgctct tatgtcaaca ccaaaacttc ttcttttaga
    14101 tgaaccatca atgggacttg ccccaatctt catccaagag atttttgata tcattcaaga
    14161 tattcagaag caaggaacaa ccgtcctctt gattgaacaa aatgccaata aagcacttgc
    14221 aatctctgac cgaggatatg tattggaaac aggaaaaatc gtcctatcag gaacaggaaa
    14281 agaactcgct tcatcagaag aagtcagaaa agcatatcta ggtggctaaa acaatccagt
    14341 ggattgtttt agtcggcaga tgaagattac gaagtaatca tcaatatagt ccgggggccc
    14401 tttttagtcg gtggatgaag attgcgaagc aatcatcaaa tccagaggat tgttttagcc
    14461 ggtagattga gattgcaaac aaatctgcat ctacattgaa agctgaattt ctaataattg
    14521 aaaaaatcga atgaaacgta tcttaccttc attcagggag ctcgatttta gagctctttt
    14581 tgctagctta ttcatacttt tttgaatttc gaaaaagaaa tgtaagcgtt tgatagattt
    14641 acaaaaagat tgtataatgg ggataagaat agaaaaggag aagtctcatg gcagttaaag
    14701 attttatgac ccgcaaggta gtttatatta gtccagatac aacagtatct catgcagcag
    14761 atttgatgag agagcaagaa ttgcaccgct tgcctgttat cgaaaatgat caattggttg
    14821 gtttggtgac tgagggaacc attgcacaag caagtccatc taaagcaaca agtctttcta
    14881 tctatgagat gaattatctt ctgaataaga caaaagtaaa agatgtcatg attcgcgatg
    14941 ttgtcactgt ctcaggctat gctagtctag aagatgcaac ttatctgatg ttgaaaaata
    15001 agattggtat tctccctgtc gtagataacc atcaagtata cggagttatt actgaccgtg
    15061 acgttttcca agcctttctt gaaattgcag gttatggcga agaagggatt cgtgtacgct
    15121 ttgttacaga agatgaagtt ggtgttcttg gaaaaattgt ttctttgatt gtagaagaaa
    15181 atttgaatat ctcccataca gtcaatattc cgcgtaagga tggtaaggtc attatcgaag
    15241 tgcaaatcga tggatcaatt gatttaccag ccttgaaaga aaaatttgaa gcaaatggta
    15301 ttcaagtgga agaaatcgct cgcacttcag caaaagtctt gtaagaaggg aagcccaaag
    15361 gcttcttttt tcatgaaaag gggattagag aaaaagatgg aaagaaatga taaaatatgc
    15421 tataatgaaa taatgtaaaa aaggagtatt tatggacatt tcagtaattc gtcagaaaat
    15481 tgacgcaaat cgtgaaaaat tagcttcttt cagggggtct ctttgacctc gaagggctag
    15541 aggaagagat tgccatcttg gaaaacaaga tgacagaacc tgatttttgg aacgataata
    15601 ttgcggccca aaaaacgtcg caagaattaa atgaattaaa aaacacttac aataccttcc
    15661 ataagatgga agagttgcag gatgaagtcg aaattttatt ggattttttg gctgaagacg
    15721 agtcagtgca tgatgaactg gtagcgcagt tagccgaact tgataagata atgaccagct
    15781 acgagatgac tctactcttg tcagaacctt atgaccacaa caatgccatc ttggaaatcc
    15841 atccaggttc tggtggtact gaggcgcagg actggggtga tatgttgctt cgtatgtata
    15901 ctcgttatgg taatgctaaa ggctttaaag tggaagtgtt ggattaccaa gcaggtgatg
    15961 aggctggtat taagtcggta actttatcat ttgaagggcc taatgcctat ggtctcctca
    16021 agtcagaaat gggtgttcac cgcttagtgc gaatctcacc atttgactct gccaaacgtc
    16081 gccatacctc tttcacatct gtagaagtga tgccagaatt ggatgatact attgaagtgg
    16141 aaatccgtga agatgatatc aagatggata ccttccgttc aggtggtgcc ggtggacaaa
    16201 acgtcaataa ggtttcaaca ggtgtacgtt taacccacat tccaactgga attgttgtcc
    16261 aatcaacagt ggatcgtacc cagtatggaa atagagatcg tgccatgaag atgttgcagg
    16321 ctaagctcta tcaaatggag caagataaga aggctgcgga ggtagattct ctcaaaggtg
    16381 agaaaaagga gatcacttgg ggaagccaaa tccgttctta tgtcttcacg ccttatacta
    16441 tggtaaaaga tcaccgaact agctttgagg ttgctcaggt agataaggtt atggatgggg
    16501 acctagatgg ttttatcgat gcttatctca agtggcgaat tagctaagat agaaaggaac
    16561 tcacatgtca attattgaaa tgagagatgt cgttaaaaaa tacgacaacg gaacaactgc
    16621 tctacgcggt gtttcggtta gcgttcaacc gggggaattt gcttacatcg taggaccttc
    16681 aggagcaggg aagtcaactt ttattcgttc tctgtatcgt gaagtaaaaa tcgataaagg
    16741 aagcctatca gttgctggtt ttaatctggt taagatcaaa aagaaagatg tcccgcttct
    16801 acgtcgtagt gttggggttg tcttccagga ttataaactg ttaccaaaga aaactgtcta
    16861 tgaaaatatt gcttacgcta tggaagtaat cggggaaaat cgccgtaata tcaaaagacg
    16921 agtgatggaa gttttggact tggttggatt gaagcataag gttcgttctt ttccaaatga
    16981 actctcaggt ggggagcaac agcgaattgc gattgcgcgt gcaattgtaa ataatcccaa
    17041 agtattgata gctgatgagc caacaggaaa tctggatccg gataattcat gggaaattat
    17101 gaatctcttg gaacggatta acctacaagg aacaactatt ttgatggcga ctcataatag
    17161 ccagattgta aataccttgc gccaccgtgt cattgccatt gaaaatggcc gtgtcgttcg
    17221 tgacgaatca aaaggagagt atggatacga tgattagtag attttttcgc catttatttg
    17281 aagccttaaa aagtttgaaa cgaaatggtt ggatgacagt agctgctgtc agttcagtca
    17341 tgattacttt gaccttggtg gcaatatttg catctgttat tttcaataca gcgaaactag
    17401 ctacagatat tgaaaataat gtccgtgtag tagtttatat ccgaaaggat gtggaagata
    17461 atagtcagac aattgaaaaa gaaggtcaaa ctgttacaaa taatgactac cacaaggtat
    17521 atgattcttt gaagaacatg tctacggtta aaagtgttac cttttcaagt aaagaagaac
    17581 aatatgaaaa attaaccgag ataatgggag ataactggaa aatctttgaa ggagatgcca
    17641 atcctctcta tgatgcctat attgtagagg caaacactcc aaatgatgta aaaactatag
    17701 ccgaagatgc taaaaaaatt gaaggtgtct ctgaggttca agatggcggt gccaatacag
    17761 aaagactctt caagttagct tcatttatcc gtgtttgggg actagggatt gctgctttgt
    17821 taatttttat cgcagttttt ttgatttcaa ataccattcg tattaccatt atttcccgca
    17881 gtcgcgaaat tcaaatcatg cgcttggtcg gagctaaaaa cagttatatc cgtggaccgt
    17941 tcttgttaga aggagccttt atcggtttat tgggagctat cgcaccatct gttttggtct
    18001 ttattgttta tcaaattgtt tatcaatctg tcaacaaatc gttggtaggg caaaatctat
    18061 ccatgattag tccagattta tttagtccgt tgatgattgc cctactattt gtgattgggg
    18121 ttttcattgg ttcattggga tcaggaatat ccatgcgccg attcttgaag atttaggtaa
    18181 aatagctgct tttatgagga gattgtaaaa tctccttttt tgctacaaga gtttttgaaa
    18241 agagatgcgc agaagaaaag agcttccaaa gaagtccccc agagaagact tcttgatggc
    18301 taatttgaac ttgaaatttg actcactaga aattttttag aaaaaagtgt aattttttta
    18361 taaaagcctt tacaaaaaaa attaaagtgg tataattaaa ttatagaagg tgcaaacgtt
    18421 ttcgtaaaac gtttgcctaa ataaaaataa aggagatttg aatgatgaaa gatacattca
    18481 aaaatgtctt gtctttcgaa ttttggcaaa aattcggtaa ggctttgatg gtagttatcg
    18541 cggttatgcc ggctgctggt ttgatgattt caatcggtaa gtctatcgtg atgattaacc
    18601 caacctttac accacttgtc atcacaggtg gaattcttga gcaaatcggt tggggggtta
    18661 tcggtaacct tcacattttg tttgccctag ccattggagg aagctgggct aaagaacgtg
    18721 ctggtggtgc tttcgccgct ggtcttgcct tcatcttgat taaccgtatc actggtacaa
    18781 tttttggtgt atcaggcgat atgttgaaaa atccagatgc tatggtaact actttctttg
    18841 gtggttcaat caaagttgct gattacttta tcagtgttct tgaagctcca gccttgaaca
    18901 tgggggtatt cgtagggatt atctcaggtt ttgtaggggc aactgcttac aacaaatact
    18961 acaacttccg taaacttcct gatgcacttt cattcttcaa cgggaaacgt ttcgtaccat
    19021 ttgtagttat tcttcgttca gcaatcgctg caattctact tgctgctttc tggccagtag
    19081 ttcaaacagg tatcaataac ttcggtatct ggattgccaa ctcacaagaa actgctccaa
    19141 ttcttgcacc attcttgtat ggtactttgg aacgtttgct cttgccattt ggtcttcacc
    19201 acatgttgac tatcccaatg aactacacag ctcttggtgg tacttatgac attttaactg
    19261 gtgcagctaa aggtactcaa gtattcggtc aagacccact atggcttgca tgggtaacag
    19321 accttgtaaa ccttaaaggt actgatgcta gtcaatatca acacttgtta gatacagtac
    19381 atccagctcg tttcaaagtt ggacaaatga tcggttcatt cggtatcttg atgggtgtga
    19441 ttgttgctat ctaccgtaat gttgatgctg acaagaaaca taaatacaaa ggtatgatga
    19501 ttgcaacagc tcttgcaaca ttcttgacag gggttactga accaatcgaa tacatgttca
    19561 tgttcatcgc aacacctatg tatcttgttt actcacttgt tcaaggtgct gccttcgcta
    19621 tggctgacgt cgtaaaccta cgtatgcact cattcggttc aatcgagttc ttgactcgta
    19681 cacctattgc aatcagtgct ggtattggta tggatatcgt taacttcgtt tgggtaactg
    19741 ttctctttgc tgtaatcatg tactttatcg caaacttcat gattcaaaaa ttcaactacg
    19801 caactccagg gcgcaacgga aactacgaaa ctgctgaagg ttcagaagaa accagcagcg
    19861 aagtgaaagt tgcagcaggc tctcaagctg taaacattat caaccttctt ggtggacgtg
    19921 taaacatcgt tgatgttgat gcatgtatga ctcgtcttcg tgtaactgtt aaagatgcag
    19981 ataaagtagg aaatgcagag caatggaaag cagaaggagc tatgggtctt gtgatgaaag
    20041 gacaaggggt tcaagctatc tacggtccaa aagctgacat tttgaaatct gatatccaag
    20101 atatccttga ttcaggtgaa atcattcctg aaactcttcc aagccaaatg actgaagcac
    20161 aacaaaacac tgttcacttc aaagatctta ctgaggaagt ttactcagta gcagacggtc
    20221 aagttgttgg tttggaacaa gtaaaggatc cagtatttgc tcaaaaaatg atgggtgatg
    20281 gatttgcagt agaacctgca aatggaaaca ttgtatctcc agtttcaggt actgtgtcaa
    20341 gcatcttccc aacaaaacat gcttttggta ttgtgacgga agcaggtctt gaagtattgg
    20401 ttcacattgg tttggacaca gtaagtcttg aaggtaaacc atttacagtt catgttgctg
    20461 aaggacaaaa agttgcagca ggagatctcc ttgtcacagc tgacttggat gctatccgtg
    20521 cagcaggacg tgaaacttca acagtagttg tcttcacaaa tggtgatgca attaaatcag
    20581 ttaaattaga aaaaacaggt tctcttgcag ctaaaacagc agttgctaaa gtagaattgt
    20641 aatatacttg aggttggaag ctgtattcca acctcttatt ttgggagaaa agaatgaaat
    20701 ttttaacact caatactcac agttggatgg agaaagaagc agaggaaaaa ttccagattt
    20761 tgcttgaaga tattcttgaa aaggactatg atttgatttg tttccaagaa atcaatcagg
    20821 agatgacctc gtcagaggtg gaggttaatg acctttatca agctttgcca gcagctgagc
    20881 ctattcacca agaccattat gttagactct tggttgaaaa gttgttagag caagggaaaa
    20941 attactactg gacctgggct tataaccata tcggctataa ccgctaccac gaaggtgtgg
    21001 ctatcttgtc taaaacacct attgaagcca gagaaatttt ggtttcagat gtggatgatc
    21061 caacagacta tcatactcgc cgtgttgccc tagctgaaac tgtagtcgat ggcaaggagc
    21121 tagcagttgc cagtgttcat ctctcttggt gggataaagg tttccaagaa gaatgggcac
    21181 gatttgaggc tgtcttgaaa aaattgaaca agccactttt actagctgga gattttaaca
    21241 acccagcagg tcaggaaggt taccaagcta ttttagctag tccattaggc ttacaagacg
    21301 catttgaagt tgctcaagag aaaagtggta gctatactgt tccgcctgaa attgatggct
    21361 ggaaagggaa cactgaaccc cttcgaatcg attatgtctt tactaccaaa gagttagcgg
    21421 tggaaaattt acatgtcgta tttgatggta acaagagtcc acaagtgagt gatcactatg
    21481 gcttgaatgc tatgttaaac tggaaataat aactgaaaag aggttggaac tataaaattc
    21541 cagccttttc ttactagaga agctactgga aatagcctaa ataagtgaga ctactgtaat
    21601 ggaataaaat atggtataat tgataagata gatagaatcg aggatgttat gtcatttacg
    21661 aaatttcaat ttaaaaacta tattagagaa gccttggagg agttaaaatt tacaactcca
    21721 acagaggtgc aagacaagtt gattcctatt gttttggcag gtcgtgacct agtaggagaa
    21781 tcaaaaacag gttcaggtaa gactcatact ttcttgttac cgattttcca gcaattagat
    21841 gaagctagcg atagtgtaca agcagtgatt actgcaccga gtcgtgagtt ggctactcaa
    21901 atttaccaag tagcgcgtca gatttcagct cactcagatg tcgaagttcg tgtggttaat
    21961 tatgtgggtg gtacggataa ggctcgccag attgagaaat tggaaagcaa tcagcctcat
    22021 attgttattg gaacaccagg ccgtatctac gacttggtta aatctggtga tttagctatt
    22081 cataaagcca agacatttgt tgttgatgaa gcagatatga ccttggatat gggattcttg
    22141 gaaactgttg ataagattgc tggcagtctt ccaaaagact tgcaattcat ggtcttctca
    22201 gcgactatcc cacaaaaact gcaaccattc ttgaaaaaat acttatcaaa tcctgttatg
    22261 gagaaaatta agaccaaaac ggttatttct gacaccattg ataattggtt gatttcgacc
    22321 aagggacatg ataagaatgc tcaaatttac cagttgactc agttgatgca gccgtatttg
    22381 gcaatgattt ttgttaacac taaaacgcgt gctgatgaat tgcattcata tctgactgct
    22441 caaggcttga aggttgcaaa aatccatggc gatattgccc ctcgtgaacg caagcgaatc
    22501 atgaatcagg tgcaaaatct ggattttgag tatattgtcg caacagattt ggcagcacgt
    22561 gggattgaca ttgaaggtgt cagccatgtc atcaatgatg ccattccgca agacttatct
    22621 ttttttgttc accgtgttgg tcgtactgga cgaaatggcc taccaggtac agctattacc
    22681 ctttatcagc caagtgatga ctcggatatc cgtgagttgg agaaattggg aatcaagttt
    22741 agtcctaaga tggtcaaaga cggggaattt caagatacct atgaccgtga tcgtcgtgcc
    22801 aaccgtgaga aaaaacaaga taaacttgat atcgaaatga ttggtttggt taaaaagaaa
    22861 aagaaaaaag tcaaaccggg ttataagaag aaaattcaat gggcggttga tgaaaagcgc
    22921 cgtaaaacca agcgtgctga aaatcgcgct cgcggtcgtg cagagcgtaa agctaaacgc
    22981 caaacatttt aatagaaatt gttggagtac tgagctccaa cttttttatt tatgagaacg
    23041 aactatctaa atcgaaacac tacattaaag actgcaaatt gcgattaaaa atggtataat
    23101 gataaagtta tatagtcccg ataagatggt aggtatttat tacgaagagt tttcctatca
    23161 gtactttgta actctataac aatatttttt aaggggggac atttttatgt cagaacgtaa
    23221 attattcacg tctgaatctg tatctgaggg gcatccggat aagattgcag accaaatttc
    23281 agatgcgatt ttggatgcta ttttagcaaa ggatccagag gcgcacgttg ctgctgaaac
    23341 agctgtatat actggttctg tccacgtttt tggtgaaatt tctacaaatg cctatgtgga
    23401 tattaaccgt gtggttcgtg ataccattgc agagattggt tataccaata cagaatatgg
    23461 attttctgct gagacggtgg gagtacaccc atctttggtg gaacaatctc ctgacatcgc
    23521 tcaaggtgtt aacgaagcct tggaggttcg tggaaatgct gatcaagatc cactggactt
    23581 gattggagca ggtgaccaag ggctcatgtt tggatttgca gtagatgaaa cagaagagct
    23641 catgccattg ccaattgcac tcagtcataa attggttcgt cgtctggcag aacttcgtaa
    23701 gtctggagaa attagctatc tccgtccaga tgcaaaatca caagttacag ttgagtacga
    23761 tgaaaatgac cgtccggtac gtgtagatac agtcgttatt tctactcagc atgatccaga
    23821 ggccactaat gaacaaatcc atcaagatgt gattgacaag gtcatcaaag aagttattcc
    23881 atcttcttat cttgatgata agacaaaatt ctttatcaat ccgacaggtc gttttgtaat
    23941 cggtggtcct caaggggact caggtttgac tggtcgtaag attattgtag atacttatgg
    24001 tggctactct cgtcatggtg gtggtgcctt ctctggtaaa gatgcgacta aggtggatcg
    24061 ttcagcttct tatgcggctc gctatattgc caagaatatc gttgcagcag gccttgctaa
    24121 gaaggcagaa gtgcagttgg cctatgctat cggtgttgcg caacctgttt ctgttcgtat
    24181 cgatactttc ggtacaggaa cagtagctga aagtcaactt gaaaaagcgg ctcgtcaaat
    24241 ctttgacctt cgccctgcag ggattatcca aatgctggac cttaagcgtc caatttaccg
    24301 tcaaacatcg gcttacggtc acatgggacg tacagatatt gatcttccat gggaacgttt
    24361 ggataaggta gatgctttga aagaagcagt aaaataagat tttaagaggg gaacgtcctc
    24421 tcttttttat agtttttaac tatactggga tactattctg aaaatccatt ttgcgaaagt
    24481 agagatttac atgtatagta gattgaaact agaatagtac acctcaactt ctaaaacatt
    24541 gttagaaatc gatttgactg tcctgatcga tttctcctgt tcttgtttca ttttactata
    24601 tttctctaaa aatgataaag gttgagattt ctcctcgtaa tagataatca tggggatatt
    24661 tcaatccaaa gttttattcg ttatcacttg actattgcaa ggttttctag agcaacagag
    24721 tcatggaatg gactcatggt tgagatttct ccttgttgct tggacttcat tcaaaagtct
    24781 gttacccaag ccttgttcaa acttctaata cactagctgt ttccatagca tgacttctgt
    24841 actagacttt cttttccgaa taaatagata gaaccacaga atctagtaaa cctagaatta
    24901 aaattatggt ataatattag caataaaaga aatctggagg attagaatca tggtatcaac
    24961 gaaaacacaa attgctggtt ttgagtttga caattgcttg atgaatgcag caggtgtggc
    25021 ttgtatgacg atagaggagt tagaagaggt caaaaactca gcggcaggaa cctttgttac
    25081 taagacagcg accttggact tccgtcaggg gaatcctgag ccacgctacc aagatgttcc
    25141 acttggttcc atcaactcta tgggcttgcc aaataatggc ttagactatt atttggatta
    25201 tcttttagat ttgcaggaaa aagagtcgaa ccgaactttc ttcttatctc tggtcggcat
    25261 gtctccagag gaaacccata ctattttgaa aaaagtccaa gagagtgatt ttcgtggtct
    25321 gactgagcta aatctttcct gtccaaatgt tccaggtaaa cctcagattg cctatgattt
    25381 tgagacaaca gaccggattt tggcagaagt gtttgcttac ttcaccaaac ctcttggaat
    25441 taaattgcca ccttattttg atattgttca ctttgatcaa gcggcagcta ttttcaacaa
    25501 atatccgctc aagtttgtca actgcgttaa ctctatcgga aacggcctct atatagaaga
    25561 cgaatctgtc gttattcggc ctaagaatgg ttttggtgga attggtggag aatacatcaa
    25621 accgactgct ctagccaatg ttcacgcctt ttatcaacgt ttaaatcctc aaatccaaat
    25681 tatcggaaca ggtggcgttc tgactggtcg agatgccttt gaacacatcc tctgtggagc
    25741 aagtatggtg caggtgggaa cgacccttca caaagaaggc gtcagtgctt ttgaccgcat
    25801 taccaatgaa ctgaaagcaa tcatggtgga aaaaggctac gagagcttag aagatttccg
    25861 tgggaaattg cgctatattg actaaattaa atcgaaaaat ctgaagaaag gagagacgat
    25921 gctagccatt gaagaaagtc agaagttgac tttatcaaat ttaccgagcc tgagcctatt
    25981 tacagggaca gatcagggtc agtttgaagt gatgaagagt caagtgttga aacagattgg
    26041 gtatgattct gctgacctca actttgccta ctttgatatg aaagaagtag tttacaagga
    26101 tgtggaactg gagttggtca gccttccttt ctttgcggat gagaaaatcg tgatattaga
    26161 ttattttatg gatatcacga ctgctaagaa acgctttttg acagatgatg agcttaagtc
    26221 atttgaggaa taccttgaca atccttctcc aacaaccaag ttgataatct ttgcagaagg
    26281 aaagctggat agcaaaagac ggttagtcaa attacttaag cgtgatgcca aggtcttcga
    26341 tgcagtagaa gtaaaagaac aagaattgcg ccagtacttc caaaagtgga gtcagaaaca
    26401 aggtctgcag tttaccaatc attcttttga aaatctcctc atcaagtcgg ggtttcaatt
    26461 tagcgaaatc cagaaaaatc ttctcttttt acagtcctat aaggtggatt ctgttatcga
    26521 ggaagaggat attgttaacg caattcccaa gaccttgcag gacaatattt ttgatttaac
    26581 tcagtttatt ctgactaaaa agatggatca ggcgcgcgat ttggtgagag acttgacctt
    26641 gcaaggggaa gatgaaatca aactgattgc ggtcatgttg ggacaatttc ggacttttac
    26701 tcaggtgaag attttggcgg agtctggcca aacagaatcg cagattgcaa gtagtttagg
    26761 tagttatctg ggacgtaacc caaatcctta tcaaatcaag tttgcattaa gagattcgag
    26821 aggactttct ttgagctttc tgaagcaagc tatttcctat ttgattgaga cagactatca
    26881 gattaagaca ggtctttatg aaaaaggttt cctttttgaa aaggcactct tacagattgc
    26941 tagtcaggtc aattgacatt tgttgaaact acaacccgcg tcaggattat cttgtcgtgg
    27001 gtttcttgct gatttgctta ttttattaga aagtatgtca gtgctttcct tatctataag
    27061 caaaaaactt gaaaaaagtg aatgtttgcg ttatcatttt catatagaaa gaaaaagagg
    27121 tattacagat ggctattatc ttaccagaac ttccatatgc atacgacgct ttggaaccat
    27181 acatcgatgc ggaaacaatg catttgcacc atgacaaaca ccatcaaact tatgtcaaca
    27241 atgccaatgc agctttagaa aaacaccctg aaatcggtga agaccttgaa gccttgcttg
    27301 ctgatgtaga atctatccca gctgatatcc gtcaagcact tatcaacaat ggtggaggac
    27361 acttgaacca cgctcttttc tgggaattga tgactcccga gaaaacagct ccttcagcag
    27421 aactggcagc agcaatcgat gcaacatttg gttcatttga agaattccaa gcagccttca
    27481 ctgcagcagc aacaactcgt tttggttcag gttgggcatg gttggttgtc aacaaagaag
    27541 ggaaacttga agtgacttca acagcaaacc aagacacacc aatctcagaa ggtaaaaaac
    27601 caatcttggg cttggacgtt tgggaacatg cttactacgt gaaataccgc aacgtgcgtc
    27661 ctgactacat caaagctttc ttttcagtaa tcaactggaa taaagtagat gaattgtacg
    27721 cagctgctaa ataatgatag ttggagggaa gaattgttct tctctttttt aggttataag
    27781 attctggtct gacaaaatcg tcagactttt ttcattttta tgagaaacgt gctaactgct
    27841 agaaattatg gtagaataaa gtattaagta atcgtggaaa aaggatatct atgcgaaaag
    27901 aaattgcacc tgaattatac aactataaca agtttcctgg tccggagttc catttacacg
    27961 gggacaaggt cgaaacggaa gggatagctt tttccttggt tgaaaatatc aaggatgcct
    28021 ttgatgtgac gacttttaat cagcgttttt cagaagtctt aaccaagttt gattatatcg
    28081 tgggggactg gagcaacgaa cagcttcgcc tacgaggttt ttacaaggat gaccgaacag
    28141 aagaaaaact tgaaaaaatc agtcgtttac aagactacct tttagagtat tgtagttatg
    28201 gttgtgccta ttttgtctta gaaaatgaag cccctaagcg agcatcattt gacaagaaaa
    28261 tgcgtaagac ggaagaagaa acaccttcta gaaaaggaaa gaaaccgact caaactaaac
    28321 gaaagtcgaa tgcagataag aaaaatagac gtcgtcagaa agaccagcat tctcagaaag
    28381 aggacaaggg acaacgtcat tttgtcattc gtcagaagtg agtagagaat aaggagaaga
    28441 aatgaaaccc tcaattcata gtttgccaca tcaaaccatg caggaatggg tattggagca
    28501 gggagaaaag aaattccgtg cagatcaaat ctgggaatgg ctctaccgta aacgtgtgca
    28561 gtcatttgaa gaaatgacca acctttccaa ggatttgatt gctaagctca atgaccagtt
    28621 tgtggtcaat cccttgaaac aacgtattgt gcaagagtct gctgatggta cagtcaaata
    28681 tctctttgaa ttgcccgacg gtatgttaat tgagactgta ctcatgcgtc aacactatgg
    28741 tttatctgtc tgtgtgacca ctcaggtcgg ctgtaatatc ggttgtacct tctgtgcctc
    28801 tggtttgatt aagaagcagc gtgacctcaa taacggtgaa atcgtagcgc aaatcatgct
    28861 ggttcagaaa tactttgatg agcgtggtca ggatgaacgc atcagccata tcgttgtcat
    28921 gggaatcggt gagccctttg ataattacaa caatgtcttg aatttcgttt gtactatcaa
    28981 tgatgataaa ggaatggcta tcggtgctcg tcacatcacg atttcaacct caggtttggc
    29041 tcataaaatt cgtgactttg ctgatgaagg ggttcaggtc aatcttgccg tgtctcttca
    29101 cgcacccaac aatgaacttc gttcaagtat catgaaaatt aaccgtgcct ttccgattga
    29161 aaaactcttt gctgctattg aatactatat tgagacaaca aaccgtcgtg taacctttga
    29221 atatatcatg cttaatgaag tcaacgacgg tgtagaacaa gccttggaat tggctgaatt
    29281 gctcaagaac atcaagaaat tgtcttatgt aaacttgatt ccttataacc cagttagtga
    29341 gcatgaccaa tatagccgta gtcccaaaga gcgcgtgttg gctttctatg atacgcttaa
    29401 gaaaaaaggg gtcaactgtg tggttcgtca agagcatggt acagatattg acgcagcttg
    29461 tggacaattg cgctctaaca caatgaaacg tgaccgccag aaggcagtcg cagcggtaaa
    29521 tccttaatga tgaagaagac ttataatcat attttggtct ggggagtcat tttctatagc
    29581 atttgcattg tctgtttttg ctttactcct caagaacaat ctaccgtggg agtgggaact
    29641 ccaggtattc agcatcttgg acgcctggtt tttcttttga ctcctttcaa ttctctctgg
    29701 aaactgggcg aagtgagtga cattggacaa ttatgttgga tttttttaca aaatatcctc
    29761 aatgtcttct tgttttttcc tctgattttc caactccttt atctatttcc aaatttgcgg
    29821 aaaacaaaaa aggtccttct ttttagtttt cttgtgagtc ttggaatcga gtgtacgcaa
    29881 ttaatcttgg actttttctt tgatttcaat cgcgtctttg agattgatga tttgtggacc
    29941 aacactttgg gtggctatct ggcttggctc ctttataaac gattacataa aaacaaggta
    30001 aggaattaaa atgagtattt tagaagttaa aaatctgagt cacggttttg gtgaccgtgc
    30061 aatttttgaa gatgtgtcct tccgtctcct caagggagaa catatcggcc tggtcggtgc
    30121 caatggtgaa ggaaaatcaa cctttatgag tatcgtgact ggtaaaatgc tgccagatga
    30181 aggaaaggtt gagtggtcca aatatgtgac ggctggttac ttggatcagc actctgtcct
    30241 tgctgaaaga cagtcggtgc gtgatgttct ccgtacggct tttgatgagc ttttcaaagc
    30301 tgaagctcgt atcaatgacc tctatatgaa aatggctgaa gacggcgcgg atgttgatgc
    30361 tctcatggaa gaagtaggag aacttcaaga ccgtctggag agtcgtgatt tctatacctt
    30421 ggatgctaag attgacgaag tagcgcgtgc tcttggtgtt atggactttg ggatggatac
    30481 ggatgtaact tctttgtcag gtgggcaaag aaccaaggtg cttttggcaa aacttctcct
    30541 tgaaaagcct gatattttgc tgttggacga gccgaccaac tacttggatg ctgagcatat
    30601 tgattggctc aagcgctatc tccaaaacta tgagaatgcc tttgttctta tttcgcacga
    30661 tattccattc ctcaatgacg ttattaatat tgtctatcat gtggaaaatc aacagctgac
    30721 gcgttactct ggtgactact accagttcca agaagtttat gctatgaaga aatctcagct
    30781 agaggcagcc tacgaacgcc agcagaaaga gattgcagac ctcaaggact ttgtggctcg
    30841 taataaagcc cgtgttgcaa ctcgtaatat ggctatgtct cgtcaaaaga aattggataa
    30901 gatggatatt atcgaactcc aaagtgagaa accaaaacca tcctttgatt tcaaaccagc
    30961 tcgtacacca gggcgcttta tcttccaagc caagaacttg caaattggtt acgaccgtcc
    31021 tcttactaag cctttaaatc ttaccttcga acgcaatcaa aaggttgcga ttattggtgc
    31081 taatggtatt ggaaaaacaa ctctcttgaa gagtctctta ggcattatct cgccaatcgc
    31141 tggggaagtg gagcgtggag attatttaga acttggttat tttgagcagg aagtagaagg
    31201 cggtaatcgc caaactcctc ttgaagctgt ctggaatgcc tttcctgccc ttaatcaagc
    31261 agaagtccgt gcagcccttg cccgttgtgg tttgacaacc aaacatattg aaagccagat
    31321 tcaagtatta tcagggggag agcaagccaa ggttcgtttc agtctcttga tgaatcgtga
    31381 aaacaacgtt ttagtgctgg acgagccgac caaccatttg gatgtggatg caaaggatga
    31441 gctcaaacgc gctctcaaag aatacagggg atctatcctt atggtctgcc acgagccaga
    31501 cttttatgaa ggttggatag accaaatatg ggattttaat aatttaactt aagaatgaga
    31561 gtaaaagaaa tacagtaccg aatgtggata ctgtattttt ttggttttta agatttaaaa
    31621 tcgtagtctt tcaccacttc gatgctgtcg attgtgatag cagtagttgg cttgtctttt
    31681 tcatcttttt cggctttagc aatcttatcc acaacatcca taccgtcaat cacttgacca
    31741 aagactgggt gtttgccatc tagactaggg tttccacctt ctttgtaggc ttcgataatt
    31801 ttctgtggat acttgcttgt agggagttta gaagaggtat ctgtagagtt ttggttgatg
    31861 aagaactggc tgccattggt gtttggttga ccagtattag ccatagcaag agcaccacgg
    31921 atgttataca aataaggagt aatctcgttc ttgaaaccag ttcctttgtc tttagtctta
    31981 tccttgtcat gccatatgga ctgaccacct gtaccgtccc cttttggatc tccagtttgg
    32041 accataaagc catcgatgac acggtggaag gtaataccgt tatagtagcc ttctttggcg
    32101 tgagtgagga aattttcaac cgctagagga gcgagtttag ggaagagttt aatgcgaata
    32161 tcaccttggc ttgtgtggag aatcacttcg gcttcatctt cagcaacttc cttagatagt
    32221 tgtgggaaat tggcgttttc gtttgttaaa gcatcgttta actccttggc agattgggca
    32281 gctactttgg aactttcttc agcagcaaga ctggaatcaa cataatcatc accacgcaga
    32341 ctgcgttgga cgctgctaca cccagctagg gctacagtag acagtaaaag aagggttgct
    32401 agttttttca ttctgatcct ctcaaaaatt catttatacc attgtaccct aaaaggaagt
    32461 ggatttcaaa tattagtagt ttacagaact ggcttagata atagttggga tttcatcatc
    32521 atcttgaaaa tcttcaactt cttcaacttc ttcaacttct tccccagtaa atggtttctg
    32581 aaaagaactt agtataaaac gggcaattgg ttcaacaatt aggaaattta gaggaagagc
    32641 tgcaataaag tttacgaacc aggcgcttag ataggtattg ggtgtgatag caatgccatt
    32701 gtaaaaaagt ccatagaagg acatgaaagt gaccagctaa ttcttcttct gaaaaatgct
    32761 catgaagggc ttgatccagg tcgcaagatt tcattttaga aagtccagta actcccttct
    32821 tcttcaggat attctttttc atggtattat ttagatggtc aagtgagtca aaagaagttt
    32881 cgacaagagc ataccctttt tctaccagag tttttagatg tttaggggca tcaataccat
    32941 aagtgtactc aaagtatttt ggaaaccaga tcttaagaaa gctcgtaaag ctagccagtt
    33001 ctctaaacgc taagaactag ctacaacaca gcatttacaa cacttcatgg ttcacaccat
    33061 ggggtgtttt ttaatgattt taagcaaaat tcacacttaa ttgaaagtta tttaaattag
    33121 atgaaaattt attttttgaa aaagtgggaa aacattgatt ctaagcgaat tttgaatcat
    33181 attcgaatgg gagtttttaa aatcatgttt cagtggtaaa agtaccaaac tggattctcc
    33241 atagtaaaat gatatctcct gctagtgaat catctgctaa taagtccata tttcgcttgg
    33301 gacttagaag ccaagtatcc aactctcgtt caggcgatat gaagggtttg acttggtgtt
    33361 ctttataaaa tttttctaaa atagtattca cggatagcat ctttcaaaca tttatttcct
    33421 attctaacaa atttctaaat gctttaagaa atttatagtc tgtcaagttt ttttcttgac
    33481 acctgccaga aatctgctat aatagaacat gtgctaaata gctcagctat ttcaccgaaa
    33541 taaaaaatta aaggaatgag actttaaaaa tggcagttaa aatccgtttg actcgtatgg
    33601 gttctaagaa aaaacctttc taccgtatca acgtagcaga ttcacgttca ccacgtgacg
    33661 gacgtttcat cgaaacagtt ggaacttaca acccacttgt tgctgaaaac caagtaactt
    33721 tgaaagaaga ccgcgttctt gcatggttgg ctaatggagc tcaaccttca gacacagtac
    33781 gtaacatcct ttcaaaagaa ggcgtattga aaaaattcca cgattctaaa ttctcaaaat
    33841 aagtttaaag taggttgaca gatggatacg attgaaaatc tcattattgc gattgtgaaa
    33901 cccttaattt cacaaccaga tgccttaact atcaagattg aggatacacc agaatttttg
    33961 gaatatcatt tgaatcttga tcaaagcgat gtgggtcgtg taatcggtcg taagggtcgc
    34021 actatttctg cgataagaac gattgtctac tctgtcccaa ctgaatacaa aaaagtaaga
    34081 atcgttattg acgaaaaata agaagggcgg gacggatgtc tcgctttttt gtgtggaata
    34141 gaagatatag taaaatgaaa taataacagg acaaatcgat caggacagtc aaatcgattt
    34201 ctaacaatgt tttagaagta aaggtgtact attctagttt caatatacta taaaagattg
    34261 aacgtttagg caattccaag actacttaat cgaacattaa cagcagtctc ggactgatga
    34321 aggtcttttt atgaagttag tggaggaagt cggagaagta gccgaggttt tgaataggcg
    34381 ctctggtcga aaagaaggga taggattcga acgaggaact tgccagagaa ctggctgata
    34441 tcatttacta caccgtcgca atcgtagcta ttaatcatat tgaccttacc aagactgtct
    34501 ttgagaaaga caaaaccgca gccgttaatt accaacataa acataatttg gaaggattat
    34561 tgaagggaaa gtagagatat tgtccccttc ctccaacttg agaaaatcct tggaacgtga
    34621 taaaataaag gataaggatt tttaataatt cattatatag aatcagtgga tttctttatg
    34681 aagaggtttg aagtatctac agaaattggt agcctctctg ttacttatca aaagcaaaag
    34741 aaagtgctag tttgtctaaa cggagcaggt ttgctaccaa gttatgaaaa tttttcactt
    34801 atacttgaaa aacctcctcc tacaattggt tatttgacaa ttgattttcc gaacacaggt
    34861 aggagtccga ttcatgacca agctggaaaa aatctagata atcttgcaga tgcggtttat
    34921 gaagtacttg aagaattagg aatttctgaa tatatacttt gtgcacatag ttggagtgga
    34981 attttagctt gcaaattact cgagaaacca attaagcgtc agactttagt agcaattgaa
    35041 ccgacaacta aaaaagtcat gtttgctgat ttttcagaaa atccttatcc agaaatggaa
    35101 gagcagatga ggctgattga cgagtgtggt cctgaacttt attttaagaa cttaactcaa
    35161 gcaacattta gtcctgaaac gaataaaaaa atctgggaat taatgcaaga aaaaggctta
    35221 gagttggaaa atcaagatcc agaatttcag atatctggag agattactga ggaagatttt
    35281 gaggatgtat ctatagaagc tcatattcct gtatttgttt tttgtcagcc ttatagagaa
    35341 aaagagtaca gagaatcaga atattggact tccaatacta aactcatttt aggagggaat
    35401 caccattatt tacaatggtc agaatcggaa aaaattgcgg ctattattcg agaattgtta
    35461 gaataagatg gaaaaaagga gattacagga gacaagatga actactttaa tgttgggaaa
    35521 atcgttaata cgcagggatt acagggtgag atgcgagtct tgtctgtgac ggattttgca
    35581 gaagaacggt ttaaaaaagg agctgagctg gctttgtttg atgaaaaaga tcagtttgtc
    35641 caaacagtga ccatcgctag ccaccgtaaa cagaagaact ttgacattat taaattcaaa
    35701 gatatgtacc atatcaatac tatcgaaaag tacaagggat acagtctcaa ggtcgctgag
    35761 gaagatttga atgacctaga cgatggtgaa ttttactatc acgagattat cggtttggaa
    35821 gtctatgagg gtgatagctt ggttggaacc atcaaggaaa tcctgcaacc aggtgctaat
    35881 gatgtctggg tggtcaaacg aaaaggcaaa cgtgatttgc ttttacctta tatcccacca
    35941 gtggttctca atgttgatat tccaaataaa cgggtcgatg tggaaatctt agaagggtta
    36001 gacgatgaag attgatattt taaccctctt tccagagatg ttttctccac tggagcactc
    36061 aatcgttgga aaggctcgag aaaaagggct cttggatatc cagtatcata attttcgaga
    36121 aaatgctgaa aaggcccgtc atgtagatga tgagccctac ggaggcggtc agggcatgtt
    36181 gctcagagta caacctattt tcgattcctt tgatgctatt gaaaagaaaa atccgcgcgt
    36241 tattctcctc gatcctgctg gaaagcagtt tgatcaggct tatgctgaag atttggctca
    36301 agaggaagag ctaatcttta tctgtgggca ctatgagggt tatgatgagc gcattaagac
    36361 cttggtaaca gatgagattt ccctaggcga ctatgtcctc actggtggag aattggcagc
    36421 tatgaccatg attgatgcta cagttcgcct gattccagaa gtgattggca aggagtctag
    36481 ccaccaagat gatagttttt cttcaggtct tttagaatat cctcagtaca cacgtcctta
    36541 tgattatcga ggcatggtcg tgccagatgt attgatgagt ggccaccatg aaaagattcg
    36601 tcagtggcga ttgtacgaga gtttaaagaa aacctacgag cgcagaccgg atttacttga
    36661 acattatcaa ctgacagtag aagaagaaaa aatgctggca gaaatcaaag aaaacaaaga
    36721 ataaaggaga aacctatgca agtaatcaaa cgtaatggcg aaattgctga atttaatcca
    36781 gataagattt accaagccat cttgaaggca gcccaaactg tttatgtttt gacagatgat
    36841 ttgcgtcaaa atcttgctca agtcactaag aaggtagttt tggatttaca agaagccaag
    36901 gtggaacgtg cgactatcag tatgattcaa tctatggttg aacatcgttt attgggcgca
    36961 ggttacatta ccattgcaga acactacatt tcctatcgtt tacaacgtga cttggaaaga
    37021 agtggttatg gagatcatat cgcagttcat ttacattttg aacaaattcg ctaagaaaaa
    37081 agagtgggat gaagcaacta catctcactc tttcttaaat ctattttttt gggctgtttg
    37141 gacagttgag gggacaaatc gcaggcgttg aatatcgctt atacggataa tccggctcac
    37201 atttttggaa aaatttttca cgataatttg ctggccttgc tgatcatatt tgatgatgtc
    37261 acctgtaaag cttgtctcag ataagataag gtgaacagcg gtttgtttct ggatagcctc
    37321 ttcaatagtg gctgtaaggg agttggtttc agtctggtca ggttggtcat atccatttaa
    37381 aaaatcatgt attttattta gaacttgctg gaatttatgt ctcatattgg cctcccttct
    37441 tttatatcaa tattatataa aattttccta aaatctacaa gattttacga ataaactaat
    37501 gaaactaaaa aaggagaaga aaatggcaga atttacattt gaaatcgaag agcacttgtt
    37561 gactctttct gaaaacgaaa aaggttggac caaggaaatt aaccgtgtga gctttaatgg
    37621 tgcccctgca aagtttgata ttcgtgcttg gagtccagac catactaaaa tgggcaaagg
    37681 gattactctc tcaaatgaag aatttcaaac gatggtggat gcctttaaag gcaactaata
    37741 ctcttcgaaa atctcttcaa accgcgtcag ctttgccttg ccgtatgtat ggttactgac
    37801 ttcgtcagtt tcatccacaa cctcaaaaca atgttttgag ctgacttcgt cagtttcatc
    37861 cacaacctca aaacagtgtt ttgagcagcc tgcggctagc ttcctagttt gctctttgat
    37921 tttcattgag tataagtttt caaaataaaa gaaaaggata ttgagtcatg ataactcggt
    37981 atcctttttt agttagtaaa gtaagcctga tttttaaggc gttgaagtag gggacggcta
    38041 ataaaactaa tagccagaag tttgccaagg tctggaatga taaagggaag aacccccaca
    38101 acaagagctt tttcaaatgc cattccagct aggaaatgca agctgagaat cccgccgaca
    38161 aagacaaggg catcacccaa gaggtttgca agaaaaatct taacaacacc actcttgctg
    38221 ttggttagag aggaagtaag tccagagtaa acgagataaa accaaagata gcctgcagta
    38281 gggccaacta aagcctgaaa accagctcca cctcctgcaa agacaggaag accgatagca
    38341 cctagaagaa gatagagtcc agcagaaagt acagcctctc tcggtctaaa gacagtagca
    38401 atcaagccga ttgcaaagtt ttgcagagtg aagggaacag gtccaattgg aagactgatt
    38461 tgtgccaaag cagcaatgag agcagcccca atagcaggga tagcataaac gtgagctttt
    38521 ttcaaaatag ttaacctctt ttctaatgta tagtaactat tatactcata taggagtcga
    38581 tgtcaaccat tttttaaaaa caaggtaaca aaaactgtaa cagccgattt tcgtgactag
    38641 tcaagttgga taattttttt gttgattcga aaatcattaa aaaagaagca gacatattta
    38701 tatcagcttc ttttgtttcg attaacgcat ggttacaaat tcttcagatg aagttgggtg
    38761 aatcgctaca gttgcgtcaa agtcagcctt ggttgctccc attttgatag caacagcaaa
    38821 tccttgaatc atttcatcaa cgccgtagcc aattccgtga agtccgacaa ctttttcttc
    38881 tgaagcagct gttatgagtt tgaaacggga ttcttgacgg ttgcgagtgc aagcagagta
    38941 catagatgca aagcttgatt tataaacctt gatttggtct tgaccgtatt ctttaatagc
    39001 ttgctcttct gtcaaaccaa cagttccgat agcagggtgt gaaaagacaa cagttggaat
    39061 agttgagtaa tccatttttg cagtagtttt tccgttaaag agacgttcag atagggtacg
    39121 tccggccttg attgcaactg gagtcagttc tttctcgccc gttacatcac ctagagcata
    39181 gattccctca acgacggtat tttggtattc atctacttgg ataaagccac gttcattcag
    39241 agtcactcca gctttttcaa gttgcaagcc cttaacgttt ggacggcggc ctgtagccca
    39301 gataacttgg ctagctgtgt gactagtacc atcttcgaaa tgaatggtaa tgccgtcagt
    39361 agttttttct aacttgacag ggactttgtg agtgtgaagt ggtaagtttg ttctttccat
    39421 ttccttgacc aaaccttcaa cgatgtagga atcaaaacca cgtaaaggac gatcgcggcg
    39481 aacaaagaga tctgtcttga caccaaaagt gtggagtacg ccagctaatt caacggcgat
    39541 ataaccagcg cctagaatgg caactgactc tggaagttct tcccaggcaa atacatcatc
    39601 agaagagcca cctagctcag caccaggaat attaggaata cttggatggg cacctgtagc
    39661 aatcacgata tgtttagcac gaatcagttc accatttacg cttacagtat gagaatctac
    39721 aaattcagca tgaccttcaa tcaagtctac accgttgcgt ttaaaactgc catcataaga
    39781 agaacgagcg cgatcaatgt aggattcacg attgcgacgt agggttgcaa agtcaaagtt
    39841 aagatcagta gtcttaaagc cgtagtcttc tccaaattga tggaaagtct cagcgatttg
    39901 tgccccgtac cacatgattt ttttaggaac acaaccgacg ttgacacagg ttccacctaa
    39961 tttcttttcc tcaataacgg ctgctttggc tccatgttca ccagcacggt tcatggtagc
    40021 gattcctccg ctacctccac cgatagcaat gatatcatat tctctcattg aaaactcctt
    40081 tgtaatcttt taaatagtag agcgtctttt ttgatagtta tattttattc tttttttgat
    40141 ttgattgcaa aaatctgcta cgcacaaaaa attgcaaaaa agcttgccaa ataagtcaag
    40201 atagtgtatt atatatctag tacaatagaa aagcaaaatt ccgaacgatt gtttaatcct
    40261 gaactgtatt gtttgctgga gggaaatttg gtataatagc aaagtattgt aaaaaagaaa
    40321 aggagtttca ttatgaagaa aaagaatggt aaagctaaaa agtggcaact gtatgcagca
    40381 atcggtgctg cgagtgtagt tgtattgggt gctgggggga ttttactctt tagacaacct
    40441 tctcagactg ctctaaaaga tgagcctatt catcttgttg ttgccaagga aggaagcgtg
    40501 gcctcctctg ttttattgtc agggacagta acagcaaaaa atgaacaata tgtttatttt
    40561 gatgctagta agggtgattt agatgaaatc cttgtttctg tgggcgataa ggtcagcgaa
    40621 gggcaggctt tagtcaagta cagtagttca gaagcgcagg cggcctatga ttcagctagt
    40681 cgagcagtag ctagggcaga tcgtcatatc aatgaactca atcaagcacg aaatgaagcc
    40741 gcttcagctc cggctccaca gttaccagcg ccagtaggag gagaagatgc aacggtgcaa
    40801 agcccaactc cagtggctgg aaattctgtt gcttctattg acgctcaatt gggtgatgcc
    40861 cgtgatgcgc gtgcagatgc tgcggcgcaa ttaagcaagg ctcaaagtca attggatgca
    40921 acaactgttc tcagtaccct agagggaact gtagtcgaag tcaatagcaa tgtttctaaa
    40981 tctccaacag gggcgagcca agttatggtt catattgtca gcaatgaaaa tttacaagtc
    41041 aagggagaat tgtctgagta caatctagcc aacctttctg taggtcaaga agtaagcttt
    41101 acttctaaag tgtatcctga taaaaaatgg actgggaaat taagctatat ttctgactat
    41161 cctaaaaaca atggtgaagc agctagtcca gcagccggga ataatacagg ttctaaatac
    41221 ccttatacta ttgatgtgac aggcgaggtt ggtgatttga aacaaggttt ttctgtcaac
    41281 attgaggtta aaagcaaaac taaggctatt cttgttcctg ttagcagtct agtaatggat
    41341 gatagtaaaa attatgtctg gattgtggat gaacaacaaa aggctaaaaa agttgaggtt
    41401 tcattgggaa atgctgacgc agaaaatcaa gaaatcactt ctggtttaac gaacggtgct
    41461 aaggtcatca gtaatccaac atcttccttg gaagaaggaa aagaggtgaa ggctgatgaa
    41521 gcaactaatt agtctaaaaa atatcttcag aagttaccgt aatggtgacc aagaactgca
    41581 ggttctcaaa aatatcaatc tagaagtgaa tgagggtgaa tttgtagcca tcatgggacc
    41641 atctgggtct ggtaagtcca ctctgatgaa tacgattggc atgttggata caccaaccag
    41701 tggagaatat tatcttgaag gtcaagaagt ggctgggctt ggcgaaaaac aactagctaa
    41761 ggtccgtaac caacaaatcg gttttgtctt tcagcagttc tttcttctat cgaagctcaa
    41821 tgctctgcaa aatgtagaat tgcccttgat ttacgcagga gtttcgtctt caaaacgtcg
    41881 caagttggct gaggaatatt tagacaaggt tgaattgaca gaacgtagtc accatttacc
    41941 ttcagaatta tctggtggtc aaaagcaacg tgtagccatt gcgcgtgcct tggtaaacaa
    42001 tccttctatt atcctagcgg atgaaccgac aggagccttg gataccaaaa caggtaacca
    42061 aattatgcaa ttattggttg atttgaataa agaaggaaaa accattatca tggtaacgca
    42121 tgagcctgag attgctgcct atgccaaacg tcagattgtc attcgggatg gggtcatttc
    42181 gtctgacagt gctcagttag gaaaggagga aaactaagat gcagaatctg aaatttgcct
    42241 tttcatctat catggctcac aagatgcgtt ctttgcttac tatgattggg attattatcg
    42301 gtgtttcatc agttgttgtg attatggctt tgggtgattc cctatctcgt caagtcaata
    42361 aagatatgac taaatctcag aaaaatatta gcgtcttttt ctctcctaaa aaaagtaaag
    42421 acgggtcttt tactcagaaa caatcagctt ttacggtttc tggaaaggaa gaggaagttc
    42481 ctgttgaacc gccaaaaccg caagaatcct gggtccaaga ggcagctaaa ctgaagggag
    42541 tggatagtta ctatgtaacc aattcaacga atgccatctt gacctatcaa gataaaaagg
    42601 ttgagaatgc taatttgaca ggtggaaaca gaacttacat ggacgctgtt aagaatgaaa
    42661 ttattgcagg tcgtagtctg agagagcaag atttcaaaga gtttgcaagt gtcattttgc
    42721 tagatgagga attgtccatt agtttatttg aatctcctca agaggctatt aacaaggttg
    42781 tagaagtcaa tggatttagt taccgggtca ttggggttta tactagtccg gaggctaaaa
    42841 gatcaaaaat atatgggttt ggtggcttgc ctattactac caatatctcc cttgctgcga
    42901 attttaatgt agatgaaata gctaatattg tctttcgagt gaatgatacc agtttaaccc
    42961 caactctggg tccagaactg gcacgaaaaa tgacagagct tgcaggctta caacagggag
    43021 aataccaggt ggcagatgag tcccttgtat ttgcagaaat tcaacaatcg tttagtttta
    43081 tgacgacgat tattagttcc atcgcaggga tttctctctt tgttggagga actggtgtca
    43141 tgaacatcat gctggtttcg gtgacagagc gcactcgtga gattggtctt cgtaaggctt
    43201 tgggtgcaac acgtgccaat attttaattc agtttttgat tgaatccatg attttgacct
    43261 tgttaggtgg cttaattggc ttgacaattg caagtggttt aactgcctta gcaggtttgt
    43321 tactgcaagg tttaatagaa ggtatagaag ttggagtatc aatcccagtt gccctattta
    43381 gtcttgcagt ttcggctagt gttggtatga tttttggagt cttgccagcc aacaaggcat
    43441 cgaaacttga tccaattgaa gcccttcgtt atgaatgaga tcaacaagat ggacactcgt
    43501 ctatctt
//
LOCUS       DAVDWT010000018        42892 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVDWT010000018 DAVDWT010000000
VERSION     DAVDWT010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42892
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.018"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(58..1068)
                     /locus_tag="VUW50_001107"
     CDS             complement(58..1068)
                     /locus_tag="VUW50_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HET7983013.1"
                     /translation="MSFLSKNGAGILVCLLISIVSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSYKQLDVGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSSDGFALFAGTAVNDTSSVTATASAWDSLYQTNTLESATIVKLT
                     RTLAIIPITLFLSYWQSREQKNKQGLQLKKVFPLFILHFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIIMAMSTIGLKTNLVAMVKSSGKSIVLGAVCWIAIILTSLGMQTLIG
                     IF"
     gene            1217..2386
                     /locus_tag="VUW50_001108"
     CDS             1217..2386
                     /locus_tag="VUW50_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7983014.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEVFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2383..3153
                     /gene="recO"
                     /locus_tag="VUW50_001109"
     CDS             2383..3153
                     /gene="recO"
                     /locus_tag="VUW50_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET7983015.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3150..4142
                     /gene="plsX"
                     /locus_tag="VUW50_001110"
     CDS             3150..4142
                     /gene="plsX"
                     /locus_tag="VUW50_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET7983016.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     PYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEGRDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4148..4381
                     /locus_tag="VUW50_001111"
     CDS             4148..4381
                     /locus_tag="VUW50_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7983017.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQSVGDVVKIIQGK"
     gene            4427..4718
                     /locus_tag="VUW50_001112"
                     /pseudo
     CDS             4427..4718
                     /locus_tag="VUW50_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            4920..5150
                     /gene="blpU"
                     /locus_tag="VUW50_001113"
     CDS             4920..5150
                     /gene="blpU"
                     /locus_tag="VUW50_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HET7983018.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
     gene            5865..8018
                     /locus_tag="VUW50_001114"
     CDS             5865..8018
                     /locus_tag="VUW50_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HET7983019.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMIFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            8031..9380
                     /locus_tag="VUW50_001115"
     CDS             8031..9380
                     /locus_tag="VUW50_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET7983020.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGTSLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            9550..10257
                     /locus_tag="VUW50_001116"
     CDS             9550..10257
                     /locus_tag="VUW50_001116"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET7983021.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            10314..14039
                     /locus_tag="VUW50_001117"
     CDS             10314..14039
                     /locus_tag="VUW50_001117"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET7983022.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDVTELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            14132..15574
                     /locus_tag="VUW50_001118"
     CDS             14132..15574
                     /locus_tag="VUW50_001118"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET7983023.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGVEWI
                     CDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            15793..16815
                     /locus_tag="VUW50_001119"
     CDS             15793..16815
                     /locus_tag="VUW50_001119"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET7983024.1"
                     /translation="MANKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLVSSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            16812..17357
                     /gene="purN"
                     /locus_tag="VUW50_001120"
     CDS             16812..17357
                     /gene="purN"
                     /locus_tag="VUW50_001120"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET7983025.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            17441..17950
                     /locus_tag="VUW50_001121"
     CDS             17441..17950
                     /locus_tag="VUW50_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET7983026.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            17975..19522
                     /gene="purH"
                     /locus_tag="VUW50_001122"
     CDS             17975..19522
                     /gene="purH"
                     /locus_tag="VUW50_001122"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET7983027.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAANENQIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            19644..20906
                     /gene="purD"
                     /locus_tag="VUW50_001123"
     CDS             19644..20906
                     /gene="purD"
                     /locus_tag="VUW50_001123"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET7983028.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRTAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDGKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            21309..21797
                     /gene="purE"
                     /locus_tag="VUW50_001124"
     CDS             21309..21797
                     /gene="purE"
                     /locus_tag="VUW50_001124"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET7983029.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            21784..22875
                     /gene="purK"
                     /locus_tag="VUW50_001125"
     CDS             21784..22875
                     /gene="purK"
                     /locus_tag="VUW50_001125"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET7983030.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            22885..23112
                     /locus_tag="VUW50_001126"
     CDS             22885..23112
                     /locus_tag="VUW50_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HET7983031.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            23175..24473
                     /gene="purB"
                     /locus_tag="VUW50_001127"
     CDS             23175..24473
                     /gene="purB"
                     /locus_tag="VUW50_001127"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET7983032.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(24528..28466)
                     /gene="strH"
                     /locus_tag="VUW50_001128"
     CDS             complement(24528..28466)
                     /gene="strH"
                     /locus_tag="VUW50_001128"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET7983033.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTTTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMDFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQEEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(28782..29498)
                     /locus_tag="VUW50_001129"
     CDS             complement(28782..29498)
                     /locus_tag="VUW50_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7983034.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            29846..31633
                     /locus_tag="VUW50_001130"
     CDS             29846..31633
                     /locus_tag="VUW50_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET7983035.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            31630..32106
                     /locus_tag="VUW50_001131"
     CDS             31630..32106
                     /locus_tag="VUW50_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET7983036.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            32134..33039
                     /locus_tag="VUW50_001132"
     CDS             32134..33039
                     /locus_tag="VUW50_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET7983037.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            33026..33841
                     /locus_tag="VUW50_001133"
     CDS             33026..33841
                     /locus_tag="VUW50_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET7983038.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            33848..34252
                     /locus_tag="VUW50_001134"
     CDS             33848..34252
                     /locus_tag="VUW50_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7983039.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            34546..35712
                     /locus_tag="VUW50_001135"
     CDS             34546..35712
                     /locus_tag="VUW50_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET7983040.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVAKPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            35828..36865
                     /locus_tag="VUW50_001136"
     CDS             35828..36865
                     /locus_tag="VUW50_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HET7983041.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFEFNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGIPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            37072..37926
                     /locus_tag="VUW50_001137"
     CDS             37072..37926
                     /locus_tag="VUW50_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7983042.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     RGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSKLEITDSDGI
                     VTRIRLAR"
     gene            38076..38333
                     /locus_tag="VUW50_001138"
     CDS             38076..38333
                     /locus_tag="VUW50_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET7983043.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVKSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(38375..38737)
                     /locus_tag="VUW50_001139"
     CDS             complement(38375..38737)
                     /locus_tag="VUW50_001139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983044.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDGTDEVSSKHCFEVVDRTDEVS
                     NHIYGKVKLTWFEEIFEKYKSPHNKTHITKVLMNCTPKVRQKKSNFLGAVHSTPDTMR
                     FFNFKDFLQLLLTPIKLF"
     gene            complement(38791..39087)
                     /locus_tag="VUW50_001140"
     CDS             complement(38791..39087)
                     /locus_tag="VUW50_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7983045.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPDAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            39309..40073
                     /locus_tag="VUW50_001141"
     CDS             39309..40073
                     /locus_tag="VUW50_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HET7983046.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            40686..42065
                     /locus_tag="VUW50_001142"
     CDS             40686..42065
                     /locus_tag="VUW50_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET7983047.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLV
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            42079..42744
                     /locus_tag="VUW50_001143"
     CDS             42079..42744
                     /locus_tag="VUW50_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET7983048.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQFWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
BASE COUNT        11891 a         8271 c        10145 g        12585 t
ORIGIN      
        1 aacatatacc tacggcaagg cgacgttgac gtggtttgaa gagattttcg aagagtatta
       61 gaaaatgccg ataagggtct gcataccaag gctggtgagg atgatggcaa tccagcagac
      121 ggctccgaga acaatggatt ttccactgga tttgaccata gcgaccagat tagttttgag
      181 accgatggta ctcatggcca tgataatgag gaatttagag agttgtttga gaggggtaaa
      241 gaaactacta gacacaccga gagaggtcag aagggtggtt aggagcgatg caaggatgaa
      301 gtgaaggata aaaagtggga agactttttt cagttgtaag ccttgcttat ttttttgctc
      361 gcgactttgc cagtaggaga gaaagagagt gatggggatg atagctaggg ttcgcgtgag
      421 tttgacaatg gttgcggatt cgagggtatt ggtctggtag agactgtccc aagcgctagc
      481 tgtggcggtt acagaggaag tatcattgac cgcagttcct gcaaagaggg cgaagccgtc
      541 actggataga tgaagccagg tgccgagggt tggaaagatg agcgcagcca agacattgaa
      601 gaaaaagata acggagatgg cttgggctac ttccttttcc ttagcatgga taacaggcgc
      661 tgtcgctgca atggcagaac ccccacagat agaagaacct actccaatca aggtagccag
      721 ttttgtatcc agggcaaaga agcgctggaa gaggtaggca acaatcaagg ctattgaaat
      781 ggtggaaagg atgacaggga gtgaagattg cccaactgcg aagacttgag agatattgag
      841 accaaaacca agcaagataa cggcatactg gagcaatttc ttggaactaa aggtcaatcc
      901 aacatccagt tgtttataag acgagagaaa gggatgtagg agcatgccca taaaaatcgc
      961 aaaaacgggc gcgccaatca cagggaagaa tcctcctaag taccaagata cgatagaaat
     1021 gagaaggcag accaagatgc ctgctccatt ttttgataga aatgacataa aaacctccaa
     1081 atagaatctg ttaccattat agacctgtaa acaggaaaag taaaatagaa agtggaaagc
     1141 tattctctaa tgtatttttg cggtcggggg gcttttgtag tataatagag atatgttttg
     1201 aaagtaggag gtatctatgg acttaactaa gcgctttaat aaacagttag ataaaattca
     1261 agtttcgttg attcgtcagt ttgaccaggc tatttcggag attcctgggg tcttgcgttt
     1321 gaccttaggg gaacctgatt ttacaacgcc agaccatgtc aaggaggcgg ccaagcgagc
     1381 gattgatcag aaccaatcct actatacagg gatgagtggt ctgctgactc tacgtcaggc
     1441 ggccagtgat tttgttaagg aaaagtacca actggactat gctcctgaaa atgaaatctt
     1501 ggttacaatt ggggcgacag aggctttatc tgcgactttg acggctattt tggaagaggg
     1561 agacaaggta cttttgccag ctcctgctta tccaggctat gaaccgattg ttaacttagt
     1621 tggggcagaa attgttgaga ttgatacgac tgaaaatggt tttgtcttga ctcctgagat
     1681 gttggagaag gccattttgg agcagggtga taagctcaag gcggttattc tcaactatcc
     1741 agccaatccg acaggaatta cctacagtcg agagcagtta gaggccttgg cagctgtttt
     1801 acgcaagtac gaagtttttg ttgtctgtga tgaggtttac tcagaattga cctacacagg
     1861 cgaagcccat gtgtctctag gaacgatgtt gagagaccag gctattatta tcaatggttt
     1921 gtctaaatcg catgccatga caggttggcg tttggggctg attttcgctc ctgcgacctt
     1981 cacagcccag ttaatcaaga gtcaccagta cttggtcact gccgcaaata ccatggcgca
     2041 acatgctgcg gtagaagcct tgacggctgg taaaaacgat gcggagccca tgaagaagga
     2101 atatatccaa cgtcgggact atatcatcga aaaaatgact gctcttggtt ttgagattat
     2161 caaaccagac ggtgccttct atatttttgc taaaattcca gcgggctaca atcaagactc
     2221 ctttgctttt ctgaaggatt ttgctcagaa gaaggccgtt gcctttatcc ctggtgcagc
     2281 ctttggacgt tacggggaag gctacgtccg cctatcttat gcagccagca tggagactat
     2341 caaagaagcc atgaaacgac ttgaggagta catgagagaa gcatgattca gtctatcacg
     2401 agtcaaggct tggtgcttta caatcgcaat tttcgtgagg atgacaagct cgtcaaaatt
     2461 tttacagagc aggttggcaa acgcatgttt tttgtcaaac acgctggtca gtctaagctg
     2521 gcgcctgtta ttcagccctt ggtgctggca cgatttctct tgcgaatcaa tgatgacgga
     2581 ctcagttaca tcgaagacta tcatgaggtc atgacttttc ccaagattaa tagtgacctc
     2641 tttgtcatgg cctatgcgac ctatgtggca gctcttgcag atgctagttt gcaggacaat
     2701 cagcaggatg ctcccttgtt tgcttttttg caaaagactt tggagttgat ggaagcaggc
     2761 ttggattatc aggttttgac caatattttt gaaattcaaa ttttgactcg atttggaatc
     2821 agcctcaatt ttaatgagtg tgtcttctgc catcgggttg gtcaggcttt tgacttttct
     2881 ttcaaatatg gagcctgcct ctgtccagag cattatcatg aggataagag acgttgtcat
     2941 ctcaatccca atatccccta tctgctcaat caatttcaag ctattgattt tgagactttg
     3001 gagaccattt cgctcaagcc tggaatcaag caagagctac gccaatttat ggatcaatta
     3061 tatgaagagt acgttgggat tcacctaaaa tcaaagaaat ttattgattc cctagcagac
     3121 tggggacaat tactaaaaga ggaaaagaaa tgaaaaaaat cgcagtagat gccatggggg
     3181 gcgattacgc acctcaggcc attgttgagg gtgtcaatca agccctatct gacttttcag
     3241 atatcgaggt tcaactttac ggagatgaag ctaaaatcaa gccctatctg acagcgacag
     3301 agcgcgtcag cattatccat acggatgaga agattgattc ggatgatgaa cctacgagag
     3361 ctattaggaa taagaaaaat gccagtatgg tattggcagc caaggctgtc aaagatggtg
     3421 aagcagacgc tgtcctttcg gctgggaata caggtgcctt gttggcagca ggattcttca
     3481 tcgtgggtcg tatcaagaat atcgaccgtc ctggactcat gtctaccttg cctaccgttg
     3541 atggaaaagg ttttgacatg ctagaccttg gtgccaatgc agaaaataca gcccagcacc
     3601 tccatcaata tgcggttcta ggttccttct atgctaaaaa tgtccgtggc attgcgcaac
     3661 cacgcgttgg tttgctcaac aacggaacag agagtagcaa gggcgacccg cttcgtaagg
     3721 aaacttatga attactggcg gctgatgaaa gtttgaactt tatcggaaac gtggaagggc
     3781 gtgatttgat gaatggcgtt gcagatgttg ttgtggcaga tggtttcacg ggaaacgctg
     3841 tgctcaaatc catcgaaggg acagctatgg gaatcatggg cttgctcaag acagctatta
     3901 caggtggtgg tcttcgagcg aaactaggtg ccctccttct caaggacagc ctcagtggtt
     3961 tgaaaaaaca gctcaattat tcagatgttg gtggagcggt cttgtttggt gttaaggcac
     4021 ctgttgtcaa gactcatggc tcaagcgatg ccaaggctgt ttatagtacg attcgtcaga
     4081 tccgtaccat gctagaaaca gacgtggttg cccagactgc gcgtgaattt tcaggagaat
     4141 aaaagagatg acagaaaaag aaatttttga ccgtattgtg accattatcc aagagcgaca
     4201 gggagaggac tttgtcgtga cagaatcctt gagtctgaaa gacgatttgg atgcggattc
     4261 tgttgacttg atggagttta tcttgactct ggaagatgaa tttagtatcg aaatcagcga
     4321 tgaggaaatt gaccaactcc aaagtgtagg agatgtggtt aaaatcattc aaggaaaata
     4381 gcaatcggag ttccaagtca acggaagtag atggttttta gaaatgagaa atatcggaca
     4441 agctggtaaa atcttggctg acagtggtta tcaagggctc atgaagatat atcctcaagc
     4501 acaaacttcc acgtaaatcc agcaaactca agccgctaac agttgaagat aaagcctgta
     4561 atcatgcgct atctaaggag agaagcaagg ttgagaatat ctttgccaaa gtaaaaacgt
     4621 ttaaaatgtt ttcaacaacc tatcgaaatc atcgtaaacg cttcggatta cgaatgaatt
     4681 tgattgctgg tattatcaat catgaactag gattctagtt ttgcaggaag tctaatagta
     4741 aaaagtgatt agaaaacatc ttttttaaaa atagagatga ttttgaaaca aaaaagctaa
     4801 ttcaagacgt ttcgattcca attcaagatt tggatgaaaa aaattaatag atactgttat
     4861 actaaacttg tcaagtttgt aacaagacaa atattaaaaa taaaaaagag gtattcgtta
     4921 tgaatacaaa aaagatgtca caatttgaaa ttatggatac tgagatgctt gcttgcgttg
     4981 aaggtggcgg atgcaattgg ggagattttg ccaaagcagg tgttggagga ggagcagtac
     5041 gaggtcttca gctaggaatt aaaacaagaa catggcaagg tgcagcaact ggtgctgcgg
     5101 gaggagctat acttggaggt gtggcctatg cagcgacatg ttggtggtaa ttatggattt
     5161 taaaagtttt attattggtt tagtagttgg tatatttggt ccttatatgg atgatttaat
     5221 tagaaaaaaa tttttaaagt cttcggagaa gaaaacagaa aaatctgtta aaaaataatc
     5281 aaaactataa atgatgaatc tgaatcaaaa ttattttgcg catgtaaaga ggagtcttat
     5341 agtaacgagt caaaaaagga gtaactatga atcgtaattt agaacggtgt tatctattct
     5401 gactaggaat agatcatacc agaggtagct tagaaatagc agggacatta gaaattgaag
     5461 taataaatag gatgtcgtaa gtgttactat caatgattta tttgtttcaa gcttgcctag
     5521 ggtgacagta aaaaatcaat ttcctttcaa tagcatattt ttagtgggca ggactcttgt
     5581 tctgcctatt tttttatcca aaaagtgcag ttgggaggga gataggctca tttgggaagg
     5641 aagtccagtt tttgtttagt gattggggta agatagttgt tatcagatga gtttatactc
     5701 ttcgaaaatc aaattcaaac cacgtcaacg tcgccttgcc gtatatatgt gactgacttc
     5761 gtcagtccta tctacaacct caaaacagtg ttttgagcag cctgcggcta gtttcctagt
     5821 ttgctctttg attttcattg agtattaggg aaaaggagat gaatatgaaa tttgggaaac
     5881 gtcactatcg tccgcaagtg gatcagatgg actgcggtgt agcttcatta gccatgattt
     5941 ttggctacta tggtagttat tattttttgg ctcacttgcg agaattggct aagacgacca
     6001 tggatgggac gacggctttg ggcttggtca aggtggcaga ggagattggt tttgagacgc
     6061 gagccattaa ggcggatatg acgctttttg acttgccgga tttgactttt ccttttgttg
     6121 cccatgtgct taaggaaggg aaattgctcc actactatgt ggtgactggg caggataagg
     6181 atagcattca tattgccgat ccagatcccg gggtgaagtt gactaaactg ccacgtgagc
     6241 gttttgagga agaatggaca ggagtgactc tttttatggc acctagtcca gactataagc
     6301 ctcataagga acaaaaaaat ggtctgctct cttttatccc tatattagtg aagcagcgtg
     6361 gcttgattgc caatatcgtt ttggcaacac tcttggtaac cgtgattaac attgtgggtt
     6421 cttattatct gcagtctatc attgatacct atgtgccaga tcagatgcgt tcgacactag
     6481 ggattatttc tattgggcta gtcatcgtct acatcctcca gcaaatcttg tcttacgctc
     6541 aggagtatct cttgcttgtt ttggggcaac gcttgtcgat tgacgtgatt ttgtcctata
     6601 tcaagcatgt ttttcacctc cctatgtcct tctttgcgac acgcaggaca ggggagatcg
     6661 tgtctcgttt tacagatgct aacagtatca tcgatgcgct ggcttcgacc atcctttcga
     6721 ttttcctaga tgtgtcaacg gttgtcatta tttcccttgt tctattttca caaaatacca
     6781 atctcttttt catgacttta ttggcgcttc ctatctacac agtgattatc tttgccttta
     6841 tgaagccgtt tgaaaagatg aatcgggata ccatggaagc caatgcggtt ctgtcttctt
     6901 ctatcattga ggacatcaac ggtattgaaa ctatcaagtc cttgaccagt gaaagtcagc
     6961 gttaccaaaa aattgacaag gaatttgtgg attatctgaa gaaatccttt acctatagtc
     7021 gagcagagag tcagcaaaag gctctgaaaa aggttgccca tctcttgctt aatgtcggca
     7081 ttctctggat gggggctgtt ctggtcatgg atggcaagat gagtttgggg cagttgatta
     7141 cctataatac cttgctggtt tactttacca atcctttgga aaatatcatc aatctgcaaa
     7201 ccaagcttca gacagcgcag gttgccaata accgtctaaa tgaagtgtat ctagtagctt
     7261 ctgagtttga ggagaagaaa acagttgagg atttgagctt gatgaaggga gatatgacct
     7321 tcaagcaggt tcattacaag tatggctatg gtcgagacgt cttgtcggat atcaatttaa
     7381 ccgttcccca agggtctaag gtggcttttg tggggatttc agggtcaggt aagacgactt
     7441 tggccaagat gatggttaat ttttacgacc caagtcaagg ggagattagt ctgggtggtg
     7501 tcaatctcaa tcagattgat aaaaaagccc tgcgccagta catcaactat ctgcctcaac
     7561 agccctatgt ctttaacgga acgattttgg agaatcttct tttgggagcc aaggagggga
     7621 cgacacagga agatatctta cgggcggtcg aattggcaga gattcgagag gatatcgagc
     7681 gcatgccact gaattatcag acagaattga cttcggatgg ggcagggatt tcaggtggtc
     7741 aacgtcagag aatcgctttg gcgcgtgctc tcttgacaga tgcgccggtc ttgattttgg
     7801 atgaggcgac tagcagtttg gatattttga cagagaagcg gattgtcgat aatctcattg
     7861 ctttggacaa gaccttgatt ttcattgctc accgcttgac tattgctgag cggacagaga
     7921 aggtggttgt cttggatcag ggcaagattg tcgaagaagg aaagcatgct gatttgcttg
     7981 cacagggagg cttttacgcc catttggtca atagctagaa agaggagagg atgaaaccag
     8041 aatttttaga aagtgcggag ttttataatc gtcgttacca taatttttcc agtagtgtga
     8101 ttgtacccat ggcccttctg ctcgtgtttt tacttggctt tgcaactgtt gcagagaagg
     8161 agatgagttt gtccactaga gctactgtcg aacccagtcg tatccttgca aatatccagt
     8221 caactagcaa caatcgtatt cttgtcaatc atttggaaga aaataagctg gttaagaagg
     8281 gggatctttt ggttcaatac caagaagggg cagagggtgt ccaagcggag tcctatgcca
     8341 gtcagttgga catgctaaag gatcaaaaaa agcaattgga gtatctgcaa aagagcctgc
     8401 aagaagggga gaaccacttt ccagaggagg ataagtttgg ctaccaagcc acctttcgcg
     8461 actacatcag tcaagcaggc agtcttaggg ctagtacatc gcaacaaaat gagaccatcg
     8521 cgtcccagaa tgcagcagct agccaaaccc aagccgaaat cggcaacctc atcagtcaaa
     8581 cagaggctaa aattcgcgat taccagacag ctaagtcagc tattgaaaca ggtacttcct
     8641 tggccggtca gaatctagcc tactctcttt accagtccta caagtctcag ggcgaggaaa
     8701 atccccaaac taaggttcag gcagttgcac aggttgaagc acagatttct cagttagaat
     8761 ctagtcttgc tacttaccgt gtccagtatg caggttcagg tacccagcaa gcctatgcgt
     8821 cagggttaag cagtcaattg gaatccctta aatcccaaca cttggcaaag gttggtcagg
     8881 aattgaccct tctagcccag aaaatcttgg aggcagagtc aggtaagaag gtacagggaa
     8941 atcttttaga caaggggaaa attacggcaa gtgaggatgg ggtgcttcat cttaatcctg
     9001 agaccagtga ttctagcatg gttgcagaag gtgccctact agcccaactt tatccatctt
     9061 tggaaagaga aggtaaagcc aaactcacag cttatctaag ttcaaaagat gtagcaagaa
     9121 tcaaggtcgg tgattctgtt cgctatacta cgactcatga tgccgggaat caacttttcc
     9181 tagattctac tattacaagt attgatgcga cagctactaa gactgagaaa gggaatttct
     9241 ttaaaatcga ggcggagact aatctaactt cggagcaggc tgaaaaactt aggtacgggg
     9301 tggaaggccg cttgcagatg attacgggca agaaaagtta tctacgttat tatttggatc
     9361 aatttttgaa caaagagtaa tgttcgtgtt tttagagtta aataattttt aaactgtgag
     9421 aaagattctt cttgcagttt tttctttaca atttttggaa aacacctgct atttattcgg
     9481 ttaaattctt gtattttttg gttttttgtg gtaaaatgtg ctcaagtaat acgaaaggcg
     9541 aactttaaaa tgtcaaaaca attgatctat tcgggaaaag ctaaagatat ctatacaact
     9601 gaggatgaaa atcttattat ttcaacttac aaggaccagg cgactgcttt caacggtgtc
     9661 aagaaggagc agattgcagg taagggagtc ttgaataatc agatctcatc ttttattttt
     9721 gagaaattaa atgcggctgg tgtggcgact cactttgtgg agaaactttc agacacggaa
     9781 caactcaata aaaaggttaa gattattcct ttggaagtcg tgctccgcaa ctatactgct
     9841 ggttcctttt caaaacgttt tggtgtggat gagggaatcg ccttggagac tccgattgtc
     9901 gaattttact acaaaaatga tgatttggat gatccattta tcaatgatga gcatgtgaaa
     9961 ttcctacaga ttgcggatga ccagcagatt gcctacttga aggaagaaac gcgtcgtatc
    10021 aatgaactat tgaaagtctg gtttgctgag attgggctta aattgattga ctttaagcta
    10081 gagttcggtt ttgacaagga tggcaagatt atcttggcag acgaattttc accagataac
    10141 tgccgcttgt gggatgctga tggcaatcac atggataagg atgttttccg tagaggattg
    10201 ggagaactaa ccgatgttta cgaaattgtc tgggaaaagt tgcagggttt aaaataacaa
    10261 cctcaaggct gtttgggaat attgcaagag ctgaaataaa ggaataagaa ttgatggata
    10321 aacgtatttt tgttgaaaaa aaggctgatt ttcaggtcaa gtcagagagt ttggttagag
    10381 agctccagca caacttggga ctgtcaagct tgaaaagtat tcgtattgtg caagtatatg
    10441 atgtatttga cttggctgag gacttgtttg cacctgcaga gaagcacatt ttctctgagc
    10501 aggtaaccga ccatgtttta gatgaagtat ctgtgcaggc ggatcttgct aactatgctt
    10561 tctttgccat tgaaagtcta ccagggcagt ttgaccagcg tgcagcttcg tcacaggaag
    10621 ccttgctttt gttgggaagt tcgagtgacg tgacagtcaa cacagcccaa ctttacttgg
    10681 tgaataaaga tattgatgtg actgagttgg aagctgtcaa aaactacctg ctcaatccag
    10741 ttgattctcg tttcaaggat atcacgacag ggattgccaa gcaggagttt tcagagtcag
    10801 acaagaccat tccaaaattg actttctttg aaagctatac agcagaagac tttgctcgct
    10861 acaaggccga gcaagggatg gccatggaag tggatgattt gctctttatc caagactact
    10921 ttaagtcaat cgggcgcgtg ccaactgaga ctgaactcaa ggttttggac acttactggt
    10981 ctgaccactg ccgtcatacg acttttgaga cagagttgaa acacatcgac ttttcagctt
    11041 ctaaatttca aaagcaattg cagtcaacct atgacaagta tattgccatg cgcgaggaat
    11101 taggtcggtc tgaaaaacca caaaccttga tggatatggc gactattttc ggtcgttatg
    11161 agcgtgctaa tggacgattg gatgatatgg aagtctctga cgaaatcaat gcctgctcag
    11221 ttgaaattga agtggacgtt gatggtgtca aggaaccttg gctcctcatg tttaaaaatg
    11281 aaacccacaa ccatccaaca gaaattgagc catttggtgg agcggctacc tgtatcggtg
    11341 gagctattcg tgatccgttg tcaggccgtt cctatgttta ccaagccatg cgtatttcag
    11401 gtgctggtga tattacagca ccgatttcgg aaactcgtgc tgggaaattg ccacaacaag
    11461 tcatttctaa aacagcagct catggttatt cttcatatgg taaccagatt gggcttgcca
    11521 caacctacgt tcgtgaatac ttccacccag gctttgtagc taaacgtatg gaacttggtg
    11581 ccgttgttgg tgcggctccc aagggcaacg ttgtccgtga aaaacctgaa gcaggtgatg
    11641 tgatcatcct tctcggaggc aaaacaggtc gtgatggtgt cggtggtgcg acgggctctt
    11701 ctaaggttca aacagttgag tctgtagaga ctgctggtgc tgaggttcaa aaaggaaatg
    11761 ccatcgaaga acgcaagatt cagcgcctct tccgtaatgg caatgtcact cgtctgatca
    11821 agaagtccaa tgactttggg gcaggcggcg tctgtgtggc tatcggtgaa ttggcagacg
    11881 gtcttgaaat cgacctcaac aaggtgcctc ttaaatacca gggcttgaat ggtacagaaa
    11941 ttgccatctc tgaatcacaa gaacggatgg cggtcgtggt tcgtcctgaa gatgtggata
    12001 ccttcgttgc cgaatgtaac aaagaaaata ttgatgctgt tgtggtggcg acagtaactg
    12061 aaaaaccaaa tcttgtcatg cactggaatg gtgagacaat cgttgacttg gagcgtcgtt
    12121 tccttgacac caatggtgtg cgcgtggttg tcgatgccaa agttgtggac aaggatgtca
    12181 aactcccaga agagcgtcaa acatctgctg aaacactgga atcagatacc cttacggttc
    12241 tatctgacct caaccatgca agtcaaaaag gattacagac tatctttgac tgctctgttg
    12301 gacgctcaac ggttaatcac ccacttggtg gtcgttacca actcacacca actgaggcat
    12361 ctgtgcagaa attgccagtt caacacggtg tgactcatac tgcgtcggtc attgctcaag
    12421 gtttcaaccc atatgtagct gaatggtctc cataccacgg tgctgcttat gcggttatcg
    12481 aagcaactgc tcgtttggtg gctgctggtg ccaactggtc taaggctcgt ttctcttacc
    12541 aagaatattt cgagcggatg gacaagcaag cagagcgttt cggtcagcca gtagctgctc
    12601 ttctaggttc tattgaagca caaattcagc ttggcttgcc atctatcggt ggtaaggact
    12661 ccatgtctgg tacctttgaa gaattgaccg ttccgccaac cttggttgcc tttggggtga
    12721 cgacggcaga tagccgtaag gtgctctctc cagaatttaa agctgttggg gaaaatatct
    12781 actacatccc aggtcaagcc ctctctgcag agattgattt tgacttgatt aagaaaaatt
    12841 ttgctcagtt tgaagcaagc caagctgacc ataaagtgac gtctgcatca gctgtcaaat
    12901 acggtggtgt agttgaaagt ttggctcttg ctacctttgg aaactatatt ggtgcagagg
    12961 tgaccttgcc tgaacttaaa acagctttga cagctcaatt aggcggcttt gtcttcacat
    13021 ctcctgaaga aattgctgga gtagagaaga ttggacaaac gaaagcagac tttacactga
    13081 ctgtcaacgg tgtgaagcta gatggacaca agcttgacag tgcatttcaa gggacattgg
    13141 aagaagttta cccaacagaa tttacccaag cgaaagaact agaagaagta ccagctgtgg
    13201 catcagatgt tgtgattaaa gccaaagaaa aggttgaaaa acctgtggtt tacatcccag
    13261 tctttccagg aaccaactca gaatatgatt cagctaaggc cttcgaaaaa gaaggtgcag
    13321 aggtcaattt ggtgccattc gtgaccttga atgaagaagc tattgtcaag tcagttgaaa
    13381 ctatggttga caatatcgac aagactaata ttctcttctt tgccggtgga ttctcggctg
    13441 cggatgaacc agatggttca gctaagttta tcgtcaatat cctgcttaat gaaaaagtgc
    13501 gtgtggctat tgatagcttt atcgcccgtg gtggcttgat tatcggtatt tgtaatggat
    13561 tccaagcctt agtcaaatcg ggtctcctac catacggaaa ctttgaagat gctaacagta
    13621 ctagtccaac cctcttctac aatgatgcca accaacacgt ggccaagatg gtggaaactc
    13681 gtattgccaa taccaactca ccatggttgg ctggtgtgca agtgggcgat atccacgcta
    13741 ttcctgtttc gcacggtgaa gggaagtttg tcgtgacggc tgaggaattt gcagagctcc
    13801 gtgacaatgg acaaattttc agccaatacg ttgactttaa cggtaaacca agtatggatt
    13861 ctaagtacaa tccgaatggt tctgtccatg ccatcgaagg aattaccagc aagaatggtc
    13921 aaatcatcgg taagatgggc cactcagaac gttatgagga tggtcttttc caaaatatcc
    13981 caggcaataa agaccaacac ctgttcgcat cagcggttaa acatttcact ggaaaataag
    14041 acttacagat tttctaatag atagtatcag taatgtaaaa gtcatgtaaa tctagctctt
    14101 gatgattaca aatgaaaatt aggtataaaa aatgacatac gaagtaaaat ctcttaatga
    14161 agaatgtggt gttttcggta tttggggaca tccagatgct gctaagttga cctattttgg
    14221 actccacagt cttcaacacc gtggtcagga gggggcagga atcctctcca atgatcaagg
    14281 acaactgaag cgccatcgtg acatggggct tttatcagaa gttttcagaa atccagccaa
    14341 tttggataaa ttgacaggag ctggtgcgat tgggcatgtg cgttatgcga ctgctggcga
    14401 agcttctgtt gataacatcc aacccttcct cttccgtttt cacgatatgc agtttggttt
    14461 ggctcataat ggaaatctga ccaatgcagc ctctctcaag aaagaactgg aacaaagagg
    14521 agcaattttc agcgcgactt cggactctga aatcttggct cacctcattc gtcgcagtca
    14581 taatcctagc ctgatgggca aaatcaagga agcgctcagc cttgtcaaag gtggttttgc
    14641 ctatatcttg ctgtttgagg acaagttgat tgcggctctt gaccctaatg gctttcgtcc
    14701 gctttctatc gggaaaatgg ccaacggagc ggtggttgtt tcctctgaaa cctgtgcttt
    14761 tgaggtcatt ggtgtcgagt ggatttgtga tttgaagcca ggtgagattg tgatcattga
    14821 tgacgagggc attcagtatg acagctatac agatgatacc cagttggcga tttgttctat
    14881 ggagtatatc tactttgccc gccctgattc taatatccac ggtgtcaatg tccatacggc
    14941 acgtaagaga atgggagcgc aattggcgcg agaatttaag catgaggcag atattgtagt
    15001 tggtgtgccc aattcttccc taagcgcggc tatgggattt gcggaagaat caggcttacc
    15061 aaatgaaatg ggtctgatca aaaaccaata cacccagcga acttttatcc aaccgactca
    15121 agaattgcgg gagcaaggag tgcggatgaa actgtctgct gtttcgggtg ttgtcaaagg
    15181 caaacgtgtg gtcatggtgg atgattccat tgtacgtgga acaacctctc gtcgtatcgt
    15241 tcagctcttg aaagaagcgg gtgcgactga ggttcacgtt gccattggaa gtcctgcact
    15301 agcgtatcca tgtttctacg ggattgatat ccagacccgt caggagctga ttgcagccaa
    15361 tcatacggtc gaagaaactc gccaaatcat tggtgcggac agtctgactt atctttcaat
    15421 tgatggcttg attgagtcga ttggtatcga aacagatgcg ccgaacggtg gtctctgtgt
    15481 cgcttacttt gacggtgact acccaacgcc tctctatgac tacgaagaag actatcgtag
    15541 aagtttggaa gaaaagacca gtttttacaa gtaggcgaca gattctccat taaagaaaag
    15601 gaaaaaacaa atgacaaata aaaatgcata tgcctcacgt ctcactactg actaaaggct
    15661 taagcattta gtcagtagac gctttgtcct ataggatcaa agctagagcc ctgactagta
    15721 tttttagata aaaagatggt ttatctaaaa atacgtcgca gtctttctca aaaaaagaaa
    15781 aggaaaaata aaatggcaaa taaaaatgcg tacgctcaat ctggtgtgga tgttgaagcg
    15841 ggttatgaag ttgttgaacg gattaaaaag cacgtggctc gtacggagcg tgcaggtgtc
    15901 atgggagctc ttggtggctt tggtggtatg tttgaccttt ccaagactgg ggttaaagaa
    15961 cccgtcttga tttcagggac tgacggtgtc ggaaccaagc tcatgttggc tatcaagtac
    16021 gacaagcacg ataccatcgg gcaggactgt gtggccatgt gtgtcaacga catcattgct
    16081 gcaggtgcgg aacccctcta ttttctcgac tacgtagcga cagggaagaa tgaaccagct
    16141 aagctagaac aagtggttgc tggtgtggca gaaggttgtg tgcaggctgg tgctgccctc
    16201 atcggtgggg aaacggctga aatgccgggc atgtacggcg aagacgacta tgacttggct
    16261 ggttttgcgg tcggtgtggc tgaaaaatct caaatcattg acggttcaaa ggtggtagag
    16321 ggagatgttc ttctcggact tgtttcaagt gggattcact caaatggtta ctctttggtt
    16381 cgtcgtgtct ttgcggatta cacaggtgag gaagtcctac cagaattgga aggcaagaaa
    16441 cttaaggaag ttctacttga gccgactcgt atctatgtca aggctgtctt gccgctcatc
    16501 aaagaagagt tggtcaacgg cattgcccac atcacaggtg gtggctttat cgaaaatgtc
    16561 cctcgtatgt ttgcagatga cctagctgct gaaattgatg aaagcaaagt tccagtgctt
    16621 ccaattttca aagcccttga aaaatatggt cagattaaac acgaagaaat gtttgaaatc
    16681 ttcaatatgg gtgtgggact tatgttggcg gtcagccctg aaaatgtaga gcgtgtaaaa
    16741 gaattgttgg atgaagcagt ctatgaaatt ggtcgcatcg tcaagaaaga aaacgaaagt
    16801 gtcattatca aatgaaaaaa atagcggttt ttgcctctgg taatggctca aattttcagg
    16861 tgattgccga agaatttcca gtggagtttg tcttttcaga ccatcgtgat gcctatgtgc
    16921 ttgagcgtgc aaagcagctc ggcgttctgt cctatgcttt tgaactcaag gagtttgaga
    16981 gcaagacaga ctacgaagca gcccttgtcg aactcttgga agaacaccag attgacttgg
    17041 tttgcctagc aggctacatg aaaatcgttg gaccaacctt attgtcggct tatgaaggtc
    17101 ggattgtcaa cattcatcca gcctacttgc cagaatttcc aggagctcat gggattgagg
    17161 atgcttggaa tgctggcgtg ggtcagtctg gtgtgaccat tcactgggtg gattcgggtg
    17221 tggatacagg ccaggtcatc aaacaggttc gtgtgccacg actagctgat gataccattg
    17281 acagatttga agctcgcatc catgaagcag agtacaggtt gtatccggaa gtagtgaagg
    17341 ctctatttac agattgactt tttgatgatt catatgatat ctttgatttt aaattggagt
    17401 cagtgtttgt tgaagacggc ttcaaacgga ggtatttgta atgttagaat ctaaaaaaac
    17461 aactcgatat gtattttatg tctatctgat gttattaact tggggaatct tatttaagtt
    17521 tgaaacaaat cctgaattta tagcattttt cttagctcca aggtatatca attggattcc
    17581 attttcagaa ccactaatag tcgatggaaa aattgttttt gctgaaatgt tatttaatct
    17641 gatttccttt attccattag gtgtttgttt ccctttgata aaaactaatt tatctagttt
    17701 aagaatagtc gggacaggtt tcttgattag tttattgttt gagtgcttac agtatatttt
    17761 agcaataggt ataacagata taacggattt gactttaaat acgctaggtg tctgtgtagg
    17821 cttactgatt tatcaaattt ttataagagt gttcaaatca cagactagaa aatggatcaa
    17881 tatcttaggt atgcttagcc ttggttttgc ttatcttgtt ttactgttac tgcatttaat
    17941 tggtgtttaa ctaatgatta aaaaggagaa tataatgact aaacgcgtct taatcagcgt
    18001 ctcagacaaa gcgggcattg ttgaatttgc ccaagaacta aaaaaacttg gttgggagat
    18061 tatctcgaca ggtggaacta aggttgccct tgataatgct ggggtggaga ccattgcgat
    18121 cgatgatgtg acgggcttcc cagagatgat ggatggccgt gttaagactc ttcatccaaa
    18181 tatccacggg ggtcttctcg ctcgtcgtga tttggatagc cacttggaag cagccaatga
    18241 gaaccaaatt gagcttattg accttgtagt ggtcaacctt tacccattta aggaaactat
    18301 ccttaaacca gatgtgactt atgctgatgc agttgaaaat atcgatattg gtgggccgtc
    18361 tatgcttcgt tcagcagcga aaaatcatgc cagtgttaca gttgtggtag atcctgctga
    18421 ctacgctgtg gttttggacg aattggcagc aaacggcgaa acctcttatg aaactcgcca
    18481 acgtttagca gccaaagtat ttcgtcacac agcggcttat gacgccttga ttgcagaata
    18541 cttcacagct caagtgggtg aaagcaagcc tgaaaaactc actttgactt atgacctcaa
    18601 gcaaccaatg cgttacggtg agaatcctca acaagacgcg gacttttacc agaaagcttt
    18661 gcctacagat tactctattg cttcagccaa acagctcaac gggaaagaat tgtcatttaa
    18721 taatatccgt gatgcagatg ctgctatccg tatcatccgt gacttcaaag atagtccaac
    18781 cgttgtggct ctcaaacaca tgaatccatg tggaattggt caagctgatg acatcgagac
    18841 tgcttgggac tacgcttatg agtctgaccc agtatctatc tttggtggga ttgtcgtcct
    18901 caaccgtgag gtggatgctg cgacagctga gaagatgcac ggcgttttcc tcgaaatcat
    18961 cattgcacca agctatacgg atgaagcgct agctattttg atcaataaaa agaaaaactt
    19021 gcgtatcctt gccttgccat ttaatgctca agaggctagc gaagtggaag cagaatacac
    19081 aggtgtagtc ggtggacttc tcgtgcaaaa tcaagacgtg gtcaaggaaa gcccagctga
    19141 ctggcaagtg gtgaccaaac gtcagccaac tgagacagaa gcgactgctc ttgagttcgc
    19201 ttggaaggct atcaagtacg tcaaatcaaa tggtattatc gtgaccaacg accacatgac
    19261 acttggtgtt ggtccaggtc aaaccaaccg tgtggcttct gttcgccttg ccattgacca
    19321 agccaaagat cgtctgaacg gggcggtcct tgcttcagat gccttcttcc catttgcgga
    19381 taacgtggaa gaaatcgcca aagcaggaat taaggccatc atccagcccg gtggctctgt
    19441 ccgtgaccaa gaatccatcg aagcagcgga taaatacggc ttgactatgg tctttacagg
    19501 tgtgagacat tttagacatt aagaagataa aagggaagaa aacagtttct ttcctttttt
    19561 ggcttaaaat gctaactgaa acaagattaa aacgaacttt tttgatataa tgttggtaaa
    19621 taattcgcaa aagaggttga ggaatgaaac tgcttgttgt cggttctggt ggtcgtgagc
    19681 atgcgattgc taaaaagtta cttgaatcaa aagacgtgga aaaagtcttt gtagctcctg
    19741 ggaatgatgg gatgactctg gatggtttgg aattggtaaa tatctctatt tccgaacatt
    19801 ctaaattgat tgacttcgca aagaccaatg atgttgcttg gacctttatc ggtccagatg
    19861 atgcccttgc tgctggtatc gtggatgatt ttaaccaagc tgggcttaag gcctttggtc
    19921 cgactaggac tgcagcggag ctggagtggt ccaaggattt tgccaaggaa atcatggtca
    19981 aatatggtgt ttcgacagca gcctatggca cattttcaga tttcgaggaa gcaaaggcct
    20041 atatcgaaaa gcatggtgcg cctatcgtag tcaaggcgga tggcttggcg cttgggaagg
    20101 gtgtcgtcgt tgctgagacg gttgagcaag cggtcgaagc cgctcatgag atgcttttgg
    20161 acaataaatt tggtgactca ggtgcgcgcg tggttattga ggaattcctt gaaggagagg
    20221 aattttcact ctttgccttt gtcaatggtg ataagttcta catcatgcca acggctcagg
    20281 accacaaacg tgcctacgat ggcgacaaag ggcctaacac gggtggtatg ggtgcctatg
    20341 cgccagttcc acacttacca cagagtgtag ttgatacagc ggttgacacc attgtcaagc
    20401 cagttctaga agggatgatt aaagaaggtc gcccttatct gggagttctt tacgcagggc
    20461 ttatcctgac agctgatgga ccgaaagtca ttgagttcaa cgctcggttc ggagatccag
    20521 aaactcagat tatcttgcct cgcttgacct ctgactttgc tcaaaatatc acagatattc
    20581 tggatggcaa ggagccaaat atcatgtgga cggacaaggg tgtgactctg ggtgtggttg
    20641 tcgcatccaa gggctacccg ctagactatg aaaggggcgt tgagttgcca gcaaagacag
    20701 aaggcgatgt catcacctac tatgcagggg ctaagtttgc ggaaaatagc agagcactgc
    20761 tctcaaacgg cggacgagtt tatatgctcg ttaccacagc agataccgtc aaagaagccc
    20821 aagccagcat ctaccaagaa ctatcccaac aaaaaataga aggactcttc taccgaacag
    20881 atatcggaag caaggcaatt aagtaaagat ataagaataa cgcgccgtag tcgccaaaca
    20941 cgataatggt cgtcgtggtg aaaagaccag aacagtgaat gttctggtca gggggaaact
    21001 tggagacctt aggctcaaag tttaggaatg aaaccgaagg tttgcttccg cctccatcac
    21061 ctaagaccat tatcaaaaag aaaaataaaa attcacaaaa tacgttaatg atcgtatggt
    21121 ttgcgagcgt tagcgagcta atatagaaca atcaccgccg ttgtgaaaga acgattggat
    21181 gataatccaa tcgttcaggg aaattggaag accttgggtt tccaatttag gcatgagaca
    21241 cctttggtgg ctgctgccgt ccctcacaag ctaaggtgat tgttgaaaaa gaggaaaaag
    21301 gagaagaaat gaaaccagta atttccatca tcatgggctc aaaatccgac tgggcaacca
    21361 tgcaaaaaac agcagaagtc ctagaccgct tcggtgtagc ctacgaaaag aaagttgttt
    21421 ccgcacaccg tacaccagac ctcatgttca aacatgcaga agaagcccgt agtcgtggca
    21481 tcaagatcat catcgcaggt gctggtggcg cagcgcattt gccaggcatg gtagctgcca
    21541 aaacaaccct tccagtcatt ggtgtgccag tcaagtctcg tgctcttagt ggagtggatt
    21601 cactctattc tatcgttcag atgccgggtg gggtgcctgt tgcgaccatg gctatcggtg
    21661 aagctggagc gactaacgca gctctctttg ccctccgtct cctctctgta gaagataagt
    21721 ccattgcgga tgcacttgcc aactttgctg aagaacaagg aaaaatcgca gaggagtcgt
    21781 caaatgagct catctaaaac aatcggaatt atcggtggcg gtcaactggg tcagatgatg
    21841 gccatttctg ctatctacat gggccacaag gttatcgcgc tggatcctgc ggcggattgc
    21901 ccggcctctc gcgtggcgga aatcattgtg gcaccttata acgatgtgga cgccctccgt
    21961 cagttggcag accgttgcga tgtcctcact tacgagtttg aaaatgtcga cgctgacggt
    22021 ttggatgccg ttatcaagga tggacaactc cctcaaggaa cagatctgct ccgcatttcg
    22081 caaaatcgta tttttgaaaa ggactttttg tcaaacaagg ctcaagtcac tgtggcaccc
    22141 tacaaggtcg tgacttctag cctagacttg gcagatatcg acttgtcgaa aaactatgtc
    22201 ctcaagactg cgactggtgg ctacgatggt catggacaaa aggttattcg ttcagaagca
    22261 gacttggaag cagcctatgc gctagcagac tcagcagact gcgtcttgga agaatttgtc
    22321 aactttgacc ttgagatttc tgtcatcgtg tcaggaaatg gcaaggaggt gacgtttttc
    22381 ccagttcagg aaaatatcca ccgcaacaat atcctgtcta agaccatcgt accagcccgc
    22441 atttctgaaa gtctagtaga caaggctaaa gctatggcag tgcgaatcgc agaacaactc
    22501 aacttgtctg gaactctctg tgtggaaatg tttgcgactg atgatgacgt tattgtcaat
    22561 gaaatcgccc cacgaccaca taactctggg cactattcta ttgaagcctg tgatttctct
    22621 cagtttgaca cccatattct cggtgttctc ggagcaccat taccagtcat caaactccat
    22681 gcgccagccg ttatgcttaa tgtcctcggt cagcatgtcg aggctgctga gcaatatgtg
    22741 acagaaaatt caagcgccca cctccacatg tatggtaaaa tagaagcgaa gcacaaccgc
    22801 aagatgggac atgtgacctt gtttagtgat gtaccggata gtgtggatga gcttggggaa
    22861 gggattgatt tttaggacaa gtctatgata caaattatcg ttaatacatt tattgaaaag
    22921 gataagactg gagcagttgt tgaagtgttg tatgccagtg ctgaccaaga taaggtacaa
    22981 gctaaatatg aagaactagc tgcacaatac cccgaaaatt atttagctat ctatgatgta
    23041 ccgctggata cggatttgaa tacactagat cattacccgt ctgtgtttat tgaaaaagag
    23101 gagtttgagt agaaatcttg gtttacctag atagcttatt cccaacagct taagaagaaa
    23161 ggaaaaatta acacatgatc aaccgttact ctcgccctga gatggcgaat atttggagtg
    23221 aagaaaataa ataccgtgct tggcttgagg tggaaatcct ctctgacgag gcatgggctg
    23281 agttggggga aatccctaag gaagatgtgg ctttgattcg caagaaggcg gactttgaca
    23341 tcgaccgtat tttggaaatt gagcaggaga cgcgccacga tgtggtggct ttcacgcgtg
    23401 cggtttctga gactcttggt gaagagcgca agtgggttca ctatgggtta acttctaccg
    23461 acgtggtgga tactgcttat ggttacctct acaagcaggc caacgacatc atccgtcgtg
    23521 accttgaaaa cttcactaat atcatcgctg acaaggccaa ggagcacaag ttcaccatca
    23581 tgatggggcg tactcatggt gtgcacgctg agccgacaac ctttggtctt aaattggcaa
    23641 cttggtacag cgaaatgaaa cgcaatatcg agcgtttcga gcatgcagct gctggtgtag
    23701 aagctggtaa gatttctggt gcggttggga actttgccaa tatcccacca tttgtagagg
    23761 agtatgtctg cgataaactt ggcatccgtg cccaagaaat ctctacacaa gtccttcctc
    23821 gtgaccttca cgctgagtac tttgcggttc ttgccagcat tgcgacttca atcgaacgta
    23881 tggcgactga gattcgtggt ctacaaaaat ctgagcaacg cgaagtagaa gagttctttg
    23941 ctaaagggca aaaagggtct tcagcaatgc ctcacaaacg caacccaatc ggttctgaaa
    24001 atatgactgg tctggcgcgt gtcattcgtg gtcacatgat tacggcttat gaaaacgtcg
    24061 ctctctggca cgaacgcgat atttctcact catcagctga gcgtatcatc acaccagata
    24121 cgaccatttt gattgactac atgctcaacc gttttggaaa tatcgtcaag aacttgacag
    24181 tcttcccaga aaatatgatc cgaaacatga actcgacttt tggtcttatc tttagccaac
    24241 gggctatgtt gacattgatt gaaaaaggca tgacccgtga gcaagcctat gacttggtgc
    24301 aaccaaaaac agcctactct tgggacaacc aagtagactt taaaccactt cttgaggcag
    24361 attcagaagt aacatcacgt ctcactcaag aagaaatcga tgaaatcttc aacccagttt
    24421 attacaccaa acgagtggat gatatctttg aacgtcttgg actaggtgat taattaaaaa
    24481 ataaacagcg agcttcaatc tcgctgttta ttttttatcg aaaagactta gtcttctttt
    24541 cttttagtga gtccataggc tgctagtgtg gacatgagtc ctgcgactac tagtcctgca
    24601 gaatcgtgag ttcctgtttc aggaagtttt ttctctgtta ccacaggagc tggatcttga
    24661 ggaagagctt tgctttcctc agcaggagca gttgatggag ctggttggct tgggatttct
    24721 agttttggtt tttcttcagc aatagcggct tgtccgtttt catcgcctac atgtgttacc
    24781 atagttccga cttcgactat ttgagtaacg gcttcctgtg ctacgacact atttacaagt
    24841 gttttcactt ccttaccatc ggcagaagtg ctcacagagt agaagttgct acgatgtcca
    24901 ttgacgccct tagtaatgac ttgtgttttt cctttgagta agagtggatt ttcacgagtc
    24961 actgtggtaa atggaatttc ttctttttgg atatccagtc taggttttac ctcagtagtt
    25021 ggtgcaagac cactttcatc acccttgtga gttacaggag cgccaacttc aaccacttgg
    25081 tttataactt ctttggttac ctggctatca aggactgttt ctgttgtttt tccattttca
    25141 gtgagtacag agatgtaatg agttcgttca cctttgactc ctgctgtgat aatattttct
    25201 tgaccagctg ggaggttagg attttctttc ttgataactt caaatggaat ttcttcagtt
    25261 cttgtgatga gttctggtct ggtttcaaca ttggcagcca cttcattttc atctaggctt
    25321 cctgaatgag ttgcagctgg tttgaggcct tgaagagcgg cttttaggtt ggctacaagt
    25381 gtgtcaagct cagcttgttt attacggttg aggttgtaat ttagagctgt tttagctgcg
    25441 tcaagggcct caagactttc tttactatat ccttctaagt ttgtaggaat tttagctaat
    25501 tcttcgcgga gagcattata attagcacgg aagtagtctt tgttgtggtc tgcaaaggca
    25561 gtcatgagtt caaagatttc ctcttccttg tattcagcgc ttggtctatc tgcccagatt
    25621 gaaagcatac ttccgactgt tggaagatct acttcaggat atttggtaga agctagttga
    25681 ttgaatggaa ctttaccaga attttcaact gcttttgaaa gagggtaagc ttcctcttgt
    25741 ttgtgattgc ctataacata ataccagtca ccgttggtat tcaagaattt atagcctttg
    25801 cttgctaggt attgaggtga tgcgaggtta tatccccacc agcctttaga ccagtaagaa
    25861 atcaagacat ctttgtcaaa ctgaacatcg tccttgtctt catagtagaa gccatcgttg
    25921 aaggccattg gttgaagccc tctttctttg gccatagctg cgagggtgtt ggcatattcg
    25981 gcaaatttgc catagagttg ataccacttg aggtagtacc agccttgggc actagtcgca
    26041 tcgttggcgt attcgtcagt accaaagttg aaaatctttg ttttacctgc aaagaagtcc
    26101 atgtatttac cgatgagggc ttttacaaag tccatcgctt cttcgttttt caagtccata
    26161 gttgtttttg aaactttatc aaagtgggct tgaggatttt taatacctaa tttttccatg
    26221 gcaaccagca tagcatccat gtgacctgga ctgttaatag ctgggatgag accgatgtcc
    26281 ttagatttag cgtattcaat tagctctgtc acttctgcct gtgttagtgc agtaccgttt
    26341 ggatcgtcgt agtaagcttt agttccttcg ataatagctt ttttaacgtc atcactagca
    26401 taggtttttc cgttggcagt aatggtcata tcattgagta gaaagcgaag tccgtcattt
    26461 cctagaagga gatggacatc agaatatccg agctcactgg ccttgtctac gatgcgtttg
    26521 agctggttca gagtaaagta tttgcgtcca gcatcgattg agattacctt gtttttggca
    26581 agtttttcaa cctcacgttt agcttcttct tctttttgag cttcaggcgt gagggtcaag
    26641 ttgttgacag tttcttgaag tttagcaatg gcttgatcaa tcgtatcttg ttgggcacgg
    26701 ctaaggttgc tatcgagaga gcgaatagct ttttcagctt cttttacggc cgcgacgctt
    26761 tctgcagtat aacggttcag gtcttttggt acctcgttaa gtgcttgctc tgcagattca
    26821 taatcagctg cgaagtattc agcgttggca tttgcaaaat gacgcatgag tttgaagagg
    26881 cgtgatggtg aataacgtgc agatggagtg tcagcccaag cagctaccat accaccgatg
    26941 attgggatat cagctccttc tgtttttggt acagaagtga ttggtgtgtt tttaatacca
    27001 ttgagcccct gatcgagatt gtaccagcct tggccatcag cgtttcgtcc aagaacgtag
    27061 taccaagcat cattggtatt aaggatttgg tgaccttttt cagctagtag tttagaagaa
    27121 gcgacatcgt agcctcccca accaccagtc cacatagaaa cgatgatgtc tttgtcaaaa
    27181 ctaccaaagc ttgtgtcgct attgtagtag ataccgtcgt taaaggccat tggtttgaga
    27241 ccgtgcgatt ttacaatacg agcgaggtca ttggcgtagg caataaattt ttcatagcct
    27301 tttacagggt agccttcgtt tggatagtat ttatcagctt gaagcacact ccaaccttta
    27361 gcatctgtcg catcattggc atattcatca agtccgatgt tgaagatttc agtctttttc
    27421 gcgaaataag cagcatactt gtcgataagg gcttttgtaa aagcgacagc ttgttcgttg
    27481 tcaagatcga cagtacgggc tgatttcttc ccaaaatagc taaagttagg gttttggatt
    27541 cccaattctt tcatggcatt gagaatcgca tccatgtgtc caggactatt tactgtcgga
    27601 atgagaccga tacctttatc tttggcatag ttaatcagat ctgtcatttg actttctgtt
    27661 aagtgattgc cgtttggatc gttgtaataa tcatttgtac ctttttcaat ggcgcgtttg
    27721 acatcgtcac tggcataggt cttgccgtta gctgtgatgc tcatatcgtc caacatgaaa
    27781 cggagtccat catttccgac taataggtgt aaatcagtgt agccataatg tttcgcttta
    27841 tcgatgattt ccttgagctg ttctggtgag aaatatttac gtccagcatc aatagaaaca
    27901 attttctttt tcgctagttt ttcatttaca gttgcagcac gttcctttcc tgcctctgtt
    27961 tccggtttgt cagcctctgc tttcgcttca tcttttttag ctggtttatc cttgtcagtc
    28021 ttgtctgtat ttgactcttt agaatcaacc tctttcgctt cttccttttt agggctagct
    28081 tcttctgcct ttttattagc agtttctttt tcagcagaag ttggagttac cacctctgct
    28141 ttatcactag gagttgaact aacttcctct tgtggttttt cttctgtttt tggaagacta
    28201 gctaccttat cagtagctgg agtttctgtt tctacagttt ttggagcttc tggttgaagc
    28261 tctgctttag gtgtttcctc agtccgattt tcggatgatt gaggggaatc agaaactgta
    28321 tggatggtcg gttggttttc tgtagtagta gtagtaactc catcggctgc aacagtctgt
    28381 gcttggaagg caaatccaat tagaacagaa gctgctccta cagcgtattt acgaatagaa
    28441 aaacgctgtt gtttttcatg tttcattgca aaacctcctg attgcattgt tatattgata
    28501 gcgattatat aaatcaacgc ccttatttta tttcttatat taacgagagt caagaggaga
    28561 tgacaaaaaa ctataataag tataaaaaaa tataaaattt aaacttaaga tttcagattg
    28621 gtcggaaaaa atgcatatat atatctaata taatttttgg ttctatttct ataaaatatt
    28681 ccacaaatta tagaatcttc caaaaatagg taagcgctac ctttttggtg tggtataata
    28741 agcatagaaa aagcccgagc gattaggctc aggctttctt cttagtgatc acggtcacat
    28801 gagataaatt taatcttgta gtaatcagat cgtttgtaag tttcactgta ttctaaaact
    28861 tggccagttg attcgagttt ggtgatttta gtttgtagga cagtagggaa ttgttcatcg
    28921 actccgagga ctgaagccgc atgttctgga gttggaaaga ctatttcgtt gatttcttca
    28981 aagtgttcat cattcatgtg aatgtggtag tctaacttga aacgattata gatagaacta
    29041 tagtattcaa ggtttggata atttgcgttg atatattgtt ctgggatgta ggatgtatgg
    29101 tagatataaa cgacaccgtt tgattcgcgg atacgttcaa tcttgtagta gaattgatcg
    29161 ccgcgtagac ccaatttttc caagtaaaca agcttgtttc cgcgttcaat tgaaagaaca
    29221 gttaccttat catctttagc attgaagagt tcaatatctg aaaactctac aagcttgtgt
    29281 ttgcgtgcac gtgaaacgaa ggttcctttt ccttgttggc ggacaatata gccatctttg
    29341 gcaaggtcgt ttaaggcgcg aacaactgtg atagagctga catcgtacat tgaaatgagt
    29401 tctgcttcag tgtaaaattt atctccactg ctaaactgcc cagagatgat tttatttttt
    29461 aattcgtctt ttatgtattg atatttggga attgccatat ttttacctct attttccttc
    29521 tccatagaaa attttaccat aaaagcgaaa aaaatagtag tcttttgaat aaaaaattag
    29581 aataatagtt tgaaaaatta agcgatatag ggattgctca tattttatca atgtattcta
    29641 tacaaaatta ttttaggtcc tatattccga atttaaagag cgtttgaaat gggtctaaaa
    29701 atgtttgaaa ctaattgaat gtgtgtataa tagtaaaaat gcagtaaata tcccgacaat
    29761 ttctattgac aattcaaaca gattggttta taattaatat aacaacaaat gaaagcgcaa
    29821 actttcgcgg tcggaaggta gttttatgac acgatttgag atacgagatg atttctatct
    29881 cgatggaaaa tcatttaaga ttttatctgg tgccattcat tattttaggg ttcctccaga
    29941 ggattggtat cattcgctct ataacttgaa ggctcttggt tttaatacgg tagagactta
    30001 tgttgcttgg aatttacacg agcctcgtga aggtgagttt cattttgaag gtgatctgga
    30061 tttagagaaa tttctccaaa tagcgcagga tttgggtctc tacgcaattg tgcgtccgtc
    30121 tccatttatc tgtgcggaat gggaattcgg tggcttacca gcttggctct tgaccaagaa
    30181 catgcgaatt cgctcatccg acccagcata tatcgaggca gttggtcgct actatgatca
    30241 gttattgcca agactggtgc ctcgtttgtt ggacaatggt ggcaatattc tcatgatgca
    30301 ggttgaaaat gagtatggtt cttacggaga agataaggct tacctgagag cgattcgaca
    30361 gctaatggaa gagtgtggcg taacctgtcc cctctttaca tcagatggtc catggcgagc
    30421 tactctgaaa gctggaacct taattgaaga ggacctcttt gtaacaggaa actttggttc
    30481 taaggcacct tacaactttt cgcagatgca ggaattcttt gatgaacatg gtaagaaatg
    30541 gccactcatg tgtatggagt tctgggatgg ttggttcaat cgctggaaag aaccgattat
    30601 cacacgggat cctaaggaat tggcagatgc agttcgagag gttttggaac aaggctctat
    30661 caatctttac atgttccacg gtggtacaaa ctttggtttc atgaatggtt gctcggctcg
    30721 aggaactttg gacctgccac aagttacgtc ttatgattac gatgcccttc tggatgaaga
    30781 aggaaatcca actgctaaat atcttgcagt caagaagatg atggcaacac atttttcaga
    30841 gtatccgcag ttggaaccac tctacaaaga gagtatggag ttggatgcta ttccactagt
    30901 tgaaaaagtt tctttatttg aaaccttaga tagcttgtca agtcctgtag aaagtctcta
    30961 tcctcaaaag atggaggagc tgggacaaag ttatggctac ctactttatc gaacagaaac
    31021 aaactgggat gcagaagaag aaagacttcg tatcattgat ggtcgagata gggcccagct
    31081 gtatgtcgat ggtcagtggg ttaaaactca atatcagaca gagattgggg aagatatttt
    31141 ttatcaaggt aaaaagaaag ggctatctag gttagatatc ttgatagaaa atatggggcg
    31201 tgtcaactat gggcataagt tcttagcgga tacgcaacgt aagggaattc ggacaggggt
    31261 ctgtaaggat ctgcatttct tactaaactg gaaacactat ccactcccac tagacaatcc
    31321 tgagaaaatt gatttttcaa aaggatggac tcaaggacaa ccagcctttt acgcttatga
    31381 ctttacagtc gaagagccaa aagatactta cctagacttg tctgagtttg gtaagggggt
    31441 tgcctttgtc aatgggcaga atctaggacg tttttggaac gttggcccaa ctctctcact
    31501 ttatatccct catagctatc tcaaggaagg tgccaaccgc atcattatct ttgaaacaga
    31561 aggtcaatat aaagaagaga ttcatttaac tcgtaaacct acactaaaac atataaaggg
    31621 ggaaaactta tgacaattgt aggatgccgt attgatggac gtttgatcca cggacaagta
    31681 gccaatcttt gggctggaaa actaaatgtt tcacgcatta tggttgtaga cgacgaagtt
    31741 gtcaacaacg atattgaaaa gagtggtttg aaacttgcga caccaccagg tgtgaaattg
    31801 agtattttgc cagttgagaa agctgcagcc aatattcttg ctggcaaata cgatagccaa
    31861 cgtctcttta tcgtggctcg taaaccagac cgcttccttg gtttggtaga agcaggtgta
    31921 ccacttgaaa cccttaatgt tgggaatatg tctcaaacac cagaaactcg ttctattaca
    31981 cgttctatca acgtagtaga caaggatgtg gaagacttcc acaaactggc agaaaaaggt
    32041 gttaaactta ctgctcagat ggttccaaat gatccaattt cagacttttt gagcttatta
    32101 aaataggaaa aaaattttta ggaggtcatt gttatgatac aatggtggca aattttactt
    32161 ctcactttgt actcagctta tcaaatctgt gatgagttga cgatcgtttc atctgcaggt
    32221 tcccctgtat ttgctggttt cattactggt ttaatcatgg gagatgtgac tactggttta
    32281 cttatcggtg gtaacttgca actgttcgtt cttggggttg gtactttcgg tggtgcttct
    32341 cgtatcgacg caacttctgg tgcggttctt gcgacagcct tctctgtttc acaaggaatt
    32401 gatgcaccgc ttgccattac tacaatcgct gtaccagtag cagctctctt gacttacttc
    32461 gacgttcttg gtcgtatgac tactaccttc ttcgctcacc gtgtggatgc tgcaatcgaa
    32521 cgctttgact ataaaggtat tgaacgcaac tacttgcttg gtgcgattcc gtgggctcta
    32581 tctcgtgccc ttccagtctt ctttgccctt gcttttggtg gtgcctttgt acaatcagta
    32641 gtagacttcg ttgaagccta caaatgggtt gcagatggct tgacacttgc aggacgtatg
    32701 cttccaggtc ttggatttgc aatcttgctt cgttaccttc cagttaaacg taaccttcac
    32761 taccttgcta tgggatttgg tttgacagct atgttgactg ttctttactc atatgtaaca
    32821 ggtcttggtg gcgctgttgc tggtatcgta ggtactcttc ctgctgaagt tgctgaaaaa
    32881 attggtttcg tgaacaactt caaaggtttg tctatgattg gtatttctat cgtaggtatt
    32941 ttccttgcag tgcttcactt caaaaatagc caaaaagtag ctgtagcagc accttctaca
    33001 ccatcagaaa gtggggaaat cgaagatgac gaattctaat tacaaactta caaaagaaga
    33061 ttttaatcaa atcaacaaac gtagcttgtt tactttccaa ttaggttgga actacgaacg
    33121 tatgcaagct tctggttacc tttacatgat cttgcctcag ttgcgtaaaa tgtatggtga
    33181 tggaactcct gaattgaaag aaatgatgaa agttcatact caattcttca atacttcacc
    33241 attcttccat accattatcg ctggttttga ccttgccatg gaagaaaaag atggtgtagg
    33301 ttcaaaagac gccgttaacg gtatcaagac aggtttgatg ggaccattcg ctcctcttgg
    33361 ggatacaatc tttggttcac ttgtacctgc tatcatgggg tcagtcgcag caactatggc
    33421 tatcgctggc caaccttggg ggatcttcct ttggattgca gttgcagtag cgtatgacat
    33481 cttccgttgg aaacagttgg aatttgctta caaagaaggg gttaacctta tcaacaacat
    33541 gcaaagtacc ttgacagctt tgattgacgc tgcatctgta cttggtgtct tcatgatggg
    33601 tgctcttgta gcaacagtga ttaactttga aatttcttac aagttgccaa tcggtgaaaa
    33661 gatgattgat ttccaagaca tcttgaacca aatcttccca cgtttgcttc cagcaatctt
    33721 tactgccttt atcttctggt tgcttggtaa aaaaggtatg aactctacta aagctatcgg
    33781 tattattatc gtacttgctt tggctctttc tgcccttggt cactttgcac ttggaatgta
    33841 attccttatg actaaatcat taattttggt gagccatggt cgcttctgtg aggagcttag
    33901 aggtagcaca gaaatgatta tgggcccaca agacaacatt tacacagtag ctcttcttcc
    33961 agaagatggc ccagaagaat ttactgctaa atttgaagct gttattgaag ggttggatga
    34021 tttcctagtc tttgcggatc ttctcggtgg gacaccttgt aatgtggtga gtcgcttgat
    34081 catggaaggt cgtgatattg acctttacgc agggatgaat cttccaatgg tgattgaatt
    34141 tatcaatgcg agccttacag gcgcagatgc ggactacaag agccgtgctg cagaaagcat
    34201 tgtgaaagtt aatgacctgt tagcgggctt cgatgatgac gaagatgaat aatactcttc
    34261 gaaaatctct tcaaactacg tcaacgtcgc cttgccgtag gtatatgtta ctgacttcgt
    34321 cagtcttatc cggcaacctc aaaacggtgt tttgagctga cttcgtcagt tctatctgca
    34381 acctcaaagc agtgctttga gcagcctgcg gctagtttcc tattttagtt ggaactcgat
    34441 tcaattcatg tgacaacgtg aaaatcgtta gagcatttta tatagaatat acatgggaat
    34501 gtagcttact cccattccca tatttaatag aaaaagagga actcaatgct acattataca
    34561 aaagaagact tgctcgaatt gggtgcagaa atcactacgc gtgaaatcta ccaacagcct
    34621 gatgtatgga gagaagcttt tgaattttat caagcaaaac gtgaagaaat tgcagccttt
    34681 ctacaagaaa tcgctgataa acatgactat attaaggtta tcttgacagg tgctgggact
    34741 tctgcttatg tgggagatac cttgctacct tactttaagg aagtctatga cgaacgcaaa
    34801 tggaatttca atgctattgc gacaacagat atcgttgcca aaccagcaac ctatttgaaa
    34861 aaagatgtgg caactgtcct tgtgtctttt gctcgtagtg ggaactcgcc tgaaagtgtg
    34921 gcgactgttg atttggccaa atccttggtg gatgagcttt atcaagtgac gattacttgt
    34981 gcagcagatg gtaaattggc tcttcaagct cacggtgatg accgtaatct cttgctcttg
    35041 caaccagctg tctctaatga tgctggattt gccatgactt ctagctttac gtctatgatg
    35101 ttgacagctc tcttggtctt tgatcctaca gaatttgctg ttaagtctga acgttttgaa
    35161 gttgtatcta gtcttgcccg taaagtttta gacaaggcag aagatgtcaa agagctcgtt
    35221 gatttagact ttaaccgtgt catctatcta ggcgctggtc ctttctttgg acttgctcat
    35281 gaagctcagc tcaagatttt ggaattaact gctggtcaag ttgcgaccat gtatgaaagc
    35341 ccagttggct tccgtcacgg tccaaaatct cttatcaacg acaatacagt tgttttggtc
    35401 tttggtacaa cgacagacta cactcgtaag tacgacttgg acttggttcg tgaagttgct
    35461 ggtgaccaga ttgctcgtcg tgttgtgctt ttgagtgatc aagcttttgg tcttgaaaat
    35521 gtcaaagaag tggcccttgg ttgtggcggt gtcttgaatg atatttaccg tgtcttccct
    35581 tacatcgttt atgcccaact ctttgcccta ttgacttcac tcaaggtaga aaataaacca
    35641 gatacaccgt ctcctacagg tacagtaaac cgtgtagtac aaggtgtcat aattcacgaa
    35701 tatcaaaagt aagacagtgt ttatgaattc ttgacaagag gatttgtaaa ttatcagata
    35761 aaccatagat tgtcagtacg ctttctatgg tttgtttgct tgagagaaat agtaaaagga
    35821 gaacagaatg aaagcataca cagagcgtgt atttggaaat gttgagggcg aggatgtctt
    35881 ggcctatcga tttgagacag acggtggcta ccaacttgag gttatgactt atggtgcgac
    35941 catcttgcgc tatgtcgcac ctgacaaggc tggaaatttt gccaatgtta tcttgggatt
    36001 tgatgacttt gatagttatg taggcaatag tcccaagcat ggagcaagtg taggtcctgt
    36061 agcgggtcgt attgcaggtg cgacctttga gttcaatggt aagacctatg accttgaggt
    36121 taataatgct agcaactgta atcacagtgg ttcaactggt tgggattcca gcttgtttga
    36181 agttgaagaa gtaagcgatc atggcttgac tctctacaca gagcgtacag atgggacagg
    36241 agggttccct ggaaatctca agatttggat cagttatcac ttggaagaaa ctggtgccta
    36301 tgaaatcagc tacaaggtaa cgaccgatca ggatacgctg gtcaatccaa ccaaccacag
    36361 ctatttcaac ttgtctggtg atttcacgca gacgattgac cgtcatgtct tccaactaaa
    36421 cacagagggc atttactcaa tcgctcctga cggtattcct gccaaaactc cagaagccaa
    36481 ccgtgatgtg gtcaaacaca tctacaatgg taccttgttg aaggatatct ttgcagaaga
    36541 agatgagcaa atccagctgg catcaggttt ggatcaccca tttgcccttc ctgcaggcca
    36601 tgacaatgct ggattccttt atgaccaaaa ttcaggtcgc ttcctgcttt tcaagacaga
    36661 agctccttgc tttgtggtct acacagcaaa ctttgtggat gaaagtgtca tcataggagg
    36721 tcagccaatg ctacagcaca atgggattgc tcttgaagcg caagctttac cagatgccat
    36781 tcacagtgac cttaaaggcc aagtcattct taaagctggt caaaccttca ccagtaagac
    36841 acgttatgaa cttgttgtga aataaaaaag ctggtcaaaa agtctttaaa attaaaataa
    36901 cgcatattgt caggtgatga aaagtttgat aatatgcgtt ttattatgaa atgatttact
    36961 ttatattatc ctgaaatggt gttatttttg agttatctgg cttgactttc ttgatggaaa
    37021 ttatataata gttgtaacaa taataattac aacgagaaaa gaggaccatt tatgacctat
    37081 gaatataaga gtcacattta tttggcagag acagctttaa atgtaaagga tttggccagt
    37141 caaacagcct tttatcagca agttattggt ttagaaatct tatctcaaac tgagacagag
    37201 tccattctag gccttggtgg aaaagtcttg gtacagctga ttcaagcaca agagagcgga
    37261 gaagtgaggg aacattatgg tctttaccat ttggccattc tcttgcccac acgcaaggct
    37321 ttggctgatg tcttgaaaca cctgacagat ttgcaaattc ctcttgtcgg cggtgcagac
    37381 catggttaca gtgaagctat ttacttggaa gatttggagg gaaatggcat tgaactctat
    37441 cgagataagc cagtttccac atgggatatt cgagaagatg gacgcattat cggagtgacg
    37501 gaggctcttg cggcgcagga catttatgag ttgggggaaa gagtagagcc ctttatcctg
    37561 gcagaaggta caagaatggg gcatattcat ctttccgtca aggatagtcg aaagtccagc
    37621 cagttttatc aaaaggtgtt agggctagag aataaattca gtgtgcctgg cgctagttgg
    37681 attgcggctg gagattacca tcatcattta gcagttaacg aatggagagg aaaaggtctg
    37741 gcttcgcgta agcaaggctt accaggttta gcctactatg tcatcgaagt cgcacataaa
    37801 aaagaactgt taacgattgc ccaacgagca caagaagttg acgcaccaat caaatggatg
    37861 acatcgagca aattggaaat cacagactca gatggcatcg tgacccgtat tcgtttagca
    37921 aggtaaaatt aggaattttt aataagttat aagtattgct agtttgcttt ttgaacggtc
    37981 tttggtaaca gaactgcgcg agctggatag gtgagaattt tgacaaagaa gctgattaga
    38041 gatataatga agaaaaatcg agcaaggaaa aagaaatgac aaattcaaag tatattactt
    38101 gtttgaaacg ttcagagggc cagttgtgtg ggattcaaaa gatgattgaa ggagatcgtg
    38161 actgtgctga tattgtgacg caactgacag cagtgaaatc tagtgtagag cgcgtgattg
    38221 agatgataat taccgaaaat cttactgaat gtatcaatca gccactagat gattctgaag
    38281 ctcaaaagga acgcttagaa aaggctatcc gatacttgat taaacggaaa taaatgaatg
    38341 ttttgagacg atatgtgcaa aggtctatta actattagaa tagtttaatg ggagtcaaaa
    38401 gaagttgtaa aaaatcttta aaattaaaaa aacgcatagt atcaggtgtt gaatgtactg
    38461 cccccaaaaa gttagatttt ttctgtctaa cttttggggt gcagttcata agaaccttgg
    38521 taatatgcgt tttattatgg ggagatttat acttttcgaa aatctcttca aaccaggtca
    38581 acttcacctt gccgtagata tggttactga cttcgtcagt tctatccaca acctcaaaac
    38641 agtgttttga gctgacttcg tcagttccat ccacaacctt aaaacagtgt tttgagcagc
    38701 ctgcggctaa cttcctagtt tgcactttaa ttttcattga gtattacttc atcttctcct
    38761 gaaattgagt ttttgcccag ctcctcttga ttattctact tgaccgggcc aagcattcat
    38821 accaccttct acgttggtaa cggtgaggcc ttgggagctg agaaattggc aggtagatgc
    38881 agagcggact cctccttgac aaataacata gtattcatgg tcaggtttga gttctttgta
    38941 gccttgctct aaggtactta atggaagatt tttggcatct ggtgcatgtc ctgcttggaa
    39001 ttcatgtgct tcacggacat cgataagttc tagattttca ttttgatatt ttccataaaa
    39061 gtcagccatg ctgatactag tttccatatt ctactccttc tgggttagtc attttgtata
    39121 gtgaataagc gccgtcaagg ttttggacgg taaatcctgc ttgcttgagg ataggctctg
    39181 cgatatagct gcgcaaacca ctgtggcagc tgacgatgta ggcttgtttt tctcctcagt
    39241 tcatcagctt cctccttgac actttgtcag attttgatac aatagtacaa aattagagga
    39301 ggcaggctat gattcagaaa catgcgattc ctattttaga gtttgatgac aatcctcagg
    39361 cggttatcat gcccaatcac gaggggctgg acttgcagtt gccaaagaag tgtgtttatg
    39421 catttttagg tgaggagatt gaccgctatg caagggaagt aggggcggat tgtgtcggcg
    39481 aattcgtttc tgctaccaag acctatccag tctatgtcat caactacaag ggtgaggagg
    39541 tctgtctggc tcaggctcct gttggctccg ctccagcagc ccagtttatg gattggttga
    39601 ttggctatgg tgtggagcag attatctcta ctgggacctg tggtgtgcta gccgatatag
    39661 aggaaaatgc ctttctagtc cctgttcgcg ctctgcgaga tgagggagcc agttaccact
    39721 atgtggcacc ttgtcgttat atggaaatgc agccagaggc tattgctgct attgaggaag
    39781 ttttggaaga cagagggatt ccttatgaag aagtcatgac ctggacgaca gacggttttt
    39841 accgagaaac ggctgaaaag gtggcttatc gtaaggaaga aggctgtgct gttgtggaga
    39901 tggagtgttc tgctcttgcg gcagtagctc aattgcgtgg ggttctctgg ggtgaattgt
    39961 tgttcacagc agattctcta gcggacttgg accagtacga cagtcgtgac tggggctcgg
    40021 aagcttttaa taaggcgcta gaactgagtt tagcaagtgt tcaccatctt tagttgtact
    40081 ggcaaaggat ttgttttatc ataaaatgtc tagctcatac ttttcaaaaa tatgtttaaa
    40141 cgaggtcacc ttcctcttgt cctaggcatg ttgaggttgg gaaaaatctt taaaatcaga
    40201 aaaacgtatc atatcaggtg atgaaaactt tgatactatg cgttttatgt tggtaagatt
    40261 tagagtgaga tgaaatgata ctcttcgaaa atcttttcaa accaggtcag cttcaccttg
    40321 ccgtaggtat atgttactga cttcgtcagt cttatccggc aacctcaaaa cggtgttttg
    40381 agctgacttc gtcagttcta tccacaacct caaaacagtg ttttgagcaa cctgtgacta
    40441 gctttctaag cgatgccttg gttctcattg cctataatca aaaagagaaa ttttctcctg
    40501 aaaagcatat agagtagctg gcgttaaaag ctcctgtctt gcttttttga cctatagtca
    40561 catctatcaa gtattgttct tgcctaagct atcaataaaa aggtggcatt ttttaggctt
    40621 ggtgttagtg aattttgcct tatcctatct aagtcatttc gagcttttta tggtacaatg
    40681 gaaacatgtt attcaaatta tctaaggaaa aaatagagct aggcttatct cgtttatcgc
    40741 cagcccgtcg tatttttttg agttttgcct tggtcatttt actaggctct cttcttttga
    40801 gcttgccctt tgtccaagtt gaaagctcac gagcgactta ttttgatcat cttttcactg
    40861 ctgtctctgc agtctgtgtg acaggtctct caacccttcc agtagctcac acctataata
    40921 tatggggcca aataatctgt ttgctcttga ttcagatcgg tggtctaggg ctcatgacct
    40981 ttattggggt tttctatatc cagagcaagc aaaagcttag tcttcgtagc cgtgcaacta
    41041 ttcaggatag ttttagttat ggagaaactc gatctttgag aaagtttgtc tattctattt
    41101 ttctcacgac ctttttggtt gagagcttgg gagctatttt gcttagtttt cgccttattc
    41161 ctcaacttgg ctggggacgt ggtcttttta gttccatttt tctagcgatc tcagccttct
    41221 gtaatgccgg ttttgataat ttagggagca ccagtttatt tgcttttcag accgatttac
    41281 tggtcaatct ggtgattgca ggcttgatta ttacaggcgg ccttggtttt atggtctggt
    41341 ttgatttggc tggtcatgta ggaagaaaga aaaaaggacg tctgcacttt catacgaagc
    41401 ttgtattatt attgactata ggtttgttgt tatttggaac ggcaactact ctctttcttg
    41461 agtggaacaa tgctggaacg attggcaatc tccctgttgc cgataaggtt ttagttagct
    41521 tttttcaaac agtgacgatg cgaacagctg gcttttctac gatagattat actcaggctc
    41581 atcctgtgac tcttttgatt tatatcttac agatgtttct aggtggggca cctggaggaa
    41641 cagctggggg actcaagatt acgacatttt ttgtcctctt ggtctttgca cgaagtgagc
    41701 ttctaggctt gcctcatgcc aatgttgcga gacgaacgat cgcgccgcga acggttcaaa
    41761 aatcctttag tgtctttatt atctttttga tgagcttctt gataggattg attctgctag
    41821 ggataacagc caaaggcaat cctcccttta tccacctcgt atttgaaacc atttcagctc
    41881 ttagtacagt tggtgtaacg gcaaatctga ctcctgacct tgggaaattg gctctcagtg
    41941 ttatcatgcc acttatgttt atgggacgaa ttggtccctt gaccttgttt gttagcttgg
    42001 cagattacca tccagaaaag aaagatatga ttcactatat gaaagcagat attagtattg
    42061 gttaagaaag gaaagagcat gtcagatcgt acgattggaa ttttgggctt gggaattttt
    42121 gggagcagtg tcctagctgc cctagccaag caggatatga atattatcgc tattgatgac
    42181 cacgcagagc gcatcaatca gtttgagcca gttttggcgc gtggagtgat tggtgacatc
    42241 acagatgaag aattattgag atcagcaggg attgatacct gcgataccgt tgtagtcgcg
    42301 acaggtgaaa atctggagtc gagtgtgctt gcggttatgc actgtaagag tttgggggta
    42361 ccgactgtta ttgctaaggt caaaagtcag accgctaaga aagtgctaga aaagattgga
    42421 gctgactcgg ttatctcgcc agagtatgaa atggggcagt ctctagcaca gaccattctt
    42481 ttccataata gtgttgatgt ctttcagttg gataaaaatg tgtctatcgt ggagatgaaa
    42541 attcctcagt tttgggcagg tcaaagtctg agtaaattag acctccgtgg caaatacaat
    42601 ctgaatattt tgggtttccg agagcaggaa aattccccat tggatgttga atttggacca
    42661 gatgacctct tgaaagcaga tacctatatt ttggcagtca tcaacaacca gtatttggat
    42721 accctagtag cattgaattc gtaaagaggg atgacccctc ttttttgatg cccaagatgg
    42781 caaatagaga cagaagcccc ttgtcttcta gtaaaagttc ttcaaaggct ggactttatg
    42841 gtaaaataga aggaagtgac aagagagagt aatactcaat gaaaatcaaa ga
//
LOCUS       DAVDWT010000019        39898 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVDWT010000019 DAVDWT010000000
VERSION     DAVDWT010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39898
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.019"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            193..507
                     /locus_tag="VUW50_001144"
     CDS             193..507
                     /locus_tag="VUW50_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983049.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKRLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            517..723
                     /locus_tag="VUW50_001145"
     CDS             517..723
                     /locus_tag="VUW50_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7983050.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            723..920
                     /locus_tag="VUW50_001146"
     CDS             723..920
                     /locus_tag="VUW50_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983051.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            976..1656
                     /locus_tag="VUW50_001147"
     CDS             976..1656
                     /locus_tag="VUW50_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HET7983052.1"
                     /translation="MGMFVGMFKARVESHEIILDVKALMPWISAICLLIGFISMFLTF
                     NFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITGVAIPLAIFISLSEV
                     IILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFATPKDVLNYINSYDEG
                     ERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQLLAFLLVGAIHVYINVMQL
                     PMVKRYFK"
     gene            1668..2309
                     /locus_tag="VUW50_001148"
     CDS             1668..2309
                     /locus_tag="VUW50_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7983053.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            2327..2557
                     /locus_tag="VUW50_001149"
     CDS             2327..2557
                     /locus_tag="VUW50_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983054.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            2593..2673
                     /locus_tag="VUW50_001150"
                     /pseudo
     CDS             2593..2673
                     /locus_tag="VUW50_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            2909..4672
                     /gene="aspS"
                     /locus_tag="VUW50_001151"
     CDS             2909..4672
                     /gene="aspS"
                     /locus_tag="VUW50_001151"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET7983055.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITESLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVRGVDFKVFSEAPAVKAIVVTGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALVLEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            4650..5591
                     /locus_tag="VUW50_001152"
     CDS             4650..5591
                     /locus_tag="VUW50_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET7983056.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(5667..5747)
                     /locus_tag="VUW50_001153"
                     /pseudo
     CDS             complement(5667..5747)
                     /locus_tag="VUW50_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(5883..6869)
                     /locus_tag="VUW50_001154"
     CDS             complement(5883..6869)
                     /locus_tag="VUW50_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7983057.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(6879..7679)
                     /locus_tag="VUW50_001155"
     CDS             complement(6879..7679)
                     /locus_tag="VUW50_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HET7983058.1"
                     /translation="MLPYPFSYFSSIWGVRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTDKVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASFLLYITKRSRLFSFNTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(7932..8774)
                     /locus_tag="VUW50_001156"
     CDS             complement(7932..8774)
                     /locus_tag="VUW50_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7983059.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(8776..10068)
                     /locus_tag="VUW50_001157"
     CDS             complement(8776..10068)
                     /locus_tag="VUW50_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7983060.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(10179..11450)
                     /locus_tag="VUW50_001158"
     CDS             complement(10179..11450)
                     /locus_tag="VUW50_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7983061.1"
                     /translation="MSSKFMKSAAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            12000..13517
                     /gene="malQ"
                     /locus_tag="VUW50_001159"
     CDS             12000..13517
                     /gene="malQ"
                     /locus_tag="VUW50_001159"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET7983062.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            13543..15801
                     /locus_tag="VUW50_001160"
     CDS             13543..15801
                     /locus_tag="VUW50_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HET7983063.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAVVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(16846..17418)
                     /locus_tag="VUW50_001161"
     CDS             complement(16846..17418)
                     /locus_tag="VUW50_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983064.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGNWFVNDVALE
                     SAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(18041..18889)
                     /locus_tag="VUW50_001162"
     CDS             complement(18041..18889)
                     /locus_tag="VUW50_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7983065.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLVPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            19041..19319
                     /locus_tag="VUW50_001163"
     CDS             19041..19319
                     /locus_tag="VUW50_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983066.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            19321..21384
                     /locus_tag="VUW50_001164"
     CDS             19321..21384
                     /locus_tag="VUW50_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET7983067.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(21462..22718)
                     /locus_tag="VUW50_001165"
     CDS             complement(21462..22718)
                     /locus_tag="VUW50_001165"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET7983068.1"
                     /translation="MHIFDELKDRGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQELDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            22860..25325
                     /locus_tag="VUW50_001166"
     CDS             22860..25325
                     /locus_tag="VUW50_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET7983069.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            25390..26301
                     /locus_tag="VUW50_001167"
     CDS             25390..26301
                     /locus_tag="VUW50_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET7983070.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            26531..27229
                     /gene="dapD"
                     /locus_tag="VUW50_001168"
     CDS             26531..27229
                     /gene="dapD"
                     /locus_tag="VUW50_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HET7983071.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            27297..28427
                     /locus_tag="VUW50_001169"
     CDS             27297..28427
                     /locus_tag="VUW50_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HET7983072.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDDAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            28439..28978
                     /locus_tag="VUW50_001170"
     CDS             28439..28978
                     /locus_tag="VUW50_001170"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET7983073.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDLQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            28962..29639
                     /locus_tag="VUW50_001171"
     CDS             28962..29639
                     /locus_tag="VUW50_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET7983074.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(29716..30615)
                     /gene="galU"
                     /locus_tag="VUW50_001172"
     CDS             complement(29716..30615)
                     /gene="galU"
                     /locus_tag="VUW50_001172"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET7983075.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(30637..31653)
                     /locus_tag="VUW50_001173"
     CDS             complement(30637..31653)
                     /locus_tag="VUW50_001173"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET7983076.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            31865..32246
                     /locus_tag="VUW50_001174"
                     /pseudo
     CDS             31865..32246
                     /locus_tag="VUW50_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(32420..32845)
                     /locus_tag="VUW50_001175"
     CDS             complement(32420..32845)
                     /locus_tag="VUW50_001175"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7983077.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKSLTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(32913..33563)
                     /gene="phoU"
                     /locus_tag="VUW50_001176"
     CDS             complement(32913..33563)
                     /gene="phoU"
                     /locus_tag="VUW50_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET7983078.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(33578..34330)
                     /gene="pstB"
                     /locus_tag="VUW50_001177"
     CDS             complement(33578..34330)
                     /gene="pstB"
                     /locus_tag="VUW50_001177"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET7983079.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(34332..35147)
                     /gene="pstA"
                     /locus_tag="VUW50_001178"
     CDS             complement(34332..35147)
                     /gene="pstA"
                     /locus_tag="VUW50_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET7983080.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(35140..36003)
                     /gene="pstC"
                     /locus_tag="VUW50_001179"
     CDS             complement(35140..36003)
                     /gene="pstC"
                     /locus_tag="VUW50_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET7983081.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFIANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(36121..36996)
                     /locus_tag="VUW50_001180"
     CDS             complement(36121..36996)
                     /locus_tag="VUW50_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HET7983082.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(37163..38494)
                     /locus_tag="VUW50_001181"
     CDS             complement(37163..38494)
                     /locus_tag="VUW50_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HET7983083.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(38495..39202)
                     /locus_tag="VUW50_001182"
     CDS             complement(38495..39202)
                     /locus_tag="VUW50_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7983084.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(39286..39771)
                     /locus_tag="VUW50_001183"
     CDS             complement(39286..39771)
                     /locus_tag="VUW50_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7983085.1"
                     /translation="MAPTDKEDLIRTLELAPHIEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            39757..39898
                     /locus_tag="VUW50_001184"
                     /pseudo
     CDS             39757..39898
                     /locus_tag="VUW50_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT        11698 a         7483 c         8583 g        12134 t
ORIGIN      
        1 gaagctgacg tggtttgaag agattttaga agagtattaa tgatagataa agtggtcagg
       61 aacctactcc tgaccttttt cttttgcaaa atgacaaaaa tcataatttt tttgacaact
      121 atacttgtca aaaagaaaaa gatatgttac aatgaattca agttaagaaa taggaagcag
      181 aaaggagtta taatgtgggt actaggattt atactattta tgattttctt ttattctaat
      241 aattctaaaa aaatcaagaa actagagaat aaaatcaaaa gacttgagcg aaaagagaaa
      301 ggaaacgcag aaatgtcgag attattacaa gaaatgattg gaaaggaacc aattataacg
      361 ggagtgtata ttgggccaga taactgggaa gttgtggatg ttgatgagga atgggtaaag
      421 ctacgacgtg tagataatac gggaaaagaa aaattcaagt tgcaacgtat tgaggatatc
      481 caaaccgttg aatttgacgg agagtaggtg tgtaacatgc aactaaaaaa tcgtctaaaa
      541 gagcttcgag ctcgcgatgg tctcaatcaa accgacctag ccaaactggc aggggtttcc
      601 agacagacca ttagcctact agaacgggat gagtacaccc catccattat cattgccctg
      661 aaaatatctc aaattttcaa cgaaacagtc gaatcggtat ttcgtttgga ggaggacgag
      721 tgatgaacaa gtataaagtg atttattatg tagtggtcat agctttatta gtcagcgtat
      781 ttctactgat tgggatggac ctaagctggt ttaatcccta tcaaagcgac caatttgttt
      841 gggtctactt tgctctcatc ccagtaattg aatggattga aaagaaatcc aaaaatctag
      901 caagtgaaaa aggagaatga atatgaaaaa gaaacgtaga ttgttatttt tgatgtctat
      961 tgtcttgggg ggttcttggg catgtttgta gggatgttta aggcacgtgt cgaatcccac
     1021 gaaattattt tagatgtaaa agccttgatg ccatggatat cagctatttg tttactgata
     1081 ggtttcatta gtatgttttt gactttcaat ttcttaaaga aaagcagaaa atttcattcc
     1141 ttgtatcaag aggaaatgga tgacgatctg aatgaaacct attatgtgca aatgtatcgg
     1201 aatcttgagt ttggaaccat tgcttttaat attacaggtg tagcgattcc attggctatt
     1261 tttatttcat taagtgaggt gattatattg catacaaacc ctcaaacatt tttcctttct
     1321 ttcttactct ttgtggtatt cttagtcgct caaaaatctc tttttaaaac cattgcgatt
     1381 gttcgtcagt ttgatttgga atttttcgct acaccaaagg atgtcttgaa ctatataaat
     1441 tcttatgatg aaggggagcg tcaggctaat ttggaacaga gttttcgaat tttattccaa
     1501 ttacaccaat atgtcttacc agccttatat atttttctta ttatcatttc tttcttgaca
     1561 ggagagattc agttactagc tttcttgctt gtaggagcca tccatgttta tatcaatgtg
     1621 atgcagttac ctatggtaaa acgttatttc aaataaagga gttatctatg aaattactta
     1681 aaaaccttgg ctggattctt ctagcccttc tatccttttt atttatctat ggctttattc
     1741 aggggctcgc gactgcgtcg cttgctttag gcgcttcacc ctatgctgtt actctactct
     1801 atgtggcctt ggctggagta tatgtgtacg gcatttacaa atggtatcag aaggctcctg
     1861 ttcatattga gaagagcggc tttaatcgat ttatttggct tcctgtcttg gtttggttct
     1921 tatctctggt cgttcaattc ttcttgccag atgatccttc agtaaatcag caaatagcga
     1981 cagacttgac cttgtctcaa ccacttttct cattctttgc tgtggttatt tttgctcctt
     2041 tgacggaaga gattgttttt agagggatgt tagcacgcta tctctttcct aagcaggaca
     2101 atagtaaacg aaccctgatt tttcttctgg tatctagtct tctatttgcc ttgattcatt
     2161 ttccaggtga tgtgcaacaa ttttttgtct attttagcct tggttttagt ttgggtttgg
     2221 cttacattag cagaaaaggt ctggtctaca gtatttctct tcacgctttg aataatttag
     2281 tcggcttttt gatgattctc atgctataat agagtcagga ggtcacatga aacgagtaat
     2341 tttattagca gtgattcagg cagtcgttct atttttcatc attggagcgc tagcttatgc
     2401 cttcaaaggc gatttcttct ataactatct agcagttgtc tttgctccta ttgcaggtgt
     2461 actgcgtttt gggacggctt acataacgga aattgtcttg cctcgaaagg cagccgaaat
     2521 cgctgaaaag cgtaaagcag gcaaaaattc aaaataaaag agtccggtga agttcatcgg
     2581 atttttgtat tatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgcaggttgc
     2641 tcaaaacact gttttgaggt tgtggataga actgacgaag tcagctcaaa acatggtttt
     2701 gaggttgtag atgaaactga cgaagtcagt aaccatacat acggtaaggc gacgctgacg
     2761 tggtttgaag agattttcga agagtattag cgctaatttt tagggctttc acctgacttt
     2821 taaagactgg caaacttttc tgatgatttt catgaagagc ctgcgctttt atggtaaaat
     2881 agtaacagaa taaaagagga gagaaataat gaaacgtagt atgtatgctg gtcgtgttcg
     2941 tgaggaacac atcggacaag aaataacctt gaaaggatgg gttggccgtc gtcgtgacct
     3001 tggtggtttg atctttatcg atcttcgtga ccgtgaagga atcatgcagt tggttatcaa
     3061 ccctgaaaaa gtatctgcag aggttatggc aacagctgaa agccttcgta gcgaatttgt
     3121 tattgaggtg actggtcagg tcgctgcgcg tgagcaagcc aatgataagt tgccaactgg
     3181 tgcggttgag ttaaacgtga cagctcttat tgtgcttaac acagctaaga caacaccatt
     3241 tgagattaag gatggcattg aggcaaatga cgatacacgt ttgcgttacc gttaccttga
     3301 ccttcgtcgt ccagaaatgt tggaaaatct taaacttcgt gccaaggtga cccactctat
     3361 ccgcaactac ttggatgagt tggagtttat cgacgtggag acaccattcc tttctaagtc
     3421 aacgcctgaa ggggcgcgtg attatttagt gccgtctcgt gttaataagg ggcattttta
     3481 cgctcttcct caaagtccac aaatcacgaa acagctcttg atgaatgctg gttttgaccg
     3541 ttactaccaa atcgttaaat gtttccgtga cgaggacttg cgtggagacc gccagcctga
     3601 atttactcag gtcgacttgg aaacgtcctt ccttactgag caagaaatcc aagatattac
     3661 agaaagcttg atcgcgcgcg tgatgaagga aacaaaaggc atcgaagtaa cgctaccatt
     3721 ccctcgtatg aaatacgatg atgctatggc tctttacggt tctgacaagc cagatactcg
     3781 ttttgacatg ttgcttcagg acttgacaga agtggtccga ggtgtagact ttaaagtctt
     3841 ttcagaagca cctgctgtaa aagcgattgt ggtcacagga gctgcggaca actattcacg
     3901 taaagacatc gacaagatga cggaagtagc caaacagtat ggtgccaaag gtcttgcttg
     3961 ggtcaaggtg gttgatggag aattaaacgg accagttgcc aagttcttga ctggtatcca
     4021 agaagaattg acaacagcgc ttgttcttga agataaggac ttggttctct ttgtggcgga
     4081 tacgcttgaa gtggctaatg caacactggg tgcccttcgt ggacgtattg ctaaagagct
     4141 tggcttgatt gataatgata agttcaactt cctttgggtg gttgactggc cgatgtttga
     4201 atggtctgaa gaagaaggcc gctacatgag cgcccaccat cctttcacac ttccacagga
     4261 agagactgct catgaattag aaggtgattt ggctaaggtt cgtgccattg cttacgatat
     4321 cgtcttgaac ggttatgagc ttggtggtgg tagccttcgt atcaaccaaa aagaccttca
     4381 agaacgcatg ttcaaggctc ttggtttctc aactgaagaa gccaatgacc agtttggttt
     4441 ccttcttgaa gccatggact atggtttccc accacacggt ggtttggcta tcgggcttga
     4501 ccgttttgtc atgttgcttg ctggagaaga aaatatccgt gaagtcattg cctttcctaa
     4561 gaacaacaag gcaactgacc caatgacaca agctccatca acagtcgctc tcaaacaact
     4621 agaggaactc agcttacaag tagaagaaga tgaaacaaac aaaacgaatt aagcggtggc
     4681 gctattatct gcgccgcttt gcttatcaga taaaaatttt acgtgtctta caaagtatct
     4741 ctcgagaaaa gtatgatgag aagatttcgg cctctctggt ctatggtttt ttatcagcag
     4801 tagcagttaa tttctttttc caaccagggc atgtgtattc gagtggtgca acaggtctgg
     4861 cacagattat ctctgccttg agtaatcact ggtttggttt tcatattccg atttcgctaa
     4921 gcttctacgc cattaacttc cctttgatgg tcttagcttg gtatcagatt ggccataagt
     4981 tcaccgtctt tacctttatc acggtatcta tgagttcctt ctttatccag tttgtccctg
     5041 tggcaacctt gacagaggat cccattatca attccctttt tgggggtgtt gttatgggtt
     5101 tggggattgg ttttgctctt cgaaacaata tctccagtgg tgggacggat atcgtcagcc
     5161 tgactattcg taagaaaacg ggtaagaatg tcggtagtat ttctttcttg gtaaatggaa
     5221 ctatcatgct gatagcaggt ttgacctttg gttggaaata cgctctttat tctatgatta
     5281 ccatctttgt ctctagccgt gtgacagacg cagtctttac taagcaaaag cgtatgcagg
     5341 ccatgattgt gacaaatcat ccagagaagg taattgaaaa aatccataaa aaattgcacc
     5401 gcggagcaac catgatccac gatgcagaag gaacctataa tcacgagaga aaggcagttt
     5461 taatcactgt cattacacgt gcagagttta atgaatttaa acagattatg acacaagtgg
     5521 atccaagctc ctttgtctct gtctcggaaa atgttcatat tctaggaaga tttgttgaga
     5581 tagataatta gtgattaata ctcttcgaaa atctcttcaa accacgtcag cttcaccttg
     5641 ccgtaggtat ggttactgac ttcgtcagtt tcatctacaa cctcaaaaca gtgttttgag
     5701 caacctgcgg ctagcttcct agtttgcact ttgattttca ttgagtataa aaagatcttg
     5761 ggaaaagact caatttcagt agaaaaggaa gtaaatcttc ccacaataaa acgcatagta
     5821 tcaagttttt tcaatacctg atagtatgcg tttttgcttt taacgacttt ttgcccagtc
     5881 tcttattttt cgataatttt gtgggcgatc aattgacggt aacaaatttg tttattgttt
     5941 ttattatcat taataatctg gagaatagtt tcaagggaaa cacgaccaag ctctaaacta
     6001 ttgatatcga cataggctgc caagttgagc ttgggattaa ccgagtcaaa gctgagaaca
     6061 gggacatcca gctggtgttt ggcaatatag ttacaaacac cttcagctag gaggctatcg
     6121 gttgtgatga tagcatcaat ttgtggatcg tgcttgaata atcgcttgct aaatttatag
     6181 cccttttctt ccagaaactc gtcggcaaag tagatgcgat tgttgtcagt ggtaagtttg
     6241 taatgtttaa gcgcctgttc atagcctgtt aaacggtctt tggtcacgaa gagcttttta
     6301 cttcctccga taaaggcaat gcgtttgcag ccttttttga tgaaatattc agtcgcatca
     6361 aaaccagctt gaacattgtc gttgtcgaca agtgggatga aaggagatag agatttacct
     6421 aagataagga aggggaactg ttcttctgcg acgagtttta cgagagggtc ttcttcttgg
     6481 gcatagagaa aaattagccc atctacacgc ttgccgtaga ccatttgtga aatagcgttg
     6541 agacgctcct tctcatcttt ccctgttgct atctgaatgg catagtggtt ttcagatgcg
     6601 acttgagaga tgccacgtag aaccgatgga aagaaaggat tctggtagaa ggcgtctgag
     6661 tcatcaggaa gaactaatcc gataacctga gtatagctgc ttaccaagct acgagcgttg
     6721 aggtttgggt ggtagttgag ttccttcata gctttgcgaa cacgtttttt tgtttcgtcg
     6781 ctaatggttg atttattttg aataacacgg gttacggttg aaggcgaaac accagcagcc
     6841 ttggccacgt ctttaatcgt aacgggcata aaaatctcct atttatggta atttggatca
     6901 cggaaatgtg ttttataaaa gatagtgacc agatacagaa caaaaagaat attttgtaca
     6961 gtaatcaggg ttgtcatatt ttggccaaat aatcccaaaa taagtgtaat cagagtcggc
     7021 aatcctaaac agttcaagat aaaatggtag cactctttaa aggtattaaa tgaaaagagg
     7081 cgtgatcttt tggtgatata aaggagaaag ctagctccaa gagagacgat aaagaaattc
     7141 aaaccaaaga ggaagctcgc accgagaact aggaagaggc tgatatagac acgattttgt
     7201 tggtaccagt ctttagaaat tgcttgggtc aagctgtctt tgcttttgaa actctcagtc
     7261 tgaatggctc ggtaagagat gcgggtcagt tccttgcttt ccttgctgat gactagctca
     7321 tttgtatcga aatgcagttg caagtcctta ggtaattcct tgatttgact tggaccaatc
     7381 acaacagaag gggcttgact agctgttcca gtataagtta atgtgccatc gacaattgta
     7441 gcatgttcag agagatcctg gacaacctta tctgtcagag gttcatagac attatcgata
     7501 aaagtttcta gcggataggt ctcctgggag ctgttttgga tagcaatggg taccatagac
     7561 aagctgataa ggaagatact ggtaaagaga agttgaaacc agttgagctc gaaacgtttg
     7621 gacaggggct tacgaacccc ccaaatactt gaaaaatagg aaaatggata tggaagcata
     7681 ggtatctttc taaaaggtgt tttctatatg agtattatta tatagagaaa tgcgctttat
     7741 ttcaagtcta ggagaaaaat tgcttgctac aaggatatta tttacagtga caattgctaa
     7801 taatcaatga aaatcaaagt gcaaactagc cacaggttgc tcaaagcaca actttgaggt
     7861 tggagataaa gctgacgtgg tttgaagaga ttttcgaaga gtataaaatg aaaaagggtg
     7921 gcggggataa attatccctt gtcgccacca cttgtaagtc ctgaaacaaa gttcttttgt
     7981 aggaagaaga agagaataca gattggaagg gcgatgagga tagcacctgc tgagaagtag
     8041 gcaatcttca agtttttcgc attgttaacg aaggtttgga gacctacggc aacagtaaag
     8101 tattctttct cacgaagcaa gaaactagag aggatgtagt ccccgaaagg tcccatgaag
     8161 gcccagagag cttgtacggc aaccattggg cgaacaagtg gtagaacaat ttgccagaag
     8221 cggcggaagt gtcctgcacc gtctagtttt gcagattcgt ctaaagacat tggcactgta
     8281 tcgaagtagc ctttcatgag ccaagcattc atcgggatac caccaccaac gtagaggaag
     8341 atgaggaacc agttgtggtt aagggcgttc aacataagcg ccataacgaa gaaggctgtc
     8401 aaagcggcca tagttggcac catttggatg atcaagaaga agaccaaact ttgtttacga
     8461 gccaagaagt tgtaacggct gtaagcataa ccagcaagta cgatgatact tgtttgaaca
     8521 gccatggtaa ttaaggcgat aatcaaagtg ttgaggtacc aagtaccgta caaggtttca
     8581 gtgaagaggc ctttaaagtt atcaaaattg aggtcgatat tagtatctag tttaaaggct
     8641 gagacgttac ctgctttaaa ggctgacata atggtaatca acagtggata gataattaca
     8701 attgatagac caatcaggta aaggtaagta aggctttgag tcagtctacg tttgagttta
     8761 attgagttat tcatcttaga cgtcctccat atcaaatgcg tgtagtttct tgaatgcgat
     8821 catagagatt gagatgacaa tgatagagat aatcaaggta acagctgccg ccattgagta
     8881 ttgaggagat gtacctgttg tcaaacggta gatccatgag atcaagatat cggttgaacc
     8941 agctccacct ccgacactac caggtcctcc accattgaag aggtacatga tagagaagtt
     9001 gttaaagttg aaggtgtatt ggctaatcaa agtaggtgcc gcaacagcca aaatcattgg
     9061 gaaagtgatg ttgcggaatt tttgccaagc gttggcaccg tcaatataag ctgcttcgta
     9121 aaggtcgtta ggaatagatt gcaagatacc caaggtcaga acgtagatgt atgggaatcc
     9181 gagccaacct tgcatcataa tcaaggcaat cttagtccaa gttgggtctg ttttccaagg
     9241 aataagagct ccatcaagga aaggaaggaa tttagccaag attggcaata cttgagtgtt
     9301 gatagcaccg acactatcgt taaacatgtt tgagaatgtc aagatagtga tgaaggctgg
     9361 gacagcccaa ggaagaagga aaataacacc aaagatacgt tttcctttga taaatggttg
     9421 gttggcaatg atagctgtga agataccaat tacgatttgt aaagtagaag ctgccaaagc
     9481 ccaaatgata gtccaagaaa gaacagaacc aaaggcagaa cggaaggtac tcaagctcca
     9541 aatgtttgta aagttggtca aaccaaccca gtccaacaac ttgtttggtg gcaagtgttg
     9601 gaagtcgtag ttggtaaagg cgatcatcaa ggttacgata actgggaaga taatcgcgaa
     9661 ggtcatggca acataagatg gaatgatcaa gaggtaaggg aagccatttt cataaatccc
     9721 tttgatcatg tctttgagtg tgcgtggaac tggaattcca ttgttaatgc gttttgcaat
     9781 cgtatgtgca tctttgatat ttgagaaata aaagagtaca taaacgatta caaagattag
     9841 atggaaggca ccacgaatca gcataaagag ggaattatca cgacctggtt tgtcaccaag
     9901 agtgatgagg ttgctcaatt caggggttgc aagtgctagg aagtaaagga caaatacgat
     9961 ggttacacca aggaagataa aacctttggc tttttgttta ttgtaaatct gtcctaaccc
    10021 aggaatgata gacagcaggg ctgctttact aggttgttgc ttttccatat taactccttt
    10081 cataggatac tactttttta taagaaagct aaattatttg tgtctctatt gataaattca
    10141 aagggggatt tgatttccac cccccttgaa caaatttttt attcaccaaa tttttgtttg
    10201 attgtttctt tgatcaatgt tacagcatcg ttagcagctg ttttagcatc tttttgacca
    10261 cttacagcat caaagagcat atttttcgct ggatcccaaa ctgcagacat ttgagagatg
    10321 tttggcagtg gttgagtgtt cttgaactgt ttgataacag ctgttgtcaa ctcatcgttt
    10381 ttaccttcag cgtatgaacg agcctcagta ttagctggga tttcgttagt cttatcatat
    10441 aatacttttt gttgttcagt tgcaacaagg aagtctacaa atttttgaga agcttcaagg
    10501 ttcttaacgg cttgaggaat gacccaagct ttaccaccac cgaatgcagc atattctttt
    10561 ccatttggaa gagttgggat agttgcaact ccgtagttta ctttagcatc tttaaaggct
    10621 tgagctttcc aaggtccgtc gatgatagca gctgttttac cttcttggaa ttgagtttgg
    10681 attaagtttc cagcaccttc tgtatcttgc atacctttag gccatttttc gtaccaagat
    10741 ttagcgtagt tgatacctac gatagaaccg tcgtttgcaa gaccgatgtc tttagcgtct
    10801 ttaccgtttt ggccaaagac gtaagcaccg ttaccggcaa gaagtccata tgtatagtag
    10861 aagtttgtcc agtcagctag gaaggcagta gttttaccat cttcaccagc gaatgcgtat
    10921 ttgctatctt tagcaaggtt ttccaagtca gcaaatgttt ttggagcatc tttcaccaag
    10981 tctttgttgt agtacataac aagtgactcg ataacggcag gagcaccgta aactttacca
    11041 ttagcagctg ttacaagaga tttagttgtg tcgtctgttt tagcaccatc gctcaatttc
    11101 acttctgaaa gttgtccgtc agaaccaagg ctacctacac ggtcgtatgg agccatcata
    11161 acatcaggga cattaccaga ttggttgtca agagaaagtt tatcaagacc tcctagagca
    11221 tcaccagttt taagagtgac ttttactcca gcttcttttt cataagcttt agcaacctct
    11281 tcaatatagc tcttatatcc ctcgtctaca tatacagtga gttctttgac ttcagatgaa
    11341 ccagaatcag caggcttatc agcagttttg cttccgcaag ctaccaaaag caagctagca
    11401 agtgtagcag ttccaagcac cgcagcgctc ttcataaatt tagatgacat agtgtattcc
    11461 tcctaaagaa tagcaagttt tattgataag gaaacgcaaa cgttttcctt tatgagctta
    11521 gtatagcaca aatagaaaac ggttgcaagt gttttttgta aaaattttaa aaaattttta
    11581 agcttgattt gatagcataa ttttgcattt taatagaaga aaagtatgaa atagataaga
    11641 atacagtatt tttaataaaa atatatgaaa tctgtctaaa gagggaaatc aggaggggct
    11701 accttcctat ttctaatcta tatctatttt ctatagacgt ttgtgctttg ttgcatataa
    11761 attcaagctc cctaaggagg ttttgtactt tgtatactgt ctcgtgagag tcaaacttta
    11821 ggattagata gttttaagtt atctttgcta aaatgagtta gtctccttaa aaataatcaa
    11881 aaaagttttt taaaaacgct tgcaattatg cttgaaaagg agtatactta taagtaacgc
    11941 aaacgtttgc gtctgaaaaa tacgcaacgt tccattattt taacacacga ggtgctatta
    12001 tgaaaaaacg tcaaagtggt gtgttgatgc acatctcttc tcttccagga gcttacggaa
    12061 tcggatcatt tggtcaaagt gcttacgact tcgttgattt cttggtccgt acaaaacaac
    12121 gttactggca aatccttcca ttaggagcaa ctagttacgg ggattctcct taccaatctt
    12181 tctcagcctt cgcaggaaac actcatttta tcgatttaga tatcttggtg gagcaaggtt
    12241 tgttggaagc aagtgacctt gaaggagttg actttggtag cgatgcgtct gaagttgact
    12301 atgctaaaat ctactatgca cgtcgtcctc ttttagaaaa agcggtgaaa cgtttctttg
    12361 aagtcggaga tgttaaagat tttgagaaat ttgctcaaga caaccaatca tggcttgagc
    12421 tctttgctga gtatatggct atcaaagagt attttgacaa tcttgcttgg actgaatggc
    12481 cagatgcaga tgctcgtgct cgtaaagctt cagcacttga aagctatcgt gagcaattgg
    12541 cagacaagtt ggtttaccac cgtgtgactc aatacttctt cttccaacaa tggttgaaat
    12601 tgaaagctta cgctaacgac aaccacatcg aaatcgttgg ggacatgcca atctacgtag
    12661 cggaagattc aagtgatatg tgggcaaatc cacatctctt caaaacagat gtcaatggta
    12721 aggctacttg tatcgcagga tgcccaccag atgagttttc tgtaactggt cagctttggg
    12781 gtaatccaat ctatgactgg gaagcaatgg acaaagacgg ctacaaatgg tggattgaac
    12841 gcttgcgtga aagcttcaaa atctacgata tcgttcgtat cgaccacttc cgtggcttcg
    12901 aatcttactg ggaaatccct gctggttccg atacagcagc acctggtgag tgggtgaaag
    12961 gtccaggtta caagcttttt gcagccgtta aggaagaact tggtgagcta aacatcatcg
    13021 cagaagacct tggcttcatg acagatgaag tgatcgaatt gcgtgaacgt actggcttcc
    13081 caggaatgaa gattcttcaa tttgccttca acccagaaga cgaaagcatt gatagcccac
    13141 acttggcacc tgctaactca gttatgtaca caggaacaca cgataacaat acggttcttg
    13201 gttggtaccg taatgagatt gatgatgcga ctcgtgagta catggctcgt tacacgaacc
    13261 gtaaagaata cgaaacagtg gtacacgcta tgcttcgtac agtattttca tcagttagct
    13321 ttatggcaat tgcaactatg caagatttac tagaattgga tgaggcagct cgtatgaact
    13381 tcccatctac ccttggtgga aactggtctt ggcgtatgac tgaagatcaa ttgacaccag
    13441 ctgtcgagga aggtttgctt gacttgacaa caatttatcg ccgaattaat gaaaatttgg
    13501 tagatttaaa gaaataagac aataatcagg agacaactaa acatgttatc actacaagaa
    13561 tttgtacaaa atcgttacaa taaaaccatt gcagaatgta gcaatgaaga gctttacctt
    13621 gctcttctta actacagcaa gcttgcaagc agccaaaaac cagtcaacac tggtaagaaa
    13681 aaagtttact acatctcagc tgagttcttg attggtaaac tcttgtcaaa caacttgatt
    13741 aaccttggtc tttacgacga tgttaaaaaa gaacttgcag ctgcaggtaa agacttgatc
    13801 gaagttgaag aagttgaatt ggaaccatct cttggtaatg gtggtttggg acgtttggct
    13861 gcctgcttta tcgactcaat tgctactctt ggtttgaatg gtgacggtgt tggtcttaac
    13921 taccactttg gtcttttcca acaagttctt aaaaacaacc aacaagaaac aattccaaat
    13981 gcatggttga cagagcaaaa ctggttggtt cgctcaagcc gtagctacca agtaccattt
    14041 gcagacttta ctttgacatc aactctttac gatattgatg ttactggtta tgaaacagcg
    14101 actaaaaacc gcttgcgttt gtttgacttg gattcagttg attcttctat tattaaagat
    14161 ggtatcaact ttgacaagac agatatcgct cgcaacttaa ctctcttcct ttacccagat
    14221 gatagtgacc gtcaaggtga attgctccgt atcttccaac aatacttcat ggtttcaaac
    14281 ggtgcgcaat tgatcatcga cgaagcaatc gaaaaaggaa gcaacttgca tgaccttgct
    14341 gactacgcag ttgtccaaat caacgatact cacccatcaa tggtgattcc tgaattgatt
    14401 cgtcttttga ctgcacgtgg tatcgatctt gacgaagcaa tctcaattgt tcgtagcatg
    14461 actgcctaca ctaaccacac aatccttgct gaagcgcttg aaaaatggcc tcttgaattc
    14521 ttgcaagaag tggttcctca cttggtacca atcatcgaag aattggaccg tcgtgtgaag
    14581 gcagagtaca aagatccagc tgttcaaatc atcgatgaga gcggacgtgt tcacatggct
    14641 cacatggata tccactacgg atacagtgtt aacggggttg cagcactcca tactgaaatc
    14701 ttgaaaaatt ctgagttgaa agccttctac gacctttacc cagaaaagtt caacaacaaa
    14761 acaaacggta tcactttccg tcgttggctt atgcatgcta acccaagatt gtctcactac
    14821 ttggatgaga ttcttggaga tggttggcac catgaagcag atgagcttga aaaacttttg
    14881 tcttatgaag acaaagcagt tgtcaaagaa aaattggaaa gcatcaaggc tcacaacaaa
    14941 cgtaaattgg ctcgtcactt gaaagaacac caaggtgtgg aaatcaatcc aaattctatc
    15001 tttgatatcc aaatcaaacg tcttcacgag tacaaacgcc aacaaatgaa cgctttgtac
    15061 gtgatccaca aataccttga catcaaagct ggtaacatcc ctgctcgtcc aatcacaatc
    15121 ttctttggtg gtaaagcagc tccagcctac acaatcgctc aagacattat ccatttaatc
    15181 ctttgcatgt cagaagttat tgctaacgat ccagcagtag ctccacactt gcaagtagtt
    15241 atggttgaaa actacaacgt tactgcagca agtttcctta tcccagcatg tgatatctca
    15301 gaacaaatct cacttgcttc taaagaagct tcaggtactg gtaacatgaa attcatgttg
    15361 aacggagctt tgacacttgg tactatggac ggtgctaacg tggaaatcgc tgagttggtt
    15421 ggagaagaaa acatctacat cttcggtgaa gattcagaaa ctgttatcga cctttacgca
    15481 aaagcagctt acaaatcaag cgaattctac gctcgtgaag ctatcaaacc attggttgac
    15541 ttcatcgtta gtgatgcagt tcttgcagct ggaaacaaag agcgcttgga acgtctttac
    15601 aatgaattga tcaacaaaga ctggttcatg actcttcttg atttggaaga ctacatcaaa
    15661 gtcaaagagc aaatgcttgc tgactacgaa gaccgtgacg catggttgga taaagtcatc
    15721 gttaacattt ctaaagcagg attcttctca tctgaccgta caatcgctca gtataatgaa
    15781 gacatctggc acttgaacta atactcttcg aaaatctctt caaaccacgt cagctttatc
    15841 tgcaacctca aagcggtgct ttgagcaacc tgcggctagc ttcctagttt gctctttgat
    15901 tttcattgag tataagatac aaatttatac taatacatct tgtaaaaaag cgagtttcga
    15961 ttgaaattcg cttttttaaa tgctgtggat ttgggtcaat cttgtctaaa aatagggaaa
    16021 tcctagatac agtgaaggct ttaaatgctg gtttttgctg tcttcagcct tatatttttt
    16081 cgtagttggt tacctcatat ctcttgtatt cgcttacata aagtattata atataattgt
    16141 aggaaagaag gtgtttttat gatatacaca cttaaattgg tgttgtttat tacctttctt
    16201 gtaataagct tgttacctga taagattttt ggaaaaaata aaaaaatttg gaaaatagtt
    16261 tttgcaatat tgacggcagt ggcagcattg tcatttatgt actaagttat tttaagaatg
    16321 tagggaaata aaccctacat tctttttagt tttttctgtt ttctaaattc tatttatcca
    16381 agcgcttcaa catttcttgc ttcttcgctt caagttctgc acgcttttct tcgatttcgg
    16441 catgtttttt ctcgagttca gaacaacttg caccattgct aaattctttt cgccatcagg
    16501 agatagggtg agtcgacatg tctattactc acccaaagca gtcctacaaa gcaggaattt
    16561 tctgttactt ttttggaaat agtaacgttt atacagcttt gacacttcgt atcaaagcgc
    16621 caaacacact ccgagggatt tacagaaagc agaaaaggaa tgatctggta taagatcatt
    16681 ccttttctct ctttttcttt aagtaattat atacaatgta cgacgaagtc gtcattgcaa
    16741 tgctgatcca ccacctaaag ggaactttaa acaacattga taagataaag aatataaaca
    16801 acgaaaatac gttataccca attaatttta ttgtatatct catgattaaa agttaatcct
    16861 tccgttgtta ggaatggcat catttttatc ccataattgt gctaaataag tccccggtga
    16921 taataaattc atagcggatt ctaaagcaac atcatttaca aaccaattac ctagatatct
    16981 agaaattgct gaacgaatag cactttttgc tgcatgtttt ccttttactt taattagatt
    17041 tgcaaggcct gcagtagttc ctcctaatgc taaagctatt gcagtatcta atagagcacc
    17101 catttgatta actgtaatac cttgccaagc tgctctaaat ggagagtatg taggtgggat
    17161 tgtataatcg ccttgtaatt gtcggttaat tacttctttg atccattgtt gtgagacgtc
    17221 tggatgaaaa gattggattt cgtttgcaag tgtattgatt tgttcttctg ttagagaagt
    17281 gacaggttga agttccatat ttgtttcaat ttgtgatact tgttcagaag cgtatacagc
    17341 tgaaacactt ggaatcgctg atacaattaa cacaattgac gtcaaaaaaa ccgaaataaa
    17401 tttcattaat ttgttcatga gcttttctcc tttttatttg tatctgctta cattttatta
    17461 tatactgtta ttatagtcaa gaaaaatatg ctattatgtt aaaaaaatat ttttaaaaat
    17521 ataaatggac ggatttattt tggattttat ttgttatttt gacctgcctc tatattggta
    17581 accatgattt gtttactttc aatcatcaag aattctcttt tcgtggtagc gtttggggtc
    17641 tggtactggc cttatatcac ttattgttca ttgataagtt tgtaatttcg aatcgaaaat
    17701 aaagattaga actatgattg actggctact tttaatattg attttggagg aagattttgt
    17761 ctctattatt tattatttta aatttattta ttttgtataa gatctattct ttgaggcggg
    17821 aaaaatcgaa atacttgatt tatacggcct atatcatatt tggggtaaat gtagtatatg
    17881 gtattcaatg gttattaaga gaactgattt caactatttc cccttaatgt gtatggaaac
    17941 tataacaaaa cgcataagat taaggtttgt gtacctgttc ttatgcgttt ttataatatt
    18001 gaagattttc ggtaagtttt atacctttgg gaagtctagt ttagaatgct ttcccaacca
    18061 aaatctctgc ttcgatggta atctctgtca gttggctcca gtcaatcttg ctctggtcaa
    18121 cgtgaaagag gagaggagtc atactgagta gggcttggag ttgttctgct gtgatagtct
    18181 tagtcagaga ggcagtttga ctagatagga tggtaaagtg ttcctggaaa tgctccttga
    18241 tatcttggtt agaatactcc ttgtttgtca gctggtcctg tactctttga cggatttctt
    18301 tgaggtgatt ttcagttggg ataaccttta tcaagatacc gtctttggat aaaacgcgac
    18361 gaaattctcc atagttggca ggtgagaaga tatcaagcag aatatccatg ttagcgtctt
    18421 ttataggaag tcgtgccaag tcgccaacga accaattgac tgcccagttg ggttcacttt
    18481 tagcagcgat ttggactgaa tctttggaga tgtcaaaggc atagaaagtt ttttcagagt
    18541 gactttcttg tagtttgcga gaatagaatc cttcaccaca accgatatcc aaaattgttg
    18601 tggtagtttt tgagcttgca agcaagtcag atacagcatc taagatagct tggtaaaagc
    18661 cggcttctag gatttgttga cggttttgaa aattttcctt gtcgtagtta gcagattgct
    18721 tgatttgagg gactagattg acatagccaa atttcgccaa gtcaaaagaa tgacggttgc
    18781 agcacttgaa attagtctct aacagagtca gattttcttg acagatagga caggcaaagg
    18841 cagtcgcaga agcaaaacgc tgaagtttgg gcttgagatt tgtattcata gtttaagtgt
    18901 atcaaaagag gcaaatgaaa gcaactttag gaataagtag atgggagatt cagtagaaat
    18961 aaaactaatt ctccaattta tagaatgaaa ttgcttttat atctaaattg ctatataata
    19021 atgataagga ggaaataagt atgttaaaag aagtattaac cgttgcaaaa gttgcgaaaa
    19081 aatcttcatt atttttgggt ggtgtcgcat ttggtaccct tggtttgaaa atcttagcaa
    19141 gtaaggaagc taaaaaaggt tattctaaag ctttggctaa ggcttacaag ttgaaagacg
    19201 agctagatgc atctgtttct gttgtgaagc aacatggaga cgatgtcttg caagatgcca
    19261 aatatttgta cgagcaagag aaaaaagaag agcaattaga tagccttata ggtgaataat
    19321 atgtctttta aagtgctaca tagaggatac caacatatcc gactatcatc ttctttttca
    19381 cttaccttgg atattcaaga ctatcttcgt tccttggcga gagatgaaaa ggggattgag
    19441 tctatccagt tttacatgga tcaacagcac tttactctac gcataaaaga aggcttttct
    19501 gtattagata atgcagaagc ctttttaaaa agaattgata aagggaaagt ttctgagttg
    19561 atgactcttc ccattcgtag agaagagagt gcttattcta ttgtttcagg tgcagcggtt
    19621 aagcgtgtac tttttcgtag ttttgtgccg tatcctattc gctatatatg gacttgttat
    19681 caggctttgg gttatattag agaagcctat caaacactag cgcgtaagga actaacgatg
    19741 gaagtcttgg actgttcggc tattttattg tctttgttta tgaaccaatc caagacagct
    19801 agcaatatca tgtttatgct tgatttgggg aatcatttag atcagtggtc cttgaaaaaa
    19861 actgcaacag atttagaaca gagtcttctt gcaaaagaga gcgatgtatt cctagtacag
    19921 ggcgatacgg ttgttagtat caagagttcc gatgttcaaa taggagatgt cttgatctta
    19981 tctcaaggaa atgaaattct gtttgatgga caagtagttt caggtttagg tatggtcaac
    20041 gaaagttcct tgacaggaga gagttttcca gttgaaaaaa gagagtctga tttggtttgt
    20101 gcaaatacag tattagaaac tggagagtta cgcattcgtg taacagataa tcagatgaac
    20161 agccgtattt tacagctgat tgagttgatg aagaaatctg aagaaaacaa gaaaacgaaa
    20221 caacgctatt tcatcaagat ggcggataaa gtcgtcaaat ataatttctt gggggctggg
    20281 ctgacttacc tattgacagg ttctttttct aaggctattt ctttcctatt ggtcgatttc
    20341 tcctgcgctt tgaaaatctc tactcctgta gcttatttga cagttatcaa ggaagggttg
    20401 aaccgtgaaa tggtgattaa ggatggagat gttctggaga aatatctgga agttgatact
    20461 ttcttgtttg ataagacagg aacaatcaca actagttatc ctatagttga aaaggtgtta
    20521 ccttttggag actatagtga ggaagatatt ctcagaatca gtgcctgtct tgaggaacac
    20581 atttatcatc ctattgctaa tgccatcgtc aagcaagctg agatagaggg aattgaacat
    20641 gaggaaatgc atgggaaact ccaatatatc gcaagcaagg ggatcaaatc tcatatagat
    20701 ggccaaccag ttcttattgg gaattatgtc ttgatgcagg atgagcagat tcatatcagt
    20761 tcagaacaaa atgctttaat tgaagagtac aagagtcact acaatctctt attcttggct
    20821 tatcaaaatg aattgattgg aatgttctgc attcatactc ctttgagaaa agaagcaaaa
    20881 acagccttgg ataaacttaa ggcacagggg aaaaaattga ttctggcaac aggagacacc
    20941 ttgattagga cagaggaatt agtcaaagat ttgccctttg atcaggtcta tacagacttg
    21001 aaacctgatg ggaaatttga gttagtagag aaactgcaga aagcaggtca cactattttg
    21061 atggttggag atggattgaa tgactcagcg gctttaactc tatcagatat cggtgtggtg
    21121 atgaatgaga gtgcagatat ttctaagcag atgagtgata tcttattgtt agataatcgt
    21181 ttggatttct tccaagagtt ggattcgtta tcatcatctt tgcaaacact catcaagaaa
    21241 aatattcagg ataccgttgt cgtaaatagt agtttgattg gctttggctt atttaattgg
    21301 ttcagtcctt caaatctctc tatcttacat aatctaacaa ccttgcgcat tgtcctgcgt
    21361 agcctgtcta ttaaaaatag atagatgaga gttattaaca gcaaaaagga gttacctttc
    21421 tcgaggttaa ctcctttgtt tatagataaa tgttgaatag tttagtaagt caatacaaag
    21481 tattttttct tcccacgacg gataacagtc agttcattct ctaacttatc agcgtcactc
    21541 aagacatagt caagctcttg gatgcggtcg ccgtttacgt agatggctcc gttttggacg
    21601 tcttcacggg cttggcgttt tgagttaact ataccagatg agacgagcag ttccacgata
    21661 ttgttgtttt cgtctgcctg tacttggtag tttggcacac cacgaagtcc ttgtttgagc
    21721 tctttgacag aaaggttttt gatgtttcct gcaaagagtt gctcagtgat gttaagtgct
    21781 tctttgtagg cttcttctcc gtgaacaagt gtaacaactt cacgagccaa gactttttga
    21841 gccaagcgtt cgtgtggcgc tgcttcaaat tgtttacgaa tatcttcaat ctcatcaagt
    21901 gacaagaaag taaagatttt caagaagcga acagcgtcag cgtccatcac gttcatccag
    21961 aattggtaca tttcgtatgg agaagtcttt tcgggattga gccagacggc atttccttct
    22021 gatttaccaa atttcttacc agttgcatct gtgattagtg gaacagtgat aacgtgacca
    22081 gtcttgtccg ctttacgacg aagcaattcg gtaccagctg tcatatttcc ccactggtca
    22141 gaaccaccga tttgaagagt gacattatgg tcttggttaa ggacgaagaa gtcgtaccct
    22201 tgcatgattt ggtaagcgaa ctcagtgtaa gaaattcctg tttcgattcg tttttttaca
    22261 gattccttac tcatcatgta gttgaccgtg aagtattttc caatatcacg gaggaagtca
    22321 atgaagctga tgctgccaaa ccagtcgtag ttgttgacca tgacagcctt gttttcgcca
    22381 ttttcaaagt caagaaaacg agaaagttgt ccttggatag acttgaccca gccatctact
    22441 gtgtcttttg tttggagact acgttcagca tctttgaagg acggatctcc gatgagacct
    22501 gtagcaccgc caacgagcgc ataaggtttg tgacctgcta gttgcaagcg acgacttgtc
    22561 aagattgcga caaggtggcc taggtgaagg ctgtcagcag ttggatcgta gccagtataa
    22621 taagaaactt gaccttcttc tagggcttta cgcaaagctt cttcatcagt cgtttgaaat
    22681 atcaaaccac gatcttttag ctcatcaaaa atgtgcatgt gtcttttctc ctttttaaaa
    22741 ttattgtttc tacctattgt atcacaaact tgggcaatag cctagcagaa tagggaagaa
    22801 agtggctatt ttattgaaaa tttccctttt gtggtataat agatagagtg aggaaatcca
    22861 tgcaaaatca attaaatgaa ttaaaacgaa aaatgctgga atttttccag caaaaacaaa
    22921 aaaataaaaa atcagctaga cctggcaaga aaggttcaag taccaaaaaa tctaaaacct
    22981 tagataagtc agccattttc ccagctattt tactgagtat aaaagcctta tttaacttac
    23041 tctttgtact cggttttcta ggaggaatgt tgggagctgg gattgctttg gggtacggag
    23101 tggccttatt tgacaaggtt cgggtgcctc agacagaaga attggtgaat caggtcaagg
    23161 acatctcttc tatttcagag attacctatt cggacgggac ggtgattgct tccatagaga
    23221 gtgatttgtt gcgcacttct atctcatctg agcaaatttc ggaaaatctg aagaaggcta
    23281 tcattgcgac agaagatgaa cactttaaag aacataaggg tgtagtaccc aaggcggtga
    23341 ttcgtgcgac cttggggaaa tttgtaggtt tgggttcctc tagtgggggt tcaaccttga
    23401 cccagcaact aattaaacag caggtggttg gggatgcgcc gaccttggct cgtaaggcgg
    23461 cagagattgt ggatgctctt gccttggaac gcgccatgaa taaagatgag attttaacga
    23521 cctatctcaa tgtggctccc tttggccgaa ataataaggg acagaatatt gcaggggctc
    23581 ggcaagcagc tgagggaatt ttcggtgtag atgccagtca gttgactgtt cctcaagcag
    23641 catttttagc aggacttcca cagagtccca ttacttactc tccttatgaa aatactgggg
    23701 agttgaagag tgatgaagac ctagaaattg gcttaagacg ggctaaggca gttctttaca
    23761 gtatgtatcg tacaggtgca ttaagcaaag acgagtattc tcagtacaag gattatgacc
    23821 ttaaacagga ctttttacca tcgggcacgg ttacaggaat ttcacgagac tatttatact
    23881 ttacaacttt ggcagaagct caagaacgta tgtatgacta tctagctcag agagacaatg
    23941 tctccgctaa ggagttgaaa aatgaggcaa ctcagaagtt ttatcgagat ttggcagcca
    24001 aggaaattga aaatggtggt tataagatta ctactaccat agatcagaaa attcattctg
    24061 ccatgcaaag tgcggttgct gattatggct atcttttaga cgatggaaca ggtcgtgtag
    24121 aagtagggaa tgtcttgatg gacaaccaaa caggtgctat tctaggcttt gtaggtggtc
    24181 gtaattatca agaaaatcaa aataatcatg cctttgatac caaacgttcg ccagcttcta
    24241 ctaccaagcc cttgctggcc tacggtattg ctattgacca gggcttgatg ggaagtgaaa
    24301 cgattctatc taactatcca acaaactttg ctaatggcaa tccgattatg tatgctaata
    24361 gcaagggaac aggaatgatg accttgggag aagctctgaa ctattcatgg aatatccctg
    24421 cttactggac ctatcgtatg ctccgtgaaa agggtgttga tgtcaagggt tatatggaaa
    24481 agatgggtta cgagattcct gagtacggta ttgagagctt gccaatgggt ggtggtattg
    24541 aagtcacagt tgcccagcat accaatggct atcagacctt agctaataat ggagtttatc
    24601 atcagaagca tgtgatttca aagattgaag cagcagatgg tagagtggtg tatgagtatc
    24661 aggataaacc ggttcaagtc tattcaaaag ctactgcgac gattatgcag ggattgctac
    24721 gagaagttct atcctctcgt gtgacaacaa ccttcaagtc taacctgact tctttaaatc
    24781 ctactctggc taatgcagat tggattggga agactggtac aaccaaccaa gacgaaaata
    24841 tgtggctcat gctttcgaca cctagattaa ccctaggtgg ctggattggg catgatgata
    24901 atcattcatt gtcacgtaga gcaggttatt ctaataactc taattacatg gctcatctgg
    24961 taaatgcgat tcagcaagct tccccaagca tttgggggaa cgagcgcttt gctttagatc
    25021 ctagtgtagt gaaatcggaa gtcttgaaat caacaggtca aaaaccaggg aaggtttctg
    25081 ttgaaggaaa agaggtagaa gtcacaggtt cgactgttac cagctattgg gctaataagt
    25141 caggagcgcc agcgacaagt tatcgctttg ctattggcgg aagtgatgcg gattatcaga
    25201 atgcttggtc tagtattgtg gggagtctac caactccatc cagctccagc agttcaagta
    25261 gtagttctag cgatagcagt aactcaagta ctacacgacc ttcttcttca agggcgagac
    25321 gataattctc taaatgaagt ggccaatcaa tggattgcca cttttttctt ttcgtgttac
    25381 aataagggta tgaatcagta tcagaaaaag attgttaacg gaaaaattta ttcgctccta
    25441 tccggcttaa tatggggaat ctgtggaatt ttaggagagt acttctttac tcattatcag
    25501 gtgtcttcgg gctggattac ctctatgcgt ttgacactgg cagggagtct tgtactcatt
    25561 tggtctgcaa tacaattaaa atcgcaagtg ctagatattt ggcgagacaa gaaaaattac
    25621 ctgccctttt tagcctatgc tattttgggg attttttcag ttcagtattt tttctatctc
    25681 tgtgtagaat actcaaatgc tacgacagca actattttac agtttattag ccctgtcttt
    25741 atcctctttt acaatcgctt ggtttatcaa aaacgagcgt caaaaagcgc tgttttctat
    25801 gttttggttg ccatgctggg tgtttgcttg atggcgacaa agggagacct ctctcagtta
    25861 tccatgacgc cgctagctct tataacaggt ttgctgagtg ccatgggtgt tatgtttaat
    25921 gttatcttgc cccaaccttt tgctaagcgt tatggttttg ttcctacggt tgggtggggg
    25981 atgattttgg caggtttgtt tagcaatgtc ctctcgccgg tttatcagct ttcctttact
    26041 cttgatattt ggagtatctt gatttgcctc attatcgctt tctttggaac ggcttttgct
    26101 tttttcattt ccatgaaggc tgtgtccttg gtttctcctt tggtggttgc cgttatcagt
    26161 gccagtgaac ctctctcttc tgctctcttg agtgttttgt tcttaggatt agtagtggat
    26221 tggtccctcc ttctagctat agccttgatt attttaccca tgatattttt gtctatagaa
    26281 gaagcgaaag aaagtagata aaaagtcttt tcttaattct aaaagtgtgc tatactagaa
    26341 tagtcaataa aacacgggga gatagttgtg aagagtgttt ttaggttgta tcggtagggg
    26401 cttgctttta ccgacagcac gttttatctc acatccctaa aaaatcatac ctaaaaacag
    26461 acaaaaaggt ttcaaatttg aggccttttt tggtagaata ggtatcatta taacgaacca
    26521 ggaggcacct atgactgcta caaaaatgaa cgctcaagaa attatccaat ttatcgccaa
    26581 tgctgaaaag aaaaccagtg tcaaagtaac ctttgagggg caactcgcaa ctgctgtgcc
    26641 tagctctgtt gtcaaactag gaaatgtcct attcggagac tggaaggacg tggctccgct
    26701 tcttgaaggt ttggtagaaa atcaagatta tgttgtcgag caagatgctc gtaattctgc
    26761 agttcctttg ctagataagc gtgctatcaa cgctcgtatc gagccaggtg ctattatccg
    26821 tgaccaggtg gaaattggtg acaatgctgt tatcatgatg ggagctgtta tcaatatcgg
    26881 tgctgaaatc ggtgctggaa ccatgattga catgggtgcc atccttggtg gccgtgccat
    26941 cgttggaaaa aatagccacg ttggtgcagg tgcagttttg gcaggtgtga ttgagccagc
    27001 tagtgctgaa ccagtccgtg tcggagacaa tgttctcatc ggggctaatg cagtggttat
    27061 cgaaggagtc caaatcggta gtggttcagt tgtcgcagca ggagctattg ttacccaaga
    27121 tgtaccagaa aacgtggtag tagcaggtgt tccagctcgt attatcaaag aaattgatgc
    27181 ccaaactcaa caaaaaacag cgctagagga tgcgcttcgt accttgtaat tgtaaaagta
    27241 aaaaagaggc ggaacccttt ttccagcctc tttctgctat atcggaggac agatagatgt
    27301 tagatttgat tcagactaga cgagatttac accagattcc agagattggc ttggaggagt
    27361 tcaagactca ggcttatttg ctggatgtga ttgagaaatt gactacgggc aaggattttg
    27421 ttcaaattcg tacttggcgg acagggattt tggtctacct gcagggaagt cagccggagc
    27481 gaaccattgg ttggcggaca gatattgatg gcctgcctat cgtcgaacaa acaggtctac
    27541 cttttgcttc tcaacaccaa ggtcgcatgc atgcctgtgg ccatgatttt catatgacca
    27601 ttgccttagg ctgtcttgag cgcgcccttg aggagcaacc caagaataat ctgctctttc
    27661 tatttcagcc tgctgaagaa aatgaagctg gtggtatgct catgtatgag gacgatgctt
    27721 ttggagattg gttgccagat caattctatg gtctccatgt tcgtccagat ttgaaagtcg
    27781 gccagattgc gactaatact catacactct ttgcaggaac ttgcgaggtg aagatccgtt
    27841 tcaaaggcaa aggtggccac gcagcttttc ctcatgaagc caatgacgcc ttggtggcgg
    27901 ctagttactt tgtgacccaa gtgcagtcag ttgttagccg caatgtcaac ccaatcgagg
    27961 gagcagtggt gaccttcggc gtttttcaag ctggaaccac caacaatgtc atcacagaca
    28021 cagctttttt acatggaact attcgtgcct tgacacagga catgagtctc ttggtgcaaa
    28081 agagggtaaa aacagttgca gaaggggttg cagctgcctt tgatatggaa gtcgaagtgg
    28141 aactcaaaca aggtggatac ctacctgttg agaacaatcc agccttggcg cgtgaactga
    28201 tggacttctt tgacgagaaa gacggaatcg agttgattga tatcgaacct gctatgacag
    28261 gcgaggactt tggttatctc ctttcaaagg ttgatggcgt gatgttttgg ctaggtatcg
    28321 atagtcccta cgctcttcat caccctcaaa tgagtcctaa ggaagaagtc ttagctattg
    28381 gggtggctgc ggtgtctagt ttcctgaaaa agaaggcagc agaatagagg gcttgtctat
    28441 gaaatcggaa ttacgcaagc aagtcttgca tgaaatgaag gctttatctc aagaacaaaa
    28501 acaggctata gaccaagctt taaccgagcg aattttacaa cacccctttt atcaagaagc
    28561 caaggtcatc gcaacctacc tctctttttc tcatgagttt caaacgcggg aactgattga
    28621 gcaggcgctg aaggacggca agaaggtttt aatacccaaa acttatccca aggggcgcat
    28681 ggactttgta gtctatgatc tgcagcagtt ggtaaaaact gcctttgcct tactggagcc
    28741 acagggagat ttggaagtgg tggatgtatc tcagattgat ttgattcatg ttcctggcct
    28801 ggcttttacg acggatggat atcggattgg atatggtgga ggttattatg accgctatct
    28861 ggaacatttt tctggtcata ctttgagtac ggttcatcct tgtcaaattc aggactttat
    28921 acctgaaaac catgatattc ctgttcagga ggtattaatt gatgaaggaa atctttgata
    28981 gacgttaccc tgtgacgagt ttcttcctct tagtgacggc cttggtattt ttactaatgt
    29041 tggtcactgc aggcgggaac tttgacaggg cagatacatt atttcgattt ggagccatgt
    29101 atgggccagc tattcgcctc tttcccgagc aggtttggcg tctcttgtct gccatttttg
    29161 ttcatattgg gtgggaacat ttcattgtta atatgctttc actttattat cttggaaggc
    29221 aggtagagga gattttcggt tctaagcagt ttttctttct ctatctttta tcaggaatga
    29281 tgggcaatct ctttgttttt gtatttagtc ctaaatcctt agcagcaggc gcctctacct
    29341 ctctttatgg gctatttgcc gcgattattg ttcttcgcta tgcaactcgc aatccttata
    29401 ttcaacagct agggcaatcc tatctgacac tttttgtggt taacattatt ggaagtgttc
    29461 tgattccagg aatcagccta gcaggccata tcggtggtgc agttggtggc gcatttctag
    29521 cagttatctt tccagttaga ggagaaaaac ggatgtataa taccagccag agattaggag
    29581 cggtagtctt gttcgtagga ctcgccattt tgcttttcta caagggaatg ggaatgtgac
    29641 gaagttgaaa gaaaatttgt gtaaaataaa aagataaggc attttttgaa ccttatcttt
    29701 ttattttatt ttattttatt ccttctcagt caattctttt ccaagttgga tgaggtaatt
    29761 cttcaaatca tctttgactt gtgggtgttt gagggcgtag tcgatggatg ttttcatgaa
    29821 gccaaacttg tctccgacat cgtaacgagc cccattgaac tcacgagcaa atacacgttg
    29881 tgttttattg agggtgtcga ttgcatctgt cagctgaatt tcatttcctg cacctggagc
    29941 ttggttttcg aggatttgga aaatttcagg cgtgaggagg tagcgtccga taatagcaag
    30001 gtcgctagga gcgtcctctg gagctggttt ttcaacaaag gtttcaacac tgtaaagacc
    30061 atcttttcct tcgccttgcg gagcaataac cccgtaagca gatacttcgt catgagggac
    30121 tggcatgaca gcgatagtag acgcgtgggt acgctcgtag tcatccatga gttgtttggt
    30181 aagtggaaca gccttttcgt ctgtgatatc catcaagtca tcaccaagca taacgacaaa
    30241 aggttcattt ccgacgaaag ccttggcttg caaaacagca tctccgagac cgcgtggatg
    30301 agtttggcgg ataaaatgca gccgcatgtc agttgtttta tcaactagct tcaaaagatc
    30361 tgttttccct ttttctttga ggttatattc caattcgaag tttgaatcaa agtggtcctc
    30421 aatagaacgt tttgatttac cagtgacaac tagaatatct tcaatacctg atttgagagc
    30481 ttcttccacg ataaactgga tagttggttt gtctacgatt ggcaacattt ctttggcaag
    30541 ggccttggtt gctggtaaaa atcgagttcc tagtccagca gcagggatga ctgcctttct
    30601 aacttttgat gtcataaaaa tcctttctat agagggttaa gaccactcat tttctgcttt
    30661 aaattcattg ttcatgatgt cataaatggc atctttgata ttggttccgt gataaataac
    30721 ttggtaaata gcctgtgtaa tgggcatata gactccaagt tcttgggcta gttcataggc
    30781 tgctcgagtc gttgaaattc cttcgattac catgcccata ttagcttcta tatcagctag
    30841 ggattctcct cgtccgagag catctccagc tctccagtta cgagagtgga tggaagttcc
    30901 cgttacgatc aaatctccca caccagataa gccgctatag gtcaatggac tggccccgag
    30961 tgctacccct aggcgggtga tttctgctaa acctcgagcg atgatggctg ccttagcatt
    31021 atcaccaaat ccaagaccat gtaaagctcc agcaccgaca gcaataatat ttttaagagc
    31081 accagcagtt tcaaccccga taacatccgt attggtataa agtcggaagt agtgattact
    31141 aaatagcttc tgaacgtatt gagctgtttg taaatcttta gaagcagcag ttattaaagt
    31201 taggtcacgc acaatggtct cttctgcatg actaggccct gaaacaacga cgatatcact
    31261 acggagatgt tcaggaattt cttcttcaag aatggttgat aatcgtttat ggctatcagg
    31321 ttctaatccc tttgatgcgt gcatgatgat aaccttatgg tccaaggttt gtgcaacttg
    31381 ctgggcaaca agtcgtgtca cttttgttgg gacaacaaac aaaatcgcat ccacatcttt
    31441 caatgtttct gctaagtcgg tgtaggcaat gatattttcg tctagaacga catctttaaa
    31501 gtagtgctta ttagtatggt gtgtattaat ttcattgatt tgctcgggaa gatttcccca
    31561 aatacgtacc tcgtgtccat tgtcatttaa gacttgtgaa agggcggttc cccaagaacc
    31621 aggccccaag acggcgacgg tttgtttttc catgatttcc tccttgatag agttctttct
    31681 cttattctat catattctag gggattttgc acttgcatta ctggggtatg gtggaaaaat
    31741 acacaaaaac cgagacgaag ggataaactc ttagtcttta gttaaagtga gaagaaatct
    31801 gatagatagt ttgattcagt atatgcttat aatagaggta aacaactcag gaagttcttg
    31861 taggttgcga gagtttggcg aaaaaattaa aagattacgt ttggctaaaa aaatcagtcg
    31921 ttcagaattt tgtggtgatg agtctgaatt aagtatccgt caattaatta gaattgaaaa
    31981 tggagaatcc agaccaatac taacaaagtt aaaatatatt gctgaacgtt tgggggttga
    32041 agattacaag ttgatgccaa gttatataga gttggataag gaatacctag aattgaagta
    32101 tttcttgatg aggactccta catacgaaga tgaaactatc gcccaaaaga aagagagtgt
    32161 ttttgataag atttttgaag agtattatga taggctacct gaggaagaaa gatttatcat
    32221 cccaaattat tcatatctgg cactaacgaa ctacacagtt caaaaattac cagaaaagct
    32281 agttgaaata ctgtccttct ggtgattttg cttaatttct gatgttcgag acataattga
    32341 ggagatttgt cttgattttt tcctaaaaaa gggcagactc ctcccttggt ttgatgctcg
    32401 atttttaaaa taaggtgact tattctgtta ggcatgtttt cccctgccct ctagctctat
    32461 atggaacagg gagtatcaga tttatctggc agattgtatc aaataaggct tgaaactgtt
    32521 ttgaataggc gtatagactt gagaatttga gaatatgtcg tctagcagta gagacatatt
    32581 ttccggcaat atgaaggaag agacgtcgaa aacgcttgat agtcaaggac tttacttcat
    32641 caccagctag ctgctttaaa aagaggtaga gattataagc cagacagccc atcatcatac
    32701 gtacttcatt cttaatcatg gtcgaactat ctgtcttatc cccaaagaat cctgctttcc
    32761 tttctttgat aaagttttca gcatttcctc gttcacgata gaattgaaac aactcctcac
    32821 tatctacact gcttaaattg gtcaaatgaa cttttttaca accataaaaa agagtactta
    32881 atcgtactct tctcttttaa ggattagttg aattaattca aatccactag ttctcctgtt
    32941 tctaggtaga ctaggcgttc acaaatgtta gcaatgtaat cagcgaagcg ttccagatgt
    33001 cctatgatat aaaggtattg agttccattg ggaattgagg tttcttggtc tttcataagt
    33061 ccaatgattt cctttgataa ggcataataa tattggtcaa tctgttcatc tttttgagca
    33121 atactaatag cttttgaggc ttggtgcaaa ggaaaggcaa ccaataaatc ggctagcatg
    33181 ctgagggata atttacccat ttggtgtaac tgttcttcgt caggggctag ttgattttct
    33241 tttagttgca aaacagcttt ggcaatgcct gccatatggt ctcccatacg ttcaaggtct
    33301 gaacaagaag acatgatgct aatcacaaat cgaaggtcag acacttgtgg ctgctgcaag
    33361 gccaacaaac gggcacaggt caattcgata gcgctttgac cttggttgat agcatgatcc
    33421 ttattgataa ttagctctgc catctccttg tctttggagg ctaaggccag taaggctttt
    33481 gaagctgttt caaggacaag ttgccctagt cctaaaaagg attgttctaa ttcatgcaat
    33541 tctaagtcaa attgatttct cataggtttt tccttcctta tccgaaccgt cctgaaatat
    33601 agtcttctgt gcgctgatct tttggattgg taaacacgtc aacggtatct ccaaattcgc
    33661 aaatttctcc tgttaagaaa aaagcagttt tatctgaaat acgtgaagct tgttgcatgt
    33721 tatgggtaac aatgataatc gtataatcct tttttagttg ctgaatgagg tcttcaattt
    33781 ttaaagtgga gatagggtct aaggctgaag tcggctcatc cattaacaga atatcaggtt
    33841 ctactgctaa agctcgcgca atgcaaaggc gttgctgctg accgccagat aaggacatgg
    33901 cactcttttt aagatcatct ttgacttctt cccaaatggc tgcccctttt aaagatttct
    33961 ccactaaggc atctaattgt tttttgtctc gaataccatg tgtccttggg ccataagcca
    34021 cgttatcata gatagacatg gcaaagggat taggctgttg aaaaaccatc cctacacgct
    34081 tacgtagctg attaaggttg aatttgctac tataaatatc ttgctcatct aagaggactt
    34141 ggccttcaat atggcaagaa ggaaccaaat cgttcatccg gttaagggtt tttagaaaag
    34201 ttgatttgcc acaaccagat gggcctatca aggcagtaat ctgtctttct ggtaattgaa
    34261 tcgaaatatt ttttaaggct tgaaaatccc cgtaaaataa gtctaggtgt ctgactgaaa
    34321 atgttcccat actaggaagc tcctttcaca agttttcgag ataataagct tgatagagta
    34381 tttatcatta aaacagtaat aatcaaaatc acgccggtag cataggcttc attgacatgt
    34441 agcccctcac ttgacagcat atacatatgt agggctagag aacggcctga agacatgaga
    34501 ctacttggcg tattggtaga ggtacctaat gtatacatga gggcagctgt ttcaccaacg
    34561 atacggccaa tagctagtat cactccagct aaaatacctg gcatggcaac tggtagaaca
    34621 attctaaaaa cagtccgtaa cttacctgcc ccaagtccat aacttgcttg acgcatgcta
    34681 tcactaacag ataaaagggc ttcttctgtt gagcgaataa tgactggcaa caccatgata
    34741 actgaggtta agattcctga taacagagag tattgaaaac ctaagaagac tacaaagaag
    34801 agcatgccaa acagaccaaa aacaatggaa ggaatcccag ataaggtatc tgaggccaat
    34861 cgcatgattt taacacaaag ggaatctttt tttgtatatt ccacaagata aaaaccagca
    34921 aaaatcccta tgggcaaggc taaaagaaga gcaccaaaga ccagaataac ggtggaaata
    34981 atcgctggca taagggaaat gttctcagaa gtataagtcc aagaaaagag ggatagactt
    35041 agatgaggta agcctttgat gaggataaaa ccaatgatta aaaagagaga gccaaaggtt
    35101 aaagctgaaa aacaataaac gagaagtttt agcaggtatt tactcataag atgattttcc
    35161 tttcaagtag gcaaagtagg cattaatcaa gagaataagg aaaaagagaa ctgctgaggt
    35221 tgcaataagg gcttccctat gctgacctga tgcgtaagcc atttccagaa caatattggt
    35281 tgttaaggtt ctggttcctg aaaagagtcc acttggaata atcggctggt tgcctgccac
    35341 caaaataact gccatggttt cacctactgc gcgaccgatt cctaaaataa ctgctgataa
    35401 aataccagat ctcgcagctg gcaagatgac actaaaaata ctccgttcat gactagctcc
    35461 tagagccaag ctaccagaat aatacgtttt gggaactgtt cggatagcag attctgacaa
    35521 actgataatg gttggcaaaa tcattattcc taatagtaac gaagcggtta ggacactcat
    35581 gccatttcct aaaaagcttc taatccaagg caccaataat tgtaggccga aaaaaccata
    35641 aacaatagat ggaatggctg ccatcaagtt gatagctgat tttaagaagc catagacggg
    35701 ctttggacaa taataaacca taaacaccga tgtcaagatg cctgttggca ccccaatcac
    35761 aatcgctcct aaggtaatta ataaggaacc aacgatcatt ggtaaaatac catagcttgc
    35821 cggaatgttc gttggcgacc aatcactgcc taataaaaaa cgggcaaagc cgtagttagc
    35881 tatgaaaggt aagccattac taaaaataaa gaaacagatt agcaaaatag ctacaacagc
    35941 tactgttgca ctcatgaaaa aaattgccct aaaaactgct tctttgaagg cttgttttgt
    36001 cacatcttgt cctttctagt gaagaaagta agggagatac gacacctccc tacttgcctt
    36061 ctttatctta ttgtacgatg aaacgtctgc atctctttag agatttatgg agcaaacatt
    36121 ttatttaatc ttgtcccagg tggttaattt gccactaaaa acgtctgcaa gttcagccat
    36181 actgacttgg cttgccttat tgtcattatt gaccacaaca gcaataccgt ctaaagcaat
    36241 agcatcatgg gtgagactct taccttcttc aggagttaat tccctagaaa ccataccaat
    36301 atcagcggtt ttctccttaa cagcggtaat acctgctgaa gacccattag aggtaatatc
    36361 aatcgtaact tctggatttt cttttttata agcttctgct aatttttcca ttaaagaaga
    36421 tactgaagtg gaacctacaa cagacaactt gcctgataag tgttggcttg tatattccgt
    36481 ggtttcggtt ttagcttcaa taaatttatt atctgtgacc acttgttgac cttgtttgga
    36541 gtggataaag ctgataaaat cttgacctag cttggaaaga ttagaagacc aaacaatgtt
    36601 gaagggacgt tgaagagggt attcaccatc taaaactgtg tctcgactag ccttgacacc
    36661 atcaatctct aaagccttga cagatttcgt taaagatccc aaggagatgt agccgatagc
    36721 attagcattc ccttgaactg ctgagagaac accttctgta ctattttgaa tcacagctgt
    36781 tttggcagtg ttgtcaattt ttttatcacc gtcttttttg agaatccctg tgatttctgt
    36841 gaaggcaccc cgtgtcccag agccattttc tcgtgaaatc acctcaatcg ttcctgaagc
    36901 tgactgtttg gaagcagctg actgattgcc acaggcaaca agcccaaatc ctgataagcc
    36961 aatggctgca agagtaagca tttttttgaa tttcataata atcaccttta tctctatgta
    37021 tttttcttgt gtaggcttac tacatttata gtctaacaag tctttgtaaa ggtttatccc
    37081 tgattcatgt aaagattgtg taaagaatca aaaaaagcca cttttgaaaa atggctgctc
    37141 ctaaaaatag ctttaaaaat tattagtcct gtgcgaaaga ttggttagga agaaaaatcg
    37201 tgaagcaact gcctctgcca agctgactcg tcaccgtgac ttggccacct aataattgac
    37261 tgagttcttt gacaatggca aggccaagac cagtgccacc agtttgtctg cttcgacctt
    37321 tattaactcg gtaaaaacgt tcaaaaatac gatcctgctc taattgacta ataccaatcc
    37381 ctgtatctga tacagaaatc ttaatgcctt cgttcacctt ttgggtcttg acctcaattt
    37441 ttcccccttg ttcagtgtaa cggatggcat tggataaaag attgagtaag atttgggaaa
    37501 gtaattgact atctgatacg agggtgacat catctggcac ctgcaccttt agctgtaaat
    37561 ccttcttctt gagctgaggt tgcaagcttt gagtcaaatc ctgtacaaat tctgccaaag
    37621 aaagggtcgt ccattgtata ggcatttgtt gagccttaga taaggtaaga agatgctcaa
    37681 caatatgctc aagacgcaaa ctttctttgt aaataatgtc tagaaagtca tccttgagcg
    37741 cttcttcttc agctgacatc cccttaatgg tttcagcaaa gcccttaatc gaagtaactg
    37801 gtgtcctcaa ttcatgggag gcatttgaga caaaggctaa atttaacttt tcataagttc
    37861 taatcgttgt taaatcatat agcaagacga gcacagcttc cacagattgg gtggggctaa
    37921 aaacgggaac tgctgtcact tctaaaatca agtcaccctc atgaaaccca cttacttctt
    37981 gttttaacct tgttttttga tcaaaggctt ggtgaactaa attccgaata tccatccgtt
    38041 tgaggtcatc aagtgaactt atgtcgccgt ccacatcggg aaaataatga ggcagagagc
    38101 gactggataa taacatctga ccttgagcgg aaactaaaaa cgtccccatg gttaggtgcg
    38161 acagaagaac ctccattgtt tcggctagat ccttgtattg ctgatcctgt tgggagactt
    38221 tggtttttag gccagacaca tactgagcca aagactttaa gtcttcttgc cctttttcta
    38281 aaaagtattc actactggtc aagagaggtt ggtgcaaggt ctcaaaagca acttcccatt
    38341 tccaaaggca aaagagccag tagccaccta gtcccaaaga aagggctaga agaaagagac
    38401 cgatgccttt actgatccaa gttaatgcca tccctgcaat cagaatgagg ctaacactta
    38461 gattgactag ccaaaattga aggtagcgtt tcatctataa ctccttgaac ttataaccat
    38521 aaccccgaat ggttcgaata aattgagggg ctttaggatt gtcttcaatt ttttccctca
    38581 acttaccaat atgaacgtcc accaaacgtg tttcctgccc aaagtcatac ccccagatac
    38641 gttccaaaag acgctctcta gtcagtgtca tgttgggatg tttcataaga tagagcaaga
    38701 gttcaaattc ttttggggtc aaactcagta acttattcgc cttgtagact tcatgacgct
    38761 cagggtatac tttcaaggtc ccaaatagcc aagaatcgtc agcgatatta tctgaatcat
    38821 ctccttcttg ttctccttta gttcgcctga ggacagcctt gacacgcgcc agcaattctc
    38881 tagggctaaa aggcttggtc aggtagtcat cagcccctaa ttccaaggcc aaaaccttat
    38941 caaattcatc acttttcgca gaaaccatca taattggagt tttgacgcct ttggctctca
    39001 gccgcttaca aacttccatg ccatctaatt gtggtaacat gatatcaagc aagataaaat
    39061 caaagggttc tgtttctgcc aaagctaagg ccttccgtcc atttgtcacc aattgagtag
    39121 aaaagccttc cttacttaaa tggtagtcaa gcaatttcag aatgtgttct tcatcatcca
    39181 ctaataagac ttgttttgtc atctattatc tcctattggt aacattataa cacaattatc
    39241 agaaatccta acattgctaa atcagattaa atttgcctat caagactagt atctggtcaa
    39301 acgctcaatc atctccttgt gctctggata ggtcgccagt agatctaccc tttcaaataa
    39361 ttcaaaatcc tcaaattcaa aaccaggagc aacaagacaa gaaaccagag catcatcctt
    39421 atcaactgtt gatccccaaa tagtgccctt aggaacacag tagtgaagtt gttgcccttt
    39481 ggatatgtcc aggcctaaag tgactgcttc gtagtgacca tctgctgtaa tcatgtgaac
    39541 agtaagtggg gatcctgcat gaaaatacca gatttcatct gctgtcaatc ggtgaaaatg
    39601 tgaaggattc gtttcttcta ataagaaata aatactggta taaagcgccc ttcccttacc
    39661 agcaaggttt atagtgtctg aagctttttt tgtttgtcta aaatagccac cttcaatatg
    39721 gggagctaac tctagagttc ttatcaagtc ttctttatcc gtcggagcca atgggttgaa
    39781 gtaactcttg ttcaaagtgg ttttacgatt tcaagaactc ctctcagttc tgaggacacg
    39841 gtaatgattg atgcgacgga agtacaaatc aatcgcccta aaaaaagaat tagcgaat
//
LOCUS       DAVDWT010000020        39628 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVDWT010000020 DAVDWT010000000
VERSION     DAVDWT010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39628
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.020"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(<1..787)
                     /gene="lytB"
                     /locus_tag="VUW50_001185"
     CDS             complement(<1..787)
                     /gene="lytB"
                     /locus_tag="VUW50_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HET7983086.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGSFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLK"
     gene            complement(829..1767)
                     /locus_tag="VUW50_001186"
     CDS             complement(829..1767)
                     /locus_tag="VUW50_001186"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET7983087.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKVTNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            complement(1778..2578)
                     /locus_tag="VUW50_001187"
     CDS             complement(1778..2578)
                     /locus_tag="VUW50_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HET7983088.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            complement(2828..3208)
                     /locus_tag="VUW50_001188"
     CDS             complement(2828..3208)
                     /locus_tag="VUW50_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7983089.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            complement(3266..3625)
                     /gene="rplT"
                     /locus_tag="VUW50_001189"
     CDS             complement(3266..3625)
                     /gene="rplT"
                     /locus_tag="VUW50_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET7983090.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            complement(3677..3877)
                     /gene="rpmI"
                     /locus_tag="VUW50_001190"
     CDS             complement(3677..3877)
                     /gene="rpmI"
                     /locus_tag="VUW50_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET7983091.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            complement(3910..4440)
                     /gene="infC"
                     /locus_tag="VUW50_001191"
     CDS             complement(3910..4440)
                     /gene="infC"
                     /locus_tag="VUW50_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET7983092.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     misc_feature    complement(4456..4587)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(4751..5935)
                     /locus_tag="VUW50_001192"
     CDS             complement(4751..5935)
                     /locus_tag="VUW50_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7983093.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIVLALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASPGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKEFFN
                     RF"
     gene            complement(5939..6526)
                     /locus_tag="VUW50_001193"
     CDS             complement(5939..6526)
                     /locus_tag="VUW50_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7983094.1"
                     /translation="MLQLTHVTLKTRQVILQDVDFTFKKGRVYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSGLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDVCDRVVTIHQGQIEEV"
     gene            complement(6559..6747)
                     /locus_tag="VUW50_001194"
     CDS             complement(6559..6747)
                     /locus_tag="VUW50_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983095.1"
                     /translation="MTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSWSRYDSYSEYKVG
                     GGWNYARYEVINYYSGGY"
     gene            complement(6926..9166)
                     /locus_tag="VUW50_001195"
     CDS             complement(6926..9166)
                     /locus_tag="VUW50_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HET7983096.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMGNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     VFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLI
                     SLALVYDLRKNIKGLTVLSLLITGLFFLTKYPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIKKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            complement(9150..9800)
                     /locus_tag="VUW50_001196"
     CDS             complement(9150..9800)
                     /locus_tag="VUW50_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HET7983097.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDSSTEKEVKKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            complement(9868..10437)
                     /locus_tag="VUW50_001197"
     CDS             complement(9868..10437)
                     /locus_tag="VUW50_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7983098.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            10614..11713
                     /gene="ald"
                     /locus_tag="VUW50_001198"
                     /pseudo
     CDS             10614..11713
                     /gene="ald"
                     /locus_tag="VUW50_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003072563.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            complement(12080..12340)
                     /locus_tag="VUW50_001199"
                     /pseudo
     CDS             complement(12080..12340)
                     /locus_tag="VUW50_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(12343..12843)
                     /locus_tag="VUW50_001200"
     CDS             complement(12343..12843)
                     /locus_tag="VUW50_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7983099.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            12879..13061
                     /locus_tag="VUW50_001201"
     CDS             12879..13061
                     /locus_tag="VUW50_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983100.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLRPLDKTFSLYNWIFLSQKYI
                     RLVRNLVHFIIDDGTN"
     gene            complement(13026..13840)
                     /locus_tag="VUW50_001202"
                     /pseudo
     CDS             complement(13026..13840)
                     /locus_tag="VUW50_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
     gene            complement(13909..14877)
                     /locus_tag="VUW50_001203"
     CDS             complement(13909..14877)
                     /locus_tag="VUW50_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET7983101.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(14963..15178)
                     /locus_tag="VUW50_001204"
     CDS             complement(14963..15178)
                     /locus_tag="VUW50_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HET7983102.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEESAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            complement(15187..16041)
                     /gene="cvfB"
                     /locus_tag="VUW50_001205"
     CDS             complement(15187..16041)
                     /gene="cvfB"
                     /locus_tag="VUW50_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HET7983103.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            complement(16101..16658)
                     /gene="frr"
                     /locus_tag="VUW50_001206"
     CDS             complement(16101..16658)
                     /gene="frr"
                     /locus_tag="VUW50_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET7983104.1"
                     /translation="MANAIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDIGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKREKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            complement(16667..17404)
                     /locus_tag="VUW50_001207"
     CDS             complement(16667..17404)
                     /locus_tag="VUW50_001207"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET7983105.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQPGNIKRVVFGENIGTTVSNNIEEKE"
     gene            complement(17488..18822)
                     /gene="trmFO"
                     /locus_tag="VUW50_001208"
     CDS             complement(17488..18822)
                     /gene="trmFO"
                     /locus_tag="VUW50_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET7983106.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGAGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLSSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEAIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            complement(19016..19270)
                     /locus_tag="VUW50_001209"
     CDS             complement(19016..19270)
                     /locus_tag="VUW50_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET7983107.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            19265..20069
                     /locus_tag="VUW50_001210"
                     /pseudo
     CDS             19265..20069
                     /locus_tag="VUW50_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(20058..20704)
                     /locus_tag="VUW50_001211"
                     /pseudo
     CDS             complement(20058..20704)
                     /locus_tag="VUW50_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            complement(20813..21331)
                     /locus_tag="VUW50_001212"
     CDS             complement(20813..21331)
                     /locus_tag="VUW50_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HET7983108.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(21558..22427)
                     /gene="rsmI"
                     /locus_tag="VUW50_001213"
     CDS             complement(21558..22427)
                     /gene="rsmI"
                     /locus_tag="VUW50_001213"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET7983109.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(22430..22747)
                     /gene="yabA"
                     /locus_tag="VUW50_001214"
     CDS             complement(22430..22747)
                     /gene="yabA"
                     /locus_tag="VUW50_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HET7983110.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(22787..23677)
                     /locus_tag="VUW50_001215"
     CDS             complement(22787..23677)
                     /locus_tag="VUW50_001215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET7983111.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(23674..24312)
                     /locus_tag="VUW50_001216"
     CDS             complement(23674..24312)
                     /locus_tag="VUW50_001216"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET7983112.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLELHKKVRQGYLSLLEKEGNRIVKIDASLPLEQVVETTKAVLFDGMDLAK"
     gene            complement(24532..25329)
                     /gene="proC"
                     /locus_tag="VUW50_001217"
     CDS             complement(24532..25329)
                     /gene="proC"
                     /locus_tag="VUW50_001217"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET7983113.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFYQLLAKAGLLVELGES
                     LIDAATGLAGCGPAFVYLFIEALADAGVQTGLPREIALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMDAVHQAYKRTQELGK"
     gene            complement(25333..26595)
                     /locus_tag="VUW50_001218"
     CDS             complement(25333..26595)
                     /locus_tag="VUW50_001218"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET7983114.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAIGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            complement(26605..27735)
                     /locus_tag="VUW50_001219"
     CDS             complement(26605..27735)
                     /locus_tag="VUW50_001219"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET7983115.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(27815..29698)
                     /gene="cbpE"
                     /locus_tag="VUW50_001220"
                     /gene_synonym="pce"
     CDS             complement(27815..29698)
                     /gene="cbpE"
                     /locus_tag="VUW50_001220"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HET7983116.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSAYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGASAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYMGQW
                     YYFAPSGEMKMGWVKDKETWYYMDSTGVMKTGEIEVAGQHYYLEDSGAMKQGWHKKAN
                     DWYFYKTDGSRAVGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVSIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNFSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(29701..30588)
                     /locus_tag="VUW50_001221"
     CDS             complement(29701..30588)
                     /locus_tag="VUW50_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET7983117.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(30578..31039)
                     /locus_tag="VUW50_001222"
     CDS             complement(30578..31039)
                     /locus_tag="VUW50_001222"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET7983118.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(31036..31944)
                     /locus_tag="VUW50_001223"
     CDS             complement(31036..31944)
                     /locus_tag="VUW50_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7983119.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            31937..32134
                     /locus_tag="VUW50_001224"
                     /pseudo
     CDS             31937..32134
                     /locus_tag="VUW50_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(32186..32857)
                     /locus_tag="VUW50_001225"
     CDS             complement(32186..32857)
                     /locus_tag="VUW50_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7983120.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVIMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(32911..33132)
                     /locus_tag="VUW50_001226"
     CDS             complement(32911..33132)
                     /locus_tag="VUW50_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HET7983121.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            33404..34213
                     /locus_tag="VUW50_001227"
     CDS             33404..34213
                     /locus_tag="VUW50_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET7983122.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHIAAIHQAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(34394..35269)
                     /gene="aguB"
                     /locus_tag="VUW50_001228"
     CDS             complement(34394..35269)
                     /gene="aguB"
                     /locus_tag="VUW50_001228"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HET7983123.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(35279..36364)
                     /gene="aguA"
                     /locus_tag="VUW50_001229"
     CDS             complement(35279..36364)
                     /gene="aguA"
                     /locus_tag="VUW50_001229"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HET7983124.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFSD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(36361..37488)
                     /gene="nspC"
                     /locus_tag="VUW50_001230"
     CDS             complement(36361..37488)
                     /gene="nspC"
                     /locus_tag="VUW50_001230"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HET7983125.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLEIMDHIVF
                     NSERQLRKHGPRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(37488..38747)
                     /locus_tag="VUW50_001231"
     CDS             complement(37488..38747)
                     /locus_tag="VUW50_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HET7983126.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVKKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(38744..39604)
                     /gene="speE"
                     /locus_tag="VUW50_001232"
     CDS             complement(38744..39604)
                     /gene="speE"
                     /locus_tag="VUW50_001232"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET7983127.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
BASE COUNT        11450 a         9004 c         7247 g        11927 t
ORIGIN      
        1 acttgaggta aaaccaagat tccttatccc aaatccattc attggctgcc atgtagccac
       61 cggattttag ataataatag tgaccattct cgaaaatcca ttctttatca gcatagtttc
      121 cattttcttt gatgtaaaac caagattggt attgtttgtc aaaaagccaa ccttgctgta
      181 ctttggcacc actagcattc acataagctt gattaatcca ctgacttgtc agtagataac
      241 caccggattt gaaataatag tccttccctt taatttggag ccattctttc tctgcgtgct
      301 gtccatctgc tttgatataa aaccaagcat cgtattgaga atcatagacc cagtcttcta
      361 ttactttggc acctgttgca ccaacatagg aagttcctac ccaagcattt cttttcatct
      421 ttccatcttg gtcaagataa taaaaggatc ccttgtcttc tacccattct gatttggcca
      481 tatagccacc agatttgagg taaaaatagt cgtcaccttg ctttagccat tcattttcag
      541 catagttagc atctgctttt atatagaacc aagattgata atgagtatca aaaacccact
      601 cattcgctgc ttgactgcca tcttctttca gatactgttt tccttgccaa gtaccatcac
      661 tagccattgc accctctgga ctagctaaga aaaatagcag agctaaaaaa ataaatctta
      721 ctttcttcat taaccttctt cctctgttct tatttatttt attgtaccaa aattctccta
      781 aattcatatt acaaactaat aaaaaatcaa gaacagattg attgcagttt acctcagaga
      841 ctcttttact tcctgacgga gttcttccag actgctaata ccgtatttat ccatgacttt
      901 tggtaaattt tcgatgatgt cagggcaggc ataaggattg gtaaagttag ctgttccaac
      961 tccgatagca gatgccccag ccagatacat ttctagggca gcttcagccg aatccactcc
     1021 ccccattcca atgataggca ggtctgttgt ttgggctact tggcggatga gtttgagggc
     1081 tactggaaag actgctggac ctgacattcc acctgttcca ttggccaaga ttggttttct
     1141 ggttttgagg tcaaagcgca ttccaaccag agtattgatc atggtcaagc cacttgctcc
     1201 cgcatcttct gcagcttttg cgacagtaac gatatcggtc acactcgggg ttaatttgac
     1261 ataaactggc acttctgagg cttccacagc tgctttcacc acatcataag ccaaatctgg
     1321 atcttgacca atcaaaagtc catgattaca gtggtcaaca ttgggacaag aaatattgag
     1381 ctcgatagct tttacattag ttaccttgga aatcccatga gaaacagctg catactcttg
     1441 ttttgaaaaa ccagctacat tggcaataat aggaagattt ggatattctc tttccagcca
     1501 aggtagcttt tcagccaaaa caacctctaa accaggattt tgcaagccaa ttgcattgag
     1561 cataccagca ggcgtctctg ccactcttgg agttggattt ccaaaacgtg gttcaagggt
     1621 tgtcgccttg atcataatag aacctaaaag gtctaaatca tagtacttgg catactcttg
     1681 tccaaagcca aaacagcctg atgctggaat aatcggattt ttcaaatcca aaccaggtag
     1741 agaaacttgt aatcgatttg tagtcataat tttctcctta taatacaact gttcctgtgc
     1801 ggaaaacagg accatcttca cagacgcgtt ggctgaccgt ctcgctttct ggtactttta
     1861 gaacacaggc atagcaagct cccatcccac aagccatacg agattccaga gataaatagg
     1921 ctcttgggtg atcatcaaag gtttgattga tatacttcat cattcctgga gccccacacg
     1981 agtaaacagc atcaaactga ctgtctaaat cattgataac aacggaaaca tttcccttga
     2041 tgccataaga accatcatct gtcgttacaa agacctgacc atactgagcc aattccgttt
     2101 tcaaaataac agcatcctta ttagcaaaac cgaggactgt cactactttc actccacgtt
     2161 catgcaattc cttggccacc tcaagcaagg gtggaacacc aatcccacca ccaacaagga
     2221 gaacctgatt ctgctcatca aggtcagaca agtcaaaacc atttccctga ggccccatca
     2281 catcaagagt gtctccctga cttaaggttg aaaaaattgc agtcccagct ccctcaatcc
     2341 gataaatgag gtgacactgc ttgtttgcct tgtcaataga cgaaattgaa ataggacgac
     2401 gtaagagatg ggcatcgtct ggtacacgca gatgaagaaa ttggcctgct cgcatggctt
     2461 caaccatttc tccttctagg actaattcaa agattgctgg cgcgatttcc tcttgtgcaa
     2521 ccaccttcat ggtttccaac cgaatgacac ccaaacgctt cttacatgtg agattcataa
     2581 cttttcctcc ttaaatttta aggggaccag ataaagaaaa gaccttgcta gtgcaaggcc
     2641 tcagttaaaa tcgtacaaca caagagagca cactcaaaag gtctcctcta gaaaattgca
     2701 ctattctttt atctgacacc ttgtgagtct ctctggactc cccttaaagg ttgaattttt
     2761 attagtatac tctttcagct aaaaaaagtc aagtagaaaa cgaatattct acttaacttg
     2821 cacgaaatta tttttcacga atgacttcga ccttatatcc atcagggtct ttaacaaagt
     2881 aatagtttgg tgtagttcct ggtagaccat ttggctcagt cacttcatag ccttttgcac
     2941 tgtgttcttg atgaagtgcc tcaagatcag gtgtactgag ggcgatatgg gcaaacccat
     3001 caccaaccac atacggaccg tgatcgtagt tataagtcaa ctccaactca taatcatcac
     3061 cctcaagacc tagatagaca atcgtgaagg catggtctgg aaaatctctg cgacgcaatt
     3121 ctttaaaacc aaaagcatct tgataaaatg cgattgattt ttcaagattt tctactcgta
     3181 agcaagtgtg tagcattttt gaagccatat ctttctcctt tatttttaaa aagactggac
     3241 aatcctgttc cagtctcatc agttgttatt taccaagttt tgctttagct gcatctgcaa
     3301 gagctgtgaa agctactgca tcgttaacag ccaagtcagc aagcatttta cggttaactt
     3361 cgatctcagc caatttcaaa ccatgcatca attgtgagta tgaaagtccg ttcatacgag
     3421 ctgccgcatt gatacgagta atccacaatt tgcggaagtc acgttttttc tgacgacggt
     3481 cacggtatgc atagtagtaa gagttcatta cttgttcttt tgcagtacgg aacaagatgt
     3541 gtttagctcc atagtaacct tttgctaatt taagaatacg tttacgacgt ttgcgtgata
     3601 caacgccacc tttaacacgt gccatgtttt atttcctcca aatatttcct agaattgttt
     3661 acttacagtc aagctattat ttcaagcgag taagcattgc tttgatacgt ttgtaatctc
     3721 ctgaatgcac catagatgct ttacgaagat gacgacgttg tttcttagtt tttccgtgga
     3781 aacggtgaga agtgtaagca cggaaacgtt taagtccacc agaacctgta cgtttgaaac
     3841 gtttagctga tgcgcggtgt gttttttgtt ttggcatgat tttttctcct ttatttaact
     3901 ttctgacaat tattttttgt cagtcgctgg cgccaattgc atgaacattt gacgtccatc
     3961 cattttagca cgttgttcga tgatggcaat atcttgagtt gcttcagcaa actcggctaa
     4021 aacttttgca ccaatctctt tatgggtaat catacgaccc ttaaagcgaa tagatacctt
     4081 aactttattt cctttttcaa ggaacttacg tgcattacga agttttgtat caaagtcacc
     4141 cttgtcaatt gttggactta gacgaacttc tttcacagta acaacgcttt gttttttacg
     4201 ttgttctttt tgcttcttct ggtactcaaa tttgaactta ccgtagtcca taatttttgc
     4261 aacaggcggt ttggcttggg gttgaatcaa tactaggtca acattagcgt tatcagccaa
     4321 agcttgcgct tcactgagtg gcttgatacc tagctgttct ccttcaagac caatcaagcg
     4381 aacttcacgt acacgaatct catcattgat gaataagtct tgctttgcta tggttttcac
     4441 ctcttttgtt ttattagaga aaaacaatag cggacttgta aatatacaag cccgcacgtt
     4501 atgatagcgt ttcttcgaaa cttttcatcc gtagggccag gcaacttaat gtcacaaggc
     4561 gagaagctct cacttctgct tttctcaact tttatatgat accaagtttt ctagctcttg
     4621 tcaagataaa aagttatttt ttgaaaagtt tgtgtttgta ttttggtttg gttttcgaat
     4681 caaaaagaga gaatcaagtt gattccctct ctatgttagt ttttacttat tttccctacc
     4741 tataggaaag ctagaatcta ttaaaaaatt cttttttctg tgaacttccc catctttcaa
     4801 taaatagaat tcccaatagc aaaaagataa tcaggcaagg aagtaagacc attcccatgc
     4861 tccaatttag atcgacatta tcaatctgct taggtaatct tccagataaa atctccactg
     4921 aacgcaagta agtaaaggga atcagatgtg caatcctttg aagaggctga atggtttgga
     4981 taccaaacaa taagccaaca atcccaatga gtgaaagaaa gaggacaggc attttttgct
     5041 tgaaaaagta agcaatcaag tacacaactt ccacaatgac gataaaggct aagaaagcta
     5101 agagcaagcc aggaaataat acatcttgta ttttcccaat agttacttct tgattcacta
     5161 agctataaat tgggtaggga taatctaact gtccaaaacc acttatcaga cttcccacta
     5221 gaaaagaaaa gccacagatt ccgataaaca gcacagttac atatcccact ccaactccaa
     5281 gagaggatat tgcaaatgtc acttttgaaa caggatataa gtgagctgtg tccagatgat
     5341 tttgatatct ttctgcaaat agttgtgtta gcataaaaat aatagcaacc acaaacaaac
     5401 ttgggatgat aacctctaaa atccagacaa tctgatcaat cccgtgggtc ggaaactcca
     5461 aagtatgtgc ttttatgttc aagggataca gggcttggta aatcttccgt tcgcggtcaa
     5521 cccccatttt taagccaggg ctagcagtcg ggtcatttga tacaaattca taattcttct
     5581 cttcatcttg ccactgcaaa tagtaggctt ctttccagcg cccttctttt aataaagtca
     5641 gaatttctgt ctttcgcgtc aaaagatttt tttgcacgtc taaattattt ttagcaaact
     5701 ggtattcctc cgagctggta tcagacattt gggagagttt ctcttcattt tcattgatag
     5761 ccctctcgtt ggctgcaatg cgactttcca acctgctctc caagctgtgt gagtttgcag
     5821 tctgactatt taaataaaag gtaacaccga gtacagatac aaataaagct aagacaatcc
     5881 agtttaagcg gcttttgaaa acttttttca ataaaaatag actaacatct ttcataaact
     5941 aaacctcttc tatctgcccc tgatgaatgg ttactactct atcgcagaca tcaaccaact
     6001 cttccttata gtgggaactt aaaagaacca gctgttcttg tctatcgatt tgtgctagcc
     6061 tatcaaaaaa cttctgtcga taatactcat ctaagccatt tgtaatctca tccatgagcc
     6121 agcatttggc ctgactgagg aaatacatgg caatcaccaa gcgttgcttc atgcctaagg
     6181 aatacttgcg aatgggaaga ctgatatagt cagacatttc ccaataggcg atttcatccc
     6241 tcaagttcag acctgacttc cagatgtttt tgataagacg aaggtagtcc atcccactta
     6301 agtttccatc cagccattca atactctcat aataaaataa agaaggaggg gctgcgatat
     6361 ttccactact tatgggaatt aaattgctaa tggcacggaa cagggtcgtc tttccagagc
     6421 cattgatagc aagaagacca taaaccctac cctttttaaa ggtgaaatcc acatcttgca
     6481 agatgacttg tcgcgttttt aaggtcacat gagtaagctg caacatatct agccctcctt
     6541 tttctcactc tttaagaatt aatagcctcc gctgtaatag tttatgacct cataacgagc
     6601 gtagttccag cctccgccaa ctttatactc agaatagctg tcataacgag accatgacgg
     6661 aatccaagca tactgatggt cgtgatagtt ggtactatat tcccaaaccg tattccaatc
     6721 atacttgtaa cttttagtga ctgtcacagc agatacactg gactgaagaa gaccaataga
     6781 ttataaacta actaataaaa caacttttgc tgatttttaa tgattttata tcctcaatat
     6841 tgaatacgtt ttcattatac catttttatc attattatgc aacaaatttt tagtttattt
     6901 cactaatcta caacatttat ccttcctatc gaacactttc gattttccaa ctatccaacc
     6961 ccttaaaacg tatagctcct tgctggtcag ttcgataaac tttgctattg ataccttcca
     7021 gtcgtgtcaa tgtttcctga tgggggagtt tcattcgatt gctctttcca actgagataa
     7081 gagtaagctc tggtttgagt ttttctagaa aggctggact tgatgatttt ttattgccat
     7141 gttggctagc tttcaaaaca tttactttca agtctggata gtgcttcagc aagtccttct
     7201 ctcctttctc ctccaaattt cccgtgaaga gaaattgctt atccaagaat ttcccataca
     7261 gaactagggt atcatcgtgt cctccatctc ccattttcct tggagataga acttctaact
     7321 gacttccaaa aatgggcaag ttctccccta ctatcatact acgcaccttt gtttgagtcg
     7381 cctgtagttc tgccacaaat tccttctgtt tcagactgtc ttttgatact agaatctccc
     7441 ctacatggaa agccttggtc atctctgaca aatctccaac atgctccttg tccgtgttag
     7501 tcaaaattag ctggtcaatc ttagctactc ctcgactttt gagataggga atcaaggttc
     7561 gctgggcatt gctggtcgtc atcttttctt gccatttttt gattttctta taagattctg
     7621 ccttaccacc tacatctatg agaatggttt tcccagttac atcccgtagg aaaatacttt
     7681 ctccttgccc cacatccagc atggtgattt cattttccag tggatacttg gtaaggaaaa
     7741 agagacctgt aatcaataaa ctcaatactg ttaatccttt aatgtttttc ctcaaatcat
     7801 agaccaaagc caaggaaatt aacaataaga ttaaaagcca tgcgttgggt tgaccaaaga
     7861 caagtggtct ccttgccacc tgcgagacca agcgaataat gccctctaac cattcaaaga
     7921 taaagttcag ctgaatgact ggatagagaa aggaaaggac aaataagata gacaagagcg
     7981 gtaagaagac caagtcaaaa agaaaggaaa agacaaaggt caaaaggata gaccaaggtt
     8041 gaaattccgc aaaatagaag gatagaatgg gcaatatgcc caaggagatg actagacttt
     8101 cactagtaac agccttgagc ccctcccctt ctttgctggt catggtcagg ataaaagcat
     8161 aagcgcagga caagactcct cctgctgtca agaaaaagtt tggcatgaca ataaagagga
     8221 caagcaccgt caaggcaaaa ttatccaagc ccttaacccc atgttgagcc agtagctttt
     8281 gcaagagact gcgaataacc gatgctgaaa atccagttag tcccgcatag ataagggaaa
     8341 agggataagt cagccatttc aacttttctt gggtcaagcc caatcgcaag agaagtttct
     8401 taaatccatt catgaaaaaa cctacctgca tgccagatag ggcaaagagg tggataattc
     8461 ctagactgga ataaagctca ttcatctcct caaagtcggt gtccagatgt cccagcaaga
     8521 gtcctgtcat gtaattgccc atagggtctg gaaagtgcgt cttaatccaa accacagcct
     8581 ttcgacgtaa actggacaag ttttctccta tatcccaact gccaatcttt tgaagtgact
     8641 ggattgtttt gatattgaga gtctggtaaa ttccctgagt cttcagatag gcttggtaat
     8701 taaagccacc aaaatttctc tgcccttctg gctccgaaag cttcccttct agtcctatct
     8761 catgcaggtc agttaaagct tgaaaggctt ctttctcctc ctcggactgg agtttataat
     8821 agacttggaa agcacgaccg ttagacttgc cacgaaagga tagactatca ccattaacct
     8881 taatagtatc aggcaaaatc cgtacccttt caacagaatc cgccagattt tgactcgctt
     8941 gactctgttg ccaattttga aaaacaaacc aaaatccaaa gattccgcaa attattagaa
     9001 ctttaccagc agatttccac ggaaattgga taaagagaca gactagcaga aaaacaaagc
     9061 ccaacaaagc aagataagat gctgagaaaa tagcgtaata aagccaaagt aatagaaaac
     9121 tcaggtaaat taggggaata gagaaattct taatccactg taacatagtc tttaagcttt
     9181 tctattgttt tgccaccaat gccagagacc ttcttgagct cgtctactga cttgaacttg
     9241 ccatttgcct cacgatggtc aataatgtcc tgagctcgtt ttcctcccag tcccttgacc
     9301 tgcttgagtt cttccagact ggccttgttg agattgacct tcttttcctt gcttgttgaa
     9361 gaagctgtcc ccgaaccagt ctgttgacta actgcttctt ctcccttagt aggaacgtaa
     9421 accagagcct catcactaac tttctgagct agattgagcg acttgctgtc tgcttgctct
     9481 gtcaagccac cagccttctg aacagcatca ttgactcgac tacctacagg caagtcataa
     9541 atccctggcg atttgacagc acctttgaca tctactgtga ttagatcttg ttcaaggggt
     9601 tcttcctttt cttccttctt cacttccttt tcggtcgatg agtccttgga aacagctgca
     9661 acttcagcct gcaaattcgt ctctttgaca ggtgtttgtg gagctggttt tagcaggaaa
     9721 aatcctccta caagcaagcc cagaccagta cagatgacga tgattttata ctctttgatt
     9781 ttctcgataa ttgcttccat attttctcct ctcttagatt attcgtaaga ggaagaaaaa
     9841 acagtcgaaa aatttctttt caactgctta tttatttgca aaatagtctt ccttggtcaa
     9901 gacataatga actcttgtca cgattcggcc tttttcatgc tggtccatac aagcatatgg
     9961 ttctgcatgg gaaaaacgca tgcctgattt ctccatgacc tttcctgacg cgggattagc
    10021 cttatcgtga agggcagtca acttattcat ccctatcttc tcaaaagcta gctcaatcac
    10081 agcacgattg gcttctgtcg ttaatccttg attccaatac tttttattga taatgtagcc
    10141 aatagctgcc ttcttaagaa cagaatcaat cttgtgcaag tcaatggttc caataaactg
    10201 accattgctt tttagttcta ttccccaacg tcccaaggga ttagccaagt agaactgagc
    10261 aatgttattc ttggtttctt ccaagctttg attggttgga aaagtgtaac gtgtattacc
    10321 cttgtccgag gcatagtcaa acattgcttc cgcatcatcc aaagttacag gtctgagcaa
    10381 taaacgctcc gtttctatag acggatactg ggcaaatttc acaaatattg attccatact
    10441 tttccctcca ttagagcttg attttcacta gtctagcata aaaaaatttg gatgacaagt
    10501 ttttcttgat ttttgaagcg gtttcatata caatgaaacc gtcacatttt tgatttatga
    10561 actggaatgt gacaaccaaa acaagaccgt caattgaaag gtaggttatc tcaatgttaa
    10621 tcggaatccc aaaagaaatt aaaaataacg aaaaccgtgt cgccctcaca cctgcaggtg
    10681 ttcatagctt agttagtcgt ggtcatcgtg tccttatcga aacaaatgct ggtctcggtt
    10741 ctggctttac tgatgctgac tatcaaaagc aaggagctga gattgtcgct actgctggtg
    10801 aagcttgggc agcagagttg gttgtgaaag taaaagaatc tttaagttct gaatacggtt
    10861 acttgcgcga cgatcttctt ctcttcacct acttgcacat ggccgctgct ccagaattag
    10921 cagatgctat gttaacagca aaaacaactg aaactgttcg tgacaatcaa ggacaactac
    10981 cgctcctcgt tcctatgagt gaggttgcag gtcgtatggc tgttcaaatc ggagctcact
    11041 tccttactaa gcaagctggt ggctctggtg ttctacttgg tggtgtacca ggtgttccaa
    11101 aaggaaaagt aactatcatc ggtggtggtg tcgtcggtac acatgctgcc cgcatcgccc
    11161 ttggtcttgg tgctcaagtg actattttag atattagttc caagcgtctc tcagttctag
    11221 aagaagtctt tggaagtcaa attcaaactc ttatgtctaa ttcattcaac attgaagcaa
    11281 gtgtgagaga tgctgatgtg gtgattggag ccattctcat ccctggtgca aaagcaccgg
    11341 aattggtgac agatgagatg gtcaaacaaa tgcgtccagg ctctgtatcg ttgacgttgc
    11401 tgttgaccaa ggtggcgtta tcgaaacagc tgaccgtgtg acaacgcacg atgaacccgt
    11461 ctatgaaaaa cacggtgttc tccactatgc cgttgccaat atccctggtg cggttgctcg
    11521 cacttcaacc atcgccctaa ccaatgtcac tcttccttat atcgaagctt tggctggcaa
    11581 aggattcgca caagcaatct ctgaagatga aggcttgcgt caaggtgtga ctacttatca
    11641 aggttacttg actaacctac cagttgctca aggacttaat cgtgactaca ctgatatcaa
    11701 tgatttagta taaattttga aaagaaaagg gtgacttcgc aagtcctacc cactgtgctt
    11761 tgtgtcatca gctattacat cgttggttta ttgtactgta tactttgcaa ctggagactt
    11821 tcgttccaga ctcaattgtg cataagaaac tctaaattga ataaagtcaa cagagtttcg
    11881 cccaaccact tcgtcttgta atgacaatta tataaaccaa aggaagctga gctctttagt
    11941 cccagcttct ttttatatat aaaattttac ccgtgcaaag acagggcctt agcagacttc
    12001 ttttttactt cgttcaccct tgctttttct ttttaggttt gggcgttggc agttggttat
    12061 acatagctaa aattaggtct tatagaaaca tcttattatc aagttcttcc actcaaatca
    12121 tttctttggc acctttgtat ggaaactcaa aagaagattg gtcaatctta tctaagactg
    12181 cttgcacggg tttaactaaa aagcgatcgt cataaatgcc gccaataatc ttgccgcgga
    12241 agtaaagaat atactccccc atcatggaac ggtaagtcac atcatctaat cctgataatt
    12301 gttccaaaac aaattccaaa tagttcttac ttgatgccat ttctaatctt ctaggctctg
    12361 ttcagcgata acaaccgtat agagttcttg cttaacctcg catccaattg atttaaagcc
    12421 ctgcttttcc caaaaatgct gagattgcgg atttccctta acataagcca aacgtgcctt
    12481 tcgaaagttc ttagcaaaat aagctagtgc ttctgtcaca atatgactac caatcccttt
    12541 tctctgatag gcttgatcaa ccataaacaa accaataaaa acagtctcct catcaggata
    12601 tgcatagaca aaatccataa cagccacaag gtcagatcca ttccaaaatc caacaaaaaa
    12661 cttatcagcc ttagctttac cttcaggtag acaaagcatg tcctctttta cagttgcaaa
    12721 atttggctct ggtggacaat gctgaaaata cagaggatta ctttcatata aagataaaat
    12781 acttggaata tccttttcag ttagtatcct acaactgtaa tacttagata gttggtcaat
    12841 catcttttca aattcctact ttcttgtgcc ctgtgattat gacacaggaa gatgcactga
    12901 tcgtcatcag ccacataaaa gttctttcca tcgtgcctaa tcgttgtctc agacctttgg
    12961 ataaaacctt tagcctatac aactggattt tcctctctca aaagtatatt agacttgttc
    13021 ggaatctagt tcacttcata attgatgatg gcacaaatta attccgcccg taactcgaaa
    13081 cgttttctgc ggttacgata agggactgac atgatttgga aggtcttgaa tttagcgtta
    13141 aaatgttcaa tttcaattcg tatcgctgac atctctttat ttaactgctt atcatcctca
    13201 ctcaggcggc gatttttgga atttttagca ggaatgaaag tattctcatg aaatttcaag
    13261 atgcctaaat aacctaggtc aacaaaggca agcgtcgttt caggcaaact ttgtccaata
    13321 ctttctttga agagagtaaa atcatgcgta tgtccgtcag aaaaggccat ttgacagact
    13381 ttatgtgtcg tcaaatccag cataatttga gtttttaagg tgtgtcgttt ctttttacca
    13441 gaataatttt tgctttggtt ttgtttggac gctgaatcgg actttcggtc acgtcaatag
    13501 ccacagcagc actcggggct tctaaatggt ccaaatcaaa gctacctgac gcacgaagtg
    13561 tatcctccac ccaagtgatg atggcattta ccgtagctac accgacgcca aaatcaaagg
    13621 ccagcagacg ctgagtggga taatatcgca agtaacggag ggtcataatg agctggtctt
    13681 ccatgggcag actacgtgga cgtccacctt ttcggtgctg aatctgatag gctgaattca
    13741 ggtgttctag catgagttga aaggtttcct ttttgacacc aatcaatagt ttgaagtttt
    13801 ttgagttaag tttttgtgct ttgtcgtata ctgtttccat aaaaatagta taacactttt
    13861 tacagttacc gaacaagtct attcttttgc aggcgaactt cctcaaaatc agtcgtgtgc
    13921 aacttcagta gaatattcat aggctcggat aatctgagcg acaacaggat ggcgaaccac
    13981 atccttggct gaaaaatgaa caaagtcaat ctgatggatg ttcttgagtt tctcttgagc
    14041 atcaatcaaa ccggacttga cattacgtgg caggtcaatc tgactaatat ctccattgac
    14101 aatcatctta gaatgaaaac ctaaacgcgt caagaacatc ttcatctgca tgatggtcgt
    14161 gttttgcgcc tcatcgagaa tgacaaaggc atcatccaag gtccggccac gcatataggc
    14221 aaggggcgca atttcgataa tttcacgctc catgagacga gtcgtttggt ctttcccaag
    14281 aatttgatac aaggcatcgt aaacaggacg aaggtaagga tccaccttct ccttaagatc
    14341 acccggaaga aatccaagac tctctcccgc ttccaccgct ggacgagtta ggataattcg
    14401 cttgacttgc ccacgtttaa gggcagtcac tgccaaggtc actgcaagga aggtcttccc
    14461 tgtacctgct ggcccaattc caaaggtcac atcatgctgt ttgacactgt ccacataaag
    14521 cttttgccct agggttttga cacggatagg tttcccagta ttatctttga taatttcttc
    14581 ttcgtaaagg gcgacaaact tgtcaatttc atcatttttg accatgctaa tcgcagtgac
    14641 tacatctggc gtaccaacgg tcatcccacg atttaccaag accatcaaag cctgaataac
    14701 ttgacgggct tcctcacagg cagactcttc tcccaaaacc tggacaatct ccgtacgagc
    14761 atgaatcaca acatcaagct cttcttccat caaacgaaga tggcgttcat tggaaccaaa
    14821 aagatgaaac aggtcatctg gatgactcag ttgaatgtct attgaatgtt ccttcaaata
    14881 aagaacctct ctaatccttt tatttctttt tattatagca gagggatgga gaaattacta
    14941 gcccaaaccc aatgaataaa tgctagtgtt ctagatattc ctgccaaatg ctgtcaaaat
    15001 ctcctaggtt aaaagagaaa ctggcatgct cctccaaaaa gcgactgacc tcatcaaaat
    15061 catctgtgtg ttttggaaag gctgactctt caaaagcgag gtctgccaaa attgctttgg
    15121 gactgttact tttaggattg cgctcggtca tgagccaagt gtaaaatgat tttctcataa
    15181 gcctccctaa atcaactctg tcccaaactg gtcctgcttg attttaccag ccttcataag
    15241 accacctaaa gctttcttga actgaccttt agaaatgcca aaggttgcct tgatgtcgtc
    15301 tggagatgac ttgtcattta aggtcatgaa accgccattg ctttccaaat aagtcaaaat
    15361 catctgagca tcgttttcca acatttcaaa ggagcgtggt ttgagggaga ggttcagagt
    15421 gcggtccact tcacggaaac caataacgcg cgcatctaat acttgcccca aacgtggctc
    15481 tgcgtaacgc tcgctaggat gaataaaacc aagcatatta ttttctggta ggtaaacaaa
    15541 agttcctgac agcttgagac ggtaaacaat ggctggccag ttttggttct gcatgttgtt
    15601 gtaggcagga cgagcaagac gttggaagtc ttcttgataa gccaagaggc cccagatacg
    15661 gtctttctta tccacttcaa gacggatgta gagttggtcg cccttcttag gccagagttc
    15721 cttgagctca gggagaatat cgagtgacac aacgatttcc ttgtcaggaa ggcctgtatc
    15781 cacaaagaca cccaagtcct tacgaacctc tgtgacacgt ccccaaccaa attggtcctg
    15841 agtggcagtc acttctaagg ttgtcaggcg gagtttttgc ttcatatccg tgtatgcaaa
    15901 acctttgacc gtatccccta ctgtatgttg gccttcttcc ttagcaagag cataggtttg
    15961 accatccttt tgcacaaagt aaaaacggtc gttttcatcg atgatcagtc caacgataaa
    16021 acttgcaaga tttgtattca tatttccttc tttcgaataa aactcagcca gcaatgccaa
    16081 ctgagttttt ctgtttattt ttagacttcc aaaagttctt tctctttgtt agcagtcatg
    16141 tcgtcgatgt gtttaacagc atcgtctgtt actttttgaa tgtctttttc aagagtcttc
    16201 aattcgtctt cagtgatttc ttttgctttt tctcgtttct tagcttcgtc catagcatcg
    16261 cgacggatat tgcggacagc cactttagca ttttcgccga ccttcttcac ttctttagca
    16321 aggtcacgac gagtttcttc tgtaagagct gggataacca agcgaatcac agaaccgtca
    16381 ttagccggtg tgataccaat atcagaagcg ttcaaggcac gttcgatgtc tttcaatgaa
    16441 gacttgtcaa atggtgttac caacaaaaca cgcgcttctg gaatcgtaat tgaagcgatt
    16501 tggttaagag gagtttcgac tccatagtat tctacatgta cacggtcaag caagcttgca
    16561 ttggcacgac cagcacggat accaccaaat tcacgagcaa gtgattggtg agactgggtc
    16621 attctctctt tagctttttc aataattgcg ttagccatat tctttcttat tccttttctt
    16681 cgatattatt tgaaactgtt gttccgatat tttcaccaaa tacgacacgt ttgatgttgc
    16741 ctggttggtt catgttgaat acaaccaagt caatgtcgtt gtccattgag agggttgaag
    16801 ctgttgagtc catgatacga agacctttat tgataacgtc acggtgggtc aattcttcaa
    16861 acttaacagc tgtcttatct ttcttaggat cggcattgta aacaccatcg acaccatttt
    16921 tagccatgag gatggcatct gcttcgattt cagctgcacg aagggccgct gttgtatctg
    16981 tcgagaagta aggtgaacca attccagcac caaagataac gatacggcct ttttcaaggt
    17041 gacgaagggc acgtccacgg acataaggct ctgccacttg ttgcatggca atagctgttt
    17101 gaacacgtgt atcaacccca acttgttgca atgaatctgc catcacaaga gcattcataa
    17161 cagtcccaag cattcctgtg taatctgcct gaacacggtc catacctgct tctgctgcag
    17221 gttctccacg ccagagattt cctccaccga taacaagggc aatttcgata cctaagctat
    17281 gaacttcttg aatctctttt gcgattgttt gaactgtttg gatatcaatc cctacgccac
    17341 gttcaccggc aagggcttca cctgataact tgattaaaat acgtttatac ttgggattcg
    17401 ccattttcac tctccttctt tcatcctacc tattttatca caatttctaa gatttttata
    17461 gtatcatgag caattctttc aaaaaaatta gacagtcaaa aattcctcta agtcggcaag
    17521 ggcacgctct gcaatttttt cataacgagc cttcttatca cggatacgct cgccttccaa
    17581 ctccttgatg atcccaaaat tgacattcat tggttggaaa tgtttgctgt cggcatgggt
    17641 aatgtaatga gctaagcttc caatcgctgt cgtctcgggg aaaatagcct cgctttcttc
    17701 cttgaagaga cgagctgcgt taattcccgc aactaagcct gaagccgccg actcaacata
    17761 gccttccaca cccgtcattt gaccagcaaa gaagagattt ggttgtttct tagaacggta
    17821 agtctgctca agaagatttg gtgaatccat gtaagaattg cgatgcatca caccataacg
    17881 gacaaactcc gcattttcaa gacccggaat catttggaag acacgctttt gttctcccca
    17941 tttgaggtgg gtctggaaac caacaatatt gtagaggcta ccagctgcat tatcctgacg
    18001 aagttgcaca accgcataag gtgttttaaa ttctccatca cgaggtcctg tatagtcgtc
    18061 tgggtactca agaccgactg gcttcatagg gccataaagc atagttttaa tgccacgttt
    18121 ggccatgact tcgataggca tacatccttc aaagtacttt tctttttcaa aagaactaag
    18181 cggtgcttct tctgcattga ccaaagcttc atggaaatcc ataaattctt gcttggtcat
    18241 aggggcattg aggtaggccg cttctccctt atcataacgt gatttgaggt agaccttgct
    18301 catatcgata gtgttgacat cgataatagg cgctgccgca tcgtagaaat aaaaaccagc
    18361 accgtcatta agagcatgaa tcttttcagc caaggcatca cttgtcaaag gaccagtagc
    18421 gataaccgta ataacatctg tcggcaattc tgtaatttca tcacgaacca cttcaatcaa
    18481 ggggtggttg gcaacttttt cggtcaccat ttgagagaaa ccatcacggt ccactgcaag
    18541 ggcaccacct gcaggaacac gtgtagcctc agcagattcc aagataacag aacccaagcg
    18601 acgcatttct tccttgagaa gaccaactgc atttgtcaaa gcatccccac gcaaagaatt
    18661 ggaacaaacc aactcagcaa aattgtctgt tttatgctgg ggtgtagact tgacaccacg
    18721 catttcatat agtttaactg gaataccacg ctctgcgatt tggtaagctg cttcagaacc
    18781 tgccaaacca gcaccgataa cattgatata agattgagac acgacactaa tacctctttg
    18841 ggtgggacct aagtccatat taaaacaccc cactgggctg tttgacaccc acaaatcttt
    18901 ctaatttttt cttctactag tataacaaaa aaagggaaga aggcaaactt ccctgtttag
    18961 tcattttctt gatgtaaaga gatagtgagt attccagtta agaatcaatt atatgctact
    19021 ctataaaatc ttttccacat aacggatcga tagggactgt tattctatct cttgctataa
    19081 ccgtattatc taaaacagca taacattcaa catagtgatc tcctttaaac tgtgaatctt
    19141 cgtgatattt tattttacct gaaaaaatag caccacgctc acaatttctt ctaatagcct
    19201 cagcttcaat atttcttact ttccaataga cttcctgcga aacaaaatat ggtatagtag
    19261 ttctatgaat gatgaagcaa gtaaacaact aactgatgca cgatttaagc gtcttgttgg
    19321 tgttcagcgc acgacttttg aagagatatt agctgtatta aaaacagctt atcaacttaa
    19381 acacgcaaaa ggtggacgaa aacctaaatt aagcctagaa gaccttctta tggccactct
    19441 tcaatatgtg cgagaatatc gaacttatga acaaattgcg gctgattttg gtattcacga
    19501 aagcaactta atccgtcgga gccaatgggt ttaagtaact cttgttcaaa gtggtgttac
    19561 gatttcaaga actcctctca gttctgagga cacggtaatg attgatgcga cggaagtaaa
    19621 aatcaatcgc cctaaaaaaa caattagcga atcattctgg taaaaagaaa tttcacgcta
    19681 tgaaggctca agcgattgtc acaagtcaag ggagaattgt ttctttggat atcgctgtga
    19741 actattgtcg tgatatgaag ttgttcaaaa tgagtcgcag aaatatcgga caagctggta
    19801 aaatcttgtc tgacagtggt tatcaagggc tcatgaagat atatcctcaa gtacaaactc
    19861 cacgtaaatc cagcaaactc aagccgctaa cagctgaaga taaagcctgt aaccatgcgc
    19921 tatccaagga gagaagcaag attgagaaca tctttgccaa agtaaaaacg tttaaaatgt
    19981 tttcaacaat ctatcgaaat catcgtaaac gcttcggatt acgaatgaat ttgattgctg
    20041 gcattatcaa tcatgaacta ggattctagt tttgcaggaa gtctaatgta attctcaagc
    20101 tgtgagttca ttatgatttg taaatccgat atatcttcag cagctaaaaa cacattttga
    20161 aatgttccaa gtccgttttc accaaaacta tattctctct tactatactt actcttatta
    20221 ttctctatat agttagagtc aaggttctta acctcttgaa gttcattttc tttacttctg
    20281 aggttaaaat cttttacttc tgaagttaag ttttttgact tctgtaagtc attttctttt
    20341 acctgaaata tactcataaa gtctttaaca taaatgatgt tcggcttacc aagtccaagc
    20401 cttactcttt cgatcagtcc tattcctttt ttacatcaag ctcatctaat gtttttatgg
    20461 cagttggctt tgagatattt cttcttttca taatttcttc gacagtaaaa tagataaata
    20521 ctctgccttc cttgtctatc cagttattct taaatgacat tcctgtctgt ttaagaagta
    20581 tggaataaag gataattgct tctgctgata agccccgaaa ttcttctcta tcaactaata
    20641 tctccggtac ttttaagaag ttaaatcttt ccgcttctct gttataaaaa tattcaaagt
    20701 ccatgcaaga tcacctccct tcttaaaaat ttgcaaagaa aaagacgata gtttttcttc
    20761 tatcgccttt ggtaaccatt tttatgaaat tttttaaagt taaaaatata atttataata
    20821 ctatcttctt ccattttctg attttagttc tgaaagttcc aaaaggtgca accgtattta
    20881 catgaataaa cttatacact tcccaagtcg ctgttttagt cgcttcatca gcccactttc
    20941 tcatatgcgg ttcaaataat tcttcctcac tcaacgaatc aatcattgca gagatagaat
    21001 taatattttc atttaatttt gctttcaact cttgcagaga taaatgagcg taggtatctg
    21061 tgaaccactg atataattca ccaagttgat tccatttaaa tttatccgat ggtgttttta
    21121 cttgaagccc ctttctttca tcttcttccc atttaagaac caagttggtc caaccaacct
    21181 gataagcaag gttttctgct ggagttctgt caacttcatc agctctctta tcttttaaat
    21241 tttctggaat attatcaaat tctaaaatat atttctcaaa tgttttctct atctcagctt
    21301 ttagttcttc tttattttcg tatgttcgca aaaaatcacc ttctttctga tttatcaatc
    21361 ctttgattta aaatgacctt atttttctat ttataccttc taagaacgct ataactgctg
    21421 ctccaaccat cggtattccg tatgggctta ataaaaaact taataccaaa gcttctatac
    21481 caagtgatac atctttatat tatcatcact aattaagtta aacagaaaca gacagaatta
    21541 ttacatcctg tctcccttta ttgtttttct tcccagtcgt ggtaggcagc gtagagctga
    21601 cttttattcc actggtaaat cttagcgact tccttgatag cttggttttt cttcacacct
    21661 tgctggatgc gggtttgaat ttctacgaac aagtcttcct cgtccttttc ctccacaccc
    21721 tgactggcac cctcaacaat gagaagacat tcgcccttga gtggcgtttc agcaatgctt
    21781 tctaataact cagagatagt acctcgttgg tattcttcat agattttggt caattccctg
    21841 accaagacaa cggagcggtc accgtagact tctaacatat tttccaacgt gtctgctaca
    21901 cgatgaggtg attcataaaa aatctgtgtt tcaggataat cttttttcaa gccaaaaaat
    21961 tgcttctgct gacctgattt tctcggtaaa aaaccgtaaa agatatgtgg ctgtggcgct
    22021 aaaccactgg caatcaaggc agaaattcct gcagaggcac ctggaactgt cacaactgca
    22081 atttcttcct caatagctgc cttaactaaa tcatgaccag ggtctgaaat gctaggcaaa
    22141 ccggcatcag agacctgagc aatactttgc cctgctttca agaaaccaat caaatcagga
    22201 attttttcct tggcattgtg ctcatgaaaa ctgatctgct tggtggaaat gtcaaaatgc
    22261 ttgagcaaaa gccctgtatt gcgcgtatcc tcagcagcaa tccagtccac ttctttcaag
    22321 gtctggatag cacgaaaagt catatcatct agattgccaa tcggcgttgc cactagatac
    22381 agcttgccat agggagactg ccccttaaaa cttttttgaa tttgcatgct tactccctgt
    22441 ataacaactc atcacagaac atacattctt cgtcctgctc tcgacgttgt ccataaaaat
    22501 cattacatac gtgaaatcca tcacggtaaa tacgacggac actttcgcga acatgcttgg
    22561 ccttgacggg agcgtctgct tccacctcac ccaagcgttc tcgcaactta ctattttcca
    22621 agcgaagagc tgtattttct tctaccaagc tcttgagatt tttcttgatg gcttccacat
    22681 cggctaaggt taccaataat tgttgggaaa aatcatccag cgcgtcaaat aattcttttt
    22741 tgtccataaa acagcccttt cctttcttta tcattcattt ttgagtttat atttctttca
    22801 agaccagata ttccatggca ttttgaaagc tgacattagc ttgccacatt tttctagctt
    22861 ctagtaaatc ttgtagaatc actcttactc ttacctgcaa gaggtcctgc ccacagagaa
    22921 cttcaagaat ccgtaaaacc tgatcctgtt tttccttatc atctgccaag ttggctaatt
    22981 tggcaacctg tagataactt tcttttttct tagctactaa ccaagtcagc aggcgttcac
    23041 tttcatcgac caaggtccaa aaacttgcct gattagccaa cttttctgct tcagctcgcg
    23101 attgactaaa cttagctaaa agagtcgctt ttttcttaac aagtcccatt tgttctaaga
    23161 gtaagataag tttttcttct tgctttttaa agtggaagat ctgagtccga cttcggattg
    23221 tcggtaacat cttttcctca tcgctagtca agaagaaaat ataaacttca ctctggggtt
    23281 cttcgatgac cttgagcaga gaattggctg cgttgggatg cattttatcc gcttgctcga
    23341 taataaagac ctgttgctgg ctttcaatcc ctgcttgaga aaactgtccc accaattccc
    23401 gaatgcgttc tgtcttgatg acctgattga ctggcttaat caaggtgaca tctggaaact
    23461 cttcctgttc aatcagcttg caacttcggc atttctcaca tggtaagacg ccaactttat
    23521 ccgtacaaaa gaggctctta gctaaaaatt gcgccatttc caagcttcca aagaaacctg
    23581 aaaagagata ggcgtgattg agctggtctt gttctaagat acggacaaaa cggtcaaact
    23641 gagctggttg ccaagccttt agttgatctt gtttcatttg gccaagtcca ttccgtcaaa
    23701 caagacagcc ttggtagttt ccacaacttg ctccaaaggg agactagcat caatcttgac
    23761 aatgcgattt ccttcttttt ccaggagaga aaggtagcct tgacggactt tcttgtgcaa
    23821 ttccaaacct tccaagtcca gacgattgac ctcgcggtca ctattagcag caatgcgagc
    23881 cagcccttct tccacctcga tgtcaaaata gagtgtcaaa tcgggtttga ggccatctgt
    23941 cgcaaactga ttgagccagt caatggcttc aatatctaag ccacgaccaa atccctgata
    24001 ggcaacagaa ctatcgataa aacgatccat gatgaccaac ttgccagctt caagggctgg
    24061 aagaactttt tccaccaaat gctgtctgcg actggcaata tagagaagta gctctgtttt
    24121 agcatccatc tgagtatgac ttggatccaa aatcacttcc cgaatcttct ccccaatcaa
    24181 gactccgcca ggttcacggg tcgtcagcac ctttactcct ttttcctcta aaattggtag
    24241 cagagcctct aaaacactgg tcttgcctgc tccctctggt ccctcaagag agactaaaaa
    24301 tccttttgac atgtctaact catttctttt ttactacttc tattctatca aaaaaatagg
    24361 ggtttgtgac aatctttttg tcttctcatt tcatactcaa tgaaaatcaa agagcaaact
    24421 aggaagctag ccgcaggctg tacttgagta cggcaaggtg aagctgacgc agtttgaatt
    24481 tgattttcga agagtatcaa ccaccataaa agaggcagat aaaactacct cttatttacc
    24541 tagttcttgt gttcgtttgt aggcttgatg aactgcatcc atgactgttc ctcggaaagc
    24601 atgcgcttct aggcttgcta caccagcgat agtcgaaccg cctgggctac agacttggtc
    24661 tttcaatact ccaggatgtt gctgactttc aaggaccaat tgcccagctc ctaccacagt
    24721 ttgtgctgcc attttcaatg ctatttctcg tggtaatcct gtctgaacac ctgcatctgc
    24781 caaggcctcg ataaaaagat agacaaaggc tggtccacaa cctgcaagac ctgtcgctgc
    24841 atcgattaaa ctttctccta gttcaaccaa gagaccagcc ttggctaaaa gctgataaaa
    24901 gagctcactg tcctcagccc tgcaattagg agacaaggca taactaatca ctccttgccc
    24961 gatagaagca ggggtattag gcatcatacg aataattcgg tgttgacttg ggataagact
    25021 tgctagtttt tctaaggtca atccagctgc catcgaaatc aaaagaagac tttctctttt
    25081 ttcaaggatg gtctggtatt gagaaagcag ttcagaaaac tgagcaggct taactcctag
    25141 aaaaatcaca tctgcttctg caaacatttc ttcattgctg gaagcctgac caccaaagtc
    25201 tgcaatgaaa gcatctacct tagcttgact acgattggca aggagaatct catctgacgt
    25261 cctagtctgc aagacagatt ttgccaagct agcacccata ttccccaaac cgataaatcc
    25321 aatcttcatc tcttactccc ttatctgccc atcaccggca accacatact tgtagctggt
    25381 caactctttc aagcccatgg gaccacgcgc gtgcaatttc tgagtagaaa tccccatttc
    25441 acaaccaaga ccaaattgtc ctccatctgt gaaacgagtt gaggcattaa catacaccgc
    25501 tgcagagtcc acttgatctg taaagtatgc tgcagcttca gcattttccg tcacaatagc
    25561 atccgaatga tgggtgctgt gggattcaat gtgcgcaacc gcttcttcta aactgctcac
    25621 aaccttaaca gcaaggacat agtctaaaaa ctcggtgtca aagtcttggg tctcagctgc
    25681 ttgacctgaa acaaactggc ttgctttgct atccaagcgg aattgaattg gttccagtcc
    25741 agcttcctta cgctctgcaa ccagcatttg atccaagcga ggaaggatgc ttgctgcctt
    25801 gttttcatga accagcagaa cctccatggc attacaaaca gaaggacgac tggttttagc
    25861 attgttgatg atagacagcg ccttgtcttc gtctgcatcc ttatccacat agacatggac
    25921 aatcccagtc cctgtctcga taacaggtac aatcgcattc tcaaccactg cattgatcaa
    25981 gccagctcct ccacgaggaa tgagaaggtc tagatagccc ttggccttca tcatagcata
    26041 actactttca cggctagtat cctccaccag ttgaatcaca tttggatgaa tagtagtcgt
    26101 ctccaagccc ttcttcaagg ctgtgacaat ggcatgggtt gtttgatagg catccttacc
    26161 actacgaaga acaaccgcat ttccactctt aagagtcaaa gcagccgcat cagacgtcac
    26221 atttggacgg ctttcataga taataccgat gacacctata gctacacgtt tttttgtgat
    26281 aaccaaacca ttttcaagct gacttgtttc taaaacttca ccgattggat ctggtaaggc
    26341 aaccacttca cgaattcctc ttgccatcgc ttctatacga tctgcatcca aataaagacg
    26401 atccaacatc acatctgaga ttttcccctt agccgctgcc atatcgaggg cattagccgc
    26461 taaaatttcc tcagtagcag ccactaagtg atcagccatg gctagcaagg cttggttttt
    26521 cacttcttca ctagctgtgt taatcgattt tttaacagcc tgtacctgtt caaattgttc
    26581 ttgtctactc accatagttt acctctaaaa ttctgtaaaa agtagttgga tttcaggagt
    26641 aatggaaatc cagtcgtcac ggtaaatcaa gacacccttg gctttttgag aacgcaacat
    26701 atcctccaaa gcagatgctc caaattgcac gcgtcctttt ccaagtgatt ttccactttc
    26761 cttgtcaaat actgtcacga tatcaccgta agaaaaggct ccttctgctt caacgatacc
    26821 agataagaga agactctttc catgttgaga gagagcttcc gcagcccctt tatcaaccca
    26881 aatagaacct tgactctgag catagaaggc aagccattgt ttctgggtac gaagcccctt
    26941 ctcttgagca acaaagtaag aaccatcctc ggtctcctct gccgcctcaa tcatggaatc
    27001 tgatttcaag gatgagcaga tataaacagg aactcctgat tccgtcgcga tagttgcagc
    27061 cttgattttg gttaacatac ccccagttcc gtttgacgaa ccagctccac cagccatatc
    27121 aataatctca cgattgatgg tctcgattct ctccaagcgt ttggctcttg gatctgaatt
    27181 aggatttcca gtatagagac cgtccacatc tgtcaagaaa actaaaaggt ctgcttggac
    27241 catcgccgct acttgagcac ttagagtgtc attgtcccca accttgagct catcaataac
    27301 gacactatca ttctcattga tgataggaat tgccccacgg ttgagcaaaa ccgacaaagc
    27361 ctgatgggca tttttataac gacgcttatc cacaaagtca tcttgggtca gcaagatttg
    27421 tgcagaaacg atttgacgca agagaagatt ggtcgtatat tcttccaaca aaagtccctg
    27481 ccctaccgct gctgaagcct gtttatcagc aattttagtc ggacgctttt taaatcctaa
    27541 ggctccaaaa ccagccgcaa tggcacctga agacaccaaa atcaactcat gaccagcctc
    27601 gtgcagcata gccaactgct gggtaatatc ctttacctta ctacgtgata aacttccatc
    27661 ctcatttgtc agagaagaag tacccacctt aaagacaatc cgtttgtatt tcatagtctc
    27721 actccgatta ttcatattta tccattataa catgaataac aggaaataga aaagagttga
    27781 taggaaaaag aaggccctaa aaccttcttt tctactactg ttctgattcc gatttgttta
    27841 cactcgtttc agagctttgt gaagttgagg atgtcaaatc ttgactagtt gaaaaatttg
    27901 taacactttc atgttgactc gtttctttat tttcaagatc ctttttcact acttctttgg
    27961 attcttttat ttctgaatga cttgtagttt taattgtagc agatttctcg atcgaaacat
    28021 ccactaacca ggcaccactt gaatccacag tataaccttc tggtgtctta ccgttcacaa
    28081 gtaattgacc attttctttc aagaagtacc atttatcctt gtccttgatc caacccacag
    28141 ctcgtgaacc gtctgtcttg tagaaatacc aatcatttgc ctttttatgc cagccttgct
    28201 tcatagctcc tgaatcttcc agataataat gttgaccagc aacttctatc tcacctgtct
    28261 tcatgacacc agtagaatcc atatagtacc aggtttcttt atcttttacc cagcccattt
    28321 tcatttcccc tgatggagca aagtaatacc attgccccat atattgaagc catcctgtct
    28381 tcatagaacc atcaacatcc aaataatacc actgctcttt atcttgaatc caaccaattt
    28441 ccatctggtt ttctttgtta aaataatacc agattccagc gattttcttc caatttttag
    28501 cagaagcacc agagtctgtc aaatagaacc aatgattgtt ccattttttc cattgattct
    28561 gtaacaagat acccgtttgg ttaaagtaat accattcacc ttcgatttca ttccaaccga
    28621 cagcatactc tcctgtagaa tcaggcgctt gataccacca gttcccatat gcactcttat
    28681 gccaaccagc ttgaaaactt ggaatcggct tgtaggatgt tgaaatattg acaaaaccgt
    28741 cttttcgaat atcaaaaact gttgcatcat agtctttgct ggctgcgttg attctctcaa
    28801 ttcctcgttc tttgagccaa ttaacatact cactatcaac accatttttc caaggtagac
    28861 tatccgaagt ttgaacaatc aaactcggac tcaaattttt aatgaaatcc ttggtatttg
    28921 atttgttggt atcatggtga tgattaaact tcatcaaatc aacttttcca atgagaggac
    28981 catacttgtc ttctgctcca tgaacattat ctaaatcgcc cccaaggtaa attttcttgc
    29041 cattgacttt caccacgcta atcaaggaat tggaattgtc atcccaaatt ttctttaatt
    29101 cacccgatga atcagtttca ttttcataat tatagagctg aatatccatg tccccaaact
    29161 gaaaatgagc atccccttgt gtgatatttt gaataactga aacacctttt tctgcagcag
    29221 tctgtaaaac cttatcatag ccatacagat tatcccatag acgttcagaa ttagtaatac
    29281 gactatcact atatttctta agatagactc ggtcaactgg ataggtagac agtaattcat
    29341 caacatttcc aatatgatca ctgtgggtat gggtcaccaa aataaaatca agtttttgga
    29401 cacccaattc cttcaaacga cgaaagacac ggtctgttag aacatgctta taagacgttt
    29461 caattccttc tctccatgga tagcgagaat cacttccatc tgggaaatca taatcttctc
    29521 ctgtatccac catggcaaaa tgtccattgc tttcaagaat aatcgcatca ctgccacctt
    29581 cttgaacatt gataaagtgg attttatttc ctgaactttc ttgagcctga acattcccat
    29641 accatgacaa acctaaaaaa gcgcctacaa gtgctaaact agttaatttc tttttcattc
    29701 ttactttctc aatctctcca aggtttcctt aaaaatctct gggatatctg ctttaaattc
    29761 caaggtctta cctgttctcg gatgagtaaa acctaaagtc ttggcatgaa gaaattgtcc
    29821 atgtcctttc aaagtcttgc gaggaccata gacctcatca ccagcgactg gatggccgat
    29881 ataagccatg tggacacgga tttgatgagt gcgccctgtc tccagttgca actctactaa
    29941 gctataatcg ccaaagcgtt ccaagacgtg aaaacgcgtc actgcaggct tccctttagc
    30001 agttacagcc tgtttcttac ggtctttttc actccggcca atcggcgctt caattacacc
    30061 acgatcatta ggtagatttc catgaacaat cgcccaatat ttgcggagag actttttatc
    30121 cttgagttct tgggcaagtg ctagatgcgc atcatcgttt ttagcaatca tgagaagacc
    30181 tgacgtatcc ttatcaatac ggtgaacaat ccctggacgc agaaccccat tgatacccga
    30241 caagtcctta atatgataca tgagggcatt tactagggtt ccactggtat gaccagcact
    30301 cgggtgcaca accattccct gaggtttgtt aacgacagcc acatcctcat cttggtagac
    30361 tatttctagc ggaagatcct cagccacata ctctaatacc tctggttctg gcacatggta
    30421 agtgacgaca tcaccctctt ggactgtgta tttagctttc ttgacttgac cattgaccaa
    30481 gacctggcct gatttaattt gttcattcgc gagactacgt gataattctg acaaatctga
    30541 caaagcctta tccaaacgca gaccaccagt ttcaatttta atttccattt atttcctctt
    30601 ttagcattgc aatcaataaa ataatcactc caaccgtcag atagctatct gccacattga
    30661 aaattgcaaa gttgataaag tcaaggtgga acatatccac aacaaagccc tgactgaccc
    30721 tgtcaataaa gtttccaaga ccacccgcga ttattagagt caaacccaag accatccaga
    30781 atgagtcctc catgtgttta tgtaaatacc aaatggcacc tatcacgaca accagagtaa
    30841 tgacagcgaa taacaactgc tgatcttgta agatagaaaa ggctgcacct cgattttgca
    30901 ggtaggtcaa gctaacgaaa ttggggatcc aggagcgcac ttcacccagt ggaatctgct
    30961 ggacgatata ggatttgacc aactgatcca gcccaatcaa aagcagtaca atgactgcca
    31021 ctattgctct ttttttcatg atttcctctt ttgatcaaaa tattcttgca tgacttctac
    31081 gaagagagtc ccagcttgac taagctccac ttcttcacgt ttaacataga ccatgcggtt
    31141 atctaggtta tccttgagac gaataactgt aatgccatta acactgtcac tatctaaaaa
    31201 tccagaacct gtcgcatagg cgtccgtccg ctccaaaata ccattcaagg tggcacggtc
    31261 tgtcacatta aacatctgtg agctagcgct ggtatcaaca aagttctctg aataataaag
    31321 gtactcgtct ttctcttgag tgaaacgaac cgttggtaaa tccgctaaat cctccatgac
    31381 taattcctct ttctgggcta aaggatgacc ctcacagaga taaatatggg tatggaaagg
    31441 aatcaattcg atgacctcca gacctaactt ttcaacccgt tgcataatcc cctttttatt
    31501 ttgattgttg aggtagataa tcccaatctc actatgccct tgcgccactt catctaatat
    31561 ttgaacagta gttgattcaa aaatacggaa gttcttatag tcaggatagc gctctgaaaa
    31621 ggccgtaata gttggtggta agaagtcata gtgctggcta gcaacggaaa attcatcttt
    31681 ttcttcttca ggattggcat actgattttg aaaaatatca aatcctttaa ccaattcttg
    31741 cgctttttca taaaattcca tcccacgacg ggtcaagaaa gtccctgagc tggtccgacg
    31801 gaaaatctta aaacccaact ctttttccaa atcccgaaca gaaatagaca gactcggctg
    31861 actaacatac atcttttcag cagcttcacg aaaagtacca ctattggcaa tagccacaac
    31921 atagcgtaat tgttgaatgt tcatcttcta cccctaactt ctttatcttt tcattatacc
    31981 atattttaga agttttccaa aaaggaaaaa agaacatcct attcttctta actatcttca
    32041 ctatctgcct ttctcaagcc aaccttattt tcaaaatcaa ttcaaaaaaa taactggcta
    32101 cacaccacta caatatcgaa aagaaaaacg ttgactattt gtgaaaaaag aaaatgccgg
    32161 aaagttccga cattatttta gtaagctaac ttcctgaaaa taggagcaac cacacatccc
    32221 agaaggcagt gatattaatg agaatatgaa ctagtattgg atagtagaga tttttagtca
    32281 tgcgatacaa tagagctaaa ataagacctc cactagcata aatgaaaaaa gcaagaggag
    32341 ttaaagcaaa attgatatga atataaccga aaataatagc agaaagcaaa acatctccgt
    32401 accaaggcga gtttttgaaa aaggttgtca taatcacacc gcgataaatc aattcctcag
    32461 caataggggc gatgaagcaa acgatgagca agaaataagg gaactcctgt ctccccatca
    32521 tttctaccgt ttcattcaaa gaaatctgat ttgaagacag ggatatgaaa tacgaaaaga
    32581 ggaagtcaga catatatgaa atgatgtagc ccagtaaaag gtagatgaag tacctcagct
    32641 gccacttgaa atgaaaaatt ttctttcctg caaaaaccag tagatagatg accgctaaga
    32701 aaagaactcc gctctccatc aaaagcagaa tctgaaaaaa ttcacgactt gctggtagat
    32761 agggctttgc gaggtgattg aaaactctcc aaaaccaagt tgatttgtaa aaaattaagg
    32821 acaatgctag taaaatttga atagcccgtt ttttcatatt aaattctctg ctttctccac
    32881 ttgtttcttc atattactaa taaattttac ttaaaatctc gcagcacttc ctttgcaaag
    32941 atgattgcct cctccaatat atcctaaatc ataggtgcct ctgggacaaa atccatgaac
    33001 cggcagagtt aatactggtt ctatttccca atcgtaccta ccattaggat ttttaccata
    33061 tgaatctgct ccaccttgta tcagctctaa ttcttcattt gttagattta tattttttgt
    33121 cattaaaatc atgatatatt tcctccatta ttcttcagat tagttgagta atctgatact
    33181 catacctact tacaaaaaaa ctattatatt aagttggttt tttaatttgt ctagttgcaa
    33241 cattgacaaa catagtatag catatctttg cgaaatatcc tcttcaaatc atgaattgtc
    33301 atcaaaacat cttaaactat aaaatcaatt agtctcaagc tttctatcaa tttcttctcc
    33361 aaaatatgct ataataatac caaaagataa agaaggaaga cctatgatta aactactagc
    33421 cttggatatg gatggaaccc tcctcaatga agccaaggaa atcccacagg ctcacatcgc
    33481 tgctattcac caagccattg aaaaaggtgt caaactggtc ctctgtacgg gtcgccccct
    33541 tttcggtgtc ctcccctact acaaaaaact gggactcgac ctccagaacg aatatgtgat
    33601 tgttaacaac ggttgttcaa ctcaccagac tagcgactgg ggcttggttg actggcaaga
    33661 actgagtcca gctgacatcg aatacctcta tgaccttgct gaaaagagtg atgttcagtt
    33721 gacacttttt gacgagtcac attattttgt tctcggtggc aagcccaatc aagttattga
    33781 aaatgatgct aaactagtat tttcagacct gactgaaatt tctcttgagg aagcgactag
    33841 tggaaagttc cggatgttcc aaggtatgtt tttaggaaca aaagaacaaa cagacgattt
    33901 tgagcagcgt tttgctgaag agctttgcca acgattcagt ggagttcgtt cgcagcctgt
    33961 catttatgaa gcaatgccac ttggaacgac aaaggctact gctctttcac gactagctga
    34021 gattttgaag attgattcct cagagattat ggccatgggc gatgctaata acgatatcga
    34081 aatgctccag tttgcagggc ttgggattgc aatgggaaat gccagtgatt atgtcaaatc
    34141 tcttgcggat gccgttacct caagcaacga agaagacggc gttgcgcgtg ctattgagaa
    34201 atatattcta taaaaaagaa aagcaaatag acagaagtta ctgctatttg cttttttgct
    34261 aatattttaa aatacggact aatcaataga caagaatagc gaatcataaa aatcgattta
    34321 ctagatgcca tagtatctta tagttgctaa taagaagtta gtctagcaga atgaatctct
    34381 catttctccc acactaatct gtaatttgtc tatacatttc tggtcttcta tctcgaaaca
    34441 agccccagtt taggcgttca ctcgctccct tgtctaggtc ataagtagct aacagaacag
    34501 cttcttcttg tctttcagct cgttctagaa tagctcctgt ttcatccgtc ataaaggagg
    34561 aaccgtagaa gtcaagactg gaactctgtc cgccattttc ctcactagga gtaacctcct
    34621 ctaaaccata acgattggct gcgatgactg gaacaatatt cgctgctgcg tgcccttgca
    34681 tagtacgttg ccagtgacca caactatctg tatccaaaat cggctctgaa ccgatggctg
    34741 ttggataaaa gagcaattca gcaccattca atgcaagaca gcgcgctgtt tcagggaacc
    34801 attgatccca acagataccg ataccaatct tagcatagcg agtattccag accttgaaac
    34861 cagtgttacc aggcgtgaaa tagaattttt cttgataata atggtcatct ggtatgtggg
    34921 tctttcgata aacgcccagc acttccccat ctgcatcaat gacggcaata gagttataca
    34981 agacattacc atctttttca tagaaactga ttggtaaaac aacttgtagt tccttagcaa
    35041 tcaccttaaa atgctgaatg gcagtatttt ccgctacaga ttgggcatac tggtagtagt
    35101 catactgacg ttcctgacag aaatagggat gttcaaacaa ctcgggcaag agaataattt
    35161 gggctccttg ctcagcagcc tgacgtacta aacgctctgc ggtttggata tttgttgcca
    35221 catccttagc gcattgcatc tgaatggttg caactcttac atttctcatc tttttctcct
    35281 attctggaat ttgttgggtg atacagtgga tattgccacc acctaagaga atatctctgg
    35341 ctggtattcc gacaacttta cggtctgaga aacacttgct gaggatatct aaggccactt
    35401 ggtcgtttac atcctcaaac tgtggaacca agacagcctt gttggcgata taaaagttta
    35461 cgtaggaagc tgctagtcgt tcacctgcgt atcgcttttc ttctccttct tcatagatgt
    35521 agcctggcaa atcttcttct gtcacaactt gtcgaactgc agggataggc aatttatgaa
    35581 tggtgaagtg acaacctttt gcatctgttt cctgttctaa gagttcgaga tctgcttttg
    35641 acatggcata ctggggatca ttttcgtcat ctgtccaagc caaaacaagc tcagcaggac
    35701 caacaaaggc agcaacatta tcgacgtgtt cattggtttc atcctgataa ataccataag
    35761 gaagccaaat aactttttca gcaccaagac tttctaataa tgtgttttca atctcctctt
    35821 tagtcaggtt aggattgcga ccaggactaa gcaagcaact ttcagttacg agaatagttc
    35881 cttgaccatc gctatggatt gcgcctcctt ccagtacaaa aggtttagca tcatagacag
    35941 gcctttccaa ggcctcagca aaacgactgg ctacttggtc atcctcttca taatcttgat
    36001 aaagaccatc ataggtgcct ccccaagcat taaaggccca atccacggct aatttcttac
    36061 ctttatcatt gacgagaatg gttgggccag tatcacgcgc ccaggcatca ttggtgggaa
    36121 tgtctaaata aacaaccttg tctccaagat aggattgggc ttcagataga taggcctgct
    36181 ccaccaaaag atagactctt tccccttctg ctatggtctc gatgatctga gtaaatgctc
    36241 ttttagcagc ctttccttga aaaggccatg atcctggtcg agtcggccat atcatgaggg
    36301 taccatgatg gggttcgtac tctgctggca tgtgatagcc taattttttt ggactgtcca
    36361 tcatgataat ctccctttaa agtcttgata gccaaaagct ttgagtaagc tacagtctcc
    36421 ctgttcgtcc atgagataga gacttggcaa tccaatacca ttaaaggtat tatttttgac
    36481 aaaagaataa atggccatgt cttgaaaata aagtctgtct ccgatttgga ctggattttc
    36541 aaaactataa tcaccaatca catcgcccgt cagacaggta ttagaagaaa gtctgtaggt
    36601 atgggctttt tcctgtgact caaagccatt tctcaaaggt ggacgatagg gcatctcaag
    36661 tacatcaggc atatggcagg tcgcagaggc gtctaaaacc aagatttcca taccgttttc
    36721 tacaatatct aatacctcag ttgctaaata acccgcatta agcgcaatgg cttcaccagg
    36781 ctcgatatag atttcaagat tgtaagtttt tcggatacgc ttgatttctg aaatcagcaa
    36841 atccacatcg taaccttctc ttgtaatatg atgaccacca cccatattga gccattttac
    36901 ctcatgtaag tagggaccaa actgttcttc tactgctttc aaagttgttt gtaaatcatc
    36961 tgctccctgc tcgcaaaggg tatgaaaatg aagtccgtca accaaatcta gcaaatcact
    37021 cggaatcttg tctatagtaa ctccaaagcg agaacctggt gcacaagggt catagagcgc
    37081 gtgatctcct tgagttgaac actgagggtt gaggcgcaaa ccaacactga caccagcctc
    37141 tcgacaacgc ggaccgtgtt tacgcaactg tctctctgag ttaaagacga tatggtccat
    37201 tatctctagc aattcctcca agtctgcatc cttgaaagca ggcgcaaata catggacttc
    37261 accaggaaat tcttcccttg ccaatttggc ctcatagagt ccactagctg tcgtacctga
    37321 tagatactgg ctaatcaagg gataagtttt gtagagggaa tatgccttct gggcaagcaa
    37381 gaccttgcaa ccggcctctt cttgtacata ttgtagaatg cggcaattag cttctaactt
    37441 ggccaagtca ataacatagg ctggtgttgg tacttgttct aacttcatta gtccaccatt
    37501 tgtggatttt caaccacaac ccatggcaaa ccatactcat tcaaagcttc catgaatgga
    37561 tctggatcta actcctcaag gttatacact ccagcttgtt tccaagttcc gttcatgact
    37621 aattttgtcc caatcatggc tggaactcct gtcgtataag aaatagcttg cgaaccaacc
    37681 tctgcgtaac attcctgatg gtcgcagaca ttgtagatat agatagtctt tttgacaccg
    37741 tctttgacac ctgtaaagat acatccaata ttggtttttc cgactgtacg tggcccaaga
    37801 ctggcaggat ctggaagcaa ggctttcaaa aattgaattg gaacaatttc ttggccgtta
    37861 aagttaatgg tatccgtacg aaggagtcca acattttcaa gacatttcat gtgcgtcaag
    37921 taagattgac caaaagtcat aaagaaacga atgcgtttga cacctggaat gttcttggcc
    37981 aatgattcga tttcttcatg gtgaaggaga tacatatctt tttgtccaac ttgagggaaa
    38041 tcatactcac gcttgataga catagcttcg acttcgaccc atttcccatc ttcccagtaa
    38101 gaacctggcg cagaaacctc acggagatta atttctggat taaagttggt tgcaaatgga
    38161 taaccgtggt caccgccatt acagtctaaa atgtcgatat aatggatttc atcaaaatag
    38221 tgtttgaggg cataagctga aaagacacta gttacacctg ggtcaaaacc agaaccaaga
    38281 agagcagtca agcctgcttc tttgaatttc tcttgataag cccactgcca tgagtagtca
    38341 aagtaggctg taaaaccaag ttccttacaa cgtttttcgt agatagcacg ccactcaggg
    38401 tcttctgtgt cttctgcttc gtagttggct gtatcgatat agtgaacacc tgttgccaaa
    38461 caagcatcca taatggttaa atcttgataa ggcagagcta cattcaaaac agcttctggt
    38521 ttgtagcttt caatcagggc aatcacttct tcaaccttgt cagcatcaag tgctgcagtt
    38581 tcaattttag tacttgtttt gccttctagc ttcgctttca agtcatcgca ttttgacttg
    38641 gtacggctag caatcataat ctctgtaaat gtttcgctat cttgacaaat ctttgaaata
    38701 gcaacttggg caacgccccc acaaccaata actagtaaac gactcatttt tttccttcct
    38761 cttcttctaa aatgtcctca acatacttgg gcaacataaa ggctcccacg tgtaagtttg
    38821 cagtgtagta ttctgtgaaa agctggcgtt ttttccagcc ttccttgtca aaatctttga
    38881 cagggtggta ttttttcgat gcaaatccaa acaaccaata gccagctggg ctagttggaa
    38941 tatgggcctg ataaacccga ctgattggaa aggcttgatt gaccttgcgg tgcatgcttc
    39001 ggcaggccga ctcatcctcg tcaaagaagg gactcccatg ctggtaaatc atgatgccgt
    39061 cttccttcag agctcgataa ctattgccgt agaattcctt ggtaaagagt ccttccgtat
    39121 ggccaaatgg atctgtcgca tcgttgatga taatatcgta atcatcttcg cagtttcgca
    39181 aaaagcgtag cccattttgg tagtaaatgg taacacgagg atcatctagc cctgcagcaa
    39241 agtctgggaa atactcacga cagacctcga ccaacatctc atccggttcc acaatatcaa
    39301 tttgctccag ttcaggatag agggttaata cttgggcaac accgccgtca ccacccccaa
    39361 taaccaatac tttctttgga tttgggtgga cagccatggg aacgtgaacg gtcatttcat
    39421 tgtagacgaa atcatccgca tctgagaaca agacatggcc atttaaaatc agtattttcc
    39481 caaaagctgg cgtatccaag acttcgatat cctgccattc actttttcca gcgtaaagtt
    39541 gcttggctgt tctcagagac aatttgacat ctggagtatg aacttcagaa aaccataaat
    39601 ccatctattt ctccttatat tatattta
//
LOCUS       DAVDWT010000021        39140 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVDWT010000021 DAVDWT010000000
VERSION     DAVDWT010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39140
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.021"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(<1..1619)
                     /locus_tag="VUW50_001233"
     CDS             complement(<1..1619)
                     /locus_tag="VUW50_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HET7983128.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPVASQSKAEK
                     DYDAAMKKSEAAKKAYEEAKKKAEDAQKKYDEGQKKTEEKARKAEEASKEIAKATSEV
                     QNAYVKYQRVQRNSRLNEKERKKQLAEIDEEINKAKQILNEKNEDFKKVREEVIPEPT
                     ELAKDQRKAEEAKAEEKVAKRKYDYATLKVALAKSKVEAEEAELDNKAENLQNKVADL
                     EKEIANAEKTVADLEKEVAKLEKDVEDFKNSNGEQAEQYLAAAEKDLVAKKAELAEAK
                     IKAATKKAELEKAEAELENLLSTLDPEGKTQDELDKEAAEAELNKKVEALQNQVAELE
                     EELSKLEDNLKDAETNNVEDYIKEGLEEAIATKKAELEKTQKELDAALNELGPDGDEE
                     ETPAPAPQPEKPAPAPAPKPEQPAPAPKPEQPAPAPKPEKSADQQAEEDYARRSEEEY
                     NRLTQQQPPKAEKPAPAPAPKPEQPAPAPKTGWKQENGMWYFYNTDGSMATGWLQNNG
                     SWYYLNSNGAMATGWLQYNGSWYYLNANGAMA"
     gene            complement(2170..2409)
                     /locus_tag="VUW50_001234"
     CDS             complement(2170..2409)
                     /locus_tag="VUW50_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983129.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     GLHKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            complement(3545..4273)
                     /locus_tag="VUW50_001235"
     CDS             complement(3545..4273)
                     /locus_tag="VUW50_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983130.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFNPIISNTEYFLHIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLISFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IGEDFDIKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNHVLTLI
                     FLIKICADTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKEKEYELFKNSK"
     gene            complement(4270..4623)
                     /locus_tag="VUW50_001236"
     CDS             complement(4270..4623)
                     /locus_tag="VUW50_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET7983131.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKSACQGICVALAMSPGT
                     FIALTGAAVLTKKLINYIKVGGLGGWLIGAAAGVLATAAGKIAYCIGYGALNRGCDIS
                     GNPYPWDGFISATVR"
     gene            complement(4679..5581)
                     /locus_tag="VUW50_001237"
     CDS             complement(4679..5581)
                     /locus_tag="VUW50_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983132.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHLLVSNLQLNLEIPFDFVVSMSL
                     TFFVLILSKIILDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFVIIMLTTIFFWNIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTNTLKDKIQKSDLSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEMSISNNNNWSNKVDKIRIHLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            complement(5789..6163)
                     /locus_tag="VUW50_001238"
     CDS             complement(5789..6163)
                     /locus_tag="VUW50_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HET7983133.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLGRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            complement(6540..7517)
                     /locus_tag="VUW50_001239"
     CDS             complement(6540..7517)
                     /locus_tag="VUW50_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983134.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLISFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSIRR"
     gene            complement(7831..8889)
                     /locus_tag="VUW50_001240"
     CDS             complement(7831..8889)
                     /locus_tag="VUW50_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983135.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIGTVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREIALLLATVIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTVWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNFTPIILLAI
                     FENLLDSYLIQNQSKVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVVINEFIACLNSRCVPSQYDR
                     FFYKDRPYIFSRKSPIKG"
     gene            complement(9771..9983)
                     /locus_tag="VUW50_001241"
     CDS             complement(9771..9983)
                     /locus_tag="VUW50_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983136.1"
                     /translation="MMVVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKKLQTK"
     gene            complement(9991..10362)
                     /locus_tag="VUW50_001242"
     CDS             complement(9991..10362)
                     /locus_tag="VUW50_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
                     /protein_id="HET7983137.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WIFTNIIDGWDGRRDNQLRIG"
     gene            complement(10340..10678)
                     /locus_tag="VUW50_001243"
     CDS             complement(10340..10678)
                     /locus_tag="VUW50_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HET7983138.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            11144..12007
                     /locus_tag="VUW50_001244"
     CDS             11144..12007
                     /locus_tag="VUW50_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7983139.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYIRQHGAGELSDHAEQVVKKLWRKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVEIYYQAKER"
     gene            complement(12296..12766)
                     /locus_tag="VUW50_001245"
     CDS             complement(12296..12766)
                     /locus_tag="VUW50_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET7983140.1"
                     /translation="MRMPLKRPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVAVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVALQSHAYYEQKY
                     PLVSALSRPLISKKLVEIAHQTGATTIAHGCTGKGNDQVEYQIAVAKKANEAKK"
     gene            complement(12732..13538)
                     /locus_tag="VUW50_001246"
     CDS             complement(12732..13538)
                     /locus_tag="VUW50_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET7983141.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(13753..14394)
                     /locus_tag="VUW50_001247"
     CDS             complement(13753..14394)
                     /locus_tag="VUW50_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7983142.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            complement(14391..16499)
                     /locus_tag="VUW50_001248"
     CDS             complement(14391..16499)
                     /locus_tag="VUW50_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET7983143.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(16551..16838)
                     /locus_tag="VUW50_001249"
     CDS             complement(16551..16838)
                     /locus_tag="VUW50_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET7983144.1"
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFG
                     YSDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            17263..17583
                     /locus_tag="VUW50_001250"
     CDS             17263..17583
                     /locus_tag="VUW50_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HET7983145.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVPIGICTDNAKLIQKGFSLLELTEETSMLSHLKKEVETHYQ
                     PKKL"
     gene            complement(17636..18208)
                     /locus_tag="VUW50_001251"
     CDS             complement(17636..18208)
                     /locus_tag="VUW50_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET7983146.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            18450..19121
                     /gene="sdaAB"
                     /locus_tag="VUW50_001252"
     CDS             18450..19121
                     /gene="sdaAB"
                     /locus_tag="VUW50_001252"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HET7983147.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            19130..20002
                     /gene="sdaAA"
                     /locus_tag="VUW50_001253"
     CDS             19130..20002
                     /gene="sdaAA"
                     /locus_tag="VUW50_001253"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HET7983148.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            20024..20656
                     /locus_tag="VUW50_001254"
     CDS             20024..20656
                     /locus_tag="VUW50_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7983149.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            20706..20783
                     /locus_tag="VUW50_001255"
                     /pseudo
     CDS             20706..20783
                     /locus_tag="VUW50_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(21049..22899)
                     /locus_tag="VUW50_001256"
     CDS             complement(21049..22899)
                     /locus_tag="VUW50_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET7983150.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(22941..24098)
                     /locus_tag="VUW50_001257"
     CDS             complement(22941..24098)
                     /locus_tag="VUW50_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7983151.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIKWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYLNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKRTIIYLDKISQINKNML"
     gene            24156..25322
                     /locus_tag="VUW50_001258"
     CDS             24156..25322
                     /locus_tag="VUW50_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HET7983152.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TAMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            25461..25787
                     /locus_tag="VUW50_001259"
     CDS             25461..25787
                     /locus_tag="VUW50_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET7983153.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            25774..26367
                     /locus_tag="VUW50_001260"
     CDS             25774..26367
                     /locus_tag="VUW50_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HET7983154.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVTRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            26360..27271
                     /locus_tag="VUW50_001261"
     CDS             26360..27271
                     /locus_tag="VUW50_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HET7983155.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            27334..28398
                     /locus_tag="VUW50_001262"
     CDS             27334..28398
                     /locus_tag="VUW50_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983156.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADFDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIEAPEDSEARTGIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIVSILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(28576..29436)
                     /locus_tag="VUW50_001263"
     CDS             complement(28576..29436)
                     /locus_tag="VUW50_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HET7983157.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            complement(29711..30697)
                     /locus_tag="VUW50_001264"
     CDS             complement(29711..30697)
                     /locus_tag="VUW50_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HET7983158.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEDLWFKIDEEREQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(30969..32444)
                     /locus_tag="VUW50_001265"
     CDS             complement(30969..32444)
                     /locus_tag="VUW50_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HET7983159.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(32702..33625)
                     /locus_tag="VUW50_001266"
     CDS             complement(32702..33625)
                     /locus_tag="VUW50_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7983160.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(33639..34568)
                     /locus_tag="VUW50_001267"
     CDS             complement(33639..34568)
                     /locus_tag="VUW50_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7983161.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGTLWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(34887..35107)
                     /locus_tag="VUW50_001268"
                     /pseudo
     CDS             complement(34887..35107)
                     /locus_tag="VUW50_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35061..35321)
                     /locus_tag="VUW50_001269"
     CDS             complement(35061..35321)
                     /locus_tag="VUW50_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983162.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            complement(35748..35903)
                     /locus_tag="VUW50_001270"
                     /pseudo
     CDS             complement(35748..35903)
                     /locus_tag="VUW50_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(36088..36699)
                     /gene="rpsD"
                     /locus_tag="VUW50_001271"
     CDS             complement(36088..36699)
                     /gene="rpsD"
                     /locus_tag="VUW50_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET7983163.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(36894..37946)
                     /locus_tag="VUW50_001272"
     CDS             complement(36894..37946)
                     /locus_tag="VUW50_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET7983164.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(37943..38641)
                     /locus_tag="VUW50_001273"
     CDS             complement(37943..38641)
                     /locus_tag="VUW50_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7983165.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQI"
     gene            complement(38808..>39140)
                     /locus_tag="VUW50_001274"
     CDS             complement(38808..>39140)
                     /locus_tag="VUW50_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET7983166.1"
                     /translation="INVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMT
                     SSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLG
                     LKNKKEEN"
BASE COUNT        12098 a         8151 c         6597 g        12294 t
ORIGIN      
        1 gccatagcgc cgttagcgtt taggtaatac catgaaccgt tgtattggag ccaacctgta
       61 gccatagcgc cattgctgtt gaggtagtac catgaaccgt tgttttgtag ccaacctgta
      121 gccattgaac catcagtatt gtagaagtac cacataccgt tttcttgttt ccagcccgtt
      181 tttggtgcgg gagctggttg ctctggtttt ggtgctggag ctggagctgg tttttctgct
      241 tttggcggtt gctgttgagt caagcggtta tattcttctt ctgatctacg agcatagtct
      301 tcttcagctt gttgatctgc tgacttctct ggttttggtg caggagctgg ttgctctggt
      361 tttggtgcag gagctggttg ctctggtttt ggtgcaggag ctggagctgg tttttctggt
      421 tgaggagctg gcgctggagt ctcttcttca tctccatcag ggcctaactc attaagagct
      481 gcatctaatt ctttttgagt tttttccaat tcagcttttt tagtcgcgat agcttcttct
      541 aaaccttctt taatgtagtc ttcaacgttg tttgtttcag catctttaag attatcttca
      601 agttttgaaa gttcttcttc taattcagca acttggtttt gaagagcttc aactttttta
      661 ttcaactcag cttcagcagc ttctttatct aattcatcct gagtcttacc ttcagggtct
      721 agtgtagata ataggttttc aagttcagct tctgcttttt ctaattcagc ttttttagta
      781 gcagctttaa ttttagcttc tgctaattca gcttttttag caactaagtc tttttcagct
      841 gcagcaaggt attgttcagc ttgctcaccg tttgagtttt tgaaatcttc tacatctttt
      901 tcaagtttag caacttcttt ttctaaatct gctaccgttt tttcagcatt agctatttct
      961 ttttctaaat cagcaacttt attttgaaga ttctcagctt tgttatccaa ctcagcttct
     1021 tctgcttcta ctttactctt cgctagtgct acctttagag ttgcataatc atattttctc
     1081 ttagctactt tttcttctgc tttagcctct tctgcttttc tctgatcctt agctaactca
     1141 gttggttcag gaattacttc ttccctaact tttttaaaat cttcattctt ctcgttcaaa
     1201 atttgtttag ctttattgat ttcttcatca atttctgcta attgtttttt tcgttctttt
     1261 tcattcagtc tactattacg ttggactctc tgatacttta catacgcatt ttggacctca
     1321 gacgtggctt tagctatttc cttcgatgct tcttctgcct tacgagcttt ttcctcagtt
     1381 ttcttctgac cctcgtcata tttcttctga gcgtcttctg ctttcttttt agcttcttcg
     1441 taagccttct tagcagcttc agattttttc attgctgcat catagtcttt ctcagcttta
     1501 gactgactag ctacgggagc ttcttctgct cttacaaaag taggctgaga cgtaacaaaa
     1561 ccagccccta agatagcgac gctggctaga cttgttaaaa tcattttttt cttattcatc
     1621 taaatttacc tcttttctga tagtcatttt tagactatac ttatattaag tatactcccc
     1681 ctgtaaatat ttgtcaaatg ttttatatta catttttatt acaaatttct atatcataag
     1741 cttctactcc aaaaagataa agacactact tttgcccttt tcgattctct ttttaaacat
     1801 agcatcatct tccttcattc ctataaaaac atttccgatg agggagcttg tttttttaat
     1861 ttcgttaaac aacaaaaaac gcaagcttaa gcctgcggac aatctttata gccatttcat
     1921 ttctttcaca tctgttttac gaactttagc atatttctta gcgcttgtaa aatgtagagt
     1981 aactggttta aatactccgc ttttgtctct tcgtaaacct aaaactgcgg atcttttatt
     2041 tttatcctca aaaaatatga cgtccaattt tatagtattt ttagataaat ccttagctac
     2101 gatgcaacaa cttgttagct tagatataga agatatcgcc gataaactca cataaagata
     2161 tcctttcgat tacatcattg tacataggat atttctaaaa tcttctaaaa taaaaacacc
     2221 agcttgaata gcaggttttg tttgactagc ataatctcgt gtgattttgt gtaagccaaa
     2281 taaatgatgt aaatcagtca agcaaaaatt cactttgaaa atctctaaat acttataatt
     2341 cgttgatact tgacatgttt ttccattgaa tctttcttga taatccaaaa tacaatcaag
     2401 taatttcaac gacaaactcc atattttctc aaaataaaaa gcacggggca gttctcacta
     2461 ctgcccaaag tggttgatta gagtcggctt ctctaactcc ctatgttgtg cttattaggc
     2521 ttatcgcaag ggaacagttt ttagctctgc caagccacat agtgtgctta ctactatgtg
     2581 tccatgtcac catgaattgc tgaacggttt aacgagaaag tatagtagat tgaaacaaga
     2641 tgtgaacaaa tcgattagga aagtcaaatt aatttctaga aatattttaa cagtcataac
     2701 ggactagccc agattcaatc tactatatta tttttaggtt tatcccaact caattatgac
     2761 atcttttttt caaaagtcaa tatatctcac tcttttcaac gacaagaaag aggctgataa
     2821 tcgaccaacc tctgttttta gatacagttc aagtccaaac tattctttat agaaaagtcc
     2881 gataaattcg agcaatagtt aacatactta caaatacaac tcccaaaata gataacacta
     2941 ttttcacaaa actcagttcc gaacctatga catatatata tgaaaaagag gggctagtcc
     3001 tacacatata tacaataagt agccaacaag ttttatctca ccaattcctg caaaaaaatg
     3061 tttcatgtaa cgattctcca tttactccat taaatcagtt ttattttaga ctatcataaa
     3121 acaatcacct cttcaataaa aaccattggc gaaatactat tctgaagcat tgataggtca
     3181 gatttctcag aatagagctg tcctaactat cgagcagctc aaatacatat atatgagcaa
     3241 ttctaagcct agagttgcta taaaataaat gttttcgggc tattctaagt ctttgactgc
     3301 ttaaaatttt acattctgaa ggattctaaa gctagaacag ctcaaaatca cgagaactaa
     3361 ggtattcgaa acttcagagt aatacaatcc ccataggaat tctccattct tattaagcat
     3421 gattaaaatg taatcctaac aagtgatcct cgattgtgtt tgtagcactt agttacctag
     3481 ataagactat ttccctttat catatctgat tttaagccgt tctaagattt aacctatgtt
     3541 tctattactt gctattttta aataattcgt attccttttc tttgataaat ggaactgaaa
     3601 aattagaaaa aagaagtaaa accgaaaaca aatagcctgc tttttgaatc actaaaagat
     3661 atcctaaaaa taagtaagct ataacatcca atgcgaaaag aaaaaatatt ctcctcttca
     3721 ttaaagtact tgtgtcaaat aatctgatga ataaaaattt tatcgtggta tctgcacaaa
     3781 tttttatcaa aaagataaga gtcagtacat gattatcaac tgtaccataa agaatagatg
     3841 gaaatatagt taatataaaa ctagaaatct ctaaatattt tatccaagga gattgagaca
     3901 ccttgcttct aaactgacta attttcttat attcgttagt caatttaata tcaaaatctt
     3961 ccccaattaa atgaaagatt gctaactcta ggataaaaac acttattaat acggtataag
     4021 tcaaaaaact ttctgggaaa aatttattga ttaagatggg gaaactgatt aacagaatcg
     4081 aatttatggc tagacagata gatactattt tatttttcac agaatataga ggtagtgata
     4141 ctattgagta gataaaagct aaaaaaatat gcaaaaaata ctccgtgtta cttatgatcg
     4201 gattaaatga taaaaatata tacacccaac acacgatgat atgtattttt cgaatcttat
     4261 ctacattact catcttactg tcgcagatat gaatccatcc caaggataag ggttcccgct
     4321 aatatcacaa cctctattaa gagcaccata tccaatacag taagctattt ttcctgctgc
     4381 tgtagccaat acacctgctg ctgcaccaat aagccagcct ccaagacctc caaccttaat
     4441 atagtttatt agttttttgg ttaaaactgc agctccagtc aatgctataa aagttcctgg
     4501 actcatagct aaagctacgc aaattccttg acaagcactc ttgcttaaat aagctccccc
     4561 atcaagatac atcatttctt cttcatcaat cacaacataa ttatttggta atacaagttc
     4621 catttgtttt ctcctcctta gttgaaattg tttcatttgt tttacaaata ggacgaactt
     4681 aaactatctt tttattactc agtaataatt tccgagattt cattttatga tactctctat
     4741 tcatacagaa atcaaaattt tgccttaacc aacttgtata atagaaaaaa tctaattgat
     4801 tgattctgat gaggtgtatc ctaattttat caaccttgtt ggaccagttg ttattgttac
     4861 taatagacat ttcatacagc agtaactcga caagacctgt acacagaagg gtgttatgag
     4921 aatcttcgat ttttttcaaa atatcctcaa aaagttcact atccgttaaa aaggtggatt
     4981 ggttcatcgc atcatagaga agaagagaca ttaagtcaga ataactgaga tctgattttt
     5041 gtattttatc ctttaacgta tttgtcaaat cactttcaaa cgaatcttct ttttcccatt
     5101 tattttgatt gaaaagaaat tccgtcttat taaaaacgag atgattgata tccattatat
     5161 tccaaaaaaa tattgtagta agcataatga tgacaaaagg aatttttaaa ataaatacta
     5221 ggataatagc aatttcaaga ataaaactcc atataatcct agatcttaaa tcatcaggcg
     5281 acaaactttt tcgatggaat gaaaaatagg gcgagaacct cacatttact ttcttcctgc
     5341 ctataaaaat aaaaggaaaa atcgtaataa gagcttcttt cttgtacaaa tctttcaatg
     5401 cgtatagtat gtctaaaatt atttttgata atatcaaaac aaaaaaagta agactcatac
     5461 taaccacaaa atcaaaggga atttctagat tcaattgcaa gttactcact aacaaatgca
     5521 actctagcca caagacaaaa agagggagca ttaataaaaa aatgattagt ttacgtatca
     5581 taattttaca gtgtccttat cattttctta attttggata aattatcatt gctatgatta
     5641 aaatcaaaaa aacgacattc atccaaataa tcaatttttt tatttcttcg ctaacaaaat
     5701 cataggctaa aaaagttatg gatgatataa atagacatac aaaaaaaata ataacaaaaa
     5761 ttttttccat tttaataccc atatctagtt accacaaggc gtttaaattt gggtctctcc
     5821 tatttacatc attgttggcc cagattgttg taatagcatc accaattgac atccaagctg
     5881 caaatccatt aatagcagac agtgcaaatc ctgccactct atttgcaact cgtaccccaa
     5941 catatcttgc agctgctcca gcaattgcgc ttgtaactcg ccccaaacca taattacgaa
     6001 tataatttct aactagactg attgctcctc cacctaaaac accattaaaa gctatattaa
     6061 tggctgttgc aacaggccat ctcggaatag aaaatccccc atcaagatac atcatctctt
     6121 cttgctcaag agcaacataa ttatttggta atacgagttc catttatttt ctcctttttt
     6181 attaagcata gttaggatag tttcctaaca agtgatagac gactgtgttt ttatcactta
     6241 gctacctaga taagatatcc tttctctacc ttatttgttt ttataggtta cctaaggttt
     6301 aaaatcaatt attgtttgaa acttaacagt tctaaaccac tagcaataaa tgattgcgaa
     6361 ctcactaata ctatttcaaa atttattttt tttgacttag taacgcaatt ccaattagca
     6421 atacacctat tataaataaa agtatttccc aaaatttatt atccgtgtta atgagaggta
     6481 taagatacat actgactaat atgcaacttg cccctataat ggcaaatatt attctggttt
     6541 tatctcctaa ttgaaacatg tttgatcccc atatacactt caagcaaaag ttggatttct
     6601 gaaatggcta aagataaaga taatggttgc gagctattta aactatcatc aatattctgc
     6661 tcgattaaaa caaagtctgg tcttacctct aaaattttct ttaacattga ttcatattta
     6721 tttttttcat tataatgccg taaatataga tttaatttta taacatccaa cataccataa
     6781 agtgatattg gatgatttat ttttttaaat cgcttaactt ctaataaatc aatttgttca
     6841 cgcacgctct ctgaaagata tggcttgatc aaactgatat aagataaagc cacgcatggc
     6901 aattcagatt ttgattgcaa tttccaaatt tcatgataat cctgaagatt aaactcacaa
     6961 ttcttcttag attccagtat tgttagtacc tttgatttta acagaaataa ttgttgattc
     7021 tcaattctat aaagtttttg tactaatgct atgataatag aaaatgaaat aagaggcaaa
     7081 agcaacggtt tgtcatacac aaaagtaaag aaaaaatagt atgaaattag tgttgtaaag
     7141 tacaagaagt agtaaaattt aaacagattc ttagttgttt gactctcctt ataaaacact
     7201 gctattcctg gactaaatcc cattagttgg tgaaagagaa ttcgatagta tttcctagct
     7261 cctataatga tcacaaatat aaaataaaaa taaattggtc ttaatttaca aaagattgag
     7321 gaaactagta atagcaaagt atgtaagcat tgagacaaaa tatagaataa cggtatcgcc
     7381 aatacaaagg ttaagatggc atgagaggga gttcctaaga tgtccaattt tataaatact
     7441 gggctgacac aaaatatcgt gcttataagc agtaaaatta ttaaaattgt gtcaaaaaaa
     7501 taacgcttat ttttcattag tctactttcc attgttaaat aatactactg ctaacaccaa
     7561 gccaacttag cacattgcat cacctaatcc tgcgctactt gtccataatc ctttaatgtt
     7621 tctactaaaa ttattccaat ctcccccatc aagatagctt atttatccta gacaacgcct
     7681 actgcaacag tataaataag attcatcagc catcttttct ggcaatcata tgggtagact
     7741 aatgatattt ctagttactc aaaaatatct agctatacta gagttatttc taatccctag
     7801 gacacttgtt aatccaaatg taacaaattt tcacccctta ataggactct ttctggaaaa
     7861 aatataaggt ctatctttat aaaaaaatct atcatattgg gatggcacgc atctggaatt
     7921 taaacaagct ataaattcat ttataactac tatttgttta gaggcaacag catcttgtat
     7981 accgccctcg aaagaaccat cttgtaattc cgaaatattt ttgtttaaat attgttttga
     8041 caaatctctt aatgcttcat tatcgtaaat gattcccaaa aatccaacct tatatagtaa
     8101 ataatttaaa cttacaaagt aattgaatcc catagtatat ttttctttat aaagcaaagg
     8161 taaaacttta taaaaaatat ctaagtcaac cttactctga ttctgaatca agtaggagtc
     8221 aagcaaattt tcaaaaatag ctaacaatat gattggtgta aagtttttgt attcacatgt
     8281 caagtattct gagtatcgtg cagatgatat ttcttttata taactttttg aaagcaagat
     8341 tttttcaaat tcatcatcta ttattaatct tctatttcca atccaaacgg tattaccacc
     8401 caaataagat tgtgcaaata gcaatatgta agaaaatatg aacgtaaata ctgaaagagg
     8461 tagaattcta tgcgtaaaaa aatagacgat aacggtagcc agaagtaaag ctatctctcg
     8521 tgacaatcga attcttttca gcatcttttt taacagtccg taaggttgtc cttggcaata
     8581 ttcttgaact aaattaatcg gaatgacatc tctcaccatt tctggaaagt agagtaagtt
     8641 tataggttga aatctccagc tcccatcata agtaaatgga tatagaacca aatacttaaa
     8701 agattttttt cttataaact taattatcac aatatgaatg actgttccaa tgaagtatat
     8761 taaatataaa agtaaaaccg ccatcaatat acttacaaaa atccccttgg actcaatcaa
     8821 tggaaaataa aatattgcaa ctagaagcga ataaagtccc gctaataaat atttctgatc
     8881 atctttcatt caaaacacca tattatttac taagaataca tcaatacaat agagcaactc
     8941 tgtaagtcaa tatttgataa agcaaagctt taaccccttc cccagacgtt agcttttctt
     9001 caacccacta cagttgacaa ggagcccaaa ttattgataa aaactttagt catcatcgtc
     9061 tctataaaaa tattttctat acactcttga taataccaaa attaatgcca atatcatacc
     9121 aagagctata ataatagaat ttattagatt tattttagta tatccaatca ctagtccaat
     9181 aattggacta gcagacatgc ttagatataa taaaaataac aaaaaatgaa tttcaccaat
     9241 tcctgcaaaa aaatgtttca tagctatagt tttacgccca taaataccca tcaaatttac
     9301 cgtctacgat atctaaacca tatgcaatgg ctcctcctaa tgagaagact tcagcaacag
     9361 tagccgcaat attaagagca ttagtaatag ttgccatact aactccacca atatatcttg
     9421 ttatagcact tagttaccta gataagatat cctttagctt gcttccaaaa tttaagtctg
     9481 cacctataat atgagttcat attagtactg ctaaatccta tctatagtaa tgtcaaacac
     9541 ctaaataaag tcatctctcc aattcattca agaaatgaag aaaggcaaaa aatctttaaa
     9601 ataagaaaaa tgcatattat caggtgttca aaaatattga tgatatacat tatatagata
     9661 gaaagattta ctcaatctca ttttcaaatt gagttttttc ccagcctcgt ttttaattta
     9721 tatcttcaat agaatctaat gaccgagcta ctgtattaca cagattaata ttatttagtc
     9781 tgtaattttt ttaaaaaact taatagtaaa tttgctccac tgattgctat caatatttca
     9841 attggtaaaa aatagtaatt ccaaataaaa tgagaaatca gcaatgatag aaaaataaca
     9901 atatcaattt tcctgttgtt gacatactct gtaattttat gatacaggaa tgcaacaata
     9961 actgaaatta aaacaaccat cataatacta ttatcctatt ctcaattgat tatcacgtcg
    10021 cccatcccag ccatcaataa tattggtaaa aatccaatca gctccagcac caattccagc
    10081 agatgcaata cttctaataa cagtatttgc aaaagcattt ccaatatgaa ttcctaatac
    10141 tcctaaacct ttaacaatcc cagctaccag tgcggagcgt aatccataag atgcaagaac
    10201 tagttgaagg cctccacctc ctgctgctgc taatgctgca tatgctgcac cagtaatggc
    10261 tcctgtaatt gcccacctgg gtatatatat agccccccca tcaaggtaca tcatctcttc
    10321 ttcatcaatc acaacataat tatttggtaa tacgagttcc atttattttc tcctttatac
    10381 tatgtaatac cacaaaatga ttttgaccta aatcattctt aacttgaaga atcgcaggaa
    10441 atgttaattt tggagtcaaa tcttccagtg ctactcgaac agcttttact gtaaaattca
    10501 acttattcag acctgaaaca ataccactga cagtcgttcc atacatgtct gtaccaataa
    10561 tttccctaat cttcatgatt gatagttcct ttttgtaaga taaaagaact gttgaaacaa
    10621 ctgccgcagc acaatctgac tggtcatgtt gcatcgtaca aggatatttt tttaacatag
    10681 aaatactcaa ttctttagaa ttaatctaac ttttctttga tgtcacccaa acctcaatca
    10741 ctgcccagta gttcttgaat tagtttattt tcaaccgtat cattctttgt tcttgatatc
    10801 tttccaagca ttgtagtaca gcagattttt gcccaaggaa tgagcctcct ttaacaactc
    10861 aagctccctt acattatatc ggccagcccc atggaaagat tatagccaga ctaatcaatg
    10921 atataataca tactagaata caaaatattt ttttattcat actttttctc ctatctttat
    10981 tttctaccta catagtacct cttattaagc tcatttcaaa attacgttaa cttttttcac
    11041 aaatttattt cgactttctt ttttactttt tacattgcta tgatacaata tttttaacaa
    11101 aattttattg tttgttaact tttttcacga aaaggagaaa atcatgcgtt atgatttcgg
    11161 aaaggtctat aaagaaatac gtgagtcaaa ggggctgacc caagaagagg tctgtggggg
    11221 tgttctctca agaaccagct tatcaaaaat cgaaagtggc aagacaaccc caaaatatga
    11281 aaatatggaa tttcttctcc gtcaaatcaa tatgagtttt gaagagtttg aatatatctg
    11341 tcaactttat caaccaagtc aacgcacaga gattatgcaa acctatctca atatgcgttc
    11401 cattatcggg accagcgatc ttgtcaattt atttcaaaaa tgccaagact acctcaagac
    11461 ccaccacgat ctccctatag aagaaatcag agatatgctt gaagttgtca tttatatccg
    11521 tcaacacggg gcaggggaac tatcagatca cgcagaacag gttgtcaaaa aactttggag
    11581 aaaaattgaa aaacaagata catggtatga gagtgatcta aaaatcctca ataccattct
    11641 tttcagtttt cccattgaat acctccatct catcactgga aaaatcttgc aacgcttaga
    11701 agtctataaa aactatcaac atttatatga cttgcgaata gcaatcctgc taaacctttc
    11761 cacactctat ctatacaatc aagataaaaa catgtgtaag caaatctgct acactttact
    11821 agaggacgcc aagaataaga aaagctacga taggctggct atctgctatg tccgtattgg
    11881 gatttgtaca gacgattcta aacttatcca aaaagggttc tcccttctgg agctaaccga
    11941 ggaaacttct atgctgtctc atctcaaaaa agaagtagag atctattacc aagcgaagga
    12001 aagataaaaa agccgaggga tttcctcgac tctttacata ttatttgttc gttagttctt
    12061 aataccagcc attgttaaac cagaagtttt tagctctgcc aagccacata gtgtgtttac
    12121 tactatgtgg ccatgtcacc atgaatcgcc gaacgattta acgagaaagt atgactttta
    12181 cgtttatccc aactcaatta tgacattttt ttcaaaagtc aatatatctc actttttcaa
    12241 cgacaagaaa gaggctgcca attgaccaac ctcttattct gaacccatca ctccatcact
    12301 ttttagcttc attcgctttc ttagcgactg caatctggta ttcgacttgg tcattcccct
    12361 tacctgtaca accatgagca attgtagtcg ctcctgtctg atgcgctatt tcaaccaatt
    12421 ttttagaaat aagagggcgg ctcaaggcag ataccaaggg gtacttttgt tcatagtagg
    12481 catgtgactg gagagctact agcacataat ctgtagcaaa ttcgtcctta acatcaatga
    12541 cataagattc gactgcccca accttaagag ccttatcatg gatgaaatct aagtctttcc
    12601 cttcacccac atccatacaa actgcaacaa catcatagtc tttcttcagc caggtaatag
    12661 caactgatgt gtctaaaccg ccagaatagg ccaaaattac tttttcttta ctcatgttct
    12721 tttccttctt tctatttttc aatggacgct ttaaaggcat cctcaatgag tttgtctaat
    12781 tccccagact ccttcaactt ttgaatggtt ttatcgactg cctctttcaa ttccttgcta
    12841 tcttttttca tggctaccgc ataggaatca tcttgctctt tttcaaaatt gaggtctgcg
    12901 attgctaaat caggattatt ttccacaaat cccttggcaa cgggttcttc aaaaataacg
    12961 gcatccactt gccctgattt taaatctgtg attaaattcc catttttagg cagagatacg
    13021 agggaagaat tttgtagcaa atctttcgcc atcgtctctt gaatcgaacc tttctgcgct
    13081 ccgacctttt tctgcgccaa atcgttgaca gactgataag tggctaaatc agattttttg
    13141 acaatgagtt tattttttgc agtatagtag ggagttgaaa aatcaaacac cttgctccgt
    13201 tcatctgtct tagaaacacc tgatatggca aggtcagctt ttcctgattg aacgctagcc
    13261 agtacattgt caaaactcat aggagatagt tccaattcga cacctagttc tgttgcgata
    13321 gccttggcta attcgatatc tgaacccaca atctgatttt tcccatcaac cactttttga
    13381 tattcaaatg gagcaaaatc tggattgagg gctacaacca attttccttt ggacttgatg
    13441 gcttcaattc cagccgattg attgttacta caagcaaata gtagggggag catcactaaa
    13501 ccaaacatcg tcatcaacac cttcttcatc ttattcataa caaggcctcc tttatgttta
    13561 ttctttttta ttgtataaat aatactccta tactcatata ctcatccttt gtcaagccta
    13621 aattgaaaaa attttcaatt tgaaacaaaa aaaacctaga aatgctacat aaaatgtcat
    13681 tcctaggcgt atttatgcta tttttctctg gaaaacatga gtattcagcc agtcaaaagg
    13741 agctgaatga actcattttc cctcgcccaa ttcaatgaca cgatgacatt gttgggctac
    13801 ataagcatcg tgggtcacaa taatgactgt tttcccttct cgattcatct ctaagagaaa
    13861 cttcaagacc aaatctctat tttcaggatc cagcgaacct gtaggttcat cggctaaaat
    13921 cagctggctg ggttttaaga tggctctagc aactgcaatt cgttgttgtt cgcccccaga
    13981 caactcggag accctttgat gcaaagtagc tgacaaacct actctctcta aaatctcttc
    14041 cacctttttg agcttgtctt tcttaggcaa tttcacatat ttcagcgcca gcatgagatt
    14101 gtactcgacc gtttcatcat caatcagggc aaaattttga aacagataag agatatgttc
    14161 acggattatt gtttgcgact tagcagaatt aaccgctaga tttgtctgac caaaaatctc
    14221 ataccgtccg ctataatcac catctatcaa acccaataaa tttaacaagg tcgacttccc
    14281 actaccactc ttaccaacaa tagctaccaa atccccctga tcaatcctga gagataagtt
    14341 atccaaaatc acttttcccc caatggtttt ggtaatattt ttcaactcaa tcataagatg
    14401 ccccctttca ataactctac tagacttctt ttctccatcc tagaagctaa gcctagcaca
    14461 aatagtatat ccagacatgt aaaacctgca aacagtagaa gtggtaagaa cgcatgggca
    14521 aagaaaatca agactagaag agggaaacta tagcccagca agagcagaac gaggagagga
    14581 cggtagcgat cgaccagttt ccaccccata aacttcttgg taatgatatc cctgcgcttc
    14641 aataagaaag ttgttactag taagaagtag gaaatcatca tgctaaggag accaaacaaa
    14701 gcaaagagta ggttaaaatt ccgaacagca tctctataag aatccacttt ctcttgttga
    14761 atggcttgaa tagatgaaaa ttttaaataa tttccatctg acaatttctc aactaactct
    14821 gtaatctctt tttgatgttg aaccgtattt tcaattttaa tcggattatt taagccagtt
    14881 gttgacaggg aggctttctc atcccacatc atatcagaat cattgaccaa gctaataatt
    14941 ggattggaga gattttcctt tcgcttatca ctatatggga aaaatgacca atctccttca
    15001 taataggcaa tctcgacatc catctcctct atcgttcgtt tttgctgctc ttcatacttc
    15061 atcgaatgaa aggcaattaa cttccccaag agctgatttt tatcttcttc acctttcgta
    15121 cttgctggca tcaaaataac ttttttaata ccggtatctg gtagcttgaa tcccttgctc
    15181 tttagaaaat tgcgattggc atagtaaaca tccaccgtat ctgttaactg atattgctga
    15241 atctgttctg attggacaaa attttttaca ggaagactgc tactctgcac atagcccgcc
    15301 tgcgtttttt ctaccaaatc ctgataaaat cgatagaaat aatctgtaga tttccctgac
    15361 cctgctagct cttcttgcca cagattatca ttgagtttga aggtttctaa ggtcaggtaa
    15421 ttaccttgac ttacccactg ttgctgataa gcaagttctt tgttttcttg ttctaaactt
    15481 ctgcccaccc caatcagtaa ggccgtcagt aaaatagttg tccctatttt catcacataa
    15541 ttgaagataa gaccaaattt gaaagatgaa aaacctttca gcagagagct aattgtcatt
    15601 ttttggatta agaggtaagt caaccaactg ataaagagat aaagctgcaa cagcaaaaaa
    15661 tgagacaacc acagcatagg aaacaaatct tttggcttat aatcaagcaa gaaaaacacg
    15721 cctagattga tcacaagagc cccacctagg aggaggtaaa ggttgccttt tacaacatca
    15781 gctaaaacag ccctatcttg aaaaccaagt aatttttgta ccccaactct tttcatctcc
    15841 atcatcggtt gatacactgt cactaacaca agaagcaaaa tagccaagac aaaaacaatg
    15901 gcagataaaa gcaaatctcg atttatgact tccactgcac ttttgtaggt cggctctagc
    15961 aaggtagcct ggtctatctt gaaaaaatcg ctccatttct gtacaatcct atccttgtcc
    16021 atctcttgtg tagaagttat cgtatagcga ccatttaaac tacgagatgt atccttgata
    16081 taggtttgaa aagtcataag ctgaataggt ttggctttta gaaaggtcgg aatcgtacca
    16141 agtttattgg aaatttcttt attactatag actccttcac catctgtggt aaaatcaaga
    16201 gaagaaatcc caaactcttg gtaggggaag gtatctttat caaaaacacc agacttgacc
    16261 acctcatcac cactgtctgt tttgatgatg gagactttat actcctttga tacatcctca
    16321 aaaaatcgaa gaacagacgc tgcaggttcg ttaatatctt tcaaatacaa atccaaagaa
    16381 tctacagtct ttcccatttc ttgaatccga accacttctc gtgtatagtt tacattaaaa
    16441 acaaaaaaag tggtacacag aagcaggagt aacatacaga aattactgat ttttttcata
    16501 aaagtgcctc cactacagtt ttccctctcc aaataggaga gggaagaatt ttactcaaat
    16561 ccatagaaat attccattcc tgttggcgga atcttggtga gggaagctct agcccatgcc
    16621 tcaggttttg cataatcctt agaggttcta cccccatgtc taacagttgc tgtatgatat
    16681 cgagtagaat gtaagtaatc agaatatcca aaagttcctg tccaacctac tccatagttc
    16741 cattgaccac cctcaaccca tactgctaaa gcatttgtag tagcaagtat accacatacc
    16801 attaaagatg caacaccaat tttaactaat tttctcatga tttatttctc ctattatcga
    16861 gagctgttcc gtaaaaacag ctatagtaat ttatcaacaa gtatcgtagt ccattggact
    16921 caccttcctt ctatctcatt tcatttttaa acaacgaact tactcttaac cgggagaatg
    16981 gtgcgcttct gtatcaggtc cttgaacaac cagagtagta ttaagcacac tctaactatt
    17041 actcaagaat cagtccaata aacaatatgg atagcgcttt caaattttta aactcctatc
    17101 ccatcctttt atctatataa taagcgaaaa tataataact gtcaagtaac tgaagtgaat
    17161 tttataaaaa aattacaagc caaatttgta aagtttacac taagccgcta gggcaatcgt
    17221 ctatcagaat gtccgtttat ttgtcaataa tccgagaaaa tcttgcaacg cttagaagtc
    17281 tataaaaact atcaacattt atatgacttg cgaatagcaa tcctgctaaa cctttccaca
    17341 ctctatctat acaatcaaga taaaaacatg tgtaagcaaa tctgctacac tttactggag
    17401 gacgccaaga ataagaaaag ctacgatagg cttgctatct gctatgtccc tattgggatt
    17461 tgtacagata atgctaaact tatccaaaaa ggtttctcac ttctggagct gaccgaggaa
    17521 acttctatgc tgtctcatct caaaaaagaa gtagagaccc attatcaacc aaagaaatta
    17581 taaaaaaagt cgagggagct cctcgacctt ttacatatta tttgttcgtt agttcttaat
    17641 accagccatt gttaagccag aagtttttag cagcagtcca tgaaccgtaa cgtcctgcaa
    17701 cgtaggcatc tgctacacgt tcttggtttt cagctgagta gtcaccgttc aggtatgaat
    17761 ctgttaattg gtaacgtccg atataacgtc catttgtagc tgtatagcta ccacctgatt
    17821 ctttttgagc gatccattct ttggcttctg cttcagatcc gcttacagtt gaaacaactg
    17881 tttcacttac tactggagct gaaacagttt catcttgagc ggctggtgcc gcataagtag
    17941 ctggcgctgg tgttgcaaca ggcgctacag ggccatcgat aaccaactct tgatcaacat
    18001 aaatcaaatg aatgttatca atgtggttgt tttctgccaa tttttcaact gttgtgttgt
    18061 gagtttcagc gatttctgaa agtgtatcac cttctttaac agtgtaagtt gatgattctt
    18121 gagcagatac aaatgatgga gcaaatactg caaacaaggc agctactcct gcaagagttg
    18181 ctttaatctt tttagttatt gatttcatat tcgaaaattc tccttctttc tatagttcta
    18241 tcataccctt taaatattac cgtttcttga cacttttatg tagaaatatt acaaaattat
    18301 tttttatttc cctaaataag gtgttttttg tcataaaaga tactattttt atgctatttg
    18361 aggtattaga aaggttttca tccacaatta aagccaagcc cctcattctt tgatataata
    18421 gagataagaa tttttataag gggaaactga tgaaatcact tcgttttcaa tctgtctttg
    18481 atatcatcgg accagttatg attggcccat ctagtagcca taccgctggt gctgttcgta
    18541 ttgggaagat tgtctcttcc atttttgatg atactccgac agaagttgaa ttccaacttt
    18601 ttaactcatt tgccaagacc tatcgtggtc acggaacaga cctagccctt gttgcaggta
    18661 ttttaggaat ggatacagat gatcctgaaa ttccaaatag tctggaaatt gcccacaagc
    18721 gtggtatcaa gattgtctgg accattcaga aagacagcaa tgcccctcac cctaacacca
    18781 ctaaaattac tgttaaaaat gcccacaaga ccatcagcgt gactggtatt tctatcggtg
    18841 gaggaaatat tcaggtaacc gaactcaatg gttttgccgt ctctctcaat atgaatacac
    18901 cgactatcat catcgttcat caagatattc caggtatgat tgcccttgtt acagaggcgc
    18961 tttcccgcta tggtatcaat atcgcccaga tgaatgtcac tcgtgaaaaa gctggtgaaa
    19021 aagccattat gattatcgaa gttgacagtc gcaactgtga tgaggctatc gaagaaattc
    19081 gaaaaatccc tcatctccac aatgtcaatt tctttaaata ggaggaagca tgttttattc
    19141 tatcaaagaa ttggtcgagc aagcagatct agactttcaa ggaaatgtcg cagaactcat
    19201 gattacaaca gagtttgaat tgaccggtcg cgaacgtgaa gaagtcttcc ttctcatgga
    19261 acgcaatctg gaagttatga aagcctctgt ccaacttggc ctcaatgaaa ataaatctcg
    19321 tagtggcctg acaggtggag atgctgccaa attggatcac tacatcgaaa acggaaaaac
    19381 tctgtcagat tacacgattc tctctgctgc ccgaaatgcc atcgcagtca atgaacacaa
    19441 tgccaaaatg ggcttggtct gtgccactcc aaccgctgga agtgctggct gtctgccatc
    19501 cgttctcact gctgctattg aaaaattaga cctcagccac gagcaacaat tggatttcct
    19561 ctttgctgct ggtgcctttg gactagtcat cgcaaacaat gcctccatct caggtgctga
    19621 gggtgggtgt caagctgaag ttggttcagc ctctgctatg agtgctgccg ccttgactct
    19681 ggctgcaggt ggaacacctt atcaggccag tcaagctatt gcctttgtca ttaaaaatat
    19741 gctaggcctc atctgtgacc ctgttgcagg tttggtcgaa gttccctgtg tcaaacgtaa
    19801 tgccatggga gctagctttg ctttcatcgc agcagacatg gccttggcag gtatcgaatc
    19861 taaaatccct gtggatgaag tgatcgatgc catgtaccaa gtaggagcaa gcatgccaac
    19921 tgcctttcgt gaaacagctg aaggtggact cgctaccacc cctactggtc gtcgcctcca
    19981 aaaagaaatt ttcggagaat aagcttatca aataggagaa accatgacct ctatcacagc
    20041 gatttttttc gatttggatg gaaccctcgt tgatagttct atcgggattc acaatgcctt
    20101 tacctatacc tttaaagagc tgggggtgcc tagccctgat gccaaaacta ttcgtggttt
    20161 tatgggacca cctctcgaaa gtagttttgc gacctgcctg tccaaagacc aaatttctga
    20221 agccgtgcag atatatcgtt cttactataa ggcaaaaggc atctatgaag ctcaactctt
    20281 tcctcagatt atagacttgc ttgaggagtt atcgagcagt tatccactct acatcaccac
    20341 gacaaaggat acatctactg ctcaagacat ggcaaaaaac ttggaaatcc atcatttctt
    20401 tgatggcatt tatggttcta gccctgaagc accccataag gcagatgtca ttcaccaagc
    20461 cttgcagaca catcaactag caccagaaca agccatcatc atcggagata ccaagtttga
    20521 tatgctggga gctcgagaaa caggtattca gaaattggcc atcacttggg gatttggaga
    20581 gcaagcagat ctactaaact atcaacctga ttatatcgct cacaaaccct tagaagtttt
    20641 ggcgtatttt caatagcata gactgggtaa caattccatt tcagaagaaa atgaagtaat
    20701 actcaatgaa aatcaaagag caaactagga aactagctgc gggctgctca aaacactgtt
    20761 ttgaggttgc agatagaact gacgaagtca gctcaaaaca ctgttttaag gttgtggatg
    20821 gaactgacga agtcagctca aaacactgtt ttgaggttgt ggatagaact tacgaagtca
    20881 gctcaaaaca ctgttttgag gttgtggata gaactgacga agtcagtaac catatacggc
    20941 aaggcgacgt tgacgtggtt tgaagagatt ttcgaagagt ataagtctct acataataaa
    21001 acgcatatta ccaaggttgt gtaacttgat actatgcgtt tttctttttt attcaatgtg
    21061 ggttgtttga ttagcaaagg cgataatagc ttgcttcaac tcatctccac tgagagtgcg
    21121 gaactctcca atcttttgat tgatggattc taaaggcatg acattaacct taccaacgaa
    21181 aatcttatcc ataacttgat tatcaacgag ttcggttgat accaagagtt cttcgtagag
    21241 tttttcacct gggcggattc caacttcaac gattggaatt tcactttcag tgtggccact
    21301 tagaagcacc atcttcttgg ccaagtcata aatcttgact ggtttgccca tatcaaggat
    21361 aaagacttcc ccatctttgg cataagcacc agcatggata accagacggc tagcttctgg
    21421 aatggtcata aaataacggg tcatgcggaa gtctgtcacc gttacaggcc caccttcagc
    21481 aatctgacgt tcaaagactg gaatgacact accacggcta ccaagaacat tcccaaaacg
    21541 aactgcacag taggttgatt ggctacgttg gttaaagcca gtgacaatca actccgccac
    21601 gcgcttggtt gctcccataa catttggtgg attgactgcc ttatctgtcg aaatcataac
    21661 catcttagac actttagctt catcaacagc cttagcaaca ttgtaagttc cacggatatt
    21721 gtttttgaag gcttcttttg gattgcgctc catcatagga acatgcttgt gggctgccgc
    21781 atgataaaca atagcaggtt tgtactgctc aaagacttgc aacaaacgat catagtcttg
    21841 aatgtccgca atcacaggta cataatcaat cccttggaac ttacgaatca attcatgata
    21901 aacaaggtag attgagtttt ccccatgacc gagcaagaca atgcgttcag gattgaagcg
    21961 actaacttga cgacagattt cagaaccgat tgaacctcca gctcctgtga ctaagatggt
    22021 cttacctgtc agttctgcac ccagacgcga ttcgtcaaga cggatttcct gacgacccaa
    22081 aaggtccgta atatcaattt tttggaagcc agtacctgct tggtgaaggc cctgaacaac
    22141 agtttcaacc ttaggcatct tgtaacattt gacacccagc ttattacaca tctgcaagat
    22201 acgctcatat tctgacggat ccagcgacgg aatcgcaacg atgacacgct cgatttgatg
    22261 gcgtttggct aattcaggca gattgtcata agagcccaaa acaggaatac caccaagttt
    22321 ttgacccttt ttcttagaat ccttatccaa aataccgacc agttctaatt cactggttgg
    22381 atgttggtaa ctatccataa aaagagcccc accatcaccg gcaccaatca agaaggtccg
    22441 acggtgttct ccatcaccac tacctttttt gcgtctggag tagattaact gccaagtaat
    22501 ccgtggcaat aaaatcaaga aggtactcaa caagataaag agaatgatga aacggatgga
    22561 gaagagtggc aagaaggcat aacagatact atatgacaag acactgctag cagtcacacc
    22621 aaaaaagatt ttcatgaaat ccgtaatctt gctgtaacga ctaatgctcg cgttcaaccc
    22681 ccaaaaacca atcatcaatt gatagaacag gaaggccaaa ctcgtataga taatgtagtc
    22741 aacaggtgct ggattaatca gcccataaaa taaaatataa gatacaatga tggaaaccac
    22801 catactgaaa atatcaaata ttccccagaa aacctgcttt tgttgtttat ttaaaatttc
    22861 caccagatca atcacataat ctgttagttt tttattcata gaaatctact cctccttaaa
    22921 agagttttac tatattgtta ttatagcata tttttgttta tttggcttat tttatccaaa
    22981 taaattattg ttctcttgta ctccttcttc catttaaatc ctctaattaa acctacaatc
    23041 gtcccaaagc cataagcaaa gtgaatggaa aataaaagaa agggcatcac aattagaaat
    23101 ccatttttat gttttaataa agtcagcaaa gtgagtagtg acaaaagtag aaaataggca
    23161 cctaatagta aagttatgaa tacgaatgtg atcggtaaca atgctagact aaacacaaga
    23221 ctcaaaacaa ataaacaagg aacatagtga aataatgata aacacttaaa ctgaacatga
    23281 cttgtcaagc caatccacaa accatttaaa tacttttgat gcagcatttt cttgaatgtt
    23341 ggtcgaatat actgataaga tagaatactt gggctatagc ggattttata accatattct
    23401 cgaattctat aatgaatatc attatcttca gttcggccaa gttgctcatt tactaaacca
    23461 accttctgga aaacctctcg tttatacatt ccatgaaaaa tagaagaaac atatctatcc
    23521 tcagaactat ttcgataatt ggcaatgcta ctgccaaaca tattttcctc aacaagatgc
    23581 aaggtctctg cccattttcc ttttccttcg acaatcgtcg gtctaggccc cccacagaca
    23641 aattcacctt gttgaataat agccacattg ttcattacaa aactctcagt aacttttgaa
    23701 tgagcatcaa tttttaaaat aaggtcccct acagaatgtt taactcccag gttaaaacca
    23761 ctagcttgat ttttcttagg attgttatac aatctaattg agttaaactc tgtatcttcc
    23821 tttataaatt gctgaatgat agctgtggtc ccatctgtgg acatagcatt tataaataga
    23881 atttcaatat tctctttagg ataagtttga ttttttaagt cttcaattag accaggcaga
    23941 tatttttctt cattgtatgc actgattaca atggatacaa gaagactttc aacattttga
    24001 gtcactacta ctctcctgaa tttttccttc caccatttta tcataatttt caagactttc
    24061 ccatttttat cttgaaagcc agaaacctta cttgacatta tagtgaaaaa gccttaaaat
    24121 aagctgttat taaaatcagc tagaagaggt attatatgaa aaagcaatca ctcttttttg
    24181 ttccaggtat tatcctgatt ggtgtttcct tgcgaactcc ttttactgtt ttacccatta
    24241 ttttgggaaa tatttcgcaa ggtctggagg tagaagttag ttcgcttggt gtcttgacca
    24301 gcctgcctct ccttatgttt accctctttt caccattttc tacccaactg gctcagaaaa
    24361 tcggcttgga gcatctcttc acctacagcc tcttcttctt gaccatcggc tcacttattc
    24421 gactaatcaa tctgccccta ctctatctag gaaccttgat ggttggggca agtgtcgcag
    24481 tcatcaatgt cctgcttcct agtcttatcc aagccaatca accaaagaaa attggttttc
    24541 tgaccacctt atatgtaacg tctatgggga ttgcaacggc tctggcttcc tatctagctg
    24601 tgcccattac acaagccagt tcttggaaag gacttatcct tctcctcacg ttactctgtc
    24661 tagcaacttt tttggtctgg ctcccaaatc accgctataa tcatagacta gctccacaaa
    24721 ccaaacaaaa aagtcaaata aaggtcatgc gtaataaaca ggtttgggca attattatct
    24781 tttcaggttt tcaatccttg atcttttaca ccgccatgac ctggttacct accatgtcta
    24841 tccatgcagg tctatccagt cacgaagctg gcttgctgac ttctatctta tctctgatta
    24901 gcattccttt ttcaatgacc atcccaagcc tgacaaccag tttatctact cgcaaccgtc
    24961 agctcatgct cactctggtt tcactagctg gtgtggtcgg catttccatg ctctttttcc
    25021 caatcaataa tttcatttac tggcttgcca tccatctcct catcggaacc gcaaccagcg
    25081 ccctcttccc ttatctcatg gtcaactttt cactcaagac aagcgcccct gaaaagacag
    25141 cccaattgtc cggcctatct caaacaggag gctatatcct agcagccttt gggccaaccc
    25201 tctttggtta cagttttgac ctgttccact cttgggtacc atctgtagct gccctcttgc
    25261 tcatcgatat cctgatgact gtggccctct ttacagtgga cagagcggat aagatccttt
    25321 aaacttctag ttttagctag aagtttttta tatataaaaa ttttacacat ttctcttgac
    25381 agggctttct tttagatgta caatgtatgt gtagaaaaat tatatataaa aatcttacac
    25441 attagaaaag gaggtttccc atgtactttc caacatcctc tgccttgatt gaatttctca
    25501 tcttggccgt actggagcag ggtgattctt atggttatga gattagccaa accattaagc
    25561 tcatcgctaa tatcaaagaa tccacactct atcccattct caaaaaattg gaaggcaata
    25621 gctttctgac aacctattct agagagttcc aaggtcgcat gcgcaaatac tactccttga
    25681 caaacggtgg tatagagcag ctcttgaccc taaaagatga atggacactc tatacagaca
    25741 ccatcaatgg catcatagaa gggagtatcc gccatgacaa gaactgaata cctgactcag
    25801 ctagaactct atctcaagaa actacctgaa gctgaccgta tcgaagccat ggactatttc
    25861 agagagctct ttgacgatgc tggagtcgaa ggagaagaag aactcatcgc tagtttggga
    25921 actcccaaag aagcggccca cgaagttcta tccaatcttc tcgataaaaa aatcaatgaa
    25981 gcacccgctc aaaaaaataa ccgacaaatt ttacatatcg ccttgttagc cctccttgca
    26041 gcacctatcg gcattcctct gggaatcgcc atcctcgtga ccctgttcgc aatccttgta
    26101 gccgctttga ctgtcattct ggctttcttt gcagtttcca tactgggtat catcggcgga
    26161 ttcctatttt tagttgaaag tttcactgtc ctcgcccaag ccaaatcagc ctttatcttg
    26221 atttttggtt ctggtttact ggctatcggt gcttcttcgc tagttttact tggcatttcc
    26281 tatgtaactc gcttcttcgg tctactcatt gttcgtctgg tacaatttgt tcttaaaaaa
    26341 ggaaagagag gtaaccagca tgcgtaaatg gacaaaagga tttctcatct ttggtgtggt
    26401 gactaccgtt atcggcttta tcctgctttt tgtaggtatc caatctgacg ggattaagag
    26461 cctactttcc atgtccaaag aacctgtcta tgatagccgt acggaaaagc taacctttgg
    26521 caaggaagtc gaaaacctag aaattactct ccaccaacac acgctcacca tcacagactc
    26581 tttcgatgat caaatccaca tttcttacca tccatctctt tctgctcacc atgatcttat
    26641 caccaatcag aacgatagaa ctctgagtct cactgataag aaactgtctg aaactccgtt
    26701 tctctcttct ggaattggtg ggattcttca tatcgcaagt agctactcta gtcgttttga
    26761 agaagttatt ctccgactac caaaagggag aactctaaaa gggatcaaca tctcagccaa
    26821 tcgcggacaa accaccatca taaatgctag ccttgaaaat gcgaccctca atacaaacag
    26881 ctatatcctc cgaattgaag gaagtcgtat caaaaacagt aaactcacaa cacccaatat
    26941 cgttaatatc tttgatacag ttcttacaga tagtcagcta gagtcaacag agaatcactt
    27001 ccacgctgaa aatatccaag tccatggtaa ggttgaactg actgccaaag attatctcag
    27061 aatcatccta gaccagaaag aaagccaacg aattaactgg gacatctcaa gcaactatgg
    27121 ttctatcttc caattcacaa gagaaaagcc tgaatcaaga ggtacggaat taagcaaccc
    27181 ttacaaaact gaaaaaaccg atgtcaagga tcaactcatt gcgagatctg atgataatat
    27241 tgatctaata tccacaccaa gcagacgttg acaaaaacgc ttacatctgc tactatggaa
    27301 gcatatttat ctaactaaaa aggaggttct accatgaaac aagaatggtt tgaaagtaat
    27361 gattttgtaa aaacaacaag caagaacaag cctgaagagc aagctcaaga ggttgcagac
    27421 aaggctgaag aaacgatagc cgatttcgat acaccaattg aaaaaaatac tcagttagag
    27481 gaggaagtct ctcaagctga agtcgaattg gaaagccagc aagaagagaa aattgaagct
    27541 cctgaagaca gtgaagcgag aacaggaata gaagaaaaga aggcatctaa ttctactgaa
    27601 gaagagccag acctttctaa agaaacagaa aaagtcacta tagctgaaga gagccaagaa
    27661 gctcttcctc agcaaaaagc aaccacgaaa gagccacttc ttatcagtaa atctttagaa
    27721 agtccttata tccccgacca agctccaaaa tctagggata aatggaaaga gcaagtgctt
    27781 gatttttggt cttggctagt ggaagcgatc aaatctccta caagtaagtt ggaaacaagt
    27841 atcacacaca gttacacagc ctttctcttg ctcattctgt tttctgcatc ttcctttttc
    27901 tttagtatct atcacatcaa acatgcttac tatggacata tagcaagcat taacagtcgc
    27961 ttccctgagc agctagctcc tttaactctt ttttctatcg tctctatcct agtagcgaca
    28021 acactcttct tcttttcatt cctcttgggt agtttcgttg tgagacgatt tatccaccag
    28081 gaaaaggact ggacgctaga caaggttctc caacaatata gtcaactctt ggcaattcca
    28141 atcttcctca ctgctattgc tagtttcttt gctttctttg atagcctacg atttacagcc
    28201 ctcttgtgtg tgattagcat tggaatcatt ctgcttgcca gtctccatat cattacaaga
    28261 ccaagtcaag caagtgaaac cgactccttc tatcaattat tcttgtctgt ccttgtgaac
    28321 ggagttatta tcctcctctt ctttgtagct gaagtggcac tgattggaga ttatcttcgt
    28381 atcttggcct ttctttaaac ttcatactct tcaaaaatca aattcaaacc acgtcagctt
    28441 cgccttaccg tactcaagta cagcctgcgg ctagcttcct agtttgctct ttgattttca
    28501 ttgagtagca atcctgtcat ccttggcagg attttttcta tgtcaaaaaa gcaagtcgat
    28561 tgacctgctt ctgctttact tttcttgtgc taatgtttta aaatctttgt ctaatatggg
    28621 ttgcacttct atttgattgg cgtctagttg gtggtaaaaa gatgggggta atacctctaa
    28681 gaattttgca tagtccaagc gagcaatggc ttcatagtct tctggtttag agccatctcc
    28741 tgtgatttga accaagtcaa tctcccactg gacttcctta tccaccaatt gctcaatata
    28801 ggctgcagga acaaacggtt ctctcggtaa aactgtcttg actccttgag ctagatggta
    28861 aacgccatgc acttttcctt ctccatcctg atagaaggaa actctcgcaa agtaggcatc
    28921 tgtctcttga accgcacgca cacgcgcttc aaactgagcc aagccatctg cctctaaaaa
    28981 gcttttcaag ttctcacgac taaagaaaat aggattaaaa attccttggc aaatcgtaaa
    29041 acgagctaca gggtctgcta gataggcttg aatacttgct tggaaataag cttcaaaatc
    29101 aaaaccatct agcccttcaa tatcagtccc tgtataagca agcaaagcat ctaaaaatga
    29161 cttgataatc tgccagtctg tctttgttag taggtcagga agatcgacac ggtaagcctt
    29221 ttgaccatca gcataactga ccttaaagag aaattgtgat tgccccacta tcgcacactc
    29281 gatatattcg agacgattaa gaggctgacg gagatagact acatcatagc tatgtgactc
    29341 caaaccgtct agcaaggcca agatcgactt ggcattcaaa atctcttgtt ctcctaaaat
    29401 gctctgttta tttggaataa aaaatgtttt cgccataact ggactccttt gaaatcgttt
    29461 acatacagta taccttattt ttctgaaaga tacaaaaaca aactttatac tcttcgaaaa
    29521 tctcttcaaa ccgcgtcagc ttcgccttgc cgtagatata tgtaactgac ttcgtcagtc
    29581 ttatctacaa cctcaaagca gtgctttgag cagcctgcag ctagcttcct agtttgctct
    29641 ttgattttca ttgagtatta gatttttgag tacaaagcat agaaaaacag cccctaagga
    29701 ctgtttgatc ttatacagta gctgcctgat ccaagctttc accgatagcg gctaggcgct
    29761 caatcacttc agcttgtgtc aattcatttt ttgaaacata gcggttacgt gggtgaacac
    29821 ggcactcgtg tgagcatcca cgaaggtact tgtcttcatt ttcttctgat gtcaagatac
    29881 gacggttaca gaagggattt ccacagttga cataacgttc acatggtgtt ccatcaaacc
    29941 agtctttccc tacgatagtt gggttgacat ggttgacatc aacggcaata cgctcgtcaa
    30001 agacgtacat tttcccatcc caaagctcac cttgaacttc tgggtcttta ccgtaagttg
    30061 cgattcctcc gtgcaattgg ccgacatctt tgtagccttc acggaccatc cagcctgaga
    30121 atttctcaca gcgaacgcca cctgtacagt aaaccacgac acgcttgtcc atgaattttt
    30181 ccttgttatc acggacccat tgtggtaact cacggaagtt gcgaatatct gggcgaatag
    30241 ccccacggaa atgtcctagg tcgtactcat aatcgttacg tgtgtcaagg acaacggtat
    30301 ctttatcaag aagcgcttct ttgaactctt ttggagacaa gtaagcacct gttgtttcaa
    30361 gtgggttgat gtcattgtca aagtcgttgt cttccaaacc aaggtggaca atttctttct
    30421 tgtagcgaac aaacatcttc ttgaaggctt gttctctttc ttcgtcaatc ttgaaccaga
    30481 gatcttccat acctgggagg ctgtgaacgt agtccatgta tttttgagtt gtttcatagt
    30541 cacctgaaac tgttccgtta attccctcgt cagcgactag gatacggcct ttaaggccga
    30601 ttgatttaca gaaagccaag tggtctgcag caaattgctc tgcattttca attggagtat
    30661 aaaggtagta aagtaagaca cgaatatctt ttgccataag atttgttctc ttttctattc
    30721 ttaaattttc taaatttttt atttatccat actagtatac ctgcttgaga aaacgaatgc
    30781 aatttatttg actggaaatt gtagagggtt ctcgaaattt cctgaatggt taaaataagg
    30841 acaagagaaa acatggatat ctatatcctt gtgccaaaaa aaccactgcc ctccccagac
    30901 caacctgagg aaagcagtga ttcttatttt aggagttagg aatgaataca cgaaatcaat
    30961 ttagctgatt attttttgtt tttcaagaat tcatcgtatt gtttttgcat ttcgttcaat
    31021 actttttcgt aggcaccttc agatttcaat ttttccatca attctggaat agctttatct
    31081 gggtctacag taccagtgtt gatagctgta tcaaattgtt gcattgtgtt agcaatagct
    31141 gagatttcag atttcacatt gtcagtattg aagataaatc caagcgctgg agattcttta
    31201 gcttctgcca attctttctt agaattttcg atttgttggt ctgtaacgtt ttcgttgatg
    31261 taaaggatcc agttgttacc agtgttccat ccacccatgt gagtgtttcc tttgtagcca
    31321 tcaagaacgc gaacacggtt ttctttacct tcaatttttt cccagttctt gccttctgga
    31381 ccgtaaacaa gaccgttcaa gagttctgga ttcgtattca agaggttcaa gatttccatt
    31441 gatttttctt tgttcttaga gttgtttgag atgacaaagt tagcaacttg tgttgtttgg
    31501 tttttcttga tgaagttagt aattggtttg atttggatat ctttgttggc aacacgtgaa
    31561 agcaagctgt taccgtagtc agctggtcct actgtttctt cacgaacgaa ccaagtatct
    31621 tgttgaaggt caaaggaagt atcgcttgtt gcgacatctt ttggaatgta gccagcttca
    31681 tagaatttgt gaagagtctt caagtgttct ttgaaacgag gcacttcgta acggtttaca
    31741 actttagtag tatcgccttc aaggtcaata acgaatggaa gcccgtttgc tactgggtag
    31801 tcaaaattat cagatgggat gaaaacttta ccaatagcaa atggtactac gtctggagct
    31861 ttttctttga tttgtttcaa gactggctca agagtttcgt aagaagtaac acctgaaata
    31921 tcgataccat atttggcaag gagagttccg ttgaaggcaa agttttgaga tgatgctacg
    31981 ttggctgcaa ctggtacagc ataaatctta ccatttacag tattaccctt gatataagct
    32041 ggatcaagtg ccttgtaaag gtctttacct tcttttttgt acaattctgt caagtcagca
    32101 taagcacctt tttgagcatt tacaatatag ttatctgcaa aggcgatatc atagttttca
    32161 ccagatgatg tgataactga cattttctta ccatagtcac cccagccaag gtattggata
    32221 tccaatttgg caccaacttt ttcttcgatg attttgttgg catttgctaa caattcatcc
    32281 aagttatctg gtttgtcacc gatttggtac attttgataa caggtttgtc acctgaatca
    32341 gcagcttttt tgctgttacc tgtcaaattt ccacaagcag caagacctgc agccaaagcg
    32401 actacactag cagatgcaaa agcatatttt ttccagtttt tcatgataaa aactcctttt
    32461 tttattttta aacttataaa caatgtaatg atcttatact caatgaaaat caaagagcaa
    32521 actagaaaac tagccgcagg ctgctcaaag cactgctttg aggttgtaga taagactgac
    32581 gaagtcagtt acatatatct acggcaaggc gacgttgacg cggtttgaat ttgattttcg
    32641 aagagtatta acttcacaca agggaagttg ggaactgaga aatgttattt ctcaataagc
    32701 actattcttt cacaccaccg atagtcaaac cttttacaaa gtagcgttgg aaaaatggat
    32761 acaaaatcgc gattggaagg gttgcaacca caaccatggc catacgacct gtttctttcg
    32821 gtagagcaac tcccagttga ccagtcaagc cgaccgcttt ggcaatgtag tccatatttt
    32881 gttggatttg catgagcaaa tattgcaatg gatacaagtt gtcactcttg atgtaaagaa
    32941 gggcgttgaa ccagtcattc cagaaaccaa gagctgttaa gagcgtgatg gttgcgatac
    33001 ctggtagtga caatggcaaa cagatttgga agaaaatccg ggcttcactg gcaccatcga
    33061 tacgagccga ttctagaatg gcttctggaa tggtcttctt gaagaaggaa cgcatcaaga
    33121 tgatgttaaa tggtgagaga agcattggaa caatcaaggc ccaaacagta tccccaagct
    33181 gaagcacacg agtcaccatg atataacctg gtaccaaacc agcgttgaac aacatactga
    33241 gcagaacgaa gatggtaaag aatctgcgat acttaaaggt tgtccgtgaa atagcgtagg
    33301 cataggttgt tgtgataaag acatttgtca atgtcccaac tacggttaca aagacagaga
    33361 tgaagagggc ttgtaggatt ttatccttaa actgtgccaa aaactcaaaa ccgtctaagc
    33421 caaattggga tgggaagaag ctatagccgt attggaggag gcttttctcg tctgtcactg
    33481 aaataatgat aacgaataca aaaggtagga tacaagagag ggcaatcaaa cccgaaatga
    33541 tactgaagaa gatatctgct ttcttactga aggagtgaat gccgacatta tcaatttttt
    33601 cttttttaat tttctttttt gccatattct cctcctttct agaacaaagc tgagtttgga
    33661 tcgactcgtc ttgcaagcaa gtttgatagg ataaccagaa tcaaaccaac aacggattgg
    33721 taaagaccgg ctgctgcagc cataccgata tctgctgtct gagtcaaacc attaaagaca
    33781 tatacgtcca aaacgttggt tacattgtaa agctgaccag cattgtgtgg gatttgatag
    33841 aagagaccaa agtctgcgcg gaagatattt ccgactgcaa ggatggtcaa tacagttaca
    33901 agcggagtca actgaggaat ggttacgttg cgaatacgtt gccacttgct agctccgtcc
    33961 actgtcgctg cttcgtagta ggttggatca attcccatga tcgtcgcata gtacatgaca
    34021 ctgctatatc caaagccttt ccaaatacct aggaaaagta ggagataggg ccagatgccc
    34081 aggtcagcgt aaaaattgac ttctttgaga ccaagacttt ccaatagatg attgaacacc
    34141 cctttatcaa tatttaggaa ggcatctgta aagaaactga tgataaccca agacaagaag
    34201 taagggaaca acatagaagt ttggaaaatc ttcaccattc tcttagaacg gagctcgctg
    34261 aggataatgg caatccctac agatacaact aaacctagaa agataaagcc aagattgtag
    34321 aggacagtat ttcgtgtgat aataaaggcg tctcttgaac taaataagaa tctgaaatta
    34381 tcgagtccga cccatttact atttatgata ctatctatga aaccattact ggtcatgtgg
    34441 tagtctttga aggcaaccac gttcccaaat actggaatgt aaaagaatag aatcaaccag
    34501 agtgtccctg gcaaaaccat caagagaaag atccagttgt ctctcaatgt ttttgaaaac
    34561 tttttcataa tttcctccct ttttattttg atatccatct aaaaattctt ttttagactt
    34621 ttgataacga ttacattatt agtatactcc tatttgcagg ttaggttaaa ctcctaatta
    34681 tagaaaaaac tccacaaatt atgtagcaga tttaaaactt tatcaccact atcaaacaaa
    34741 tgtcctaaat caattgttta ttttatctct attagcccag tgatggcgtc actctgttat
    34801 aagcatccaa caacggggta tactgaaaaa tctccagact agggaactca gagatagttc
    34861 ctaatctgga gatttttaat atgttattag gcgtttgctt tcaacttagc aataacctct
    34921 ttaagattat caatcacctc tgcttcagta tgctcagagc tttttcatct gccaagaaca
    34981 aaactgcttt ttgaagttct ttttgagagt tttcaaggac atccttatct actgtttcaa
    35041 ggtttgagtc tttaagaagt ttacttaatt ccttggctaa tttcttgagt ttgatttgca
    35101 gactcatctt ctcctgctgt ttctttgccc gctgtttgtc ctccatcctt agttgctgac
    35161 tggctttcct taatggactc tagggaagca atggcatctt tgactgtttg caaaatatca
    35221 cgtaaacctt gctctgtcaa actatcatct gcaaaagctt tcttagcctc tgccaaaacc
    35281 agacgtgctg aatctgtggt aggattcgat acacctgtca atgatctcaa aagattttct
    35341 aaggtttgag tctgcttact aatactagac taaaatcaaa aagcattata taacagtgat
    35401 atgaaatcaa ctaaagaaga aatccaaact atcaaaacac ttttaaaaga ctctcgtaca
    35461 gctaaatatc ataaacgcct tcaaatcgtt ctatagtaaa atgaaacaag aacaggacaa
    35521 atcgatcagg acagtcaaat cgatttctaa caatgtttta gaagtagatg tgtactattc
    35581 tagtttcaat ccattatatt tcgtctgatg ggcaaatctt aataaagaga ttatagagct
    35641 tttatagtga tttgaaataa gatgtgaaca actctatcag gaaagtcaaa ttaatttata
    35701 gaaatatttt agcagccaag gtgtactgtt ataaattcaa tacactatac ttggtggttt
    35761 agctctcctg ttttctcttt tagctttaac cagccataaa tggtattacg tgagatttgg
    35821 aaaacgtgtg atgcttctgt tatactacct gttcgctcac aataagagag aactttttta
    35881 cgaaaatcta ttgaatatgc cataaaagat tataccacat tgtgtactat ttttggttca
    35941 ttttactata tcactttgga ctatatcggt ctagttatgt ctcaacgatt atagctggat
    36001 actaagagaa atcaaaaaag cactaaggga gaacgtccca aagtgcttaa tatcaaggcc
    36061 tcaaagcctg tcttattcaa taaaatatta caacatcttg ttgtagaatt caacgacaag
    36121 tgcttcgttg atttctgggt tgatttcgtc gcgttctggc aagcgagtca atgaaccttc
    36181 caacttttca gcgtcgaatg atacgaatgc tggacgtcca agagtagctt ctactgcttc
    36241 aaggattgct ggaactttca atgatttttc acgaactgag atcacttgac ctggagttac
    36301 gcggtatgat gggatatcaa cgcgtttccc gtcaacaagg atgtgaccgt ggtttacgaa
    36361 ttgacgagct tgacgacgag tagtcgcgag accaagacgg taaacaacat tatccaaacg
    36421 acgttccaaa agaagcataa agttgaaacc taggattcca cctttgattt ttgtagcttg
    36481 tacgaacaag ttacggaatt gtttttcacc tacaccgtaa gtgaaacgaa gtttttgttt
    36541 ttcagccaat tgcaaaccat attctgacaa tttagaacgg ttgtttggtc cgtgttgtcc
    36601 tggtacgtag ttacgacgtg ccaattcttt acctgtacct gtaagtgaaa ggccaaggcg
    36661 acgagcttgt ttccaagatg gtcctgtata acgtgacata tgaatgtcct cctgatataa
    36721 ataatatttc ggtggaaata gtcacttaga aagccctgat tcgtgcagat gcccttcgcc
    36781 taaacagcca aggttacttg tcataagaca cctgttgacg agcttcatgc tttcctgctg
    36841 atatttcaca caaaatccat tttaccatga atagctggat ttgtaaaggg gttttaggct
    36901 ttattttcac taccagagag gttgagaacg agggtaaagg tacttcctag accgtactgg
    36961 ctgctgactg tgatttcccc acccaattga tgggccaatt cacgcgcaat cgcaagtcct
    37021 aatccatgac cacctgtctt catgttacgc gaagtttcga cacgataaag gcgtttgaaa
    37081 atattttcca aatcctctgg ggcaatgccc tgcccttcat cggtcacact gattgaaagc
    37141 tggtccttct ccagcttagc caccacttcc agcttggttc ctggagcaga atatttaaaa
    37201 gcgttatcga ccagattcac caagatacga gaaagcttag cataatctcc ctcaatccgg
    37261 gcagactctg ggattacctg caagtggaca tctcttctct cctgctcaat caaaaactga
    37321 aattcactca tgcactcaat taagagcttg tccagaaaaa tactgtcttt actggtagtt
    37381 tccacctgat ttctagctgt gtttagggtc aaaaaattca actcctcaac cagtttattg
    37441 agcctctccg tctggcgtcc aatggttgct agataatgag cttgctccga ctccttgata
    37501 atcccatcca aaatcccttc taccgtcgct tggatcgaag tgataggggt cttaatatca
    37561 tgcgacaact gggcaatcat caagcccttt tctcgttcgc tttcttccaa ggaatcaaag
    37621 cttacctgca aatcatggga catctcatta aaagtttgcc ctaattgctg aaattctaca
    37681 ggaccttgaa cctccaaatt tgaaggaaaa tccttggccg ctacccgctt ggcatgctcc
    37741 ttgagtttgc ccaacgacgt aaagactggc aataggagaa agagactaat cccagcaccg
    37801 acaaggctgg caacgatggt catcccaagc aaaaagtaaa tctcgccttt cgcaatcagc
    37861 attttttgaa cagcccaaaa aacaaccaaa atggttaaga gggttgaaat aatatatcca
    37921 accaaaatat aactttttag tttcatattt gtcctctcgg tttctctatc ttatatccca
    37981 acccccaaac tgtcttaata gtgggagttt ggtcactact atattttgcc agctcctgtc
    38041 gaagagcatg gatatgcaca ttcaaggtat tggtgtcatc cacgtagtct tctttccaga
    38101 tcttttcata gaggtctgtc ttggagaaaa ctcgctctgg attgctagct aaaatccaca
    38161 gcaattcaaa tgatttaaca gttaaatcca gcatttcttc tcctatttga acttcatgac
    38221 tactatgatt catttttaga ttgccaaggg aaatcagctc tgtttcgccc ccacgatgaa
    38281 ggcggcgcaa aatattgtgg acacgcaaaa ccagctcacg tgggctaaaa ggcttagcaa
    38341 taaaatcatc tgctcccaag ctcaggccgt aaatcttgtc ctgttcactg gtcttagcag
    38401 taataaatag gaaaggctgc tctggtgata agtattgaac ctcactgatt aaatcataac
    38461 catccatccg aggcatcatg acatctgtga taatcaaatc aatcggtttt ttcttgaaca
    38521 gctctagcgc ttccagtcca tcatgggcta ccaagacctg ataacctgcc tgaattaagt
    38581 atctctgatg aatatctgtg atttctacct cgtcgtcaac gagtaaaatt gtctttccca
    38641 tctgtctctc ctttgataaa aacagtgcta tactgcttta agtataacac tatttttatt
    38701 gttttacgat gattttaatc tgctattgaa agaaagtcct gattgctgat tgagttgtta
    38761 aactaatctt agtcagccca ctatccatca tttatttcct tagctgatta gttttcttct
    38821 tttttgtttt tcaaacctag accaccgagt aaacctgcaa gcgcaagccc aaggaaacca
    38881 atacttgcca ttgatgtttg agtctcacca gtatttggta gcatagcttt atcctctgac
    38941 atcatcgtat cagacatctt gttagcagaa gcagccatgt tttcacctgc catcgtgttg
    39001 gtagaacttg tcatggtgtc agcaggcatg ctatctgtag tacctgtagc atgattgtga
    39061 ttcatcggag tcacgccaga accagagtta gaaggtgata atgaaccatt tgctgtgtct
    39121 gatgtttctt taacatttat
//
LOCUS       DAVDWT010000022        37911 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVDWT010000022 DAVDWT010000000
VERSION     DAVDWT010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37911
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.022"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            5..1597
                     /locus_tag="VUW50_001275"
     CDS             5..1597
                     /locus_tag="VUW50_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7983167.1"
                     /translation="MKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMNLERFLKLS
                     IYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHSKSSGTYLS
                     WLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLMIMLLVPKI
                     FASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAGILLKMVIQ
                     RKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTGVIFTALGE
                     LGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNICYKYGDKEI
                     LKNLNFCFQRNEKYLILGESGSGKSTLLKLLSGFLRDYSGELRFCGDDIKKTSYLNMV
                     SNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNILDYYVGDD
                     GRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDREDLTVIIVT
                     HAPHPELKQYFTKIYQFPKDFI"
     gene            2061..2286
                     /locus_tag="VUW50_001276"
                     /pseudo
     CDS             2061..2286
                     /locus_tag="VUW50_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2298..3047
                     /locus_tag="VUW50_001277"
     CDS             2298..3047
                     /locus_tag="VUW50_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET7983168.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPCVSDSSMVSYMGKTKELKMYQEELDEKYYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            3060..4232
                     /locus_tag="VUW50_001278"
     CDS             3060..4232
                     /locus_tag="VUW50_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET7983169.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGILP
                     SKNEELMKFFCENNLNITFFDTFSEIKNNIDYYIIAIPTDYDEKIGSFNTYEIEQTVS
                     KILRVKPNGKIILKSTVPIGFSNKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVV
                     GDETVEGRKIAELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELD
                     FFAERRSLNAEVVIKGVCLDPRIGNFYNNPSFGFGGYCLPKDTKQLKKEFIEINAPVI
                     EAIDISNTNRKQFIVKQILERKPKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIK
                     ILVYEPNLNVSIDNVIFEKNFELFTKQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
     gene            4628..5491
                     /locus_tag="VUW50_001279"
     CDS             4628..5491
                     /locus_tag="VUW50_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET7983170.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACDGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TLPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFLYIPIFE
                     AELESMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFTQSLQVLENPQLTSLFE
                     LRLQQYKKLID"
     gene            5777..5974
                     /locus_tag="VUW50_001280"
     CDS             5777..5974
                     /locus_tag="VUW50_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HET7983171.1"
                     /translation="MLNLQFAETMELTEAELETVYGGEFGNNAVIPAGAWGGLGTSWS
                     ITNFWKKYFNHDSSTVNRRHY"
     gene            6042..6707
                     /locus_tag="VUW50_001281"
     CDS             6042..6707
                     /locus_tag="VUW50_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7983172.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            6779..8005
                     /locus_tag="VUW50_001282"
     CDS             6779..8005
                     /locus_tag="VUW50_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7983173.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLT
                     ILLITISETLPQILQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(8359..8433)
                     /locus_tag="VUW50_001283"
                     /pseudo
     CDS             complement(8359..8433)
                     /locus_tag="VUW50_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            8504..9160
                     /locus_tag="VUW50_001284"
     CDS             8504..9160
                     /locus_tag="VUW50_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET7983174.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            9150..9473
                     /locus_tag="VUW50_001285"
     CDS             9150..9473
                     /locus_tag="VUW50_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET7983175.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            9577..10407
                     /locus_tag="VUW50_001286"
     CDS             9577..10407
                     /locus_tag="VUW50_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7983176.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            10561..11415
                     /locus_tag="VUW50_001287"
     CDS             10561..11415
                     /locus_tag="VUW50_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7983177.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            11515..12888
                     /locus_tag="VUW50_001288"
     CDS             11515..12888
                     /locus_tag="VUW50_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7983178.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            12881..13942
                     /locus_tag="VUW50_001289"
     CDS             12881..13942
                     /locus_tag="VUW50_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HET7983179.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            13944..14636
                     /locus_tag="VUW50_001290"
     CDS             13944..14636
                     /locus_tag="VUW50_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7983180.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(14666..15235)
                     /locus_tag="VUW50_001291"
     CDS             complement(14666..15235)
                     /locus_tag="VUW50_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET7983181.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIAFFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            15370..15984
                     /locus_tag="VUW50_001292"
     CDS             15370..15984
                     /locus_tag="VUW50_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET7983182.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            15986..17632
                     /locus_tag="VUW50_001293"
     CDS             15986..17632
                     /locus_tag="VUW50_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7983183.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLAPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRNFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            17644..18930
                     /locus_tag="VUW50_001294"
     CDS             17644..18930
                     /locus_tag="VUW50_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7983184.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLTSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            18982..19515
                     /locus_tag="VUW50_001295"
     CDS             18982..19515
                     /locus_tag="VUW50_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7983185.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDETMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            19593..20063
                     /gene="nrdI"
                     /locus_tag="VUW50_001296"
     CDS             19593..20063
                     /gene="nrdI"
                     /locus_tag="VUW50_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HET7983186.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(20356..21618)
                     /locus_tag="VUW50_001297"
     CDS             complement(20356..21618)
                     /locus_tag="VUW50_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET7983187.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(21928..22386)
                     /locus_tag="VUW50_001298"
     CDS             complement(21928..22386)
                     /locus_tag="VUW50_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HET7983188.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(22383..22823)
                     /locus_tag="VUW50_001299"
     CDS             complement(22383..22823)
                     /locus_tag="VUW50_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7983189.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLIEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            23407..24273
                     /locus_tag="VUW50_001300"
     CDS             23407..24273
                     /locus_tag="VUW50_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7983190.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYQVNHLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAYFLYKLEENMSMT
                     LKVEHYIADITE"
     gene            24610..25167
                     /locus_tag="VUW50_001301"
     CDS             24610..25167
                     /locus_tag="VUW50_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase family
                     flavoprotein"
                     /protein_id="HET7983191.1"
                     /translation="MDKKNLLVLVTGSVGASNVDTYLYYIKKFYNVKVILSENSKKFI
                     SKELISYFCDKVYDEIFVEEVVPHVFLPYESDLLLILPATANVIGKIANGIADDLVTA
                     TVLNFNKKIIFCPNMNSTMWDNHIVQRNVSILKELGHIFLFESKKTYEVGLRKAIDST
                     CSMLQPQSLVKELIKLENIVLEEGH"
     gene            25388..26494
                     /locus_tag="VUW50_001302"
     CDS             25388..26494
                     /locus_tag="VUW50_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET7983192.1"
                     /translation="MINKKIQQVVLESLQNFLNGNFISPCVVYDFGLLETVLDEFKNQ
                     IPVTFNYQLFYAVKANSNEKILEFLVDKIDGVDVASLSELDVAKKFFPPTQISVNGPA
                     FSYETLYNLIKKQYKVDINFLEHLQQFSPKESVGIRVTEPDELNNRMSRFGINICSDN
                     WTSNLQNPLITRLHFHFGEKDDKFIVKLDKILFKLQEINKLREVREINLGGGFMKLFM
                     ENRLKEFFLSLMEIYKKYDIDSTVTTIIEPGSAITSFSAYMITSPVNVSEVNEQQVIT
                     LDTSIYTNTLWFVPHIITTLNSSSKERYSTILYGNTCYEHDKYKMKVSLPRLTQNSSI
                     VFFPVGAYIKSNHSNLHRNDFMREVYLWTKNLTY"
     gene            26470..27861
                     /locus_tag="VUW50_001303"
     CDS             26470..27861
                     /locus_tag="VUW50_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983193.1"
                     /translation="MDKKLDILDKVKEYLGNKTTQILDNQYKEFLKLNDIRRAFGISE
                     KVLNNSFNFTSKEFNDLINNENYLFEYACRIREEWRKKCFNHSYRFLCSPIITDDFLN
                     TKTLRSSQIEYKYERYLSKSSIGDRAVDGFVSFNTLTANGMSAIKLCLEILNSIFFKK
                     KIDLLYSTGYYETRFLLNNLAKSGISCYEVSNCELDKDKFYNVFMMEPNRADLTLQKT
                     DFKIVEYFVKYKNNSIKVVILDISYQGSNFKLVEFLEKFKFANVIIFVVRSLIKLDQM
                     GLELTNGGIIEVFIPNHLRKLKNFIEEEFNKFRNSHGANLSLYEYCLLDNSLTLKNDW
                     NYSDLVMKFTSNFYADIKDLFMENSDIEIIHEEGVPFVFLDLIGEGKKEYEMFFQWLN
                     FFYKQLGITLYARNSFGFRNLTVEYFGIIGTERYIFKICPGVYKGLSYYLMKFLLKSF
                     SNEYLKTTDEVNR"
     gene            27858..29036
                     /locus_tag="VUW50_001304"
     CDS             27858..29036
                     /locus_tag="VUW50_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7983194.1"
                     /translation="MKNLIKLLIIRLIVNLADSVFYIVALWHVSNNYSSSMFLGIFIA
                     VNYLPDLLLIFFGPVIDRVNPQKILIISILVQLAVAVIFLLLLNQISFWVIMSLVFIS
                     VMASSISYVIEDVLIPQVVEYDKIVFANSLFSISYKVLDSIFNSFASFLQVAVGFILL
                     VKIDIGIFLLALFILLLLKFRTSNANIENFSFKYYKREVLQGTKFILNNKLLFKTSIS
                     LTLINFFYSFQTVVVPIFSIRYFDGPIFYGIFLTIAGLGGILGNMLAPIVIKYLKSNQ
                     IVGVFLFLNGSSWLVAIVIKDYTLSLILFFVCFMSKGVFNIIFNSLYQQIPPHQLLGR
                     VNTTIDSIISFGMPIGSLVAGTLIDLNIELVLIAISIPYFLFSYIFYTDNGLKEFSIY
                     "
     gene            29461..29694
                     /locus_tag="VUW50_001305"
     CDS             29461..29694
                     /locus_tag="VUW50_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET7983195.1"
                     /translation="MISIDLGGTNIKITVLSNDGEIETLWSITTDTSEKGSQIISDII
                     SSIKNKLTERNIPDSDLLGIGMGSCSSYFPCKS"
     gene            29977..31926
                     /gene="mutL"
                     /locus_tag="VUW50_001306"
     CDS             29977..31926
                     /gene="mutL"
                     /locus_tag="VUW50_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET7983196.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(32251..32718)
                     /locus_tag="VUW50_001307"
     CDS             complement(32251..32718)
                     /locus_tag="VUW50_001307"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET7983197.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(32719..33924)
                     /locus_tag="VUW50_001308"
     CDS             complement(32719..33924)
                     /locus_tag="VUW50_001308"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET7983198.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERSGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(33944..34579)
                     /gene="ribE"
                     /locus_tag="VUW50_001309"
     CDS             complement(33944..34579)
                     /gene="ribE"
                     /locus_tag="VUW50_001309"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET7983199.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(34564..35664)
                     /gene="ribD"
                     /locus_tag="VUW50_001310"
     CDS             complement(34564..35664)
                     /gene="ribD"
                     /locus_tag="VUW50_001310"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET7983200.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(35798..35912)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            36069..36662
                     /gene="ruvA"
                     /locus_tag="VUW50_001311"
     CDS             36069..36662
                     /gene="ruvA"
                     /locus_tag="VUW50_001311"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET7983201.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            36672..37235
                     /locus_tag="VUW50_001312"
     CDS             36672..37235
                     /locus_tag="VUW50_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET7983202.1"
                     /translation="MTKRCSWVKMTNPLYITYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            37232..37909
                     /locus_tag="VUW50_001313"
                     /pseudo
     CDS             37232..37909
                     /locus_tag="VUW50_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
BASE COUNT        11629 a         6377 c         7695 g        12210 t
ORIGIN      
        1 aaatataaaa aaatttagtt tattaacgat atttattgtt gctggtcaat tattgctaat
       61 ttatgcagca actattaatg ctctggtgtt gaatgaatta attgcgatga atttagagcg
      121 gtttttgaaa ttgtcaatct accaaatgat tgtctggtgt gggataatat tccttgactg
      181 ggtagtgaaa aattatcagg ttgaagtgat ccaagagttt aatctagaga ttagaaatag
      241 agttgccaca gacatctcta actctaccta tcaagaattt catagtaaat catcaggaac
      301 atatctttcg tggctaaata atgatgttca gactttaaat gatcaggctt ttaaacaact
      361 ttttttagta ataaaaggaa tttctggtac tatatttgca gttgtgactc ttaatcacta
      421 tcattggtca ttgactgtag ccaccttgtt ttcattaatg attatgctac ttgtaccaaa
      481 aatctttgca tcgaaaatgc gagaagttag tctaaattta actaaccaaa atgaagcttt
      541 tttaaaatct agtgagacta tattgaatgg atttgatgtg ttagcgtcct tgaatctttt
      601 atatgtattg cctaagaaaa ttaaagaagc aggaatttta ttaaagatgg ttatacaaag
      661 aaagacaact gtagaaacgt tagcaggcgc tattagcttc tttctcaata ttttttttca
      721 gatatctctc gtttttttaa caggctatct tgcaataaaa ggaatagtga aaattggtac
      781 tattgaagca ataggagcac taacaggtgt tatttttaca gcgctaggtg aattaggagg
      841 tcaattatcc tctattattg gtacgaagcc tattttttta aaattgtatt caattaatcc
      901 aattgagtca aataaaatga atgatatcga accaaatgag gtgaatagag attttccgtt
      961 atatgaagca aaaaatattt gctataagta tggagataaa gaaatattaa aaaacttaaa
     1021 tttttgtttt caacgtaatg aaaagtattt aattttaggt gaaagtggaa gcgggaaatc
     1081 tacattatta aaattattga gtggcttttt aagagattat agtggagaat tgcgattctg
     1141 cggggatgat ataaaaaaaa cctcctattt aaatatggtt tcgaatgttc tatatgtaga
     1201 tcaaaaagct tatttgtttg aaggtacgat tagagataat attttattgg aagaaaatta
     1261 tactgatgaa gaaatactac agtctttaga gcaagttggt ttgagtgtaa aagattttcc
     1321 taataacatt ttagattatt atgttggtga tgatgggaga ttactgtcag gagggcagaa
     1381 acaaaaaatt actttagcta gagggctaat tagaaataag aaaatagtat taattgacga
     1441 gggaacttct gctatcgata ggagaacttc gttagcgatt gaacgtaaga tattagatag
     1501 agaggatttg actgtcatta ttgttaccca tgctccgcat ccggaactta aacaatattt
     1561 tactaagata tatcaatttc caaaggattt tatttaagaa tataggggag ggaaacatga
     1621 gattctattg tgaagcttat gaggtaatta ctgaagagaa aaaagtatca tttttttatg
     1681 tagatgaaaa acccttaagg tatctgttat ttgactatat atttctacta gttctagcta
     1741 aaaaaaatat cccaatctta tgaactgcac cccaaaagtt agatttattc tgtctaactt
     1801 ttggggtgca gttcattttg catagttttg gcattcatcc tagatatcca tataaaatgt
     1861 ctgagattgt tgaaatgtct aaaaaaatat cagatctaaa cgataaaaaa gaatttcaca
     1921 aaaagaactg tgagttgagg tttcaagata gtgttcttga taacataaat agtgtcccgg
     1981 gtacaactca tttatttagt tgtttaaata ttcaaacatt agtaaaaatt attttatata
     2041 tttttcgata caacagttgt atgaaaatat ttaattttag agataaagag cttatctttt
     2101 ttaaataaga ttgtaaatgg gctgatacaa aatattgagg agaatttaga ggatgatatt
     2161 gaaaggatat tgaaatattt atatatatgt ttatttaacg aaatattcat tattaagaat
     2221 aaggtgaatt tttttgatga tgtggaattt aatcagacac ttagtgagtt tttagataaa
     2281 ttgtagaggg aatggttatg agtaacaata ctaagtatat ctttttatct ccacatttag
     2341 atgacgcaat tttttcctgt ggtgattata tttcaaaatt aacttcagaa ggagaaattg
     2401 tacttgttat tactatattt tctggttacc ctttgagcca acagctacag ccctctgcta
     2461 agcaatttca taagttgtgt aaccttggga aatatccgat tgaagaaagg aagaaagaag
     2521 acaggttagc ttgtgaacgt ctacaatgtg attttagaca cttatcgtac tatgaatgtc
     2581 tctataggaa agatagaaat ggcaattttc tataccgtca tatctatagt gaattaaaaa
     2641 atgaggacac attaaagaat gatatcataa aagagctgtt aatgcattta gacgacaaat
     2701 gtgtcgtata ttgtccgctt tcacttggtg accatgttga tcatgttttt gtgaatagta
     2761 ttggaagggc gcttgaattt atgcgttata aagtaatata ttatgaggac ttcccatgtg
     2821 taagtgatag tagtatggtt agttatatgg gaaaaactaa agaattaaaa atgtatcaag
     2881 aggaacttga tgaaaaatat tatattgata gaatatcatc aatattgtgc tataaatctc
     2941 agattttgat aatctggaaa tcagttgaaa aacttttaaa caatattaaa gagttgtatt
     3001 taaggaatgg ggcagcttat tctattagat tttggattaa gaaataagga agtagtttta
     3061 tgaatatagc agtaattggt ttggggcatg ttgggctggc ctacgcgtta ctatttgcat
     3121 ctaaatataa agttgttgca tatgatatag attctgtaaa aataaataat ttaaaaaagg
     3181 gcattcttcc atctaaaaat gaagagctta tgaagttttt ttgcgagaat aacttaaata
     3241 ttactttttt tgatacattt tctgaaatta aaaataatat tgattattat attattgcga
     3301 ttccgacaga ttatgatgag aaaattggta gttttaatac atatgaaatc gaacaaacgg
     3361 tatcgaagat tctgagggta aaacctaatg gaaagattat tttaaagtca acagttccga
     3421 tcggcttttc aaacaaatta aaaaggctgt ttgatacaaa aaatatcatt tttgtccctg
     3481 aatttttgag agaaggttgt tctatatatg ataatttata tccaagtcgc atagttgttg
     3541 gagatgagac agttgaagga agaaaaattg cagagttgtt cctttcgatt agtactcata
     3601 gtactgccaa tattaaaaat gttatgttag tttctcctac tgaagcagaa gcaattaagc
     3661 ttttttctaa cacattctta gctctccgtg ttgctttttt taatgaacta gatttttttg
     3721 ctgagaggag aagtttaaat gctgaagttg taataaaagg tgtttgttta gatccaagaa
     3781 ttggaaattt ttataataat ccttcttttg gatttggagg atattgtctt cccaaagata
     3841 ctaaacaatt aaaaaaagaa tttatagaaa taaatgcccc agtgatagaa gcgattgata
     3901 tttcaaacac aaatagaaaa cagttcatag ttaaacaaat attggaacga aagccaaaga
     3961 tagtaggaat atataaattg gggatgaaat ataattcaga taattacaaa gagtcagcta
     4021 ttttaagtat aattaatgaa ctattgattg tcggtataaa aattttagta tatgaaccaa
     4081 atttaaacgt tagtatagat aacgtaattt ttgaaaagaa ttttgaacta tttacaaaac
     4141 aaagtgatct aattgttgca aatagatggg ataggggcct tgaagcttat aaagataaag
     4201 tttacacaag ggggatctgg ataagagatt aactattgat attaaaaagg caataattta
     4261 taaaatttgt acaaaaaaat gttaaatttt cacattttca tagtatactg tttaatagaa
     4321 agtaggttct ttgtaaccgc tccattataa aatcctttca ctcttccggt atactattag
     4381 gaaaaacata actggaggat attttgagct cacacctcat cgttccagtg gctgttcccc
     4441 aagctcgtgt actgcccaaa aaagttagac attctattta gaacgaggat tgagttctgt
     4501 attgtacaag gctcggtcct tttagagtca gcttaaggct ggctttttta ttttttctaa
     4561 ctatcagaat attttgacaa atgaacacaa ataatgatat aatatgcaaa agctaggagg
     4621 tggtaggatg attgaaaaaa tggaattggg ggaattttac aaggaattac gtttggcgag
     4681 aaagctcaag cagtcagatg tggcttgtga cgggctaaca gcttctcagt tgtccaagtt
     4741 tgagctaggg cagtccatgt tgtctgctga taagctcata ttagccatcc aagggatcaa
     4801 tgtgaccttt gatgagtttg ggcacaagct caacaactat caagaatctc cacatatgca
     4861 gattggtaga aaggttgtgg atcgctttgc tcatcaagat atagctggct tagagcaact
     4921 gttggaggaa gtcgagcaag aacagatggc agagacttat cgtcgtttga atgctattgt
     4981 aatcaaagat gccatccact cactagataa aagctaccca ctagcagagg aggatagcga
     5041 gtttttgacc acctatctct atgctatcga gtcttggacc tggtttgaac tctatctctt
     5101 ttgcaatact ctgcccttct tgagcaatca agatctgatc tttttatcaa cctccttact
     5161 cgaaaaatcc aaagaattta aagagttagt acacaatcga ttgtatatga agcaaggact
     5221 cttaaatatc ttatcagaac tcatggagcg aaaacttttc ttatacatcc caatcttcga
     5281 agccgagttg gagagcatgt tgcgtccgta cgatgttttt gaaaaagtat cgtggcaatt
     5341 tttaaagaag atgagtgtct ttcttcaaac aaagggaagc aatcaaaaag agattgaacg
     5401 ctttacccaa tctctgcagg tattagaaaa tccacaatta acatcccttt ttgaattgcg
     5461 tttgcagcaa tacaaaaagc ttatcgatta gttagaaatg gcaaaataat aataatctca
     5521 gcaacgatcg tcaataatgt taaaacttgt ttcttcatat tacttccctc ctcattagta
     5581 ataactttat tataccaggg aaataatcaa atctaccaaa atcgcaaatc tgaaatttct
     5641 ataagaaaaa atatcaaata tgcgattttc taaaataagc caatttccgt gttatactgt
     5701 ccttgtaaaa catttgaagc aaatcctagg tcgcagaagt ggtttacaaa tgaagataat
     5761 tgaaggagtg taaaagatgt taaatttaca atttgcagaa acaatggaat tgacagaagc
     5821 tgagttggag acagtttatg gaggggaatt tgggaataat gctgttatcc cagccggtgc
     5881 ttggggaggt ttaggaacgt cttggtcaat cactaatttc tggaagaaat attttaacca
     5941 tgattcttcc actgttaatc gtcgccatta ttgatattca gagtccttgc ctttttcttg
     6001 ggtagggact gtctttattt catagtatag gagaaaatag aatgaaaaag ataatctcac
     6061 accgctactt tattatagtt tttctactag ttattgctga ccagaagttc agtgttttag
     6121 ttttacgcag cgaccttgtt gctgggctat ctgactttgc ctattatctg tcggatatga
     6181 tgttgaattt tcttgtggtt ttatttgctc ttattgctat gatttggtcg gggaaatggc
     6241 agaaaatcaa tagtagaaag tttaaaggat cctatctttt ctattccttc ctagctctgc
     6301 ttgcttttgt tgcttggaat tttgtcacct tttttctttt cccacctact cgaaatgaaa
     6361 tttcttatca acatgctgct cctactttta caggagctac ggcatttttg atgtattttt
     6421 tttatcctgt gattgcaggt cccatttttg aagacatgat ttatcgtgga ttagtgatga
     6481 ctgctctgga aaaaggaaag aaatgggggc tagatgtact tggttccgct gttttatttg
     6541 gggtctcgca cattagtaat cacggttggg tcttgacaga ctttgtcttc tatatgggtg
     6601 gaggtcttat atttgcagtc ttatttagaa tgacaaagtc aatttattgg cctattggac
     6661 tgcatatagt ctacaatggc attggtcagc ttttgatgtt actgtaattt tggttgttag
     6721 tagtatcgcg gtctatcctt ggggaaagat tcccatttga acttaagaag gagttgtgat
     6781 gaaagtattt cttcaaaata gagattttag gcaattaact atcaaccagt ggatttcaac
     6841 gcttggggat acgatttttt atctggcctt tctgaattat gtggcagatg cgtcttttgc
     6901 acctttgacg attttactca ttacgatttc agaaaccctt ccccaaattt tacaaatctt
     6961 tctgggagtt ttggcagatt ttcaacatca tcgtgtcttg aaatacacag tcattagttt
     7021 tgcaaaattt ttgctttact ctatcgtttc tttatcactt tcagggcagt ctttctcctt
     7081 gttgctagta gcatttattt gtctgcttaa cctcttgtct gacacattga gttacttttc
     7141 aggagccatg ctcactccga ttttcattag aattattggg caagaccatc tggcagaagc
     7201 tattggcttt aaacagtcaa ctgttagttt agtgaaaaca atcagtaata tcctaggagg
     7261 agtcttacta ggcattctat ccatccagtt tatttcctta ctgaacgctc ttaccttttt
     7321 aatcgcattt ttaggtatcc ttttcataaa aactgacctc ttgaaagtag aaaaaacgat
     7381 tagctatcaa gaaggactct ctgtaaaatc cttttgccag catttgctcc aatcatcgaa
     7441 attgatatgg aatatgaata aggtgctctt ggttttgttt attatctcta ctagtcaagc
     7501 agtgataaat gttacagttc ctgtctctac tctatttttg aggaatcaac cctttttgaa
     7561 tttacaaaca ggtcaatctc ttgccttgct atccactttt gaactgtcag ccctgattgt
     7621 cggaagcctt gtaagtggct atctgcaaca taccatttcc ataaaaacag ctctctatgc
     7681 ctcacttgtc atccagttgc ttcttctagt aggatttgcc acagttcgtt ttgactggat
     7741 tctcatcttt agtaccttgg atgccttttt cgcaggtgtt ctctctccta gattgcaaga
     7801 actcgtcttt aaacaaatac ctgaggagtc aatgggagca gttcaatcct ctatcggtgc
     7861 cattacggtt gttttaccta gcttatttac aatagctttg gtaaccattg ctactagctt
     7921 tggaactctg gcagttagct ttgttttatt gctatttctt ctagttgcct ttgttatgct
     7981 tctgaatatt cgtgaaagta tttagtggag aatttctata atatatgttt gagactggat
     8041 tgccagtctt tttatgatgg tcaaaatcaa cagacttttt tctcttttct gaatgtgtta
     8101 taatagactg aatctaaaat agtacgaaac aattgctaaa acatttatag aaattaattt
     8161 tactttccca atcgatttgt tctcatctta tttcaatctg ctatataata gtccatgctg
     8221 tggctggatt cttttcagcc tacttatact cttcgaaact ctcttcaaac cacgtcagct
     8281 tcactttgcc gtatatatgt tactgacttc gtcagttcta tccacaacct caaaacaccg
     8341 ttttgagctg acttcatcag ttctatctgc aacctcaaaa cagtgttttg agcaacctgc
     8401 ggctagtttc ctagtttgct ctttgatttt cattgagtat tattaagaca atatgaggag
     8461 aaagatgaaa gaaaaaggat tttaggaggg ggcgcaggca gccatgccaa cggctcttgg
     8521 ctatgtcagt atcggcctgg cctgtgggac tatcggtgcg ccctatgtga cacctgttga
     8581 gatgggcttg atgagtctct ttgtttatgc tgggagtgcc cagtttgcca tgttggcact
     8641 gattgtggtt caagctcctg tggcagctat tgctatgacg gtttttctaa tcaacttgcg
     8701 tctctttttg ttgagtttac acgcatcgac ttatttccgt cataccagtc tttggtacaa
     8761 tatcggtatg tctagtatct tgacagatga gacctatggc gttttgatgg gtgaattggc
     8821 ccatacagac aaggtaaatc ctatgtggat gcacggaaac aatcttaaca gttatgtggc
     8881 ttggtttgtg gggacagtag tcggaacggc tctgggtggc ctgctaccaa atccagaaat
     8941 ctttggcttg gattttgccc tggttgggat gtttattggt atttttgctt cgcaatttca
     9001 gattatgcaa agacggattc ctgtccgcaa tctgctcatt atcctagcag ttgttgcggt
     9061 gtccttcttt ttgctcttga cagtggtgtc tcagtcacta gctgttctgt ttgcgacgct
     9121 acttggttgt agcatggggg tggttttaga tggtcagtaa gtatctttta ttagcagtta
     9181 ttttctctgg cttggtgact tggattctcc gtatgattcc cttcatctta gtcaagtata
     9241 agggcttgcc tgcaatcgtt gagcgttttt tgaagttctt gcccgtttcc attatctttg
     9301 ccttgattct ttcaagcgta gtgacaggta aggttgggag ccttcctcaa attaaatggc
     9361 tagacttctt agccgtcttt ccaacagctt gggtagcctt tcgctaccgc aatctagtcg
     9421 gaacagttct ctttggagtg gttttgattg ccatcttgcg tttggtcttt taaattaccc
     9481 ataaaaaaaa cttatcacag agatagatat catataatgg cgtaaatgct ctttttctgt
     9541 taagattata aggtattcta ttttggagga aatgacatga aaaaaatcgt taaatactca
     9601 tctcttgctg ccctaggact tgttgctgca ggtgtgcttg cggcttgctc agggggtgct
     9661 aagaaagaag gagaagcagc tagcaagaaa gaaatcatcg ttgcaaccaa tggatcacca
     9721 aggccattta tctatgaaga aaatggcgaa ttgactggtt acgagattga agtcgttcgc
     9781 gctatcttta aagattctga caaatatgat gtcaagtttg aaaagacaga atggtcaggt
     9841 gtctttgctg gtcttgacgc tgaccgttac aatatggctg tcaacaatct tagctacact
     9901 aaagaacgtg cggagaaata cctctatgcc gcaccaattg cccaaaatcc taatgtcctt
     9961 gtcgtgaaga aagatgactc tagtatcaag tctctcgatg atatcggtgg aaaatcgacg
    10021 gaagtcgttc aagccactac atcagctaag cagttagaag catacaacgc tgaacacacg
    10081 gacaacccaa ctatccttaa ctatactaag gcagatttcc aacaaatcat ggtacgtttg
    10141 agcgatggac aatttgacta taagattttt gataaaatcg gtgttgaaac agttatcaag
    10201 aaccaaggtt tggacaactt gaaagttatc gaacttccaa gcgaccaaca accgtacgtt
    10261 tacccacttc ttgctcaagg tcaagatgag ttgaaatcat ttgtagacaa acgcatcaaa
    10321 gaactttata aagatggaac tcttgaaaaa ttgtctaaac aattcttcgg agacacttat
    10381 ctaccggcag aagctgatat taaataattt cttgaaatca tccattctga gtaaggtggg
    10441 tgatttctca gtttttaggg atatagtttt aaactatata tctgattatc taataacaat
    10501 ttgtgaaatc aaacaaattg tgagatacta gtacggtatt atttttaagg agaaagaatc
    10561 atgaaaatca aaaaatggct tggtctagca gcccttgcta cagtcgcagg tttggctctt
    10621 gcagcttgcg gaaactcaga aaagaaagca gacaatgcaa caactatcaa aatcgcaact
    10681 gttaaccgta gcggttctga agaaaaacgt tgggacaaaa tccaagaatt ggttaaaaaa
    10741 gacggaatta ccttggaatt tacagagttc acagactact cacaaccaaa caaagcaact
    10801 gctgatggcg aagtagattt gaacgctttc caacactata acttcttgaa caactggaac
    10861 aaagaaaacg gaaaagacct tgtagcgatt gcagatactt acatctctcc aatccgcctt
    10921 tactcaggtt tgaatggaag tgccaacaag tacactaaag tagaagacat cccagcaaat
    10981 ggagaaatcg ctgtaccgaa tgacgctaca aacgaaagcc gtgcgcttta tttgcttcaa
    11041 tcagctggct tgattaaatt ggatgtttct ggaactgctc ttgcaacagt tgccaacatc
    11101 aaagaaaatc caaagaactt gaaaatcact gaattggacg ctagccaaac agctcgttca
    11161 ttgtcatcag ttgacgctgc cgttgtaaac aataccttcg ttacagaagc aaaattggac
    11221 tacaagaaag cacttttcaa agaacaagct gatgaaaact caaaacaatg gtacaacatc
    11281 attgttgcaa aaaaagattg ggaaacatca cctaaggctg atgctatcaa gaaagtaatc
    11341 gcagcttacc acacagatga cgtgaaaaaa gttatcgaag aatcatcaga tggtttggat
    11401 caaccagttt ggtaataaga aacagggagg tgggagagaa aattccacct cttgcttttg
    11461 tatagagcac ggagtgtaaa gaagactatt cgttcataga aaggtagaga gaatatggtt
    11521 tttcctagcg aacaagaaca gattgaaaaa tttgaaaagg atcatgtagc ccagcattat
    11581 tttgaggttt tgcgtacctt gatttctaag aaatcagtct ttgcccagca ggttggactc
    11641 aaggaagtcg caaattatct gggtgagatt ttcaagcgtg ttggagctga agtggagatt
    11701 gatgagagct atacagctcc ctttgtcatg gcacatttca agagttcgcg tccagatgcc
    11761 aagaccttga ttttctataa ccactatgac actgtgccag cggatgggga tcaggtctgg
    11821 acagaggatc cttttacgct ttcggtccgc aatggcttca tgtatgggcg tggggttgat
    11881 gacgacaagg gtcatatcac agctcgcttg agtgctttga gaaaatatat gcagcaccat
    11941 gatgatttac ctgtcaatat cagctttatc atggagggag cggaggaatc ggcttcaaca
    12001 gacctagata agtatttgga aaagcatgca gacaaactcc gtggggcgga tttgttggtc
    12061 tgggaacaag ggaccaaaaa tgccttggaa cagctggaaa tttctggtgg caataagggg
    12121 attgtgacct ttgatgccaa ggtaaaaagc gctgatgtgg atatccactc gagttatggt
    12181 ggtgttgtgg aatcagctcc ttggtatctc ctccaagcct tacagtctct tcgtgctgcg
    12241 gatggccgta tcttggttga aggcttgtac gaagaagtac aagagcccaa tgaacgagaa
    12301 atggccttgc tagaaactta tggtcaacga aacccagagg aagttagtcg gatttatgga
    12361 ttggagttgc ctctcttaca ggaggagcgg atggcctttc taaaacgttt ctttttcgag
    12421 ccagcgctta atatcgaagg aatccagtct ggttatcaag gtcagggtgt taagactatt
    12481 ttgcctgcag aagccagtgc caagctagag gttcgtctgg ttccgggtct agaaccgcat
    12541 gatgttctgg aaaaaattcg gaaacagcta gacaaaaatg gctttgataa ggtagaatta
    12601 tactatacct tgggagagat gagctatcga agcgatatga gcgcaccagc cattctcaat
    12661 gtgatcgagt tggccaagaa attctatcca cagggcgttt cagtcttgcc gacgacagcg
    12721 gggacaggac ctatgcatac ggtctttgat gccctagagg taccaatggt tgcattcggt
    12781 ctaggaaatg ccaatagccg agaccacggt ggagatgaaa atgtgcgaat cgctgattat
    12841 tacacccata tcgaattagt agaggagctg attagaagct atgagtagag atattatcaa
    12901 gttagatcag atcgatgtga cttttcacca aaagaagaga accatcacag cggttaagga
    12961 tgtgaccatt cacatccaag aaggggatat ctacggaatc gttggatatt ctggagcagg
    13021 gaaatcaacc cttgtacggg tgattaatct cttgcaaaaa ccatctgcag ggaaaattac
    13081 cattgacgac gatgtgattt ttgacggcaa ggtgaccttg acggcagagc agttgcgtcg
    13141 taaacgtcaa gatatcggga tgattttcca gcattttaac ctgatgagcc aaaagacagc
    13201 agaggagaat gtagcctttg cccttaaaca ctctggactc agcaaggaag aaaagaaggc
    13261 taaagtagct aagttgttgg acttggttgg tttggcagat cgtgctgaaa actacccttc
    13321 acaactatct ggagggcaaa aacagcgtgt ggcaattgcg cgtgccttgg ccaatgatcc
    13381 aaaaatcttg atttcagacg agtcaacttc tgcccttgac cctaagacaa ccaagcagat
    13441 tttggccttg ttgcaagatt tgaaccaaaa attaggattg acagttgtct tgattacgca
    13501 tgaaatgcag attgtcaaag acattgccaa ccgtgtggcg gttatgcagg atgggcattt
    13561 gattgaagag ggaagtgtcc ttgaaatctt ctcaaaccct aaacaacctt tgactcaaga
    13621 ctttatctca acagccacag gtattgacga agccatggtc aaaatcgaga agcaagaaat
    13681 cgtggaacac ttgtctgaaa acagtctctt ggtgcaactc aagtacgctg gagcttcaac
    13741 agacgagcca cttttgaatg aattgtacaa gcattaccaa gtaatggcta atattctcta
    13801 tgggaatatc gaaattctcg atggtactcc tgttggagaa ttggtggtgg tcttgtcagg
    13861 tgaaaaagca gcgttggcag gtgcccaaga agccattcgt caagcaggtg tacaactaaa
    13921 agtattgaag ggagtacagt aagatggaat cattgattca aacctattta ccaaatgtct
    13981 ataaaatggg ttgggctggt caggcaggct ggggaacggc tatctactta actctttata
    14041 tgacagttct ttccttcatt atcggaggct tcttggggct agtggcaggt ctctttctcg
    14101 tcttgacagc gccaggtggt gtcttggaga ataaagtcgt attctggatt ttagacaaaa
    14161 ttacctcaat ttttcgtgcg gttcccttta tcatcctctt ggcaatcttg tcaccacttt
    14221 ctcacttgat tgttaaaaca agtatcgggc caaatgcagc ccttgtccca ctttcttttg
    14281 cagtctttgc cttctttgcc cgtcaggtgc aggttgtctt ggctgaattg gatggcggtg
    14341 tcattgaggc ggctcaagcg agcggagcga ctttctggga catcgtgggt gtttacctat
    14401 cagaaggtct tccagatttg atccgtgtga cgactgtgac cttgatttcc cttgttgggg
    14461 aaacagctat ggccggtgcg gttggagctg gtggtatcgg taacgtagcc atcgcttatg
    14521 gatttaaccg ctacaatcac gatgtgacca tcttggcaac cattattatc attttgatta
    14581 tctttgcaat ccaattcttg ggagatttct tgactaagaa attgagccat aaatgaaaaa
    14641 gagccgcgtg gctcttttta actgatcaga ttttctgtgc aaatttttta ctcaaggctt
    14701 gtccaatcaa ggcacccact agggctccga tgacaatact tgcgataaat agaaggacag
    14761 ttccagggtt tggagcgacc atgatacggt cgatatattc ttgggatttt cctcttgcca
    14821 gaagagtagc catataggct ttgggcgcaa tccacataag caagattggt cctgttgtac
    14881 taaaggcgaa aataatgaaa gaaaggaagt tctttgtttt gtccttgtat tttcctaaat
    14941 gagctacttc atctgctagg aggccacaga taattccagg aaggaaggaa ccagcaccgt
    15001 gtttagttcc caagaaaaag aaggcaatga caaggccgat agtggtaatg gctccaaagc
    15061 gcggaacttt tgcgactagg atcatataga cgctaccgcc gacaagggca gtaaaggcag
    15121 gcgcataaaa catgtttcca gtttggtcaa agagattgcc caaaaggaca ccaatcccca
    15181 tgcagaggaa gtaaagaact gcaaaaagca gtgtagttaa gatagatttc ttcatgaatt
    15241 gtctcctgat aattttttca caattctcat tttaccatgg tttggtggga ttgtaaatgg
    15301 atactagaag tttttgaaaa cgcttgaaat atgatataat ggtttcatag ttatttttag
    15361 gggaatatca tgggtagatt tttagacttt gtctttaatc gtttcttttt agggatgatt
    15421 gcgacagcct tcttttggct attaacttta gcaggaggga ttatccttgg tctagcgccg
    15481 gctagtgcca ccttgatgag cttatatgca gaacatggtt atagctttcg ggaatacagt
    15541 ttgaaggagg cttggtctct ttacaagcaa aattttgtct caagcaacct gattttctat
    15601 agctttttag gtgtgggtct agttttgacc tatggtttgt atctcttggt gcaattgcct
    15661 catcagacca ttgttcattt gattgcgacc cttttgaatg tcctagtagt tgccctgatc
    15721 tttttggctt atacagtatc tttaaaatta caagtttatt ttgccttgtc ctatcgaaat
    15781 agtctcaaat tatccttgat tggcatcttt atgagtctag cagctgtggc taaggttctc
    15841 cttgggactg tgctacttgt agcaattggt tattatatgc ctgccctgct attttttgta
    15901 ggaattggga tgtggcattt ctttatcagt gatatgttgg aacctgtcta tgaaatcatc
    15961 catgaaaaat tggcgacaaa atagaatgaa gcagttttgg ctacatacgc ttctaagaac
    16021 ctatagttca gtgatgatca ttatcattgc gagttttgca atcttactct cttacgctga
    16081 ctgggattca cgtgaaaagg aagcccagag agtagcccag cgtgtaactg ctagaacagt
    16141 gagtgaaatt gaatattatc atagagagtc aacccagata gctcaggctt tagttgaaaa
    16201 tcaagctcgt attgagggaa tctataaata ctttagcctt agcatgccag actattttta
    16261 ctggcaatta gagcggaaag cttcgcctta tatatcagtc tctctgtatg aaaatgttga
    16321 tgacctctat gttcgaaatg attttgtaac tggagtggcc attgcttttc aagattacaa
    16381 ggaagtctat gtttctacta aagacaaacg tagtggagaa aaaatcaggg ctgaggattt
    16441 caaaccagca ggaaatagtt ttgccattcc ggtgtcagat ccagtgtcag atcaagattt
    16501 aggagtgatt tacatctcct tggctcctgc tgttttatac catgccattg ataatactag
    16561 aggtcatact ccgatggcag taacagtgac ctcacctttt gatacggaga tttttcatat
    16621 tggtgagaca gttgataagg agagtgaaaa ttggctagtt ggcttaactt ctcatggata
    16681 tcaggttcag gtggcagttc ctaaaaactt tgttttacaa ggaacagtga ctagctctgc
    16741 tttgattgtg ggtttgagcc ttctctttat tgtcattctt tatctgactt tgaggcagac
    16801 ttttgctaat taccaaaagc aggtagtgga tttagtagaa tccattcaag tcattgctca
    16861 aggcgaagag ggtcgtcgga ttgacatttc cgagaaagat caggaattac tcctaatcgc
    16921 ggagacgacc aatgatatgt tggatcgatt ggaaaagaat atccatgata tttaccagtt
    16981 agagcttagt caaaaagatg ccaatatgcg agccttgcag gcgcaaatca atcctcattt
    17041 tatgtataat acgctggagt tcttgcgcat gtatgcagtt atgcagagtc aagatgagtt
    17101 ggcagatatc atttatgaat tcagtagtct cttgcgtaac aatatttccg acgaaagaga
    17161 gaccctcctc aaacaggaat tagaattttg ccgtaaatac agctatctct gcatggttcg
    17221 ctatcccaag tccattgcct atggtttcaa gatagatcca gagttagaga atatgaagat
    17281 tcctaagttc accttgcaac cgctggtaga aaattatttc gcgcatggtg ttgaccacag
    17341 gcggacagat aatgtgatta gcatcaaggc tcttaaacag gatggttttg tggaaatttt
    17401 ggtggtcgat aatggtagag gaatgtcggc tgaaaagttg gcaaatatcc gagaaaaatt
    17461 aagtcagaga aattttgaac accaagccag ctacagtgat caaaggcagt ctatcgggat
    17521 tgtcaatgta cacgagcgtt ttgtgctcta ttttggagac cgctatgcca ttactataga
    17581 gtctgcagag caagccggtg ttcagtatcg tattacaatt caagatgagt agaaagggag
    17641 aaaatgtata aagtattatt agtagatgat gagtacatgg tgacagaagg tctgaagcgt
    17701 ttgattccct ttgataagtg ggatatggag gtcgtcgcaa cagccagtca tgccgatgaa
    17761 gctctagaat atgttcagga aaatcctgtc gatgtcatca tttccgatgt caatatgcca
    17821 gacaaaacag ggcttgatat gattcgggag atgaaagaga tcttaccaga tgctgcctat
    17881 atcctgctct caggttatca ggagtttgat tatgtaaaaa gagcaatgaa ccttagtgtg
    17941 gtggactatt tggtcaagcc tgttgataag gtagagctgg gaaatctgct ggagaagatt
    18001 gcaggtcagc tcggcgagag agggaagaaa agtcagactc ttagtcaaga attagacgag
    18061 gctggatttg ttagttattt aggggataag gagaattggt ggataggtct atccaaggaa
    18121 aaacaaggtt ccttcaccat tccctactat gtcttgggtc aagactggca gattttcatt
    18181 tctgaccaac ccctagatgg tttagtcgtt acaccttttg aagctcctta tcaagaacac
    18241 tttgaacgct ggaagctgaa tgctgagaaa accctctttt acggctctgt aaatctgcag
    18301 cagtctgaga gtctctttgc ctattacgaa ccgatttata gggttatcat tcagggaaat
    18361 ctcaatcaaa tcgtagaaga gttaaatctc ttggagaagg tagttcttga aaatacgccg
    18421 cgtgttccga ttactaaaca gctttttatt cagtttgtca tggatgtttt ccatttattt
    18481 gaacatctca aagctgatga cctgacagac atcgtcaaaa ccattcatgc tattcaatcc
    18541 ttcgatgaat tggttcctta tatcaaggaa actctgacca gctttttcgg tcaataccgt
    18601 atgaatgaaa atgtggtcag tgtgctggaa gtcattggtc gtgattacca aaaagagctt
    18661 tccctcaagg atatcagtaa ggctctcttt atcaatcctg tctatctagg gcagttgatt
    18721 aagcgtgaaa ccgattcgac ttttgcagag ttactaaaca aacaacgtat taaggctgcc
    18781 caacaacttt tgctttcaac tagtgacagc atcgaagata tttgttatgc tgttggttac
    18841 agtaaccttg gatatttcta taaagttttc cgaaaattgt gcggaaaatc gccaaaagcc
    18901 taccgaaaac aggtagaaac tatactataa aatttgtatt cctttacaaa aggtgctata
    18961 atattatcag gaggttctat catgaaaaag aaaccaattt atctatgggt cttgctaatc
    19021 ttgtctgccc ttatttcagt tccgtctctg tttggaattg tgagtccctt gcctagtaaa
    19081 gaagcccttc gtgctgctca gaaacaagtt gcaggggtca atgctcagca attagaagac
    19141 cagcttaatt atacctatag agtagcagaa gcatctcatt ctatttttaa tgttgctttg
    19201 attgtgctat ctactatttt agtggtcgta gcgattgtct tccttgttcg taaaaatttg
    19261 caatatgcaa actatactta cgttggctat gttttgttag ctattattgg ttcgatttat
    19321 ggctatgttg gtttacaaga cgctgtgcag ttggttcaag atgagaccat gcgtttgaca
    19381 gtgagtattg gttcaaaagc tgttagcatt ttctatatcg tcatcaatgt tctcttccta
    19441 gcccttgtct tctataagat gtggcgacaa cagaaagcct tggcagaaga agaggaaaca
    19501 gaagagcttg cctaagtatt gacaaaaaag aactatcaag atacaatctt ggtagttgtt
    19561 ttttgattat agataaaatc atggaggtac atatgaagac aatttcttta gtttatatta
    19621 gtttgagtgg caacactgaa agttttgtga cgcgcttgaa agactatctc ttgtcccagt
    19681 acaaagggat tgaggttcaa aagattcata tcaaggattt ggtcaaggaa ggcaaaaatt
    19741 tctatgaaat ggaccatccc tatgtcgctt ttttgccgac ctatctcgaa ggtgggaatg
    19801 gcgtggataa cggagatgtt gagattttga cgacaccggt gggagatttt attgcctatg
    19861 gtaacaacgc tagtaagtgt tttggtgtgg ttgggtcagg aaatcgtaac tttaataacc
    19921 aatactgcct aacagccaag caatacagtc aacgctttgg cttccctgta ttggctgact
    19981 ttgaaatgcg aggcatgctg gaagatatca aacatgtcgc agctattatc gcagatttgt
    20041 atgagttgga aaaggagaat taatactcaa tgaaaatcaa agagcaaact aggaagctag
    20101 tcgcaggttg ctcaaagcac agctttgagg ttgcagatag agctgacgtg gtttgaagag
    20161 attctcgaat agtataaagt tcttataaga ggtggttgaa gttgttcaac tgttttttga
    20221 gtataaacag tttttaagaa cgtaaaaaag cataagttca ggtcttcaaa accttgttct
    20281 tatgcttttt atcatagaaa tatttgctga attttctctt gaaatgaaac cttatcttca
    20341 tctcgcgctt gtcccttacc ctgcttttcc gattgcgagt gcgtagataa agaagatggc
    20401 gaagccaaag aggaaaatca atcccgcaat ggcaaggtgt ttgagccaag aaggaagatt
    20461 tttgttttca cgcgttacca aggcgtaatt caaaagagca aagaaaggtg ttgtcaggaa
    20521 agagccaatc atggcaaagc ggagcatggt tgaaacctga ccagcgaaga acttgataat
    20581 gacgataccg atgatagcag tgatggtcat ccagatgttc aaagatttac gattgtcctc
    20641 tttttgactg attagcagtc ggagagattc ctgattaacg cgagaatagc catcgataac
    20701 agttataact gttccaaaga tacagaggaa ggcaataaag gtaatcaagt aacgggacca
    20761 ttcgccaaga acagaggcat acatgcccac gaattgagag atgtatttgg ctgaagcagc
    20821 ttcaaccgcc tgccctgtag gatactgaat cagtgctccc agtgccacaa agaagacggc
    20881 taggatagct gttccaatat aaccagtgtt aaagtcaaac agagcgtctt ctgtgttaaa
    20941 gttgacggtc tttctctttt cagctgacca aagtgaattg atggctgaaa tttcaatagg
    21001 agccggcatc catcctagga gggagacgat gaagggcaga gctgccattt gccaaggtgt
    21061 cttctcgaca aaatcagaac tgtattctgg atgcttgacc gccgcaatga taactgcaag
    21121 aacagtcgca atggttaagg cagacataat ccatttgacc atgccgtcta aaagtttgta
    21181 gcctccaaag agtagcatag cccaaatgat tgcaacgaga atgagggacc actgagtaat
    21241 gctaagtcca atcattggga aggcactggc gatgatagct gagcacagaa tggcaacacc
    21301 agccgtgttg accatagccg aaaagacatt gaggataaag aaaatccaga gatagagttt
    21361 tcctttttcg gcataacctt caaccaaagt ctttccagta tcagctgtgt attcagcacc
    21421 aaaacggaaa aatggatatt taaagacatt ggctaagatg accaagagaa gtagagacca
    21481 accgtaagaa ccgccagctt gagttgagga tacaatgtgg gaacctccaa cagcggcagt
    21541 tgccattagg attccaggtc ccattgcttt tagcttgctt gcccaggttg attgatttaa
    21601 ggaaatagct tgcgacatga taaatactcc tttttgaatt ttcagaataa tctgtatatg
    21661 taatctatat tctacaaaaa attttggaaa aatgcaagag tattttgaaa aaagatagaa
    21721 aatttacaaa gaaaatctga aataactgtt atgatataga ggattgaaac tagaatagta
    21781 cacctctgct tctaaaacat tgttagaaat cgatttgact gtcctgatcg atttatcctg
    21841 ttcttatttc atttcactat agtaaggtgc agcaggataa agaaaaaccg agaagtttct
    21901 ttctcggatt tgcttattat gaggttccta ttttctatgc tccaagagtt gattgatgtg
    21961 atctactttt ttagcctctc ttttatagag aatagcccag atgatgagat agacaatcgc
    22021 aaactcgata atgagctgga ggtagaaaat ccagtggaaa gggaaccaac cagcaagagt
    22081 tgctagtggg acaaagccag ccagcataag gaagaaatga gtcagagtgg cacgaagcat
    22141 gctccagtca cggctaaata agcggttgcc aaagttgaag agcataccga tggttgccca
    22201 gataagtgtg cagtagagca agaccagggc accgtgaacc tgatgctgat ccatcacttg
    22261 gccgatgaga gagtagggat ttagtggtgc gtaggtattt ggtgcataaa tgagtgaaaa
    22321 gaggatagag aggatgaggc cgataagaac accggcagct gcatcgtgaa atacttgttt
    22381 tttcatagtt ctaatttctc cttgatggtt tttagataac ggcgtgaaga gtaggtgaag
    22441 ctttcgtttt tcaagaaaat ttctatcaga ccgtttggcg tgagcttgag gtgagagatg
    22501 gaatcgatat tgatgatttc tgattgggaa atttggataa aattggttgg caagagttta
    22561 agaacctgat agagtcgcaa atcaatgctg taggtctgac tcgcggtttc tgctagaacc
    22621 ttccgattct cgatatagaa gcgctgaatc ttgccaatct caactagata gacctgatca
    22681 tcgattttcc ctttgatttt ttctctttgg tccagatttt ctgcgaactc gatgactttc
    22741 tgaactttat ctgtcggctg aggtgcttgg acaatcagct tttcctcctc gtaagtctca
    22801 ctaatctgta attctacttt cataaatttc tctccttgtt tttcatacaa aattaatctc
    22861 acttcttgta cacctttatt aatactcttc aaaaatctct tcaaaccacg tcagcgtcgt
    22921 cttgccgtag gatagttgtt gactttgtca gttttatcta caacctcaaa acagtgtttt
    22981 gagctgtctg cgcctagctt tctagtttgc tctttgattt ttattgagta taccactatt
    23041 ttactccctc tggcaaggta ctttgtctat gtggagggat tgggctccta tgtggtggag
    23101 cttttctgtt ctttctgaaa tatggtataa tagcactaat caatttctag gaaaatagat
    23161 acagaaaggg gctgaaagaa ctaggggaac agttgtaatt agaaggtgat aggtatgaaa
    23221 agtggcgttg aagctggata agtttataaa gagtttgaat gaagaaatga aaagtaaata
    23281 agttgatgat gaattgcaaa aatagatttt atatacaatt tgtatctttc aaatcattga
    23341 tttgaatttt agatttaatc atgttacaat taaaaatgaa atcaaataga agaggaagat
    23401 gcaaacgtgg gcacactatt agcaacaaga ttaaaaaata gacgaaaaga attaaaaatg
    23461 tctcagcgag aactagctga aggtatctgt aaacaggggc aaatcagtcg attagagagc
    23521 ggagagttta ctcctggagc agatttcttg catgcccttg ctaagaagtt aaaagtcagt
    23581 atggattatt tttttgatga gcaggttgta gagaaagttg aggagttatc agaatttaag
    23641 aaattagcac agacatttat tacaaatcgc aattatgagt ctttgaaata tatatatgaa
    23701 ttggagaatg taaaagctca ccgcctatct ttagcagata aaatctacat ggaatggata
    23761 aaatctctga tagattttta tttttacgga cagaaagaag atgcgattgt acgattagag
    23821 ggggtattat cacagttaag tgtatctgat ttgaactatc tccaagtttc aaacacccta
    23881 ttcaattttt attatgatat tggagattta ggtcgtttca ataaaattcg tgaaaaacta
    23941 gagtatcaag taaatcacct caatttgagt acaatcgaag agttagaact atctattaag
    24001 tttaactaca acgtttgtcg ttatctatgg ttacagaaaa atattgaaga agctattact
    24061 aaaattacag ctactataaa acaatgtaag gcttacagaa cgaattatct tttggctgat
    24121 ttgtacttat tgatgggaaa tgtaagtgaa aatttttctt ctaaaagttc ggtgaaagaa
    24181 tattttgaga cggcatattt tctatacaaa cttgaggaga acatgtcaat gactctgaaa
    24241 gttgagcatt acattgcaga tataacagaa taatgaatac ggtgttcaag tgagcatcgt
    24301 ttttcaaacc tagttcagat acaaaaaagt atcaaacaaa aaaaaataaa aaaacattat
    24361 aaaaacaatt gacaagctaa ttttgaagtg ataaaataaa tgcaagaaaa caaaaggaga
    24421 ggtatatgag aagtttgttt aagaaaatag ttgctgtatt agttattggc ttaattctgt
    24481 taggaattgc gagaacacct caagttcata agatggctaa agggattgat cctggtcctg
    24541 cgaattttat ttagaaatca atttctacta attgagtact aattgagtat taatgggtaa
    24601 aaagattgta tggataagaa aaatttactt gtattggtta ctggttcggt aggtgccagt
    24661 aatgtagata catacttata ttatataaaa aaattctata atgttaaagt gatactttct
    24721 gaaaattcta aaaaatttat aagtaaggaa ttaattagtt atttttgtga taaagtttat
    24781 gatgaaatat ttgttgaaga ggtagttccg cacgtttttc tgccatatga atctgactta
    24841 cttcttattt taccagctac ggcaaatgtg attggcaaaa ttgctaatgg tattgctgat
    24901 gatttagtta cagcaactgt tttaaacttt aataaaaaaa taattttttg tcccaatatg
    24961 aactctacta tgtgggacaa tcacatagtt caaagaaatg tatcaattct aaaggagttg
    25021 ggacatatat ttttatttga gtctaaaaaa acatatgagg taggattgcg taaagcaata
    25081 gattcaacat gttcaatgtt acaaccacag tcgttagtaa aagaacttat caaattagaa
    25141 aatattgtcc ttgaagaggg acattaaaaa ctactgagaa tattaaggag gggaaaaaat
    25201 ggaaaattca tcaatcgatg tagatatgct gttggaagaa ttgacacaag aagcaatggt
    25261 cgttgttgct gttgataagg actgttaatt taaacttatg gcaatatatg aaaggttact
    25321 ggatgtttta aattatgcag gcagtagcct tttattatat acaaatggat aaagtaagga
    25381 taatacaatg attaataaaa aaatacaaca agttgttttg gaatcattac agaatttttt
    25441 gaatgggaac ttcatttcgc cttgtgtagt ctatgatttt ggcttgctgg aaactgtact
    25501 tgatgaattt aaaaatcaaa ttcctgtaac attcaattac caactttttt atgccgttaa
    25561 agcaaattca aatgagaaga tacttgaatt cttagtagat aaaattgatg gagttgatgt
    25621 ggcgtcatta tctgaattag atgtggctaa aaaatttttc ccaccaactc aaatttctgt
    25681 taatggtccc gcattttctt atgaaacttt atataatctg attaaaaaac aatataaagt
    25741 tgatattaac tttttggaac atcttcaaca attttcccca aaagaatctg ttggaataag
    25801 agtaacggag ccagatgaac ttaataatcg tatgagtcga tttggaataa atatttgcag
    25861 tgataattgg actagtaatt tacaaaatcc tttaattaca cgactgcatt ttcattttgg
    25921 agaaaaagat gataaattta ttgttaagtt agataaaata ttatttaagt tacaagaaat
    25981 taataaactt agagaggtta gagaaataaa tcttggaggc ggttttatga aattatttat
    26041 ggaaaatcgt ttgaaagaat tttttctatc acttatggaa atctataaaa agtacgatat
    26101 tgatagtact gtgactacaa taatagaacc aggtagtgca attacttcat tttctgccta
    26161 tatgattact agcccagtta atgttagtga ggtgaatgag cagcaggtta tcacgttaga
    26221 cacatcaata tacaccaata cattatggtt tgttccgcat attattacaa cgttaaattc
    26281 aagtagtaaa gagcgttata gtactattct ctatggtaat acctgttatg aacatgacaa
    26341 gtataaaatg aaagtttcgc ttccaaggtt aactcaaaat agcagtatag tgttttttcc
    26401 tgtaggagct tatataaaaa gcaatcattc aaatttacat cgtaatgatt ttatgcggga
    26461 ggtatatttg tggacaaaaa acttgacata ttagataaag ttaaggaata tttaggaaat
    26521 aaaactactc aaattctgga taatcaatat aaagaatttt tgaaacttaa tgatataagg
    26581 cgagcgtttg gtatttcaga aaaagtatta aacaattctt ttaattttac gagtaaagaa
    26641 tttaatgatt taattaataa cgaaaattat ttattcgaat atgcatgtag aattagagag
    26701 gaatggagaa aaaaatgctt taatcattct tatcgttttc tatgctcacc tataattaca
    26761 gatgattttc ttaacacgaa gacattgaga agtagccaaa ttgaatataa atatgagcga
    26821 tatttatcga aaagttcgat aggcgataga gcggttgatg gctttgtttc cttcaatact
    26881 ttaacagcta atggtatgtc tgctattaaa ctatgtcttg agatattaaa ctctattttc
    26941 ttcaagaaga agattgattt attatattca accggatatt atgaaacaag atttttatta
    27001 aataatcttg ctaaatcagg tattagttgc tatgaggtaa gtaattgtga attggataaa
    27061 gataaatttt ataatgtatt catgatggaa cccaatcgag ccgatttaac attacaaaaa
    27121 actgatttca agatagtaga atattttgtt aagtataaaa ataattcaat aaaagtcgtt
    27181 attttagata tttcatatca aggttctaat tttaaattag tagaattttt agagaaattt
    27241 aaatttgcga atgtaattat ttttgtggta cgatctttga taaaattaga tcaaatggga
    27301 ttagaattga caaatggggg aataatagaa gtgtttattc ctaatcattt gagaaagttg
    27361 aaaaatttta ttgaagagga attcaataaa tttagaaatt ctcacggagc taatctaagc
    27421 ctctatgaat actgtttgct tgataattct ttaaccttaa aaaatgattg gaactattct
    27481 gatttagtta tgaaatttac gagtaatttt tatgctgata taaaagactt gttcatggaa
    27541 aattctgata ttgaaatcat ccatgaagag ggagtacctt ttgtattttt agatttaata
    27601 ggtgaaggta aaaaagaata tgaaatgttt tttcaatggt taaacttctt ttacaaacag
    27661 cttggaatca cattgtatgc tagaaatagt tttgggtttc ggaatctaac agtagagtat
    27721 tttggaatta ttgggacaga aagatatata tttaagattt gtccaggtgt ttataaaggg
    27781 ttaagttatt atttgatgaa atttttatta aaatcttttt caaatgaata tttaaaaact
    27841 actgatgagg ttaatagatg aaaaatttga taaagttgct aataattaga ttgattgtta
    27901 acttagcaga cagtgtattt tatatagtag cattgtggca cgttagcaat aattattctt
    27961 cgagcatgtt cttaggaata tttattgcag taaattatct accggatttg ttactaatct
    28021 tttttggacc agttattgac agagtaaatc cgcaaaaaat tcttataata tcaattttgg
    28081 ttcaattagc agtggctgta atatttttat tattattaaa ccaaatatca ttttgggtga
    28141 taatgagtct agtgtttatt tcagtaatgg ctagctccat aagttacgtg atagaagatg
    28201 tgttgattcc tcaagtggta gaatatgata agattgtatt tgcaaattct ctttttagta
    28261 tttcgtataa agtattagat tctattttta attcattcgc atcattttta caggtggcag
    28321 taggatttat tttattggtt aagatagata taggcatatt tttacttgct ctatttatat
    28381 tgttgttgtt aaaatttaga actagcaatg cgaatataga aaacttctct ttcaaatatt
    28441 acaagagaga agtgttgcaa ggtacaaagt ttattttaaa taataaatta ttatttaaaa
    28501 ccagtatttc tttaacgctt ataaactttt tttattcatt tcagacagta gttgtaccga
    28561 ttttttctat tcgatatttt gatggtccga ttttttatgg tattttttta actattgctg
    28621 gtttgggtgg tatattggga aatatgctag cgccaatcgt aataaaatat ttaaaatcga
    28681 atcaaattgt tggtgtattt ctttttttga acggctcaag ttggttagta gcaattgtta
    28741 taaaagacta tactttatca cttattttat ttttcgtttg ttttatgtct aaaggagtct
    28801 tcaatattat ttttaattcg ttgtaccaac aaatacctcc acatcaactt cttggtaggg
    28861 taaatactac cattgattct attatttctt ttggaatgcc aattggtagt ttagttgcag
    28921 gaacgcttat tgatttgaat attgaattag tgttaattgc tattagcata ccttattttt
    28981 tgttttctta tattttttat acggataatg gattgaaaga atttagtata tattagaaat
    29041 gtttatgttc attcaaaagc ataatgacta taactgaaaa agaaaagtga tatctttaag
    29101 gttgttcttc ttggtggtga gattcgtgag acaacccaag cttttgtcgg aaagattacc
    29161 aatgctttga tggataggat gtactttagc aagatgtttt tagtggtaac ggtatcgtgg
    29221 atggacgtgt aataacctct tctttcgagg agtattttac taaaaaacta gccttggagc
    29281 gttccccaga aacggactta ctcattgact cttcaaagat ttggggagaa gattttgctt
    29341 catctgttcc ttgaaaaaag tcacagcagt catcacagac gatagtactg aacaaaacta
    29401 tgaagagtta gaaatttata cgcaggtgat tgtataaagg atctggaaat agataagaag
    29461 ttgattagta ttgacctagg tggtacaaat attaagatta ctgttctttc aaatgacggt
    29521 gagattgaaa ctttgtggag tattacaaca gatacaagtg agaaaggttc tcaaattata
    29581 tcggacatca tcagttctat taaaaataaa ttgaccgaac ggaatattcc tgatagcgac
    29641 cttcttggaa tcggtatggg aagttgctca tcatactttc cttgtaaatc ataggggcta
    29701 taaactctcc gtctacttgt cctacaacaa ttgaagtctg ctcaaaacgc cgtccgctaa
    29761 tcttttcata gactttctcc cttttaggag cctagctttc tagtttgttc tttgattttt
    29821 attgagtata ccactatttt actccctctg gcaagggact ttgtctatgt ggagggattg
    29881 ggctcctatg tggtggagct tttctgttct ttctgaaata tggtataata gcactaatca
    29941 atttctagga aaatagatac agaaaggggc tgaaagatgt ctcatattat tgaattgcca
    30001 gagatgctgg caaaccagat tgcggctgga gaggtcattg aacgtcctgc cagtgtggtt
    30061 aaagagttgg tagaaaatgc cattgacgcg ggctctagtc agattatcat tgagattgag
    30121 gaagctggtc tcaagaaggt tcaaatcacg gataacggtc atggaattgc ccacgatgag
    30181 gtggagttgg ccctgcgtcg ccatgcgacc agtaagataa aaaatcaagc agatctcttt
    30241 cggattcgga cgcttggttt tcgtggtgaa gccttgcctt ctattgcgtc tgttagtgtc
    30301 ttgactctgt taacggcggt ggatggtgct agtcatggaa ccaagttagt cgcgcgtggg
    30361 ggtgaagttg aggaagtcat cccagcgact agtcctgtgg gaaccaaggt ttgtgtggag
    30421 gatctctttt tcaacacgcc tgcccgtctc aagtatatga agagccagca agcggagttg
    30481 tctcatatca ttgatattgt caaccgtctg ggcttggccc atcctgagat ttcttttagc
    30541 ttgattagtg atggcaagga aatgacgcgg acagcaggga ctggtcaatt gcgccaagca
    30601 atcgcaggga tttacggttt ggtcagtgcc aagaagatga ttgaaattga gaactctgac
    30661 ctagatttcg aaatttcagg ttttgtgtcc ttgcctgagt tgactcgggc taaccgcaat
    30721 tatatcagcc tcttcatcaa tggccgttat attaagaact tcctgctcaa tcgtgctatt
    30781 ttggatggtt ttggaagcaa gcttatggtt ggacgttttc cactggctgt cattcacatc
    30841 catatcgacc cttatctagc ggatgtcaat gtgcatccaa ctaagcaaga ggtgcggatt
    30901 tccaaggaaa aagaactgat gactctggtt tcagaagcta ttgcaaatag tctcaaggaa
    30961 caaaccttga ttccagatgc cttggaaaat cttgccaaat cgaccgtgcg caatcgtgag
    31021 aaggtggagc aaactattct cccactcaaa gaaaatacgc tctactatga gaaaactgag
    31081 ccgtcaagac ctagtcaaac tgaagtagct gattatcagg tagaattgac tgatgaaggg
    31141 caggatttga ccctgtttgc caaggaaacc ttggaccgat tgaccaagcc agcaaaactg
    31201 cattttgcag agagaaagcc tgctaactac gaccagctag accatccaga gttagatctt
    31261 gctagcatcg ataaggctta tgacaaactg gagcgagaag aagcatccag cttcccagag
    31321 ttggagtttt tcggacaaat gcacgggact tatctctttg cccaagggcg agatggactt
    31381 tacatcatag atcagcacgc tgctcaggaa cgggtcaagt acgaggagta ccgtgaaagc
    31441 attggcaatg ttgaccagag ccagcagcaa ctcctagtgc cctatatctt tgaatttcct
    31501 gcggatgatg ccctgcgtct caaggaaaga atgcctctct tagaggaagt gggcgtcttt
    31561 ctagcagagt acggagaaaa tcaatttatt ctacgtgaac atcctatttg gatggcagaa
    31621 gaagagattg aatcaggcat ctatgagatg tgcgacatgc tccttttgac caaggaagtt
    31681 tctatcaaga aataccgagc agagctggct atcatgatgt cttgcaagcg atctatcaag
    31741 gccaatcatc gtattgatga tcattcagct agacaactcc tctatcagct ttctcaatgt
    31801 gacaatccct ataactgtcc tcacggacgt cctgttttgg tgcattttac caagtcggat
    31861 atggaaaaga tgttccgacg tattcaggaa aatcacacca gtctccgtga gttggggaaa
    31921 tattaaaagt ataaaaaagt ctgggaaaaa ttttcaaaat cagaaaaacg cataaaatca
    31981 ggtgttcaaa aaccttgatt ttatgcgttt tatcatagaa atatttactt catgttttcc
    32041 taattctttt cgaaactctt tttaaaccac gtcagtttta tcagtaatct caaaacagtg
    32101 ttttgagcta attttgccag ttttgtctgt aacatcgaag ttgtgtttga ccactctgcg
    32161 actggtttcc tagtttgctc tatgattttc acagagtatt aaattgcgat tttgccaagt
    32221 ttctttattc gtctaaaagt agagtctgtt ctatgcgtct aatgtacgaa ttaggttgac
    32281 catttcaata gctccttgtg cacactcaga acccttattt cctgctttag tgccagctcg
    32341 ttctatggct tgttcaattg tatctgtcgt tagcacacca aacataacag gaatttcgct
    32401 atttaaactg atttgggcga ttcccttaga tacctcgcta catacataat cataatgact
    32461 tgtattccct ctaatgacag ctcccaagca gataattgca tcatattttt tactttttgc
    32521 catttttgat gcaatcagtg gtatttcaaa agctcctgga acccaggcta cctctatatc
    32581 tttctcgttt acattctctc ttttgagatt atctagtgct ccagataata attttgaagt
    32641 tataaattca ttaaatctcg ctacaacaat acctatttta atattgtttg ctactaaatt
    32701 accttcataa gtgttcattt atttttcctc catatttaaa atgtgaccca ttcgattttt
    32761 ctttgtttct aaataaaaac tatcgtaagg attggcttct atttcgattg atattctact
    32821 ggaaatggta attccatatt tttctaactg ttcaaccttg tcaggattat ttgtcagtaa
    32881 atgaagtgac tgaagtccca gatctttaag catttgtgct ccaatatgat attctcttaa
    32941 atcaccttca aagcctaatg caagattggc atcaagcgta tccatgcctt gatcttgtaa
    33001 atgataggct tttaatttat tgataagtcc aattcctcgt ccctcctgtc gcaagtaaag
    33061 taagacaccc gaaccattct caacaatcat tttcatagct ttatcgaatt gctgtccaca
    33121 atcgcaacgt aaggagccta aaacatcccc tgttaaacat tcggagtgga cccgacataa
    33181 tacattggct tcatcctcta tgtttcccat aataagagca agatgatgtt ccccatttag
    33241 tttatctata tagctaattg ctttgaaatt accgtatcta gtaggcatat tgacagttga
    33301 aactcgttct accagctgat catatacttt tctatattct tgtaattctt tgatggtaat
    33361 tagtggaatg ttgtgtttct tcgagaactg aattaaatca tctgttctca tcattttgcc
    33421 atcatgattc attatttcac aacataggcc acactctttt agtccagcta attttaataa
    33481 atcaacagtt gcttctgtgt gtccacttct ttctagaaca ccaccttttt ttgcaattaa
    33541 aggaaacatg tgtcctggcc tgcgaaaatc agagggtgtt atatcttcag ctacacacat
    33601 acgtgcggtc agtcctcttt cctcggcaga aatacctgtg gtcgtttctt tataatcaat
    33661 tgaaactgta aaagcagtct tatgattatc tgtattgttt tcaaccatag gtgaaagcat
    33721 taattgatta gctaaacttt cgctcatagg catacaaatt aatcctttgg cataagtagc
    33781 cataaaatta acattttctg ttgtagctgc ttgtgcagaa caaattaagt ctccttcatt
    33841 ttctctatcc ttgtcgtcta taacaagaac aagtcgtccc ttctgcaatg cttctaatgc
    33901 ttcttgtatt tttcgatatt ccattgactg attatccttt ctgctaaaat ccattttgat
    33961 ataatagttc cttagatatt tctgattttg gagagttatc catcagtttt tgcacatatt
    34021 tacctaagat atcattttca agatttactg tactcccgac ttgtttactc ttaagaatgg
    34081 tttgttccaa ggtatgaggg ataacagata ctgaaaagtt tactttggag actttagcga
    34141 cagtcagact aatgccgtca attgtaatag atcctttttc aactattaaa tctaaaattt
    34201 ctttttgtgt gttgatttga taccatacag cattatcatc tttttttatt gacgagattt
    34261 ttcctgtacc atcaatgtgt cctgtaacga cgtgaccccc aagtcgaccg ttgacagata
    34321 aggctctttc tagattcacc tcacttccat gttttaatag agtaagagct gttcgactcc
    34381 atgtttcatt cattacatca actgtaaagg attgatgatt gaaatgagta actgtaagac
    34441 agataccatt tactgctata ctatcgccta aatggatatc cgttaatatt tttgaggctt
    34501 taattgatag tttacaatta cgagagtctt tctgtattct ttcaactttt ccgatttctt
    34561 caattattcc tgtgaacatg gataaatcac ttcactttct atgagatagt catttcctat
    34621 ttgagaaaat gcataaggtt tcaatctaat agcgtcattt ggcaaagaaa taccttcacc
    34681 tccgacagga aacttggcac tacctccaaa aatttttggt gcaatatata ttttcagctc
    34741 atcaacaatt tgttgttcca aagcactcca attcattaga ctgccccctt ctagaactag
    34801 gctatcaatc tgcatgtttc ctagatgttg cattaaactc gataagtcta tatgattgcc
    34861 ttttttcttt atggaaagta tttcacagcc atgattttga tatagcttca ttttattttt
    34921 gtcttcagag gaagtggcaa tgtaagtttt aatatcattt gctgttttta cgattttaga
    34981 ggtaagagga gttcgtaaat gtgtatcgca tatgatacgg ataggatttt tcccttcctc
    35041 caatctacat gtcagcaaag gatcgtcttg aataacagta ttgactccca ccataattgc
    35101 actaacatgg tgtcgtaact gatgcacatg ctttcttgct tcttcttcag taatccattt
    35161 ggattgattt gttttagtgg ctatttttcc atccattgac attgcatatt tcataaaaac
    35221 atagggtaca tgctgggtaa tatactttct aaaacttttt attaagttaa gacactcatt
    35281 ttctaaaatt ccaacagtaa cttgaagatt attttcctca agtatcttta ctccttttcc
    35341 agatacaata ggattacagt ctaggcttcc aatgactact cttgtaatac cactatcgat
    35401 tatagcatct atacagggag gtgttttccc gaagtgacaa cagggttcaa gtgttacata
    35461 aagcgtcgct ccgacagggg attctctaca gtttttaaga gcatttctct cagcatgtgg
    35521 gccaccaaaa aactcatgat aaccttgtcc gataatgtga ttatctttta caataactgc
    35581 gccgaccata ggattgggat tgacgtaacc agcccctttt tgtgccagtt ttattgctaa
    35641 tttcatatat tttgaatcgc tcatctcact acctccaaaa aaatatgcct tgaatagggg
    35701 actactcaag gcatacaaaa gaaaacttat gcgattaaca aaaatgctct gaaatgacaa
    35761 gtaatcattt cagagcacgc aaaaagcaca aatatacttt tatcttcttt catccagact
    35821 atactgtcgg ctttggaatt tcaccaaatc atgcctttcg gctcgtgggc tataccaccg
    35881 gtagggaatt tcaccctgcc ctgaagatag ttattcaatt acagatgatt atagtactta
    35941 attttgaata tgtcaacaga taaataccga ttgtttttga tatactgtat ttgtgataat
    36001 cgattctcgc tcctcggata aagaaaatat gatatactag ataaacgaaa taagagagaa
    36061 ggaatactat gtacgcatat ttaaaaggaa tcattaccaa aattactgcc aaatacattg
    36121 ttcttgaaac caatggtatt ggttatatcc tgcatgtggc caatccttat gcctattcag
    36181 gtcaggttaa tcaggaggct cagatttatg tgcatcaggt tgtgcgtgag gacgcccatt
    36241 tgctttatgg atttcgctca gaggatgaga aaaagctctt tcttagtctg atttcggtct
    36301 ctgggattgg tcctgtatca gctcttgcta ttatcgctgc tgatgacaat gctggcttgg
    36361 ttcaagccat tgaaaccaag aacatcacct acttgaccaa gttccctaaa attggcaaga
    36421 aaacagccca gcagatggtg ctggacttgg aaggcaaggt agtagttgca ggagatggcc
    36481 ttcctgccaa ggtcgcagtg caagcaagtg ctgaaaacca agaattggaa gaagctatgg
    36541 aagccatgtt ggctctgggc tacaaggcaa cagagctcaa gaaaatcaag aaattctttg
    36601 aaggaacgac agatacagct gagaactata tcaagtcggc ccttaaaatg ttggtcaaat
    36661 aggagcagag aatgacaaaa cgttgttcgt gggtcaagat gaccaacccg ctctacatca
    36721 cctatcatga tgaggagtgg ggccagcccc tccatgatga ccaagcgttg tttgagttgt
    36781 tgtgtatgga aacctatcag gcaggcctgt cttgggaaac ggtactcaac aaacgccaag
    36841 ctttccgaga agcctttcat agctatcaaa ttcactcagt cgcagagatg actgacactg
    36901 aattggaagc catgctggag aatccagcta tcattcgaaa tagagccaag atttttgcta
    36961 cacgcgctaa cgcccaagcc tttctacagt tacaggcaga gtacggctct tttgatgcct
    37021 atctttggtc ttttgttgag gggaaaactg tcgttaacga tgttcctgat tatcgccaag
    37081 ccccagctaa aacaccctta tctgagaaat tagccaaaga tctcaaaaaa cgaggcttca
    37141 agttcacagg cccagtcgcc gtattgtctt ttctacaggc tgcagggcta gttgatgacc
    37201 acgagaatga ttgtgagtgg aaaggtctta aatgatgtct aacaaaaata agggaattct
    37261 gatttttgtg attctctata cagtcctctt tgtgtttgat ggcgttaaat tgctggcttc
    37321 tttaatgcca tctgccattg caaattatct tgtttatgta gttttagctt tatatggctc
    37381 cttcttgttc aaggatagat tgatccaaca atagaaggag attagaaaga ctaaaagaaa
    37441 attcttcttt ggagtcttaa caggatggct ctttctcatt ctgatgactg ttgtctttga
    37501 atttgtatca gagatgttga agcagtttgt gggactagat ggacaaggtc taaatcagtc
    37561 taatattcaa agtacctttc aagaacaacc actactgata gctgtttttg cttgtgtcat
    37621 tggacctctg gtagaagaat tatttttccg tcaggtctta ttgcattact tgcaggaacg
    37681 gttgtcaggt ttactaagca ttattctggt aggacttgtt tttgctctga ctcatatgca
    37741 cagtttggct ctatcagagt ggattggtgc agttggttac ttaggtggag gccttgcctt
    37801 ttctattatt tatgtgaaag aaaaagagaa tatctactat cccctacttg ttcacatgtt
    37861 aagcaacagc ctctccttaa tcattttagc tatcagtata gtaaaatgaa a
//
LOCUS       DAVDWT010000023        37802 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVDWT010000023 DAVDWT010000000
VERSION     DAVDWT010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37802
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.023"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(<1..556)
                     /locus_tag="VUW50_001314"
     CDS             complement(<1..556)
                     /locus_tag="VUW50_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET7983203.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNH"
     gene            1180..1362
                     /locus_tag="VUW50_001315"
     CDS             1180..1362
                     /locus_tag="VUW50_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET7983204.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            1372..1734
                     /locus_tag="VUW50_001316"
     CDS             1372..1734
                     /locus_tag="VUW50_001316"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET7983205.1"
                     /translation="MPNSESTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYGDSAWEHLRAVTGID
                     LLSILTDIANKKTGVKSK"
     gene            1731..2921
                     /locus_tag="VUW50_001317"
     CDS             1731..2921
                     /locus_tag="VUW50_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET7983206.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
                     RDGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSS
                     NLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNEC
                     LNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
                     QQLL"
     gene            complement(2997..4559)
                     /gene="guaA"
                     /locus_tag="VUW50_001318"
     CDS             complement(2997..4559)
                     /gene="guaA"
                     /locus_tag="VUW50_001318"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET7983207.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEEMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            4701..5399
                     /locus_tag="VUW50_001319"
     CDS             4701..5399
                     /locus_tag="VUW50_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7983208.1"
                     /translation="MLPAYIKIHDQIKKDIDEYRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(5442..6338)
                     /locus_tag="VUW50_001320"
     CDS             complement(5442..6338)
                     /locus_tag="VUW50_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET7983209.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKGLFLEK"
     gene            complement(6347..7282)
                     /locus_tag="VUW50_001321"
     CDS             complement(6347..7282)
                     /locus_tag="VUW50_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HET7983210.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSAPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLSQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(7279..8004)
                     /locus_tag="VUW50_001322"
     CDS             complement(7279..8004)
                     /locus_tag="VUW50_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HET7983211.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFEFTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(8088..8723)
                     /locus_tag="VUW50_001323"
     CDS             complement(8088..8723)
                     /locus_tag="VUW50_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HET7983212.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEKYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFNCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(8725..9552)
                     /locus_tag="VUW50_001324"
     CDS             complement(8725..9552)
                     /locus_tag="VUW50_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET7983213.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAKTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINTLFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(9574..10500)
                     /locus_tag="VUW50_001325"
                     /pseudo
     CDS             complement(9574..10500)
                     /locus_tag="VUW50_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809127.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            11151..11762
                     /locus_tag="VUW50_001326"
     CDS             11151..11762
                     /locus_tag="VUW50_001326"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET7983214.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            11807..12535
                     /gene="rlmB"
                     /locus_tag="VUW50_001327"
     CDS             11807..12535
                     /gene="rlmB"
                     /locus_tag="VUW50_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET7983215.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEKNPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFLNRL"
     gene            complement(12574..13485)
                     /gene="trxB"
                     /locus_tag="VUW50_001328"
     CDS             complement(12574..13485)
                     /gene="trxB"
                     /locus_tag="VUW50_001328"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET7983216.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQERAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(13551..13775)
                     /locus_tag="VUW50_001329"
     CDS             complement(13551..13775)
                     /locus_tag="VUW50_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HET7983217.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(13853..14596)
                     /locus_tag="VUW50_001330"
     CDS             complement(13853..14596)
                     /locus_tag="VUW50_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET7983218.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(14596..15396)
                     /locus_tag="VUW50_001331"
     CDS             complement(14596..15396)
                     /locus_tag="VUW50_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET7983219.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(15554..15910)
                     /locus_tag="VUW50_001332"
     CDS             complement(15554..15910)
                     /locus_tag="VUW50_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET7983220.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNREAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(15904..16434)
                     /locus_tag="VUW50_001333"
     CDS             complement(15904..16434)
                     /locus_tag="VUW50_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET7983221.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGEPQDLSNLLLAPIGTNFEKRVWSYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNNRRRSTC"
     gene            complement(16434..16928)
                     /locus_tag="VUW50_001334"
     CDS             complement(16434..16928)
                     /locus_tag="VUW50_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7983222.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            17063..17509
                     /locus_tag="VUW50_001335"
     CDS             17063..17509
                     /locus_tag="VUW50_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HET7983223.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            17506..18153
                     /locus_tag="VUW50_001336"
     CDS             17506..18153
                     /locus_tag="VUW50_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET7983224.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     NQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASVLQYIAIIYYM"
     gene            complement(18505..19821)
                     /locus_tag="VUW50_001337"
     CDS             complement(18505..19821)
                     /locus_tag="VUW50_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7983225.1"
                     /translation="MLKLIIYQFQYSKRQWLGTIPLLFVSSLIVGTSLFGIASSIKTA
                     NINASQLFQMLIIFGGTTLFFLISNNIRLLIDIFKKDYQLWTILGASRTQLSLLVSGQ
                     FYLMAVIVSSIGTILSFIMADSYYKFLQNLLGRDELPDLVITANIQSILLSIFIVPTI
                     VGIGAYFYSSRILKISSILKPKKKKRKVTVTGFVNISVRLFLWLLCIGSIVSAGFIRN
                     KEIIEKQSSIILFLLIIHILIIQSLSPSIQMFLIKFLMRIFPTENYVINTGFWNLLSN
                     PSYLKSIQTSMSMGVTLISGFILYTQNMYSFMNTANGVNEARASFIAYMSAPIILIIT
                     SSISLTILSSNKDIEDIKQLKTLGVSRLQLFKIRIGEAIIHSVLILLVSVIFNLIILI
                     LVSIIGQFLGRSLVDISGFWQPSLIVISLLVIFYSITKGFYLFQDR"
     gene            complement(19839..20570)
                     /locus_tag="VUW50_001338"
     CDS             complement(19839..20570)
                     /locus_tag="VUW50_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7983226.1"
                     /translation="MVNNVAVKVSNLSKEFLLGQDKTVSILKDISLSVNYGEFVSILG
                     VSGSGKSTLLSCLSSLSEPTSGEVVINGVNPYTLKEGKLAKFRRQDIAIIFQNYNLVP
                     ALPVLENVTLPLRLSGKSVDSNKVKKMLDSLNFKAELSSLVTTLSGGEQQKVAITRAI
                     IADSKIIFADEPTGALDSVSRKLIFETLRNLASQGKCVLMVTHDIELASKTDRALILK
                     DGKISRQIIKPSADELYQALESSKD"
     gene            complement(21127..21711)
                     /gene="pdxT"
                     /locus_tag="VUW50_001339"
     CDS             complement(21127..21711)
                     /gene="pdxT"
                     /locus_tag="VUW50_001339"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET7983227.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFIEMCKEKRQ"
     gene            complement(21712..22587)
                     /gene="pdxS"
                     /locus_tag="VUW50_001340"
     CDS             complement(21712..22587)
                     /gene="pdxS"
                     /locus_tag="VUW50_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET7983228.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(22739..24118)
                     /locus_tag="VUW50_001341"
     CDS             complement(22739..24118)
                     /locus_tag="VUW50_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7983229.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            24412..25335
                     /locus_tag="VUW50_001342"
     CDS             24412..25335
                     /locus_tag="VUW50_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET7983230.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGISPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     PDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVNGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            25395..26000
                     /locus_tag="VUW50_001343"
     CDS             25395..26000
                     /locus_tag="VUW50_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET7983231.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLNAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            26019..27263
                     /locus_tag="VUW50_001344"
     CDS             26019..27263
                     /locus_tag="VUW50_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET7983232.1"
                     /translation="MKFVGVVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(27486..27743)
                     /locus_tag="VUW50_001345"
     CDS             complement(27486..27743)
                     /locus_tag="VUW50_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HET7983233.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEKGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(27785..29821)
                     /locus_tag="VUW50_001346"
     CDS             complement(27785..29821)
                     /locus_tag="VUW50_001346"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET7983234.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEILVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLILSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(30033..30950)
                     /gene="glyQ"
                     /locus_tag="VUW50_001347"
     CDS             complement(30033..30950)
                     /gene="glyQ"
                     /locus_tag="VUW50_001347"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET7983235.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISNQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            complement(31145..31906)
                     /locus_tag="VUW50_001348"
     CDS             complement(31145..31906)
                     /locus_tag="VUW50_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET7983236.1"
                     /translation="MENLYLVKDDSQLAAFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSNFNAATQIIRESAVPAYTNNRRMVMTPDLAVWKELYLYQLMDYECSEQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWLSFLIVDKLVENLG
                     SVQAVFDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(32179..33021)
                     /locus_tag="VUW50_001349"
     CDS             complement(32179..33021)
                     /locus_tag="VUW50_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7983237.1"
                     /translation="MDTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREELFVTTKLWNSQQTYEQARQAFEESLEKLGLDYLD
                     LYLIHWPNPKPLRENDQWKIRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     KILPAVNQIRLAPGVYQEEVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTVSRIQANLDCFGIELSHEEHETLKTIAVHSGAPRVD
                     DMDF"
     gene            complement(33097..33174)
                     /locus_tag="VUW50_001350"
                     /pseudo
     CDS             complement(33097..33174)
                     /locus_tag="VUW50_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(33277..34668)
                     /locus_tag="VUW50_001351"
     CDS             complement(33277..34668)
                     /locus_tag="VUW50_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7983238.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEAAILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(34903..35280)
                     /locus_tag="VUW50_001352"
                     /pseudo
     CDS             complement(34903..35280)
                     /locus_tag="VUW50_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            35513..36490
                     /locus_tag="VUW50_001353"
     CDS             35513..36490
                     /locus_tag="VUW50_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7983239.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDTGSVHELLYTMRQTAGIRFEAEK"
     gene            36487..37569
                     /locus_tag="VUW50_001354"
     CDS             36487..37569
                     /locus_tag="VUW50_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7983240.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(37633..>37802)
                     /locus_tag="VUW50_001355"
     CDS             complement(37633..>37802)
                     /locus_tag="VUW50_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET7983241.1"
                     /translation="EQVWKEIRKRGFKNKAFRTLEDVMNQLQDVIQGLEKEVIKSIVN
                     RRWNRMLFESR"
BASE COUNT        11635 a         8119 c         6617 g        11431 t
ORIGIN      
        1 tgtggtttga aagagtacaa cgtccagagt atgaaataca catagctcca tggacaaagg
       61 cttcaatttc aacatctgta cgtttgcgga tctcagctaa ttcttccatt gaaacctcac
      121 gcgctaaaac gacacgagtc aagcctagct ctttccagaa ctcaagggtt tcatagttag
      181 tggcactggc ttgggtagaa aggtggattt caaggcctgg tgcttcagtc gctgcaatca
      241 taatcaaggc tgggtcagat acgataactg ctgcaatccc gatatcacgc agtttacgga
      301 accactcacc agcaccagct tcatttcctt cgtgcataac catattagcc gctacataga
      361 ccttggcacc atacttggcc gcaaactgca cgccttcttc catctgttcg aaagtaaagt
      421 ttcccgcacg gctacgaaga ccataggcct gaccaccgat aaagacagca tctgctccat
      481 actgaacagc tacctttagc ttctctaaag tccctgcagg tgataaaacc tcaggacgtt
      541 ttaatgtttt tgtcatgatt tctcctattt gcaatataaa agtttgacat ttttccttct
      601 cattttatag taaataaagg ataaaatcaa gcctagacgg attgttttga caattctaat
      661 cttttagact ttgcataata actgttaaac agtcgaaaat taccccagtt ttaacaagta
      721 gtcaaaaagt ctaaaaaagt aaaaaccagt atcccaagat actgactcgt aaaaatacat
      781 aaattagtcc ttagatgact actcccactc tgttgctcct ataatggaaa aggattgata
      841 cagtctagaa tatcgctttt ttatttatta ttctgatata aaactgttag aattgatttt
      901 ttgttttata cccaaatatg aatagagacg tagctaataa gttgaataac atatttggaa
      961 aaatgagaaa tagcaatatt actagaactt ttagaaacaa tcaatattta aaaagaaaaa
     1021 agctcaaaga atttattaaa ccatgttata atagatatag ctataatttt atttataaca
     1081 agaggataga aatgaccgaa ttagaaagaa aaaatcgaaa aattagctaa gaaatattct
     1141 gataacttaa acatcaaagt tcaagagaga gttcgtgaaa tggcaaatga taataagagc
     1201 cattatttga tatacagagt tttaggtatt tcatttgaag aaggagaaaa tatcgatttg
     1261 tatcaaaata aaggtcgttt tttatacaaa tatgctggtt catttttaga agaagctgca
     1321 gtactatcct ttaacgaaaa atttggtaca gaaaatactt aaaaagttaa cattcctaat
     1381 tctgaaagta caaaacctaa gacttttgaa attgattgtt tagtcggaga aaaacacgca
     1441 tacgaaataa aatggtggga tgcaactaca gatggagacc atataactaa agaacacact
     1501 agaataaaag ttattcataa caaaggatat ataccaattc ggttaatgtt ctactatcca
     1561 aatagaactc aagctataaa aattcagcaa actttagaaa cattgtataa cggtattgga
     1621 gggaaatatt atggagattc tgcctgggaa catttaagag cagtgaccgg tattgattta
     1681 cttagtattc taacagatat tgcaaataaa aaaacagggg taaaatcaaa atgacagtat
     1741 taaaaggaga taacttagaa atattaaaaa ctattgaatc ctcaagtatt gatttaatct
     1801 atatggaccc tcctttcttt acacagaaaa cccaaaaatt atctaataac aaaaatatta
     1861 tgtattcatt cgaagatacg tggacttcga ttgaggatta caaagaattt ttgtctgtaa
     1921 gattagaaga atgcaaaaga gtgctaaaaa atagtggcag tattttcgtt cattgtgata
     1981 aaattgcaaa tcatcatatt agattaattt tagataatat ctttggagca gatatgtttc
     2041 aaagcgaaat tatatggaac tataagcggt ggtctaattc aaaaaaggga ttattgaaca
     2101 atcatcaaaa catttacttt tattcaaagt caaaagattt taaatttaat acaattttta
     2161 cagagtattc ttctactaca aatatcgacc aaatactagt ggaacgaaaa cgagatggaa
     2221 actctaaaac tatatataaa gttgataata atggtaacta tattctagca aaagagaaaa
     2281 atggagttcc cctttcagat gtttggaata taccatttct taatccaaaa gctaaagaaa
     2341 gagtaggtta tcctacacaa aaacctattc tgttattaga acaaattata aagattgcta
     2401 ctgataaaaa tgatatagtt ttagacccgt tctgtggaag tggaactact ttagtagcct
     2461 ccaagatttt gaatagaaat tatatgggga ttgatttatc tgaggaagct atcaatataa
     2521 ctcagcaacg tctggaaaat gttataaaaa caagttcaaa tttattgaat aaaggaatcg
     2581 aagcatatag aaccaaaact gaggaagagg aaaacattct taaattatta caggcaaaaa
     2641 ttgttcaaag aaataaagga attgatggtt ttttacctaa acattttcaa aaaaaaccga
     2701 tacctataaa aattcaaaaa aataatgaat gtctgaatga gagtatctct ttattacaga
     2761 atgctataaa ctccaaaaaa cttgattttg gagtagttat aaaaactcat tcagataatt
     2821 tattatttga ttttgatacg atccctgaaa atataatagt agttgaccat tttgaactta
     2881 caatcgaaaa atggttaagt aaaagtcaac agttactata gtttgcaaat tatttacaga
     2941 aagaacaaaa aatgctctga aacactgatt tcaaggcatt ttttgttagt ttaaaattac
     3001 tcccactcaa ctgttgcagg tggtttactt gtaatatcgt agacgatacg gttaacatga
     3061 tccacttcat ttacgatacg tactgagatt ttttgaagta cttcccatgg aattttggca
     3121 aaatcagcag tcataccatc gatagaagtg atagcacgga ttgcaatcgt gtagtcatac
     3181 gtacgaccgt cacccataac accgactgaa cgaacgcctg tgttaacagt gaagtattgc
     3241 caaatatcgc ggtcaagtcc agctttagcg atttcttcac gaagaatagc gtctgattca
     3301 cgaacggttt caagtttctc ttcagtgatt tcacccatga cacggatagc aagtcctggt
     3361 cctgggaatg gttggcgcca tacgatatgg tctggcatac caagctctgt accaagagca
     3421 cgaacttcat ccttgtaaag agtattgagt ggttcaatca attcaaactg catctcttct
     3481 ggaagaccac ccacgttgtg gtgtgacttg atagtttgag ctgtatccgt accagactcg
     3541 ataacatctg tatataaagt accttgagca aggaatttca catctttgag cttgcttgct
     3601 tcgtcatcga atacatagac aaactcgtta ccgatgattt tacgtttttg ttcaggatca
     3661 gaaacgccag caagtttgtc aaggaaacgt ttagcagcgt ctgctttgac gatattcaaa
     3721 ccaaacttac caccgagcat gtccataact tgatcagctt cgcctttacg aagaaggccg
     3781 tggtctacga agatacagat caattgatcg ccaatcgctt tttggagaag aaccccaacg
     3841 acagatgagt caacaccacc tgataggcca agaaggacac gtttatcacc gacggtttca
     3901 cgaatttttt tgatctgcat gtcaatgaaa ttatccattg accagtcacc tttagcctta
     3961 caaatgttaa gggcaaagtt acgaaggata tcatttccgt atacagaatg acgaacttct
     4021 gggtggaatt ggataccgta aatgtgttta tctgggtttt cgatggctgc gtatgggcag
     4081 tcagctgatg tacctgtacg aacaaagtca gcaggaatct cagtaaccgc atcaccatgg
     4141 ctcatcaaaa cagtctgttc atcaggtgtt gattcaaaaa gcgctgatgg tgtgtgagtt
     4201 agggttgatt gaccgtattc acgatttcca gcatcacctg caggaacaac ttttcctcca
     4261 agtttatggg tcaataactg cataccataa cagattccca aaattggaat tccgagttcg
     4321 aagatttctg ggtcaatatc aaatgaacca tcttcatata cagaatttgg accacctgat
     4381 agaataattc ctacaggatt gacttcacga acttcagcag ctgaaatttt atggcttttt
     4441 agttctgaaa aaacaccaat ctcacggata cggcgtgaaa tcagctggtt gtactggcta
     4501 ccatagtcca atacgatgat tttttctaca tcttgcaaat cagttgaaat gttgctcatc
     4561 tttttccttt ctcaaaagaa atatcttttc ccaatattct atcacaaata agggcgaatt
     4621 accaactaaa caaggcgaag tttgactttt tctagtttat tggggtacaa tagagaaaag
     4681 atagaaatga agtgaaaaat atgctacctg cttatataaa aatacatgat cagataaaaa
     4741 aggatattga cgagtaccgt tgggctattg gtgagaggct tcccagtgaa agagatttag
     4801 ctgagcagtt tgcggtcagt cgcatgaccc tccgccaagc cgtatctcta ttagtcgaag
     4861 aaggcgtctt agagcgccgt gtaggaagcg gcacctttgt ttccagtact cgagtacaag
     4921 aaaagatgcg agggacaacc agttttactg aaattgtcaa atcccaaggt aaagttccct
     4981 ctagccagct catttcctac aaaaaaacca ttcccaatga gcaagaagtt gccaagctag
     5041 gaatttttcc aacagacaat attatccgaa tggagcgggt ccgctatgcc gaccaagttc
     5101 ccctagttta tgaagttgct tctattcctg aaaaattcat taaggacttt aaaaaagaag
     5161 aaatcaccag tcatttcttc caaaccttgc aaaaacatgg ctatcgtatc ggcaaatctc
     5221 aacagaccat ctatgctcgc cttgctaaag aaaagattgc ccactatttg gaagttgaaa
     5281 aaggacatgc tattcttgga ttgacccagg tttcctacct agaagatggt actgcttttg
     5341 aatacgtaaa aagtcaatat gtaggcgaac gctttgaatt ttatcttgaa aataattaga
     5401 ttaccacgca aaaactctct gtcacggaca aagagtcttt tttatttttc taagaataaa
     5461 cctttcaaag aaatcaaagt tacagcaaac aatatcattg ccataccaag aaaatcaatg
     5521 ggataaaatt gttcattcat tattaagaag gcaaagaaaa tcgccgatat tggctcaatt
     5581 gaagccaaca agcttgactt gaccggtcct atcagactgg ctcctttaag gaaagctgta
     5641 taggcaaaga cagtcccgat aaggataatg cctgcaaacg caaggagaaa atcaagacta
     5701 gtcgggatat cggcctgtag aacccctgta aaaggaaggg cgaccaaacc tgctatgacc
     5761 attcccacac caatgaccaa gctgctcccc cactttttaa tcaaggctat gggtaaaatg
     5821 atatacagag cataagtcaa ggcagaaaag agaccccaga acagaccagc aggtgtcatg
     5881 gataactggt ccaactgccc atgtgttgcg atcaggaagg ttcctccgat ggcgaatatg
     5941 atggaaacta tctctcccag tgtcggtgcc accctatcct tgatacagct ataaattaaa
     6001 attccgacag gacaaacata ctgaagcacc gtcgctgttc ccgcattggt ctcctgaata
     6061 gcagacagat aggcgaattg gttgagaaaa agaccaatca gagcaaaaat aagaagagac
     6121 agcaaactct ttctatcctt taaaaagacc agtattttat cctttgcagt agcataagcc
     6181 aagagcatga gaattccacc agcgattaaa agacgcaagt tggtcaagac cagagccgaa
     6241 attccgtgtg ccattaggta ttggccactc gttcctgaca acccccaagc aataccagcc
     6301 acaactgtta gtaaagtccc ttttaaacta tttgacatgt ctctccttac tcttctttgc
     6361 gaatcaagtt catcacttga ttgcggtcta atatccattg agctctctcg tccttttcat
     6421 aggtacgatt cgataggaaa atagtggctt cttgcttctg acgattccac atgataaagg
     6481 tacctgtata gcccgtatgg tctagccaat ctccttccaa attccatgct aaagaacgtt
     6541 ccttgtcatc caaaggagaa aaattttgac ttaagtctct tgcaaaatca tctgctaaat
     6601 agtgttctaa aaagatttgt aaatccttta tagtcgaaaa taaaccagca ctcccagcat
     6661 gtctacccag gagacgagcc ttgggatcat gcactatgcc tgcctctaca cctctaactg
     6721 ttggaacagc aagctcaact ggcccaaact tagtttccgt cattccccaa ggtttccaga
     6781 cttgatcctt taaaatcaca tccaaatctt gattaaaaat tctttccaaa ataaagccca
     6841 acagcaaaaa atggacatcc gaataaagaa aggctggctg acttcgtctg ttgagatgaa
     6901 acatcgcttc ctttaattca ggggctgtta aaagatcacg attaggaata aaaggatcaa
     6961 ggtctgttgc atgagtcaag agctggcgaa tagtgatgtc tggataatca ctctcaggta
     7021 aaaaatctat taccagtcta tcaatatcta attgacctat ttcccacaag aaggtacaaa
     7081 ctgtgccaac cccaacaacc ttgctgacac tagctaggtc ataaactagt cctgcctcag
     7141 tctgcaagcc atgctctggg gcactctggc ctaaatagaa ctctgtccat tgattgtcct
     7201 taaaatacgc aaaagaggct ccgggataaa ttcctgcctc gatttgttct tctatttttt
     7261 taataatctt ggtccacttc atacttcttc aaaccacaat tcaacattat ttctatcttt
     7321 accaaggaag aatttttcag acttaggaat aaaatattca gtaaactcaa acttctggcg
     7381 aagacttgct atgtctaatt cattgaccaa gaacttgagc atagataagt cccaggtaac
     7441 cgtattgtcc acagtcaaat cctgcccctg agctggaata aaatcaaggg atgccccaat
     7501 ttcagatgat tccaagaaac tttcgatatc agttgggaga tgtaattcca tagaaatctc
     7561 aaatttactt aaagaaattg atgccaaatc tgtttgaaat tcaggttttt ctcctacttc
     7621 tactagactt gctctgtcat cttccgcatg aatcaaaatc aaatcatctt ctggtgagaa
     7681 aatttcaaaa gcgtagccat tttgaccttt atataatcga tgaatcgaat ctgttttaga
     7741 taagattcct tcaatttcta agggattttc caccttgaca atcaatctag ctagtttttt
     7801 tcttccctct accttacgag tacgcatact gggagcttct tctaaaacca gcttttcaag
     7861 acccgtttgg tcacctagtg acagaaaggc cgattcttct aacaaggcct tcattccaag
     7921 ggtttcaata taaaatgttt catttaattt tctattatta gcttttaaag taggaataat
     7981 ccgtacaatc tgatttacat tcataaattc ctccgtcact ttttatttta aaggatttta
     8041 gaatttttta caagatatta tgctcaaatt taacattatt tagctattta gtaaagatag
     8101 tctttcaagg attttgacaa agcctgataa ccagcaatac tgaggtgcag tccatcagtt
     8161 gtatattctt ttttgagttg gcctgcttgg tctgtcaaac aattaaatac tggcacaaat
     8221 tccacctgca tataggcaga tgcaagctct tgataggctt gattccaatt ctgaattttt
     8281 tcattcgagc ggatatagac tgcctgctgg tacttctctc cctcattgac aggcaaaatg
     8341 gaaagcaatt taatctctgt caatggataa tcgcgagcaa cggattgaat gatagcttcg
     8401 agattattga gagcctcatt cacaggaaca tcctttccga tatcatttgt cccaatcaga
     8461 agaaaaattt tatctactgc tccaccatat agatgagcat caaggttctc tagtaacagt
     8521 cctgtctgat agccacgaat tcctcgattg acaatcgtct ttgaagtccc aaatagctcc
     8581 tgtagaggat aatactcgac aatggaatcc ccaataaaaa gaatgtttgg ttctacaaca
     8641 gaaatgtgat ttaggtgacg atatttggtt tgaatttttt cttgttcctt taggagccaa
     8701 ttttctaata actgtactgc cacttcattc tcctttttct aaccagtttt ccaagacttg
     8761 ataaactcct ccttggctgt tggctggtgc tagagcagtc gccacagctt tggctttctc
     8821 atcagcattt tccatcgcat aggcaattcc agccatttca agcatttcaa catcattttc
     8881 actatcacca aaagccatga tttcttggga tttcaagtcc cagcgcttga gtaattcctc
     8941 caagccccat gctttatgaa tcccagcttg gaggatatca atgcaaccat agccactgga
     9001 tacagctcgg acacggccat caaagagagt attgatttct tccaaaaccg aactcaaacg
     9061 ttcctcacca acaaccatgc tcatcttgag cacaccacca aagaggtcag atgttaattc
     9121 atccacaaat tgcatccgtt ggtagaattt ttcaatcatt tctggagtca taaaactttc
     9181 aagatctgta aaaatcgtac cttccttgac aaaatcaccc ttcatccccg ttacaacaaa
     9241 ctggtcctga cacgctcgac ccttgaaatg agccaaagcc ttgttgacaa tggcgtcatc
     9301 ccatgtctta gcctgaatca attcattgtt ttcaaaaata cgagcgccat tagcaacaac
     9361 cagaaccact cgatccacca agggactcag tagttgtctc atgcggtgaa tttcattgcc
     9421 cgtcgcaatg acaaaacgaa tgcccctttg atccaactga tctaaaatct tttccaatcg
     9481 tgggagatca agctgacctc tagcatccag caaggtccca tccatatctg tcgcaattac
     9541 cttaatcatc atttactttc ctctggattc aagctaatac cacttcaatc ccacatgttc
     9601 attcatttct ttataggctg tatcaattcg ttccttagtc gcttcatcta actggtcacg
     9661 atgactgcca tcctctataa aatcttccaa aatataagtt tggtaatgat acaagggata
     9721 tccttctggt gaaaaggttc cctttggtgt tcgattgacc caagtaggat gagctttagc
     9781 tgttccgata gttgtttttc catccttctt cttgatggtg acatccatga gaacaccacg
     9841 ttcagtccac ttagcattct cttcatctcc catagattca attcgttgat tggaaatgaa
     9901 gttccccatt taatagacaa tgagcttctt atctccatct ttttcaatcg tttcagatgg
     9961 ttcaacaacg taaagatgcc ctccaaagat aatatccgct ccccaatcga tcatcttgtg
    10021 ataaagagct ttttgttctt cagttggttc caatcgatac tcaacaccca tctgaggcat
    10081 gataatggtg atatctgctt ccttctctgc ccgttcaatt tcagccttca tcttatcttc
    10141 gtttaaatct gaaagataac gattatagtc ttcctgagaa atatactgct caattccatt
    10201 gaaaccatag gaataggcta acaatgcaac cttgatacca ttcacttcct taatgaccag
    10261 cggagcctga acacgtggtt cgtgcgtata aactccgatt ggagtgattc cagctttctc
    10321 aataatatcg gccgttgaaa taactccctc aacttgcgaa tccaaaatat gattatgagc
    10381 taaatccagc acatgataac ctgcctcctt aatagcatcc ataacttcag caggagcatt
    10441 aaagagaaga taacccgcta aataatgatc cttattaatg attccttcaa aatcaccaat
    10501 agctaaatct gctgcttagt ctccttgctt gagccaagga gtcacatact caaaattttc
    10561 atgaaagtca taggtaccgt cttctttttt agctgaaaag aaaagtccat cgtggtagag
    10621 caaatccccg tgggccataa ttctggcagt tttttcctct tcctactcct gagacttttg
    10681 cttagtcccc tcttgagaaa tagtatctcg tttttgacta gtcaagggat tccttggaag
    10741 ctttcaaaca acaagaccaa gcccattgat aaatcaactg ctagcaagag tatcgccaca
    10801 aatcccttat tgctccactt gcgataactc ctaaaaagtt taccaagccc ttcataaaac
    10861 gaaaagctaa accaccctga tttcgatttt gtcttctttg tatcttcatt ctccctactt
    10921 tcttatgtaa gccttttctt tttattatat cacagataag tatttctttc acaattgaat
    10981 tgaacttccc atctattttc tataaatcct aaatgccata atgctttcaa ttcttgtcat
    11041 tttgtgatat catgtagagg aaatgaacta atccacagtg gcttattcca agtataccac
    11101 ttgggctttg gcagtagcta actgcactaa acataatata aggagagaaa atgtctgcaa
    11161 tagaacgtat tacaaaagct gctcacttaa ttgatatgaa cgatattatc cgtgaaggga
    11221 atcctactct acgcgcgatt gctgaggaag tcactttccc cctatctgac caggaaatca
    11281 tcctaggcga aaagatgatg caattcctta aacattccca agatcctgtc atggctgaaa
    11341 aaatgggact ccgcggtggt gttggactgg ctgctcccca gttagatatc tcaaaacgca
    11401 ttatcgctgt tttggtacct aatattgttg aagaaggcga aactccacag gaagcctacg
    11461 atttggaagc cattatgtac aatccaaaaa tcgtctctca ctctgttcaa gatgctgctc
    11521 ttggcgaagg agaaggttgc ctgtctgttg accgtaacgt gcctggctat gttgttcgcc
    11581 atgcccgcgt tactgttgac tactttgaca aagatggaga aaaacaccgt atcaaactca
    11641 aaggctacaa ctccattgtt gttcagcatg aaattgacca cattaacggt atcatgtttt
    11701 acgatcgcat caatgaaaaa gacccatttg cagttaaaga tggtttactg attcttgaat
    11761 aaagaaaatc ccgttgcaag acggggtttt gtgttataat agaggcatga aaacaaatga
    11821 tattgtctat ggcgtccacg ccgttaccga agccctcctt gcaaatacag gaaacaaact
    11881 ctacctccaa gaagatctcc gaggtaagaa tgttgagaaa gtcaaggaac tagctacaga
    11941 aaagaaggtg tccatttctt ggacatcaaa aaaatctctc tctgagatga ctgaaggtgc
    12001 tgttcatcaa ggttttgttc tacgagtgtc tgaatttgcc tatagcgagc tagattacat
    12061 ccttgcaaaa acacgccaag aaaaaaatcc acttctattg attctagatg gtctaaccga
    12121 tccccataat ctgggttcta tcttgcgaac agccgatgcg accaatgttt caggtgtcat
    12181 cattcccaag caccgtgctg tcggagtaac tcctgtcgtt gccaaaacag ccacaggtgc
    12241 tattgaacac gttccaattg cccgagtgac caacctcagt caaaccttag ataaactcaa
    12301 ggatgaaggc ttctggacct ttggaacgga tatgaacggt actccttgcc acaagtggaa
    12361 tacaaaaggg aaaatcgccc tcatcatcgg aaatgaagga aaaggcatct ctagcaacat
    12421 caaaaaacag gtcgatgaaa tgattaccat tccgatgaat ggacatgttc aaagcctcaa
    12481 tgccagtgtt gctgcagcca ttctcatgta cgaagttttt ctaaatagac tataaaaaag
    12541 tttccagtca tctgattgga aactttttta tgattaacta tgttctgtga taaacttgta
    12601 ggcttcttga ccagcgatag ctccatctcc aactgctgtt gttacttggc gaaggtcttt
    12661 caagcgaaca tctccaactg caaagatacc gtcgactgca gttttcatgt ggttatctgt
    12721 cacaatccat cctgcctgat cttggatatt caattcttta acaaaatcgc taagagggtc
    12781 caaaccaaca tagataaaga caccaccgaa ggcttgttct gtcacttgac ctgttttcac
    12841 attttcaaat acgactgatt ctactcggtt ttcacccttg atttccctta ctacagaatc
    12901 ccagataaag ctgatttttt cattcgcaaa ggcgcgttct tgtaaaacct tttgggcacg
    12961 aagttggtca cgacggtgaa caatggtaac agtcttagca aaacgagtca agaagagggc
    13021 ttcttcaaca gctgaatctc caccaccaac taccaataaa tcttggtcac ggaagaaagc
    13081 accatcacac acagcacagt aagaaacacc acgactgttc agttcttctt ctccaggcac
    13141 tcccaaagga cggtgtttag aaccagttgc tacgataact gtacgtgttt catatgtttg
    13201 gtcatcagtc atcactttct taaaatcacc atggtcttcg acattttcaa cataaccata
    13261 aatgtgctcc acaccaagat tttcaagtgg ttcaaacatc ttttcagcca attcaggtcc
    13321 actaatatta gcgtatcctg ggtaattttc gatatcagat gtattattca tctgaccacc
    13381 tggcagacca ccttcaatca aagctacttt tagattgctt cgagcagcat acaaggccgc
    13441 agtcatccct gcaggtccag caccgataat aatagtatcg tacatataga ttccttcttt
    13501 cttgttgtaa ctatctttat tctaactctt tcttgtcaat cacgcaagga ttatgccttg
    13561 aaaacaagaa ttaaactcat aaccgcaaag gataatactg ggacaaccaa gactccaatc
    13621 ataatcatat catagagatg ggcttggcga gccttggctg tcttatcaac tcccgacatg
    13681 gctctcagac caatccaaat ccctaaaaaa atcagaacaa ggatggtggt caagatcaaa
    13741 ctctcgaaat ataaagaaaa tagttgcagt agcatgattt ctctcatttc tatctttttt
    13801 aaagagtaaa ctcagctagt ccaactaact gagttttcct ttatctatta tatcaaatat
    13861 aagtccgttt gtaactagcg aagaattctt ttgtccgctc ttctttaggg gtgtggataa
    13921 tctcatccgg tgttccagac tcgatgattt tccccttatc taagaagaga atcttatcag
    13981 ccacttgggc tacaaaggac atgtcatgac taaccaaaat catggtttga cctgacttag
    14041 cagcatctgc aatagacttt tctacttcac caaccaattc tgggtcaagg gctgaagttg
    14101 gttcgtctaa gagcaaaaca tctggtttca tagcaagcgc acgcgctagg gcaacccgtt
    14161 gcttctgtcc acctgataaa tggcgaggat aatggttttc acggtccgaa agcccaacct
    14221 tagccaactc ttccttggca atcttagtcg cttcttggtc agataatttc ttgacaacaa
    14281 ccaagccttc tttcacatta tcaagtgctg ttcggcgttc aaacaaatta aactgttgga
    14341 aaaccataga caacttacga cgtagggcaa ggatttcttc ttgagtgatt ttagaaaaat
    14401 caactgaaaa accatcaatc tgaatagagc cactgtcagg tgtttcaaga taattgagac
    14461 tgcgaagaaa ggttgatttt ccagctcctg aagaaccaat caaggctaca acttcccctt
    14521 tttgaatatc caagtccaga tgatccaaga cagtctgtcc tgaaaaggat ttgcttaaat
    14581 ttgaaatctt aatcattaac gaaggtctcc tttcacatct gtttgcactg tatcaggtgc
    14641 agaaatagcc atttttctct cgatgaaacg accgaggctt tcaattccga tattgaccac
    14701 ccaataaaca agggcaacgg agatgaagcg ttcaaaatag cgataatcag ctccacctag
    14761 aatctgagct tgggcaaaga cttccacaac acccgcacta aaagctagag atgttccctt
    14821 ggtcaaaccg atgagggaat taatcaaggt tggagtagct accaccgctg cattaggaat
    14881 aatcactcgt cgataaactt gcgctcgggt catacccaga ctgcgtgccg cctcaatctc
    14941 accaggatta actgagagaa tggctgcacg aatggtttca ctagcataag ctgcctcatt
    15001 aaaggcaaag gcgacaatcg caaaagctgc agctggaatc gcattgatat tgagaccagt
    15061 tccccattgc tgattgaggg ctttcaaagc caaagggatt ccgtagtagg tcaacatgag
    15121 ttgcaccaaa atcggtgtcc cttttaagaa actaacaaag aaggcctgca agggatataa
    15181 aatcttgaca cgattgatct tcacaatggc aaaaagaagc gccaaaacca agccaaaaag
    15241 ggcaccacca attgtcaaca taattgttgt tggaagttgt tggacaattc tagggattcc
    15301 atcaaagacc gaacgtaggc taaacagctt gccatccgga atcaattgca tcaagttttg
    15361 gtaccaatct gatgctaaaa tcgttgtaac attcataaaa acatcctctt ctgataatga
    15421 ttcattctac catgcttctg ccgtcaatga aattatttgc tacggtcaga tacagacttt
    15481 gtctacctac tagtttatca tactttgaaa cagggaagtt atatatttta tctatcaaag
    15541 agatagataa aaactatttc agtcccagtt cttcataaga ttttcgataa ccgatttgct
    15601 taacagttcc attttctact aaaatgggcc gttttaacaa cataccgtca cttgctagca
    15661 actcagccgc ttctcggttt gacaaacttc ctaccttatc ttttagccct aattcacggt
    15721 atttgatccc actggtgttg aaaaattgct tcaattcaaa tcctgaggtt tctagccaat
    15781 tcaaaatgac ttcttggcta ggtgtttctt ccacgatatg gacggcttta tagtccacac
    15841 ctaattgatt taattcttgt tttgcttttt tacaagttga acatttgggg tattcgataa
    15901 attctaacat gtgcttctcc ttctattgtt tctatcttta aaatctactc cttcatgctc
    15961 caagagccaa gctttctttt ccactcctgc agcatagcct gtcagacgct tgcctgctcc
    16021 caacacacga tgacaaggta ctaggataga ccaaggattg cgtcccactg ctccaccaat
    16081 tgcttgagca gaagccactt gcaggtcttg agcaatttgt ccataggtca ctgtctgacc
    16141 ataaggaatt ccctgtaaat aggaccaaac tctcttttca aaattcgttc caattggcgc
    16201 caagagcaag ttggataaat cctgaggctc gcctttaaag taatcatcta agcaagcaat
    16261 aactgggtct aaaataggat gactaacaac ttcttctatc gtttcatctc ctagtcccct
    16321 ctcaaaatgc ttctgctcct gaacccaaat tccatacaaa taatggtcat cagctacaag
    16381 tgatagaata ccaattggtg aggagtagag tatttttaca tacttctttt gcattatttc
    16441 tttaattttt gataggccaa gcgttcgcca tctactggca ccttaccaac ttgtttaaaa
    16501 ccaagttttt caaaaatatg ttgcataacc ttgttttcag catgcgtatc tgagcgaaaa
    16561 tcaagataat caaaaccttc aatcaagccc tctaagaagg tttgagcaac tccttttccc
    16621 tgcacatctg ctgccacagc aatacggtga aagactagat actctgactc tccagcttgc
    16681 cagtttccct cataaatagc ttcataggcc tcctctggac tcttggtcac agcagcatag
    16741 gctagcagtt ctccctcttc caaggctacg taggcttgcc ctgagataat atcatcgata
    16801 ataacatctg catttggata gccattttgc cactggtcac taccagcatc tgctaaacat
    16861 tttttggcat cctccatcac ctgcatgatg gcatctactt catttggaaa agctaaacga
    16921 atttccatct tttttcctca tttctgacta gtttctccca tcatagcata atttaagtat
    16981 ttctacaagc agttaaaact tgactttgtg tttttgttat tttataatct agttatcaaa
    17041 actatataaa aaggagttga aaatgaaaac taccttttcc tacccaaaat gggcagaaat
    17101 tccaaacatt gacctctatc tggaccaggt tttgctctat gtcaatcagg tctgcgcccc
    17161 tatctctcct aataaagaca agggcctaac agcatctatg gtcaacaatt atgtgaaaaa
    17221 tggttacctg acaaagcctg acaaaaaaaa ataccaacgc caacagattg cccgtttgat
    17281 tgctatcaca accctcaagt ctgtattttc tattcaagaa atagcccaga cacttaatac
    17341 tctacaaact caagcaagtt cagaccaact ctacgatgct tttgtggact acatgaacca
    17401 aggaattgac ccagctaacc ctattatcca aaccagctgc caaaccgtta aactctatca
    17461 tcaaactcta gacttaatcg atcatactca agaggaggta atccaatgaa cactagtctt
    17521 aaactcagca aacaactcag ttttggagag gagattgcta atagcgtgac ccatgctgtg
    17581 ggtgcagtca tcatgcttat cttgctgcct atttcatcca tctatagtta tgaagcacac
    17641 ggatttttat cctctatcgg cgtttccatt ttcgtcatca gtctctttct catgttccta
    17701 tcatccacca tttatcactc tatggcctat ggttcgaccc acaaatatgt tttgcgaatc
    17761 attgaccatt ctatgattta cgttgccatt gccggctcat acacgcccgt tgtcttgacc
    17821 ttgatgaata actggtttgg ctatctgatt attgtcatcc aatggggaac gaccatcttt
    17881 ggtattctct ataaaatctt tgctaaaaag gtcaatgaga aatttagcct tgctctttac
    17941 ctgattatgg gctggttggt tctggctatc attcctgcca ttatcaatca aacgacaccc
    18001 gttttctgga gtctcatggt aactggcgga ctctgttata cagttggagc tggattttat
    18061 gccaagaaaa aaccttattt ccacatgatt tggcatctct ttatcctagc tgcgtccgta
    18121 ctccaatata ttgctattat ttattacatg taaaactctc agtaggcaga gcctgcttag
    18181 agttttacaa acgaatttcc caaagaggaa gcaatatgaa agcagtcatt gcttgtgttc
    18241 ataaaattct cagaatcatt tacaaactac tctccaccaa gtagacttat caaaaagaaa
    18301 aggcgctagg actgaagaaa cagttctaac gcccaaatta aaaaaatttc aactacagta
    18361 tagcacagga aatgattttt tgcgcttttt tactgttttt tcaaataaaa aaagacaaag
    18421 ttttatcctt gtcttttagt tttagtattg ctttggtttc aatgtcaata agtgtgttct
    18481 ttttggttca gtaactattc caatttatct atcttgaaat aaataaaatc cctttgtaat
    18541 actataaaaa ataactaaca aagaaataac tataagactc ggttgccaga aacctgatat
    18601 atctactaaa ctacgaccta aaaattggcc aataatgcta actaaaatta aaataattaa
    18661 attgaaaatt actgacacta ataaaattaa caccgaatgt attattgcct cgcctatacg
    18721 tattttaaaa agttgtaatc ttgacactcc cagtgtcttt aactgtttaa tgtcctcaat
    18781 atctttatta gaagataaaa ttgtaagaga aatagaacta gtaataatca gaataattgg
    18841 tgcagacata taagcaataa aggaagctct tgcttcgttt actccgtttg ctgtattcat
    18901 gaatgaatac atattttggg tgtaaagaat aaacccagaa atgagagtta ctcccatgct
    18961 cattgaagtt tgtatacttt tcaaatagct aggattggat agtagattcc aaaagcctgt
    19021 attaattaca taattttcag ttggaaatat cctcattaaa aattttatta gaaacatttg
    19081 aattgatgga gatagtgatt gaataataag gatgtgaata attaataaaa ataatattat
    19141 gcttgactgt ttttcaatta tttctttgtt tctgataaaa cctgctgaaa caatagaacc
    19201 tatacatagt agccatagga ataaacgaac agagatgtta acaaatccag ttactgtaac
    19261 ttttcttttt ttcttttttg gttttaatat tgatgatatt ttaagtattc gacttgaata
    19321 aaagtaagcc cctatcccga caattgttgg gactataaaa atactcaata aaattgattg
    19381 aatgttggct gtaataacta aatcaggtaa ctcatctctt cctaataaat tttgtaaaaa
    19441 tttgtaataa ctatctgcca tgataaatga aagtattgta ccaatactac taacaatcac
    19501 tgccattaaa taaaactgtc cagatactaa taaagatagc tgagttctgc ttgcgcctaa
    19561 gatagtccat aattggtaat cctttttaaa gatatctatt agcagtctta tattatttga
    19621 aataaggaaa aatagagtag tcccgccaaa aattattagc atttgaaaaa gttgtgaagc
    19681 gttaatatta gcagtcttga ttgaactagc aataccaaat aaagatgttc caacaatcag
    19741 gctagataca aatagtaatg gtattgttcc taaccattgt ctttttgagt actgaaattg
    19801 gtaaatgatt aattttagca taataaatcc tcctcaaatt aatctttact actttcaagt
    19861 gcttggtaga gctcatcagc tgaaggttta ataatttgtc gagatatttt tccgtctttt
    19921 aaaatgagtg ctctgtcagt ttttgaagcc aactcaatat catgagtgac cataagaaca
    19981 cattttcctt gactcgctaa attacgaagt gtttcaaata taagttttct tgagacgctg
    20041 tccagagctc ctgttggctc atcagcaaaa ataattttac tatcagctat tattgcacga
    20101 gtaatagcca ctttttgctg ttctccacct gataaggtag taactaatga tgatagttct
    20161 gctttaaaat tcaaactatc caacattttt tttactttat tgctatcaac actctttcct
    20221 gatagtctca gaggaagtgt aacattttct agtacaggta gagcaggtac cagattatag
    20281 ttttgaaaaa ttattgcaat atcttgtctt ctaaatttag caagcttccc ttcttttaag
    20341 gtatatggat ttacaccatt gataacaact tcgccacttg ttggttcaga taaacttgat
    20401 aagcaactta gcaaagtaga ctttccagaa ccactgacac caagtattga tacaaattcc
    20461 ccataattga cagataagga aatatctttc aaaatagaga cagttttgtc ttgccctaac
    20521 aaaaattctt ttgaaaggtt tgagacttta acagctacat tattaaccat aataaatcct
    20581 cctgattcca gtgtcatctt tgatttaatt atacaatatt ggaattctat taatatcaag
    20641 taagtaactt atgaaacgta tttttaatgg ataaaatttc tatttcctct ggatctcgaa
    20701 taaaggtctt tagcgaacta agaatttttt ctattaccat aatagtatca taaatgagac
    20761 ttgattgaaa gcgtaaacat agctttgtta agtgatacaa ctattctata caaaatttaa
    20821 acataaaact taggtatact taataaatga gaatttaagt tagctataac aaagatagta
    20881 gcatatgtaa aactctaagc aggctctgcc tgcttagagt tttacaatcg aatttcccaa
    20941 agaggaagca agatgaaagc cgtcattgct tgtgctcata agattctcag aatcgtttac
    21001 aaactactct ccaccaagca gacttatcaa aaagaaaagg cgctaggact gaggaaacag
    21061 ttctaacgca aaaactaaaa aaatttcaat tacagtatag cacaggaagt gattttttga
    21121 gcttttttac tgtctttttt ctttacacat ttcgataaag tactggtgca agcgcacatc
    21181 atcagtcaat tctggatgaa aagaacttac caacatattt ttttcttggg ctgcaacaat
    21241 ttgattgttc actgttgcta aaatttctac accctcacca acactactga taatcggacc
    21301 acggataaag gtcattggaa tcttgccaac tcccttacat tctgcttccg tgtagaaact
    21361 tcctaattgg cgcccataag cattacgctc gaccaccata tccatagttc ctagatgact
    21421 ctctttctga gaagtgattt ccttagccag caaaattaag cccgcacagg tcccaaacac
    21481 tggtaagcca gatagaatgg cttctcggat gggaagtagc atgttctggt cacgtaagag
    21541 cttgcccatg gttgtagact caccaccagg caaaatcaaa cccgacaagt cactctgatc
    21601 ttgctgaaaa tcatctagat ttctgagttc tacactctcg acacctaatt gatctagcac
    21661 ttttgcatgt tctgcaaagg ccccttgcaa ggccaatatt ccgattttca tctattttcc
    21721 tcgttcagcc atgagaattt ggatttcatt ttcattaata ccaaccatgg cttctcctaa
    21781 atcttcagag atttgagcta ggatttgagg attacggaag ttagtcacag ccttaacaat
    21841 ggcactcgct cgtttaacag gatctcctga cttgaaaata cctgaaccga caaagacccc
    21901 ctctgcccct aattgcatca ttaacgcagc atctgctggc gttgcaacac ctccagcagc
    21961 gaaatttaca actggcaatt ttccatgttc atgaacatat tggaccaatt ctacagggac
    22021 ttgcaaatcc ttggcagcaa cataaagctc gtcctcacgt aagttttgaa tgcggcgaat
    22081 ttcctgattc atcatacgca tatgacgaac agcttggacg atatcccctg tccctggttc
    22141 tcctttggta cgaatcatgg aagcaccttc agcgatacga cgcaaggctt cacccaaatc
    22201 cttagcacca cagacaaaag gaacttggaa ttctttcttg tccacatgga aacggtcatc
    22261 agctggagat agaacttcac tctcgtcgat ataatcaatt tcaatagcct ctaaaatctg
    22321 agcttcaaca aaatgcccga ttctgacctt agccattact ggaatactaa ccgcttcttg
    22381 gatttcctta atcatctttg ggtcgctcat gcgggaaact cctccagctg cacgaatatc
    22441 agccggaatt cgttccaagg ccatcacagc tgccgcacca gcagcttctg cgatacgagc
    22501 ctgttcagga ttctgcacat ccataataac accacccttg agcatctgtg ccaagttttt
    22561 atttagttca taacgatttt cagtcatttg ttttatcctc atcatttgta ttggtagcta
    22621 ccttttcatt ttaagtcaga acagaatgaa gcacaagata aaaaaactat aattgtatgg
    22681 ggacaaatta gctaaaaact atctgacctt aacttaaggc cagatagctc attcattttt
    22741 atttttcagc cgtaagggca gccattgtga tgtagttgta tggtttgttg aagtgtggca
    22801 agaagaagag gtctgtcaat gccaatttat caattgtcac atgctcttgg atagcaagtg
    22861 agaacatgtg gattcccatg ctaattgcaa tatcatgtga aaccatttgg gcaccaagaa
    22921 tttcacggct atctttgtca aagacaatct taattgctac ttcatggttg tcatgtttca
    22981 tgaattctgg tttttgaaga tcgttaaagc ctgtttcagt tgcgttgtaa ccagcagctt
    23041 tcgctttttc aagagtcaaa ccagttgaaa ccatgtgaag accgtagatt gagataccat
    23101 ttgaaccttg aacaccgatt ccttccaatt catgtccaca agcattgtag gcaccaacga
    23161 taccagtgcg aacagcattt gaagcaagag cgatatagct tgtatcttta cgagcattgt
    23221 cataaacagt cgcacagtca ccaacagcgt aaacgcctgg gatagatgtt tcttgtttct
    23281 tgtctacaag gaaggcaccg ttgcggaaga gttcgatctt accatctgca agggctgtgt
    23341 ttggacggaa accaactgca aggataacca tatccacgtc aaagctttct ttgtcagtaa
    23401 tcaagcgttc aactttaccg tcaccttcga ttgctttaac agtttgacct agagccaagc
    23461 ggatgttgtg atcttccaag ttcttcgcca tcatttgtgt gaagtctttg tcatagtaac
    23521 cgttcaagac agtatcaacg atatcaacaa ggacaacttc ttttccaaga cgttcaaagg
    23581 cttcagcaag ttcaacaccg atgtaaccac caccaacaac ggcgatacgg tcgaggtgtt
    23641 ggctcttgtc agaaagttta ttgataactt cttcagcatt ttggtacaat ttcacgaatt
    23701 gtacgttttc aagagttgct ttaaattcgc ggtttccttt aacaatttca acaccttcga
    23761 ttggtggcaa gattggtgta gaacctgtag cgaaaatcaa tttttcgtat gattctttgt
    23821 gctcttttcc ttcaacttcc gctgtaacta ctttgttatc atagtcgatt gaaagaacag
    23881 gtgagttcat gtaaacttta gcacctttag cttccaattt ttctttatca gaatagaaca
    23941 agccttcagc accgtcaatt tgttcaccaa tccaaagagc cattccacat cctaggaaag
    24001 agatgttaga gttttggtca aatacaacaa tttcgttctc atttccaaaa ttatccaaca
    24061 tggtattgat acatgctgta ccagcgtggt tagcaccgac tacaacgatt ttactcataa
    24121 aaatttcctg ccttcattct aaatttacct tactagtata ccatttaccg ttagcgttta
    24181 caaggggggt actattattt cagcattttt tatcaaaaat aattccccat ttatcctgtt
    24241 ttcaagcctc atgaatttta tgagcatatt tcttgacttt ttccaaattt tgtttgtgaa
    24301 aattctaact ttatggtatt ataataacat gaaaatgaaa tgtaagggct tcaatttaga
    24361 taattatttt atattacaac ttattttcta taagagaaag gagttggtag cttgactctt
    24421 cgttcacatt ctgaacggct aatggggact actatcacta tttcattagt ggatgagcaa
    24481 gccgatatct ttctccaaaa atcctttgac ttgctcaaag agcttgaata ccgcttcaac
    24541 gccaatagtc aagaatctga gttgatggaa atcaattatc aagcaggaat atcccccgtc
    24601 acggttcatc cagacctgtt tgagctgatt tcacttgggt tagagcatag cctagcgctc
    24661 tctagccacc tcaatatcag cattggtcct ttaattcaaa cctggcgtat cggttttcca
    24721 gatgccaagg tcgcccagcc tcaagaaatt gaatcggtgc tgcctctaat caatcctcat
    24781 ggtatcgagt tagattcttc tacttctact gtgtttttaa aacagaaagg aatgaagatc
    24841 gatctaggtt gtttagccaa aggatacagt gcggataagg ttgcccaatt tcttaggaaa
    24901 gagggggtga cttctgcctt gatcaatctg ggagggaata tcctgaccat tggaaaaaat
    24961 caggcaagag gggataaccc atggcaaatc gggattcaag acccagccaa tcctagggga
    25021 aatcatttaa tgaccatccc tgttgtcaat aaatctgtcg tgacttcagg catttatgaa
    25081 cgtcacctga ccgtcaatgg acaagattac catcacattt ttgacagtca aacaggatat
    25141 cctgttgaaa cggaactagc gagtctaaca atcatctctg ataaatcagt cgatggcgaa
    25201 atctggacga ctcgactctt tggagaaaga cctgcttcta tcctctggca agtcgaaagt
    25261 ttggagggca tcgaagtcat cctcatcgat aaagaaggcc acctaagctg ttcttcagga
    25321 attcctactc tatagagaca actgtttcaa tggaatttta aaattgtatt atgtaaaaag
    25381 agaaaggaaa tctcatgtta aaacttattg ctattgttgg aacaaattca aaacgttcta
    25441 caaaccgtca attgcttcaa tacatgcaaa aacactttgc tgacaaagct gaaattgagc
    25501 ttgttgaaat caaggccatt cctgtcttca acaaaccagc tgacaagcaa gtacctgctg
    25561 aaatattgga aattgctgct aaaatcgaag aggcagatgg cgttattatc ggtactcctg
    25621 agtatgatca ctctattcca gctgttttga tgagcgctct tgcttggttg tcttatggta
    25681 tttacccact tttgaacaaa ccaatcatga ttacaggtgc ttcttacggt acgcttggtt
    25741 catctcgtgc ccaattgcaa cttcgtcaaa tcttgaatgc tcctgaaatc aaggcaaatg
    25801 ttcttccaga tgaattcttg ctctcacact ctcttcaagc atttaaccca agtggcgact
    25861 tggttgacct tgatgttatc aagaaattga atgccatctt tgatgacttc cgtatctttg
    25921 taaaaatcac agaaaaatta cgtaatgcac aagaattact tcgcaaagat gctgaagact
    25981 ttgactggga aaatttgtaa gataggagac caaaaagaat gaaatttgtt ggagttgttg
    26041 gatcaaacta cgatcaatca tataaccgta agctcttgga atttatccgt cgctacttca
    26101 aactcaaatt tgaattagaa gttcttgaaa ttgacgaagt tccaatgttt aaccaagatg
    26161 aaaaatggga cgaaagcttc caattgcgtt tcttgtataa caagattaca cgtgctgatg
    26221 gtgtcattat cgccactcct gagcacaacc acactatctc agcttcactc aaatctgtac
    26281 ttgaatggct ttcatacgaa gttcatccat ttgaaaacaa gcctgttatg attgtgggtg
    26341 catcatacta tgaccaagga acatcacgtg ctcaagttca ccttcgtaaa atccttgatg
    26401 ctccaggtgt taatgcctac acgcttccag gtaacgaatt ccttcttggt aaagctaagg
    26461 aagcttttga taacaacgga aatatcacca acgaaggcac tgttaaattc cttgaaactt
    26521 gcttagataa ctttgttaaa tacgtaggag tcgtttcgaa attgaaaaaa ccaaaaccaa
    26581 tcgaatcaga agacttggat tgtggaaaac caatcgctac aaccatcaca gaagttgacc
    26641 ctgacgatcc agaatgggta gaaaaagttg cagcaatcac tggtgctgtt tctggtgata
    26701 cctatgtcaa attggaccac ggtatcctaa cagttaacca aattgatatg ttcttgaaag
    26761 ctatgccatt tgaattgaca tacgctgacg acaacaacca attcctctac tacaacaacg
    26821 ctcaccaaga tccagacact atgttcgcta aacgtgtacc acctcaatca ggtagccgta
    26881 tgtcgactgt tcatggttct cttccaccag cacgtatgaa gaacgtagag tgggttatcg
    26941 gaacacttcg caacggaaac caagaatacg tccgtacgat cgttccaggt tctcctgcag
    27001 gtgttatcaa cactcacaac taccaagcaa tgtactatcc tgatggatca tacgctggta
    27061 tcaatgaaat cgtctttaac ttccaaccat ggcttgactg gtacctaaaa gagactggtc
    27121 aacgtttggt gggtggtagc ggaccatttg ctcctgctgc cggaggtcat ggagacgccg
    27181 atgctacttc tggcgcttct gattcagggg atgctggagg ccacggtggt gacgcagacg
    27241 ctacagccgg tgcaagttac taatgatagt ttttaggaac cattatggtt ccttttttag
    27301 cgaaaaaaga ctaacaaaca caaactgcta gtctctttgt tatcgtttca tctatccttg
    27361 ttaaaagaaa gaatacttat actcaatgaa aatcaaagag caaactagga agctagccgc
    27421 aggctgtact taagtacggc aaggtgaagc tgacgtggtt tgaatttgat tttcgaagag
    27481 tattattatg aatttgggtc atcaagacta cggccatgta atcctttttc acgttgtact
    27541 tggcgtagtt tttctggtgt aacatcgttt cctttttcgt ccacaatttt gattccttca
    27601 atgtgatgac gaacagcgcg acgataacct tcgatgtact cctcacgtag tttggcttgt
    27661 tccacttttt cttctggtgt taagccttct gtttttttct ttttagcaag ctcattgata
    27721 cgagcaattt ttttcggatc catttcttct cctctgtaat aagataaagt ccgtttatca
    27781 aattttattt agtgttaatt tggttaaaac aagcaaactt agctgctttc ttggttagtt
    27841 gtgacaagat tgccaaacga ttttgacgga cagcctgatc ttcagccatt accatagtat
    27901 tttcaaagaa agcatcaatg actgggctaa gcgcaaaaag ttgtttcaat tgctgacttg
    27961 caggtcctga taaaatgagt gtttctactg cttctgccaa agctttttct tggtcattct
    28021 caaatagtgc tgaatcaacc gtagcaaccc cttctgcctt ctcagccagg ttaaaggcac
    28081 gagaaagtga ttcaacagat ggtttaaaat cttcttcctt gcttacttct acgagagcac
    28141 ttgctgcttc caacatatct gccacaacaa agtttgaacc tgcaagaact gcttccttga
    28201 tatcttttgg agtagagccc atcatcttat caacacgagc cttgataaag tccataacct
    28261 ctgctttatt ttcataagtc aaactgtcaa atttcaatgc ataaaggcta tcaatcagct
    28321 catccatagc aatgtgccaa ccaaaggcat ccaagatacg aaccacacct tgagttgcac
    28381 gacgaagggc ataagggtca ttagaacctg atggaatcaa tcctactgag aagaaactca
    28441 aaatcgtatc caatttgtct gcaatggcta gaactgcgcc gaccttgctc tctggaagtt
    28501 ctccttcagc tgatgtaggc atgtagtgtt cacgaatagc agctgccacc gctggagttt
    28561 caccagcaag aagggtgtat ttttcaccca taattccttg gagttcgtca aattcaccaa
    28621 ccatacctgt caataagtca aacttgtaaa tggctgctgc acgagcaagg tcaactgttt
    28681 catccactga caaaccagct ttttctgcca aaagtacagt gatttgaccc gtacgaatca
    28741 tgtgttcacg aagggaacca atcttctcat ggaaggtgac attgtttaat ttttcaacaa
    28801 gatctgaaat caccaatttt tggtcttcac gccagaagaa ttctccgtct tccaagcggg
    28861 ctaccaagac tttttcattt cctttaatga catttttcaa acgctctgcg tttccgttac
    28921 gaacagaaat gaagtttggc aagagttttc catcttgatc acgaacaaca aagtaacgct
    28981 ggtgttcctt catagaagtc accaagattt cttctggaac ttcaaggtat ttagcatcaa
    29041 aacttcccat gaaggcagtt gggtattcaa ccaaattcaa gacttcattc agcaaatccg
    29101 catcaatttc gatacgtaca ccatgttttg cctcaatttc cttgatttgg tcaacaatca
    29161 tttgctcacg ttcacatgga tctgcgatta caaactgctt acgaaggtct tcttcatagc
    29221 tcaatgctga ctgaattttg gtttctttcc ccaagaaacg atggccacgg ctcacacgac
    29281 ttcccttgat atcaaggaaa tccaagtcaa actcttgctc atccaagaga acagttaaag
    29341 tgtggacagg acgaatgtat tcaaagctat ttccagccca gtgcatgctg acagggaaag
    29401 tcaatgactt caagacatct acaacaccag gaacaatggc ttcaactgct tgaccaactt
    29461 cttccttagt gacatagaca tattcttcac ccttgatttc acggaattcg atatcttcaa
    29521 cagtcaagcc ttttccacgg acaaatcctt gagctgcttt ggtgaagttt ccatcactat
    29581 ccaaggcaat tttctttgct ggtcccttga aatcttctgt caaatcagac tgtttgtctg
    29641 ataaaccagt cacacgaaca gccaaacgac gtggtgttga gaaggtttga atggcttcaa
    29701 aagacaggcg gttttccttg aggaaggctg ccattttttc gcctagttgt ttttcacttg
    29761 gtgtgacaac ataggctggt aattcttcaa gaccgagttc tactaataag ttttttgtca
    29821 tgttttttct ccgaaaagta tctttttatt ttccagtttg ccttatgtga ttggcacgca
    29881 agcaaccaac ttcgttgttt cattgctaaa attggagcca aaagtctcca attttgccta
    29941 gtatccttag tttcactaag gataccttca tcactgtggc aactggctca gggctcactc
    30001 tgttcgccca ttttcgtaat ttgtcactct ctttattctg cgtcttctgc taaaagttta
    30061 actcgtgttt cttcatccaa aagtgggtaa cctaggcgtt tgcgttctgc aacaaaggtt
    30121 ttggctacaa cacgggccaa gttacggata cgagcgatat agcctgcacg ctctgttaca
    30181 gatacggcac cacgcgcgtc aagcagatta aaggtatgtg aacatttgag aacatagtca
    30241 taggcagggt gtaccaagcc ttcttctaat gcacgaccag cttctttttc aaacttatca
    30301 aagttttcaa gcaacatttc ttggttcgaa atttcaaatg aatattttga gtgctcatac
    30361 tcaggctgga taaagatttc tccgtatttt acaccatcag cccactcgat atcatagaca
    30421 gagtctactt cttgaatgta agaagccaag cgctccaaac cataggtaac ttccgcagtc
    30481 acagggccag ttgccaatcc accgacttgt tggaaataag tgaactgagt gatttccatt
    30541 ccgtcaagcc aaacttccca accaagacca gctgaaccag ttgatgggtt ttcccagttg
    30601 tcctcaacaa aacgaatatc gtgctccaaa ggattgattc ccaatttttc caaagactca
    30661 aggtaaagtt cttggatatt tgatggagaa ggcttcatga ccacctggaa ttggtggtgt
    30721 tggtagagac ggttagggtt ttccccataa cgaccgtcag caggacgacg tgatggctct
    30781 acataagctg cattccatgg ctcaggtccg atagcacgaa ggaaagtgta aggactcatt
    30841 gttcccgcac ctttttcatt atcataagcc tgcataagca tacaaccttg gtcattccaa
    30901 aattgttgca aagtcaaaat aatttcttga aatgttaatt tcttagacat tgaatactcc
    30961 ttaataaaaa ataatttctt gcgacacgag tctgcctcgt cgattatacg aggcaagacg
    31021 agttagtact gcgtctagct caggcactct agtgctgagc gtggagcagc ccgactgtaa
    31081 ggaggtctct gccactgatg cagtgaaatg ttaatttctt agacattgtt tactccttta
    31141 gtttttatat ttctttttca attaatttct gctgaacaaa ccaatctaac aagggaaaag
    31201 ttttttctgt atttgcccat agatgataag aatcaaagac cgcttgtaca ctacctaaat
    31261 tttctaccaa cttatctact atcaaaaaac tgagccaata ttcataaaaa atacttgcat
    31321 agttcttatc ataagtcgaa gaaccaaaat cattcaatga atgccaacca tacttcttct
    31381 gaaaaagttc tactagagat tgattacaaa ttttttccgc ttgaaattcc tcttctgtca
    31441 agaaatactt gcgactaata tattcaacca tcccctcttc gaaccagata taagagtcat
    31501 aaccatcaaa atcatctaaa aaatgctccg accaatgagc taactcatgt cctacaatct
    31561 gtaagaggaa attttcagat aaagaatgat agtgactttc tattgcttga gtttgctcag
    31621 aacactcgta gtccatcaat tgatacaaat acaactcttt ccaaacagct aaatcaggcg
    31681 tcataaccat tcgtctatta tttgtatagg ctggaacagc gctttcccta ataatctgtg
    31741 tagcagcatt aaaatttgac caaataacgg cttgcggtaa atcacagact gcaagttcat
    31801 tctttaaaaa agattgataa tctttcaact tttcagtatt tcttactaca aaatcacgaa
    31861 atgcagctag ttgactatcg tcttttacaa gataaaggtt ttccacgtct ctcctttctt
    31921 tcacacaata gactgaataa gcaaaagaac cacatctgac aatcctaggt tgatttaacc
    31981 taggggcgtc agaaacgcgg ttccacccta atttattact tcattgtttt tgaaaatagt
    32041 acagaaagcg ccagttcttt attttgctct acttggctcc cactatccca agctcgctta
    32101 aagaacgcca taagactttc tttcctgctg actattatat caaaaattag aataatgtac
    32161 aattcttttt atgattttct aaaaatccat atcatcaact cgtggagcac cagaatgaac
    32221 agctatcgtt tttaaggttt catgttcctc atggctcaat tcaattccaa agcaatcaag
    32281 attagcctga atacgagata ctgtaacaga tttcggaagt ggtaaaaatc cttctgccaa
    32341 gctccaggcc aaggctatct gagcaacaga ctttccatga tttgctgcga tttcttggac
    32401 ttgcttgcta tcaaacagtt ctccctgacc aaaaggtccc caagcttcca ataaaattcc
    32461 tttttctcta cagtaagcta caacttcctc ttgatagaca cctggcgcca agcggatttg
    32521 attgaccgcc ggaagaattt ttgctgtttc aagcaaggca tccaaatgat ggggaagaaa
    32581 attactaaca ccgatagcac ggattttccc ttcttgatag aggtcttcca tcgctctcca
    32641 aacttctgca tttcggattt tccattggtc attttctctg agcggttttg gatttggcca
    32701 atgaatcaaa tacagatcca aataatccaa gcccagtttc tctaaagact cttcaaaagc
    32761 ctgacgagct tgctcatagg tttgttggct attccacagc ttggtagtta cgaacaattc
    32821 ctctcgtgga actccgctat ctttgattgc ttgaccaaca ctttcttcat tctgataaat
    32881 cgccgccgta tcaatatgac gataaccagc cttcaaggct tctaacactg cacgataggc
    32941 ttcttctcca tccttagcct taaaagttcc aaatcccaat actggaattt ctactccatt
    33001 atttaattga taggtatcca tatgcttctc cttctatttt atactctctt caaaccacgt
    33061 caacgtcgcc ttgccgtagg tattgttact gacttcgtca gttctatcca caacctcaaa
    33121 acactgtttt gagcaacctg cagctagttt cctagtttgc tctttgattt tcattgagta
    33181 ttattcatca attatagcat aaaacatttt ttaagtagta ctatcttttg taaaaaataa
    33241 gtctatcaca tatctgacag acctattttt tcttgcttat tcatcacgac tataatacag
    33301 ctcatgagct tttaggcgag tattgagcat ctctggtatg gtcacaaagg tataaccttg
    33361 atttttcaaa tactcaatga cccttggcaa ggcattgact gtcggactgt gaatatcatg
    33421 catcaaaacg atagagccgt tagctacttg atgctgaatt tccgtcaaaa tagctgcttc
    33481 atttttactc ttccagtcca gactatccac atcccacata ataaagctca aatcgaggct
    33541 attgcgaata tcatctgtaa tagctccata aggcggacgc atgagtttgg aactagaacc
    33601 cagcacttta gttagcacat cctcagtatc agtaatctgc tttttagctt catcaagaga
    33661 tagttgcgag agaatcggat ggctccaact atggtttcca acaacatgac cttcagattt
    33721 tatcctcttc accaagtcct cattcccaga aacatttttc ccaagcacaa agaaagtcgc
    33781 tttaatatca tatttagcta gggtctctaa tacctgcggg gtcgttgctg gatttggacc
    33841 atcatcaaag gttagagcga caactttttg atgtttctta tcaaagtaag attggtacaa
    33901 ggccgcatct ttttcgagta agtacgaaga ttggataaca tcaaagaaag cagatactgg
    33961 caaggctatc tcttctaaat tttcaaccac aggacttgga taaaggataa tctgactatc
    34021 cttgtaatca aaattccatg cagacaagtc ttggtcagag aagtttttta caatctgctc
    34081 actctggtct tgctctattt ttttatcctc tatgaaggag gtcaactctt ttatcagctg
    34141 ttccttagcc ttactagcat ctgaaaacag ttggtcaagt gtaaaaggtt gcccatcttc
    34201 tgttaagtga acctttccta gactggtctt ttcagtctct tcaatcttaa aagcaactaa
    34261 atcatagatt tgtttggtca cattacgatt aacgactccc tttaaacctg actcttcttg
    34321 ctctgtatag tagaaaacaa gattgtcctt actttctagc ttgtccttaa catcttgatt
    34381 tatcagctcc ctaacagagg aaatcacttt ctccccttgg agaggataat aggcaatcac
    34441 ttcggcttgc ccctgacgaa aatgctcctt ctgatttccc tcactcaatt gatcatcttt
    34501 ctcttttttg agcgattcaa tcttttgctc aaaacttttt tgctggtaga tcttaaaagc
    34561 aataaatccg cctaataggc aaatagaaat tgctaaaata ccaatcaaag ccaataatac
    34621 atgtctcgtt ttcccgtgtc tgccacgtcc tagtctactt ttattcatat ttatatcata
    34681 acaaatttga gcaataattt caatgataca atttggcaat ccacaattcc tctagcaagc
    34741 caaagcatct tacatcaact ccaacatccg tatctgtaga gattatgtag ttttctatct
    34801 atggaatttc tagtagattg aagctagaat agtagaccta tacttctaaa acattgttag
    34861 aaatcgattt gactgtcctg atcgatttgt cctgttctca tttcatttta ctataaaact
    34921 atgaaaagct aggtcatctg agaagtaatt actatcgaca attgcgatag cgtatactag
    34981 tgcgatttgt ttgtagctct ggtgggtatc accttcacgt tggcgaattt tttctaggtt
    35041 ttgattaacc taactacaca gataagccca taggcgattg atgaaaaaat tctgatgatg
    35101 aacctgaatt tcgatgataa cttggattcc attatctaac tccgccaaga cgtctatact
    35161 agtatagaaa tcctgcgctg agtagggcat ggaaggcaag acgtgaatgt tacttccctc
    35221 caaaatcgtc acattttttg ctggcaagtc cagcatatcg cggataaatt gacaaatcat
    35281 tagttgggct gatacccgga tgtctgagaa tcctcctttt cctccttcta ttatagcaca
    35341 tttttaaatc taggtttgct agattctatg cttctatcta tttattcgga aaagaaagct
    35401 tttcttcaga agaaagctaa tagcttattt gaaattttct cctctctatt ggatagtccc
    35461 gtctatctat gttataatga aagaaggatt taaaatcgga aaaggagtat ttatgctaaa
    35521 attaggtgtc atcggaactg gcgctatcag ccatcatttc atagaagcag cccataccag
    35581 tggagaatac caactggtcg caatctattc tagaaaacta gaaactgcag caacctttgc
    35641 ttctcgctat cagaatatcc aactattcga tcaattagaa gtcttcttca aatcttcttt
    35701 tgatttagtc tatatcgcca gtccaaactc cttacatttt gctcaggcaa aagctgcctt
    35761 gtctgctggt aaacatgtta ttctcgaaaa gccagctgtc actcaaccac aagaatggtt
    35821 tgatttgatt caaacagctg aaaaaaataa ctgttttatc tttgaagcag ctcgtaatta
    35881 ccacgagaaa gcctttacta ctatcaaaaa ctttttagca gataagcaag tgttaggagc
    35941 agatttcaac tatgccaaat actcttccaa gatgcctgac ttgttgactg gacaaacacc
    36001 aaatgtcttt tcagaccgtt ttgctggtgg agccctcatg gatttgggga tttatcccct
    36061 ctatgctgct gttcgtcttt ttggaaaagc caatgacgcg acttaccatg ctcaacagct
    36121 tgacaatagc attgacctaa atggagatgg tatcctcttc tacccagact atcaagttca
    36181 catcaaggct ggaaaaaaca tcacttccaa tcttccttgc gagatttata caacagatgg
    36241 aaccttgact ctcaacacga ttgagcatat tcgctcagct atttttaccg accaccaagg
    36301 aaatcaagtc cagcttccta tccaacaggc tcctcatacg atgactgagg aagtcgctgc
    36361 atttgcacac atgatccagc aaccagatct gaatctctac caaacttggc tgtatgatac
    36421 aggctctgtt catgagctac tatataccat gcgccagact gctggcatta gatttgaggc
    36481 agaaaaatga aaaccaaact accgactgaa tggcaagaac tgagtgacca actcggtttc
    36541 caagaattca cccccattca aactcaacta tttgagcccc ttcttgctgg agaaaacctc
    36601 ctaggagtga gccaaaccgg aactggtaag accctagcgt atctcctacc aagtcttctc
    36661 agactacaaa agaaaaaagc ccaacaactc ttgattctag caccaaatac ggaacttgct
    36721 ggacagattt ttgatgtatg taaaacgtgg gcagaagcta tcggattgac tgctcaactc
    36781 ttcctttcag gttcaagtca gaaacgacaa attgaacgtc ttaaaaaggg gccagaaatt
    36841 ctgattggaa ctcctggccg tatctttgaa ctcattaaac ttaaaaaaat caagatgatg
    36901 aatgtggaaa ccatcatcct ggatgaattt gaccaattac tagatgattc tcagattcac
    36961 tttgtagaaa aaatcactca ctacgcacct cgtgaccacc aactggtcta catgagtgcc
    37021 acgaccaagt ttgaccaaga aaagattgtg ccgaatacac gcactattga tctctctgat
    37081 caaaaattgg acaatatcca gcatttctac atgcaggtag accaacgtca tcgagtggat
    37141 atgctgcgaa aactggctca tgtagaggat ttccgtggtc tagtcttctt caatagcctg
    37201 tcagaccttg ggaatgccga ggaaaaacta cagtatcgtg atatattggc tgtttccctc
    37261 gctagtgatg ttaatgttaa atttagaaaa atcatcttag aaaagtttaa agataaccaa
    37321 ctaaccctgc ttcttgcaac tgaccttttg gctcgtggaa ttgatatcga tagcctagaa
    37381 tgtgtcgtaa actttgatat tcctagagat agcgaaacct acacacaccg tgctggccgt
    37441 actggccgta tgggcaagga aggatatgtt attactctcg tcactcatcc agaagaaatc
    37501 aaaaaactta aaaagtttgc aagtatccga gaaattgtcc taaaaaacca agaactctat
    37561 atcaaataaa gccccattgc tctatacaca tccagtgtat ggagcttttt tataagcaat
    37621 tcaattttat actcatctgc tttcaaaaag cattctattc catctccgat taacgatgga
    37681 ctttatcacc tccttctcca gtccttgtat gacatcttgg agttgattca tgacatcttc
    37741 caaagttcga aaggctttat tcttaaatcc acgtttacga atctctttcc acacttgttc
    37801 aa
//
LOCUS       DAVDWT010000024        35918 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVDWT010000024 DAVDWT010000000
VERSION     DAVDWT010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35918
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.024"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            1..864
                     /locus_tag="VUW50_001356"
                     /pseudo
     CDS             1..864
                     /locus_tag="VUW50_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            960..1298
                     /locus_tag="VUW50_001357"
     CDS             960..1298
                     /locus_tag="VUW50_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET7983242.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQETLDKHYQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            1488..2924
                     /locus_tag="VUW50_001358"
     CDS             1488..2924
                     /locus_tag="VUW50_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET7983243.1"
                     /translation="MTTFKDRFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITSGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSLAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTAQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            2976..3215
                     /locus_tag="VUW50_001359"
     CDS             2976..3215
                     /locus_tag="VUW50_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HET7983244.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            3345..3659
                     /locus_tag="VUW50_001360"
     CDS             3345..3659
                     /locus_tag="VUW50_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HET7983245.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            3777..5756
                     /locus_tag="VUW50_001361"
     CDS             3777..5756
                     /locus_tag="VUW50_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET7983246.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFDQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNGFRQYFSKENFLICTESDKVNLLEKMVEGLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            5766..6080
                     /locus_tag="VUW50_001362"
     CDS             5766..6080
                     /locus_tag="VUW50_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HET7983247.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            6111..6617
                     /locus_tag="VUW50_001363"
     CDS             6111..6617
                     /locus_tag="VUW50_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983248.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIAPFFFGNIYWL
                     LNQFISPSPIIIFPIMLIVFSILATVEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            6697..8043
                     /gene="celB"
                     /locus_tag="VUW50_001364"
     CDS             6697..8043
                     /gene="celB"
                     /locus_tag="VUW50_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HET7983249.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            8422..8598
                     /locus_tag="VUW50_001365"
     CDS             8422..8598
                     /locus_tag="VUW50_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983250.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALSLGMT"
     gene            9000..11213
                     /locus_tag="VUW50_001366"
     CDS             9000..11213
                     /locus_tag="VUW50_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HET7983251.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(11435..11911)
                     /locus_tag="VUW50_001367"
     CDS             complement(11435..11911)
                     /locus_tag="VUW50_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET7983252.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRVEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            12134..15337
                     /locus_tag="VUW50_001368"
     CDS             12134..15337
                     /locus_tag="VUW50_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HET7983253.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSSWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSHEKLRAALHRMVPIEVKKKYKLRFKIKTDNKIGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVAAQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKGGTTNVIVSKDGKE
                     VKKIPLKILASVKDTYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKTSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTHRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKSPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            15718..16103
                     /gene="tnpA"
                     /locus_tag="VUW50_001369"
                     /pseudo
     CDS             15718..16103
                     /gene="tnpA"
                     /locus_tag="VUW50_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(16482..17111)
                     /locus_tag="VUW50_001370"
     CDS             complement(16482..17111)
                     /locus_tag="VUW50_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET7983254.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(17121..18122)
                     /locus_tag="VUW50_001371"
     CDS             complement(17121..18122)
                     /locus_tag="VUW50_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET7983255.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKDAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(18153..18794)
                     /locus_tag="VUW50_001372"
     CDS             complement(18153..18794)
                     /locus_tag="VUW50_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HET7983256.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(18813..19628)
                     /locus_tag="VUW50_001373"
     CDS             complement(18813..19628)
                     /locus_tag="VUW50_001373"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HET7983257.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            19899..20333
                     /locus_tag="VUW50_001374"
     CDS             19899..20333
                     /locus_tag="VUW50_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7983258.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPVKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            20345..21535
                     /locus_tag="VUW50_001375"
     CDS             20345..21535
                     /locus_tag="VUW50_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HET7983259.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            21546..22037
                     /locus_tag="VUW50_001376"
     CDS             21546..22037
                     /locus_tag="VUW50_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET7983260.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            22052..22831
                     /locus_tag="VUW50_001377"
     CDS             22052..22831
                     /locus_tag="VUW50_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET7983261.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            22818..23636
                     /locus_tag="VUW50_001378"
     CDS             22818..23636
                     /locus_tag="VUW50_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET7983262.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            23636..23929
                     /locus_tag="VUW50_001379"
     CDS             23636..23929
                     /locus_tag="VUW50_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET7983263.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            23951..25852
                     /locus_tag="VUW50_001380"
     CDS             23951..25852
                     /locus_tag="VUW50_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET7983264.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKFMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGMHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            25912..26913
                     /locus_tag="VUW50_001381"
     CDS             25912..26913
                     /locus_tag="VUW50_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7983265.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(27023..27358)
                     /locus_tag="VUW50_001382"
     CDS             complement(27023..27358)
                     /locus_tag="VUW50_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983266.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(27369..27611)
                     /locus_tag="VUW50_001383"
     CDS             complement(27369..27611)
                     /locus_tag="VUW50_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983267.1"
                     /translation="MKKEKRQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(27622..28164)
                     /locus_tag="VUW50_001384"
     CDS             complement(27622..28164)
                     /locus_tag="VUW50_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HET7983268.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIVLFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            complement(28180..28374)
                     /locus_tag="VUW50_001385"
     CDS             complement(28180..28374)
                     /locus_tag="VUW50_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7983269.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            28540..29490
                     /gene="rsmH"
                     /locus_tag="VUW50_001386"
     CDS             28540..29490
                     /gene="rsmH"
                     /locus_tag="VUW50_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET7983270.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            29502..29819
                     /gene="ftsL"
                     /locus_tag="VUW50_001387"
     CDS             29502..29819
                     /gene="ftsL"
                     /locus_tag="VUW50_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET7983271.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            29823..32075
                     /gene="pbpX"
                     /locus_tag="VUW50_001388"
     CDS             29823..32075
                     /gene="pbpX"
                     /locus_tag="VUW50_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HET7983272.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKVEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            32077..33057
                     /locus_tag="VUW50_001389"
     CDS             32077..33057
                     /locus_tag="VUW50_001389"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET7983273.1"
                     /translation="MFISLSAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            33621..35726
                     /locus_tag="VUW50_001390"
     CDS             33621..35726
                     /locus_tag="VUW50_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET7983274.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQEKVIGQDKAVEVVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGGLVIREKS"
BASE COUNT        11312 a         5970 c         7802 g        10834 t
ORIGIN      
        1 agacctagag aaatttttcg atacagttcc tcaagataga ttgatgtcct tagtacataa
       61 cattattgaa gacggagata cggaatcctt gattcgtaag tatcttcatt caggtgttat
      121 cattaacggt caacgtcata aaacgctagt tggcacacca cagggaggaa atttatctcc
      181 tctcttatcc aatgtcatgc ttaatgaatt ggacaaggag ttagaaaaga gagggcttcg
      241 atttgtgcgc tacgcagatg attgtgtggt tacggttgga agcgaggctg ccgctaagcg
      301 tgtgatgtat tcagtcagtc gttttattga gaaacggcta ggtttgaaag caaacatgac
      361 caagactaag attactagac taagagagtt gaaatatcta ggtttcgggt tttggaaatc
      421 atcagatggt tggaaaagtc gtccacatca agatagtgtt cggagattta agcttaaatt
      481 gaagaaacta acacatagga aatggagtat agacttagac agccgaattg agcaactaaa
      541 ctgggttatc cgaggttgga tgaactactt ttcaatgacg aacatgaaat cagtcatgga
      601 gagcattgat gaacgactga gaacacgaat aagagtgatt atctggaagc aatggaagaa
      661 gaaatcgaga cgattatggg gattgcttag gttaggggtt cctaaatgga tagcagataa
      721 ggtatctggc tgggcgacca ttatcaatta gtagctcaga agtcggtact taaacgtgct
      781 atgtcaaaac cagtccttga aaaacgtgga ttggtttcgt gtttggatta ttaccttgaa
      841 cgacatgcgt taaaagttag ttgaaccgcc gtgtgccgaa cggcacgtac ggtggtgtga
      901 gaaggactag agattatccc ctactcgata atttgaagga taattttgga ggatatgaga
      961 tgacattgac tattcatgta tattataaag gtgatggaga tagtgcaata aaatttgcaa
     1021 gagaaatgct tgatagtggc cttgttgaag aaattagaaa tcaaaaaggt aacttgaaat
     1081 acgaatattt tttaccaatt gaaaaagagg gaactattct ccttattgat cagtggataa
     1141 atcaagaaac tttagataaa cattatcaat caaagacaat gcagaaaatc ttagatttaa
     1201 gaaaaaaata tcacttgcag atgcaagttg aacgttatat agaagatgat tctgggatgc
     1261 cagaaagcga taagtcgttt atagacacag ggaattagaa ttattgtgag agtttttgta
     1321 gtttgaaagt attgaaaatc gtaaagatat cattacgaga atacaagaag atattgttct
     1381 atttccatta aaaattactt ctttccttaa caataaggaa aaagaatgcg ttttcatctt
     1441 ttggtttaag gtttataata agagtatcaa agtcaggagg aaaaaagatg actactttta
     1501 aagatagatt tttatggggt ggtgctgttg ctgctcatca acttgaaggc ggatggcaag
     1561 aaggtggcaa aggaattagt gttgctgatg ttatgactgc tggtcgtcat ggagtagctc
     1621 gtgaaattac ttcgggagtt ttagagggta aatattatcc aaatcatgag gcgatagatt
     1681 tttatcatcg ttataaagaa gacatagcac tttttgctga aatgggattc aagtgcttcc
     1741 gtacctctct tgcatggaca cgtatctttc caaaaggtga tgagttagag ccgaatgaag
     1801 aaggattaca gttttatgat aatctttttg atgaatgctt aaagaatggt attgaacctg
     1861 tcatcactct atctcatttt gaaatgcctt atcacttagt gaccgaatat ggtggttgga
     1921 aaaataggaa attgattgat ttctttgctc gttttgcaga agtcgtattt aaacgttaca
     1981 aagataaggt taaatattgg atgactttca atgaaatcaa taatcaagcg aattatcagg
     2041 aagattttgc accatttact aactcaggta ttgtatatga agaaggtgat aatagagagg
     2101 caattatgta tcaagcagca cattacgaat tagttgcttc tgcacgagct gtaaaaattg
     2161 gtcatgagat taatccagat tttcaaatag gttgtatgat tgcgatgtgt ccaatttatc
     2221 cagctacttg caatcctaag gatatcttaa tggcaatgaa agctatgcaa aagcgttatt
     2281 attttgctga tgtgcatgtt ttaggtaaat atcctgagca tattttcaag tattgggaac
     2341 gaaaaggtat ttcagttgat tttactgccc aggataaaga agatttactt ggtgggactg
     2401 tagattacat tggtttcagt tactatatgt cctttgctat cgactctcat cgtgaaaata
     2461 atccttattt tgattatctt gaaacagaag atttagtgaa aaataattat gttaaggctt
     2521 ctgaatggga gtggcaaatt gatccagaag gtttgcgtta tgcgttaaat tggtttacag
     2581 accactatca cttaccactc tttattgttg aaaatggttt tggagctata gatcaagttg
     2641 cagcagatgg tatggtacat gatgattata gaattgaata tctaggtgcc catattcgtg
     2701 aaatgaaaaa ggctgtagtt gaagatggtg ttgatttaat gggttatact ccatggggat
     2761 gtattgattt ggtttcagct ggtaccggtg aaatgcggaa acgttatggc tttatttatg
     2821 tagataaaga tgataatggg aagggaagtt ataatcgttc cccgaaaaaa tcttttggct
     2881 ggtataagga agttatttca tctaacggtg aatcagtaga atagtataaa caaactgaga
     2941 ttattctcag tttgtttagc atattgaggt gacatatgat tggaagtatt gcagcaattt
     3001 taacaacatt tgcattttta ccacaagttt ttcgtgtagt taagacaaag gacacaggat
     3061 ctattgctct aggtatgtat gttatgcaag ttattggtat tgcattgtgg cttgctcatg
     3121 gtattcgtat aggagacttg cctttgattt tagcaaatag tgtttcattt ctactctcag
     3181 gaataatttt attttataaa ttgaagtata aataattttt ttccatatca tttaggaaaa
     3241 tgagggaatt gtcattggaa acgctttatt ttataataaa aatagataac aaataagcca
     3301 acttagcaca gctttgattg gatttaagaa aaggagaaaa tattatggca aaagctttaa
     3361 ttatttgtgc agcaggaatg tcttcatcac ttatggcaca gaaaacaaca gagtttctaa
     3421 aaggaaaagg gaaagatatt gtagttgatg ctattagtgc tactgaagga gggaaagcta
     3481 tcgcagcagc agcttttgat ttgtatcttg tcagccctca aacaaaaatg tactttaagc
     3541 aatttgaaga ggctggttct aaagcaggaa aaccagttgt acaaatacct cctcaagcct
     3601 atattcctat tccaatggga attgaaaaaa tggctaattt gattcttgag aatatttgat
     3661 gaacatatga aatgtcaata aatatttagg ttaagataaa ttgttttgag tttgatataa
     3721 actgatttaa gtgtataata tgcttcaata tatgaaaaca tacgttttag gagggaatga
     3781 atgtgttaaa cattaaagag aaagatatcc ttcaattttt aataaaaaat aaggaacgtt
     3841 ttgttacaag caaagaactg gcagaatatt tatcctgttc agatagaact gtccgtaatg
     3901 tactgaaatt aattgagaag acaatgatta ttcaaggagt ccggttgatt tcaaaacaag
     3961 gtcaagggta tcaaatattt tttgagaatc aaggcgctta ccaggagttt agacaaactt
     4021 atgaactgga agaagactat acgaaaactg cggtttcaaa aggtgatgat cgtttagtat
     4081 ttatcttaaa taaattatta tttgagcaag ttccagttct ttttgatgat ttagctgatg
     4141 aactttacgt tagccgttca acattatctc atgatttcag aaagatacga gtgatgttat
     4201 cagagtataa tttgtccatt gagagtaggg cgaataaagg agtttatgtc tcaggcgagg
     4261 aacgtgataa acgacgtttt atcataaatt atttcctaga gaatcaattt tttaaaacaa
     4321 tacattgcta tgtaaaattt aacttttttg atcaaactgt tccattagaa gaatttgcta
     4381 gaattgtttt ggatgaatgc caagaggcca acctaaaact atctgatttt gtactacaga
     4441 atttagtagt tcacatagca ttgtctgtga ttcgtttaaa atccggattt gaaataaaaa
     4501 atatagactg tcagatgact gatgatgcaa tagagagaaa ggttgcccaa agaattcttt
     4561 ctaaagtaag agaagtaaca aatcaggaat ttccagttca ggaaatagac tacattactt
     4621 tgcatttgtt agctaagagt caacaatgtc aaaaaaatca gaagaatatt tctgaagaag
     4681 ttttaaaaaa atctttattt aaaacatttc agaatttagg attagatgat atgtataact
     4741 tttcttcaga tttccaacta attgaaggat tgataactca tctaatgaca ctacaagtac
     4801 gtttagaaag ccggattacc ttaaataacc ctttggtaga tgaaattaaa cagaattata
     4861 gtgatatatt ctttatgact agagaaattc ttgcaaatat ggacaagttc ttggagtgga
     4921 caatatcaga tgatgaagta gcatatgtaa gtcttcactt tttagctgct atggaacgta
     4981 gcaaagagag tactaagttt aatattcttg ctatttgtgc aacaggattt ggtgcagccc
     5041 aaatgttaag aaatcgtttg gaaacggagt ttgggaagcg agtagaagtt gttgatgtaa
     5101 taggctacta tgaattgaat caagaaaaac ttaaaggaat agattttata gtgtcagctg
     5161 ttgatttatc aaatctatat tttcaaatcc cagtttttaa agttagtgtg tttcttaaaa
     5221 gtgatgagat ggaaatgatt agaaaagcta tggatcagat gcaagtttct agttatgtac
     5281 aatcatcaaa gattaacaaa tttgaaaaca acggctttag acagtatttt agtaaagaaa
     5341 attttttaat atgtactgaa tcagacaagg taaatttact tgaaaagatg gttgagggtt
     5401 tatctgttgg tgaatcaaat gagtttgaac aatctttact ttatggaatt aaacagcgcg
     5461 aagaattaag ttcagttgtt ttttctgaaa aaattgcagt tccgcatcct attcagccct
     5521 ttggtacaga gggaaaggtt tcggttgcaa tttgtaaaga ttcgctactc tgggataatc
     5581 aaagttcaca tgtccagcta gtatttcttc tatctccatc aatatatggg aacgaaggtt
     5641 tggcaactgt gactaaaaag atagtctctc tgacagagaa tgatgaattg caaaatcagc
     5701 taatatcttg caataacttt gaagatttta ttaacatatt tgagaagata aaataggagg
     5761 ttcatatgaa tcaagaggaa ctgcaggttg cagcatttga aattatcctg cattcgggaa
     5821 atgcaaggtc agaaatacat gaagcatttg caaaaatgag agaaggtagt tttgatgatg
     5881 cagaaagtaa attaaatcaa tcaaatgaga taattcttga agcgcatcat gcacaaacca
     5941 aactcttgca agaatacgca agtggagtgg aaattaaaat tgaaattatc atggtacatg
     6001 ctcaagatca tttaatgacc actatgacat tattagaagt agcaaaggaa atgttagctc
     6061 tttataagaa agttaactaa atatgtgttg ttatataagg aggaaatgat atggataaaa
     6121 aagaacgaca aaagattgaa caacaacgtc gagagatggc tctaactaat acttttttca
     6181 atcgatatct tcttctacgt tattcaatag cacccttctt ttttgggaat atttattggc
     6241 ttttgaatca gtttattagt ccctcgccta ttattatttt ccccattatg ctcatagtat
     6301 tttctatttt agcaactgtt gaacaattta aactctatgg aaataggaaa gaaaagttgg
     6361 gaataacact aatgtttgtt agaatacaaa tgcttatttc aataggatta cttgttttaa
     6421 cttggacaag ttggtttaag aatctttttc cgatttttga aaataaccaa gtagcacgtt
     6481 tatttgtttt tgtagttctt ctattaggtt tagttctgag tttgttagac attagaagaa
     6541 ttaaaaaaat ttataaacga acagataagg cttatcaaca atttgtccaa ttggaaaaga
     6601 actcacttag tttataatcc tgatctagta taatttgtat ttgaaactag tctttagaaa
     6661 gtaaaaatta ttatgtttta ttaaggaggt ctatttatgg atcaacaaaa cgggttgttt
     6721 ggttttcttg aaaaccatgt tatgggacca atgggcaaac ttgctcagtt taaagtagtg
     6781 cgtgctatca cggctgcagg tatggctgct gtaccattta ctattgtagg atcaatgttt
     6841 ttggtattca gtattttgcc acaagctttc tcattttggc caattgtggc agatattttc
     6901 tctgcttcat ttgataaatt cacatcactt tacatggttg caaactatgc gactatgggt
     6961 tctctatctc tttatttcgt tctatcactt gcatatgaat tgacaaaaat ttatgcagag
     7021 gaagaagaac tcaatatgaa tcctcttaat ggtgccttgc tcgccttgat ggcttttgtc
     7081 atgacagtac cgcaaatcat ttttgatggt ggaatgatga agactgtgac aagtctaaaa
     7141 gaaggtgcag taattgcaga tggatgggca atgggaaatg gagtcgcacg ttttgggaca
     7201 acagggattt ttaccgcaat cattatggca attgtgactg ttcttattta tcgtatgtgt
     7261 gttaaacata attgggttat taaaatgcct gaagctgttc cagaaggagt ttctcgtgga
     7321 tttaccgctt tggttccggg atttgttgtt gcatttgttg ttatctttat caacggtctt
     7381 cttgtagcaa tgggaacaga tatttttaaa gtcattgcaa ttccatttgg ttttgtatcc
     7441 aatctgacta attcgtggat tggtttaatg attatttatc tattgactca actactttgg
     7501 attgtaggta tccacggtgc gaacattgtt tttgcatttg ttagtccaat tgctcttgct
     7561 aacatggctg aaaatgctgc tggcgggcac ttcgctgttg caggtgaatt ttctaatatg
     7621 tttgtaattg caggtggttc tggtgcaact ttaggactat gtttatatat tgcttttgcc
     7681 tctaaatctg aacagcttaa agcaatagga cgagcatctg tagttccagc cttatttaat
     7741 attaatgaac cattaatttt tggattacct attatctata atccagcctt ggctatacca
     7801 tttattttag caccaatggt tactgctact atttattacg tagcgaattc tctaaacttt
     7861 attaagccaa ttatcgcaca ggttccatgg ccaactccag tagggattgg agctttctta
     7921 gggacagcag atcttcgagc tgtattagtt gctctagtat gtgcatttgc agcattccta
     7981 gtctatcttc cattcatccg tgtatatgat caaaaattgg tgaaagaaga gcaaggtatc
     8041 taataagtaa aaaagtatac tcgatgatag aatccatata gtttaaataa tgagtgttaa
     8101 cattagcagt gaagaggtct ttttaagata ttcttctctg ctttttcata ttttttcgct
     8161 atgtagggta tgaagcaaaa tagaaagtag ttcgaatagt tgataaacag ggaactgctg
     8221 ttatttaaaa aaacaataaa gtaaagtatg ttatcgtaga atacgataca atagtaaaaa
     8281 agcaaaaaga atgcgtttta tatttgggtt tgaggcttat aaaataggat aagcgtgctg
     8341 aaaacttaga tatgacaaac aaaataaaat ttttaaaagc tacagagaaa catggtataa
     8401 tgtatctaaa ggaggtagca tatgtcctta gatatagata aagaaaagat gacaatcatg
     8461 ggaatagctt ttgaaaaccg atccgttttt aagagtgttt ggtatgcttt aagtacaaat
     8521 atgattgaag gatggcgacc tacggttagt gatgttgaaa aattacgaga tgaagctctt
     8581 tctctgggga tgacttaatg aagcgcaact gttatgagtc ttatgactat gttgatccgg
     8641 ataatttata taccgatccg ggttcttcag ttctaagaaa taagcaggaa ggaaaaaagc
     8701 acgcgagctt gaatatcgta tgattgctag taagagtttg aaattattta tcaatcctat
     8761 cttagttaat agtttaaaag tagtttgttt cacaactgca taagcttaga aaagttgaaa
     8821 agtgataact ctttaataaa ctcttggttt gttaaaagat attttgtcca ctacattttt
     8881 ttcaattgac tattatttgt catagatata acagatagaa cattgcattc agaataatga
     8941 aaaacgcatc aaaaatggtg cgttctgttt ttatgctacc ataaaaggag gaaaacaaat
     9001 tgaaaatttt taagggagag ttttatcgaa tctctgtatt aacagacaag ctagtaaggt
     9061 tagaatactc tcgaactgga aattttgagg atagaacgac acaacttatc tataatagag
     9121 attttggcca agtttcgtta gattatatcg agacatcaaa tgtactagat attatgacgg
     9181 actattttca tctgcacttt aataaaggag aatttaacgc cgaaaattta tttatagaat
     9241 taaaaggaaa ttttgccgta tatggtagtc gctggtattt tggtgaatct attgaaacgt
     9301 taaaaggaac agctcggact ctggataagg cagatggagc aatctcgtta gaagatggaa
     9361 ttattagccg aaatggtata gccttattgg atgattctca aggatttatt tgggatgaac
     9421 aatctggtta tattgagaga gaaaatcaaa ttgacctgta tttctttgcc tatgggcatg
     9481 attatagagg agcaatcaga gacttttacc atttgactgg ttcaacaccc ttgttgccaa
     9541 gatatgcttt aggtaattgg tggagtagat attggcctta tacgtcggat gaatacttgg
     9601 atttaataga cagatttgaa acagagaaaa ttccattatc tatcggtgtg ttagatatgg
     9661 attggcatat aactgacatt ccagctcgtt ttggaagtgg ctggacagga tatagttgga
     9721 atagaaactt aataccaaat ccagaacagt tattgcaaca acttcatgat agaaagttaa
     9781 aactctcctt aaatgtccat cctgctgatg ggatacgggc ttatgaagaa gcttatcctc
     9841 aaatcgcaaa acgattgggg ttaaatgtag aactagaaga acctgctatt tttgattttt
     9901 ttaatccctc ttttagggaa gcctacttta aagatgttca ttatgagcta gaaaagcagg
     9961 gagtagattt ttggtggatt gactggcaac aagggacaca aggtatgcta gatccacttt
    10021 ggcttttaaa ccattatcac tatcaggata gttgtaaaaa tgcagaaggt ggtttgattt
    10081 tatcaagata tgcaggtcct ggtagtcacc gctaccctgt tggtttttca ggggatacta
    10141 ttattagttg gaattcctta agatttcaac cctattttac agcgacagca tctaatatcg
    10201 gttatagttg gtggagtcat gatatcggtg gacatatgtt gggggattat gacgaagagc
    10261 tacaaactag atggctacag tttggtgttt ttagtccgat tactcgatta catagttcta
    10321 gaagtccttt taatagtaaa gaaccttggt ttttttcaga aacaacatct aagattatga
    10381 agaaatacct tcgtttgaga catcagatga ttccctatct atataccatg aatgtaaaga
    10441 cacatgagga aggtgcccca ttaatcagtc caatctatta tttctatcca gagaatgatg
    10501 agagctataa tgttccaaac caatactttt ttggaacaga actgatggtg gctcccattg
    10561 tagaaaagat ggatttgaca ttccaatctg caaaagtaga tgtatggttc cctgaaggtg
    10621 aatggtatga cttcttttca gagaaaaaat acacaggtgg tgtgaagtta agtgtttata
    10681 gggacatctc gactacgcct gtgtttgcaa aaagtggtgc aatcattccc ttggttggtt
    10741 ctgagatagg tatgggtgtt gatttacctg aagttgtaga ttggtatgta ttcccaggga
    10801 aacaacattc ttttgaaatg cttgaagatc aaaatggtca aagatataaa acaagattat
    10861 caatcgattg ggaaatggga atggtagagt tagcattaca aggagattct agtatcgttc
    10921 caagcaatag aaaacataga attcatttta aaggaacgaa tgtgtctata attgaattgc
    10981 caaataagaa tgatacagct aaatttgaat ggaaagataa taaaaggaca tctttaaatg
    11041 acgaagtttt tagactacta aagacagctt ctcttccata tgaattaaaa gatagattgt
    11101 taaatcaatt catcaatgcc aaaaactctc atgacttaat gaatatcttg catcatcagg
    11161 ataaggaatt gagagggcgt ttgttggaaa tgatatttac tagccaaaac taattgaaat
    11221 aattagaaag tcatttgttt agtgtatatg aagattctgt atggtattcg ttatcactgg
    11281 tcacattgaa attgtgaaat aagcacctac aacatagact ttacaacact tcatgtttca
    11341 tatcatgggg tgtttttcta tttaagtcaa agccaatcaa aaaatctcac tattcatcag
    11401 gttaaaggaa acctaatcat agtgagattg tgaattatag tagagtttgt atcgcctctt
    11461 caatttgttt tgggtctgtt tttgaagaaa agcgttcaaa gacttgccca tctcgaccga
    11521 tgagaaactt agcgaaattc cattcgactc gttttcctag tgggccagat ttctggtctt
    11581 ttaaccagac atagagaggg tctgcttcct taccgttgac cttaatcttg gcaaaacgtg
    11641 ggaaggtggt ttgaaaatgt aggctacaga aggcgttgat ttcctctgcg ctgccgggtg
    11701 cttgtcccat aaactgattg caagggaaat ccaatatttc gaagccctga tcttgatagc
    11761 gttcatagag ttcttgaagt ccctggtact ggggcgttaa accacatcca gtagcagtgt
    11821 tgacaatcaa gagaactttg ccacgatagc tatccaaggg agttgcttgg ttgttttggt
    11881 tcaaaacgga aaaatcataa agtgaagtca tgagggcctc ttttcttttt ctacattgta
    11941 acagttttta cttttttcgt cgagtcggat ggaagatatt gttgaagaat aagccctcaa
    12001 tatgatagaa atcgtttaca aataacttta aaaatggtat gatttaacta aaccggttta
    12061 cttagttgct ataaatctct aatttgagat ttagttccta ttgattttac aatatgttta
    12121 ttggagtgta tacatgcaaa caaaaacaaa gaagctcatt gtgagtttgt cttcacttgt
    12181 tttatcagga tttttattaa accattatat gacagttgga gcggaagaaa cgactacgaa
    12241 taccattcag caaagccaga aggaagttca gtatcagcaa agggatacaa aaaatttagt
    12301 tgaaaatggt gattttggtc agacggagga cggaagcagt tcgtggacag gaagcaaagc
    12361 tcaggggtgg tcagcttggg tagaccagaa gaatagttct gcagatgcct caactcgagt
    12421 cattgaggct aaggatgggg ctatcactat ctcaagccat gagaaattaa gggcagcgct
    12481 tcaccgtatg gttcctattg aagttaagaa aaagtataaa ctgcgtttca agattaaaac
    12541 agataataaa atcgggattg ccaaagttcg tatcattgag gaaagtggta aggacaagcg
    12601 attgtggaat tctgcaacga cgtcaggaac aaaggactgg cagaccattg aagcagacta
    12661 tagcccgact ttagatgttg ataaaatcaa gctggagtta ttctatgaaa caggaactgg
    12721 gactgtttcc tttaaggata ttgagctggt agaggtagca gcccagcttt ctgaggattc
    12781 tcaaacagat aaacagcttg aggaaaagat tgatttacca attggaaaaa aacatgtttt
    12841 ttctcttgcg gactatactt ataaggtaga aaatcctgac gttgcttcag tcaaaaatgg
    12901 aattttagaa cctcttaagg gagggacaac caatgtcatt gtcagtaaag atggcaagga
    12961 agtgaaaaag attcctttga agattctagc ctctgttaag gatacataca cagaccgttt
    13021 ggatgactgg aatggcatca ttgctgggaa tcaatactat gattctaaaa atgaacagat
    13081 ggccaaatta aaccaggaat tggaaggaaa ggtagctgat agcctatcca gtatttcaag
    13141 tcaggcggac cgcacctatt tgtgggaaaa attttcaaat tataaaacgt ctgcaaatct
    13201 gactgccact tatcggaaat tggaggagat ggccaagcaa gtgaccaatc cttcttctcg
    13261 ttattatcaa gatgaaactg tcgttcgaac agtcagggat tccatggaat ggatgcataa
    13321 acatgtctac aatagtgaaa agagcattgt tgggaactgg tgggattatg aaatcggtac
    13381 acatcgtgcc atcaacaata ccttgtctct gatgaaagaa tacttctctg atgaggaaat
    13441 taaaaaatat acagatgtga ttgaaaaatt tgtaccagat cccgaacatt tccgaaagac
    13501 gactgataac ccatttaagg ctctaggtgg aaacttagtt gatatgggaa gagtaaaagt
    13561 aatagctggt ttactgcgta aggatgatca agaaatttct tctaccattc gctcgattga
    13621 gcaagtgttc aagttggtag accaaggtga aggtttttat caagatggat cctatatcga
    13681 ccacaccaat gttgcctata cgggtgctta tgggaatgtt ttgattgatg gcctgtctca
    13741 actgttgcca gtcattcaaa agaccaagag tccaatcgat aaagataaaa tgcaaaccat
    13801 gtaccactgg attgataaat cgtttgctcc tttgctggtg aatggagagc tgatggatat
    13861 gagtcgtgga cgctcgatca gtcgtgcaaa tagcgagggg cacgtggccg cagtagaagt
    13921 actaagaggg attcaccgaa tagcggatat gtctgaagga gaaaccaaac aacgtttgca
    13981 gagtcttgtg aagaccattg ttcaatcgga tagttattat gatgtcttta agaatttgaa
    14041 gacttataag gatatcagtt tgatgcaatc cttgttaagt gatgcaggag ttgcaagtgt
    14101 tccaagaaca agttacctat ctgcctttaa caagatggat aaaacagcca tgtacaatgc
    14161 agagaaaggg tttggatttg gcttgtcact cttttccagt cgtaccttga attacgaaca
    14221 catgaacaag gaaaataaac gtggttggta tacgagtgat gggatgttct atctttacaa
    14281 tggcgatttg agtcactata gcgatggcta ctggccaaca gttaatccat ataagatgcc
    14341 tggtacaaca gagacggatg ctaagagagc ggatagcgat acaggtaaag ttctaccgtc
    14401 tgctttcgtt ggaacgagca aactagatga tgccaatgcg acagcaacca tggatttcac
    14461 caactggaat caaacattga ctgctcataa gagctggttt atgctgaagg ataagatcgc
    14521 ctttttagga agcaatatcc aaaacacttc aacagatact gctgcaacta caattgacca
    14581 gagaaaactg gaatcaagta atccatataa agtctatgtc aatgataaag aagcctccct
    14641 tacagaacaa gaaaaggatt atcctgaaac ccaaagtgtc tttttagaat cgtccgattc
    14701 gaaaaagaat attggttact ttttctttaa gaagagttca atcagtatga gtaaggcttt
    14761 gcaaaaggga gcctggaagg atatcaatga aggacagtca gacaaggaag ttgaaaatga
    14821 atttcttacg attagtcagg ctcataagca aaatggagat tcttatggct atatgctcat
    14881 tcctaacgtg gatcgtgcca ccttcaatca aatgataaaa gagttagaaa gtagcctcat
    14941 cgaaaataac gaaacccttc agtctgttta tgatgctaaa caaggagttt ggggtattgt
    15001 gaaatatgat gattctgtct ctactatttc caaccaattc caagttttga aacgtggagt
    15061 ctataccatt cgaaaagaag gggatgaata taagattgcc tactataatc ctgaaaccca
    15121 ggaatcagct ccagatcagg aagtctttaa aaagctagaa caagcagctc agccacaagt
    15181 acagaattca aaagaaaagg aaaaatctga agaggaaaag aaccattcgg atcaaaagaa
    15241 tctccctcag acaggagaag gtcagtcaat cttggcaagt ctagggttct tgctacttgg
    15301 ggcgttttat ttattccgta gaggaaagaa caactaattt gttcatcatc tagatgaatt
    15361 acatgaaact gtggaaaata gaaaaaatcc gaagaaggcc aaatatcttc ggatttttct
    15421 tttaaaaaag gataagttga tacttatcct tttttacttc attatgtgac tatagcgttt
    15481 tgaaactaga ataggacgtt ataactacta aaagatttct ataaattcat ttgattttcc
    15541 taatcaattt gttcgtatcc tatttcactc cactatatta gtccgtctcg ctccgtgagg
    15601 tgcgagacaa aacaaccacc cgctatacgg gtgcgcgtcg aaggttatac caaaaaaact
    15661 ccaaacgcga tacaataaag gtgttcaatc cacttgtaaa gcgaaaggag aaaaaatatg
    15721 gcacaaaagg ctcatagttt atctcacaca aagtggaggt gttctatcac attgtgttca
    15781 cccctaagta tagacgaaaa gtcatctata atcaatatag aagtagttta ggcgaaatat
    15841 ttcatcgctt gtgtagttat aaaggtgttg aaatgatcga gggtcactta atgccagacc
    15901 atgtacacat gttagtcagt attccgtcga ggctaagtgt ttcaagtttt atggggtatt
    15961 taaaaggcaa aagtgcactc atgatgtttg acaaacacgc caatctcaag tacaagtttg
    16021 ggaatcggca tttctgggcg gaaggttatt atgtaagtcc agtagggctt aatgaagcca
    16081 caattaagaa atatagtcaa gattaaagaa attatcgagt ggatgatttc ttcacgagta
    16141 tgaaaatttg agaactagta aagtatgata tatagtaaaa tgaaataaga acaggacaaa
    16201 atcgatcagg acagtcaaat cgatttttaa caatgtttta gaagtagagg tgtactattc
    16261 cagtttcaat ctactatagc actagataaa ttgagtgtaa aagaatatga ggatcccttt
    16321 agggatagtg gtaagtaata ccaaagtctc ttaaagaggc aagtgacgag tcaagagcaa
    16381 taaggcttga acaacgtgaa agccagcgtc tttaggcgct ggctgatgat ttgggattat
    16441 agctctgaga taaaccaccc gttagacggg tagttatgat tttatctgag tgtaatatac
    16501 tgttgggcaa tctcgctgat gcggtcaaag ttgccttggg aagcgagttt attgagttcg
    16561 ccaccaattc caacggcgtc tgcaccagca gcgaaccatt gagggatgtt gtttaggccg
    16621 actcctccgg ttaccattac ggaaacttgt gggatcggtg ccttgactgc agagatatat
    16681 gctggactga gagtactacc tgggaagagt ttgatgattt cactaccggc ttcaagtgca
    16741 gtcgtgatct ctgtgagggt aatacagcct ggaatgtacg gtgtgctgta gagattgcac
    16801 attttcgcag tttcagcatg gaaagatgga gaaacaacgt aatttgctcc agctagaatg
    16861 gcatctctag cagttacggc atcaagcaca gtacctgcac cgatacaaac actctgatcg
    16921 tcctgataca agtctacaag ttccttgatg atttgtcctg catactgatt ggtataggcg
    16981 atttcaatag ctttgatacc gcccttgata caagcaatcg aggcttgtag tccttcttcc
    17041 tttgtatttc ctcgaatgac agcgacaatt ttctgttttt ttagttcaat aatcgtatct
    17101 gatttggtca tgtaattctc ctaacgaatg atatcttgtg catttgccag taaattttca
    17161 atactagttg cggaagtgga gagatggtct cctggaagag tgcatttgag agttgcgctc
    17221 gcaactgcaa agtcaatggt agtttttagg gaggaatgat ggagtagttg gtaaagggca
    17281 ccagatataa aggcatcccc gctaccaatt cgttgataga ctgcagtttt tagttggaca
    17341 gactcttcaa atcgttcttc tagagcatag gcttgataga catttttgtc ttgctcatca
    17401 ctagagcgga gggtatggaa aatggccttg aaaccatagg cgtcttttaa aagtcgcatg
    17461 cgattttcca cctcttctag gctagcactg tctcttggaa acatctctag attttggtca
    17521 tcaatcatga gaggatcaat accgaagcaa tagtcagtaa aacgtgcaaa cttagaaaat
    17581 tcatacttgg cttctaggac tgaaatcatc tttgttctca gattgagatc cattgaaacg
    17641 acaattcctc ggcgcttggc ttcttccaag agtaggagaa ggatcgcacg gacctcttga
    17701 ccgatagcta cggtgattcc actaaaatga aaatggctaa tcccctgaaa gagagaatcc
    17761 atatctagca tgtttggccg aatctggctg atactcgtat gcttacgatc gtagaaaact
    17821 tcactttgac gacaaccaaa gccgttctcc aaatagtaga ggccgattcg atcgccaagc
    17881 cgacagattg aactggtatc gatttggtgc tgtttcaaga atgtgagaaa acgatctcca
    17941 atctcgttgg cagggagtgc ggtaaaaact ttcgttgaga tacccagggc ttgcaaatta
    18001 caagcgatgt taatttctga tccgccaaaa taagtcgaac tggcaacatg atcgccgata
    18061 ctggtggcat ctaatggtga aattcgaatt aatggttcgc caaacagtaa tattttcgtc
    18121 atccattagt tcctttctgg tttagaatag ctttacttgg ctaatacttc tttcaaataa
    18181 gcagcgactt catcatcttg gcagttttca aagaagtatt cttggaagta tttgccagag
    18241 atggtgtctt tgaggaagtc ttggtcgatt gttttaagga tggtcatcaa atcattgtgg
    18301 gtgatttgtt tcacctcgtt taagatacga gcgttgcgtt gttcagggat tacacgttct
    18361 cttgggtagc caccgcccat ttcttcagca aataagcgtt caaacatcaa tttgagggtc
    18421 agttctgccc cccagccaaa tcctttggca taagggatag acaaggcgtt accaccattg
    18481 atttgagaat aaaggtaagc gtcagttggg tccactgcta gaccacagac aacaccaggg
    18541 aagctgttta aagcaagcat agcccctaca cccgtaccac agccggtaac aacaaagtca
    18601 actgcctttg tatttaaaag gatggcagcc attagtccgt tctgcacata agttaattga
    18661 ctttctcctt cttctccacg cataccatag ttaaataatt ggtagccttt tttatctgtc
    18721 gcttctttta gactatcgta aataatgtga ttcttgctag cttgactatt ttcattgatt
    18781 aatgcgattt tcataagatc ttctttctat ttttactcag gttgttttcc gatgtaggct
    18841 aagataccgc catctacata taggatgtgg ccattgacaa aattgctggc atcactagcg
    18901 agaaagacag cagggcccat caaatcttca ggatttcccc aacgtgcagc aggtgttttt
    18961 gcaatgatga actggtcaaa tgggtggcga gaaccatctt cttgcaactc acgaagaggt
    19021 gctgtttgag gagtggcaat ataacccggt ccaattccgt tacattggat attggctcca
    19081 ccgtattcag acgcaatgtt gcgggtcaac attttcaagc cccctttagc agcagcataa
    19141 gcgctaactg tttcacgtcc cagttcgctc atcatcgaac aaatattgat aatctttcca
    19201 tgccctttct ttatcataga aggaataact gcctttgaaa cgataaatgg tgcgtttaag
    19261 tcaatatcga tgaccttacg gaaatcagcg gcgctcattt cgcacattgg aactcggcgg
    19321 ataataccag cgttattaac gaggatgtca atgacaccaa cctcttgttc gatttgcttg
    19381 accatggctt ggataccgtc ctcgtctgtc acgtcacaga catatccatg ggcttcgatg
    19441 ccaacttcac gataagcttc aatcccttta ttgaccagat cttgattgat atcgttaaag
    19501 acaatagtag cgccggcttc agcgtaggat ttggcaatag caaatccaat tccataagaa
    19561 gcgccggtga tgagagcaat ttttcctttt aaagaaaact gctcaattga gaatgatgta
    19621 tttgtcattt gatacttcct ttcaaatttg ttaagacttc tctataacca aattatattc
    19681 tagtaaaccg gttttgtcaa taaataaata tttttattta taaaaagatt tattttcctt
    19741 aaaaatggaa tattttgaaa tttctgccta ttaaaatgat taaaaagata atgaaagcgc
    19801 taatattttt ttaaaaaaac tattgacaag ataaaaataa aggattacaa tggggaatat
    19861 aaagtaaacc ggtaaaccta aacaagaaag gagaaaagat gaaaattgta cttgtagggc
    19921 atggacattt tgctacaggg atttatagtt ctttacaatt gattgcaggt aatcaagaaa
    19981 atgtggaggc gattgacttt gtggaaggaa tgtcagcaga tgaactcaag caaaaaatct
    20041 tacttgcaat ttcaaatgaa gaagaagttt taatcctaag tgatctcttg ggaggatcgc
    20101 cattcaaggt ttcttctacc ataatgggag aaaatccagt caaaacaatg aatgttctct
    20161 cgggtttgaa cttagccatg ttaatggaag cagtctttgc tagaatggct catagctttg
    20221 atgaggttgt taataaatca gtagtggcgg cccagggcgg agtcgtaaat ggtaaagaat
    20281 tgttttcaac ggatgcagag gaagaggaag aagatttcga atcgggtatt taaagggtaa
    20341 aagaatgata aaaaaggtta cgattgaaaa aataaaatcg cctgagcgct tcttagaagt
    20401 accacttctg acgaaagaag aagtcggcca ggcaatcgat aaggttattc ggcagttaga
    20461 actcaacctt gactatttca aggaagattt cccgacgcca gctacctttg ataatgtcta
    20521 tccaatcatg gataacacgg aatggaccaa tggtttctgg acaggagaac tgtggttggc
    20581 ttatgaatac agtcaacagg atgcatttaa aaacatcgct cataaaaatg ttctttcttt
    20641 cctggatcgt gtcaataaga gagtagaatt ggatcaccat gatctcggct tcttgtacac
    20701 accgtcttgt atggctgaat ataagataaa tggagatgga gaggctagag aagcaacctt
    20761 gaaagctgca gataagttga ttgaacgcta tcaagaaaaa ggtggtttta ttcaagcttg
    20821 gggagacttg ggcaagaaag agcattaccg tttgattatc gactgcttgc tcaatatcca
    20881 actcttattc tttgcttatc aagaaacagg cgatcaaaaa tactacgata ttgcagaaag
    20941 ccatttctat gcttcagcta ataatgtaat ccgtgatgac gcttcgtcct tccacacctt
    21001 ctattttgat cctgagacag gtcaaccctt taaaggtgta acgagacaag ggtatagtga
    21061 tgattcatgc tgggcacgtg gtcaatcatg gggagtctat ggtattcctt tgacttatcg
    21121 tcacttaaaa gacgagtcct gctttgactt gtttaagggt gtgaccaatt atttcttgaa
    21181 tcgtctgcca aaagatcatg tgtcctattg ggatttgatt tttaatgatg gtagtgctca
    21241 atcacgagat tcttcagcaa cagctatcgc cgtctgtggg attcatgaaa tgctaaaaca
    21301 tctcccagag gtggatgctg acaaagatat ttataaacat gctatgcatg ccatgcttcg
    21361 ttccttgatc gaacattatg caaatgatca atttacccct ggtgggacaa gtctcctcca
    21421 cggtgtgtac tcatggcatt caggtaaagg agtggatgaa ggcaatatct ggggtgacta
    21481 ctattaccta gaagccctta tccgtttcta caaagactgg aacctatatt ggtaggagga
    21541 gaaatatgac aatgccaaat attattatga cccgtatcga tgaacggttg attcatggac
    21601 aaggacaact ttgggtaaaa tacctaggtt gtaatacggt cattgttgcc aatgacgaag
    21661 taagcacgga caagatgcaa caaactctga tgaaaacagt tgtgccagac tcagttgcca
    21721 tgcgtttctt ccctttgcaa aaggtgattg atatcattca caaggctaat cctgctcaaa
    21781 cgatctttat cgttgtaaag gatgtgaagg atgctttaac cttggtagaa ggtggtgtcc
    21841 ctatcaaaga aatcaatatt gggaacattc acaatgctac tggtaaagag caagtgacac
    21901 gctccatctt cctgggtgaa gaggacaagg cggccctcaa ggaattgagc caaactcatc
    21961 aagtaacatt taatacgaaa acaactccaa caggaaatga tggagctgtt caagtcaaca
    22021 ttatggacta tatttaaaag aggagatcgt tatgtcgatt aatgtatttc aagcgatttt
    22081 aattggatta tggacagctt tctgttttag tggaatgctg ttaggaattt acaccaatag
    22141 atgtattgtt ctgtcatttg gtgtcggaat tattctaggt gatctgccta ctgctcttgc
    22201 aatgggagct attggtgaat tggcttatat gggattcggt gttggtgctg gaggtactgt
    22261 tccaccaaac ccaatcggac ctggtatctt tggtaccttg atggctatca ctagtgctgg
    22321 taaagtcagt ccagaagcgg ctcttgccct ctctactccg attgctgtgg cgattcaatt
    22381 cttacaaact ttcgcctaca ctgtacgtgc tggtgcgcct gaaacggcga tgaagcactt
    22441 gaaaaaccat aatttgaaga aatttaagtt cactctaaat gcaacaattt ggttgtttgc
    22501 ctttattgga tttaccttgg gttgcttggg tgccctttca atggatacct tgttgaaact
    22561 cgtagactac attccaccgg tattacttac aggtttgaca gttgctggta aaatgctccc
    22621 agctatcggt tttgcgatga tcttgtcagt gatggctaag aaagagttga ttccctttgt
    22681 cttgttggga tatgtttgtg cagcttatct aaacatccca acaattggta ttgcaattgt
    22741 aggtactatc tttgctttga ttgaatttta taacaagcca aaaacagcgg atcatgtggt
    22801 agaggaggaa gcacacgatg actggatcta acaaattaac aaaacgtgat tatcttaaaa
    22861 cgtctttgcg ggcattcttt tgtcaaaatg gatttaacta tagtaactat caagggttgg
    22921 gatatgccaa tgtgatgtat cctgctttga aaaaacacta tggagaggat caggaaggtt
    22981 tctaccaagc cttggaagaa aactgtgaat tctataatac caacccacac ttcctgcctt
    23041 ttattaccag cttgcatctt gtaatgttgg aaaatggtcg accaacaaaa gaaacacgta
    23101 gcatcaagat ggccttgatg ggaccattgg caggtattgg ggattctctt tctcaattct
    23161 gtttagctcc attgttctca accatcgcag cttcgtttgc tcaagaaggc ttggttgtcg
    23221 gtccaatctt gttcttcctt gcgatgaata cgattttaac agcgattaaa ttgtcaactg
    23281 gtctgtatgg atacaaacta ggaacaactg tgattgataa actaagcgag cagatggcaa
    23341 cgatttctcg tattgccaat attatcggtg taaccgtaat tgctggtttg gcagcgacat
    23401 ctgttaagat tatggtgccg attacctttg ctgcagggga agttaaagca gacgctaaac
    23461 aaagtatcgt aagtattcag ggaatgcttg ataaggttgc tccagctctt ctaccagccc
    23521 tatttacact tttagtttac tacttgatca aagaaaagaa atggacaaca tataaactcg
    23581 ttattttaac agttatcatc ggaattatcg gaagctggct taagattata gcttaatgga
    23641 tacaacattg ttttatggaa tagtgattgt cttggcagtg agtccccttt tactgtcaag
    23701 ctttcattct attcgtcagc aaaagttgct tcgcaaacag atggagcaac gacaagagta
    23761 tttagcttct ttaacatctg gtgatgaagt gttgttattg tctggaattc atggaaaaat
    23821 catttctatc aaagatgact tggtctcctt gcagattgca aaaggtgtgg tcatctatgt
    23881 agaaaaggaa agtgtaatgg gaaagacaaa agaactgctt tttaagtagt tctttttctg
    23941 gtaatagata atgaaagaag aaagaagaca attttttgaa agagtcgatg gaaaccaatg
    24001 tcgtgattat atcttgtccc actgttcaaa agactatgag aaagtcaaga gttcccttga
    24061 acgcttgatg gacaatcgtt ttatgtttga tagtccttgg gatatggagc cttgttcaaa
    24121 aatccatcaa atccagccga tggtatggga tcaagtattt gaagatgatc cagaatggtc
    24181 ttatatgctc aatcgacaag aatatctctt gcagtttatg atagggtatc tggtagaagg
    24241 agataaggac tatattcaaa agtgcaagtt ctttctattt gattggattg agcaggtgag
    24301 agaattttct cctcaatcct tgatgactag aaccttggat acgggtattc gttcctttac
    24361 ttggttgaaa ctactcttgc tcctcttgaa atttgacttg ctagaggaga aagaactaga
    24421 gaaaattttg gtcagtctag aaaagcagat tgactttatg aaaagccact atcgcgccaa
    24481 gtacaccctt agtaactggg ggattttaca aacaattccg atgcttgcta tctatcattt
    24541 cttttcagat aagatggacc tagaagaagc ttaccatttt gcttcagagg aattgaaaca
    24601 gcaaattgag acacagattt taggagatgg aagccagttt gaacagtcga ttctctatca
    24661 tgtagaggtt tataaagcct tgctggattt gtgtctcttg cttccagact tgcaagatag
    24721 ttaccaagag ttgctggaaa agatggcgac ctatattcaa atgatgacag gcttagatgg
    24781 acggactttg gcttttggtg atagcgattc tacagaaacg acagaaattt tgagcctgtc
    24841 tgctgtggtt ttgaacaagg aagaccttct taacggtctg gatgttaaag ttgatttgct
    24901 tagcctcttg ttcctggggc gagaaaaggt caagcgactg caggaatttg aaaagagagc
    24961 ttggcagcct aagttcatga tctttgaaga ctctggacat gtctgcatta aggatgaaca
    25021 tcgttatcta tttttcaaaa atggtccgct aggaagtgcc catagccata gtgacgagaa
    25081 tagtttttgc ttacagtatc aaggccaacc tattttcata gatgctgggc gttattctta
    25141 tcgggagata tatgaacgtt atctcttaaa gagtgcttgg agtcattcga cctgcattgt
    25201 agatgggaaa gctccggaaa gaatcacggg atcctgggaa tatgaatact atcctcactc
    25261 cctgttttgt caccataaag aaagggaggg aatgcattat attgaagggg cttattggtc
    25321 agcagaacct gatttgcctt atcttcacaa gagaaaaatc ctcatgttgg tagaggatgt
    25381 ctggctcttg gtagatgaca tcaggtgtca aggtcagcat gaggcgttga ctcagtttat
    25441 ccttgacaag gatgtgacct atcaagatgg gaaaatcaat cagttgagac tatggagtga
    25501 agttgatttt gatttggaag ataccatcat ttctcctaaa tacaatgagc ttgaaagaag
    25561 tagcaaactc accaagcgcc aattctttga gaatcagatg ctggattata ccatcattgc
    25621 gcatgagagt tttgaaatca tccgtcattc tgtctaccag acagatgatc gtgaagtgga
    25681 aaatgctctg gcttttgaag tgaaaaatga cgaaacagac aagctgattc tgttattaag
    25741 cgaggatatt ggtgtaggtg aaaaattgtg cctcgttgac ggaacaaaaa tgcgtggaaa
    25801 atgtctagta tatgataaaa taaatgagag aatgattcgc ttgcagtgct agaaataggc
    25861 attttgaata gtgaatatgt tataataagt attagtagga ggtgttttag attggagaag
    25921 aaactgacca taaaagacat tgcggaaatg gctcagacct cgaaaacaac cgtgtcattt
    25981 tacctaaacg ggaaatatga aaaaatgtcc caagagacac gtgaaaagat tgaaaaagtt
    26041 attcatgaaa caaattacaa accgagcatt gttgcgcgta gcttaaactc caaacgaaca
    26101 aaattaatcg gtgttttgat cggtgatatt accaacagtt tctcaaacca aattgttaag
    26161 ggaattgagg atatcgccag ccagaatggc taccaggtaa tgataggaaa tagtaattac
    26221 agccaagaga gtgaggaccg gtatattgaa agcatgcttc tcttgggagt agacggcttt
    26281 attattcagc cgacctctaa tttccgaaaa tattctcgta tcatcgatga gaaaaagaag
    26341 aaaatggtct tttttgatag tcagctctat gaacaccgga ctagctgggt taaaaccaat
    26401 aactatgatg ccgtttatga catgacccag tcctgtatcg aaaaaggtta tgagcatttt
    26461 ctcttgatta cagcggatac gagtcgtttg agtactcgga ttgagcgggc aagtggtttt
    26521 gtggatgctt taacagatgc taatatgcgt cacgccagtc taaccattga agataagcat
    26581 acgaatttgg aacaaattaa agaattttta caaaaagaaa tcgatcccga tgaaaaaact
    26641 ctggtattta tccctaactg ttgggcccta cctctagtct ttaccgttat caaagagttg
    26701 aattataact tgccgcaagt tgggttgatt ggttttgaca atacggagtg gacttgcttt
    26761 tcttctccaa gtgtttcgac gctggttcag ccctcctttg aggaaggaca acaggctaca
    26821 aagattttga ttgaccagat tgaaggccgc aatcaagaag aaaggcaaca agttttggat
    26881 tgtagtgtga attggaaaga gtcgactttc taaaataaag gaaaatgact tgcaatctct
    26941 attaagaaat aaaataatcc cacctagaac aagctaggtg ggattatttg cctatgaaat
    27001 gagaaattat gggagcaagc tcctaaatca actgtttttg atctacttct ttaactactt
    27061 gataaaagtt atagaagtag gccaaacttg aaatgatggt tacgactagg aatattgaaa
    27121 atttccattg gacagggttg gttaaaagtt gtggaaagga tatgaggaga aagaagaggg
    27181 ctgcgttgag gacaggtatc cgttttgatt gtattttctc aagtccttta ttgagcgcag
    27241 gaagaaagag gagtaggagt agtaaaactg tatgagaaac agctcctgaa ataagggcga
    27301 agaaaaggaa aatactgata aaaacatgaa tgatcagtag tctagctagt gatttcataa
    27361 ggtacctcct aatcctggtc ttttttagct cttgcaatac gaagtgagtc gacaatatgt
    27421 atcatcactc cgaaaaagaa agctcccagt atagttttaa aaatatgttt tgtatttaga
    27481 agagaactga taaaatttgg attttcactt gttagggtat caatgagtgg aattataaaa
    27541 aatatcactg ttccataaat cgaacctgct ttcagaccag gataacgtaa ctgtctcttt
    27601 tcttttttca tagggcacct cctaatcctc atcttgattt ttcttagttt ttgcaatgcg
    27661 acgggagatg aggaactgta tgctcgctcc gaagaaaata gaaccgagaa tacttgatac
    27721 accatttctt atagtgagaa gagaatgaaa atagtcctga ccttcatcta tgagtatact
    27781 gagaagagga gttataaaaa acatccatag accaaagaac aaacctgctt tcagacctgg
    27841 gtagtgtagt tgcttgcttt ctttctcatt cagcatatct ggttcaatga ctgtgatgcc
    27901 tgtttttttc atttggtagg tgacatagcc agcagctatg agggcaatca ctaaaatcag
    27961 aggaggatag attagagcca cttcttgagg gtatttatag gccagaagga gtggaataag
    28021 atttccgaaa atcatcagat aaaagaggac gataaaaact tggttcccaa tactatcggc
    28081 ctcacgccgt ttgtattcgt caaggggacc agaaataccg tatgtgcgtt tgatcagttt
    28141 ttcagtgaag gtttcttttt tcatgagttt gctccttttt taaaaatctt cctcccaaaa
    28201 gagactgttg aggtcagttt ggaggctgcg ggcgagattg agacagagtt ccaaggttgg
    28261 attgtacttg tcgttttcaa tcatattgat agtctgtctc gagacaccga tatccttggc
    28321 gagttcgagc tgggaaatac ccaattcctt gcgaaattct ttcacacgat tcatctgatc
    28381 tcctttttga tttatgtcgt atatatttga ctttattata gtcttttaaa cataaagtgt
    28441 caagtatttt tgacatattt tttgaagaaa tagtagtctc cttgtcctat ttgtctgaca
    28501 agtgcaagct ggtcggattt gtggtaaaat agataagata tgacaaaaga atttcatcat
    28561 gtaacggtct tgctccacga aacgattgat atgcttgacg taaaacctga cggtatctac
    28621 gttgatgcga ctttgggcgg agcaggacat agcgagtatt tattaagtaa attaagtgaa
    28681 aaaggccatc tctatgcctt tgaccaggat cagaatgcca ttgacaatgc gcaaaaacgc
    28741 ttggcacctt acattgagaa gggaatggtg acctttatca aggataactt ccgtcattta
    28801 caggcacgtt tgcgcgaagc tggtgttcag gaaattgatg gaatttgtta tgacttggga
    28861 gtgtctagtc ctcaattgga ccagcgtgag cgtggttttt cttataaaaa ggatgcgcca
    28921 ctggacatgc ggatgaatca ggatgctagt ctgacagcct atgaagtggt taatcattat
    28981 gactatcatg atttggttcg tattttcttc aaatacggtg aggataaatt ctctaaacag
    29041 attgcgcgta agattgagca agcgcgtgaa gtgaagccga ttgagacaac gactgagtta
    29101 gcagagatta tcaagttggt caaacctgcc aaggaactca agaagaaggg tcatcctgct
    29161 aagcagattt tccaggctat tcgaattgaa gtcaatgatg aactgggggc ggcagatgag
    29221 tccatccagc aggctatgga tatgttggct ctggatggta gaatttcagt gattaccttt
    29281 cattccttag aagaccgctt gaccaagcaa ttgttcaagg aagcttcaac agttgaagtt
    29341 ccaaaaggct tgcctttcat cccagatgat ctcaagccca agatggaatt ggtgtcccgt
    29401 aagccaatct tgccaagtgc ggaagagtta gaagccaata accgctcgca ctcagccaag
    29461 ttgcgcgtgg ccagaaaaat tcacaagtaa gagggaaaaa gatggcagaa aaaatggaaa
    29521 aaacaggtca aatattacag atgcaactta aacggttttc gcgtgtggaa aaagcttttt
    29581 acttttccat tgctgtaacc actcttattg tagccattag tattattttt atgcagacca
    29641 agctcttgca agtgcagaat gatttgacaa aaatcaatgc gcagatagag gaaaagaaga
    29701 ccgaattgga cgatgccaag caagaggtca atgaactatt acgtgcagaa cgtttgaaag
    29761 aaattgccaa ttcacacgat ttgcaattaa acaatgaaaa tattagaata gcggagtaag
    29821 atatgaagtg gacaaaaaga gtaatccgtt atgcgaccaa aaatcggaaa tcgccggctg
    29881 aaaacagacg cagagttgga aaaagtctga gtttattatc tgtctttgtt tttgccattt
    29941 ttttagtcaa ttttgcggtc attattggga caggcactcg ctttggaaca gatttagcga
    30001 aggaagctaa gaaggttcat caaaccaccc gtacagttcc tgccaaacgt gggactattt
    30061 atgaccgaaa tggagtcccg attgctgagg atgcaacctc ctataatgtc tatgcggtca
    30121 ttgatgagaa ctataagtca gcaacgggta agattcttta cgtagaaaaa acacaattta
    30181 acaaggttgc agaggtcttt cataagtatc tggacatgga agaatcctat gtaagagagc
    30241 aactctcgca acctaatctc aagcaagttt cctttggagc aaagggaaat gggattacct
    30301 atgccaatat gatgtctatc aaaaaagaat tggaagctgc agaggtcaag gggattgatt
    30361 ttacaaccag tcccaatcgt agttacccaa acggacaatt tgcttctagt tttatcggcc
    30421 tagctcagct ccatgaaaat gaagatggaa gcaagagctt gctgggaacc tctggaatgg
    30481 agagttcctt gaacagtatt cttgcaggga cagacggcat tattacctat gaaaaggatc
    30541 gtctgggtaa tattgtaccc ggaacagaac aagtttccca acgaacgatg gacggtaagg
    30601 atgtttatac aaccatttcc agccctctcc agtcctttat ggaaacccag atggatgctt
    30661 ttcaagagaa ggtaaaagga aagtacatga cagcgacttt ggtcagtgct aaaacagggg
    30721 aaattctggc aacaacgcaa cgaccgacct ttgatgcaga tacaaaagaa ggcattacag
    30781 aggactttgt ttggcgtgat atcctttacc aaagtaacta tgagccaggt tccactatga
    30841 aagtgatgat gttggctgct gctattgata ataatacctt tccaggagga gaagtcttta
    30901 atagtagtga gttaaaaatt gcagatgcca cgattcgaga ttgggacgtt aatgaaggat
    30961 tgactggtgg cagaatgatg actttttctc aaggttttgc acactcaagt aacgttggga
    31021 tgaccctcct tgagcaaaag atgggagatg ctacctggct tgattatctt aatcgtttta
    31081 aatttggtgt tccgacccgt ttcggtttga cggatgagta tgctggtcag cttcctgcgg
    31141 ataatattgt caacattgcg caaagctcat ttggacaagg gatttcagtg acccagacgc
    31201 aaatgattcg tgcctttaca gctattgcta atgacggtgt catgctggag cctaaattta
    31261 ttagtgccat ttatgatcca aatgatcaaa ctgctcggaa atctcaaaaa gaaattgtgg
    31321 gaaatcctgt ttctaaagat gcagctagtc taactcggac taacatggtt ttggtaggga
    31381 cggatccggt ttatggaacc atgtataacc acagcacagg caagccaact gtaactgttc
    31441 ctgggcaaaa tgtagccctc aagtctggta cggctcagat tgctgacgag aaaaatggtg
    31501 gttatctagt cggggtaacc gactatattt tctcggctgt atcgatgagt ccggctgaaa
    31561 atcctgattt tatcttgtat gtgacggtcc aacaacctga acattattca ggtattcagt
    31621 tgggagaatt tgccaatcct atcttggagc gggcttcagc tatgaaagac tctctcaatc
    31681 ttcaaacaac agctaaagct ttggagcaag taagtcaaca aagtccttat cctatgccta
    31741 gtgtcaagga tatttcacct ggtgatttag cagaagaatt gcgtcgcaat cttgtacaac
    31801 ccatcgttgt gggaacagga acgaagatta aaaacagttc tgctgaagaa gggaagaatc
    31861 ttgccccgaa ccagcaagtc cttatcttat ctgataaagt agaggaagtt ccagatatgt
    31921 atggttggac aaaggagact gctgagaccc ttgctaagtg gctcaatata gaacttgaat
    31981 ttcaaggttc gggctctact gtgcagaagc aagatgttcg tgctaacaca gctatcaagg
    32041 acattaaaaa aattacatta actttaggag actaatatgt ttatttccct cagtgctgga
    32101 attgtgacat ttttactaac tttagtagga attccggcct ttatccaatt ttatagaaag
    32161 gcgcaaatta caggccagca gatgcatgag gatgtcaaac agcatcaggc aaaagctggg
    32221 actcctacaa tgggaggttt ggttttcttg attacttctg ttttggttgc tttctttttc
    32281 gccctattta gtagccaatt cagcaataat gtgggaatga ttttgttcat cttggtcttg
    32341 tatggcttgg tcggattttt agatgacttt ctcaaggtct ttcgtaaaat caatgagggg
    32401 cttaatccta agcaaaaatt agctcttcag cttctaggtg gagttatctt ctatcttttc
    32461 tatgagcgcg gtggcgatat gctttctgtc tttggttatc aagtgcatct agggattttc
    32521 tatattgtct tcgctctttt ctggctagtc ggtttttcaa acgcagtaaa cttgacagac
    32581 ggtgttgacg gtttagctag tatttccgtt gtgattagtt tgtctgccta tggagttatt
    32641 gcctatgtgc aaggtcagat ggatattctt ctagtgattc ttgccatgat tggtggtttg
    32701 ctcggtttct tcatctttaa ccataagcct gccaaggtct ttatgggtga tgtgggaagt
    32761 ttggccctag gtgggatgct ggcagctatc tctatggctc tccaccaaga atggactctc
    32821 ttgattatcg gaattgtata tgtttttgaa acaacttctg ttatgatgca agtcagctat
    32881 ttcaaactga caggtggtaa acgtattttc cgtatgacgc ctgtacatca ccattttgag
    32941 cttgggggat tgtctggtaa aggaaatcct tggagcgagt ggaaggttga cttcttcttt
    33001 tggggagtgg gacttctagc aagtctcctg accctagcaa ttttatattt gatgtaagaa
    33061 tggcaccctg atgtttcagg gtgtttttgt gtttaaatac acaatgaaaa tcaaagaaca
    33121 aactagaaag ctaactttag gctgctcaaa atatagtaga ttgaaactag aatagtacac
    33181 atctacttct aaaatattgt tagaaatcga tttgactgtc ctgattgatt tgtcctgttc
    33241 ttatttcatt ttaatataca gttttgaggt tgtagataag gagaagctga tgtggtttga
    33301 agaggttttc gaagagtgta aaaaaatatt ggtcagttaa agtcaaaact cttgaccttt
    33361 tctgaccaat gtagtatatt atgaacgtaa ggtaaatgaa agccttacta gaacacttat
    33421 ttaaagtaaa atagaaagag gtgggtctat gtttcatcta gaaatcttca gaagtaaaga
    33481 tagtctactc ctgcttgaaa aagaaaaacc agaaatagta cgtagggtag cgatctagct
    33541 agtctaaatt ttttaaaaca atggtcaaag atagtcaata tcagtaatca taactaagta
    33601 aacaaaaaga ggtaaagaat atgaataaca actttaataa ttttaataac atggatgatt
    33661 tatttaacca attgatgggt ggtatgcgag gatacagttc tgaaaatcgc cgttacttaa
    33721 ttaatggacg cgaagtcaca cctgaggaat ttgctcacta tcgtgcgact ggtcaattac
    33781 caggaaatgc agaaactgat gtgcaaatgc cacaacaggc atcaggtatg aaacaagacg
    33841 gtgtccttgc aaaactaggt cgaaacttaa cagcagaagc gcgtgagggc aagttggacc
    33901 ctgttatcgg acgaaacaag gaaattcaag aaacatctga aatcctctca cgccgcacca
    33961 agaacaatcc tgttttggtc ggagatgcag gtgttggtaa gacagcagtt gtcgaaggtc
    34021 tagcgcaagc cattgtgaac ggagatgttc ctgctgctat caagaacaag gaaattattt
    34081 ctattgatat ctcaggtctt gaggctggta ctcaataccg tggtagcttt gaagaaaatg
    34141 tccaaaactt agtcaatgaa gtgaaagaag cagggaatat tatcctcttc tttgatgaaa
    34201 ttcaccaaat tcttggtgct ggtagcactg gtggagacag tggttctaaa ggacttgcgg
    34261 atattctcaa gccagctctc tctcgtggag aattgacagt gattggggca acaactcaag
    34321 acgaataccg taacaccatc ttgaagaatg ctgctcttgc tcgtcgtttc aacgaagtga
    34381 aggtcaatgc tccttcagca gagaatactt ttaaaattct tcaaggcatt cgtgacctct
    34441 atcaacaaca ccacaatgtc atcttgccag acgaagtctt gaaagcagcg gtggattatt
    34501 ctgttcaata cattcctcaa cgtagcttgc cagataaggc tattgacctt gtcgatgtaa
    34561 cggctgctca cttggcggct caacatccag taacagatgt gcatgctgtt gaacgagaaa
    34621 tcgaaacgga aaaagacaag caagaaaaag cagttgaagc agaagatttt gaagcagctc
    34681 taaactataa aacacgcatt gcagaattgg aaaagaaaat cgaaaaccac acagaagata
    34741 tgaaagtgac tgcaagtgtc aatgatgtgg ctgaatctgt ggaacgaatg acaggtatcc
    34801 cagtatcgca aatgggagct tcagatatcg aacgtttgaa agatatggct catcgcttgc
    34861 aagaaaaggt gattggtcaa gataaggccg tagaagttgt agctcgtgct atccgtcgta
    34921 accgtgctgg ttttgatgaa ggaaatcgcc caatcggcag cttcctcttt gtagggtcta
    34981 ctggggttgg taagacggag cttgctaagc aattggcact cgatatgttt ggaacccagg
    35041 atgcgattat ccgtttggat atgtctgaat acagtgaccg cacagctgtt tctaagctaa
    35101 ttggtacaac agcaggctat gtgggttatg atgacaatag caatacctta acagaacgtg
    35161 ttcgtcgcaa tccatactct atcattctct tggatgaaat tgaaaaggct gatcctcaag
    35221 ttattaccct tctcctccaa gttctagatg atggtcgttt gacagatggt caaggaaata
    35281 cagtaaactt caagaacact gtcattattg cgacctcaaa tgctggattt ggctatgaag
    35341 ccaacttgac agaagatgcg gataaaccag aattgatgga ccgtttgaaa cccttcttcc
    35401 gtccagaatt cctcaaccgc tttaatgcag tcatcgagtt ttcacacttg actaaggaag
    35461 acctttctaa gattgtagat ttgatgttgg ctgaagttaa ccaaaccttg gctaagaaag
    35521 acattgactt ggtagtcagt caagcggcta aagattatat cacagaagaa ggttacgacg
    35581 aagtcatggg ggttcgtcct ctccgtcgcg tggttgaaca agaaattcgt gataaggtga
    35641 cagacttcca cttggatcat ttagatgcta aacatctgga agcagatatg gaagatggtg
    35701 gtttggtcat tcgtgaaaaa tcctaatact cttcgaaaat caaattcaaa ccacgtcaac
    35761 gtcgccttgc cgtactcaag tacagcctgc ggctagtttc ctagtttgct ctttgatttt
    35821 cattgagtat aagatagaat tttgaggata aaaaagaagg agccagctga aaaaaactgg
    35881 ttccttttga ggtacgacgg gcatgtcgtg cagtagag
//
LOCUS       DAVDWT010000025        28300 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVDWT010000025 DAVDWT010000000
VERSION     DAVDWT010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28300
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.025"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            486..605
                     /locus_tag="VUW50_001391"
     CDS             486..605
                     /locus_tag="VUW50_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET7983275.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            complement(701..2047)
                     /gene="glnA"
                     /locus_tag="VUW50_001392"
     CDS             complement(701..2047)
                     /gene="glnA"
                     /locus_tag="VUW50_001392"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET7983276.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(2084..2440)
                     /gene="glnR"
                     /locus_tag="VUW50_001393"
     CDS             complement(2084..2440)
                     /gene="glnR"
                     /locus_tag="VUW50_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HET7983277.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            complement(2517..3044)
                     /locus_tag="VUW50_001394"
     CDS             complement(2517..3044)
                     /locus_tag="VUW50_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET7983278.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            complement(3180..4376)
                     /locus_tag="VUW50_001395"
     CDS             complement(3180..4376)
                     /locus_tag="VUW50_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET7983279.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            complement(4466..9445)
                     /locus_tag="VUW50_001396"
     CDS             complement(4466..9445)
                     /locus_tag="VUW50_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HET7983280.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLHYQGHEVSFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASSEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELTTAKQSLKDLVALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELTFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNKVLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            9738..11369
                     /locus_tag="VUW50_001397"
     CDS             9738..11369
                     /locus_tag="VUW50_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HET7983281.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHETINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            complement(11928..13535)
                     /locus_tag="VUW50_001398"
     CDS             complement(11928..13535)
                     /locus_tag="VUW50_001398"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET7983282.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAAAMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDAEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(13925..14512)
                     /locus_tag="VUW50_001399"
     CDS             complement(13925..14512)
                     /locus_tag="VUW50_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HET7983283.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(14640..15089)
                     /locus_tag="VUW50_001400"
     CDS             complement(14640..15089)
                     /locus_tag="VUW50_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7983284.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(15094..15483)
                     /locus_tag="VUW50_001401"
     CDS             complement(15094..15483)
                     /locus_tag="VUW50_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HET7983285.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(15511..16137)
                     /locus_tag="VUW50_001402"
     CDS             complement(15511..16137)
                     /locus_tag="VUW50_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7983286.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKNPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(16151..16714)
                     /locus_tag="VUW50_001403"
     CDS             complement(16151..16714)
                     /locus_tag="VUW50_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET7983287.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(16879..17113)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(17167..17670)
                     /locus_tag="VUW50_001404"
     CDS             complement(17167..17670)
                     /locus_tag="VUW50_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET7983288.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(17863..18693)
                     /locus_tag="VUW50_001405"
     CDS             complement(17863..18693)
                     /locus_tag="VUW50_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET7983289.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(18678..20360)
                     /locus_tag="VUW50_001406"
     CDS             complement(18678..20360)
                     /locus_tag="VUW50_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7983290.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQIQLQTELVKETPALQSLFILEEVSFSYNDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVNEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(20462..20719)
                     /locus_tag="VUW50_001407"
     CDS             complement(20462..20719)
                     /locus_tag="VUW50_001407"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983291.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(20726..21274)
                     /locus_tag="VUW50_001408"
     CDS             complement(20726..21274)
                     /locus_tag="VUW50_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HET7983292.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(21289..22137)
                     /locus_tag="VUW50_001409"
     CDS             complement(21289..22137)
                     /locus_tag="VUW50_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HET7983293.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            22325..23674
                     /gene="trkA"
                     /locus_tag="VUW50_001410"
     CDS             22325..23674
                     /gene="trkA"
                     /locus_tag="VUW50_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HET7983294.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            23678..25117
                     /locus_tag="VUW50_001411"
     CDS             23678..25117
                     /locus_tag="VUW50_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET7983295.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(25244..25468)
                     /locus_tag="VUW50_001412"
     CDS             complement(25244..25468)
                     /locus_tag="VUW50_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HET7983296.1"
                     /translation="MIANSGAYGAHYDIMGVYDLIILAPQVRSYYREMKVDAERLGIQ
                     IVATRGMEYIHLTKNPSKSLQFVLEHYQAV"
     gene            complement(25480..25701)
                     /locus_tag="VUW50_001413"
     CDS             complement(25480..25701)
                     /locus_tag="VUW50_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IIBC"
                     /protein_id="HET7983297.1"
                     /translation="MEVVLFSYCLNTRYLLQVIDIASSNDIILEEDTSEIMPGELDEI
                     KSKELKVLVLCAGSGTSAQLANAINKGLT"
     gene            26262..26432
                     /locus_tag="VUW50_001414"
     CDS             26262..26432
                     /locus_tag="VUW50_001414"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7983298.1"
                     /translation="MTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEEIG"
     gene            27044..27328
                     /locus_tag="VUW50_001415"
     CDS             27044..27328
                     /locus_tag="VUW50_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983299.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            27321..27878
                     /locus_tag="VUW50_001416"
     CDS             27321..27878
                     /locus_tag="VUW50_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter peripheral membrane
                     component"
                     /protein_id="HET7983300.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVGGYVLEYHGLRATQDVD
                     AFYDQNQKINEIIARVGKQFNLNTHEELWLNNHVANMNKQPPLSLCESLYSFENLTVL
                     VVPIEYVLGMKMMSIREQDLKDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVLLEG
                     FSYAYGMDWLEKFFKENQDKLREFY"
BASE COUNT         8325 a         6160 c         5132 g         8683 t
ORIGIN      
        1 tatgtgcttc aagtatataa aaaggcgtaa aaatacgcct atagataatg gggttgaaat
       61 aggtttattg ttgatgagat tgtagataat tcaatttttt acttccaatc gaatattcaa
      121 agcctccacc ttttctgcct gtaattgttc atcataaaat tcaatatctt caggattttc
      181 cccttgacaa cctcggcaga aatattcttc cgctcgatca ggattcaaaa atcgacaagc
      241 acaaacaaaa cagtcgccat catcatttat tgagataata tagtagattg aaataagatg
      301 taaacaaatc gattaggaaa gttaaattaa tttctagaaa tttttagcag atgtagtgta
      361 ctattctagt ctcaatttac tatggcttca aatatatctt tcgaaaaaat atttacagat
      421 gtgtaatttt gaagcttgca aaagttagta aacttgtaga tttcgatttg aagtaacttg
      481 ttttcttgcc cgatattgtt tttgaaattg aatttttcca tagtgactcc ttgattttct
      541 tctacacgtc tgatgataaa tctaattcgc aaaagagtca agaggatttt tcgaaaaata
      601 aatagcgacc gaaatcgcta ttttaagggt tataggtatt tgatggctta gactgctgtg
      661 tgactgttta cccacaggca atctttcttc tatattagta ttagtaaagg tctaaataat
      721 tatcaatttc ccattgtgaa acgaaggttg cataacttgc ccattcgatt cgtttggctt
      781 caaggaagct agtgtagatg tgatctccga gagcagcttt aaccacttca tcttctgtca
      841 aagctttcaa agcgttgtga agagttgatg gaaggtctgt aataccagct tccttgcgct
      901 cttctgctgt catgatgtag atattttctt cgataggagc tggtgcttcg attttatttt
      961 caataccata caaaccaact tccaaaagaa cagccatagc aatgtaaggg ttcgccattg
     1021 gatccactga acgcaactca agacgagttc ccataccacg tgaagcaggt acgcgcacaa
     1081 gtggcgaacg gttacgacca gcccaagcaa tgtaaacagg cgcttcataa cctggaacca
     1141 aacgtttgta tgagttaact gttgggttca tgatggcagt atagttgtaa gcatgcttga
     1201 tcaaaccgcc taggaaatgg taagctgttt ctgacaactg cattcctttt ggatcatttg
     1261 gatcaaagaa ggcgttattt ccttctgcat caaacaagga catattacag tgcatacctg
     1321 atccagcaat accaaatttt ggcttcgcca taaatgttgc gtaaagtccg tgtttgcgag
     1381 caatggtttt aacaacaagc ttaaagattt gaatcttatc acaagcacgg agaacttcat
     1441 cgtacttaaa gtcaatctca tgctgtccaa ccgcaacctc gtggtgactc gcttctactt
     1501 caaatcccat tttggtcaag acattcacaa tctcacgacg tgtgttgtcc gcaaggtcag
     1561 taggtgccaa atcaaagtag ccacccttgt cattcacttc aagtgttggg tccccatttt
     1621 catccaactt aaataggaag aattctggct ctggaccaag gttgaaggat ttgaatccaa
     1681 cttcttccat gtgacgaaga gctcgtttca aattaccacg agggtcaccc gcaaatggtt
     1741 caccttctgt tgtatagaca tcacagatca gacctgcaac acttccattt tcatctcccc
     1801 aagggaagac tgtccatgta tccaagtccg ggtacaagta catatccgac tcattgatac
     1861 gtacaaaacc ttcaatagaa gatccatcaa acataacctt gttcgacaag accttatcta
     1921 actgttcatc tgtagcagga atttcgacgt ttttcatggt tcccaaaata tctgagaaca
     1981 taagacgaat aaaggtaaca tttttttcct tgacttcacg acgaatatct gcagctgtga
     2041 ttggcataag ttttctcctt aatctatgac tacttgcggt tgcctaaccg cgaccaaaag
     2101 gtgactgtac tgaagcaaaa cgcccctgtt ggaggagttc attgtgaagt gcacgacgta
     2161 cttcagtctg actaaccgct ttcttggatt tcgcttcacg ttcagcatat tttttcttaa
     2221 tggcagcgat attataacct tcagagatat aatctttgat ttcaagcaga cgatccatgt
     2281 cattcaagga atacatgcga cgatttcctt cgtttcgatc gggcttgatc aactcttgat
     2341 cttcataata acgaatctga cgcgccgata gatcggtcaa cttcataaca ctgccgatag
     2401 gaaaaacagc catatttcgg cgaaattctt tttccttcat ttacaatttc cttctttctg
     2461 tctattatag tctaaaaaaa gacaaacgtc aattgataat gttataaaat gtaacattat
     2521 ttttcttttt tctctaaaaa gagacgaata cgatcaatat cgtaatttac gataattgcg
     2581 acaaaaactc ccataaacgt ttctaataca cgcacaaaca cgtacaaaat tgtctcgcca
     2641 cttggaattg atagggtaat gattaacata gctgctacac caccaataac ccctgctttg
     2701 ttattcatgg ctacatttgt cataatggtt aacatggtgc agattggaac aactaccaag
     2761 gtcacccaaa aggcttcgtg gaaaaaagta tttaataaga agaagaccaa ggcatagagt
     2821 ccaccgatac tatttcctag aatacgcgaa gtcccaaaat gaacactctc atcaaaactc
     2881 tccctcaggc taaaaacggc tgtcaaagca ccaatttgaa gacctttcca gccaaaaaag
     2941 ccaaaaatca agagaactag aaaaacagca atacctgttt taaaggttcg cataccaagt
     3001 ttgaactggg atttatcgaa tttatatttt ttaaaataac tcataatctc aactttctat
     3061 ttccatttta tcataaatcg gtgattttta tgagtaatag ttgagaggaa gcgtttttat
     3121 tttaagcaaa agaaaagagg aactttcatc cctctcttct ttgatttgtt tataaaatct
     3181 tatttttctg tcaaggctgc aagtcctgga agaaccttac cttcaagaag ttccattgat
     3241 gctccaccac ccgtactaat ccatgagaac ttgtctgcac ggccaaggtt aatcgctgcg
     3301 gcagctgagt caccaccacc gatgattgat ttaactcctg gttgtttcac gatagcgtcc
     3361 atcacaccga ttgtaccagc ttggaaatct gggttttcaa atacacccat aggtccgttc
     3421 catacaactg ttttggcacc agtcaaagct tcgtcaaatt tggcgataga ttttggaccg
     3481 atgtcaagac caaggaagcc ttcagaaact gcttcacctt cagtgtcacg cacttcagtg
     3541 taaccagcaa atgcgttagc ttcttttgag tcaactggca agatcaattt accatttgct
     3601 ttttcaagaa gagctttcgc aacatccaat ttgtcttctt ctacaagtga gttaccgatt
     3661 tcgatacctt gtgctttgta gaatgtgtaa gtcatcccac caccgataag gactttatca
     3721 gctttttcaa gcaagttttc gataacaccg atcttgtctg aaacttttga accaccaagg
     3781 atagccacga atggacgttc tggagtttca actgcttctt ggatgtaggc aatttcgttt
     3841 tcaagaagga aaccagcaac tgctttttca acgtttgctg agataccaac gttagatgcg
     3901 tgtgcacggt gagctgtacc gaatgcatcg tttacgaaga taccatctcc aagtgatgcc
     3961 cagtatttac caagttcagg atcgttttta gattctttct tgccgtcaac atcttcgtaa
     4021 cgagtgtttt caaccaagag aacttgtcca tcttcaagag cgttgattgc cgcttccaat
     4081 tcagcaccac gagtgacacc tgggaaaaca acatcttgac caagttttgc tgccaagtca
     4141 gctgctacag gagcaagtga tttaccagct ttatcagctt cttctttcac acgtccaagg
     4201 tgagagaaaa gaattgcacg tccaccttgt tcgatgatgt acttaatagt tggaagagct
     4261 gctgtgatac ggttatcgtt agtgattacg ccatctttca atggtacgtt gaagtcaaca
     4321 cgaacgagga cttttttacc tttcaagtca acgtctttaa cagtaagttt tgccatgtta
     4381 caaaaactcc tttatttatt ttattcgaaa ctattatatc acactttgga aatataagga
     4441 aagatttcac aagatttttc gatatttaat ctttatcttc tcttttccgt ctcaaggtca
     4501 aggctaaact agcaagaccg aggctggcta agcctgctat tagggcttca ttagcatcag
     4561 ctgttcctgt atttggcaat tgtttgcttg cttcttctga tttaactgct gtattttgag
     4621 ctggtttttc ctgtgtagct actactgctg gtactgtttt tacttttgtt ccaatttcaa
     4681 caatttctgg aatcgcttct tggatgactt ctgttgaacg aagacgacgc tgaccgtttt
     4741 catcaacttc aaagacatgt cttaacagtc catctcgtcc ctgtatgatg actcgttgtt
     4801 ccccaactag catctcagca ttttcatgct cttgacgatg aaaggcaacc ttttcctctt
     4861 gaacttccaa tttaggagcc ttttgttgat cagaagccgg ttgataagta ggttttggaa
     4921 ggactttgtt ttgtggtgct ggtttagcag gttgtggttt ttcagcagat tcataaacaa
     4981 aggcataatg agtaaagtgt ggtgctgtaa agataacatg actatccgtt tgttcaaaag
     5041 tcaattctac tgcctctttg ccttcaggta agaaaaatac tttcttaact ttcttatctt
     5101 tttcaattgg gattttcaca atagaagcgt aagagagatc aacatcttga cctttttcat
     5161 ccaaaccttc tatttcaaag acatgagcat cttctccagc aaagtatttc ttctcttcag
     5221 cactcgcttg aacacgctct actttcaaac ccttgatgac agtcttctct ttatttgaga
     5281 agtgtacttc aacacctgtt ttactatcag aaaagactgc tggcttgtct tctttcaata
     5341 aagcaaccag atcttttaga ctctgtttcg ctgttgtaag ctcttctgga ctcaaatcta
     5401 ctttttcaac caaggccttg tttttctcaa gaagcggagc tagggcagaa cctgaaggat
     5461 ggtttggttt ggcagccaag agttcttgaa cctcacctgt taagcgatca agctctgtct
     5521 tttcttccgt aaatttctct tgaccattca gagctttatg agcttctgtc aatttattaa
     5581 gtcggtcatt gacttgatct tgggtgctag tttcatggtc tttcaacact tgttcggccg
     5641 cttcaattgc tttcaagaag gcttctttta cttctgatga agcaaagtta tagaccacct
     5701 tgttcttagc agcttcaact tctgctaatt tttgtttgag gtactcatcg ttcaatgctg
     5761 cttttggaga taccaaaaca tcaaagctaa cttcatggcc ttggtaatga agcgtcaagg
     5821 tttgacgacc agtcttttga gcatcgtaac cagaaatttc aactccctca tcagtaaagg
     5881 aatgttcttc catggtgtca ttgctataag ccactgcaaa gcgtccttca gacaagtcta
     5941 agctatcacc aactaggtaa tcttttttcg gttcctgact tacttcaatt cccaaaatag
     6001 ttttcggact tgcttcgtct tggccagtga cagtcactga caaattagca tttaccggtt
     6061 gtcccaaata ttggagagta agattctgtt ctcccttatg atgcgtatca aaacctgata
     6121 ctgatacacc tgcgtgagtt aggcgaatga gttcgtcctc agttcctcct tcatactgaa
     6181 ctcgaagaac accacctcta aggtccaaat cctccccttc ggcgtagctt gttttcttag
     6241 gtcctgtttc taggctgact gacttgattc ttctgtcatc ttttggaacg gaaactgcta
     6301 ggacattact aatttccttg cctggcaact gggtacgata ataaagaaga ccagattgat
     6361 ttgtcaaatc caatggtgca cttgctaggt ctcctccaac ttcgctcttc aaggttgcga
     6421 gcggagtttg agaatttgga ttatcataaa cggtaatagt tgctccagcc ggtacatctg
     6481 caaagccaac ttgtaccttg ttattgccta gagaacgggc tgcagccttg gccatcggaa
     6541 tattgacact ctcagtatca agcttttcat acattttcca gttatagata cgaatagcct
     6601 tccatggagt tccattgtca gaagtgacaa cattcaagcg ccagtcttga gcagtgattg
     6661 gtttatcaag agtgatatct gtcacgtgtg ctttgttacc acggacttcc ttagctagct
     6721 tccactcacc atctgcatct ttataataaa ggtcaaagtc tttagtgttc atcaagccat
     6781 cgttaacaga ctcaccacca gctcctgcat gatccatgac ccatctaaca acggtacgtg
     6841 gcttggtcaa acgaatatcc acactaccac tcaactgagc tgaagaccat ttatctgaca
     6901 agctagtaat ggtaccgttc aacatacctt caataccttc tccaccttca gtcttaggga
     6961 aagtactatc aataactgtt gcacctggaa cgatattttc agctagtggt tttggtaggc
     7021 tggtatcttt tacagtcata ccccaatcaa aggttgtggt tgcagcttct gaacgaactc
     7081 catttttacc gactgctaca accttcagtt cttgagttgt accctgagca cttgctgagc
     7141 ggctaacttt tggtagataa atagttgtag aagatgagcc agttagtaat ttccagctgt
     7201 ctccatcttt ttcataaact tcatagaaat ctgcatcctt gttgccttta aattgcacaa
     7261 ctgcttccgc ttcttgggca tttttaagag attgtttcac tacagaaaag cttgtcggcg
     7321 attgtggctc ttggtgattg tccgagatag ttaattgtcc taggttaaac tgataatctt
     7381 ttacagcacc ttcatgctcg aaaaatagtt tgactgcata gatggtttta cccgctagtg
     7441 agctaagatc aaattcttca tttgtccagt tgtcagaaag ggttagctct ttccatgcat
     7501 ctgcatcatc gaatttgtag tctggagttg tagagaatgc catataaact ttagaacctt
     7561 ttcctccctt gtgggcaaca cgaagtttgg tcttctcagt tacttctaac ttagtagaat
     7621 aaagtctcac atcctgatct gtcttaccgg ctacatcacc agagaattta agggaatttc
     7681 cgccattata ggcatctgta aaatcatatt ctgcacgaag tttttcccct gttgaagtct
     7741 gccaccagcg ccatgttgga agaacacctg atactgaacg ataattccac tcagaatcct
     7801 tagaaacctt accatctacg aaccattttt taccatgacc tgtattaaaa gaagtagtaa
     7861 aagtattacc taccgctggc gtacggtccg caactaggtt agcaatacca taccaatctt
     7921 tgtcacctgg tttttggcca gtagggtctc cttgataacc tgtaaagaag atatcttcat
     7981 ttttatgata atcttcacca gtttttccta aacttgtaat ggtatctggg gcaaataaac
     8041 caagagaaag gcgcaatttc ccattttcgt ctaaaatgtc attccactta acctttgtct
     8101 tgtaggaacc accctgttgc aattccaaac ctgcaaatac atcataagga ttacgaccaa
     8161 tccagttggc agttgcaata gtgtaatcat ttttagcctt atcccagtta aagttagcaa
     8221 agaagttatc tgccggaacc ttatctcctt ctggttgcat gaattggtag ttgtattctc
     8281 ccaaaccatc ttgatgataa cgtccatagt tataggtcat ggcatcgtac caagaatact
     8341 tgattggatg gtttacctta gcagcatatt ccttgctata gagcataaac tggcgcatct
     8401 tttctccaag aggtttaacc aaatctccag ttgtttcttg gttgatgaaa tagccatcat
     8461 agccataata cttggccatg tctaccaatt tacgggcaat tgggaagcta ccatctgcgt
     8521 cttgcttcaa agcttcagca aatctttctt gatctgcaat actattagac cagttgaaga
     8581 agagtgtacc gtatacagga accccgttac ggtgacctgc atcaataacg tcaggagttg
     8641 gtacgagacc ttcccagaag accattgaat ctagatattg ccaatagtca aaagcatagg
     8701 ccttgaactc ttctccacca acagaagcat ggtcttttgc tttagaattg gtgtttgata
     8761 aggcttgaac ttttgcttcc ttgctagctt tttcattgac taaatgacct gtccgacgtg
     8821 aagcgaggac aacagatccg cggttaatag catcatcttc acgagcacct ggttcccatt
     8881 ttaacaattc ttcccaagaa ttaaatttga tttcctttgg ttttaatgtt ttttctgtat
     8941 ttttagggac atctggctgg acttcttttt cagtcttatt agcttctgct gctggtttag
     9001 actcatcccc ttggttagtt gcttcgggct ttgcttctgt ttccttgtca gacttaggtt
     9061 ttgcttctgc agatggtgtt tctgcaacca cctcacttgc tttttcctct ggtttagctg
     9121 cgctagctgc ttctgcagtt tcagtagttg cagcttcatt gcttgctggc gaagcagttt
     9181 cagatgctat cgctactcct tctgcctttt ctagcttgtt ttctctagca tgttcccctt
     9241 cattctgctt atcagcttca ttggtcgaat gctcactctc tcctgaaaca agctctgtat
     9301 tagctccgct attttcagga actgcagttt cttcagccaa ggctggacca gcaaataaaa
     9361 cagcaccaat catcagcgag caggctccta ctgataactt acgaatactg taacgacaac
     9421 gtctttcaaa aaatggattc ttcattttct cctcctaaaa gattgatttt gttgttttca
     9481 agaaagcgct ttatattctg tggtttttta tcaaactcta gaaccatgat aatcacattt
     9541 ttatcctaaa acatttttag acaaaaaaca ataactgttt gcacaaatct aaaataaggt
     9601 taaaaactct taaaaataat acacttatca attataattt tcttttacaa acttgacatt
     9661 aacacttgtt agatttactt ttatccttta aaatagaata ggagatattc cgcgattatt
     9721 tttcggagga aaaagaaatg tccattaatt ggcaggaaat tttatttcac tttttaggtg
     9781 gtctggggct attcttatat agcatcaaga ccatgggaga cggtttgcaa caagctgctg
     9841 gagatcgcct tcgtttttac attgacaaat atactagtaa tcctttcttt ggagttctgg
     9901 ttggtattgg gatgactgct ctaattcagt ctagttctgg tgtaacagtt atcacagtcg
     9961 gcctggtcag tgccggtctc ttaaccttac gtcaggctat cgggattgtc atgggtgcta
    10021 atattgggac aactgtcaca tcctttctca tcggttttaa attaggtaac tatgccctac
    10081 ctatgctctt tatcggtgcc gtctgtctct tttttacaaa aaatcggaca gtcaataata
    10141 tcggacgcat cctctttggt gtcggtggta tcttttttgc cctcaatctc atgagcggcg
    10201 caatggctcc actcaaggat ttacaggtct ttaaggacta tatgattgag ctaagtaaga
    10261 atcctgtttt gggtgtcttt gtcggtaccg gcttgacctt gctaattcaa gcttcttcgg
    10321 ctaccattgg gattttacaa aacctctacg ccggcaatct aattgaccta cagggagctt
    10381 tgccagttct atttggtgac aatatcggga caaccattac agccatcatt gcctctttag
    10441 gggctaatat tgcagctaaa cgggtagcag gagctcatgt tgccttcaac gttatcggaa
    10501 cagtcgtctg cgttattttt ctagttcctt ttactgtcct gattcattgg tttgaagcta
    10561 cgctaaatct agcaccggaa atgaccatcg cctttgctca cggaaccttt aatattacca
    10621 acaccattgt ccaatttcca tttatcggag ctctggctta ctttgtaacc aagattattc
    10681 ctggagagga cgaggttgtc aaatacgaac ccttatatct tgatgaacat ttcatcaaac
    10741 aggccccatc tatcgctcta ggaaatgcta agaaagagct cttgcactta ggaaactacg
    10801 ctgctaaagc ctttgacctt tcctataagt acatcattga cttggatgaa aaagttgctg
    10861 aaaaagggca taaaaccgaa gaagcaatta acaccatcga tgagcaatta acacgttatc
    10921 tcattgccct ttcaagcgaa gctctcagcc aaaaagaaag tgaagtgctt accaatatcc
    10981 ttgattcctc ccgtgatttg gaacggattg gagaccacac ggaggctcta ctcaatctga
    11041 ctgactatct tcaacggaaa aatgttgaat tttctgatgc cgccttgaaa gaattagagg
    11101 aagtttaccg ccaaactagt gactttatca aagatgctct ggatagtgtg gaaaacaatg
    11161 atattgaaaa agcacgcagt cttgtagaac gtcatgaaac aatcaataag atagaacgtg
    11221 ttctcagaaa aacccacatc aaacgcctca acaaaggcga atgttcaaca caagctgggg
    11281 tcaactttat cgacatcatc tcacactaca ctcgtgtatc agaccacgct atgaaccttg
    11341 ctgaaaaggt ttttgcagaa caaatctaag aaccaagaag ctatccatca taattggatg
    11401 gctttttact ttttcctaag caagactagg ctgaatgaaa ctgaaagagt attctgcaga
    11461 tatatagtat attgaaacta gaatagtaca cctctatttc taaaacattg ttagaaatcg
    11521 atttgactgt cctgatcgat ttgtcctgtt cttatttcat tttactatag tggattaaat
    11581 taaaaaagta taatgtattg aatctataac agtacatctt gactgctaaa atatttctat
    11641 aaattaattt gactttcctg atagagatgt tcacatctta tttcaattca ctatacaatc
    11701 cagttcctaa aactttgcta gaaattgatt tggctttccg aattaatttg ttcaaatttt
    11761 atttcatttc aatatactga taaatccatt cactagcaca gggcaagagg gagtcgctat
    11821 tgatttcaac ggtagcaggt acatacagag gacccctacc tcagatatac tgcaatatca
    11881 tcgtatgcaa aaaacctctg agatttttct caaaggttct gattttgcta attgctgttc
    11941 tcaaccgctg cagtaacaaa ggcagtgtag agttcttctg gtcggtttgg acggcttgac
    12001 agttcagggt gatactgaca agctacaaag aatttatttt caggaatttc cacgatttct
    12061 accaaacgat tgtctggaga aactcctgaa aagacaaaac ctgctgcctc aaactgctca
    12121 cggaaggcat tattaaactc ataacggtga cggtgacggc gttgcaccac ttcttgattg
    12181 tgataagcag cagcagcctt agagccacgt ttcaacttag acggataaag tcccaaacga
    12241 agggttccac ccatatcctc aatatcaatc tgatcacgca tgatatcaat gatagggtat
    12301 tttgtttctg gtgcaagctc tgcagaattg gcaccttcaa gacctaaaac gtgacgagca
    12361 aactcgatac atgtcaactg cattcccaag cagactccca acattggaac atcattttca
    12421 cgcgcatagc gaatggcttg gattttccct tctgtaccac gttgaccaaa accacctggt
    12481 acgatgatcc cgtctgcatc agacaagagt tctgctacat tctctgctgt cacatcattg
    12541 gcattgaccc aattgatttt aacttctgca tcattgacat agccagagtg tttcaaggct
    12601 tcgaccactg agatataggc atcttgcaac tccacatact taccaacaag ggaaatctta
    12661 acttgtttct tgaggttcat gaccttgtcc accatggctg accattctgt catagccgct
    12721 gctggtgcgt ctaatttcaa atgatcacaa acaatttggt ccatcccttg tgcctgcaag
    12781 ttcagtggaa tttggtaaag gtgttcaaca tccaacgatt cgataacggc ttctggtgcc
    12841 acatcacaga actgggccag tttattttta attccttgac cagctggctc ttctgtacga
    12901 ataaccaaca tatttggttg gattcccaag ccacgcaatt ctttgacaga gtgttgggtt
    12961 ggtttggttt tcatttcacc agcagccttg aggtaaggaa gcaaggttgt atggatatac
    13021 atgacattat ccgcacccac atctgccttc atctgacgaa gagcctctag gaatggcaag
    13081 gactcgatat ctcctactgt tccaccaacc tctgtgataa tgacatcaga gtcggtcgtt
    13141 agagcggcac gcttgatttt ttctttcaaa gcatctgtga tatgaggaat gacttgaaca
    13201 gttgccccaa ggtattctcc acggcgttct ttacgaagaa cttcactgta aattttccca
    13261 gttgtcacgt tggaatattt gttgagattg atatcgatga aacgttcata gtgacccaag
    13321 tccaaatctg tctcagctcc gtcatctgtc acaaaaactt ccccgtgctg gtaaggactc
    13381 atggttcccg gatcaatatt gatataaggg tcaaactttt gaatggttac tttgagacca
    13441 cgatttttca agagacggcc tagactcgct gccacaatcc ctttcccaat agacgatacc
    13501 acaccaccag ttacaaaaat atatttcgta gacatagatt cctctttcta aaatgctcaa
    13561 ggtcttgtta actacgaggg gatatagaaa acaagaccct actaataaga ataatctggg
    13621 acgactgcac cagattcaca gctaatactc ttcgaaaatc tcttcaaacc gcgtcaacgt
    13681 cgccttgccg tagatatgtg ttcctgactt tgtcagtctt atctacaacc tcaaaatagt
    13741 gttttgagca gcctacggct agtttcctag cttgctcttt gattttcatt gagtataaaa
    13801 tacaagaaga taacttcttg aaaaaacaaa aatagctccc taagaactag ggagccccga
    13861 cctctaaaag aggtgcccaa acaatatgat acctaaaaat aggataattg tcaatagcta
    13921 actcttattc ctcgctggtt tcagcatcgt catctgaaaa ctcgtcgtcg tcttcgttga
    13981 gatccacatc ttctcccaag tcatctggag cgatttcgtt gatttctgca tcataagctt
    14041 ccacttcatt tttttcatca tctggatttt catcatcgta tgaaagagct ggatctgctt
    14101 cgtatgcatc ttcgtcttct ggatcatctg cattgtagtc aatggcatct gaatcaccat
    14161 ccataaaggc attgacacgt tttttcttag cttttggtgc tacttcatcg tcgtcatttt
    14221 cttcaagagc gatgatttct tcgtcgattt cgtccacacc ataccatgaa cgaagacccc
    14281 atttgttgtc cccaagtgag atgaagctac cgtcaaagtt caactctgtg tagaacaaag
    14341 gcaaagcttc gcggatatcg ctgtttgatg ttccaaggta gttttgaatt tcgtttacaa
    14401 gatcgctaaa atgcatctca tgatcgcgac cacgaagttc caagatagca cgcgctacct
    14461 caatcataga tagttcactt ttttcttgcc cagcaaatac ttctaattcc aaagcgtttc
    14521 tcctcattta tactactatc gccagagcga acagactctg acctcatttt atcatttact
    14581 ctttatttta cgataatttt gcggaatagt caaaggttaa gggggagaaa gtggcaggat
    14641 tagactaatt ccaatataaa actccttcct ttttctgttg ctccattttc cacaaatcca
    14701 agcgacttga aacacctcct agaagcatga ttgtaggtgt agatttcctt gactctcaat
    14761 tctttccatc cttttactcg agccaattca atcaaagcac ttagaatctt ttttccaagt
    14821 cctcgatgtt ggtaagcgga attcccaatc acaatgggga gattatcctg agatagtgta
    14881 atatccccaa ttggaaacca ttctcccttc tccttgactt caatccaaaa aagctcacca
    14941 tgccgatcca aataggaata catggcttcc aaggtcgctt gactgtaagg aagcttcacc
    15001 ccatctacga ggtaaaccaa gttcacatcc tgataccaag caaaagcttc ctcacaatga
    15061 ttgaccttat aataaggaac aaaacacaat gcactacctt ttcaacaaaa gagttgctgc
    15121 ttgattaaaa ccatcacacc agttatacca ttttgcttca tacccatctt gagctaggat
    15181 acgatcttct aaatcaaaaa cagagtaaat ctttctttcc tcgcaagctt gcgcatagag
    15241 atgatatagt tcatcaccac catctctatc ccactcagca gaaatcgtat cccgacctgc
    15301 caataaagcc tgataagccc tgtgatgccc atctgtaatc agcaaacaat ctccaaaggc
    15361 aagaatactg attggatcga cttggattgt ttctgccgac tggtaaagca tctgaatatc
    15421 ttgcaacttc ttttctgata aatatagttg agtcggatga agatctgcta tattgacttt
    15481 catttctttc tcctcaaggg aattcgatac tcacttctgt ttgcctttaa atcgccattg
    15541 gaagcggagc ttgtcataga agggaaactc gataaacagg actcccaagc ccacacagag
    15601 actggcaagg acgtctgatg ggtaatgaac tcccagatag actcttgata ccagcacact
    15661 gactaggtag aggccaagga cgatttgtac gatttttctc cagactggat ttttaattcg
    15721 ttgactgaga atgacaatca gagtacctac catcaaagtt acagccagag aatgcccact
    15781 tgggaaggaa aatcccttct cttccaccag atgtaagata gctggtcgtg ggcgctggta
    15841 gatattttta aaggtcacga ttaaaagacc tgccaaagcc agatttccca gcatgaagaa
    15901 actttctatc ttccatcgct tacgataaaa gacaaaagct gtaatgacaa cccaagtgat
    15961 aatcactggg atatcaatca gacgtgtgag ggctcgaaaa agaatagtca aataatctgg
    16021 taagtctcct cgaatggcag tctgaatcga ttggtcaaaa ttgaccaaca tttcagggta
    16081 aaatttgacc atatagccaa gaataacgaa aagtaaaagg gcaaaactgc ccttcattaa
    16141 aaatgtttgt ttatctttca tagtgtttta aggttggttt caaaaggacg tacaacaacc
    16201 agaatgaaac ggaaaagatt acaccctcaa tcaagttaaa aggtaatacc atggtcatta
    16261 ggtagttgga aagtcccaaa atttttccaa tatcaaagtt agcaaactta gcgtacaaag
    16321 gaacagcata aacatagttg agaaccaaca tggccaaggt taaaccaaca gttccagcta
    16381 gagagcctag taggaaacga agggttgtcc gttccttttt ccaaatcaaa gcaaatacga
    16441 tgacaaaaac tcccaaagct acgatattca tcggcaaacc aatgtaagta ttcactcctt
    16501 ggctgttaag aagcaatttc aatagtgagc gaagcaagag cactcctaga gcagcaggca
    16561 aatccatgac caccagaccc acaaggactg gcaagatact aaattcgatc ttgaggaaag
    16621 atgccgctgg taaaagcgga aagtcaaagt acatcagcac aaatgagatg gctgataaaa
    16681 ttgcaatggt cgaaagtcga cgtgtgtttg tcataacagg ttcctccaat tttctataaa
    16741 atcagaagaa gttggaaagg attcctctat ctattctcac tttttatatc ccaaaagttc
    16801 cctcttactc tattaaagaa aaacaaagca agtggttaca atccggctat aaatctatca
    16861 aaacagacaa agctattctt tcgtcttctc ccatccagac tatactgtcg gttgtggaat
    16921 ctcaccacat cagcttgcgc tcgcggactt ctttaaagag aaggaaatag agatttttct
    16981 tttagactag gcgacaagct gtaggcataa cttctggtta ggcaaagctt gtcaacaaag
    17041 tataaaagga aaagatcatt tcctagttaa agtcaccgcc ggtcgggaat cacaccctgc
    17101 cctgaagact cttttatcat aacaaaaaac gcttgcaagc gcaagcattt tgttcatttt
    17161 cattttttat ttcaatttat ccacttcata ggtatggacg agctcaagac ctgcaaagtt
    17221 ctgctggcga agagcctcat agacaatcat gcagacggta ttagacacat tgagactgcg
    17281 gacatgttca tcattcatgg gaatacggag agccttctca ggatgttctc gcataaaatc
    17341 ttcaggtaag cccttgtctt cacgtccaaa gagaaaataa tggtcctcgt cagtcgataa
    17401 atccacctca gaatagactt tttccgcgaa tttcgaaatc agatagagtt ttcccttcat
    17461 ctgagatatg aaatcttcca aactctcgta aaaataaatc tctagtttat cccagtaatc
    17521 caatccagcc cgcttcatct tgcggtcatc aataggaaag cccataggct tgatgatgtg
    17581 gaggggagaa ttggtcgcag cgcaagtacg cgcgatattg cccgtatttt gtggaatttg
    17641 aggttcaaat aatacaatgt gatttgtcat gacttgcttc ctttcaccat tgcaaaaaaa
    17701 tagccacact gcccggagtc aagctcagca aacagcgtgg ttaaggcatc gttaacttac
    17761 ctcacaacag gtttgaagta aatcagcgaa actactttct tagtataaca ctttcagaat
    17821 cattgtcaat agaaatgact tgattttttc aattttttca agctatttcc aagggttgta
    17881 aaatcgtccc tgattctgca agataagtag taaactaact actaaaaaca aggttgccaa
    17941 gagcaaggta atatagtctc cttttttcaa ggcctgataa ctataccatg tgcgtttttt
    18001 ctctttccca aagcggcgaa gctccatggc agtcgcaatg gtatcaatgc gttctagcga
    18061 gctaaaaatc aagggcgtaa taatgagcag attgcctttg attcgttgca taagagaagc
    18121 tttcttggat aattccatcc cacgcgcctc ctgagacatc ttgatagtaa agaattcttc
    18181 ctgcaaatct ggaatatagc gcaaggtcag gctgacagaa taagcaatct tatagggcac
    18241 accaatttga tttaaactgg aagcaaactg actaggatgg gttgtcatca aaaagataat
    18301 agccagagga atggtgcaaa gatacttaat ggccaaattt agcagataaa agagctcctg
    18361 gctggttaga gtgtagacac cgattccctg ccaaatcaca cttctctctc cataaagtcc
    18421 aaccccatac tcgggagaaa agagatagac catcaaaacg tttaaaacgg caaataccgt
    18481 cgcaaaaacg gctacaaagg agacatcttt aaagcggatt tctgataaat agaggagaaa
    18541 gactgaaaag atggcaatca gcaagagcat tctggtatca tagctaatca tggccgccaa
    18601 tgataccaga atgaaaaaga gaagtttccc agctcctgac aagcgatgaa tcacagtatc
    18661 tctatgctgg taaccgatta atttagcttg catccctctc tcctttcttt gtaaaatgcc
    18721 gttaaatcca gtggatccac atctagtttc ttagccaagt taaagatgga ggtttctttt
    18781 agattggctt ttactaacag ctcaggatcg ctcaacagac tggctggaac agtatcggca
    18841 atcaattctc catccaccat gacaaggacc cggtctgaat aatccagcat caattgcata
    18901 tcatgggtaa tcatgacaat ggtatgccct ttttgatgta actcttcgag aaattccata
    18961 atctcagtat agttcttctg atcttgacct gcagtcggtt catctaggag aataatttca
    19021 gctcctaaga ccaaaattga agcaatggtg acacgttttt tctgaccaaa tgacagggca
    19081 gaaataggcc aattacggaa ttcataaagt ccacagattt tcaaggtttc atagactctc
    19141 gtttcaattt ccttctcatt cacacctcgc aaacggagcc ctagagccac ctcatcaaaa
    19201 atcatattgg ttgaaatcat ttgattagga ttttgtagca catagcctac tcgttccgcc
    19261 cgctctgcaa cagaatcgcc ttttatatcc tgtttttccc aaagatagcg tccttccgtc
    19321 tgaataaagc tacttatagc cttggctaga gttgatttcc ctgctccatt ttttccgaca
    19381 atagcaatct tttcaccctt tttaatatct aaatgtaggg attttaaaat cggtctatca
    19441 ttataagaaa aagatacttc ctctagtata aagagtgact gcaatgctgg ggtttctttt
    19501 accagttccg tctgtaactg gatttgccct tttgagatag acaagttatc cagatctgct
    19561 aattgttctt ccttgactaa gtccacacct aattgacgga gagtcgttag ataaaggggt
    19621 tctcgaattc cattttgagt caataaatca gtcgcaagca actggtcagg gctcccatta
    19681 aaaaggatac gaccatcgtt tatcaagaca atccgatcca cagggcgatg cagaacgtcc
    19741 tccaaacggt gctcgataat aagagtcgtc gtcccctctt ccttatgaat ctggtcaatc
    19801 aattcgataa tatcctgacc tgacttggga tctagattgg cgagtggctc atcaaacaag
    19861 agaatcggac tttcatcaat caagacacca gccagactga ctcgctgctt ttgtccacct
    19921 gacaaatcct gaggacgctg atccagtaaa ggaagaaggt ccagcttttc agcccattta
    19981 taaacacgac ctttcatctc atctagggct gtcacatcat tttccagagc aaacgccaaa
    20041 tcttctgcca cagacaagcc aataaactgc ccatctgtat cctgcaaaac tgtgctaacc
    20101 agatgagact tatcatagat gctcatatca aaggctgttt gaccctttat caaaaattct
    20161 ccatatgtct gacccttgta aatattggga ataatcccat tcaaacactg acccaaggta
    20221 gatttacctg atccagatgg tccaacaatt aagactttct ctcccttgta aatggtcaag
    20281 tctatccctt gcaaggtcgg ttcttgttgt gtttcatacc ggaaagagaa atccttccac
    20341 tcaattatag cttctttcat cttactctct tcattcgctt cttagacttc tattttatca
    20401 taaatcaagc ccttcttgca gtctcttctc ttaaaatctt agcgccaaaa agattcctat
    20461 cctagcttac ttgcctaact aatctataaa catcgaaaaa gactagttgc ccagccttcc
    20521 ccatcatttt atactcttcg aaaatctctt caaaccacgt cagcttcgcc ttgccgtagg
    20581 tatggttact gacttcgtca gtttcatcta caacctcaaa accatgtttt gagctgactt
    20641 cgtcagttct atccacaatt tcaaaacact gttttgagca acctgcggct aacttcctag
    20701 tttgctcttt gattttcatt gagtattagt cctttttcaa acttcctgca cgagtttggg
    20761 ttcctgcata ggcaagtaag agaagagttc ctgcaatagc tacagataca ccattggcaa
    20821 ttcccgcaac aatcccttgt gcaaatactt tttctgccgc ttcttgataa atcacaacat
    20881 ctccaagtgg tgccaagaca ccccaaacaa gggcatttgc aagtagttga atgaggttaa
    20941 aaataagaat atctttccag tcaaaaacac cattgatcac gcgaacgtac tttctaaaaa
    21001 gtcccacaac taaaccaaag agtccgctag cgataatcca agtccaccat agaccataac
    21061 cagcaagaga gtccttgatt gcatgaccaa tcaacccgac aagcaaaccg ataatcggtc
    21121 caaaaataat agaaagtagc gcttgtaccg catactgaag ctggatgctt gtatttggaa
    21181 caggggttgg aatgttgatc atcccgatga cgacaaagag ggcagcgcca attccgacag
    21241 caacaacttg tttaattgta aatttaattt ccatactatt ctcctatttt atccttctat
    21301 tttctttatt tcaatggtcc aagatgaacc gacacctaca ttataggcct tggcaaagga
    21361 accttggttg atagccaaac ctaaacgata gagagagttg atgtaaagga tgggttgccc
    21421 aattctcaca tctgcaaatg atttgccata gacaacctga ttttgataga ccagcatatc
    21481 agcatgatag atggtcactt caaaacgatc accaaattct ggttccagct tgtaaaattc
    21541 ttcccgtgtg atagaggtcc aaagcgaacc gaaacgcaca tccagaatat caatggctcc
    21601 cttcaccaga tgatcttcta tgatggtcgc tacgactgga agctctacaa tctgttccac
    21661 actgagctct ggccctactt cctcaaaagt aatgtgacca ctggccagtt tagcaccagt
    21721 ataggcatag acatcacgac cgtggaaggt ataagaatgc tctgtgtttt gacgcctatt
    21781 ggccacctca gaaatctcac gaatggctac aatgccaacg tgtttcttga taaaggaaag
    21841 cgtcccatta tctggcgtga caatgtattg attttttgca gtcttggcaa ctacactctt
    21901 acgtttcgaa ccgacacctg gatcgacaac cgatacaaac gtcgttccct caggccagta
    21961 atccaccgtc tgaaagagac gatagctccc ctcaaaaata ttataaggcg tgatatcgtg
    22021 cgtcaagtga tggattttta aggttggaga ctcttctaaa gccactccaa tcatagccga
    22081 taccgcacca tcaaccagac caaagtctga ttgtaatacc agtaaattat tattcatttt
    22141 atctccttgt atatccttta tcataccata tttcagagaa agtcgctaat agctatttca
    22201 attggttagg gtatagtttt accttatagc gaattttcac taaaagctct tgttattagc
    22261 tagtaggtgc attttttctt gaaaaagggt atgatagtta catcatgaaa aagtaggttt
    22321 tattatgaaa attatccttg tcggaggggg aaaagttggt tttgccctct gtcgctcctt
    22381 ggttgcagaa aagcatgatg ttttgctgat tgagcaagac gaagctgttc tcaatcatat
    22441 tgtcagtcgc tttgatatca ttggtatcct tggtaacggg gccgattttg ccattcttga
    22501 gcaagccagc gtccaagatt gtgatatctt tatcgccctg actgagcacg atgaagtcaa
    22561 catgattgca gcagttctag ccaagaaaat gggagctaaa gaaactatcg ttcgggtgcg
    22621 gaaccctgaa tattctaact cttatttcaa ggaaaagaat attctcggtt tttctcttat
    22681 cgttaatcct gagctcttgg ctgcccgcgc tatcgcgaat atcattgact tccccaacgc
    22741 cctgtctgtc gaacgctttg ctggtggacg cgttagcctc atggaatttg tcgtcaagtc
    22801 caccagcggt ctttgccaaa tgcccatttc tgattttcgt aaaaaatttg gtaatgtcat
    22861 tgtctgtgcg atagagaggg atcatcaaat tatcattcca agcggtgaca tgactgtaca
    22921 ggataaagat agaatctttg tcactggtaa ccgtgtcgat atgatactct tccataatta
    22981 ttttaaatca cgcgccgtga agagccttct catcgttgga gcaggtagaa ttgcctatta
    23041 tctacttggt attctcaaag atagtcgtat cgatacaaaa gtcattgaaa tcaatcctga
    23101 aatcgccagc ttctttagcg agaaattccc aaatctctac atcgttcaag gagatggaac
    23161 cgcaaaagat atcctgctgg aagaaagtgc tcaacactat gatgccgttg cgactctaac
    23221 aggagtcgat gaggaaaatc tgattacatc tatgttcctt gacagggtag gtgtacagaa
    23281 aaatattact aaggtcaatc gtaccagtct cctcgagatt atcaatgcgc ctgatttttc
    23341 aagtatcatc acacctaaaa gcatcgctgt agatacgatt atgcacttta ttcgtggtcg
    23401 agttaatgcc cagtattcag accttcaagc catgcaccat ctagccaatg gccaaatcga
    23461 aaccctgcaa ttccatatca aggaagccaa taaaatgact gccaaacctc tttctcaact
    23521 gaaattgaaa aaaggggttc ttattgcagc cattattcga aagggcaaga ctattttccc
    23581 aactggggag gatatgttgg aagttggaga caagctccta gtaacaacct tgttgccaaa
    23641 catcaccaag atttatgact tgattgcgag gtaagaaatg aataaaagta tgattcgtta
    23701 cctcctttca aagttacttt tgattgaagc tgttctcctc ttggttcctg tggctatcgc
    23761 tgtctattac cgtgaatcga gccaagtctt tacagccctc ttttcgacca tagggattct
    23821 cgtattgtta ggcggttcag ggagtttaca gaagccaaaa aatcaacgga tttatgccaa
    23881 ggagggagtc ttgatcgttg ccctctgttg gatcctttgg tctttctttg gtggtctccc
    23941 ctttgtcttt tctgggcaaa tccccagcct tattgatgcc ttttttgaga ttagttccgg
    24001 ctttacgact actggagcaa gtattttgaa cgacgtttca gttctcagtc gttccctcct
    24061 cttctggcga agttttaccc acttgattgg agggatggga gtgcttgttt ttgcacttgc
    24121 tattatggac aatgccaaaa atagccacct agaggtgatg aaggctgagg ttccaggtcc
    24181 tgtttttggc aaggttgtat ccaaactaaa aaacactgcc cagattctct atctccttta
    24241 tctagctctc ttctccctct ttgtcatcat ctattatcta gctggtatgc ctctctacga
    24301 tagttttgtc attgctatgg ggacagcggg aactggaggc tttaccgtct ataacgacgg
    24361 aattgcccac tatggcagct cactgattac ctatctggtc agtatcggag ttctggtttt
    24421 tggagtaaat ttcaacctct actactacct catgctccgt cgcatcaagg ccttctttgg
    24481 agatgaagag cttagggctt acttggtcat tgtactggtt tctacaggct tgattagcct
    24541 caacaccctc tacctctacc cagggttttc aaagagcttt gaaatgacct tcttccaggt
    24601 ttccaacatc attacaacaa ctggttttgg atatggagat attaccaact ggcccctctt
    24661 ctcccagttt atcctccttt tcctcatggc aatcggtggc tctgctggat caactgcagg
    24721 tggactcaag attattcgag gcctcatcct ttcaaaaatt gccaaaaacc aaattttgtc
    24781 aatcctatcg ccccaccgtg ttttgaccct ccatgttaat aaaacggtga ttgacaaaga
    24841 tacccagcat aagattctca agtactttgt catctatgct atgattttgc tagcgcttat
    24901 ctttattgtc agcctagata gcaatgattt tctggtcgtg accagcgctg tctttagctg
    24961 tttcaataat atcgggccta ttctaggaac cacttctagt ttctcaatct ttagtcccat
    25021 ctcaaaaatt ctcctatcct ttgcaatgat tgcagggcgc ttggagattt acccaatcct
    25081 ccttctcttt atgaagagaa cttggtctaa gagataaaat acaaaatccc tctttttaca
    25141 attgtaaaaa gagggattct tatattaaaa aagggggaac gtttttaaat ccaagcctgt
    25201 ctgtaagcaa agtccttact caaataaaag atggaaaaac ttactacaca gcttggtaat
    25261 gctccaatac aaattgtaag gatttacttg gattctttgt taaatgaata tattccattc
    25321 ctctggtagc aactatctga atacctaatc tttctgcatc caccttcatc tctctataat
    25381 aactccgaac ttgtggggcc agaataatta aatcataaac acccataata tcataatgag
    25441 ctccgtacgc tcctgaattc gcaatcactc taacctctgt taagttagcc ccttgttaat
    25501 tgcattggct aattgcgcac ttgttccaga ccctgcacaa agaaccagta ctttcaactc
    25561 cttacttttt atttcatcta actcaccagg cattatttca cttgtatcct cctctaaaat
    25621 aatatcattt gagcttgcaa tatctataac ctgtagtaga tatctcgtat ttagacaata
    25681 tgaaaacaag acgacttcca tataggaaac cgccttctcg ctatgttgag tgatttatat
    25741 taaaataact tttcttctag ctgcatttta ttattataaa aacattcatc ataaccccca
    25801 gaacttaaat aacaattttt attcaagata catactccta gaataaactt tatatgaaat
    25861 tctcattttt gtttttacaa ttctccttag ttaaatcttg tttaatatat gttttacata
    25921 tagtatttag cgccacatag tactgaactc tctccaaaaa cggttatgcc tctttgaata
    25981 gggcgttatc acaagaaaag catctccacg tttcaacttc atatggctca aaaacaatca
    26041 attgatgcta aaacctgtat ctagatgttt cggttcataa aaccatgaaa ctgtaaaagt
    26101 ggatgaaatt gatagcgata gtcaaatcaa gaggcatcat aactctaaaa agtcacaata
    26161 tataagttca tcctcggaaa aatatcattc taattattga aatgcctaca tgaaaagaaa
    26221 cgttaaatgc tcatgaaaca acgaatacag gtatcaaaac tgtgacaaaa caagtcccta
    26281 aatttactaa agacactgct caactttaca cctgtaaatg gttgttgtat aataaagtta
    26341 caaagatgta cgaccacact gttgtaaatc atagtgttcg cgaatatatt actgatagca
    26401 tttctacaaa tacaattgaa gagattggat aggctttaaa cgaaatatgt cagtgctttg
    26461 gcattcctag ccttcatatc atttaaagaa ttctatagac aaaatttttt ccaatacaga
    26521 cactcgtaac aactgcttca tttttctacc aacatattta ggaacaggat aagatacaag
    26581 agtattaatc catagctcag ttctatacca atctaagaca aataagctaa aaaaacgatt
    26641 gataataagc aaatagattc caaattttct ctatctgctc attttaataa acaatactag
    26701 tgtaactatc cttccagtca gaagcttgtc aaatcacacc gaaaattctt ctaaaattta
    26761 tctcgttagg caatcaagca aaaactcgac gatagtacaa acattatcat acaggattga
    26821 cttcctaaat tatatacttt agtaaggttt tcggataaga aaaaaggttc attttacatt
    26881 tctaaacatt cttttctaag atgaaaaaca gaatttttcg attgtgattt aaagcaacaa
    26941 gaagattttc agtatcatcc tatagatacg agctaattaa gaaaaactac atttttgaat
    27001 ataaactaca ataatataaa ctaaatttta taggaggaag acaatggatt ggtacgatta
    27061 tatgatacag gcatccaaac aatcacaatt caacgcaagc cattggtttc gctatttgcg
    27121 aaaagttatt tttgaagact attcttattt aacaaaccaa gatgtagaaa agttgctaga
    27181 ctccaaagaa ctaacccgtt ttcaaaaaat tagcttgaag tatgcctttc aagagcatac
    27241 tccaactcat aaatatgtga tttcattaaa taaacctgct aagttaacca atgttcaaaa
    27301 attgatggag aaatacaaac atggataaaa tgaaaccggt cttccaagcc ctaaataagg
    27361 aattaattca ggaaaatctg actttaacaa ttatctgtgt cggtggttat gtcttagaat
    27421 atcatggttt acgtgccaca caagatgttg atgcttttta tgaccaaaat cagaaaatca
    27481 atgaaattat tgctcgtgta ggaaaacagt ttaatcttaa cacccacgaa gagttatggc
    27541 tcaacaatca tgttgcaaat atgaacaaac aacctccctt aagtttatgc gaatccttgt
    27601 actcatttga gaatctgacc gttttagtag tccctattga gtatgtgtta ggaatgaaga
    27661 tgatgagtat cagagagcag gatttgaaag atattggtgc aattattaaa tacaaaaact
    27721 ttcattcacc attcgatacc tttaaatatt taaaagacat gggatttgat actattgacc
    27781 tctctgttct attagaaggt tttagttatg cttatggtat ggattggtta gaaaaatttt
    27841 ttaaagaaaa tcaagataaa cttcgcgaat tttattaaca gtatcaaaca agatgattgt
    27901 caattagcca gtcgtcttgt ttttatgatg aaatgaaata aaatcggaac aaatcactta
    27961 gagaattcca gtcaatttct agcactgttt gagacgtttc agcggactat agcaaaatga
    28021 aataagaata ggacaaatcg atcaggacag tcaaatcgat ttctaagaat gttttagaag
    28081 taaaggtgta ctattctact ttcaatctat tatatttcag attcggtcca ctattccttc
    28141 ccaaaaacat tgcatattgc gccgaaggaa ttgctcatac tatcccaagc acaaaaccct
    28201 cagtcgtttg accgagggtt cctttttatt attcaagaac ttgaggctct ataatatttg
    28261 tagtgggtaa atcccctatg gatattatgg agcctatttt
//
LOCUS       DAVDWT010000026        26372 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVDWT010000026 DAVDWT010000000
VERSION     DAVDWT010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.026"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..984
                     /gene="lytB"
                     /locus_tag="VUW50_001417"
     CDS             <1..984
                     /gene="lytB"
                     /locus_tag="VUW50_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HET7983301.1"
                     /translation="SDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSDGK
                     WLGGKTTNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAITIS
                     GLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKYYS
                     ADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAEEH
                     YHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILGAT
                     KWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(1045..2700)
                     /gene="pavA"
                     /locus_tag="VUW50_001418"
     CDS             complement(1045..2700)
                     /gene="pavA"
                     /locus_tag="VUW50_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HET7983302.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKQYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            2805..3302
                     /gene="ybeY"
                     /locus_tag="VUW50_001419"
     CDS             2805..3302
                     /gene="ybeY"
                     /locus_tag="VUW50_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET7983303.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRNTDRPTDVISLEYKPELEIAFDEEDLLENSELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            3283..3678
                     /locus_tag="VUW50_001420"
     CDS             3283..3678
                     /locus_tag="VUW50_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET7983304.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFALTGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            3695..4594
                     /gene="era"
                     /locus_tag="VUW50_001421"
     CDS             3695..4594
                     /gene="era"
                     /locus_tag="VUW50_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET7983305.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            4643..5467
                     /gene="mutM"
                     /locus_tag="VUW50_001422"
     CDS             4643..5467
                     /gene="mutM"
                     /locus_tag="VUW50_001422"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HET7983306.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDVYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            5467..6072
                     /locus_tag="VUW50_001423"
     CDS             5467..6072
                     /locus_tag="VUW50_001423"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET7983307.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPDEREWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            6059..7258
                     /locus_tag="VUW50_001424"
     CDS             6059..7258
                     /locus_tag="VUW50_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HET7983308.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            7258..7407
                     /gene="rpmG"
                     /locus_tag="VUW50_001425"
     CDS             7258..7407
                     /gene="rpmG"
                     /locus_tag="VUW50_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET7983309.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            7447..7680
                     /gene="secG"
                     /locus_tag="VUW50_001426"
     CDS             7447..7680
                     /gene="secG"
                     /locus_tag="VUW50_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET7983310.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            7782..10136
                     /gene="rnr"
                     /locus_tag="VUW50_001427"
     CDS             7782..10136
                     /gene="rnr"
                     /locus_tag="VUW50_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HET7983311.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            10099..10566
                     /gene="smpB"
                     /locus_tag="VUW50_001428"
     CDS             10099..10566
                     /gene="smpB"
                     /locus_tag="VUW50_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET7983312.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQEIKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            10582..11442
                     /gene="tehB"
                     /locus_tag="VUW50_001429"
     CDS             10582..11442
                     /gene="tehB"
                     /locus_tag="VUW50_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HET7983313.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTVVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIGQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            11515..12468
                     /gene="coiA"
                     /locus_tag="VUW50_001430"
     CDS             11515..12468
                     /gene="coiA"
                     /locus_tag="VUW50_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HET7983314.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQIHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            12487..14289
                     /gene="pepF"
                     /locus_tag="VUW50_001431"
     CDS             12487..14289
                     /gene="pepF"
                     /locus_tag="VUW50_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET7983315.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTFYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            14291..15004
                     /locus_tag="VUW50_001432"
     CDS             14291..15004
                     /locus_tag="VUW50_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET7983316.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMVEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            15071..16012
                     /gene="prsA"
                     /locus_tag="VUW50_001433"
     CDS             15071..16012
                     /gene="prsA"
                     /locus_tag="VUW50_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HET7983317.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKSNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(16129..16470)
                     /locus_tag="VUW50_001434"
                     /pseudo
     CDS             complement(16129..16470)
                     /locus_tag="VUW50_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            16883..16978
                     /locus_tag="VUW50_001435"
                     /pseudo
     CDS             16883..16978
                     /locus_tag="VUW50_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(17231..17851)
                     /locus_tag="VUW50_001436"
     CDS             complement(17231..17851)
                     /locus_tag="VUW50_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7983318.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(17851..18333)
                     /locus_tag="VUW50_001437"
     CDS             complement(17851..18333)
                     /locus_tag="VUW50_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HET7983319.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(18333..19175)
                     /locus_tag="VUW50_001438"
     CDS             complement(18333..19175)
                     /locus_tag="VUW50_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HET7983320.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(19270..20070)
                     /locus_tag="VUW50_001439"
     CDS             complement(19270..20070)
                     /locus_tag="VUW50_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HET7983321.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSAQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            20214..21593
                     /gene="glmU"
                     /locus_tag="VUW50_001440"
     CDS             20214..21593
                     /gene="glmU"
                     /locus_tag="VUW50_001440"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET7983322.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVILKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            21603..22148
                     /locus_tag="VUW50_001441"
     CDS             21603..22148
                     /locus_tag="VUW50_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7983323.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            22160..22474
                     /locus_tag="VUW50_001442"
     CDS             22160..22474
                     /locus_tag="VUW50_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HET7983324.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            22491..23183
                     /locus_tag="VUW50_001443"
     CDS             22491..23183
                     /locus_tag="VUW50_001443"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HET7983325.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            23260..23751
                     /gene="rocS"
                     /locus_tag="VUW50_001444"
     CDS             23260..23751
                     /gene="rocS"
                     /locus_tag="VUW50_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HET7983326.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            23849..24439
                     /locus_tag="VUW50_001445"
     CDS             23849..24439
                     /locus_tag="VUW50_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET7983327.1"
                     /translation="MKRLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            24448..26211
                     /locus_tag="VUW50_001446"
     CDS             24448..26211
                     /locus_tag="VUW50_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET7983328.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         7996 a         4796 c         5800 g         7780 t
ORIGIN      
        1 tctgatggca aaatggctga aaaagaatgg gtctacgatt ctcatagtca agcctggtac
       61 tacttcaaat ctggtggcta catggcgaaa aatgagacag tagatggtta tcagcttgga
      121 agcgatggta aatggcttgg aggaaaaact acaaatgaaa atgctgctta ctatcaagta
      181 gtgcctgtta cagccaatgt ttatgattca gatggtgaaa agctttccta tatatcgcaa
      241 ggtagtgtcg tatggctaga taaggataga aaaagtgatg acaagcgctt ggctattact
      301 atttctggtt tgtcaggcta tatgaaaaca gaagatttac aagcgctaga tgctagtaag
      361 gactttatcc cttattatga gagtgatggc caccgttttt atcactatgt ggctcagaat
      421 gctagtatcc cagtagcttc tcatctttct gatatggaag taggcaagaa atattattcg
      481 gcagatggcc tgcattttga tggttttaag cttgagaatc ccttcctttt caaagattta
      541 acagaggcta caaactacag tgctgaagaa ttggataagg tatttagttt gctaaacatt
      601 aacaatagcc ttttggagaa caagggcgct acttttaagg aagccgaaga acattaccat
      661 atcaatgctc tttatctcct tgcccatagt gccctagaaa gtaactgggg aagaagtaaa
      721 attgccaaag ataagaataa tttctttggc attacagcct atgatacgac cccttacctt
      781 tctgctaaga catttgatga tgtggataag ggaattttag gtgcaaccaa gtggattaag
      841 gaaaattata tcgatagggg aagaactttc cttggaaaca aggcttctgg tatgaatgtg
      901 gaatatgctt cagaccctta ttggggcgaa aaaattgcta gtgtgatgat gaaaatcaat
      961 gagaagctag gtggcaaaga ttagtactat aagtgaatat gatttgagtg aatagtaagt
     1021 taaaaatcct gatttcaagt aaaatcagga ttttttcatg gatgcaattt ttttggagtc
     1081 tggtgtgacg cggagggtct tttgtcctgt gtaagtgaca aagccgggtt ttccaccagt
     1141 tggtttattg agttttttga cttcaatcat atctacctgc accagattcg acaggcgccc
     1201 ttgagagaag taggcagcta actctgctgc gtctgtcttg actgcatcag atgggtcaag
     1261 atttcctgag atgacaacat ggcttccagg aatgtcctta gcatggaacc aaagttcctc
     1321 cttgcgggcc attttaaagg tcaattcctc attttgaaga ttgtttcgtc cgacatagat
     1381 gatggttttg ccatcgcttg ctagatattg ttctagtttt ttgcgtttct ggattttctc
     1441 ccgttgtctt ctgcggataa aacctgtttg aatcaattct tcacggattt cagcgatttc
     1501 ttccagtcca gcttggttga ggacggtttc tacactttcc agatagagaa tagtggcttt
     1561 ggtttcttca atcaaatcag tcaagtattt gacagcttct ttgagtttct gatactgttt
     1621 aaaatagcgt tgggcattct ggttgggagt cagagcctta tcaagcgcaa tcatgatagg
     1681 ttggttggta tagtagttgt ctaggataac ctggtcttgg tcgttaggca cttggtggag
     1741 gaaggttgtc agcaattctc ctttttgacg aaattcttca gcgttgtctg tcgccagtaa
     1801 ctctttttcc tgttttttga gtttgtgtcg gtttttctga agttcatttt caacacgacg
     1861 aatcagttca ctggcctgct gtttgacgcg gtcgcgctca gccttatcct tatagtaggt
     1921 gtccaacaaa tcagaaagat ttgcaaaagg ctctcccacc tgatttgcaa aaggaactgg
     1981 actgaaggaa gtctcagtca agcatggctt ggtttcttga ttgaaaaaat ttcggaaagc
     2041 ggaaagtttt tcactaacca gtatcctttc caattcattt gccgtatcgc gtcccagacc
     2101 ttgaaagagg ctttgaagat tttttgctgt tagttcttgg gtttgcagga tttcaaagag
     2161 cttttcatcc ttgatagtaa aaggattgag agattttgta cttggcggag cgatataggt
     2221 cgatcctgga agtaaggtgc ggtagctatt ttgtgaaaag ccgacgtgtt tgataacttc
     2281 gaggatttta tgactgcttt tatcgaccag tagaatatta ctgtgtttcc ccataatttc
     2341 gataatcaag gtagcctgga tatggtctcc aatctcgttt ttattggaaa ctgtaatttc
     2401 cacaatacgg tcattttcca cttgctcaat cgactcaatc agggccccct gcaaatactt
     2461 tctcaaaacc atgataaagg tagaaggttg agctggattt tcaaaagtcg tttgggtcag
     2521 ctgaatgcgt ccaaaaactg gatgggcaga aaggagcagg cgatggcttt ggcgattgct
     2581 gcggatttgc aagaccaact cttgttcaaa aggctgattg attttctgga tgcgaccatt
     2641 cactaattcg cttcgcaatt cctcaactat gtggtgtaaa aaaaatccgt caaatgacat
     2701 cgttctctcc ttgtgattgt attccatagt attatatcaa aaaggtagaa taaaatcatg
     2761 gaaatgtggt ataataaagc caagtaaaga gaaacgagaa gcacatgtat attgaaatgg
     2821 tagatgaaac tggtcaagtt tcaaaagaaa tgttgcaaca aacccaagaa attttggaat
     2881 ttgcagccca aaaattagga aaagaagaca aggagatggc agtcactttt gtgaccaatg
     2941 agcgtagtca tgaacttaat ctggagtacc gtaacaccga ccgtccgaca gatgtcatca
     3001 gccttgagta taaaccagaa ttggaaattg cctttgacga agaggatttg cttgaaaatt
     3061 cagaattggc agagatgatg tctgagtttg atgcctatat tggggaattg ttcatctcta
     3121 tcgataaggc tcatgagcag gccgaagaat atggtcacag ctttgagcgt gagatgggct
     3181 tcttggcagt acacggcttt ttacatatta acggctatga tcactacact ccggaagaag
     3241 aagcggagat gttcggttta caagaagaaa ttttgacagc ctatggactc acaagacaat
     3301 aaacgaaaat ggaaaaatcg tgacttgata tccagtttag aatttgcttt gacaggtatt
     3361 tttactgcta tcaaggaaga acgcaatatg cgaaaacacg cagtgacggc tctagtggtc
     3421 atccttgcag gttttgtttt tcaggtgtca cgaatcgaat ggctctttct cctattgagt
     3481 attttcttgg tagtagcctt tgagattatc aactctgcta ttgaaaatgt ggtggatttg
     3541 gccagtcact atcacttttc catgctggct aaaaatgcca aggatatggc ggccggcgcg
     3601 gtattagtgg tttctctttt cgcagcctta acaggcgcat tgatttttct cccacgaatc
     3661 tgggatttat tattttaaac agtaagagga aattatgact tttaaatcag gctttgtagc
     3721 cattttagga cgtcccaatg ttgggaagtc aaccttttta aatcacgtta tggggcaaaa
     3781 gattgccatc atgagtgaca aggcgcagac aacgcgcaat aaaatcatgg gaatttacac
     3841 gactgataag gagcaaattg tctttatcga cacaccaggg attcacaagc ctaaaacagc
     3901 tctcggagat ttcatggttg agtctgccta cagtaccctt cgcgaagtgg acaccgttct
     3961 tttcatggtg cctgctgatg aagcgcgtgg taagggggac gatatgatta tcgagcgtct
     4021 caaggctgcc aaggttcctg tgattttggt ggtgaataaa atcgataagg tccatccaga
     4081 ccagctcttg tctcagattg atgacttccg taatcaaatg gactttaagg aaattgttcc
     4141 aatctcagcc cttcagggaa ataacgtgtc tcgtctagtg gatattttga gtgaaaatct
     4201 ggatgaaggt ttccaatatt tcccgtctga tcaaatcaca gaccatccag aacgtttctt
     4261 ggtttcagaa atggttcgcg agaaagtctt gcacctaact cgtgaagaga ttccgcattc
     4321 tgtagcagta gttgttgact ctatgaaacg agacgaagag acagacaagg ttcacatccg
     4381 tgcaaccatc atggtcgagc gcgatagcca aaaagggatt atcatcggta aaggtggcgc
     4441 tatgcttaag aaaatcggta gcatggcccg tcgtgatatc gaactcatgc taggagacaa
     4501 ggtcttccta gaaacctggg tcaaggtcaa gaaaaactgg cgcgataaaa agctagattt
     4561 ggctgacttt ggctataatg aaagagaata ctaagtagag gtaggctcat gcctgcttct
     4621 tgtttttaca gaaggaggac ttatgcctga attacctgag gttgaaaccg tttgtcgtgg
     4681 cttagaaaaa ttgattatag gaaagaagat ttcgagtata gaaattcgct accccaagat
     4741 gattaagacg gatttggaag agtttcaaag ggaattgcct agtcagatta tcgagtcaat
     4801 gggacgtcgt ggaaaatatt tgctttttta tctgacagac aaggtcttga tttcccattt
     4861 gcggatggag ggcaagtatt tttactatcc agaccaagga cctgaacgca agcatgccca
     4921 tgttttcttt cattttgaag atggtggcac gcttgtttat gaggatgttc gcaagtttgg
     4981 aaccatggaa ctcttggtgc ctgacctttt agacgtctac tttatttcta aaaaattagg
     5041 tcctgaacca agcgaacaag actttgattt acaggtcttt caatctgccc ttgccaagtc
     5101 caaaaagcct atcaaatccc atctcctaga ccagaccttg gtagctggac ttggcaatat
     5161 ctatgtggat gaggttctct ggcgagctca ggttcatcca gctagacctt cccagacttt
     5221 gacagcagaa gaagcgactg ccattcatga ccagaccatt gctgttttgg gccaggctgt
     5281 tgaaaaaggt ggctccacca ttcggactta taccaatgcc tttggggaag atggaagcat
     5341 gcaggacttt catcaggtct atgataagac tggtcaagaa tgtgtacgct gtggtaccat
     5401 cattgagaaa attcaactag gcggacgtgg aacccacttt tgtccaaact gtcaaaggag
     5461 ggactgatgg gaaaaatcat cggaatcact gggggaattg cctctggtaa gtcaactgtg
     5521 acaaattttc taagacagca aggctttcaa gtagtggatg ccgacgcagt cgtccaccaa
     5581 ctacagaaac ctggtggtcg tctgtttgag gctctagtac agcactttgg gcaagaaatc
     5641 attcttgaaa acggagaact caatcgccct ctcctagcta gtctcatctt ttcaaatcct
     5701 gatgaacgag aatggtctaa gcaaattcaa ggggagatta tccgtgagga actggctact
     5761 ttgagagaac agttggctca gacagaagag attttcttca tggatattcc cctacttttt
     5821 gagcaggact acagcgattg gtttgctgag acttggttgg tctatgtgga ccgagatgcc
     5881 caagtggaac gcttaatgaa aagggaccag ttgtccaaag atgaagctga gtctcgtctg
     5941 gcagcccagt ggcctttaga aaaaaagaaa gatttggcca gccaggttct tgataataat
     6001 ggcaatcaga accagcttct taatcaagtg catatccttc ttgagggagg taggcaagat
     6061 gacagagatt aactggaagg ataatctgcg cattgcctgg tttggtaatt ttctgacagg
     6121 agccagtatt tctttggttg taccttttat gcccatcttc gtggaaaatc taggtgtagg
     6181 gagtcagcaa gtcgcttttt atgcaggctt agcaatttct gtctctgcta tttccgcggc
     6241 gctcttttct cctatttggg gtattcttgc tgacaaatac ggccgaaaac ccatgatgat
     6301 tcgggcaggt cttgctatga ctatcactat gggaggcttg gcctttgtcc caaatatcta
     6361 ttggttaatc tttcttcgtt tactaaacgg tgtatttgca ggttttgttc ctaatgcaac
     6421 ggcactgata gccagtcagg ttccaaagga gaaatcaggc tctgccttag gtactttgtc
     6481 tacaggcgta gttgcaggta ctctaactgg tccctttatt ggtggcttta tcgcagaatt
     6541 atttggcatt cgtacagttt tcttactggt tggtagtttt ctatttttag ctgctatttt
     6601 gactatttgc tttatcaagg aagattttca accagtagcc aaggaaaagg ctattccaac
     6661 aaaggaatta tttacctcgg ttaaatatcc ctatcttttg ctcaatctct ttttaaccag
     6721 ttttgtcatc caattttcag ctcaatcgat cggccctatt ttggctcttt atgtacgcga
     6781 cttagggcag acagagaatc ttctttttgt ctctggtttg attgtgtcca gtatgggctt
     6841 ttccagtatg atgagtgcag gagtcatggg caagctaggt gacaaggtgg gcaatcatcg
     6901 tctcttggtt gtcgcccagt tttattcagt catcatctat ctcctctgtg ccaatgcctc
     6961 tagccccctt caactaggac tctatcgttt cctctttggc ttgggaaccg gtgccttgat
     7021 tcccggggtt aatgccctac tcagcaaaat gactcccaaa gccggcattt cgagggtctt
     7081 tgccttcaat caggtattct tttatctggg aggtgttgtt ggtcccatgg caggttctgc
     7141 agtagcaggt caatttggct accatgctgt cttttatgcg acaagccttt gtgttgcctt
     7201 tagttgtctc tttaacctga ttcaatttcg aacattatta aaagtaaagg aaatctagtg
     7261 cgagtaaaaa tcaatctcaa atgctcctct tgtggcagta tcaattacct aaccagtaaa
     7321 aattcaaaaa cccatccaga caagattgag gttttaaagt attgtcccaa ggaaagaaaa
     7381 gtaaccctac atcttgaatc taagtagatt ttatggtaaa ataataggga ttttaaggag
     7441 tttgatatgt ataacctatt attaaccatt ttattagtat tatctgttgt gattgtgatt
     7501 gcaattttca tgcaaccaac caaaaaccaa tccagcaatg tatttgatgc cagttcaggt
     7561 gatttgtttg aacgcagtaa agctcgcggt tttgaagctg taatgcagcg tttgacaggg
     7621 attttagtct ttttctggct agccattgcc ttagcattga cggtattatc aagtagataa
     7681 gaaaataatg ggcaggacta ggtctttgcc tctttttatt tttaaaggat gtttgagaag
     7741 gttttacagt aaaagaaaat taaaaaatct agaaagaaaa tatgaaagat agaataaaag
     7801 aatatttaca agacaaggga aaggtgactg ttaatgattt ggctcaggct ttgggaaaag
     7861 acagttccaa ggattttcgt gagttgatta aaaccttgtc cttaatggaa agaaagcacc
     7921 aaattcgttt tgaagaagat ggtagtctga cattagaaat taagaaaaaa catgagatta
     7981 ccctcaaggg gatttttcat gcccataaaa atggctttgg ctttgttagt ctggaaggcg
     8041 aggaggacga cctttttgta gggaaaaatg atgtcaacta tgctattgat ggtgataccg
     8101 tcgaggtagt gattaagaaa gtcgctgacc gcaataaggg aacagcagca gaagccaaaa
     8161 ttattgatat cctagaacac agtttgacaa cagttgtcgg gcaaatcgtt ctggatcagg
     8221 aaaagcctaa gtatgctggc tatattcgtt caaaaaatca gaaaatcagt caaccgattt
     8281 atgttaagaa accagcccta aagttagaag gaacagaagt tctcaaggtc tttatcgata
     8341 aatacccaag caagaaacat gatttctttg tcgcgagtgt tctcgatgtg gtgggacact
     8401 caacggatgt cggaattgat gttcttgagg tcttggaatc aatggacatt gtatccgagt
     8461 ttccagaagc tgttgtcaag gaagcagaaa gtgtgcctga tgctccgtct caaaaggata
     8521 tggaaggtcg tctggatcta agagatgaaa ttacctttac cattgacggt gcggatgcca
     8581 aggacttgga cgatgcagtg catatcaagg ctctgaaaaa tggcaatctg gagcttgggg
     8641 ttcacatcgc agatgtttct tattatgtga ccgaggggtc tgcccttgac aaggaagccc
     8701 ttaaccgtgc gacttctgtt tacgtgacag accgagtggt gccaatgctt ccagaacgac
     8761 tatcaaatgg catctgctct ctcaatcccc aagttgaccg cctgacccag tctgctatta
     8821 tggagattga taaacatggt cgtgtggtca actataccat tacacaaaca gttatcaaga
     8881 ccagttttcg tatgacctat agcgatgtca atgatatcct agctggcgat gaagaaaaga
     8941 gaaaagaata tcataaaatt gtatcaagta tcgaactcat ggccaagctt catgaaactt
     9001 tagaaaacat gcgtgtgaaa cgtggagctc tcaattttga taccaatgaa gcgaagattt
     9061 tagtggataa acaaggtaag cctgttgata tcgttcttcg gcagcgtggc attgccgagc
     9121 ggatgattga gtcttttatg ttgatggcta atgaaacagt tgccgaacat ttcagcaagt
     9181 tggatttgcc ttttatctat cgaattcacg aggagcctaa ggctgaaaag gttcagaagt
     9241 ttattgatta tgcttcgagt tttggcttgc gcatttatgg gactgccagt gagattagtc
     9301 aggaggcact tcaagacatc atgcgtgctg ttgagggaga accttatgca gatgtattgt
     9361 ccatgatgct tcttcgctct atgcagcagg ctcgttattc ggagcacaat cacggccact
     9421 atggactagc tgctgactat tatactcact ttaccagtcc aattcgtcgt tatccagacc
     9481 ttcttgttca ccgtatgatt cgggattacg gccgttctaa ggaaatagca gagcattttg
     9541 aacaagtgat tccagagatt gcgacccagt cttccaaccg tgaacgtcgt gccatagaag
     9601 ctgagcgtga agttgaagcc atgaaaaagg ctgagtatat ggaagaatac gtgggtgaag
     9661 agtatgatgc agttgtatca agtattgtca aattcggtct ctttgtcgaa ttgccaaaca
     9721 cagttgaagg cttgattcac atcactaatc tacctgaatt ttatcatttc aatgagcgtg
     9781 atttgactct tcgtggagaa aaatcaggta tcactttccg agtgggtcag cagatccgta
     9841 tccgtgttga aagagcggat aaaatgactg gagagattga tttttcattc gtacctagtg
     9901 agtttgatgt gattgaaaaa ggcttgaaac agtctagtcg tagtggcaga gggcgtggtt
     9961 caaatcgtcg ttcggataag aaggaagaca agagaaaatc aggacgctca aatgataagc
    10021 gtaagcattc acaaaaagac aagaagaaaa aaggaaagaa acctttttac aaggaagtag
    10081 ctaagaaagg agccaagcat ggcaaagggc gagggaaagg tcgtcgcaca aaataaaaag
    10141 gcacgccacg actatacaat cgtagatacg ctagaggcag ggatggtcct gactggaact
    10201 gaaatcaaga gtgtacgagc tgctcgaatt aatctcaagg atggctttgc tcaagtgaaa
    10261 aatggagaag tttggctgag caatgttcat atcgcgcctt acgaagaggg caatatctgg
    10321 aaccaggaac cagaacgtcg tcgtaaactc ctgctccata aaaagcaaat tcaaaaattg
    10381 gaacaagaga tcaaagggac aggaatgacc ttagttcccc ttaaggtcta tataaaagat
    10441 ggctacgcta agcttctttt aggacttgcc aaagggaagc atgactatga caaacgggag
    10501 tctatcaaac gtcgtgagca aaatcgagat atcgcgcgtg tgatgaaagc tgttaatcag
    10561 cgataaaaag aggaattgaa aatggaaaaa ttagttgcct ataaacgcat gcctttgtgg
    10621 aataaacaaa caatgcctga agctgttcag caaaagcaca atacaaaagt tgggacttgg
    10681 gggaaaatta ctgtcttgaa gggagctctc aagtttattg aattgacaga agaaggggaa
    10741 gttctagctg aacacctctt tgaagcaggg gcagacaatc caatggccca acctcaagcc
    10801 tggcaccgag tggaagctgc cacagatgat gtggaatggt acttggaatt ttattgtaag
    10861 cctgaggatt attttgctaa aaaatacaat accaatcctg ttcattcaga ggtcctagag
    10921 gccatgcaga cagtgaaaca agggaaagct ttggatttgg gttgtggtca ggggcgtaat
    10981 tctctttttc tagcccagca agattttgat gtgacggttg tagatcaaaa tggactggct
    11041 cttgaaatct tgcaaagcat tgtggagcag gaagatttgg acatgcctgt tggcctttac
    11101 gatatcaatt cagctagcat tggacaagaa tatgatttta tcgtttcaac agttgttctc
    11161 atgtttctac aagcggaccg cattccagct attattcaaa atatgcagga gaaaaccagt
    11221 gttggtggtt acaaccttat cgtttgtgcc atggacacgg aggattatcc ttgctcggtt
    11281 aatttcccat tcacctttaa agaaggagaa ctggcagact attacaagga ttgggaattg
    11341 gttaagtaca atgaaaatcc aggccatttg caccgtcgcg atgagaatgg caatcgtatt
    11401 caactacgct ttgcgacctt actagctaag aaaatcaagt aaacacacat gaagattagg
    11461 aattttcctg atcttttttc ttttttacga atgatataga aaaggaggga attcatgttt
    11521 gttgcgagag atgctagggg agaattggta aatgtgttag aggataaact tgagaagcaa
    11581 gcatacacct gcccagcttg tggaggccag atccatttgc gtcaaggacc aagtgtacgg
    11641 acgcattttg cccataaatc cttaaaagac tgtgattttt tctttgaaaa tgaaagtcca
    11701 gaacacctgg ccaataagga atccctctat cactggttga aaaaagagac aaaggttcaa
    11761 ttagagtacc cgctttcaga acttaaacag attgcggatg tatttgtaaa tggcaatcta
    11821 gctctagaag ttcagtgtag tcccttgcct cagaaagtcc ttaaagagcg aagtgagggc
    11881 tatcgtagtc agggttacca agtactgtgg ttgctgggtc aaaaactgtg gctcaaggag
    11941 cgtttgactc gtctacagca aggttttctt tatttcagtc aaaacatggg cttttatgtt
    12001 tgggaattag acaaggaaaa acaagtttta agactcaaat acctgattta ccaggatctc
    12061 cgcggtaaac tccattatca aatcaaggaa ttttcctatg gtcaaggtag tttattggaa
    12121 atattgcgtc ttccctataa gagacaaaaa atatctcatt ttacagtttc tgaggacaag
    12181 gacatctgtc gctatatccg gcaacaactt tattatcaaa atctcttttg gatgaaagaa
    12241 caagcagaag cctatcaaaa gggagaaaat atcctgactt atggactgaa agaatggtat
    12301 ccacaaattc gaccaatagt gggcaaattt ttccagattg aacaagactt gactagctat
    12361 tatcagcact tttataccta ttaccaaaaa aatcctcaaa atgattggca aaagctttat
    12421 ccaccagcct tttatcagca atatttcttg aaaaatatgg tagaatagaa aggatggagg
    12481 aatctaatgg tattacaaag aaatgaaata aatgaaaaag atacatggga tctatcaacg
    12541 atctacccaa ctgaccaggc ttgggaagaa gccttaaaag atttaacaga acaattggag
    12601 acagtagccc agtatgaagg ccatctcttg gatagtgcgg ataacctact agaaatcact
    12661 gaattttctc ttgaaatgga acgccagata gagaagcttt acgtttatgc tcatatgaag
    12721 aatgaccagg atacacgtga agctaagtat caagagtact atgccaaggc catgacattc
    12781 tacagccagt tagaccaagc cttttcattc tatgagcctg aatttatgga gattagcgaa
    12841 aagcagtatg ctgacttttt agaagctcaa ccaaagctgc aggtttatca acactatttt
    12901 gacaagctct tgcaaggcaa ggatcacgtt ctctcacaac gtgaagaaga attattggct
    12961 ggagctggag aaatctttgg ttcagcaagt gaaaccttcg ctatcttgga caatgcggat
    13021 attgtgttcc cttatgtcct agacgatgat ggtaaagaag ttcagctatc tcatgggact
    13081 tacacacgtt tgatggagtc taaaaaacgt gaggttcgcc gtggtgccta tcaagctctt
    13141 tatgcgactt acgaacaatt ccaacacacc tatgccaaaa ccttgcaaac caatgttaag
    13201 gtgcaaaatt accgtgctaa agttcgtaac tacaagagtg ctcgtcatgc agccctcgca
    13261 gcgaattttg ttccagaaag tgtttatgac aatttggtag cagcagttcg caagcatttg
    13321 ccactcttac atcgctatct tgagcttcgt tcaaaaatct tggggatttc agatctcaag
    13381 atgtacgatg tctacacacc gctttcatct gttgaataca gttttaccta ccaagaagcc
    13441 ttgaaaaaag cagaagatgc tttggcagtc ttgggtgagg attacttgag ccgtgttaaa
    13501 cgtgccttca gcgagcgttg gattgatgtt tacgaaaatc aaggcaagcg ttcaggtgcc
    13561 tactctggtg gttcttacga taccaatgcc tttatgcttc tcaactggca agacaatctg
    13621 gacaatctct ttactcttgt tcatgaaaca ggtcacagta tgcattcaag ctatactcgt
    13681 gaaactcagc cttatgttta cggagattac tctatctttt tggctgagat tgcctcaact
    13741 accaatgaaa atatcttgac ggagaaatta ttggaagaag tggaagacga cgcaacacgc
    13801 tttgctattc tcaataactt cctagatggt ttccgtggaa cagttttccg ccaaactcaa
    13861 tttgctgagt ttgaacacgc cattcaccaa gcagatcaaa atggggaggt cttgacaagc
    13921 gatttcctaa ataaactcta cgcagacttg aaccaagagt attatggttt gagtaaggaa
    13981 gacaatcctg aaatccaata cgagtgggct cgcattccac acttctacta taactactat
    14041 gtatatcaat attcaactgg ctttgcggcc gcctcagcct tggctgaaaa aattgtccat
    14101 ggtagtcaag aagaccgtga ccgctatatc gactacctca aggcaggtaa gtcggactat
    14161 ccacttaatg tcatgagaaa agctggtgtt gatatggaga aggaagacta cctcaacgat
    14221 gcctttgcag tctttgaacg ccgtttaaat gagtttgaag cccttgttga aaaattagga
    14281 ttggcataaa atggttgaat cgtatagtaa gaatgctaac cataacatgc gtcgtcctgt
    14341 cgtcaaagaa gaaattgtag acttgatgcg tcagcgtcaa aagcaggtca caggtttctt
    14401 gaaagaattg gaagactttg cccgcaagga aaatattcct attattcccc atgaaacggt
    14461 tgcttatttc cgttttctta tggaaaccat gcagcctaaa aatattctgg aaattgggac
    14521 ggctatcggt ttttcagctc tcttgatggt tgaacatgcg ccaaatgcta agattacaac
    14581 tattgatcgt aatccagaaa tgattggttt tgccaaggaa aattttgccc agtttgacag
    14641 tcgcaagcaa atcactctcc tagagggaga tgcggtggat gtcttatcta cactgacaga
    14701 gtcttatgat ttcgtcttta tggattctgc caagtctaaa tacatcgtct ttctgccaga
    14761 aatcctcaaa catttggaag ttggtggtgt ggttgtcttg gatgatattt ttcaaggtgg
    14821 tgatgttgcc aaggatatta tggaagtccg tcgtggtcag cgaaccattt atcgaggcct
    14881 tcaaaaatta tttgatgcaa ccttagacaa tccagaactc accgcaacat tagtgccttt
    14941 aggagatggt attctcatgc ttcgtaaaaa tgtagcagat gttcaactgt ctgaaagcga
    15001 atgattttca gaaaaattta agaaaaaata gtaaaataga tagagtaaca cttatctcaa
    15061 aggagtagac atgaagaaaa aattattggc aggtgccatc acactattat cagtagcaac
    15121 tttagcagct tgttcgaaag ggtcagaagg tgcagacctt atcagcatga aaggggatgt
    15181 cattacagaa catcaatttt atgagcaagt gaaaagcaac ccttcagccc aacaagtctt
    15241 gttaaatatg accatccaaa aagtttttga aaaacaatat ggctcagagc ttgatgataa
    15301 agaggttgat gatactattg ccgaagaaaa aaaacaatat ggcgaaaact accaacgtgt
    15361 cttgtcacaa gcaggtatga ctcttgaaac acgtaaagct caaattcgta caagtaaatt
    15421 agttgagttg gcagttaaga aggtagcaga agctgaattg acagatgaag cctataagaa
    15481 agcctttgat gagtacactc cagatgtaac ggctcaaatc atccgtctta ataatgaaga
    15541 taaggccaaa gaagttctcg aaaaagccaa ggcagaaggt gctgattttg ctcaattagc
    15601 caaagataat tcaactgatg aaaaaacaaa agaaaatggt ggagaaatta cctttgattc
    15661 tgcttcaaca gaagtacctg agcaagtcaa aaaagccgct ttcgctttag atgtggatgg
    15721 tgtttctgat gtgattacag caactggcac acaagcctac agtagccaat attacattgt
    15781 aaaactcact aagaaaacag aaaaatcatc taatattgat gactacaaag aaaaattaaa
    15841 aactgttatc ttgactcaaa aacaaaatga ttcaacattt gttcaaagca ttatcggaaa
    15901 agaattgcaa gcagccaata tcaaggttaa ggaccaagcc ttccaaaata tctttaccca
    15961 atatatcggt ggtggagatt caagctcaag cagtagtaca tcaaacgaat agtccaaatc
    16021 aatgaatcag ggaaaaaact cgacttcagg aaaaaatgaa gcaaacattc ccacaataaa
    16081 acgcgtagta caaggtttgt actgatcccc aaaagttaga caaataattt atccgaagga
    16141 tttagttctg tattgcacag ggctaagtcc ttttagtttt accttaattc tcttgttgtt
    16201 gtaataatca atatagtcta taatggctcg ttccaattga ttaagtgatt taaatgtttg
    16261 ctcatagcca taaaacattt cggattttaa aatgccaaag aaagattcca tcctaccgtt
    16321 gtcttggctg tttcctttgc gtgacataga tgcttgaatt cccttactct ctaggaagcg
    16381 atgataagaa tcgtgttgat attgccagcc ttggtcacta tggagaatcg tattctcgta
    16441 gtgcttctct ttgaatgcct gttccaacat taacgatcaa tcaatttaat catgtaccta
    16501 agattagaat tgtttatccc aaatttattt gaaagcttct ctaagctata tccttgtttt
    16561 ctaagttcat agatctgaac tttatcatca taagttaatt tcataataaa aacaccccaa
    16621 aagttagatt ttttctgtct aacttttggg gtgtagttca tgtacacctg atatgatgcg
    16681 ttttataatt ttaaagactt tttgaccagc ctcatttttt taacttgata ctcagtgaaa
    16741 agcaaagatt aaactaggaa gctagctgta ggctgctcaa agaacagctt tgaggttgca
    16801 gataaaactt gtgaggtaac caacatatat aatgtgaagc tgacgtggtt tgaatagatt
    16861 ttagaagggt atgagtctgg aagttttaat ggataatgca agattccata gaatgggtaa
    16921 gctagagttc ttatgtgaag agtttgggca taaactttta ccttttcctc cctactcatc
    16981 ttagtataga aaagtgaatc taaaatagta cataactgct tctaaaacat tcttataaat
    17041 tgatttaaat tctcaaatca tattattcag ttcttatttc attttgctct acaatcctgt
    17101 tgagaagaca cgtgttcata tcaaaaaggt attggcaagt tgcaatacct ttttacaagg
    17161 ttcttttgtc ttatttttgt ttcaactgac tatatctcct atggttctag ttcagaaggc
    17221 taggctataa ttatgattga taagaagtat cattccaagt attgagagtg aatgtttcaa
    17281 aatcatgggt ttctataatg gtcaggctgg catttgctag accgccatct ttacgaagaa
    17341 gtggttcttt atagcctagg agagtacgaa gactggcagt aagattggcg ccgtgtccga
    17401 caattagaat acgctcagct ggactatctt ttaatgattt gataaattgg atggtccgct
    17461 gagttgtact atagagggat tcggctccga acattcgagt gtcaaattga gcaagatttg
    17521 aacgaaaagc ctggatttgt tgcgggtaaa tagcttccag ggttgcaatt ttcaaacctt
    17581 ctaacttccc aagttgccat tcacggagat taggaacgat ttctaaagaa cagggggtat
    17641 ggagttgact ttggataatc tcagcagatt tgaccgctcg aggtaaatca cttgaataaa
    17701 tctgatcaaa aggaatttcc ttgagatact ggccaagtcg ttttagggtt tcaatggatt
    17761 caggaagaag aggagaatca ccgctagcac cttgaaaacg accttcttgg ttccagaggg
    17821 tacgaccgtg gcggacaaag tagagtttca ttacttgatg tcctccaaaa tatctacaaa
    17881 gtctgccttt acaaagctag ccaagtcttg tggcgcgacg ataatgctgt gtccgacttc
    17941 gcctgcagag acaatcattc gatccaaatc tagagcaatt ttatcgataa aaatgggata
    18001 attgtgtttc tgacgaattc cgacaggatt attggctcca tgaatgtaac cagttgtttt
    18061 ttctaagtcc ttttgtggaa tcatgctcac ttttttattg ccagaaattt tagctagttt
    18121 cttttcagac aagtgctgag tgatagggac aattccgata atcggtccgg tcttgtctcc
    18181 caaaagcgcc aaggttttga aaatctgatc tcgttcataa ccttgaggaa gctctccttc
    18241 tagggcattg atttgaatcc cctgatgagg gatagctgct ttagatagga tttgttccac
    18301 caatgttttt ttgattttaa ctttttttgc cattatttat atttatcctc caattgactc
    18361 atccaaatac caagccagat tcccagcgca aagaagaagg cgatgatgac ataaccgaca
    18421 agtgaaagtc ctgtgtattg gatactttca gcgtttcctg catttggaat taagatcaaa
    18481 agggtacttg ataggacgat accgatgatg aaatgataga cgcgtgagtg gtagttgttt
    18541 aaggcataat ccatcaattt tgaaaaaacg atgagagttg cacctgcacc aattccaatc
    18601 ggaaagaagg ttcccaagag atcaaaagtt ttaaaaccag tcaacatagg agcatagagt
    18661 cccaaaatca aaagtaaatt cgatgggctg aggccaggaa ccaagacgcc aagggccaat
    18721 agtgcgcctg ctaggacgaa gttaagaaag ctggcgctta aggttccaac gacaaaattt
    18781 aaggcatata gtcctaatcc agaaatgata aaggttgtcc ataaccaagc taaatcaatc
    18841 ttgtctcggt cagattctcg agttgattct ttgaggaggc taggaactgt accgataata
    18901 gctcccgcaa agctccataa tacaaagacc tgataatttt caagcaggta ttcaatcggg
    18961 taggaaaata agccgattcc cagaagcata ccgatggcaa ctggaataaa gtacaaaaca
    19021 ttttctttaa ggtctttaaa gggatgggcc agaaagccaa tcattcgttc atagattcct
    19081 aagattgctg ctagaacccc tccggaaatt cccggtagga taaatccaag agcaattaca
    19141 atccctttaa taacgcgtgc taaccatgag agcatatatt tcctccaact atttctattt
    19201 caaaaaatcc ttactatatt gtatcacaat cagacacaaa aagaaaaagc aaatgataaa
    19261 caaatgcttt taaggtttta aaaaaagctt tcgaaaggtt tcttctttat tttttaaggg
    19321 agagataacg ttgatatcta aatcgtggtc aaagccggca atttttcctt tagatgtgta
    19381 ttggtgaata tcataatcta aatcagtttt aggactgctc tccaaaaatc ctgagtctga
    19441 gccgtaggac ggaatccaaa cagaggtaaa cttgcctgta tcaatactgt gttcttccat
    19501 gaagtagaca ccaacgtaga tgccgatgtt tttagcacct agtgatgcta gttttgctcg
    19561 aaagttttcg acaccttcgt tcatattaga catggttttg tcttccacgt caagccaata
    19621 gtaactaggg ctgtaaggag aggcagcatt gtagaaaact tcggcagcct tttccatttc
    19681 ttgggcactt tttccagcta cataagcgta gacagcaact gggacattcc gcttttgaag
    19741 ttcagtgata tgactcttat aggccttgtc tattccattg ataaatgaag catcattttc
    19801 ttttgttgtt tgagcaccac tgtgaacacg aacaatagca cctgaaatat tttgtgagag
    19861 ggcatcgtag ttgatttcct caggacgctg ccagccagag aggtcaataa tcggtttgtc
    19921 taagtgtttc aaagcctgtg cttcaatctg tgctatattg gattttgttt taaacgattg
    19981 gctgtcatta agtgggcgat tgatgattaa aatgaacatc ataatcccaa aaaaactaaa
    20041 taaaataagt ggatgaattt gttttctcat atcttataag tctaccctaa aaatcaaaaa
    20101 aaatcaaaaa aatgggttaa ggaagagact ttagagcatt ttttcattca agagtgcgga
    20161 atgatttgaa atatggtata ataaaaggga atttctacag aaaagagaag attatgtcaa
    20221 attttgccat tattttagca gcgggtaaag ggactcgcat gaaatctgat ttgccaaaag
    20281 ttttgcacaa ggttgcgggt atttctatgt tggaacatgt tttccgtagt gttggagcta
    20341 tccaacctga aaagacagta acagttgtag gacacaaggc agaattggtt gaggaggtct
    20401 tggctggaca gacagaattt gtgactcaat ctgaacagtt gggaactggt catgcagtta
    20461 tgatgacaga acctatctta gaaggtttgt caggacacac cttggtcatt gcaggagata
    20521 ctcctttaat cactggtgaa agcttgaaaa acttgattga tttccatatc aatcataaaa
    20581 atgtggccac tatcttgact gctgaaacgg ataatccttt tggctatgga cgaattgttc
    20641 gtaatgacaa tgctgaggtt cttcgtattg ttgagcagaa ggatgctaca gattttgaaa
    20701 agcaaatcaa ggaaatcaac actggaacat acgtctttga caacgagcgt ttgtttgagg
    20761 ctttgaaaaa tatcaatacc aataacgctc aaggcgaata ctatattaca gacgtcattg
    20821 gtattttccg tgaaactggt gaaaaagttg gcgcttatac tttgaaagat tttgatgaaa
    20881 gtcttggggt aaatgaccgt gtggcgcttg cgacagctga gtcagttatg cgtcgtcgca
    20941 tcaatcataa acacatggtc aacggtgtta gctttgtcaa tccagaagca acttatatcg
    21001 atattgatgt tgagattgct ccggaagttc aaatcgaagc caatgttatc ttgaaagggc
    21061 aaacgaaaat tggtgctgag actgttttga caaacggtac ttatgtagtg gacagcacta
    21121 tcggagcagg agcggtcatt accaattcta tgattgagga aagtagtgtt gcagacggtg
    21181 tgacagtcgg tccttatgct cacattcgtc caaattcaag tctgggtgcc caagttcata
    21241 ttggtaactt tgttgaggtg aaaggatctt caatcggtga gaataccaag gctggtcatt
    21301 tgacttatat cggaaactgt gaagtgggaa gcaacgttaa tttcggtgct ggaactatta
    21361 cagtcaacta tgacggcaaa aacaaataca agacagtcat tggagacaat gtctttgttg
    21421 gttcaaattc aaccattatt gcaccagtag aacttggtga caattccctc gttggtgctg
    21481 gttcaactat tactaaagac gtgccagcag atgctattgc tattggtcgc ggtcgtcaga
    21541 tcaataaaga cgaatatgca acacgtcttc ctcatcatcc taagaaccag taggagccta
    21601 tcatggagtt tgaagaaaaa acgcttagcc gaaaagaaat ctatcaagga ccaatattta
    21661 aactggtcca agatcaggtt gaattaccag aaggcaaggg aactgcccga cgggatttga
    21721 ttttccacaa tggggctgtc tgtgttttag cagtaacgga tgaacaaaaa cttatcttgg
    21781 tcaagcagta ccgcaaagct atcgaggctg tctcttacga aattccagcc ggaaaattgg
    21841 aagtaggaga aaacacagcc cctgtggcag ctgcccttcg tgaattagag gaagaaacag
    21901 cctatacagg gaaattagaa ctcttgtacg atttttattc agctattggc ttttgtaatg
    21961 agaagttaaa actatattta gcaagcgatt tgacaaaagt ggaaaatccg cgtccgcagg
    22021 atgaggatga aaccttggaa gtccttgaag tgagcttaga agaagcgaaa gaattaatcc
    22081 aatcaggtca tatctgtgat gccaagacaa ttatggctgt tcagtattgg gagttgcaga
    22141 aaaaatagag gaggtcagta tgggtaaatc tttattaacg gatgaaatga ttgaaagagc
    22201 taatagaggc gaaaaaattt caggtcctcc tttgctagat gataatgagg aaactaagat
    22261 tttaccaacc tcttcttccc gttttggtta tgccaatcct aaggatcatg gttttagcca
    22321 ggaaaccttg aagattcagg tcgaaccatc tattcataaa agccgtcgta ttgaaaatac
    22381 caagagaaat gtcttcaatt ctaagttgaa taaaatctta tttgcggtca tctttctctt
    22441 gattttgctt gttttagcaa tgaaactttt gtaatagaaa aggaattgaa atgaaaatag
    22501 gaattattgc tgctatgcca gaagaactgg cttatctggt ccagcattta gataataccc
    22561 aggagcaagt tgttttgggg aatacctatc atacaggaac cattgcttct catgaagtcg
    22621 ttcttgtaga aagtggaatt ggtaaggtca tgtctgctat gagtgtggcg attttggctg
    22681 atcatttcca ggtggatgcc cttattaata cgggttcagc tggggcagta gcagaaggta
    22741 tcgctgttgg ggatgtcgtg attgctgaca aattagccta tcatgacgtg gatgtcacag
    22801 cttttggcta tgcttatgga caaatggcgc aacaaccgct ttatttcgaa tcagacaaaa
    22861 cctttgttgc tcaaatccaa gagagtttat ctcaattgga ccaaaactgg catcttggtt
    22921 tgattgctac aggagatagt tttgttgcag gaaatgacaa gatagaagcg attaagtccc
    22981 atttcccaga agttttagcc gtggagatgg agggggcagc tattgctcaa gcagcgcatg
    23041 ccctcaatct cccagtctta gtcatcagag ctatgagtga caatgccaac catgaagcaa
    23101 acatcttttt tgatgagttt attatcgaag ctggacgtcg ctctgcccaa gtcttgttga
    23161 cctttttgaa ggctttagat taagcggaaa tttgacagtt tttctagctt atgataagat
    23221 ttaagtaaag aaaagctaga aaacgtttca gaggatatta tgagtattga aatgaccgtc
    23281 agtgagattg cagaggtctt aggattatct cgccaagcaa tcaataaccg tgtcaaagaa
    23341 ttaccagaag aagacacaga taaaaatgac aaaggggtaa cagttgttac cagaagtggc
    23401 ttgattaagc tagaagaaat ctataaaaaa acgatttttg aagatgagcc tgtcagtgaa
    23461 gatgtcaaac aacgtgaact gatggagatt ctagtggatg agaagaatgc agaaattctt
    23521 cgtctttatg aacaattaaa agccaaggat cgtcagttat cagaaaaaga cgagcagatg
    23581 cgtatcaaag accgtcagat tgctgagaaa gacaaacaat tagatcagca acaacaattg
    23641 acccttcaag ctatgaagga tcaagaaaat cttaagctag agctggacca agcaaaagaa
    23701 gaagtccaat ccactaagaa aggctttttt gctcgtttat ttggaggata atcaaggagc
    23761 tgtttaggtt aaatgctaac ctgatggctt cttttgtttg tgaatagtat agttgattag
    23821 aacagagaca gtagaggaga ggtacagaat gaaacgatta gaagattaca ttgcttttga
    23881 tttagaattt aatcagcacg aaggacaaac acacttgatt caagtatcgg cagtgcgttt
    23941 tagagatggt cgggaaatcg atgcctatga ttcctatgtt cataccagcg ttcctctaaa
    24001 aagctttatc aatggtttga ccgggattac agctgagacc ttaaaagatg ctcctccagt
    24061 tgagaaagtc ttagaagaat tccaagagtt tgtgggttct ttgctgatga taggctacaa
    24121 tgctgctaaa agtgatttac ctattttagc agagcatggt ttagactata gccaacagta
    24181 caaggtggat ttgtatgatg aagcttttga acgtcggagt tcagatttac atggtattgc
    24241 caatctcaaa ttgcaaactg ttgcctcatt tcttggattt caaggtcagt ctcataatag
    24301 tttagaggat gctcggatga cggcgcgtgt ttacgaatcc ttcttagagt cggatcaagc
    24361 tagggaattg ttagagacag aaagtagctt atcaaacaat ccttttggtg gcttggactt
    24421 gtctcaatta tttgactagg agtgcctatg aaacctgaaa aacctaaaaa attgggtctt
    24481 aaacagattt atctcaatct tgataaaatc ctctttctat ttttcttgat ttttatgata
    24541 gtggagtatt tgtgggtacc attcaattca tggttggcta gcttcttatt agatcagaca
    24601 ggttatcttt ttatttccta taacaatgtc ttagccatcc ttactcattc tccattgatt
    24661 agtctagctt ttttagcctt gattgtaatt aaccttctgg ttgcttattt tcaaatagga
    24721 ctcctcttta ttggtgctcg tcatctcctc tataatgaaa agcgaaggct aattgagtat
    24781 atccgtaagg ttttccatga aagttttgga tttatgaaaa agctaacttg cgctaaagcc
    24841 ttgtttatct ttttctatat agctatgctt tttcctttta tacggaaaat gctgaagatt
    24901 tattatctta ataaaatcgt cattccagag tttatccaag cttatttaga agataaatat
    24961 tggatgtggt ggctgagcat cctacttcta tctcttatct ttctctatgt ctctgtcaga
    25021 ttgatgtttg cccttccgaa gattgtatat gatcagctga ctgttcgaga agcagtgatg
    25081 tttagcttgg agaaaaccaa gaagagagtt actttttatg cttggaattt attttacatc
    25141 ttgctcaaag cacatttatt attttatctt cctttgatcc ctttattatt ggctcaaact
    25201 ttggtagatg atatcactca gagagagtct ctgattcttg gtattttgaa ttttgtcgtg
    25261 attaaaaatc tctattatat ggctctgact tattttttgg ttaaatttgt ttcatttttg
    25321 acaggaaagg agctggatat gcttcctagg agagaaaaag atcacatcgt gcgatggggt
    25381 gtcatgactt gtgccagtct tttctttgcc ttagaaggtt atatttatct ggaggctccc
    25441 atggttcatc tacctcaact tatttctcac cgaggggttt ccaatgcaaa tgggattcaa
    25501 aatacagtag agtccttgga aactacagca caactcaaac cagacttggt ggagacggac
    25561 gtgcaggaaa caaaagatgg gcagtttgtc atgatgcatg atgctaactt gaaaaatcta
    25621 gcaggtatca ataaaagtcc tcaagactta aacttggagg agcttaaagg gattgatatt
    25681 tttgaaaatg gctaccagac taaaatttca agctttgaag attatctcag tcgagccaac
    25741 gaacttggtc aaaaattact aattgaaatt aaaaccagta aaaaagatag tccagacatg
    25801 atgaaccgct ttttagcccg ttatgctgca aagctcaaga tttatggaca tcaaatccag
    25861 tctttagact accatgttgt cgaaaaagta agacagtatg atgccgaact gccagtttat
    25921 ttcatcatgc cctacaattc tgtctttcct aaaacaagag cgacaggata tactatggag
    25981 tactcaacct tggatgaata ttttgtaagc aaactatgga caacggatca gaaactttat
    26041 gtctggactg tcaatgattc agaagctatc agcaaatctc ttcacttagg agtagatgga
    26101 gtgattacag atgatttgga aaaagttcag cgggaaatag aagtagccca agaagatcca
    26161 gaatatacgg atttgctctt gaaaaaagca ttggaattct ttgaatttta gtaataaaaa
    26221 agaggaacgg ttgttcctcc catgataggt aagtattctt gtgaatgctt gcttttttgt
    26281 ggttgcaatc cgtttacgag aattgtttgt attgttcttg tttcaattta ctttatagtg
    26341 ctttgaagtt ggaatgctac actataactt at
//
LOCUS       DAVDWT010000027        26252 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVDWT010000027 DAVDWT010000000
VERSION     DAVDWT010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26252
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.027"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            168..665
                     /locus_tag="VUW50_001447"
     CDS             168..665
                     /locus_tag="VUW50_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7983329.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            665..2320
                     /gene="dnaX"
                     /locus_tag="VUW50_001448"
     CDS             665..2320
                     /gene="dnaX"
                     /locus_tag="VUW50_001448"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET7983330.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2348..2542
                     /locus_tag="VUW50_001449"
     CDS             2348..2542
                     /locus_tag="VUW50_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HET7983331.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2692..3462
                     /gene="sufC"
                     /locus_tag="VUW50_001450"
     CDS             2692..3462
                     /gene="sufC"
                     /locus_tag="VUW50_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET7983332.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3490..4752
                     /gene="sufD"
                     /locus_tag="VUW50_001451"
     CDS             3490..4752
                     /gene="sufD"
                     /locus_tag="VUW50_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET7983333.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIECVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIVGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4763..5989
                     /locus_tag="VUW50_001452"
     CDS             4763..5989
                     /locus_tag="VUW50_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET7983334.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5976..6416
                     /locus_tag="VUW50_001453"
     CDS             5976..6416
                     /locus_tag="VUW50_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET7983335.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6470..7882
                     /gene="sufB"
                     /locus_tag="VUW50_001454"
     CDS             6470..7882
                     /gene="sufB"
                     /locus_tag="VUW50_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET7983336.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8113..9354)
                     /locus_tag="VUW50_001455"
     CDS             complement(8113..9354)
                     /locus_tag="VUW50_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET7983337.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEVSNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKTPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9457..10566
                     /locus_tag="VUW50_001456"
     CDS             9457..10566
                     /locus_tag="VUW50_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7983338.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            11100..11447
                     /gene="ssrA"
                     /locus_tag="VUW50_001457"
     tmRNA           11100..11447
                     /gene="ssrA"
                     /locus_tag="VUW50_001457"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11620..11707)
                     /locus_tag="VUW50_001458"
     tRNA            complement(11620..11707)
                     /locus_tag="VUW50_001458"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11671..11673),aa:Ser,seq:gga)
     gene            11870..12610
                     /locus_tag="VUW50_001459"
     CDS             11870..12610
                     /locus_tag="VUW50_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET7983339.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            12607..13518
                     /gene="pfkB"
                     /locus_tag="VUW50_001460"
     CDS             12607..13518
                     /gene="pfkB"
                     /locus_tag="VUW50_001460"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HET7983340.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTVEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            13515..15467
                     /locus_tag="VUW50_001461"
     CDS             13515..15467
                     /locus_tag="VUW50_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7983341.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSAGQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVFVLVGAIVSGVVYGYLCKPQA"
     gene            15631..17856
                     /locus_tag="VUW50_001462"
     CDS             15631..17856
                     /locus_tag="VUW50_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET7983342.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKTLLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKAQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(17901..18329)
                     /locus_tag="VUW50_001463"
     CDS             complement(17901..18329)
                     /locus_tag="VUW50_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983343.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITAGITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            18467..19609
                     /locus_tag="VUW50_001464"
     CDS             18467..19609
                     /locus_tag="VUW50_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET7983344.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSVQPIEAI
                     SKLLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            19618..20832
                     /gene="thiI"
                     /locus_tag="VUW50_001465"
     CDS             19618..20832
                     /gene="thiI"
                     /locus_tag="VUW50_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HET7983345.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGRAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            20967..21791
                     /locus_tag="VUW50_001466"
     CDS             20967..21791
                     /locus_tag="VUW50_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7983346.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYIECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            21813..22552
                     /locus_tag="VUW50_001467"
                     /pseudo
     CDS             21813..22552
                     /locus_tag="VUW50_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            22565..23731
                     /locus_tag="VUW50_001468"
     CDS             22565..23731
                     /locus_tag="VUW50_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET7983347.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     ATTFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
     gene            24239..25732
                     /locus_tag="VUW50_001469"
     CDS             24239..25732
                     /locus_tag="VUW50_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HET7983348.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            25745..>26252
                     /locus_tag="VUW50_001470"
     CDS             25745..>26252
                     /locus_tag="VUW50_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET7983349.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLRDHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     FQLDELNLL"
BASE COUNT         7834 a         4851 c         5774 g         7793 t
ORIGIN      
        1 tcgcttttca ttatagatct tatgggactt tttttctaca ataaaatagg ctccataata
       61 tctatagggg ggttacccac tacaaatatt atagagccca aaatcctttg tttactaaac
      121 aagggatttt tcttttgtct ctgctccttt tttgatataa tagttctatg ttaaaatcag
      181 aaaaacaatc acgttatcaa atgttaaatg aagaattgtc cttcctattg gaaggcgaaa
      241 ccaatgtttt ggctaatctt tccaacgcca gtgctctcat aaaatcacgt tttcctaata
      301 ccgtatttgc aggcttttat ttgttcgatg gaaaggaatt ggttttaggc cccttccaag
      361 gaggtgtttc ctgcatccgt attgcactag gcaagggtgt ttgtggtgag gcagctcact
      421 ttcaggaaac tgttattgtt ggagatgtga cgacctatct caactatatt tcttgtgata
      481 gtctagctaa aagtgaaatt gtggtgccga tgatgaagaa tggtcagtta cttggagttc
      541 tggatctgga ttcttcagag attgaggatt acgatgctat ggatcgagat tatttggaac
      601 aatttgtcgc tattttgctt gaaaagacaa catgggactt tacgatgttt gaggaaaaat
      661 cttaatgtat caagcacttt atcgaaaata tagaagtcaa aacttctccc agttagttgg
      721 tcaagaagtt gtggctaaga ctcttaaaca agcggtggag caagagaaaa taagtcacgc
      781 ttatcttttt tctggtcctc gtggaacggg aaaaaccagt gttgctaaaa tctttgccaa
      841 ggctatgaac tgtcccaatc aagtgggtgg cgaaccttgc aataactgct atatttgtca
      901 agcagtgacg gacggtagtt tagaagatgt cattgaaatg gatgcagctt ctaataatgg
      961 ggtagatgaa attcgcgaaa ttcgtgataa atctacctat gcgcctagcc ttgctcgtta
     1021 taaggtttat atcatagatg aggttcacat gctgtctaca ggggctttta atgccctcct
     1081 aaagacgctg gaagaaccaa cacagaatgt agtctttatt ttggccacta ctgaattgca
     1141 caagattcct gctactattc tatcccgtgt gcaacgtttt gagtttaaat caattaagac
     1201 acaggatatt aaggaacata ttcactatat cttagaaaaa gaaaatatca gttctgaacc
     1261 agaggctgtg gaaatcattg ccagacgggc ggaaggtgga atgcgggacg ccttgtctat
     1321 tttggatcaa gccctgagtt tgacacaggg aaatgagctg acgactgcta tctctgaaga
     1381 aattactggc accattagcc tatcagcctt ggatgattat gtggcggcct tgtctcaaca
     1441 ggatgttccc aaagctttgt cttgcttgaa tcttcttttt gacaatggta agagcatgac
     1501 tcgttttgtg accgatcttt tgcactattt aagagacttg ttaattgttc aaacaggggg
     1561 agaaaatact catcatagtt cagtctttgt agaaaatttg gcacttcctc aaaaaaatct
     1621 gtttgaaatg attcgcttag caacagtgag tttagcagat attaagtcta gtttgcagcc
     1681 caagatttat gctgaaatga tgaccgtccg tttggcggaa atcaagcccg aaccagctct
     1741 atcaggagcg gttgaaaatg aaattgctac gctgagacag gaagttgccc gtctcaaaca
     1801 agagctttct aatgtaggtg cggttcctaa acaagttgca ccagctccta gtcgaccagc
     1861 tacgggcaaa acagtctatc gtgtcgatcg caataaagtg caatctatct tacaagaggc
     1921 cgtcgaaaat cctgatttag cacgtcaaaa tctaattcgt ttgcagaatg cctggggaga
     1981 ggtaattgaa agtctaggtg ggccggacaa ggctctgcta gttggttctc aaccggttgc
     2041 tgccaatgaa caccatgcta ttcttgcttt tgagtctaac ttcaatgctg gtcaaactat
     2101 gaaacgagac aatctcaata ccatgtttgg taatatcctc agtcaggcgg caggtttttc
     2161 acctgagatt ttagctattt ccatggagga atggaaagaa gttcgcgcag ccttttcagc
     2221 caaagccaaa tcttctcaaa ctgaaaaaga agtagaagaa agcctgattc cagaaggatt
     2281 tgaatttttg gctgataaag tgaaggtaga ggaagactaa agaaagattt catgatacaa
     2341 taagtttatg aataaacaac aatttattat tatggcgctg tttacagctg ctgagaccta
     2401 ttttttcaat gaagcctgga tgactggccg ctatattatg gcagcctttt gggcaatttt
     2461 actctttaga aatttccgag tcagttatgt gatgggcaaa atcgttgatg tcatcgatca
     2521 gcattttaat aggaaagact agccctcagc ttccagacaa aatcaaagcc ttttaggctt
     2581 ttttttgtta tactaggaaa gtatatttat agaatttttg ctctatttct ggggaaatca
     2641 gacgtttttc tagtaagtac tgtaaaagtt ttgaaaaaga aaggaactat catgtcagta
     2701 ttagagatta aagatcttca cgttgagatt gaaggaaaag aaattttaaa aggggttaac
     2761 ctgaccctga aaacaggaga aattgccgct atcatgggac caaatggtac aggtaaatcg
     2821 actctttctg ccgctatcat gggaaatcca aactatgaag taactaaagg tgaagttttg
     2881 tttgatggcg taaacatcct tgagttggaa gtggatgagc gtgcgcgtat gggacttttc
     2941 cttgctatgc aatacccatc agaaatccct ggaattacca atgctgagtt tcttcgtgcc
     3001 gctatgaatg cgggtaaaga agatgatgag aagatttcag ttcgtgagtt tattactaag
     3061 ctagatgaaa aaatggaatt gctcaacatg aaagaagaaa tggcagagcg ttacctcaac
     3121 gaaggcttct ctggtggtga gaaaaaacgc aatgaaattc ttcaactttt gatgttggag
     3181 ccaacatttg ctcttttgga cgagattgac tcaggtcttg atattgacgc tcttaaagtt
     3241 gtgtctaaag gtgtcaatgc catgcgtggt gaaggttttg gtgctatgat catcactcac
     3301 taccaacgtc ttttgaacta tatcacacct gatgtggtac acgtgatgat ggaaggtcgt
     3361 gttgtccttt ctggtggtcc agaattggct gcgcgtttgg aacgtgaagg atacgcaaaa
     3421 ttagctgaag aacttggcta cgactacaag gaagaattgt aattccctcg tatcttttag
     3481 gagaagtaaa tgactagaga aaatattaaa cttttttcag aaatgcacgc tgaaccaagc
     3541 tggttggctg atctccgtca aaaagctttt gacaagattg agactttgga attaccagtt
     3601 attgagtgtg tcaaattcca ccgttggaat ctgggtgatg gaacgattac agaaaatgag
     3661 ccatcagcaa atgttccaga tttcacagct ttagatcatc acttgaagtt ggtgcaagta
     3721 ggaactcaaa ctgttttcga acaaactcca gttgagttag ctgaacaggg tgttgtcttc
     3781 acagactttc actcagcttt agaagaaatt ccagagctga tcgaagaatt cttcatgtca
     3841 tctgttaagt atgatgatga caagttggcg gcttaccaca cagcttactt taacagtggt
     3901 gctgtactct atattccaga taacgtagaa atcacagagc caattgaagg aattttctac
     3961 caagatagcg atagcaatgt gccgtttaac aagcatatta tgattatcgt tggtaaaaat
     4021 tctaagatta gttatctgga gcgtttagag tcacgcggtg aaggaagtga caaagcaact
     4081 gccaatatca cagtggaagt gattgcacgt tctggtgcgc aagtcaagtt tgctgctatc
     4141 gaccgtctag gtgaaaacgt cactgcctac attagccgtc gtggtaaatt aggcaacgat
     4201 gcaagtattg actgggctat cggtgtcatg aacgaaggaa atgtcgttgc tgattttgat
     4261 agtgacttga ttggtaatgg tagccatgct gacctcaagg ttgtagctct ttcaagtggt
     4321 cgtcaggtac aagggattga tactcgtgta actaactatg gctgcaactc aatcggaaac
     4381 attctacaac atggggttat ccttgaaaaa gcaactttga ctttcaatgg tatcggccac
     4441 atcatcaagg gtgctaaggg agcagatgcg caacaagaga gccgtgttct catgctttca
     4501 gaccaagcgc gttcagatgc taacccaatt cttttgattg atgaaaatga cgtaactgca
     4561 ggccatgcag cctctattgg tcaggtagat ccagaagata tgtactacct catgagtcgt
     4621 ggcttggata aggcaactgc agagcgtttg gttgttcgtg gtttccttgg atctgttatc
     4681 gtggagattc cagtcaagga agttcgtgat gaaatgattg caactatcga agagaaattg
     4741 tcaaaacgct aaggggcagc ctatgttaga tgtagaagcg attcgcaagg attttccaat
     4801 tttagatcag attgtcaatg atgaacctct ggtctatctg gacaatgctg cgacgacaca
     4861 aaaaccacta gtagttctga aagctattaa cagctactat gagcaggaca atgccaatgt
     4921 tcaccgtggt gtccatacct tagcggaacg agcgacagct tcttatgaag ctgctcgtga
     4981 aaccattcgt aagtttatta atgcaggctc tacaaaggaa gttctcttta ccagaggaac
     5041 gacaaccagc cttaactggg tggcacgctt tgctgaggaa attctcactg agggagacca
     5101 ggtcttgatt tcagtaatgg aacaccattc taatatcatt ccatggcagg aagcttgtcg
     5161 aaagactgga gcagagcttg tctatgtcta tcttaaagac ggtgccttgg atatggagga
     5221 tttgcgagct aaattgactg ataaggttaa atttgtttcc ctagctcatg cctccaatgt
     5281 tcttggtgtg gtcaatccga tcaaggaaat cactcaatta gcccaccaag ttggggcaat
     5341 tatggtagtg gatggtgctc aatctacacc tcatatgaag attgatgtcc aggacttgga
     5401 tctggacttt ttcgcctttt cgggtcacaa gatggctggt ccgactggta tcggtgtcct
     5461 ttacggcaaa gaaaagtatc ttgagcaaat gtctccagta gaatttggcg gcgagatgat
     5521 tgattttgtc tacgagcaat ttgctagttg gaaggaattg ccttggaaat ttgaggctgg
     5581 aacgccaaat atggcaggag ctattggact tgcgactgca gttgattatc tggaaaagat
     5641 tggtatggat gccgttgaag ctcatgaaca ggaattgatt gcgtacgtct atccaaaact
     5701 gcaggcaatt gagggattga ccatttacgg ttctcaggat ttggctcaac gttcgggtgt
     5761 tattgccttt aacctaggtg atctccatcc tcacgatctt gcgacggctc tggattatga
     5821 aggagtggct gttcgtgctg gtcaccattg tgcgcaaccc ttgcttcagt atttggaagt
     5881 cccagcaaca gctcgtgcaa gtttttatat ctacaatacc aaggcagatt gcgacaaact
     5941 agtcgatgcc ctacaaaaga caaaggagtt tttcaatggc actttctaaa ctagatagcc
     6001 tttatatggc agtggtagca gaccattcga aaaatccaca tcaccaaggg aagttagaag
     6061 atgctgagca aatcagtctc aacaatccga cttgtgggga tgtcatcaac ctctctgtca
     6121 agtttgatgc agaggaccgt ttggaagata ttgcttttct aaattcagga tgcacgattt
     6181 caactgcttc tgctagtatg atgacagatg ccgttttagg aaaaaccaaa caagaaattt
     6241 tagaactggc gactattttt tctgaaatgg ttcaagggca aaaagatgag cgtcaagacc
     6301 aacttggaga cgcggcattc ttgtcaggtg ttgccaaatt ccctcaaaga atcaagtgtg
     6361 caaccctagc ttggaatgcc cttaagaaaa caattgaaaa tcaagaaaaa cagtaagaca
     6421 agtttctttt gtcttatgaa ttattagaaa tgaagaaaga aaggatacta tggctgaaga
     6481 aagagtagaa ccaaaaccaa ttgaccttgg tgaatataaa tttggtttcc atgacgatgt
     6541 agagcctgtc ttatcgacag gaaaaggact caacgaaggt gttattcgtg aattatctgc
     6601 tgctaagggt gagcctgagt ggatgttgga gttccgtttg aagtcttatg aaaccttcaa
     6661 aaaaatgccc atgcaaactt ggggagcaga cttgtcagag attgactttg atgacttaat
     6721 ctactaccaa aaaccatctg acaaaccagc ccgttcttgg gatgatgtac ctgaaaagat
     6781 taaagaaacc tttgaacgta tcgggattcc agaagctgaa cgtgcttatt tagcaggggc
     6841 ttctgcccag tacgagtcag aagtggttta ccacaacatg aaggaagagt tccaaaaatt
     6901 aggtattatc tttacagata cagattccgc actcaaggaa tacccagact tatttaaaca
     6961 atactttgcg aagttggtac cgccgacaga taacaagttg gcagccctca actcagcagt
     7021 atggtcgggt ggaactttta tctacgtgcc aaaaggtgtc aaggtagata ttccacttca
     7081 aacttatttc cgtatcaata acgaaaatat aggtcagttc gaacgtacct tgattatcgt
     7141 tgatgaggga gcaagcgtcc actacgtaga aggatgtaca gcaccaacat attcaagcaa
     7201 tagcttacac gctgccattg tagaaatttt tgctttggac ggagcttata tgcgttatac
     7261 aactatccaa aactggtctg ataacgtcta taacttggta acaaagcgtg ctaaggctca
     7321 aaaggatgcc actgttgagt ggattgatgg aaacttgggt gccaaaacga ctatgaaata
     7381 tccatctgtt taccttgatg gagaaggagc gcgtggtacc atgctctcta tcgcctttgc
     7441 taatgcaggg caacaccaag acacgggtgc taagatgatt cacaatgctc cacataccag
     7501 ctcgtctatt gtgtctaaat ccatcgctaa aggtggagga aaggttgact accgtggaca
     7561 agtcaccttt aacaagaact ctaagaaatc tgtttcccac attgaatgtg ataccattat
     7621 catggatgac ttgtcagcat cagatactat tccatttaat gaaattcaca actcgcaagt
     7681 ggctttggaa cacgaagcca aagtatctaa gatttcagaa gagcaattgt attatctcat
     7741 gagccgtgga ttgtcagaat ctgaggcaac tgaaatgatt gtcatgggat ttgtagaacc
     7801 ctttacaaaa gaacttccaa tggaatacgc agttgagctg aaccgcttga ttagctatga
     7861 aatggaggga tcagttggat aaaatttgat tttatactct tcgaaaatct cttcaaacca
     7921 cgtcagcatc gccttaccgt atgtatggtt actgacttcg tcagtttcat ctacaacctc
     7981 aaaacagtgt tttgagcaag ctgcggctag cttcctagtt tgttctttga ttttgagtat
     8041 tagatttact caaaatcaag gattttgaag atgaacttgt atcaaaaaat cgcggtttaa
     8101 aatcgcgatt ttttataatt tctcgttaac aaagcggaca aactgattcc accaaacttt
     8161 taagaagaag gctttttcaa ttttcttgtc tgctaccatt tcgaaactag ggcgctctgt
     8221 ggtgatgtaa ccttgaccaa tcaagtcctt gtcttcataa gtcaaatggc caaccactgt
     8281 tccagcttca agtggtgctg ggattgcttt ggaatcaggt gtgaattgaa cagattggga
     8341 agattgattc ccaacacgtt cgattagata gatatcctct ggagccactg cagttactgt
     8401 atcttctttt ccatcttgta caggggtttt gctatcttga taggcatcgc cttgttgaac
     8461 gattttgcga agtgtaaatg tagaagaaat ataatccatt agggaagatg tagctgtaaa
     8521 tcgagcgtaa ggattattgt cttgatgatc tgcatttaaa acaactgtga tgactctcat
     8581 gcctttttcg acagtagtac caacaaaaga ctctccagcc ttatctgttg ttcctgtttt
     8641 tagcccatca aaaccaccac ggtaagcagg cataccttct aacatgtagt tggttgaagt
     8701 gattgtcatc ccagcaaaag tagaagaagg ttttttggtg atttctaaga cttgtgggta
     8761 ttttttgatg aggttgcgag caacgatagc gacatcataa gcactaagct tattttcctc
     8821 atctttttta gaacctgggt aaatgttatc ccctagagtt tcattgttaa gacctgtcgt
     8881 attgacaaca gtggcatcct gaattcccca ttccaagagt tttgcccgca tcatatcgac
     8941 gaaatctttt tctgagccag caattttctc agctagggca atagcggcgc tgttggcact
     9001 agataccaga gttgcttcaa gcaactcttc gacagtataa ttacgggcct ccataggaat
     9061 attactgact tcagaatttg tcgtcaattg ataaggataa tcagaaatat ctacaggagt
     9121 ggagagggta atacttccgt tttccaaagc ttcatagacc agataaacag taatcaattt
     9181 tgttatggaa gcaatttcga caggttgcgt tgcatccttc tcatagagaa ttttaccagt
     9241 atttgcctca acagcaatcg catgtttagc ggcaatggta aaatcttgag caacagcagt
     9301 agaagcaccc cctaaaagag agacagttaa caaagttaaa aatatttttt tcatagtagt
     9361 cttattctat cataaagaaa aaaaatattc ttgctttaat aattcatctg ttaagctttt
     9421 tgaaaatatg gtaaaataaa gtgagggagg taactcatgt ttcgtagaaa taaattattt
     9481 ttttggacca cagaaatttt actcttaacc atcatctttt acctatggag acagatggga
     9541 tctttgatta acccttttgt tagcgtgctt aatacaatta tgattccatt tttattaggg
     9601 ggctttcttt attatttgac aaaccctatt gttactttct taaataaagt ctgtaaactc
     9661 aatcgtttgc ttggtatttt aattaccttg tgtactttgg tctggggaat ggtcataggt
     9721 gttgtctatc tcttacctat tttgattaat cagttatcta gtttgattat atctagtcaa
     9781 actatttata gtcgagtaca agacttaatc atagacttat ctaattatcc tgcgctccag
     9841 aatttggatg tagaagctac aattcagcag ttaaacttat cctatgttga tattcttcaa
     9901 aatatcctaa atagcgtatc aaatagtgtg gggagcgtct tgtcagctct tatcagtact
     9961 gttttgattt tgattatgac tccagttttt ttggtttatt tcttattaga tggacataaa
    10021 ttcttgccca tgcttgaaag aacgattcta aagagggatc gcttgcatat tgcaggctta
    10081 ttaaagaatt taaatgcgac gattgctcgc tatattagtg gagtttcgat tgacgcaatc
    10141 attataggtt gtttggctta tattggctat agtattattg gtttaaaata tgctttagtt
    10201 tttgccattt tttctggtgt agccaattta attccttatg tggggccaag tattggtttg
    10261 attcctatga tcatcgcaaa tatattcact gatccccata gactgctgat tgcagtgatt
    10321 tatatgcttg ttgttcagca ggtagatggc aatatcttat atcctcgaat cgtaggaagt
    10381 gttatgaagg ttcatccaat cacgatttta gttttacttt tgttgtcaag caatatctat
    10441 ggtgtagttg gaatgattgt cgcagtgcca acctattcta tcttgaaaga aatttctaag
    10501 ttcttatccc gtttgtatga aaatcataaa ataatgaaag aacgagaaag agaattagct
    10561 aagtaaaagt caggagaacc ctgatttttc tttactggaa gtggccttta gattagaaga
    10621 ctgaaaataa gttaaagtct taaactaatt ttcacagcta agaatagtag aagttaatct
    10681 gataaaaatc gaaaaaacca gtggaattct gtgtcagggt aagttccact ggttttcata
    10741 gtctattaaa gttcgaatga aacctattta taatagattg aaactagaat agtacacctc
    10801 taattctaaa acattgttag aaatcgattt gactgtcctt atctattcgt tctattctta
    10861 tttcatttta ctatattttg gtgcaataag tgaaaagtag accgaataat ataaggattg
    10921 attttatagt ttttaaactc aaatgaattg aaataaagag agtacgaaaa ttctcatctg
    10981 aaagtatttt agaataattc tcttcgtgaa tttcttcaaa acagatagct tcatcttagg
    11041 tatgtgattt ctttttgcat ttttgagtta gataaggtat aatggtttta ttgtcttttg
    11101 gggtcgttac ggattcgaca ggcattatga ggcatatttt gcgactcgtg tggcgacgta
    11161 aacgctcagt taaatataac tgcaaaaaat aacacttctt acgctctagc tgcctaaaaa
    11221 ccagcaggcg tgacccgatt tggattgctc gtgttcaatg acaggtctta ttattagcga
    11281 gatacgatta agccttgtct agcggtttga taagagattg atagactcgc agtttctaga
    11341 cttgagttat gtgtcgaggg gctgttaaaa taatacataa cctatggttg tagacaaata
    11401 tgttggcagg tgtttggacg tgggttcgac tcccaccggc tccattattc ctttgcattc
    11461 cttggtaaaa cgttgttaaa tcaacgtttt ttatttttat ctttggtatt cctttgcatt
    11521 cttttgctaa aaagggagtc acaaacagac cctattttaa aaaaaggata gaaaaaagga
    11581 tacaacattt gtcgcatcct aaaaataatc ttttttcgac ggaagacatg ggattcgaac
    11641 ccacgcacgc tattacacgc ctaccgcgtt tccaacacgg cctcttaagc ctcttgagta
    11701 atcttccaat acttactcaa atagtctacc ataaaggctc ttatcttgca ataaaaattc
    11761 tagaaataag aaaaaatgat agattttgaa agaaaatgat aaaaaatgct tgacttcgaa
    11821 agaaagtatg atagaatgaa tagtgtaaac gataacagga ggtgattcag tgttaaaaac
    11881 agaacgtaaa caactaattt tagaggagtt aaatcagcat catgtagttt ctctagaaaa
    11941 attagttagt ttgctagaaa cgtcagaatc aacggttcga agagacttgg atgagttgga
    12001 agcggaaaac aagcttcgtc gtgtgcatgg tggagcagaa ctcccccact ccttacagga
    12061 agaagaaacc attcaagaaa aatctgtcaa aaaccttcaa gaaaagaagt tgctggctca
    12121 gaaagcagcc tctctcatta aagaaaaaga tgtcatcttt atcgatgctg gaacaacaac
    12181 tgcttttttg attcatgaat tggtcaataa gaatgttaca gttgtgacca actccattca
    12241 ccatgccgct cagttggttg aaaagcagat tccaactgtc atggttggag gaaacgtcaa
    12301 gacggcgaca gatgctagta tcgggggcgt tgctcttaac cagattaacc aattgcactt
    12361 tgaccgtgcc tttatcggaa tgaatggtgt tgacgatggc tattatacga ctcctgatat
    12421 ggaggaggga gctgtgaaaa gagctatttt ggagaatgcc aagcagacct acgtcttggt
    12481 ggattcgtca aaaattggac aaacttgctt tgccaaggta gcaccactca aacgcgctat
    12541 cgttatcact agtcaagggc atgagctctt gcaggttatt aaggagaaaa cggaggtaat
    12601 agaagtatga tttatacagt cacactcaat ccatccattg actatatcgt tcgtttggac
    12661 caagtcaaag ttggtagtgt caatcgtatg gacagtgatg ataagtttgc tggtgggaaa
    12721 ggaatcaatg tcagccgtgt cttgaaacgt ttgaatatac caaatacagc gacgggattt
    12781 atcggtggct ttactggtaa atttatcaca gatactctag cagaggaaga aatcgagaca
    12841 cgttttgtcc aagtggcaga agatactcgt atcaatgtta aaatcaaagc agaccaagaa
    12901 acagaaatca acggaacggg tccaactgtt gaaccggttc agctagaaga attgaaagct
    12961 attttatcta gtctgacagt agaagataca gttgtctttg caggttcaag tgctaaaaat
    13021 ctaggcaatg ttatctataa ggatttgatt tccttgacgc gccagactgg tgcgcaagtg
    13081 gtctgtgact ttgaaggaca gaccttaatt gatagtttgg actaccagcc tcttcttgta
    13141 aaaccaaaca atcatgaact tggagcgatt tttggggtta aactcgaaag tttagatgaa
    13201 attgagaaat acgctcgtga gttactggct aagggtgctc aaaatgttat tatctctatg
    13261 gctggtgatg gcgcccttct tgtcacatct gagggagctt acttcgctaa accaatcaaa
    13321 ggaacagtca aaaattcagt tggagctggt gattctatgg ttgctggatt cacaggtgaa
    13381 tttgtcaaat caaaagacgc agtagaagcc ttcaaatggg gagtggcttg cggaacggca
    13441 actaccttct cagatgactt ggcaacggcg gaatttatta aagaaacata tggaaaagtt
    13501 gaggtagaaa aaagatgaaa attcaagacc tattgagaaa agatgtcatg ttgctagatt
    13561 tgcaggcaac tgaaaaaaca gctgtcatcg acgagatgat taaaaatttg acagagcacg
    13621 gttatgtaac agattttgaa acatttaaag aaggaatttt ggcgcgtgaa gctttgactt
    13681 ctactggttt gggtgatgga atcgcaatgc ctcacagcaa aaacgctgct gtcaaagaag
    13741 cgacagttct atttgctaag tcaaacaagg gtgttgacta cgagagcttg gatggacaag
    13801 caactgacct cttcttcatg attgcagctc cagaaggtgc caatgatact cacttggcag
    13861 ccttggcaga attgtctcaa tacttgatga aagacggttt tgcagacaaa cttcgtcaag
    13921 caacatctgc aggccaagtt atcgaacttt ttgaccaagc ttcagaaaaa actgaggaac
    13981 ttgttcaagc acctgctaat gactctggtg actttatcgt agctgttaca gcttgtacaa
    14041 caggtattgc ccacacttac atggcccaag aagcccttca aaaagtagct gctgaaatgg
    14101 gggttggtat caaggtcgaa accaacggtg ctagcggtgt tggaaatcaa ctaactgcag
    14161 aagatatccg taaggctaaa gctattatca ttgcagcaga caaggccgtt gaaatggatc
    14221 gatttgatgg aaaaccattg atcaatcgtc cagttgctga cggtatccgt aagacagaag
    14281 agctaattaa cttggctctt tcaggagata ctgaagtcta ccgtgccgct aatggtgcca
    14341 aagctgcaac agcctctaac gaaaaacaaa gccttggtgg tgccttgtac aaacacttga
    14401 tgagtggtgt atctcaaatg ttaccattcg ttatcggtgg tggtatcatg attgcccttg
    14461 ccttcttgat tgacggtgct ttgggtgttc caaatgaaaa ccttggcaat cttggttctt
    14521 accatgagtt agcttctatg tttatgaaaa ttggtggagc tgcctttggt ttgatgcttc
    14581 cagtctttgc gggttatgtt gcctactcta ttgctgaaaa accgggtttg gtagcaggtt
    14641 tcgtggctgg tgctattgcc aaagaaggtt ttgcctttgg taaaattcct tatgccgcag
    14701 gtggtgaagc aacttcaact cttgcaggtg tctcatctgg tttcctaggt gcccttgttg
    14761 gtggatttat cgcaggtgcc ttggttcttg ccatcaagaa atacgttaaa gttcctcgtt
    14821 cactcgaagg tgctaaatca atccttctat tgccacttct tggaacaatc ttgacaggat
    14881 ttgttatgct agctgtgaat atcccaatgg ctgcaatcaa cactgctatg aatgacttcc
    14941 taggcggtct tggaggaggt tcagctgtcc ttcttggtat cgtccttggt ggaatgatgg
    15001 ctgttgacat gggtggacca gttaataaag cagcttatgt ctttggtaca ggtacgcttg
    15061 cagcaactgt ttcttcaggt ggttctgtag ccatggcagc agttatggct ggaggaatgg
    15121 tgccaccact tgcaatcttt gtcgcaactc ttcttttcaa agataaattt actaaggaag
    15181 aacgtaactc tggtttgaca aacatcatca tgggcttgtc atttatcact gagggagcga
    15241 ttccatttgg tgccgctgac ccagctcgtg cgattccaag cttcatcctt ggttcagcag
    15301 tagcaggtgg actcgttggt cttactggta tcaaactcat ggcgccacac ggaggaatct
    15361 tcgttatcgc ccttacttca aatgctctcc tttacctcgt ttttgtcttg gtaggagcaa
    15421 tcgtaagtgg tgtggtttat ggttacctat gcaaaccaca agcataaaaa atagaaaaat
    15481 gaaaagattg gaccgtttgg tgcagtcttt ttctcttccc gaaatgcctg tgaaatatgg
    15541 tataatagaa gaatggcaaa caagaataca agtacaacaa gacggagacc gtctaaagca
    15601 gaactggaaa gaaaagaagc gattcaacga atgttgattt cgttaggaat tgcgatttta
    15661 ttgattttcg cagccttcaa attaggggct gcaggtataa ccctttataa tttaattcgc
    15721 ttgctagtgg gtagcctagc ttatctggcg atattcggcc tattaatcta tctcttcttt
    15781 ttcaagtgga tacgaaaaca ggaaggactc ttatctggct ttttcaccat atttgctggc
    15841 ttactcttga tttttgaggc ctacttggtt tggaaatatg gtttggacaa gtccgttcta
    15901 aaagggacca tggctcaggt tgtgacagat ctgactggtt ttcgaacgac tagctttgct
    15961 ggagggggct tgatcggggt cgctctttat attccaacag cctttctctt ttcaaatatc
    16021 ggaacttact ttattggttc tatcttgatt ttagtgggtt ctctcctagt cagcccttgg
    16081 tctgtttacg atattgctga atttttcagt agaggctttg ccaaatggtg ggaagggcac
    16141 gagcgtcgaa aagaggaacg ctttgtcaaa caagaagaaa aagctcgcca aaaggctgag
    16201 aaagaggcta gattagaaca agaagagact gaaaaaacct tactcgattt gcctcctgtt
    16261 gatatggaaa cgggtgaaat tctgacagag gaagctgttc aaaatcttcc acctattcca
    16321 gaagaaaagt gggtggaacc agaaatcatc ctgcctcaag ctgaacttaa attccctgaa
    16381 caggaagatg actcagatga cgaagatgtt caggtcgatt tttcagccaa agaagccctt
    16441 gaatacaaac ttccaagctt acaactcttt gcaccagata aaccaaaaga tcaatctaaa
    16501 gagaagaaaa ttgtcagaga aaatatcaaa atcttagaag caacctttgc tagctttggt
    16561 attaaggtaa cagttgaacg ggccgaaatt gggccatcag tgaccaagta tgaagtcaag
    16621 ccggctgttg gtgtaagggt caaccgcatt tccaatctat cagatgacct cgctctagcc
    16681 ttggctgcca aagatgtccg gattgaagca ccaatccctg ggaaatccct aatcggaatt
    16741 gaagtgccca actccgatat tgccactgta tctttccgag aactatggga acaatcgcaa
    16801 acgaaagcag aaaatttctt ggaaattcct ttagggaagg ctgttaatgg aaccgcaaga
    16861 gcttttgacc tttctaaaat gccccacttg ctagttgcag gttcaacggg ttcagggaag
    16921 tcagtagcag ttaacggcat tattgctagc attctcatga aggcgagacc agatcaagtt
    16981 aaatttatga tggtcgatcc caagatggtt gagttatctg tttacaatga tattccccac
    17041 ctcttgattc cagtcgtgac caatccacgt aaagccagca aggctctgca aaaggttgtg
    17101 gatgaaatgg aaaaccgtta tgaactcttt gccaaggtgg gagttcggaa tattgcaggt
    17161 tttaatgcca aggtagaaga gttcaattcc cagtctgagt acaagcaaat tccgctacca
    17221 ttcattgtcg tgattgtgga tgagttggct gacctcatga tggtggccag caaggaagtg
    17281 gaagatgcta tcatccgtct tgggcagaag gcgcgtgctg caggtatcca catgattctt
    17341 gcaactcagc gtccatctgt tgatgtcatc tctggtttga ttaaggccaa tgttccatct
    17401 cgtgtagcat ttgcggtttc atcaggaaca gactcccgta cgattttgga tgaaaatgga
    17461 gcagaaaaac ttcttggtcg aggagacatg ctctttaaac cgattgatga aaatcatcca
    17521 gttcgtctcc aaggctcctt tatctcggat gacgatgttg agcgcattgt gaacttcatc
    17581 aaggctcagg cagatgcaga ctacgatgag agttttgatc caggtgaggt ttctgaaaat
    17641 gaaggagaat tttcggatgg agatgctggt ggtgatccgc tttttgaaga agctaagtct
    17701 ttggttatcg aaacacagaa agccagtgcg tctatgattc agcgtcgttt atcagttgga
    17761 tttaaccgtg cgacccgtct catggaagaa ctggagatag caggtgtcat cggtccagct
    17821 gaaggtacca aacctcgaaa agtgttacaa caataaaaaa atagcttctt tccgagtttg
    17881 gagggaagct attttagtgg ctattgattg cttttatttt ctgaagttgg cgcattggac
    17941 tgtttttcgt tttcagtagc aggtttactt gaagcaggag tagaagagtc ctgagttgct
    18001 gttttctgat cttctttttt ctcttccttg acgctagatt ttggtgtttc ctcttgctgt
    18061 gttttttctt gactagtgtt agtctcttta gttggactgg tgttttcctt aggggattcc
    18121 ttttggattt ctttgacaat ggttgtcgtc tggcttgtcg taggttcttt tttaatattt
    18181 ttgttattat ccaaggcgtt catgatcgtg atacccgcgg taatcaagcc aaggatacta
    18241 gcgattgtag caacggtttt ttgaaagact gtaaagcctt ttttgatgtg ttctgttttt
    18301 ggttttgaag ttctacgata gttagacata cattctctcc tttgattatg atttattata
    18361 ctctgtttct ttaaaaaaat cttaaaaaaa gagaaattgc cctttcttgt cgcaggctcc
    18421 gtttcatgat acaatagaag gaatgatttt agaaagcgtg agaaacatga tttactttga
    18481 taattcggcg acgaccaggc cctatccaga agcccttgaa acctatatgc aggtggcttc
    18541 aaaaatttta ggaaatccat ctagtctcca tcgtttggga gaccaggcaa cacgaatttt
    18601 agatgcttcc cgtcaacaga ttgcagattt aatcggtaag aaaagtgatg aaatcttctt
    18661 tacatctggt gggacagaag gggataactg gcttatcaag ggtgtggcct ttgaaaaagc
    18721 ccagtttggc aagcacatca tcgtatcagc cattgaacat ccagcagtta aggaatcagc
    18781 cctctggttg aaagctcaag gttttgaagt ggattttgct ccagttgata acaaaggatt
    18841 tgtggatgtt gaggcgctag aagatttgat aagacctgat acgaccctcg tttccatcat
    18901 ggcagttaac aatgaaattg gctctgttca gcccattgag gctatttcaa aactattggc
    18961 agacaagcca actatttcct tccacgttga tgcggttcag gcgcttgcca agattccgac
    19021 tgaaaagtat ctgacagaac gagtggattt cgcgactttc tctggtcaca agttccacgg
    19081 aatccgtggt gttggtttta tctatatcaa gtctggcaag aagattacgc ctcttttaac
    19141 aggtggtggt caggaacgtg attaccgttc gacaactgaa aatgtggcag gaattgcagc
    19201 gaccgctaag gctctccgtt tatctatgga aaagctagat atctttagga gcaagactgg
    19261 gcagatgaag gcagtgattc gtcaagcact tctggactat ccggatatat ttgtcttttc
    19321 agatgaggaa gacttcgccc ctcatattct gacttttgga atcaagggtg ttcgtggtga
    19381 ggtcattgtt cacgcctttg aagactatga tattttcatc tcaacgacct cggcttgttc
    19441 gtccaaggct gggaaaccag ccggaaccct gattgctatg ggagtggaca aggataaggc
    19501 tcagtcagct gtgcgtctta gtctagacct tgaaaatgat atgagtcagg tcgagcagtt
    19561 cttgaccaag ctaaaattaa tttacaatca aactagaaaa gtaagatagg agcattcatg
    19621 cagtattcag aaattatgat tcgctacggt gagctgtcaa ccaagggcaa aaatcgtatg
    19681 cgtttcatca ataaacttcg caataatatt tcagacgttt tgtctatcta tccccaagtt
    19741 aaggtaacag cagatcgcga ccgtgcccac gcttacctca atggagcaga ttacacagca
    19801 gtagcagaat cgctcaaaca agtatttgga attcaaaact tttcccctgt ttataaggtt
    19861 gaaaaatctg tagaagttct gaagtctgct gtccaagaga ttatgcagga catctacaag
    19921 gaaggcatga cctttaaaat ctctagcaag cgtagcgacc acacctttga attggacagt
    19981 cgtgaactca accaaacgct tggaagggct gtattcgaag ccattccaaa cgtacaagct
    20041 caaatgaaaa gtcctgacat caatcttcag gtggagatac gtgaagaagc agcctatctt
    20101 tcttatgaaa ccgttcgagg ggcaggtgga ttaccagtgg gaacttctgg taagggcatg
    20161 ctcatgttgt cagggggaat cgattcccct gtagctggct atctagcgct taaacgaggg
    20221 gtagatatag aggcagttca ctttgccagc ccaccttaca cgagtcctgg tgctcttaaa
    20281 aaagcccaag atttgacccg taaattaacc aaatttgggg gaaatatcca gtttatcgaa
    20341 gttcctttta cagagattca agaggaaatc aaagccaaag cgccagaagc ctaccttatg
    20401 accttgacgc gtcgttttat gatgcgtatt actgaccgta ttcgtgaggt acgaaatggt
    20461 ttagttatca tcaatgggga aagtctaggt caagtagcca gccaaaccct tgaaagtatg
    20521 aaggctatca atgctgttac caacactccc atcattcgtc ctgtggttac catggacaag
    20581 ttggaaatca ttgacatcgc ccaggaaatc gatacctttg acatttcaat ccaaccgttt
    20641 gaagactgtt gtaccatttt tgcaccagat cgtccaaaaa caaatcctaa aattaagaat
    20701 gcggagcagt acgaagcgcg tatggatgtt gaaggcttgg ttgagcgagc agtggctgga
    20761 atcatgatta ctgaaatcac acctcaagcc gaaaaagatg aagttgatga cttgattgac
    20821 aatctgctct aattcagaaa atccaaaaga atagcgaaaa tcagtaaaaa aagttagttt
    20881 tttctctaaa aacaggtaaa aaactaactt tttttatttt tatgatataa tgatataaaa
    20941 ttttgaatat agagagtttt ctggcaatga atcaatccta cttttatcta aaaatgaaag
    21001 aacacaaact caaggttcct tatacaggta aggagcgccg tgtacgtatt cttcttccta
    21061 aagattatga gaaagataca gaccgttcct atcctgttgt atactttcat gacgggcaaa
    21121 atgtttttaa tagcaaagag tctttcattg gacattcatg gaagattatc ccagctatca
    21181 aacgaaatcc ggatatcagt cgcatgattg tcgttgctat tgacaatgat ggtatggggc
    21241 ggatgaatga gtatgcggct tggaagttcc aagaatctcc tatcccagag cagcagtttg
    21301 gtggtaaggg tgtggagtat gctgagtttg tcatggaggt ggtcaagcct tttatcgatg
    21361 agacctatcg tacaaaagca gactgccagc atacggctat gattggttcc tcactaggag
    21421 gcaatattac ccagtttatc ggtttggaat accaagacca aattggttgc ttgggcgttt
    21481 tttcatctgc aaactggctc caccaagaag cctttaaccg ctatatcgag tgccagaaac
    21541 tatcgcctga ccagcgcatc ttcatctatg taggaacaga agaagcagat gatacagaca
    21601 agaccttgat ggatggcaat atcaaacaag cctatatcga ctcgtcgctt tgctattacc
    21661 atgatttgat agcaggggga gtacatctgg ataatcttgt gctaaaagtt cagtctggtg
    21721 ccatccatag tgaaatccct tggtcagaaa atctaccaga ttgtctgaga ttttttgcag
    21781 aaaaatggta agttaagaaa ggaaaaaacg aaatgcatat tgaacatctt agccactgga
    21841 gtggtcatct taaccgtgaa atgtacctta accgttatgg acatggtggg attccagttg
    21901 tggtctttgc ttcatcaggt ggtagtcaca acgaatacta tgattttggc atgattgatg
    21961 cctgtgcttc ctttatcgag gaaggccttg tccagttctt taccctatct agtttggata
    22021 gtgagagctg gttggttact tggaaaaatg ctcatgacca agcggaaatg caccgtgcct
    22081 acgaatgtta tgtgattgag gaggccattc ttttatcaag cacaagacag gttggtttga
    22141 tggcatgatg acgacaggtt gctctatggg agcctatcat gcactcaatt tcttcctcca
    22201 gcatccagat gtctttacca aagtgattgc tctcagtggt gtttacgacg cacgtttctt
    22261 tgtcggtgat tactacaacg atgatgctat ttaccaaaac tcgccagtag attatatttg
    22321 gaaccaaaac gacggctggt ttattgaccg ttaccgtcag gcagagattg tgctgtgtac
    22381 ggggcttgga gcctgggaac aagatggttt gccatccttt tacaagctca aagaagcctt
    22441 tgacaagaaa caaattccag cctggtttgc tgaatgggga catgatgtcg cccatgactg
    22501 ggaatggtgg cgtaaacaaa tgccttattt cctcggtaat ctctatttat aaaaggagtt
    22561 acctatgaat taccttgtta tttctcccta ctatccacaa aactttcaac agtttaccat
    22621 cgaactagct aataaaggca tcacagtctt gggaattggt caagagtctt acgagcaatt
    22681 ggatgagccc ttgcgcaata gcttgaccga gtattttcgt gttgataatc ttgagaacat
    22741 agatgaagtc aaacgtgcag ttgcttttct cttttataaa catggtccaa ttgaccgcat
    22801 cgagtctcac aatgaatact ggcttgagct agacgcaaca ctcagagaac aattcaatgt
    22861 ttttggtgcc aaaccagagg atctcaaaaa gacgaaatat aagtctgaaa tgaagaaact
    22921 tttcaaaaaa gcaggtgttc ctgtggtacc tggagctgtt atcaagacgg aagcagatgt
    22981 tgatcaagca gtgaaagaaa tcggtcttcc aatgattgcc aaacctgata atggagtggg
    23041 agcagccaca acctttaaac ttgagacaga agacgatatc aatcacttca agcaagaatg
    23101 ggaccattca accctttatt tctttgaaaa atttgtcact tccagcgaaa tctgtacctt
    23161 tgacgggctc gtggacaagg atggaaagat tgtcttctca acaacctttg actacgccta
    23221 tacaccgctt gacctcatga tttataagat ggacaattct tattatgtgc tcaaggatat
    23281 ggatcctaaa ctgcgcaagt atggggaagc aattgtcaaa gaatttggta tgaaagaacg
    23341 gtttttccat attgagttct tccgtgaggg ggacgattat attaccatcg agtacaataa
    23401 ccgccctgca ggtggtttta ccattgatgt ttataacttt gctcattcct tggacctcta
    23461 tcgtggctat gcagctattg tcgcaggaga ggagttcccg gcgtcagact ttgaaactca
    23521 gtattgtttg gctacttctc gccgtgcaaa tgctcactat gtttattcag aagaggattt
    23581 gcttgccaaa tatagccagc agttcaaagt taaaaaagtc atgccagctg ccttcgcgga
    23641 acttcaagga gattacctgt atatgctgac cactccgagt cgacaagaaa tggagcagat
    23701 gattgcagat ttcggacaac gtcaagaata agaactatcg gattaaggaa attaactccc
    23761 ttaatccttt tgttttgtct gataaaaaat aagagcatcc caacaaggta gctatcataa
    23821 aacttgttcg ataactattt gaagcaggat taggtggtca gaaattgaat tttaatattt
    23881 caattgagtc atagtattgt gtttgcgtat ccttaaatca gctaaaagga tccatgacga
    23941 cacctatacg atatagtttt caagatacca aacaagtcta ttaatactca atgaaaatca
    24001 aagagcaaac taggaagcta gccgcaggtt tctcaaaaca ctgttttgag gttgtggata
    24061 gaactgacag agtcagtatc atatacctac ggcaaggtga agctgacgtg gtttgaagag
    24121 attttcgaag agtataaaat attcaggtga cgcatagata tagttaattg aagctttgtt
    24181 tgaaatctga taaaataatg atattactaa gttttaaaaa ctaaagaaaa gggaagatat
    24241 gattacaggc gaattaaaaa ataaaatcga tcagctgtgg gaaattcttt ggacagaagg
    24301 aaacgcaaat cctttaacaa atattgaaca gttgacttat ctcttattta tgaaagattt
    24361 ggatagtgtc gagcttggac gtgaaagtga tgctgaattt ctagggattc cttatgaggg
    24421 agtttttcca aaagataaac ctgaataccg ttggtcaact tttaaaaata taggagatgc
    24481 tcaggaagtt tatcgtttaa tgactcagga gatttttccg tttattaaaa atctcaaggg
    24541 ggatacagat gatacagcct tttcacgata tatgcgagaa gctatttttc aaataaataa
    24601 acctgctacg cttcaaaagg caatttctat cttagatgtt tttccaacta ggggattaga
    24661 tgtagatttt gataatgaca aacaaagtat tactgatatc ggagatatct atgaatatct
    24721 gttatcaaaa ttgtcgaccg caggtaaaaa tggacagttc cgtacacctc gtcacatcat
    24781 cgatatgatg gttgagttga tgcaaccgac tatcaaagat atcatctcag atcccgctat
    24841 gggttctgct ggcttcttag tatctgctag ccgttactta aagcgtaaga aagatgaatg
    24901 ggaaaccaat acagataata tcaatcattt tcataatcag atgtttcatg gaaatgatac
    24961 ggatacgact atgttgagac ttggggcgat gaacatgatg ctacatggag tagaaaatcc
    25021 acaaatcagt taccttgact cgctgtctca agataatgaa gaagccgata aatatacttt
    25081 ggttttagca aatcctcctt ttaagggctc acttgactac aattcaacct ctaatgacct
    25141 tcttgcaacc gtaaaaacca aaaaaacaga attactcttt ctttctcttt tcttgcgaac
    25201 tttaaaacca ggtggacgag cagcagttat cgtacctgat ggtgtccttt ttggttcgtc
    25261 taaagctcat aaaggaattc gtcaggaaat tgtagagaat cataagcttg atgctgtaat
    25321 ctcaatgcct agtggtgtgt tcaagcctta tgctggagtt tcaactgcta ttctcatctt
    25381 tacaaaaact ggtaatggtg gtactgacaa agtctggttt tacgatatga aagcggatgg
    25441 tttaagtttg gatgataagc gacaaccgat tagcgacaat gatattccag atattatcga
    25501 acgctttcat catcttgaaa aagaagcaga acgtcagaga acggatcaat ctttctttgt
    25561 tccagttgct gagataaagg aaaatgatta tgatttgtct atcaataaat ataaagagat
    25621 tgagtatgaa aaagttgagt atgaaccaac agaggtcatc ttaaagaaaa tcaatgattt
    25681 agaaaaagaa attcaagctg gcttggctga attagaaaaa ttactcaagt agggaggtgg
    25741 ctgtatgaaa aaagtgaagt tgggggaagt cttatctcta aaaaaaggaa agaaagccac
    25801 tgtacttgct gaacaaacaa ctctaagcca acgttatatt caaatagatg atttaagaaa
    25861 taataataat ttaaaattca ctgaaagttt aaatatgact gaagcactcc cagatgatat
    25921 tctaatagca tgggatggtg ctaatgcagg aacagttggc tatgggttat cgggagctgt
    25981 tggtagtaca attacggtct taaaaaagaa tgagcgatac aaagaaaaaa ttatatcaga
    26041 ttacttggga gtctttttgg aaagtaaatc gcagtattta cgagatcatt caacaggtgc
    26101 aactattcct catttaaaca agaatatatt acttgattta caattagaat tgctaggtat
    26161 cgaagaacaa gagaacatta tctgtattct taatacgatt aaaaggctta ttactaaaag
    26221 aaaatttcag ttagatgaac taaacttgct cg
//
LOCUS       DAVDWT010000028        25045 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVDWT010000028 DAVDWT010000000
VERSION     DAVDWT010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25045
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.028"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            85..3435
                     /locus_tag="VUW50_001471"
     CDS             85..3435
                     /locus_tag="VUW50_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET7983350.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDNSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIEEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDCILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(3835..5181)
                     /locus_tag="VUW50_001472"
     CDS             complement(3835..5181)
                     /locus_tag="VUW50_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HET7983351.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFRHLFLH
                     VVGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPGRKASLMMH"
     gene            5243..5437
                     /locus_tag="VUW50_001473"
     CDS             5243..5437
                     /locus_tag="VUW50_001473"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983352.1"
                     /translation="MSSKKHPLGEKRNHPGSKLRQGFWMVFRLGVNNWGFKEIENKKP
                     SKNSIAGENEIILNENQRAN"
     gene            5415..5615
                     /locus_tag="VUW50_001474"
     CDS             5415..5615
                     /locus_tag="VUW50_001474"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983353.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDKTDEVSSKHGFEVVDETDEVS
                     NHTYGKATLTRIEEIFEEYKSS"
     gene            complement(5695..6140)
                     /locus_tag="VUW50_001475"
                     /pseudo
     CDS             complement(5695..6140)
                     /locus_tag="VUW50_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6148..6291
                     /locus_tag="VUW50_001476"
     CDS             6148..6291
                     /locus_tag="VUW50_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983354.1"
                     /translation="MAERFWENLSIILADRNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            6384..6971
                     /locus_tag="VUW50_001477"
     CDS             6384..6971
                     /locus_tag="VUW50_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7983355.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCSDELTILQILYGASLIS
                     FLWMYGQRKQVIKVNMKTRIQWLGIGFVSLLIISLCFSLIHAQESTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVWNLLVYSLLLF"
     gene            7371..8180
                     /locus_tag="VUW50_001478"
     CDS             7371..8180
                     /locus_tag="VUW50_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET7983356.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNNYSCKALC
                     ECLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIREKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKVSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWNSLLLNENCLVIGASVSGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTNHLSYGNRISPQFPILLITDCLFSHYLESPERQYYYNQTIIHKEE"
     gene            8185..8886
                     /locus_tag="VUW50_001479"
     CDS             8185..8886
                     /locus_tag="VUW50_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET7983357.1"
                     /translation="MSQIWTKEKFISQVHGGVIVSCQALPGEPLYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVINEFIKKIKEKYPNQLLMADVSNFDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            8911..9828
                     /locus_tag="VUW50_001480"
     CDS             8911..9828
                     /locus_tag="VUW50_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HET7983358.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQERVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHVACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAAPNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            9856..11397
                     /locus_tag="VUW50_001481"
     CDS             9856..11397
                     /locus_tag="VUW50_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HET7983359.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGNSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGAVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLMANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNAGSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDDRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDIPEIKADSS
                     WEVRH"
     gene            11416..11874
                     /locus_tag="VUW50_001482"
     CDS             11416..11874
                     /locus_tag="VUW50_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET7983360.1"
                     /translation="MIISEQSDFRRYASINKHFSKVCDFLENTNLTDLVDGKVDIDGE
                     NVFANCMIYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDEPVKKLVIKVLNEEK"
     gene            11876..14098
                     /locus_tag="VUW50_001483"
     CDS             11876..14098
                     /locus_tag="VUW50_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HET7983361.1"
                     /translation="MKKNIKQYVTLGTAVVLSAFVANSVAAQETETSEVSTPELVQPV
                     APTTPISEVQPKSGNSSEVTVQPRTVETTVKDPSSATEETPVLEKNNVTLTGGGENVT
                     NELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNHLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPRDVGGRDLQISGGATYIDSVIVEKNNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLKKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFKNNKKTEYRNGTHVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSTDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            14138..15247
                     /locus_tag="VUW50_001484"
     CDS             14138..15247
                     /locus_tag="VUW50_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7983362.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEYEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            15404..16294
                     /locus_tag="VUW50_001485"
     CDS             15404..16294
                     /locus_tag="VUW50_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET7983363.1"
                     /translation="MKIIGIDIGGTTIKADLYNEFGTSLNQFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYILNHSIDGVGISTAGVVNANTGEIIYTGYTIPGYIGVNFTFEIEKRF
                     GLSTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRAGDKVAKETFEIFVE
                     NLTKGLLTITYLLNPEILILGGGILAKKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            16319..16642
                     /locus_tag="VUW50_001486"
     CDS             16319..16642
                     /locus_tag="VUW50_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983364.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELVSIESNLEMKQQNLIDEVNKTIKENYEKIQYISSSRRGEFVEKIIGRVV
                     EKYGY"
     gene            16632..18623
                     /locus_tag="VUW50_001487"
     CDS             16632..18623
                     /locus_tag="VUW50_001487"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HET7983365.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQISHTSNSNHGVIEGNDALIYLMNQQQYLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNRQINRVHELERHIQSNNEEIERLIKW
                     EKLEIIPAHLEQFFFCKGKVGTIPRTVDNRLYNSFLENNIEVQEIFSNDREYGVVVFY
                     QSSCSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKNNLID
                     LQWQIDYILSIYARQISKNNLLSTPHLVALEGWIEETRILYFIKVMDERFGHSIYIYE
                     SETLSDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTFFGMMVA
                     DLGYGLLLFLGTMLALKIFHLPSETKRFLKFFNILGVAVAIWGGIYGSFFGYELPFHL
                     ISTTSDVMIILVVSVVFGFITVFAGLLASGLQKVRMKKYAEAYNSGFVWCVILLGLLF
                     IAVGMLMPDMRLLFVLGKWVSIFNAVGILVVSIIQAKSLSGIGAGLFNLYNISSYIGD
                     LVSFTRLMALGLSGASIASAFNLIVGLFPGTLAKLTIGLVLFILLHAINIFLSLLSGY
                     VHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            18639..19115
                     /locus_tag="VUW50_001488"
     CDS             18639..19115
                     /locus_tag="VUW50_001488"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HET7983366.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            19149..19730
                     /locus_tag="VUW50_001489"
     CDS             19149..19730
                     /locus_tag="VUW50_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983367.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSVIVKK
                     KEVEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALIEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNHLFSEQPISNESGLLI
                     SIGKIDDNYLYKTLIGSISKEESSSIANQIFIN"
     gene            19795..20802
                     /locus_tag="VUW50_001490"
     CDS             19795..20802
                     /locus_tag="VUW50_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HET7983368.1"
                     /translation="MDTNLFSKINTTISVKENDFITEDKFQKIIQSKDTETMAFILEA
                     TPYHLSIDVLENPSQTETSLMTKLVNDYRWAYAESPSNKIVTLFALRYVYHNIKVLLK
                     SKAAIKKDFSKLLIPIGIFDIESLKHLVSSLHSDTLPDFMVHEVESIWNEYETFNNIR
                     VLDVGADLAYFKHLKLLSNELDEVLSQVIVAMIDFYNIITVKRGLSQNKSHGDILQLL
                     SDEGSISAKEFIYIVENQEIFVWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDL
                     LLYKILDQGRYNVDGPLVLARYLLGCEFEVKNLRMIISALQNTIPFGSIKERIRPHYG
                     S"
     gene            20792..21112
                     /locus_tag="VUW50_001491"
     CDS             20792..21112
                     /locus_tag="VUW50_001491"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HET7983369.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            21175..22950
                     /locus_tag="VUW50_001492"
     CDS             21175..22950
                     /locus_tag="VUW50_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HET7983370.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFSNVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGIPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGIYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            22951..24336
                     /locus_tag="VUW50_001493"
     CDS             22951..24336
                     /locus_tag="VUW50_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HET7983371.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLSSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            24359..24970
                     /locus_tag="VUW50_001494"
     CDS             24359..24970
                     /locus_tag="VUW50_001494"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HET7983372.1"
                     /translation="MVRLNVKPTRMELNNLKERLKTAERGHKLLKDKRDELMRRFISL
                     IRENNRLRKEVESYLIDNLKSFAVAKSLKNSLMVEELFSIPSKEIELFIEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIDKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
BASE COUNT         8267 a         3739 c         5052 g         7987 t
ORIGIN      
        1 ttgtgagata gctataaaag tgtggagaaa tagcttgaaa tttagtataa tatagctaaa
       61 ctatttgttt aaagtgagaa aaaaatggga aattttagct ttcttttaaa aaatgacgaa
      121 tatgaatctt tttcaaaacc ttgcattgaa gctgagaata tgattgctac atcaactgtg
      181 gctactgcct ttatggcgcg tcgtgcttta gagcaggctg tccattggat atatagtcac
      241 gattcatatt tagaagctcc ctatcgtgct actctatctt ctttagtatg ggatgatgat
      301 tttagggata tcgtagattc tgaactccac aagcagatag ttctgttgat tcggtgggga
      361 aaccatgctg ctcatggtgg tgaaattaag gaacgagaag cgattttagc tttgcatcat
      421 ttgtatcagt ttgttaattt tatcgattat tgttacagca atgagtttgt ggagcgttat
      481 tttgatgaga agtgcttacc actttcagca aacatcaaat accgagaaac tccacaatct
      541 atgataaagt tacaagacag tttaccagaa ctgcctgatt ttcatgaaca gatggctgct
      601 cagtccgtag aagttcaaga gacttatact gaaaaacgtg agactgcagc gcaacggcaa
      661 gatgtgcctt tccatattga tcaattatct gaggcagaga caagaaagct ctttattgat
      721 atcgatctcc gtttagcagg atggatattt gaagaaaact gtcgtgttga gatagccgtt
      781 gatggtctca agcacggttc aggaattggt tactgtgact atgtacttta tggtaaaaat
      841 gggaaaattt tagcgattgt ggaggctaaa aaagcctctg tcaatccaga agtaggggaa
      901 gtacaggtca aagaatatgc tgaagctttg gagaaacata tcggctatca gccaatttgc
      961 tttattacaa atgggttgaa gcactatata cttgatggtc cgaaccgccg ccagattgca
     1021 ggcttttact ctcaagaaga attgcaatta gtgatggata gacgtcatct tcaaaaaccg
     1081 cttgaggata tttctagtaa aattagggac gatatttccg ggcgtcacta ccaaaaacat
     1141 gccattgcaa gcgtttgtga agctttctct aatcatcgta gacaggcact tttggttatg
     1201 gcaactgggg cggggaaaac tcgtacagca gtttctctag ttgatatctt atcacgtcat
     1261 aactgggtaa aaaacgttct cttcttagcc gatagaactt ccttggttaa gcaagcctat
     1321 gattcgttta gaaaattact cccagatctt tccgtttgta acttcttaga agataaagaa
     1381 ggagctcaat caagtcgtat ggtcttttca acttatccga ccatgattgg agcgattagt
     1441 ggtcaagaag aagtaaatca acgccctttt actgttgggc attttgacct tatcataatt
     1501 gacgaatctc accgttctat ttatcagaaa tacaagtcca tttttgatta ttttgatgca
     1561 agaattgtag gcttaacagc tactccgcgt caagatttag ataaaaacac ctatggattc
     1621 tttaatttgg agaatggggt tccaacatat gcatatgatt tggaagaggc tgttaaagac
     1681 ggatatttag tagcctatca ttctatcgaa accaaactga aactacctac ggatggtcta
     1741 cattatgatg atttgtccga agaagaaaag gaacattttg atagcaaatt tgaagacaat
     1801 agctgtgaaa aagatattga tgggagtgta tttaattcct ttattttcaa taaaagtaca
     1861 gtagaaattg ttttaaatga actcatgaca agaggaattc agacagcctc gggtgatgaa
     1921 attggtaaaa ctattatttt tgctaaaaat catgatcatg cggaatatat cagaggtatt
     1981 tttaacaacc gctatcctga aaaagggagc gactatgctc aggtgattga ttatagtatt
     2041 aagcattatc agaccttgat tgatgatttt aaaattgagg agaagtatcc tcaaattgcg
     2101 atttctgtcg atatgttaga tacaggtatt gatgtaccag aggttgttaa tttagtcttc
     2161 ttcaagaaag tacgctctaa aactaagttt tggcagatga ttggtcgagg aacccgtcta
     2221 tgtaaagatt tatttggacc tgagcaggat aaggaaaact tcttggtatt tgattatggg
     2281 gataattttg attattttcg tgcagatcca agagatggag agggtcgtca cattgtttcg
     2341 ttgactcagc gtttatttaa tatcaaagtg gacttgattc gagaacttca gggactccaa
     2401 taccaagaag atcagtttgc gagagcatac cgtcagcagc ttgtctcgga acttcaaggt
     2461 cgtatagaga gcttaaatga gttggacttc agggttcgta tggttttaga tacagtttat
     2521 agctatagga aattggaaag ttggcagaat ctaactgctg ttacaagtga aaccattcaa
     2581 aaaaatctct ctccgctttt atttgatgaa gataaagaag atgagatggc gaggagattt
     2641 gatttgtggt tgcttcatat tcagttgggg caactgacag ctaaatcttc cactgttcat
     2701 atttcccaag tgatgaagac ggctagagct ctttctgcta ttggcaatat cccgcaggtt
     2761 tttgagcagg ctgaaattat caggaaagta caggagcctg aattttggaa agaagttaac
     2821 ttgtctgatt tggaaaaaat tcgtcttgct attcgagatt tattacagtt tttggataaa
     2881 acagaccgta aaccctacta tgttaacttt gaagattgta tactctccac tgttcacgag
     2941 accacagcat ttttgcaggt caacgatctt cggtcttaca atgaaaaagt tgagcattat
     3001 ttgaaaactc atctggatga ggagtccatt tctaagctat accataataa aaagttgaca
     3061 tctgatgata tgcttgcact tgaaaaattg ctttgggaaa aattaggtag taaagcagac
     3121 taccaaagtc attatgaaaa taaggcaatt ccgagattgg ttcgtgagat tattggctta
     3181 gatagagagt ctgccaatcg tattttttct aaatttttgt cggatgagaa tcttaatgcc
     3241 aggcagattt catttgtaaa attgattgta gactacattg tagaaaatgg ttttttagag
     3301 acgaaagtgt taacgcaaga gccgtttaaa tcttatggtt ctgttcaact actcttccaa
     3361 caccaactac cagtacttcg taatattgtt caaatcattg aacttatcaa taatcgagct
     3421 ggagaagcgg cttaaattct aaagtgattg ccatgctgag acttatttaa aattaaaaag
     3481 agtagaaatt tatgctatat atgagaagtt ttattaggaa gaatgtcatc gttttcctag
     3541 aatacagtat cagttgttaa gtggttgata aatttcaaag tagatacttg taccacgatg
     3601 tttgttgatc gagttattaa caaaagagct actttgattt taacccaaat cattcatacc
     3661 tctctcaact agatgtaact tacaaaaccc ctgacctcat gagccacttt cttcctcctc
     3721 atgaggtcag ttttactttc tgctgttcca gtatcgtttt tcctcgctag atttcctcaa
     3781 aagggcagac tcctcccttg gtgcgtcaca cgattttttc atctcgactg ttctttaatg
     3841 catcattaac gacgcttttc ttccaggtgg ttcataagga acaggaagat tcaggttgac
     3901 ttttctaatc ctagaataaa gtgctgaaaa caattcggaa taggcataga gactagacaa
     3961 tttgaggagc tgcttgcgtc ctgttcgaac acattttcct accacgtgaa gaaaaagatg
     4021 gcggaagcgt ttgattgtta aagtttggaa gtcacctcca gctagatgtt tgagaaaaag
     4081 atagagattg taggcgatac agctcatcat catacgaact tcgtttttga ttaaggttga
     4141 actatccgtt ttatcgccaa aaaatccctc cttcatctcc ttgatgaaat tctcggcttg
     4201 accacgtcca cgataaagct gaaactggtc ttggcttgtt ccactcgtca tatttgtaac
     4261 gagagaaata acatcgtaga acaagtttcc ttcttttcgt tcagagaact ggcagacacg
     4321 acgcttgtgc gaccaagatc ctgcttgata gagagtttct gagtaggcgg agtggggcaa
     4381 gatggttaag tcctcatcct gtgggcaagg gagggaaagg tctccaagac ggctcagaac
     4441 agtatttttc ttgagtttta tgaggtagta ttgccctgtt ttttcaatta aatcgtataa
     4501 ttttggggtc gcaaagccac tatccattcg aaagagaagt tgattaaacc gttctaaaac
     4561 aggtgtgata aagctgtctg cctcttcaga acaataacga ttaccaggac gaagctgggc
     4621 attgaaacaa taacctgtct tcccctcgaa agcataaaga ggatgatagc catgagcacg
     4681 atagtgggcg ttataagcaa caccttcttg cttgccataa gttgtgaaat gggtagaatc
     4741 gatatctaca atgagttggt ttagctggtg aaactgtaaa aagaattcga ccaattcaag
     4801 gttgaggcat cgcaaactat ggactgtttc ctcgtcagtt ctggaaagaa aacgggataa
     4861 ggttggctgt gaagcaagct gccctccttc caataatttt ggaaagtagg catcagctga
     4921 caattcttta caagcatagt ccgttccata acctgttaac agttgaaaga ggaactggac
     4981 aaggatatct gaatccgaat aacgacagta gcggcgttgg tcattcgtta ctaaatactt
     5041 agaaatccgc tcttttagtt tcagctggga aaaaagttcc tgaaaaaaga taagaccacc
     5101 atactgggtt aaatgacctc catcgaaaga tagttggtaa aaagacttgt tttggaagtg
     5161 atgatttggt aaactgttca tgtgagtttc ctttcttttt gtgttttttt ctacacttat
     5221 accataaagg gggaactctt ttttgtctag taaaaaacac ccattgggtg aaaaaagaaa
     5281 ccatccagga tctaagctaa ggcaaggatt ctggatggtt tttagattag gggtgaataa
     5341 ttggggtttt aaagaaatag aaaacaaaaa gccgagcaag aattcaattg caggagaaaa
     5401 tgaaataata ctcaatgaaa atcaaagagc aaactaggaa actagctgca ggctgctcaa
     5461 aacactgttt tgaggttgta gataagactg acgaagtcag ctcaaaacat ggttttgagg
     5521 ttgtagatga aactgacgaa gtcagtaacc atacctacgg caaggcgacg ttgacgcgga
     5581 ttgaagagat tttcgaagag tataaatctt cctaggataa agcaaagagc ctgggacaaa
     5641 atagttctcg accacaaaaa agctagagat ttccaattgt ggaactttag ctttttaatt
     5701 ttgagtcgtt ctcttattgt attttaggag gttattggcc ataagtacca atcccatgtc
     5761 aattctcact tgacgcttgc ccctcagatt acatctcttg taacccaaac aagcctttat
     5821 ctgcccaaag acaggtgaca ttctttagct ttcaagtttt gatagccttc gttcgtatac
     5881 agtccctttt gaggggctga ttcaggttcg tcggcgtagt aaaccttgat ttcttgttta
     5941 aagtctgtct gtgttttctg atgtttggta tggtgaaaac gataatacca gccatcagga
     6001 tgtgtatagc tatcctcctt gtcattatag taccaattcg ctaagtttct agctgactgt
     6061 ttatagcctc tcttctgttc cttatcaaac atggcatatt taatcagatg gtttacctcc
     6121 ttttcatcta actaaaggag aagcaatatg gctgaacgat tttgggagaa cttgtccatt
     6181 attttggctg acagaaatat cagctggatt gagttaacca gaaaaatgtt tgcgggagag
     6241 tttcactatc caagtgaatt gaatcgtttg tatcaaaaga ttcgccacta gaagatggaa
     6301 caacgaatgc ctcaaagtcc atgggtagaa aggattgtgc aggtattaga tttagattat
     6361 gaagatttat ttcggaggta attatgaaaa gaataattcc agtttatata ttccaacaag
     6421 taaatgtcct attggtatct ctatacttat tgaaatttct ttgtagcgat gagttaacta
     6481 tactacagat tctctatgga gcgtcactca tttctttttt atggatgtat ggtcaacgaa
     6541 aacaagtgat caaggttaat atgaaaacta ggatacaatg gcttggtatt ggattcgtta
     6601 gcctactgat tataagtcta tgttttagtc taatccatgc gcaagaaagc acgaatcaag
     6661 caaacttaat tggacttcaa catcaaattc cttggttttc atttttattg tttttaatca
     6721 atgcgagtat ggttgaagaa tttctgtatc gagaaatttt atggaatttg gtcagaaaat
     6781 tagatatcag agttgctttg acaagtgttt tatttgcctt agcacatcat ccaggaacca
     6841 ttatagcttg gtgtttgtat gtatcgctgg ggatgttttt agggcttgta cgctacaaat
     6901 tggacttatg gggcagtatg gggctacatt tggtgtggaa cctactagtt tatagtttgc
     6961 tgctttttta aaataagtga tctgatttat ctcataaatc agtagttttt ataggattat
     7021 ttttaattcg gaaacaaaaa tgtattaaca gcataaaata aagaaactag gaacattttt
     7081 tttatttttt agaaataaaa tgttgtatag aaattccgaa agaaataata aaataaaaat
     7141 aaaaattaat aaaataggta tatttgaaca tattgacgtc gacaaatgtt cttgcaagac
     7201 ataaaataca aataaaaaga ataaaaaatt tttatatttt tatatttgtt gttctgttat
     7261 aaaatgaaag aaattagaaa ctagtttcta aatcatgaaa aatattgaaa aaaaaaaaaa
     7321 taagtgttaa aataaagaat gtaaacgttt acttcaataa aggagctcat atgttactgc
     7381 aaaaagaact aattccaatg atagaagcta acttaccaaa tatggcatat gctgaaaaag
     7441 acattgctaa attcttctta aaacagcaac ctctgaataa ttattcatgt aaggcattgt
     7501 gcgaatgcct taatgtatcc aaagcaacat tgactcgatt tgcgaaaaaa tgtggtttta
     7561 aaggttttag acaattcatt ttcaaatacc aagagatgat tcgtgagaaa gaaaagttag
     7621 cattgtatac agaggctaca gaaaaagttt tatccgacta tgaagaaatg ttgagaaaaa
     7681 cttacacggt tcttgatgaa gttcaacttg agcgtattgc tgagatgata gaaactgctg
     7741 agcgtgtata tctttatggt aaagtaagtt ctgttcttgc tttacaagaa atgaagatga
     7801 gatttatgcg tctcggagtc attggtgaag tattatcaga cgaggatatg attttgtgga
     7861 atagcttatt acttaatgaa aattgccttg tcattggagc atccgtttca ggtcaaactg
     7921 atattgtact agaaggtctt caaaaagctg cggataaagg cgctaaaaca gttttaatga
     7981 ctacaagaaa atttgacgaa gaagattgtt tctttgatga actattgtta ttagcttcga
     8041 ccaatcatct ctcgtatggc aatcgcatat cacctcagtt tccaatactt ttaattacag
     8101 actgcttatt ctctcattat ctggaaagtc cagagagaca atattattac aatcaaacta
     8161 ttatccataa ggaggaataa taaaatgagt caaatttgga ctaaagaaaa atttataagt
     8221 caagtccatg gtggagtcat tgtttcttgt caagctttac ccggtgaacc cctttataat
     8281 gaagaattta gcttgatgcc ttttatggct aaagcagctt tagaggcagg agcagtgggc
     8341 attcgcgcaa attctgtgcg tgatattaaa gcaattcaga aagtagtaga tttaccaata
     8401 attggaatta tcaaaagaga ttatccacct caagaaccat atattactgc tacgatgaaa
     8461 gaagtagatg aacttgtaga atgcggaaca acagtcattg catttgatgc aactttaaga
     8521 ccaagatatg atggcttagt tatcaatgaa tttatcaaaa aaataaaaga aaaatatcct
     8581 aatcaattgt tgatggcgga tgtaagtaat tttgatgaag gtctctatgc atttaaatca
     8641 ggcgttgatt ttgttggtac aacattatca gggtacacaa gtacaagtgt acaatcagat
     8701 gagcctgatt ttgaactaat gaaaaaattg gctgatttta atattccggt aattgccgaa
     8761 ggaaaaattc attatccaga acaattaaaa aaagcttata gtttaggtgt taccagtgtt
     8821 gtcattggtg gagcgattac acgtccaaaa gaaattgctc agcgatttat taatgtcatc
     8881 aaataaaaaa caatataaag gagaaatctg atgaaagatt taactaaata caaaggcgtt
     8941 attcctgcat tttatgcttg ctatgatgaa aatggtgaaa ttagccaaga acgtgtaaaa
     9001 tctctggtgc aatatttcat tgacaaaggt gtaaaaggta tctatgtaaa tggttcttca
     9061 ggtgaatgta tttaccaaag tgtagaagat cgtaaacaaa ttattgaagc tgttatggaa
     9121 gttgctaaag gtaaattaac agttatcaac catgttgcat gtaataacac gaaagatagt
     9181 attgaattgg caaaacattc agaaagtgtt ggagtcgatg ctattgctgc tatcccacct
     9241 atttacttca aattgccaga atattcaatt gcagcatatt ggaatgcaat gagtgaagct
     9301 gcgccaaata cagattttat catttataat attccacaat tggcaggggt tgcgttgact
     9361 ggtagtttgt atgcaacaat gcgtcaaaat cctcgtgtga ttggagttaa aaattcttct
     9421 atgcctgtac aagatattca aatgtttgta gctgcaggtg gagaagatta cattgtattc
     9481 aatggtccag atgaacaata tcttggtggt cgcttgatgg gagcagaagc tggtattggt
     9541 ggtacttatg gcgttatgcc agatttgttc ttgaaattgg aaagtttgat tcaagaacga
     9601 gatttagata ctgctaaaaa acttcaatat gctatcaatg aagttatcta taagatgata
     9661 tcaggtaagg caaatatgta tgctgtagca aaagaggttt tgcgtctaaa tgaaaaactt
     9721 gatttaggtt ctgttcgtca acccttagaa gcattggcag aaggtgactt ggaagttgca
     9781 aaacaagcag cagaacttat tcaacaagca cgaaaagaat ttttataata aataaaatca
     9841 atatggagat atgttatggg cacaacagga tttacaataa ttgacttaat tatcttgatt
     9901 gtttatttac ttgcggtgtt ggttgcaggt atctatttct ctaaaaaaga gatgaaagga
     9961 aaagagttct tcaaaggaga tggttcggtt ccttggtatg ttacttcggt atccattttt
    10021 gccacaatgc tcagtccgat ttccttcttg ggactcgctg gtaactctta tgcaggtagc
    10081 tggattttat ggtttgctca attagggatg gtagtagcta ttccactgac aattcgtttt
    10141 atcttaccta tctttgcacg gatagacatc gatacggcat atgattactt ggataaacgt
    10201 tttaattcta aagcacttcg tattatttca gcactcttgt ttattattta tcaattggga
    10261 cgtatgtcta tcattatgta cctcccatca gctggtttat cagtattgac aggaattgac
    10321 atcaatattt tgattatttt gatgggtgca gttgcaattg tttattctta cactggtggt
    10381 ctaaaatccg tattatggac agactttatt caaggtgtga ttctgattag tggtgtcgtt
    10441 ttagctttat ttgtactgat ggctaatatt aaaggtggct ttggtgcagt agcagaaaca
    10501 ttagcaaacg ggaaattcct tgctgcaaat gaaaaacttt tcgatcctaa cttgctttca
    10561 aactccatct ttttaattgt gatgggttca ggctttacaa tcttgtcttc ctatgcttca
    10621 tctcaagatt tggttcaacg ttttactaca acacaaaata ttaagaaact taataagatg
    10681 ttgttcacaa acggtgtctt gtcacttgca actgcaacag tcttttactt gattggtaca
    10741 ggcttgtacg tattctatca agtacaaaat gcaggtagtg cagctagcaa tatccctcaa
    10801 gaccaaatct ttatgtactt tattgcatac cagttaccag taggtatcac aggtttgatt
    10861 ttggcagcga tttatgcagc atctcaatca actatttcaa caggtttgaa ctctgttgca
    10921 acttcatgga cattggatat tcaagatgtc atttctaaaa atatgtcaga cgatcgtcgt
    10981 acgaaaattg cacaattcgt atctctagca gtaggtttat tctcaattgg tgtttccatt
    11041 gtcatggctc actcagatat taaatctgca tacgaatggt tcaatagctt catgggactt
    11101 gtacttggtc tacttggtgg tgtatttatt cttggatttg tttctaaaaa agcaaataaa
    11161 caaggtgctt atgcagcgct gattgtatca actatcgtca tggtatttat taaatacttc
    11221 cttcctccaa cagctgtgag ctactgggca tattcattga tttcaatctc tgtatcagta
    11281 gtttcaggtt atattgtatc tgttcttact ggaaataaag tatctgcacc taaatataca
    11341 acgattcatg atattccaga aattaaagcg gattcaagtt gggaagttcg tcactaatag
    11401 aaaagagaaa ataatatgat tatttctgaa caatctgatt ttagacgata tgcttctatc
    11461 aataaacatt tttcaaaagt ctgtgatttt ttagaaaata caaatttgac agatttagtt
    11521 gatggaaaag ttgatattga cggggaaaat gtttttgcaa attgtatgat ctatctagct
    11581 gatggagtcc caggagatat ttttgaaact cataagaaat atttagatat tcatattgtt
    11641 gttgaaaata ctgaaaaaat ggcagtaact tccccagttc gtgcccagtc acgtgtacct
    11701 tttagcgaag aaaaggatat tgctttttac gatagcaaag actatcaaat tgttgaattg
    11761 cttccaggga atatgttggt gacttttgaa gaagatcttc accaaccaaa gattcattgc
    11821 aatgatgagc ctgttaaaaa acttgttatt aaagttttaa acgaggaaaa ataaaatgaa
    11881 aaaaaatatt aaacaatacg taaccttagg tactgcagtg gtattatcag catttgttgc
    11941 taactcagtt gcagctcagg agactgaaac ttctgaagta tcaacaccag agttggtgca
    12001 acctgttgca ccaacgactc cgatttcgga agtacaacct aaatcgggta actcttcgga
    12061 agttactgta caacctagaa cagtcgaaac tactgttaag gatccatctt ctgcaacgga
    12121 agaaactcct gtcttagaaa aaaataatgt tactttaaca gggggcggag aaaatgttac
    12181 taatgagtta aaggataaat ttactagcgg tgactttact gtagtgatta agtacaatca
    12241 gtctagtgag aaaggcttac aagctctgtt tggaatatct aattctaaac ccggtcaaca
    12301 aaatagttat gtagatgtgt tccttagaga caatggtgaa ttgggaatgg aagcgcgtga
    12361 tacttcttcc aataaaaatc acctagtatc cagacctgct tcagtttggg gtaagtacaa
    12421 acaagaagct gtgactaaca ctgttgcagt agtagcagat tcagtcaaaa aaacatattc
    12481 tttatatgca aatggtacaa aagtagtaga aaagaaagtg gataacttct taaacatcaa
    12541 ggatattaaa ggtattgatt actatatgct tgggggagtg aaacgtgcag gaaaaacggc
    12601 gtttggtttt aacggaacac tagaaaatat caaattcttt aatagtgcat tggatgaaga
    12661 aactgttaaa aagatgacaa caaacgctgt tactggacat ttaatttata cggctaatga
    12721 tacaacaggt tctaactatt tccgtattcc agttctgtat acttttagca atggtcgggt
    12781 attttcaagc attgacgctc gttacggcgg aactcatgat tttttgaata aaattaatat
    12841 tgctacaagt tatagtgatg ataatggtaa gacatggact aaaccaaaat taacattggc
    12901 attcgatgat tttgcgccag taccattaga atggcctcgt gatgttggtg gacgtgactt
    12961 acaaatcagc ggtggtgcaa cctatattga ctctgttatt gttgaaaaaa ataacaaaca
    13021 agtactcatg tttgcggatg tgatgcctgc tggagtaagt tttagagaag ctactagaaa
    13081 agattcaggt tataaacaaa ttgatggtaa ttattacctt aaattaaaga aacaaggtga
    13141 tactgattac aattatacta ttcgtgagaa tggtactgta tacgacgatc gtaccaacag
    13201 accaactgaa ttttcagtag ataaaaattt cggtattaaa caaaatggta attatttgac
    13261 agtagagcaa tattcggttt catttaaaaa taataaaaag actgaatatc gtaatgggac
    13321 tcacgtccat atgaatatct tctacaaaga tgcattgttc aaagtagtgc caacgaacta
    13381 tattgcatat atttctagca atgatcatgg cgaatcttgg tctgcaccaa ctttattacc
    13441 tcctataatg ggacttaatc ggaatgcgcc ttatttgggt cctggacgtg gaatcattga
    13501 aagctcaact ggacgtattc ttattccgtc ttacactggt aaagagtctg cattcattta
    13561 tagtgacgat aatggagcat cttggaaagt taaagttgtg ccacttcctt ctagttggtc
    13621 agcagaagca caatttgtag aattgagtcc aggagtaatt caagcataca tgcgtacaaa
    13681 taatggtaaa attgcatatt taacaagtac agacgcaggt actacttgga gtgcaccgga
    13741 atatttgaaa tttgtttcaa atccaagtta tggaacacaa ttatcaatca tcaattatag
    13801 tcaactgatt gatggtaaaa aggctgtcat tttaagtact ccaaactcca caaatggtcg
    13861 taaacacgga caaatttgga ttggtctaat taatgatgat aatacaattg attggcgtta
    13921 tcatcacgac gttgattata gtaactatgg atactcatat tcaacattga cagagttacc
    13981 aaatcatgaa attggattga tgtttgaaaa atttgattca tggtctcgta atgaacttca
    14041 tatgaaaaat gttgtaccat atataacatt taagattgaa gatctgaaaa agaattaaag
    14101 ctgaaatttg aaaatatata aaaagaggat aaaaattatg gtaaattacg gtattgttgg
    14161 agctggatat tttggagctg atttagctcg ctcaatgaac aaaattgaag atgcaaaagt
    14221 ggttgcggta tttgacccaa atcatggaga agaagttgct caagagttgg gatcagatgt
    14281 ttgtgcaagt ttagatgaac ttgtagcacg tgaagatatt gattgtgtga tcgtagcttc
    14341 acctagctac cttcaccgtg aaccagttgt gaaagctgct caacatggca aacacgtatt
    14401 ttgtgaaaag ccaattgcat tgtcttatga agattgtaaa gccatggttg acgcatgtaa
    14461 agaaaataat gttatcttta tggctggtca catcatgaac ttctttaacg gtgtacacca
    14521 tgctaaagaa ttgattactc aaggtaaaat cggtaaagtt ctttattgcc atgccgctcg
    14581 tacaggttgg gaagaacaac aaccaactgt atcatggaag aaacttcgtt ctcaatctgg
    14641 aggacatttg taccatcata ttcatgaatt agattgcatt cagtttatca tgggaggact
    14701 tcctgaaaaa gcgacaatgg taggaggcaa tgtatatcat aaaggtgaaa actttggtga
    14761 tgaagatgat atgctcattg taaacttaga atactctgat gatcgttatg ctgttttgga
    14821 atatggtaat gctttccgtt ggggtgaaca ctacgtcttg attcaaggaa ctgaaggagc
    14881 tatcaaactt gatttgttca atactggagg tactcttcgt gttaaaggtg aaggagaatc
    14941 acacttctta gttcatgaaa ctcaagagga agatgatgac cgtacagcta tctataccgg
    15001 tcgtggtatg gatggagcaa ttgcgtacgg taaaccagga gtacgttgcc cattatggtt
    15061 gcaaacatgt attgataaag aaatggaata tctacatgac atcattaaag gtggagaaat
    15121 tacagaagaa tatgaaaaac ttctcaatgg tgtagctgct ttagaatcaa tcgctaccgc
    15181 tgatgcatgt actttatcag ttaaagaaga tcgaaaagta agtctttcag aaatcacaaa
    15241 tgcttaactt ttgtaaaaca gaatagtaaa ttcttatcat tatataattt ctaaagttct
    15301 gtgatacaac tcattgaata aagaaataga gatgggactg ggataatgcc cagtcccatt
    15361 ttttatcaaa aagtaatgag atcaaaaatg tgggagtgat gaaatgaaga ttatagggat
    15421 cgatattggc ggaacaacaa ttaaagcaga tttatacaat gagtttggaa cgagtttaaa
    15481 tcagttcaaa gagatagaaa caattattga ctatgatttg ggaacgaacc agatattaaa
    15541 tcaggtctgt gatttaattg gtgagtatat tttaaatcat tcaattgatg gtgttgggat
    15601 ttccactgct ggagttgtta atgctaatac aggagagatc atctatacag gctatacaat
    15661 accagggtat atcggagtaa actttacttt cgaaatagaa aaacgttttg ggttgtctac
    15721 ttttgttgaa aatgatgtta attgtgctgc attaggtgaa ttgtggaagg gacaagccaa
    15781 agataagaaa aatgtagtaa tggttactat tggaacaggt ataggaggca gtattattgt
    15841 caatggacaa attgttaacg gatttaacta tactgctggt gaagtaggct atattcctgt
    15901 aggtaattcg gattggcaaa gcaaagcctc aacaaccgca ttgattcatt tatatcaaaa
    15961 gaaaagcttg aaaactaatc aaactgggcg tactttcttt actgatttaa gagctggaga
    16021 taaagtcgct aaagaaactt ttgaaatttt tgtagaaaat ctaacaaaag gtttattaac
    16081 gattacttat ctacttaatc cagaaattct catattagga ggtgggatcc tggctaaaaa
    16141 ggatattttg ttacctgaaa ttcaaagttc tttagctaaa aatgcaatgg ataataggtt
    16201 tttgcctaaa aatcttgtgg cagctacatt aggaaatgaa gctggtcgta taggagctgt
    16261 aaaaaatttc ttagatagaa tttctaataa atagtatgaa agataaggag gtgtcacaat
    16321 gactaactct gtattttcga caatgcaaga tattgagaat gttgcaaccg atattataaa
    16381 atcatatgat aatgagattt atacttataa agctgtttcc caagaagaat tggaaaaact
    16441 agaaaaaagt tatgatgaaa aaagtcacga agaattagtt tcaatagaaa gcaatttaga
    16501 aatgaaacaa cagaacctta ttgatgaggt taataaaaca atcaaggaaa attatgaaaa
    16561 gattcagtat atttcatcaa gtaggagagg agaatttgta gaaaaaatta ttggtagggt
    16621 ggtagaaaaa tatggctatt agtcagatga aaagaatttc tctactattt tctaaaagta
    16681 gtcttgatga tgttttaaaa actattcaag aactagagtc agtgcagttc cgtgatttaa
    16741 aggttcagga taactggtca gaagctctag aaaaagatga ggttgtattt ccaactattc
    16801 aaatttctca tacttctaat tccaatcatg gggttattga aggaaatgat gccttgattt
    16861 atttgatgaa tcaacaacaa tatttagaag caactgtaga gaaattacaa gaatacctac
    16921 cgaaagaaaa cacgtttaaa ttattacagc aacctccgat aacgacctct tatgaagaat
    16981 tagaaaaatt tggtaaagct aatgttgctg agggtgttct taaaaaagtg aatcgtcaaa
    17041 taaacagagt tcatgaatta gaaagacaca ttcaaagtaa taatgaggaa atagagagat
    17101 taataaaatg ggaaaaatta gaaattattc ctgcgcattt agaacaattt tttttctgta
    17161 aaggaaaagt cggaacaatt ccaaggactg tagataatcg cttatacaat agttttttag
    17221 aaaacaatat tgaagttcaa gaaatatttt ctaatgatag agagtacggt gttgttgttt
    17281 tctatcagtc cagttgctct attgattttg atgaatactt atttgaacca tttgattatt
    17341 ctagaaagga attaccgaag cagcgagtag tagatttgga tcaagaaaac atgcagttaa
    17401 taactgaaaa agagaatatt atcgcatcgt tgcaagattc aaagaataat ttgatagatt
    17461 tacaatggca aattgactat attttatcta tctatgctcg tcaaatctct aagaataacc
    17521 ttttgagcac tccgcatcta gttgcactag aaggatggat agaagaaact cgtattttat
    17581 attttataaa agttatggat gagcgttttg gacattctat ttatatttat gaatcggaaa
    17641 cattgtcgga taatcaagat gaaataccta tcaaattaac gaatcattct ttaattgaac
    17701 catttgaatt attgacagaa atgtatgctc tgcccaaata ttatgagaaa gatcctacac
    17761 ctgtattagc accattttac tttacatttt ttggaatgat ggttgctgat ttaggctatg
    17821 gtttactatt gtttttagga acaatgttag cattaaaaat ttttcatcta ccttcagaaa
    17881 ctaagagatt tttaaaattc tttaatatat taggggtagc cgttgcaatt tggggtggaa
    17941 tctatggctc attttttgga tatgagttgc catttcatct gatatctaca acctctgatg
    18001 tcatgattat attagtagtg tcagttgtgt ttgggtttat tacagtattt gcaggtttgt
    18061 tagcttcagg actacaaaaa gtaagaatga agaaatatgc agaagcatat aattcaggat
    18121 ttgtgtggtg tgttattctg cttggcttgt tatttattgc tgtcggaatg ttgatgcctg
    18181 atatgagact gttatttgta ttagggaaat gggtatctat ttttaatgct gtgggaattt
    18241 tggttgtttc tattattcaa gccaaaagct tgtcaggtat tggagcagga ttgtttaatc
    18301 tatataacat ttcatcttat ataggtgatt tagttagttt cactcgattg atggcattag
    18361 gattatctgg agcaagtata gcatcagctt tcaatttaat tgttggtttg tttccgggaa
    18421 cattggctaa actaacaatt ggattagtat tattcattct tttacatgcg atcaatattt
    18481 ttctatcgtt actatcagga tatgttcatg gagcacgtct gatatttgtt gaattttttg
    18541 gtaagtttta tgagggtgga ggaaaaccat ttcaaccttt gaaggcttct gagaaatata
    18601 ttaaggttat tacaaagaat taatggagga tatatataat ggaacattta gcaacttatt
    18661 tttcaaccta tggaggagct ttcttcgctg cattgggaat tgtattggcg gttggattaa
    18721 gtggtatggg gtctgcttat ggagttggta aggctgggca atctgccgca gctttactga
    18781 aagaacagcc agaaaagttt gcctctgctt tgatattgca attactgccc ggaacacaag
    18841 gattatatgg ttttgttatt ggaattttga tttggttgca attaactcca gaacttcctt
    18901 tagaaaaagg cgttgcttat ttctttgtag ctcttccaat tgctattgta ggatactttt
    18961 cggctaagca tcaaggaaat gtagcagtag cgggaatgca aatcttggct aaaagaccaa
    19021 aagaattcat gaagggagca attttggctg ccatggtaga aacctatgca attcttgcct
    19081 ttgtcgtatc attcattttg acccttcgtg tataagaaat taatttgcta ttcaaaggag
    19141 gtgtctaaat gagcaattta gaaaacttac gagagtctgt tattgaacaa gctcatgaaa
    19201 aagggcgtat gaaattattg gattccaaaa agaagattga tgatgaattt gaaatgcaaa
    19261 agtcggtcat tgtaaagaaa aaagaagtgg aacatgaacg aaagttaaaa gaattacaac
    19321 agaaatatca aataattttt caacaattaa aaaataagga acgccaatca acgttagtat
    19381 caaaacagaa aatattaaaa gaactttttc aatctgcttt aatagaaatg gaatcttgga
    19441 gtgcagataa agaaatggag ttcatctatc gaattctgga acgatattca caacaagagg
    19501 tcatagtaac ttttggggag cggactttag ctaaattcaa tctggaacaa ttagagaaat
    19561 tgaaattctc ttttccaaat catttattta gtgaacagcc tatctcaaat gaatcaggct
    19621 tacttatttc aataggtaaa attgatgata actatttgta taaaacatta attggatcga
    19681 tttctaagga agaaagttca agtatcgcaa atcaaatttt tatcaattaa ggatgaaatt
    19741 ggttaatcct tcttagaaat ttggagtatt ccaataaaat tagaaaggta ttttatggat
    19801 actaatcttt tttcaaaaat aaatacgacg atttcggtaa aagaaaacga ttttattaca
    19861 gaagataaat ttcaaaaaat tatacaatcc aaagatacgg agacaatggc atttatctta
    19921 gaagcaactc cctatcattt atcgattgac gttttagaaa atcctagtca gacagagact
    19981 tcgctaatga caaaattagt caatgattat agatgggcct atgctgaaag tccgtctaat
    20041 aaaattgtga ctttatttgc tttacgatat gtttatcata atatcaaagt tttattaaaa
    20101 tctaaggcgg caattaagaa agatttttct aaattattaa ttccaatagg gatttttgat
    20161 atagaaagtt taaaacattt agtttcttcc ttacattcag atacacttcc tgattttatg
    20221 gttcatgaag tagaatcaat ttggaatgag tatgaaactt ttaataatat tcgtgtactt
    20281 gatgtcggag ctgatctagc atattttaaa catctgaaac ttttatctaa tgagttagat
    20341 gaggtgctgt ctcaggttat tgtcgcaatg attgactttt ataatattat tactgtaaaa
    20401 cgtggtttat ctcaaaataa gagtcatggg gatattttac aattactttc agatgaagga
    20461 agtatttctg ctaaagaatt tatatacatt gtagaaaatc aagaaatatt tgtgtggttc
    20521 aataaaataa atccaagctt agattcaatc ttttcaactt atgaattgaa gatgcaggac
    20581 gcaacaattt catcttctga gttagaattt ttatgtgatt tattattgta taaaatttta
    20641 gatcaaggaa ggtacaatgt agatgggccg ttagtacttg ctagatattt attgggatgt
    20701 gagtttgaag taaagaatct cagaatgatt atatcagctc ttcaaaatac aattcccttt
    20761 ggatcaataa aagaaaggat acgcccgcat tatggaagct aataagtata aaattggcat
    20821 aattggtagc cgtgatatta ttttaccctt tagcatgatt gggtttgata tatttcctgc
    20881 ctatcaagaa caagaagcta taaatactct aagaaaatta gcccaatctg attatggtgt
    20941 catttatatc actgaagaca ttgcttcaat gatattagat acaattcgcc attatgattc
    21001 tcaagttgtg cctgctatta ttttattacc gactcataaa caaggtttaa atttaggatt
    21061 aaaacgtata gaggataatg tggagaaagc agtaggacac aatattttat aataatgtac
    21121 aaaattgtct gtaatattat tctataattt ttggacttag taaggagaat aactttgact
    21181 caagggaaga ttataaaagt atcgggacct ctagttattg catcaggtat gcaggaggct
    21241 aatattcaag atatttgccg tgtaggtaag ctagggttaa tcggtgaaat tattgaaatg
    21301 agaagagatc aggcatctat ccaagtctat gaggaaacat ctggtcttgg tccgggagaa
    21361 cctgttgtta caactggaga acctctctcg gttgaattag gaccaggatt gatttctcaa
    21421 atgtttgatg gcatacaacg cccattagat cgatttaaat tggctactca taatgatttt
    21481 ctagttcgtg gggtagaagt tccaagtttg gatagagata ttaagtggca ttttgattcc
    21541 actatagcaa ttggtcaaaa agtgagtacg ggtgatattc taggaactgt caaggaaacc
    21601 gaggtagtta atcataaaat tatggttcct tatggagtat ctggcgaagt cgtttctatc
    21661 gcatctggcg attttacaat tgatgaagtt gtatatgaaa taaaaaaatt ggacggtagt
    21721 ttctataaag gaacgcttat gcaaaaatgg cctgttcgca aggcgcgtcc tgtttctaaa
    21781 cgtttaatcc cagaagaacc attaatcaca ggtcaacgag ttattgatac attctttcca
    21841 gtaaccaaag ggggagcagc agctgttcct ggaccgtttg gagcaggaaa gacagttgta
    21901 caacaccaag tagctaaatt ttccaatgtt gatattgtta tttatgtcgg ttgtggagaa
    21961 cgtggaaatg aaatgacgga tgtactgaat gagtttcctg agttgattga ccctaatacc
    22021 ggacaatcaa ttatgcaacg gacagttctg attgctaata cttcaaatat gcctgttgct
    22081 gctcgtgagg cttcaattta tacaggaatt accatggctg agtattttcg tgatatgggc
    22141 tactctgtcg ccattatggc tgattcgact tcacgttggg cagaagcact acgtgaaatg
    22201 tcaggacgtc tagaagaaat gcctggtgat gagggttatc ctgcttatct gggaagtcgt
    22261 atcgctgaat attatgaaag agcgggacgt tctcaggttc tagggattcc agaacgtgaa
    22321 ggaactatta ctgctattgg agctgtatcg ccacccggtg gagatatttc agaaccagtt
    22381 actcaaaaca ctttacggat tgtgaaagtt ttttgggggc ttgatgctcc gttggcacag
    22441 agacgtcatt ttcctgcaat taactggctt acatcttatt cactctataa agacagtgtg
    22501 ggaatttata tagatggtaa agagaagaca gattggaata gtaaaataac tcgtgcgatg
    22561 aactacttac aacgggaatc tagtttagag gaaattgttc gtcttgttgg aattgattct
    22621 ctgtctgata atgaacgact aacgatggaa attgctaaac aaattcgaga agattatttg
    22681 caacagaatg cttttgattc agtagataca ttcacttcgt ttgcaaaaca agaagcaatg
    22741 ctaagtaata tcctaacttt tgctgatcag gcaaatcatg ctttagagtt aggttcttac
    22801 tttacagaga ttatggaagg taccgtggca gttcgagacc gtatggcgcg aagtaaatat
    22861 gtttcagaag atagattaga tgaaatcaaa attatttcaa atgagattac acatcaaatt
    22921 catttgatat tagaaacagg aggtctataa atgagtgtta taaaagaata cagaactgct
    22981 agtgaagttg ttgggcctct tatgattgtt gaacaagtaa ataatgtgtc ttacaatgag
    23041 ttagttgaaa ttcaacttca taatggagaa attcgtcgtg gacaagtttt agagattcac
    23101 gaagataaag caatggttca actttttgaa ggatctagtg gaataaattt agaaaagtct
    23161 aaaattcgtt ttgctggtca tgcactagaa ttggctgtat ctgaggatat ggttggtcgt
    23221 atttttaatg ggatgggaaa accaattgat ggtggaccag atttaattcc agagaaatat
    23281 ttagatattg atggtcaagc tattaatcct gtatctagag attatccaga tgaatttatt
    23341 cagacaggga tctcctctat tgatcatttg aatactcttg tacgtggtca aaaattacca
    23401 gtattttcag gttcgggctt acctcataat gaattagcag ctcagatagc aagacaagcg
    23461 actgttttaa attctgatga aaattttgcg gttgtatttg cagcaatggg tattactttt
    23521 gaagaagctg agttttttat ggaagaactc agaaaaacag gagcgatcga tcgttcagtc
    23581 ttatttatga acttggcaaa tgatcctgca attgagcgta ttgcaactcc ccgcattgct
    23641 ttaaccgcgg cagagtatct agcttttgaa aaagatatgc acgttctagt tatcatgacg
    23701 gatatgacta actattgtga agcgttacgt gaagtctcgg cagcgcgccg tgaagttcca
    23761 ggtagacgag gctatccggg atatttatat acaaatttat caactctata tgaaagggct
    23821 ggtcgcttag ttggtaaaaa aggttcagtg acacagattc ctattttaac aatgccagaa
    23881 gatgacataa cacatccaat tcctgattta actggataca ttactgaagg gcaaattatt
    23941 ttgtctcatg agttgtataa tcaaggttat cgtccaccaa tcaatgtttt atcttctctc
    24001 tctcgattaa aagataaggg atctggagaa ggtaaaactc gtggagatca tgctccaact
    24061 atgaatcaac tgtttgcagc ctatgcccaa gggaaaaagg ttgaagagtt agcagtagta
    24121 ttaggagaat cggctttatc tgatgtagat aaattgtatg tgagatttac aaagcgtttt
    24181 gaagaagagt acatcaacca aggattttat aaaaatcgaa atatagaaga tacgttgaat
    24241 cttgggtggg aattactatc aattcttcct agaacagagt taaaacgtat caaagatgat
    24301 ttgcttgata aatatttacc tttggtagaa gtttaatcca gaaatggagt gattatctat
    24361 ggtacgtttg aatgtaaaac caactcgtat ggaattgaat aacttaaagg aacgtttgaa
    24421 aacagctgaa cgtggacata agttattaaa ggataaaaga gatgaattga tgaggcgatt
    24481 tatttctttg attcgtgaga ataatcgact tcggaaagaa gtagaaagtt atctaattga
    24541 taatctaaaa tcttttgcag ttgctaaatc attaaagaac tctctaatgg tggaggaatt
    24601 attttcaatt ccatcgaaag aaattgaatt atttattgag aaagaaaata tcatgagtgt
    24661 aacagttcct agaatgcata tgaatattac ttctcaaaat gagaacagtg aatacagcta
    24721 tttatcttct aatagtgaaa tggatgatgt atttgctaca atgaatagtt taattgataa
    24781 attactaaga ctggcagaag ttgaaaaaac gtgtcagtta atggctgatg aaatagaaaa
    24841 aacacgtaga cgtgtaaatg gtttagaata ttcgattatt ccaaacttgt cggaaactat
    24901 tcattatata gaattgaaac tagaggaggc agaaagagcc aatttagttc gtattatgaa
    24961 agtgaagtag ttcctttatt tagattttta attagatgaa caaatatcag cttggataag
    25021 gcggtaagcc tttctaagct ttttt
//
LOCUS       DAVDWT010000029        24963 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVDWT010000029 DAVDWT010000000
VERSION     DAVDWT010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24963
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.029"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..171
                     /locus_tag="VUW50_001495"
     CDS             <1..171
                     /locus_tag="VUW50_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET7983373.1"
                     /translation="IEQVWKEIRKRGFKNKAFRTLEDVMNQLQDVIQGLEKEVIKSIV
                     NRRWTRMLFESR"
     gene            complement(288..635)
                     /locus_tag="VUW50_001496"
     CDS             complement(288..635)
                     /locus_tag="VUW50_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7983374.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEIEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(746..2521)
                     /gene="spxB"
                     /locus_tag="VUW50_001497"
     CDS             complement(746..2521)
                     /gene="spxB"
                     /locus_tag="VUW50_001497"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HET7983375.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(2728..4971)
                     /locus_tag="VUW50_001498"
     CDS             complement(2728..4971)
                     /locus_tag="VUW50_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET7983376.1"
                     /translation="MTEIVKASLENGIQKIRIRSEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQTLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(4981..5352)
                     /locus_tag="VUW50_001499"
     CDS             complement(4981..5352)
                     /locus_tag="VUW50_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET7983377.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHTNLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            complement(5363..5758)
                     /locus_tag="VUW50_001500"
     CDS             complement(5363..5758)
                     /locus_tag="VUW50_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HET7983378.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      5942..6040
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6124..6915
                     /gene="thiD"
                     /locus_tag="VUW50_001501"
     CDS             6124..6915
                     /gene="thiD"
                     /locus_tag="VUW50_001501"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET7983379.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQAKDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(7124..7756)
                     /locus_tag="VUW50_001502"
     CDS             complement(7124..7756)
                     /locus_tag="VUW50_001502"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET7983380.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(7749..8552)
                     /locus_tag="VUW50_001503"
     CDS             complement(7749..8552)
                     /locus_tag="VUW50_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET7983381.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLNYRPTVIKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSKNQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEAYE"
     gene            complement(8552..9076)
                     /gene="thiW"
                     /locus_tag="VUW50_001504"
     CDS             complement(8552..9076)
                     /gene="thiW"
                     /locus_tag="VUW50_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HET7983382.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(9081..9773)
                     /gene="tenA"
                     /locus_tag="VUW50_001505"
     CDS             complement(9081..9773)
                     /gene="tenA"
                     /locus_tag="VUW50_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HET7983383.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKEMEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(9784..10434)
                     /locus_tag="VUW50_001506"
     CDS             complement(9784..10434)
                     /locus_tag="VUW50_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HET7983384.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10436..11821)
                     /locus_tag="VUW50_001507"
     CDS             complement(10436..11821)
                     /locus_tag="VUW50_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7983385.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(11822..12382)
                     /locus_tag="VUW50_001508"
     CDS             complement(11822..12382)
                     /locus_tag="VUW50_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET7983386.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(12644..12732)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(12941..13570)
                     /locus_tag="VUW50_001509"
     CDS             complement(12941..13570)
                     /locus_tag="VUW50_001509"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET7983387.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(13572..14354)
                     /gene="thiM"
                     /locus_tag="VUW50_001510"
     CDS             complement(13572..14354)
                     /gene="thiM"
                     /locus_tag="VUW50_001510"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET7983388.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEIAEKRPNGHLPGTFKAEFINALYEITDEDVKEFKRVK"
     gene            14445..14531
                     /locus_tag="VUW50_001511"
                     /pseudo
     CDS             14445..14531
                     /locus_tag="VUW50_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(14681..15349)
                     /locus_tag="VUW50_001512"
     CDS             complement(14681..15349)
                     /locus_tag="VUW50_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HET7983389.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYNV"
     regulatory      complement(15564..15652)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15906..17042)
                     /gene="lctO"
                     /locus_tag="VUW50_001513"
     CDS             complement(15906..17042)
                     /gene="lctO"
                     /locus_tag="VUW50_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HET7983390.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(17667..19157)
                     /gene="lysS"
                     /locus_tag="VUW50_001514"
     CDS             complement(17667..19157)
                     /gene="lysS"
                     /locus_tag="VUW50_001514"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET7983391.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINKPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(19285..19431)
                     /locus_tag="VUW50_001515"
     CDS             complement(19285..19431)
                     /locus_tag="VUW50_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HET7983392.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TSFQ"
     gene            19464..20381
                     /locus_tag="VUW50_001516"
     CDS             19464..20381
                     /locus_tag="VUW50_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275026.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7983393.1"
                     /translation="MYIAVWGSLGSFLLGLIVSIIRHYRIPVLAQVATAYIELSRNTP
                     LLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSAMQDSGIQVLFQGNNLLRILQGL
                     GVTIGISILSVLLSMMFGTVMGIIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFG
                     LARNFNINISGETSVIIVFTLWGTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYY
                     HIIIPQVLRRLLPQAINLVTRMIKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTTSFW
                     IYGTILILYFAVCYPISKLSTHLEKHWRN"
     gene            20382..21140
                     /locus_tag="VUW50_001517"
                     /pseudo
     CDS             20382..21140
                     /locus_tag="VUW50_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            21152..22012
                     /locus_tag="VUW50_001518"
     CDS             21152..22012
                     /locus_tag="VUW50_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7983394.1"
                     /translation="MKLFKPLLTVLALAFALIFITACSSGGNAGSSSGKTTAKARTID
                     EIKKSGELRIAVFGDKKPFGYVDNDGSYQGYDIELGNQLAQDLGVKVKYISVDAANRA
                     EYLISNKVDITLANFTVTDERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLI
                     VTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGF
                     EVGITSLGDPDTIAAAVQKGNQELLDFINKDIEKLGKENFFHKAYEKTLHPTYGDAAK
                     ADDLVVEGGH"
     gene            complement(22092..22739)
                     /locus_tag="VUW50_001519"
     CDS             complement(22092..22739)
                     /locus_tag="VUW50_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7983395.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKPGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(22736..23434)
                     /locus_tag="VUW50_001520"
     CDS             complement(22736..23434)
                     /locus_tag="VUW50_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983396.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLSLLCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            complement(23444..24196)
                     /locus_tag="VUW50_001521"
     CDS             complement(23444..24196)
                     /locus_tag="VUW50_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983397.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(24196..24960)
                     /locus_tag="VUW50_001522"
     CDS             complement(24196..24960)
                     /locus_tag="VUW50_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983398.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDTESHFLTAQLCRISKKKYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGILWAILGLSYLEPVTTLALGY
                     QKDISLSLVSAHLAFILFVSCLVVYGTFFLHSEDYV"
BASE COUNT         7738 a         5386 c         4500 g         7339 t
ORIGIN      
        1 attgaacaag tgtggaaaga gattcgtaaa cgtggattta agaataaagc ctttcgaact
       61 ttggaagatg tcatgaatca actccaagat gtcatacaag gattggagaa ggaggtgata
      121 aagtccatcg ttaatcggag atggactaga atgctttttg aaagcagatg agtattatat
      181 gcaatttctt tatataaaaa gaccggattg ctccgatctt tcaatagttc atattctcaa
      241 tttctatttt aaaaatagct aaggttaacg tcaaatgact acgcgaccta tttcatacga
      301 taaaaatcaa gcactagacc agcaggtcct tgaactaata aggactctgt tccccaatcg
      361 gttacagttg gtccgtgtaa aacctttata ccaagctcgt tcaaccgttt gtagttctgg
      421 tctacatcct caatctcgat atgaataatg attcctgact gaaagttttc caaaggaacc
      481 aaatgatttt gtgacaacat aaggcagtga ctaccaatcg taaactgagc aaaaccatca
      541 tcagcataat ctgccttttt atccaagata tgctccaagt cagcacagac ttggggaaca
      601 tttgaaacga taatatctaa ttgatttaaa ttcatttact ctcctccata aaaagaccgg
      661 attgctccga tcttttaaag ttctgctcta tgaaaatcaa agaataaagt ctacaagttt
      721 catatttgat tttcggcgag aggaattatt taattgcgcg tgattgcaat ccttcttctt
      781 ccaagaagag acggaatggt acgagttctt ctgcttcgta tttttccttg aaggctttga
      841 tagcttcttc tgagtgaagt tttggatcca attcaagtac ttctactgga agtggacggt
      901 gttgagtgat gcgagcatcg atgacaacag ttttaccttc tttgttcaat ttaacagctt
      961 ctgcaacaac tgcatcgatg tcttcgatac ggtcaactgt gaatccaaca gctccttgag
     1021 cttccgcaat tttagcgtag tcagcgtttg tgaagtctac accaaacaag tgtttgtttg
     1081 tatcttcgta tttgttcttg atgaagccgt actcagcatt tgagaagaca aggttgataa
     1141 ctggaaggtc gtattgaacg tttgtgataa cgtctgggta gcacatgttg aatgctccgt
     1201 cacccatgat gttccatact tggcgatctg gattgtcttt cttagcagcg ataccaccag
     1261 gaagggcaat acccattgtc gcaaagagtg gagatgtacg ccacatgttc ttaggtgtca
     1321 tgtgaaggtg acgagtagat gtttgagtag tgttacctac gtcgattgag tagatagcgt
     1381 cttgatcagc atgtttgttg attgcattgt aaacttgata caattgcaat tcaccctcag
     1441 ttttaccttc gagtttgttc atgtaatcac gccagttttg gttgttctta acgtttgcac
     1501 gccaccatgg agttgattca actgggttta ctttgtcaag gatagcttta gctgcttgac
     1561 cagcatcacc aaggattgaa gcgtcaagag catgacgttt accaagtttg taagggtcga
     1621 tatcgacttg gataaatttt tcagtgttct tgaatgcttc gtaaacttca gcgaatggga
     1681 agtttgaacc aaggaaaaga actgtgtctg cttcaaagac cacttcgttg gctggtttcc
     1741 aaccaacacg gtaagcagaa cctgtcaaac cttcatagtt ccattcgaaa gcttcaaagt
     1801 ttttaccagt tgtgatgatt ggtgctttga ttttacgtga caattcagta atcacttcac
     1861 cagctttaac accaccaaat ccagcataga taactgggcg ttcagcattg ttcaagattt
     1921 caacagcttt gtcgatttca acttcgttca aagcaggagc gatgaatgag cgttcgtatg
     1981 aacctgaacc gtagtatgag ttttcatcga tttcttggaa accgaagttt actggaattt
     2041 caacaacagc tggacctttt ttagaaactg cagcacggca ggcttcgtca attacttttg
     2101 gcaattgctc agcgtaagct acacgtttgt tgtaaacagc gataccgttg tacattgggt
     2161 tttgattaag ctcttggaaa gcatccatgt tcaattcgtt aactggacgt gatccaagga
     2221 tcgctaggaa tggagtgtta tccatagctg catcgtaaac accgttaatc aagtgagtcg
     2281 cacctggacc acctgaacca actgcaaccc cgattgagcc gccgaattta gcttgcataa
     2341 ccgctgcaag agcacctgtc tcttcgtggc gaacttgtaa gaaacggata tctttgtctt
     2401 cagccaaagc atccatcaat gagctgagtg ttcctgatgg gataccgtag attgtatcta
     2461 cgccccatgt tttcaatacg ttaagcattg ctgcagatgc agtaattttc ccttgagtca
     2521 taatgataac tctccttcaa tttttttaaa cttggagaat acgattacat agaattggaa
     2581 acgttctcca aatttttact attccactgt atcatattta tgctgacttt tctaaaaatc
     2641 tgctcaaaac tctctattct ctattctaat acagttttga aagtgttgtc gtttctgttt
     2701 tataacaaag aaatctagtc attactttta gtctatttta ctaaaattta acagaaggga
     2761 actggtcaga acagatacag aactaaaggc catggctaga cctgccaatt ctgggttgag
     2821 agccagtcca acacctgaaa agactcctgc tgcaatcgga attccgacaa cattgtagat
     2881 aaaagcccag aaaagattga gtagaattcg atgaaaggtt ttcttactca tatcaaaggc
     2941 acgaaccact cctaaaagat tattggttgt caacaccaaa tctgctgact cgatggcgat
     3001 atctgttcca gcacccatag caattcccac atctgctaca ctaagggcag gagcgtcatt
     3061 gataccgtcc ccaacaaagg ctactttccc tgactgttgc agtttatgga tttcatgggc
     3121 tttttcttct ggcaagacgc ctgcaatgac ctcttcaatt ccgatttgat ctgcaatagc
     3181 acgcgccaca ccagcattgt ctcctgtcag catgactgtt cggagaccac gtttttttag
     3241 ctgactgatg gctagcttag cattttcctt aggaatatct tgcaaagcaa gcaagccttt
     3301 gatttcattg tcaacagcta agaacacaac tgtcttagct tctttttcta gttcttctag
     3361 tttatcttga taagtattag aaatatccat gccatccagc attttagcat ttccaagtaa
     3421 aacttgtttt ccattgattc gccctgaaac acctttcccg tgcaaggtct gaaaattttc
     3481 aacagtttga aactcaagtc cagcttcact cgctcgctta acgatagcct cagccagtgg
     3541 gtgttgagaa gcatcttcca aggaggctgc caacccaaac acttctactt cgtcgccgat
     3601 gacatctgtt accacaggtt tcccttccgt caaagtcccg gtcttatcaa agacaagggt
     3661 ttgaactttc tggatttcct gtaagacagt tccatttttg aggagaaccc ccatcttggc
     3721 actacgtcct gtccccacca taagggctgt cggtgttgca agtcccaagg cacaaggaca
     3781 ggcgataatc aaaaccgcca ctccgtagag aagagaggac acaaagctag ctccaagcac
     3841 aaccacacta tccctgagca agacgaacca aacccaaaag gtcatgattc ctaaaatgac
     3901 aactactggg acaaaaatcc ctgaaatctt atccgtcaag tcctgaatcg gcgcacgact
     3961 tgtctgagct ttcttcacaa aatccacaat ctgagccaaa acagtctctg agccaacttt
     4021 ttctgctcta aagacaagcg ttccactatg attgatggtt gagccaatga cagtatctcc
     4081 aactgtcttg tccacaggca gactctcacc tgtcaccatg gattcgtcaa tactagagac
     4141 accttctact acgacaccat caacagcaat cttttcaccg ggacgcactc gaatcaggtc
     4201 gcctaccttg acttgttcca aaggaacttg gacataacta tcatcactca agacttctgc
     4261 ggttttagct tgcaagtcca gtaatttctc cacagcttgg gacgtatttt ttctcatttt
     4321 ttcctcaaaa actgctccca aaagaacgaa aaagaggata aatccagcac tttcgaagta
     4381 aacagggaga ccagcaaaga gagcaactag gctatagaaa taagccacta gagttcccag
     4441 cgcaaccaag gtatccatgt tggcattgtg ctttttaaaa ctggcccaag cactctggat
     4501 atatggctta cctgcaacta acataatagg cgttgttgct agaaaggttc cccaatgcat
     4561 gacttgatga ctaatgctac ctgtcaacat cccaatcatg agaatcacaa gaggcacagt
     4621 aaagatacta gtaatccaaa aacgttgcag gagagataga gattttcgag tcttctcaac
     4681 gactgtatag cttccctttt gcatcttcat gccacaagaa aattcatgtc gccctaattc
     4741 ttgaggtgta aaacgaatga ctttctcctc atctacgccg attggttcca agataccttc
     4801 ttcttcaaac agaatttcct tataacagtt tgaaggagta gcacgatgaa aggtaatctc
     4861 agctggaatt cccttttgaa gctggatatg ggctggatga tagccttttt cagatcggat
     4921 acggattttt tgaatgccat tttctaagct tgctttcaca atttctgtca tagtctccac
     4981 ctactctaca atcatcttgc cgtgcatcat gttcatacca caagcaaagc caaattctcc
     5041 agcctgttca ggcgtgattt ccactacata ctcttccccc attggcaggt tcgtatgtac
     5101 accaaaatct ggaaaaacaa tttgatccag acatggtgaa ggatccttgc ggtcaaagac
     5161 aatgcgggct ggcactgatt tcttgaggac aatcaactca ggagtatagc ctcccatgac
     5221 ttccactcga atctcttggt atccgttttt ttgctgggct ttttgtccag atttttcagg
     5281 ctttttgaaa aaccaaaaca agataaacgc gataagggca atacaaataa tggttacaat
     5341 actatttaac atgacgtctc ctttacatac aattacatct tacttctgtt acagcacttg
     5401 atttcttctc tgaaatcaca gcttccaagt cttccaagtc agtctgagta aattcacatt
     5461 ctacaatcaa gtcagccaac aaattcctaa tcctacggga acaaaccttg tctttgatat
     5521 cttggacaag taaatcccga ctttggtcta gagttaaaag ggctgaataa acaaaggact
     5581 tgccttcttt tttccgagtc aaacactctt tctcaaccag acgagccaaa agtgtctgaa
     5641 ccgtggactt ggaccagtca aaccgctctg ccaaaaccct aatcaaatct gtactggtct
     5701 gctccccctg catccaaata atcttcatga cctgccattc tgcatctgaa atctgcatta
     5761 ccatacctcc aaaatctaca tttgtcaatt acactcatca gtatactctt aaaatctaca
     5821 tttgtcaatt atagaaataa tattttcttc gaaaaataga attttaatca tttgaaaaac
     5881 gatttgcagt caaatattac tatataaaca ataaaaatat gctatactaa agaaaaaaga
     5941 aaacaaccac taggggtgcg taaagctgag attaacgact gttagatccc tctgactcaa
     6001 tctaggtaat gctagctgat ggaagtggaa atgataatgg ggactagcag tcttctattg
     6061 cctttctaaa acagactagc ttgttcttaa gaatacaaac ttcagttggt tgggaggttt
     6121 tagatgactt atttacccgt tgctttgacc attgcaggga ctgaccctag tggtggtgct
     6181 ggcattatgg cagatttaaa gtcattccaa gcgaaagatg tctatggaat ggctgttgta
     6241 accagtcttg tcgctcaaaa taccagaggt gttcagctaa tcgagcacgt ttctcctcaa
     6301 atgttgaaag cccaattgga gagtgtcttt tctgatattc cacctcaggc tgtaaaaact
     6361 ggaatgttgg ctactactga aatcatggaa atcatccaac cctatcttaa aaaactggat
     6421 tgtccctatg tccttgatcc tgttatggtt gctacaagtg gagatgcctt gattgactca
     6481 aatgctagag actatctcaa aacaaactta ctacctctag caactattat tacgccaaat
     6541 cttcctgaag cagaagagat tgttggtttt tcaatccatg accccgaaga catgcagcgt
     6601 gctggtcgcc tgattttaaa agaatttggt cctcagtctg tggttatcaa aggcggacat
     6661 ctcaaaggtg gtgctaaaga tttcctcttt accaagaatg aacaatttgt ctgggaaagc
     6721 ccacgaattc aaacctgtca cacccatggt actggatgta cctttgctgc agtgattact
     6781 gctgaactag ccaagggcaa gagtctttac caggcagttg ataaggccaa ggcctttatc
     6841 acaaaagcta ttcaagatgc ccctcaactc ggtcatggtt ctggtccagt caaccataca
     6901 acttttaaag attaagaaaa aaactctcta gttcccactt taagggaatt agagagtttt
     6961 tatacccttc gaaaatctct tcaaactacg tcagcttcca tctgcaacct caaaacactg
     7021 ttttgagctg acttcgtcag tcttatctaa aacctcaagg cagtactttg agcaacctgc
     7081 gactagcttt ctagtttact ctttgatttt cattgagtat taattaggaa agaatgtcat
     7141 gcaactttgt taaaaaggct tgcgtttttg cctcaatatc ttctgcttgc atcaaatcac
     7201 gtacaacagc tacaccagct atgccagtgc ccataagctg atcaatattc tccgaagtca
     7261 agccgccaat agcaactatt ggaatggcaa ccgtttggca aattgttttc aaggttgaaa
     7321 tcagggtgat gggtgcattt tccttggtgg ttgtcggaaa aatggctcct gtccccaagt
     7381 aatctgcacc tgatttctcc gcttccagag ctcttttaac cgttttagcg gtgacaccga
     7441 ggattttttc aggacccaag acttgtcggg caaccgaaac tgggagttcg tcatctccaa
     7501 tatgcagacc agcagcatca accgcaagac aaacatccaa ccgatcatcg attatcaagg
     7561 gtacctgata ggcatctgtt atttccttga cttgttttgc cagttgataa tattgattgg
     7621 ttgtgagatt tttttctcgc aattggacta tggtaacccc tgaacggcag gccgtctcaa
     7681 cttttgcaag aaagctttcc acggaatctt gatagcgatt ggttaccaga tatagtctaa
     7741 gtgcttctct attcataagc ctctcctttg atggtatcta gccaattttc atctcttctt
     7801 aggagcgaaa gctgattgag tacttggtaa cgaaattctt ccaatcccat tccttgaaca
     7861 actattttct cagcagcgat attgagataa gagactgcta agcaagaagc ttcaaaacca
     7921 gtctttcctt ggctgagaaa aacggctgtt aaggctccaa ccaaatctcc tgtccctgtt
     7981 atccagtcta attcagtaca gccattctcc agtacagcga cctgattttt cgaaacgatg
     8041 aggtccttgg ggccagtaac taagaatgac ataccaggat aggtctgaca ccagtctttc
     8101 aagacttgaa gcaaatcctc cgtttcttga tctttagcac tcgcatcgac cccaacgccg
     8161 tggtgcttta agccaacaag acttcgaatt tctgacatgt ttcctttaat gaccgtaggt
     8221 ctatagttta aaaggtcttt aactaagctc ttacgaatgg atgaagtcgt tacgccaacc
     8281 gcatctacta ccatcgggag agaagattgg tttgcatacg aagctgccat gcggattgct
     8341 ttttccttct cagctgacaa atgccccaaa ttgatgaaaa gagcctgact ttgcttagta
     8401 aaatcaagaa cctcacgggg atcatctgcc atgacaggtt tgcatcccag agccaaaatc
     8461 ccatttgcca gcatctcaca agaaatctca ttggtcatac agtgaattag ggaactagag
     8521 cctataggaa agggatttgt aaattcctgc atcagtctat cctttcacta aagaaatatc
     8581 cctgcacttt tttaaagaat tcctgcttga ttaaaaatcg aaaggcaata aaggaaatcg
     8641 ctgtaccaat caaggttgct ccgaaaaatc gaggcgtgta gataaaccag ctaagcttag
     8701 cagctgatcc tgtaaagagt accataacag gataggaaac aatggaacca ataatacctg
     8761 ttcccacaat ttctcccaag gcagaaaaat aaaattttcg accgtactta taaaagagac
     8821 ctgccagaag ggctccaaaa gtcgctcctg tgagagctaa aggcggaatc ccttgagtcg
     8881 tcatacggat aaaggctgtg actgtagcca tggccaaggc ataaacaggt cccatcatga
     8941 ttcccgctag aatattgact acactggaca tcggtgccat tccctcaatt cgaaagatag
     9001 gtgtaaggac tacatcaagg gcaatcatca tagataaaat ggtcaatttg tgaacttgta
     9061 gttggtgctt tctcatgttt ctattcttct cctttttcta aagactgtaa atcgctcttc
     9121 catgtctggt gttggtaagc catctcccaa aacttggctt ccatatgaac actgatgtgg
     9181 aaggcatcta gcattttttg cttatctgtc tcatcacttt ctcgatagag ctgattgacc
     9241 agtgctccct cctctctgat ctgttgctct aactcatccg taatataagt ttcaatccat
     9301 tgttgataga gaggatttgg tgatggttta agattaagtg atttgcctat atcatggtat
     9361 aaccaaggac aaggaagcaa gcttgcaaaa gctatggcta agttcggttc tgcaaattgc
     9421 ctataaatat gagaaatgta atgataacag gttggagcaa ttggatgttg ctccatttcc
     9481 tggtcgctga tttccatttc cttgaaaaat tgttggcgaa taaataactc accctccact
     9541 aaaccctgag cattttgttt caagagtctt ttcatctctt ggtttgaagt cttatcagcc
     9601 aaaagatgat agatttctga gaaggccttc agatagtagg catcctgaat caggtaatag
     9661 cggaaaatgg caggttctaa attcccctct tgtaattgta aaataaaggg atgatgaaag
     9721 gaagcctgcc aagctttctt ggataattcc atcgcaatat ctgtaaattc cataataact
     9781 cctttataaa aatagactgg tttgaagcaa taaaaagaaa agcaggtaga ttaattttgt
     9841 ttttttagga atataaaaag tccgatagct attcttcaac tgtgcatgtt cgtcatatcc
     9901 atgagcagac agagctctca ggtaaagatg gcgccaccta aagactgtca tcagaacctt
     9961 actgtaaatc aagggcgacc aaaaatgcag ttcttgacca cgtaataggc aagcttcttt
    10021 gagggacttg atttcttgct gaatgagagg aaaggaattg aataccacaa tcaaggcata
    10081 ggaccaagag cgtgatagcc ccttttgagc caagtacaag agaagctctt ttagtgaaat
    10141 agaggaaaca aagacaaggc cgatacaaac tgtcacaaag gccctcgttc caagcatgac
    10201 tgcctgtgaa gcatctccgt gtaactgaac tgcccagtag ttggcaaaag atggtaaaat
    10261 ggcaagtatg atcatccaag ctaacatttt aaatcgacgg taatagagca taaagagaat
    10321 acaaaatgcg actaccgaaa gattcagagc aatcgaagga atgaaagatg tttccaagga
    10381 taaaatcagc aagaagagac tgataatcgg tgtctgggtt gctactttga ccatactatc
    10441 tcacctcccc ttgggtattg ctactctgag atgtaagtgg tttggtaatg gtcacttctt
    10501 tcacatgccg aagaccctga ctagtcatct caatccaata atcaaccaca gaaatcaaag
    10561 ggtctaaacg atgactaatg agcagaaaac ttcttccttg attcctctcc tccacaatcc
    10621 acttgcaaaa ataatggcag gctctatcat ccaaacctgc aaaaggttca tctagcaaga
    10681 tcacggaagc cttactggtc aagatggtca agagctgaag aattttttgc tgaccaccac
    10741 ttaattgata gggactctta tcgactgcct gctccaaatc aaaatatcgt aaagcttgaa
    10801 aaatccgctg atttctttca gaatcaggtc catctaattg aagctcctct cgcagactga
    10861 ctcggataaa ctgcttctca gcttcctgaa caacaccagt cagatcacga tacaaactct
    10921 ttttcttttt caggactgtc cccttccatg caatgcgccc cttatacttt tgaaattgaa
    10981 gaatagagcg aaagagggtt gatttcccga caccattgtc acccaggata caggaaatcc
    11041 cttgatagaa tgtgaaatca gcaattgaaa agagggggcg attaccaagc tcaccagtca
    11101 cacggttcat atggaatagt tccgggctag aagcaacttc ctttgaagca acctgtgtca
    11161 tctcatagga agggatttga aacacttccc ttagttttcc gtctcttagc tcaaccatat
    11221 ggtcgatata ggctttatag tcagataaat catggtcgca caaaataact gtcttcccat
    11281 caagagccaa ctcttttaga atctccaata tctcgattct gctcttgcgg tcaatagaag
    11341 cgaagggctc atccaagaga tagaccctag gattcatggc aaagaggaca gccagcgctg
    11401 ctttttgctt ttccccacct gataggtgat gaatgggacg gtgtaagatt gcctcgcaac
    11461 gacattgctg gacaacctct gctattttag aatcaatttc ctgaaggtga tagccgatat
    11521 tctccaaggt gaaaatcagc tcctcaaaca agttctccat ggtaaattga tgattgggat
    11581 tttgaaagag aatgccaacc ttctggacac gtgcgatgat agaaagctga ctgacctcgc
    11641 tcccatctat caggacttga ccactatagg gaagagaact gacttgggca atgacttgaa
    11701 aaaggctgga ttttcctgaa ccactgctcc caactaacaa ggtaaaggct tgcgcatgaa
    11761 aagtaaaatc aaacggctca gagaagattg gggactgaat cgctcgtagt tccagaccca
    11821 tctatgcttt tcctccagtt gcaaactgat gatagagttt gacaatggca cgaaccaaga
    11881 tggtacagaa gaaataaaca gaaataaaac gtaccacaag taaggaaagg acaaacggaa
    11941 gggaaaaggc gtagtaacct aacttaatgt attcatagac aaagctaaca agcgtaatcc
    12001 caatactatt agcagttaga gagagccaac tttcatagcg attcttagtt acgataaaac
    12061 caaattcact tcccaaacct tgaaccaagc cagacaaaag agctcctaga ccaaattggc
    12121 taccataaag gacttcagca agcgcagcta gcacttctcc aatcgttgca cttccgactc
    12181 tcggaacaaa gatggcagca atgggcgcag ccatacacca gagaccgaag aggatttcat
    12241 tggcaaaggc ctgcaaacca agaggtgtta agagtagact gagaatatta tacacatatc
    12301 ctgaaccaac gaaaacccca ccaaaaaaga tagacaagaa agcaagcaag ataacatctt
    12361 ttaactgcca ttttttcaac ataaaaaact ccttttttaa aagaaaagtg gggcactcaa
    12421 gaagaccgac ctaaatactt tgtatagcag attgaattta gaacagtaca caggaatgct
    12481 aaaatatttc tagaaattaa tttgaatttt ctaattgatt tgttcgcatc ttatttcaat
    12541 ctactatatc atcttcatcc agtttcgtaa aagaaaaaac tctaattaca gatacgaatt
    12601 agagttcagc ttacaagatt agacagttct tttcgacata cgaaaaaaac atttcacatt
    12661 tcccttcgcc agtcttaact gtatcaggtt caatgggtat catctcagcc taaagcaccc
    12721 caaatgcctt tattatttaa ttatgtgatt attataacac acattttata ctagttcaag
    12781 aaattgaact ggaaatacag ccttgcactc acaaagacaa cagatctttc ttttgcaaaa
    12841 aacaaatgac ctgtttgatg aattagccat tcaagctgaa tctagacata gctttttaaa
    12901 aaaggaaaat cctacttact tagaatccaa ggatagatat ctattgttca ctcatttccc
    12961 gaacagtttt ttctatattt tttgcatacg atattgccga aatgattgaa acgccgtcaa
    13021 cattggtctt tataatgtct ttaatatgtt tcgtctgtat cccaccaatt gcaactaaag
    13081 gcatttgtgg caatagtttt ctcatcaatt caagaccttc ataacctata gcaccaccag
    13141 catcatcttt tgactgggta tcaaatacag gcccaacacc tacataatct acatattcaa
    13201 cttttgattg ttgaaattct tcttcgtttc ttatagaaag accaattatt ttatctggca
    13261 tcaattttct aatttcatca acaccaatat catcttgacc tacatgtacg ccatcggcgt
    13321 caatttccat tgctaaatct atatcgtcat taacgataaa tggaacattg tattttttac
    13381 aaagttcttt aatttggata gctagctcaa gtttttctaa gccttctaaa gcaccctcac
    13441 ctttttctcg aaattgaaat aaggttatac caccttttaa ggcttcctca acgactgtat
    13501 atagattttt tccttggcaa gtagtcgttc cacaaataaa atatagtttt agtaattctt
    13561 tatgaaacat cttacttcac tcttttgaat tcctttacat cttcatctgt aatctcgtat
    13621 aaggcattta taaattcagc tttaaatgtt ccaggaagat gtccattggg acgtttttct
    13681 gctatttctc cagcgatatt gtaaaccaac attgctgttt ctaatgattt caattcttga
    13741 cctttttcta gtccgataaa gcttgctact acagctccta ataagcatcc tgtcccaatg
    13801 actttcggca tcatagcact accattatga atcgttacca cttctccatt aacagcaatg
    13861 gcatccactt cacctgttac tactattgga atattgaact tctcatttgc tgctagagca
    13921 atttcgtcaa tattatctac gcccgcacta tctactcctt tagatgccac atctattcct
    13981 actaaagagg caatctcgcc agcatttcct ctaatcgctg ctagtttata attgttgatt
    14041 agatcatccg ctactttttt tctatattct cctgctccac aggctacagg atctaaaact
    14101 gctgggacat tatatttctc cgcaattttc agagcagctt ggtataattt ccaattttca
    14161 tctgtcaatg ttcctatgtt tattaataaa ccaccagcat actttaacaa atcctctaaa
    14221 tctgctggaa actcactcat ggctggtgag gcgcccagtg ctactaatcc atttgctgtg
    14281 aaatttttta ctacatcatt ggttatacaa atgaccaatg gtgctttttc ttttaataat
    14341 tttaaacttg tcatattgaa atccttcctt ttcactttat acgatctact gatttcgatt
    14401 tatatctatc tttcgttaag aaattttttt catttacatt gaatgattta tactcaatga
    14461 aaatcaaaga gcaaactagg aagctaaccg caggttgctc aaaacactgc tttgaacctg
    14521 tagataagac tgacgaagtc agctcaaaac actgttttga ggttgtggat agaactgacg
    14581 aagtcagtaa ccatacctac ggcaaggtga aactgacgtt gtttgaagag attttcgaag
    14641 agtcttacct catcaaattt gtaaatatca tgagccttct ctagacattg taaccaatat
    14701 caaaaaaagc taattctaaa gcgactgctt gattccagcg ttgctgaagt tctgtcaaat
    14761 cttctcgatt tttaccgaca cgattgagtt cgtcaaccag aaattgaacc cactctgcaa
    14821 agaaaggacc tctgtggaga ttgatccatt ccgaatgaat atagacttca ggtagagcca
    14881 aatctttaga accccagtct aaatagagac cttctgcaat gaccagcata accaaaagat
    14941 gggcatagtc tgatgaagcc actgccgaat acattagatc ctgaaaggct tttgttacag
    15001 ggtgcaaagt cacttctaga tagtcattct ctgctacttt taactcttta aaagcctttt
    15061 ggaaataacc atcttcatct gcttcaagaa agcctagttg cttggcaaaa cgaagcttgg
    15121 attcaagttt atctgcgtga gctacgcagg cacccagcat ggataagaag gcatcaaaga
    15181 agtgataatc ttgaatcaga tagtccttta agaccttatt ctcaattgtc cccgcaaaaa
    15241 gttccttaac aaaacgatga ttgattgcag cctgccaatc cttctgactg ctttttaata
    15301 attctccaac agtcaaacct ggctgaaatg catagtcttg tgtttccata tttacttctc
    15361 ctctctttac ttgttagtaa ttaataaaac accaagaaat atcaagcaaa atcgtaattc
    15421 cacttgatcc ttttaaagca catcgagagc atttgcagag agctaactaa acaagcctat
    15481 ccagtttata taaacaaaaa actccaatta caatcaagaa ttagagttga cttacaagat
    15541 tagaccgttc atttcaccat acgaaaaaac tgttcacatt tcccttcgcc agtcttaact
    15601 gtatcaggtt caatgggtat tatctcagcc taaagcaccc caaatgtctc tattatttaa
    15661 ttactgaacc agtatagcaa aaaatgaaag ccctagcaag atatttgacc gaaaaatatc
    15721 tttatatata atatattgaa actagaatag tacacctcta cttctaaaac attgttagaa
    15781 atcgatttaa ctgtcctgat taatttgtcc tattcttatt tcattttatt atagttttcg
    15841 atagcgattt attcttccaa tacacgaaga aaaacctcca cattcagtgg aggcaatctg
    15901 ttttatcaat acaattttaa gtcacgaggg tcaactggga aggttgggtt gtatggattg
    15961 tgacggagct taaagtgttt gacatcttca atggtctgag ctccagacaa ttgcataact
    16021 gtcttcaatt ccgcattcaa gtgttcaaag acttgacgca caccgacact accaccgaga
    16081 gccaagccat aaataacagg gcgaccaata gctaccaagt ctgctcctga agccaaggct
    16141 ttaaagacgt gttgaccacg acgaacacca gagtcaaaga caatcggcac acgtctatca
    16201 actgcttctg ccacttcttg aagcgagtca aaggcagctg gtccaccgtc gatttgacga
    16261 ccaccgtggt tggttaccca gataccagaa gctcctgcag caagtgaacg ttcaacgtcc
    16321 tcacggcatt gtggtccctt gacatacaca ggaagaccag agtattcagc gataaattct
    16381 acatcgcgtg gagacaagcg ttgtttagct gatttgtaaa caaagtccat tgatttacca
    16441 gcaccttctg gcaggtattc ttcaacaatc ggcatgccaa ctgggaagac aaaaccatta
    16501 cgcttatcca cttcacgatt cccccctaca gtagcatctg ccgtcaagac aatcgcttta
    16561 taaccttcag ccttcacacg gtccatgatg tggcggttga taccgtcatc cttactaaag
    16621 taaaattgaa accaatgagg tgtcccttga agggcttcag aaatctctgg aaggtcaaca
    16681 gtagagtaag aactggttgt ataaagagaa ccaaactcat gcacaccacg cgcagtcgcc
    16741 acttccccct gttcattcgc taatttatgg gccgcaacag gcgccataat gattggtgaa
    16801 gatagttttt cacctgcaaa ttcaatctct gtacttggat tttcaacatc gcaaagtgta
    16861 tgaggaacga tgagtttgtg gttaaaggcg cggatattct ctcttaaagt gaaagtatct
    16921 tccgccccac tagcgatata gccaaatgct gctttaggaa taacttgttg cgccattggc
    16981 tccaaatcat aggtattgat gaagtctaca tgaccttctg cattgcttgt tttgtatgac
    17041 ataaaatgcc ctccttgatt aagtaagcgt ttgcttttta ctacacaact ataataactc
    17101 ttttttcaat cattttcaag agttttgctt aaaaaccatc ttattttttc atacaatata
    17161 ctagccctac tttccttaaa ctctaaactt tagtctatat taagtgaatc gttgagattt
    17221 tttgctgcca ggcatttgaa ctactttttt atttgcggat tctatctact aatactataa
    17281 aaaaacaagt gaataagaca ccagaacatc ctctaatagg acctatggac ttatatcagt
    17341 cccatatata ctgtttaagc aagatactat cctagttgac gctaacttcc tatcccactt
    17401 attaattttt tatttatagt gaatctgaac aaatcaattg gggaatccaa gtcaatttct
    17461 agtacttttt gaacagtttc agcgtactat cgtaaaatga aataaaacat gcacaaattg
    17521 attagggaat ttaaaccaat ttctaacagt gtcttagaaa tcaaagtgta ctatttcaac
    17581 ttcaatatac tacacttcat tttctcctta aattcagtct ctttttgaat caaaaaatcc
    17641 cctggtaaga gccagagggt aagaatttat ttcattgttg ggaagagcaa tacatcacgg
    17701 atagttgttg tatcagtgag gagcatgcag agacggtcga taccgattcc caaaccacct
    17761 gttggtggca taccgtattc aagagcctcg atgtagtcat agtcgatgcc tgtcgcttca
    17821 tcatcaccaa gttctttagc tttagcttgg gcttcaaaac ggctaagttg atcgattggg
    17881 tcgttcaact cagtaaaggc attaccgtac tccttagtca tgataaagag ctcgaaacgg
    17941 tcagtaaagc gttggtcttc aggatttttc ttagcgagtg gagatacagc tactggatgt
    18001 ccatagacaa aggttggttg gattaaagtt tcttcgacaa actcttcaaa gaaggcattg
    18061 atgatgtgac caacctcagt gtagtgtttc tcaactggaa ctttcttctc agcagcgata
    18121 gctttagctt cttccaaagt catgtcttgc cagaaatcga caccagtaat ttctctgata
    18181 gcatccacca tatgaacacg cttaaatggt ttgttaatct tgatttcagt accttggtag
    18241 ttgactgggc catcaccttt gactgattta gcagcgtgtt ggataatgcc ttccgtcaag
    18301 tccatgatgt cttggaaatc tgcataagct tggtaaactt cgatagaagt aaactcaggg
    18361 ttatgagtag cgtccattcc ttcgttacgg aagatacggc caatttcata gacacgttcc
    18421 ataccaccca cgataaggcg ttttaagtga agctcagtcg cgatacgaag caccatgtca
    18481 atgttttggg cattgtggtg ggtgataaat ggacgggcag cagcaccacc ggcttcatta
    18541 tgaagaacag gtgtttccac ttcaaggaat cctttttggt caaggtaacg acggatttca
    18601 gagatgattt ttgaacgagt gacaaagcgt tcaaagcttt cacgattaga aatcaagtca
    18661 aggtaacgtt tacggtaaat tgtttcaacg tctgtcaaac catggaattt ctcaggaaga
    18721 ggacgaagag ccttagacaa gtgtgtgatg tgggttgcct tgatagagag ttctcccata
    18781 tccgtacgca tcacttcacc ttcgacacca aggaagtcac caaggtctgc ttttttgaag
    18841 atttcgtagt tttcttcacc gacagcatcc ttacgaacat agatctgaat ctggccttcg
    18901 cggtcttgaa ggtgggcaaa accaactttt cctttaccac gtttggttac caagcgtcct
    18961 gcgatagtag ctgtttcgtt tttatcgtgt aattgttctt tatcgaggtt ggcatattta
    19021 tcttttaatt cttgtgaatt tgcagtacgt tcaaaacgtt ttccgaaagg atcgattcct
    19081 tgttcgcgga gcgcagccat tttttcacgg cgaacgatct gctggtcatt tagttcttcc
    19141 atatgttctg tagacatggg atcctcctag ttttactcaa aattgaatac gatgagtcat
    19201 tttttgcgat acccccattt tatcataaat aagcaagaaa ttcacggata atctttaaaa
    19261 actaaaaaga acctgacgct aaaatcactg gaaagaagtg aaagtatctt ccgccccact
    19321 agcgatatag ccaaatgctg ctttaggaat aacttgttgc gccattggct ccaaatcata
    19381 ggtattgatg aaatctacat gaccttctgc attgcttgtt ttgtatgaca taaaatgccc
    19441 tccttgataa gtaagcgttt actttgtata ttgcagtttg gggaagtttg ggatcctttc
    19501 tgctcggttt aatcgttagt atcatccgac attatcgaat ccctgttttg gcgcaagtag
    19561 cgacagccta cattgaattg tcacgtaata cgcccctttt gattcaactc ttctttctct
    19621 acttcggtct tccccgaatc gggattgtcc tatcttcaga agtctgtgca acgcttgggc
    19681 ttgtcttttt aggaggctcc gccatgcagg attcgggaat ccaagtactc tttcaaggaa
    19741 ataacctcct gagaatctta cagggattgg gcgttacgat tgggatatcc atcctgtctg
    19801 tcctcttatc catgatgttc ggaacagtca tgggaatcat catgacctcc cattctagaa
    19861 tcatacgatt tttaacacga ttgtatctgg aatttatccg tatcatgccc cagctggtgc
    19921 tactcttcat cgtttacttt ggcttggctc gaaactttaa tatcaatatc tcaggtgaga
    19981 cttcagttat tatcgttttt accctctggg gaacagctga aatgggagac ttggtacgtg
    20041 gagctatcac ttctctccct aaacatcagt ttgaaagtgg acaggcactc ggcttgacta
    20101 atgttcaact ttactaccac atcatcatcc cacaagtctt gagaagactg ctaccgcagg
    20161 ctatcaatct tgtcactcgg atgattaaaa ccacttcatt agttgttttg attggggttg
    20221 tggaagtgac caaagttgga caacaaatca tcgatagcaa tcgcctgacc atcccaacta
    20281 cttcattttg gatttatgga accattctaa tcttatattt cgcagtttgc taccctattt
    20341 ccaaactatc cactcactta gaaaaacatt ggagaaacta aatgtctgaa actatcttag
    20401 aaatcaagga actaaaaaaa tccttcggag acaatcccat cctccaagga ctttctctag
    20461 aaatcaaaaa aggggaagtt gttgtcatcc tagggccatc tggttgtggg aaaagtaccc
    20521 tccttcgttg cctcaacggc ttagaaagta ttcaaggtgg agatattctt ctggatggtc
    20581 agtctatcgt tgaaaataaa aaagattttc acctagttcg ccaaaagatt ggcatggtct
    20641 tttaaagtta tgaactcttt ccccatctgg atgtcttaca aaacctcatc ctaggcccta
    20701 tcaaaactca aggatgggac aagaaagaag taacggaaga agctttgcaa ttactagatc
    20761 gtgtcggttt gctggataaa caacatagct ttgcccgtca attatctggt ggacagaagc
    20821 aacgtgttgc aattgtccgt gccctcctaa tgcatccaga aatcatcctt tttgacgagg
    20881 tgactgcttc gctggatcca gaaatggtgc gtgaggtgct ggaacttatc aatgatttgg
    20941 cccaagaagg ccgtaccatg attttagtaa cccacgaaat gcagtttgcc caagccattg
    21001 ctgaccggat tatcttcctc gaccaaggga aaatcgctga agaaggaaca gctcaagcct
    21061 tctttaccaa tccgcaaacc aaacgagccc aggaattttt aaacgtcttt gactttagcc
    21121 aattcggctc atatctataa aggagattct tatgaaacta ttcaaaccac tcttaactgt
    21181 tttagcactt gcctttgccc ttatctttat cactgcttgt agctcaggtg gaaacgctgg
    21241 ttcatcctct ggaaaaacaa ctgccaaagc tcgcactatc gatgaaatca aaaaaagcgg
    21301 tgaactgcga atcgccgtgt ttggagataa aaaaccgttt ggctacgttg acaatgatgg
    21361 ttcttaccaa ggctacgata ttgaactagg gaaccaacta gctcaagacc ttggtgtcaa
    21421 ggttaaatac atttcagtcg atgctgccaa ccgtgcggaa tacttgattt caaacaaggt
    21481 agatattact cttgctaact ttacagtaac tgacgaacgt aagaaacaag ttgattttgc
    21541 ccttccatat atgaaagttt ctctgggtgt cgtatcacct aagactggtc tcattacaga
    21601 cgtcaaacaa cttgaaggta aaaccttaat tgtcacaaaa ggaacgactg ctgagactta
    21661 ttttgaaaag aatcatccag aaatcaaact ccaaaaatac gaccaataca gtgactctta
    21721 ccaagctctt cttgacggac gtggagatgc cttttcaact gacaatacgg aagttctagc
    21781 ttgggcgctt gaaaataaag gatttgaagt aggaattact tccctcggtg atcccgatac
    21841 cattgcggca gcagttcaaa aaggcaacca agaattgcta gacttcatca ataaagatat
    21901 tgaaaaatta ggcaaggaaa acttcttcca caaggcctat gaaaaaacac ttcacccaac
    21961 ctacggtgac gctgctaaag cagatgacct ggttgttgaa ggtggccatt aactcttaaa
    22021 agggactgga ttttaagctc caatcccttt ttaagatttt acctataaca tcctgagtct
    22081 atctaagatg ttcaatctga acacagtgta catactttat cttctattgc atatacttta
    22141 tcacataaga tacgaatatc ctcttcacta tgactagcaa tcaaaattgt tgtccctttt
    22201 tcactagaga gctttctaaa caatgttctc atattttcta cacttgattt atccaaggca
    22261 ttcataggtt catctagtaa aagaatagag ggattctcca taattgcttg agcaatccct
    22321 agctttttcc tcatacctag cgaataagtt ttaactttct ggtctttttg ctcatataga
    22381 ccaactattt tcagtgtatc attgatttcc tgattaccaa ctactcctcg tatgcttgcc
    22441 aaatattgta aattcttaaa gccactataa taatttataa aaccaggttc ttcaatcaaa
    22501 gctcccaaat tagctggaat ttttctctca ggaacaatat tttccccatt gattaacact
    22561 tctccataag acggactata taaaccagct attaatttaa acaatacact tttccctgag
    22621 ccattcgcac cagtgattcc tatgatttcc cctggtttac aactaaagtt aaggttttga
    22681 aaaacacatg tcttttttaa tttcaactca atatttttta atatgattat ttcattcatt
    22741 ctataaacct cctcttttga cgagtgaaat agaaaatact tttaaaaaga aagactaaaa
    22801 atagcaactg aagaaataaa tctcgtccta tatctccatt ccctcgattc aaaatataaa
    22861 atagataatt agttcgattc cctacaaata gaccaccaaa cacaatcatg agtaaaaaga
    22921 aactaacgca agcaaagttc atttgtacat tcgctcctag tgcgtataac ataaagcaaa
    22981 gcaaggatag cacacaagca ttcaaagaat caactataaa caaaactcct atatcccagt
    23041 ttggtaactg taacataaat tgaatcacta gaacttttac catgattaaa atgagtccat
    23101 aaagaaaaca ggtccaaatc tgatacctaa tccagagctt aatagaagaa aatcgaatca
    23161 cttccataaa gaaatcggat gctcttctac gatatacaac tgctagaaaa aatgtatgaa
    23221 taataaacca atgaagcaac catatggata agactaaaaa agagttttga ctcagattag
    23281 gcccttttaa aatttctata acaagtctat catttccttc taaccaatac cataatccta
    23341 aacaaatcaa tatccaaaaa agaacataac cccaaaagta cttggtatag attatctgat
    23401 tcaacctcca catgtgacta cttaactttc ccatttccaa cttttacact ctcctccagt
    23461 ttctatgaac agaaaataca gtcaatggaa ttatgataga tagtaatgta agccaataaa
    23521 tcaatgcata cggattggaa tttaccatat actgaaaaat tggcaataat ccaattatcc
    23581 cattgtccaa ctgataaatc cagaataagg attctacaaa taaaaaagta aacagaaata
    23641 ctaagctcat gtttgatttt ttaaaccaag aaaaactgct aatagtaagt aaggataaaa
    23701 agaaatagta tgctatataa gagaaaaaaa atcctataaa gaaactagca ttgtttgcaa
    23761 tacttatacc ataaaattct cttaaaaaat caacctcctt tatctccaaa gagaagctaa
    23821 aaccattact aaatgcaatt agaaaaatca ataaaaataa agtcgccgtc caaatccccg
    23881 tactaagagc tgctaatttg aaactaaaac tggtaaagtg cttaattgat ttcagacgaa
    23941 tgcgacactc caacctatta aaatagttat tcatcaaata aaaaaagaac aatatatatg
    24001 tgaacggaaa gcaatatact ccagtcgtca tatcttgaag taaaactaag atccattcta
    24061 atacatttgg atggattgaa tattggcgac agcgcaataa atatactgta ctagataaaa
    24121 cacaggatag cagtaatata aaataaacca atactgctaa gaaatctttt ttataaaatt
    24181 gaacaaattg tttcattata catagtcctc tgaatgtaga aaaaatgtac cataaacaac
    24241 caaacaacta acaaataaaa taaaagcaag atgagcacta actaaggaaa gactgatatc
    24301 tttctgatat cccaaagcta gcgttgtcac aggttctaag taagatagcc ctaaaatagc
    24361 ccataaaata ccaccaacca tcatataggc aactgggatg aaaatagctc ctattttttt
    24421 cttcactagc aaagcactag ctagtccaaa aatagagaac acagcgcccc aaactccata
    24481 ccagagagtc gtcacaagac tatagagcaa ctgattggaa tcaaataatt cttttaaggc
    24541 accactataa tctccaatat aaatttcctg ataaggagtc actaaaagat taattcctaa
    24601 taataataaa taggggagaa aaaagactag aaaagaagaa ataattgcag tactacccac
    24661 aatagccaga tacttctttt tagaaattcg acacaattgt gctgttagaa aatgactctc
    24721 agtatcctct attatctgac tagaataggg cagtgtacag ataagtgcag ctactaggct
    24781 aatcggtgaa aatcctcgaa tagaagaagc ggcaaaaaaa atcgataaac cttcaatttt
    24841 ataaatacca ttgaaagcaa ggaaatttcc taaactcatg caaagaaggg tcaaaaataa
    24901 agacatataa aatcgaggtg attgaacgac tcgtacaaga ttacaaatga aaaattccat
    24961 cct
//
LOCUS       DAVDWT010000030        24766 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVDWT010000030 DAVDWT010000000
VERSION     DAVDWT010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24766
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.030"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(<1..137)
                     /locus_tag="VUW50_001523"
     CDS             complement(<1..137)
                     /locus_tag="VUW50_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7983399.1"
                     /translation="MNETKKYLVIKAIAQGKKTKKRACVELNLSERQINRLLLAYQQK
                     G"
     gene            307..378
                     /locus_tag="VUW50_001524"
     tRNA            307..378
                     /locus_tag="VUW50_001524"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:340..342,aa:Glu,seq:ttc)
     gene            384..457
                     /locus_tag="VUW50_001525"
     tRNA            384..457
                     /locus_tag="VUW50_001525"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:418..420,aa:Asn,seq:gtt)
     gene            complement(498..1040)
                     /locus_tag="VUW50_001526"
     CDS             complement(498..1040)
                     /locus_tag="VUW50_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET7983400.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(1300..2553)
                     /locus_tag="VUW50_001527"
     CDS             complement(1300..2553)
                     /locus_tag="VUW50_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET7983401.1"
                     /translation="MKCFVGLDISSTKLDVCIMLSDTSTPVTASLSNDLSGATEIKNH
                     ILDLNHTYHFERIVIGMEATSLYSFHPAMFFHEDRQLKELHVEIMVEQPNKIKKYRDA
                     FEENKNDTIDAFYIADYFRIERFSPSFLKEEKYMALQHLTRTRLQIIEQLTRTKQHFI
                     ENIYYKCNTLAYQFKKEELSTNLWSSTMMTLMTDEMTLDELANMPLQELDDWLQKLGK
                     GHFKDSEKLAQTIQAAIRGSYRLSKLQQDSVNVILGLLAREIRNLEKMIKEIDKAIED
                     MVETIPEYQCLTSIPGVGKVYAAGIIAEIGQIERFKDHPQVAKFAGLNWREKQSGNSN
                     SQHTSLVNRGNRYLRYYLVEAANSVRRYDDEYKDFYKKKYHEVPKHQHKRAILLTARK
                     FVRLVDVLLRNHQLYTPPRRIMEDN"
     gene            2909..4667
                     /locus_tag="VUW50_001528"
                     /pseudo
     CDS             2909..4667
                     /locus_tag="VUW50_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
     gene            complement(4697..7240)
                     /locus_tag="VUW50_001529"
     CDS             complement(4697..7240)
                     /locus_tag="VUW50_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HET7983402.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLSPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYTLMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYVLFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSTYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVDFDY
                     FSPIPYEKIENTNNLISVTSSSNEDAAIQYQIEVPENSQIYLSFINLHFSNDKQKKVD
                     ILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTKTFT
                     EAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQAQT
                     GFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(7302..8924)
                     /locus_tag="VUW50_001530"
     CDS             complement(7302..8924)
                     /locus_tag="VUW50_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7983403.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            9130..10155
                     /gene="trpS"
                     /locus_tag="VUW50_001531"
     CDS             9130..10155
                     /gene="trpS"
                     /locus_tag="VUW50_001531"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET7983404.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            10307..11785
                     /gene="guaB"
                     /locus_tag="VUW50_001532"
     CDS             10307..11785
                     /gene="guaB"
                     /locus_tag="VUW50_001532"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET7983405.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(11836..12933)
                     /gene="recF"
                     /locus_tag="VUW50_001533"
     CDS             complement(11836..12933)
                     /gene="recF"
                     /locus_tag="VUW50_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET7983406.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSKSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNEN"
     gene            complement(12936..13304)
                     /gene="yaaA"
                     /locus_tag="VUW50_001534"
     CDS             complement(12936..13304)
                     /gene="yaaA"
                     /locus_tag="VUW50_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HET7983407.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKIDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            13463..14713
                     /locus_tag="VUW50_001535"
     CDS             13463..14713
                     /locus_tag="VUW50_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET7983408.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERFQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            14710..15993
                     /locus_tag="VUW50_001536"
     CDS             14710..15993
                     /locus_tag="VUW50_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET7983409.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSIDTLVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLYLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            16035..16856
                     /locus_tag="VUW50_001537"
     CDS             16035..16856
                     /locus_tag="VUW50_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7983410.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            16867..17412
                     /gene="pgsA"
                     /locus_tag="VUW50_001538"
     CDS             16867..17412
                     /gene="pgsA"
                     /locus_tag="VUW50_001538"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET7983411.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            17409..18236
                     /locus_tag="VUW50_001539"
     CDS             17409..18236
                     /locus_tag="VUW50_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET7983412.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            18221..19060
                     /locus_tag="VUW50_001540"
     CDS             18221..19060
                     /locus_tag="VUW50_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET7983413.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRIFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPSILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            19053..19847
                     /locus_tag="VUW50_001541"
     CDS             19053..19847
                     /locus_tag="VUW50_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET7983414.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            19906..20724
                     /gene="mreC"
                     /locus_tag="VUW50_001542"
     CDS             19906..20724
                     /gene="mreC"
                     /locus_tag="VUW50_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET7983415.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            20724..21218
                     /gene="mreD"
                     /locus_tag="VUW50_001543"
     CDS             20724..21218
                     /gene="mreD"
                     /locus_tag="VUW50_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET7983416.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            21312..22490
                     /locus_tag="VUW50_001544"
     CDS             21312..22490
                     /locus_tag="VUW50_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET7983417.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            22714..23493
                     /gene="rpsB"
                     /locus_tag="VUW50_001545"
     CDS             22714..23493
                     /gene="rpsB"
                     /locus_tag="VUW50_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET7983418.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAALETQADSIEEIVEVVEGDNA"
     gene            23572..24612
                     /locus_tag="VUW50_001546"
     CDS             23572..24612
                     /locus_tag="VUW50_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HET7983419.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKATNDFEA
                     EVAATMAAALNN"
BASE COUNT         7820 a         4014 c         5074 g         7858 t
ORIGIN      
        1 tttcctttct gttgataggc tagtaacaga cgattgattt gtctttcaga aagattgagt
       61 tcgacacagg cccgtttctt tgttttcttt ccttgggcta tagcttttat cacaagatat
      121 tttttcgttt cattcatatt cagttggatc cttttcatat gactattcta ccaaatggga
      181 cattttcacg ttcgatttac taaagacatt atcacattcg aattacatat tttagagaaa
      241 aaatctcaaa agtgattgac aattagcaag aaattgatat aatggttata tctgctacag
      301 atagaaggtc cgttggtcaa ggggttaaga caccgccttt tcacggcggt aacacgggtt
      361 cgaatcccgt acggactatt ttatccgcca tagctcagtt ggtagtagcg catgactgtt
      421 aatcatgatg tcgtaggttc gagtcctact ggcggagtat agataaaagg gaacacagct
      481 gtgttcctct ttttgtatca atttgtatca ccaagcattt tcataaggaa gtctgttatt
      541 tcttgagaac tttctttttt tccatgtgca atccaagttt ggcagacacc aaaaagtgca
      601 tgagttagat agatgctact atattctaat tcagtggtat ttagattcag ttgcataaat
      661 cgcttttgta aatctgtact aagcatgata tgaagtttat ttcgtaagaa attttggatt
      721 tctttagtcc cattttcaga aagaagggca gccagaagtg gttctgactc tagatattca
      781 aaaacttcta aaatagcgtc tcttttgtga tgagcatgtt tttgaaaaat atattcaaat
      841 gtatggaata gcttgctttg atagtgctca atcatatcat acttatcctt atagtgagta
      901 tagaagctgg aacgactaat tccggctttt tctactaatt tgacagtaga aattttatca
      961 aatggctgtt ccatcagtaa ttgtaccata gcgttttcaa tagttcgctt tgtttttaag
     1021 cgtttgttac tttcttgcat atttcctcct tgtaaacaaa ttagactata tgtctaaaaa
     1081 tagatttttt atcttgtaat ttagattttt taatgtataa tctattatat caaaatttta
     1141 gacaatatgt ttaaaaaagt ctagtggtaa aatgtcaagc gaccccccgc tcaaagtttg
     1201 aaaactattt caaaaaaagg gtgcactaaa gaagccctac tgaaaacgat gttttcacac
     1261 tgtttttagt agattttcaa ggagaggtgc agatataact tagttatctt ccataatcct
     1321 ccttggtggc gtatagagtt gatggttgcg tagtagcaca tccaccagac gcacaaattt
     1381 tctagcggtt agtaagatgg ctcgtttgtg ttggtgttta ggtacttcat ggtatttctt
     1441 cttatagaaa tccttgtatt cgtcatcata acgccttacc gagttggcgg cttcaaccag
     1501 gtagtagcgc agataacgat tgcccctatt cacaagcgaa gtatgttgtg agtttgagtt
     1561 accagattgc ttttctctcc aattcagacc agcaaatttt gcgacctggg gatggtcttt
     1621 aaagcgttcg atttgtccaa tttcggcaat gataccagca gcgtagacct tcccgacccc
     1681 aggtatggag gtcaggcatt gatattcagg aatggtttcc accatatctt caatggcctt
     1741 atcaatttcc ttgatcattt tttccaggtt tcgaatttcc cgagccagaa gtccgagaat
     1801 gacattgact gaatcttgtt ggagtttaga gaggcgataa gaacctctga tagcggcttg
     1861 aatggtttga gcaagcttct cagagtcctt aaaatgccct ttacccagtt tttggagcca
     1921 atcatctaat tcttgaagag gcatgttagc aagttcgtct aaggtcatct catctgtcat
     1981 caaggtcatc atcgttgaac tccataggtt agtagagagt tcttctttct tgaattgata
     2041 agccaaggta ttgcacttgt agtagatatt ttcaatgaag tgctgtttgg ttctggttaa
     2101 ttgctcgatg atttggagtc tggttcttgt gagatgttgg agagccatgt atttctcttc
     2161 ttttaaaaaa gaaggtgaaa atcgctcaat acggaaataa tccgcaatgt agaaagcatc
     2221 aatggtatcg tttttgtttt cttcgaaagc atcccgatac ttcttgattt tattaggttg
     2281 ttcgaccata atctcaacat gtagttcttt caactgccta tcttcatgga agaacatagc
     2341 agggtggaaa ctgtagagac tggttgcttc catgccaatg acaatccttt cgaagtgata
     2401 ggtatgattc aaatcaagga tatgattctt aatctctgtt gccccagaaa ggtcattaga
     2461 gagggaagca gtgacggggg tgctagtatc actcaacatg atacacacat ctaattttgt
     2521 agagctgatg tctaaaccga caaaacattt cattaaggaa ggtctccttt ccattagatt
     2581 tggaagtttt tttgactact gtaaggtttc ccagaaacac cttgttccaa catcctcgca
     2641 taagagaatc cttatcacag cctgacagag ccgcctgcat cctactagat acagaagtca
     2701 agctgtggaa acagctagtg tgttggaagt ttgaacaagg cttgggtaac agacttttta
     2761 atgaagtcca agcaacaagg agaaaaagaa acaaccatga gtccattcca tgactctatt
     2821 gttctagaaa accttgcaat agtcaagtga taacgaataa aacttgggat tgaaatatcc
     2881 ccatgattat atattacgag gagaaactat gtttaaagaa tggaaagcaa tttaaaaaaa
     2941 acaaccttta ttattgtcat gatcgggatt tctcttattc cagctctgta caatatcata
     3001 tttttgtcat caatgtggga tccatatggg caattgtctg acttacctgt ggcagttgta
     3061 aataatgata aagaggcttc ctataatggt aatactatgg caataggaaa agacatggtg
     3121 tccaatttaa aagaaaataa aaccttggat tttcattttg tagatgaaga ggaaggaaag
     3181 aagggattgg aagatggcga ttactatatg gtagtgactt taccaagtga tttatctgaa
     3241 aaagcaacta cattatccaa tattcaatcg acagcagctt atcaatcatt gacaagtgag
     3301 caacaaactg agataagtga ttctgtatct caaaattcaa ctgatagtat tcaatcggct
     3361 cagtcaattg tagctttagt acaagattta cagggaagtt tagaaaactt acaaaatcaa
     3421 tcttctaatc tttcgacttt aaaaaatcaa gctaatcaag tattacctat tacttctact
     3481 tctttgatag gattgtcaag tggattaaca gagatacaag gagctgttac tagcaaatta
     3541 gttcctgcca gtcagtcgat tgcatcaggt gtaaacgcat atactacagg tgttgataaa
     3601 gtttctcagg gcgcaagtca actaagtgaa aaaaatgcta ccttgacagg tagtttggat
     3661 caattagttt caggctcaaa caccttgaca caaaaatctt ctagcttgac agcaggagtt
     3721 ggtcaattag ttgaaaaaac tccagaatta gtatctggta ttgaaaaatt atcaactggc
     3781 tccaattaat tgaatcaaaa gagtcaagaa ttgatagcag gagttgataa attgcagtca
     3841 ggctctagcc aactagctga caaatccagt cagttacttt caggtgtttc tcaattagaa
     3901 agtggagcta ataaattggc agatggagct gggaaactag cagaaggtgg aacaaagtta
     3961 acttctggat tggaaggttt acagacagga cttgcttctt taggacaagg actaggtaat
     4021 gctagtgatc aactcaaatc agcatcaaca gaatctaaaa atgcagagat tttgtcaaat
     4081 ccactcaatc tttccaaaac agacaatgac caagttcctg taaatggaat tgcaatggct
     4141 ccttatatga tatcagttgc tctttttgtt gcagcaatat caacgaatat gatctttgcg
     4201 aagttacctt caggacgtca tccagagagc cgttgggctt ggttgaaatc tcgagctgaa
     4261 ataaatggta ttatagctgt tttggcaggg attttggtat atggaggagt tcatcttatt
     4321 ggtctaactg ctaatcatga aatgagaaca tttattctca tcatcttaac aagtttagta
     4381 ttcatgtcta tggtgaccgc tttaacaaca tggaatagcc gtataggagc tttcttctcg
     4441 cttatttttc ttttattaca gttagcatca agtgcaggta cctatccgct tgctttgaca
     4501 aatgatttct ttagagctat taatccttgg ttgccaatga gttattcagt ttcgggatta
     4561 cgacaaacaa tctccatcaa caagtcattt tcctagctgt catactagct ctatttactg
     4621 gtttaggtat gctagcctat caacctaaga aaatggaaga agattaaaaa aatcgaccga
     4681 ttaactggtc gattttttat gccttagatg actttcgtct gtgattatag attccaaata
     4741 gtaagagaga agtaaaggaa cagattgctc cagtaataaa accattggga atgaaggaaa
     4801 gtgtaatagt tcctttcccc ttgggaatgt caactttcat aaatccagtt tgagcttgtt
     4861 taatttctat tttcttacca tcttggtagg cagaccaacc tttgtcataa ggaatggtga
     4921 agaaaataga tgtatcttgt tggacatcat atgtagcaaa aaccttgttt ttagaagttg
     4981 atactgtgac aggttgttct ttaatttttt gaattgcctc ggtgaaagtt ttggtatcta
     5041 aacgatagaa ggtaggagat tcaaatgata cttgtgaatt tccagggaaa ctaacattga
     5101 tattgaaagt ttttttctct ttagtatatc ctagattaaa gaaggagaag acattatcag
     5161 ttgtaaaagt ctttttttca ccatttacaa ggatgtcaac cttcttttgt ttatcgttag
     5221 aaaagtgaag gtttatgaaa gagagataaa tttggctgtt ttctggaact tcaatttgat
     5281 actggattgc tgcatcctca tttgaagaac ttgtgacact aatcaaatta ttagtatttt
     5341 ctattttttc ataagggatt ggagaaaaat aatcaaaatc aacgttagca agttgattta
     5401 aaaacgaggc ctgattatcc aaggtatgtt cattgaactt gacatcattg taaacagatt
     5461 gactagcaac tgcaatcgga agagagtatt gattttcata tagggtaaga ttatcttttt
     5521 gatagatatc tttaaaacca tacttatcaa taggactgtc tgagatattg tactggatac
     5581 caaataaact atcagccaaa atactattat ttgcatatcg gagattgaga ttagtcccag
     5641 aggatttaaa cccaagttta tctaaagtag agcttgatga acgatttcga acagatgaaa
     5701 attgagagat tccattgtag ttgaatttca tactgtcatt tcctgtctga gtttgtagtt
     5761 tttcagtacg agtaaattga tttccaatat atgttgagaa agattccata gctgggatat
     5821 ctcgactata agtacttcga gaagcaaatc cccattcctt agcaattccg tccatttgag
     5881 atgaagcatt taaactcatt tcaaccagta taaataaaga gattagaatg gcaaatagat
     5941 tcacagatat aaactttttg ataactgcaa ggagtaaaag agaatagaca accaaaaatt
     6001 caagagtaag cagaatattc aaatctgtta aaaaagaata atgcgatttt agatagatgg
     6061 tagctaaaaa tcctgttact acaagaaaaa gcgaaactaa aaaattccag actttaagtt
     6121 ctttcagacg ctttaagact tctgctgctg tgtaaattaa caaggtagag aaaatccaag
     6181 catagcgatg taaaaacatg tttggagtat gcatgccttg ccaaaataag tcaagagctt
     6241 ctatgtaaaa gcttgcaatt agaaatgcaa agaataatac atatatgagt ttcacgtgaa
     6301 acttaataga tttcagcgta aaaaataaaa tggtcaaaat aaaaggaagt agtccaacaa
     6361 aaatcattgg gatggcccca tactttgttg tgtcaaagga accaatgaat tgcttagcaa
     6421 agagatcaag ataccagcta ctttcagttt gaaactttgt aacttcagtc aatttttccc
     6481 catgtgtctg taaatcaaac agagtgggaa gagtcataat caaactagcc ataccagcta
     6541 aaaaggagat aactatgaaa tcaagaacag atgattttcg agtcttaaag tcccacgaaa
     6601 tttgacagag ataccagaaa ataagaaaca atactgtcat atatccaaaa taataatttt
     6661 gaataaataa gattgacaga cttgtaaagt acaataggag tttcttttca gttatcagta
     6721 gatgtaaacc agttataatt aaaggaatca agataaaaac atctagccag gtttttatct
     6781 ctaattgact gacagtgaaa ctcatcagag tataggaagt agataaggct agttttaaag
     6841 tctgagggat agattgaaac aatttattca aactaaaaaa ggttgacaga ccaatcaatc
     6901 caaattttaa gagagtggtc agataaacag catctggcat attcgttaga tcaaaaaagt
     6961 aaaccagagg tgagagaaaa ctacccaagt aataactaga cagagcatag aagtttagcc
     7021 ctagaccact tgtaaaggtg taaaacagac tactatttcc atgtaggata tttcgtaagg
     7081 ctacatcaaa aataacgtat tgatgaaagc catctcctaa tagaggagag ttgtcgctat
     7141 tccagtagat accttgagat agatatactc cagacataat cactacagga atgatgaaag
     7201 aaataaaata ggttcgatat gtttttaaaa atgatttcat gttacctcgt agaatgatag
     7261 aaaactcagt tggttaaccc aactgagttt tgaagtttta tttagtcttt ccaaagttct
     7321 ttaacttttg cttgtacttc tgcattttct aggaattcat cgtaggtttc atcgatacgg
     7381 tcaatgacgc catttttaga taagacaatg atatggttag ccaaagtttg aataaattcg
     7441 tggtcatggc tggcaaagat gattgattct ttaaagtttt tcaatccatc attcaagctt
     7501 gagatagatt ccaagtccaa gtgatttgtt ggatcatcaa gtacaaggac atttgatttt
     7561 aagagcatga gttttgaaag catgacacga actttttctc cccctgacaa gacatttaca
     7621 ggtttgttaa cttcatctcc agagaagagc atacggccga ggaagccacg taggaaagta
     7681 ttgtcatctt cttctttact tgcgaattga cgcaaccagt caagaattga ttctcctcct
     7741 gcaaaatcag ctgagttatc ttttggtagg taagattgac tagttgtaac tccccacttg
     7801 acagttcctt catagtcaat atctcccatg attgcacgaa ttaatgcagt tgtttgaata
     7861 tcattttgtc caataagtgc tgtcttatca tctggacgca agatgaaact aatattatcc
     7921 aagatagttt caccatcaat ctttacagtt agattttcta ctgtcaagag atcattacca
     7981 atctcacgtt ccgctttaaa gttgataaat ggatatttac gactagatgg tacaatctct
     8041 tctagctcaa tcttatcaag cattttctta cgtgatgttg cctgccttga cttagaagca
     8101 ttggcagaga aacgagcaac gaattcttgc aattgtttaa ttttttcttc tgctttagca
     8161 ttacggtctg ctagcaattt agcagcaagc tcagaagatt ccttccagaa gtcgtagttt
     8221 ccgacataga gtttgatttt tccaaagtca aggtcggcca tgtgagtaca aactttgttt
     8281 aagaagtgac ggtcgtggga tactacgata actgtgttat caaagtcaat taagaagtct
     8341 tctaaccaag taatcgattg gatatccaaa ccgttagtag gctcgtccaa gagaagaaca
     8401 tctggtttac caaaaagtgc tttggcgagg agaaccttta ctttttcacc gttggccaat
     8461 tcgctcatgt tttggtagtg taattcttct ggaatgttta ggttttgaag tagttgagag
     8521 gcttcactct ctgcttccca acctccaagt tcggcaaact ctccttcgag ttcggcagca
     8581 cgaaccccgt cctcgtctga gaaatcttcc ttcatgtaga tagcatcttt ctctttcatg
     8641 atgctataaa gtttttcatt tcccatgata acgacatcaa tggcacgttc atcttcgtag
     8701 tcaaagtgat tttgacgaag aacagagaga cgttcatctg gaccaagaga gatgtgacca
     8761 gtagtaggtt cgatatctcc agctaaaatt tttaaaaagg ttgattttcc ggcaccatta
     8821 gcaccgatta atccgtaagt atttccttct gtaaatttga tattgacatc atcaaaaagt
     8881 ttgcgatcac taaaacgtag tgaaacatca gatactgtaa gcaatatttt tctcctatat
     8941 gtgtaatata tttattctac tagaaaatac agaaatattc aaatttttat ttgtcaattt
     9001 tgtgtaaatt atatttacag tatcctttac acaaatctgt aaaaagcaag gctgatttat
     9061 tttgataaat tacggttatt tcattaaaaa aatgctataa ttgaaaggac tatatcgaag
     9121 gagaacaaaa tgactaaacc cattatttta acaggagacc gtccaacagg aaaattgcat
     9181 attggacatt atgttggaag tctcaaaaat cgagtattat tacaggaaga ggataagtat
     9241 gatatgtttg tgttcttggc tgaccaacaa gccttgacag atcatgccaa agatcctcaa
     9301 accattgtag agtctatcgg aaatgtggct ttggattatc ttgcagttgg attggatcca
     9361 aataagtcaa ctatttttat tcaaagccag attccagagt tggctgagtt gtctatgtat
     9421 tatatgaatc tagtttcgtt agcacgtttg gagcgaaatc caacagtcaa gacagagatt
     9481 tctcagaaag gatttggaga aagcattccg acaggattct tggtctatcc aatcgctcaa
     9541 gcagctgata tcacagcttt caaggctaat tatgttcctg ttgggacaga tcagaaacca
     9601 atgattgagc aaactcgtga aattgttcgt tcttttaaca atgcatataa ctgtgatgtc
     9661 ttggtagagc cggaaggtat ttatccagaa aatgagagag cagggcgttt gcctggttta
     9721 gatggaaatg ctaaaatgtc taaatcacta aataatggta tttatttagc tgatgatgcg
     9781 gatactttgc gtaaaaaagt aatgagtatg tatacagatc cagatcatat ccgcgttgag
     9841 gatccaggta agattgaggg aaatatggtt ttccattatc tagatgtttt tggtcgtcca
     9901 gaagatgctc aagaaattgc tgatatgaaa gaacgttatc aacgaggtgg tcttggtgat
     9961 gtgaagacca agcgttatct acttgaaata ttagaacgtg aactgggtcc tattcgtgag
    10021 cgccgtattg aatttgctaa ggatatggga gaagtttata atatgattca aaaaggtagt
    10081 gaaagagcgc gtgaagttgc gggtcaaacc ctatctgagg taaaaggggc aatgggactt
    10141 cattacttta actaagttta ttttacaaga aactatcttt tctatctcga agaaaagata
    10201 gttttttatt tacccctgta acatttgaca aatttttata gaatggtatg atagatacaa
    10261 tataaaaaga gtcaagctca aaaagaaaga aaagaggaaa cttcgaatgt ctaattggga
    10321 cactaaattt ttgaaaaaag gttttacctt tgatgatgta ttgcttattc cagctgaaag
    10381 tcatgtgttg cctaacgatg cagatttaac aactaaattg gcagataatt tgactttaaa
    10441 tatcccaatt attaccgctg ccatggacac agttacagag agtcaaatgg ccattgctat
    10501 tgctcgtgca ggtggtctcg gagttatcca taaaaacatg tcaattgctc aacaagcaga
    10561 cgaggttcgt aaggtaaaac gttctgaaaa tggagttatt attgatccgt tcttcttgac
    10621 gcctgaacat acaattgctg aagcagatga gcttatgggt cgttaccgca tcagtggtgt
    10681 tccagttgtt gaaacacttg aaaatcgtaa attggttggt attttgacaa accgagatct
    10741 tcgttttatt tcagattata atcaaccaat ttcaaaccat atgactagtg aaaatcttgt
    10801 tactgctcct gtgggtacgg atcttgcaac ggctgagagt attcttcaag agcatcgtat
    10861 tgaaaaactt ccgttggtcg atgaagaagg cagtctttct ggtttgatca ctatcaaaga
    10921 tattgaaaaa gttattgagt ttccaaatgc ggctaaagat gagtttggtc gtcttctagt
    10981 tgcaggtgca gtaggtgtta cttcagatac atttgaacgt gcagaggctc tttttgaggc
    11041 aggagcggat gcgattgtta ttgatactgc acatggtcat tctgcaggtg tcttgcgtaa
    11101 aattgccgag attcgtgctc atttcccaga tcggactttg attgctggaa atattgctac
    11161 tgctgaaggt gcacgtgccc tttatgaagc gggtgtagac gttgttaagg ttggtattgg
    11221 accaggttct atctgtacta ctcgtgtgat tgctggtgtt ggtgttccgc aagtaacagc
    11281 tatctacgat gctgcagctg ttgcgcgcga atatggtaaa acgattattg ctgacggtgg
    11341 gatcaagtat tctggagata ttgtaaaagc acttgctgca ggtggaaatg ctgttatgct
    11401 tggatctatg tttgctggaa ctgatgaagc tccaggcgaa actgaaatct tccaaggacg
    11461 taaattcaag acttaccgtg gtatgggatc aattgctgct atgaagaaag gttcaagcga
    11521 ccgttatttc caaggttctg tcaatgaagc aaacaagctt gttccagaag gaattgaagg
    11581 tcgtgttgct tataaaggag cggcagctga tattgttttc caaatgattg gtggtattcg
    11641 ctctggtatg ggttactgtg gtgcagctaa ccttaaagaa ctacacgata atgctcaatt
    11701 tattgaaatg tctggtgctg gtttgaaaga aagccatcct catgatgtgc aaattactaa
    11761 tgaggcacca aattattcta tgtaaaaaac aatgaaaaga actccagtga aaacaggagt
    11821 tcttttacaa tgttgtcaat tttcatttac agcagcttta ccatcctgaa tagtgaagat
    11881 acttagattt tctggcagat tttgaagatg gtctaagctt gttgttgtga taaaggtttg
    11941 gattgattga gaaatcgttt ctaataattt taattgtcta gtgttgtcaa gttcactcat
    12001 cacatcgtca agcagtaata taggagattc tgtggtaatg ctttccatta attcgatttc
    12061 tgctaatttt atcgagagga cgagactacg atgttgacct tggcttccga aactagcatc
    12121 catcccattt atataaaaag aaatgtcatc tcgatgagga ccgacaccag tattcttttt
    12181 aaataaatct ctggatttac ttttttctaa agcaattttg aaagattctg ataagttttg
    12241 tttgtcagtt atattgacag aagattgata ggatattgac aactcttcga tctgattaga
    12301 gagttcaaaa tgtttcttac gcccaaatga ttctagtttt tttatgaaat ctaagcggtg
    12361 attcattaca cgacatccat aatcaactag ctgatcatct aacacagaaa ggaatgtttc
    12421 atctattttt tgagctgatt ttaggtaagt gtttctttgc tttaggatgt ggttataatt
    12481 ggttaagtca gataaataga ttggcttaat ttgcccaagt tccatatcaa tgaattttcg
    12541 tcgaatcgaa ggtgctcctt taattagttg taaatcttca ggagcaaata agacaacatt
    12601 catgtgtcct acataatctg aaaggcgtgc ctgttttaag tgattaactt ttgttacacg
    12661 gcctttttgt gttagttcga tttctagggg aatggatcca gtttttttct gaacgagacc
    12721 tgaaagatga agttgttcct catcaaaatg aatgagattt ttatctgttc gagttcgatg
    12781 actacgcgtt aaggctaaaa aatagatagc ctctaacatg tttgtttttc cttgtgcatt
    12841 acgtcccaaa aagacattca atttaggatt aaagtctatt ttcgtttctt tgtaattacg
    12901 aaaagtcttg agagatagat gttgtagcca catgattatc taccagggaa tcgtggagct
    12961 tgttggcttt taggtgatga agcaggtttc gatttgtctt ttttaactcc cttattcatc
    13021 tttttgacaa gtttagcgat ccgttctttt tcaactttat cagcttggta ttcatcttgt
    13081 tcttcagaag taggttgtgt caacaagatg tcaatgttca tgtcagggat gtcaatttta
    13141 tcgccaatac gaagtttttt accacgacga ctctctaatt ccctattaaa gtaaacagaa
    13201 tgttcagaga gaaatgattt aatagctcct ccgctatgtg taattccaag ttctttgagt
    13261 agtgcttgga gggtaataaa ttcttcaaat aatttgtatt ccataattca cctcaatctt
    13321 tggtattata ccatattttc ctaaaaatag tgagagagac agcgagaaat gtaagcgatt
    13381 tttatttcaa aagtgttaca gggaacaaga aaatttcagg ttttcgtgat ataatagaag
    13441 tctgtatata aggaggtaaa tcatggagtt agtgcatgga atttcaacac attttatcca
    13501 atcaaaaaag tttaaaacaa acaaaattac cgtgcgtttt accgctccat tatcccttga
    13561 tacgattgca ggtcacatgt tgagtgcaag tatgctagag actgctaatc agatgtaccc
    13621 cacttctcaa gatttgagga gacacttggc cagtctatac ggtacagata tgtcaaccaa
    13681 ttgtttcaga agagggcaaa gccatattat agaattgaca tttacctatg ttcgtgatga
    13741 gtttttaagt aggaaaaacg tgctaacctc tcagattttg gaacttgtaa aagaaactct
    13801 tttttcaccc gtagtagttg ataatgggtt tgatccggcc ttatttgaaa ttgagaaaaa
    13861 acaattgcta gcaagtttag cagctgatat ggatgattct ttttattttg cacataaaga
    13921 attggataaa ttgttttttc atgatgaacg ttttcaattg gaatatagtg atttacgaaa
    13981 tcgtatttta gctgaaactc cacaaagttc ttattcttgt ttccaagaat ttttagccaa
    14041 tgatcgaata gatttctttt tcctaggtga ttttaatgag gttgaaattc aaaatgtatt
    14101 agaatcattt ggctttaaag gtcgaaaagg agatgtgaag gttcagtatt gtcaacctta
    14161 ttctaatatc cttcaggaag gtatggttcg gaaaaatgtg ggacaatcca ttttggaatt
    14221 aggttatcat taccgttcta aatatggtga tgagcaacat ttacccatga ttgtaatgaa
    14281 tggtttactt ggtggatttg ctcactctaa gctctttaca aatgtccgtg aaaatgctgg
    14341 attagcttat accatttcaa gtgagcttga tttatttagt ggattcttga ggatgtatgc
    14401 tggtatcaat cgagaaaatc gtaaccaggc tcgtaaaatg atgaataatc aactgcttga
    14461 tttaaaaaaa ggttatttta cagagtttga gttaaatcag accaaggaaa tgattcgttg
    14521 gtcgttgtta ctttctcaag ataatcaatc ttcattgatt gaacgtgctt atcaaaatgc
    14581 cttatttgga aaatcttcag cagactttaa aagttggatt gcaaagcttg aacaaattga
    14641 caaagatgct atttgtagag tagctaataa tgtgaaacta caagcgattt actttatgga
    14701 aggaatagaa tgacaaaggt tgtttttgaa gaaaaatact atccagctgt aaaagaaatg
    14761 gtttatcgaa ctcgtttggc caacggattg acagttgctc ttttgcctaa aaaggaattt
    14821 aaagaggttt acgggagtgt aactgtacag tttggttcga tagatacgct tgtcacagaa
    14881 gttgacggag atgtaaaaca atatcctgga ggaattgctc attttcttga acataaatta
    14941 tttgagagag aagattctag tgatttgatg tcggctttta cgagtctagg tgcagatagt
    15001 aatgccttta caagctttac aaaaacaaac tatctttttt cagcaacgga ttatttttta
    15061 gaaaatttat atttacttga tgaattggta acatcagcac actttactga agcttccatt
    15121 ctgacagagc aggatattat tcagcaagaa cgagaaatgt accaagatga tccagattcg
    15181 tgtttattct tttcaacttt agcgaatttg tatcctggta cacctttagc aactgatata
    15241 gttggaagtg aggagtccat ttcccaaatc aatctaacta atttgcaaga aaattttaca
    15301 aagttttaca aacctgtaaa catgtctctg tttttagttg gtaattttga tgtggagcga
    15361 gtacaggact attttgaaag caaagaactg aaagattcag attttcagga agtagcaaga
    15421 gaaaagttgt ttttacagcc tgtaaagcca acagatagta tgagaatgga agtatcttct
    15481 cccaaactag cgattggagt tagaggtaag cgagaagttt ctgaagcgga ttgctatcga
    15541 catcatattt tattaaagtt attgtttgca atgatgtttg gttggacttc ggatcgtttt
    15601 caaaaatgtt atgaatcagg taaaattgat gcatccttat ctctggaagt tgaagtaaca
    15661 agtcgctttc attttgtcat gttgacaatg gatacgaaag agccagttgc tttgtctcat
    15721 caatttagga aggctattcg taattttaca aaggatttag atattacaga ggaacattta
    15781 gatattatca aaagagagat gtttggcgaa tttttcagta gcatgaactc tcttgaattt
    15841 attgcaacgc aatatgatgc ttttgaaaat ggtgagacaa tttttgattt gccgaaaatt
    15901 ttacaggaaa ttactttaga ggatgtcctt gatgctggac atcatttaat agatgatggt
    15961 gacatagttg attttacaat attcccatcg tagtaaccta ttataataga cactagaaag
    16021 aagggatgac aagtatgaga aaaaaaacaa ttggagaggt tttacgatta gctagaatca
    16081 atcagggatt gagtttagat gaattgcaga aaaagacaga aatccagtta gatatgttgg
    16141 aagcaatgga agcagacgat ttcgatcaac ttccaagtcc tttttacacg cgttctttct
    16201 tgaaaaaata tgcatgggct gttgagttag atgaccaaat tgttttggat gcttatgatt
    16261 ctgggagtat gattacttat gaggaagtag atgttgatga agatgagttg acaggtcgta
    16321 gacgttcaag taagaaaaag aagaaaaaaa catcattttt acctttattt tattttatcc
    16381 tttttgcttt atcgatttta atttttgtga cttattatgt ttggaactat attcaaactc
    16441 aaccagagga gccttctctt tctaattaca gtgtggttca atcaacaagt tcaactagct
    16501 ctgttcccca ctcctcaagt agtagttctt ctagtataga atcagctata agtgtatcag
    16561 gcgaaggaaa tcatgtagaa atcgcttata agacaagtaa ggaaacagtt aaattgcaat
    16621 tggcagtttc agatgttaca agttgggtca gtgtttcaga aagcgaactt gagggcggtg
    16681 taaccttatc gccaaagaag aaaagtgcag aagcaacagt tgcaactaaa agtcctgtaa
    16741 caattacgtt aggtgttgta aaaggtgttg atttgacagt agataatcag actgttgatt
    16801 tatcgaaatt aacagctcag actggacaaa tcactgtaac ctttactaaa aattaaggaa
    16861 aaacgaatga aaaaagaaca aattcccaat ctcttaacaa taggtcgaat tctctttata
    16921 cctattttta tctttatttt aacgatagga aattcgatag agagtcatat agttgcagct
    16981 attatctttg ctgttgccag tattaccgac tatttagatg gatatttagc tcgtaaatgg
    17041 aatgtggtca gtaattttgg taaatttgca gatcctatgg cggataagtt actagttatg
    17101 tcggctttta ttatgttgat tgagttaggt atggctccgg cttggattgt tgcagtgatt
    17161 atctgtcgtg agttagctgt gacaggttta aggcttttat tggttgaaac tggtggaaca
    17221 attttagcag cagcaatgcc tggaaaaatt aaaactttta gtcagatgtt tgccattatt
    17281 ttcttgctat tacattggac tttgcttggt caagttctac tttatgtagc cttatttttc
    17341 actatctact ctggctatga ctatttcaag ggtagtgcct atgtatttaa agggacattt
    17401 ggttcgaaat gaaatcaata attgatgtaa aaaatctttc ttttcgctat aaagaaaatc
    17461 agaactacta cgatgtgaag gatattacgt ttcacgtgaa acgtggagaa tggctttcga
    17521 ttgtagggca taatggtagt ggtaaatcaa cgacggttcg attaattgat ggcttactgg
    17581 aagcagaatc cggagagatt gtaattgatg gccaacgttt gactgaggaa aatgtttgga
    17641 atatacgtcg tcaaatcggt atggtttttc aaaatccaga caatcaattt gttggagcga
    17701 ctgttgaaga tgatgttgct tttggtttgg aaaatcaggg actttctcgt caagaaatga
    17761 aaaagagagt agaagaagct ctggctttag ttggcatgtt ggactttaaa aagagagagc
    17821 cagcgcgtct atcaggtggc caaaagcaac gtgtggccat tgcaggtgtt gtagccctaa
    17881 gaccagctat tttaatctta gatgaagcaa cgagtatgtt ggatcctgag gggcgtagag
    17941 aacttattgg gacagtaaaa ggaattcgaa aagactatga tatgacggtc atttctatta
    18001 cccatgattt ggaagaagtc gccatgagtg atcgcgtatt ggtcatgaaa aaaggggaaa
    18061 ttgaatcaac tagtagtcca agggagcttt tctctcgaaa tgatttagat caaattggat
    18121 tagacgatcc ttttgctaat caattaaaaa aatctttgag ccagaatggc tatgatttac
    18181 ctgaaaatta tttgacagaa agtgagctag aggataagct atgggaattg ctctagaaaa
    18241 tgtgaatttt acatatcaag aaggtactcc cttagcttca gcagctttgt cggatgtttc
    18301 tttgacgatt gaagatggct cttatacagc tttaattggg cacacaggta gtggtaaatc
    18361 aactatttta caactcttaa atggtttatt ggtaccaagt caagggagtg tgaggatttt
    18421 tgatacctta atcacctcga cttctaaaaa taaagatatt cgtcaaatta gaaaacaggt
    18481 tggcttggta tttcagtttg ctgaaaatca gatttttgaa gaaacggttt tgaaggacgt
    18541 tgcttttgga ccgcaaaatt ttggagtttc tgaagaagat gctgtgaaga ctgcgcgtga
    18601 gaaactggct ctggttggaa ttgatgaatc actttttgat cgtagtccgt ttgagctgtc
    18661 agggggacaa atgagacgtg ttgccattgc aggcatactt gccatggagc catctatatt
    18721 agtcttagat gagccaacag ctggtctaga tcctctaggg agaaaagagt tgatgaccct
    18781 gttcaaaaaa ctccaccagt cagggatgac catcgtcttg gtaacgcatt tgatggatga
    18841 tgttgctgaa tatgctaatc aagtctatgt aatggaaaag ggacgtttag taaagggggg
    18901 caaaccaagt gatgtctttc aagacgttgt ttttatggaa gaagttcagt tgggagtacc
    18961 taaaattacg gccttttgta aacgattggc tgatagaggc gtgtcattta aacgattacc
    19021 gattaagata gaggagttca aggagtcgct aaatggatag tatgattttg gggcgttata
    19081 tcccagggga ttcgattgtt caccgattgg atccacgtag caaattgctt gctatgatgc
    19141 tactgatttt gattgtgttt tgggctaata accccttgac gaatctgatt ctttttatag
    19201 cgacagggat atttattgcc ttgtcaggag tatctctttc attttttatt cagggcttga
    19261 agtctatgtt tttcttgatt gccttcacaa ctatttttca actatttttc atttctaatg
    19321 ggaatgtttt atttgagttt tcgtttgtga gaatcacgga ttatgctttg caacaagctg
    19381 ggattatttt ttgtcgcttt gtattgatta ttttcttttc aactttgtta accttaacaa
    19441 ccatgccttt aagtttggca tcagctgtcg aagctttatt agcaccttta aagcgtgtga
    19501 aagttccagt tcatgaaatt ggattgatgc tgtctatgag tttgcgtttt gtcccaacct
    19561 tgatggatga tacgacgcgg attatgaatg cacagaaagc tcgtggagtg gattttggag
    19621 aaggaagtat cgttcaaaaa gtaaaggcga tgattcccat tttgattcct ctttttgcga
    19681 caagtttaaa acgtgcagat tccttggcta tcgctatgga agcgcgtggc tatcagggtg
    19741 gaaaaggcag aagtcaatac cgacaattga aatggactct aaaggatacg ctgaccattc
    19801 ttgttattct cgtacttggt tgttgtttat ttttcttaaa atcttagtag ctttcaggaa
    19861 ttgataaaaa gttactgtaa cagtttttga tataaaggta ggaatatgaa ccgttttaaa
    19921 aaatcaaaat atgtcattat tgtttttgtc actgttctgc ttgtgtcagc tctcttagcg
    19981 acgacttatt caagtacaat tgtgacaaaa ttaggagatg gaatctcatt ggttgataga
    20041 gttgtacaaa aaccttttca gtggtttgat tctgtcaaat cagatttggc tcatttgaca
    20101 cgaacatata atgaaaatga aagtttgaag aaacagcttt accaattaga agttaaatca
    20161 aatgaggtgg aaagtttaaa gacagaaaat gaacaactgc gccaattgct tgatatgaag
    20221 tctaaattgc aagccacaaa gactttagca gcagatgtta ttatgcgttc tccggtatct
    20281 tggaagcagg agttgacctt agatgcaggt agatcaaaag gtgcttctga gaacatgtta
    20341 gctattgcaa atggtggctt gattgggagt gtttcaaaag tagaggagaa ctctactata
    20401 gtcaaccttc tgacaaatac ggaaaatgct gataagattt ctgttaaaat tcaacatggc
    20461 tctactacaa tttatggaat tattattggc tatgacaagg aaaatgacgt tcttaaaatt
    20521 agccaattaa atagtaatag cgatattagt gcgggagata aggtgactac tggtggatta
    20581 ggaaacttta acgttgctga tattcctgtt ggtgaagtgg ttgccacaac gcatagtaca
    20641 gactatttga cacgagaagt aactgttaaa ttgagtgcag atactcataa tgtagatgtg
    20701 atagaattag tggggaattc ataatgagac agttgaagcg agttggagta tttttattgc
    20761 ttcctttctt tgttctaatt gacgcccata ttagccagct tctgggctca tttttccccc
    20821 atgtacattt ggctagtcat tttctttttc tatttctctt atttgagacg atagaagtat
    20881 cagagtatct ctacctagtc tattgttttg ttataggctt ggtttatgat gtttactttt
    20941 tccatctaat agggattaca actctcttat ttatcttatt gggagccttc cttcataaat
    21001 tgaatagtgt tattttgttg aatcgttgga caagaatgct agctatgatt gtgctgacat
    21061 tcctgtttga aatgggtagt tatcttttgg cttttatggt agggttgaca gtagatagca
    21121 tgtcgatttt tatagtctat agcttggtac cgacgatgat tttaaatttt ttatggatta
    21181 ctgtttttca atttattttt gaaaaatatt atctataaga acgacatata aatgtaacaa
    21241 aggcgtaata tttattaggc ctttttttgg tatactagta ttgtctttaa aagaaggagt
    21301 atctacgtaa tatgaagaaa aaaatcttag cgtcactttt attaagtaca gtaatggttt
    21361 ctcaagtagc tgttttaaca actgcgcatg cagaaacgac tgatgacaaa attgctgctc
    21421 aagataataa aattagtaac ttaacagcac aacaacaaga agcccaaaaa caagttgacc
    21481 aaattcagga gcaagtatca gctattcaag ctgagcagtc taacttgcaa gctgaaaatg
    21541 atagattaca agcagaatct aagaaactcg agggtgagat tacagaactt tctaaaaaca
    21601 ttgtttctcg taaccaatcg ttggaaaaac aagctcgtag tgctcaaaca aatggagccg
    21661 taactagcta tatcaatacc attgtaaact caaaatcaat tacagaagct atttcacgtg
    21721 ttgctgcaat gagtgaaatc gtatctgcaa acaacaaaat gttagaacaa caaaaggcag
    21781 ataaaaaagc tatttctgaa aaacaagtag caaataatga tgctatcaat actgtaattg
    21841 ctaatcaaca aaaattggct gatgatgctc aagcattgac tacgaaacag gcagaactaa
    21901 aagctgctga attaagtctt gctgctgaga aagcgacagc tgaaggggaa aaagcaagtc
    21961 tattagagca aaaagcagca gctgaggcag aggctcgtgc agctgcggta gcagaagcag
    22021 cttataaaga aaaacgagct agccaacaac aatcagtact tgcttcagca aacactaact
    22081 taacagctca agtgcaagca gtatctgaat ctgcagcagc acctgtccgt gcaaaagttc
    22141 gtccaacata cagtacaaac gcttcaagtt atccaattgg agaatgtaca tggggagtaa
    22201 aaacattggc accttgggct ggagactact ggggtaatgg agcacagtgg gctacaagtg
    22261 cagcagcagc aggtttccgt acaggttcaa cacctcaagt tggagcaatt gcatgttgga
    22321 atgatggtgg atatggtcac gtagcggttg ttacagctgt tgaatcaaca acacgtatcc
    22381 aagtatcaga atcaaattat gcaggtaatc gtacaattgg aaatcaccgt ggatggttca
    22441 atccaacaac aacttctgaa ggttttgtta catatattta tgcagattaa tttacagagg
    22501 gactcgaata gagccctctt ttcaggtttt accgtgacaa tccctattaa aaattatatc
    22561 aaaatagctt gaaaatattg gaaaagtatg gtagaatgaa aattgtcgtg tgaacgataa
    22621 tactcattct tgatgaattg tgaagcagtt gcccttgggt cgttttgcga gttgaagtca
    22681 agaagaggaa aaaaacaaaa aggagaaata ctcatggcag taatttcaat gaaacaactt
    22741 cttgaggctg gtgtacactt tggtcaccaa actcgtcgct ggaatcctaa gatggctaag
    22801 tacatcttta ctgaacgtaa cggaatccac gttatcgact tgcaacaaac tgtaaaatac
    22861 gctgatcaag catacgactt catgcgtgat gcagcagcta acgatgcagt tgtattgttc
    22921 gttggtacta agaaacaagc agctgatgca gttgctgaag aagcagtacg ttcaggtcaa
    22981 tacttcatca accaccgttg gttgggtgga actcttacaa actggggaac aatccaaaaa
    23041 cgtatcgctc gtttgaaaga aattaaacgt atggaagaag atggaacttt cgaagttctt
    23101 cctaagaaag aagttgcact tcttaacaaa caacgtgcgc gtcttgaaaa attcttgggc
    23161 ggtatcgaag atatgcctcg tatcccagat gtgatgtacg tagttgaccc acataaagag
    23221 caaatcgctg ttaaagaagc taaaaaattg ggaatcccag ttgtagcgat ggttgacacc
    23281 aatactgatc cagatgatat cgatgtaatc atcccagcta acgatgacgc tatccgtgct
    23341 gttaaattga tcacagctaa attggctgac gctattatcg aaggacgtca aggtgaggat
    23401 gcagtagcag ttgaagcaga atttgcagct ttagaaactc aagcagattc aattgaagaa
    23461 atcgttgaag ttgtagaagg cgacaacgct taatttatac aaatagtaat tacctaggag
    23521 ggcggggctt agcccggctc tcctattttc aaaaaatata ggagaattaa aatggcagaa
    23581 attacagcta aacttgtaaa agagttgcgt gaaaaatctg gtgccggtgt tatggacgct
    23641 aaaaaagcgc ttgtagaaac agacggtgac atcgaaaaag cgattgaatt gcttcgtgaa
    23701 aaaggtatgg ctaaggcagc taagaaagct gaccgtgttg ctgcagaagg tttgactggt
    23761 gtttatgtta acggtaatgt tgcagcagtt attgaagtaa acgctgaaac tgacttcgtt
    23821 gcaaaaaacg ctcaattcgt tgaattggta aatactacag ctaaagtcat tgctgaagga
    23881 aaacctgcta acaacgaaga agctcttgct ttgataatgc cttcaggtga aactcttgaa
    23941 gctgcatacg tatctgcaac agcaactatc ggagagaaaa tctcattccg tcgctttgca
    24001 ttgattgaaa aaacagacgc acaacacttt ggagcatacc aacataacgg tggacgtatc
    24061 ggtgttattt cagttgttga aggtggagac gaagcacttg ctaaacaatt gtcaatgcac
    24121 atcgcagcga tgaaaccaac agttctttct tacaaagaat tggatgagca attcgttaaa
    24181 gatgagttgg cacaattgaa tcacgttatc gaccaagaca acgaaagccg tgcaatggtt
    24241 aataaaccag ctcttccaca cttgaagtat ggatcaaaag ctcaattaac tgatgatgtt
    24301 attgctcaag ctgaagctga catcaaagct gaattggctg cagaaggcaa accagaaaaa
    24361 atctgggaca aaattattcc aggtaaaatg gatcgcttca tgcttgataa cactaaagtt
    24421 gaccaagctt acacacttct tgcacaagtt tacatcatgg atgacagcaa gacagttgaa
    24481 gcataccttg aatcagttaa cgcttcggta gttgagtttg ctcgctttga agttggtgaa
    24541 ggtatcgaga aagctacaaa cgacttcgaa gctgaagttg cagctacaat ggcagcagcc
    24601 ttgaataact aataatagaa aggaagcaat ttgcttcctt ttttgtatag ggcttagatt
    24661 tttcctgaaa ttgttttttc cccagtctca ctcgattaga ataattaaaa caaaaagccc
    24721 tataataagt gctaagtgca tttaggagag tatacttcaa attcat
//
LOCUS       DAVDWT010000031        24217 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVDWT010000031 DAVDWT010000000
VERSION     DAVDWT010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24217
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.031"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..180
                     /locus_tag="VUW50_001547"
     CDS             <1..180
                     /locus_tag="VUW50_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7983420.1"
                     /translation="KLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSC
                     CNTFYEALLSCSCFN"
     gene            complement(387..2645)
                     /locus_tag="VUW50_001548"
     CDS             complement(387..2645)
                     /locus_tag="VUW50_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET7983421.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKNAIIIMTSNAGTGKTEASVGFGATREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2874..3104)
                     /locus_tag="VUW50_001549"
     CDS             complement(2874..3104)
                     /locus_tag="VUW50_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HET7983422.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3294..3977
                     /locus_tag="VUW50_001550"
     CDS             3294..3977
                     /locus_tag="VUW50_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET7983423.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3977..4711
                     /locus_tag="VUW50_001551"
     CDS             3977..4711
                     /locus_tag="VUW50_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET7983424.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESRKVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4865..5722
                     /locus_tag="VUW50_001552"
     CDS             4865..5722
                     /locus_tag="VUW50_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HET7983425.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5864..6736
                     /locus_tag="VUW50_001553"
     CDS             5864..6736
                     /locus_tag="VUW50_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET7983426.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            7018..7701
                     /gene="rpiA"
                     /locus_tag="VUW50_001554"
     CDS             7018..7701
                     /gene="rpiA"
                     /locus_tag="VUW50_001554"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET7983427.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7715..8926
                     /locus_tag="VUW50_001555"
     CDS             7715..8926
                     /locus_tag="VUW50_001555"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HET7983428.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAEINPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8928..9485
                     /locus_tag="VUW50_001556"
                     /pseudo
     CDS             8928..9485
                     /locus_tag="VUW50_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199123.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
     gene            9485..10294
                     /locus_tag="VUW50_001557"
     CDS             9485..10294
                     /locus_tag="VUW50_001557"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET7983429.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10770..11165
                     /locus_tag="VUW50_001558"
     CDS             10770..11165
                     /locus_tag="VUW50_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HET7983430.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEIIEPAQADDIRKVVGELAKDISITKLYMTGHSLGCYLAQIAAVEAYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            11175..11750
                     /locus_tag="VUW50_001559"
     CDS             11175..11750
                     /locus_tag="VUW50_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET7983431.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            11775..12287
                     /locus_tag="VUW50_001560"
     CDS             11775..12287
                     /locus_tag="VUW50_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HET7983432.1"
                     /translation="MSAQITINHKKARYVRIGLEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVTVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            12945..13655
                     /gene="deoD"
                     /locus_tag="VUW50_001561"
     CDS             12945..13655
                     /gene="deoD"
                     /locus_tag="VUW50_001561"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET7983433.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(13678..14202)
                     /locus_tag="VUW50_001562"
     CDS             complement(13678..14202)
                     /locus_tag="VUW50_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HET7983434.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     HEWMLTDIKNFLTKHKAWVIDGNYSWCCYQERMQEADQIIFLNFSPLTCLFRTFKRYL
                     KYRGKVRESMAADCPERFDWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(14255..14491)
                     /locus_tag="VUW50_001563"
     CDS             complement(14255..14491)
                     /locus_tag="VUW50_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET7983435.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(14559..15479)
                     /locus_tag="VUW50_001564"
     CDS             complement(14559..15479)
                     /locus_tag="VUW50_001564"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HET7983436.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            15669..16073
                     /locus_tag="VUW50_001565"
     CDS             15669..16073
                     /locus_tag="VUW50_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983437.1"
                     /translation="MKILKRYILELCFILSFALPFIKGANADNGRCFVETYYGFTFLM
                     EHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            16289..16870
                     /locus_tag="VUW50_001566"
     CDS             16289..16870
                     /locus_tag="VUW50_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7983438.1"
                     /translation="MYYAENPDAAHDIYELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            16867..18144
                     /locus_tag="VUW50_001567"
     CDS             16867..18144
                     /locus_tag="VUW50_001567"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HET7983439.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            18162..18824
                     /gene="deoC"
                     /locus_tag="VUW50_001568"
     CDS             18162..18824
                     /gene="deoC"
                     /locus_tag="VUW50_001568"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET7983440.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYGFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            18811..19200
                     /locus_tag="VUW50_001569"
     CDS             18811..19200
                     /locus_tag="VUW50_001569"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET7983441.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGNVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            19290..20342
                     /locus_tag="VUW50_001570"
     CDS             19290..20342
                     /locus_tag="VUW50_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HET7983442.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            20486..22021
                     /locus_tag="VUW50_001571"
     CDS             20486..22021
                     /locus_tag="VUW50_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7983443.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIYALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            22014..23072
                     /locus_tag="VUW50_001572"
     CDS             22014..23072
                     /locus_tag="VUW50_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7983444.1"
                     /translation="MFKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            23075..24031
                     /locus_tag="VUW50_001573"
     CDS             23075..24031
                     /locus_tag="VUW50_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7983445.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
     gene            24116..>24217
                     /locus_tag="VUW50_001574"
     CDS             24116..>24217
                     /locus_tag="VUW50_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7983446.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEKLSNKFGINNSN"
BASE COUNT         7058 a         4363 c         5488 g         7308 t
ORIGIN      
        1 aagctagaac ttttatgcga agagtttggg cataaacttt tacctcttcc tccctactca
       61 cctgagtaca atcctattga gaaaacatgg gctcatatca aaaagcacct caaaaaggta
      121 ttaccaagtt gttgcaatac cttttacgag gctcttttgt cctgctcttg tttcaattga
      181 ctatagcaga ttgaaataag atgagaacaa atcgattagg aaagtaaaat taatttctat
      241 aaatgtttta gcaattgttt cgtactattt cagattcagt ctactatata tcataatgga
      301 caaagggcaa gctgtcaaga aattacagtt attttagtaa aaaaagaagc agaaaaattt
      361 cattttctac tccttttcta taggatttat ttttcttttt cagaactttt aacttcagct
      421 tttttggcag atttaatctg aatgtttccc ttgctagtca taactgcttt gagatctttt
      481 tcgcttggat tttcaaggta gtagtcagtg attgtgtcct caatatagtc ttgaatagtc
      541 cgacgaagtg ggcgtgctcc catttttgga tcataaccta ggtcaaccaa cttttccttg
      601 accttgtcag ttacatccaa acgaatgttg ttgctagaga ggcgcttgtt aacatctgct
      661 agcatgagct cgacaatctg aaggaggtta tccttgctga gagccttaaa ttcgataatg
      721 ccatcaaaac ggttcataaa ctctgggcta aagaagttac cgagttcacc gaggacagaa
      781 ttggtacgtc cttctctagt agcaccaaat ccaacgctag cttcggtctt tcctgtacct
      841 gcatttgagg tcatgataat gatggcattc ttgaagctaa cggtgcgtcc ttgcccgtct
      901 gtcaaacgac catcgtccaa gacttgaaga aacatgtgca taacatctgg gtgagctttt
      961 tccacttcat cgagaaggat gagagaatat ggattgtggc gaactttttc agttaattga
     1021 ccagcctcat catagccaac ataacctgga ggagcgccga ccaacttagc tacactatgt
     1081 ttttccatgt attcactcat atcaaagcga atcatactat cagcagaacc aaaaagttcg
     1141 atagccagtt gtttggaaag ttctgtctta ccgacaccag ttggcccaac gaagaggaag
     1201 cttccgattg ggcggttagg ggtaccaagt ccgacacgat tacggcgaat agccttggca
     1261 atcttatcga ctgcatcatc ttgaccaata acatgagact tgagatcttc ggctagatgg
     1321 atgagttgag attgttcttt ctctttcaaa tcaccaacag ggatattggt tttctgctcg
     1381 ataatgtgct caatagtttt ctcgctgatg ataggagtat cctggtctgt gatctttttc
     1441 ttttgcattt ccttatactt ggcaatctgg tcgcggaagt aggccgcctt ctcaaaatct
     1501 tcttctcgtg tagcttgaga cttgagattt tcagcctcaa tcaagcgctg atcaattact
     1561 ttaggatcca caaaattcaa ggtcaagttc atcttagaac cagcttcatc taggaggtca
     1621 atggccttgt caggcaagaa gcgatcttgg atgtagcgat tggaaagagt tgcagctgct
     1681 tcaatcgcag catcggtata ttgaacgtgg tggtaatctt cgtatttctt ttgaatccct
     1741 ttgagaatag tgattgtttc gtccaccgtt ggttcatcga ctttaacagg ctgcatacga
     1801 cgctcgaggg cagcatcctt ttcaatgata cggtattcat tgagggtagt agcaccgact
     1861 agttgcagtt ctccacgagc aagggctggc ttgaggatat ttcctgcgtc catattacca
     1921 tcactcgcag aaccagcacc aacaatttca tggatttcat cgataaagag gatgatgtct
     1981 tcacgtttgc gaatttcttc catgagtttt tgcatgcgtt cttcaaattg tcctcgaatc
     2041 cccgttcctt gaactaagct aaccacatcc agacggatga cttgtttacc ttggagttta
     2101 tgtggcacat cgccatcgac aattttctga gctagacctt cgacaacggc cgtttttccg
     2161 acaccaggtt caccgataag gacaggatta ttcttggttc tacgattgag aatctcgatg
     2221 acacggataa tctcatcgtc gcgcccaata acggggtcaa tgtctccacg acgggcaatt
     2281 tcagttacat taataccaaa ttcttccagc aggccttttt cttggctagg tggcggagtt
     2341 tgagcagatc cacgattttg ggaaccataa ccgccgtttc caccgtaacc tccacctgat
     2401 tgggttgggg gaataggagg agtattgcta gaaggtctga aattgtttag atcattgaag
     2461 aaatcaccaa agggatcgaa gtcacgattg ttcagatccg tcataccttt gaagaggcta
     2521 ttgttaggat ctgttttgat aatcttatag cagttttgac agaggtcaat ttgtttttgt
     2581 tttccattga gattggtgta aagatgaatt gttgagtcgt taattttaca gttttgacaa
     2641 agcatacatc tacctcattt ctttctttag cctgacttgt ttaaaggtca aaaatagtca
     2701 aattctatac tctcattata acaggattgg ggtgtaaagc aagtaaaaag attgaagctt
     2761 tgagaagttg ttacaatgaa aatttttgtg aatttggact ataaaaaaat cctatttgtg
     2821 agacaaaaag gattttactt agacatgcag ggacaatccc agcttgtttt agattagtag
     2881 agaagttcgt aaatttccat tgcgattaag tcaatgctat caaacgtata cgtttcgcga
     2941 tcttctacgt cacgaagggt aaagattggg ccgttttctt cgtcgtggct gtacgcaact
     3001 tctgcaacaa caactccttc gcgttcaaaa ttacgtttta aatttccgcc atcgtttgcc
     3061 attgcttcaa ggcggttgat gattctaacc aaatgtgatt ccattttgat tctccttcat
     3121 ttcttatcgt tactatttta ccataaagga ggccaacttg actagaaaaa actaagattt
     3181 ctcgttaatt ctgctagttt aaagattttt aaataaattg gataaaaaat ttaaatttta
     3241 attcaaaaca tgttataatc atacagtatt ctattttaga aagcagtgtg actatgaatt
     3301 tttctttttt acctaagtat ttaccttatt ttaactatgg ggctgttgtg acgattctta
     3361 tttctatctg tgttatcttt ttgggaacta ttttgggtgt tgtcttggct tttgggcaac
     3421 gttcaaagtt taaaccgctt gtttggttgg ccaacttgta cgtttggatt ttccgtggga
     3481 caccgatgat ggttcaaatt atgattgcct ttgctcttat gcatatcaat gctccgacta
     3541 ttcagattgg aattttaggt gttgattttt cgcgtctgat tccagggatt ttgattatct
     3601 ctatgaatag tggtgcttat gtttcggaga ctgttcgtgc cggaatcaat gcggttccaa
     3661 aaggtcagct agaggcggct tattcgctag ggattcgtcc taaaaatgcg atgcgttatg
     3721 tgattttgcc acaagcagtc aaaaatatct tgccagcatt ggggaacgaa tttatcacca
     3781 ttatcaagga cagctccctc ttatcagcta ttggggtcat ggagttgtgg aatggggcta
     3841 caacagtttc tacaacaacc tatctacctt taacaccact tttatttgca gcattttact
     3901 acttgattat gacctctatt ctgacagtag ccttgaaagc ttttgaaaaa catatgggac
     3961 aaggagataa gaaataatga cagaaacctt gataaaaatt gaaaatttac ataaatcttt
     4021 tggaaagaat gaagtattga agggcatcaa cctcgagatt aaaagaggag aagttgtcgt
     4081 tatcatcggt ccttcaggga gtgggaaatc taccttgctt cgctctatga atttgttgga
     4141 ggaagcaacc aaggggaagg ttatctttga gggagtcgat attacggaca agaagaatga
     4201 cctgtttgcc atgcgtgaga agatgggcat ggttttccaa caatttaacc tctttcctaa
     4261 tatgactgtg atggaaaata ttaccttgtc acctatcaag actaaaggtg aaagcaggaa
     4321 agttgctgag aagagagccc aagagctttt ggaaaaagtt ggtttgccag ataaggcaga
     4381 cgcttatcca cagagtctgt cgggtgggca acaacaacgg attgccatcg cgcgtgggtt
     4441 ggctatggaa ccagatgttt tgctctttga cgaaccaact tcagccttgg atcctgaaat
     4501 ggtaggtgag gtattggctg ttatgcaaga ccttgccaag tcagggatga ctatggttat
     4561 cgtaacacat gagatgggat ttgcccgtga ggtggcagat cgtgttatct ttatggcaga
     4621 cggtgtggtt gttgaagatg ggacacctga gcagattttt gaacaaaccc aagaacaacg
     4681 gactaaagat ttcttgagta aggttttata atctatttta aaatttcaag acaagattag
     4741 tgaaaagttc aaccagagct ttttcttata gtttgaaact ataggataac ctaggaaaga
     4801 agtgttagag ggcactagca gtttttggta taatggaaga tatttgaaag aaaagagaag
     4861 tgatatgaca cagattattg atgggaaagc tttagcggcc aaattgcagg ggcagttggc
     4921 tgaaaagact gcaaaattaa aggaagaaac agctctagtg cctggtttgg tagtgatttt
     4981 ggttggggat aatccagcca gccaagtcta cgttcgcaac aaggagaggt cagctcttgc
     5041 ggctggttcc cgtagcgaag tagtgcgagt tccagagacc attactcaag aggaattgtt
     5101 agacctgatt gctaaatata atcaggatcc agcttggcat gggattttgg tccagttgcc
     5161 attaccaaaa catatcgatg aagaggcggt tttattagcc attgacccag aaaaggatgt
     5221 ggatggtttc catcccttaa acatgggacg tctttggtct ggacatccag tcatgattcc
     5281 ctcgacacct gcaggaatta tggaaatgtt tcatgaatat gggattgact tggaaggtaa
     5341 aaatgcggtc gtcatcggtc gttcaaatat cgttggaaaa cctatggctc agcttctttt
     5401 ggctaaaaat gcgacagtaa ccttgaccca ctcacgaact cataatcttg ccaaggtggc
     5461 tgctaaagca gatattcttg tagtcgcaat cggccgcgcc aagtttgtga ctgctgactt
     5521 tgtcaaacca ggtgcggttg tcattgacgt tgggatgaac cgagatgaaa atgggaagct
     5581 ctgtggagat gttgattatg aggcagttgc accacttgct agctatatca cgccagtccc
     5641 tggaggtgtc ggtcctatga ccattaccat gctgatggag caaacttatc aagcagcact
     5701 tcggacattg gatagaaaat aagagaaaca ttcgattaat agagtgtatt ttctatagct
     5761 atatctaaaa tggcagaaat gaatattaaa ttttagatat aagattacaa aaggaggtct
     5821 gcgtctcctt tttattgtat aataaaagtg agaggaaaaa aggatgaaag tgattaatca
     5881 aaccttatta gaaaaagtta ttattgaacg ttctcgttca agtcataaag gagattatgg
     5941 tcgtctgctg ctgcttggtg gtacttatcc ttatggaggt gccatcatca tggctgcttt
     6001 agcagctgta aaaagcggtg caggattggt gaccgttgga acggacaggg aaaatatccc
     6061 ggctctgcac agccatttac ctgagcctat ggccttttct cttcaagacc agcaattgtt
     6121 aaaagagcaa ttggagaagg cagaagttgt cttgttgggg cctggtttac gagacgatgc
     6181 ttctggagaa aatctagtaa aacaggtctt tgttaattta agccaaaatc agattttgat
     6241 tgtagacgga ggtgccttga ccattcttgc taggacaagt ttgtcatttc cctcaagcca
     6301 gcttatccta actcctcacc aaaaagaatg ggaaaaatta tctggaatta cgattgaaaa
     6361 gcaaaaggaa gatgcaacgg ctagtgttct gacttccttc cctcaaggaa caattttggt
     6421 ggagaaaggc ccagctactc gtatttggga agttggccaa tctgattatt accagttaca
     6481 ggttggtggt ccttatcagg cgactggggg aatgggagat actctggctg ggatgatcgc
     6541 aggatttgta ggccaattcc gacaggccag tctctacgag cgtgtggcag tagcgaccca
     6601 tcttcattca gccatagccc aagaactatc tcaggaaaat tatgtggtct taccgacgga
     6661 aatcagcagg tatcttccca aaataatgaa aattatttgt caacaagaga gggtaagcaa
     6721 agataaactt gtttaaaata gacagataaa tctacttcct tctcttaatt tatgattctt
     6781 gaaatgcgcc tgctttaagg ctaaactaga cgtatcaaag ttaagagttt gtttttgtaa
     6841 ctggaaagag atgaacaaat caaatacata aaagataaac tcatttttat tcgtttggta
     6901 aaaataaaat gcatattttt aaagaaagat tgatgaaaaa tgtagatttg atgtttttac
     6961 ttgaataaaa agcggtttca tagtaaaata agaactgaga atgatggagg taaatcggtg
     7021 gaaaatctga agaaaatggc aggtatcaag gctgctgagt tcgtgagcga tggaatggtc
     7081 gttggacttg gaacaggctc gactgcctat tattttgtcg aagaaatcgg tcgtcgtatc
     7141 aaggaagaag gattgcagat tacagctgta acaacttcta gtgtgaccac taaacaggca
     7201 gaaggcctta atatcccgct caagtctatt gaccaagtag actttgtcga tgtgacggtt
     7261 gatggcgcgg atgaagtgga tagtcagttt aatggaatca aaggcggtgg tggtgccctt
     7321 ctcatggaaa aggtggtcgc aacaccatca aaagaataca tttgggtggt ggatgaaagc
     7381 aagctggtcg aaaaactagg tgcttttaaa ttgccagtag aagtggttca gtatggtgca
     7441 gagcaggtct ttcgtcattt tgaacgagct ggctacaaac caagtttccg tgaaaaagac
     7501 ggccaacgtt ttgtgacgga tatgcagaat tttatcattg acctcgcctt ggatgtcatt
     7561 gaaaatccaa ttgcttttgg acaagaattg gaccatgtcg ttggtgttgt ggagcatggt
     7621 ttattcaacc aaatggtgga taaggtaatc gttgctggac gagatggagt tcagatttca
     7681 acttcaaaaa aaggaaaata gaagggggca taagatgtct aaatttaatc gtattcattt
     7741 ggtggtactg gattctgtag gaatcggtgc agcaccagat gctaataact ttgtcaatgc
     7801 aggggttcca gatggagctt ctgacacact gggacacatt tcaaaaacag ttggtttgaa
     7861 tgtcccaaac atggctaaaa taggtcttgg aaatattcct cgtgaaactc ctcttaagac
     7921 tgtagcagct gaaatcaatc caactggata tgcaacaaaa ttagaggaag tatctcttgg
     7981 taaggatact atgactggac actgggaaat catgggactc aacattactg agcctttcga
     8041 tactttctgg aacggattcc cagaagaaat cctgacaaaa atcgaagaat tctcaggacg
     8101 caaggttatt cgtgaagcca acaaacctta ttcaggaacg gctgttatcg atgattttgg
     8161 accacgtcag atggaaactg gagagttgat tatctatact tcagctgacc ctgttttgca
     8221 gattgctgcc cacgaagaca ttattccttt ggatgaattg taccgtatct gtgaatacgc
     8281 tcgttcgatt acccttgagc gtcctgccct tcttggtcgc atcattgctc gcccttatgt
     8341 aggtgaacca ggtaacttca ctcgtacggc aaaccgtcgt gacttggctg tatctccatt
     8401 ttccccaact gttttggata aattgaatga ggctggtatc gatacttatg ctgtgggtaa
     8461 aatcaacgat atctttaacg gtgctggtat caaccatgac atgggtcaca acaagtcaaa
     8521 tagtcatgga attgatacac tattgaagac tatgggactt gctgagtttg aaaaaggatt
     8581 ctcattcaca aacctagttg actttgatgc cctttacggc catcgtcgta atgctcacgg
     8641 ttaccgtgat tgtttgcatg agtttgatga acgcttacct gaaattatcg cagctatgag
     8701 agagaatgac cttctcttga ttactgcgga ccatggaaat gacccaacgt atgcagggac
     8761 ggatcacact cgggaatata ttccattgtt ggcatatagc cctgccttta aaggaaatgg
     8821 tctcattcca gtaggacatt ttgcagatat ttcagcgact gttgccgata actttggtgt
     8881 ggaaactgct atgattgggg aaagtttctt agataaattg gtataagatg acgcgctatg
     8941 ctttgctggt gagaggtatc aatgttggta gtaagaataa ggtcgttatg gcggagcttc
     9001 gtcaagaatt gacaaacttg ggactggaaa aggttgagag ctacatcaac agtggcaata
     9061 ttttctttac ttcgatagat tccaaagccc aattggttta aaagctagag actttctttg
     9121 cagtccatta tccatttatt cagagctttt ctttactgag tctagaggac tttgaggcgg
     9181 aacttgaaaa tctaccagct tggtggagca gagacttggc acgaaaagat tttctctttt
     9241 acactgaggg tttggatgtg gaccaagtca tcgcgacagt tgaaagttta gagctgaaag
     9301 atgaagtgct ttattttaga aaacttggga ttttctgggg gaaattttct gaagaatcct
     9361 attctaagac tgcctatcat aagtacttgc tgaaggtgcc tttctaccgc cacattacta
     9421 ttcgtaatgc taaaaccttt gacaaaattg gtcaaatgct aaaaaataat aaaggagaca
     9481 cataatgaca tttttaaaca aaatccatga aactgctact ttcctgaaag aaaagggaat
     9541 tgcagcccct gagttcggtc taatccttgg gtcaggactt ggagaattgg cagaagaaat
     9601 cgaaaatcca gttgtagtag actatgctga gattccaaac tggggccgtt caacagtagt
     9661 cggtcatgct ggtaaattgg tatatggtga actggcaggt cgcaaggtct tggctcttca
     9721 agggcgtttc catttctatg aagggaatcc tctggaagtg gtgactttcc cagttcgtgt
     9781 gatgaaagtt cttggatgtg aaggtgttat tgtaaccaat gcagctggcg gtatcggatt
     9841 tggtcctggt accttgatgg ctatctcaga ccatatcaac atgacggggc aaaatccatt
     9901 gatgggtgaa aacttggatg actttggccc acgtttccca gatatgtcta gggcctacac
     9961 accagaatac cgtgccactg cccatgaagt ggctaaaaaa cttaatatca agcttgatga
    10021 aggtgtctat atcggagtta ctggtccgac ttatgaaaca ccagcagaaa ttcgttccta
    10081 taagacactg ggagcagatg cagttggtat gtctacggtt cctgaagtta tcgtggcggc
    10141 ccactctggc ttgaaagttc tgggaatttc atgtatcact aactttgcgg ccggtttcca
    10201 agaagaactc aatcacgaag aagttgtaga agtgactgaa cgtgttaaag gtgatttcaa
    10261 aggcttgctt aaagcgattc ttgctgaatt gtaagaaaaa agatttaaaa gggggagtgc
    10321 ctctgttttt tcaggattga ctgcctatcc ggattaaaga agaaacagag gaatactatg
    10381 agcttcttcc tgctcttata actgaaagaa gcggaagaat aggtgtgtct gatctgatag
    10441 ccagcattgt gaaagacaag attctaggat actagcatta gcttcctagc caagtagact
    10501 agtatgataa ggagagatga gaatgaattg actttctgaa tttctcagtc ttatcatata
    10561 tagcacaatg agatttcgct tgagtctgct tgtaaataaa cgaaaagaaa gataagaaat
    10621 aatgaaaatt ggtcaacgaa ttatgcgctt tggcataaaa aattaagtat cggagttgta
    10681 tctgttgtag tcggctttga tttctagctc cagctggaat ttcagccaat gaagtaaagc
    10741 aagatgtaac atctgaagtg gtaataggtg tgctagattc taaggaggaa ttgaaagagt
    10801 cagaaaatga tgctccaaaa ctagaaactc ctcttagaga ggagccaaga ctagctcctc
    10861 aaacgcttcc ggaagcaagt gaagttcttg aaaacaaaag ggaagagtca aaagtagaga
    10921 taatagagcc agctcaagcg gatgatatcc gcaaggttgt tggggaatta gccaaggata
    10981 taagtattac taagttgtat atgacaggtc attctcttgg atgttaccta gctcagattg
    11041 cagcggttga agcttaccaa aaatatcctg atttttataa ccatgtattg aggaaagtga
    11101 caactttcag tgctcctaaa gtgattactt ccagaactgt ttgggatgct aagaatggtt
    11161 tctgagatgt tggtttggaa agtcgtaaat tagctgttag tggaaaaatt aagcattatg
    11221 tggttgataa tgacaatgtt gtgactccct tgattcataa taatcgtgat attgttacat
    11281 ttacaggtaa ttcacgcttt aaacaccgtt ctcgtggcta ttttgaaagt ccaatgaatg
    11341 atattcctaa ctttaatatt ggtaaacaag ctaccttgga taaacatggt tatcgtgatc
    11401 cgaaattgga taaagtgcga ttctttaaga aacaggctct acctcaatct tctagtcaac
    11461 caagcgctga accaatggaa aatattgcct taggaaaaca ggttactcaa agttcgacag
    11521 ctttcggagg agatgctaga agagctgtgg atggcaaagt cgatggtaac tatggtcaca
    11581 attctgtcac tcatacaaac ttccaatcta agccttggtg gcaagtagat ttggctaaag
    11641 aagaaaccat tcgccaaatc aatatttaca accgaacaga cactgcccag gatagattgg
    11701 caaactttga tgtcattctt ttagacagtt ctggtaaaga aattgagtga aaacgtataa
    11761 tatctcctaa agatgtgtca gcacaaatta cgattaacca taaaaaagcg cgctatgttc
    11821 ggattgggct agaaggctat aatgccctca gtcttgcaga agttgaagtt ttccgcttta
    11881 tagctacgaa tgctgaaacg gcgacacaag tttctaagcc ggttcaacca atcagtcaga
    11941 ctcctgtgaa agataaaaca ttgacaattc aacacagtgg agcttacatt gcccgctact
    12001 ccataacttg ggaagaagtt acagtagata aagatggaaa ccaagttgtt cgtagtcatt
    12061 cttgggaagg aaacggtcgc aaccagactg caggttttgt cctcaacctc ccaatcaaag
    12121 aaaatatgag aaatctgcga gttaagattg agaaaaagac gggcctacta tggaatagat
    12181 ggcaaacaat ctatgaaaac agaccaattt tagctcaacc ccaccgtaaa attacccatt
    12241 ggggtacgac attgaattcc aaggtgagtg acgatgatgt cttgtaatct gatggtacaa
    12301 tgacagttag tttgtctagt ttataagaaa gtactacctg agcttgaata gaactcaggt
    12361 agctctctgt gaaagaacaa aattaatact caatgaaaat caaagagcaa actaagaaac
    12421 tagccgcagg ttgctcaaag cactgctttg aggttgtaga taagactgac gaagtcagtc
    12481 acatatataa tccaaggcga cgttgacgtg gtttgaagag attttcgaag agtataaaca
    12541 gaaaggtaga gcgcgtgttc taatttgaac acgagtagaa aacttttcta aaaataaaaa
    12601 cgaaaggatg ggtaaactgt attcgctgaa ctgaatacgg gcgactctcc tctaaatcaa
    12661 aattaagaaa gaaaggaatt gaccccaccc taaaagcagt gggaaaaaga tagttggtct
    12721 agcgagcatc gctcactgcg cccaactcct attttccctt cgctttttga tgggtttggt
    12781 atctttctca atataaaata taaaataaag aaaggtagag cgtgtgtttt gatttgaaca
    12841 cgagcggaaa actcggaaaa tagataatct gactgaaaaa tcaggatttc tcgtcaggtt
    12901 cctaattttc agtcgttttc ttctcgctct ttgtatcata aattatgtct atccatattg
    12961 ctgctcagca gggtgaaatt gctgataaaa ttcttcttcc tggggatcct cttcgtgcta
    13021 agtttattgc ggagaatttc cttgatgatg ctgtttgttt taacgaagtg cgtaacatgt
    13081 ttggttacac tggtacttac aagggtcact gtgtatctgt catgggaact gggatgggaa
    13141 tgccatctat ttcgatttat gcgcgtgagt taatcgtaga ctacggtgtg aagaaattga
    13201 ttcgtgtggg aactgcaggt tctttgaatg aagaggttca tgttcgtgaa ttagttttgg
    13261 cgcaggcggc tgcaaccaac tcaaacatcg ttcgtaatga ctggccacag tacgattttc
    13321 cacaaattgc tagctttgat ttgcttgata aagcctacca tatcgccaaa aaacttggta
    13381 tgactactca cgttgggaac gttttgtcat ctgatgtctt ttactcaaat tactttgaaa
    13441 agaatatcga gcttggtaaa tggggagtca aggctgtgga aatggaagca gcagctcttt
    13501 actatcttgc tgcccaatac catgttgatg cgctagctat catgaccatc tctgatagct
    13561 tggtcaatcc agacgaagac acaactgcag aagaacgtca aaataccttc actgatatga
    13621 tgaaggttgg tttggaaacc ttgattgcag aataattata gccaaaaagg aagcccttta
    13681 tgaattgtag gacttccttt tcttatccag aaattgatct agctctctct gatttcgaag
    13741 aatagtgact ttatgtgaat attcttggca aagtttttgg taattttctt tttgagtttt
    13801 gctacgccca tcccaaagaa tccatctgat aaactcccag tcaaagcgtt cagggcaatc
    13861 tgccgccata ctttctctga cttttccacg gtatttaaga taacgcttaa aggttctaaa
    13921 gagacaggtc aatggcgaaa aattgagaaa gatgatttgg tcagcttctt gcattcgttc
    13981 ttggtagcag caccaagaat aattaccatc gatgacccaa gctttatgct tggtgagaaa
    14041 gttttttata tcggttaaca tccattcgtg gtcactgtct tgccaaccag gttgaaattg
    14101 gagtgtgtcc atgtgcagtt ttggaatgga gtagtagtta gataactttt ctgctagagt
    14161 tgacttacca gaaccagaat atccgataat tgcgattttc attttctacc ttttcctatt
    14221 tggagacaaa aaaacagcct ctatggactg tttcttattt agcaagttta gctgaaagac
    14281 gagctttatc gcggcttgct ttgtttttgt gaatcaaacc tttagtttct gctttatcga
    14341 tagctgagct agcagcacgg aaaagttctt cagatgggtt tgcttcgaaa gcttttatag
    14401 cagtacgcat agctgatttt tgagctgagt tcttttcgtt ttgtttaacg ttcaattcag
    14461 cgcgtttgat agctgattta atgtttgcca atattcttac ctccatattt actaactata
    14521 ccattatata tgaaaactta cgttttgaca agggaaaatt acttttttaa gtaaatttca
    14581 tcgatttcat ggttcttgct tttatgaaga atcacttctg cacgatttct ggttggttca
    14641 atataatttt gcagatttgt gagattgata ctgatccaga cctgatgggc aaaggactcc
    14701 acttccccaa tcggcatctg agtaaaacga taatagtagc tatcagggtc gttttgggct
    14761 aggctcagca ttttcaagaa acggtccaga taccaacttt caatatcatc cactccagca
    14821 tctacataga tggaaaagtc aaagaagtca gtgatataga gacgatcgtt ttgtggattt
    14881 tgaaagacat tgattccctc aacaattaca aaatcagcag ctttgacact ttgtttctct
    14941 tcgggtacga tgtcgtaaac ttcatgagaa tagacaggaa tatctacatc ttgtccattt
    15001 ttgatgcggt ccaagaagtt gagaagagct tccatatcat agctttcagg aaatccttta
    15061 cgatttaaaa tcccctgctc aatcaaggtt tgattgggat agagaaaacc atcagttgta
    15121 accaactcaa ccgtagcatc tgtaaacgta cgggacagta ggatttgaag taggcgactg
    15181 gttgtggatt ttccaacggc aacactccca gaaaccccaa taataaaagg ttgagattta
    15241 ctttcacgtt ggaggaaaat tccttttgaa aaggctaaat cttccttagt tcgcttgtaa
    15301 atctgaatca gatgagccaa ggggagatag atatctgtaa cgtcttggag actgatttgg
    15361 tcattaaaac tcttgataga ttccaattct tcttctgtca aaggaggtgt tgtctttcga
    15421 tgtaaagatt gccaagtctg gcggctgatt ttttcaaaat gtaaaaattc gttggtcatt
    15481 tgttttcccc taggtatttt atctatcata ccataaaaag ctaaaaaaat aaagcggttt
    15541 cttgaatgtt gtaagtcaat gatatataat ataagcgatc aatttattga tgaaatagga
    15601 gatagattct actgatttat aagcttataa agggggcttt ttagttcaca acttatagga
    15661 ggtgtactat gaaaatctta aaacgttaca tattggaact ctgttttatt ttaagttttg
    15721 ctttaccttt tataaaagga gccaatgcag ataatggtag atgctttgtg gaaacctatt
    15781 acggttttac ttttttgatg gaacatgcta ttgtaacagc tgtctttatc tattcgtttt
    15841 taattgcttt cttactaaaa aaacgatggg cgaaatggat tgctgctggt agttattgct
    15901 ttttagtcct atggattgcg acagagggtt atttcttccg catgtcacta gaagatttga
    15961 tacgactttg gacaagtttg gaaatcctga cacaaacgta tcagttgggc ttttatctaa
    16021 acattttgtt gggaattttg ttgataataa agtattttaa ggttaagcaa tagaaataat
    16081 aatctgcctg attatagatg ataaactttt tgtttttatc tggactgtga tatttatagt
    16141 tctaaatggt ttattattta taagtgttga aagagattga agaaaatggc ttcgtgactc
    16201 gctttcttcg gtctttttcg ttttattatc aatttgtaaa cgatttacaa tttgagtcaa
    16261 attatggtaa aatagtgtca tgagtaaaat gtattatgca gaaaatcctg acgctgctca
    16321 cgacatttat gagttgagag tagagttgtt gggacaaaaa atgacctttt taacggatgc
    16381 gggtgttttt agcaagaaaa tagttgactt tggaagtcaa ctattgctca agtgtcttga
    16441 ggtcaaccaa ggagaaactg tccttgatgt aggctgtggt tacggacctt tgggtttgtc
    16501 cttagctaag gcttatggag ttcaggctac tatggttgat atcaacactc gtgccttaga
    16561 tttagctcgg agaaatgctg aaaaaaataa tgcaaaagcg acgattttcc agtccaacat
    16621 ctatgaacaa gttgaaggac attttgatca tgtcatttcc aatcctccta tccgtgcggg
    16681 caagcaagtt gttcatgaaa tcattgagaa aagtaaagat tttttggaaa ctggtgggga
    16741 tttgacaatc gtcatccaga aaaaacaagg ggctccaagt gctaagtcca agatggagga
    16801 tgtgtttggt aattgtgaaa tccttaaaaa agacaaagga tactatatcc ttagaagtgt
    16861 gaaagaatga gagcagttga tttaatccaa aaaaaacgag acggtcaaga actgacttca
    16921 agtgaaattg aatggctgat agaaggctat gtatcaggaa ctgttcctga ttatcagatg
    16981 tctgcctttg ctatggctgt ttattttaaa ggaatgacga ctcgagaaat ctctgattta
    17041 acgatgaata tggttaagac tgggcaagag tttgacctgt cagccattga tggtgtgaaa
    17101 gttgacaagc attctactgg tggtgtcggc gataaggtaa ccttgatttt agctcctctt
    17161 gttgctagct tcggtgtgcc tgtagcaaaa atgagtggtc gtggtcttgg ccatacaggc
    17221 ggaacaattg ataaattgga gtccattaag ggctatcaag tagaacgtag tcaagaggat
    17281 tttattcgtc aggttcagga tattggagta tctgtcattg ggcaatcaga ccagctggtc
    17341 aaagcagata agcttctcta tgcccttcgt gatgtgaccg caactgtcga cacgattcct
    17401 ttgattgcga gttcggtgat gagcaagaaa attgcggcag gggcggatgc tattttgcta
    17461 gacgtgactg tcggtgaggg tgccttcatg aagacggttg atgaggcgcg tgagttggct
    17521 caaactatgg tagatcttgg taaggttgtt ggacggaaga cagtagcagt gattacggat
    17581 atgagccagc ctttgggacg agcgattgga aatcgtctgg aaatccttga agcattggag
    17641 attttgcaag gtcaaggacg tcaggatatt acccacttta tttgtgaatt ggctcaaatt
    17701 atgcttggtt tggcaaatgt taacaaaact gttgaagaag ttcgccaaca tcttgagaat
    17761 ggtcaagcac tggctaagtt tgaggaaatg gtgcaagccc aaggtggaga cttggaagac
    17821 ctctatcgtc ctgtaaatgt agctcatgtg gtggaaatcc ctgctcagga aacgggtgtc
    17881 atttcagctc ttccagctat ggactttggt ctttatgcca tgaggctggg tgctggtcgt
    17941 gcagttaagt ctgatgcctt ggactatgaa acaggaattg tttttgaaaa gaagattgga
    18001 gactccgttc aaaagggtga aattgttgca aaagtataca caaatgaaaa aattccacct
    18061 caactagtta cagatttcca aaaatgtgtt aaaataagtg atgaagtgaa aaaaatccgt
    18121 gaaattgtag aaatcatctc ctaaatggag ggaaaaccga aatgaaatta aataaatata
    18181 tagatcatac gcttttaaaa caagatgcaa agaaaaaaca aattgatagt ttgttgtctg
    18241 aggctagaga gtatggcttt gccagtgttt gcgttaatcc aacctgggtt gaacatgcta
    18301 aaaaagggct tgaaggcaca gatgttaagg tttgcacggt agtaggtttc cctttgggag
    18361 caacaacttc agccgtgaaa gcatttgaga caaaagaagc tatccaaaat ggtgcagatg
    18421 agattgatat ggtgatcaat gttggagctc tcaaatcagg taatttagcc ttggttgagt
    18481 cagatattcg cgcagtagtg gaagcaagtg gtgataagtt agtgaaagtc attattgaag
    18541 cttgccttct gacagaccaa gaaaaagttg ttgtttgcca attggcccaa aaagctgggg
    18601 ctgactttgt caaaacatct actggctttt caactggtgg tgctacgata gcagatgtta
    18661 cattaatgcg tgaaacagtt ggatctgata tgggagtcaa ggctgctggt ggagctcgtt
    18721 cttatgcaga tgctcttgcc tttgtcgaag caggtgcgac ccgtatcgga acgtcagctg
    18781 gggtagctat tttaaaagga gaattggcag atggcgacta ctgagttgat tgaactggca
    18841 attgaaacca gcaaacatgc ctatgtcccc tattctcact ttcctatcgg agcagtttta
    18901 gtagccaaag acggaaatgt ttacacggga gtgaatatcg aaaatgctag ctatcctttg
    18961 accaattgcg gtgagagaac agctattttt aaggctatat ctgaaggcca aagagaattt
    19021 tcagaattga ttgtctatgg tcaaactgaa aaaccgattt caccatgtgg tgcttgtcgc
    19081 caagtaatgg tcgaattttt tgaacaagat ttaaaagtga ccttagtcgc aaaagataaa
    19141 tcgacggtcg agatgacggt cggggaatta cttccatact cttttacaga cttaaactag
    19201 tctgagtcgc tctctgagtg gcacggtcct tgtggccaac caatccatac ttgcaacatc
    19261 gttgcacatc ttatttagga ggttcagtaa tgaacaagaa acaatggcta ggtcttggcc
    19321 tagttgcagt ggcagcagtt ggacttgctg catgtggtaa ccgctcttct cgtaacgcag
    19381 cttcatcttc tgatgtgaag acaaaagcag caatcgtcac tgatactggt ggtgttgatg
    19441 acaaatcatt caaccaatca gcttgggaag gtttgcaggc ttggggtaaa gaacacaatc
    19501 tttcaaaaga taacggtttc acttacttcc aatcaacaag tgaagctgac tacgctaaca
    19561 acttgcaaca agcggctgga agttacaacc taatcttcgg tgttggtttt gcccttcata
    19621 atgcagttaa agatgcagca aaagaacaca ctgacttgaa ctatgtcttg attgatgatg
    19681 tgattaaaga ccaaaagaat gttgcgagcg taactttcgc tgataatgag tcaggttacc
    19741 ttgcaggtgt ggctgcagca aaaacaacta agacaaaaca agttggtttt gtaggtggta
    19801 tcgaatctga agttatctct cgttttgaag caggattcaa ggctggtgtt gcgtcagtag
    19861 acccatctat caaagtccaa gttgactacg ctggttcatt tggtgatgcg gctaaaggta
    19921 aaacaattgc agccgcacaa tacgcagccg gtgcagatat tgtttaccaa gtagctggtg
    19981 gtacaggtgc aggtgtcttt gcagaggcaa aatctctcaa cgaaagccgt cctgaaaatg
    20041 aaaaagtttg ggttatcggt gttgatcgtg accaagaagc agaaggtaaa tacacttcta
    20101 aagatggcaa agaatcaaac tttgttcttg tatctacttt gaaacaagtt ggtacaactg
    20161 taaaagatat ttctaacaag gcagaaaaag gagaattccc tggcggtcaa gtgatcgttt
    20221 actcattgaa ggataaaggg gttgacttgg cagtaacaaa cctttcagaa gaaggtaaaa
    20281 aagctgtcga agatgcaaaa gctaaaatcc ttgatggaag cgtaaaagtt cctgaaaaat
    20341 aatggatgga agtcatttct taggaagcgg ccaagggccg cttttttaag agttgagaca
    20401 aagtcatttt gtctcagtaa agcttgctac tagacaaact agcaagtttt attgaaataa
    20461 aataagactc tgaaaggaag agcacatggc acacgaaaat gtcattgaga tgcgtgatat
    20521 taccaaggtg tttggtggat ttgttgccaa cgacaaaatc aacttgcacc tacgaaaagg
    20581 tgaaatttat gcacttttag gagaaaatgg ggctggtaag tccacgctaa tgaacatgtt
    20641 agcaggcctt cttgaaccaa ctagtggtga aatcgcggtc aacggtcaag ttgtcaatct
    20701 cgactcccca tctaaagcag ctagcttggg aatcgggatg gttcaccagc actttatgtt
    20761 ggttgaagcc ttcacagtgg ctgaaaacat cattttaggt agtgaattga ctaaaaatgg
    20821 tgtgctagat atcgctggag ctagcaaaga aatcaaggct ctttctgaac gttatggctt
    20881 agctgttgac ccttctgcca aggtagcaga tatctcagtt ggagcccaac aacgtgtaga
    20941 aattttaaaa acactttatc ggggggctga tatccttatc tttgacgaac caacggctgt
    21001 tttgactcca tcagaaattg atgagttgat ggctattatg aaaaatcttg tcaaagaagg
    21061 aaaatcaatt atcttgatta cccacaaatt ggatgaaatt cgcgcagttt ctgaccgtgt
    21121 tacagttatc cgtcgtggga aatcaattga aaccgttgaa attgcagggg ctaccaatgc
    21181 tgatttggcg gaaatgatgg taggacgttc tgtttccttt aaaacagaga agcaagcctc
    21241 taaaccaaaa gaagtggttt tgtctatcaa agatttggtg gtcaatgaaa accgtggtgt
    21301 tccagctgtt aaaaatctgt ccttggatgt tcgtgctgga gagattgttg gtattgcggg
    21361 gattgatgga aatggtcagt ctgaactgat tcaagccatt acaggtcttc gtaaggttga
    21421 atctggtagc attgagctaa aaggggattc aattgtaggc ttgcacccac gtcagattac
    21481 agaactaagt gttgggcacg ttccagaaga ccgtcaccgt gatggcttga ttttggaaat
    21541 gatgatatct gaaaatattg cccttcaaac ctactataaa gaaccacata gtaaaaatgg
    21601 aattttgaat tattcaaata ttacttctta tgctaaaaag ctgatggaag agtttgatgt
    21661 tcgcgctgcc agtgaattag ttcctgcagc tgcactctca ggaggaaatc aacaaaaagc
    21721 aattattgct cgtgaaattg atcgagatcc tgatctcctt atcgttagcc agccaactcg
    21781 tgggttggat gtcggtgcca ttgagtatat ccacaaacgc ttgattgaag agcgtgataa
    21841 tggcaaggct gtccttgttg tcagctttga attggatgag attttaaacg tctcagaccg
    21901 tattgccgtt atccacgatg gtaagattca aggtattgta tcaccagaaa caaccaataa
    21961 acaagaactt ggtgtcttga tggctggtgg aaacttggga aaggagaaga gtgatgttta
    22021 aaaaattaca acaaatttcg gttcccttga tttctgtatt tctaggaatt ttactcggag
    22081 ccattgtcat gtggatcttc ggttatgatg ctatttgggg ctacgaagaa ttgttctata
    22141 cagcctttgg cagtctgcgt gggattggag aaatcttccg tgctatgggt cctctggtct
    22201 tgattggtct tggttttgcg gttgccagtc gagctggttt ctttaacgtc ggacttcctg
    22261 gtcaggcttt ggcaggttgg attctcagtg gttggtttgc gctgtcgcat ccagatatgc
    22321 cccgtccctt gatgattcta gcaaccatcg tgattgcctt gattgctggt gggattgtcg
    22381 gagcgattcc aggtattctt agggcctatc tagggacgtc agaggttatt gtaaccatca
    22441 tgatgaacta cattgtcttg tatgtaggga atgcctttat ccatgctttc cctaaagact
    22501 tcatgcaaag tacagattcg accattcgtg ttggggctaa tgcaacctat cagacacctt
    22561 ggttggctga gttgactggt aactcacgga tgaatattgg tattttcttt gccatcattg
    22621 ccgttgcagt tatttggttc atgctcaaga aaacaactct tggttttgaa atccgtgcag
    22681 ttggtcttaa tccacatgct tcagaatatg ctggtatttc tgccaagcgg actattatcc
    22741 tatctatgat tatttcaggt gccttggcag gtcttggtgg agctgttgaa ggtttgggaa
    22801 ccttccagaa cgtctatgtt caaggttcgt cattagctat cggatttaac ggaatggcgg
    22861 ttagtttgct tgcggccaac tcaccaattg gtatactctt tgcagccttc ctatttggcg
    22921 ttctccaagt tggggctcct ggtatgaatg cggcgcaggt accatctgag cttgtcagca
    22981 ttgtaacagc gtctattatc ttctttgtca gtgttcatta ccttatcgaa cgctttgtta
    23041 aaccgaaaaa acaagttaaa ggaggtaagt aaagatgtct attataacct tgctccccct
    23101 cttggtgtct tctatgctga tttactcagc acctctcatc tttacaagta tcggtggtgt
    23161 tttctctgaa cgtggtggtg tggtaaacgt tggccttgaa ggaattatgg ttatgggtgc
    23221 cttttctgga gttgtcttta accttgaatt tgcagaacaa tttggagcag caactccatg
    23281 gctatccttg cttgtagcag gattggttgg tagtgttttc tctatcatcc acgcagcagc
    23341 gacggttcat ttccgtgcag accatgttgt cagcggtacg gtattgaact tgatggcgcc
    23401 tgccttggct gttttcttag ttaaagttct ttataacaaa ggacaaaccg acaacctaag
    23461 tcaaactttt ggacgctttg atttcccagt cttggcaaat atcccagtga ttggtgatat
    23521 cttcttcaag tcaactagtc tacttggtta tctggcgatt gccttctcat tccttgcttg
    23581 gtttattctc tttaagactc aatttggtct tcgtctccgc tctgtcggtg aacatcctca
    23641 agcagcggat accttgggaa tcaacgtcta caagatgaga tatttaggga ttattatttc
    23701 aggttttcta ggtggaattg gcggagcgat ttatgcgcaa tcaatctcag ttaacttctc
    23761 agtgacaact attgttggac ctggatttat cgcccttgct gcgatgatct ttgggaaatg
    23821 gaatccaatc ggtgctatgc tttctagtct cttctttgga ctttcacaaa gtttggctgt
    23881 tatcggttct caattgccgt tcctacaagg agtgccagcg gtttatcttc aaattgcacc
    23941 ttatgttttg acaattcttg tcttagcagc cttctttgga aaagcagtcg caccaaaagc
    24001 agatggtatc aactacatca aatcaaaata agcatacaaa aaaacgtcag ttcaatgact
    24061 gaactgcccc ccaaaagtta gacagaaaaa atctaacttt tggggtgttt ttattatgaa
    24121 actaacttat gatgataaag ttcagatcta tgaacttaga aaacaaggat atagcttaga
    24181 gaagctttca aataaatttg ggataaacaa ttctaat
//
LOCUS       DAVDWT010000032        22914 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVDWT010000032 DAVDWT010000000
VERSION     DAVDWT010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22914
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.032"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            334..1410
                     /locus_tag="VUW50_001575"
     CDS             334..1410
                     /locus_tag="VUW50_001575"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET7983447.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            1466..2401
                     /gene="dapA"
                     /locus_tag="VUW50_001576"
     CDS             1466..2401
                     /gene="dapA"
                     /locus_tag="VUW50_001576"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET7983448.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(2448..3821)
                     /gene="mnmE"
                     /locus_tag="VUW50_001577"
     CDS             complement(2448..3821)
                     /gene="mnmE"
                     /locus_tag="VUW50_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET7983449.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTNSFAIAQKIFKGKDL
                     SQVASHTLNYGHIVDPLTGKVMDEVMIGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(3959..4141)
                     /locus_tag="VUW50_001578"
     CDS             complement(3959..4141)
                     /locus_tag="VUW50_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HET7983450.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAIVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            4265..4855
                     /locus_tag="VUW50_001579"
     CDS             4265..4855
                     /locus_tag="VUW50_001579"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET7983451.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            4852..5931
                     /gene="prfA"
                     /locus_tag="VUW50_001580"
     CDS             4852..5931
                     /gene="prfA"
                     /locus_tag="VUW50_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET7983452.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            5931..6770
                     /gene="prmC"
                     /locus_tag="VUW50_001581"
     CDS             5931..6770
                     /gene="prmC"
                     /locus_tag="VUW50_001581"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET7983453.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASNQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQNRMVVVDD
                     RQD"
     gene            6754..7356
                     /locus_tag="VUW50_001582"
     CDS             6754..7356
                     /locus_tag="VUW50_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HET7983454.1"
                     /translation="MIDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            7358..7789
                     /locus_tag="VUW50_001583"
     CDS             7358..7789
                     /locus_tag="VUW50_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7983455.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSLEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGEGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            7851..9107
                     /locus_tag="VUW50_001584"
     CDS             7851..9107
                     /locus_tag="VUW50_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET7983456.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     AENEAVLEEVRSEVKELTDAFLLYED"
     gene            9115..10089
                     /locus_tag="VUW50_001585"
     CDS             9115..10089
                     /locus_tag="VUW50_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HET7983457.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLTNLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSENLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            10089..10703
                     /gene="pmp23"
                     /locus_tag="VUW50_001586"
     CDS             10089..10703
                     /gene="pmp23"
                     /locus_tag="VUW50_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HET7983458.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     QDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            10777..11751
                     /locus_tag="VUW50_001587"
     CDS             10777..11751
                     /locus_tag="VUW50_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HET7983459.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            11825..13456
                     /gene="rlmD"
                     /locus_tag="VUW50_001588"
     CDS             11825..13456
                     /gene="rlmD"
                     /locus_tag="VUW50_001588"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET7983460.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVATRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMNLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            13670..13903
                     /locus_tag="VUW50_001589"
     CDS             13670..13903
                     /locus_tag="VUW50_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HET7983461.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVLELACWFRVIIVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAVYSIHFNNGYCN"
     gene            14194..14411
                     /locus_tag="VUW50_001590"
                     /pseudo
     CDS             14194..14411
                     /locus_tag="VUW50_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            14804..15829
                     /locus_tag="VUW50_001591"
     CDS             14804..15829
                     /locus_tag="VUW50_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7983462.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            15819..16826
                     /locus_tag="VUW50_001592"
     CDS             15819..16826
                     /locus_tag="VUW50_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET7983463.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            16826..17833
                     /locus_tag="VUW50_001593"
     CDS             16826..17833
                     /locus_tag="VUW50_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET7983464.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFYILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            17833..18627
                     /locus_tag="VUW50_001594"
     CDS             17833..18627
                     /locus_tag="VUW50_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7983465.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVNS"
     gene            19015..19440
                     /locus_tag="VUW50_001595"
     CDS             19015..19440
                     /locus_tag="VUW50_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET7983466.1"
                     /translation="MPKRYLLDGKTPRISATNIDNGILGYYEDIDDKNYRVFENFISV
                     SFLGAVFYHKYKASLDMKIHCLKLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTV
                     LPDIKIKLPIDSRGTPDWNYMERYIEDLKLKCNIANYNI"
     gene            19496..19813
                     /locus_tag="VUW50_001596"
     CDS             19496..19813
                     /locus_tag="VUW50_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HET7983467.1"
                     /translation="MMASKRLSIEEQIEKKEESIKQLQNQKRQLKKKLNEQERKARNK
                     RLIEKGAVFESIFEESIDLTKDEFYKLIKTLNDEEIRLNIMEILEERIDDNVEKSSKD
                     EIT"
     gene            20123..21510
                     /locus_tag="VUW50_001597"
                     /pseudo
     CDS             20123..21510
                     /locus_tag="VUW50_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785367.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
     gene            21836..22009
                     /locus_tag="VUW50_001598"
     CDS             21836..22009
                     /locus_tag="VUW50_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983468.1"
                     /translation="MNKRQELIDELIKANEDGTYKTYKSTEEIKAMNNEEIQIIYSNM
                     KNYLSDKRTHINY"
     gene            22195..22722
                     /locus_tag="VUW50_001599"
     CDS             22195..22722
                     /locus_tag="VUW50_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HET7983469.1"
                     /translation="MINEEISKEAGQAAQTIISYTIKATKESINLEKEIRKKMNETLE
                     KANGNLKSLMGDEMKIKDLYKKGQLENISIDQIDLKDLKKELNKLGVSFSVMKNKESK
                     NYEIFFQAKDIKVMEYAFKQVIAKENKKEKESILKQIKKYKDLSKNKDKTKEKGKRKV
                     KEKVKPNKKDMTREI"
     gene            22849..>22914
                     /locus_tag="VUW50_001600"
     CDS             22849..>22914
                     /locus_tag="VUW50_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HET7983470.1"
                     /translation="MATNKRYYWIKLKEEFFTDKRI"
BASE COUNT         8092 a         3877 c         4491 g         6454 t
ORIGIN      
        1 aaggcgacgc tgacgaagtt tgaagagatt ttcgaagagt ataaagagtt ttggctgttg
       61 acaaaaatga ctctatctag tagaatagta gatgcgatga gtcgataggt agtcttcggg
      121 ctactattga gcataaggag gtcataacgc aggagcggac cttgatgagt tgtgtgaacc
      181 tgctcatcac atgaagatgc ctcttaatcc ctagtctagc gattggggat ttttattttc
      241 caaaaaatga tgaaaaagct ttgcaattca tggaaaaagt agtataatac ttctattata
      301 gaaattttta gaaaattccg aaagaggtta tttatgggat atacagttgc tgtagtcggc
      361 gcgacaggtg ctgtcggtgc tcagatgata aaaatgttgg aagaatcaac acttccaatc
      421 gataaaattc gttaccttgc ttctgcacgt tcagcaggta agtcattgaa atttaaagat
      481 caagatatta cgattgaaga aacgactgaa acagcttttg aaggagttga tattgctctc
      541 ttttcagcag gtggttcgac atcagctaag tatgcaccat acgcagtaaa agctggcgtg
      601 gtagtagtgg ataatacatc ttatttccgt caaaatccag atgttccttt ggttgttcca
      661 gaggtcaatg ctcatgcact tgatgcccac aacggaatca ttgcctgccc taactgttca
      721 acaatccaaa tgatggtggc tcttgagccg gttcgccaaa aatggggctt ggaccgtatc
      781 attgtttcaa cttatcaagc cgtttcaggt gctggtatgg gagcaattct tgagacacaa
      841 cgtgaacttc gtgaggtctt gaatgatgga gtgaaaccac gtgatttgca tgcggaaatc
      901 ttaccttcag gcggtgacaa gaaacattat cctatcgcct tcaatgctct tccacaaatc
      961 gatgtcttca ctgacaatga ttacacttac gaagagatga agatgaccaa ggaaactaag
     1021 aaaattatgg aagatgatag cattgcagta tctgcaacat gtgtgcgtat tccagtcttg
     1081 tcagctcact ctgagtctgt ttatatcgaa acaaaagaag tggctccaat cgaagaagta
     1141 aaagcagcta tcgcagcctt cccaggtgct gttcttgaag atgatgtagc tcatcaaatc
     1201 tatcctcaag ccatcaatgc agttggttcg cgtgatacct ttgttggtcg tatccgtaaa
     1261 gacttggatg cagaaaaagg aattcacatg tgggttgttt cagataacct tctcaaaggt
     1321 gctgcttgga actcagttca gattgctgaa actcttcatg aacgtggatt ggttcgtcca
     1381 acagccgaat tgaaatttga attaaaatag tcatatcgtt taggagttca gatgaactcc
     1441 ttctttgaaa tagagaggtg ttttcgtgtc ttatcaagat ttaaaagaat gtaaaatcat
     1501 tacagccttt attaccccct tccatgagga tggttccatt aactttgatg ctattccagc
     1561 cttgattgag catttattgg cccatcatac ggatggaatt cttctcgcag gaacgactgc
     1621 tgagagtcca actttgaccc acgatgagga gttggagttg tttgcggctg tacaaaaggt
     1681 tgtcaatgga cgcgttcctt tgattgcggg tgtaggtact aatgatacgc gtgactctat
     1741 tgagtttgtc aaagaagtag cggaatttgg tggtttcgca gctgggcttg ctattgttcc
     1801 ttactacaac aaaccttctc aagaagggat gtatcagcac tttaaggcta ttgcagatgc
     1861 ttctgaccta ccaattatta tctataacat tccagggcgt gtagttgtcg aattgactcc
     1921 agaaaccatg cttcgcttgg ctgaccatcc aaatattatc ggtgtcaaag aatgtactag
     1981 cttggctaat atggcttact tgattgagca caagcctgaa gagttcttga tttatacagg
     2041 tgaggatgga gatgctttcc atgccatgaa ccttggggcg gatggggtta tttctgttgc
     2101 ctctcataca aatggggatg aaatgcacga gatgtttact gccattgcag aaagcgatat
     2161 gaagaaagcc gcagcaattc agcgtaaatt cattcctaag gttaatgctc tcttctctta
     2221 tccaagtcct gctccagtta aggcaattct taactatatg ggatttgaag ctggacccac
     2281 tcgtctacct cttgttccag caccagaaga agatgccaaa cgcattatca aggttgtcgt
     2341 agatggcgac tacgaagcaa ctaaggcaac tgtaacaggg gtcttaagac cagattacta
     2401 ataaagacaa taaaatccat gaccgaatga aggatcatgg atttttctta ttttcctaaa
     2461 cagaattggc taaagagttg ggtaataagt tcatctggag cagcatcccc agtgatttct
     2521 ccgaggattt cccaagtacg agtcaagtca acttgaagca aatcaactgg catccccagc
     2581 tcaagacctt gattaacggc ttgtaggctt tcaactgctt tttcaatcag ggaaatgtga
     2641 cgggcgtttg acaagtaagt agcatcttgc tcgaccaagc cagcattttc aaagaagagg
     2701 ttgttgattc gctcttcaat cttgtcgatg ttttggtttt taaggactga aatacggata
     2761 acgtcttcag gtagtttcga agtttcaatc gtttctggca ggtcggtttt attaagtaga
     2821 ataatgcgat tggtatcttg gctaatttca agaagttgtc tgtcttgcgc agtcagtggt
     2881 tcactggcat ttagcactag tagaaccaag tcggcttcct tgagggcttt tttcgaacgc
     2941 tcaacaccga tttgttccac tatgtcatct gtttcacgaa taccggctgt atcaatcaat
     3001 ttgagaggta caccattgat gttgacgtac tcttcgatga catcacgtgt ggtaccagca
     3061 atatctgtaa cgatagcctt gtcctcacgc aagagattgt tgaggaggct agatttccca
     3121 acgttgggac gtccgatgat ggcagtggaa attccttcac gaaggatttt accgcgacgg
     3181 gctgtcctaa ggagattggt tagcaattgt tcaaattcca tagttttctc gcggacaaca
     3241 gcagtagtcg cttcctcaac atcatcatac tcaggatagt cgatattaac ttcgacttgg
     3301 gcaagtgtat tgaggatttc ttgacgggta ttgttaatga ggtcagaaag agaaccatct
     3361 agttgtttga ccgcaatgtt catggcttta tctgtcttag cacggatgat atccattact
     3421 gcctcagcct gagtcaagtc aacacgtcca tttaggaagg cacgtttagt aaattcacca
     3481 ggttcagcca atcgagctcc ttcacggata gctagctgga gaatttcatt agtcaccgca
     3541 atcccaccgt gggtgttaat ctcgataata tcttcacgag tgaaggtttt tggagatttc
     3601 atagccccaa tcatgacctc gtccatgact ttaccagtca gagggtcaac gatgtgaccg
     3661 tagttaagtg tatggctagc aacctgactc aagtcttttc ctttgaaaat cttttgcgca
     3721 atagcaaaac tgtttgttcc gctcaggcgg acaataccaa tagccccttc acctagtgga
     3781 gtagagatag cagcgatggt atcaaattca cgtgtaatca taatgtttcc tttaataaat
     3841 agctcttttc ttggaaattt ttccaagtat cctttcaaat tgtaacaaat ttaggctatt
     3901 tcttcaatgg atgttacttg gagattgaaa aagcctaagc aattctgctt aagctagctt
     3961 atttggtacg catttcccct tgtgggaagt aagttccttc tggcatgtcg ttgatgatga
     4021 catggacagc agattggggg gctccagtat tacggacaat agcttccgtt acttccttag
     4081 caagagcttt cttttgctcg agcgtgcgtc cttcaaataa atcgatgcgt acaaatggca
     4141 taatagcttc ctccactagt ttttgatttc ttctatttta ccacattttg ccgtttaaag
     4201 cttcagaaaa ttatgatata ctagaatgta gcaaaaattt agaaatggac gtgaagcaag
     4261 aaacatggca cagttgtatt atcgttatgg gaccatgaac tctggtaaga cgattgagat
     4321 tctcaaagtg gcctataact acgaggagca aggaaaaggt gttgtcatta tgacttcggc
     4381 tctggatacg cgtgacggtg ttggctatgt gtcgagtcga attggcatga aacgccctgc
     4441 ccttgcgatt gaggaaacaa cggatatctt tggctatatc cgagacctac cagaaaaacc
     4501 ttactgtgtt ttggtcgatg aagcccagtt tctcaaacgc caccatgttt acgacctagc
     4561 tcgtgttgta gacgagttag acatacccgt catggctttt ggcttgaaaa atgactttcg
     4621 caatgaattg ttcgaaggtt ccaaatatct cttgctttta gcggacaaga ttgacgaaat
     4681 caagaccatc tgtcagtatt gcaagaaaaa ggcgaccatg gtgttgcgaa cacaggatgg
     4741 actgcccgtt tacgatggag aacagatcca gattggtggt aatgaaacct atatctcggt
     4801 ttgccgtaaa cattattttg cccctgaaat caataaggag aatgaagaaa aatgaacatc
     4861 tatgatcaac tacaagctgt agaagaccgt tatgaagaac taggagaatt gctgagtgac
     4921 cctgatgtcg tttcagacac caagcgtttt atggagcttt caaaagaaga agcctccaat
     4981 cgtgacaccg taatagccta ccgtgagtat aaacaagtcc ttcaaaatat cgtcgatgcc
     5041 gaagagatga ttaaggaatc aggtggagat gcggacttgg aagaaatggc caagcaagaa
     5101 ctcaaagatg ccaaggctga aaaagaagaa tatgaagaaa aactgaaaat tttgctcctt
     5161 ccaaaggatc caaacgatga caagaatatc atccttgaaa tccgtggagc agctggtgga
     5221 gacgaagcgg cacttttcgc tggagatttg ctaactatgt accaaaagta tgctgaagcc
     5281 caaggctggc gctttgaagt catggaggct tctatgaatg gtgtcggtgg tttcaaagaa
     5341 gtggttgcta tggtttcagg tcagtctgta tactctaagc ttaagtatga atcaggtgcc
     5401 caccgtgtgc aacgtgttcc tgtgacagaa agccaaggcc gtgttcatac ttcgacagcg
     5461 acagttcttg ttatgccaga agttgaagag gttgaatacg acattgatcc aaaagacctt
     5521 cgtgtcgaca tctatcacgc ctctggtgct ggtggacaga acgtcaataa ggttgcgact
     5581 gccgtgcgta tcgttcactt gccaaccaat atcaaggttg agatgcagga agaacgtacc
     5641 cagcagaaga accgcgagaa ggctatgaag attatccgtg cacgcgtcgc tgaccacttt
     5701 gctcagattg ctcaggatga acaagacgct gagcgtaagt cgacaatcgg tactggtgac
     5761 cgttcagaac ggatccgaac ttataacttc ccacaaaacc gtgtcacaga ccaccgtatc
     5821 ggcttgaccc tccaaaaact agatacgatt ttgtctggta aattggacga agttgtggat
     5881 gccctggtgc tttatgacca aacacaaaaa ctagaagaat taaacaaata atgaaattag
     5941 ctcaattatt ttcaaatttt gaagaagagt tgataagaca aggagaggaa gcggaaagcc
     6001 tctcttttgt ctatcgtagt ctgaaaaatc tatcttttac agactttatt tttgccctcc
     6061 agcaggaggt gacaacagag gaagaaaaac aatttgtaga agatatttac cagcaactag
     6121 cagctcacaa gccagctcag tacatcattg gacaggcaga tttttatgga atgcatttaa
     6181 aggtggatga acgggttttg attcctcgtc cagaaacaga ggagttagtg gagcttattc
     6241 taactgaaaa tcttgagacg aatctctcag ttctggatat tggaacaggt agtggagcta
     6301 ttgctctcgc tctagcaaaa aatagaccag attggtcagt gacagcagca gatgtttctc
     6361 aagaggcctt agagcttgca tcagagaatg ctagcaatca aaatcttaat atatttttta
     6421 aaaaatctga ttgttttgca gaaatttctg aaaaatatga tataattgta tccaatccac
     6481 cctatatctc tcgtgaagat gagtcagagg taggcttgaa tgttttgcat tcggagcctc
     6541 atctagctct ctttgcagac gaggatggcc tagctattta ctgtagaatt gcggaagatg
     6601 caaaagacta tctcaaagat ggtggtaaga tttaccttga aattggatac aagcaaggtc
     6661 aaagtgttcc cgaacttttt aggaaacatc ttcctgaaaa aagagttcga acactcaagg
     6721 accaatttgg tcaaaatagg atggtcgtgg ttgatgatag acaggattag acaagagttg
     6781 gaaaatggtg gagctgtcgt tctacctaca gagactgttt atggtctttt tgccaaggcc
     6841 ttagatgaaa aagcagttga ccatgtttac caactcaaac gtcgtcctag agataaggca
     6901 ctcaatctca atatcgcctc tttcgaggat atcttgcact tttctaagaa tcagccaact
     6961 tatctacaaa aacttgtaga gacctttttg ccaggtccct tgaccattat tctcgaagcc
     7021 aatgaccgag ttccctattg ggtaaattct gaccttgcaa ctattggatt tcggatgccc
     7081 agtcacccta tcacactgga tttaattcga gagacaggtc ccttgattgg gccgtctgcc
     7141 aatatctcag gtcaggcaag tggtgtaacc tttgaacaaa ttctgaagga ttttgaccaa
     7201 gaggttctgg gtctggaaga cgatgctttt ctaactggac aggattcaac tattgtggat
     7261 ttgtctggag acaaggtgaa aatcttacgc caaggcgcaa ttaaacgaga agatattctt
     7321 gctcggttgc cagagatttc ttttgaggag gcttgaaatg ctaagagatt tgcaagaaac
     7381 agatgtgaaa gcgatatgtg acatcaacca agaggctttg ggttatactt ttagtctaga
     7441 ggaaacggct agccaactag ctagactgtc tcaggattcc catcatttcc tacttggcta
     7501 tgaggatgca gctaatcatg tcttacttgg atatgtccac gctgaagttt acgaatcact
     7561 ctattccaaa gcaggattta atatcttagc tttagcagtt tcacctcaag cgcaaggtga
     7621 aggtatcggt aaaagtttac tacaagggtt ggaacaagaa gccaaaagat gtggttatgg
     7681 gtttatccgc ttaaattctg ccaatcatcg tctgggtgct catgcatttt atgaaaaagt
     7741 tggctatact tgtgataaaa tgcagaaacg gtttattcgc atcttttagt ttgattttct
     7801 tattgtaaaa tcaaactaat ggactagtca cacaataaag gagaagacct atgatttttg
     7861 acaaagatga ttttaaagca tatgatgctg atctctggaa tgctattgcc aaagaagaag
     7921 aacgccaaca aaataatatc gagttaattg cttcggaaaa cgtagtttcc aaggctgtta
     7981 tggcagctca agggtctatc ttgacaaata aatatgccga gggttaccca ggacgccgtt
     8041 attatggtgg aactgatgta gtagacgttg tagagactct tgctattgaa cgcgcaaaag
     8101 aaattttcgg tgctaaattt gccaatgtcc aaccacattc aggaagccaa gctaactgtg
     8161 cggcttacat gtccttgatt gagccaggtg atacggttat gggaatggat ttggcatcag
     8221 gtggtcattt gactcatggg gctcctgtta gcttctctgg tcaaacctac aactttgttt
     8281 cttatagtgt tgatcctgaa acggaactct tagactttga tgctatcttg aaacaagccc
     8341 aagaagtaaa accaaaactg attgtagctg gtgcttcagc atattctcaa attatcgatt
     8401 tttcaaaatt ccgtgaaatc gcagatgctg tcggtgcgaa gctcatggtg gacatggccc
     8461 atatcgctgg tttggttgcg gctggccttc atccaagccc agttccatac gctcatatca
     8521 caacaacaac gacccacaaa acccttcgtg gtcctcgtgg tggtttgatt ttgaccaatg
     8581 acgaagaact tgctaaaaaa atcaattcag ctattttccc aggtattcag ggcggtcctt
     8641 tagagcatgt tgtggcggct aaggcagttt ccttcaaaga agttttggat ccagccttca
     8701 aggaatatgc tgccaatgta attaagaaca gcaaggctat ggcagatgtc ttcttgcaag
     8761 accctgattt ccgtattatt tcaggtggaa ctgaaaacca tctcttcctt gttgatgtga
     8821 ctaaggttgt agaaaacgga aaagttgctc aaaacttgtt ggatgaagtc aatattacct
     8881 taaataaaaa ctcaatccct tacgaaagct tgtcaccatt caagacaagt gggattcgta
     8941 tcggagcagc agccattact gcacgtggat ttggtgaaga agaaagtcgc aaagtggctg
     9001 aactcatcat taaaaccctt aaaaatgcag aaaatgaggc tgtcttagaa gaagtgagaa
     9061 gtgaagttaa agaattgaca gatgccttcc tactatacga ggactaaaac ttttatggac
     9121 atttatatta agaaagccat tattcaccag ttcagtccgg atgataccga actgttctta
     9181 gcagataagt ttctcaatat tactccaaaa atcgaagaat acctgcgtaa aaaaattgaa
     9241 catgtgtatt cagatgaagc caagactggg attttcgaag aagaaaatcc cttcttcaat
     9301 catattacag acgatttgtt ggagacatca gtaacactga ctaatctctg gaaagaggag
     9361 ttcagtattt ctgaaaatct aaaaactaat gacttgattt ttgtgcaatt ttctaaagaa
     9421 ggtgtagaac atttcgcttt cttgcgaatt gccctgcgtg agaccttgac ccatctcggt
     9481 ggagaagttg ataatccaat caagctgact cagaataacc tgcctggatt tggaacgggt
     9541 gctgacgagg ccttggtggt caatcttcag agtcgcaagt accatctgat tgaaaaacga
     9601 atcaagtaca acgggacttt tttgaactat ttttcagaaa atctcctagc tgttgcccct
     9661 aagatttcac ctaagaaatc catcaaggaa ctggaaaaaa cagcccagag aattgccgaa
     9721 tcttttaaca cagatgattt tcaatttcaa tccaaggtca aatcagctat ttttaacaac
     9781 ctagaagaaa gcaatgaatt gtcacctgag aaattggcta atgacctttt tgacaacaat
     9841 ctgacggctc gtttgagctt tattgaccaa gtcaaagaag ccgtaccaga acctgttcaa
     9901 tttgatgaaa ttgatgccag tcgccaatta aagaaatttg aaaaccaaaa actctcctta
     9961 tcaaatggaa ttgagctcat cgttcccaat aacgtctatc aagacgccga gtctgttgag
    10021 tttatccaaa acgaaaatgg aacctactct atcttaatca aaaatatcga ggatatccaa
    10081 agtaaataat gtttaaacga attcgaagag tgcttgtact agcagtcttc ctttttgctg
    10141 gctataaagc ttaccgcgtt catcaagatg tcaaacaagt catgacctat caacccatgg
    10201 tgcgagaaat cttgagtgaa caagacaccc cagcaaacga agagcttgtg cttgctatga
    10261 tttatactga aacaaaagga aaagaaggcg atgttatgca gtctagtgag tctgcaagtg
    10321 gttccaccaa caccatcaat gataatgcct ctagcattcg gcaaggcatt caaactctga
    10381 caggcaatct ctatctggcg cagaagaagg gtgtagatat ctggacagct gttcaagcct
    10441 ataattttgg acctgcctat atcgatttta tcgcccaaaa tggcaaggaa aataccttgg
    10501 ctctagccaa acagtactct cgtgagactg ttgccccctt gcttggtaat acgactggaa
    10561 agacttatag ttatattcat cccatttcca tttttcacgg tgctgaactc tatgtaaatg
    10621 gaggaaacta ttattattct agacaggtac gacttaacct ttacatcatc aaatgtttca
    10681 ctctcttttc aacatctggc tagaccaggt gtttttgtta taagttttct ttaagataga
    10741 tatattactc taaagaagta aaggaggaaa ttccctatga gaaagaaact ctttctgact
    10801 agtgctgcgg tcttgtgggc agtaacagct atgaatagcg tccatgcagc aacagatgtt
    10861 caaaaagtta tcgatgaaac ctatgtccaa cctgaatatg tcctaggttc ctccctatct
    10921 gaagaccaaa aaaatcaaac tcttaaaaaa ctgggctaca atgcctcaac agataccaaa
    10981 gaactcaaga ccatgacacc tgatgtttat tctaaaatca tgaatgtggc caatgactct
    11041 agcttacagt tgtattcatc agccaagatt caaaagctag gtgacaaatc gccacttgag
    11101 gtcaagattg aaacacctga aaacatcact aaggtaactc aggatatgta ccgaaatgca
    11161 gccgtaactt tgggtgtgga acacgccaaa atcaccgtag cagcacccat tccagttaca
    11221 ggtgagagtg ctttggctgg gatctactat tcgctagaag ctaatggagc caaggtgccg
    11281 caagccaata aagatttggc tcaagaagag ctaaaggctt tgtcagatat caatgctgaa
    11341 aacaaggaca aatcaggcta tgatgctaat aaattaaacg ttgccctagc tgatatcaag
    11401 tcaggactcg ccaaagctaa agaaagcaag ggaaatctga cagaagaaga tatccgcaag
    11461 attgttgaag ataccttaaa aaattacaaa cttgatcagg tcataacagg aaaccagatc
    11521 aatatcatca tcaattttgc cttgaatctc tcaaagagtg atatcctcag caatgcagat
    11581 ttcactaaaa ccctaaatga ccttaaacaa agcatcgtat cacaagctgg cgacagtttt
    11641 aaaaatatca accttaactt tgatgcggat aaggcgctag aggacggtgg taacttctta
    11701 agctccctct ggcaagccct tgtcaacttc ttcaagagtt ttggttctta agaaatttca
    11761 tggtataatg gatggtaacc gtaacgttgc cgtctttttt gtcggccaat agaaagagaa
    11821 aagaatgtta aagaaaaatg atattgtaga agttgaaatt gttgatttga cccatgaagg
    11881 ggcaggagtt gccaaggtag atggtttggt cttttttgta gagaatgctt taccgagtga
    11941 aaaaattctc atgcgtgtcc tcaaggtcaa taaaaagatt ggctttggaa aagttgaaaa
    12001 ataccttgtc cagtcaccac accgtaatca agatctagat ttggcttacc tgcgttcagg
    12061 aatcgcggat ttaggacacc tttcttatcc agaacagctc aagtttaaaa ccaagcaagt
    12121 caaggacagt ctctacaaga ttgctggaat tgcagatgta gaagttgctg aaacgcttgg
    12181 tatggaacat ccagtcaagt atcgcaataa ggcgcaggtg cccgttcgtc gagtgaatgg
    12241 tgtcttggaa acaggatttt tccgtaagaa ttcgcacaac ctcatgcccc ttgaagattt
    12301 ctttatccag gatcctgtca ttgaccaagt cgtagtagct cttcgagacc tgctccgtcg
    12361 ttttgattta aaaccttatg acgaaaagga acagtctgga ttgattcgga atcttgtggt
    12421 gcgtcgtggt cactattcag gacaaatcat ggtcgttttg gtggcaactc gtccaaaagt
    12481 ttttcgtgtt gaccaattga ttgaacaagt tatcaagcag ttcccagaga ttgtgtctgt
    12541 catgcaaaat atcaacgacc agaataccaa tgcgattttt ggtaaggagt ggcgcactct
    12601 ttatggtcaa gactatatta cggaccagat gttgggaaat gacttccaaa tcgctggccc
    12661 agccttttac caagtcaata ctgaaatggc ggagaaactc tatcaaacag ccattgactt
    12721 tgcagagtta aaaaaagatg acgtggttat tgatgcttat tctggtattg gaaccattgg
    12781 tttatcagtc gccaagcatg tcaaagaagt ctacggtgtt gaactgattc cagaagcggt
    12841 tgagaatagt aaaaaaaatg ctcagttgaa caatatttca aacgcccact atgtctgtga
    12901 cacagctgaa aatgctatga agaattggct taaagatggg attcaaccaa ccgttatctt
    12961 ggttgatcct ccacgcaagg gcttgacaga aagctttatc aaagcaagcg cccaaacagg
    13021 agccgatcgc atcgcctata tctcctgcaa tgtcgcaacc atggcgcgtg atatcaaact
    13081 ctaccaagaa ttgggatatg aattgaagaa agtccagccg gtggatctat ttcctcaaac
    13141 gcatcacgtc gagacggtag cacttttgtc caaactcgat gtcgataagc acataagtgt
    13201 tgaaattgag ctggatgaga tgaatttgac aagtgcggag agcaaagcaa catatgctca
    13261 aatcaaagaa tatgtttgga ataaatttga attaaaagtt tcgacattat atattgcaca
    13321 gataaaaaag aaatgtggaa tagaattacg agaacattac aacaagtcta aaaaggataa
    13381 acaaattatt ccacagtgta cacctgaaaa agaagaagcc atcatggatg ctttgagaca
    13441 cttcaaaatg atttaataga aaagaatgac agtatatgac tttctgcatt tattacattc
    13501 ctacttggta taggaacagc tattattcct ttcttgcaag gtatcaatta gaaaataggc
    13561 tcaatataaa gattgatagg atcattttta tatttaaagg agtgttgaaa tgattgataa
    13621 aggcaacaaa aaattttagg ataaatttgc taagttgtat gcccctttta tgaaaaaaga
    13681 taaagaggtt tatgataaag tttgtgaata tcttagtcct catttgaata aagatatgga
    13741 ggtgcttgaa cttgcttgtt ggtttcgtgt cataatagtt atagaggcaa atagttatgt
    13801 aaatataagg agttcaagac ttctaccaaa gtttaaaact caaaaaataa atagttggtg
    13861 tgctgtttat agtatccatt ttaataatgg atattgtaat tagcatacca atgaatttaa
    13921 agattcttta aagagtctta ttttgtgatg aaaatttaat atgtaaatct cagacgatag
    13981 aaattaaaaa ctctgtcgtc ttttttatac tcaaaattag gaggtaaaaa tggtaaggat
    14041 aagaggtccc acttaaaaca atttatggca aaataaggac ggaataacac aacaaattct
    14101 ctaaaacaaa tcactaaatc aatgtaagat tgaatgaaat caatatttat gctataatta
    14161 aataaattta atgaagaaaa aaagagggat attatggcac ttaactataa accattatgg
    14221 atacagttag caaaaaaaga ctaaagaaaa cagatgtaat agctatggca ggacttacaa
    14281 caaatgttat ggcacaaatg ggaaaggata aaccaattac atttaagaat ttagaaagaa
    14341 tatgtaaggc tttatcttgc actcctaatg atattattag ttttgaagat aattttagtg
    14401 acgaggaata gaaaatgact ttaaggacag aagatcaagt tagggattat gcaagagaag
    14461 tataggcttt aatgaagttg aagaaaacat caatcaaggt actggtcaaa taactacttt
    14521 taatcaatta ggcttcaagg gatattcaga taagccagat ggttggtatt tacctaaaaa
    14581 tatgaatgat gtagcaataa tccttgaaac aaaatcagaa gaaagagata ttagcaaaca
    14641 aatttttatt gatgagttaa tgaaaaatat agacataatt taactaaaaa taaaaactag
    14701 atcctttttt gaaaaaatta tattattaaa tttgtaactg tatctattga caatgataat
    14761 tattatcgat acaatagact tgaaatatgt ttaaggagtt tttatgaaaa acaaattttt
    14821 tctaatagct attttagcta tgtgtatagt ttttagtgct tgttcttcta attctgttaa
    14881 aaatgaagaa aatacttcta aagagcatgc gcctgataaa atagttttag atcatgcttt
    14941 cggtcaaact atattagata aaaaacctga aagagttgca actattgctt ggggaaatca
    15001 tgatgtagca ttagctttag gaatagttcc tgttggattt tcaaaagcaa attacggtgt
    15061 aagtgctgat aaaggagttt taccatggac agaagaaaaa atcaaagaac taaatggtaa
    15121 agctaaccta tttgacgatt tggatggact taactttgaa gcaatatcaa attctaaacc
    15181 agatgttatc ttagcaggtt attctggtat aactaaagaa gattatgaca ctctatcaaa
    15241 aattgctcct gtagcagcat acaaatctaa accttggcaa actttatgga gagatatgat
    15301 taaaattgat tcaaaagcct taggtatgga aaaagaaggt gatgagttaa tcaaaaatac
    15361 tgaagctcgt atatccaaag aattagaaaa acatccagaa atcaaaggaa aaatcaaagg
    15421 aaaaaaagta ttatttacta tgattaatgc tgcagataca tcaaaattct ggatttatac
    15481 tagcaaagat ccaagagcaa attatttaac agatttaggt ctagttttcc ctgaatcatt
    15541 aaaagaattt gagagtgaag atagttttgc aaaggaaatt tctgcagaag aagcaaataa
    15601 gataaatgat gctgatgtaa tcataactta tggtgatgat aaaactcttg aagctttaca
    15661 aaaagatcct cttttaggta aaataaatgc aattaaaaat ggtgccgttg ctgtaattcc
    15721 agataataca ccgttagcag cctcatgcac tccaacacca ctttcaataa actatactat
    15781 tgaagaatat ctaaatcttt taggaaatgc atgcaaaaat gcgaaataaa aaacaaataa
    15841 acctaggcat aatttttgta atctgcctag gtcttcttat tacaatattt ttgtcattaa
    15901 agcttggaac aaaagaaatt aatatcagag attttttagc agcttttgga atgggtaata
    15961 caaatgatga ttttattaaa tcaattatat ataaaagaat acctagaact atttttgcaa
    16021 ttttagcagg ttctagtctt gccataagcg gtgtattgat gcaatcagtt actagaaacc
    16081 caatagctga tccaggtata ctcggtataa acacaggagc aagtcttagt gtagtaattg
    16141 gtctttcttt tttaggaatt tcatcaagca taagccatat aagttttgca atcattggtg
    16201 gcttagtaag tgcaattttt gtatacgcga ttgctgtaag cggaaaagca ggccttaccc
    16261 ctataaaact tgccttatca ggaacttgtg ttagtatggc tttaagcagt tttgtaagtt
    16321 ttttaatttt accgaataat aacgtcttag acaaatttag attttggcaa ataggtagcc
    16381 ttggagcagc tacattatct tctatatcta cactactacc ttttataatt ttaggtcact
    16441 tgatagctat atttatttca tcagatttaa acgctttagc tatgggtgat gaaatggctg
    16501 ttggtcttgg agttaatgtt aataggataa gatcacttgc aataattgca agtgtgcttt
    16561 tatgttcaag tattactgca attggtggac ctattggctt cgtaggtctt atagttcctc
    16621 acttttgtgg cttatttata agcaaagata tacgcacaat gaccatttct tcatctttta
    16681 taggtgcaga gctcttgctt atatgtgata taatcggccg tatgttaggt aaaccaggtg
    16741 aaattgaagt agggataatt actgcaataa tcgggggtcc agtacttatt tatgtaacta
    16801 tgaaaaatag aggggttaat aactaatgca aaatttaatt ataggtattc aaaaaagaaa
    16861 aaatagaata acactattct cctcactatt tttattaata ataatcagtc tatcattttt
    16921 cattttactt atcggagatg aaagttattc tttttcaact ttgattaaag tcttaaatag
    16981 tgaaactgtt cctggagcta gtttttcgat tatggaaatt agattaccaa aattattagc
    17041 aggaattata gctggctggt cttttggatt ggcaggattt atctttcaaa ctatgttaag
    17101 aaatcctctt gcaagtcctg atataatcgg tgtcacaagt tcttcatcta ttgcagcggt
    17161 cttttacata ttggtattaa aaacaaatag tttaactact ggaattattt caataacttg
    17221 tggactaaca tcatctttaa tattattttt actagctaaa aaagatggtt tttcagcagc
    17281 aagactgata atattaggta ttggttttca agctgtcaca agagcaggca cctcattttt
    17341 attgttgaaa gtagcaagat atgaattaca agaagttatg agatggctca gtggctcttt
    17401 atcttttaca aagttagatg acatacctct tgttctaata gtaagtatta ttgctactat
    17461 attagtttta ttttttaata aaagactaga aattattgaa cttggtgaag aaatagcaat
    17521 cggacttggt gcaaatcccg agctttcaag gcttgtttta attttttgcg ctgtatcttt
    17581 aactgctttt tctacttcaa ttacaggacc aatagcttgt atatcttttt tagctggtcc
    17641 catagcctta aatattggca agaaaagaag cccaatatta gctggattgg ttggaatttt
    17701 actagttttg ttatcagaca tattctctca aaatatttta ccagctagat atccagtagg
    17761 tgttgtaact ggcttgttag gttcaccata cttaatatat ttactaataa aaatgaacag
    17821 gaggaatata taatgaaagg tctgtggtca aataatctta cctgcggtta tgatgaaaaa
    17881 ataatcttgg aaaatataaa tataaaaata cctgaagaaa aaatatcagt tattattggg
    17941 tcaaatggtt gtgggaaatc aacactcatt aaaaccttgt ctcgacttat aaagccatta
    18001 gagggagaag tattgcttga taataaatca attaattctt ataaagaaaa agatttagca
    18061 aaacacatag ctatattacc tcaatctcca ataatccctg aatcaataac agtagctgat
    18121 cttgtaagcc gtggtcgttt cccctacaga aagcctttta agagtcttgg aaaagatgac
    18181 cttgaaataa taaacagatc aatggttaag gccaatgttg aagatctagc aaataaccta
    18241 gttgaagaac tttctggggg tcaaaggcaa agagtatgga tagctctagc cctagcccaa
    18301 gatacaagta tcctactttt agatgagcca actacttact tggatatctc atatcaaata
    18361 gaactattag acctcttgac tgatctaaac caaaaatata agacaaccat ttgcatgatt
    18421 ttgcacgata taaatctaac agcaagatac gctgattacc tatttgcaat taaagaaggt
    18481 aaacttgttg cagagggaaa gcctgaagat atactaaatg ataaactagt taaagatatc
    18541 tttaatcttg aagcaaaaat tatacgtgac cctatttcca attcgcctct aatgattcct
    18601 attggcaagc accatgttaa ctcttaataa ttgaatattg gaattcattt aaaagtcgca
    18661 aattttgaac tgaccctcaa aagttaggcc aaaaaactaa ctaaaggagg tcagtttttt
    18721 aatggcaaat acagcacaga atttaagata aaattagtaa aagaatatca tgaatgaaaa
    18781 ataggatata gagaataaca gcaatattaa aaagatcagg actgataata aacaaaaaga
    18841 aagtacaacg actagttcaa aagctaaaac ttcaagtaaa aagttattca agaaaatcta
    18901 gaaaatactc atcctacaaa ggacaggtag gaaaaatatc agacaacaaa ataaaaagaa
    18961 atatttatta ccgaaaaaaa gggaaaatcg agtcaagtac caacaggctc atctattcca
    19021 aaaagatatt tactcgatgg aaaaactcct agaattagtg ctacaaatat tgacaatggt
    19081 atcttaggtt actacgaaga tatagatgat aaaaattata gagtttttga aaattttata
    19141 tctgtttcat ttttaggagc agtcttttat cataaatata aagcaagcct agatatgaaa
    19201 attcattgtt taaaattaaa aaataaagaa ttgaataaag aagtagcatt ctatttaact
    19261 tcaattatta ggcaagcttt aaaaaatact gaatataaag atcaaatatc tagcacagta
    19321 ttgccagata tcaaaattaa attaccgata gacagtagag gtactccaga ttggaattac
    19381 atggaaagat atatagaaga cttaaaatta aaatgcaata tagcaaatta taatatttaa
    19441 aataagaacg attagaaata aaaatctagt cgttttttat tttaagaaag gatacatgat
    19501 ggcaagtaaa agattaagta ttgaagaaca gattgaaaag aaagaagaaa gcattaagca
    19561 attacaaaat caaaaaagac agttgaagaa aaaacttaat gaacaagaaa gaaaagcaag
    19621 aaacaaaaga cttatagaaa aaggtgcagt gtttgaaagt atttttgaag aaagtattga
    19681 tttaacaaaa gatgaatttt ataagttaat aaaaacgtta aatgatgaag aaataagatt
    19741 aaacataatg gaaattttag aagaaagaat agatgataat gtagaaaaat catctaaaga
    19801 tgaaataaca taattgtccc ttggttacaa gggcgcaatt atacacccta aagggtgctt
    19861 gcgtcctgcg gagtcaaacc cttgcaggga gcaacaacca ttcgcttttt aaaagtcaag
    19921 ggtaaataaa ctcgctaagg ctcgcccttg acttttaaaa tttgaaatga aagtaacaat
    19981 taagccgaca tactgaaaca acaaaaatta attcagtagt cggctttttt tattctatca
    20041 tttcaaaacg ctcattcaca aagaggatat aaaaaaacta ttaagcctcc acctaaaaca
    20101 acaaagaaag gaagtgataa aaatggcaga cagttttcat ttttcagtaa acataatatc
    20161 aagaggaaaa ggcaaaagtg cagtagcaag tgcagcatat ataagtggag aaaaaataaa
    20221 aaatgaatgg gacggagtaa cccatgacta cacaagaaaa gaaaaaatat tagtaaaaaa
    20281 tataatattg cctgatcata taccaaaaga atttaatgat aggtctacct tatggaataa
    20341 agtagaaatg gcagaaaaaa attctaatgc acaactagca agacaattca taataggact
    20401 accaaaagag ttatctttaa gtgaaaataa aaacctggtt gaaagatata taaaagaaaa
    20461 tttaacatca caaggaatga tagtagatta tgcaattcat gatgagagtc aagataaaaa
    20521 tggaaatatc cattgtcata taatgaccat aatgcgacca ataaacgaaa aaggagagtt
    20581 cttagccaag tcaaaaaaag aatatatcct agatgaaaaa ggagaaaaag tattaaacaa
    20641 aaatggaaaa ccaaagacaa gaaaagtaga actaacaacc tggaacgata caggcaatgt
    20701 agaacaatgg agagaaaatt tttcagatct ttgtaacaaa tatttagaaa gagcaggagc
    20761 agaaaaaaga gtagaccata ggagttttaa aagacaaaat tcagattatc taccgacaat
    20821 ccatttagga tcagcagcaa gtgcaatgga aagaaaggga atagaaactg acaagggaaa
    20881 ctataataga gaaataagaa aatataatca acttgtaaaa acaataaaag aagagataaa
    20941 aacattaaag ggttggatag gaaatttatt agataactta tctactgctt atgaaaaatt
    21001 taaggatatc gaaagagata aggtaataga caaccctaaa ctttttaatc taacaaatta
    21061 tctattaact tattcagaaa ttcaaaaaga aaaaagtaaa tatctaaaag gatatgcaaa
    21121 aactaataaa gaaaaatatg acttcaaaaa gctaacaagt gcatatagtt atttaagaaa
    21181 aaacaacata gaaaccatag gtcagttaca aacaaaaata gaaactttaa agtctaatag
    21241 ttataggcta aataaaaaag caaagacaat ccataaagaa atggaagatg tagaaaagaa
    21301 aattctatac tatgaaatct acaaagccaa aaaagaagtt tatgaagaat atcaaaagaa
    21361 aaatatattc acaaaagaag cattttataa caaacataag aaagatatag accaatataa
    21421 agtagtaagt gggaaattaa aaaactccta tcagataagg aaaaactaag tcctaaaaag
    21481 tggaatgaag aaaaaattct tttaatgtaa aatcttgaag aaataaataa agaaaaagac
    21541 aagataaaag atgaatacca ggaaattaat catataaaat attcagtaga ttttgtaaac
    21601 aaagaacttg gtatagattt atccatagaa atagataagc taataaaaca gggagaaaaa
    21661 ccaagtgtaa tagcacagat taaaaagttc caagaccaag tcaataaaga taatgaatac
    21721 agagaaatga tgaaaaacaa gaaaatggat caagaaagat aagacctggt aaaaatatct
    21781 tatccaagct ataatatgac caaacaacta aagtcagatc taggaggtaa tagagatgaa
    21841 taaaaggcaa gagctaatag atgaactcat aaaagcaaat gaagatggaa catacaaaac
    21901 ctataaaagc acagaagaaa taaaagcaat gaacaatgaa gaaatacaga ttatatattc
    21961 caacatgaaa aactatctat cagacaaaag aacacacata aactattaaa ggtggaaagg
    22021 tagtgtaagc tatttttaaa agtataaaag gtacaaagac ctaagaaata tataaaaaca
    22081 tcaaaataaa gtattccacc accaagaaca ataaaataaa aacaagcaaa gggaagtata
    22141 gaaaaattaa aacctatact tccctttttt aattcaaaca aaggagagaa aagcatgata
    22201 aacgaagaaa taagcaagga agcaggtcaa gcagcacaaa ccataatatc atacacaata
    22261 aaggcaacaa aagaatcaat caatttagaa aaagaaataa gaaaaaagat gaatgaaact
    22321 ttagaaaaag caaatggaaa cttaaaaagt cttatgggcg atgaaatgaa aataaaagat
    22381 ctctacaaga aaggacaact agaaaatata agcatagatc aaatcgacct caaagactta
    22441 aaaaaagaac taaacaaact tggagtaagt ttctcagtaa tgaaaaacaa agaaagcaaa
    22501 aactatgaaa tattcttcca agccaaagac ataaaagtaa tggaatatgc ctttaagcaa
    22561 gtcatagcca aggaaaataa aaaagaaaaa gaaagtatcc taaaacaaat aaagaaatac
    22621 aaagacctat ccaaaaacaa agataagaca aaagaaaaag gaaaaaggaa agtaaaagaa
    22681 aaagtaaaac caaacaaaaa agatatgacc agagaaatct aaagggagta acgaaaagtt
    22741 acacccttaa ataaccataa taacaagact tccgaaaaac agaagtccag aattccgaaa
    22801 atcggaaatc aagaatttcg attatcgaaa atcaagaaag gagcagatat ggcaacaaac
    22861 aaaagatact attggataaa attaaaagaa gaatttttca cagacaaaag gata
//
LOCUS       DAVDWT010000033        22026 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVDWT010000033 DAVDWT010000000
VERSION     DAVDWT010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22026
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.033"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..455
                     /locus_tag="VUW50_001601"
     CDS             <1..455
                     /locus_tag="VUW50_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET7983471.1"
                     /translation="KYYVEHPNERPHSDNGFGNASDHVQRNKNGQADTNQTEKPSEEK
                     PQTEKPEEETPREEKPQSEKPESPKPTEEPEESPEESEEPQVETEKVEEKLREAEDLL
                     GKIQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLLALLKESK"
     gene            877..1251
                     /locus_tag="VUW50_001602"
     CDS             877..1251
                     /locus_tag="VUW50_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HET7983472.1"
                     /translation="MESIGLVIVSHSKHIAEGVVELISKVAKDVPITYVRGTEGGGIG
                     TSFEQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNAAA
                     LLQAGAELSVIQTQLAELEINK"
     gene            1709..2422
                     /locus_tag="VUW50_001603"
     CDS             1709..2422
                     /locus_tag="VUW50_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HET7983473.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            2480..3082
                     /locus_tag="VUW50_001604"
     CDS             2480..3082
                     /locus_tag="VUW50_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983474.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRFFSNFDDFLEKNYLKTIWQ
                     VSKETPFSEMDFNMFKNISEKIIFERGSKMLNDLKSNYKK"
     gene            3220..3873
                     /locus_tag="VUW50_001605"
     CDS             3220..3873
                     /locus_tag="VUW50_001605"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET7983475.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            3883..4347
                     /locus_tag="VUW50_001606"
     CDS             3883..4347
                     /locus_tag="VUW50_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET7983476.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            4360..5628
                     /locus_tag="VUW50_001607"
     CDS             4360..5628
                     /locus_tag="VUW50_001607"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET7983477.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIASEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            5642..5902
                     /locus_tag="VUW50_001608"
     CDS             5642..5902
                     /locus_tag="VUW50_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983478.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            5913..7274
                     /gene="pdrM"
                     /locus_tag="VUW50_001609"
     CDS             5913..7274
                     /gene="pdrM"
                     /locus_tag="VUW50_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HET7983479.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSPLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKC"
     gene            7428..8396
                     /locus_tag="VUW50_001610"
     CDS             7428..8396
                     /locus_tag="VUW50_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET7983480.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            8412..9404
                     /locus_tag="VUW50_001611"
     CDS             8412..9404
                     /locus_tag="VUW50_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET7983481.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAVEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            9622..10665
                     /locus_tag="VUW50_001612"
     CDS             9622..10665
                     /locus_tag="VUW50_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HET7983482.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     RVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            10729..12414
                     /gene="lpdA"
                     /locus_tag="VUW50_001613"
     CDS             10729..12414
                     /gene="lpdA"
                     /locus_tag="VUW50_001613"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET7983483.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            12478..13467
                     /locus_tag="VUW50_001614"
     CDS             12478..13467
                     /locus_tag="VUW50_001614"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HET7983484.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANITESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(13540..14610)
                     /gene="xerS"
                     /locus_tag="VUW50_001615"
     CDS             complement(13540..14610)
                     /gene="xerS"
                     /locus_tag="VUW50_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HET7983485.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
     gene            complement(15136..16686)
                     /locus_tag="VUW50_001616"
     CDS             complement(15136..16686)
                     /locus_tag="VUW50_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET7983486.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(16702..17481)
                     /locus_tag="VUW50_001617"
     CDS             complement(16702..17481)
                     /locus_tag="VUW50_001617"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET7983487.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKEN"
     gene            complement(17468..18319)
                     /gene="ylqF"
                     /locus_tag="VUW50_001618"
     CDS             complement(17468..18319)
                     /gene="ylqF"
                     /locus_tag="VUW50_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HET7983488.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQEIQTLAINSKEQVTVKVVTDAAK
                     KLMTDKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(18538..18714)
                     /locus_tag="VUW50_001619"
     CDS             complement(18538..18714)
                     /locus_tag="VUW50_001619"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET7983489.1"
                     /translation="MQAGIPTDNEVSYALIYQQETLPATGSSTSALTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            complement(18766..>22026)
                     /locus_tag="VUW50_001620"
     CDS             complement(18766..>22026)
                     /locus_tag="VUW50_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HET7983490.1"
                     /translation="QNNTKIKQVHVDGVLAGERGIGGLLAKADQSSITESSFKGRIVN
                     TYETTASYNIGGLVGHLTGSKASLTKSKATVVISSNTNSSDQTVGGIAGLVDKDAHIQ
                     NSYSEGDINNSQRFGKVAGIAGNLWDRESNSENHAGRLTNVLSDVNVTNGNAISGYHY
                     NGMKITDAFSNKANKVFNVTLEKDEVVSKESFEERGTMLEASQIASKKAEINLLTPPI
                     VEPLSTSGKKDSDFSKIAHYQANRALVYKNIEKLLPFYNKATIVKYGNLVKENSILYQ
                     KELLSAVMMKDDQVITDIISNKQTANKLLLHYKDHSSEKFDLRYQDDFANLAEYSIGD
                     SGLLYTPNQFLYDQDSIINQVLPELQQVAYDSEAIRKTLGISPEVKQTELYMEDQFTK
                     TKQDLANSLKKLLSADAGLAGDNPVTRGYLVDKIKNNKEALLLGLTYLERWYNFSYGQ
                     VNVKDLVMYHPDFFGKGNTSPLDTLIELGKSGFNNLLAKNNVDTYAISLASHHGTTDL
                     FSTLENYRKVFLPDKTNNNWFKSQTKAYIVEEKSNIEEVKTKQGLVGTKYSIGVYDRI
                     TSATWKYRNMVLPLLTLPEKSVFVISTISSLGFGAYDRYRNSEHKADKDLNDFVEENA
                     RETAKRQRDHYDYWYRILDDNAREKLYRNILLYDAYKFGDDNTVGKATKVASFDDPNP
                     AMKYFFGPVGNKVGHNGHGAYATGDAVYYMGYRMLDKDGAITYTHEMTHNSDQDIYLG
                     GYGRRSGLGPEFFAKGLLQAPDQPSDATITINSILKHSKSDSKEGERLQVLDPTTRFK
                     DATDLQKYVHNMFDVVYMLEYLEGQSIVKKLNVYQKIEALRKIENKYVKDPADGNEVY
                     ATNVVKELTEAEARNLNSFESLIDHNILSAREYQSGDYERNGYYTIKLFAPIYSALSS
                     EKGTPGDLMGRRIAYELLAAKGFKDGMVPYISNQYEEVAKQKGKTINLYAKERGLVTD
                     KLVLDKVFEGKYSSWADFKKAMYDERVKQFGNLKQVTFKDPTKPWPNYATKTINRVSE
                     LQALMDQAVLKDTETPHWYNYNPETDSAVHKLKRAIFKAYLDQTNDFRSSIFENKK"
BASE COUNT         6595 a         4169 c         4555 g         6707 t
ORIGIN      
        1 tcaagtacta tgtcgaacat ccaaacgaac gtccgcattc agataatggt tttggtaacg
       61 ctagcgacca tgttcaaaga aacaaaaatg gtcaagctga taccaatcaa acggaaaaac
      121 caagcgagga gaaacctcag acagaaaaac ctgaggaaga aacccctcga gaagagaaac
      181 cgcaaagcga gaaaccagag tctccaaaac caacagagga accagaagaa tcaccagagg
      241 aatcagaaga acctcaggtc gagactgaaa aggttgaaga aaaactgaga gaggctgaag
      301 atttacttgg aaaaatccag gatccaatta tcaagtccaa tgccaaagag actctcacag
      361 gattaaaaaa taatttacta tttggcaccc aggacaacaa tactattatg gcagaagctg
      421 aaaaactatt ggctttatta aaggagagta agtaaaggta gcagcatttt ctaactccta
      481 aaaacaggat aggagaacgg gaaaacgaaa aatgagagca gaatgtgagt tctagttctc
      541 atttttttca tgaaaatgtg caaaatatag tagattgaaa ctagaatagt atacctctac
      601 ttctaaaaca ttgttagaaa tcgatttgac tgtcctgttc ttatttcatt ttactatatc
      661 ttaacagata gtgtaaataa agataaacta tttactggct aattaatcag ttaaacacta
      721 gttaaggagt aatgatgaaa aaaagaacaa tactattatt gatggccagt ctgttagctc
      781 ttgtcttagg agcatgtggt ttcttggaca tattgatcct ggatcattct catcaggatt
      841 actctttact gctattttag aaactggggt ggtttgatgg aaagtattgg tcttgttatc
      901 gtttcacatt ccaaacacat tgcagaaggt gttgttgaac tgattagtaa agtagctaaa
      961 gatgttccga ttacttatgt aagaggaacc gagggcggag gaattggaac gagttttgaa
     1021 caagtagata gggttgtttc cgaaaatcca gcagatactt tacttgcctt ttttgaccta
     1081 ggttctgcta aaatgaactt aaaaatggtg actgatttca gtgataaaag tatcatcatc
     1141 aacagggttc caattgtaga aggtgcctat aatgcagctg ctcttcttca ggctggtgca
     1201 gaactgtcag ttattcaaac acagttagcg gagcttgaaa tcaataaata aggaatttta
     1261 ctataactct ttttataggt aagctattga ttatctcaac tataaaatgt taagtaaatg
     1321 atttcaaaat cagaaaggga ttgctttatg cagttccttt ttattttaac aggagtgaaa
     1381 ctatagtgtt tctaaattgt gaatcaatca aaactgattg tgatggggct attctagctt
     1441 tagaaacctt caaaaattaa aatttaaggc aatcaattac ttggaagagt atgaaagcat
     1501 ttagtttata ggaattctag gtctagaatt acatatatat ttatgaatac ggggtgttcg
     1561 atagttagta ttgttctatt ctgaaagatt tgagctgtca gttgtataga aagtgttcga
     1621 atttttttaa gtgattaaat tagttaattg tatgaggtgc tttatgatat aatgttctta
     1681 atgaattttc agaaaggaaa acctcaaatt gttctacaaa tttctactct tcgacctcga
     1741 ccacactctt cttgattttg atgctgctga ggatgtggct ttgacccaac ttctaaaaga
     1801 agaaggagtt gcggatattc aggcttataa agattattac gttcctatga acaaggctct
     1861 ctggaaagac ttggagctga agaaaatcag taaacaagag ctggttaaca cgcgcttttc
     1921 tcgtttattt gctcattttg gacaggaaaa agacggtagt tttcttgccc agcgttacca
     1981 attttacctc gcccagcagg gacaaacact atcgggcgct catgatctct tggacagcct
     2041 cattgagcgt gattataact tgtatgctgc gacaaatggc attactgcca ttcagacagg
     2101 acgtttggct caatctggtc tagcacctta tttcaatcaa gtctttatct cagaacagtt
     2161 gcaaactcaa aagccggatg ctctttttta tgaaaagatt ggccagcaaa ttgctggatt
     2221 tagtaaagaa aagacgctga tgattggaga ttctctaacc gccgacattc aaggtggcaa
     2281 taatgcgggg attgacacta tctggtataa tcctcatcac ctcgaaaatc acacacaagc
     2341 ccagccgact tacgaagtct attcttacca agacttgctg gattgtttag ataaaaatat
     2401 tcttgaaaag atcacatttt aaaggagacg agctaatgac tacaaaaaag ctaatattac
     2461 tattgaagag tacattgaaa tgtctgaagt tgattttaat gaagctgtta attatgaatt
     2521 tacatctgac acttgtcaat tagcaaatag tatttatcaa tctcttttta agttttttga
     2581 taagaaaaat ttctctggcg atttaatttt tacttggaaa tctccatcat tagtcaaaga
     2641 aggggattat attgggagaa gggattcaca agtagataat cttagagtaa taggaaatat
     2701 atttccgaat tatcttacta atcgaaaata tagcctcaat atgaatcgta atggctgtat
     2761 gggagatttt cctcatgact tttttgatat atacctagat catgtagcaa aatatgccta
     2821 cgaacaaaaa gttaataata ttaaagagta ttatccttta aaaagagcga ttttacacca
     2881 agagaatgca ttgtattttc gatttttttc taattttgac gactttttag aaaaaaatta
     2941 tttaaagact atatggcaag tttctaaaga aactcctttt tctgaaatgg attttaatat
     3001 gtttaaaaat atttcagaaa agataatctt cgaaagaggt agtaaaatgc ttaatgattt
     3061 aaaaagtaat tataaaaaat agccagtctt tataaactgt attcacgtat tcaaatcgta
     3121 tatatagtcg atttattaaa tctttagata aactgtaaaa agtggtttcc atagccactt
     3181 tttgctataa tagaggcagt aaaaatgaag gagtcggcta tggaacactc gtcttggcat
     3241 gctttgatta aggcgcaatt acctgagggt tatttcggga aaatcaatca gtttatggag
     3301 caggtctatt ctcaggggat tatttatcca cccaaggaaa aggtttttca ggctctcttg
     3361 acaacactgc ttgaagaagt taaggtggta attctagggc aagaccccta tcacggacca
     3421 ggtcaagcgc agggcttgag tttttctgta cctgactcta tcccagctcc accatccttg
     3481 caaaatatct tgaaagaatt gtcagatgat atcggagtta agaaatctca tgatttgaca
     3541 gcttgggctg agcaaggagt cttgcttctt aatgcttgtt tgactgttcc tgctggacag
     3601 gccaatggtc atgctggtca aatctgggag ccttttactg atgctgtgat tcaggtggtc
     3661 aatcatctag atagaccagt cgtttttgta ctctggggag cttatgcacg taagaagaag
     3721 gcattagtta ccaatcctca tcacttgatt atcgaatcag cccatccaag tcctttgtcg
     3781 gtttatagag gattttgggg ttccaagccc ttttccaagg ccaatgcatt cttaaaagag
     3841 acaggacaag agccaatcga ttggcttaga taaggagaaa atatgcctca gttagcgacg
     3901 atttgctaca ttgataatgg gaaagaactg ctcatgctcc atcgtaataa gaaacccaat
     3961 gatgtccatg aagggaaatg gattggtgtg ggtggtaagc tagagagagg agagacgccc
     4021 caggaatgcg cggcgcgtga aatccttgaa gaaacagggc tcaaagccaa gccagttcta
     4081 aaaggtgtca tcacttttcc tgaatttaca ccagatttag actggtacac ctatgttttt
     4141 aaggtgacgg agtttgaggg cgacttgatt gactgcaatg aggggacgct agaatgggtt
     4201 ccctatgatg aggttttgag caagccgact tgggaaggtg accacacctt tgttgagtgg
     4261 cttttagagg ataaaccctt cttttcagcc aagtttgttt atgatgggga taaattgttg
     4321 gatacccaag ttgatttcta tgaataaagg agaaagcaga tgctactaat caaaaatggt
     4381 cgtgtaatgg atcccaagtc tggtttggat caagtttgtg atgtcttagt tcaagatggg
     4441 aaaattatca aaattgcgtc tgagatcacg gaagaaggag cagaaacgat tgatgctact
     4501 ggtcttgtgg ttgctcctgg cttggtcgat attcatgttc atttccgtga acctggtcaa
     4561 acacataaag aagacattca tactggtgcc ctagcagccg ctgcaggtgg ttttactact
     4621 gtcgtcatga tggctaatac cagtccaacc atttcagacg tggagacttt gcaagcagtt
     4681 ctccagtcag ctgccaaaga gaagattaat gtcaagacag ttgcgaccat tactaaaaac
     4741 tttaatggta aaaacttgac tgactttaag gcactcttag aagctggtgc ggttggtttc
     4801 tctgatgacg gtattccgct tgagagcagt aagattgtca aggaagccat ggaggaagcc
     4861 aaaaaactca atacctttat cagccttcat gaggaagatc caggtttgaa cggtgttctt
     4921 ggctttaatg aaaatattgc tagagaacat ttccatatct gcggtgctac tggggtggct
     4981 gagtacgcta tgatggcgcg tgatgtcatg attgcctatg caactaaagc ccatgttcac
     5041 atccagcatt tgtctaagga agaaagtgtt aaagtagtgg agtttgctca ggggttaggt
     5101 gcagaagtca cagcagaagt agcgccacag catttctcta agacagaagc acttctttta
     5161 acacaaggta gcaatgctaa gatgaatcca ccgcttcgtt tggaatcaga ccgtcgtgcc
     5221 gttatcgaag gtctcaaatc aggtgtcatc acagttattg cgactgacca cgcgcctcat
     5281 catgtagatg aaaaaaatgt tgaggatatt accaaagcgc catctggtat gactggctta
     5341 gaaacatccc tgtctctcag cttgacctat ttagtagaag ctggtgagtt gagcttgatg
     5401 gaattacttg aaaaaatgac atacaaccca gccaagcttt acaactttga agcaggttac
     5461 ttggctgaga atggtccagc agatatcact atttttgatg ccaaggctga ccgctttgtg
     5521 gactcccatt ttgcttccaa agcagctaat tcaccattca tcggtgaaac cttaaaaggg
     5581 caggttaaat ataccatctg taagggacaa atcgtctatc aagcttgata ggagttagaa
     5641 tatgaattat tttcgaaaac gtagggagag acaagcgaaa tctaatagtg gaatttactg
     5701 tcccgcagct aatcgttcca aaattcaata tgaaactaaa gaaaaagctg accatgcggt
     5761 tcaatatgat aacggaggtt tagttagaag ctattattgt cgtacatgtg cgtgttggca
     5821 tactacttca aaatctaata aacctccgct aagtttgaaa aactatggtc tgaaactctt
     5881 tggtttagat aaatcagaat agaaagagaa gtatgtataa gacaaagtgt ttacgagaga
     5941 agttagtatt atttttaaaa attttcttcc caatcctgat ctaccaattt gccaattatt
     6001 ctgcctcttt tgttgatact gcaatgacag gtcaatacaa cactatggac ttggctggtg
     6061 tatctatggc aaccagtatc tggaatcctt tctttacatt tctaacaggg attgtgtcag
     6121 ccttggtgcc tatcattggt caccatcttg gtcgaggcaa aaaggaagaa gttgcgtctg
     6181 atttttacca atttatttat ttggccttgg gcctatctgt ggtcttgctg gggatggtac
     6241 ttttcttggc accaataatc ttgaatcata ttgggttaga agcagcagta gcggcagtag
     6301 cggttcgcta tctttggttt ttatctatcg ggattatccc cttgttgctc tttagcgtca
     6361 ttcgttcctt gctggattcg ctgggcttga ccaaactgtc catgtacctc atgcttttgt
     6421 tactccctct caatagcgga tttaactatc tcttgattta cggtgccttt ggtgttccag
     6481 aactgggagg ggctggtgct ggtttaggaa catccttggc ctactgggtc ttgcttggga
     6541 tttctgttct ggttttattt aaacaggaga agctcaaagc cttacacctt gagaaacgaa
     6601 ttccacttaa tatggataaa attaaggaag gagttcgttt aggtctgcct attgggggaa
     6661 ctgtcttcgc ggaagtggct atcttttcag tggttggctt gattatggct aagttttcgc
     6721 ccttgattat agctagtcac cagtcagcta tgaacttttc aagtcttatg tacgcctttc
     6781 ctatgagtat ctcatcggct atggctattg tcgtttccta tgaagtggga gccaagcgat
     6841 ttgatgatgc gaaaacctat attggtctag gaagatggac tgccctcatt tttgcggcct
     6901 tcaccttaac cttcctttac atttttaggg gaaatgtggc cagtctttat ggtaacgacc
     6961 caaaatttat cgatttgaca gtgcgttttt taacttatag tcttttcttc cagttagcag
     7021 atacctttgc ggcgccgctt cagggaattt tgcgggggta taaggataca gttattcctt
     7081 tttaccttgg tttgcttggt tattggggcg tagcaatccc tgtggctacg ctatttgatt
     7141 ccctaacaga ttttggagct tattcttact ggatcggctt gattattagt ttgattgtga
     7201 gcggggcgct ctaccgttgg cgtttaactg tgattatgaa gagatttgaa tctttagcaa
     7261 aatccaaatg ctaaaaagtt tgtgaaaaaa tattcttgac aagaaaatcc cttgttttca
     7321 cggggttttc ttttttattt tgtgaaattt cttttagaaa aactttgaca gtatttgcca
     7381 aaagcggtaa aatagtaagg taagaagaaa gttagaaagg caagaagatg tcaactttag
     7441 ataaaaatct tttgctagaa atgttccgta agatggaaga aatccgtcgc atggacttaa
     7501 aaattgcaca attagtaaag aaagggaaag tgccaggaat gacgcacttt tctgttggtg
     7561 aagaggcagc taacgtgggg gctatgttag ctctcaatcc agatgatctg attacctcaa
     7621 accaccgtgg acacgggcaa gctattgcta aagggattga cctcaacgga atgatggctg
     7681 aaatccttgg taaatacact ggaacctgta aagggaaagg tggatctatg catatcgctg
     7741 accttgatgc tgggaacctt ggtgccaatg gtatcgtagg tggtggtatg gggatcgctg
     7801 tcggtgcagc cctcagtcag caaatgcaaa ataccggtaa aatcgttgtc tgcttctttg
     7861 gagatggtgc gaccaatgaa ggtgttttcc acgaagcagt gaacatggct tctatctgga
     7921 acctgccagt cattttctat tgcattaaca acggttacgg tatctctgcg gatatcaaga
     7981 aaatgaccaa tatagaacat atccatcaac gtagcgccgc ttatggaatt cctggaatgt
     8041 tcatcgaaga cggtaacaat gtcatcgatg tctatgaagg atttcagaaa gctgtggacc
     8101 atgttcgcag tggcaatggt ccagtcttga ttgaaagtgt aacttatcgc tggctcggtc
     8161 actcatcatc tgaccctggt aaatatcgta cgcgtgaaga agtggaattg tggaaacaaa
     8221 aagatccaat cgaaaacctc cgcaattacc ttattgaaaa taacattgca agtgccgaag
     8281 aattggaaga aatccaagcg caagtaaagg aagcagtaga agcttctgtt aaatttgcag
     8341 aggaaagccc attcccaccg cttgaatcag cctttgaaga tatttacgca gactaaggag
     8401 aaagagataa aatggaaaca aaaacaatgt ccttccgtga caccattatc cttgctatgt
     8461 ctgaggaaat gcgtcgcgat gaaaatgtgt tcttgatggg agaagacgtc ggtgtcttcg
     8521 gaggagactt cggtacttct gttggaatgc ttgaagaatt tggtccagaa cgtgtccgtg
     8581 actgtccgat ttctgaagct gccatctctg gagcagcagc aggagcagcc atgacaggac
     8641 ttcgtccaat cgtcgatatg accttcatgg acttctctgt tattgccatg gacaatatcg
     8701 tcaaccaagc tgctaaaaca cgttacatgt ttggtggtaa aggtcaggtt ccaatgactg
     8761 ttcgttgtgc agctggtaac ggagttggct ctgccgccca gcactcacaa tctctagagt
     8821 cttggtttac tcacattcca ggacttaagg ttgtggcacc tggaacacct gcggacatga
     8881 aaggcctgct taagtcttct atccgtgata acaacccagt tattatactt gagtacaagt
     8941 cagaatttaa ccaaaaaggg gaagtgccag ttgatccaga ctacacaatt ccacttgggg
     9001 ttggtgaaat caaacgccaa ggaacggatg taacagttgt tacttatgga aaaatgcttc
     9061 gccgtgtggt tcaagctgtt gaagaattag cagaagaagg aatttcagtt gaaattgttg
     9121 acccacgtac ccttgttccg cttgataagg atatcatcat aaactcagtg aagaagactg
     9181 gtaaggttgt tctggtcaac gatgcccaca aaacaagtgg ctatatcggt gaaatttcag
     9241 ctattatttc agaatcagaa gcatttgact atctagatgc accaattcgc cgttgtgcag
     9301 gagaagatgt gccgatgcct tatgcacaaa acctagaaaa tgcaatgatt ccaacagttg
     9361 aaagtatcaa agatgcaatc cgaaaaactt ataacaaaga atagggttac ataagataag
     9421 ttatgtaaat aaaataaagc tgacgagaaa ctttgtatct tttaggtgca gagctcttcg
     9481 tcattaatat cttagattag agcacgggct aaaagatcgg aaaaaagata aatctccttg
     9541 gcttcatcga agccatcgtc gatttcctat ttttcagtcg cttttgacgc cctttgtatc
     9601 ataaatagaa ttggagaggt catggctgat gacaagctaa gagcgactcc tgcagctaga
     9661 aagttagcag atgatttagg gataaacctc tacgacgttt ctggctcagg tgcaaacggt
     9721 cgtgtccaca aagaagacgt ggaaacttat aaagacacaa acgtggttcg catttcgcca
     9781 cttgcaaaac gaattgccct cgaacataac attgcttggc aggaaatcca aggaaccggt
     9841 catcgtggta aaatcatgaa gaaggatgtt ttggccctgc ttcctgaaaa tatcgaaaac
     9901 gatagcatca aatctcctgc tcagattgaa aaagtggaag aagtccctga taacgtaaca
     9961 ccatatggtg aaatcgagcg tattccaatg acaccaatgc gtaaggttat tgcccaacgc
    10021 atggttgaat cttacttgac tgcgccaacc ttcaccctca actatgaagt tgatatgact
    10081 gaaatgttgg ctcttcgtaa gagggttctt gagccaatca tggaagcaac tgggaagaag
    10141 actactgtaa cagaccttct ttcacttgca gttgttaaga ctcttatgaa acatccatac
    10201 atcaacgctt cattgacaga agatggcaag actattatca ctcacaacta tgtcaatctt
    10261 gccatggcag ttgggatgga taatggattg atgacacctg ttgtttacaa tgctgagaag
    10321 atgagtcttt cagaactggt tgtagccttt aaggatgtta ttggccgtac cttggatggt
    10381 aaattggctc caagtgagtt acaaaattca acattcacaa tcagtaattt gggaatgttt
    10441 ggtgttcagt cctttggtcc gattattaac caacccaact cagctatcct tggtgtcagt
    10501 tcgacaatcg agaagccagt tgtcgtcaat ggtgaaattg tgattcgccc aatcatgagt
    10561 ttaggattaa ccattgacca ccgtgtcgta gatggtatgg ctggtgctaa gtttatgaag
    10621 gacttgaaag agttgattga aaccccaatc tcaatgttga tttaagagca agagtattca
    10681 tttttaagat atagataaag gaaggaagac atggccttag aagtaattat gccaaaagcc
    10741 ggcgtggata tgacagaagg acaaatcgtc caatggaata aaaaagtcgg agaatttgta
    10801 aaagaaggag aaatcctttt ggaaatcatg actgataaag tcagcatgga attggaagcc
    10861 gaagaagatg ggtacttgat tgccattctc aaaggagatg gtgaaactgt ccctgtaacg
    10921 gaagttatcg gttaccttgg tgaagaaagg gaaaacatcc caacagctgg agcagcatcg
    10981 ccagaagcta gccctgtacc tgtagcaagt acctcaaacg atgatggtaa gagcgatgat
    11041 gcttttgata tcgttgtgat tggtggaggt cctgctggtt atgttgcagc cattaaagct
    11101 gcccaactcg gcggtaaggt tgcccttgtt gagaaatctg aacttggtgg aacctgctta
    11161 aaccgtggat gtattccaac caagacctac cttcataacg ctgaaattat tgaaaatatc
    11221 ggtcatgctg caaatcgtgg tatcgtgatt gaaaatccta acttcactgt tgatatggaa
    11281 aaacttttag aaactaaatc taaagttgtt aatactcttg ttggtggtgt tgcaggactt
    11341 cttcgtagct acggagttac tgttcataaa ggaattggta caatcactaa agacaagaac
    11401 gtcttggtaa atggttctga attgcttgaa accaagaaaa tcattcttgc tggtggttca
    11461 aaagtcagca agatcaacgt ccctggtatg gaatctccac ttgtcatgac tagtgatgac
    11521 attcttgaaa tgaacgaagt gccagaaagc cttgttatca tcggtggtgg agttgtcggt
    11581 atcgaactcg gtcaggcctt catgacattt ggttcaaaag tgactgttat cgaaatgatg
    11641 gaccgtatag ttccagctat ggatgtggaa gtttctaaga accttcgctt gatccttgag
    11701 cgtaaaggaa tgaccatctt gactggtact aaactgcaag aaatcattga ggaaaatggt
    11761 caacttcgta tcaaggttga aggaaaagac gatatcatcg caagcaaagc tcttctttca
    11821 attggtcgta tgccagacct tgaaggtatt ggagaggttg agtttgaatt ggatcgtggt
    11881 tgtatcaagg tcaacgaata catggaaact tcagttccag gcatttatgc accaggtgac
    11941 atcaacggta ctaagatgtt ggctcacgca gctttccgca tgggtgaagt ttccgctgaa
    12001 aatgccctta aaggaaatca tgcagttgcc aaattgaatt tgactcctgc agccatctac
    12061 actctccctg aagtagcagc agtaggtttg acagaagaac aagcccgtga gaaatacgat
    12121 gttgccatcg gtaagtttaa ctttgctgct aacggtcgtg ctattgcatc tgacgcagct
    12181 caaggtttcg taaaagttat cgctgataag aaatacggag aaatccttgg tgtacacatt
    12241 attggtcctg cagccgcaga attaataaac gaagcatcaa gcatcatcga aatggaaatc
    12301 actgttgagg aaatgctgaa gaccatccac gggcacccaa cttactctga agtgatgtac
    12361 gaagcgtttg cagatgttct aggaatggcc atccattcac ctaagaaaaa ataaaacgaa
    12421 gatagattag actggaaggt tatttccagt ttctatcata taaagtaaag ggatatcatg
    12481 aaatacatta tcaatcattc aaatgacact gcttttaata ttgccttgga agaatacgcc
    12541 tttaaacacc ttttggatga ggatcaaatc ttcctacttt ggattaacaa gccatctatc
    12601 attgttggtc gtcaccagaa cactatcgaa gaaatcaacc gtgattatgt tcgcgaaaat
    12661 ggtattgagg tagttcgccg tattagtggt ggtggagctg tttaccacga tttaaataac
    12721 ctcaattaca cgattatctc caaagaagat gaaaataagg catttgactt caagagcttc
    12781 tcaactccgg ttatcaatac cctggctcaa cttggggtta aagctgagtt cacaggtcgt
    12841 aatgaccttg agattgatgg taagaagttc tgtggcaatg cccaagccta tatcaacggc
    12901 cgtatcatgc accacggttg cttgctcttt gacgttgatt tgtcagtcct cgcaaatgcc
    12961 cttaaggttt caaaagataa atttgaatca aaaggtgtga aatccgtccg tgcccgtgta
    13021 accaatatta tcaatgaatt accaaaaaaa atcacagtcg aaaaattccg tgatttactc
    13081 ttggaataca tgaaaaaaga gtacccagag atgactgaat acgtcttttc agaagaagaa
    13141 ttggccgaaa tcaatcacat caaggatact aagtttggaa cttgggactg gaactatggt
    13201 aaatcacctg aatttaacgt ccgtcgtgga accaaattca ctagtggtaa ggttgaagtc
    13261 tttgctaaca ttactgaatc aaaaatccaa gacatcaaga tttatggtga cttctttggt
    13321 attgaagacg ttgctgcagt agaagatgtc cttcgtgggg tgaaatacga acgcgaagat
    13381 gttcttaagg cgctaaaaac cattgatatc acacgctact tcgctggtat tagccgtgaa
    13441 gaaatcgctg aagcggtagt tggataaatc aaaaagaagt tggccaacta accaacttct
    13501 ttttttgata tttatttaca taatttaaaa tacgtaaaat cataaactat ccagagcatt
    13561 cttttgttca tcattaacaa tatgggtata gaggtcagtg acttgtgtgc tggcatgtcc
    13621 tagttggtga ctgactaaaa cttgtgattt agtcgcatca tagagcctag ttgctagtgt
    13681 atggcgcagt ttatggggtg ttacacgtac tttaaaatcc tctgagtatt tagcaaccat
    13741 tttctcaacg ctagaagcat cgatacgatt aggaacacct ctgtagagag ttaaaaaaag
    13801 ggctgtatct gttttttccg ttttatagcg ttgattccga atggccagat aattctctaa
    13861 ataaggttta gcaaaagcag cgacattgac tgagtcacgt ttgccacctt ttcgagtaac
    13921 atcaataacc atcattttta gattgagatc tcttagatct agattaacag cttcagataa
    13981 gcgaacacca gatgccaaga gaagggcaat aatggctaaa tctcgttctt tatttttgtt
    14041 gaatgatgag agagctcgat ttgaaagctg ttgtgggtgc tcttgatcga tataagttag
    14101 aaaaccttct gtttcatcac ctagaaagag tttttgcttg atattttcag ctctggcagc
    14161 aagggtttct ttctttttct tggttgaaac ttttttcatt acattacgat agaaataagg
    14221 ttccccctga tcgttttcaa cctcctcggt tagatacttg taaagactag aaagtgctga
    14281 taaggttcga ttgatagttg tctgtgaaac accttgtttt gttgtattag cattcagcaa
    14341 gggacgttca cgtagataaa ggataaagga ttccatgtct ttcttagaca tattttccaa
    14401 aactgataaa ggaatatcgg atattttatc agcgtttgaa ataccagact ccaaaaccca
    14461 gctgaaaaat cggtcatatt ccttaaggta ttcgtacagg gttgtaaaac tgtagggcac
    14521 agccagctta gattggtagt attccagaac ataccagggc atgagttgtt ttagtttgtc
    14581 gattcgttcc agtaaaatct cacgtttcat gtattttctc cataagtaag tatactagta
    14641 gtatagcata gacttgtata tttttcaaga aaattatagt ttttcagaaa gatgttatag
    14701 gagtttttca aagtgataaa aagacttaga cctacaaaca aaacagaata aggaaaataa
    14761 ggaaaataag gaaaataata aaaatctcac ccactaaact tctgttaata ctacatagct
    14821 agaaattggt ttgattgtcc tgattgtttt atcctatttc tatttcattt cactatataa
    14881 ttgctattgt tttctttttc atgattttaa tcttactgta cagatttgat acagtaaact
    14941 cctttctagc tttatacttt atcatttcta acttaattat agtcttattt ttaataaagt
    15001 tcaattattt atatgtaaaa ttcctcatca gtaaaaaata accgatctca tttctgagaa
    15061 tcggttttct aaataaatca agccgaataa atcaagccga tcatttacat aacataaatt
    15121 atgtaaatga tcgtactaca acaacaaatc tttgactttt ccaatttcac tttttggaat
    15181 aaccaggtga atcatatcac ccagatacat tctggttgag ccgttaactg tttggctctt
    15241 gccattatgg acttgagttg tgatgaggac gttgtgtggt aagttgagtt catgaacttg
    15301 tttcccagca attttatcag aaactggtat ttcgataagt gtaacttctc cttcgctaga
    15361 tacttcttct ggaagcattt tttccagcat ggcttcatag actggcgtac ctttgagcaa
    15421 atccatgata atataagaaa caagagtgac aagaccaagt ggcatgaggt tgcgaatatc
    15481 tcctaccatc tcagttacga ggatcatagc agttaaggga gcttttgata tggctccaaa
    15541 atagccactc attcctagaa tgacaaatat agggaattgc tcttgactga caagtccaag
    15601 attgacacag ataacaccaa ctaaggcacc aagcaaagaa ccaagagcta aaatggggag
    15661 gaaaattcct cctggcagtc cacttccata gctaatcata ctccaaataa agcggattaa
    15721 aaagtaagct aataaaactt ggaaactaaa attttgttca gttaaagaaa ggacaagctg
    15781 atttccgcca ccaatgattt gaggtaagaa gattccgact ggtatgataa ggataaaagc
    15841 caagatggga taataagccc tatccaaatg gattttttga ccaatcaagt cataaactct
    15901 tccaacgttt aatacagctt tctcatagag aaaacctgaa aatccaagga aaattcccat
    15961 gacgagatat atccaatact gatctagggt catgggagga atgttatctg gcatatccaa
    16021 tactggtgtc aaaccgaaca tgagtagaga cacaaagttt gctacgatgc tggctgctag
    16081 agttgagacc cagaaaaagc gcgaaaaatg gtgatagact tcttctacaa caaagagaag
    16141 tgctgcaata ggagcattaa aggctgcggc taaacctgct gcagctccac tggcaatcaa
    16201 ggaacgttcc tctactggac tggatttgag ccacttggca attcctttac caccaactgc
    16261 tccaagttga atgctgggtc cctctcgacc cagcatgagt ccactggcaa tagcaagaat
    16321 acctagcaca tattttttcc aaagaatgcc ccaccagttg agggacatga ggcctttcag
    16381 ttcggcttcg acttgaggaa ttcctgagcc tttaatatct ttttctgacc gtgttagttt
    16441 ggcactgagc caacagatga gtatataaaa caaaaccagt acaaaaagat tgcgcactaa
    16501 gtacccttga tcttgataaa ctccttgtat caggtggaag cccttttcaa ttaagaaacg
    16561 aaaggatccg acgataattc caacgatgag gccgacaatg attcctcgac caacttggga
    16621 tagtatagtg ctggatgcaa aggcgaattc tttcttagaa cggactattt ccgactgttc
    16681 ctccatggta tttcctttca attaattttc ttttttacct aaaaccagtg atttaacggg
    16741 ttcaaagctg gttcggtgaa ttggggtaac tcctagtttt gtgaggcctt ccagatgttt
    16801 agctgtgcca tatcctgcat tagtagcgaa atcatagcca gggaactgct gatcgtattc
    16861 cttcatcaat tcatcacgtg ttaccttggc tactatagat gctgctgcga tagagaggga
    16921 gttggcatct cctttgataa tggaggtttg tgaaatgggc aagtccagtt tcatggcatc
    16981 aatcaaaagg tgctctggtt gagggctgag ctgggagatt gcttcttgca tggctagttt
    17041 ggttgcttca tagatgttga cttggtcgat gacctgatta tctatgatac caattccaat
    17101 cgacaaggct tggtcttgaa cggcttggaa aatctccaga tgtttctttt taggaatttt
    17161 cttgctgtcg ttgagacctt taatcttaca atttttaggt aaaataacgg ccgcagcgac
    17221 tacaggacca gcaagaggac cacgaccaac ctcatcaata cctgcaatta aggtcaatcc
    17281 ttgcttataa agttcttttt cataagaaag catggattcc aagcgcaaat tttcatctaa
    17341 ttcagcttga atggctcttt tacgcttgct gatttccttt tgaactcctg agcgattatc
    17401 cttttcaagc tctaaaaaaa tagggctttc taactccttg actgtcacaa ggagttcttt
    17461 gatttcttta atcgttgcca tcgaggtctt ccaatgtatc taaggtatag ttaccgagtt
    17521 tgccatcacg gacttccttc acgaagagac tgtaaaaacg gtcatagtca tcacggaaac
    17581 cgagggcgcg ggtcatatcc ataataatca caggcgcttc ttcttcaatt ttcatttgtt
    17641 tgaagcgttc agccagcttt tctggataat gttctttgaa ataattgata ccaaaaatgg
    17701 taacctcatc cataggaagc aactgatcct tgatagctcc agtcaatgcc aacttaagtg
    17761 caacagtttc atcctcaaac ttaggccaga gaatccccgg tgtatccaag atttccaggt
    17821 ctttattggt tttaagccat tgttgacctt ttgtgacccc tggcttgttt ccaacaacag
    17881 caatcttttt accagccaaa cggttcatca gagttgattt accagcgttt ggaatcccga
    17941 taatcatggt acgcaaggtt tcaatctgaa tcccacgttc tttctggcga gcaatcttat
    18001 cagtcatgag cttcttggcc gcatctgtta caacttttac agtcacttgc tctttggagt
    18061 tgatagctag cgtctggatt tcttgtgatt caaaatactg acgccattcc ttggtcattg
    18121 ctggatcagc caagtcggcc ttgtttaaaa tcaagagttt tggtttatca ccaacaatct
    18181 tggtcaacat aggattttga ctagatagag gtaagcgtgc atcaactaaa atcgtcacaa
    18241 aatcaacaaa ttttaaattc tcctgcacct gtcgacgagc tttagacatg tgaccaggaa
    18301 accattgaat agtagccatt gagttttttc ctttcatagc aaggatttag atcccctatt
    18361 ttattttact attgtttgaa catagagagt tttcaaatgg atgatgtcca aacagttctt
    18421 gttttatgac atagaaatct actttatatt atatcatgcc ttctttattt tgtccgataa
    18481 tagcctttta ccctatcttt taccctatat agaaaaaagc catcaaatga tgacttttca
    18541 gagggagcaa aaaactgttc ccgtcatatt atgaaccaaa agaactaaac taccaacagc
    18601 taataggcct aaagctgtaa gcgcagaagt tgatgaacct gttgcaggaa gagtttcctg
    18661 ctgataaata agagcataac ttacttcatt atcagtagga attcctgctt gaatcttggt
    18721 ttgttcttca tcaccttttt aaactttatt tcctaatagt aaacactatt ttttattctc
    18781 aaaaattgaa cttctaaaat catttgtttg gtcaagatag gctttaaaga ttgctctctt
    18841 caacttatga actgcactgt ccgtttctgg attatagtta taccaatgag gagtttctgt
    18901 atccttgaga acagcttggt ccatgagggc ttgcaattca ctcactcgat tgatggtctt
    18961 ggtcgcatag tttggccatg gttttgtcgg atctttaaag gtcacctgct tcaagttccc
    19021 aaactgcttc acacgttcat catacatagc tttcttgaaa tccgcccaag aagaatactt
    19081 cccttcaaat accttgtcca atacaagttt atcggtcacc aatcctcgtt ctttagcata
    19141 gagattgata gttttacctt tttgtttggc aacttcttcg tactggttgg agatataagg
    19201 taccattcca tccttaaagc ctttggcagc caaaagttcg tacgcaatcc tacgtcccat
    19261 aaggtcccct ggtgtgcctt tctcactact gagagctgaa tagattgggg caaagagttt
    19321 gatggtatag tagccatttc gttcatagtc gccagactgg tactcacgag ctgataagat
    19381 gttgtggtca atcaaacttt caaaactatt caggtttcgg gcctctgctt ctgtcaattc
    19441 tttgactacg ttagtggcat aaacctcatt tccatctgct ggatctttca catacttgtt
    19501 ctcaattttt ctgagagctt ctattttctg atatacattc aatttcttaa caatagattg
    19561 cccttcgaga tattccaaca tgtaaacgac atcaaacata ttgtggacgt atttttgaag
    19621 atctgttgcg tctttaaatc tcgtagttgg atcaagtact tgtaatcgtt ctccttcttt
    19681 actatcagat tttgaatgtt tcaatataga attgatagta atcgttgcat cacttggttg
    19741 gtctggtgct tgtagtaagc ctttagcaaa gaactcaggt ccaagtccac ttcttcttcc
    19801 ataaccacca agataaatat cttgatctga attatgcgtc atctcatgag tatacgtaat
    19861 agctccatcc ttatctagca tgcgataccc catataatat actgcatccc ctgtagcata
    19921 agcaccatgt ccattatgac caactttatt tccaacagga ccgaagaaat atttcatcgc
    19981 aggatttgga tcatcaaaac ttgctacttt tgtagctttc cctacggtat tatcatcgcc
    20041 aaatttataa gcatcgtaaa gcaaaatatt tctataaagt ttttcacgtg cattgtcgtc
    20101 taaaatacga taccaataat cgtagtgatc tcgctgacgt ttggctgttt cacgtgcatt
    20161 ttcttcaaca aaatcattga ggtccttgtc tgctttgtgc tcactattgc ggtaacgatc
    20221 ataagctcca aatcctagac tagagatggt cgagatgaca aagactgatt tctccggcaa
    20281 ggttaggagc ggcaagacca tattgcggta tttccatgtg gcactagtga tacggtcata
    20341 gacaccaata gaatacttag tgccaactag tccttgcttc gttttcacct cttcgatatt
    20401 ggatttttct tcgacaatgt aagccttagt ctgtgattta aaccagttat tattggtttt
    20461 gtctggtaga aagacttttc ggtaattttc caacgtgcta aacaaatctg tcgttccatg
    20521 atggctggca aggctgatag cataagtatc gacattgttt ttagcaagaa gattgttaaa
    20581 gccagattta cctaactcaa tcagagtatc taatggtgaa gtatttcctt taccaaagaa
    20641 gtccggatga tacataacta ggtctttgac attcacctga ccatagctaa agttatacca
    20701 acgttctaaa taagttaaac cgagtagtaa ggcttcctta ttgttcttga ttttatctac
    20761 aagatagcct ctggtaactg ggttgtcacc agcaagtcca gcatctgcag acaagagttt
    20821 tttcaaactg tttgccaaat cttgctttgt tttggtaaac tggtcttcca tatagagctc
    20881 agtttgcttg acctctggag aaattccgag tgtctttctg atagcttctg aatcgtaggc
    20941 aacctgctgt aattcaggta aaacttgatt gatgatagag tcttggtcat acaagaattg
    21001 atttggcgta tagagaagcc cactatctcc aatgctatat tctgccaaat tggcaaaatc
    21061 atcctggtac ctgagatcaa acttttcaga tgaatggtcc ttgtagtgaa gcaagagttt
    21121 gtttgcagtc tgtttgttgg aaataatatc tgtgattact tggtcatcct tcatcataac
    21181 tgcagacaag agttcttttt gatataagat actgttctcc ttaaccaggt ttccgtattt
    21241 cacgatggtc gccttgttat agaaaggtaa caatttttca atgttcttat aaaccaaagc
    21301 acggttggct tgataatggg ctatcttaga aaaatcactg tctttcttac cgcttgttga
    21361 gaggggttcc actattggtg gagtgagaag attgatttct gcttttttgc ttgcaatttg
    21421 agaagcctct agcattgttc ctctttcctc aaaggattct ttgctaacga cctcatcctt
    21481 ctctaaagtg acattgaaaa ctttattcgc tttattacta aatgcatcag taattttcat
    21541 tccattataa tggtaaccac taatggcatt tccattagtt acattgacat cactaagcac
    21601 attagtcaat cttccagcat gattttcact atttgattca cgatcccata agttacctgc
    21661 tataccagca acttttccaa aacgttggct attattaata tccccttcag aatagctatt
    21721 ctgaatatgc gcatctttgt ctacaagacc agcgatacct ccaacggtct ggtcagaact
    21781 atttgtattt gatgaaatga ccactgtcgc ttttgactta gtcagtgaag ctttgcttcc
    21841 tgtcaaatga ccgaccaggc caccgatatt ataggaagca gttgtttcat aggtattgac
    21901 aattcttccc ttgaaactac tctctgtgat gcttgattga tctgccttag ccaacaaacc
    21961 accgatacca cgttcaccgg caagtacacc atccacatga acttgcttaa tttttgtgtt
    22021 attctg
//
LOCUS       DAVDWT010000034        21367 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVDWT010000034 DAVDWT010000000
VERSION     DAVDWT010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21367
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.034"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(299..1147)
                     /gene="dprA"
                     /locus_tag="VUW50_001621"
     CDS             complement(299..1147)
                     /gene="dprA"
                     /locus_tag="VUW50_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HET7983491.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLLTSGQDV
                     LAEFEF"
     gene            complement(1243..1932)
                     /locus_tag="VUW50_001622"
     CDS             complement(1243..1932)
                     /locus_tag="VUW50_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET7983492.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(1944..2822)
                     /locus_tag="VUW50_001623"
     CDS             complement(1944..2822)
                     /locus_tag="VUW50_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7983493.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2812..3681)
                     /gene="licA"
                     /locus_tag="VUW50_001624"
     CDS             complement(2812..3681)
                     /gene="licA"
                     /locus_tag="VUW50_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HET7983494.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3698..4720)
                     /locus_tag="VUW50_001625"
     CDS             complement(3698..4720)
                     /locus_tag="VUW50_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7983495.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4725..5432)
                     /locus_tag="VUW50_001626"
     CDS             complement(4725..5432)
                     /locus_tag="VUW50_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET7983496.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGNRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5768..7255
                     /gene="tacF"
                     /locus_tag="VUW50_001627"
     CDS             5768..7255
                     /gene="tacF"
                     /locus_tag="VUW50_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HET7983497.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFIDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVVPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7265..8068
                     /locus_tag="VUW50_001628"
     CDS             7265..8068
                     /locus_tag="VUW50_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET7983498.1"
                     /translation="MKQLTIEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8070..8879
                     /locus_tag="VUW50_001629"
     CDS             8070..8879
                     /locus_tag="VUW50_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET7983499.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(9154..12330)
                     /gene="carB"
                     /locus_tag="VUW50_001630"
     CDS             complement(9154..12330)
                     /gene="carB"
                     /locus_tag="VUW50_001630"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET7983500.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDTLIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIKSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(12643..13722)
                     /gene="carA"
                     /locus_tag="VUW50_001631"
     CDS             complement(12643..13722)
                     /gene="carA"
                     /locus_tag="VUW50_001631"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET7983501.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            complement(13772..14695)
                     /locus_tag="VUW50_001632"
     CDS             complement(13772..14695)
                     /locus_tag="VUW50_001632"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET7983502.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            complement(14714..15235)
                     /gene="pyrR"
                     /locus_tag="VUW50_001633"
     CDS             complement(14714..15235)
                     /gene="pyrR"
                     /locus_tag="VUW50_001633"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET7983503.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            complement(15446..16075)
                     /gene="nth"
                     /locus_tag="VUW50_001634"
     CDS             complement(15446..16075)
                     /gene="nth"
                     /locus_tag="VUW50_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET7983504.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(16075..16617)
                     /locus_tag="VUW50_001635"
     CDS             complement(16075..16617)
                     /locus_tag="VUW50_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET7983505.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNHLNQEILDDD
                     LVLPIENEALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            16787..17104
                     /locus_tag="VUW50_001636"
     CDS             16787..17104
                     /locus_tag="VUW50_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET7983506.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYEHVKHVLSLYGPV
                     FGF"
     gene            17343..18902
                     /locus_tag="VUW50_001637"
     CDS             17343..18902
                     /locus_tag="VUW50_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET7983507.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQKIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKTAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEICSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(19051..19950)
                     /gene="htpX"
                     /locus_tag="VUW50_001638"
     CDS             complement(19051..19950)
                     /gene="htpX"
                     /locus_tag="VUW50_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET7983508.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALVSAITMLSGMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(19952..20512)
                     /locus_tag="VUW50_001639"
     CDS             complement(19952..20512)
                     /locus_tag="VUW50_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET7983509.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            20606..21319
                     /gene="rsmG"
                     /locus_tag="VUW50_001640"
     CDS             20606..21319
                     /gene="rsmG"
                     /locus_tag="VUW50_001640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET7983510.1"
                     /translation="MKPETFYNLLAEQNLPLSNQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
BASE COUNT         6512 a         4516 c         3949 g         6390 t
ORIGIN      
        1 caaagcgcaa actaggaagc tagccacagg ttgctcaaag cactgctttg aggttgtaga
       61 taagactgac gaactcagct caaaatactg ttttgaggtt gcagatggaa gctgacgtag
      121 tttgaagaga ttttcgaaga gtattatatg agaaaaatcc tctactctac taatccactt
      181 agaaaaaacg catattagca agtgttaaaa acttgctaat atgcgtttta ttatggaaat
      241 gttgggttca ttttctctta aaattgattt tttcttagaa ttctagctta ggtcattttt
      301 aaaattcaaa ttccgcaaga acatcttgcc cactggtgag caattttgct ccttcttgaa
      361 tcaaatgatg gcaaccgtct gatagtccat ctaaaatgct accaggaata gcaaagacat
      421 cgcgtccttc ttccattgct cgctcacacg taatgagact acctgaacgc atcttagcct
      481 ctgctacaat cacaccacga caaagtccag caatgatgcg attacgggca ggaaaatgaa
      541 atttcagagg ttgttcgcca ggtccatatt cacttagaac cagatggtca ttgccgatgt
      601 agtcttgcaa gcgtttattg gctttaggat aaaacacatc cagtcctgtt ccaatcactg
      661 caatggtttt tccgccattc tgaagagctg ccatatgagc tgctgtgtca atgcccttgg
      721 ccagaccact gacaataacc agttcatttt ccaagccttg aatgactttt tcaactgact
      781 tagctccctg tttgctacaa gcacgactgc ccacgaccgc taccttcggg aatttcagga
      841 ggtcaagatt tcccttgtaa aataaaagta caggcgcatc atatatttca ctcaaatccc
      901 aaggataaca gtcatctaaa atagagaaag atggaaattt ttgaaactct ttcgacaaat
      961 gcgcatcgtc tatctgaaaa taacgttcca taaaaacggc tggattacgg caacctgata
     1021 tatctgcaat atcacccaac aaaagctcct gatcaacatt ttcaccgtat tctagcactt
     1081 tcaaaatctg ttgattggtc aaacctgatt tttttaactt atagatttca tagtttgtga
     1141 ttttcataaa taactccatt tctttttcta ctcatctatc tattcgtaaa agttcgtaaa
     1201 aaatcaaaaa acatccgact attttgtcag atgttggaat ctttaatttt cgtttttaag
     1261 aatttcttct aatttacgat agtcttggac actatcgatc tcataaatgc tattgccttc
     1321 taattcttca acatagacat ctagctcttt gatattatcc ttaaccatat tgtcccaata
     1381 gagatcaaca aattcaccac ttgcataagc cttgtcgata aagctgacaa tcttttctgc
     1441 agttggagca tcccagaagg atacaccact aaggatgcga cctgccttgc tatcaacaat
     1501 aatgtcttga accttgtagt catctccata gaccaagaac cattcgttgg tacaatcttc
     1561 acgataaaca ctaaaataag tcgaacgtgt caaatcattg cggaacatat ttttaaagag
     1621 ataattgtca gcatcaataa catagctgtt ggccaattct tcttttacaa gatagagaga
     1681 gtaaaagtta ttgtagtcag cgtatttatc attgaaaacg aggcgaacac cgtatttttc
     1741 ttttaagtaa tcgaattgtt ctttaagata accaacaatg atgatgatgt cattgattcc
     1801 tttttctttg agaaactcaa tttggtattc aatcaaaggt ttttgattaa cctgaaccaa
     1861 ggctttaggg gtattttcag tcataggacg caagcgagtt cccaatcccg ctgctaagat
     1921 aatggctttc acacgaatct cctttattct ttaataataa tataaactcc agcaatgacg
     1981 acaagtgacg tcataatggt cagcatatct agcggtgcac ccaaaaaaac aactgcaaac
     2041 aagaccgtcc atactacata gctcacgttc aagcctgtag ccttggctgg ttgcaagcga
     2101 ttgatagcga tataataagc caagtaggaa atcatatcaa aggctgcaaa aacaatcatg
     2161 agacctagca attgtccatt ggctactgca gtaaatgact gatgagagaa gagcacaatc
     2221 acaagatagg acaagaacga agtcacttga cggattaaga gggcttcgat ttcactcagt
     2281 tcactttcca tagcaaagga gctaagaaca ctctcacttc cccatgcaat agcacaaacc
     2341 aaagcacaga gaatcccaat gtagaaggaa ttaacctgtt caaccttata ggtctgggca
     2401 ataatccctc caataatcaa gacaatccca aatacagtat ttttcgaaat cttgtgcttc
     2461 aaaaagaaga aagccaatag aactgaaatc gcagggtaaa tagccgatac agatgaagct
     2521 aaagaacttc cgatatactt aactgcataa agattggcct gcataccgat agggcctgct
     2581 agcaaggctc cgatgataac actgacattg cgaatattta agaaaattga gaggcgaact
     2641 ttcccttctt ttaccaagag aaaagctagt aagataaaga tgctcaaaaa atcatgagct
     2701 gcagccacca caaagggtga caaatctgta aaaatcgaaa agatataagc actaaccgtt
     2761 agacccaagc cccagaaaat acctgagaga aggccaaaag gaactccgtt tttacttttc
     2821 atctgaacct ccataagaag ccaaaccttt aacagctctt tggtaacgat tcacaccata
     2881 gtcaccaaaa tcttcacctt gctcttcctt atagacagtc catagactcc aaatagtatc
     2941 ttgtaaaatt ttataaatag caatcttttc atgagaaacc ggtgtttggt cactctcata
     3001 gtgagataag aaagtttctt cctcttggga agtgaattca gactctaaaa agagggcagc
     3061 caaatcccac attggatcat tcattgatga atattcccag tcaatcaaat aaagtcgtcc
     3121 ttgaggtgat tcgataaagt tttcaggcac caaatcgata tgacaagatt ttctgtcaac
     3181 acctaagtca gccagtcttt tctctaagga gaagactgca tttctaacag attcatagtt
     3241 ggcataagga atttgttctt caatcaagga ttcgtatttt ttgatttctt caaaaggagc
     3301 aaattctcct cttaattcct tagcagacgt atgaatagtt tgtaatattg gagcaatttt
     3361 gtcgaacttg gtcttgattg acgttgaatc aagcgtaatc gcagattcga tatactcatt
     3421 tactttgata ccagcttcaa tatcaaaaag ataatttttt acatctaagc ctaaatcctt
     3481 tagtagttca agattgtact tttcatcttg tcgattgata agcttttctg tccctttacc
     3541 aaagaattta acaatgtatt gcttatttgt tgttttggcc aaatagtttt gattggtcat
     3601 tccacccagt tgttcaacac tgaggacttc ctcttcttga ctaagtaagg aagaaatttt
     3661 ttctttaatg attttctcca caattaacct ccagtactta tacttcccac ttaaacactg
     3721 ttttaaaggc tgtgtttaaa tcggttgcaa agacacgatg aatatcttta atttctctta
     3781 caggttcttc tagataaagg atatttttaa gacgattggc aaatttcttg acttccatca
     3841 tttggatagc atttttaaaa tcaatgcgac cagaacgaga tgacccaacc aaaatcaagc
     3901 ccttttctaa ggcatcgcga gtattgagat tgactttata ttcgctaact cccatcatga
     3961 gaatcgttcc ctgaggacga atgtagcgaa tcaagtcatt aatagctggt ccagtaccat
     4021 caccaccaca acattcaaaa gcatggtcaa aggccaactc ttcaggaata ttatccgtaa
     4081 tatagcattc tttggcaaat gagaagagtt ccaacttttc ccaatgacga ccaataacca
     4141 caatctctga ttctggcaaa gtatagttga taatattggc aaccacaaaa gctaaacttc
     4201 catctccaat aacggcgatc cgctcccgct tgctatgagc aagagtcaat agacgattca
     4261 tagcgtgcat gcccacactg acaaactctg taatggctgc aaccgtatct tcaatagcat
     4321 cataagccac cacacgatct ttagggagag aaacaaactc tctcataaag ccatcaaatc
     4381 cactagacaa gaaatgggtc cctgtcatgt agttttcata gaattcttca tcactctgca
     4441 taggagactg attgggaatc atgacaactt tttgaccaac ctcgtaggtt ccggtcgggt
     4501 cagaaatgac ggttccacat gactcgtgaa tcattgccat tggaagcttt ttattcaaaa
     4561 tcttgggatc acgttttccc tgatagtaac gctgatccgc atgacagaca gccatgtagt
     4621 tgggacggat aaggatatga ttctcttggt caatagcctc ttcctgatat ttgacattga
     4681 taaacttagg cttagttagt tgataaattt gattaatcat tttactagtc tttctcaatc
     4741 atactttttg caatcttcaa atctgttacg gttgtaatct tcagatttga gtattcacct
     4801 ttggccaagg ccacatcttt tcctttgatc acaaagattt tacatgcatc tgtcaagatt
     4861 tccttctctt catcagaaag agatccataa aggtccatga agtccttgca acggaatgtt
     4921 tgaggtgttt gtccttgata aaggtgagca cgatttggaa tatctgtaat aaattgacca
     4981 ttggtacttt caacgatagt atcaaccgct tctaccactg tgtccactgc gtcatgattt
     5041 tgggcaagtt ggatattgtc ctgaatcatg cgaagtgtaa taaatggacg aacagaatcg
     5101 tgggtaacaa cgatatcctc tggagtaagc ggacgataag catcaatggc ttcaatgatt
     5161 ttcttaatac ttgtattgcg gtcagcacca ccctttgtaa tgatgatacg ttccttataa
     5221 agaggaagat atttatctac aagatcttct gcatgagaaa cccagtctcc atgaacacca
     5281 actacaattt tttcaatact tggctccaag acaaattttt caattgtatg aatcaaaata
     5341 ggtcgattac ctagctctaa aaattgtttt ggcaagttac tgatccccat gcgtgtgcca
     5401 gttccaccgg caagaattcc tgcataaatc atctattttc tcctttgtct tactaaaaaa
     5461 acttattctc tctattatac cacaatctag tcctatcata aaagaaatac tggtcctccc
     5521 cttctagaat aggagaatta tgcagttcta ttattcaaag aaactagtcc atttctagta
     5581 atgactagga attctgtagc aattactaat aaatggctgc gaaattttag agtccttcag
     5641 aataatatac tttccttaaa gaaaacttaa aacttaagat ttgaaacttc aaagtgatta
     5701 cttgacttac tgcccttcat ttcttattag ctaactttat gatataatga aaaacgaggt
     5761 aaattgaatg aaaagtataa aattaaatgc tctatcttac atgggaattc gtgtcttgaa
     5821 tattattttt cccatcctaa ctggaaccta tgtcgcgcgt gtcttggacc gaactgacta
     5881 tggttacttc aactcagtcg acactatttt gtcatttttc ttgccctttg caacttatgg
     5941 tgtctataac tacggtttaa gggctatcag taatgtcaag gataacaaaa aagatcttaa
     6001 cagaaccttt tctagtcttt tttatttgtg catcgcttgt acgattttga ccactgctgt
     6061 ctatatccta gcctatcctc tcttctttat tgataatcca atcgtcaaaa aggtctacct
     6121 tgttatgggg attcaactca ttgcccagat tttttcaatc gaatgggtca atgaagctct
     6181 ggaaaattac agttttctct tttacaaaac tgccttcatc cgtatcctga tgctggtctc
     6241 tattttctta tttgttaaaa atgaacacga tattgttgtc tatacacttg tgatgagttt
     6301 atcgacgctg attaactacc tgattagtta tttttggatt aaaagagaca tcaaacttgt
     6361 taagattcac ctaagtgatt ttaaaccact ctttctccct ctgacagcca tgttagtctt
     6421 tgccaatgcc aatatgctct tcactttttt agatcgcctc ttcctcgtta aaacagggat
     6481 tgatgtcaac gttagttact ataccatagc tcagcgaatt gtgaccgtta tagctggggt
     6541 tgtaacaggt gcaattggag tgagtgtgcc tcgtctcagt tactatctgg ggaaaggaga
     6601 caaagaagcc tatgtttctc tggttaatag aggtagtcga atctttaact tctttatcat
     6661 tccactgagt tttggactca tggttttagg accaaatgcc atcctacttt acggtagtga
     6721 aaaatatatc ggaggcggca tcttgacctc tctcttcgct tttcgtacga ttatcctggc
     6781 cttagatacc attcttggtt cccaaattct ctttaccaat ggctatgaaa aacgtatcac
     6841 agtctataca gtctttgctg ggctactcaa tttgggcttg aatagtctcc tctttttcaa
     6901 ccatatcgtg gttcctgaat actacttact gacaactatg ttatcagaga cttctctact
     6961 tgttttctat attattttca tccatagaaa acaactcatc cacttgggac atatctttag
     7021 ctatactgtt cgatactctc tcttttcact ttcctttgta gcaatttatt tcctgattaa
     7081 tttcgtgtat cctgtggata tggtcattaa tttgccattt ttaattaata ctggtttgat
     7141 tgtcttgcta tcagctatct cttatattag tctacttgtc ttcacaaaag atagcatttt
     7201 ctatgaattt ttaaaccatg tcctagcctt aaaaaataaa ttcaaaaaat cataggagtt
     7261 taaaatgaaa caactaacca ttgaagatgc caaacaaatt gaattagaaa ttttggatta
     7321 tattgatact ctctgtaaaa agcacaatat caactatatt attaactacg gtactctgat
     7381 tggggcggtt cgacatgagg gctttatccc ttgggacgac gatattgatt tatccatgcc
     7441 tagagaagac taccaacgat ttattaacat ttttcaaaag gaaaaaagca agtataagct
     7501 cctatcctta gaaactgata agaactactt taacaacttt atcaagataa ccgacagtac
     7561 gactaaaatt attgatactc gaaatacaaa aacctatgag tctggtatct ttatcgatat
     7621 tttccctata gatcgctttg atgatcctaa ggtcattgat acttgttata aactggaaag
     7681 cttcaaactg ctgtctttca gtaaacataa aaatattgtc tataaggata gccttttaaa
     7741 agattggata cgaacagcct tttggttgct ccttcgaccg gtttctcctc gttattttgc
     7801 aaataaaatc gagaaagaaa ttcaaaaata tagtcgtgaa aatgggcagt atatggcttt
     7861 tattccttca aaatttaagg aaaaggaagt cttcccaagt ggtacctttg ataaaacaat
     7921 cgatttaccc tttgagaatt taagccttcc tgcacctgaa aaatttgata ctattttgac
     7981 acaattttat ggagattata tgaccctacc accagaagaa aaacgcttct acagtcatga
     8041 atttcacgct tataaattgg aggattagga tgcaatattt agaaaaaaaa gaaattaaag
     8101 aaattcaact agccctgctg gactatattg atgagacttg taagaaacat gatattcctt
     8161 attttctcag ttatggaacc atgcttggag ccatccgcca caaaggtatg attccttggg
     8221 atgatgatat tgatatttcc ctttatcgtg aggattatga gcgtttactg aagattattg
     8281 aagaagaaaa tcaccctcgc tacaaggttc tttcctacga tacatcttct tggtacttcc
     8341 ataatttcgc atcgattttg gacacttcta ctgttataga agaccatgtt aagtacaagc
     8401 gtcatgatac cagccttttc atcgatgtct tcccaattga tcgatttaca gacttgagca
     8461 ttgtcgacaa gagctataag tatgtggccc ttcgtcaact agcttatatc aaaaaatcac
     8521 gagcagttca cggtgatagc aaactaaaag attttcttag attatgtagc tggtacgctc
     8581 tccgatttgt caatcctcgc tacttttaca agaaaattga tcaactagtc aaaaatgctg
     8641 taaccaacac tcctcaatat gaaggaggag ttgggatcgg taaggaaggg atgaaagaaa
     8701 tcttcccagt tgataccttt aaagaactga ttttaactga gtttgagggc cgtatgttgc
     8761 ctgttcccaa aaaatatgac caatttttaa cccagatgta tggcgattat atgacaccac
     8821 catcaaaaga aatgcaagag tggtatagtc atagcattaa agcttatcgc aaaaactgat
     8881 tgagggggat tatacaaact actaagatag aggttattca aaaacataat tttagtagaa
     8941 aatgaaatat atattcccac aataaaacgc atcatatcaa ggtttttgaa aaaccttgat
     9001 atgatgcgtt ttataatttt aaagactttt ttctatagta gattgaaata agatgcgaac
     9061 aaatcaatta gaaaattcaa attaatttct agaaatattt tagtatccct gtgtactgtt
     9121 ctaaattcag tctgctatat cttatttttc tatttaaatc gcttctgtaa caaagctacg
     9181 actttcaagt accttaagca tggcattagc tgtatctagc gctgtgaaga ggggcacccc
     9241 gtgttcaatg gctgaacgac gaatttgctc accatcttcg tcagcagttc gttttgttcc
     9301 tactgtgtta atgatagctt gaattcttcc tttgcgtaca aaacttggga tatccttatc
     9361 gtcatcacca atcttaccaa caggttgggc ttgcaagcca tgactagcaa agaaggctgc
     9421 tgtcccttct gtcgcaagga ttccataacc aatgttttgg aaacgacgag ccaagttcaa
     9481 ggcttcttct ttggcatcat cagcgatggt aaagacgaca ttaccaaaag ttggcaagtg
     9541 tagataagaa gcttcaaagg ctttatagag agctttttcc aaagtagcat cagaacccat
     9601 aacttcacct gttgacttca tttcaggacc gagcaagctg tctaccttag ctagtttggt
     9661 aaaggagaag acaggtgcct tgatatgaac acgggtgctt tcagggtaaa gtccattttg
     9721 gtagccaagt tctgataaac tttgaccaag aatgagcttg gtcgctactt gagccatagg
     9781 aatattggtt accttagata ggaatggaac ggtacggctg gcacgtggat tgacctcaat
     9841 aacgtagact ttttcatcct tgataacaaa ctggatgttc atcattccaa ggcagtgaag
     9901 accgattgct aagcgtttgg tgtagtctgc gatggtctcc tgaacctttt gcgacaaggt
     9961 ttgtggtggg taaacagcca ttgagtcacc tgagtggaca ccagcacgtt cgatatgctc
    10021 catgatacca ggaatgagta catttttacc atctgaaatg gcatcaactt cgcactcttg
    10081 cccaacgata taagagtcga caagaactgg atggtctgga ctagccttaa cagcagttcg
    10141 catgtaagaa cgaaggtctt cttcgttttc aacgatttcc atggcacgtc caccaagtac
    10201 ataagatggg cggacaagaa ctgggaagcc aatcttacga gctgcaagag ctgcttcttc
    10261 ttcattggta gccgtttgtc ctggtggctg tggaatatcc aattctttga gagcttgctc
    10321 gaagaggtca cggtcttcgg cacggtctag gtcagcaacc tgtgtaccaa ggatggtcac
    10381 acctgctttt gccaatggct ctgcaaggtt gatggctgtt tgaccaccga actgaacgat
    10441 aactcccttt ggttgttcca agtcaatgac gttcataaca tcttcgaatg tcaatggctc
    10501 aaagtaaagc ttatctgata cagagaagtc tgttgaaacg gtctctgggt ttgagttcat
    10561 gatgatagct tcataaccag ctgcctggat agccttaaca gagtgaacgg ttgcgtagtc
    10621 aaactcaacc ccttgaccga tacggattgg acctgaacct aggacaagta cagattcttt
    10681 atcagatttg atagattcat tttcccaacc ataggttgaa tagaaatatg gcgtttcgga
    10741 atcgaactct gccgcacaag tgtctaccat cttataaact ggaacaatct tgttttccaa
    10801 gcgaagttgg cgaactttat catcagtcgt tccccagagt tcagcaatct tacggtctga
    10861 aaaaccatta agtttggctg ttttcaaaac ttctagatct tgtggatgag cacccaattc
    10921 ttgctcaatt tcaaagatat gcaagagttt atcaagatag aagatatcaa tttttgtaag
    10981 ctctgcaatt tcttcaggtg tgtagccacg acgaatggct tctgatacgt agaagagacg
    11041 gtcatcttgg gctttgacaa ccttttcaat caaggtatca tcagaaactg ctgcaagttc
    11101 aggtatttca ttgtggtgca ccccaatttc aagggagcgg caggccttga gaagagattc
    11161 ctcgatgtta cgaccgattg ccatgacttc tccagtcgcc ttcatttgtg taccgagacg
    11221 gcgttcaccc ttttcaaact tgtcaaatgg gaaacgtgga atcttagcaa ctacgtagtc
    11281 aagggctggt tcaaacatgg cataggttga acctgtaact gggtttataa cctcatccaa
    11341 ggtcaaacct actgcaatct tggcagccaa cttagcaatc ggatatcctg tcgctttaga
    11401 agcaagggct gacgaacgtg atacacgagg gtttacttcg ataacataat acttgaagct
    11461 gttaggatca agagctagct gaacattaca tccaccttca atcttgaggg cacgaataat
    11521 gctcaagctc gcatcacgaa gcatttggtt ttcatagtct gacatggttt gcgcaggggc
    11581 aaatacaatg gaatcccctg tgtgaatccc aactgggtca aagttttcca tgttacaaac
    11641 aaccaaggca ttgtcagctg agtcacgcat cacttcgtat tcaatttcct tgaaaccggc
    11701 aatcgaacgc tcaatcaaac attgggtaac aggtgacaat ttcaaaccat tttcagtgat
    11761 ttcacgcaat tctttctcgt tggcacacat accaccacca gtaccaccaa gggtaaaggc
    11821 tggacgaacg atgactgggt agccaattgt cgctgcaaag gcaactgctt cttctactgt
    11881 gttaacaatt tcagattctg gaatgggttg ttcaagctct tccatcaatt gtttaaagag
    11941 atcacggtcc tccgcttggt caatggcaga taatttggta cccagaagtt caacgccaag
    12001 ctcgtctagg ataccatttt tagataattc catggccatg ttgagacctg tctgaccacc
    12061 gagtgttggt agcaaggcat ctggaccttc cttacgaaga atacgtgtca caaactcaag
    12121 tgtaatcggt tcaatgtaaa ccttgtcagc aatttccttg tccgtcatga tggttgcagg
    12181 atttgagtta accaaaacaa cctcataacc ttcctctttc aacgacaagc aagcctgagt
    12241 cccagcgtag tcaaactcag cagcctgacc aataataatc ggaccagaac caatcaccat
    12301 aattttttga atatcagtac gtttaggcat atataagata ttaagggtgt caagcggaca
    12361 aagctaaaat aggagttatg acgaagaact gtcagttcta ggaataacta tctttttagc
    12421 accgtccgta gcccgtattc agttcagcaa atacggagct cccttctcct ttctattcgt
    12481 cgcctctcag ggcgacatta aataagatac aaaggacgaa tagaaagcga ttgaatttta
    12541 ggaaatcaag gaaggattga caatccaagt tggtttctct acattctgag ctttccgtcc
    12601 gtgttcagtt acataaattc tccgacgagc ttttactcgt tcttagtttg attgtttaaa
    12661 aacttccatc atctcgataa actcgtcaaa taggtagcta gcgtcgtgtg gcccaggagc
    12721 tgcatctggg tggtattgaa cagagaaagc aggttggtat ctgtggcgca caccttccac
    12781 tgacttgtca ttgatttctt cgtgggtaat aatcaagtgc tctggcaaat cctcgcggct
    12841 gactgcataa ccatggttct ggctggtgaa gtctactcgt cctgttgcga tttcacgtac
    12901 cgcatggttg aatccacggt ggccaaactt catcttatag gtcttagccc cgtttgccat
    12961 tgcaaagagt tggtgtccca tacaaatacc aaagattgga atttttcctt gtacaccgcg
    13021 aatcatgtcg agtgcttgtg gaacgtcttc tgggttacct ggaccatttg acaacataac
    13081 tccgtcagga ttgagatgga gaatttcttc agccgttgtc gaataaggaa caactgtcac
    13141 gttacagttg cgtttagaaa gttcacgtag gattgagtgc ttgagaccaa agtccactag
    13201 caccacgctc aaaccaactc ctggagctgg ataagacgtt ttagtagaaa cctgtttgat
    13261 attgtctgtc ggtaaaactg ttgcttggag ctggtccgtc acatggtcca tactgtcccc
    13321 aacatgggtc aaggttgcac gcatagtacc atgcttacgg ataatcttgg taagagcacg
    13381 cgtatcaatt cctgaaatcc ctggaatttt cttggctttc aaaaattcat ccaaggtcat
    13441 ttggttgcgc cagttgctag ctctacgcgc ttcttcaaaa acaacgactc ccttacaagt
    13501 tggaataatg gattcataat catcacgatt aataccataa tttcctacca aaggataagt
    13561 aaaggtcaag atttgtccat tataagactg gtctgtaatg gattcttggt agccggtcat
    13621 ccctgtatta aagacgattt cgcctgttac atcaatatct gctccgaagg ccttgccttc
    13681 aaaaactgtg ccatcttcta atactagaat tctttttgtc atattttcac ctctcgtgga
    13741 cgctcactgg cgtcttttaa cgtcttgtgt tttagttggc gtttctactc gctagtacgg
    13801 attctaagat tgccattcga acaaagacac cattggtcat ttgttggaca atccgtgatt
    13861 ttggtgcttc aaccaagtgg tctgctattt ctacatcacg attgattgga gctgggtgca
    13921 tgaggattgc tgtttctttc aaacgatcgt aacgttcttg agtcaagcca tgttgggcat
    13981 ggtagtcttc ttttgaaaat acagctccac tatcatggcg ttcgtgttgc acacggagaa
    14041 acatcatgac atcaacctga tcaatgattt catcaatggt tacaaactgt ccatagtctg
    14101 caaactcttg acttctccat tcctcaggtc cagcgaaaaa gagttcagat cccaagcgtt
    14161 tcaaaatctg catattggat ttggcaacgc gtgagtggtc caagtcacct gcaatagcaa
    14221 ctttaagacc ctcaaagtgg ccaaattcct cataaatggt catcaaatca agcaagctct
    14281 ggctagggtg ttggcccgaa ccatctccac cattgatgat ggaagtcgta atcgttggac
    14341 tagcaatcaa ttctctatag tagtcgacct ctggatggcg aatcacacag acatccactc
    14401 ctaaagcaga cagagtcaaa atggtgtcat aaagtgtctc acccttatta accgaactag
    14461 tcttcacatc aaagtcaagt cgttccaatc caagtttaat ctctgcgact tcaaaggact
    14521 tatgtgtccg tgtagaatcc tcaaagaaga gattggaaac aatcggatgg tcttcatagg
    14581 gaagctgggc tccattttta aactcaattc ctcgcttgat caatttcatt acttgatcga
    14641 cagtgaggtc ttccatggac accacatggt tcaatgcttg ttgattttct gacatggcta
    14701 ctcctttaac tttctaagct tcttcagtaa tcagaactct gtcttggtca tcaagttctg
    14761 tcatctctac gatgatttct tcagaacgac tggttgggat attttttcca acgtaatctg
    14821 gacggattgg caattctcta tgtccacgat cgactagaac tgctaaactc acacgcgcag
    14881 gacgaccatg accgacaata ttatcaatag cagcacggat ggtacgacct gtatagagca
    14941 catcatccac caagataact tcgcggtctg tcacatcgac agaaaccaaa gaagtatctt
    15001 ctccactttt aacatcatca cggaaaggtt tagtatccaa ttccacaaca ggaactgaaa
    15061 gattttctaa ctgcttcaaa cgttcttgga ttcggtgggc aataaagaca ccacgagttt
    15121 taataccagc caagacgatc ttattcaaat ctttgttgcg ttcgataatc tcataagtaa
    15181 tacgcgtaat cgctcgtttg acggtcaatt cgtctacaac ttcttttgtt ttcatgacaa
    15241 acctccaaaa agaaaagtct ccttaaacaa ggagacttga aatttatagc caagcgagcc
    15301 ctactgcaca cagtatagac ttcacccttc tactttatcg cgctccttgc ctgcctcacg
    15361 ggacaggttt aaaggaatat ttagttatca tttactatag cacaaagcat gcttaaaatc
    15421 aagcaaaaag tttcaatgta gcatcttaca aattgctaaa atcatataat tgtgggtact
    15481 ggtcacactc tggatttttt ggatggcaaa tggctcttcc aaaataaatc atggcctgat
    15541 gggcagctaa ccactgctca ggcggcaaga tatccatgac ccgcttttcc acctcaagtg
    15601 gcgtcgctga ttttttgaca atatcgtggt gtttgcaaat acgctccaca tgagtatcaa
    15661 ctgcaaaggc tggaatccca aatcctacac tcatgacaac attggctgtc ttgcgaccaa
    15721 cacctgccaa actctccaat tcttcacgtg tctgagggac ttgaccatca aaatcgtcta
    15781 gtaactgttg ggcacatttt ttaaggaatt tagctttatt ccgatacaat cccaagcgag
    15841 aaatatgtga agcaatctca ctctctgtcg ctacagacat agcttggggt gttggaaagg
    15901 caacaaagag acctggtgtg gccttattta ccgctgcatc tgtcgtctgg gctgataaca
    15961 tgaccgcaac caggagttca aaatgattgg taaaatcaag actaggcttg gcatctggga
    16021 agagggcaat gatttcttct agcacctttc gtgctcgttt ttttgacaag accattattc
    16081 atctccgtca aatagtcctt gtaagccagc aaaaggactg ttttcttctt tctttactgc
    16141 ttgttgagct tggtattctt cctctgtcat gatttgccag tcatttcctg agacaaatcc
    16201 ttgaccagct tcttcttcag ccgtcaagac cttgatagga atgtttagca agatattgtc
    16261 tgatacactc tcagcaaggt caagcgcctc attttcgatg ggcaagacca agtcatcatc
    16321 taaaatttct tgatttagat ggttagttgc gccttccatg aaaacttccg tgactggata
    16381 agattcaact aactcaactg gctccatact gcgactcgaa gcaagaacaa tggtataaga
    16441 tagttgataa tctaagaaat acatacggtc ttcatattgt actttcccaa ctgcaaggat
    16501 atcttttaca tctaaaattt cttgattacg tgcacgcagg tcatcaacta aatctaacgt
    16561 ttgttcaaag ttcaaacctt cagactgctt acgaatttct tgaatattta atttcatact
    16621 tcctccataa agatttactc tcttgattat accatgaaaa ggctacaaat cagcacacca
    16681 aactttgtaa ttaaaattca gaattttaac atatttacta tgacagtttt gttttttagt
    16741 gctatactat agggaaagag tacatcagat caaggaggat gctcacatgg aagacaagaa
    16801 actcattcaa ctcctatcca agttaaataa aagctaccaa aactgtaaac agggtacggc
    16861 agatgatatt cgactacaag agctgctaaa cactactatg caagagctca aaaaaacgga
    16921 aaagttgaac aacagtatct taattgatct tgagaaattt taccaaccca ccagtcttct
    16981 gattggactg ggtagcctaa aactaaacga tcaagcacgc actgcttggc gaaactatga
    17041 taaattccat tacgagcatg tcaaacacgt actaagtctc tatggacctg tttttggatt
    17101 ttagagcata gaatttccag ttttctgttg acaaaatttc cttaaaggta taatataaag
    17161 atactaatac tcggaggtaa gggagacatg aacaactaag tctatcaaat aaagaacctt
    17221 tatttagtag atcttgtttt tgtctctttt tgtgtgctct tttatgctct ttttctggca
    17281 tgttaataga gtttttttga catagacttt gggctctact aggtaaagta gagctttttg
    17341 ttatgcacta tgaacattct agaaagggaa atcatatgat aaaaatcaat catctaacca
    17401 tcacacaaaa caaagattta cgagatcttg tatctgacct aagcatgacc atccaagacg
    17461 gggaaaaggt tgctattatt ggtgaagaag gaaatggcaa atcaacctta cttaaaattt
    17521 taatggggga agctttgtct gatttcacta tcaagggaaa catccaatct gactatcagt
    17581 cactggccta cattcctcaa aaacttcccg aggagctgaa aaagaaaact ttacacgact
    17641 acttcttttt agattctatt gatttagact acagtatcct ctatcgtttg gcggaggaat
    17701 tgcattttga tagcaatcgt ttcgcaagtg accaaaagat tggcaatcta tcagggggag
    17761 aagctttgaa aattcagctt atccatgagt tagccaaacc ctttgagatt ctatttttag
    17821 atgaaccttc aaatgaccta gaccttgaga cagttgattg gctaaaaggc cagattcaaa
    17881 agaccaggca aaccgttatt ttcatttccc atgatgaaga ctttctttct gaaacagcag
    17941 acactattgt tcacttgcga ctggtcaaac accgtaaaga agcggaaacg ctagtagagc
    18001 atttagacta tgatagctat agtgagcaga gaaaggctaa ttttgccaaa caaagtcagc
    18061 aagctgctaa caaccaaaga gcctacgata aaaccatgga aaaacatcgg agagttaagc
    18121 aaaatgtaga aactgcgctt cgagctacca aagatagtac tgccggtcgc ctattggcta
    18181 aaaagatgaa aactgtcctc tcacaagaaa aacgctacga aaagacagct cagtccatga
    18241 ctcaaaagcc acttgaagag gaacaaatcc aacttttctt ttcagacatc caaccattac
    18301 cagcttctaa agtcttagtc caactggaaa aagaaaattt gtccattgac gaccgagttt
    18361 tggttcaaaa actacaacta actgtccgtg ggcaagaaaa aatcggtatt atcgggccaa
    18421 atggtgttgg gaaatcaact ctgttagcca aattaaagca acttctgaat gataaaagag
    18481 agatttccct tggttttatg ccacaagatt accacaaaaa actgcaattg gatttatcac
    18541 caatagctta tatcagcaaa actggggaaa aagaggaact tcagaaaatc caatctcacc
    18601 tagccagtct caatttcagt tatccagaaa tgcagcatca aattcgctcc ttatctggcg
    18661 gacaacaggg aaaactcctg cttttggatt tagtcctgcg caaaccaaac tttctcctgc
    18721 tggatgaacc cacacgaaac ttttctccca cttctcaacc ccaaatcaga aaactctttg
    18781 caacctatcc aggcggtctc atcactgttt cgcatgaccg tcgtttctta aaagaaatct
    18841 gctcgatcat ctatcgcatg acagaacacg gtttgaagct agttaattta gaagatttat
    18901 aaatttgcaa catagcagat aggtggggca aaaacacaat tttaggagaa aaagaagtaa
    18961 atcttcccat agtaaaacgc ataatatcaa gttttcacac ctgatattat gcgtttttca
    19021 gattttaaag acttttttcc agccttcatt ttacatctgt tttaaacgtt caatccgttc
    19081 tgagataggt gggtgggtat aaaagagttt ttggaacccc ccacctttct tgggatcatt
    19141 gatataaaga gcactgctag catcatcgac gtggcgactc ataggtttgc tattgtccaa
    19201 cttatctagg gcattaatca ttccctgggg attgcgagtc agctcgacac tagatgcatc
    19261 tgccagaaat tccctctgac gagaaatagc gagctgaacc aaggttgcag cgagaggtgc
    19321 cagtacaata gctagtaggg aaaccactag cataatgatt tcaagaccat ttccatctcg
    19381 gtcatcatca cttcgtctgc gacctgctcc accccaccac atcatacgac ctgccatacc
    19441 agaaagcatg gtgatagcac taacaagggc aactgcaata gtcgaaatac ggatatcata
    19501 attacgaata tgactgactt catgtcccat aacagcttct agttcttcac gattcatgat
    19561 agctagtaga cctgaagtcg cagcaacagc cgcattttga ggattagaac ctgtcgcaaa
    19621 ggcatttaag gctggatcat caatgatgaa aacacggggc ataggaatct gagcgaccag
    19681 agccatatct tccactacat ggtagaggtc tggtgccgtt tgctcatcca cctcacgcgc
    19741 tccattcatg gacatgacaa tctctgtcga ttgaaaaatc atagacaaag cgtagataaa
    19801 gccgataatc agtgcgataa ccaaaccacc aagtccagat cttataaaga gataaccaac
    19861 cgcataacca acaagagcta agagtaggaa aaataccagc aacaaaatcc aggtttttcg
    19921 tttattgctt gcaatttgat caaacaacat cttagtcacc taaaccgcta aaatcaactt
    19981 taggaaccga cttttcctct tcaggtgttt gaaggaaatc tgccgcttta aatccaaaca
    20041 ttccagcgat aatattgctc gggaaagttt ctaattttac attgtagttg ctgacaacac
    20101 tgttatagag ttgacgagag taagaaattt tattttctgt gtttgtcaac tcctcttgca
    20161 atttaacaaa gttagcacta gctttcaaat ctggatagct ttctgcaact gcaaaaatac
    20221 ctgaaacctg acgagtgagg gcatcactgg ctttcatagc ttctgctggt gaagtcgctg
    20281 ccgccacttg gttacgtagt tctgccacct tttcaagggt agaaccttca tatttggcat
    20341 aaccttttac agtctcaatc aagtttggca agagatcatt gcgacgtttc aactgaacat
    20401 caatctgact ccaagcctcc ttggtttgca tacgattttt aaccaaaccg ttatagctaa
    20461 caatcacaaa aataacaata agagcgataa ctccaagaat aatccaagtc atgatataag
    20521 tcctttctac ttttagatta gtaccagtat atcaaatttt taatgattgt ggtaaaataa
    20581 gatgatacta aagaaggaaa taactatgaa accagaaaca ttttacaact tgcttgccga
    20641 gcagaatctt ccactttcga accagcaaaa agaacaattt gaacgttatt ttgagctctt
    20701 ggtcgagtgg aatgagaaga ttaatttgac ggcgattacg gacaaggaag aagtttatct
    20761 caaacatttt tacgattcga ttgcacccat tcttcaaggt ttgattccca atgaaactat
    20821 caaacttctt gatatcgggg ctggggcagg atttcctagt ctaccaatga aaattctcta
    20881 tccggagtta gatgtgacca ttattgattc actcaataag cgcatcaact tcctacaact
    20941 cttggctcaa gaactggatt tgaacggagt tcatttctac cacggacgtg ccgaagattt
    21001 tgcccaagac aagaacttcc gtgctcaata tgattttgta acagctcgtg cggttgcccg
    21061 tatgcaggtc ctatctgaat tgactattcc ctaccttaag gttggtggaa aactattggc
    21121 actcaaggcc agcaatgcgc ctgaggagtt gttagaagct aagaatgccc tcaatctcct
    21181 ttttagtaaa gtcgaagaca atctcagcta cgccctacca aatagagatc cgcgttacat
    21241 cacagtggta gaaaagaaaa aagaaacacc aaataaatat ccacgtaagg ctggtatgcc
    21301 aaataaacgc ccgctttaaa tttttaagta aaaaaatatt tacaaaatca acctcgctct
    21361 cttattt
//
LOCUS       DAVDWT010000035        20907 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVDWT010000035 DAVDWT010000000
VERSION     DAVDWT010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20907
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.035"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            1..276
                     /gene="tnpB"
                     /locus_tag="VUW50_001641"
                     /pseudo
     CDS             1..276
                     /gene="tnpB"
                     /locus_tag="VUW50_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(1000..2727)
                     /gene="ezrA"
                     /locus_tag="VUW50_001642"
     CDS             complement(1000..2727)
                     /gene="ezrA"
                     /locus_tag="VUW50_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HET7983511.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLESGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2809..4755)
                     /gene="gyrB"
                     /locus_tag="VUW50_001643"
     CDS             complement(2809..4755)
                     /gene="gyrB"
                     /locus_tag="VUW50_001643"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET7983512.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFSSHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4770..5333)
                     /locus_tag="VUW50_001644"
     CDS             complement(4770..5333)
                     /locus_tag="VUW50_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7983513.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(5418..5972)
                     /locus_tag="VUW50_001645"
     CDS             complement(5418..5972)
                     /locus_tag="VUW50_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET7983514.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYQDVFGKNQ"
     gene            complement(5983..7206)
                     /locus_tag="VUW50_001646"
     CDS             complement(5983..7206)
                     /locus_tag="VUW50_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET7983515.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(7243..9693)
                     /locus_tag="VUW50_001647"
     CDS             complement(7243..9693)
                     /locus_tag="VUW50_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HET7983516.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSCRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKSGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9893..10822)
                     /locus_tag="VUW50_001648"
     CDS             complement(9893..10822)
                     /locus_tag="VUW50_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET7983517.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQAFLLDEEVHKLHPKNRFLDTGFYDYDPDMVR"
     gene            10962..11249
                     /locus_tag="VUW50_001649"
     CDS             10962..11249
                     /locus_tag="VUW50_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HET7983518.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRK"
     gene            complement(11283..12617)
                     /gene="ciaH"
                     /locus_tag="VUW50_001650"
     CDS             complement(11283..12617)
                     /gene="ciaH"
                     /locus_tag="VUW50_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HET7983519.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12607..13281)
                     /gene="ciaR"
                     /locus_tag="VUW50_001651"
     CDS             complement(12607..13281)
                     /gene="ciaR"
                     /locus_tag="VUW50_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HET7983520.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(13390..15936)
                     /locus_tag="VUW50_001652"
     CDS             complement(13390..15936)
                     /locus_tag="VUW50_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET7983521.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLALRFDQAAGELALSNMP
                     EIDVDNRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(16059..16499)
                     /locus_tag="VUW50_001653"
     CDS             complement(16059..16499)
                     /locus_tag="VUW50_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HET7983522.1"
                     /translation="MTPQEMWNKYKQINPSIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCIVEITKVSVKPFNRVSADHAYKEGE
                     GNKTLVYWRQVHEDFFRDCLGEAGLTFTPESKVVLEEFRKVYPL"
     gene            complement(16474..17121)
                     /locus_tag="VUW50_001654"
     CDS             complement(16474..17121)
                     /locus_tag="VUW50_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HET7983523.1"
                     /translation="MKNQLALSGEKILEKIYPLFGYRGTRRLLACLDEFQAEAHVVTE
                     VYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIELPSAYFDLSSILETGI
                     SKIVVGMNDLTSFVFATMRNSQWHDLESPIMLDMLRDMQDKARKNKINFAVAGYLNTS
                     FIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKKLQRSTHDTARNVE"
     gene            complement(17099..17599)
                     /locus_tag="VUW50_001655"
     CDS             complement(17099..17599)
                     /locus_tag="VUW50_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7983524.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(17610..18308)
                     /locus_tag="VUW50_001656"
     CDS             complement(17610..18308)
                     /locus_tag="VUW50_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HET7983525.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     RFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18301..18567)
                     /locus_tag="VUW50_001657"
     CDS             complement(18301..18567)
                     /locus_tag="VUW50_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HET7983526.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(18633..19559)
                     /locus_tag="VUW50_001658"
     CDS             complement(18633..19559)
                     /locus_tag="VUW50_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HET7983527.1"
                     /translation="MRYITLGQDEKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19634..20449)
                     /locus_tag="VUW50_001659"
     CDS             complement(19634..20449)
                     /locus_tag="VUW50_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983528.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLMPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPSAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20453..20773)
                     /locus_tag="VUW50_001660"
     CDS             complement(20453..20773)
                     /locus_tag="VUW50_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET7983529.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         6283 a         4553 c         3730 g         6341 t
ORIGIN      
        1 aagacagata tgaggtaggg aatagactca ttagcctatg tagttaaaac ccactttgaa
       61 ttggattctt tctccggtca agtctttctc ttttgtggtg gacgtaaaga ccgctttaaa
      121 gccctttact gggatggtca aggattttgg ctactatata aacgctttga gaacggaaaa
      181 ctgacttggc taagtacaga aaaggatgtc aaagctctcg cacctgaaca agtagattgg
      241 ctgatgaagg gcttttctat cactccaaaa atatagtaga ttgaaactag aatagtacac
      301 ctctgcttct aaaatattgt tagaaatcga tttgactgtc ctgatcgatt tgtcctgttc
      361 ttgtttcgtt ttactataga acgatttgaa ggcgtttatg atatttagct gtacgagagt
      421 cttttaaaag tgttttgatg gtttggattt cttctttaat tgatttcata ttactattgt
      481 ataatgcttt ttgattttag tctagtataa atagcgagaa atatctatcc tatcttctag
      541 aatatcttcc aaacgagaaa actctcgtaa acaaagaggt tttagaggcc tatttaccgt
      601 ggactaaagt tgtacaagaa aagtgcaaat aagaaatctc cagattagga actatccgtg
      661 agttctctag tctggagatt tttcaataga cttcgttatt ggacggttac caagttactc
      721 aaagtacaga tttgaagctg cagataagac tgacgaaatc actttaatac taaatgaaaa
      781 tcaaaaagca aactaagagc ctcaggctgt acttgagtac ggtaaggcga cgctgacgtg
      841 atttgaagag attttcgaag agtataaaac actgttttga ggtggtggat aaatcggctg
      901 ggcaaaaact caatctcagg agaaaatgaa gtaaatctcc ccacaactat agaaaaagat
      961 ttgattctgc tcctcacaca ataaaatctt tttcttttat taaaaacgaa tcgtttcacg
     1021 tgttttctca tatgaggtaa caaagcgatt ggttacacca ggctctgcca cttccaatgc
     1081 ttgagaaatc ttgtcaaatg aagcgtgata atcaaattct ttttcaaaaa tatctaaagc
     1141 ttcgttaaat gcttcttgaa tgcgttcatc aaatgagcga tagcggttag aatattgtaa
     1201 gagttgctct gtcaaagttg catattgtac aatattataa gtttccgttt ctaaagcttc
     1261 catatcattc gttgcaattt caagaactcg ggtaacagat tcaatgttaa tcattttttg
     1321 ttctaactca accattaaat cctcggtatt attgcttgcc gtaaagaata acttcaagaa
     1381 agtttgtgga atacctggca gattgcgttt ttccatgtat cgcttgatag tatggagacg
     1441 attgacataa acattggcct tttgacgtgc attaatatca tctttctcaa tttgtgtcag
     1501 gcgctcacta actgaaattt gctcatcttc aatatctttt agttgagttt gtaaatcctc
     1561 aagattttct tcaagaactg aataagcttg ggttggttct tcttgatttg aagttacctc
     1621 aacaatagct gcctcaaaac tctctaattc tgtctgaata cgacgaacat ggcttgcagc
     1681 tgtctcagga agtaaatagg tcttgttcaa acgtgcaata tcttctccca ataaagtatt
     1741 attctctttc atatgttgaa gataagttgg aagagttgca agtagatttt ccactacttt
     1801 ctgagcagca atttctcgag taaaaatatc atacaaggca ttgatttcct cttgtgcctg
     1861 tccattctca tattcggcat tatccaattc caactgacga atattctctt ggtttttctt
     1921 gaatgcttca taaagcaagt ggaaacgcgc ttcaatatcc gtttcaacaa aatgataatt
     1981 agcatcaatt agtttacgat aaccggattc caaatcctgt aattgatctg gcaattctgt
     2041 agaaagcgtc gtaaccaagg ctggaacacg atccacaata tgacttaagg ccaaaatgtg
     2101 attttctgta ttatccaaaa tcactgcggc ttccacaggg tcacccgatg aattcaaggt
     2161 tacaaattgt gaaaattcag attggatatt ttctaattgt ttttcaattt catccaaggc
     2221 ttgaccatac tgttctgaat tttcagcaac tctatgctga agttcctcaa ataaatccaa
     2281 agcatgaaga acacgaccac tatttttaga ttcttgcttc tctaagtctg ccaaagcatt
     2341 gcgaattgcc gcaatatctt cttcaatcaa agtaatttga ctctcaattt ggtcaatttg
     2401 atgactggcc ttgagaaaac gaaatgaatg gttatagcct tctgcttcaa agagattatt
     2461 ttcaatatcg gcaaaagagt tgagagataa atcgacccat ttttgattcc attcacggaa
     2521 agccacttga ctttgtccaa tcaagtgcat attttttaca gcttctactt catcatttac
     2581 tggaagattg tatagttctt cttttctttc ttctagcgcc tctaatctcc cctcgtttcg
     2641 cttacgtaga aagattgcca ctacataagc cagaactaaa atgactgcaa ttgcaaccat
     2701 taaataaatt agttgtccat tagacatatc aaactccttt tttacttgaa acaatcgtaa
     2761 cgattatatc atatttttta gaccaaggga actacttctc cctatttttt agacatcaag
     2821 tgtactatag acagcatttt cttcgataaa ctcacgacga ggctctactc gatcccccat
     2881 caacatatca aagattttat ctgcttctgc agcatcatct acagaaactc tagccatcaa
     2941 gcgatgttcg ggatccatgg ttgtttccca cagctgatga tcgtccattt cacctagccc
     3001 cttataacgc tgaatagtcg gtttggtacg accttcacta taacgggcta aagcttcttg
     3061 gagtttgatt tcttgatctg cacccggctg gatatattct ttaatctcgc ttccaacctt
     3121 gacaccatag attggtggtt gggcaatata aacataacca gcttctagga ttggtttcat
     3181 ataacgataa atcaaggtta aaagaagggt acgaatgtgg gctccatcga catcggcatc
     3241 ggtcatcaaa acgagttttt ggtaacgggc tttcgaaaca tcaaattctg cgccaaatcc
     3301 tgttcccatg gctgtgaaaa gactacgaat ttcttcgtta gctagaatct tatccatact
     3361 tgctttttca acgttcaaaa tcttaccgcg aattggaagg atagcctgaa actcacggtt
     3421 acgaccagat ttggctgatc caccagctga gtctccttcg acgatgaaga gttctgtttc
     3481 agcagggtta ttagaagaac agtctgctag tttccctgga aggttggaaa tttccaaacc
     3541 agatttttta cgtgtgactt cacgcgcacg cttggcagcc acacgagcct tggcagctaa
     3601 aatccctttt tctacgatac gtttggcaat ctgtggattt tccatgagga aatcggagaa
     3661 ggcttcactg aagaggcgat tggtaatctt gaccacttcg ctatttccca atttggtctt
     3721 ggtttgtcct tcaaactgtg gatttgggtg tttaactgag ataactgcag ttaagccttc
     3781 gcgaacatct tcccctgtta gattgtcttc attgtctttc agtaacttat ttttacgagc
     3841 ataatcgttg ataacacgtg tcaaggctgt acggaaacct tgttcatgcg ttccaccttc
     3901 atgtgtatga atattattgg cgaaactcat gacattttca tggtaacccg ttgtgtactg
     3961 catggctacc tcaactgtga tatcatccat ctcaccgtct gtatagattg gtgtatcaaa
     4021 gattacatcc ttgttctcgt tgatatattc aacgtaacta gcaatcccac cttcataatg
     4081 ataatgcttg gtttgttcca aaccttggcg cttatcagtg atagaaattt gaagaccgcg
     4141 atttaaaaag gccaactctt gaatccgttt atttaattta tcaaaatcaa aggttgttgt
     4201 ttcagtaaag atttttgggt ccggtatgaa gtgaaccgtt gttccagttc tatctgtatc
     4261 cccaaccacc tcaaggtctg caacaacatg accacgacgg tattcttggt aatgaatctt
     4321 accgttttta tggacatgaa cgtctaattg agtggaaagg gcattaacta ctgacgaccc
     4381 caccccgtga agaccacctg aaaccttgta tccaccaccg ccgaactttc ctccagcatg
     4441 aaggactgta aagacggtct caacagcagg acggcctgtt ttttcctgaa tatcgactgg
     4501 gataccacgc ccatcatcca caacagtaat cgaatcatct ggctcaataa aaacttgaat
     4561 atggctggaa aatcctgcca aggcctcgtc aattgagtta tcaacaattt cccagactag
     4621 atggtgaaga ccttcttttg aggttgatcc aatgtacatc cctggacgca tacgaacagc
     4681 ctctaagccc tctaaaactt gaatttgact ggcatcataa tcctgtgcct gcagattttt
     4741 gatttcttct gtcatcttat tcctttttct tatatgtcta atacttgtct taaattcacg
     4801 atactggtaa acaagtgggt atcaagtcct gcagcttgac ctgcttcgat atcaatcggc
     4861 cgatcaccaa tgacaagacc agagctaatc tgatactttt ctcttaaata aagcatggat
     4921 tcgggatttg gctttctctt aaagcctgag ctagaagtca ccacttctgt aaaataagct
     4981 gctatagagg ttttttctaa aatttccaaa acctgatcat ttcgatgaga gaccaaaaaa
     5041 tgacggccac cttgatttga aatgtcttcc aataggtcag aaactccttc aaataaaatc
     5101 gggtgttcaa gctctctggc ttcattttcc ttgtactttt ctaaaaaatt ctctaaattg
     5161 ggagcgaatg tctcaatcgc aaaaggagta gaaaccttta aagcttgata gacactgtca
     5221 tggtcttgtg tgataccata cagtgccaat gtttcaacaa atgcagctgt tgaagtttca
     5281 taattatcca gtaaagttcc acctaaatcc cagatgtaat cgtgatattt catacccttc
     5341 attatagcat ttttttatga aaaaagcgat gatttccctg agttttccac ataaaaaacg
     5401 agagaataac tcccgtttta ctgattttta ccaaagacat cttgatagag cattcctgtt
     5461 cgtaaactct ctgcgtcccc tcctagttgc tccaccaact cgtaggcaaa ggcaagggct
     5521 gttgaaggac cccgactggt tgtcaactga ccatctacca ctactgtttc cttgacgtag
     5581 tgaccatcaa ggatttgctc ttgaacgccg tcataacaag tgtatcgctt atttttcaat
     5641 atctctgctt gattgagggc aattggtgcc gcacaaatgg ctgctagttt cttcccttct
     5701 tgctcgaagc tttgcaattc ttgaatcaag gtctgattat cacgtaaatg tgcagaacca
     5761 ggcatacctc caggaagaac aatcatatca tagtctgata aatctccatc aaagacatga
     5821 tctgctctta cttggattgc atgcgaaccc gttacttgct cttcaaaacc aaccatatca
     5881 catgtaatat tggctcgacg caagacatct acaactgtca aggcttcaat ttcttcaaag
     5941 ccctgagcta acataactgc tacttttacc atgattttct ccttatttaa tttgttttaa
     6001 tacaaccttt ttccgtttga atgggacttt tccgctcttt tcttcagcta aattagtctg
     6061 gtaactcatc gataaaagca aaccaacacc aatcaaatta ctgataatag ctgatccccc
     6121 ttgcgaaatg aaaggcaagg gaatccccgt caaaggaagt agtccagtca cagcaccgat
     6181 attctcaaag atgtggaaga gcaacatcat aatcaaacct gtggaaatat aagtgtagaa
     6241 ctggttattt gatttaagag taatcttcaa catacggtaa atcaacatga gatagagggc
     6301 aataaccagg acagagccaa taaagccaaa atcttctgca ataaccgtaa aaatcatatc
     6361 tgactctcga actgggataa gcagattcga agcattaaat ccctgaccaa ataagccacc
     6421 actcccaatg gcaatctgcc cttgagcctg ctggtaagtc gttgtttggg caaactcaaa
     6481 gggattgagc caagccaaaa tccgattaat ttggtaggtc ggcattccaa tctggtgaag
     6541 aaaagctcgt ccgtccttgc taataaagat agctaagaaa ccagcaactc ctgttacagc
     6601 agtcacaaat actgggataa taattttcca agaaacccct gataataaaa cgattcctga
     6661 gaaaatggct acaaaaacca aagccgtccc caagtcactt tgaagtgcta aaagaactag
     6721 gactggaatg gtaaagagaa tcatccagaa aattaacaaa aagtccagcg gaaccgtgcg
     6781 tctccattcc ttatgtttct ttgtaaattg gacaatgaca cgagccaaca tgaggatata
     6841 ggatatcttc ataaattctg acggttggaa tagggtaatt ccatttattg atacccagtt
     6901 tttggcaccc gttgatgcaa ctaagcttgg attataaaat acaatcggca agatcataag
     6961 tcccaagcct aaaatatata gaaagggggt caccttccaa agaaattctg tattaaagag
     7021 catgaccaca aaaccaatca caagccccaa ggcaatccag gcgacctgct gccctaaaat
     7081 gggcagaata ttattgggat aatcatgact aacggctata tagatagcca ccacaccgat
     7141 gaccagtaga aaaaatactg gcaagagcaa actataatcg actctagagt cgagagaacg
     7201 tttcatatat actaacctta tactttcata caatactatt tatcaaagtt cattaaaaaa
     7261 tctatcaata gcctcgtcaa cttcggatcg agagatggtt ttaacagtcg cttcttctgc
     7321 tagagatgct actatttgtt tgccgtatcg ttttccgacg attctcctat ccaaaataag
     7381 agttaaggaa cgttggtatt cacgtctcat acttcttccc aaagcctgtt ttaaacgaat
     7441 aatggccatt ggtaattgat aatcataaaa ggcatttttc ccttcttgat tcagttcttg
     7501 attaatcttt ttcgtcaagg gttcttgagg attttggaaa ggaagcctcg gtacaacttg
     7561 aatcacaaaa ggatggcttg aaaaatcaac tccctcccag aaacttgctg caccaagcaa
     7621 gatttgttgt tcaccttttt caaagcgttt ctttagctga tgaacatccc cacttttata
     7681 ctgggccaag tggctaactg taagtaaatc cgatactgct agaagcatgt ctttagcggt
     7741 aaagagaacc aaaatcggtt gctggaaagc ttgaatttcc actagtaaag cagctacctc
     7801 tctggcatag acttctaagg aggtttctgt taccagaggg aaatctttga ccatgaccac
     7861 ttcttgttcc tgttttcccc gagattcaat cttgacaaat ttggcttcag gatagcctaa
     7921 aaggtctgcc aaagaaaccc tctgactaat ctcaagagta gccgatactc ccaagacttg
     7981 gcaactctca ggaagtaaag aggacagaag agtacggcat gattttgtag aagaaatctg
     8041 aattttcttt tgactcgttt cagttacttc aagccagtaa tcaccttcag ctgtaaaata
     8101 gcgaaccagc tcatcaaagc cttctacttc taattctgaa aaatactgat ggagattgtc
     8161 cagagaatct aagatatttt tcctagattt accagactga aattgttcta tcaagtagag
     8221 acactcaaag cgaatacttt ctagtatccg ttgttgaacc ctgttttctt ctcctactaa
     8281 agccttatca attaaatcga taatagattg tatatcgtag gtctcttgaa gcagattttc
     8341 tagagctaac aaaatctttt ggacttcatc aataatcagt aaacggtcac tgacaaattc
     8401 aggattatct tcaagtctgg tcacgagata ggcatgatta gtcactaaaa gcttgcaagt
     8461 ctctgccctt tcttgactac gtttccaaaa atcttccgtc acaaataagc tctgggatga
     8521 taaattccca tcatgacgaa ggtctgctag aaaatgttgg taacggtaga gttgcccgat
     8581 ttcatccaaa tctcctgtct ctgtctcagt aagccagacc aagacttgca ttttaaagcg
     8641 tctaaataag cgattttcat cattttcctg caaggaatga taaaaggcat ccaacttcag
     8701 ataattttgt ggtcccttta agctatgaat atctgtatgg aacacttcct tgaggcgttt
     8761 accttcttct tccatgattt gattttgaag aatctttgtc ggaacactaa gaacaatttg
     8821 tcgctctttg gattgagata aagcgggtaa gagatagcca taggttttcc caatccctgt
     8881 cggcgcttga atcagagaga caggttcatc tttcaatagc aagccaacct ctttagcaaa
     8941 actttcttgt tcctccctca cttcaaggtt caacagagaa atatttttag aaaagtcttg
     9001 agatagtttt cgtagctcca gagaagctgc cgttttctta aaatacagac cttgaacttg
     9061 gaccaagtct ggagaactca ggatagattg gttgcgataa gtttcctcaa taaccaggta
     9121 ggactcatat aggagagcgt cagccatttc cagcaagcgt tccaagagac ctttaggaag
     9181 ctgggtcatc ttttcccgta aaaaaagaag taattctgct gtagcttggg catctgaaag
     9241 ggctgtgtgt gcgtgtttaa gaggaattcc taattctcga cacaaaatcg gcaagctata
     9301 tttttccagt tcagggaaaa agacctgggc caattcgacc gtatcaacac gagggtttct
     9361 tagctcataa ccttcaaaaa ataaattttc cgccaagaga ttagcatcaa actgaacatt
     9421 atgggctaca aaaatcccat cctccaccaa gtcaaagatt tttctggcaa cttgcgaaaa
     9481 atcaggtgct tgcgccagac gttggtctgt caatcctgtc agttctttga tatgagcatc
     9541 caagggttca tgtggattga catccgtcgt atagtgatcg acgatttctc catcctcaat
     9601 cacgacaatt cccacttgga taattttagc cttactacct gtgctagttg cctctaaatc
     9661 caccacaaca tagcgattac caatcgtttt cattataaaa aattatacca aaaaaagggc
     9721 catttggcac ctgcaaacat aggataattt tcaaactcaa gaacactact tttcttaaaa
     9781 atcccatcga atagagcatt ctagcctgct gatttttgta aaatagtgaa gaaatacgaa
     9841 aaattgagag aagaaatgtt tgcatcttcc ttctctcaac gaatcatgta ttttatctaa
     9901 ccatgtcagg atcgtagtca taaaatcctg tatcaaggaa acggttttta gggtgaagtt
     9961 tatgaacttc ttcatccagc aagaaggctt gatcatggct aaagttgccc tcttgaatca
    10021 aattacgcgc ttggataaag agttttgcaa tctcaacaaa gttctgacca ggagtgtaga
    10081 gcccttctag tttccagcga gtaaagccat gctccaccaa ttctgttaat ttgatcatca
    10141 aatcaaggtc attgttggca aagatatggg tcccatgatt atcttcaaaa atggaatagt
    10201 ggctctctgg atcacttggc tcagccaaga agaggtcacg tttatgcgtc ttttcatcat
    10261 cgatatgtgt aaagttatag tagttttgca agagtggacg tttagaatga tggatgacgc
    10321 tagcaccgta aaccaaaact tcagcaggaa tttccaaaat ctctggcatt ttgaaaagtt
    10381 cagctgatgg aatttcacgc gccaaaacag cctcagatgc gccagccttt tgtccccaga
    10441 agttaatctg acgactgcta gttaccatgg ttgaagcatc gtagatggtc ttaaatgaat
    10501 aaccatcgcg gttaactacg taaaagacgc ctgcatcccc aatcgtaata tagtctgtct
    10561 tgatttcttc caagaagtct aagaaaggct tgatacggtc catcatatct tggtgcatga
    10621 gagcattgac cgcaacgatc aattccttac cagcatcatg aaccaactta gcgatttcac
    10681 gtaattggtc ataactaaag gtcgttggca gacgaaggcc aaaatctttc tcaccgacat
    10741 agatacggtc tacgccagct tcgagtagtt gttcaacttg ttcaatactt tcagcagttg
    10801 ctgtaatgac aatcttttcc ataagaaaaa ttataccaca tttctccaaa atcagccatg
    10861 ctttgaaaat gaaaagggac tttattcttt ttgtaacctt tctgtaataa ttctctgccg
    10921 aaaaaatgat aaaataggta tgtactgtta aggagagaat catgcccgta agaaaattac
    10981 aatcctatga ggtagactat caagaagaat taaaccagca gcttcctcat taccaagctt
    11041 atacacctga agcgcaatct gatgccaatc tcaaggaaat cctatttttt attaatatcg
    11101 ctgttttttg tatctgtatt gctatcttta gttttatctt tttagcatta aaattatcaa
    11161 ctgctcttgc ctttgccgca gcaatcggat tcagcttact tgttttaaaa gttcaacggt
    11221 ctattatcaa acgaaaacgt agaaaataaa tccaaaatat cgccccaatt ggagcgatat
    11281 ttttattttt tctttttaga tggtgtctga atggcaatct tcacttcaaa gattgttccc
    11341 ttgggtttat tatctttgac agtaacagtt ccttttagag catctacaat ttgcttggct
    11401 agggataatc ctaaaccaaa accacctttt tgccgggttc tagccttgtc tactcgataa
    11461 aaacggtcaa aaattttctt tttatcttct gtcgaaatac cgattccatt atcagaaaca
    11521 agtaaataaa gattgcgatc ggtcgccgag ataagaaaat caatttcacc atcctcctca
    11581 gtatacttga cggcattatc gaaaagaatg gtcatcagtt gtttcagaag aagctgatct
    11641 gtgacaattg ttcgatggat acgattttca aaacggaaga cacgattatt ttccgaagca
    11701 atcatctcat agtttgtgaa agttgtatta aaaaagctag ttggaacttc tgcaagctcc
    11761 ggcttaatcc catcatctct ccgagctaag ttcagcaagc ttgtcgttaa aaaacgcata
    11821 tttcggactt cttccaaact cgatgcaatg ctttcgctca catccataat ggtagcttct
    11881 ggcttacgaa aaagggtctc taagcgattt tgcaaaactg cgagtggagt tcgtaactca
    11941 tgactggcat tttccacaaa agactgttgc ttctgcatac tctcaagcag gggcctaaca
    12001 ctgaccctag ctagatagag actggcaagt aaagacaaaa tccagaaact agccatcacg
    12061 accacaatca attgctcatg cttttgacta gcctgctcca actgactggt attaatcaag
    12121 acagcagcat acttgatatt ggttgaaacc gaactaatat tggtctccat caaaatcaca
    12181 cgatagattt cttcctgccc atagctatta aaaacctgaa tctggtagat atgtcctagt
    12241 tctttctttt ctaacttaat cttatccaag cccaaaaatc gatttccaga aagaagttga
    12301 gtaaagtcct tatcaaagag aataacttcc gtattggaac tgacattagg ttttatttct
    12361 actttactag cgtccgccct agcattttct aaatctttaa tctcttctgt tgccctattt
    12421 atagccagct gaataactgc ttgaggattt tcactcagtc catgaagctt atcgtccacc
    12481 gaagtataaa gactcgaatg catgacttgt aaaataatca gagtcattgt agaaaaaatc
    12541 agggtgaaga caccgaagtt gcggataaaa taactaaagt catccgcata ccatgttttt
    12601 ttaagtttac tgaacatctt ttaaaagata cccaacacta cgcaaggttt gcaaattctc
    12661 tgcaaaagtg gttcccttta atttcttacg gacttttgaa acatagactt cgacaaccga
    12721 aatcgttgta tcactatcaa atccccatag acggtcaaaa atctgcgtct taggcaaaat
    12781 cacattttga ttttgaagga aataaactag taaatcgaac tctttcccca gcaattcgac
    12841 aggagtatct tcaactttaa cggtattggt tgataaatta accacgatat tcccataagt
    12901 caaggtgttt tcattaaact tccctgaacg tttgagaagg gcctgaatcc gcattttaag
    12961 ttcttctagg tagaaaggtt tggtcagata atcatccgct cccagttcaa atccatgtcc
    13021 cttgtcatcc aaactttcct tggcagtcat aatcagaact ggtgtcgtaa ttcccttttc
    13081 acgcaattct tttaagactt ggaaaccatt tttttctggc aacatcaaat ccagcaaaat
    13141 caaatcatag acaccactct cagcttcgta gagaccttct tctccatcaa atacctgcat
    13201 aacatccgca aaatcgtcta aaaagtcaaa tactgaattt gacagaccta ggtcatcctc
    13261 aaccaataag atttttatca tgagaaactc ctccttatta aaactattat accaaatttg
    13321 ccttaaaaaa aactcaactc tctgcatttt acatgagata gctgagtttt ctttttattt
    13381 taggcttatt tatgcatttc cgtattgaag aacaactgct tcgactgcag ctttttcacg
    13441 gctaatcaag tcaacacgtg ctgcaatttc cttgattccc ataccgatgt tacggctaag
    13501 agcaaggtca gaaagttgcg gctcaaagaa ctccttgtat tccgccaagc gttgctgagt
    13561 cttaaataca tgagcaggaa ggataacaaa gctatcaaag ctcatatctc ctccaagggc
    13621 tgccttaatc caagcccagt tttcacgcgc ccaagaccaa gctgttttct gagttgcttg
    13681 atgagctagg aattggtaat accaagcaga caagtcctgt ggtttgacca caaatttgtc
    13741 cttccaagaa gtaatcaagt tttggatatt atccgcatct gtactgtatg caagagctgc
    13801 tgccaactgg cgtttaaaga cagcatctgt tgcgtgagta taagtatcaa gataaagtgc
    13861 taacaagtct ttagtctcat gatgtttcat ctcattaatc agaacttgtg agcgaatagc
    13921 tgctgggagt cctgcaagat tctccttgtg tgttgcgaag atttggctag cgacttgact
    13981 agcttctgca tcatttgagc gaatcatcat cgaaacagcc agctgacgaa ccaattcatc
    14041 ctcatctgat tctccgtctt tagcttcaaa accaagacgg tcatagttat gacgagccaa
    14101 tttagcaacc agtcctttga aggctgtttc agcatccgtt ccttcatcaa taaagcgctc
    14161 aagggctgaa atcacttgag aaacagctga aaccaccaga taagactctt ccttagcaag
    14221 tttatcaaga actggaagca agtctgcata agaaatgtgc cctgcctcag ccaacaaacg
    14281 acgttcttga acaatttgca gtttgcttgt gttatcaagt gtctctagct cagcaagaac
    14341 agcttctaac aagtctcctt gatagtcggt aatatagtgg gcagtatttt cagtgttgag
    14401 acgaagagct ccttcattct cagcaagaag agctgcgtag ccagggattt cgatactttc
    14461 agtttcgagt gtatcaggca agcctttcca gttgctattg aggggcacca cccagagacg
    14521 gttcttgtct tcgttctcac cgatgaagaa ttgtttttgt gaaatcttca agacatcatt
    14581 ttcaactttg acagtaagaa ctgggtaacc aggctgttcc aaccaagaat ccatgaaggc
    14641 tgcgacatca cgtcctgacg cttgaccaag ggcatcccaa aggtcactac caatggtgtt
    14701 gctgtattgg tgtttttcaa agtaggcgtg caaaccttta gcaaaatcag catctcctag
    14761 ccaacggcga agcatgtgca tgagacggct tcctttggca tagacgatag cgccgtcaaa
    14821 gagtgtatta atttcatctg gatgtttaac ttcgacgtgg acagactgaa cgccatcagt
    14881 agcgtcacgt tcaagagcaa gaggtactcc acctgtttgg aaatcttcaa agatattcca
    14941 gcttggttcg atggtatcca cacagacgta ttccatcata ttagcgaaac tttcattgag
    15001 ccaaaggtca tcccaccatt tcatagtcac gaggttccca aaccattggt gagccaattc
    15061 atgggccaca acaagggcaa cttgttgacg gctagcaaat gtagagttct catcgacaac
    15121 caagtaaact tcacggtagg tcacaagacc ccagttttcc atagcaccag ctgagaagtc
    15181 aggaagggcg atgtggagag attgagggat tgggtattta acaccgtagt aatcttcgta
    15241 aaactcgata gagcgaacag cgatatccag tgagaaatca agatttgaaa gtggatgtgc
    15301 tttggttgag tagacaccta ccagggtacc gtttttagtt ttagccgtca caccttgcaa
    15361 atcaccagct acaaaggcca acaagtaaga tgacatacga ggtgttgtct caaacttcca
    15421 aatacctgtt tccttgcggt tatccacatc aatctcaggc atgtttgaca aggccaactc
    15481 acctgctgct tggtcaaagc gaagagcgag atcaaaggtt gctttggctt ctggctcatc
    15541 cacacatggg aaagcttcgc gcgcaaaatg gctctcgaac tgagtagaca agacctcctt
    15601 cttgactcca tcaactgtat aataagaagg gtaaatccct gtcatgttgt ctgtaatttt
    15661 accagaaaag gcaagaacca attcaacttg accagcctca gccaattcga tatgaagggc
    15721 ttcattgtca tggtcaactg taaatggacg agcttgacct gcaacttcta cagaggtgat
    15781 ttccaagtct ttttggtgga gggagatgcg gtcactctgt gcttgaccag tgatggtcac
    15841 tttcccagaa aaagtcttgg tctcacgact caaatctaaa aataaatcat aatgttcagg
    15901 aacaaattgc ttaataaaat gttcaactgc ttgcatagtt ttctcctatt ctaagtttaa
    15961 gagctgctgc tcttcttcaa acaaacttat tatatcatat tttgcttgag aaaaaaggat
    16021 tggacagcga tttccaaaac tttcttcctt ctaatagtct acagtgggta gaccttgcga
    16081 aattcttcta aaacaacctt gctttcaggt gtaaaagtca atcctgcttc acccaagcag
    16141 tctctgaaaa aatcctcatg aacctggcgc caatagacaa gtgtcttgtt tccctcacct
    16201 tccttatagg catggtcagc tgacactcga ttgaagggtt taacagaaac ctttgtaatt
    16261 tcgacaatgc agacagcctg attttgacta tctaaaatga catcgaaggt ccctacttgg
    16321 ggaagtggtt cgtcttctag tacatagagg tcataggctg atgctgttgc tgtcttttct
    16381 cctttaaaca ccaaatccgc taaaaggtct ggttcaactc caaaagccca ggcatcgatt
    16441 tcatctccga tagaaggatt gatttgtttg tatttattcc acatttcttg cggtgtcatg
    16501 ggtgctcctt tgtaattttt tactttcttc ttttatgtgt ttaagatgat ctggatggtc
    16561 aatctctaaa tcaaaaatct ctggaataga actgtagtgg ataatgcact tgatacccaa
    16621 ctgattcatt ttttgtatga aagaagtatt cagatagcct gctacagcaa aattaatctt
    16681 gttctttctt gctttatcct gcatatctct tagcatatct aacattattg gactttccag
    16741 atcatgccat tgactgtttc tcatagtcgc aaaaacaaag gaagtcaaat cattcattcc
    16801 aactacaatc tttgaaatgc ccgtttccag tatactagat aagtcaaaat acgctgacgg
    16861 taattcaatc atcgttccga ctttcccagt aaagccatac tgacgcaata ctgtaatagc
    16921 ttgttttaat tggtcggcat cattgacaaa aggaaagata agagatagat tggggttggt
    16981 ttgataaact tctgtaacga catgtgcttc agcctgaaat tcatccaaac acgccagtaa
    17041 acgcctggtt cctctatagc caaacagagg ataaattttt tcaagaattt tttcgccact
    17101 aagggcaagt tgatttttca tcattcacct tccaattcat gtaagaagtc ttgtccagtt
    17161 ctggaaatcc taataattca gacttaacct tcaagactaa tggcgatgca ttttcttctg
    17221 taatctcttg aatatccatc caaatatatc caagtgaatc attcgcacca tcagacacag
    17281 cttccgaagt cgtaacttga ggtgcactct cattcatttc aacatcatac aaggccatga
    17341 catggtgaac cataaaattt gttaactctt ctctgacgaa aacatcgtag attcgaggat
    17401 tagagtagct tctaacagta aatcccgtct cttccataac ttctctagtc agcgtttccg
    17461 tcagtccttc accaagttgc tgactgcctc caggtagatc ataccgatgt tgataagggc
    17521 ctctcgtttt ttcaatgcag agtaacttcc cattttctaa gcaaacagcg tagactccaa
    17581 agtgtttttt gatttccatc caactcctcc tacttcaaag accagccacc atctattgtc
    17641 aagatttgtc cttgcatggc gctcgctttt ccacttgcta aaaaaagact aagctctgct
    17701 atttcctctg gctcaatcca gcgcttgatt ggagtttcac tagccaccca gtcagccaaa
    17761 ccacctggtt caaaatccgc agcggtcata gctgtcttga ctgctcctgg agcgatacca
    17821 aagacctgaa tcccagcttc agcatagtct agagccaact gcttggtgaa gccagccaag
    17881 gcatgcttgg atgaagtata ggcgtgacca cctccacctg ctaggctaga agcaatggaa
    17941 cacatattga tgatgattcc ctttttattt tccagcattt gtgtcaaata ataccgagtc
    18001 aactctactg gaataatgta gttgatttca aaaatctctt gaatgtcctg cgccgtttgt
    18061 tccaacagtg gtttgtaatc atccaaaact ccagcagtat tacacaaaac atccacctga
    18121 gggcaccagt caaaaatagg ttccaagtcc aaggtcaaat ctctctgtaa aaagcgaaaa
    18181 tcaccctcta agagtggctt ttcaccttgg tcaactccat aaacttgata gcccttctct
    18241 aaaaagaggc gagcttgagc caatccgatc cctgaactca ctcctgtaat gagtacacgt
    18301 ttagtcatgc acttctaccc aatccgttgc caaaacatca caaactgtcg ggctccacat
    18361 ggaaaaacct tctccttcgc cagaaacgtt gattaggaaa taaggtgtca tttcaagtgc
    18421 aagcccattt tgctcgatgg tatcaaagag ttggacatag ttttccgcac ctccccaacc
    18481 agttcgtaca tattttctct tagcctttaa cccaggcagg atctcttcaa atgtcatgtt
    18541 tttctccttt aattctacat tcttcattta attatagcaa aaaaccgctt tatacggctt
    18601 tttgaatgtg agttattcaa acctgctact acttacggca aattattccc tgcagcaaga
    18661 taaatttcat accattcttt tcttgttaag ctaaagtttg ccgctcggct aacttctctc
    18721 aagtgcttag gatttgttgt acctacgact gcctgcattt ttgctggata acgcaatatc
    18781 caagaaatgg caatagttga agaggttact ccatatttaa tagctaaacg atcaagtact
    18841 tgatttaaag cttgaaattt ctcatttcca acaaaattcc ctttaaaata cccgaattgt
    18901 aagacagacc atgcttgaat gaccacatcg tgtaattggc aatattcaaa aatgctgcca
    18961 tctcgcatag ctgcttgact atcttccata ttaacatgaa aagctgattc aaatcctgga
    19021 gtaaaagccg cactcaattg tagctgatta acagctaacg gctgcttgac atctttttta
    19081 agcaactcca tcatcatagg attttgatta gaaactccaa aatctcgaac tttaccttgt
    19141 ttataaagga gattaaaggc ttctgctact tggtcagatt ccatcaaagc atctggtcga
    19201 tgaaggagca agctatctag atgatcaatc ttcaatcttt gcaaaatacc gtctactgat
    19261 tttataatat agtccttaga aaaatcaaaa taggtaaatt cttcaatgcg aatgccacat
    19321 ttggactgaa tccacatctt ttctcttaaa tctggacgat tttttaggac aagacctaac
    19381 agttcttcac aacgaccacg accataaata tcagccaagt cgaaggcatt gattccaaca
    19441 gaaagtgctg tttctacaag ctcttcaact tcttttacag atttatcttt tattctcatc
    19501 attccgagaa caatttctga taattctttt tcatcttgac caagagttat gtatctcatc
    19561 aaatttttct cctttaattt ctaacattct tcccttcatt ataacaaaaa accgctttgc
    19621 aacggctttt tgactatact tcactccatt ttatcttctt aaacccacgg aacaagacaa
    19681 agattccaat aaagaggaca gctgaaggaa taacttttgt aaggaaaaca tttgaaattc
    19741 ccatccactc ataataacgg agcagagaac ccaccacaag atgggcaata atcatactga
    19801 caaatggacg aaagaccgct tctttccaat tccaaatacc gataactagc gaaatcgtaa
    19861 agacagacaa actatcccag ggagccggaa tataaaaggc tccttcttgt atgaagcttg
    19921 ccattcctac atatcctaaa acaactagaa gaactatagt cccaacaaca atgtaagtgc
    19981 caattttcat tttaggagaa tcttggacta aacttcttcg taaaattgtg gccacaagtc
    20041 caaatccaat cagaaaaata agaagttgcc ctaaaaatgt gagcaaattg actgttaaga
    20101 gaggaccttt agaaaaatca cttagtagtt gataataacg taataccgcc aggacaagaa
    20161 ttggcatcaa aagggactct ttgatagaac tgcgaggtgc tcccttgaga atctctttca
    20221 ttattttttt aggattctta cctagataat cctctgcact catgccatct cgttctgctt
    20281 ctgagaaatc tagcatcatc aaatagatct gctctctgag atagtcttca tcatagagaa
    20341 atccagcaag attaaaactt tcccacaact cctcaaaata cttttgattc tcctcagaaa
    20401 actcatgtag caaagcgctt gtttcttcgt aatacttcat tttcttcatg gtttaacccc
    20461 cattcttaat cccttctact ttttgactca aatcgtccca ttgttgccaa aagactgaga
    20521 cacgctcttc tccttctttc attaatgaaa aatacttcct atctggacca tctggcgacg
    20581 ggcgcatgtc gcctcttatc cattgatttt tttctaactt ttgcaacaaa ggataaatag
    20641 ttcctggaac gatagtatca aatccagcct ctcgcaaagt ctgaaccaac tcataaccat
    20701 accgctcttt ttgaccaatc atatccaaga cacaaccttc aagaacacct tttaatagct
    20761 gagtttcttt catcacttct cccttctaat ctattttgta atacctacta gtaacttcac
    20821 ctatagtata tcacttctac actagtttgt aaagcataat agttaatact cttcgaaaat
    20881 ctcttcaaac cacgtcagcg tcgccct
//
LOCUS       DAVDWT010000036        18610 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVDWT010000036 DAVDWT010000000
VERSION     DAVDWT010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18610
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.036"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..674
                     /locus_tag="VUW50_001661"
     CDS             <1..674
                     /locus_tag="VUW50_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HET7983530.1"
                     /translation="KRIKRLRRISGGDTYTIIYLKLLLLSLKDEGKLYYDGVESDFIK
                     ELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTEIPNLIGSETAWAEKKRRYR
                     QNKQRTLSLMSPTHVRQEIEIEKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYK
                     NLKEKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYD
                     VGESL"
     gene            1358..1519
                     /locus_tag="VUW50_001662"
     CDS             1358..1519
                     /locus_tag="VUW50_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983531.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            1611..3290
                     /locus_tag="VUW50_001663"
     CDS             1611..3290
                     /locus_tag="VUW50_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET7983532.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGCIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYHGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIDLDNDEQVECVSLLEKRS"
     gene            3558..4124
                     /locus_tag="VUW50_001664"
     CDS             3558..4124
                     /locus_tag="VUW50_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983533.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            4446..4634
                     /locus_tag="VUW50_001665"
     CDS             4446..4634
                     /locus_tag="VUW50_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET7983534.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            4696..5454
                     /locus_tag="VUW50_001666"
     CDS             4696..5454
                     /locus_tag="VUW50_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET7983535.1"
                     /translation="MVVYIRQSKLPSEVSINKYHAQVGAYLQGEEAVLYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVVF"
     gene            5512..6366
                     /locus_tag="VUW50_001667"
     CDS             5512..6366
                     /locus_tag="VUW50_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET7983536.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGCDSDTLAAITCSIAEAAYSIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            6478..7362
                     /locus_tag="VUW50_001668"
     CDS             6478..7362
                     /locus_tag="VUW50_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HET7983537.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            7525..9264
                     /locus_tag="VUW50_001669"
                     /pseudo
     CDS             7525..9264
                     /locus_tag="VUW50_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
     gene            9539..9994
                     /locus_tag="VUW50_001670"
     CDS             9539..9994
                     /locus_tag="VUW50_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983538.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNIIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
     gene            10439..10915
                     /locus_tag="VUW50_001671"
     CDS             10439..10915
                     /locus_tag="VUW50_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7983539.1"
                     /translation="MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            10915..11676
                     /locus_tag="VUW50_001672"
     CDS             10915..11676
                     /locus_tag="VUW50_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PezT"
                     /protein_id="HET7983540.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
     gene            11704..12480
                     /locus_tag="VUW50_001673"
                     /pseudo
     CDS             11704..12480
                     /locus_tag="VUW50_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12408..12860
                     /locus_tag="VUW50_001674"
     CDS             12408..12860
                     /locus_tag="VUW50_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983541.1"
                     /translation="MEFNINSKESSQQLDILYKEVGVLSLGQKVVAMLDFLLAYSDYS
                     KDFRPLIIDQPEDNLDNRYIYRHLVQQFRDVKAQRQIILATHNATIVTNSMTDQVVIM
                     ESDGVNGWIESQGYVSEKYIKNHIINQLEGGKDSFKHKMSIYETALSE"
     gene            13679..14038
                     /locus_tag="VUW50_001675"
     CDS             13679..14038
                     /locus_tag="VUW50_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983542.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSPFVRDKILM
                     TDDKQLAAKEWFSLWQSQKFEQISRDVHLVLIIARENHQVTQEHVSILLTCVQELIAE
                     VNQVQSLSRGFREKYMR"
     gene            14048..14413
                     /locus_tag="VUW50_001676"
     CDS             14048..14413
                     /locus_tag="VUW50_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HET7983543.1"
                     /translation="MVYRYRTNLKKVFLTDSELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYDQLVFELRRIGNNINQIARAINQSRLISQEQLQELSKGVGELIKE
                     VDKEFQVEVKRLKEFHGSH"
     gene            14400..16250
                     /locus_tag="VUW50_001677"
     CDS             14400..16250
                     /locus_tag="VUW50_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HET7983544.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLD
                     FPSYEEMVEMYNVNFTNNDKLYEYRNDRQEKHQQNIHAHHLIQSFSPEDNLTPEEINR
                     IGYETIMELTGGRFRFIVATHTDKDHIHNHILINAIDCNSDKKLIWNYALERNLRMIS
                     DRISKMAGAKIIEKRFSYRDYQKYRATSHKFELKQRLYFLMQQSKSFDDFLEKAEQLH
                     VHIDFSQKHSRFMMTDRAMTKPIRGRQLSKRDLYDEDFFRMHFTKQEIASRLEFLLNC
                     VNSLEGLLTKSKELNLTIDLKQKNVIFILEENGKQFSLSHKKISDEKLYDVNFFQDYF
                     KNKEVGVSEGIENLQAQYRAFQEERDKEKVSTEEIEEAFETFKEKRDAVHEFEVKLTE
                     HQIEKLVDEGIYIKVSFGINQSGLIFIPNYQLDIMEEENQKKYKVYIRETTSYFVYNK
                     EHSDKNQYIKGRTLIRQLTNDSRVIPYRRPTVERLQEKISEISLLIELTETDKKYQDI
                     KDNLVSEIAELDIKLTQTNEKIATLNKMAEVLINSKSEGSGSQKLARHEFSKLNMTES
                     TTLEQVNEELLKLQQEFGNVLDEYEKTIRKLGQLFKVFDECINKEIMNEI"
     gene            16506..17938
                     /locus_tag="VUW50_001678"
                     /pseudo
     CDS             16506..17938
                     /locus_tag="VUW50_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886556.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(18176..18310)
                     /locus_tag="VUW50_001679"
                     /pseudo
     CDS             complement(18176..18310)
                     /locus_tag="VUW50_001679"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         6648 a         2690 c         3765 g         5507 t
ORIGIN      
        1 acaaaaggat aaaaaggtta aggagaatat ctggaggaga tacctatacg ataatctacc
       61 taaaacttct actactaagc ctaaaagatg agggcaaact ctattatgac ggagtagaaa
      121 gtgattttat aaaagagtta gcactaacca tagatgaaac agacgatgat gtaatggtaa
      181 cagtaaatta tttaatcaat caaggcttat tagaagttgt aacagaaaat gatgaatact
      241 acctaactga aataccaaac ttaatcggat cagaaacagc ctgggcagag aaaaaaagaa
      301 gatacagaca aaataaacag aggacattgt ccttaatgag tccaacccat gtccgacaag
      361 agatagagat agagaaagat atagagatag ataaagagag agagggagag atagataaaa
      421 agtccacccc cattttttat ggagaattca aaaatgtaag gttaagcaaa gaagaatata
      481 aaaatctaaa agaaaaatta aactcacaca cagatataat gataaataaa ctatccagat
      541 acatggaaag cagtggtaag acctatcaaa accactatgt gacaatctta aaatggtatg
      601 aagaagataa agacaaacta agacagaaag gtttaaataa aaaaatgaat tatgatgtag
      661 gagaatcttt atagataaaa agaggtggaa aggcactatt aaagacttta agtttaaaaa
      721 ggtatcaaag tatgccagag ataaaaaaat ataaatatga cactttaaag gcgattagaa
      781 aattaaagaa atgctagaag acttttagat gacagttacc aagagggtct aattttcact
      841 gctacttatg cagtaggaat tggacctttt tatataagca aattctattt caaaattggt
      901 taccaagaaa gacttctttt cactgttaag tataagaggg taatttatag aattttgtta
      961 catcaagata aatattttct gatggtcaaa ttttaaaaag attaataact ctcgataaat
     1021 tttgaacctt aacaattgaa tagaccaaga caagacgtta attatttttg gagcgtaata
     1081 acctatccgt caagatcttt cggctgaata attcggcaaa aaaagttctg ctaagcaaaa
     1141 agaaagtgag cgataagtaa gagtttgaat atagggaggg ataccctatc cgctatgaag
     1201 aaaatgagga taatgatact tctaaggttg aagccggctc atccggaata gaggcggagg
     1261 tgagattcct atgttgctaa tccaaagcac ttgtcaatgt caaaaaactt aattaaacaa
     1321 ataattatta gatataaaac tttagaggag gtatatattg gacaatgact ggaatggatt
     1381 ggctgattta atagcaaatc tgattgcaaa atatgctggt gctttagatt tagataatct
     1441 acccgatcca acgccagcaa aaaatcaaga gatgaaaaat agttttgaca tggcaaaaac
     1501 acaaattgag acggactaat aaaagtgtta taatatactt gaaatgatag tccaaactta
     1561 aattaaaaaa attatatatg atatagaaaa attatattga ggttaatact atgtcaaaag
     1621 aaaaaataaa agtatacctc tatacacgag tatctacgtc aatacagata gaggggtatt
     1681 cgttagaggc acaaaaatca cgaatgaaag cttttgctat ttacaacgat tatgaaattg
     1741 ttggagagta tgaagatgcc gggaagtctg gcaagtctat tgaaggtaga atacagttca
     1801 atcgaatgat ggaagatata aaatctggaa aagatggagt atcttttgtt cttgtgttta
     1861 agttatcaag atttgcaaga aatgccgctg atgttctttc tacacttcag ataatgcagg
     1921 attatggagt taatcttatc tgcgttgaag atggcattga ttcatctaaa gatgctggga
     1981 aactaatgat ttctgtttta tcagctgtgg ctgaaataga aagagaaaac atccgtattc
     2041 aaacaatgga aggttgcatt caaaaagcaa gggaaggaaa atggaatggt ggctttgctc
     2101 cgtatggata taaacttgaa gacggcaagc tgtttataaa tgaggaagag gcagttgcaa
     2161 taagaacgat tttcgaccag tatgtaaata ctacgatagg agccaatggg atatctaaat
     2221 acttagagaa tcatggaatt agaaaaatcc caagacagaa tggtaagaat cctttgtttg
     2281 atgcaggtct tataagaaag atattaaaga atcctgtata tcatgggaaa atagcatttg
     2341 gaagaagaac tttagaaaaa gttcatggta caagaaatga atataagcag gttgaacaag
     2401 atgaatatct aatatctgaa gggatacatg aagctatagt ttccgatgaa gtttggcaag
     2461 ctgctcaggt taagctaaaa tctcaagcaa agaaatatga gcatgtgaat aaaggaaaag
     2521 atacacgcac acacttgctt tctggaattg taaaatgccc gatatgtgga gtgggaatgt
     2581 ttgggaacaa gtgtatcaag aaaaagaaag atggcacaaa gtataaagat ttttattact
     2641 atggttgtaa acataggcag atgataagag gtcataagtg tacattcagt aagcaaatta
     2701 gagaagaatt gttagatgat gctgttgcag aggtgattgt caagatagta agtaatccga
     2761 aatttgcttc tatgatgcaa gaaaaaatca acatgaaggt ggatacctct gaaatagaaa
     2821 aagaaataga taattaccaa aaggaattga gaaagagtca ttctacaaag tttaagctaa
     2881 ttgaggaaat agataattta gatgttgaag ataagcacta taagagaagg aaacaggatt
     2941 tagatgatag actatatcgt atgtatgaca aaatagatga attagaatca tcactaattg
     3001 atgcgaaagc aaagaaacag actattgaag ctgaaaaact tacaggagat aacatatata
     3061 aggttctgat ctattttgat aagctctata aagtcatgaa tgatgtagag cgtaggcagt
     3121 taatttcagc tttgatttct gaaattcaag tttatgaaga aaaacaatct aacggacaat
     3181 ggctaaaatc cattactttt aagctaccaa ttatagaaga aaatctcaac atagatttgg
     3241 acaatgatga acaagttgaa tgtgtaagtt tattagagaa acgtagttaa tcctcaaaag
     3301 gttccccaaa ctttttgagt cccactgacg catcacgctg aggcggtatc actgcttgta
     3361 cgagttgaag catcagcgaa gtaaaagtag atgttcagcc aatgcgacga ggtgagaccg
     3421 aaggtcgata aggtgtgttc tgggtatagg actcgtaaag taagtaggaa atatgatgag
     3481 atgatacaga gagcctgaat tggtcaggtg acgtgcttat ttacattagc ctgataatga
     3541 aaatggagag attatcgatg gttatattgg aattgtacca aaatgattat tctaaagatt
     3601 tagtagcatt tgattccata gaagatggga aagcctttgt ggctcaaatc ccgggataca
     3661 ctctggaaac agaagatggt tttgaagtgg agtattttaa tcctaaaaac ataccggatt
     3721 atatggaaat tatttttaac ggaaatattg ttcctttatc taaatttatg ttcgatcctg
     3781 aggaaaatgt aaacattatt tggaaagaga tttcaaattt atccctcaaa aatgatagag
     3841 tgatcgaagg atattcaaaa attgatgcat atgtcgttaa caaccatgaa gttaaagcct
     3901 atgtcgaaac aagagaaacg aactatcgta aagcaaaaga cttcttagaa agccgcggat
     3961 atgaaatcga cagaagcttt ttcggaagtg aagacggtga agcgattctt tacagaaaaa
     4021 aagggatcga agtttggcat ttcttgtgtc acttagaccc aatgtttgta gagattgaag
     4081 atgtagaagg atatgttaaa gaagaagttg gagagattca atagtcaacc aaagttcctt
     4141 gtgagtatat acctttgcta gtaaaagagt caaaacgtta taacgaaggg ggggaagaaa
     4201 acccttgagt gtaagtcttt ttcttgttta gagggaaagt attagagtag ccgctcataa
     4261 tttatactag atttgataac tgatttgaga cagattttat ttctataaat attgtgaaaa
     4321 ttttttaata ccaaagattt aaagttactg tgttaaaaat cctctccact tttaaactaa
     4381 tgtattataa aagccagttg acacaagtgt tacacctgtg atagaattaa cttgaggtga
     4441 tgaaaatggc aaccgataaa aatagaataa tgattagctt ggatgataaa aatctagaga
     4501 aacttgaaaa tttagtagaa gatgctagag atagaagggg aatgcgacta acaaagtccc
     4561 aggttattga attactccta aataccgttg attatttcga tgatattatg ggggctattt
     4621 actcaaaaaa ataagagcaa aattgctatt attttttaca aattggtgta acactagtga
     4681 tataggagtg atgaaatggt tgtttatatt agacaaagta aattaccaag tgaggtttca
     4741 attaataaat accatgctca agtaggtgcc tacttacaag gagaagaagc tgttttatat
     4801 caatcttttt ctgaaataaa agagttaaca agtgaagata tagttgtaga ttatataatg
     4861 gagactagag cattactaaa aatgatgggc ttaaacgttc cggttcatga ctatcctatt
     4921 gagcttaaag agttttatgg tcgaaagatt tatgctggta ttttaggaga gattgtgaat
     4981 atacctgata attggggaaa atttattaag cctaaagctg gttcaaaagt ctttactgga
     5041 agagttgtta atggaaccca tgatttaata ggtattggtc tacctttcga ctatcctata
     5101 tggattagtg aggttgtaga attcatagct gaatggcgtt gttttgtgtt agatggtcgc
     5161 gtattagatg ttcgacccta tacaggtgat tatcatgcac aatttgatgc aagtgtaatt
     5221 gatgaagcga tatcatgttg gaaagatgcg ccaatagctt atggactaga tatcggtgtt
     5281 actcgcgatg gcagaacact tgttgttgaa gtaaatgatg gttatgcatt gggaaattat
     5341 ggcctatctc ctttaaaatc aatcaatttt catagagcta ggtggaaaga aatggtaaaa
     5401 ccctattttg aaaaaaatga aatttttaaa atccaacagg atgttgtttt ctaaaataaa
     5461 tatggtatat aaccaatata aaaagcatca aaatgaacaa ggaggaatct gatgcttgga
     5521 gcaattattg gagatattgt cggttctgtt tacgaatgga acaatatcaa aacaaaggac
     5581 tttcctttat ttcggaagga ctgctttttt acagatgaca cggttatgac ctgtgctgtt
     5641 gcagaagcaa ttatgaatgg tggacaaaaa gacgatttca ttgacgcgat gaagaaatat
     5701 ggcagaatgt atccgaatgc tgattacggt gctcggttta atgcatggct aaacagcgat
     5761 aaccgtgagc cttataatag ctttgggaat ggatcagcta tgcgtatttc tccatgtgct
     5821 tggatcatgg actgtggttt ttatgcgaaa actggtatgt ggccatcatc tagaggactt
     5881 acgagtcttt ctgcagaggt aactcataat catccagaag gtgtcaaagg cgctatggct
     5941 acagctgatg ctatctttct atgtcgtttt tactttggtg gttattgtag agagtacgag
     6001 caatcaatta acgacaatcc tacagagtgt aaaagacgaa ttaaggatta tatagaaaaa
     6061 gaatacgatt acaatctatc tcaaacttta gatgaaatcc gtcctaacta tcgttttaac
     6121 gaaacatgtc aggaaactgt ccctcaagcc attatcgcct ttcttgaaag tagagacttc
     6181 gaagatgcga taagaaatgc catctcactt ggttgcgaca gtgatactct cgcagcaatc
     6241 acttgtagca tagcagaggc agcttacagt attcctgatt ggatcaagga taaggcctat
     6301 tcttacttgg atgaaccctt aaaggatgta gttaggcgat gggaaaatag aataaaagcg
     6361 tattaatctc cagtacgtca gatttctctt ttctgtattt tcattgtata ataggattac
     6421 atttacttgc atacgacgaa atgtaaacat ttctgtttaa aattaaggat aaaatcaatg
     6481 aaaaaagcaa tggtaattat caaccctact tctggtggcg agaaggcttt ggattacaaa
     6541 gaaaagctgg agaataaagc aaaagaatac tttgaatatg ttgaaaccaa aattaccgaa
     6601 aaagcgctgg atgcaacaca ttttgctgaa gaagcttctc gtgagcagta tgatgcagtg
     6661 gttgtgtttg gtggagatgg aactgtcaat gaagtcattt caggtattga tgagagagac
     6721 tacattccta agttagggat tatcccaggc ggtacgggta acctcattac aaaacttttg
     6781 gaaatcaatc aagacatcga tggcgcaatt gacgaactgg attttgattt aaccaataag
     6841 attgatatcg gtaaagcaaa tgacaactat tttggttata tctttagtat cggttctctg
     6901 cctgaggcga ttcacaatgt tgaaatagag gacaaaacaa aattcggtat tttaacctat
     6961 gctgtaaata ccatgaagtc tgtcatgaca gatcaggtct ttaacattaa ggttgagaca
     7021 gaaaatggaa attatgttgg tgaagctagc catgttttgg tccttttgac aaattacttc
     7081 gctgataaga aaatctttga agaaaacaag gacggctatg ccaacatttt gattctgaaa
     7141 gatgcctcta tattctccaa attatccgtc attcctgatt tattaaaagg ggatgttgtc
     7201 gcaaatgata atatcgagta tatcaaagcg cgtaatatta aaatctcttc agatagtgaa
     7261 ttggagtcag atgttgacgg agataaatca gataacctac ctgtagaaat caaagtccta
     7321 gctcagcgag tagaagtatt ttcaaaaccg aaagaggatt agtatataga gaaagccttt
     7381 tttaaggctt tttgtatact ttaaaagata gttcctttaa caacggacat tccttgcaaa
     7441 tagtttacaa aaatagtata ctggatttat tgagtttgaa aacgtttgcg taaaatttga
     7501 atgaatactt taggagacaa attgatggaa ttgagtgcta tttaccatag gcctgagtcg
     7561 gagtatgcct atctttataa ggataagaaa ctccatattc gaattcgaac taagaaaggg
     7621 gacattgaaa gcatcaactt gcactatggg gaccctttta tctttatgga ggagttttat
     7681 caggatacaa aagaaatggt caagataact tctggtacct tatttgacca ttggcaggtt
     7741 gaagtgtcag ttgactttgc acgtatccag tatctctttg agctcagaga tacagaaggt
     7801 caaaatattt tgtatggcga taaagggtgt gtggaaaatt ctctagaaaa tcttcatgca
     7861 attgggaatg gatttaagtt gccttatctt catgagattg atgcctgcaa ggttcctgac
     7921 tgggtttcaa atacggtatg gtatcagata tttcctgaaa gatttgccaa tggcaatgct
     7981 ctattaaacc cagaagggac tttagactgg gattcatctg tcacacctaa gagcgatgat
     8041 ttctttggtg gtgatttaca ggggattatt gatcatatgg attacttgca agacttgggt
     8101 attactggac tatatctttg tcccatcttt gaatctacaa gcaatcacaa gtacaatacg
     8161 acagattact ttgaaattga ccgtcatttt ggagacaagg agacctttcg ggaactggtg
     8221 gatcaagcgc atcatcgtgg catgaaagtc atgctggatg cggtatttaa tcatattggt
     8281 tcgcaatctc ttcaatggaa aaatgtcgtc aaaaatggtg aacagtctgc ttataaggat
     8341 tggttccata ttcaacaatt cccagtgaca actgaaaagc tagttaataa gagagactta
     8401 ccctatcatg tttttggttt cgaggactat atgcctaagc taaatacagc caatccagag
     8461 gtcaagaatt atcttttaaa ggttgcgact tattggattg aagagtttaa tatcgatgct
     8521 tggcgtttgg atgtggctaa tgagattgac catcagttct ggaaggattt tcgtaaggca
     8581 gttttagcta aaaatcctga tctttatatc ctaggagaag tctggcatac atctcagcat
     8641 tggctaaatg gagatgagtt ccatgccgtc atgaattatc ctttatctga tagtatcaag
     8701 gactatttct tacgaggaat taagaagaca gaccagttca tcgatgaaat caattgagag
     8761 tctatgtatt acaagcagca gatttcagag gtcatgttta atctcttgga ttcacatgat
     8821 acagagcgaa tcctgtggac ggccaatgaa gatgttcaac tggttaaatc agccttagcc
     8881 tttttctttt tacaaaaagg aacaccgtgc atttattacg gaaccgagct agccttgact
     8941 ggaggaccag atccagattg tcgtcgttgt atgccttggg aacgtgtatc aagtgacaat
     9001 gatatgctga actttatgaa gaggctgatt aagattcgga aatacgcgtc agtaatcatt
     9061 tcgcatggca agtatagcct tcaagaaatc aaatctgatc tagtagctct ggaatggaaa
     9121 tacgaaggac ggatcctcaa agtaatattc aaccaatcaa cagaagatta tcttttagag
     9181 aaagaagcag tagcactagc aagcaattgc caagaattgg agaatcagct tgtcatctct
     9241 ccagatggat ttgtgatttt ctaaaaacta gttgatgaag attatggtac atttcatatc
     9301 ttatatagta taataaggct agttactaaa cttgtaaagg agaacttaaa tgaattgtag
     9361 aggacatgaa acaagacaaa gaattgttag agattttgaa gtttagccta aagcacatat
     9421 taagctgtta gcaaatcaac aaaaacatag tgatgtagga gcaactattg aagatgaata
     9481 ttatgtattt atcgctgaga gtaaaattga tggcaagaag gaagttattc agtgttgcat
     9541 gggtgcggca agggattttt tagaactaat taatcacaaa gggctacctc tttttaatcc
     9601 gcttgtaggt gattctcatg taaataatag acaagaatat gacaatacag ggagtggaaa
     9661 tttacaacct gaaaagtgga atgaaactgc aaagcagctt tataatatta taatgtggtt
     9721 gattatttta tggaatgcta agccggatac acctttattt aattttaaag acgaagtaat
     9781 taagtataaa acatatgagc cttttgaaag cagtataaaa agagtaaata ctactataaa
     9841 gaatggtagt aaagggaaaa ctctgactga gatgattaat ggctacagag cggataacga
     9901 tattagagat gaaatttgta actttaatat tctgaaaaat aaaattcgtg atatgaaaga
     9961 ccaacaagga aatacaatgg aatcttactt ttagttattg ttgaattttg ggtattctat
    10021 aaaatatcct aattgagatt taaatagtag actatacaat atagttaaaa tatcagtaaa
    10081 aacaacactt tattgaggta ttggatacgc tttgctaata gcctaataat cacatgtgga
    10141 gtgttgctac aacgaaaaag gtgataatcc ttgatttcaa gctattttat aagcattttg
    10201 tctttgtaga taaaggcaat tttgacaata aaaatcctaa aaggtgaatc gttatagatg
    10261 tatttgtaga tatcgtttgc gcatcgaaaa aattaataca agaataaata tttatggctc
    10321 tttaggtgac ttttatagaa gtaaagttta ggatagaaaa acaagaaata acgcaccatt
    10381 tttggtgcgt tatgcttttt tatgctataa tggatttata aaaataaagg agtttgctat
    10441 gattggaaag aacataaaat ccttacgtaa aacacatgac ttaacacaac ctgaatttgc
    10501 acgaattata ggaatttctc gaaatagctt gagtcgttat gaaaatggaa ctagttcagt
    10561 ctctacggaa ttaattgaca tcatttgtca gaagtttaat gtatcttatg tcgatattgt
    10621 aggagaagat aaaatgctca atcctgttga agattatgaa ttgactttga aaattgaaat
    10681 tgtgaaagaa agaggtgcta atctattatc tcgactctat cgttatcaag atagtcaggg
    10741 aattagcatt gatgatgaat ctaatccttg gattttaatg agtgatgatc tatctgactt
    10801 gattcagaca aatatctatt tagtagaaaa ttttgatgaa atagagagat atagcggcta
    10861 tttggatgga attgaacgta tgttagagat atctgaaaag cggatggtag cttaatggaa
    10921 atccaagatt atactgatag tgaattcaaa catgctttag caaggaatct tcgttcactg
    10981 acaagaggaa aaaagtccag taaacaacct atagcgattt tgcttggagg tcaaagtggt
    11041 gccggtaaga ctacaattca tcgtattaaa cagaaagaat ttcaaggaaa tattgttatc
    11101 atagatggtg atagttttcg ttctcagcat ccacactatt tagaactgca gcaagaatat
    11161 ggcaaagaca gtgtagaata taccaaagat tttgcaggaa aaatggtaga gtctttagta
    11221 acaaaattga gtagtttgag atacaatctt ttgatagagg gaactttacg aacagttgat
    11281 gttccaaaga aaacagcaca actcttgaaa aataagggat atgaagtaca attggcctta
    11341 attgcgacaa agcctgaatt gtcgtatcta agtactctta tccgttatga agaactgtac
    11401 attatcaatc caaatcaagc acgcgcaact ccaaaagaac atcatgattt cattgtaaat
    11461 catctagttg ataatacacg acaattggaa gaactggcta tctttgaaag aattcaaatt
    11521 taccaacgag atagaagttg tgtatatgat tcaaaagaaa atacaacttc agcagcagat
    11581 gttcttcaag agttactgtt tggggagtgg agtcaggtag agaaggagat gttgcaggtg
    11641 ggggaaaaga gacttaatga attacttgaa aaataaacaa ttgatatttt taggagagta
    11701 gaaatgagag ggtttaataa caagataaag tctgtttatc aagaactaac aaattccaaa
    11761 gagaaattcg gtagctttca caagacttta attcatttgc atacacctgt ttcttatgat
    11821 tacaagctat tttctaattg gactgcaacg aaatatagaa aaattactga agatgaacta
    11881 tatgatatat tttttgaaaa taagaaaata aaagttgata agacaatttt tttagtaatt
    11941 ttgataaggt tgttttttct agttcaaaag aatatattag ttttcttatg ttagcagagg
    12001 caatcataaa aaatggaata gaaatagttg tagtaactga tcataatact accaaaggta
    12061 ttaaaaagtt acaaatggca gtctcaatca taatgaaaaa ttatccgatt tatgatatac
    12121 atcctcatat tttacatgga gtagaaatta gtgcagcaga taaattgcat attgtatgta
    12181 tatatgatta tgaacaagaa tcatgggtta atcaatggtt aagtgaaaat attataagtg
    12241 agaaagatgg aagttatcaa cattcactga ctataatgaa ggatttcaat aatcaaaaaa
    12301 tagttaacta tattgctcat ttcaatagtt atgacatttt gaaaaaaggt tctcacttat
    12361 caggtgcata taaacgaaaa attttttcta aagaaaatac acgatttttg gagtttaata
    12421 ttaactcgaa agaatcttcg caacaacttg atattctcta taaagaagtt ggtgtattaa
    12481 gtttgggaca aaaagttgta gccatgcttg attttttatt agcatatagt gactattcta
    12541 aagacttcag accattgatt attgatcagc ctgaagacaa tctagacaat cgttatattt
    12601 acaggcattt agttcagcag tttagagatg tgaaagctca acgtcaaatt attttagcaa
    12661 cacataatgc tacaattgta acaaattcta tgacagatca agttgttatt atggagtcag
    12721 atggagttaa cggatggatt gaatcacagg gatatgttag tgaaaaatat ataaaaaatc
    12781 atatcatcaa tcaattagag ggaggaaaag attccttcaa gcataaaatg tctatatatg
    12841 agacggcttt atcagagtag agtcagaaaa agtaggttag aaatttagcc tacttttttc
    12901 tttgttcgac aggcatagtg tacatctgag gtccaagtcc tctgtggata tttgctgcag
    12961 atgaaaccaa tagcgactcc taagcctgaa tatcgtgagg taggggggat aggaaggaat
    13021 tagcgaaatc aaggttctac aaacagaatc gtgacttgaa gccatatata gcggatgagg
    13081 aactctaaaa tccaaatagg tgtcgtaacc tatatacgta aattacgaga gtaaactagg
    13141 aaagatgtac ggcttattcc gtgagcgttt aggacgtagt acaacgaatc atgggagtca
    13201 gctgaacaca tagtattgaa gaaatttctg taatggaaat ggagcgaaga agtgaacaat
    13261 taaatgaata cctctctaat taaatttgtc aattctaatt cctggtatga aaagacagtg
    13321 acctaaaaat gtaaacgatg ggagctgatc ataaatatag gacggtacat gcagtggtgt
    13381 tagagattag tccctacttg atttgtgata acttccccaa ctttcttctg ctatactttt
    13441 ctcaactttt aaaaatccaa ctaagaattt tacctgggag tttgggggcg tagccaccaa
    13501 gttatcttat ggttagctgt caaaactgga aggttttggt tagctggcga tatgattttt
    13561 tgggtattgt gaccacaatg cctgagctcg caaagaccga acaaggactt taagaaattt
    13621 cccgtatgac acacggggtc ggggtttacc cgagaaagga tgaaagtgag gaaaagagat
    13681 gtctgaacag taccgtgaca ttcgaaaaga ggtaaatcta accgcagatg agctaaaaca
    13741 gattgaaaaa atgatggagg ttgataatta tcgtcacttt tcaccattcg tcagggataa
    13801 aattttgatg actgatgaca aacagttagc tgccaaagaa tggttttccc tttggcagtc
    13861 tcaaaagttt gaacagatca gtcgagatgt gcatttggtt ttgattatcg caagagagaa
    13921 tcaccaagtg actcaagaac acgtgtcaat cttattgacc tgcgttcaag aattgattgc
    13981 ggaagtcaat caagtacagt cactcagtcg tgggtttcgt gaaaaataca tgagatagga
    14041 ggctactatg gtttatcgtt atcgtaccaa tctcaaaaaa gtatttctaa cagattcaga
    14101 attacaccaa ttgaatgaac ggattgctaa gagcaattgt caaaattttt cagtctatgc
    14161 cagaaaagtt ttgcttaatc ccaatatgtc atttgtcacg attaacacgg atacttatga
    14221 ccagttagtt tttgaattga gacggatcgg aaataacatt aatcaaattg cgcgtgctat
    14281 caatcaaagc cgtctgattt ctcaggaaca gttacaagaa ttgagtaaag gagtcggaga
    14341 gttgatcaag gaagtggaca aagaatttca agtggaagtg aaaagattga aggagtttca
    14401 tggtagtcac taagcatttt gcgacacacg gaaaaaaata tcgtaggcgt ttaattaagt
    14461 atatcctcaa tcctgataaa acggacaatt tgaaattagt atctgatttt ggtatgagca
    14521 attacttaga ctttcctagc tatgaagaaa tggtagaaat gtacaatgtc aactttacca
    14581 ataatgacaa gttgtacgaa tacagaaatg accgacaaga aaaacaccaa caaaatattc
    14641 atgcccatca cctcattcaa tcattttctc ctgaagataa tctgacacct gaagaaatta
    14701 accgcattgg ttatgagacc attatggaat taacaggagg ccgttttcgt ttcatcgtag
    14761 cgactcatac agacaaagat catattcata atcacatctt aatcaacgcc attgactgta
    14821 attcagataa aaagttgata tggaattatg ccttggaacg aaaccttcgt atgatttcag
    14881 accgcatttc taaaatggct ggggcgaaaa ttattgaaaa gcgtttctct taccgtgact
    14941 atcaaaaata tagagcgact agccataagt ttgaactaaa gcaacgtctt tattttttga
    15001 tgcagcagtc aaaatccttt gatgattttt tagaaaaagc agagcaatta catgttcata
    15061 ttgattttag tcagaagcat agtcgattca tgatgacgga tcgggcaatg acaaaaccaa
    15121 ttcgaggacg tcaactcagt aaacgagatt tatatgatga agattttttt agaatgcatt
    15181 ttaccaagca agagattgca agtcgtttag aatttttgtt gaactgtgtc aattctttag
    15241 aagggttact aacaaaatca aaagaattga atctaaccat tgacttaaaa caaaaaaatg
    15301 taatctttat acttgaagaa aatggaaagc aattcagtct gagtcataaa aaaataagtg
    15361 atgagaaatt atatgatgtc aatttttttc aagattattt taaaaataag gaagtaggtg
    15421 tttcagaagg aatagaaaat ctacaggcac agtaccgtgc ttttcaagaa gaacgagata
    15481 aggagaaggt atctactgaa gaaattgagg aagcctttga gacatttaag gaaaaacgag
    15541 atgccgttca tgaatttgaa gtaaaactta cagaacacca aattgagaag ttagttgatg
    15601 aggggattta tatcaaagtg tcctttggta taaatcagag tggtcttatt ttcattccta
    15661 actatcaact ggatattatg gaagaagaaa atcagaaaaa atataaagtc tacattcgtg
    15721 agacaacctc atattttgtc tataacaaag aacattcgga taagaatcag tatatcaaag
    15781 ggagaacctt gattaggcag ctaaccaacg atagtcgagt aataccatac agaagaccga
    15841 ctgttgaaag acttcaagaa aagatttctg agattagcct cttgattgag ttaactgaga
    15901 cagataagaa ataccaagac attaaagaca acttggtttc cgaaatagca gagctagata
    15961 taaaactgac tcaaacaaat gaaaaaattg ccaccttaaa taagatggcg gaagtactta
    16021 tcaattctaa gagtgaaggt tcgggtagcc aaaaactagc taggcatgaa ttttctaaat
    16081 tgaacatgac agaatcaaca actttagaac aagtcaatga agaacttttg aagttacaac
    16141 aggaatttgg taatgttctt gatgagtatg agaaaacaat aagaaagctt ggacaattat
    16201 ttaaggtttt tgatgagtgt atcaataaag aaattatgaa tgaaatataa atcagactga
    16261 agacaaactc aaattttttt ggatatcttt tctgtttatg gttgccaata tcggtttaac
    16321 ttctaaaaca aacattaata aaaatgcatt caaagacgat tttacctatt ttacgattac
    16381 gcttcgaccc caaatcatta aagttgtcta tgctctatca ttgcatcact ggacttgtca
    16441 agagttaaga taattcggag tccactgaaa aagtcatcgt atgatggtat actaaagatg
    16501 aggtgatggt atgcttgata atcgatgtta gtccctattc tactctgtgt gatattgttg
    16561 ttcccaaaac tcatttttta cgacagctaa tggaactctg tgattttagt tttatttatg
    16621 acgagctgga aaagaattat caacctgact tcggatgtcg ttcctactct ctccttatca
    16681 tgatgttcaa gtatctctta ttaaaagaca tctataagtt gtccgatgtg gatgtggtgg
    16741 agcgctcctt ttctggtatg accttcaaat acttccttgg tctagctcct gttattgaac
    16801 cgtcctcatt gactaaattc cgaaaacttc ggaacaaaga tgaacgatta cttgatttgc
    16861 tcatcgctaa atctgttcag attgcgatag agctcggtct gattaaaagt aacattctca
    16921 ttgtggatgc gactcatacc aaagttcatt acaatcacaa gaaacctcaa gaagtcctcc
    16981 gagaacgctc aaaagccttg cgtaaaacga tttatcagta ttcggaatat atcaaggcag
    17041 aatttcctag taagcctcag gaagatactc tagtagctga attaagatat acacaagaag
    17101 taatatcagt ccttgagaag catgacgaat tgactggaat accagctatc tcacaaaatt
    17161 caattacctt aaagaagctg tagaggatga cttagagcac ttggaatcct ctgtcaaaga
    17221 agaagcaaga attggacata agtcagccga tagttccttt tacgggtaca aagagcattt
    17281 tgccatgacc gatgaacgga ttattactgc ctgtgtggtg acatcgggtg aaaaaagtga
    17341 tggtcctgtt ttagaagaac tctatcacaa atctaaggat aatggcgtga cgattgaagc
    17401 tattgttgga gatagggcct actctgggaa ggataatatg caatttacca agaaagaacg
    17461 tgtgcattga gtcgctaggc catatccaac tatttcaaaa ggctttcgta ggaaggaaga
    17521 tgagtttttc tataacaagg acgcaggtct ctttgtttgt ccagaagggc atatggcact
    17581 aaagaaggaa aagacaggaa gaaggacggc tcaggaaacc cactattttg acatcaagca
    17641 ctgtcaaata tgcccttcaa aagtgggctg ttataaagaa ggggctaagt ctaagactta
    17701 tttgataccc cttaagagcg atgaacatat tttccagaag aggtttcagg aagcgcccta
    17761 ttttaaagaa aaggccaaac atcgctacaa gatagaagcc aaaaatgcgg aacttgaaaa
    17821 cagacatggt cttaatgtag ctagagcacc aggtctttta aacatggagt tacaagccgc
    17881 cacaaccata tttgtggtta atatgaagag gattataact ctaataaacc aaaaataagg
    17941 aaaagcaaag aggaatctat ccaagcagga gatagatccc tcttttttca ttttagttga
    18001 actgtagagc ttactttttc agtggactcg atgattagtg actatttttt tcaaactcta
    18061 ttagttattg gtaccctaat gccgaatgga gatatttagg attgtaatag atttcaatat
    18121 atttaaagat ttcgttcaat agttgcgaaa ttggcatcat tgacaagttc tctttttatg
    18181 ttttgtaaaa ggattccatg ggtgcattat catatggatt gccttttcga ctcaggctag
    18241 acttgcatct tttctgttta aggagttctt gaaatctagc tccagtatat tgagatcctt
    18301 gatcggtatg gccgactagt tcttatttga gattttctct atgataagct tgattgaaaa
    18361 cattcgttac caacttatct tgcattcgat ttcccattgc ctaaccaaca atttttctgc
    18421 tataaacata tatataatga taaataaaga gtacctgctt ttgtcggaat ataagttaaa
    18481 tcccccagcc agatttcatt tttacctttt gtttgaaaac actgattaat caaattgggt
    18541 cgtgttaatg aggaatgccg acggctatag tacttgtatt tatagcggat tcctttagca
    18601 tagagacaaa
//
LOCUS       DAVDWT010000037        18428 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVDWT010000037 DAVDWT010000000
VERSION     DAVDWT010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18428
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.037"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            32..292
                     /locus_tag="VUW50_001680"
     CDS             32..292
                     /locus_tag="VUW50_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983545.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELELVKSTILQNDDISLDQNEIFEILKNFFDG"
     gene            complement(1057..1456)
                     /locus_tag="VUW50_001681"
                     /pseudo
     CDS             complement(1057..1456)
                     /locus_tag="VUW50_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1581..2039
                     /locus_tag="VUW50_001682"
     CDS             1581..2039
                     /locus_tag="VUW50_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HET7983546.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            2052..2660
                     /locus_tag="VUW50_001683"
     CDS             2052..2660
                     /locus_tag="VUW50_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983547.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            2731..3063
                     /locus_tag="VUW50_001684"
     CDS             2731..3063
                     /locus_tag="VUW50_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983548.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            3074..4489
                     /gene="ply"
                     /locus_tag="VUW50_001685"
     CDS             3074..4489
                     /gene="ply"
                     /locus_tag="VUW50_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HET7983549.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            5370..6086
                     /locus_tag="VUW50_001686"
     CDS             5370..6086
                     /locus_tag="VUW50_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7983550.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            6210..6679
                     /locus_tag="VUW50_001687"
                     /pseudo
     CDS             6210..6679
                     /locus_tag="VUW50_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            6696..7517
                     /locus_tag="VUW50_001688"
                     /pseudo
     CDS             6696..7517
                     /locus_tag="VUW50_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601671.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            7524..8555
                     /locus_tag="VUW50_001689"
     CDS             7524..8555
                     /locus_tag="VUW50_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7983551.1"
                     /translation="MGFLLMGALFIVLPRTMVSAKRINQVLDLHSSIQNPVQVQLTDE
                     NFKGQVEFKDVTFRYAANSEAVIEHVSFKAETGQTVAFIGSTGSGKSTLVNLIPRFYD
                     VSAGEILVDGVNVQDYDFSATAHAGQKVAIVGPTGAGKTTIVNLLMKFYEIDKGSIRI
                     DGVDTKAMTRSEVHDAFSMVLQDTWLFEGTIRDNLIYNQIGISDERMMEASKAVGIHH
                     FIMTLPDGYDTILDDTVTLSVRQKQLLTIARALLKDAPLLILDEATSSVDTRTEELIQ
                     KAMDRLMEGRTSFVIAHRLSTIRNADLILVMKDGNIIEQGNYEELMAQGGFYADLYNS
                     QFTEDEAEE"
     gene            8621..8983
                     /locus_tag="VUW50_001690"
     CDS             8621..8983
                     /locus_tag="VUW50_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HET7983552.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            8980..9483
                     /locus_tag="VUW50_001691"
     CDS             8980..9483
                     /locus_tag="VUW50_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7983553.1"
                     /translation="MKNYQEWYRNIRSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWNICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            9597..10043
                     /locus_tag="VUW50_001692"
     CDS             9597..10043
                     /locus_tag="VUW50_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7983554.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELEAILPRYFSRVSKSTI
                     VNIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            10048..10722
                     /locus_tag="VUW50_001693"
     CDS             10048..10722
                     /locus_tag="VUW50_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983555.1"
                     /translation="MKKKAFGIVLLVLAALILVQGNFGIPSLGGHIWPLIGIAFFAYQ
                     SVGALLRRHLTSASFTALVALMIANHFYGILPIPNQTLIWAGVLIVLGVNMLTHSNRT
                     WNGKKWWYDGEKNILIDKEVAFGTGTFYKQDQELVEDQVEVGFGNAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYAK"
     gene            10883..11074
                     /locus_tag="VUW50_001694"
     CDS             10883..11074
                     /locus_tag="VUW50_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HET7983556.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            11359..11658
                     /locus_tag="VUW50_001695"
     CDS             11359..11658
                     /locus_tag="VUW50_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HET7983557.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            11655..11972
                     /locus_tag="VUW50_001696"
     CDS             11655..11972
                     /locus_tag="VUW50_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET7983558.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGISSLVVLEKDKEIGRFVNRDCKSKEQINDFLA
                     GLK"
     gene            11988..12614
                     /locus_tag="VUW50_001697"
     CDS             11988..12614
                     /locus_tag="VUW50_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HET7983559.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKANGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            12647..13408
                     /locus_tag="VUW50_001698"
     CDS             12647..13408
                     /locus_tag="VUW50_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7983560.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            13486..13881
                     /locus_tag="VUW50_001699"
     CDS             13486..13881
                     /locus_tag="VUW50_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET7983561.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            14151..14435
                     /gene="groES"
                     /locus_tag="VUW50_001700"
     CDS             14151..14435
                     /gene="groES"
                     /locus_tag="VUW50_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET7983562.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            14451..16073
                     /gene="groL"
                     /locus_tag="VUW50_001701"
     CDS             14451..16073
                     /gene="groL"
                     /locus_tag="VUW50_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET7983563.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEVTGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            16211..16966
                     /locus_tag="VUW50_001702"
                     /pseudo
     CDS             16211..16966
                     /locus_tag="VUW50_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            17412..18185
                     /locus_tag="VUW50_001703"
     CDS             17412..18185
                     /locus_tag="VUW50_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7983564.1"
                     /translation="MIDKVIEQYKSHDNLDIRVELHKKYSKNKLGFNNWIFSNYQITD
                     EVKVLELGCGTGELWKSNSDSIDKMKQLIVTDFSKDMVKSTKSVIGNRDNVNYEIMDI
                     QKISFENETFDIVIANMLLHHVNDIPKALSEVNRVLKTEGIFYCATFGENGVVNYLAS
                     LFKDEVNQDLENRTFTLQNGKRYLSRCFNSVDTLLYDDELQVTSIDDLVKYIQSFKGI
                     SEIGSLEEEIIRKRLESEFNNSMSIIPKEYGMFIARKES"
     gene            18198..>18428
                     /locus_tag="VUW50_001704"
     CDS             18198..>18428
                     /locus_tag="VUW50_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7983565.1"
                     /translation="MKNYGEAFRYFRKLNGYSLEYAAVDSISKSQLSRFERGENEISL
                     STFFELLSNINVSIENFCNHLEHYKRSERDDFL"
BASE COUNT         5618 a         3241 c         4113 g         5456 t
ORIGIN      
        1 aattattttt aaggctaaag cttggctgga tatgagggaa cgctctgcta caggtgctca
       61 aggtttaagt aagtccatta aaaagcattt gaatgacctt acccgtttga cagcttcctt
      121 gctaggagat gaaaagttat cggctataac atcaagtagt gcggtaaaag cagacatgca
      181 ccgctttgtg atagaattag agcttgtgaa gtcaactatt cttcaaaatg atgacatttc
      241 attagatcaa aatgaaattt ttgaaattct gaaaaatttt ttcgatggtt aaaataattg
      301 tagcgagatg gctatattga attcgtctat acctggaaac tagaaaaaac ttcaatttca
      361 ggagaaaatg aagtcaatct tcccacaatc aaacgtatag tatcaaggtt tttcaagacc
      421 tgatattatg cgttttttgc ttttcaaaac tttttgccta gtctttgttt ttatcctcta
      481 gtcacttgat ttgtttcagg tggtttttta gtatagtaaa atgaaacgag aacaggacaa
      541 attgatcagg acagtcaaat cgatttctaa caatgtttta gaagcagaag tgtactattc
      601 tagtttcaat ctactatagt taaatctgcg gtcaagtcta ctggtgaatc tatgattgta
      661 atactcttcc aaaatctcat caaccacgtc agtcttgcct tgcagtctgt atcttactga
      721 ccaagctagt gatggactta gaataggtga tttggagcgt cctattggct aggaaatgct
      781 gatcatagtc ctttgctgag gctagggtgt ttcaacattc aacactcaac tggttgatct
      841 agttgatagg aagggaatta ctataaaata ctcagtcttc catcatattt tttgaaacga
      901 ttgtgtaatc aaaatgtacc aattattgta gtattggtac agaagatgtt gtgaatggat
      961 aaatatatca taactgctgt ctctctatgt aaaaagaagg ctgtatcaaa aagatttcat
     1021 atgtctgtgc atatatataa tagacttcct gcaaaactag aatcctagtt catgattgat
     1081 aataccagca ctcaaattca ttcgtaatcc gaagcgttta cgatgatttc gacaggttgt
     1141 tgaaaacatt ttaaacgttt ttactttggc aaagatgttc tcaaccttgc ttctctcctt
     1201 agatagcgca tggttataga ctttatcttc aactgttagt ggcttgagtt tgctggattt
     1261 acgtggagtt tgtgcttgag gatatatctt catgagccct tgataattac tgtcagccaa
     1321 gattttacca gcttgtccga cttttttgat aagctgtttt taatacagct aacatctctt
     1381 caaaagtggt acgctgaaca ccaacaagac gcttaaatcg tgtatcagtt aattgtttac
     1441 ttgcttcata attcatagaa ctactatacc atattttgtt tcgcagggag tctaataagt
     1501 tatagtccag tttttactat actttgatat tcaaatatgt tatactatgg ttatcaaagt
     1561 aaatgaaagg agttacgaat atgacgccag aagaaatgta cctgacagag cgattagacg
     1621 tacagatagc tcatttttta aagaaaagcg ttcaacatcg taggcgctat aaggtattaa
     1681 aaataacaga aatcgtggca ggtttcctca tagctgtctt ttgtgctatt cctatgccag
     1741 gtgatcgcta ccgtttgatt tcggttgcct tatccagtct cggcttgctg tgtgagggga
     1801 ttatcaattt gtataatgca aaggaaaatt ggatttctta ccaaaaaact gcgcaactcc
     1861 tggagaaaga aaaattcctc tatcaatgcc aaacggagaa atatgcagga aagaccaagg
     1921 cttttgccct atttgtcaag acatgcgaag gtcttatctc agaggagatt aaccagtggg
     1981 aaagtatcca gtcaaaagaa gtggcagcta gtgcagatgc tccagttaaa aaagagtagg
     2041 aggtagagga aatgtctcaa tccagctacc tgtcgccctt gctctggtta aaaaaagaag
     2101 ccgataagga aaagatgagc gcgacccagt gccagatatt tttcttttac tatcaaatgt
     2161 ttgagctctt atttgctaga gaaagcgaca tgaaagactt atgtctggga acgaaaggtt
     2221 tttatttctc gcagttagag aaaaatttgc tttctggagt ttcccgattt ctaaaaaact
     2281 tggaggggaa agtaactctc aaggctaacc aagaagtatc agctcgcaaa gccctttttc
     2341 tagccttgac aactagccaa tcagattggc aggagttagc tcctgttttt gatttttatc
     2401 agactatcgg gaggcttgaa aatccttctc tcttgagttc tcaggacaga caacatctga
     2461 tgtggattta ccagtcagct ttggagaagg attatattgt caaggttatt ggcgacaagc
     2521 attttgtatt gaagagacaa gatgctacta aattgacagc gcgccaaact caaactttgg
     2581 aaattctgag tcaatcagaa gacttggtca atcctgtcta tgttacatta ggagaaaagg
     2641 gggtgctctt gcttgattaa gagaggagat gttgtagctc tttatttgcc ttttccgact
     2701 attagtagcg atttggctgt gaagaatcat atgtatatct gtattgacaa cagcatgact
     2761 aaaaacaaag agctggttaa aaatcagacc ttcaaaccag ctctcttgac cagacgtttg
     2821 gtcaagaact ttatgataga agagccggat ctagctcgta atccttttac aagaccaacc
     2881 ttgattgact tagataaggt atttatgttg gataatacgg tcattccgac ttcttattta
     2941 gccagacggc gacgcaatgt ctcagaagaa ttgtacgagg aaattttgga ttacttagtc
     3001 caaccacggc tgatttcgct gaacaagtct gagtttatgc aactcaatcc aggaacttat
     3061 taggaggtag aagatggcaa ataaagcagt aaatgacttt atactagcta tgaattacga
     3121 taaaaagaaa ctcttgaccc atcagggaga aagtattgaa aatcgtttca tcaaagaggg
     3181 taatcagcta cccgatgagt ttgttgttat cgaaagaaag aagcggagct tgtcgacaaa
     3241 tacaagtgat atttctgtaa cagctaccaa cgacagtcgc ctctatcctg gagcacttct
     3301 cgtagtggat gagaccttgt tagagaataa tcccactctt cttgcggtcg atcgtgctcc
     3361 gatgacttat agtattgatt tgcctggttt ggcaagtagc gatagctttc tccaagtgga
     3421 agaccccagc aattcaagtg ttcgcggagc ggtaaacgat ttgttggcta agtggcatca
     3481 agattatggt caggtcaata atgtcccagc tagaatgcag tatgaaaaaa tcacggctca
     3541 cagcatggaa caactcaagg tcaagtttgg ttctgacttt gaaaagacag ggaattctct
     3601 tgatattgat tttaactctg tccattcagg cgaaaagcag attcagattg ttaattttaa
     3661 acagatttat tatacagtca gcgtagacgc tgttaaaaat ccaggagatg tgtttcaaga
     3721 tactgtaacg gtagaggatt taaaacagag aggaatttct gcagagcgtc ctttggtcta
     3781 tatttcgagt gttgcttatg ggcgccaagt ctatctcaag ttggaaacca cgagtaagag
     3841 tgatgaagta gaggctgctt ttgaagcttt gataaaagga gtcaaggtag ctcctcagac
     3901 agagtggaaa cagattttgg acaatacaga agtgaaggcg gttattttag ggggcgaccc
     3961 aagttcgggt gcccgagttg taacaggcaa ggtggatatg gtagaggact tgattcaaga
     4021 aggcagtcgc tttacagcag atcatccagg cttgccgatt tcctatacaa cttctttttt
     4081 acgtgacaat gtagttgcga cctttcaaaa cagtacagac tatgttgaga ctaaggttac
     4141 agcttacaga aacggagatt tactgctgga tcatagtggt gcctatgttg cccaatatta
     4201 tattacttgg gatgaattat cctatgatca tcaaggtaag gaagtcttga ctcctaaggc
     4261 ttgggacaga aatgggcagg atttgacggc tcactttacc actagtattc ctttaaaagg
     4321 gaatgttcgt aatctctctg tcaaaattag agagtgtacc gggcttgcct gggaatggtg
     4381 gcgtacggtt tatgaaaaaa ccgatttgcc actagtgcgt aagcggacga tttctatttg
     4441 gggaacaact ctctatcctc aggtagaaga taaggtagaa aatgactagg agaggagaat
     4501 gcttgcgaca aaaagaggcg atgatctctc tgcggatatc tgaaagttta tctcttgcct
     4561 agcgatttcc attggagtat gctcgtgatg ttaaaatgaa tctatcaaac agaatgtgta
     4621 aaaaagtaaa aagggtagcc aattccttga tatacagggg attgattatc ctttacttgt
     4681 tttagatgat tggaaattca agaacgacac gtatcggaaa tattttgctg tttatatcta
     4741 atacccattt atggaaaaaa tgaaattaaa tatgaacaaa tcaattgcta gcaatgtttt
     4801 agaagtgcca gtgtactagt ctaacttctc catcttggga acatgtaaat tatactcttc
     4861 gaaaatctct tcaaaccatg tcagccccat ccgcaacctc aaaacagtgt tttgagcaac
     4921 ctgaggctag ctttctagtt tgctctttga tttttattga gtattagagg tttgcttaga
     4981 aactcttcta ttgatttctt agaatacaat agcgcctata agaactgttg aatttcaaag
     5041 tatttacttg aactatgata tgtctggagt agactgtcaa caaaaggtgc agtatgattg
     5101 tttttgtcgc ttatatctac aaacttcaaa cagtaatttg agctggcttt gtcaaacttg
     5161 gctgaaactt tctatttgca gataatggtc tagtttgttt tttgaatgtg gatgagtgtc
     5221 tgttgataaa gatagaaagg caagaccatc ttagcaggta aagtgctatc ctagcttgat
     5281 ttgctaataa aatccatttc ctctagtgaa aatcaaaaaa acttgtgtta taataagaaa
     5341 gattaaaatg tgaaaaaagg agattcctaa tgggacgtaa atgggccaat atcgtagcca
     5401 agaaaacggc taaagatgga gctaactcta aagtatatgc aaaatttggt gtagaaatct
     5461 atgtagcagc taaaaaaggt gatccagatc cagaatcaaa ctcagctttg aaattcgtta
     5521 tcgaccgtgc taaacaagcc caagtgccaa aacacattat cgataaagcg attgataaag
     5581 ccaaaggaaa cacagacgaa acctttacag aaggacgtta cgaaggtttt gggccaaatg
     5641 gctctatgct aattgtggat actttgactt ctaacgttaa ccgtacagca gccaatgtcc
     5701 gtgcagcctt tggtaaaaac ggcggaaaca tgggcgcttc aggttctgtt tcttaccttt
     5761 ttgacaacaa aggtgttatt gtatttggag gtgaagatgc ggacgcagtc tttgagcaat
     5821 tgctcgaagc ggatgtggat gtggatgacg tagaagcaca agaaggtaca atcacagttt
     5881 acacagcgcc aactgacctt cacaaggcta tcgttgctct ccgtgagtct ggtattgaag
     5941 aattccaagt gactgaattg gaaatgattc ctcagtcaga agtggaattg tcaggcgaag
     6001 accttgaaac ctttgaaaaa ctttacagcg ttcttgaaga cgacgaagac gtccaaaaga
     6061 tttatacgaa cgtagatggg ttttaattta acctatagaa catgatccta agtgagaaaa
     6121 tcacttggga tttttttaat caagaaaata gttctcatag aaatttaatt attctccaga
     6181 gaactatttt tatttagaag ggggagatta tggataaacc gatgttagtc tttaagcgtt
     6241 ttggtcatca aatttacctg atggtgcaaa aggaagccaa acgttgcggc attgaattta
     6301 tgggtgggcc gcaaggtcag gttgtgcgtt ttttggataa tcgcgagaaa aaccaagact
     6361 tggtcttgat taaagatatc gagcaggaac tcaatattac caagtctgtt gctagtaatc
     6421 tggttaagcg tatagtgcaa aatggtttgg tggaattgga ggcgagtcct gttgataagc
     6481 gggctaagtt tgttcgtctg acggacaaag cacgttctca gatgcaacag gttaaggctt
     6541 ttttgaacgc atagacaagt agttgatgga agacattgat gaagatgaat tactgatttt
     6601 tgagaaagtt ctcggtcaac tacaggcaaa tatcaaggga ataggaggag agaataaaga
     6661 aattagccaa aagaattagt agaaaagaat gggggatgat tttactagcc attctcttta
     6721 cttgcttttc ggtctatcta gagttggaag tgccgaccta tatctcgaaa attacggatt
     6781 tgctaggtag tcaaggaact aatttagatg agttgtggca gccggcaagc atgatgatgg
     6841 gaatgccctt tcttgccttc ttgtccgtag ttgcagttgg attttttgca tcccgagtgg
     6901 cggcttctta tactagtagg ctgagaagtg atatttttaa ccgagttttg gattactcgc
     6961 agacagagat taagaaattt tcaattccta gcctcttgat gcgtactacc aatgacatta
     7021 ctcaagttca aatgttgatt actatggact tgcaagtggt aacgtgtggt ccaattatgg
     7081 ctatctgggc tattgggaag attttaggtc attcagaata ctggctctgg gccgtacttg
     7141 tggcagtgat tgtcaacgtc ctgatgacga ccgttttgat gacgctagcc tttccaaaac
     7201 agtccttgat tcaggggctg acagataaac tgaacagtat cactcgtgag agtttaacag
     7261 gtattcgtgt cgttcgtgcc tacaatgcag aggattatca aaatgaaaaa tttgcagcag
     7321 taaatgatga attgacccgt taaaatttgt ttgtcaaccg tcttatggct attttgaatc
     7381 ctatcatgat ggggatttca agtggtttga gtgtggcgat ttactggatt ggggcctatg
     7441 tgattaacga cgctgctccg atagcgcgtc tgcctctctt tagtgacatg attgttttca
     7501 tgtcttatgc catgtaggtt gtcatgggct tccttctcat gggagcactc ttcatcgttc
     7561 ttccccgaac tatggtctct gctaagcgga ttaatcaagt tttagatttg cattcttcta
     7621 tccaaaaccc tgttcaagtg cagctgactg atgaaaactt caaaggtcag gtcgagttta
     7681 aggatgtgac cttccgctat gcggcaaatt cggaggcagt tattgaacat gttagcttta
     7741 aagcagaaac tggtcaaaca gtggccttta ttgggtcaac aggttctggt aaatcaactc
     7801 tggtcaatct gattccacgt ttctacgacg tgtcagcagg agaaattctg gtggacggtg
     7861 tcaatgttca agactatgac ttctctgcga cagctcatgc tggtcaaaag gttgccattg
     7921 ttgggccgac tggggctggt aagacaacca ttgtcaatct tttgatgaaa ttctatgaga
     7981 ttgataaggg aagtattcgc attgatggtg tggataccaa ggctatgacg cgttcagaag
     8041 tgcatgatgc cttttcaatg gtcttgcagg atacctggct ctttgaagga actattcgag
     8101 acaatctcat ctataatcaa atagggatta gtgatgaacg aatgatggaa gctagtaagg
     8161 ctgtgggaat tcaccacttt attatgacct tgccagatgg ctatgatacc atcttggatg
     8221 acaccgtgac cttgtctgta agacaaaaac aactattgac tattgctcgt gcccttctta
     8281 aggatgcacc gcttttgatt ttggatgagg cgacttcttc tgttgacaca cggacagagg
     8341 aattgatcca aaaagccatg gaccgtttga tggaaggacg cacatccttt gtcattgccc
     8401 accgcttgtc aaccatccga aatgcagact tgatcttggt catgaaagat ggaaatatca
     8461 tcgagcaagg caactatgag gaactgatgg cgcaaggtgg cttctacgct gacttgtaca
     8521 atagtcaatt tacagaagat gaagcagaag aataaatcaa aagaaggctt gacgaccttc
     8581 tttttcttat ttatactagt acacatagaa agagagaagt atgagtcaga aattatacaa
     8641 tatgaaattt gctgctgtct acttagcctt gattgcaaaa gttgagcgaa agggtggaaa
     8701 ggcagagtca gttcaccaag tgaccagttg gttgacaggg tatgaagtga gtgacgttct
     8761 tgcttgtctg gatagggatg ttacttacgg agattttttt cgacaagcac catattatgt
     8821 ccctgaacga attgcaatta cagggaagat ttgcggtgtc cgtattgagg aaattgatga
     8881 tcctctgatg caggaaatac gtcgcttgga caagctagtg gattggcttg ccaaggggaa
     8941 aacatctcaa caagttctag aaaagtatga gaagcataaa tgaaaaatta tcaagaatgg
     9001 tatcgaaata tcagatccag actaactagt cgtccaattc ttttattcct attacgcagt
     9061 ttcaatcgtt tgatgacagt cgccatgccc ctggtctatc tgatcttgct agtcaccact
     9121 tacctgcaac taggacttgg tcagcaagtt ggggtttatg tgcttattcc tgcatcaggt
     9181 tttgtggcct tgtccctgtt tcgtaagaaa atcaatcacc cgagacctta tgaaacttgg
     9241 aatatctgtc cccttcttga taaggatagt tcgggacggt cgatgcccag tcgccatgtc
     9301 ttttcggcaa ctatcatctc catggcctgt ctgcatgcta gtctacctat tggcttagta
     9361 tgcttgatct tttcagcttt gctggggttg gtgagggttt tagggggtgt tcattatccc
     9421 aaggatgtct tggttggcta tgcttgtggt ttggtgtggg gattcctttt cttcctattc
     9481 tgacatgtaa gttaagctag tcctacaacc acttactgct tcaaattgac cacttgcttt
     9541 tttgcccttg tattcctaac ttagctttga tatagtagag tcagaaagga gatgtgatga
     9601 agttacgaat tgagattgac ggcaatttag aggagactga aattgtcatc aagaccccca
     9661 ctttgacaga tgaaattgca gacttgcaac ggctcttgca agagtcaaag gctccgaggt
     9721 tgacttttta caaggggaca ggtgaatatt atctagacct gtcagaaatt ctcttctttg
     9781 aaacagaagg gagcaagatc tacgctcata accagaagga agcttatgag gttcgcctca
     9841 agctctatga gttggaggct atccttcctc gctattttag tcgagtatcc aagtcaacga
     9901 tcgttaatat tcggcaaatc tattcggtgg acaagtcctt ttcaggaacg ggcaccattt
     9961 ccttttatca aacccacaag gaggttcatg tgtcacggca ttaccaatcc ctcctaaaag
    10021 aaaatctaag aaatatgagg taagaacatg aaaaagaaag catttggtat tgtgttgctt
    10081 gtactagcag ctttaatatt agtacaagga aattttggaa tcccttctct aggagggcac
    10141 atttggccct tgattggtat tgcttttttt gcctaccaat cagttggagc tttgttgcgt
    10201 cgccacttaa cttcagcctc ttttacagct ctagtagcct taatgattgc taaccacttt
    10261 tatggcattt taccgattcc aaaccagaca ctaatctggg caggagttct aatcgttctc
    10321 ggagtaaata tgttgactca ttctaacaga acttggaatg ggaaaaaatg gtggtatgat
    10381 ggtgaaaaaa acattcttat agataaggaa gttgcttttg ggacagggac tttctacaag
    10441 caagaccaag aattggtaga agaccaagta gaagttggtt ttggcaatgc aaaaatctac
    10501 tatgacaatg cagagatgtt aggtgatttt gcaactttaa atattgaagt agcttttggt
    10561 aatgcaactg tctatgttcc gcaacactgg cgtgtcgatt tgaaagtaga aacatccttt
    10621 ggtgcagcca aggcggacgc tcctgtagcc ccaaccagca aaaccttgat tatccgtgga
    10681 gatgtggctt ttgggaagtt ggaaattgtc tacgctaaat aaaaaaatct tcacttcaac
    10741 catcaaaata ggcgtactaa gagtaggaaa ttgatgcctt gctctgattt cagttctatg
    10801 gttgttagac tttaaaaaat gaaatgctgc ctttaaaagt tgtatatttt tcgatatttt
    10861 ggcttttacg tttgatgtat ctatgtacta cagcgtagat gatgtagtgt cgaatgcttt
    10921 taaaaaacga atgatattgg acagtttttt tgcctttaat tgctcaggaa ccatgaaagt
    10981 cagtacttgg gtttatgaca agggagaatg gtattatgta agttcttctg gttccatgat
    11041 tgcgaatgat tgggtcaaag acaatggcaa gtagtattat ctggcttcat caggtaagat
    11101 gcttcgcaat acctacacac ctgatggtta ctacgttggc aacttaggtg cctggcaata
    11161 agaataatac tctttgaaaa tctcttcaaa ccacgtcaac gtcgccttat ctacaacctc
    11221 aaaaccgtgt tttgagcagc ctgcggctag ctttctagtt tgctcttagt ataagaagtc
    11281 cttttcctta aaggaagagg ctttttactt gcttttctgc tgcttcctca tttgtcctga
    11341 agcctttttt gtgttaaaat gaatggtatg aaagctaaaa aaatgtggat ggcaggcttg
    11401 gctctgctag gtatcggaag ccttgctctt gctacgaaaa aagttgcaga tgaccgtaag
    11461 ctcatgaaga ctcaggaaga gttgacagag attgtgcgag accatttttc cgacatgggg
    11521 gaaattgcga ccctttatgt tcaagtttac gaaagcagtc tggagagctt ggttggtggc
    11581 gtcatttttg aggatggccg tcattatacc tttgtctatg aaaatgaaga cctagtctat
    11641 gaggaggaag tcttatgata cagccagcaa gtttagaaga attagcatct ttagtggaaa
    11701 aagcgggcaa gaaggtcttc ctttttgtgg cagactggtg tggcgattgt cgttatattt
    11761 atcctgcctt accagagatt gaagagacca atccagagtt cacctttatt cgaatggacc
    11821 gagatcagta tatggatttg gccaaactct gggatgttta cggaatttct agccttgttg
    11881 ttctagaaaa ggacaaggaa atcggtcgtt ttgtcaatcg cgactgtaaa agtaaggagc
    11941 aaattaacga ttttttagca ggattgaaat aggagaaaaa ggaaacaatg atttttacgt
    12001 ataacaaaga acatgtcggt gatgtcctta tggtcatcgt gaaaaatagc ggagatgcca
    12061 aactgaatgt ggaacgcaaa ggcaaggtag cccgtgtttt cctcaaagca aatggggaaa
    12121 cagtagcttg gaacattttt gaggtttcga gcttgtttga aattgcagag cgcggtcaag
    12181 tctttttatc agatgagcaa gtcgctcgtt tgaaccaaga attacaggcg gaaggtttta
    12241 cagaagaaat tgttaatgat aaggaaccta agtttgttgt tggtgaaatt gtcgagatgg
    12301 tagctcatcc agacagtgac cacctcaaca tctgccaagt tgcagtcgca agtgacaaga
    12361 tagtgcaaat cgttgcagga gcacctaatg cgcgtgttgg gttgaaaacc attgtggctc
    12421 ttcctggtgc catgatgcca aaagggaatt tcattttccc aggcgaactt cgtggtgaaa
    12481 agagttttgg gatgatgtgt agtcctcgtg aattgcattt gccaaatgct ccgcaaaaac
    12541 gtggggtgct tgaattatca gaagaccaag ttgtcggaac accgttcgac ccagcgaaac
    12601 actggactgc ctaggaactt gtcagcatct gatagaagga aataagatgg caaaaaatgt
    12661 agtgattacg ggagcaacct caggaatcgg tgaagcgatt gcgcgtgctt atctggagca
    12721 gggtgaggat gtcgttctaa caggacgacg gatagacaga ttagaaatcc tcaagtcgga
    12781 gtttgcagta agctttccaa atcaaaccgt ctggactttt ccactagatg tgacggatat
    12841 ggtcatggtg aagactgttt gctctgatat tctagaaacg atagggagga ttgatatctt
    12901 ggtcaacaac gccggactgg ctcttgactt ggctccctat caagactatg aggagttgga
    12961 tatgttgacc atgttggata ccaatgttaa aggtctgatg gcggttactc gctgtttctt
    13021 gccagcaatg ataaaagtca atcaaggtca tattatcaat atggggtcaa ccgcaggaat
    13081 ctacgcctat gctggtgccg ctgtttactc agctaccaag gctgcggtta agaccttttc
    13141 ggatggactg cgaattgata ccatcgcaac ggatatcaag gtgacaacca ttcagcctgg
    13201 gattgtcgaa acagatttct caactgttcg ttttcatggt gataaagagc gagctgcgtc
    13261 cgtttaccaa ggaatagaag ccttgcaagc tcaggatatt gcagacacag tagtctatgt
    13321 gaccagtcag cctcgccgtg ttcagattac agatatgacc attatggcca atcaacaggc
    13381 gacaggtttc atgattcata aaaaataaga aatttcctcg aaaagttaca aatttctgta
    13441 actttttttg atttcctacg aatagataag taggaggaag aaaatatgta taataaagtt
    13501 atcatgattg ggcgtttaac gtctacacca gaattgcaca aaaccaacaa tgacaagtcg
    13561 gtagcgcgag caactatcgc tgtgaaccgt cgttacaaag accaaaacgg tgaacgtgaa
    13621 gctgattttg tcaatatggt cctatggggc agactagcag aaactttggc aagctacgca
    13681 accaaaggta gtctcatttc cgttgatgga gaattgcgta cccgtcgctt tgagaaaaat
    13741 ggccaaatga actacgtgac cgaagtactt gtaacaggat tccaactctt ggaaagtcgt
    13801 gctcaacgtg ccatgcgtga aaataatgca ggccaagatt tggcagattt agtcttggaa
    13861 gaggaagaat tgccatttta atactcttcg aaaatctctt caaaccacgt tagctttatc
    13921 cacaacatca aagcaatgct ttgagcagcc tgcggctagc ttcctagttt gctctttgat
    13981 tttcattgag tataaacatt aaaaagtctg agttggtctc aggcttttta tcttgagaaa
    14041 gtcagacttt tttcttgatt atttctgact aagtgataca atagaactat gaattagcac
    14101 tcaggtataa agagtgctaa taatatctat ctcattatgg aggaaatcag atgttgaaac
    14161 cattagggga ccgtgtggtc ttaaaaatag aagaaaaaga acaaaccgtt gggggctttg
    14221 ttctcgcagg ttcagcccaa gaaaaaacca aaacagccca agttgtggct actggacaag
    14281 gtgttcgtac cttgaacggt gacttggttg ctccaagtgt taaaactgga gaccgtgtct
    14341 tagttgaagc ccacgcaggt cttgatgtca aagatggcga tgaaaagtac atcatcgtag
    14401 gcgaagctaa catcttggca atcattgaag aatagaagga gaaagtaagt atgtcaaaag
    14461 aaattaaatt ttcatcagat gcccgttcag ctatggtccg tggtgtcgat atccttgcag
    14521 acactgttaa agtaaccttg ggaccaaaag gtcgcaatgt cgttcttgaa aaatcatttg
    14581 gctcaccatt gattaccaat gacggtgtga ctattgccaa agaaatcgaa ttggaagacc
    14641 attttgaaaa tatgggtgct aagttagtat cagaagtagc ttctaaaacc aatgatatcg
    14701 caggtgacgg aactacgact gcaacagtct tgacccaagc tatcgtccgt gaaggaatca
    14761 aaaacgtcac agcaggtgca aatccaatcg gtattcgtcg tgggattgaa acagcagttg
    14821 ccgcagcagt tgaagctttg aaaaacaacg ccatccctgt tgccaataaa gaagctatcg
    14881 ctcaagttgc agccgtatct tctcgttctg aaaaagttgg tgagtacatc tctgaagcaa
    14941 tggaaaaagt tggcaaagac ggtgtcatca ccatcgaaga gtcacgtggt atggaaacag
    15001 agcttgaagt cgtagaagga atgcagtttg accgtggtta cctttcacag tacatggtga
    15061 cagatagcga aaaaatggtg gctgaccttg aaaatccgta cattttgatt acagacaaga
    15121 aaatttccaa tatccaagaa atcttgccac ttttggaaag cattctccaa agcaatcgtc
    15181 cactcttgat tattgcggat gatgtggatg gtgaggctct tccaactctt gttttgaaca
    15241 agattcgtgg aaccttcaac gtagtagcag tcaaggcacc tggttttggt gaccgtcgca
    15301 aagccatgct tgaagatatc gccatcttaa caggcggaac agttatcaca gaagaccttg
    15361 gtcttgagtt gaaagatgcg acaattgaag ctcttggtca agcagcgaga gtgaccgtgg
    15421 acaaagatag cacggttatt gtagaaggtg caggaaatcc tgaagcgatt tctcaccgtg
    15481 ttgcggttat caagtctcaa atcgaaacta caacttctga atttgaccgt gaaaaattgc
    15541 aagaacgctt ggccaaattg tcaggtggtg tagcggttat taaggtcgga gccgcaactg
    15601 aaactgagtt gaaagaaatg aaactccgca ttgaagatgc cctcaacgct actcgtgcag
    15661 ctgttgaaga aggtattgtt gcaggtggtg gaacagctct tgccaatgtg attccagctg
    15721 ttgctacctt ggaattgaca ggagatgaag taacaggacg taatattgtt ctccgtgctt
    15781 tggaagaacc tgttcgtcaa attgctcaca atgcaggatt tgaaggatct atcgttatcg
    15841 atcgtttgaa aaatgctgag cttggtatag gattcaacgc agcaactggc gagtgggtta
    15901 acatgattga tcaaggtatc attgatccag ttaaagtgag tcgttcagcc ctacaaaatg
    15961 cagcatctgt agccagcttg attttgacaa cagaagcagt cgtagccaat aaaccagaac
    16021 cagtagcccc agctccagca atggatccaa gcatgatggg cgggatgatg taagcattct
    16081 atagaaaaca acttataaaa aacacaaaag gagggaatga ctaacccttc tttttataat
    16141 attcacttct aaattgattt gagctctcct aacttatatg ataaaataag actagaaaaa
    16201 ggagaagaac atgatcgatg tagaaaaaat tctgtgcaag atgcccccca atcagaagat
    16261 taattatgac cgtgtcatgc agaaaatggt agaagtatgg tagaaaaatg agcaacggcc
    16321 aaccattctc gtgcatgttt gctgtgcccc ttgtagtacc tatacactag aatatttgac
    16381 caagtatgca gatgtgacca tctattttgc caattctaat atccatccca aggtagaata
    16441 ccataagcgg gtctatgtca ccaagaaatt tgttagtgat tttaatgagc ggacaggaaa
    16501 tacggttcag tacctagaag ctccctacga acccaattaa taccgaaaac tagttagggg
    16561 actagaggag gagcccgaag gtggcgaccg ttgcaaggtt tgttttgact accgactgga
    16621 taaaacagcg caagtggcta tggacttggg ctttgactac tttggttcag ccttgaccat
    16681 cagtcctcat aagaattctc aaaccatcaa cagcatcgga atcgatgtgc aaaaaattta
    16741 cacgacccac tatcttccca acgatttcaa gaaaaatcaa ggctacaaac gttcagtaga
    16801 gatgtgtgag gagtatgata tctatcgtca atgttattgt ggctgcgtct atgcagccca
    16861 agcccagaat attgacctgg ttcaagttaa gaaggacgct acagcattct tgttgggtaa
    16921 ggatgttgag aaagactatt ctcatatcaa atttatagta gattgaaact agaatagtac
    16981 acctttactt ctaaaacatt gttagaaatc gatttgactg tccttatttc attttactat
    17041 actattacga aattagatat ataaatgaaa acttgcctca aggtaggttt ttttgctagt
    17101 agaaaagcga tagacagaaa ttgttggact cttaaaaaaa tgaagttttg ctcaaaagag
    17161 cttcttaaat taaaacgagc aattatcagt tcaaatctgt ttcaaaagaa gaacggataa
    17221 attaatcgat gctttgtttt tgagttagga agttaagcat gccaaactga gtcgtaggaa
    17281 taaatttctt tatagaaatg tgttacaact ttgctataac ggaatattcc tttttgcatt
    17341 tatacaactt atttaacact atctttattc ttgtgatata atgccaaatt atagagtgga
    17401 ggtcatttaa aatgattgat aaagtaattg aacaatataa aagtcatgat aatcttgata
    17461 ttcgagtaga attgcataag aagtattcaa aaaataaatt gggatttaat aattggatat
    17521 tttctaacta ccaaatcact gatgaagtaa aggttctaga attaggatgt ggtacaggag
    17581 aactgtggaa aagcaatagt gattctatag ataaaatgaa gcagttgatt gttacagatt
    17641 tttctaaaga tatggtaaaa tcaacgaagt cagtgattgg aaatagagac aatgtcaact
    17701 atgagataat ggatattcaa aaaatttctt ttgagaatga aacttttgat attgttattg
    17761 ctaatatgct tctacatcat gtgaatgata ttcctaaagc tctctctgaa gtgaataggg
    17821 tcttaaaaac cgaaggcatt ttttactgtg ctacatttgg tgaaaatgga gttgtaaatt
    17881 atttggcaag tttatttaaa gatgaggtta atcaagattt agaaaataga acgtttactt
    17941 tacaaaatgg taaaaggtac ctgagtaggt gttttaactc tgtcgataca ttactctatg
    18001 atgatgagtt acaggtaact agtatagatg acctagttaa atacatccaa tcgtttaaag
    18061 gaatttcaga aataggttca ctagaagagg aaataatacg taaaagattg gaaagtgagt
    18121 ttaataatag tatgtcgatc attcctaaag aatatggtat gtttatcgct cgaaaagaaa
    18181 gttaagggaa cgaaagtgtg aaaaattatg gagaagcttt tcgatatttt agaaaattaa
    18241 acggatattc tttagagtat gctgcagttg attctatatc aaaatctcaa ctttctagat
    18301 ttgaaagggg agaaaatgaa atttctcttt caacattttt tgaattatta tcaaatatca
    18361 atgtttcgat agagaatttt tgtaatcact tagaacatta taaaagatcg gagcgtgatg
    18421 attttttg
//
LOCUS       DAVDWT010000038        17868 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVDWT010000038 DAVDWT010000000
VERSION     DAVDWT010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17868
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.038"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(369..2801)
                     /locus_tag="VUW50_001705"
     CDS             complement(369..2801)
                     /locus_tag="VUW50_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET7983566.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQILFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLAHQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGNTLKI
                     GVKAGQLKFDIA"
     gene            complement(2803..3261)
                     /locus_tag="VUW50_001706"
     CDS             complement(2803..3261)
                     /locus_tag="VUW50_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HET7983567.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            complement(3380..4108)
                     /locus_tag="VUW50_001707"
     CDS             complement(3380..4108)
                     /locus_tag="VUW50_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7983568.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            complement(4108..5115)
                     /locus_tag="VUW50_001708"
     CDS             complement(4108..5115)
                     /locus_tag="VUW50_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7983569.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            complement(5154..5912)
                     /locus_tag="VUW50_001709"
     CDS             complement(5154..5912)
                     /locus_tag="VUW50_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7983570.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            complement(5875..6165)
                     /locus_tag="VUW50_001710"
     CDS             complement(5875..6165)
                     /locus_tag="VUW50_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HET7983571.1"
                     /translation="MKASIALQVLPLVQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMCILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     regulatory      complement(6235..6337)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(6420..7766)
                     /gene="cbpD"
                     /locus_tag="VUW50_001711"
     CDS             complement(6420..7766)
                     /gene="cbpD"
                     /locus_tag="VUW50_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HET7983572.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSASTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     gene            complement(7855..8121)
                     /locus_tag="VUW50_001712"
     CDS             complement(7855..8121)
                     /locus_tag="VUW50_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983573.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            complement(8123..9475)
                     /gene="dnaB"
                     /locus_tag="VUW50_001713"
     CDS             complement(8123..9475)
                     /gene="dnaB"
                     /locus_tag="VUW50_001713"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET7983574.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEILAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            complement(9519..9971)
                     /gene="rplI"
                     /locus_tag="VUW50_001714"
     CDS             complement(9519..9971)
                     /gene="rplI"
                     /locus_tag="VUW50_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET7983575.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            complement(9968..11941)
                     /locus_tag="VUW50_001715"
     CDS             complement(9968..11941)
                     /locus_tag="VUW50_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET7983576.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDHVFVVGHKNLDMDALGSSVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            complement(12077..12625)
                     /gene="raiA"
                     /locus_tag="VUW50_001716"
     CDS             complement(12077..12625)
                     /gene="raiA"
                     /locus_tag="VUW50_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET7983577.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            complement(12705..13367)
                     /locus_tag="VUW50_001717"
     CDS             complement(12705..13367)
                     /locus_tag="VUW50_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET7983578.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            complement(13364..14662)
                     /locus_tag="VUW50_001718"
     CDS             complement(13364..14662)
                     /locus_tag="VUW50_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7983579.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQVVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLDLYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            14718..15353
                     /locus_tag="VUW50_001719"
     CDS             14718..15353
                     /locus_tag="VUW50_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HET7983580.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            15638..16558
                     /gene="cysK"
                     /locus_tag="VUW50_001720"
     CDS             15638..16558
                     /gene="cysK"
                     /locus_tag="VUW50_001720"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET7983581.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(16755..17153)
                     /locus_tag="VUW50_001721"
     CDS             complement(16755..17153)
                     /locus_tag="VUW50_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7983582.1"
                     /translation="MKAPIPKAPLAHSLGSASIIAHTIHQKFNLKVPNYRQEEDWAKM
                     GLPITRKEIANWHIKASQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            17144..17452
                     /locus_tag="VUW50_001722"
                     /pseudo
     CDS             17144..17452
                     /locus_tag="VUW50_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(17593..17868)
                     /gene="tnpB"
                     /locus_tag="VUW50_001723"
                     /pseudo
     CDS             complement(17593..17868)
                     /gene="tnpB"
                     /locus_tag="VUW50_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT         5314 a         4072 c         3057 g         5425 t
ORIGIN      
        1 tattagtttg atttttcaaa taccagctag aacggatttg acatgcaaaa aagaaacagg
       61 tctcttttga aagaataaaa atttcagata agactgctct ttttctacta cacacgctct
      121 aagacaaata cgacggaggg taataaacat atgaattttg tgttgaatgg tgaaaaccac
      181 ggatcgttta gaagctatag gttttagttt catactcttc gaaaatctct tcaaaccacg
      241 tcagctctat ctgcaacctc aaaacagtgt tttgagcaac ctgcagctag ctgcctagtt
      301 tgctctttga ttttcattga gtttcactcc actgtgactt atggtcagat gctgatactt
      361 ttattctttt atgcaatatc aaattttaac tggcctgctt tgacaccaat cttaagtgtg
      421 ttgcctgcca ctaaatctcc cttaagaaga agttctgcca acttgtcctc cacttctgtt
      481 tgcagggttc tgcgaagtgg gcgagctccc atctctgggt catatccttg atgtgctaac
      541 aatttcagag ctgaagcttg taatttcaag tcaatgcctt tttcagccaa acttgccact
      601 aaaggcttga ccataatctt caccacttcc tgcatatggt cactagatag gctatggaag
      661 accaccttct catcaatacg gttgatgaat tccggtctat aagctttttt cagttcttca
      721 aacatgcgtt tttccatatt ttcctggtca aaacgaatat ccttagcccc aaaaccaaca
      781 gtcttatcat cacgaagggc agtcgcacct agattcgatg tcataatgat aatggtattt
      841 gaaaaatcga ccttgcgtcc cttgctatct gtcaagacac cgtcatccag aacctgcaag
      901 agaacattaa agatatctgg gtgggccttc tctacctcat caaagaggag aacggaatag
      961 ggtttattgc gaaccttctc tgtcaactcc ccaccttctt cgtaacccac atagcctgga
     1021 ggagctccat ttagacggct ggctgcgaat ttctccatat actcactcat atcaaagcgg
     1081 ataagggctg attcgtcatc aaagagaact tctgccaaag ccttggccaa ctcggtctta
     1141 ccgacacctg tgggtcctag gaacataaag gaaccgatcg gacgcttgtg actgcgaatt
     1201 cctgactgat tacggcgaat ggctcgacta atacttgaaa cagcttgatc ttgaccgatg
     1261 acacgtttat ggagttctgc ttcaagattt aaatacttct tagcatccgt ttgagtcagt
     1321 ttttgaactg ggattcctga caagcgactc aaggtggtca aaatatcaga ctctgtcacc
     1381 aagtctttgt agacaggtac ttcctcttct tttgcgatta gctgggctgc ctgtttccac
     1441 ttgccatcca tcagggcctt gtcagctgga ctcaaatctg aatcgtctgc ttttacatgc
     1501 tttgccttat tttgcactgt tgctgctgct tcgtccaaga ggtcgatagc agagtctggc
     1561 aagtgacgac tggttaaata acgatgagcc atcttaaccg ctgtttcaac cgcttcatct
     1621 gtgatttgta cacggtgatg tttctcataa gtcgccttca aaccttgtaa aatagtcata
     1681 ctatctgcca cacttggttc ttcaatcgtc actttagcga aacgacgaga aagtgccgca
     1741 tctttttcga tatgtttttg atattcttcc tgagtggtgg caccaaccgt tctcaaagtt
     1801 ccacgcgcca aggctggttt caagatattg gccgcatcca gagtcgaatc aatcccgcta
     1861 ccagaaccca tgatggtgtg gagttcatcg ataaagagga tgacttggcc atcttcttca
     1921 atatccttga tgatattatt catgcgttct tcaaagtcac cacggaagcg tgtccctgca
     1981 acgacattca tcaaatcaag ttctaacacg cgcatcttag ccatttccgc aggcacgtca
     2041 ccactggcaa tacgctgagc aagaccaagc gccagagctg ttttcccgac accagcatcc
     2101 ccaaccaaga cagggttgtt cttagtcttc cggcttaaga tttgaatcat acgtgagatt
     2161 tccttgtccc gaccgatgac tggttctaac ttgccagaac gcgcttgctc tgtcaaatca
     2221 tgcgtatagt cctcgagacc accactagga gtctgcggca tgcccatcat attggccata
     2281 gaattttgct tgtcagctac tgtacgatgg cgttggcgta aagccttgag atcttcacga
     2341 gtccagcctg cccgttcttc taaatttcga cgaagagcag caatcttgac ctgatctttc
     2401 ttgtcttcat aagaaaaacc agccctctcc aagatacgag tcgccaaggc attgccatca
     2461 tgcaaaatcg catagaggac gtgctctgta cctagcacct tagcatggac cactgacgct
     2521 acatactctg cttcatcaaa aagaatctgc aaacgacggg agaacggcaa ttccgtaaaa
     2581 gtttcatcct ggctatagtc cgtttcagtc agttccaaag ccacctcttc taaacggtcc
     2641 atctcatacg gataatcatt taaagttgcc cctgctacac tataactgtg attagacatg
     2701 gcaatcaaca agtgccaaga ctctagataa cgagctccaa aatgtccagc aaccatgtag
     2761 gcactttcga tacattcatt caatgctttt gaatagttca tcttacttcc cttttctatc
     2821 tacctcttgt atgatctgac gtagcatgtt tgctcgaaca actggagctt cttctcctaa
     2881 aacgcgatcc aaagctactg attctagcaa attcatctcc tgcttggtca tcaattcctg
     2941 ctcaaccaaa agctggagaa tatcctcata aatttcttga ctgactcgct caccaatcga
     3001 gtaaagcagc tcccggagca tttcatgatg actagaaaac tcaatccgtc ctatacgaat
     3061 gtagcctccg ccaccacgct tactttcaac caagtagcct ctactttccg taaagcgtgt
     3121 cttgatcacg tagttaatct gactaggaac aacctgaaag gtatctgcca actgactccg
     3181 ttgcaactcc acgataccag attgatctaa aatcgccttg atgtaggcct caatatgatc
     3241 cgatgtattt ttaaatctca taacaaaccc acctctctct ttaaaccttg accttgtttg
     3301 actattatac cgaaattttc tcatttttaa aagaaaaagg gcgctggtaa aggataatct
     3361 tcaccaactc cctatttttc tacttatcta agcctaattc tgccaagatt tgacgtttat
     3421 aggcaatctt ttggacttcc ttgtcctcat cttcagacca atctagttta atttctgaaa
     3481 caatctgccc agggcgattt ttcaagatat agatgcggtc gctgagattg agggcctcct
     3541 caatactatg cgtgatgatc agggttgtta gctgcaactg cttgtgaatc tcaagatacc
     3601 aagcgtggag ttccatcttt gtcatctcat ccaaggcgct aaaggcctca tctaagagaa
     3661 agagcttgtg cccaaaaagg taggtccgga gtaaggctac acgctggcgc atcccaccgc
     3721 taagttcatg aggatacttg tctcttacag ctgtcagctg gaaggtcgca agaattttat
     3781 ccgctcggga aatagcttct gccttatcca ccttttgaat caagaggggc agaatgatat
     3841 ttccaagcac cgtcttgtgc tccaagagca gatccttttg caacatataa ctcacgcgcc
     3901 ccttgggatt ttcttcacca tcaaggacaa ttctccctga ctgaacttct aaaatcccag
     3961 cgattagatt aaagagggtg gtctttccaa caccacttgg gcctaggatg gaaaccactt
     4021 cgcctgaagt cacctgtagg ttgatatcct ctaaaatcct ctcctgacca taggcataac
     4081 tgacgtgctc tagtctaatt tctgtcatta tttcacaaat tcgttggtga agcctttgtc
     4141 tgtcaagtct tctttaagga taccattttc tttatcccat ttgtagaaag cattccagcg
     4201 agctgcgtca aattgacccc acttttcctt gtcgcttgcg tattcttttg acaagtattt
     4261 ttgagattcg atgacaaagt cacgtttttc cttgagttca ggtgcattct tgatgagaat
     4321 atctgcagct tcttctggat gttccatggc atattggtag ccttttttga tggcttggat
     4381 gactttgcga gcttcttctt tgttatcttt cagatagtcg ttgtttgcga tgataactgg
     4441 tgaatagtag tcaaactcct tgacatagtc tttcaagtac atgaagttag catctacacc
     4501 ttgagattta gcaaggatac catcccaacc gtagtaaatc caagcagtat caaagacgcc
     4561 attggcaatc ggtgtgattg agtttgagtc gttatttggt actttttcaa ccttctcaaa
     4621 gtctccacct tgagattcta ccaaggtttt caacatagca agttcagttg ggtcattcca
     4681 tgtcccatat ttcttaccaa ccaagtcttt tggactgctt acattatcag atttacgaga
     4741 gatgattcct gatgtattgt gttcaacaat agctgcaacg gcagtgattc ctgctccttt
     4801 ttccaatttc ttagccatgt agtcttggaa atacactgca aatggtgcct ttccgttgat
     4861 aaccaagtca gaagaacttt cttctggtgg caatttcaaa tcaacatcca ctccagcttc
     4921 tttgaaataa cctttttcct tggcaacata aagccctgtg tggttggtat ttggtgtcca
     4981 gtctaggata aagtcaacct tcttaagttc tgcctctttg ttgtctttag aagcagttcc
     5041 ttgaccacag gccacaagca caacagctac aagagctgtt acaagcgtta aaaacacttt
     5101 ccatgttttc ttcattttga tttcctcttt tctttttcag aaacattcta attctacgaa
     5161 cgtttccatt taatcacata tttttcactg atatcgacca gcttcatacc caaaagactg
     5221 ataatcgaca ccagaataat aatggcaaac atggtatcat actgaaacag ttttttagac
     5281 tgaatcatat aaacaccaag accttcaaaa cctcccaacc actcagatac cacagttgtg
     5341 ataaaggcgt aggagacact gaccctcaga cctgcataaa agtaaggcag gctaaccggg
     5401 attttaaaat gccacaggat ttgccaaggc ttggctcgca tcagactaaa caaggtcagc
     5461 atatccttgt cacaatgcct aaaaccgtcc aaaatactaa cgatgatggg aaaggttgtc
     5521 gttaagataa tcaagacaat cttgggcaaa atcccataac ctagccacaa gaccaggata
     5581 ggagctatgg caatggtcgg aatggtctga atgaccacca tcatagggta aatcaggtca
     5641 ttgagccaag tcaaactatc catgagcaca gccataagac aggcaatcaa aactcccaaa
     5701 atcagcccca gtaaagccac tctcaaggtc gcccagctat ggtgccagag aaattctctg
     5761 tcacgaacaa agggctggag aatttcaaga ggtgtcggca ggataaactt ggggagaagt
     5821 ttaagaaaac ctgctaactg ccagattgac aatactccga gaaagcccaa tagactaatg
     5881 tgtcgtctca gtatacttct caagtttctc atcaatactt aaaatctctc ctacatttat
     5941 tttgacattg gcaaagacat tgtctgcctc ctgccctgcc acttccagcg cttcttttag
     6001 aatgcacata agctcatcaa actccccttc caagaccgtt tcaaatggtg tcactaccat
     6061 cgtcacttct tgagtttgca gataagcaat gacctgatca ataacagcta tccgatcaat
     6121 cccctgtact aggggtaaaa cttgcaaggc aatgcttgct ttcataagaa cctcctcttc
     6181 tcgttttcct ttttaaaaaa gaaaaacctt taccatacat ggaaaaggtg caaaattaga
     6241 ctaagtatcg ttccctccgc tggcattacc cagatcaggt gcggtcgaag tttaacactt
     6301 cctctcagac tgtacacaga ctcccatata ttatatgtat atatgatagc gcaaaataag
     6361 aaaaatgtca aggaaactgc ttacagaaaa gagcaggaaa tttctcctac tccaattttc
     6421 tatactcgtt ctccatcact gtttactctg tagccatcca ccgtcgtatt aacagctaag
     6481 gcgcctgaag agtaagaata ataccactta ccagagacct ggtaccagcc tgttttcatt
     6541 gcccctgaac tacccagata ataccacgag ccattctcct ttaaccatcc tgtctgcatt
     6601 tcaccagata atttcaaata ataccagcta gaaccgtctt tcagccatcc tgttgattta
     6661 gaaccatttg atttgaaatg ataccaacta ccatttattt tcttccaacc gacagttgaa
     6721 ctattattgg atccatagct agagatattt cctgattgat tctgataatt ttgtgaagaa
     6781 gtcactccct ctagctgtat atagcgacga cttccattat aggcagtata actcaaccac
     6841 ttgtagccgt ctttttcaag tatctgatca taatgaacct tctccccagg ataatagtaa
     6901 tcaatcacag ttccgctaac taggggttca gttttgatag cagacttggt cttaaaataa
     6961 tgagttccac ctgttgaaga aagaacagaa ttacctagag gatttttatt cacagcctcc
     7021 aattgaacat aacgataact tccattatag gcagtataac tcaaccactt atagccgtct
     7081 ttttcaagta tctgatcata atgaaccttc tccccaggat aatagtaatc aatcacagtt
     7141 gcactaacta ggggttcagt tttaatagca gacttggtct taaaataatg agttccgcct
     7201 gttgaagctg aggattgact attcccaaca ctgccaccat ccaaatcttt aaaatgaata
     7261 aatcctgtca tcgtgtttgc ttttataact cgtttattat aggattctgt ataaccatag
     7321 ttatattcct caatctcaat ctgatctccc attacatttg acacccaggc aacatgacca
     7381 taagttcctg cagtagacca agtaatggaa ccaatcgtcg gtgtattatc tacacgataa
     7441 ccttcccgac gagcacgatg tccccattca ttcgcatttc cataagctgc cggaatttca
     7501 aaaccattga cattactcaa acgaaaggct acaaaagaag tacactgacg agaatacatg
     7561 cgccactgat caatctcctg gctcccattt ttataataag caggataatc atccccacgc
     7621 gcaatcgatc catttcctct ggaataggca tagacactat caccagctgc tagcatcaat
     7681 cctactacta aaaaaggaac aagcttttta actgacatct tcaagtaata acttgttcct
     7741 cttgctataa acggtaaaat tttcattctt cctccttgaa aaataatata aagcgatgat
     7801 tgccttcacc taaattattc ggagaaaaaa ggaaaagtga gaaaatttct cactttagtc
     7861 aagataatga atcaaattct tttctgtaag aatatctgag taagtaaagg attcaacgta
     7921 aacattaact tgtttatctc cttccacatc cccaaactcc acaataaata gggatggata
     7981 aacttcaatt agcttaccta atctattttt ttggcgctta cgaccattct ccaaagtcat
     8041 ttctacgact tgtccctcat gtgccttgat ttcttctttg atttttttca tcttggctac
     8101 atctgtaaat gcatctgaca tcttatgcct ccctctttga gatacttgaa aatttattgt
     8161 attctttttg gaaaatcaat tccactgttc cacgagctcc actacggttt ttctcgataa
     8221 taacttccac cttattattt gggataccct cctcttcttc accaccacgt tcatagtagt
     8281 catcgcgata gagaaaagct acgatatcag cgtcctgctc aatagaccca gattcacgaa
     8341 tatcagacaa gaccggtctc ttgtcctgac gttgttctac accacgagaa agctgactca
     8401 gagcgattac tggaaccttc aattccttgg ctagtatttt caactgacga gaaatttcag
     8461 aaacttcttg ttgacgattt tctcgaccag ttcccgtgat aagttgcaaa tagtctatca
     8521 aaatcaaacc aagatttcca gtttcttgag ccaatttacg agaacgagaa cgaatctctg
     8581 taatccgaat acctggcgta tcatcgatat agatactggc gttagctaga ttaccctgag
     8641 caatagtata tttttgccac tcctcatctg tcaattgccc tgtacggata gaatgtgact
     8701 ccaccaagcc ttctgcagct aacatacggt ctaccaagct ttccgcaccc atttcgagtg
     8761 aaaaaatagc aaccgttttg tccaacttag tcccaatatt ctgagcgata ttcaaggcaa
     8821 atgctgtctt accaactgct ggacgagctg ctaagataat caactcctcc tcatgaagtc
     8881 ctgttgtcat atgatccaaa tcacgataac ctgtcgcaat acctgtaata tcggtcgttt
     8941 gttgcgagcg agcttccaga tttccaaagt tgagattcaa cacatctcga atgttcttaa
     9001 acccgcttcg atttgcattt tcactgacat caatcagccc tttttctgcc tgagcaagaa
     9061 tttcatcagc tggttgtgac gcttcgtaag cttggttgac agactctgtc aacttggcaa
     9121 ttaaacgacg tagcattgct ttttctgcaa caatcttagc ataatactcc gcattagcag
     9181 aagttggcac agaattgaca atctcaacca agtaagacaa gccaccaata ttctgtaaat
     9241 caccttgatt atcaaggata gtacgaaccg ttgttgcatc tatggcatca ccacgatcgg
     9301 ataaatcgac catggcttgg aaaatcaaac gatgggcata cttaaaaaag tcccgagact
     9361 caatgtattc tcgcacaaaa acaagtttac tctcatcaat aaagatagcc cctaaaacgg
     9421 attgctcagc taagatatct tgaggttgta ctcgtaactc ttctacttct gccatcagac
     9481 ttcccttcct tttacaatct tgtcaagaag gtgtaaactt atccttcttt cacacgaaga
     9541 ttgattacac ttgtgatatc ttgatagatt ttcactggca catcaatcaa accaaccgct
     9601 cgaatcggag cttgtacttg aatatgacgt ttatcaatct taattccaaa ttgcttttgt
     9661 aattcttctg caatcttctt attggtaata gaaccaaagg tacgaccatc tggaccaact
     9721 ttttcaacaa attctacaac agtttcttct gcttcaagtt gtgctttaat tgctttccct
     9781 tctgcaatca tctcagcgtg agctttttct tccgattttt gtttaccacg aagttcacct
     9841 acagcttgag cagtcgcttc tttggctaga ttctttttga taagaaagtt ttgcgcatac
     9901 cctgttggta cttccttaat ttcgcctttt ttaccttttc ctttaacatc tgctaaaaag
     9961 attactttca ttcttctttc tccttttcct ttatttcatt taatacaatt tctgtcagtt
    10021 tttcacctgc ttctgacaag gttacatctt taatttgagc tgctgccaaa ttaaagtggc
    10081 ctccaccgcc taactcttcc ataatccgtt gtacattcag tttactacga cttcgagctg
    10141 agatagagat aaatccttgt gtattcttcg caagaacaaa actcgcttca atacctgaca
    10201 tggctaacat ggcatctgct gccttactaa taacaactgt atcatagcat ttcgtgtcct
    10261 tagcctctgc tattagtaca tctgaaccta atttacgccc ctgtaaaata agttcattga
    10321 cctcacgata ttcttcaaaa tctgtcgcag cgatttcctg gatagcaata ctatcacttc
    10381 cgcgcgttct gagatagcta gcaacatcaa atgtccgact agttactcgc gaggtgaaat
    10441 ttttagtatc caacatcata ccagccatca agacacttgc ttgcatacga ctcaaacgat
    10501 ttttcttaga attctggaac tgaatcaatt ccgttaccaa ctcactggca ctacttgcac
    10561 cactttcgat ataagtaata accgcattat ctggaaaatc ctgatccctt ctatggtggt
    10621 caataacaat ggtttgggta aataaatcat aaaattcttt tgataatgtt aaggctgtct
    10681 ttgaatggtc tacaagaatc aacaaagaac gattggtcac catccccatt gcatccttaa
    10741 cagacaacaa cttcgtaact ccttcttttt ctatgaatga aacagctcgt tcaatatctg
    10801 gagacatttg ttcttcatca taaagagcat agctattttc aatcacattg ctggcgaaca
    10861 actgcatacc tacagaagag cccaaagcat ccatgtctaa atttttgtga ccgactacaa
    10921 aaacatgatc tacactccga atcttatctg aaatagctgt catcatagcg cgcgtacgag
    10981 tccgtgtacg cttgattgaa gcagcagacc caccaccaaa ataaactgga tttttcgttt
    11041 cgtcgttttc cttaacaacc acctggtcgc caccacgtac ttcagccaag ttcaaattga
    11101 gcaaagcaac tttccctatc tcatcatgat ttccatcgcc ataagaaaat cccatactta
    11161 aggtcaaggg caactgtctc tgtttcgact cttctctgaa agcatcaata acagaaaatt
    11221 tatcattcat caagccctca agcaccgtgt agtcagtaaa tagataaaat cgatccatac
    11281 ttacccgacg agaaaacatc atgtattttt ctgaaaactc tgatataaaa ttagctacaa
    11341 aactattgat ttgactaata tctgactcag aagttgcatc ctccaaatca tcataattat
    11401 ccacagagac aatcccaatc actggtcgac ttgttaccaa ttcatctgtt atggcttgtt
    11461 ccctggatac atctacaaaa tacaaaacac cggaagaagc atccatatga acagcataac
    11521 gcttctcacc aagcttggca taagtagacg gatttcctac tgaagccttg ataatcgttt
    11581 gaacagcttc taaatcaaaa tcaccatctt ccttggtcaa aatcaattca gcatagggat
    11641 taaaccactc aacctctcca gaagataaat tcaatttcat aacacctaca ggcatttgtt
    11701 ccaatagaga tgtcaaactt tcttccgctt ggtggtttac atactgtatc tgttctacat
    11761 cactccttgt ataatgcact ctcagtttct taaataaaaa aacatagcct cctacaaaaa
    11821 gaaacaaaat taaaaccgtc aacagattat tattaacaaa aataatgaaa gtggataaga
    11881 ctccaaacgc aatcaatcct actagaatag gaaaaattgg acttacataa aattttttca
    11941 ttcaaaacct cttggcaccc attataccat aatacccctc aaaaagcgac tttttaaaag
    12001 tgtaatcagt aattctatca attataagaa aaaggtagtt tacaattcag taaacctacc
    12061 tttacacata ttgaaattaa gattctttaa cctctaacaa accaatttcg ccatcctcac
    12121 gacgataaat cacattggtt gtctgatctt caacatccac atagataaag aaatcatgcc
    12181 ccaataaatc catttgtaga attgcttctt ccaaatccat tggttttaaa tcaatttgtt
    12241 ttgaacgaac aactttagac tggacaacat ttgaatcttc caccaaagca tctgtaaata
    12301 attgaccagt tgctacctta tttttatttt tacgctcgat ttttgtttta tttttacgaa
    12361 tctgacgttc aattttatca gttacaaggt caattgaacc atacatatct tgagatacat
    12421 cttctgcgcg gagagtaata gatccaagcg gaatcgttac ttccacttta gccgtttttt
    12481 cacgataaac ttttaagtta attcgggcat ccaactcttg ttctggttgg aagtactttt
    12541 cgatcttttc gagtttagaa actacataat cacgaattgc ttctgttact tctaggtttt
    12601 caccacggat actatattta atcatatgag taccttcttt ctaaacattt ttgtttttat
    12661 gattttatta taacgctttc attctatttt tgcaaatttt ttcctcatct tacaagggaa
    12721 aatgttttta catccttagc accagcttct tccaacagtt tcttaacacg atttatagtt
    12781 gctcctgtag tatagatatc atctataagt aggatttttt taggaatagt gactccactt
    12841 ttaataaaga aaggaagttc tgtccccaag cgctctgaac gatttttaga agaactggct
    12901 ctctcttctc ttttctctaa taaatccaga tactcaaagc ctgctgcctc taccaagccc
    12961 tcaacctgat taaatcctct attagcatat ctatcaggac ttaggggaat tacaacaaat
    13021 tgatactctt tgtacttttt caactcctca cttaaaaatg aagcgaaaac ttttcttaac
    13081 aggaagtctc catcaaactt ataccgactg aaaaaatcct tcatagcttg attgtaagta
    13141 aaaatcgctc tatgactgac ttcaactccc tctttacacc aaagttgaca atcttgacac
    13201 tttgttgaca actctgtttt catacaattt ggacagttct cttccccaat tctttcaaaa
    13261 gtagaatcac agtctgaaca aagacaagag tcatcattcc tcagaagtaa gagactacta
    13321 aaagttaaaa cagtcttcat agtctgccca cataacaagc acttcataga ccagcctcct
    13381 tattcatcat ctgaatttcc ttaatcgcct tcttgattga agcatttaac ccatcatgga
    13441 agaaaagcaa atctcctgtc ggtctatcca tgcttcgtcc aactcgtcca ccaatctgaa
    13501 tcaaactaga cttggtaaac aaacgatgat tggcctctac tacgaaaaca tccacacaag
    13561 ggaaggtaac tccacgctcc aagattgtcg tactgataag tattgtcagt tctccatctc
    13621 gaaaagcttg tacttgctct aatcgatcct ctgttacaga agatacaaag ccaattttct
    13681 catttggaaa ttgctcctgt aagatttctg ctaactgctc ccctttctta atttctgaag
    13741 caaaaatgag taacggataa gctgtctttc tctgcttctc aatataggac tttaactttg
    13801 gtgacaaacg attcttgtct aagtagcgat taaaatccga taaccaaatt ggttttggaa
    13861 taatcaacgg atttccatga aaccgtctcg gtaaattcag tctttttagt tctcctaaac
    13921 ggaccttttt atctaactca ttggtcgaag tcgctgttaa aaagattctc aatccattct
    13981 cctttacact attcttgaca gcgtggtaaa gcatgggatt atcaacataa ggaaaagcat
    14041 ctacttcatc cactatcagc aaatcaaaag cttgataaaa cttcaataac tgatgggttg
    14101 ttgcaacaac tagtggtgtt cgaaaataag gttccgattc tccatgtagc aaagctatcc
    14161 cgcaagaaaa atcctgttgc aggcgcttgt acagatccaa acaaacatct atgcgaggac
    14221 tagccaaaca cactgcacca cccgcattga tcactttagc cactacttga taaatcattt
    14281 ctgtctttcc agctcctgtt accgcatgaa ctaaggttgg cttttgcttg tctactactt
    14341 gaagcaatcc ctctgacacc ttctcttgaa aaggagttaa ttggccgcgc catttgagaa
    14401 catcttgctt tggaaaatcc tcctgcggaa aatagtataa agtttgatca cttctgactc
    14461 gcttcatcag caagcactct cgacaatagt aagcaccgat gggcaaatac cattcttcta
    14521 gaatagtact attacagcgt tgacagaaaa gtttcccctt ctcctttctc attgctggaa
    14581 gtttctccgc caactgacgt tcttcttctg ttaattcatt ctcagtaaat aaacgaccga
    14641 gataatctaa atttactttc atacttcttt attcgtaaaa actagcactt tagatgattt
    14701 tttagtacaa ttaaatcatg gaatttagaa caattaaaga ggacggtcaa gtccaagaag
    14761 aaatcaaaaa atctcgcttt atctgccatg ccaagcgtgt ttatagcgaa gaagaggctc
    14821 gtgacttcat tactgccatc aaaaaagaac actacaaagc gacacataac tgctctgcct
    14881 ttattattgg agaacgtagt gaaattaaac gtacaagtga tgatggtgag cctagtggta
    14941 ctgctggtgt tcccatgctt ggggtactag aaaatcacaa tctcaccaat gtctgtgtgg
    15001 tcgtgacacg ctactttggt ggtattaaac taggcgctgg aggactaatt cgtgcttacg
    15061 ccggcagtgt cgccttagct gtcaaagaaa ttggtattat tgaaataaaa gaacaggctg
    15121 gcattgctat tcaaatgtct tatgctcagt accaagagta cagtaacttc cttaaagaac
    15181 atgatctcat ggagctggat acaaacttta cagatcaagt cgatacgatg atttatgttg
    15241 ataaagaaga aaaagaaact attaaagctg cacttgtgga gttttttaat ggaaaagtca
    15301 ctttaactga ccaaggttta cgagaggttg aagttcctgt aaacttagtg taaacaatga
    15361 ataatacagc gtttcgttga cattctcaca actactttag cgagcaaaat aaaaagatgc
    15421 gtaccaaaat atactagaaa atgaagcaat tcaaacgaaa cctgatatcg ttttccttca
    15481 cacctattta ctagaattag ctgaacgcaa tcacttgaaa attaatgact ttgatctatg
    15541 atatatagaa atcctatcgc ttcgatagga tttctatatt ttacaaatgg tatggatagc
    15601 gttatactaa agatatctta tacaaagagg tattcatatg tctatttata acaacattac
    15661 tgaattaatc ggtcaaacac cgattgttaa acttaacaac atcgtgccag aaggtgctgc
    15721 agacgtctat ataaagcttg aagcatttaa tcctggttca tctgtaaaag accgtattgc
    15781 ccttagcatg attgaaaaag ctgaacaaga tggtattctg aaacctggtt ctactattgt
    15841 tgaagcaaca agtggaaaca ccggtattgg actttcatgg gtaggtgctg ctaaagggta
    15901 taaagtcgtc atcgttatgc ctgaaactat gagtgtagaa cgacgtaaaa ttatccaagc
    15961 ttatggtgct gaactcgtcc taactcctgg tagcgaggga atgaaaggtg ctattgctaa
    16021 ggctcaagaa atcgctgctg aacgtgatgg tttccttcct cttcaatttg acaatccagc
    16081 taatccagaa gtacacgaaa gaacaacagg agctgagata ctagctgctt tcggtaaaga
    16141 tggattagat gcctttgttg ctggagtagg taccggtgga acgatttctg gtgtttctca
    16201 tgcactcaaa tcagaaaatt ctaacattca agtttttgca gtagaagcag atgaatctgc
    16261 tattctatct ggtgaaaaac ctggtcctca caaaattcaa ggtatctcag ctggatttat
    16321 tcctgataca cttgatacta aagcctatga tggtatcgtt cgtgtaacat cagatgacgc
    16381 tcttgcactc ggacgtgaaa ttggtggaaa agaaggcttc cttgtaggga tttcctcagc
    16441 tgcagctatc tacggagcca tcgaggttgc caaaaaatta ggtacaggta aaaaagtcct
    16501 tgccctagca ccagataacg gtgaacgtta tctctctaca gcactttatg aattgtaacc
    16561 atccaataac gaagtatatt gaaaaatctc cagactagag aactcacgga tagttcctaa
    16621 tctggagatt tcttatttgc acttttcttg tacaacttta gtccatggta aataggcctc
    16681 taaaacctct ttgtttacga gagtttccac gtttggaaga cattctagaa gataggatag
    16741 atatttctca ctatttataa tggattgaaa taagatatga acaaatcgat taggacatga
    16801 tggtaaagcg taatcccttg tttctcagct ttcccagaca aaaaagtcca atagtaagtc
    16861 agctgactat cactctctag caccctataa gaagtttcat ccgcatgaag taagggctga
    16921 gtcaatagtc tctctcgcaa gaggttataa aggggctcca aatagtattg actcgccttg
    16981 atatgccaat tagcaatttc cttacgtgtg attggtaaac ccatcttagc ccaatcttct
    17041 tcttggcgat aattgggtac cttcagatta aacttctgat ggatggtgtg agcgataata
    17101 gaagctgagc caaggctatg cgccaaaggg gctttaggaa taggagcttt cacaagctta
    17161 tccagatgat tatcttttac tcgttatgga caatgctata tggcataaat caagtacctt
    17221 aaagattccg actaatattg gttttacctt tattcctcca tacacaccag agatgaaccc
    17281 cattgaacaa gtgtggaaag agattcgtaa acgtggattt aagaataaag cctttcaaac
    17341 tttggaagat gtcatgaatc aactccaaga tgttatacaa ggactggaga aggaggtgat
    17401 aaagtccatc gttaatcgga gatggactag aatgcttttt gaaaacagat gagtataaaa
    17461 agaaagtcct catttcaata gaaatcacga ctttctgatg aatttatagt aaaatgaaat
    17521 aagaacagga tagtcaaatc gatttctaac aatgttttag aagcagagat gtactattct
    17581 agtttaaatc cactatattt ggggagtgat agaaaagccc ttcatcagcc aatctacttg
    17641 ttcagatgtg agagctttga catccttttc tgtactggac caagtcagtt ttccgttctc
    17701 aaagcgttta tatagtagcc aaaatccttg accatcccag taaagggctt taaagcggtc
    17761 tttacgttca ccacaaaaga gaaagacttg accggagaaa gaatccaatt caaagtgggt
    17821 tttaactaca taggctaatg agtctattcc ctacctcata tctgtctt
//
LOCUS       DAVDWT010000039        17388 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVDWT010000039 DAVDWT010000000
VERSION     DAVDWT010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17388
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.039"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            9..641
                     /locus_tag="VUW50_001724"
     CDS             9..641
                     /locus_tag="VUW50_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HET7983583.1"
                     /translation="MFHLSEWKRNEEFDFFNKMDVPYLSMSSRIEVTQAINFHKKHSI
                     SLYAIISWCVMSAINSIPELLMDTDGKIVWQYNQRGCSFTTLTSEDKLNFSSFTMGDN
                     LIEFVSAFNINKQKAEEGQKPNIDKNNIAYLSCVPWIDFLHVSTPMNLSKIDTVPRIT
                     WGKVIQENQRYFCTVNLQINHGMGDGLHVSNFFVLLQRFVNKINEYFQKK"
     gene            709..2346
                     /locus_tag="VUW50_001725"
     CDS             709..2346
                     /locus_tag="VUW50_001725"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET7983584.1"
                     /translation="MSIFIGGAWPYANGSLHIGHAAALLPGDILARYYRQKGEEVLYV
                     SGSDCNGTPISIRAKKENKSVKEIADFYHKEFKETFEKLGFTYDLYSRTDSPLHHEIV
                     QELFLQLYEKKFLYTKKIKQLYCTFDNQFLPDRFVEGKCPNCGTHSRGDQCDNCSAIL
                     DPIDLVDKRCSICSNEPEVRETEHFYYVFSEFQNLLETYLNDAEETVRWRKNAINLTK
                     RYLREGLPDRAVTRDLPNGIPVPIDGFRDKKIYVWFEAVAGYYTASVDWAQKLQNNIT
                     DFWNNRTKSYYVHGKDNIPFHTIIWPAILSGLEIEPLPEYIISSEYLTLENKKISTSN
                     NWAIWLNDIIKKYDADSIRYFLTINAPEMKDANFSWREFIYSHNSELLGSYGNFINRT
                     LKFIEKYFESEIPTKYLEGEILYNLKELYTTVGNLVESGHMKQALEEIFEYIRSANKF
                     YDDMKPWALRESDIEKCKEVLATCVIIILNLGQMLNPFIPFSGKKIEDMFKTKLNTWN
                     YISNLPNKLSDVSMLFDRIDLKKIDEEVLELQQTSSR"
     gene            2447..3214
                     /locus_tag="VUW50_001726"
     CDS             2447..3214
                     /locus_tag="VUW50_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HET7983585.1"
                     /translation="MNNLTLLKEYNFRDLGNHLTQTGQKIKPKTLFRSSKLFGISKID
                     VDLLQSYGITKVIDFRSANEIKKAPDPDIKNIKNIVIPIFYNDDSELTEFPIEFFNKS
                     DAGFQHMIKTYDQMINQKQSKLGYKKFFKLLLSHPKDESLLFHCSMGKDRTGIASLFL
                     LYILGVDMNDIFHDYLLSNKYLINVRKENIEYVNNHSGNVILMHNLLSLSSAKEEYIN
                     RVLNVLDKEYGGILRYINTELGISSQEIEELKDRYLF"
     gene            4231..5223
                     /locus_tag="VUW50_001727"
                     /pseudo
     CDS             4231..5223
                     /locus_tag="VUW50_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            5225..6042
                     /locus_tag="VUW50_001728"
                     /pseudo
     CDS             5225..6042
                     /locus_tag="VUW50_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
     gene            6044..6829
                     /locus_tag="VUW50_001729"
     CDS             6044..6829
                     /locus_tag="VUW50_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7983586.1"
                     /translation="MKKYQRMHLIFIRQYLKQIMEYKADFLVGVVGVFLTQGLNMLFL
                     NILFQHIPLLDGWSFHQVAFIYGFSLIPKGIDHLFFDNLWALGQHLIRKGEFDKYLTR
                     PISPLFHILVETFQIDALGELLVGVLLLLMTITSLTWTWAKVFLFLISIPFATLIYTS
                     LKIVTASIAFWTKQSGAIIYIFYMFNDFAKYPIAIYHSFLRWLISFIIPFAFTAYYPA
                     SYFLKDKDGLFNIGGLILISLIFFTLSLKLWNKGLDAYESAGS"
     gene            complement(7208..8403)
                     /locus_tag="VUW50_001730"
                     /pseudo
     CDS             complement(7208..8403)
                     /locus_tag="VUW50_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713014.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            8905..9108
                     /locus_tag="VUW50_001731"
     CDS             8905..9108
                     /locus_tag="VUW50_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7983587.1"
                     /translation="MYIRLKNLREDKDLSQCQIAQYLHCSQSAYSRIESGKQDIPTSF
                     LKQLAKLYQVTTDYILEMDKFSC"
     gene            9239..9391
                     /locus_tag="VUW50_001732"
     CDS             9239..9391
                     /locus_tag="VUW50_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983588.1"
                     /translation="MTTNKDNDTSERSGCQRKRTAGEMPMNDLTSHTHGGNYTCYKEE
                     KVWNNQ"
     gene            9558..11351
                     /locus_tag="VUW50_001733"
     CDS             9558..11351
                     /locus_tag="VUW50_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET7983589.1"
                     /translation="MKKNNVEIIKADSLVRRRGDNVERHLKRVAAYCRVSSDSEDQKN
                     SYDSQVRHYKEYISQRSDWELADIYADEGISGTQVGKRQDFQRLINDCANGEIDYIVT
                     KAIARFARNTLDTLKYVRMLKDMQIGVYFEEENIDTLTMDGELLLTILSSVAQQEVEN
                     TSAHVKKGLKMKMQRGELVGFQGCLGYDYDVETKQISINKKEAKIVRYIFERYLEGIG
                     GKVIARELDELGYKSPRGLEHWNDTTVLGIIKNEKYKGDILMGKTFTVDPISKRRLSN
                     FGEEDKYYIKDNHEPIISKEDFEKAQEIRLRRAGNKKTAANVNGKRERYSKMYAFSSM
                     LECGFCGSILSRRSWHCRSDYRKVVWHCVTSIKKGKKFCKHSKGLEEIAIEGAFLEAY
                     RQVYHSNENLMTDLLETIESELNDNSLNKELKRITNKLRILLKKEENLVNLRLEGKVS
                     DSIYNEKYNEISSEKEFLAEEKVNIETTLKSEIDVKKRLTEFKHLLSSQKMLTEFDRA
                     VFESIVEKIIVGGVNSNGEIDPAMLTIIFKTGEIQNKDGKQFKSKRKNAKLETDKLCP
                     QNSDEDKKLYSQGTDNTRGVCSVAGSILASQ"
     gene            11456..12043
                     /locus_tag="VUW50_001734"
     CDS             11456..12043
                     /locus_tag="VUW50_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HET7983590.1"
                     /translation="MGGNPPMKKYSIVDKIVLSTKIKRIIIFTVFRENWEPYMKKYTE
                     VFQSQFPNLNIDYLLLDTEQIDLDSYLDADIIIIGGGNTEKYIATYVNQEFKSYIDHM
                     LNKEAKIIGFSAGALLLGEKVYVSPNDNSDHQIKIKNGLGLFSQFLISVHYDSWNDKA
                     NKDRAEELVNVPIIPLNDHSCLVLDKLGNIIEKID"
     gene            12283..12561
                     /locus_tag="VUW50_001735"
     CDS             12283..12561
                     /locus_tag="VUW50_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HET7983591.1"
                     /translation="MEKVASLGVPMCKPISIELCDDEVHSLHEWIDGRDAIDSILTYS
                     ENQQYTYGVEAGKILRKIHTIPATEVCEDWEIFFNLKIDDKISNEMIW"
     gene            12666..12995
                     /locus_tag="VUW50_001736"
                     /pseudo
     CDS             12666..12995
                     /locus_tag="VUW50_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            13123..14352
                     /locus_tag="VUW50_001737"
     CDS             13123..14352
                     /locus_tag="VUW50_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET7983592.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            14372..17068
                     /gene="ppc"
                     /locus_tag="VUW50_001738"
     CDS             14372..17068
                     /gene="ppc"
                     /locus_tag="VUW50_001738"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET7983593.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    17151..17368
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         5762 a         2608 c         3391 g         5627 t
ORIGIN      
        1 gggatggaat ttttcattta tcagagtgga aaaggaatga agaatttgac tttttcaata
       61 aaatggatgt tccttattta tctatgagtt cacgaataga agtaacgcag gctatcaatt
      121 ttcataaaaa acattcgata tcactttatg ccataatatc gtggtgtgtt atgagtgcaa
      181 taaattctat tccagaatta ctaatggata ccgacggaaa gattgtctgg caatacaatc
      241 aaagaggttg ttctttcact acccttacat cggaagataa gcttaatttt tcttcattta
      301 cgatgggaga taatttaatt gaatttgttt ctgcgttcaa cataaataaa cagaaggcag
      361 aagaagggca aaaaccaaat attgacaaga ataacattgc ttatctttct tgtgttccat
      421 ggattgattt tttacatgta agcactccta tgaatctttc aaaaattgat acagttcctc
      481 gaataacgtg gggaaaagtt attcaagaga accaaagata tttttgtaca gtgaatttac
      541 aaattaatca tggaatggga gatggattac acgtatctaa tttttttgtg ttattgcaaa
      601 ggtttgtaaa taaaataaat gaatattttc aaaaaaagta atgattgaaa agatattgca
      661 tttaaataat gttataataa aaataaagaa tttaaggaga aatacaatat gtcaattttt
      721 attggaggag catggccata tgcaaacggt tcgttacata ttggtcacgc ggcagcgctt
      781 ttaccggggg atattcttgc aagatactat cgtcagaagg gagaggaagt tttatatgtt
      841 tctggaagtg attgtaatgg aacccctatt tctatcagag ctaaaaaaga aaataagtct
      901 gtgaaagaaa ttgctgattt ttatcataag gaatttaaag aaacttttga aaaattaggt
      961 tttacatatg acttgtactc gcgaacagat tctccactac atcatgagat agtacaagaa
     1021 ttatttttgc aattatacga gaagaaattt ttgtatacaa aaaaaattaa acaactctat
     1081 tgtacatttg ataatcaatt cttacctgat aggttcgttg aaggaaaatg cccgaattgt
     1141 ggaacccatt caagaggaga tcaatgtgat aattgctcag caattttaga tcctatagat
     1201 ttagttgata aaagatgttc tatttgttcc aatgaaccag aagttcgtga gacagaacac
     1261 ttttattatg tttttagtga gtttcaaaat ttgttagaga cttacttaaa tgatgctgaa
     1321 gaaacagtga gatggcgaaa aaatgcaata aatctaacta aaaggtattt acgggaaggt
     1381 ttacctgatc gagctgttac tcgtgatcta ccaaatggga ttccagttcc cattgatggt
     1441 tttagagata aaaaaatata tgtctggttt gaagcggtag caggttacta cacagcaagt
     1501 gttgattggg cgcaaaaact tcaaaataat attactgatt tttggaacaa tcggacaaag
     1561 tcatattatg ttcatggaaa agataacatc ccatttcata ctattatttg gccggcaata
     1621 ctctcaggtt tagaaataga gcctcttcca gagtatatta tttctagtga atatttaacg
     1681 ttagaaaaca aaaaaatatc aacaagtaat aattgggcta tatggcttaa cgatatcata
     1741 aaaaaatatg atgctgattc aatccgatac ttcctaacaa taaatgctcc tgagatgaaa
     1801 gatgcaaatt tttcttggag agaatttatt tatagtcata actcagaatt attaggatcg
     1861 tacggtaatt ttatcaatag gactctaaag tttattgaaa agtattttga atctgaaata
     1921 cccactaaat atctcgaagg agaaatttta tataatttga aagaattata tacaacagta
     1981 ggtaatttag ttgagagcgg gcatatgaaa caggcattag aagaaatttt tgaatatatt
     2041 agatcggcta ataaattcta tgatgatatg aaaccgtggg cattgcgaga gtccgatatt
     2101 gaaaaatgta aagaagtctt agctacttgt gtaatcatta tacttaattt aggacaaatg
     2161 ttaaatccat tcattccttt ttctggtaaa aagattgagg atatgtttaa aacaaagcta
     2221 aatacttgga attatatttc aaaccttcct aacaagctat cagatgtatc aatgttgttt
     2281 gataggattg atttgaaaaa aattgacgag gaagttttag aattgcaaca aacatccagt
     2341 agataattca aaaatactcg aaagtagcct tttttaattt aaattaacaa ccggagatgt
     2401 tgaataaaat aaagatggtt ttttgagcat attatggaga aaagcattga ataatctaac
     2461 tttactaaaa gaatataatt ttagagatct tggaaaccac ctcactcaaa caggacaaaa
     2521 aataaaacct aaaacattat tccgttcaag taaactattt ggaatctcta aaattgatgt
     2581 cgatttgtta cagagctatg gaataacaaa agtcattgac tttcgatcag cgaatgaaat
     2641 aaaaaaagct ccagatccag atataaaaaa tataaaaaat atagtaattc caatattcta
     2701 taatgatgat tcagagttga cagaattccc aatagaattt tttaacaaat cagatgcagg
     2761 ttttcaacat atgataaaaa cttatgatca aatgatcaat caaaaacaga gtaaacttgg
     2821 ttacaagaaa ttctttaaat tattactaag tcatccaaaa gatgaatctc ttttgtttca
     2881 ttgttcaatg gggaaggatc gaacaggaat agctagcttg ttcttgcttt atatattggg
     2941 agtagatatg aacgatattt ttcatgatta cttactatct aacaagtatt taataaatgt
     3001 gagaaaagaa aatattgaat atgtaaataa ccatagtggg aatgtaatat tgatgcataa
     3061 tttactttca ctcagttcag caaaagaaga atatattaat cgagtattga atgtccttga
     3121 taaagaatat ggaggaatac ttagatatat taatacagaa ctaggaataa gtagtcaaga
     3181 aattgaggaa ttgaaagata gatatctttt ttagaatagt aagtactaaa aatgaatata
     3241 taacagtgcg aacggcggtt ttgtttttgt ctaattctac taatttaaca taagcaaaaa
     3301 cggattgttt tctatgtaag tccttaacta aattgatgga aagcacgtta aattatgtgc
     3361 aaaatgcgta caaaatagtg tttatcaaat gtaaaatttc ctacttgtca tcaaagttca
     3421 acaaatgtag gttgtttggt tatttgggta gatttctgga cttatcgttg gttgtagaga
     3481 cataatgttg cagttaaagc ttagagaata atctccgtat ttttgttagg caatttaaaa
     3541 gttaagacaa agtgagtttg tctcttaatt caactcacca caaacacatc acctttttat
     3601 tataacttcc gaccaatttt tttgaagaga aaaatgaaaa aacaccaact gtgcagggta
     3661 gctgacaagg cttctagctt ccatttattg aaaccaaatc aatctgttat gagataatag
     3721 cagttgcaga agctgactac tttgagaagt taaatgaggt ttattatcaa gaatacttca
     3781 tgaatatagt tataagggag ggggtagttg aaagaattga ctcatctgtt aaaataaaaa
     3841 tggaaataat aacatcataa tagtgtgact tagtattttt cagcatcttg gaagtaggta
     3901 aatgatatta aaattatact gcatagtaaa ggatagtaat gaggaattta atatcttcct
     3961 gtcaatatct tcagttatta aagtattttt atattgggat aacttcgttt gctgaaatct
     4021 atagtttatc caattataaa tttatatgtt cttcgtcata attctttgta cttgagtaat
     4081 ttagcttact tacagataaa atacaagatg gcatagtttt agtagctttt ttcatatggt
     4141 ttgtaatgat ggtaaatttt cttaacttta ctgtagtatg ctacaatttt tgttgatatt
     4201 ataacttgag tgaaacgaaa ggagtgattt ataaaaatga taacagtaga gcatcttcag
     4261 aaaaattttg tgaaaacaat aaaggaacct ggcttaaagg gagcttttcg ctcttttttt
     4321 catcccaaga gacaagtctt tgaagctgtt aaagatttat catttgaggt gcccaaagga
     4381 caaattttag gctttattgg cgcaaatgga gcggggaaat caacaactat caagatgttg
     4441 acggggattt tgaaaccaac atcaggtttt tgtcgtatca atggaaagat tcctcaagag
     4501 aaccgtcaag gctatgttaa agatattggt gtagtttttg ggcagcgaac tcaactttgg
     4561 tgggatttgg ctttgtagga gacttactcg gttttaaagg aaatttatga tgttcctgat
     4621 gcggtctttc aaaaacgcat ggattttcta aatgaagttt tagatttgaa ggaatttatt
     4681 caagatcctg tcagaactct atctctaggt caacgtatga gggcggatat tgcggcctcc
     4741 ttattgcata atccgaaagt tcttttccta gatgagccta ctattggttt agatgtttcg
     4801 gtcaaggata atattcgacg tgccattaca cagattaatc aagaagaaga gacgaccata
     4861 cttttaacga cccacgattt aggtgatatt gaacagttat gtgatcgtat ttttatgatt
     4921 gataagggcc gagaaatctt tgatggaact gtgaatcaac tgaaaaagac tttcgggaag
     4981 atgaagactc tctcttttga actacatcct gggcaggatt atattgtctc tcattttgaa
     5041 ggactttctg atatctatgt gacgagacag gaattgtcac ttgatattca gtacgacagc
     5101 tcgcagtatc aaacggctga cattattcaa caaactttgt ctgattttac gatccgtgat
     5161 ttaaagatga cggatgcgaa tattgaagat attattcgtc gcttctatcg aaaggagctt
     5221 taggatgact aaattatgga aacgctataa gccttttgtt agcgctggaa tccaagaatt
     5281 aatcacgtat cgtgtcaatt tttttcttta tcgcattggg gatgtgatgg gggcctttgt
     5341 agcattttac ttgtggaaag ctgtgtttga ttcatctcac cagtctctca ttcaaggttt
     5401 tactttatct gatatgacct tatatatcat catgagtttt gtgacgaatc tactgacaaa
     5461 atcagatagt tcttttatga ttggatggga agtaaaggat ggctctatta ttatgcgact
     5521 gcttcgtcca gttcattttg caatgtctta tcttttcaca gaaattgggt ctagatggct
     5581 agtttttgtt agtgttggtc taccatttgt aattctgatt gcaggtctaa agctcttatc
     5641 aggtgagtcc ttcttacaaa ttgttttaat aaccacagtt taccttctta gcttaatctt
     5701 agcttttttg attaattttt tttcaatatt tgctttggtt tttcagcttt tgtgtttaaa
     5761 aacttatggg gatcaaatct tttgaaaaat tctttagttg cctttttatc tggaagtttg
     5821 gttcctctga cattttttcc aaaaattatt gccgaactat tatctttttt gccattttct
     5881 tctttaatct ataccccggt gatggttatt atagaaaagt atagcatgag tcaaatgatt
     5941 caggcgctct cgctacaatt attctggctg tttattatga ttgccttatc ccaactgatt
     6001 tggaagtgcg tacagaatta tataactatt caaggaggtt agaatgaaga aatatcaacg
     6061 catgcacctg atttttatcc gacaatatct taaacagatt atggagtata aggctgattt
     6121 tttagttggt gttgtgggtg tctttttgac acaagggtta aacatgctgt ttctaaatat
     6181 tttgtttcaa catattccct tacttgatgg ctggagtttt catcaggttg cttttattta
     6241 tggtttttct ctcattccta agggaattga ccatctcttt tttgataatc tgtgggcttt
     6301 aggacaacat ttaattcgta aaggagaatt tgataagtat ttgactagac ccattagccc
     6361 tttgtttcat attttagtag aaacgtttca aattgatgct cttggagaat tgttagtagg
     6421 cgtcttattg ctgttaatga caataactag tcttacttgg acttgggcaa aggtttttct
     6481 ctttttaatc agtattccct ttgcaacctt aatttatacc tctttaaaaa ttgtaacagc
     6541 tagtattgct ttttggacta aacaatctgg ggcaataatt tatatttttt acatgtttaa
     6601 tgattttgca aaatacccga ttgcgattta ccattcgttt ttacgttggt tgattagttt
     6661 tatcattcct tttgctttta cagcctatta cccagctagc tatttcttga aagacaagga
     6721 tggattgttc aatattggtg gattgatcct tatctcatta atatttttta ccttatcatt
     6781 aaaattatgg aacaaaggtt tggatgcata tgagagcgca gggtcttaga agaagtaaaa
     6841 tctaattaag aattttgata gaacttccta ttctttggaa ggaagtttta atgatacatt
     6901 gagagctttg attaggctta ctgtagtttt tcaaacaaga aatttgacca ttgtgcaaaa
     6961 atgatacaaa ggtatgtaat atttcagtca taaccactcg ttcatatcat gagtggtttt
     7021 ttagctcttt tatacatacc atttgttaag ggacatacga tattctagct caaaaggttt
     7081 tctgaatgag tatcaaagga atgtttagaa gataattgcg agctgttttt tgaaatttgg
     7141 gcacacttaa aagacactta tctggtatta aaaaataagg tgttacaatg ataggacata
     7201 gatgacttta atgactttgg gttaaagtgt agtcgtaaag tttgttatgc gttatgaggt
     7261 aatacattgt ccggatgagg cgatgtatag agacaatcgt atatggcttg gttgaagtcg
     7321 tctgcgattg tctttttcgt ttctcataaa agtccgcgat atgacaagga ttggtatgac
     7381 tagctgaagc aatattgtgg acgcacttat agagaatttt tctcgcgtaa ggatttccac
     7441 gcttggtgat atgttcttga gctaggaaat tgccagattc atagtgtctg aggtcaatac
     7501 tgataaaggc attgatttgg ttggcagact gaaagcgacg aatgtctcca attcgccaat
     7561 aatgcttgta gctgttgttt ctgcgatacc aggaatagag agcagaatct cgtattcagg
     7621 taatggttca gccaattcaa tcatttgtgc taagtcctga tccttgcggt ttgtcagtct
     7681 caacagttcc tgtgcatgat acttaacctc ttctgtcatg ggagaggttt ttgaaatggc
     7741 aagataggac tgttcggcca gctcgctcaa cttttcagcc aatcgtccaa ctctgttctc
     7801 agaaatccgt ttagaagttg aattacgatg gcttctttta gctcaactac tgataatttc
     7861 agcctccaat gcttggttgg aaagatggtc acgaggttcc aatattgttc ccctgtagga
     7921 gtagagagaa ggttctttat ttctggaaag gcaatttgaa gcaccttgtg caggctattc
     7981 ttagtcctaa cgatgtcttc tgtcagattc tgatagaatc gactgaggtc acgcaaattt
     8041 tgatagactt cttcttgaac ataagttggt ttgcggttaa ggatgaattg tgacgaagct
     8101 agcttttcag cgtcaatctg attggtttta cggactcgca aactgtctag ttgcttctta
     8161 gttttaaagg atttagccgt atataagcat agctattgtc ctccagaaag gcctgaagac
     8221 gagaatagac ccctgtgcct cgaagataat ctcaggtgta tttacctatt tcaaatcctc
     8281 tagaagacga ctaaatccga tggcatcatt gggcatggta tagccatgaa ccttttcacc
     8341 attcacaaga atagcgactt ctgagcttgc cttactcaca tccattctga aaactgtccg
     8401 catcatgttt acccctttgt cttgaatgac tccttgtttt agtgatttta tttccaatac
     8461 tcgacgtcta gtatcccaca tactttgata aaattcttcc taaaacaggt ttcttgccag
     8521 tttgtcaagc gacgtctagc accatagaga gctctatgac ttaacaagac accactattt
     8581 taacacaaag aaaaagtaat gagtactctc tcccgttgga gatttcctca ctactaatct
     8641 tagtatgttt gtcaactgta gtgatagacg aataatcaag ttctggagaa ggtcagattg
     8701 ggcgattact tattattttc ctagcttgta cggtgctttt atcgctatga aaaagtttat
     8761 tccaaaatct ttttttagta acttatagct tacttactgt tataccaact atttctaaaa
     8821 tcttataaat tattttatgc atattttgca taaaattcca aatttgataa tcttcaaatt
     8881 acaatattag taagaggatt attaatgtat atcagattaa agaatttaag agaagacaaa
     8941 gatttgtccc agtgtcaaat tgcacagtat cttcattgtt cacagtctgc ttattctaga
     9001 atagaaagtg gcaagcaaga tattcctaca tcatttttga aacaattagc taaattatat
     9061 caggtaacta cagattatat tttagagatg gataagttta gttgttaata aatcctaagc
     9121 cctttgaaaa ctgaataaac cgaggtggga ctttcttatt tgtcgagcac tttagcttga
     9181 tacgccgtga taggagcgat aattattagc tataaaatag agtggttctc tatctgccat
     9241 gactacaaat aaggataatg atacttctga acgttcaggc tgccagcgaa aaaggacagc
     9301 gggtgaaatg cccatgaatg atttaaccag tcatacccac ggaggaaatt atacatgtta
     9361 caaggaggaa aaagtttgga acaatcagta ataaagtata ctatgcaact atcaatgctt
     9421 aactttttat actcaagaaa tatgttatct aaaaatgagt atgacaagat tagaaacaaa
     9481 ctgcgtatta agtacacaaa atagctgact ttttgatttg aatgcggtaa tatagaatca
     9541 aaaggaggaa tttcagtatg aaaaaaaata atgttgaaat aataaaagct gattctttag
     9601 ttcgtcgaag aggcgacaat gtagaaagac acttaaagag ggttgctgct tattgcagag
     9661 ttagttccga tagtgaggat cagaaaaaca gttatgattc tcaagtaaga cactacaaag
     9721 aatatatttc tcagcgttca gattgggagc tggcagatat ttatgcagat gaaggtattt
     9781 caggaacaca agtaggaaag agacaagatt ttcaacgatt aatcaatgat tgtgcaaatg
     9841 gagagataga ttatattgtc acaaaagcaa ttgctagatt tgcaaggaat accctagata
     9901 ctttgaagta tgtcagaatg ttaaaggata tgcaaattgg agtatatttt gaggaagaaa
     9961 acatagatac cctaacaatg gatggagaat tgcttctaac tattctaagc tccgttgcac
    10021 agcaagaagt agagaatact tctgcccatg tgaaaaaagg attaaaaatg aagatgcaac
    10081 gtggtgaatt ggttggtttt caagggtgct tagggtatga ttatgatgta gaaaccaagc
    10141 agatttcaat taataaaaaa gaagccaaaa ttgttcgtta tatttttgaa aggtatctag
    10201 aaggtattgg aggaaaagta attgcaaggg aacttgatga acttggttac aaatcgccaa
    10261 gaggtttaga acactggaat gatacaacag ttctaggaat aataaagaat gaaaaatata
    10321 aaggtgatat tctaatggga aaaactttta cagttgaccc aataagcaag agacggttga
    10381 gtaattttgg agaagaagat aagtactata tcaaggataa tcatgaacca ataatatcta
    10441 aagaagattt tgaaaaagct caggagatta gattgcgtcg agcaggaaat aagaagacag
    10501 ctgcgaatgt aaatgggaag cgtgaacgct attcaaaaat gtatgctttt agtagcatgt
    10561 tggaatgtgg tttttgtggt tcaatattat ctagaagaag ttggcactgc cgttcagatt
    10621 atcgcaaagt tgtgtggcac tgtgttacat cgataaagaa aggaaagaaa ttttgtaagc
    10681 atagtaaagg attagaagag atcgccattg agggggcttt cctggaagct tacagacaag
    10741 tctatcattc aaatgaaaat ttaatgacag acttacttga aacgatagaa tctgaattga
    10801 atgacaatag ccttaacaag gaactaaaaa ggattacaaa caaacttcga atattactaa
    10861 aaaaagagga gaatcttgta aatctaaggc tcgaaggaaa agttagcgat tccatataca
    10921 atgaaaagta caatgaaatt tcctcagaaa aagagtttct agcagaagag aaggtaaata
    10981 ttgaaacaac attaaaatca gaaatcgatg taaagaaaag actaactgag tttaagcact
    11041 tgctatcttc gcaaaaaatg cttacggagt ttgatcgtgc agtttttgaa agtattgttg
    11101 agaaaattat tgtaggtgga gttaatagca acggagaaat tgatcctgca atgttaacca
    11161 taatattcaa aacaggtgaa attcaaaaca aggatggtaa acaatttaaa agtaaacgta
    11221 aaaatgctaa actagaaaca gataaattgt gtcctcaaaa cagtgacgag gataaaaaat
    11281 tgtattctca aggaacagac aacacacgtg gagtgtgtag tgttgctggt tcgatcttag
    11341 ccagtcaata aaatgatatg ttttaaaaaa attataattt cgaaacgtga ataaacttgt
    11401 ttaaacttta tgtatcttta gaaagtgtga taacttgaaa gaacaaattt ttttgatggg
    11461 tgggaatccc ccaatgaaga aatatagtat tgttgataaa attgtattat caaccaagat
    11521 taaaagaatt atcattttta cagtttttcg agaaaattgg gaaccgtaca tgaaaaagta
    11581 cacggaagtt tttcaaagtc aatttcctaa tctaaacatt gattacttgc tcctggacac
    11641 tgagcagatt gatcttgata gctatctaga tgctgacata attatcatcg gtggtggaaa
    11701 tacggaaaaa tatattgcta cttatgttaa tcaggagttc aaaagttata tcgatcatat
    11761 gcttaataaa gaggcaaaaa ttataggatt ttctgcagga gccctactat taggagaaaa
    11821 agtctatgtc tcacctaatg ataattcaga tcatcagata aagataaaaa atggattagg
    11881 actctttagt cagtttttaa ttagtgtcca ttatgattcc tggaatgata aagcaaataa
    11941 ggatagagct gaagaactcg ttaatgttcc cataattcca ctaaatgatc attcttgtct
    12001 tgtattggat aaacttggaa acatcataga gaaaattgac taactttcat aatgtaaaaa
    12061 tggatctagg aaataaaagt gtagacgaac tggataggtt actagtgaaa actgcaaaaa
    12121 gtgttgtaaa gtgaatcgga gtgttttagt agtaagatag ccataaataa aggctggtca
    12181 gatgataaaa aatattgtgt gacagttcaa aatcagtaaa catatttctt gcgtgtttct
    12241 gataagtcga agttagatta taaaaaaatt gaatttgata cgatggagaa agtagcttct
    12301 cttggagttc cgatgtgtaa accaattagc attgaactct gcgatgacga agtacattct
    12361 ttacacgaat ggatagacgg aagagatgca atagatagca ttttaactta ttcagaaaac
    12421 caacaataca catatggtgt agaagcagga aagatactta gaaagattca tacaattcct
    12481 gctacagaag tttgtgaaga ttgggaaatc ttttttaatc taaaaattga tgataaaatc
    12541 tccaacgaaa tgatatggta agtccgaaac gctcagtgtg taagcttgtt gctaaaagcg
    12601 taaaaacctt ggaacgaaag gaataataga cttcctgcga aacaaaaata caatacaata
    12661 aaactatgga tgatgaagca agtaaacaat tgagcgatag ccgtttcaag atccttgtag
    12721 gtgttcagcg cacgactttt gaagagatgt tagctgtgtt aaaaacagct tatcaacgta
    12781 aacgcgcaaa aggtggacga aaaaccaaat taagcctaga cgatctcctt atggtaacta
    12841 ttcaatacat gcgagaatag cgcacttatg aacaaatttc ggctgatttt ggcattcacg
    12901 aaagcaactt aatccgtcgg agtcaatggg ttgaagtaac tcttgttcaa aatggtttta
    12961 cgatttcaaa ttctgctcta attatgaaaa acagtaaaat tcgaaggatt ataaggtaag
    13021 agtttttttc tttctgaaaa aatggtataa tagcaatcaa aactagaaaa taaaacggaa
    13081 tttggaacag atttgtctgt atcctagtag agtggtgatg ctatgaagat tagtaagagg
    13141 cacttattaa attattccat cttgattccc tacttgcttt tatctatttt gggcttgatt
    13201 gtggtctatt cgaccaccag tgctatttta attgaagaag gcaagagtgc cttgcagttg
    13261 gttcgaaacc aaggaatctt ttggattgtt agtttgatac tgattgcctt aatttataaa
    13321 ttgagactag attttttgag aaatgagcga ctaatcattt tagttatatt aatagaaatg
    13381 cttttattgt tcttggctcg ttttattggt atttcagtaa acggggcata cggttggatt
    13441 tcggttgcag gagtaactat tcagccagct gagtacttaa aaatcattat tatttggtat
    13501 ttagctcacc gattctccaa acagcaagaa gaaatagcta cttatgattt tcaagttttg
    13561 actcaaaatc aatggcttcc ccgtgctttt aatgattggc gattcgttct cctagttctg
    13621 attggaagtt tgggaatttt ccctgattta ggaaatgcga ctattttagt cttggtttcc
    13681 ttgattatgt atacagttag tggaatcgct tatcgctggt tttcaaccat tctggcgctc
    13741 gtatctgccg cttctgtctt tgtcttgacc actatcagcc taatcggtgt tgagaccttt
    13801 tcaaaaattc cagtattcgg ctatgtagcc aagcgcttta gtgccttttt taatcctttt
    13861 gccgatcgtg ctgatgcagg tcaccagtta gctaattctt attttgccat ggtcaatggc
    13921 ggttggtttg gtctaggtct tggaaactcg attgaaaaac gaggttattt gccagaagct
    13981 catacagact ttgtcttttc tatcgtgatt gaagaatttg gctttgttgg tgccagtctt
    14041 attttagctc tcttgttttt catgattttg cggattatct tggtcggtat ccgagcggag
    14101 aatcctttca atgccatggt tgcactcggt gtcggaggga tgatgttggt tcaggtattt
    14161 gtcaatatcg gagggatttc gggcttgatt ccatctacag gagtgacttt ccccttctta
    14221 tcccagggtg gaaatagtct tctagtctta tcagtggcag tagcctttgt cttaaatatt
    14281 gatgccagtg aaaaacgcgc taaattgtac cgagaattgg aaaatcaacc aatgaacctt
    14341 ctgttgaagt aggataaaga aaggatagtt tatgtctctt caaaaattag aaaattatag
    14401 taataaaagt gttgtgcaag aagaagtctt gattctaaca gaattactgg aagatattac
    14461 taaaaatatg cttgccccag agacctttga aaaaataata cagttgaaag aattatcaac
    14521 gcaggaagat tatcaaggtc taaaccgtct agtgactagc ttatcaaatg atgaaatggt
    14581 ctatatttca cgctatttct ctatcttgcc tcttttgatt aatatttcag aggatgtgga
    14641 tttagcttat gaaatcaatc atcaaaataa tattgatcag gactatttag gtaaattatc
    14701 tacaacgatt aaattggtag cagaaaagga aaatgccgtt gagatcctag aacacttgaa
    14761 tgttgtccct gttttgacag cccatccaac acaagtgcaa cgcaaaagta tgttggattt
    14821 aacaaatcat attcatagtc ttttgcgtaa ataccgtgat gttaagttgg ggttgatcaa
    14881 taaagataaa tggtacaatg atttgcgtcg ttacatcgaa attatcatgc agacagacat
    14941 gattcgtgag aaaaaattaa aagtgactaa cgaaatcacg aatgctatgg aatattataa
    15001 cagctccttt ttgaaagctg tacctcattt gacgacggag tataagcgct tagcgcaagc
    15061 gcatggtctg aatttaaaac aggctaaacc aatcaccatg ggtatgtgga taggtggtga
    15121 ccgtgatgga aatccatttg ttacagcaaa gaccttgaag cagtctgcac tcactcagtg
    15181 tgaagtcatc atgaactact atgataaaaa gatttaccaa ctttatcgtg aattttctct
    15241 ttcaactagc attgtcaacg tcagcaagca agtcagagaa atggctcgtc aatccaagga
    15301 taactcgatt taccgcgaaa aagagcttta ccgtcgtgcc ttgtttgata ttcaatcaaa
    15361 aattcaggca actaaaacct atctgattga ggatgaagaa gttgggactc gttatgaaac
    15421 cgccaatgat ttctacaagg atttgattgc cattcgagat tctctactag aaaataaggg
    15481 cgagtccttg atttcaggtg attttgtgga attattgcag gcagtagaga tatttggttt
    15541 ttacttagca tcaattgata tgcgacaaga ctctagcgtc tatgaagcct gtgtggcaga
    15601 actcttgaaa tcagcaggaa ttcattctcg ttatagcgag ttgagcgaag aagaaaagtg
    15661 tgaccttctc ttgaaagaat tagaagaaga tccccgaatt ctttctgcga ctcacgcaga
    15721 aaaatcagaa ttattagcaa aagaactagc tatttttaag acggctcgtg ttttgaaaga
    15781 taagttggga gatgatgtca tccgtcagac catcatttca catgcaacca gcctttctga
    15841 tatgctagaa ttagctattc tgttaaaaga agtaggactg gtggatacgg aaagggcgcg
    15901 tgttcagatt gttccccttt ttgaaacaat tgaagacttg gatcattcag aggaaacaat
    15961 gagaaaatat ctttctctta gccttgccaa aaaatggatt gactcacgaa ataactacca
    16021 agaaatcatg cttggctact ctgacagtaa taaagatggc ggttacttgt catcatgttg
    16081 gaccctctac aaggctcaac aacaattgac tgctattgga gatgaatttg gcgttaaggt
    16141 taccttcttc catggtcgtg gtggtactgt cggtcgtggt ggtgggccaa cctatgaagc
    16201 cattacatct caaccgctca agtctatcaa ggatcgtatc cgcttgacgg agcagggtga
    16261 agtaattggg aataaatacg gtaacaaaga cgccgcttac tataaccttg aaatgctagt
    16321 atcggcagct attaaccgta tgattactca gaagaagagc gataccaata ccccaaatcg
    16381 ttatgaagcc attatggatc aagtagtgga ccgtagttac gatatctacc gtgatttggt
    16441 ctttggtaat gagcatttct atgattattt cttcgagtca agtccaatca aggctatttc
    16501 aagttttaat attggttctc gtccagccgc tcgtaagact attactgaaa tcggtggatt
    16561 gcgtgctatc ccttgggtat tctcatggtc acagagtcgt gttatgttcc ctggatggta
    16621 cggggttggt tcaagcttca aggaatttat caataaaaat ccagagaata ttgctatctt
    16681 acgagatatg taccaaaatt ggcctttctt ccaatcgctt ctttcaaatg ttgatatggt
    16741 tttgtcaaaa tcaaatatga atattgcttt tgaatatgct aaactttgtg aagacgagca
    16801 agttaaggcc atctatgaga ctattttaaa tgaatggcaa gttactaaga acgttatctt
    16861 ggctattgaa ggacatgacg aactcttagc tgacaatcca tatctaaaag ctagtctgga
    16921 ttaccgtatg ccttacttta atattctcaa ctatattcag ttagagttga ttaaacgcca
    16981 acgtcgtgga gaattgtcca gtgatcaaga acgattgatt catatcacca tcaacggaat
    17041 tgcgacagga ttgcgtaatt caggttgata attttcaaga gtgaatgcta aaagtgaata
    17101 tcaaaaaaat tctaatagac tattgacaag tagtttaaaa atgatataat ttaaccattc
    17161 agaaaagtaa tcatacaaac tttttagaga gtctgtggta gctgaaaaca gataagtggc
    17221 aatgatgaaa attgggctga atgctattta gaatttgaaa ttataaaaat tcggtaagca
    17281 caccttacag tgcatctcgt tattgcgaga ctgagcgata gggaaattcc ctataattga
    17341 ggtggtaccg cgcatcgacg tcctcacaca agttttttgt gtgaggat
//
LOCUS       DAVDWT010000040        17032 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVDWT010000040 DAVDWT010000000
VERSION     DAVDWT010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17032
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.040"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            1..656
                     /locus_tag="VUW50_001739"
                     /pseudo
     CDS             1..656
                     /locus_tag="VUW50_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            653..871
                     /locus_tag="VUW50_001740"
     CDS             653..871
                     /locus_tag="VUW50_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983594.1"
                     /translation="MSISPRFETLEQAIAFKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(928..1212)
                     /locus_tag="VUW50_001741"
     CDS             complement(928..1212)
                     /locus_tag="VUW50_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HET7983595.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(1266..2201)
                     /locus_tag="VUW50_001742"
     CDS             complement(1266..2201)
                     /locus_tag="VUW50_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET7983596.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            2305..2748
                     /locus_tag="VUW50_001743"
     CDS             2305..2748
                     /locus_tag="VUW50_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HET7983597.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVG"
     gene            2864..3130
                     /locus_tag="VUW50_001744"
     CDS             2864..3130
                     /locus_tag="VUW50_001744"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET7983598.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREAVIFEKVRSRVEDKRYQETIVATFSDILKCSRDYQDQNIK"
     gene            3127..3501
                     /gene="crcB"
                     /locus_tag="VUW50_001745"
     CDS             3127..3501
                     /gene="crcB"
                     /locus_tag="VUW50_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET7983599.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            3495..3824
                     /gene="crcB"
                     /locus_tag="VUW50_001746"
     CDS             3495..3824
                     /gene="crcB"
                     /locus_tag="VUW50_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET7983600.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            3943..4290
                     /gene="rplS"
                     /locus_tag="VUW50_001747"
     CDS             3943..4290
                     /gene="rplS"
                     /locus_tag="VUW50_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET7983601.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            4333..4404
                     /locus_tag="VUW50_001748"
     tRNA            4333..4404
                     /locus_tag="VUW50_001748"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4366..4368,aa:Arg,seq:cct)
     gene            complement(4970..5824)
                     /locus_tag="VUW50_001749"
     CDS             complement(4970..5824)
                     /locus_tag="VUW50_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7983602.1"
                     /translation="MENFGAVLKDIRISKNFRLKDLSCNEISESTISRFENGITKLSI
                     NHFYILLNRLGISFSEFEELVHCYYSKKECLFEELEHAVNSSDIFLLQELVDKIELKQ
                     KQEKSLCNYHIKLIAEQQINRLANLPYNSSKCNELIKYLLSVDTWMEYELKLFYNSVF
                     FMNTRTISLLYRIVIKKTRYFLKTNTGTHRIIPLYLFNLKLLLKNNLLGSAQFFIDDL
                     ENLLTRQGYYFEKNYLLFLKGIYLIKTNQIELGKKECSKAMRIFKEYNDSDTINELNQ
                     KFKLDLTI"
     gene            5918..7138
                     /locus_tag="VUW50_001750"
     CDS             5918..7138
                     /locus_tag="VUW50_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HET7983603.1"
                     /translation="MKKILLSTVALLSLVASLLANNPVSAQESSSQATYSKSSGSWIK
                     SGNRWWYKHSDGSYTTNGWEKINGTWYYFDSEGWMKTGWIKEYGKWYYLDDSGAMKTG
                     WCLVSGSWYYLNSSGVMQTGLQTINGKQYYLAAGGAMQTGWHNIGDDTYFFANSGENQ
                     NINRRALVLGETSTRAVPIADVNAMEKVFNNQNFSEVVRFPDRTKSEIIAKMQELFES
                     SSEGDVNYLYFTCHGGRDGRIYIGSDGLAFSGWELASVLKQYKGKFVVMLDCCHAGTI
                     ISKDNTGEGNEGASTEYFDLDEFVSGFSNMDGNEKSGEMIDSKFLVLCSSRGAEYSSG
                     GSLSLATKYWSLGSGWNPLQNSQAYLAADQNNNRRITLNELYTYSREQVLKQNSNQHI
                     EVYPDNSQFVLFKK"
     gene            7151..8332
                     /locus_tag="VUW50_001751"
     CDS             7151..8332
                     /locus_tag="VUW50_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET7983604.1"
                     /translation="MKVSKKITLFSLSFAGFVLLTLPQAGKAFELKEDWAFKGGIRYE
                     NGKVSKINNGYEVNIKVLDLPSTSAIEWTVRLNGEKQNTNFLAEERTVSKTEDKGRFL
                     HFYIPYGYRGDIVVEAKSGNEVKTWSTKVVDDVYSDSAKSGYFILDGEQILESSWDSV
                     NESYIATLPTVTSGKTVVAWREKGTLNLIKPGRIARQYNSSGSYVELSPIFETASWLK
                     SNQNWYYQKQGQLVQNSWIKDQGSWYFMDDEGVMFNQTWLHQGGSWYAFKSSGAMISA
                     DWLYDNGSWYYLKDSGSMVTGWLKNGGSWYYLNKSGSMATGWIKDSGTWYYLKNSGSM
                     ATGWVKDSGSWYYLKNSGSMATGWVKDNGKWYYLASSGNMLRNTRTPDGYYVDGSGAW
                     K"
     gene            8818..10593
                     /locus_tag="VUW50_001752"
     CDS             8818..10593
                     /locus_tag="VUW50_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HET7983605.1"
                     /translation="MANNRKTETLGVSYLSTFIDKHELLQSYFESNDKTPVWDGEIHV
                     LKSPSEKKDEILGKVPVQIKTTRQKKDVLKSFSLDTRDLELYKPNGGVVLFVVWLNED
                     NGLRDIYYKSLPPLSIKNLLKKSKLKNKSTNRKKLSIEIFKLDEKKMYPMLVDFINNS
                     QKQYSFINVEGISVEDIPDDKTLKFYFYGQEKEEIFNYQEEHDLFIYYLDPITGIEIP
                     LENTIKIVETEEETDLIIKIGDYVFQDVKRHRFPDGSVQLHFGESFTMSFDIKKKQFK
                     FNYTRPDLLSKAIKCTQVFQELGKIGYFTLNGNKIELDERSIKDISSLDLEADIKGLL
                     KISNFMKKMGIQKDVDLSCFDKQSQRNLNILYSGLVLKKKVALNYNESKLLHLNIANI
                     HIITLYSFLSDKNGTMIDIFTETPWCREGETEDEDYLDISIFEVFEPNDWLKIDNCKI
                     DSVIASYQRLVDNKLKYEGADRTILKIVIAADMAEDKTKRELLLNWAQCLSDWNLKYS
                     KNCEMAIINDLQIKSRVRKLNSKETETLTNILVNSNDNYELCFGSSVLLKSKPQADLF
                     WNKLDNETKERYKDFPIYTLYMKLS"
     gene            complement(10804..11610)
                     /gene="yidA"
                     /locus_tag="VUW50_001753"
     CDS             complement(10804..11610)
                     /gene="yidA"
                     /locus_tag="VUW50_001753"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HET7983606.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDEGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMADKEIVKCMFIDEPE
                     ILDATIEKIPAEFYERYSINKSAPFYLELLTKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(11623..12927)
                     /locus_tag="VUW50_001754"
     CDS             complement(11623..12927)
                     /locus_tag="VUW50_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7983607.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            12999..13376
                     /locus_tag="VUW50_001755"
     CDS             12999..13376
                     /locus_tag="VUW50_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HET7983608.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELMMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            13467..13799
                     /locus_tag="VUW50_001756"
     CDS             13467..13799
                     /locus_tag="VUW50_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HET7983609.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            13811..15382
                     /gene="ffh"
                     /locus_tag="VUW50_001757"
     CDS             13811..15382
                     /gene="ffh"
                     /locus_tag="VUW50_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET7983610.1"
                     /translation="MAFESLTERLQNVFKSLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(15577..16860)
                     /locus_tag="VUW50_001758"
     CDS             complement(15577..16860)
                     /locus_tag="VUW50_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HET7983611.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
BASE COUNT         5422 a         2776 c         3596 g         5238 t
ORIGIN      
        1 ccatggttgg cgaaatatta tgccacgtcc agaacatcct aagaaagcag acgctcaaac
       61 cattgtcgcg tctaaaaata aaatctcaat tcaagaagac aagaaagcgt tttaaatata
      121 gtagacgttt tcataaggtt cgcttgatgt accaagatga ggctggtttc ggtagaatca
      181 gtaaactggg atcttgttgg tctccaatag gagtaggtcc acatgtccat agtcactata
      241 tacgagaatt tcgctattgt tatggagctg ttgatgccca tacaggcgaa tcatttttct
      301 taatagctgg tagatgtaat actgagtgga tgaacgcctt tttagaagag ctttcacaag
      361 cttatccaga tgattatctt ttattcgtta tggacaatgc tatatggcat aaatcaagta
      421 ccttaaagat tccgactaat attggtttta cctttattcc tccatacaca ccagagatga
      481 accccattga acaagtgtgg aaagagattc gtaaacgtgg atttaagaat aaagcctttc
      541 gaactttgga agatatcatg aatcaacttc aagatgtcat acaaggactg gagaaggagg
      601 tgataaagtc catcgttaat cggagatgga ctagaatgct ttgtgaaagc agatgagtat
      661 aagtcctcgt tttgaaacct tagagcaggc cattgccttc aaagatttgg aaaaagtccg
      721 agaagctttt aagaaaatga attccacttg gaccatcaat gaaagtgtgg ttcgtgacaa
      781 tagtacagct cattatggac gtgttgaaac agctatttcc ttcctgccta gtagcatgga
      841 aaccgaacct acagatgagt ctggaacatg aaaacagcag ttgatttcaa gaaacaaaaa
      901 agaacagtcg aaactgttct tttatcatta tttgagaccg tattttttgt tgaaacgatc
      961 cacgcgtcca tctgcttgag tgaacttttg acgtccagtg tagaatgggt gtgagtctga
     1021 tgaaatttcc acacggatca atgggtaagt ttcgccttcg aactcaactg tttcgttaga
     1081 gcgttttgtt gaaccgctaa ggaattggta accagtagtt gtgtccatga agacaactgg
     1141 gcgatattct ggatggatat cttttttcat tttgcaatat ttcctttctg ccatggtctc
     1201 tttgcgagcc atagattgtt accatactag tctatcagat tctgaaaagt ttggcaagta
     1261 ttttatcagt ttttaagcaa gttttttaac ttttggtaga tgatttcatt ttcttctagg
     1321 ctataggaat tagcaccact tgctagaggg tggcctccac catcatgctc cttggcgatt
     1381 tcattgatag gatggatttt actgcgtaag cgaactcggt agtggccatc agcctgttcg
     1441 acaaaaattc cccagagact cactctgtca atgcgtccag gtgcaccgac aatggctgca
     1501 gtttcagcat cggttacatt gtattgtttc aagacttctt gactcaggat aacgcgagca
     1561 gcaccatttt catccacttc cagatggtcg tagatgtagc cttgcagttt agcaattttg
     1621 tagctcatag tgtccatttt gcgagtgaga gctgcaaagt caaagttatg ttctctcaaa
     1681 taagcagcca ggcgaagagt ccgtgcagtg gtagaagggt agaggaagcg acctgtatca
     1741 ccgacaattc ctgcaaagag caactcagca tcgcgatctg ccaaggctag ttgggttgtt
     1801 tgggcaaata gggtaatcat ctcgctagcg ctacttgaac tagtatcgac ccaagacagg
     1861 tcaccgtata catcatcatt tggatggtgg tcaatcttaa tgagaaaatc accttgacta
     1921 tagcgcttat catcgatacg agcagtatta gctgtatcac agacgatgac aagtgcgcct
     1981 tggtaggctc tatcttcaac aagatccatc tcagccatcc aagtaagagt tggttcatca
     2041 aaaccgacgg ctttgatggt tttttctggg aaatgatgtt ctagcaaggc tttcaatccc
     2101 acctgacttc ccaaggcatc agggtctggt ttcatatgac gatgaatgat aatggtgtcg
     2161 tattctttaa ttttttctaa aatttgttgg caaatctcca taaataacct caattctttc
     2221 tcatttcatt gtagcacaag aatttttatt tttaaaatga aaaagatgtg atataatgga
     2281 gaaagataaa ttgaggagga cgatatggca ttagcaaaaa ttgtatttgc cagtatgacc
     2341 ggtaataccg aagaaattgc agatattgta gcagacaaat tacgtgactt gggcttggat
     2401 gttgatgttg atgaatgtac aactgttgac gcttcagact tcttggaagc agacatcgct
     2461 atcgttgcga cctatactta tggtgatggt gaattgccag atgagatgat ggacttctac
     2521 gaagacctag cagatctcaa cttgaatggc aaaatctacg gagtggtcgg ctcaggagat
     2581 accttctacg atgaattctg taaggctgtt gatgactttg accgtgtctt tgtgtcaaca
     2641 ggagcagaaa aaggttcaga gtgtgttaaa gttgatcttt ctgccgagga agaagatatt
     2701 gaacgcttgg aacaattcgc agaagaattg gttgctaaag taggataagc ataaaagtgc
     2761 gctgatgtaa tcaatcagtg catttttctt atcgagagtt gggattttac tagcctaatt
     2821 gatggctttt tgatacaata attcaaataa aggaggagac tgtatggatt tagatattat
     2881 tcgtcaagaa atcgatcaaa tcgacgacca aattgtcaaa ctgttagaag aacgaatgca
     2941 cttagttgaa ggagttgtcg cttataagaa ggcttcaggg aaaccgattt tagatactaa
     3001 gagagaagca gttatttttg aaaaggttag aagtcgtgta gaggacaagc gctatcagga
     3061 gactattgtc gcgacttttt cggacatact caaatgttcg cgtgattatc aggatcaaaa
     3121 tatcaaatga aaaaagaaca attttatccg ctagggattt ttctagctgc tatgttgggc
     3181 ggacttgtcc gctacctagt ttccacttgg ttaccagcca gtccagattt cccttggggc
     3241 actctctttg tcaactatct gggaattttc tgcttgattt atcttgtcaa gggctatctg
     3301 gtctataagg ggactagtaa gggcttgatt ttagcactgg ggacgggttt ttgcggaggt
     3361 ttaacaactt tttccagcct aatgcttgat actgtgaagc tgcttgatac agggcgttat
     3421 cttagtttga tactgtattt gcttttgagc atcggtggag gcctactctt agcttatttt
     3481 ctagggagga agaaatggta atcgtctatc ttgcaatcgc ctgtggcttg ggagcactcg
     3541 tacggtattt cttttcccgc tacaatcaag cttctaaatt gccactcgga acgctcatag
     3601 ctaatctttt aggttgtttt ttaattggag tattctacaa tcatgtagag tctaaggaag
     3661 tatatgctat tctagcaaca ggattttgtg gcggtttaac aactttttcg accttgaatg
     3721 acgaacttca aagactgcta agtgataaga aggtctttta ttcttacctg actttgactt
     3781 acataggtgg tttggttgcg atttttttag gaattttgct ataaatttct ttactttttt
     3841 gtgaaaattt ggtagactgt atcttgtgtg tgatggacgc acaaaaatac agtttatccg
     3901 ctgaggaagt ttcctcaaga ttgacaaaga taggagaata aaatgaatcc attaatccaa
     3961 agcttgactg aaggtcaact tcgtacagat atcccatcat tccgtcctgg tgacactgtt
     4021 cgtgtacatg cgaaagttgt cgaaggtaac cgtgaacgta tccagatttt tgaaggtgtt
     4081 gttatcgcac gtaaaggtgc tggaatctca gaaaactaca cagttcgtaa aatctctaac
     4141 ggtgtaggtg ttgagcgtat cttcccaatc cacactccac gtgttgaaaa aatcgaagtt
     4201 gttcgttacg gtaaagtacg tcgtgcgaaa ttgtactact tgcgtgctct tcaaggtaag
     4261 gcagctcgta tcaaagaaat ccgtcgttaa ttcgactaaa aaactctgct ttttcagcag
     4321 agtttttatc tagctccctt agttcaatgg atataacaac tccctcctaa ggagtagttg
     4381 caggttcgat tcctgcaggg ggcattttta gtaatgtaaa gaaagacaaa gaaaattcaa
     4441 aatgtttatt tgaaggcaat tttctcaagt tcatttttaa aaataattta gcatttttaa
     4501 tttttttgac caaattttga ccaaaaatta tattttggtc aatgattata gaataaaatg
     4561 gatcataaaa atgtctttga tttatagtag tgctaaaaag gacttccttt attggaatgt
     4621 ccttattttt attatggaat tgccaaatga cgttaaggtt cagttctatg aacttagaaa
     4681 gaaagtacaa agctttaatc agctttcaaa aagatttggt atggatgttt ctggctaagg
     4741 tatgtgacaa ggtgaattga tcgttattga acagagagcg ttaaaaaagg gaagaatcgt
     4801 tactattcca ctgaattaaa acaagaaata aatcaacaaa agcctatata ataacaaatg
     4861 tattaaaaat aaaaagattt agttcagtgt aatgcagaac caaatcttat ggataaatta
     4921 tttgtctaat tttttaggaa ttatatctct aaacaatatc atttaacaat taaattgtta
     4981 gatctagctt aaatttctgg tttaattcat ttattgtgtc actatcatta tattccttaa
     5041 atattctcat agctttagaa cattcttttt tacctaactc tatttgatta gtttttataa
     5101 gataaatacc ttttaggaag agtaaataat tcttttcaaa ataatatcct tgcctagtaa
     5161 gcaaattttc taaatcatct ataaaaaatt gagcactacc taataaatta ttttttaata
     5221 ataattttag gttaaataaa tacaggggaa ttattctatg cgttccagta ttagttttta
     5281 aaaaatatcg tgtttttttg attactattc tatatagtaa gctaatggtt ctagtattca
     5341 taaaaaacac tgaattatag aaaagtttta gttcatactc catccaagta tctacagaaa
     5401 gcagatattt tattaattca ttacatttag atgaattata aggaaggttt gcaagacgat
     5461 taatctgttg ttcagcaatc aattttatgt gataattgca taaacttttt tcttgctttt
     5521 gttttaactc tattttatcc actaattctt gaagtaaaaa tatatctgaa gaatttacag
     5581 catgttctaa ttcttcaaaa agacattctt ttttggaata ataacaatga actaattctt
     5641 caaattctga aaatgatatt ccaagacggt ttaacagaat ataaaaatga ttaatagata
     5701 attttgtaat gccattttcg aaacgagaaa tagttgattc actaatttca ttacatgata
     5761 aatctttaag gcgaaaattt tttgaaatac gaatatcctt taaaactgct ccgaaatttt
     5821 ccataatgtt cctcttccat ttctgcaaaa aataaaacgc ttgtttttta tttatgatat
     5881 gattatacaa aaataaaata gaaaggacaa taatatcatg aaaaaaattt tattgtcaac
     5941 agttgcttta ttaagcttag tagcatctct gctagcaaat aatccagttt ctgctcaaga
     6001 atcatcctct caagcaactt atagtaagtc aagtggaagt tggataaaat ctggtaaccg
     6061 ttggtggtat aagcattctg atggttctta tactacaaat ggatgggaaa aaataaatgg
     6121 tacctggtac tattttgata gtgaaggttg gatgaagaca ggttggatta aagaatatgg
     6181 gaaatggtat tacctagatg atagtggtgc tatgaaaact ggatggtgtt tggtttctgg
     6241 tagctggtat tatttaaata gtagcggtgt aatgcaaacg ggactacaga ctatcaatgg
     6301 aaaacaatat tatttagctg ccggcggtgc tatgcagaca ggttggcaca atatcggaga
     6361 tgatacctat ttctttgcta atagtgggga aaatcaaaat atcaatcgtc gtgctttagt
     6421 tcttggtgaa acatcaacga gagcagtacc tattgcagat gttaatgcta tggaaaaggt
     6481 ttttaataac caaaacttta gtgaagttgt tcgtttccca gatagaacaa aatccgagat
     6541 aattgcaaaa atgcaagagc tttttgaatc ttctagcgaa ggcgatgtga actatcttta
     6601 ctttacttgt catggaggaa gagatggaag aatctatatt ggtagtgatg gcctagcatt
     6661 ttctggttgg gaattggcgt ccgttttgaa acaatacaaa ggtaagtttg ttgttatgct
     6721 ggattgttgt catgctggaa caattatttc taaagataat acaggtgaag gcaatgaagg
     6781 agcctctacg gaatattttg atttagatga atttgtatca ggattttcta atatggatgg
     6841 taacgaaaaa tcaggtgaga tgattgactc taaattttta gtattatgct catcaagagg
     6901 tgcagaatac tctagtggtg gatcattaag tttagcgact aaatattggt ctttaggttc
     6961 aggatggaat cctctacaga attcacaagc atatttagca gcagaccaaa ataataatcg
     7021 cagaatcaca ttaaatgaat tgtatacata ctctcgtgaa caagttttga aacaaaattc
     7081 taaccagcat attgaagttt atcctgacaa cagtcaattt gtgttattta agaaataagg
     7141 gggcaataaa atgaaagtat caaaaaaaat tacactattt agtttgtctt ttgcaggttt
     7201 tgttttattg actttacctc aagcaggaaa ggcttttgaa cttaaagaag actgggcatt
     7261 taaaggtggc attcgatacg agaatgggaa agtcagcaaa attaataatg gatatgaagt
     7321 aaatattaaa gtgttagatt tacctagtac tagcgcaatc gaatggacag ttagattgaa
     7381 tggagaaaag caaaatacta acttcttagc ggaggaaaga actgtatcta aaactgaaga
     7441 taagggacgt ttcttgcact tttatatccc ctatggatat cgtggggata ttgtagtaga
     7501 ggctaagagt ggaaacgaag tgaagacttg gtctactaag gtagttgacg atgtttattc
     7561 agattctgct aagagtggct actttattct cgatggggaa caaatcttag aaagttcatg
     7621 ggattccgta aatgagtctt atattgcaac gcttccaact gtaacatcag gaaaaactgt
     7681 tgttgcttgg cgtgaaaaag gaactcttaa tttaattaaa ccaggaagaa ttgctcggca
     7741 atataatagc agtggatcat atgtagaact ttctccaatc tttgaaactg ctagttggct
     7801 aaaatcaaac caaaactggt actatcaaaa acaaggtcaa ttagttcaaa attcttggat
     7861 caaggatcag ggaagctggt actttatgga tgatgaaggg gttatgttta atcaaacatg
     7921 gctacaccaa ggtggaagct ggtatgcctt taagtcatca ggagccatga tttcagctga
     7981 ttggttgtat gataatggtt cttggtatta cctaaaggac tcaggttcta tggtaacagg
     8041 atggcttaaa aatggaggct cttggtatta cctaaacaag tcaggttcaa tggctacagg
     8101 ttggattaaa gatagtggaa cttggtatta tctaaagaat tcaggctcta tggcgacagg
     8161 ctgggtgaaa gatagtggct cttggtatta tctaaagaac tcaggttcaa tggcaacagg
     8221 ttgggtcaaa gataatggta agtggtacta tctcgcatca tctggtaata tgcttcgcaa
     8281 tacacgcaca ccagatggct attatgtaga tggcagtggc gcttggaaat aaatttttaa
     8341 aagagaaaat ctactgagaa ggtagatttt ctttttagtt aatgaatata gaataaagta
     8401 gatcaggact ggtctatttt attttgtaac tattaatgaa tagtcttaat gatgaagtta
     8461 ctttctgaag atatttaatt gtgttgagat ttgataaaac tatagcggat tgaaataaga
     8521 tataaacaaa gagattatgg aagtcaaatt aatttctaaa aacgttttaa aatccttagc
     8581 gtactatttt agattcaatt taatatagtt taaaaataag aaaattacgt accttattct
     8641 tggtgataaa aatgtgaatt tagataaata tatttgattt gtagaaggtg ctcataatcc
     8701 atattttaaa atttatacta ttatactgaa atttaattgt agtttattga atgctatgta
     8761 tttttatttc ttaatgctat aatataaagt atatagagtt attttagagg tgcagatttg
     8821 gccaataata ggaaaactga gacattaggt gtttcatatc tttcaacatt tatagataag
     8881 catgagttac ttcaatctta ttttgaaagt aatgataaaa ctccagtatg ggatggagaa
     8941 attcatgttc ttaaatcacc gagtgagaag aaagacgaaa tattaggtaa agtacctgtt
     9001 cagattaaaa ctactagaca gaaaaaagat gtgttaaaat ccttctcatt agatactaga
     9061 gatttagaac tatataaacc aaatggtgga gtagttttat ttgtagtttg gttgaatgag
     9121 gataatggtt taagagatat ttactataag tcattacctc ctctttctat aaagaatttg
     9181 ctcaaaaaga gtaaactaaa aaataaatct acaaatagga agaaactttc catagagatt
     9241 tttaaactgg atgagaaaaa aatgtatcca atgttagtgg atttcataaa caatagtcag
     9301 aagcagtata gtttcataaa tgtcgaaggt atttcagttg aggacattcc tgatgataaa
     9361 actctaaagt tctacttcta tggtcaggaa aaagaagaaa tttttaacta tcaggaagaa
     9421 catgatctct ttatttatta tttagatcct ataactggca tagaaatacc tcttgaaaac
     9481 acaataaaga ttgtagaaac tgaagaagaa acggatttaa ttataaaaat aggcgattat
     9541 gtttttcaag acgtaaaaag acatcgtttt cctgatggga gtgtacagct tcattttggt
     9601 gaaagcttta caatgtcttt tgacataaag aagaaacaat tcaaatttaa ctatactagg
     9661 ccggatttgc tgtcaaaagc tataaagtgt actcaggtat ttcaagagct aggaaaaatc
     9721 ggatatttta cccttaatgg taataaaata gagttagatg aacggtcaat aaaggatatt
     9781 tcttcacttg atttagaggc tgatataaag ggattgttaa aaatttctaa ctttatgaaa
     9841 aaaatgggta tacaaaaaga tgttgattta tcttgctttg ataaacaatc tcaaagaaat
     9901 ctaaatattc tttattcagg tcttgtctta aaaaagaaag ttgctttaaa ttataatgaa
     9961 tcgaaattac ttcacctaaa tattgctaac atccacatta taactttata tagttttttg
    10021 tcagataaaa atggaactat gatagacatt tttactgaaa ctccttggtg ccgggaagga
    10081 gaaactgaag atgaagatta tttagacatt tcaatttttg aagtatttga accaaacgat
    10141 tggctaaaaa ttgataattg taaaattgat tcagttattg cctcgtatca aagattggta
    10201 gataataagc tgaaatatga aggcgctgac cgtactattt taaaaatagt tatcgctgca
    10261 gacatggcag aagataagac taaaagagag ttgttactta attgggcgca atgtctttct
    10321 gactggaact taaaatactc aaaaaattgt gagatggcta taataaatga ccttcaaata
    10381 aaatctagag ttcgtaaatt aaacagcaaa gaaacagaga cactaactaa tattttagta
    10441 aatagtaatg ataactatga actttgtttc ggttcatcag tgctattaaa atcgaaacca
    10501 caagctgatt tgttttggaa taaactggat aatgagacaa aagaaagata taaggacttt
    10561 cctatttata ctttgtatat gaaattatcg taaaattaaa agagattata aagagataac
    10621 atatagaaat agagagtgat ttattacaat tcagtaccac aaattaagaa agcaatgcct
    10681 attttttgta ctctcaagat ttaagtaact gtacaactgc aattaaattt attaatttac
    10741 tctcattgtg atatattcat acaaaaaccg ctcagctgag cggttttagt ctattctttc
    10801 cttttacagt acccatgttc ggatggcgtg ggcaacgccg gattcgtcat ttgttttagt
    10861 gatgtatttg gcgatttttt tgatttctgg atttccattt tccatgacaa cggggtttcc
    10921 aacgacttcc agcatggcac ggtcattttc ttcgtcaccg attgccatgg tttcatcttt
    10981 ggtcaatccg agtttttcag ccaagtgagt aatggctgaa cccttgtcta cattctttgt
    11041 aaggagttcg aggtagaaag gagcagattt gttgatggag tagcgctcgt aaaattctgc
    11101 tggaattttt tcaatcgtag catcgaggat ttctggttca tcgatgaaca tacatttgac
    11161 aatttccttg tcagccattt cttcaggtgt acggtagaag ataggcatgc tgacgagggt
    11221 tgattcgtgc acagtgtatt ttccgatatt gcgattggca gtatagatac cgtccttggt
    11281 aatggcgtgc atgtggacac cgagcttgcg gctgagaaat tccatatcta gataatcctc
    11341 ataagtcaag gattcgctga taatctcatg tcctgtagca gtttcttgga caagggcacc
    11401 gttgaaggtt accacatagt ccccctcgtc tctcaactgc aagtcgtcta gaagtttggc
    11461 aacgcctgcg atagggcggc cagttgcaat cacgactttg acaccagctt ctttggcatc
    11521 ttggatggca gaaaagactt caggagtgat ttccttttgg ctgttgacta gggttccgtc
    11581 aatatcgacg gcgattagtt taatactcat aaatttcctc ttttagtttt tatttggggt
    11641 aaaatgatcg ttctcaatca agtgtaaaaa ttgctgggtg atgcttgcga agatgctgtt
    11701 ttggtctaac atttctttcg ggaaataaaa gcgattatct ccgtggcgac ttccagcaag
    11761 ggattggacg ataggggaca ggctagagag ttctgccagt tctccatttt tttgtaaaat
    11821 ctcaatctgt gtccttggat tttcagattc gggacggtaa atatcataag ggaggtcaaa
    11881 gttcttatga atggcagtat agtagtcggg atcaaagccg atgtcctcaa ccaattttct
    11941 catgctagcg agttggtctt ggtcttcttg tgaaaaggta atggatttaa agaccttgcg
    12001 gttgacaaag cgatgcgata aatctgcaag aatcttgtca ggactggtca tccagagttg
    12061 gaagtaggta ttcatcacac catcatccag agccagatag tcagtcaagg tcacattttt
    12121 ttcgaagaaa ggcaggaggt gtggagaagt tcgggcaaag aaatccttgt cctcaggata
    12181 gagttccttg gcgcgtttga gaagattctg taggagaact tccatggcgc gtgttgcggg
    12241 gtggaaataa acctgcatgt acatctggta gcgactgagg acgtagtctt cgatggcgtg
    12301 catgccattg cgctgaaagg cgataccatt ttcgatagga cgaatgactc ggaggattcg
    12361 agtcaggtca aattccccat aggatgctcc tgtaaaatag gagtcgcgca agagatagtc
    12421 catgcgatct gcgtcaatct gactagaaat gagctgcacg acctgcttgt taggataggt
    12481 atggtctatg acactggcaa ctttctctgg aaaatcgggc gctacttgta gcaagacttg
    12541 gtgaatctct gtctcaggat tttgaataat ctcctgagta atggcttcat ggtctgtatc
    12601 aaagagattt tcaaaagtat gggagtaggc accatgccca aggtcgtgta ggagagcagc
    12661 ggtcatggtc aagagagact cggcaggatt ccattcctca ggatattttt cttcgaaaat
    12721 ctctgtgatg cgtcgtgcaa tttcatagac tcctagacag tgagagaagc gactgtgttc
    12781 tccaccgtgg aaggtataac tggaagttcc cagttgtttg atccggcgca aacgctgaaa
    12841 ttcttttgta ttaatcaagt catagatgat ttgattattg acatggatgt agttgtgaac
    12901 agggtcacgg aatacttttt cgttcatatg accattatag caaaatcctg ctctttttgg
    12961 taaaatagta ggacaagaga caagaaagag ggcctgatgt gaagattcgg atgcgaaata
    13021 caattcagtt tgatgagcag ttggaagtga ttgaccagct ttatgatgtg gaagtgcatg
    13081 aaaaaggaga ttatagctac ctgcttttct ataatgagga aaaggaaaaa gtagtgatta
    13141 aatttcatgg tcaagaactt atgatgagcc gattttctaa tcccaagacc attatgcgct
    13201 ttctaaagga tagcgatagt ttagcctata ttcctacacc tatgggcatg caggagttta
    13261 tcatccaaac gagccattat caagttgata ggcaaaagat tgaactagat tatcaactac
    13321 aaaatcaaga gggacatcct tttgccagct atcaattgga aattacttgg ggctaggctc
    13381 atgtcttttt caaatttagc tatctatctt cttggatcgg cttcctagtg tggtagatta
    13441 ggctaatttt tggtataata aaagttatgg aaatcgaaaa aaccaatcgt atgaatgcgc
    13501 tctttgaatt ttatgcggcg cttttgacag ataagcaaat gaattatatc gagctctact
    13561 acgctgatga ttacagcctt gctgaaattg ccgaggagtt cggtgtcagt cgtcaggctg
    13621 tctatgacaa tatcaagcga acagaaaaga ttctggaaga ttatgagatg aaattgcaca
    13681 tgtactcgga ttatattgtc cgcagtcaga tttttgatca gattttggag cgctatccca
    13741 aggataactt tctgcaggag cagatagaaa ttttaacaag cattgataat agagaataag
    13801 aggaagaaaa atggcatttg aaagtttaac agaacgtttg cagaacgtct ttaaaagtct
    13861 acgtaaaaaa ggaaaaatct ctgaatctga tgtccaagag gcaaccaaag aaattcgctt
    13921 ggccttgctc gaggccgacg ttgccttgcc tgttgtaaag gactttatca agaaagttcg
    13981 tgagcgtgca gtcgggcatg aggtcattga tacacttaat cctgcgcaac agattattaa
    14041 aatcgttgat gaggaactga cagctgtttt aggttctgat acggcagaaa ttatcaagtc
    14101 acctaagatt cctacaatca tcatgatggt tggtttgcag ggagctggta aaacaacttt
    14161 tgcaggtaag ctggctaaca aactcaagaa ggaagaaaat gcccgtcctt tgatgattgc
    14221 ggcggatatt tatcgtccag ctgccattga ccagcttaag accttgggac aacagattga
    14281 tgtgcctgtc tttgcacttg gaacagaagt accagctgtt gagattgtac gtcaaggttt
    14341 ggagcaagcc caaactaatc ataacgacta tgtcttgatt gatactgcgg gtcgtttgca
    14401 gattgatgag ctcctcatga atgagcttcg tgatgtgaaa gcattggctc aaccaaatga
    14461 aatcttgctt gtcgttgatg ctatgattgg tcaggaagca gccaatgttg cgcgtgagtt
    14521 taatgctcag ttggaagtga ctggggtcat ccttaccaag attgatggcg atactcgtgg
    14581 tggtgctgct ctgtctgttc gtcacattac tggaaaacca atcaagttca ctggtacagg
    14641 tgaaaagatt acggacattg aaaccttcca cccagaccgc atgtctagcc gtatccttgg
    14701 tatgggggat atgctcactt tgattgagaa agcttctcag gaatacgatg aacaaaaagc
    14761 ccttgaaatg gctgagaaga tgcgcgaaaa cacctttgat tttaatgatt tcatcgatca
    14821 attagatcag gtgcaaaata tggggccgat ggaagacttg ctcaagatga ttccaggtat
    14881 ggccaacaat ccagcccttc aaaacatgaa ggtggatgaa cgccagattg ctcgtaaacg
    14941 tgccattgtg tcttcgatga cacctgaaga gcgtgaaaac ccagatttgt taaatccaag
    15001 ccgtcgccgt cgtattgctg ctggttctgg aaatacattc gtcgaagtca ataaattcat
    15061 caaggacttt aaccaggcta aacagctcat gcagggtgtt atgtctgggg atatgaataa
    15121 aatgatgaag caaatgggga ttaatccaaa taaccttcct aaaaatatgc caaatatggg
    15181 aggaatggat atgtctgccc ttgaaggaat gatgggacaa ggcggtatgc ctgacttatc
    15241 agctctcgga ggagcaggaa tgccagatat gagccagatg tttggtggcg gtttgaaagg
    15301 taaaattggt gaatttgcca tgaaacagtc catgaaacgt atggctaaca aaatgaagaa
    15361 agcgaagaag aaacgcaagt aagacaagag attgatgcaa atctcatttg atgagaaaat
    15421 aaagtaaaaa tttctatgat aaaacgtaca gtatcaaggt tttagaatat ctgatattat
    15481 acgttttttg attttaataa ttttgttaag acacttttat tatggtatgc gaaaaatcca
    15541 ctcagctgtc tgagtggatt aggtgtattt agactcttag tctttatttt cgtatggcaa
    15601 gatcaagttc aagatgattc ctgtcatggc tgaaagggca gtacctgaaa gtgtaactgg
    15661 accaagttta aggatagctc ctccaagtcc aagaaccaac atagcacttg cgatgatgag
    15721 gtttcgcatt tgagcgaaat caacacgttc tttaatcaag actttcaaac cattgctggc
    15781 gataacccca tagagaagga ttgacatacc accaagtaca gcgtttggaa tagttgaaat
    15841 caaggcagtg aatttaccaa ggaagctgag ggcaatcgcg atgaaggcag cgttacggat
    15901 aactgagaca gaagcgatac gagtcatacc gataacccct gtattttctc cgtaagttgt
    15961 attggctggt ccaccaagga aggcagaaac agaagttgcg ataccgtcac caagaagagt
    16021 acgatgaaga cctggttctt ttaagaattg acggccacag atttgaccca aaacagtatg
    16081 gtctccgata tgttcagaaa ttgttacgat agcgattggc aagatagcga tggcttctgg
    16141 accaaagtaa agattgtact ctttaaaggc accacctgtg ctaaatggca agtagaaacc
    16201 aggaatttcg aaccagttgg ctttaagaac tggtgtaaag tcaaccaagc caagagttag
    16261 tgcgaaaagg taaccaccga taatggcaaa gaggaatgga atgattcgta ggaagccttt
    16321 tccttttgta ttgataaagg cagcaattag gaaagtaaca acggctacca gagcattttt
    16381 ccaatttccg tctgctacaa gacctgcatt ggtaacagct gaacctgcaa gtccaagacc
    16441 gataacgatg atcataggac cgataatgat tggtggcaag agtttatcaa tccattttgt
    16501 tcctacaaat cggatgctgg tagcaacaag gacatagacc aaaccagtca agataacccc
    16561 tgtttgggca gcagatacat caccccccat ttctttcata gccagtgaca tagctgtgat
    16621 aaaggcaaat gaagaaccta gataaactgg aactttaaaa ccagtagcaa tcatgtagat
    16681 gagtgttcca acacctgaag caaaaagggc aacagataca ggcattccca aaatcaatgg
    16741 taccaagatg gtcgcaccaa acatggcgaa aacgtgttgg aagctaagga gaattccttt
    16801 tccatccgaa ggacgttggt caacgtctag taacaaatca acagttgatt cttgtttcat
    16861 ataaacctca cttttgggca tcagaaaaga gcccattatt ttacagcaat aggccctcag
    16921 agtaagactt ctctaaaatc tagactttaa agagtttcaa atatttttgc gtcttcgtca
    16981 cctcacggga tgacattaaa aacctttgct taggatagta tagcagaaaa aa
//
LOCUS       DAVDWT010000041        14497 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVDWT010000041 DAVDWT010000000
VERSION     DAVDWT010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14497
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.041"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            473..1594
                     /gene="mnmA"
                     /locus_tag="VUW50_001759"
     CDS             473..1594
                     /gene="mnmA"
                     /locus_tag="VUW50_001759"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET7983612.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVVRDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDEAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1735..2190
                     /locus_tag="VUW50_001760"
     CDS             1735..2190
                     /locus_tag="VUW50_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7983613.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELGVKAQAGQLAFVVENRFEQDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            2200..4113
                     /gene="mnmG"
                     /locus_tag="VUW50_001761"
     CDS             2200..4113
                     /gene="mnmG"
                     /locus_tag="VUW50_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET7983614.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQDKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAIDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            4302..4391
                     /locus_tag="VUW50_001762"
                     /pseudo
     CDS             4302..4391
                     /locus_tag="VUW50_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4493..6172)
                     /locus_tag="VUW50_001763"
     CDS             complement(4493..6172)
                     /locus_tag="VUW50_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET7983615.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(6174..6407)
                     /locus_tag="VUW50_001764"
     CDS             complement(6174..6407)
                     /locus_tag="VUW50_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HET7983616.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            complement(7225..7377)
                     /gene="cibB"
                     /locus_tag="VUW50_001765"
     CDS             complement(7225..7377)
                     /gene="cibB"
                     /locus_tag="VUW50_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HET7983617.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(7379..7564)
                     /gene="cibA"
                     /locus_tag="VUW50_001766"
     CDS             complement(7379..7564)
                     /gene="cibA"
                     /locus_tag="VUW50_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HET7983618.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVILGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            7742..8425
                     /gene="tsaB"
                     /locus_tag="VUW50_001767"
     CDS             7742..8425
                     /gene="tsaB"
                     /locus_tag="VUW50_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET7983619.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQILAEMTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPY
                     QQEGLFVSLMDARRNNVYAGFYENAKPVMPEAHLSFEEVLEKVKGASQVTFVGEVGPF
                     VEQIQEHLPRTNYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            8422..8859
                     /gene="rimI"
                     /locus_tag="VUW50_001768"
     CDS             8422..8859
                     /gene="rimI"
                     /locus_tag="VUW50_001768"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET7983620.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            8849..9859
                     /gene="tsaD"
                     /locus_tag="VUW50_001769"
     CDS             8849..9859
                     /gene="tsaD"
                     /locus_tag="VUW50_001769"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET7983621.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFANLDLNAKPSLAFDT
                     ME"
     gene            complement(9899..11205)
                     /locus_tag="VUW50_001770"
                     /pseudo
     CDS             complement(9899..11205)
                     /locus_tag="VUW50_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11597..11677)
                     /locus_tag="VUW50_001771"
     CDS             complement(11597..11677)
                     /locus_tag="VUW50_001771"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7983622.1"
                     /translation="MLPLPTYSPEYNPIEKIWAYIKNISE"
     gene            complement(11878..12204)
                     /locus_tag="VUW50_001772"
     CDS             complement(11878..12204)
                     /locus_tag="VUW50_001772"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983623.1"
                     /translation="MEKVDRDKLKIYIETHPDAYLTEIAAEFNCPPTTIHYALKAMGY
                     SLKKSCTYCEQDPEKVNRFLKELNHLSYLTPIYIYETGVKTYFYLEYDRALSRQLVSL
                     EEDIII"
     gene            12441..13645
                     /locus_tag="VUW50_001773"
                     /pseudo
     CDS             12441..13645
                     /locus_tag="VUW50_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            13638..14434
                     /locus_tag="VUW50_001774"
                     /pseudo
     CDS             13638..14434
                     /locus_tag="VUW50_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375706.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT         4303 a         2673 c         3292 g         4229 t
ORIGIN      
        1 ggctcaggtt cccttgcagt caacacaact gtagacgggt atactgtcaa tgaaaacggt
       61 gaatgggttt aaaccgatta aattaaatca tgttaagaac atttgacatt ttaattttga
      121 aacaaagata aggttcgatt gaatagattt atgttcgtat tctttaggta cctgtcttat
      181 gatttcagga aatgtcatta aaaaaacgac tccttttctc taacttgaaa aatagattag
      241 agaaaatggg ttgttttatt tattatagtt atttgaatga agataagaag aaggtatact
      301 cacgtcattc acataatctg tatattgact ataagtttta aaaaacaatt tttaagctct
      361 tccttgtctt ctctaaccaa gcgtgttata atgaatactg ctcaagcgac cttcaatcgt
      421 gaagcacaca cgaccttcaa tcgtgaataa acgaatagat gggagactta ccatgagtga
      481 taactctaaa acacgtgttg ttgtggggat gagtggtggt gttgattcgt cggtgacggc
      541 tcttttgctc aaggagcagg gctacgatgt gatcggtatc ttcatgaaga actgggatga
      601 cacagatgaa aacggcgtct gtacggcgac cgaagattac aaggatgtgg ttgcggtggc
      661 agaccagatt ggcattccct actactctgt caattttgaa aaagagtact gggaccgcgt
      721 ttttgagtat ttcctagcgg aataccgtgc agggcgcacg ccaaatccgg acgttatgtg
      781 caacaaggaa atcaagttca aggccttttt ggactatgct atgaccttgg gggcagacta
      841 tgtagcgact gggcattatg ctcgagtggt gcgtgatgag gatggtaccg ttcacatgct
      901 tcgtggcgtg gacaatggca aggatcagac ctatttcctc agccaacttt cgcaagaaca
      961 acttcaaaaa accatgttcc cactaggaca tttggaaaag cctgaagttc gaaaactagc
     1021 agaagaagca ggtctttcga ctgctaagaa gaaagactcg acagggattt gctttatcgg
     1081 agaaaagaac tttaaaaact ttctcagcaa ctacctgcca gctcagcctg gtcgcatgat
     1141 gactgtggat ggtcgcgata tgggcgagca tgcaggtctt atgtactata caatcggtca
     1201 gcgtggcgga ctcggtatcg gtgggcaaca cggtggtgac aatgcccctt ggttcgttgt
     1261 cggaaaagat ctaagcaaga atattctcta tgtaggacaa ggattctacc atgattcgct
     1321 catgtcaacc agcctagagg ctagccaagt ccactttact cgtgaaatgc cagaagagtt
     1381 tacgctagaa tgtacggcta aattccgtta ccgtcagcct gactctaagg tgacagtcca
     1441 tgtcaaagga gacgaggcag aggtcatctt tgcggaacca caacgcgcga ttacaccagg
     1501 acaggcagtt gtcttttacg atggcgaaga gtgtctaggt ggcggtttga ttgacaatgc
     1561 ttaccgcgat ggacaagttt gtcagtacat ttagattgac aaattttctc aatttgctac
     1621 aataataaca gcaatagaaa tgatggtcaa agctcatgga tgttgcaggc ttttttgtcc
     1681 tgcacttctt tgtagttttg actgtttttg tgtcgtttaa gggaaaggac aagaatgact
     1741 caacaagact ttcggacaaa agtagacaat acggtttttg gtgttcgggc tacagccttg
     1801 attgtccaaa atcacaagct tctagttacc aaagacaagg gcaagtatta cactattgga
     1861 ggtgcgattc aagtcaatga aagcacggaa gacgcggtag tccgtgaagt gaaggaagaa
     1921 ctgggtgtca aagctcaagc tgggcagcta gcttttgtgg ttgaaaatcg ttttgaacaa
     1981 gacggcgttt cctatcacaa tatcgagttt cattatctgg tggatttgct tgaagatgcc
     2041 ccgttgacca tgcaggaaga tgagaaaagg cagccctgtg agtggattga cttggataag
     2101 cttgaggata tccagctagt tccagccttt ttaaaaacgg ccctaccaga ttgggaaggc
     2161 caactaagac acattcatct tgaggaatag gagagaaaca tgacttatca ttttactgaa
     2221 gaatacgata ttattgtaat tggtgcggga cacgctgggg ttgaggcttc cttggccgct
     2281 agccgtatgg gctgtaaggt cctgcttgcg accatcaata ttgaaatgct ggctttcatg
     2341 ccttgtaatc cctctatcgg tggttctgcc aaggggattg tcgtgcgtga agtcgatgcc
     2401 ctcggtggcg agatggccaa gactattgac aagacttaca tccagatgaa gatgctcaac
     2461 acagggaagg ggccagctgt ccgtgctctt cgtgcgcagg ctgacaagga actttactct
     2521 aaggagatgc gcaagacagt tgaaaatcaa gaaaatctga cccttcgtca aaccatgatt
     2581 gatgagattt tggtggaaga tggcaaggtt gtcggtgtgc gtacagccac ccatcaagaa
     2641 tatgctgcta aggctgttat tgtgacgaca gggactgctc tccgtgggga aattatcatc
     2701 ggagacctca agtactcatc aggtcctaac cacagcttgg cttctattaa cctagctgac
     2761 aatctcaagg aactgggcct cgaaatcggt cgtttcaaga caggaacccc tccacgtgtc
     2821 aaggcctctt ctatcaacta cgacgtgaca gaaatccaac caggagatga agcacctaat
     2881 cacttctcat acacttcacg tgatgaggat tatgtcaagg accaagtgcc atgctggttg
     2941 acctacacca atggtaccag tcatgagatt atccaaaaca acctccaccg tgcgcctatg
     3001 tttacaggtg tggtcaaggg agtggggcct cgttactgtc cgtcgattga agacaagatt
     3061 gtgcgctttg cggacaagga acgtcaccaa ctcttccttg agccagaagg gcgcaatact
     3121 gaggaagtct atgtgcaagg actttcaacc agtctgcctg aggatgtcca gcgtgacttg
     3181 gttcattcca tcaaaggttt ggaaaatgca gagatgatgc ggacaggtta tgctattgag
     3241 tatgatatgg tcttgcctca tcagttgcgt gcgactttgg aaaccaagaa aatctcagga
     3301 cttttcactg ctggtcagac aaatggaaca tcaggttacg aagaggcagc aggccaaggg
     3361 attatcgcag ggatcaatgc ggctctgaaa atccaagata agcctgagtt gattttgaag
     3421 cgtagtgacg gttacattgg ggtcatgatt gatgatttgg tgaccaaggg aaccattgaa
     3481 ccctaccgtc tcttgaccag tcgtgctgaa taccgtctca ttcttcgtca tgacaatgct
     3541 gatatgcgct tgactgagat gggacgcgag attggccttg tggacgatga acgctgggct
     3601 cgttttgaaa tcaagaaaaa tcaatttgat aatgagatga agcgtctaga cagtatcaaa
     3661 ctcaagccag tcaaggaaac caatgctaag gttgaggaaa tgggcttcaa gccgttgacc
     3721 gatgcagtga cagccaagga attccttcgc cgtccagaag tttcttacca agatgtggtg
     3781 gccttcatcg gaccagctgc agaagacttg gatgacaaga ttatcgaatt gattgaaaca
     3841 gaaatcaagt acgaaggcta tatttccaaa gccatagatc aggttgccaa gatgaaacgt
     3901 atggaagaaa aacgcattcc agccaatatc gactgggatg acatcgattc tattgcgacg
     3961 gaagctcgtc agaagttcaa actcatcaat ccagaaacca tcggtcaagc cagccgtatt
     4021 tcgggagtaa acccagcaga tatttctatt ttgatggtgt atcttgaagg taaaaatcgt
     4081 agtatttcta aaactcttca aaaatcaaaa tgatacgtcg tcggcttctt acgaatgagt
     4141 tcaaagcttg gctttgattc atctccaacc tcccatagtt ccccgaacta tgggagctaa
     4201 ctcaagttat gcattttcct agccttctgg tctgattttc attgagtttt aatctcaaaa
     4261 gaaagcatag agaaaaacag ctccgaagac ggggctgttt tgttgacttg tactcaatga
     4321 aaatcaaaga gcaaactagg aagctagccg taggttgctc aaagcactgc tttgaggttg
     4381 tagatagaac tgacgaagtc agctcaaaac accgttttga ggttgtggat agaactgacg
     4441 aagtcagtaa ccatatatac ggcaaggcga agctgacgtg gtttgaagag tattattctt
     4501 catctggtgt gaggatcatc gggatgatga tcggttcacg ttcggtattt tcatagagga
     4561 aggggcgaat agcgttgaca atggcaccat tgacagattg cacgctagca tccttatttt
     4621 tcagtgcgat acgaatggca ttgaagagga tacgctggct ttggcgaatc aagtcgccag
     4681 actctctcat gtagacaaag cctcggctga ggatgtctgg accagataga atcatctgcg
     4741 atttgaagtc aacagttgcg actgccagaa cgacaccgtc ttcagataga tcgcgacgat
     4801 ctttgaggac agctgcgcca atttcaccga tacgatttcc atcgacatag atatcttggg
     4861 cgttgaaatg acctgcgata cgagctgagt cagcagtaag ggcaagcaca tcgccattgc
     4921 tcatgataaa gatattgtcc ttctcaacac cagtatccac tgctagtcca gcgtggactt
     4981 tttgcatgcg gtattcaccg tggacaggca tgaagtattt tggcttaatc aagcagagca
     5041 tgagtttttg ctcttgctga ccaccgtgtc cagatgtatg gatattgttc actttaccgt
     5101 ggataacttc gacaccagct tcagaaatga tgttaatcag cttgttgacg ctagtagtgt
     5161 ttccagggat gggactagaa gagaagataa cggtatcacc tggttgtaat tgtacttgac
     5221 ggtgggttcc gttggcgata cgagagaggg ctgccatagg ctcaccctga ctacctgtac
     5281 agaggataag aacttctcct gcaggataat ctttgatttc atttggctcg ataaaggttc
     5341 ccttaggagc tttgatgtag ccaagatcga ttccgttgac aatggccttt tccatagaac
     5401 gaccaaagac cgcaattttg cgtccagtct taacagcagc ttctgttgcc tgctggagac
     5461 ggaagatatt tgaggcaaag gatgcaaaga tgatacgtcc ttcaatacct tggataatct
     5521 tcataatgga ctgaccaacg actttttcag agttggtaaa ggttggtact tccgcatttg
     5581 tcgagtcaga caggagacag agcacgcctt cttcaccaag cgcagccata cgatgcaagt
     5641 ccgcaggttc tccaactgga gtaaagtcga acttaaagtc acccgtacag acgattttcc
     5701 cttgaggagt atgaatgaca atccccaaag gctctggaat agagtgagtc gttctaaaga
     5761 aagttgcctt gagattttta aaggtcaact cggtgttgtg gttgatttcg taaagtttgg
     5821 cgttgcgcaa gaggccgtgt tcttcgagtt tcccacggat caaagccaag gcaagcggtc
     5881 cagcataaat agggacattt gcttgcttga gtaggaacgg aatcccacca atgtggtcct
     5941 cgtgtccgtg tgtgattaaa acagccttga cgcggtcgat attgtctacg atgtaagagt
     6001 agtcaggaat gacatagtcg ataccaagca agtcatcttc tgggaattta atcccagcat
     6061 cgacgatgat aatctcgtct tggtattcaa ttccgtaagt gtttttcccg atttctccta
     6121 gaccaccgat ggcaaaaacg ccgacttctt caggtttaag agtgtaggcc atattagaac
     6181 tccgtaattt cgaaggcgcc agtttctttt tcgtaatcga gcaatttgtc agacaagagt
     6241 tcgatatatt cgatattgta ctctgggcga ttttcttcga caagttggcg agcagtgata
     6301 cggccctcaa gttctgagct ggcatcgatg tctaggtaaa gcgcgcgtgt tgtttcacgg
     6361 cgtgggctac gttcttttgt ttcttgataa aaaactttgt aaatcatata gttcctttct
     6421 atttacaggt cagcttattc caaactttta gcagatattt gcttgatttc attccatttt
     6481 gtgtctagat tgaccttgat tcgttacaat tatttcaatt ataccacaaa atgaaaaatt
     6541 agtaaaagac ggaaacgaga aagttagggg acgatttctt cggtaagcgt ttgcaaaaat
     6601 aaagaaaatg tgttatgttg ataacggaag tgaagtttat tacttgtcat aattccatat
     6661 agattttaag tcaaggaggt tgccctatgt ataacttatt tgcttttgct atcggagttc
     6721 gtttgttagc ttttatcgtt tttgttgctt gcgtttatgg aggaaatctt ctatttgcca
     6781 aactggaacg atgacaaacc aagttcttgt ggaaaattac ctttgtgact ctctactcgc
     6841 tttttctcct cctcgtgacc tatgtcgttt ggtttgtatt ttactttgga ttaaatgctt
     6901 agatgccctg cttatgctgg ggttttttgt ttggggatgt agagatagta gatttacgac
     6961 acgaaaattc caccccatct atcaagatga ggtggtcttt gcatttttct aacatgacat
     7021 gacaatggaa ttttcaaagc ttattatttt tatgggagtc attgttctgc tcaaaaatag
     7081 agttgaagca taaaaataaa acaatacaaa cagataaatc gaatggtaaa gggatagtag
     7141 taatgttcaa atcattgaga atgctgagca aactgtaaga gatgaagaca ataaaattta
     7201 tccttctaaa aatgtgtttc ttatctagca tgacttacca aactttttac gaaggtcttt
     7261 tccaatggta taacctgcca taaataaagc gataccaaca cctacaccaa agccaccctt
     7321 gatttcttgc aattccttat tagaaatttc acgatagttg ttcaaatctt tcatcatatt
     7381 aatctcctcc taaaaaataa ccagctgcca aaccagaacc tataagtgct gttccacctg
     7441 caatagcctt ccaagatact gctactccca agataaccaa gggagcgaga ccgccatcaa
     7501 tatctgacaa ttcattttca gataaggata aaaattgatt gtcaagaatg tcaaaatttg
     7561 tcattgaact ttcctccttt tttcttgttc acttttatat tcggaaaaga aaaggaaaag
     7621 tgagcatttt tttaaattat ttttagttaa gtgagggcac tcttgtctgg gggcaaagaa
     7681 aattcccatg tgccagcttt tgtagtataa tagtaagcag acaaaaagat aggaatgtgt
     7741 tatgaaagta ttagcttttg atacgtccag caaggctctt tctctcgcta ttttagagga
     7801 caagcagatt cttgccgaga tgacgattaa tattaagaaa aatcacagta ttaccctcat
     7861 gcctgccatc gattttttga tggcaagttt ggactggaca cccaaggatt tggaccgaat
     7921 cgtggtggct gaagggccag gtagctacac aggcttgcga attgcggtag ccactgccaa
     7981 gactttggct catactctga acatcgagtt agttggtatg tctagcctct tggctctggt
     8041 accctaccaa caagaaggct tgtttgtctc cttgatggat gcgcgtcgca acaatgttta
     8101 tgcaggattt tatgaaaatg ctaaacctgt catgccagaa gcgcacctat cttttgaaga
     8161 ggtgctagaa aaagtcaagg gtgctagtca ggtaaccttt gtcggagaag ttggcccctt
     8221 tgtggagcag attcaagaac acttgccaag gactaattat aaagaaacct tgcctaatgc
     8281 agccaatctt gctcttttag cttgggacaa ggaagcagac tccttgcatg attttgtgcc
     8341 gaattacctc aaacgagtcg aggctgagga aaactggctc aagaaccata ccgagtctgg
     8401 cgagtcttac attaaacgcc tatgatagaa atcaagcgaa ttcaacaaca gcctgaccta
     8461 gctcaagcca tctacgctgt tatggcagct gtttacctag tcagtccttg gactctggag
     8521 caaatccaag cagatctgtc ccaagaccag acttggtatg cattggctta tgatggggca
     8581 gaagtgattg gatttctagc tgtgcaggag aatctttttg aagcagaagt cctgcaaatc
     8641 gctgtcaaag gagcttatca gggtcaggga attgcgtcag ccttgtttgc tcaattgccg
     8701 acagacaagg aaattttcct cgaagtcaga cagtcaaatc aacgagcgca agcattttac
     8761 aagaaagaaa agatggcagt tatcgctgag cgaaaggcct actaccatga cccagtcgag
     8821 gacgccatta tcatgaagag agaaatagat gaaggataga tatattttag catttgagac
     8881 atcctgtgat gagaccagtg tcgccgtctt gaaaaacgac gatgagctct tgtccaatgt
     8941 cattgctagt caaattgaga gtcacaaacg ttttggtggc gtagtgcccg aagtagccag
     9001 tcgtcaccat gtcgaggtca ttacagcctg tatcgaggag gcattggcag aagcagggat
     9061 taccgaagag gacgtgacag ctgttgcggt tacctacgga ccaggcttgg tcggagcctt
     9121 gctagttggt ttgtcagctg ccaaggcctt tgcttgggct cacggacttc cactgattcc
     9181 tgttaatcac atggctgggc acctcatggc agctcagagt gtggagcctt tggagtttcc
     9241 cttgctagcc cttttagtca gtggtgggca cacagagttg gtctatgttt ctgaggctgg
     9301 cgattacaag attgttgggg agacacgaga cgatgcagtt ggggaggctt atgacaaggt
     9361 cggtcgtgtc atgggcttga cctatcctgc aggtcgtgag attgacgagc tggctcatca
     9421 ggggcaggat atttatgatt tcccccgtgc catgattaag gaagataatc tggagttctc
     9481 cttctcaggt ttgaaatctg cctttatcaa tcttcatcac aatgccgagc aaaagggaga
     9541 aagcctgtct acagaagatt tgtgtgcttc cttccaagca gcagttatgg acattctcat
     9601 ggcaaaaacc aagaaggctt tggagaaata tcctgttaaa accctagttg tggcaggtgg
     9661 tgtggcagcc aataaaggtc tcagagaacg cctagcagcc gaaatcacag atgtcaaggt
     9721 tatcatcccc cctctgcgac tctgcggaga caatgcaggt atgattgcct atgccagcgt
     9781 cagcgagtgg aacaaagaaa actttgcaaa cttggacctc aatgccaaac caagtcttgc
     9841 ctttgatacc atggaataaa gagtggctct ttgtcaagtg tagtgggtag gcgaaaagct
     9901 acaatctgga gagtacgaaa ttcgtactct cttttttgat gttcaaagtc atcaaaatcc
     9961 gttttttaaa attgttaaag ttccggaaac caaaggcttt gcgcttgata accttaatca
    10021 aattattggt cgcttcaagt ttagcgttgg aataaggcag cttaagtgcg ttgataatct
    10081 tatctctatc ccttaaaaaa gtccaaaaga ccgtttgaaa aagcggatga accgttggca
    10141 gttcttgctc aatcaatcca aagaaatggt cggcattctt ctcttgaaaa tgaaagagca
    10201 ggagttgata gagttcgtag taaacctgta atccctcaga gtaagaaagc agcttatctc
    10261 gtacttcccg atgggttaag tgcattcgaa acgtgggctg atagaatcgt ttctgacaga
    10321 gcttgaagct atcctggtgt agtaaatcag tccagtggac tgatttagcc cgagcctaga
    10381 aacgaaaaag cgagggttcc aaaagcgctt gagcgcccga tactccaagg attttcggtc
    10441 aaattggttc atgatttgga ttcgtactcg gttcatagct cggctcagat gttggacaat
    10501 gtggaagcgg tcaagaacaa tcttcgcctt tggaaagagt tgcttagcga gccgataata
    10561 agggctgtac atgtccatgg tgatgacctc gaccagctcc cgaacctctc tctgatagcg
    10621 ttggaaatgg tttcgaatca ccgcatgagt tcgcccgtct aaaattgcga ggatggattt
    10681 ggactcaaaa tcttgggcaa tgaagctcat tttgctcttt ttgaagctat actcatccca
    10741 actcataact tttggcaact tggtccaatc ggtttcaaac ttaaattccc tcagttttcg
    10801 gatgacggtg gaagttgaga ccgccaatct gtgggcaatc tcagtcatag actgcttttc
    10861 aaggaggagc tgagcgattt tttgtctcac catgttggaa atctggcaat ttttcttgac
    10921 aatggatgtt tgagaaacga ccaccttaag gcaattcttg cactgaaagc ggcgcttttt
    10981 gagatgcagt accgtgggta aaccctgaca gtcgagaagc ggaattttag aggctttctg
    11041 gaagtcgtat ttgatcatct tcccttgaca atgaggacaa ggaggagcgg gggaatccaa
    11101 ctttgcctga acgactagat gggtttggta tttcagaaca gacaagattt tgatattttt
    11161 gtcttccaat ccgagcaaat ctgtggtatt cttaagttgt tccatatgaa ccctttctaa
    11221 tgagttggtt agacgctttt cattataggt catatggggc tttttttcta caagaaacga
    11281 ccctataatt cctggggtgg gattacccac tacagaaatt atagagccaa agcattccaa
    11341 agtcttgtct gactctggaa tgttttctgt tcacagttgt tttgtaactg cctaataagt
    11401 aaccgcgcta taacgaggta tctaccgaga ttgtcaattc tccagtatac tgttataaaa
    11461 aacaaaactg gaggattttt ttatgtcccc ccatcgtccc atcgactgtt ccgtagctgc
    11521 ccaataacgg agtatagtcg gctgaaacaa gaataggaca aaagtgcctc aagaaaagca
    11581 tcgtaatttg acaatattat tctgagatgt ttttgatgta agcccatatt ttctcaatgg
    11641 gattatactc gggtgaatag gtaggaagtg gtaacagtct atgccctgct ccttacacat
    11701 gttcattctg tgaaaccttg cattgtccat aatgataagg gatggtgtat ctaaagtagg
    11761 tagtaagaat ttttggaagc aagcttcgaa ggagccatag tatattgaaa caaaaaaggg
    11821 cattagtact tctataacag tatcgcaatc tctggtgtgc gttgtctcga tcttaattca
    11881 aataattata tcttcttcca gagagactaa ctgcctgctc aaggctcgat catattcgag
    11941 ataaaaatag gtcttaaccc ctgtctcata aatataaata ggagtcaggt agcttaagtg
    12001 attcaattct ttaaggaacc gatttacttt ttctgggtct tgttcgcagt aggtacagct
    12061 cttttttaga ctatatccca tagcctttag agcgtaatga atagttgttg gaggacagtt
    12121 gaattcagca gctatttcag tcaaataagc atcgggatga gtttcaatat agatctttaa
    12181 tttatctcta tcaacttttt caattaatag tggaagtttt cttaaagtta gtgtattgaa
    12241 acgtttttat acagtgctaa aatttgaagt gtagtgcaga tgtatttgta atagtattat
    12301 agcaattctt tgaagaaggt cgagacaaga gaaaataggt atctttgaga tataagcatg
    12361 atttactata aaattattga gttattcata aaaatttctc tatcaaatat cataatgcgt
    12421 cgtttaagga gatggttaca atgcaatata gttgtggaaa aataaatatt aatatccctg
    12481 atggatatgg agacattaag gatatagtgt tttcggctca tattatagtt aggtataata
    12541 atggacattg tggaggtata gatccgcata taattggact ttgtaaaaag cagatcagaa
    12601 ggatgtctct atatcctatt ttgataatcg tatctagaga ttcaaaggtt attgatgatt
    12661 ataaaaattt agatattgcc tatgttgatt gtactcaatg ttcaaataat tttgaaactg
    12721 ccttacacgt taaaaatatt ttaaaattat taaaaatcca acttatacac tgtcatggct
    12781 actcaactaa ttatttttta tatatgttaa aaaaacttga caagaatgga tttggaaagg
    12841 ttaagacagt gataacatgc catggatggg ttgaatataa cttaaaaaag aaattcttga
    12901 cttattttga tttttggaca tattctatgg gagatgcttt tatttgtgta tctgaaacta
    12961 tgaaaaaaga ttggagagta taataaaaaa taagaagata attgcaataa ataacggaat
    13021 taatgtatca aatagcgatt tagatgtagt aggtgttcaa gactttaaaa aagaattttg
    13081 tattccaaat aacaaaaaaa tcatttgtta tattggaagg ttggatccag aaaaaaggcc
    13141 ggatagattc cttgaatttg cagaaaaatt atttttagtc agagaagatg tgatttttat
    13201 tatggctgga aatggtagta tgtgggctgc tttgaaagaa aaaatttgcc atttgaaatg
    13261 tagagataat ttcaggttac ttggtgaaat ctatccagct actatagttt accaaatatc
    13321 ggatttattg tatattcctt ctgatacaga aggaatacct atgtgtgttt tagagtcaat
    13381 gtcacaagga actccggttt tagctagtaa tgttggcggg ttaagtgaaa ttattgaaca
    13441 tagggttgat ggatttttat ttgagaagga agatgttgag ggagtgtgtg cttgtgctaa
    13501 ttttttactc aatgattctg agtatttgaa atatataggt gagaatagta aatcaaaaat
    13561 aagaaaacat ttttctgtgc aaaaaatgtt tgtagaaacc atgagagtat atgatgaatt
    13621 attagagaag agtagtcatg gataggaata tagatcaaga attagtaagt attataatac
    13681 caacacataa taggtatgaa tctctgatta gagctgtgaa aagttgttta catcaaagtt
    13741 ataaaaatat cgaagttata attattgatg ataattatag taatgtaaat ttaagaaata
    13801 aaattatcca tcaatttggc tataccaatc atagaattaa gttaatttta agtaatgaag
    13861 atttaggtgc aactaatgcc agaaacatag gtatcaaaaa ttctagaggt aagtatatat
    13921 catttttaga cgatgatgat gaatatatgt cagatcgaat tttaaagttg atggcttgtt
    13981 ttaaaaagag tagaatgaag aatttagctt tagtttatag ttatggaata ataatttatc
    14041 caaatggtac acgagaagag gagaagaccg attttgttgg aaatcccttg tttgttcaaa
    14101 tggttcacat caggacggtg ttgttttatt aaaactactt gctcaaggat accaaattga
    14161 tatagtgcga gaattcttgg tgaattacta cgctcacagt aaagaaaacg gtatcactgg
    14221 agtgacacaa aaaacaatta atgcagatga agaatattat aattactgta ggaaatattt
    14281 taatttattg agtttcaacg agagaatatt ggttacaaag aaatattatt ctttaaacat
    14341 aaagcggtta ctattaatag gagacaaatg caaggcttta aaagtaatca agaaggcaag
    14401 agaagaaaaa atttttaacg aatttctttt ttgaaatata tgttattata tcattttggt
    14461 tttttctatt gtatatatga caactatgtt caattaa
//
LOCUS       DAVDWT010000042        14265 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVDWT010000042 DAVDWT010000000
VERSION     DAVDWT010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14265
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.042"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(482..707)
                     /locus_tag="VUW50_001775"
                     /pseudo
     CDS             complement(482..707)
                     /locus_tag="VUW50_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1555..2427
                     /locus_tag="VUW50_001776"
     CDS             1555..2427
                     /locus_tag="VUW50_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HET7983624.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDTFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTESTTDVKVHAEKMEAFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(2573..2917)
                     /locus_tag="VUW50_001777"
                     /pseudo
     CDS             complement(2573..2917)
                     /locus_tag="VUW50_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            3060..3371
                     /locus_tag="VUW50_001778"
     CDS             3060..3371
                     /locus_tag="VUW50_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983625.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKKREELRQIITTIFEQKMNKHSI
                     K"
     gene            3375..4091
                     /locus_tag="VUW50_001779"
     CDS             3375..4091
                     /locus_tag="VUW50_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7983626.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVSDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(4220..5035)
                     /locus_tag="VUW50_001780"
     CDS             complement(4220..5035)
                     /locus_tag="VUW50_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HET7983627.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKITSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(5032..5937)
                     /locus_tag="VUW50_001781"
     CDS             complement(5032..5937)
                     /locus_tag="VUW50_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET7983628.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            complement(6267..6350)
                     /locus_tag="VUW50_001782"
                     /pseudo
     CDS             complement(6267..6350)
                     /locus_tag="VUW50_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            6394..8523
                     /locus_tag="VUW50_001783"
     CDS             6394..8523
                     /locus_tag="VUW50_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HET7983629.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            8510..8959
                     /locus_tag="VUW50_001784"
     CDS             8510..8959
                     /locus_tag="VUW50_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HET7983630.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLT
                     NSHSKPLKLYRCQSCQQTYQRKRRIDTKRYRCGLCRGKLLLVNQPED"
     gene            9018..9290
                     /locus_tag="VUW50_001785"
     CDS             9018..9290
                     /locus_tag="VUW50_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET7983631.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(9644..9835)
                     /locus_tag="VUW50_001786"
     CDS             complement(9644..9835)
                     /locus_tag="VUW50_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983632.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            10263..11021
                     /locus_tag="VUW50_001787"
     CDS             10263..11021
                     /locus_tag="VUW50_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7983633.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            11023..13011
                     /locus_tag="VUW50_001788"
     CDS             11023..13011
                     /locus_tag="VUW50_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7983634.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMNVVIAVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKRGRFLPLQTILGSISLGIGYYLAL
                     TVTDPLTALTTFFLAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPTTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVNVSEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(13053..13697)
                     /locus_tag="VUW50_001789"
     CDS             complement(13053..13697)
                     /locus_tag="VUW50_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HET7983635.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATTNIWIIFLSGFTAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLGQLFSI"
     gene            complement(13723..>14265)
                     /locus_tag="VUW50_001790"
     CDS             complement(13723..>14265)
                     /locus_tag="VUW50_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET7983636.1"
                     /translation="STNFKPAGQSIEQRLEAINFRLENGHYEIDTVLLTRAKNYCLLV
                     LTDRKSRHQIIRLIPNKSADVVNQALKLILKQHKILSITADNGTEFNRLSNVFSEEHI
                     YYAHPYASWERGTNENHNRLIRRWLPKGTKKMTPKEVAFIEKWINNYPKKCLDYKSPR
                     EDFLMANLNLKFSVRNKSRN"
BASE COUNT         4331 a         2640 c         2942 g         4352 t
ORIGIN      
        1 aaataagata tgcacaaatt gattagaaag tcaaatgaat ttctacaaat gttttagcaa
       61 tcgcaatcta cttgtctaga ttcgatctga tatattttcg atttaatgat atggtattta
      121 aaacctccaa agtagcttgc tccattcttt tacttacgtg agtgtagatg ttatttactg
      181 ttttagcgtt tttgtgttcc actctaacca ttatagcatt cttctcagct agtgtactaa
      241 ggagtgtgtg cctgaaaata tgggaactaa ggggctggtt tatcggtttc tctagtttag
      301 tatttgcctt ttgcaaagtg atgttaaatg cctttctcta aatttacata tcactattgt
      361 ttaacaaaat ctaatctact ttaggtcact tattcttttt tgcaatgtag aatgaacttt
      421 ttcaaagttt ttcgaatctt ttaaaatctg tttgttttat atcgccattc tccccccttt
      481 tttaattctc cctatatagc ctgacaactt tcccgatggt acaaatatgg ttgctttcgt
      541 ctaggtggat gtcggggtat tcgggattga gtttttttga ggtagccttg gcggagtttc
      601 ttgacatagt tagcgccgtc tacttggaag atgccgatgg tattatagtc aatctgtggg
      661 gtattcttga taaataggta gtcgctgttt cttatctttg gctccatgga cttgctgacg
      721 acataagcga ttgggtcgta gtcgtctggg ataatggaaa ctccatatct aaatcgttgt
      781 cctgcatcga gcggctacct gcagagataa actacctaac aagagagtaa gtagtctgtc
      841 tgtagtcgtc cagtctgatg atttttacga tacttcgttt ttctgatcat acagttgcct
      901 ctcggcatag gtcagaactt taccttgtct gggtggtgcc cgttggtcgt agatagattg
      961 gatatcgcta ggagaatcct tttgaactgg aggaaagagg gcatcaatca agctactgaa
     1021 tactttaact aagtcaaata tagtattttt cttagtagac ctaacccttt tttcataatt
     1081 tctaatggtg tttttactta tacctatctt agtacccaat tcttattgag tccaaccatt
     1141 actagtctat attgttttat agttgattga gtttggaata gtacgctgta gctgctaaaa
     1201 catttctagg aattaatttg cctttcctaa tagagttgtt catatcttat ttcaatctat
     1261 gatgtttttc acctctaaca atcgcaatct cttctttatc catgaatgaa aacgttttct
     1321 atttttgtaa gtaaagcatc acacgaaatt cacgaaaatg aaaacctttg ttgtgttttc
     1381 gtaaaaaatt tgttgacaga gcacgaaacg cctactataa ttaagtcaag cttaaggaaa
     1441 taacaaaaaa acaaatcgga gggaaagacg atgaacacat taaatgagaa agtaatcaat
     1501 atctgtaaag cagtagttaa agaaacttta atccaagaca tttaggagaa agaaatgaag
     1561 aaatcaagaa aattagccac attaggaatc tgttcagcct tgtttttagg cttggctgct
     1621 tgtcaacaac aacatgctac ttctgagggg acgaatcaaa ggcaaagcag ttcagcgaaa
     1681 gttccatgga aagcttcata caccaaccta aacaaccagg taagtacaga agaggtcaaa
     1741 tctctcttat cagctcactt ggatccaaat agtgttgata cattttttaa tctcgttaat
     1801 gactataata ccattgtcgg ctcaactggc ttatcaggag atttcacttc ctttactcac
     1861 accgaatacg atgttgagaa aatcagtcat ctctggaatc aaaagaaggg cgattttgtt
     1921 gggaccaact gccgtatcaa tagttattgt cttttgaaaa attcagtcac cattccaaag
     1981 cttgaaaaga atgaccagtt gcttttccta gataatgatg cgattgataa aggaaaggtc
     2041 tttgattcac aagataagga agagtttgat attctatttt cgagagttcc aactgagtca
     2101 actacagatg tcaaggttca cgctgaaaag atggaagcat tcttctcaca atttcaattc
     2161 aatgaaaaag ctcgaatgct gtctgtagtc ttgcacgaca atttggatgg cgagtatctg
     2221 tttgtaggcc acgttggggt cttagtacct gctgatgacg gtttcttatt tgtagagaaa
     2281 ttgactttcg aagagcccta ccaagcgatt aaatttgcta gtaaggaaga ttgctacaag
     2341 tatttgggca ccaagtatgc ggattataca ggcgagggac tggctaagcc ttttatcatg
     2401 gataatgata agtgggttaa actttaatag tgttatgata tgacagtaat cttaggattt
     2461 ttatttctat ttttgataag ttggtgggtt atttcaatcg ttaatagcca gaaaccacgc
     2521 aaaaatgaaa catttattgg ctatgtccaa agatacgata ttgatgggaa tggctatcaa
     2581 tcattaccgt gtcctcagaa ctgagaggag ttcttgaaat cgtaacacca ctttgaacaa
     2641 gagttactta aacccattgg ctccgacgga ttaagttgct ttcgtgaata ccaaaatcag
     2701 ccgcaatttg ttcataagtg cggtattctc gcacatattg aagagtggcc ataagaaggt
     2761 cttctaggct taatttaggt tttcgtccac cttttgcgtg tttaagttga taagctgttt
     2821 ttaatacagc taatatctct tcaaaagtcg tgcgctgaac accaacaaga cgtttaaatc
     2881 gtgcatcagt tagttgttta cttgcttcat aattcataga actactatac catattttgt
     2941 ttcgcaggaa gtctaatgct aaaatcttat cagtgagaag atttcttaaa gtttattctt
     3001 tgtagtttca tctgtattag tacataattt aacttagaga aaataggagg actaatcgaa
     3061 tggaattaaa agattttaca gaaaaagaac aggaaatgat taagaaaagg cttacaatgt
     3121 ctaatattag tgacaaagaa actactgaga agattctggc gctagtccca caagacttga
     3181 ttaagcgaat cccgtttttt gtcagaaaac atgctacaac acgtacgatt aaacgcattt
     3241 caattgaata ccctgaactc tacgctgtag ctcaaacaag tggtgagatt ccagaaaaaa
     3301 aacgcgaaga attgcgtcag attatcacaa ctatctttga acaaaagatg aataagcata
     3361 gtattaagta gagaatgaaa aaatatttta ttggcggttt gggaagcaat gcctatcata
     3421 gcaaggattt tcttcaagaa ctagattcgc aggtctattt tctaaatcca tatgaaaagc
     3481 atcttcgaga tgaaacagaa ttgaaatcat ggtttaaaaa tgaaattgta gaggaagaat
     3541 ctatctgtct gataggccat tctcttggag gagatttagc tcgttatttc gcatcggaat
     3601 ttgaagaagt aaagaaactg attcttttgg atggtggcta tctagattta gataagattt
     3661 tacctttgga tacagagtta gaggaaacta aaaattatat caaatctcaa attgtttcgg
     3721 acttagatgt tcttacttct aaagaaaaat ctgaagcaaa gcattggtca gaaaatatgg
     3781 agaaagctgt aagacagtct tatcactgga atgttgagta taatagatat gagttggcta
     3841 taaattatga aaatatagaa gcgatactcc gcctacggag aaaaatacaa gcttttaaga
     3901 gagaagtggg agataccttg tttattagtc ctcgctatcc taatgaagct acatggagag
     3961 aggaagccct aaaagaattg ccagactatt ttgatactat ttttttagaa aactttggcc
     4021 atgagcttta tactcaagca cctaaagaaa tcgctagtct aatgaatgag tggctcgctt
     4081 attttctatg agctacttgt attgctggga atggtataag atgaaaatct acaagtcact
     4141 aattttcata taacaaaaaa cgaacaggct ctttttctgt tattcagaag agaacttgtt
     4201 cgtttttatc ttgattttct taaaaagctc tatatacata gggattttct ggtttatcga
     4261 ctttggtaaa gctgtcgatt tccaaggttg gaaggttttg tttgagttct ttatggtagt
     4321 gattgaccag ttttcctttg gctgttatcc aagtgttgtt ttcatactgc cgggtattgc
     4381 ccttggtcag caatccatag acaccagaat ctgcgataca gtggataatg ccgaatcgga
     4441 acagaaattg actattggca tgactggggt cgttatagac aaagcctgta aactggattg
     4501 tcttgccctc aaactcatct ggatagtcgt agatagcctc catgacttcc atatagtttt
     4561 cattagtgat ctgaatacta tcttgggata agtatttatc cgccgccgtt cgcatttcct
     4621 tttcataggc tgattttgaa aaataagaac tggtatctgg tttcaaatat tggcttgtcg
     4681 tcccttcgct tgtctgaatg gctagatccg ttccttccga tagggggaaa tgataacctt
     4741 tagcagaaac agtctgagaa tccaagctaa cagttgggaa agttaagccg atgacaagtg
     4801 gaatagccag aagagaaata ctcgttatct tggctaatcg gctgttcaga tgactgtggg
     4861 ttttgacttg cttcatccag atatacaatt gaacgatagc caagataaaa gaaagcacca
     4921 tggagatata ggccagatag gaatagtgca tgttgatgta ctggtttagt ttgcccgaca
     4981 gatggaggta aatagtcagt tcaaaatagc cagctaaaac taaaaatcga atcataggat
     5041 aactccaatc aagagagaat agactaagac gacaagagtt acaattgtta tgaagtgact
     5101 gataaatcgt gctttcaagt aatttttcat catgagaata tttttgatat ccagcattgg
     5161 accaatgacg agaaaggcca gaactggtgc caaaccgaaa ctcgagagaa gagaagcacc
     5221 tataaaggca tccgcctcac tacagagcga aagaagaaag gctaaaatca tcaagagcag
     5281 gatggcaaaa agaggggtcg cactgataga ggtcagaatc cgagtcggaa cgtagacctg
     5341 tattatagaa gcaaagaggc agccaaatac caaataacgc cccgtatcaa aaaattcatc
     5401 aatggcctgc acaaagactt gaaaaacttt ttttgcagaa ctcaagtaag aaaaatcatg
     5461 ctcatgacaa gccagacgat tttctttctg aatcggttct tgccagaaaa atcctagaaa
     5521 aattcctagt attacagcca caagaatgga acccagagct cgtaatagtg cgacatggaa
     5581 ggagttgcca aaggcagaat aggtcgcaaa aagaacaatg ggattgataa caggtgctgt
     5641 cacaagaaaa ggaacggccg tgtaacttgg aacctttttt tccagaaaac gattgatgat
     5701 ggggacgatt ccacattcac aagaagggaa aagtataccg acaaaggtcc caaaaaagat
     5761 tctcccccaa cgatttcgag ggagaaaatg ataaaccttg tcaggtgtga tataaacttc
     5821 aatcagccct gagacaatgc ttcctatcag aacgaaggga agggcttcaa tgataatgga
     5881 gagaaaaatg gctccagttt gaagtacact agatgggagt tgttgaaaag cagtcatcta
     5941 gtttttcttt tctgctttga cttttttctc tttgtcatca gggaaagtaa cttgtttcaa
     6001 atcagggagt ttagcaaact cttcaaacat cttgtccaag tcgtctcgtt ttgtaaattt
     6061 gacagccata ggtgtacctc gattctaaat tctacctcta ttatactatt attttgcgga
     6121 aaagggagtt ttttaatttg ttttcgatag ttgatacact tcgaaaatct cttcaaacca
     6181 cgtcagcttc gccttaccgt agtacggtta ctgacttcgt cagttctatc cacaacctca
     6241 aaacagtgtt ttgagctgac ttcgtcagtc ttatccacaa cctcaaaaca gtattttgag
     6301 cagcctgcgg ctagcttcct agtttgcttt ttgattttca ttgagtatga aaaggattgg
     6361 agaaagcctt ccagcttgtg gtataataga tttatggata aaaaatatga aaaaatctct
     6421 caggatttgg gagtgacgtt aaagcaaatt gataccgttc taagtttgac agctgaaggg
     6481 gcgactattc cctttatcgc gcgttatcgc aaggacatga ctggtagtct ggatgaggtg
     6541 gcgattaagg ctattattga tttggataaa agtctgacaa atctcaatga ccgtaaggaa
     6601 gctgtcttag ctaagattca agaacaaggt aagttgacca aggaattgga agaagctatc
     6661 ttagttgccg aaaaattagc agacgttgaa gaactctatc ttccttataa ggaaaagcgt
     6721 cgtaccaagg caaccattgc ccgtgaagct ggactctttc ctcttgctcg tttgattttg
     6781 cagaatatag ttgacttaga gaaagaagct gaaaagttcg tctgtgaagg atttgcgact
     6841 ggcaaggaag ccttgaccgg tgcagttgat attttggtcg aagccttatc ggaagatgtg
     6901 accttgcgtt ctatgactta tcaggaagtg ctgagacact ctaaactcac ttctcaagcc
     6961 aaggatgaaa gtcttgatga aaagcaggtt tttcagattt attatgattt ttcagagaca
     7021 gttggaacta tgcaaggcta tcgtaccttg gctctcaatc gtggggagaa acttggtgtc
     7081 ttgaagatcg gttttgaaca tgcgacggac cgtattcttg ccttctttgc tactcgtttc
     7141 aaggtgaaaa atgcttatat tgatgaagtt gttcagcaat ccgttaagaa aaaggtcttg
     7201 cctgctattg agcgtcgtat tcggacagaa ttaactgaga aagctgaaga gggagctatc
     7261 caactttttt ctgacaatct gcgcaatctc ctcttggttg ctccactgaa agggcgcgtg
     7321 gttcttgggt ttgacccagc ctttcgtaca ggtgccaagc tagctgtcgt ggatgcaaca
     7381 ggaaaaatgc tgacaactca ggttatttat cctgttaaac cagcatcagc tcgtcaaatc
     7441 gaagaagcca agaaagattt agcagattta attggtcaat atggtgtaga gattattgcc
     7501 attggaaatg gaacggccag tcgtgaaagt gaagcttttg tagcggaagt tctgaaagat
     7561 ttccctgaag tcagctatgt tatcgttaat gaaagtggtg cttctgtcta ttctgccagc
     7621 gaacttgctc gtcaggagtt tccagacttg accgttgaaa aacgctctgc catttctatc
     7681 gcccgtcgtt tgcaagatcc tcttgcggaa ttggtcaaaa tcgatcctaa gtcaattggt
     7741 gtcggtcaat accaacacga tgtcagtcag aagaaactat ctgagagtct ggactttgtt
     7801 gtcgatacag tggttaacca agttggtgtc aatgtcaata cagctagccc agctcttctt
     7861 tcacacgtag ctggactcaa taaaaccatt tctgaaaata tcgtcaaata ccgtgaggaa
     7921 gagggaaaaa tcacttcacg ctcccaaatc aagaaagttc ctcgtttggg ggccaaggct
     7981 tttgaacagg ctgctggttt cctccgtatc cctgaaagta gcaatatcct tgataataca
     8041 ggagttcacc cagaaaatta cgctgccgtt aaggaactct tcaaatgctt ggacattaag
     8101 gatctgaatg aagaggccca aagcaaactc aagtcgcttt cagtcaagga aatggcgcaa
     8161 gagttagacc ttggtccaga aactcttaaa gatatcattg cagatcttct caaaccaggt
     8221 cgagatttcc gtgattcctt tgatgctcca gttctccgtc aagatgtcct agatatcaag
     8281 gacttagtgg ttggacaaaa gctagaagga gtggtgcgta acgttgttga tttcggtgct
     8341 tttgttgata tcggaattca tgaggacggt ctcatccata tttcccatat gagtcgcaag
     8401 tttatcaaac accctagtca agtggtggca gttggagatt tggtgagcgt ttgggttaag
     8461 aaaattgata ctgaacgtga aaaagttaat ctgtctctcc tagctccaaa tgaaactgac
     8521 tgattacgtc aagcaggttt cactagaaga cttcggcaga ccttttatcc atcatgtcca
     8581 gtggaatagg cgtctacgtt cgacaggtgg gcgatttttc cccaaagatg ggcatttgga
     8641 ttttaatccc aaggtttatc aggaactggg gttggacgtt tttaggaaaa ttgtccgaca
     8701 tgaactctgt cattatcacc tctattttca agggaaaggc tatcaacaca aggatcggga
     8761 ttttaaggaa ctgttgaaag cagtggatgg attacggttt gtaccatcct tgacaaatag
     8821 ccactctaaa ccactcaaac tttatcgttg ccaatcctgc cagcaaactt atcagcgtaa
     8881 gcgtaggatt gataccaaac gctatcgctg tggactttgt cgaggaaagt tgctattagt
     8941 aaatcagcct gaggactgat gaaagccgag cctatccgtg atatactatg tctaacctag
     9001 cagaaagagg aaatatcatg aacagtaagt tttataaaat gagacgaaat cgcatgattt
     9061 caggagtttt agcaggtctg tcagacaagt ggaactttga tgttaccctt gtacgttttc
     9121 tgtttgccat ttttacagtc gcaaactttg gacttggggt gattatctat atcatcctag
     9181 cttctatcat gccaaccaag gaagaaatcg aagcagaaat gtacggaaca ggcccacgta
     9241 aacgcaaaga agctcaagcc attgacgata atgaaggctg gttttggtga ggttttacaa
     9301 aagatttgga atacgcaaaa agctttaaat caatagtcta aactgcttga tttaaagctt
     9361 ttttactttt atactcaata aaaatcaaag tgcaaactag gaagccagcc gcaggctgta
     9421 atttagtcgg caatgtgaag ttgatatagt ttgcactttg atttttgaat agtattaata
     9481 atatttcgcc agcttcatcc acaaactata gtggattgga actagaatag tacacctcta
     9541 cttctaaaac attgttagaa atcgatttga ctatcctgat cgatttgtcc tgttcttatt
     9601 tcattttatt ataaaagcag taatttaaac tcagtttttt aatctatttt tgctgctgag
     9661 acaatacatt tttggaaaaa tggtggatgc tgataacaac tgataaccag caaataacat
     9721 aagcagtgcc ggtaaattct gtaccgataa agtgtgtgtg ttcaactaag agctgaccga
     9781 tgaatggaga gagcagatag agaagtccga tgagtatatg ttctttgtgt ttcattaaag
     9841 cgcaccttct ttccttatag gttgaacgac ttgcaagtgt taaattgaat ggtatgttat
     9901 ctaggtaacc ttactaaaat gttagttccc gcttcctcct tttcaaagta agccttttca
     9961 cacttatatt ttacaacttt aaaaatagga aatcaaatca ttttctaagt aattttgctt
    10021 atatttcacc caatcttaca aaaatgtaag attaaagtct cttttgtaag gttaggttat
    10081 ggcaagcggt ctttttttga tgtaaactag actcataaag aacaaaggag taacatcatg
    10141 aaacaaatct tacataagaa aactagaaaa ccaagtcaca aagaaattga gcgcgttcag
    10201 ctgggatgca ctatgatgca agctacattt catttgatgg gatattaaga aaggagattc
    10261 tcatgacact tttagatgta aaacacgttc aaaaaattta taaaacacgt tttcagggca
    10321 accaagtaga agccctcaag gatattcact ttaccgtcga aaagggtgac tacgttgcca
    10381 tcatgggtga gtctggttct ggtaaatcaa ctcttctcaa tattctagct atgctggata
    10441 aaccaagtcg tggtcaggtt tacttgaatg gaactgacac cgcaaccatt aaaaattcac
    10501 aggcctctag cttccgtcgt gaaaagttgg gctttgtctt ccaagacttt aacttgctag
    10561 atactctatc tgtaaaggac aatatcttgc ttccgcttgt cttgtcaaga agacctatta
    10621 cagagatgat gaagaaattg gtggtgacag ctgagaatct gggtattaac caattgcaag
    10681 agaagtaccc ttacgagatt tctggtggcc agaaacagcg tgtagcagta gcacgcgcca
    10741 tcatcactga acctgaaatt cttcttgcgg acgaaccgac aggagctctt gactccaagt
    10801 catctgcagc cttacttgat gtctttgatg aaatcaatga gcgcggccaa accattctca
    10861 tggtaaccca ctcaacagca gctgctagca gggccaagcg tgttctcttt atcaaggacg
    10921 gcattcttta caatcaaatc taccgtggag agaagacaga acgtcagatg ttccaagaaa
    10981 tctctgatac cttgactgtc atggcaagcg aggtgaatta gtatgtttcg attaaccaat
    11041 aagttagcgg tatcgaactt gattaaaaac cgcaaactct actatccttt tgcgctggct
    11101 gttctcttgg cagtcactgt cacctatctc ttttactctc taaccttcaa tcccaagatt
    11161 gcggaaatcc gtggaggaac aaccattcag gctacacttg gatttggtat gtttgtcgtc
    11221 acccttgcgt cagccattat cgttctctat gccaatagtt ttgtcatgaa gaaccgttcc
    11281 aaggaactag gaatttatgg catgttgggc ttggagaagc gccatctaat cagtatgacc
    11341 tttaaggagt tagtggtatt tgggattcta actgttggag cgggtatcgg tattggagcc
    11401 ttgtttgaca agttaatttt cgctttcctg ctcaaactaa tgaaactgaa ggttgagctg
    11461 gttgctacct tccaaatgaa tgttgtcatt gcagtacttc ttgtctttgg attgattttc
    11521 ctaggcctca tgttcctgaa tgctcttcga atcgcccgta tgaatgccct ccagctctcg
    11581 cgtgagaaag caagcggaga gaaaagaggt cgcttcctac ctctccaaac tattcttggt
    11641 tccataagtt tagggattgg ctattatctt gcccttacgg taaccgatcc tcttacagcc
    11701 ctaacaactt tcttcctagc tgttttgctg gttatctttg gtacttatct attgtttaat
    11761 gcagggatta cagtcttcct acaaatctta aagaaaaaca agaaatacta ttaccaacct
    11821 aataacctca tatctgtttc caacttgatt ttccgtatga agaaaaatgc ggttggacta
    11881 gcaaccatcg ctattttgtc aacaatggtt ttggtaacca tgtcagcagc gacaagcatt
    11941 ttcaattccg cagaaagctt taaaaaagtt ctaaatcctc atgattttgg ggtttcaggg
    12001 caaaatgttg aaaaagaaga tttggacaaa ctcttgagcc agtttgcaag tgacaaaggt
    12061 tatagtgtca aagagaaaga agtacttcgt tacagtaact ttggtattgc aaatcaagaa
    12121 ggaaccaagt taactatttt tgaaaaagga caaaaccgtg tccaacccac aacagttttc
    12181 atggtatttg accaaaaaga ttatgaaaat atgactggtc aaaaactgtc tctatcagga
    12241 aatgaggtcg gtctctttgc caaaaatgac ggactgaaag gacagaaagc tctaactcta
    12301 aatgatcatc aattttctgt caaagaagaa tttaataaag atttcattgt gaaccatgtt
    12361 ccaaataagt ttaatatctt gactactgat tacaattacc ttgttgttcc tgatttacaa
    12421 gcctttttgg atcaattccc agattcggct atctataatc agttttacgg tggtatgaat
    12481 gtaaatgtca gtgaagaaga acaactcaag gtcgctgagg agtatgaaaa ctacctcaat
    12541 caatttaatg ctcaattaga cacagaaggt agctatgttt atggtagcaa tctagcagat
    12601 gctagttctc agatgagtgc cctctttggt ggtgtcttct ttatcggtat tttcctatcc
    12661 attatcttta tggtcggaac tgttctggtc atctactaca aacaaatttc tgaaggctac
    12721 gaagaccgtg aacgctttat tatcttgcag aaagtcggtt tggaccaaaa gcaaatcaag
    12781 caaaccatca acaaacaggt tttaactgtt ttcttccttc ctttgctctt tgccttcata
    12841 catctcgcct ttgcctacca tatgcttagc ctgattttaa aagtgattgg tgtactggat
    12901 acgactatga tgttgattgt gaccttgtct atctgcgcta tcttcctcat cgcctatgtg
    12961 ctgattttca tgattacttc aagaagttat cgcaagattg tgcaaatgta aaaaagatac
    13021 ctcgacttca aaatcgaggt atttcttgta ttctaaatgc tgaaaagttg tccgagcagg
    13081 aaggtaactc ccatggtcaa gagaccaata gcaaggttcc gaatcatagc tgttttggtt
    13141 ggggcttttc caagtctagc acttgtgtaa ccagtgagaa gaagggccac accgacaata
    13201 aggacggtag cagggatgcg gtaatcactt ggaaaaatgg tcactgacag cattggaggc
    13261 aaacttctaa ggaaaaaggc aacgaagcta gaaatggcag cgtgccaagg attggtaaat
    13321 tcttcatact caatcccata tttttcctct accagagcct tgagtggatt tttaagaaag
    13381 atcttattgg tcaagagttg ggcagaagtt ttgaattctc cattttggat ataagcagca
    13441 tagagggatt ttttggctag ttccctatct tggtctagca agagtttttc tcgcgaaacg
    13501 gcagcttcct cggtatcttt tggagttgaa acggatacat attctccacc agccattgaa
    13561 aaggcaccag ctaagatagc cgtaaaacct gataaaaaga taatccagat attggtcgtg
    13621 gcactggcaa ctccgataac cacaccagca atggaaataa ttccatcgtt agcatcaaga
    13681 acacccgcac gcaggatatt taaacgacct gcaaaatttg aatcaatttc gtgatttgtt
    13741 tctgacgcta aatttcaagt tcaagttagc catcaagaag tcttctctgg gtgacttgta
    13801 gtccaagcat tttttaggat agttgttaat ccacttttcg atgaatgcga cttctttggg
    13861 agtcattttc ttggttccct taggtaacca tctacgaatg agcctgttgt gattctcatt
    13921 agttcccctt tcccaagagg catagggatg tgcataataa atgtgctcct cagaaaatac
    13981 attagacaag cgattgaatt ccgttccatt atctgccgtg atggaaagaa tcttgtgttg
    14041 ttttaagatg agttttagag cctgattgac cacatcagca cttttatttg gaatcaatcg
    14101 gatgatctga tgtctacttt ttcgatccgt caagacaagc aagcagtagt ttttcgctct
    14161 cgtaagtaga actgtatcaa tctcataatg cccattctcc aagcgaaaat tgatagcttc
    14221 aagccgctgt tcgatggatt gaccagcagg tttaaagttg gtgct
//
LOCUS       DAVDWT010000043        14086 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVDWT010000043 DAVDWT010000000
VERSION     DAVDWT010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14086
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.043"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..89
                     /locus_tag="VUW50_001791"
     CDS             <1..89
                     /locus_tag="VUW50_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET7983637.1"
                     /translation="STTYRNHRKHFGLRMNLIAGIINHELGF"
     gene            complement(404..2095)
                     /locus_tag="VUW50_001792"
     CDS             complement(404..2095)
                     /locus_tag="VUW50_001792"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET7983638.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGIGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            2231..2677
                     /gene="argR"
                     /locus_tag="VUW50_001793"
     CDS             2231..2677
                     /gene="argR"
                     /locus_tag="VUW50_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET7983639.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            2728..5262
                     /gene="mutS"
                     /locus_tag="VUW50_001794"
     CDS             2728..5262
                     /gene="mutS"
                     /locus_tag="VUW50_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET7983640.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            5409..7103
                     /gene="patA"
                     /locus_tag="VUW50_001795"
     CDS             5409..7103
                     /gene="patA"
                     /locus_tag="VUW50_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HET7983641.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQDGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(7434..7703)
                     /locus_tag="VUW50_001796"
                     /pseudo
     CDS             complement(7434..7703)
                     /locus_tag="VUW50_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            7895..9661
                     /gene="patB"
                     /locus_tag="VUW50_001797"
     CDS             7895..9661
                     /gene="patB"
                     /locus_tag="VUW50_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HET7983642.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     NQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            9800..10489
                     /locus_tag="VUW50_001798"
     CDS             9800..10489
                     /locus_tag="VUW50_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET7983643.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            10493..10672
                     /locus_tag="VUW50_001799"
     CDS             10493..10672
                     /locus_tag="VUW50_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983644.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            10659..12008
                     /locus_tag="VUW50_001800"
     CDS             10659..12008
                     /locus_tag="VUW50_001800"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET7983645.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVIIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            12132..13592
                     /locus_tag="VUW50_001801"
     CDS             12132..13592
                     /locus_tag="VUW50_001801"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET7983646.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTVFLLGREKSIQHIENMLKEISK"
     gene            13606..14040
                     /locus_tag="VUW50_001802"
     CDS             13606..14040
                     /locus_tag="VUW50_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET7983647.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT         4114 a         2714 c         3171 g         4087 t
ORIGIN      
        1 tttcaacaac ctatcgaaat catcgtaaac acttcggatt acgaatgaat ttgattgctg
       61 gcattatcaa tcatgaacta ggattctagt tttgcaggaa gtctattatt tggttaggtg
      121 aattagtgaa gcgtttaggc aagtgtctct ggttacgacg tcatggactc taaatcgatt
      181 atatttaggg gtcatgacta gtgaagcagt tagctagttc gcatataagc ggctagcgtc
      241 taacaattag gaactttagt tccaataact ttaagattac gacgttttag gacataaatc
      301 gatcatattt atgtcctaaa actagtgaag cgcctagcca aagtccgaat aggatttggc
      361 gttagttact tagattgctt tgcaatcaag taactttggc gatttacatc ttctctggcg
      421 cttctactcc aagcaagcga agggcttctt tgagaacgac tgcggttgcg tagctgaggg
      481 ctagacggct gtcgcgttct gggctttcat ccaagatacg tgtatgtgca tagtatttgt
      541 taaaggattg agccaggcta attgcaaatt tagcaatgat agaaggttca aagttatctg
      601 ccgcacggtt gataatacgt gggaagtctt gaatgagttt aatgatttcc cagctttcag
      661 tatcattcaa gctatagttg ccagctgttt ctggtttgaa atcggctttg cgtaagatag
      721 attggatacg agcgtaggca tattgaacgt aaggtccagt ttcaccctcg aaggatacca
      781 tagcctctag gtcgaagtcg tatccatttg tacggtcggt tttgaggtca tagaatttaa
      841 tggctccaat cccaacagca tgtgctactt ggtctttgtt ttctagttca ggatttttag
      901 cctcgatttg gaccttggca cggctaacag cctctgcaac agtaggctct agcaagatga
      961 cattcccttt acgagtagag agtttcttcc cttcttttgt aaccaaacca aaaggaacgt
     1021 gagtaatgtc gtcactccag tcgtagccca tctcttgcaa gacagctttg agctgtttaa
     1081 agtgggcaga ttgttcttga ccaacgacat agatagattt agcaaattgg tattcgtttt
     1141 tacggtagag ggctgcagcc aagtcacgtg tgatatagag agttgcacca tcagacttct
     1201 tgatgagggc tggatgttca attccatatt tctcaagatt cacaacttgg gcaccttctg
     1261 attcaagaag tagtcctttt tcagaaagaa tgtctacaac tgcatccatc ttatcattgt
     1321 agaaggcttc tccgttatag ctgtcaaatt caaccttcaa ttcattgtaa aggcggttaa
     1381 attccactaa actttcatcg cggaaccatt gccaaagagc gagagcttcc tcatctccat
     1441 tttcaagttt acggaaccat tcgcgcgctt cttcatccaa gctagggtca ttttcagctt
     1501 cagcgttgat gcggacatag agtttaagga gttcatcgat tggatgagct tttacagctt
     1561 cttcgtcgcc ccattttttg taggcaacaa tcaacatccc aaattgttta ccccagtctc
     1621 ccaaatggtt gaccttgacc gtttgataac cgattttttg gaaaatatgt gacaagctat
     1681 ctccgataac agttgaacgc aggtggccaa tagaaaatgg tttagcgata ttcggactag
     1741 acatgtcgat aacaacattt tcttgtttac caatattttg gtcagcatag tgttcttttt
     1801 cagtggtaac agcttgcaat acttgagcag aaatggcaga tttatcaagg aaaaagttaa
     1861 cgtaaggtcc tgttgcgaca actttttcaa aggcttggct gttcattttt tcagccagtt
     1921 cagccgcaat catttgtggt gctttacgtt cgacttttgc aagagaaaaa gcagggaaag
     1981 caatgtctcc catttctgag tttttagggg tttccagtaa ctttaaaata gcctcttggt
     2041 ccaggctatc aatgatgcta gataattcgc tagcaatcaa ttcttttgta ttcattaaga
     2101 gctccttttt ggacttttct actattttat cacaatttta aagaaagaag aaaaaatttt
     2161 tgaaatctcc tgtttttttg gtataatatg gttataaata tagttataaa tatgcacgca
     2221 agaggatttt atgagaaaaa gagatcgtca tcagttaata aaaaaaatga ttactgagga
     2281 gaaattaagt acacaaaaag aaattcaaga tcggttggag gcgcacaatg tttgtgtgac
     2341 gcagacaacc ttgtctcgtg atttgcgcga aatcggcttg accaaggtca agaaaaatga
     2401 tatggtgtat tatgtactag taaatgagac agaaaagatt gatttggtgg aatttttgtc
     2461 tcatcattta gaaggtgttg caagagcaga gtttaccttg gtgcttcata ccaaattggg
     2521 agaagcctct gttttggcaa atattgtaga tgtaaacaag gatgaatgga ttttaggaac
     2581 agttgctggt gccaatacct tattggttat ttgtcgagat cagcacgttg ccaaactcat
     2641 ggaagatcgt ttgctagatt tgatgaaaga taagtaaggt cttgggagtt gctctcaaga
     2701 cttatttttg aaaaggagag acagaaaatg gcgatagaaa agttatcacc cggcatgcaa
     2761 cagtatgtgg atattaaaaa gcaatatcca gatgcttttt tgctctttcg gatgggtgat
     2821 ttttatgaat tattttatga ggatgcggtc aatgctgcgc agattctgga aatttcctta
     2881 acgagtcgca acaagaatgc cgacaatccg atccctatgg cgggtgttcc ctatcattct
     2941 gcccaacagt atatcgatgt cttgattgag cagggttata aggtggctat cgcagagcag
     3001 atggaagatc ctaaacaagc agttggggtt gttaaacgag aggttgttca ggtcattacg
     3061 ccagggacag tggtcgatag cagtaagccg gacagtcaga ataatttttt ggtttccata
     3121 gaccgcgaag gcaatcaatt tggcctagct tatatggatt tggtgacggg tgacttttat
     3181 gtgacaggtc ttttggattt cacgctggtt tgtggggaaa tccgtaacct caaggctcga
     3241 gaagtggtgt tgggttatga cttgtctgag gaagaagaac aaatcctcag ccgccagatg
     3301 aatctggtac tctcttatga aaaagaaagc tttgaagacc ttcatttatt ggatttgcga
     3361 ttggcaacgg tggagcaaac ggcatctagt aagctgctcc agtatgttca tcggactcag
     3421 atgagggaat tgaaccacct caaacctgtt atccgctacg aaattaagga tttcttgcag
     3481 atggattatg cgaccaaggc tagtctggat ttggttgaga atgctcgctc aggtaagaaa
     3541 caaggcagtc ttttctggct tttggatgaa accaaaacgg ctatggggat gcgtctcttg
     3601 cgttcttgga ttcatcgccc cttgattgat aaggaacgaa tcgtccaacg tcaagaagta
     3661 gtgcaggtct ttctcgacca tttctttgag cgtagtgact tgacagacag tctcaagggt
     3721 gtttatgaca ttgagcgctt ggctagtcgt gtttcttttg gcaaaaccaa tccaaaggat
     3781 ctcttgcagt tggcgactac cttgtctagt gtgccacgga ttcgtgcgat tttagaaggg
     3841 atggagcaac ctactctagc ctatctcatc gcacaactgg atgcaatccc tgagttggag
     3901 agtttgatta gcgcagcgat tgctcctgaa gctcctcatg tgattacaga tgggggaatt
     3961 atccggactg gatttgatga gactttagac aagtatcgtt gcgttctcag agaagggact
     4021 agctggattg ctgagattga ggctaaggag cgagaaaact ctggtatcag cacgctcaag
     4081 attgactaca ataaaaagga tggctactat tttcatgtga ccaattcgca actaggaaat
     4141 gtgccagctc actttttccg caaggcgacg ctgaaaaact cagaacgctt tggaaccgaa
     4201 gaattagccc gtatcgaggg agatatgctt gaggcgcgtg agaagtcagc caacctcgaa
     4261 tacgaaatat ttatgcgcat tcgtgaagag gtcggcaagt acatccagcg tttacaagct
     4321 ctagcccaag gaattgcgac ggttgatgtc ttacagagtc tggcggttgt ggctgaaacc
     4381 cagcatttga ttcgacctga gtttggtgac gattcacaaa ttgatatccg gaaagggcgc
     4441 catgctgtcg ttgaaaaggt tatgggggct cagacctata ttccaaatac gattcagatg
     4501 gcagaagata ccagtattca actggttaca gggccaaaca tgagtgggaa gtctacctat
     4561 atgcgtcagt tagccatgac ggcggttatg gcccagctgg gttcctatgt tcctgctgaa
     4621 agcgcccatt taccgatttt tgatgcgatt tttacccgta tcggagcagc agatgacttg
     4681 gtttcgggtc agtcaacctt tatggtggag atgatggagg ccaataatgc catttcgcat
     4741 gcgaccaaga actctctcat tctctttgat gaattgggac gtggaactgc aacttatgac
     4801 gggatggctc ttgctcagtc catcatcgaa tatatccatg agcacatcgg agctaagacc
     4861 ctctttgcga cccactacca tgagttgact agtctggagt ctagtttaca acacttggtc
     4921 aatgtccacg tggcaacttt ggagcaggat gggcaggtca ccttccttca caagattgaa
     4981 ccgggaccag ctgataaatc ctacggtatc catgttgcca agattgctgg cttgccagca
     5041 gaccttttag caagggcgga taagattttg actcagctag agaatcaagg aacagagagt
     5101 cctcctccca tgagacaaac tagtgctgtc actgaacaga tttcactctt tgatagggca
     5161 gaagagcatc ctatcctagc agaattagct aaactggatg tgtataatat gacacctatg
     5221 caggttatga atgtcttagt agagttaaaa cagaaactat aaaaccaaga ctcactagtt
     5281 aatctagctg tatcaaggag acttctttga caattctcca cttttttgct agaataacat
     5341 cacacaaaca gaatgaaaag gagctgacgc attgtcgctc ccttttgtct attttttaag
     5401 gagaaagtat gctgattcag aaaataaaaa cctacaagtg gcaggccctg gcttcgctcc
     5461 tgatgacagg cttgatggtt gctagttcac ttctgcaacc gcgttatctg caggaagtct
     5521 taggcgccct ccttactggg aaatatgaag ctatttatag tatcggggct tggttgattg
     5581 gtgtggccgt agtcggtcta gttgctggtg gactcaatgt tgtcctcgca gcctatattg
     5641 cccaaggagt ttcatccgac cttcgggagg atgccttccg taaaattcaa accttttctt
     5701 atgctgatat tgaacaattt aatgcgggaa atctagtcgt tcgaatgaca aatgatatca
     5761 accagattca gaacgttgtc atgatgacct tccaaattct tttcagactt cccctcttgt
     5821 tcatcggttc gtttatccta gcggttcaaa ccttaccttc tctgtggtgg gtgattgttc
     5881 tcatggtagt cttgattttt ggtttgactg ctgtcatgat gggaatgatg gggcctcgtt
     5941 ttgccaagtt tcaaaccctt cttgagcgca tcaatgccat tgccaaggaa aatttacgtg
     6001 gcgttcgtgt ggtcaagtcc tttgtccaag aaaaagagca atttgctaag tttacagagg
     6061 tctcagacga gcttcttggt caaaaccttt acattggtta tgccttttca gtagtggaac
     6121 cctttatgat gttggttggt tacggggcgg tcttcctctc tatttggctg gtcgcgggaa
     6181 tggttcagtc ggatccgtct gttgttggtt ccatcgcttc ttttgttaat tacctaagcc
     6241 agattatctt taccattgtt atggttggat ttttgggaaa ttctgtcagc cgtgccatga
     6301 tttccatgcg tcgtattcga gaaattcttg acgcagagcc agctatgacc ttcaaggata
     6361 tcccagatga agagttggtt ggaagtctta gctttgaaaa tgtgaccttt acctatccaa
     6421 tggacaagga accgatgctg aaagatgtga gctttactat tgaacctggt caaatggttg
     6481 gtgtagttgg agcgactggt gcaggaaagt caaccttggc tcaattgatt ccacgtctct
     6541 ttgatccaca ggacggggcc attaaaatcg gtggcaagga tattcgagaa gtgagtgaag
     6601 gaaccctgcg taaaacagtt tccatcgttc tccaacgtgc cattcttttt agtggaacga
     6661 ttgcagataa cttgagacag gggaagggga atgctactct atttgaaatg gagcgcgcag
     6721 ccaatattgc ccaggctagt gaattcattc atcgtatgga gaaaaccttt gaaagtccag
     6781 ttgaagaacg gggaaccaat ttctctggtg gacaaaaaca aaggatgtcg attgcgcgtg
     6841 ggattgtcag caatccacgt attctgattt ttgatgattc gacctcagcc ttggatgcca
     6901 aatcagagcg cttggtgcaa gaagctttga ataaggactt gaaggggacg acaaccatta
     6961 ttattgctca aaaaattagc tcggttgtcc atgcagacaa gatcttggtt ctaaatcaag
     7021 gacgattgat tggtcaaggt acgcatgcag acttggttgc caacaatgcc gtttaccgtg
     7081 aaatctatga aacacagaaa tgaaagacaa actataagaa aagtcaatag ttttatctaa
     7141 actatttctt atttcaattt gatgatttgg cgatgatttt agagcacggc aaaaagccct
     7201 tgaaaaagtc cattttttca aaggtaatcc tgtgttaatt tcagaaatta catcactttt
     7261 tgttcgtcaa atggcagctc tttttttagg atataaaaca gggttcggat aagttttttt
     7321 gcaaggtgga tgatggctac attgtaatgt tttccttgtt ctaatttagt cttaagatag
     7381 gccttaaaag caggcgaaaa gcgagggcat gctttggcag cttgtatgag tacctaccgc
     7441 agatgagggg aactccgttt gaccattctt cctgctaaat caatctgatc tgactgataa
     7501 atagaagaat ccagtccagc gaaagcttgt aattgagcag gattatcaaa ggcatgaata
     7561 tttcgaatct cagctaaaat gaccgcccct aaacgatccc caatcccagt aaccgtcgtg
     7621 atgaccgagt tgaactcagc catcaagtca ttgacacatg tttccgcctt gtcaatgagc
     7681 ctcttgtaat gtttgatgtt ttcattacac gagataaaac gtctatgcgt tatcaaactc
     7741 attaccaatt aaaacaaaaa gctgtggtta gatcctttcg gaaattgtca agcgattgga
     7801 ggaaatgaac taatccacag cggcttattc caagtatacc acttgggctt tggcagtagc
     7861 taactgcgct aaatataata taaggaggag taaaatgaag acagttcaat ttttttggca
     7921 ttattttaag gtctacaagt tctcatttgt agttgtcatc ctgatgattg ttctggcgac
     7981 ttttgcccaa gccctctttc cagtcttttc tggacaagcg gtgacgcagc tagccaattt
     8041 agttcaagct tatcaaaatg gcaatccaga acttgtatgg caaagcctat caggaatcat
     8101 ggtcaatctt ggcctgctgg ttttggttct atttatctct agtgtaatat acatgtgtct
     8161 catgacgcgc gtgattgcag aatcgaccaa cgagatgcgc aaaggcctct ttggtaagct
     8221 tgctcagttg acggtttctt tctttgaccg tcgacaagat ggcgatatcc tgtctcattt
     8281 taccagtgat ttggataata tcctccaagc ctttaacgaa agcttgattc aggtcatgag
     8341 caatattgtt ttatacattg gtctgattct tgtcatgttt tcgagaaatg tgacgctggc
     8401 tctcatcacc attgccagca ccccattggc tttccttatg ctgattttca tcgtgaaaat
     8461 ggcacgtaaa tacaccaacc tccagcagaa agaggtaggg aagctcaacg cctatatgga
     8521 tgagagcatc tcaggccaaa aagccgtgat tgtgcaagga attcaagagg atatgatggc
     8581 aggatttctt gaacaaaatg agcgcgtgcg caaggcaacc tttaaaggaa gaatgttctc
     8641 aggaattctt ttccctgtca tgaatgggat gagcctgatt aatacagcca tcgtcatctt
     8701 tgctggttcg gctgtacttt tgaatgataa gtctattgaa acaagtacag ccctaggttt
     8761 gattgttatg tttgcacaat tttcacagca gtactaccag cctattatcc aagttgcagc
     8821 gagttgggga agccttcagt tggcctttac tggagctgaa cgaattcagg aaatgtttga
     8881 tgcagaggag gaaatccgac ctgaaaaggc tccaaccttc actaagttgc aagaaagtgt
     8941 tgaaatcagt catatcgatt tttcatactt gcctgataaa cctattttga aagatgtcag
     9001 catttctgcc cctaaaggcc agatgacagc agttgttggg cctacaggtt caggaaaaac
     9061 gactattatg aacctcatca atcgctttta tgatgttgat gctggtggta tttattttga
     9121 tggtaaagac attcgtggct atgacttaga tagtcttaga agcaaggtgg gaattgtatt
     9181 gcaagattcg gtcttgttta gcggaacgat tcgagacaat atccgatttg gtgtgccaga
     9241 tgctaatcag gaaatggttg aggtagcagc aaaagcaacc cacattcacg actatatcga
     9301 aagtttgcct gataagtacg atactcttat tgatgatgac cagagcatct tttcaacagg
     9361 gcagaagcaa ttgatttcaa tcgctcgaac cctgatgaca gatccagaag ttctcattct
     9421 cgatgaagca acttcaaacg tagatacggt gacggaaagc aagattcagc atgccatgga
     9481 ggtggttgta gcaggtagaa ctagtttcgt cattgcccac cgcttgaaaa ccattctcaa
     9541 tgcagatcag attattgtcc ttaaagatgg agaagtcatt gaacgtggta accaccatga
     9601 acttttgaag ctaggtggct tttattcaga actctatcac aatcaatttg ttttcgaata
     9661 agaaagaagt tgtcctatgt gggcagcttt ttcttgtcca taaaaaatgt ttatcacagc
     9721 cttaaaaaaa acatattaga cgaaagtcat tttgagtgat atgataggac tatcgttagt
     9781 attcgaaagg agaggcatca tggctagaac ggttgtagga gttgctgcaa atctatgtcc
     9841 cgtagacgca gaaggcaaaa tcattcattc atctgtatct tgtagattcg cagagatcat
     9901 tcgtcaagtc ggtggtctcc ctttagtcat tcctgttggt gatgagtcag ttgtacgtga
     9961 ttatgtggaa atgattgaca aactcatttt gacaggaggc caaaatgttc atcctcagtt
    10021 ttatggagag aaaaagaccg tcgaaagcga tgattacaat ctggtccgtg acgaatttga
    10081 attggcactc ttgaaggaag cgcttcgtca gaataaacca attatggcaa tctgtcgcgg
    10141 tgtccaactt gtcaatgttg cctttggtgg aaccctcaat caagaaatcg aaggtcactg
    10201 gcaaggacta cctttcggga catctcactc tattgagaca gtagaaggaa gcgtggtggc
    10261 taagctattt gggaaagaaa gtcaggtcaa ctcagtccat cgtcaaagca ttaaagattt
    10321 ggcacctaat ttccgtgtaa ctgctattga ttcaagagac caaactattg aagcgattga
    10381 gtctatcgac gagcaccgca ttatcggttt acagtggcat ccagagtttc tggttaatga
    10441 agaagatggc aatttagaat tatttgagta tttattgaat gaactgtaac gattggaaca
    10501 tctggtcgtt ctttctttta ttttttcaaa atttttagaa tgtggcattt tacgcaaacg
    10561 tttgaattct gataaaaatt ggagaaattc gacaaaaaaa cttgaaaaaa acgaaggtaa
    10621 gcgttatgat agaaaagaag aaatattgga ggaagaacat gtcacatatt aaatttgatt
    10681 attcaaaagt tttagacaaa tttgttgcac cacatgaagt ggaatacatg caatcacaag
    10741 taacagcagc agatgaattg atccgtaaag gaactggtgc tggtagcgac ttcttgggat
    10801 ggttggacct tcctgaaaaa tacgaccgcg aagaatttga ccgcatcttg aaagctgctg
    10861 agcaaatcaa gtcagacagc gatgttttgg ttgtgatcgg tattggtgga tcttaccttg
    10921 gtgcaaaagc agcaatcgac ttcttgaatc accattttgc taacttgcaa acaaaagaag
    10981 aacgcaaagc tccacaaatc ctttacgcag gaaactcaat ctcatctact taccttgctg
    11041 acttggtaga gtatgttgca gacaaagact tctcagtaaa cgtaatttct aaatcaggta
    11101 caacaactga accagcgatt gctttccgtg tctttaaaga actcttggtt aagaaatacg
    11161 gtcaagaaga agctaacaaa cgtatctatg caacaactga ccgccaaaag ggtgctgtta
    11221 aggttgaagc agacgctaac ggttggggaa catttgttgt tccagatgat atcggtggac
    11281 gcttctcagt attgacagcc gttggtttgc ttccaatcgc agcatcagga gctgacataa
    11341 aagctcttat ggaaggtgcg aatgcagctc gcaaagacta cacttcagac aaaatctctg
    11401 aaaacgaagc ttaccaatac gcagctgttc gtaacatcct ttatcgtaaa ggctatgcaa
    11461 ctgagatctt ggtaaactat gagccatcac ttcaatactt ctcagaatgg tggaaacaat
    11521 tggctggtga atcagaagga aaagaccaaa aaggtatcta cccaacttca gccaacttct
    11581 caactgactt gcactcactt ggtcaattta tccaagaagg aactcgtatc atgtttgaaa
    11641 cagttgtccg tgttgacaaa cctcgtaaaa acgtgattat tcctactttg gaagaagacc
    11701 ttgacggact tggttacctt caaggaaaag acgttgactt tgtaaacaaa aaagcaactg
    11761 acggtgttct tcttgcccac acagatggtg atgtaccaaa catgtatgtg actcttccag
    11821 agcaagacgc tttcactctt ggttacacta tctacttctt cgaattggca attgcccttt
    11881 caggttactt gaatgctatc aacccatttg accaaccagg tgttgaagct tataaacgta
    11941 acatgtttgc ccttcttgga aaaccaggat ttgaagaatt gagcaaagaa cttaacgcac
    12001 gtctataata gaagaaaaga gtggtttgcc cactcttttt actctcttta tccatagaaa
    12061 ttggactcag ccaagacttg tgatataata tagaaagcaa aaaggcagac gcctagataa
    12121 taggagaaac tatgtcaaaa gatatccgcg tacgttacgc accaagtcca acaggactac
    12181 tacacatcgg aaatgcccgt acagcattgt ttaactactt gtatgcgcgc catcatggtg
    12241 gaacatttct catccgtatc gaagatactg accgtaaacg ccatgtcgag gatggtgaac
    12301 gttcacaact tgaaaacctt cgctggttag gcatggattg ggatgaaagt ccagaatcac
    12361 atgagaatta tcgccagtct gagcgtttgg acttgtatca aaaatacatt gaccaactat
    12421 tagctgaagg aaaagcctat aaatcttacg ttacagaaga agagttggca gctgaacgtg
    12481 aacgtcaaga agctgctggt gaaacacctc gatatatcaa cgaatacctt ggtatgagtg
    12541 aagaggaaaa agcggcttat atcgcagaac gtgaagcagc tggtatcata ccaacagttc
    12601 gtttggctgt caatgagtca ggtatctaca agtggcatga tatggtcaaa ggtgatatcg
    12661 aatttgaagg tggcaatatc ggcggtgact gggttatcca aaagaaagac ggttacccaa
    12721 cttacaactt tgccgttgtt atcgatgacc acgatatgca aatctctcat gttatccgtg
    12781 gagatgacca tattgctaat acaccaaaac agcttatggt ctatgaagct cttggttggg
    12841 aagctccaga gtttggtcac atgaccttga ttatcaactc tgagactggt aagaagttat
    12901 ctaaacgtga tactaataca cttcaattta tcgaagatta ccgaaagaaa ggttaccttc
    12961 cagaagcagt ctttaacttt attgctcttc ttggttggaa cccaggtggt gaagatgaga
    13021 ttttctctcg tgaagaactc attaaacttt ttgatgaaaa ccgcctcagc aagtcaccag
    13081 cagcctttga tcagaaaaaa ctagactgga tgagcaatga ttatatcaag aatgcagacc
    13141 ttgaaaccat ctttgaaatg gcaaaaccat tcttagagga agcaggccgt ttgactgaca
    13201 aggctgaaaa attagttgag ctctataaac cacaaatgaa atcagtagat gagattatcc
    13261 cattgacaga tcttttcttc tcagatttcc cagaattgac agaagcagag cgcgaagtca
    13321 tgacgggtga aacagttcca acagttcttg aagcattcaa agcaaaactt gaagcgatga
    13381 cagatgataa atttgtgaca gaaaatatct tcccacaaat taaagcagtt caaaaagaaa
    13441 caggtattaa agggaaaaat cttttcatgc ctattcgtat cgcagtttca ggcgaaatgc
    13501 atgggccaga attaccagat acagttttct tgcttggacg tgaaaaatca attcagcata
    13561 tcgaaaacat gctaaaagaa atctctaaat aaagaggata caataatgga catctgggaa
    13621 aagatgtacg aagaagcaca gaaactatac aatccacatg aagtatcaga ctttgtttat
    13681 gccaatcatg ttgtagctgc agtagaagca gaagatggac aaatatttac aggattctgt
    13741 atggagggaa cctgtggtgt tttccatctc tgcgcagaac gggcggccct cttcaatatg
    13801 taccaatttt caggacaaac taaggttaag aaagtattag cctttcgaga caaaccacct
    13861 tatggtggaa gttcagccat gccttgcggt gcttgtagag aattcctttt agagcttaat
    13921 gctgaaaata aagatgcaga attcatgatg gactataata taagaaaaac agttaaagtc
    13981 gcagaactaa tcccttattg gtggggagaa gaacgtgctt ctaagtttaa tgagcgatag
    14041 aagagtcagt ataattctgg ctttttttac tataagttga aaaaat
//
LOCUS       DAVDWT010000044        13658 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVDWT010000044 DAVDWT010000000
VERSION     DAVDWT010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13658
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.044"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            209..556
                     /locus_tag="VUW50_001803"
     CDS             209..556
                     /locus_tag="VUW50_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET7983648.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     ADTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(836..1672)
                     /locus_tag="VUW50_001804"
     CDS             complement(836..1672)
                     /locus_tag="VUW50_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7983649.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKLSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(1685..2314)
                     /locus_tag="VUW50_001805"
     CDS             complement(1685..2314)
                     /locus_tag="VUW50_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET7983650.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(2324..2965)
                     /locus_tag="VUW50_001806"
     CDS             complement(2324..2965)
                     /locus_tag="VUW50_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET7983651.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(3111..4340)
                     /locus_tag="VUW50_001807"
     CDS             complement(3111..4340)
                     /locus_tag="VUW50_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7983652.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(4330..5496)
                     /locus_tag="VUW50_001808"
     CDS             complement(4330..5496)
                     /locus_tag="VUW50_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7983653.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFIAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            5640..6653
                     /locus_tag="VUW50_001809"
     CDS             5640..6653
                     /locus_tag="VUW50_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HET7983654.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VLTYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(6698..7117)
                     /locus_tag="VUW50_001810"
     CDS             complement(6698..7117)
                     /locus_tag="VUW50_001810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET7983655.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHFIDQERRAKIALQRAINRINVGNRL"
     gene            complement(7128..8534)
                     /gene="atpD"
                     /locus_tag="VUW50_001811"
     CDS             complement(7128..8534)
                     /gene="atpD"
                     /locus_tag="VUW50_001811"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET7983656.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(8620..9498)
                     /locus_tag="VUW50_001812"
     CDS             complement(8620..9498)
                     /locus_tag="VUW50_001812"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET7983657.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(9514..11019)
                     /locus_tag="VUW50_001813"
     CDS             complement(9514..11019)
                     /locus_tag="VUW50_001813"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET7983658.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(11034..11570)
                     /locus_tag="VUW50_001814"
     CDS             complement(11034..11570)
                     /locus_tag="VUW50_001814"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET7983659.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(11570..12064)
                     /gene="atpF"
                     /locus_tag="VUW50_001815"
     CDS             complement(11570..12064)
                     /gene="atpF"
                     /locus_tag="VUW50_001815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET7983660.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(12078..12794)
                     /gene="atpB"
                     /locus_tag="VUW50_001816"
     CDS             complement(12078..12794)
                     /gene="atpB"
                     /locus_tag="VUW50_001816"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET7983661.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(12829..13029)
                     /locus_tag="VUW50_001817"
     CDS             complement(12829..13029)
                     /locus_tag="VUW50_001817"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HET7983662.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(13104..>13658)
                     /locus_tag="VUW50_001818"
     CDS             complement(13104..>13658)
                     /locus_tag="VUW50_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET7983663.1"
                     /translation="STNFKPAGQSIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLV
                     LTDRKSRHQIIRLIPNKSAEVVNQALKLILKQHKILSITADNGTEFNRLFDVFSEEHI
                     YYAHPYASWERGTNENHNRLIRRWLPKGTKKMTPKEVAFIEKWINNYPKKCLDYKSPR
                     EDFWMANLNLKFSKMEIIFIKRFQ"
BASE COUNT         4183 a         3021 c         2439 g         4015 t
ORIGIN      
        1 ttgttttgtc gcttttcatt ataggtcata tgggactttt tttctacaca aaatagtctc
       61 cataatatct ataggggatt tacccactac aaatattata gagcctaaaa gatgagacca
      121 accaacctca tcttttttcg tatcaaatct aagcaatttt agaaacttta tggcatacta
      181 gacttatcaa tgaaagcgag gtaatctcat ggaacaattt ttagataaca tcaaagacct
      241 tgaagtcact acagttgtgc gtgcgcaaga agctcttgat aaaaaagaaa ctgcaacctt
      301 ctttatcggt cgcaaaactt gcccttactg ccgtaaattt gcagatacat tgtcaggtgt
      361 cgtagctgaa accaaagctc acatttactt catcaatagt gaagaaccaa gccaactcaa
      421 tgatttgcaa gcattccgct cacgctatgg aatcccaact gtaccaggtt ttattcatat
      481 tacagatgga caaatcaacg tccgttgcga ctcttcaatg tcagcacaag aaatcaaaga
      541 tttcgcagga ttgtaaaaaa tcagctaaca agtcttaaaa tcacaaaaac gcataatgta
      601 aggtgcaaaa accttgatat tatgcgtttt attgttccta aaacagttac atcagcattt
      661 cattacagaa cattttaatt ttggaagtca aatcaatttt taacaatggt ttagaaatca
      721 cagtgggcta ttccatattc aattatccag attcaatcgc tattatcgtt ttcctagatt
      781 acaaaaaaaa actacgtttt gctaatcaaa acgcagtctt agccactatt ttatcttact
      841 gtccttcttt tacttctttg gttgctacat cttctccaaa ccatttttgg ctgatttctt
      901 ggaacttgcc gtccttgtaa agactagaaa aagcttcatt tatcttctta accaaggttg
      961 tatcttcctt acgggctcca accgcaaaag cttctgtttc tagtccaact gtaaagacat
     1021 tataatcgtt taaaacacct tctgcttcta aataatagtt tgcatagaca cggtcaatca
     1081 atagaccatc aattcgatcg tttttcaaat caatcaaggc ttcattaaag gtttggtatt
     1141 gattcgcttc cttattagcg acaatattct tcaaaagttc tggatttgct tcaaagtccg
     1201 cataaccaga tgaaccagct tgagctccta atgtctttcc agtcatatcc tttgcagtcg
     1261 tgataccaga taatttcttc gtaaccaata cctgctcatt cttcatatat gagttactga
     1321 aagccacctt ttcacggcgt tcgtctgtag cggaatagcc attccaaatc agatcaatcg
     1381 ttccttttgt caattcagct tctttcaaat cccaatcaat cggttgccaa tttaccgtga
     1441 ttccgtattt ttcaaaaaca gctgtagcta aatcaatatc aaatcctgca taagaaccat
     1501 ctttctgagc aaatcccatt ggaacaaaag tactatcaaa tccaatagta atagacttgt
     1561 tagactggta ctttgaccaa ttatctccac tagtttcgct agaatttttc ccacaagcta
     1621 ctaagaacaa agcagtcatc agactgacta atacaagcat ccattttttc atccattttt
     1681 cctcctattt aggttctact ttcaataata catctgcgat attttcagca aactgcaaat
     1741 catgggtaac cacaatctgg gtcatcccaa gttccctatt ttgcaagatt agcttctcca
     1801 cttccaaacg taattctgga tccagggcag aagttggttc atcgtagcca atgatttctg
     1861 ggtcaatcat catagcacgc gccaaagcca ctcgctgctt ttgcccacca gatagtgaga
     1921 aaggataggc ctctgcgtgt cctcctagtc ctaactgttc caagagtcca ctcgccttct
     1981 tctcagcctc ttcctgcttc attcccatgg tcttcacagg cgataaagtc aaattttcca
     2041 gaactgatag atgaggaaat agttgaaaat cttggaagac aaatcccagt aggttgcgct
     2101 tctgcaattc atccagctct aaaggttgtc cattataaaa gatttgccct gaatcaatgg
     2161 tttcaagacc tgcaagcata cgtaaaagag ttgtcttacc tccaccagaa ggtccaacga
     2221 tagccaggat ttgcttttca ggaatactta gactgaaatt agacaggatt tgtttgtctc
     2281 caaagacttt attgatattt cgtaattcta acatggcagc ctcctatcta taataactat
     2341 acttcttctc aacttttttg gaaataattg tcacaatccc aatcaaaatc aaataaatgg
     2401 ctcctgccaa gaacatagga accagactag catcgcggtt agcagctgtt cgactagcca
     2461 agataaggtc tgaaattccg agagcataga ccaaagaagt atccttgacc aaactcataa
     2521 cttcattaaa gacactagga agaacgatct tggtcacttg gggcaagata atatagcgca
     2581 ctctgtcaaa agggctaaac ttcaagacct tggcagcctc atactgtcct cttggaatag
     2641 tgtcaattcc cccacggaaa atttctgcaa agtaagccgc ataattcaaa acaaaggcaa
     2701 taatagctgc aggaaggcgg tctaaacgaa tcccaatact tgggagcaca taatagataa
     2761 aaatcagttg caagagtaag ggggttcctc gcataaccca gatgtaaatg ttaatcagat
     2821 aatggagggg cttccaatgg acttgcaagg caaaggcaat caaaacgccc aagggaatcg
     2881 aaaatagcaa gaccagtgca aagacctgta cagtcgtgct cgcaccattc agtaaactcg
     2941 gtaatatctc aaataaataa gacatactgc acctcctaaa agtaattgtt cctattatag
     3001 catgaataaa caagattacc aagtattttt gtaaaaagat tctaccatat acaaataaaa
     3061 accgtagcta tcaaaagaga taaactacag ttctatatgt ttaaacattc ttacaatctc
     3121 tcaaacagtt cttgcatttg cacatcatct ggaaccagtt ttaagtaagt gtgagcatgg
     3181 acttttgctt cttcaaaatg tcccaattca cgcaagagat agatatagtg ttccagaaat
     3241 tctggattgt ccttcaaatc tcctgtcaac tcttgataat gctcataagc agtatccaaa
     3301 tcgtccattt cttgataaga acgagcaatc atccacttgg tcaaaagatt ttctggctcc
     3361 tcactctgca agtctagaat atcctcataa cgctcctgct ccagataaat agtggctaaa
     3421 cgaagcaaga tttcttctgt atcctcagcg tcttcttttg cagtaaggag ataattttct
     3481 gcaccactag catcatgcaa ttcataagaa aattgtgaag cagctagcaa gaggcgagtt
     3541 tcaaagggat ttttctctaa tccttgctta gcgatacgca gggcttcttg aacttgatgt
     3601 tccttatgta aagcctgact gtacccatac tcatagcctt caaagtcagg agaaatggta
     3661 tcaagctgct taaagtagag ggtggctttt tgatattctt cttgatcaaa ataaagactg
     3721 gccaactcaa aagctgttaa gtcatcgtat tctaactcca gggctttttc taaaaactca
     3781 gtagccgttt caaatttccc taactgagca taggcaaagc caattcgttg ataggtggaa
     3841 atgcccgttt gctcataaat cgagcgatta tctaactggg cataggcttg aatagccgct
     3901 tggtaatttt ccaactcact atccaactct gccaaaccca atatcaagag agaatcctct
     3961 gagtaggtca aggcctccaa taatttctca cgtgccacat ctgtcaaacc ttccagctgg
     4021 taaaggtctg ccttcagagc caaagacgag acataccagt cactgtcagc ttggatttcc
     4081 tcaagatagg taaaggcttc ttctatttga ccatcctcgc tagcaattgc agctagatta
     4141 agatgaacct ctggaaaatc ctctacaatt ttcaggtaaa tttccttggc ctgaggatag
     4201 aaaccaatcc cttcaagata agttgccaac tcatacagaa gatcacttga atcattttct
     4261 aaagctttgg cgaaataatg ctcagcctta gttaaatctt gctcctccaa agcctgtaac
     4321 atctgttgac tattgttcac tcttgacttc ctcaccctct aattcatata gaccactgac
     4381 taccttatac cattcgaaaa tggctgaaat gacaacctta gcagaggcat aaaccggaat
     4441 accaagtaaa actccccaga taccaaacat agatcctgaa gttaacaaaa caaagagaac
     4501 attaatagga tggatgttta attgacttcc caaaatcaat ggagagacaa aacggccttc
     4561 aatagtttgt tctacaataa agacaatcac tactttcaaa agcatgactg gaccagcaat
     4621 caaacccaat actagggcag gaagcatggc tagaaagcta ccaagataag ggaccagatt
     4681 taaaatacca gcagtaaccc ccagcgtaac cgcatagcgt agaccaataa tcttgaagaa
     4741 gatgataaac attactgcta caataatagc cactgtcact tgccctcgaa catagttgga
     4801 caactgttga ttcacatctg atagaacttg tccaacaggt tccttcaatt ttcttggaat
     4861 gaattgggtc aaatagttac gcaagccttt cccatcacgc aagagataaa agagcatgaa
     4921 aggaacgata atcaaggcaa caatcacttg agaagctccg ctaataaagg cactcaccca
     4981 gttgactgcc tgagatgaaa ccttacttgc caaaactgta gcctggctag aaaaattggt
     5041 caaaacttgc tctaattgag gtctgaaatc atctggcagg tgctgggcta ccaatccatt
     5101 aacaatccta tctatatctt ctaagtaaac aggaacgttt cttgcaaagg tcaaaacctg
     5161 acgttgcaga tttggaatgg cgactgccaa gccccaaatg ataaagagag cgatgataac
     5221 aaagacaata gtgatagcta taacacgatt aaccttatgc ttctccatcc aatcaacaat
     5281 aggattcaac aaataatata acaaaccaga caaaatgact ggcaacatca caactgctat
     5341 aaagtctaaa acaggtgaaa atagaaaact aatcttactt aaaataaaaa gattcaatcc
     5401 caataataag gttactaaaa ataccgtaat tgccttgtta tctaaaaacc acttgaaaaa
     5461 ccaagatagg ctaaaatgtt tctctttttg ctccataaat acttcctttc tattgttctc
     5521 tcattatacc atttttgaaa caaaataact cttttttaaa gtgcttacat ctaattgaat
     5581 gtctttaatt tgcttgtttt tgtgatataa tagtcttatc tttacacaga ggtatagtaa
     5641 tgaaagaaac tgtttatttt ggaacttata cacgtcgtac ttctcaaggg atttacaagg
     5701 cagactttga tacagaaact ggtcagcttt caaatctaga actttttgca gctgagccaa
     5761 gtccaaccta ccttgccttt gaccagcacc aacatttata cactgttggt agccaagacg
     5821 ataagggggg aattgcagcc tatcaaactg acgggactgt gttaaatcat gttgttgaag
     5881 aaggagctcc ccactgttat gttgctgtcg atgaaaagcg tgatttggtt tacgcagcca
     5941 actatcacaa gggacaagtc cttgtttata aacgccagga agatggtagt cttctactta
     6001 gtgatatgga tcaacacagt ggccaaggtc cacatgaaaa tcaagcttcc ccccatgttc
     6061 actatacaga tttaacacct gaccactatc tagtgacctg cgacttggga actgaccaag
     6121 tcctcaccta tgacctcgat caagaaggaa aattatctaa gctctatacc tatcacagca
     6181 agccaggagc aggctcacgc catatcattt tccataacca ctataaaatc gcttatctca
     6241 tttgtgaact caatagtact atcgaagttt taatctacga tggcgttggc gaatttgaac
     6301 gtatgcaggt tatttcaact ttaccagaag cttacgaagg ctttaatgga accgctgcta
     6361 ttcatctctc taaagacggt aaatacctct acgcttctaa ccgtggccat gattctatcg
     6421 cagtatatac catccttgcg gacggtagct tagagttatt agaaatcgtt ccaacgcatg
     6481 gtcagactcc acgtgatttt gatttgacac ccgaccaaaa atttctcatt gttgtccatc
     6541 aagactctga caatgcaact gtctttaaac gtaattgtga caatggtcgt ctagcagaac
     6601 tctccaacga cttccatgtt cccgaagcag tctgcatccg ttttgctcct taaaacacca
     6661 taaaaaatga acttggtatt ttcaagttca ttttttctta tagtctattt ccgacattaa
     6721 tacggttaat agcacgttgc aaagcaatct tagcacgacg ttcttggtca ataaaatgtt
     6781 tgtcttgtgc ttcttcaatt gcacgttctg cacgaagttt ggcacgttct gcacgactga
     6841 tatcgatatc acgagcacgt tctgcagagt cagcgacgat tgtgatcata tcattggcaa
     6901 tttcaataac gccaccgttt actgcaatcc agttcacgtg atctttatca tcgatacgtt
     6961 ttacctttac ttcatcaact gctaaaaccg caatcatatt ttcatgtcgt ggcaagatcc
     7021 ccatctcacc atccagagtt cgaaccgata catagctggc atggtgatca tagacgagac
     7081 catctggtgt cacgatctgg acagttaact gagccataga tcacctctta aaatcccatt
     7141 ttttcagctt ttgcaatcac atcttcgata gaacctacac cacggaaggc atcttctggc
     7201 aagtggtcgt atttaccatc aaggatttcc ttaaagccac gtacagtttc agcaactgga
     7261 acataagaac ctggctgacc agtaaattgt tccgcaacgt tgaagttttg tgacaagaag
     7321 aactggatac gacgggcgcg agcaaccaag gtcttttctt catcagaaag ctcatccata
     7381 ccaaggatag caatgatatc ttgcaattca tggtaacgtt gaaggacacg ttttacttca
     7441 gcagcaactg catagtgctc ttctccaacg atttcaggtg ccaaggcacg tgagcttgaa
     7501 gcaagtgggt caacggctgg gtagataccc aattgtacca acttacgttc caagtttgtt
     7561 gttgaatcca agtgagcgaa ggctgttgct ggcgctgggt cagtatagtc atccgctggc
     7621 acatagatag cctggataga ggttacagaa cccttcttgg ttgatgtgat acgttcttgc
     7681 aattgaccca tttccgtagc aagtgttggt tggtaaccaa cggctgatgg catacgaccc
     7741 aaaagggcag atacttctga accagcctga gtgaaacgga agatattatc gataaagaga
     7801 agcacgtctt ggccttccac atcacggaag tattcagcga ttgtcaaacc agtaagggca
     7861 acacgcatac gtgctcctgg tggctcattc atctgaccaa agaccatggc tgttttctcg
     7921 ataacgcctg attctttcat ttcccagtaa aggtcattcc cctcacgagt acgttcccca
     7981 acaccagtaa atactgaaat accaccgtgc tcttgggcaa tgttgtgaat caattcttgg
     8041 attaagacgg ttttaccaac tccggcacca ccgaaaagtc caactttacc acctttaagg
     8101 taaggggcaa gaaggtcaat aaccttgatc cctgtttcaa ggatttcaga agaggtagac
     8161 aactcatcaa aagttggagc ttttttatga attggctgac gctctgcgtc ttctgtaaaa
     8221 ggagcttcca aatcaatggt atctcccaaa acgttgaaga cacgtcccaa agtttcttta
     8281 cctactggta cagagattgg acgacctgtg tccaatactt ccattccacg agtcaaccca
     8341 tctgttgatt ccatggcgat agtacgaacc ataccatctc ctaactccaa ggctacttca
     8401 aggacgattt ttgtttttct ttcgtcattt ttgtagacga caagtgcatt gttaatctca
     8461 ggaagttttt cccctgctgc aaacaaaacg tctacaacgg gaccgataac ctgagcaatt
     8521 ttacctgaac tcatctcctt ctcctattct atataagata ctgtcggttc ctagctcaac
     8581 taggtcctaa gttcattttc atacgagctg gactagagcc tattctaagg cactagcacc
     8641 tgctacgatt tctgtaattt cttgtgtaat cgccgcctgt ctggcacggt tatactgaat
     8701 tgtcaaatca ttgatgactt tcttagcatt atctgtcgct gtttgcatgg ctgtcatacc
     8761 cgcagcattc tcagctgtct tggcatcgat aatggcaccg taaatcatac tttctgcaaa
     8821 ctgaggcaac aactgctcca gaatttcttc tcggctggtt tccaattcaa aagtcaagct
     8881 gtactcttca tccgcttcat ttggatccaa gtcaacaatc ggaagcattt gttccacacg
     8941 catttgactg gttagcgtat tgacatggtg gttgtagcaa acataaagct catcaaagag
     9001 ttcattttgg tacatttcaa cagtttttga aataatctta cgaacttgat caaagctagg
     9061 ttggtctgcc aagccacgta attcataaag tggttgaata ccgcgagcct taaagaaatc
     9121 agctcccatc ccaccgatac agatcatttc aaaaccttta ccgtctgggt ggtattcttc
     9181 tttcaactcc ataacagctt tcaaaataga ggaattataa cctccaacca aaccgcggtc
     9241 tgaagtgata acgatatagc ctgtcttctt cacagaacgg ctaatcaaca tcggattagt
     9301 tgaagcacca gctccattac catgaaggat atctgtcaaa agtttacgca ctttctgagc
     9361 gtaaacttgg aagttgcgag cagcttcttc agagcgacct aacttagcag ccgataccat
     9421 ttgcatggca ttagtgattt gactcgtatt ttttgttgag gcgatttttg ttttaatatc
     9481 atttagagac actgccatct gacacctcta ttcttattgg aagctagatt gattgagaaa
     9541 ctctgtaatc gcagcatcca agactgcttc ttctggcaag tcttttgtat cacgaatggt
     9601 ttccaaaatc tctggatgtt gagcatcaaa gaaggcatgg aactcttcct cgaaacgaac
     9661 aatatcatct actggaacag tatccaagaa accatgtgtc aaagcataaa gaatggttac
     9721 ttgtttctca acaggtaatg gtttgtgaac aggttgtttc aagacctcaa cggtacgacg
     9781 tccacggttc aacttagcct gtgttgctgc gtccaagtca gaaccaaact tagtaaaggc
     9841 ttccaactca cggtatgaag caaggtcgat acgaagtgta ccagcaacct tcttcatggc
     9901 tttgatttgt gcagaaccac ctacacgaga tacagatgaa cccgcatcga tggctggacg
     9961 aatacctgca ttgaagaggc catcgccaag gaagatttgt ccatcagtga tagaaatcac
    10021 gttggttgcg atataggctg agatatctcc tgcttgtgtc tcgataaatg gtagggctgt
    10081 aattgatcca ccaccaagtt catcagaaac tttagctgag cgctcaagca aacggctgtg
    10141 gagatagaaa acatcccctg ggaaggcttc acgacctgga ggacgacgaa gcaagagcga
    10201 tagttcacga taagctaccg cttgttttga tagatcatcg tatacaatca aaacatgctt
    10261 accttgatac ataaattctt ccgccatagc aaccccagta taaggagcta ggaagagcaa
    10321 tggagatggt tgtgaagcag aggctgtcac aacgattgtg tagtccaagg caccgtactg
    10381 acgaagtgtt tctacttgcg tacgaactgt tgattctttt tgtccaatcg cgacgtagat
    10441 acagatcata tcttgatctt tttggttcaa gattgtatca atcgcaatgg ttgttttccc
    10501 tgtctgacgg tcaccgataa tcaactcacg ttgaccacga ccaatcggta caagggcgtc
    10561 aatagctttc aaaccagttt gcaatggttc tgaaacagac ttacgttgca taacaccagg
    10621 agctggtgct tctactggac gagttttatc agtgtggatt tctccaagac cgtcaactgg
    10681 acgaccaagc ggatccacaa cacgaccaat cagactttca cctacaggga cttccatgat
    10741 tttccctgta cggcggattg tatcgccttc acggatatct gtaaagtcac ctaggatgat
    10801 aataccaacg tctgttgact ccaagttttg agccatacca taagagccgt tttcaaaatt
    10861 caacaactct ccactcatga cattttcaag gccatgagca cgcgcgatac cgtccccgat
    10921 ataggttaca acacctgttt cagtcacatc aaaattgggt ttgaaatttt caatttgttg
    10981 cttaattaaa gcgctgattt cttgtgcgtt aattgccaaa agaacaccac tttctatttc
    11041 aaattttctt taacaacttt aagttgttgt ttaatactca catcaattgt cttgtgattg
    11101 gcaaaaatga caaaaccacc aatgagactt tcatcgattt gttcttttac actccttact
    11161 ttcagagaca tttttttctc aatcaaaggg agcaaacgag tcttctgttc atcagttaga
    11221 ggatgagcag acgtaatcgt cacttcaaat cgatttgttt ctttttcaag tcggttcaag
    11281 caatctacaa gcacatcata aaaaagattt gctctgtgat tgtaggccag aacctggata
    11341 aagttttgta ataaaggcga cacagaatct tggaaaaaag caattgtttt ttccttatcc
    11401 gactcgtcta ctgccacttg ttttaaaaaa gaaggcagac ctgttttttc aacaacttgc
    11461 ttgatttgag tcaagtctga aaagatacgg tcttcttctc ctttttcaag taccaattgg
    11521 acaaaaggca tgctgtattt ttcaattacc tttactgttt tcttgtccat taagcttctc
    11581 ctagctgatc gatatactga tcaatgagtg ctttatgggc atgactgtca aggttttgtg
    11641 agatgatttt accagctaag ctgatggtca aatctgcgac ctcaccctta acactctgta
    11701 aagcttctac tttattttga gcaatttctt gattggcttt ttcttttaag tgtcctgctt
    11761 ctagtttagc atctgctaag atattagcct tactttgctc agctgtttcc tttgcatttt
    11821 caatgattgt cttagcttct ttacggctac cagccaattc atcttcgcgt ttttgagcca
    11881 atacttctgc tttttgacgg gcttcttcag ctctgtcaat atctgaagca attttttcag
    11941 ctctttcttc gaaaatgcct gtaatattag accatgcaaa ttttttaatc aagactagca
    12001 aaagaataaa agagccagtg attaaaataa aattaccaat taattcacct actgttacgt
    12061 gcatcagtta ctcctttcta ttcctcttca atattaatct tattccctaa atacacagat
    12121 gtcaaaagag taaaaacata agcttggatg caggaaataa agacagaaaa tgcagtccaa
    12181 gccaaattag ctccaaaggc tactggatac caataaatag cttggtggga aagaagaagt
    12241 aacaaactcg tcatgacctc tcctgcaaag atattcccaa aaatccgcaa agccaaagat
    12301 aagaagtttg taaattcttc caagatattc atcggtatga caaaaacagg actcataaaa
    12361 gattttatac tttttttaaa tccacgacga cgaacgcttt ctatatgtgt caacaaaatg
    12421 acaagaaaag ataaggttaa gtcatactgt aaattagcgg ttggcgaact ccaccagtta
    12481 gttccatcga tcgtttgaag ctttgtcatt aagccaaggt tattggcaat caccatgaaa
    12541 aggaataaac aaaggaaaaa gagtgagtaa tctttcatgt agcgcgaacc aatgttaggt
    12601 tctgtaaatc caataacaaa gtcatagaca tactcaagta cattttgctt tcctttgggt
    12661 ttcaaggtca tattgcggct tgcccaataa ataaagacaa aaataactcc cacaatcaac
    12721 aaagtcatgg ctaacatagt cagattgaag ataacaggac caatagagat gattggatta
    12781 atactttctt ccatctaaaa tcctcccttt tcttctcgtt ccatgtattt atttgataat
    12841 aaatgagaag acaagagtta caaagaaagt tccttcaata aaggcaacac ctaaaaacat
    12901 caaactacga aactcagaaa gcatatctgg ttggcgtgct actgatttaa acagtccatt
    12961 catcaataaa ccttcaccga cagatacgcc catacaggca atacataagc ctaaaaatgt
    13021 taaattcata acaaattctc ctttaaaata aaatttactt acttagtgta gtcctaaaat
    13081 ttaaatttgt caacttttaa ccgctattgg aagcgtttaa taaaaattat ttccattttg
    13141 ctaaatttca agttcaagtt agccatccag aagtcttctc tgggtgactt gtagtccaag
    13201 cattttttag gatagttgtt aatccacttt tcgatgaatg cgacttcttt gggagtcatt
    13261 ttcttggttc ccttaggtaa ccatctacga atgagcctgt tgtgattctc attagttccc
    13321 ctttcccaag aggcataggg gtgcgcataa tagatgtgct cctcagaaaa tacatcaaac
    13381 aagcgattga attccgttcc attatctgcc gtgatggaaa gaatcttgtg ttgttttaag
    13441 atgagtttta gagcctgatt gaccacctca gcacttttat ttggaatcaa tcggatgatc
    13501 tgatgtctac tctttcgatc cgtcaagaca agcaagcagt agtttttcgc tctcgtaagt
    13561 agaaccgtat caatctcata atgcccattc tccaagcgaa gattgatagc ttcagaccgc
    13621 tgttcgatgg attgaccagc aggtttaaag ttggtgct
//
LOCUS       DAVDWT010000045        13338 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVDWT010000045 DAVDWT010000000
VERSION     DAVDWT010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13338
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.045"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(82..615)
                     /gene="queF"
                     /locus_tag="VUW50_001819"
     CDS             complement(82..615)
                     /gene="queF"
                     /locus_tag="VUW50_001819"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HET7983664.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQRHHLE
                     IQIAREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKPLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            complement(921..1235)
                     /gene="trxA"
                     /locus_tag="VUW50_001820"
     CDS             complement(921..1235)
                     /gene="trxA"
                     /locus_tag="VUW50_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET7983665.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            complement(1252..1473)
                     /locus_tag="VUW50_001821"
     CDS             complement(1252..1473)
                     /locus_tag="VUW50_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET7983666.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLNYHGTYGDIFFYLMKQDLSQYN"
     gene            complement(1573..1953)
                     /locus_tag="VUW50_001822"
     CDS             complement(1573..1953)
                     /locus_tag="VUW50_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7983667.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            complement(2097..3050)
                     /locus_tag="VUW50_001823"
     CDS             complement(2097..3050)
                     /locus_tag="VUW50_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET7983668.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(3118..4327)
                     /gene="sstT"
                     /locus_tag="VUW50_001824"
                     /pseudo
     CDS             complement(3118..4327)
                     /gene="sstT"
                     /locus_tag="VUW50_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            4466..5026
                     /locus_tag="VUW50_001825"
     CDS             4466..5026
                     /locus_tag="VUW50_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HET7983669.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            5083..5991
                     /locus_tag="VUW50_001826"
     CDS             5083..5991
                     /locus_tag="VUW50_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET7983670.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEYRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            5943..6533
                     /locus_tag="VUW50_001827"
     CDS             5943..6533
                     /locus_tag="VUW50_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HET7983671.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            6536..7642
                     /gene="yqeH"
                     /locus_tag="VUW50_001828"
     CDS             6536..7642
                     /gene="yqeH"
                     /locus_tag="VUW50_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HET7983672.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            7857..8168
                     /gene="yhbY"
                     /locus_tag="VUW50_001829"
     CDS             7857..8168
                     /gene="yhbY"
                     /locus_tag="VUW50_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HET7983673.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            8220..8849
                     /locus_tag="VUW50_001830"
     CDS             8220..8849
                     /locus_tag="VUW50_001830"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET7983674.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGWKPNFLLPQPVLDYIEKEGLY"
     gene            8849..9442
                     /gene="yqeK"
                     /locus_tag="VUW50_001831"
     CDS             8849..9442
                     /gene="yqeK"
                     /locus_tag="VUW50_001831"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HET7983675.1"
                     /translation="MSYQDYINCSREALLKKMAEFLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVVYVADYIEHNRSFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            9443..9943
                     /locus_tag="VUW50_001832"
     CDS             9443..9943
                     /locus_tag="VUW50_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET7983676.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKSDEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            9956..10309
                     /gene="rsfS"
                     /locus_tag="VUW50_001833"
     CDS             9956..10309
                     /gene="rsfS"
                     /locus_tag="VUW50_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET7983677.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            10629..11369
                     /locus_tag="VUW50_001834"
     CDS             10629..11369
                     /locus_tag="VUW50_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7983678.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            11379..12476
                     /locus_tag="VUW50_001835"
     CDS             11379..12476
                     /locus_tag="VUW50_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET7983679.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRDIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQVRESNLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            12714..12968
                     /locus_tag="VUW50_001836"
     CDS             12714..12968
                     /locus_tag="VUW50_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HET7983680.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            12972..13226
                     /locus_tag="VUW50_001837"
     CDS             12972..13226
                     /locus_tag="VUW50_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HET7983681.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
BASE COUNT         3983 a         2590 c         3035 g         3730 t
ORIGIN      
        1 ttatctataa cctcaaacac tgctttgagc aacctgcggc taacttccta gtttgctctt
       61 tgattttcat tgagtatgag tttagcggtt gtcaattttc tctggataaa ggtcgtgttg
      121 gaagaggcgt tgttctgcca agccctcata cttagttcct tgcttaccgt agttatagta
      181 ggggtcgatt gaaatgccac cgcgcggagt gaattttccc cagacttcta aatagcgagg
      241 gtctagcaag ttgaccaagt ctttcccgat ggtgttgata cagttttcgt ggaaatctcc
      301 gtggtttcgg tagctaaata gatatagttt gaggggtttt gactcgacac agagcttgtc
      361 aggaatgtag gaaatatgaa tcgtcgcaaa gtctggctga gcagtgattt gtcccagcag
      421 agacatatcg aggatatggt gacgaatgcc ctgttcctta gcgatttctc tagcaatttg
      481 aatttcgagg tgatgacgtt ggccgtaggc aaaggtgaca gcttcgactg tttcatagtg
      541 ttgcatggcc cagaaaaggc aggttgttga atcttgacca ccactaaaga cgaccaaggc
      601 tgattgacgt ttcacagtac tccttccaaa atgggaaatg ttcagagcac gcaaaaagct
      661 cccattaggg agctaaaaaa taccaaatcg aggttttttt agcgatggca tatcccaaac
      721 atcgtaatat tctacttata tagtaaaatg aaataagaac aggacaaatc gatcaggaca
      781 gtcaaatcga tttctaacaa tgttttagaa gtagaggcgt actattctag tttcaatata
      841 ctatagtcta gcatattttt tgaaaaatag caaagggcaa gaaaaaagag accaaagaaa
      901 gtacttggtc tctcgtttga ttagctcaat tcagcaatga tggccttgat ttgttctgct
      961 gtgtgaacac ctgcaacttg tttgacaact tggccgtctt ttttgaagag aagagttgga
     1021 atagacatga ttccaaaagc acgagctgtg tttggatttt catcaacgtc cattttaacg
     1081 attttcaaga catcttctga aagttcttca gacaatttgt ccaagattgg accttgcata
     1141 cgacatggac cacaccaagt tgcccagaag tctactaaga ccaaaccgtc ttttgtttct
     1201 tgttcgaatg ttgcatctgt aattgctttt gccattgtat ttctcctttt tttagttata
     1261 ttggcttaaa tcttgtttca tgagatagaa gaagatatct ccataagtcc catggtagtt
     1321 caaattatga cccttgtaag ttaatttttg gacagggtag tagtctgcga cgccgataag
     1381 gcaagcttgt tgcgaacgtt caaagtcttc ataagactcg aaagtcacag ttctttcgtt
     1441 cttgctggca tctatatagg taatttcaat catatttaaa actcctttgt ttaatgctaa
     1501 ctttatttta ctccttataa aagagaatgt caagaaaaat gattgcgcac gcaacttttt
     1561 ttaaaaatca tcttaaatca agaaatccaa acctgcttcc aagctttctt cgacagtttt
     1621 ttgtagcgag gccagtgtct tttgcccatc atttgtcagg cagataaaac tagagcgtct
     1681 atcttgatgg caacacatgc gactgagtag accgcaattt ttagcttcca agcgagccac
     1741 catcctagaa actgcgctcg ggctcagatg aagtttatct ggcaggtcaa tctggcgtag
     1801 agatttttct tcagccaagt ccagatagta gagcaggtag aactctttca aggtcagact
     1861 ttgctcgctc tgttgggcaa tggtctcttc caagagactt tcaatttctt tctgacgccg
     1921 attgaagtca aaccattttt ccaaataggt catagtgtct cctttctttt tagagtcata
     1981 atatagaaga aagtccatta acgggcagtc tctgcgtcac aagatgattg cgcatgcaat
     2041 aattatacta cttttcaaga atgctggcaa gctctgtttt ttagtggttt tcttttttac
     2101 tgtctatatt tttggtaaaa atcaactttt acttggatga aggttttggc ttcacgtagg
     2161 agttgaagaa gggtggcgcg ggtttcaaat tcttctcttg tcttgggcag actgcggttc
     2221 cggaagactt ccagataacg ttcaatttca tctagcaaat cagaagcagg attggtctgg
     2281 ctcagttgac ctgcaatttt tgaaaagagt tgcgctaaga tcaggctttc actggcggca
     2341 aggtgacaag tgttaatctg ttgggccatg tttctcagta tacgactttg tcgttgtctc
     2401 atctcaaagt agtggatatg gtagtcggtc tggtgaaaga ggtggtcaga gtgatccaaa
     2461 tagaccagtc tgagggcttc tttcaaaagc gtgtctaatt ctgctaccag ctgtgctcgg
     2521 ttgcgtccgt ctcctctgga taaatagtat ttgaagcgct ggaggatatc ttttaacttt
     2581 tcttccacca gcgtgtggta gtgctggatt tcctcttctc gtgaaggcat atagagatta
     2641 acaagcaagg caaatcctgt accaatagca aagagaagga attcattgac tagaaggtct
     2701 ggagaggttg actcttgaac caagagatgg ctaaccaaaa cagtgcttgg tgtgatgcca
     2761 atttcccagc ccatcttgta ggctaaagga acgtagaagg ccagatagag gccgagactc
     2821 cagatatgaa atccgctcaa gtgaaaagct agaacaccga tagccagagc tagaagcata
     2881 gaaaaaagac gattgcgagc cagttttaaa gtacttctac gcgtatcaga taggctcaag
     2941 agagcgataa ttccagccga aactgctgac gaaagattga gaaaataagc aagcaggcag
     3001 gcaagacagg tagctaagat gagcttggtc gtacgttggc taatagacat aagaatttcc
     3061 taataagtta gaataaaagc gtaaaagaca agacatgagc aggcttgcct tgatgagtta
     3121 ttttttacgg gttgctgcgt attcggcaac ggcggtaaag aggacatctg tagaagagtt
     3181 aagggctgtt tcacatgagt cttggatgac accaatcaca aaaccaaccc caacaacttg
     3241 tatggcaata tcgttagaaa taccgaaaag gctacaagca actgggataa gaaggaggga
     3301 acctccggca atacctgaag caccacaggc tgagatagct gctaccacac tgaggacaaa
     3361 ggctgtggca aagtcaacag gaattccaag agtgttaatt gtaacaaggg tcaaaaggtt
     3421 aatggtaatc gctactccag ccatattgat agtagaaccg agtgggatag aaacagaata
     3481 ggtatctggg ttgagtccaa ggtcatggca gagtttcatg ttgacaggaa tgttagtcgc
     3541 agaactacga gtgaaaaagg ctgtcacacc gctgacacgg aggcagttcc aaactagagg
     3601 gtaaggattg cgtctcataa agaagaaggc aatcaaaggg ttgaccacag gggcaacaaa
     3661 aagcatagtc gttactaatg gaaccaataa aataccgtag ttggcaaggc ttccgactcc
     3721 cttgtcagaa atggttttaa aaacaagacc aaggattcca aatggagcca gattgatgat
     3781 ccattcgaca attttagaag tcacgtcagc gatagttttt agcaattctt tactattttt
     3841 actggcttct ctcatagcga ttccaaaaat gactgcccaa gataagattc caatatagtt
     3901 agcagtaaac agggcgttga ctgggctgtc aaccagtttg agcaagaggt tgctgagaac
     3961 ctgcccaacc ccatctggtg gtgcaatttc agtattggca ctatttaggg taatttcaat
     4021 agggacgatg aaacttgcta gtacagctac aagagcagcg gcgaaagtcc ctatcatagg
     4081 atatacaaga aaacaacagt tttcatattg ctatcttgtc ccttttgatg ttgggaaagg
     4141 gcattggcaa cgagggcaaa gactaggata ggagcaacag cttttagacc tccaacgaat
     4201 aaatccccga gtagcccaat ccctgagaga ttaggaaggg tcagtcctag gattccccat
     4261 aaagcatacc aatcaagata cgcttgacaa ggcttgcctt attccaagca tgaatgattc
     4321 ttttcataat aatctccttt ttgtgtagtg attatgatta tagtataaat gatagacaaa
     4381 atcaagaatt ttctgtctat tttttgaata tttatggaga atgagactga tgaaaatatg
     4441 gtataatgaa ataaaggagt tttatatgca aaaatttatt caggcttata ttgaaaagct
     4501 agatgtgaca accattatcg agaatattct aaccaaggtc atttctcttt tactgctttt
     4561 aattgtattt tatattgcta aaaaaatgct tcataccatg gtgcagagaa ttgtcaaacc
     4621 ttctctaaaa atgtctcgtc atgatgttgg acgccaaaaa accatctcac gtttactaga
     4681 aaatgtgttt aattatacgc tatatttctt tttactctac tgcattttgt cgattttagg
     4741 tttgccagtt tctagtttgc tggctggagc tggtattgct ggggtagcga ttggtatggg
     4801 agcccaaggc tttctgtctg atgtcatcaa tggctttttc atcctctttg aacgtcaact
     4861 ggatgtggga gatgaggtcg ttctgacaaa tggaccgatt actgtatcgg gtaaggttgt
     4921 cagtgtggga attcgtacga cacagcttcg tagcgaggag caagcccttc actttgtccc
     4981 taaccgaaat atcacagttg ttagcaattt ctcacgcaca gactagacct gttattttaa
     5041 gtaatttgtg gtacaataga gggagtttaa taaggagaaa agatggtttt agaaaagcag
     5101 ttgggcaatg gttgtacctg gatagaccta gacctaggaa agttgaataa actagaagac
     5161 ctttctgaaa tttacggttt ggacaaggaa accattgaat acgcactgga tagaaacgag
     5221 cgcgcccaca tggactacca ccgtgaaagt gggacggtta cctttatcta taatgtccta
     5281 gacgtaaaaa aggacaaggc ctactatgag acttttccca tgacctttat tgtcgagtat
     5341 cgtcgcctga ttaccattag taataccaag aacgcctatg tcattgaaca gatgactcgt
     5401 tatctggaga gccatgacac gctttcgatt tataagtttc tctttgccag tctggaaatc
     5461 atcagcaatg cctactatcc tgtcattgag cagatggaca agagtaggga tgaggtcaat
     5521 gacctcttgc gccagcgaac taccaagaaa aacctctttg ccctgtctga tttggagact
     5581 ggtatggttt atctgacggc agctgccaaa caaaatcgga ttttgttaga gcatattcaa
     5641 ggtcatgcct tgtatcgtag ttttgatgag attgagagag aacagtttga tgatgccatg
     5701 attgaggctc atcagctggt atccatgaca gacctaatct ctcagatttt acagcagctt
     5761 tcagcctctt acaacaatat tctaaacaat aatctgaatg acaatttgac aaccttgact
     5821 atcatttcag tcttgctagc tgttttggca gtcgtgacag gctttttcgg aatgaatgtt
     5881 cccttacctt taacagatga gccccatgct tggctctata tcagtttggc tagtgcaggt
     5941 ttgtggattg ttttatcctt gttactaagg aaaattgcga aaaaaagtta agaaaaggag
     6001 ccagaatggc gattgaaaat tatataccag attttgctgt ggaagcagtc tatgatctga
     6061 cagtcccaag cctgcaggcg cagggaatca aggctgtttt ggtcgatttg gataataccc
     6121 tcattgcttg gaacaaccct gatggaacgc cagagatgaa gcaatggcta catgaccttc
     6181 gggacgcggg tattggcatt atcgtagtgt caaataacac caaaaaacgc gttcaacgag
     6241 cagttgagaa atttgggatt gattacgttt actgggcctt gaagcccttc acatttggta
     6301 ttgaccgtgc tatgaaggaa ttccactatg acaaaaagga agtggtcatg gttggcgacc
     6361 agctcatgac agatatacga gcagcccacc gtgcagggat tcggtcaatt ttagtcaaac
     6421 ccttggtcca acatgactca atcaaaacgc agattaaccg aactcgtgag cgtcgtgtta
     6481 tgagaaaaat cactgaaaag tacggaccga ttacatataa aaaaggaatt taactatgga
     6541 agaaattctc tgtattggtt gtggagcaac cattcagacg acagataaag ctggtcttgg
     6601 ttttaccccc cagtcggcac ttgaaaaagg tttggagact ggcgaagtct attgccaacg
     6661 ctgtttccgt ctccgccact acaatgaaat cacagatgtc cagttgacga acgatgattt
     6721 cctcaagctc ttgcacgagg tgggagacag tgatgcctta gtggtcaatg ttattgatat
     6781 ctttgatttt aatggatctg tcatcccagg tttaccacgt ttcgtctcgg gcaatgatgt
     6841 cctcttggta ggaaataaaa aagatatcct tcctaagtca gttaagtctg gtaagattag
     6901 ccagtggctc atgaaacgtg cccatgaaga aggtcttcgt ccagtcgatg tggtcctaac
     6961 ttcagcacaa aataaacatg ccattaagga agtcattgac aagattgaac actaccgtaa
     7021 gggccgcgat gtctatgtgg tcggtgtgac caacgttgga aaatcaactc taatcaatgc
     7081 tattatccaa gaaatcacgg gtgatcagaa tgtcatcact acttcacgct tcccagggac
     7141 aaccttggac aaaatagaga ttccgcttga cgacggatct tatatttacg atacgccggg
     7201 aattatccac cgccaccaga tggctcacta cttgacggcc aaaaacctca agtatgtcag
     7261 tcctaaaaag gaaatcaagc ctaagaccta tcagcttaat cctgagcaaa ccctattttt
     7321 aggtggtttg ggacgctttg actttatagc aggagaaaag caaggattta ctgctttctt
     7381 tgataatgaa ctcaaactcc atcgtagcaa gcttgaagga gctagtgctt tctacgataa
     7441 gcacctggga actcttctga caccaccaaa tagcaaggaa aaagaagatt tcccaaggct
     7501 agtccagcat gtctttacca ttaaagataa gacagaccta gtcatctcag gcctaggctg
     7561 gattcgtgta acaggcatag caaaagtcgc cgtctgggca ccagaaggcg tcgccgtcgt
     7621 cacacgaaaa gcaattattt aagcacagaa aggaaagggt tgtctgaatt tgggcgagca
     7681 aggcgagccc catagagaat acttttcgct gtggtgtaag ttggtacaag tgattgtacc
     7741 aactgcggaa aatttgagac cttaggctca aattttagtc atgaaagtcc gaaggacttt
     7801 gctgacgtcc gtcaccactt cagaaaagta taaaaagaaa ctcttttaaa gaaattatgt
     7861 cattaacatc aaaacaacgt gccttcctca acagccaggc acacaccctc aaacctatca
     7921 tccaaatcgg gaaaaatgga ctcaacgacc aaatcaaaac cagcgtccgt caagctcttg
     7981 atgcgcgtga attaatcaag gttactctct tacaaaacac agatgaaaac atccacgaag
     8041 tagctgaaat tttggaagaa gaaatcggtg tggatacagt ccaaaaaata ggacgcatct
     8101 tgattttgtt taaacaatct agcaagaaag aaaatcgcaa gatttctaag aaagtcaaag
     8161 aaatctaaga ccgaaactcc aaataactgt ttttatagag aaataaaggg gactagccta
     8221 tgacaatcga actattgact ccctttacca aggtagagtt ggagccagaa atcaaggaga
     8281 aaaaacgcaa acaagttggg attttagggg ggaattttaa ccctgttcac aatgcccatc
     8341 tcattgttgc ggatcaagta cggcaacagt tgggactgga tcaagttctg ctcatgcctg
     8401 aataccaacc tcctcacgtt gataaaaagg aaaccatccc tgaacaccat cgtctcaaga
     8461 tgcttgagtt ggcaattgag gggattgacg gcctagtcat tgaaactatt gagttggagc
     8521 gcaagggtat ttcctacact tacgacacca tgaagatttt gacagagaag aatccagata
     8581 cggattatta ctttatcatc ggtgccgaca tggttgacta tctgcctaag tggtaccgaa
     8641 ttgatgaact ggttgacatg gttcagtttg tgggggttca gcgtccacgc tacaaggcag
     8701 ggacttccta tccagttatc tgggtggacg taccgctcat ggatatctcg tccagcatgg
     8761 tacgggactt ccttgcccaa ggttggaaac ccaactttct cctacctcag ccagtgctag
     8821 actacatcga gaaggagggt ctctactgat gtcttatcaa gactatatca actgctcccg
     8881 tgaggccttg ttgaaaaaaa tggcagagtt tctgcctgaa aaacgcttaa cccattgctt
     8941 gggtgtggag cgtgccgcta tggaattagc tcagcgcttt ggtgtagatg ttgagaaagc
     9001 aagtctagca ggtcttcttc atgactatgc taaaaagttg tcagatcagg aatttctaga
     9061 cttgattgac cgttaccagc tagaccctga cctcaaaaac tggggcaata atgtctggca
     9121 tggcatggtt ggaatctaca agattcagga agatttggat ttgcatgatt cagaaatcct
     9181 gcgagccatt gaagtccata cagtcggtgc tggtcagatg acagacctag ataaggttgt
     9241 ctatgtagca gactatatcg agcacaatcg atcctttcct ggagtagatg tggcgcgtga
     9301 gattgcaagt ctatcgctca ataaggcggt ggcctacgaa acagctcgta ccgtggagta
     9361 tctagctcat cagggattcc ccatctatcc ccaaaccctt gaaacctata acgcctttgt
     9421 gcactatttg aaagagaact aaatgaagac ggcttttata atcattgatg ttcagaatgt
     9481 tttagtagaa actgggttcc agacaaaaag tctattggga aaaatttctt atttacaaaa
     9541 ccaggctaga agcaagaata ttgaaattat ctatgttcaa catattgaga actctgaagc
     9601 tcaaacatca gaagattggc agttatctgc gcttttaaat cgaaaatctg atgaaaaggt
     9661 ctttcagaag aagtataaca gtattttcaa agaaactggc ttaaaagaat acttggataa
     9721 gcaggggatt gaaaaattag ttttatgtgg tatgcagaca gaatattgtg tggatacctc
     9781 tgtcaaggtt gcctttgaat atggctatca gcttattgtt ccagaaggtg ctgtcacaac
     9841 ctttgatggg gatgacattc cagcagaaac gattaatgaa ttttatgagg acatttggga
     9901 ggagcgcttt gcagatgtcc tagattacaa acatattttc taaaaagagg attaaatgaa
     9961 cgaaaaagaa ttactagaac tagtcgtgaa agcggctgat gagaaacgtg cggaggatat
    10021 cctcgcactt gatgtacaag atttgactag tgtgacggac tactttgtca tcactagctc
    10081 aatgaatagc cgtcagttgg acgctatcgc agctaatatc cgtgaaaaag tagctcaagc
    10141 aggctttaaa ggtagccatg tcgaaggcga tgcagctgga ggctgggtct tactggacct
    10201 cggtgctgtt gtcgtgcata tcttttcaga agaaatgcgt gcccattata atctagagaa
    10261 gctatggcat gaggcgaatt cagtagatat ttcagaagct cttgcttaga agatgaactc
    10321 agttgtagtc aactgggttt ctttgcttat tttagaaaaa ttgatagaaa ggtaaagggc
    10381 ctcgtgtttg tttcatttcg aaactgaaca cgagctaaaa gctgagataa aaagtaactt
    10441 tctttgtatc tgacgatagg gaagaaagtt tcctatgtac tctttgcttt taaggctctt
    10501 tgtatcttag aaaggatttg aacccgtcct gtaattctcg gaaaatagat agccttcctt
    10561 ggagctcggc tccatcgtaa ggctcctaat tttctatcga attacgggcg gacttggtat
    10621 catgttttat ggcgacttat gaaacctttg cggcggtcta tgatgcggtc atggacgata
    10681 gtttatacga caaatggacg aatttttctc tgcgtcattt gcctaagacc aaggagagaa
    10741 agaaactctt ggaattggct tgtgggacag gaattcaatc agtgcgcttc tctcaggctg
    10801 gttttgatgt gactggactt gacttgagtg cggatatgtt gaagattgcg gagaagagag
    10861 caacttcagc caagcaaaag attgctttta tagaaggtaa tatgctgaat ttgtccaagg
    10921 cagggaaata cgattttgtc acgtgttatt cggactctat ctgctatatg caggatgagg
    10981 tggaagtagg ggacgtcttt aaggacgtgt acaatgcgct gaatgaagag ggagttttca
    11041 tctttgacgt gcactcgacc taccagacag atgaagtatt ccctggctat tcctaccatg
    11101 aaaatgcgga agattttgcc atgctttggg atacatatga ggacgaagct cctcactcca
    11161 tcgtgcatga gctgactttc tttatcaagg aggctgacgg ttcctttagt cgccacgatg
    11221 aagtacatga ggagaggacc tatgagatct tgacctatga tatcttgctg gaacaggctg
    11281 gattcaagtc cttcaaactc tatgcagact ttgaggataa ggagccaaca gaaactagca
    11341 cccgttggtt ttttgtggcg cagaagtagg agattagcat gaccatcaca ggtattatcg
    11401 cggagttcaa tccttttcat aatggccata aatacctgct ggatcaggca gagggactga
    11461 aaattgttgc catgtcaggg aatttcatgc agcgtggaga gcctgctatt gtggataagt
    11521 ggacaagggc ccagatggcg ctggaaaatg gcgcagactt ggtagtggaa ttgccctttt
    11581 tagtcagtgt tcaggcagct gatttctttg gtcaaggagc tgtggatatc ttggatcgct
    11641 tgggcattga tagtctagtt tttgggacag aggaagttcg agattatcag aaaattgctg
    11701 acttatacac agagaaaggt gctgagatgg agaaatttgt ggaaaatctg cctgattccc
    11761 tttcctatcc ccagaaaacc caagctatgt ggaaggaatt tgctggtctt gatttttcag
    11821 gtaatacccc caatcatgtt cttgctctgg cctatgccaa ggcggttgca ggacgagata
    11881 tcaagctgca tccgattcag cgtcaggggg caggttacca ttctgtgaat aaagatgtgg
    11941 actttgcctc ggcgacagcc ctccgtcaac atcagaagga ccaagatttc ttagaacgct
    12001 ttatgccttc tgttgcccta tttgagcagg ccagtaaggt gatctgggaa gactattttc
    12061 ccttgctccg ctatcaaatc ttgtcaaatc ccgacctaac caccatctat caggtcaatc
    12121 aagaaatggc tgtgcgcatt aaagaagcta ttaaaacagc ccagtctgtg gaagaattgg
    12181 tcgagttagt gactaccaaa cgttacacca aggcgcgtgt cagacgccta ttgacctata
    12241 ttttggtgca ggttagagaa agtaacttgc cagaagcgat tcatgtcctt ggatttactg
    12301 aaaaaggcag gcaacatctc aagtctttga aaggtcaggt cagtctagtc agccgaattg
    12361 gcaaagaacc ttgggatgct atgacccaaa aagcagacca gatttaccaa ctaggaaagc
    12421 caagtatagc agagcaaaat tttggaagag tgccgattag aatagaaaca aactaagtct
    12481 accgaaaggt aggctttttg agatttttta cgaataatac tcttcgaaaa tcaaattcaa
    12541 atcgcgtcaa cgtcgccttg ccgtactcaa gtacagcctg cggctagttt cctagtttgc
    12601 tctttgattt tcattgagta taaatagata gaaaagaaaa tcttgtacaa gttcctgaca
    12661 atttctttct tttcgtgtat aatagtggaa aagagctaaa acgaggtatg gttatggaag
    12721 cagtccttta ctcaacattc cgaaatcatt taaaggacta catgaagaag gtaaatgatg
    12781 aatttgagcc tttgacggtg gtcaataaaa atccagatga ggacattgta gttctttcaa
    12841 agagcgaatg ggacagtatt caggaaaccc tgagaatcgc ccaaaataag gagttatcag
    12901 acaaggtttt gcgaggaatg gctcaagtcc gtgctggaag cactcaggtc catgtgattg
    12961 aggagtgatg aatgctactc aagtttacag aagatgcctg ggcagattat tgctactggc
    13021 aaaaccaaga taaaaaaaca ctaaaaagaa tcaataaatt aatcaaggat attcaacgcg
    13081 atccatttac aggaatagga aaaccagagc cactcaaata tgattaccaa ggagcctggt
    13141 cacggcgtat tgatgcagaa aatcgcttga tttatatgat ggatggagat agcgtggctt
    13201 tcttgtcctt taaagatcat tactaagtct attgaaaggt aggctttttc tcaagtctct
    13261 actaaactac tatttctaaa caacaagttt attttggtaa atgtgttcca aattccccaa
    13321 aacatattct attttagg
//
LOCUS       DAVDWT010000046        13091 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVDWT010000046 DAVDWT010000000
VERSION     DAVDWT010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13091
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.046"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(170..1372)
                     /gene="rpsA"
                     /locus_tag="VUW50_001838"
     CDS             complement(170..1372)
                     /gene="rpsA"
                     /locus_tag="VUW50_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET7983682.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSIKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRVARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1534..1605)
                     /locus_tag="VUW50_001839"
     tRNA            complement(1534..1605)
                     /locus_tag="VUW50_001839"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1571..1573),aa:Gln,seq:ttg)
     gene            complement(1615..1695)
                     /locus_tag="VUW50_001840"
     tRNA            complement(1615..1695)
                     /locus_tag="VUW50_001840"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1659..1661),aa:Tyr,seq:gta)
     gene            complement(1745..1975)
                     /locus_tag="VUW50_001841"
     CDS             complement(1745..1975)
                     /locus_tag="VUW50_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HET7983683.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(2143..2787)
                     /gene="pcp"
                     /locus_tag="VUW50_001842"
     CDS             complement(2143..2787)
                     /gene="pcp"
                     /locus_tag="VUW50_001842"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET7983684.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(2802..3725)
                     /locus_tag="VUW50_001843"
     CDS             complement(2802..3725)
                     /locus_tag="VUW50_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HET7983685.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(3722..4411)
                     /locus_tag="VUW50_001844"
     CDS             complement(3722..4411)
                     /locus_tag="VUW50_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HET7983686.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(4476..5180)
                     /locus_tag="VUW50_001845"
     CDS             complement(4476..5180)
                     /locus_tag="VUW50_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7983687.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSEITDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGFPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(5236..6258)
                     /locus_tag="VUW50_001846"
     CDS             complement(5236..6258)
                     /locus_tag="VUW50_001846"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HET7983688.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(6390..8861)
                     /gene="parC"
                     /locus_tag="VUW50_001847"
     CDS             complement(6390..8861)
                     /gene="parC"
                     /locus_tag="VUW50_001847"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET7983689.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEYK"
     gene            complement(9281..11224)
                     /gene="parE"
                     /locus_tag="VUW50_001848"
     CDS             complement(9281..11224)
                     /gene="parE"
                     /locus_tag="VUW50_001848"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HET7983690.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            11360..12001
                     /gene="plsY"
                     /locus_tag="VUW50_001849"
     CDS             11360..12001
                     /gene="plsY"
                     /locus_tag="VUW50_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET7983691.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDFLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(12044..13091)
                     /locus_tag="VUW50_001850"
                     /pseudo
     CDS             complement(12044..13091)
                     /locus_tag="VUW50_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3732 a         2950 c         2288 g         4121 t
ORIGIN      
        1 aactttgaaa acttcaaaaa acggattttc atcgctctga atatcaaaaa agaaaggacg
       61 aaatttgtcc tttctcgagc ttagcttttt ttcaacccac tacagttgac aaagagccag
      121 aaaaatcctt tgtttagtaa acaaaggatt ttgtgaattt tcaatttgat taaagttcga
      181 tatcaccaaa caaatcagcc attgaaaatc ctgtttgtgt ttctggaagt tcgaaatcac
      241 gcttttcttg acgtcttgga cgacgtggac gagcaacacg tttttcttct ttttgtcctt
      301 cttcttgggc tggacgttct tcaagagctt taatagaaag tgacacgcgt tctgcatctg
      361 cgttaacttc aagaacttta acttgaactt cttgaccaac tttaagagct tcttttggat
      421 tttcaatccg tttgtgtgaa atttgtgata cgtgaacaag tccatcgata cctggcaata
      481 cttcaacaaa tgcaccgaag tcagtcaaac gtttaactgt tccttctact acatcacctt
      541 tagccaattt ttgctcaacg ccatcccatg gtcctggtac tgttgcttta atagaaagtg
      601 atacacgtcc ttcttcttcg ttaagatcaa ggattttcac ttcaatttct tcaccaacag
      661 ttacaactga ttttggtgat acgttacgtt catgtgacaa ttcagtcaag tgaaccaatc
      721 catcaacacc accaaggtcg atgaaagcgc cgaagcttgt gatacgagca actttaccag
      781 ttacaacatc accaacagcc aatttaccga atacttcagc gcgagctgct gcagtagctg
      841 cttcaacaac ttcacgacgt gaaaggatga agcggttttc tttagcgtta acttctttga
      901 ttttagtatc aaattcttga cctacaaaac gctcagcgtt acgtacgaaa cgagtatcca
      961 acatcgaagc tgggataaat ccacgaacac cttcaaattc tactgaaagt ccacctttaa
     1021 cggcacgcgt tcctttaaca gtaacaactt cttcttcgcg accaacaagt ttgtcccatg
     1081 ctttgcgagc ttcaaggcgt tttttagata caaggtatgt aactgtatca gtatctttac
     1141 caactacttg acgaagtaca agaacatcca atacttctcc tactttaaca aagtcattga
     1201 tatctgcatc acgatcgttt gtcaattcgc gaagagtcaa gacaccttca acaccagttc
     1261 cagagattgc aacgttagct tgagtcgcat caactgtcaa tacttcagca ctaacaacat
     1321 caccagtctc aacttgacta acgctattta gcaaatcttc aaattcgttc atctaaaaaa
     1381 tcctccaaca atcaagtttt tcttaaactt gacatactta taattttttt cctaagcacc
     1441 cgcaaggaca tgttcatatc attcacgtct ttcaattata aaaataaaat accctaagat
     1501 attttgcttt ttatcttgat tattacctaa gaactggggt agctggattc gaaccaacgc
     1561 atgagggagt caaagtccct tgccttaccg cttggctata ccccattaat gaaatggaga
     1621 gagagggatt cgaacccccg aacccgaagg agcggattta cagtccgccg cgtttagcct
     1681 cttcgctatc tctcctacaa tcaacatgga ctattatatc atgaaaattt caaaaaaaca
     1741 agacttattt tgctaaatta taattttcaa tcaaaatttc cacagctttt tccaattttt
     1801 tataaaaact atcgacattc ccattcttta taatggcaaa ttgtccgtct aattcggcaa
     1861 tttctccgat aactttttta ccgatagtca atgtataacc ttcaaaactg tctttaccaa
     1921 cattaacttt ggcatccgct acttgaattt cgattttttt atctttcttg ctcatttcat
     1981 acctctcttc actttttcta ttttacaaga aaatcctaaa actagcaaga aaaaactcta
     2041 tatcaggctt ctctgatata gagttttatg taaacaacct caattgattt agcatagtta
     2101 atagttagta aacaagcata attgactatc ctttttaggt tatcagtgag tctcaccgcc
     2161 tactaactgc aaatccttat ctttgtagtc tacaatagct tcaatagcta cctctatgcc
     2221 tcttacaata tcacatagac tcattcctgc agtattcggc ttatttacta cttgttccat
     2281 catatatgga atatgcatga aacctgctct catattaggg aattttttat ccactaaata
     2341 aagagcttgg tacatcaaat gattgcaaac aaaggttcct gcactattgg atacaactgc
     2401 cggaagtccc tgttttttga tagcttgtac catcgctttg ataggtaaac tactaaaata
     2461 ggccgatgct ccatcaatac gaatcggtgt atcaattggt tgattgcctt cgttatcagg
     2521 tatgcgagca tcatcttgat taatagctac tcgttcaggt gttaagccgg tcctgccgcc
     2581 tgcttgtcca atacaaagta cagcatctgg ttgatatcgt aatatttctg cctctaaaac
     2641 ttctgacgac ttataaaaaa ccgttggaat ttctacccag cgaacttcag ccccattaat
     2701 ctcagatggt aataatttta cagcctccaa agctggatta atcttttcac ctccaaaagg
     2761 attaaaacct gtaaccaata ttttcatttt attttccttt actaaaatgc gagaaagtac
     2821 attaagaata tgtgaataac aatcattact agagcaacac ctgcttgagc ctttataacg
     2881 ccattctgat ctttcatatc catcaatgct gctggtagag cgttaaaatt agcagccatt
     2941 ggggtcaata aggtcccaca ataacctgct gtcatggcaa gagcaccagc cacaattgga
     3001 ttagctccca gagcaaatac aaagggaact ccaacacctg ctgtaataac ggtgaatgct
     3061 gcaaaagcat ttcccataat cattgtgaat agaaccattc caagaacata ggccaaaact
     3121 cctataaagc gactatctga aggaacaata ccgctaatca gatgagagat aacatcacca
     3181 acacctgcta cagtaaaaat agcccccaaa gcccctaata attgaggaac aataccactt
     3241 gttgaaactt gctgagtcat tcgattattt tctgataaca gactcttagg gtgactattg
     3301 gtaatcacaa gaacagaaat tgtagcaaac aaggcggcaa ggctaatcga aatcttgcta
     3361 aattctggaa tcatttgcgc taagaccaac gcaagtattg ccatcagcat aactggaata
     3421 aaaattttat ttttcaacct gttagattca atattggctt tcatttcatc taaggatggc
     3481 aaggttccga tacggacttg cttaaacaat gttaacagcg ataataggat tacaataata
     3541 ccaatactca tatttggcat ataggaacca cctataaacg taatagacaa tagagtccaa
     3601 aatgcagatg tcccaagtcg aactgggttt gttttatctt tataactaca ataggctgta
     3661 tggagaaatt gacaaccaat cacaatatag gtcaactcta atagttgctt tgccaactct
     3721 gtcatttttg ttctcctccc ctagtctttt ttgatatcaa ttttttatca aataaataat
     3781 tgtaaatccc cactacaata agtgttataa cagcaacaat aatagatgta gaagcaatcc
     3841 ctgcataatt gctttcatag cctaactgat ctaatgttcc ccctatcaag aggactcccc
     3901 cagcacctac aaacgtattt tgagcaaaga aatttccaaa attttcattc gcagccgcac
     3961 gcgcttttat tgtctcatct tcaacctctg ttaactttct acctaattga gactctgcag
     4021 ctgcttctcc cataggctga accaaaggtc tgacaaactg agggtgtcct cctagacgaa
     4081 ttgaaaagaa accagctaac tctcgaataa agaaataaac tgtatagaag tttccaactg
     4141 tcagaccttt aatctttcga atcaaatcga ttgatcgttg cttgagtcca aaggtttctg
     4201 acagccccac aagaggcaag gtaaccataa aaatcgtgag cactcgctga ttgctaaatt
     4261 cttttcccaa aatctccaaa aattcaacga gagaaacacc tgaaactaaa gctgtaacca
     4321 aaccagctaa gactactgtt gcaattgtat caaattttaa aataaaaccc acaacaatga
     4381 ttgctattcc tattaatcta atccactcca tatcaaactc ctttatattc aaaatgacag
     4441 tattttaaaa attttatcaa gatcaatacc attccttatt taatgtattt ttctagttct
     4501 ttttggtatt tgctattgga ttccaatttt tctttttgcc attttttaaa aacctcgtta
     4561 tattcttttg ttgtaacaat atctttttgc aatttcattc ctttaaagat atatggttcc
     4621 cccttaatac caacttgtga gtatggtttt gagaatggta ctacgttact tacaactgga
     4681 aaaccaccag atgaagctgt tggcatcaat aatgaactat ctgtcgacca agcttgagct
     4741 ttggcatatt tttcatatct cttctctagg tcagtgatct cagaaacagc atcttctaac
     4801 aatttcttat atttatccaa accaggttta gctacaacat ccttatcttt tcctttcgta
     4861 atgccaaggt gtttcatggc agaaccagat tttggatcca taatattcaa gtaagacgct
     4921 ggatcttgat agcttggagc ccatcctgta ctgttcaaat catagtcttt ttgagaagga
     4981 gcaacattgc cgtatttatc attttccatc aaaccatcaa taacatttcc aataacgtct
     5041 gtcctcgatg ttcgagtcgc tatactgtag cccaatgatg ctggatctac tgcatagaca
     5101 taagaaaatg ttgtcggtgc atctgcttct ttatcagttt ttccacaagc cactaaaata
     5161 gctgacgtgc tcaggaccac tcctgctgtt aagagccact ttttctattt cataaaaaat
     5221 ctcctttggt ttattttaat ctacttttac aatccaacct tctggcgctt caatatcgcc
     5281 aaactgaata cccgtcaatt cattatataa tttacgcgtc acaggaccta cttctgtttc
     5341 actatagaat acatggaaat catcaccatg ttgaatacct ccaattggag aaataaccgc
     5401 tgctgtacca caggcacctg cctctacaaa acggtcaaga ttatcaattg gaacatcacc
     5461 ctcaatagga gttaatccca agcgatgttc tgccaaataa agcaaggaat acttggtaat
     5521 agatggcaag atagatggac tcaatggtgt tacaaattca ttatcagctg taattccaaa
     5581 gaagttagct gatccgactt cttcaatctt tgtatgagtt gatgggtcca gatagataac
     5641 atctgagaaa tgacgtgact tggccatttt tcctggtaag agacttgcag catagtttcc
     5701 accaacctta gccgcacctg taccatttgg tgctgcacgg tcgtactcat cctgaatcaa
     5761 gaagttggtt gggaccaaac cacctttaaa gtaatttcca actggcatag caaagatggt
     5821 gaaaatgtac tcttctgccg gttttacccc gataatatct ccgacaccaa tcaaaagagg
     5881 gcgaagatat aaggttccac ctgttccgta tggtggtacg tattcttcat tcgcacggac
     5941 aactgcttta caagcttcta caaacatgtc tgtcggaact tgtggcatca agagacggtc
     6001 acatgtacgt tgcagacgtt tagcattttc atcaggacgg aacagttgaa cactgccatc
     6061 cttagtacga taagctttca aaccttcaaa tgcttgttgt ccatagtgaa gacttggaga
     6121 agactctgaa atatgcaaag ttgcatcctc tgtaagctct ccttgatccc attgtccatt
     6181 tttgaaatga gcaagatagc gataaggtaa tttcatatag gaaaaaccga ggttttccca
     6241 atcaatcgtt actgtcatat tccactcctt attctaaaaa cctatttctt atattctaca
     6301 ctatttttct aaaatagcaa gtatattttg taattttcag aaaatttctc caataaaaac
     6361 cagctcttag aacttattct tcatttcact tatttatatt cagtaactac ttcctgaaga
     6421 taagcgtcaa aaacttcttc atctgaaatc gtgtcagaaa tgaagcttcc attgctagtg
     6481 cgttctgaca agttcaagtc ttgcaatcgg ctttcataga ttgttccttt attggattgg
     6541 acaagcagag tttggtcgtt cacatccact tccgtactga agaaatcgcc aacaaatcct
     6601 tgctctgcaa ctgctcctgc caagaagaca cgatgcggtt tgtttttcaa ctcacgcaag
     6661 acttgtaatc ctcgtttggc acggctggtt gctagaattt cctcaataga aacacgtttc
     6721 aagcttccac gctgggtcaa gaggtagaag gacgaagtat tacagataaa gccagattgg
     6781 aggacatcat cttctttcaa attcatagcc ttgacacctg ctgccttagc accgacaacc
     6841 ggaacctctt cgatattgaa acgcagggca taaccatttt gactaaccaa gacaacatca
     6901 tctagtttaa tcggagccac tgctacaatc tgatctgtat cgtctttgag cttagcatac
     6961 ttgacagact tagatctata ggtccgccat ggagtgaatt cttttcgctc tacccgtttg
     7021 atttgaccaa ggcgagtcac tgcaaagtag gttgtcgcat cgtcaaactg atccagtact
     7081 tccacataaa ggatttcttc attcgtttca aagtttgtga tggtttggct cagatgctct
     7141 ccgatgtcct tccaacgaat atctgccaac tcatggattg gtctgtagat gacatttcca
     7201 agacttgtga acatcaagag gtgctgggtt gtcttggcag attgaacaaa aatcaaacgg
     7261 tcatcatcac gcttgccaat ttcttccaag gtggaagccg caaaggaacg tggactggta
     7321 cgcttgatgt aacctgcctt ggtcacgctg acgtaggtat cttcctcagc gataagacta
     7381 gctgtatcaa tctcaattgc tttcgcagtg tcttctaaag aactcaaacg aggagttgca
     7441 aatttcttct tgacctcacg aagttctttc ttcatgagat tgtacatagt cctttcatca
     7501 ccgataatag ccgccagcat agcaatcttc tcacgaagct ctgcttcttc ttcctgcaag
     7561 acaaccacat cggtattggt caaacggtac agttgcaaag ttacgatagc ctcagcctgt
     7621 tcttccgtaa aatcatagct aactttgagg ttttccttgg cgtccgcctt attctcagaa
     7681 gcacggataa gagcaatgac ttcatccaaa atcgaaatca cacgaatcaa accttcgacg
     7741 atatggagac gtttctcagc cttttctttg tcaaagcgtg aacgcgccaa aatcacttct
     7801 cgacggtgag cgatgtagct agacaggatt ggaacaatcc caacctgacg aggtgtgaaa
     7861 ttgtcaatcg ccaccatatt aaagttgtag ttgatttgta ggtcggtgta cttaaataag
     7921 tagttgagaa caagctcagt attagcgtct ttcttaagtt cgatagcgat acgaagacca
     7981 tcacggtcag actcatcacg aacctcagca atcccagcta ccttgttatt aacacgaaca
     8041 tcatcgattt tcttgactag attggcctta ttgatttcat aaggaatctc agtaataacg
     8101 atttgttcct taccaccttt tagcttttca atttcagtct tggaacgaac aaccacgcgc
     8161 cctttcccag tctcataagc tttcttgatt tcatcacgac cctgaataat agcccctgta
     8221 gggaagtctg gtccaggtaa gaattccatg agtttatcaa tctttgcagt tgggtggtca
     8281 atcatgtaaa ctgcagcatc tatgacctca gctaaattat ggggaggaat gtctgtggca
     8341 taaccagccg aaatcccagt cgaaccattg accaagaggt ttggaaaggc tgctggcaag
     8401 accgttggtt ctttctccgt atcgtcaaag ttccatgcaa aaggaactgt ctttttctcg
     8461 atatcctgaa gaaggtagcc tgcaatttca gacaaacgtg cctcagtata acgcatagcc
     8521 gcaggaggat ctccgtccat agaaccgtta ttaccgtgca tttcaactag aatctcacga
     8581 tttttccagt tctgtgacat acgaaccatg gcatcataga tagaagaatc cccgtgtggg
     8641 tggaaattcc ccatgatgtt cccgactgac ttggccgact tacggtagct cttgtcaaaa
     8701 gtattgctat ccttattcat agaataaaga atacggcgct gaaccggctt caacccatca
     8761 cgaatatctg gcaaagcccg gtcttgaata atgtacttgg agtagcgacc aaagcgctct
     8821 cccatgatgt cctccaggga catgttttga atgttagaca taagatacaa agcccataaa
     8881 ataccaagtg aaaatagaaa attcttgaag taagcaaact cacaagagaa tttatctttt
     8941 tcacacagta tctagggcgt gttcaactcc tttcaaagaa tgtagagtag gtttttatgc
     9001 agtaaaagat attttacggg aattcctccc gtgttcagtt acgataagta accaaactat
     9061 cctgtttgta tttttcaata tgaaaatctg gttttccaaa attagtctta gtttgtgtct
     9121 tagccgctcc cttaagcgcc tctttgagat aagcactcat agcagattct tcattaataa
     9181 tcctgcaatt ttttcaaacc aagattttca aactgctttt tcacatagtc attcacatcc
     9241 gactctaatt tccagtttac taacatatta ttttctttca ttaaaacact gtcgcttctt
     9301 ctagcgtaaa cttgacatta tcttcaatcc atttacggcg tggttctacc ttatctccca
     9361 tgagaacatt gacgcggcgt tcggcgcgcg ctaaatcttc aattgtgaca cggatgaggg
     9421 tacgtgtttc tgggttcatg gttgtttccc agagctggtc cgcattcatc tcaccaagtc
     9481 ctttgtatcg ttggagggta gcgcctttac cgaactgttt acggagttct tctagttctc
     9541 cgtccgtcca agcgtaggcc acttcttctt tctttccttt acctttggac atcttgtaaa
     9601 gaggtgggag ggcaatatag acatgacctg cctcgactag cggacgcatg taacggtaga
     9661 aaaatgtcaa gagcaaggtc tggatatggg caccgtcggt atccgcatcg gtcatgataa
     9721 tgatcttatc atagttggca tcttcaatag agaagtctgc tccaacaccc gcaccaatgg
     9781 tataaatcat ggtattgatc tcttcatttt tgaggatatc cgccatcttg gccttggctg
     9841 tattgataac cttaccacga agaggtagaa tagcctggaa cttgcggtca cgaccttgtt
     9901 tggcagaacc accggcagag tccccctcaa ctagatagag ttcattctta gcaggattct
     9961 tagattgggc tggggtcaat ttcccagaca acaagccctt atctttcttg tttttcttcc
    10021 catttcggct ctcatcacgc gccttacgtg ctgcttcacg agcatcacgg gccttgatag
    10081 ccttgcggat gaggttagaa gctaattccc cattttccat aaggaaaaag gtcaacttat
    10141 cagccactat tccatccaca actgggcgag ctagggggct tcctagttta tccttggtct
    10201 gtccttcaaa ctgcaagtgt tcttcaggaa ctaagataga aagaacggcc gctagtccct
    10261 cacgatagtc tgaaccttca aggtttttat ctttttcctt gagaagacct gttttacgtg
    10321 catagtcatt catgaccttg gtaatggcag acttgagtcc tgtctcgtgc gttccaccgt
    10381 ccttggtgcg aacgttattg acaaaggata gaatgttatc tgagaatccg tcattgtact
    10441 ggagggctac ttccacttga aaaccattgt cttccccttc aaagtaaaga actggcgtca
    10501 agatttcctt atcttcgttg agataagaaa caaaatcttg tactccattc tcatagtgga
    10561 actcaatcgc ttcatctgtt cgcttgtccg ttaaagacaa ggtcacattt ttcaagagaa
    10621 aggctgattc attaaggcgc tctgaaatgg tattgtactt gaaatctgtc gtagaaaata
    10681 tagtcgcgtc aggcataaaa gtaactttgg tgcctgtttt agacttgggt gctgtaccga
    10741 ttttcttcaa agtcgtgaca ggttttccac cattttcgaa acgttgcttg taaactgcgc
    10801 catcacgggt aatttcaact tctaaccagc tagaaagggc gttaacaacg gaagaaccca
    10861 ctccgtgaag tccacctgat gtcttatagc caccttgacc gaatttccct ccggcatgaa
    10921 gaatggtaaa gataacctca acagttggaa ttcccatagc gtgcatacct gtcggcatcc
    10981 cacgtccatg gtcttgaacc gttagactac cgtctttatt gatagttaca tcaatacgat
    11041 caccaaaccc agacaaggct tcatcgactg cattatcaac gatttcccaa actaggtgat
    11101 gaagaccagc gccatcggtc gatccaatat acatccccgg acgttttcgg accgcatcca
    11161 acccttctag cacctgaata gcatcatcat tataattgtt aatattgatt tccttttttg
    11221 acacaaggaa cctcctattc gttcatcttt actattctac aggttttcca aggattttgc
    11281 aaaatttttc tttctccgat gtgacaattt cagcagagat tctctgcttt tctttcccaa
    11341 ttcatgatat aataggagta tgattacaat agttttatta atcctagcct atctgctggg
    11401 ttcgattcca tctggtctct ggattggaca agtattcttt caaatcaatc tacgcgagca
    11461 tggttctggt aacactggaa cgaccaacac cttccgcatt ttaggtaaga aagctggtat
    11521 ggcaaccttt gtgattgact ttttcaaagg aaccctagca acgctgcttc cgattatttt
    11581 tcatctacaa ggcgtttctc ctctcatctt tggacttttg gctgttatcg gccatacctt
    11641 ccctatcttt gcaggattta aaggtggtaa ggctgtcgca accagtgctg gagtgatttt
    11701 cggatttgcg cctatcttct gtctctacct tgcgattatc ttctttggag ctctctatct
    11761 tggcagtatg atttcactgt ctagtgtcac agcatcgatt gcggctgtta tcggggttct
    11821 gctctttcca ctttttggtt ttatcctgag taactatgac tttctcttca tcgctattat
    11881 cttagcactt gctagtttga ttatcattcg tcataaggac aatatagctc gtatcaaaaa
    11941 taaaactgaa aatttggtcc cttggggatt gaacctaacc catcaagatc ctaaaaaata
    12001 aaacgccagt tctgtactga ccccaaacag ttagacaatt aatttatccg aaggatttag
    12061 ttctgtattg cacagggcta agtcctttta gttttatctt aattcgttta ttgttgtagt
    12121 aatcaatata gtctataatg gcttgttcca attgcttaag cgactgaaac gacttctcat
    12181 aaccgtaaaa catttccgat ttcagaatcc caaagaagga ctccatcata ccattgtctg
    12241 ggctgttacc cttacgtgac atggatgctt gaactccctt actctctagg aaccgatgat
    12301 aagaatcgtg tcggtattgc cagccttggt cactatggag aatcgtattc tcatagtatt
    12361 tctctgtaaa ggcctgttct aacatactct tcacttgttc taagttgggc gaagttgaaa
    12421 gattataggc gataatttcg ctattaaagc catctaaaac tggtgataag taaagctttt
    12481 gagtacttgc tggaatggca aattctgtca catctgtgta gcacttttcc attgttttag
    12541 agccttcaaa ttggccttga atgagattct ctgccttctt accaacgtct cctttatttt
    12601 cgtttctgtc gcattttagc ttgtaaattg agtactttca tcaagccttg aactctttta
    12661 tgatttacca gataaccacg atttcttagt tctaaataaa ttcgacgata accataattt
    12721 cccttgtgtt cgataaaaat ggattgaatt tcagctttaa gctcttggtc cttatctggt
    12781 ttgtctaact gtttcaagtg atagtagtag gtccaacgag ctagcttaat ggcttttaga
    12841 agaatatcta acgaaaactc agtcattaat tcttgaacaa tttctgtctt tcttctttct
    12901 ctttttcctc cttcaatcgg agttctctta acttttttag gatggcattc tccgctctca
    12961 ggtactcatt ttctgcttga agacgttcta attctgtcct ctcttcaggt ctcgttcttg
    13021 gcttacgtcc cattttaggt actctccctc ttggtttctc aacaatagta tacccgtttt
    13081 ttctgtattg t
//
LOCUS       DAVDWT010000047        12685 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVDWT010000047 DAVDWT010000000
VERSION     DAVDWT010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12685
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.047"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(221..829)
                     /locus_tag="VUW50_001851"
     CDS             complement(221..829)
                     /locus_tag="VUW50_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HET7983692.1"
                     /translation="MQIKFIDKVSNLIMLNLLYVASVVTVIAIGSGESALIATLIKIV
                     RHEESYPYRDFANSFFKDYWKNLGAALISNLPILILLFSFFFLPYIPLPIYIISILRH
                     IGVIYIILHLMATTFLIPLIGRYNNTLKNSLHNSIMLAYKHFFIAVLIRIIEIIPVLL
                     FFILQNQLLVWITLMIFILPSITKYANAFLYNFIFSKYEKLN"
     gene            995..1165
                     /locus_tag="VUW50_001852"
                     /pseudo
     CDS             995..1165
                     /locus_tag="VUW50_001852"
                     /inference="COORDINATES: protein motif:HMM:NF013577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            1154..1765
                     /locus_tag="VUW50_001853"
     CDS             1154..1765
                     /locus_tag="VUW50_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET7983693.1"
                     /translation="MTKDILELESQKMSSDTFIDEIKNNYLSIVESTRKLIDGRQIEL
                     AIKLIREANQILIIGVGSSGNAAREFESSLLRIGIISKTVIDTHFQLMHTALLKDNDL
                     IIAFSLSGSTKEVEETLLNAKRKNVKIISITNYSSRNIAKLSDCVLLTSKKESYLEGG
                     SLMAKASQLFIIDVICTRLSLINYEDTICKKEEIASLLSNKVE"
     gene            2482..3168
                     /locus_tag="VUW50_001854"
     CDS             2482..3168
                     /locus_tag="VUW50_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET7983694.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKAAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMADISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPL
                     EIAKKFIEVV"
     gene            3169..4101
                     /locus_tag="VUW50_001855"
     CDS             3169..4101
                     /locus_tag="VUW50_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7983695.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSPFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFSFPAPL
                     IFALILNEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGIINSLLSSLGMEP
                     ISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTLYEAADMDGANRWHKMWH
                     VTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVSDVLSTYVYRQGILNSDF
                     SYSAAVGLFNNIVALLFVLLANKIAKKLGEEGIV"
     gene            4112..5041
                     /locus_tag="VUW50_001856"
     CDS             4112..5041
                     /locus_tag="VUW50_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7983696.1"
                     /translation="MIFSKNTKKSNRDKETFVDYLFIFILIIITLLMLYPLWWVVMTS
                     LSEPVWLSKNVVKLFPLKIDITAYKFIFGDSKLWQAYGNSVLYAALSTIVSVAVCSMY
                     AYPLTIPEIRGKKFWNTLILIPMFFSGGMIPLYIQVANLGLLNTAWALVLPGAVSTYY
                     IILFRTQFKSIPMELRESARIDGASEFRIFWNLVLPLSKVIFMIIALYCIVGSWNSFT
                     PPLLYLNDDTKMPLSIYLRNLVVTNTTDTAELSLSAGNTYYQQIAAQGGGIGMRIAIK
                     MGTIVVSVIPIMMIYPFIQKHFVKGILLGSVKG"
     gene            5055..6494
                     /locus_tag="VUW50_001857"
     CDS             5055..6494
                     /locus_tag="VUW50_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983697.1"
                     /translation="MQNLKKYLLLDSGLVVGYTLENAQLKLNKLKKDTENNPLFGEEY
                     FAENPKLWEVRFDNSYPNVIYDKELKLYRCWYQTFVSDEASEETPLAERGEKEYIVKS
                     SRMTALCYAESKDGVKWEKPNLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRY
                     KLVSKLEFSPNLHYMVVVFSEDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVIT
                     RTWANNVRLSAITFSDDFINWSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHE
                     GDTLNENYDKVDLELKFSANLEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPI
                     IEDDKIWFYYMGGNGNHTGFRESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYD
                     DEIYILADIPENGYVKVAVGTKYGNAYSDFGQEDISLVQVSDGKYKLEFNKKSILDLG
                     NSPVAFKFEIENAKLYGIEGSIQQHYLKY"
     gene            6563..8140
                     /locus_tag="VUW50_001858"
     CDS             6563..8140
                     /locus_tag="VUW50_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7983698.1"
                     /translation="MVKLNKVLKGSVALLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDQTLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSKHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKSFDWQPLVYPEINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEELDVLTKSEAGLNTFFDENIIKFIEGQRDMSEWDSFINEAKSKDLDK
                     VTKVYNDAYKRVKDRMK"
     gene            8203..9663
                     /locus_tag="VUW50_001859"
     CDS             8203..9663
                     /locus_tag="VUW50_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7983699.1"
                     /translation="MYDVVVIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIEKGGTVFSSMEELMNVSDFYQITIPYQPE
                     VYESVLTEMLNESNVDILLNVEINNIVFDNNKIHSVEIISNQEVNYLESKVFIDATGN
                     ATIASASGANVYEKNSSYGCLMRVGGVDITKTLEHIEKTKQWEFEEGYNEWLDNVLAN
                     RKYRATGAVLRDPVSYDHAPMLTKDDHFMNEEKWSYIKERWERFGFIYTLEVSLFRNE
                     LREAVEAGDFEFEIYHDDESGVTMNGDGISYGSWGQNVALINVAKAFGFNPKYMEDES
                     HANILSRKYNLTFLSFLKKYVPGFENSFILDQGTRATNRSCRHIDNSSMMDEILDFPM
                     YTFKTMYSYQTPKEITYKSIVGGKLNNLFVIGKGAYQSESFRSQISCMLMGISSAAAA
                     KTIIEDKSNTLNINRDLFKSNLDILFTWEFSNWITD"
     gene            9685..10140
                     /locus_tag="VUW50_001860"
     CDS             9685..10140
                     /locus_tag="VUW50_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET7983700.1"
                     /translation="MNDTNKNQYFVYEFDTSFVEMSYSQFFEGYCILFSKEPYPSLSD
                     MPMEFRKEFLMEMSILGDAMLEVLNAYRINYDILGNSTPVLHAHLFPRYMWEEEERRK
                     TVVHRYDSSVFSNPEFEFTIEKHGPIIKKLRSAIDRHYREYQQLLDDSN"
     gene            10645..11094
                     /locus_tag="VUW50_001861"
     CDS             10645..11094
                     /locus_tag="VUW50_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET7983701.1"
                     /translation="MELVEFKNGHVNTVHEHIAITKVKEFLNNHDIPKDKGGIHKIST
                     DFFYNIIDMETTTEENRPWESHKEYYDVHYLLNGEEIILYNFLSQMELSEYKVDDDWQ
                     QMNGTALFSIKLKKDMLLLLEPNDAHKTGLLVEEPINIKKVVFKVKI"
     gene            complement(11139..11833)
                     /locus_tag="VUW50_001862"
                     /pseudo
     CDS             complement(11139..11833)
                     /locus_tag="VUW50_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            12284..12685
                     /locus_tag="VUW50_001863"
                     /pseudo
     CDS             12284..12685
                     /locus_tag="VUW50_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         4448 a         1683 c         2299 g         4255 t
ORIGIN      
        1 tttttggggt cagtacagta ttaaatacca tgatcttatg cgttttataa taaaaaatct
       61 gttcttgttt tatttttaga aaatttacta aagactaaaa atattatgaa taatttaaaa
      121 aatagtcagg ttatatagtt ttttgaaatt aaaattattt tatcgttgaa attagttaaa
      181 aatttttata aaatcaagat gcaaggctat gagatgttct ctagtttaat ttttcatact
      241 tcgagaatat aaagttatat aaaaatgcat tagcatattt tgttattgag ggaagtataa
      301 aaatcattaa agttatccaa actagtaatt gattctgtaa tataaagaag agtagaactg
      361 ggataatttc aataattcta ataagaacgg caataaaaaa atgtttatag gctaacatga
      421 ttgaattgtg tagagaattt tttaaagtgt tattatatct accgattaaa ggaattagaa
      481 atgttgttgc cattaaatga agtatgatat atattactcc aatgtgtcgt aagatagaaa
      541 tgatatatat aggtaatggt atgtagggta ggaagaaaaa actgaataat aagattaaaa
      601 ttggtaaatt agatattaat gctgctccta aatttttcca ataatcttta aaaaaactgt
      661 ttgcgaagtc tctataggga taggattcct cgtgtcgtac aatttttatt agagttgcta
      721 ttagggcact ttcacctgat ccgatagcaa taactgttac tactgacgct acatagagta
      781 gatttaacat tataagattt gaaactttat caataaattt tatctgcatc tttaactcct
      841 gctgttttta tacttataat atcacatcat ttgagtttca tcaactttga atacattaag
      901 aaagaatata actatgagga aaggaaaaaa ttattatgaa taaatccaaa aaatataaag
      961 aaagaattca ttctgaatac gataatctta ctaaatcaga gaaaaaattg ctgactatat
     1021 tttagttaga gatagtgaaa tagtttatga aacgtcttca gaaattgtta gtaaagtaaa
     1081 agttggagag ggaactctta atcgattttg caaaaagatt ggatactctg gttttcagga
     1141 actaaaatta aaaatgacga aagatatatt agagttagaa tctcaaaaaa tgagtagtga
     1201 cacatttatt gatgaaataa aaaataatta tttatctatt gtggaaagta caagaaaatt
     1261 aattgatggt agacaaatag aattagctat aaaattaata agagaagcaa accagatttt
     1321 aattattggt gtgggttcta gtggaaatgc ggctagagaa tttgagagta gtttgttaag
     1381 aattggaata ataagtaaga cggtaattga tacccatttt caactcatgc atactgcgtt
     1441 attgaaagat aatgatttga taatagcgtt tagtttatct ggttccacaa aagaagttga
     1501 ggaaacttta ttaaacgcaa aaagaaaaaa tgttaaaatt atttcaataa caaattattc
     1561 gtccagaaat atagcaaaat tatctgactg cgttcttttg acatcaaaaa aagaatctta
     1621 tttagagggt ggtagtttaa tggctaaggc ttctcaacta ttcataattg atgtaatatg
     1681 cactagactc tctttaataa attatgaaga tactatatgt aaaaaagaag agattgcttc
     1741 tttgctgtca aataaagtgg aataaatcaa taatttttaa attttaataa aaatttttta
     1801 aaataaatta aaaaacgatc ctaaaagggt cgtttttttg ctaaagtttt aaaaataaac
     1861 aataattaag attatagtgc gttgaatcta tattaggaca taacaattct taaatcattg
     1921 ttagaaatta gtttgaattt tccaatcaat ttgttcgatt tttacatcat tgcattatat
     1981 catgtgacta ttaaccttgt tttctcctaa aattgatttt cttcttttct tatcttgtcc
     2041 actcgaaaca agtattgtaa gaatttgatt atttttgaaa gtacttttaa tatatttgag
     2101 atagttaaaa aagatttgaa actaaattcc aaattagaaa aagacttgaa atactaaaaa
     2161 aaaaaagtat actctaattg aaaacggtaa caaaactaat ttagagaatg aaatatagag
     2221 tatttctctc ttaaaagttt ttggtgaaac gagatgtaga aaggagattt agccaaagag
     2281 tctattagtg ctagaataat agattagaat tatagtgtat tgaatctata acagtacgca
     2341 ttaaatgcta aaatatttct ataaattaat ttaactttcc tgatagggct gttcatatct
     2401 tatttcaatt cactatataa ataaaattgt agtagatgtt attgaattag taaaaaaaat
     2461 aaaaaaacgg aggttttctg aatggaaact aaaaaaatta aaaatttaaa aggtcaaata
     2521 attgtttcct gccaagcgtt agaaggagag ccactttata ctccaaatgg aggagtgatg
     2581 ccgttattag caaaagccgc ctttcaagca ggcgctaaag gtattcgtgc taattctgtc
     2641 cgcgatattt cagaaataaa agaagaagtt gacttaccaa ttataggtat cataaaaaga
     2701 gattacgatg gctttgaacc ttttatttca gcaactatga aagagattga tgaattagtt
     2761 agtgaaggag tagacatttt agctttggat tgtactaatc gttcaagacc aggttatgat
     2821 aatattactg actttattca tgatattaaa gtcaagtatc caaaccaatt attaatggct
     2881 gatatttcta cttttgaaga agggaaagta gctgctgaat cgggagtaga ttttgtgggt
     2941 acgacattat ctggttatac accatatagc cctaaaaaag ataatcctga ttttgagtta
     3001 gttgaaagat tggtaaaaga gcttgacgta ccagttattg ctgaaggaag aataagtact
     3061 ccggaacaag ctagaaaaat gttagattta ggtgcttacg cagtggttgt tggaggagct
     3121 atcactcgtc ctttggaaat agcgaagaag tttattgagg tagtttagat gaatcaagaa
     3181 atattaaaaa agagtaagat tcaattgaca gttgaacata ttcaaaaata ttggatttta
     3241 tatttaatga tgattccagc aactgttcta ctaattttat ttacgtatgg accaatgtac
     3301 ggcattatta tggcctttca agactttact gtcttcaagg gatatactgg aagtccattt
     3361 gtcggtttaa aacattttca aagattgttc tcagatcctt tgttttatag attgttcaag
     3421 aatacgttca tggtaggggt tttagacttt ttattctctt tcccagctcc gcttattttt
     3481 gcactcatat taaacgaagt cagaaaagta agatttaaga gtgtagttca atctattagt
     3541 tatcttcctc attttattcc cctagtggtt atggttggta ttatttttga actatttggt
     3601 tcatatggta ttattaacag tttactttca tcacttggaa tggagccaat aagtttcttt
     3661 acaaaatctg aatggttctt gcctctctac attggttcag gagtatggaa aacaataggt
     3721 tggggttcta tcatttatat gggagcatta accaatatag attcaacttt atatgaagca
     3781 gcagatatgg acggggctaa tagatggcac aaaatgtggc atgtaactct accaagttta
     3841 agacctacag tagttacttt gtttatacta aatgctggtg gtatcatgca agtaggattt
     3901 gaaaaggtat tcttgatgtc tagtcctgca acatatgaag tatctgatgt attatctact
     3961 tatgtttata gacaaggtat tttaaattct gatttcagtt actcagctgc agtaggatta
     4021 tttaataaca tagtagcttt gttatttgtt ttattagcta ataagattgc taaaaaactt
     4081 ggcgaagaag gaattgtata aggggggtaa aatgattttt tcaaaaaata caaaaaagag
     4141 taatagagac aaagaaacct tcgttgatta tctctttata ttcattttaa taataataac
     4201 cttacttatg ttgtatccat tatggtgggt agtgatgacg tcactttcag aaccagtttg
     4261 gctatctaaa aacgttgtta aattatttcc cttaaaaata gatattactg catataagtt
     4321 tatttttggg gattctaaac tatggcaagc atatggtaat agtgtattgt atgctgcact
     4381 atctacaatt gttagtgttg cggtatgtag tatgtatgcc tatccactta caattcctga
     4441 aattaggggt aaaaaattct ggaatactct tattttgatt ccaatgttct ttagtggagg
     4501 aatgatcccg ctttatatac aagttgcaaa cctcggttta ttgaatactg cttgggctct
     4561 tgttttacct ggtgctgttt ctacatatta tataattttg tttagaacac agtttaaatc
     4621 aatcccaatg gagcttcgtg agtctgctag aattgatggg gcaagtgaat ttagaatttt
     4681 ttggaattta gtattaccat tatcaaaagt tattttcatg attatagcac tttattgcat
     4741 agttggttca tggaatagtt ttactcctcc attactttat ttgaatgacg acactaagat
     4801 gcctttatct atttatttga gaaatttagt tgttacaaat acaactgata ctgctgaatt
     4861 atcactttct gcaggtaata catattacca acaaattgct gctcaaggtg gaggtattgg
     4921 tatgcgtatt gctattaaga tgggtactat tgttgttagt gttattccaa ttatgatgat
     4981 ttatccattt attcagaaac attttgttaa gggaatcctg ctaggttctg tgaaaggtta
     5041 ataaggtaat tattttgcaa aatttaaaaa aatatctttt attagatagc ggtttagtag
     5101 ttgggtatac attagaaaat gctcaattaa aattaaataa gctaaaaaaa gatactgaaa
     5161 acaatccact atttggtgaa gagtattttg ccgaaaatcc taaattatgg gaagttcgtt
     5221 ttgataattc atatccaaac gttatctatg ataaagaatt gaaattatat agatgttggt
     5281 atcaaacatt tgttagtgat gaagcaagcg aagagactcc tttagcagaa cgtggagaga
     5341 aggaatacat tgtcaagtca tctagaatga cagcactttg ctatgctgaa agtaaagatg
     5401 gagtaaaatg ggaaaaacca aatctcaatc tagtaaaatt taaaggttca aaagataata
     5461 atattgttct gatgcatgca cacggtgcag gtatcatgct agatgaacag gagttggatc
     5521 ctaagaaacg atataaatta gtttcaaaat tagaattttc tccgaatctt cattacatgg
     5581 ttgttgtttt ttcagaagac ggtattcatt ttgacgaacc tattccatgg aaaaaatgga
     5641 atcctgcagc agatagtcat aattttccat ttagagatcc aaaaactggt aaatacgctg
     5701 ttattacaag aacctgggca aataacgtac gtctaagtgc aattactttc agtgatgatt
     5761 ttattaattg gtcagaacct aaagaaatcc ttaggggaga tggatttgaa gatcaaattt
     5821 attctatgcc agtaatttca tacgcggata tctatttagg ttttgcttca atgtaccacg
     5881 aaggcgacac tttaaatgaa aactatgata aagtagactt agaattgaaa ttctctgcta
     5941 atttggaaca atgggatagt gtatgtaagg gcgactatct cattgaaagg ggaggtggag
     6001 tatacccgca gaatgctgat tttgatgcta gttgtatttt cacggctgct ccaataattg
     6061 aagatgataa gatttggttc tactatatgg gtggaaatgg taatcacact ggcttccgag
     6121 aaagttcatt agctagagga tatattaaga aagaccaatt tgcttattat actaataaaa
     6181 gagaaggaat tccagctaaa atatctacag ttagctttca aacttacgat gatgaaattt
     6241 atatcttagc tgatattcct gaaaatggtt atgttaaagt tgcggtaggt actaaatacg
     6301 gtaatgctta ttcagatttt ggtcaggaag atatctctct tgttcaagtt tccgatggaa
     6361 agtataagtt ggaatttaat aaaaaatcta tcttagattt aggtaattct ccagttgcgt
     6421 tcaaatttga aatcgagaat gcaaaattgt atggtattga aggtagtatt cagcaacact
     6481 atctcaaata ctaaccagat taattcgtta acaaataatc tctatattag tggagataga
     6541 tatataaaat ggaggtctta ttatggtaaa gcttaataag gtattaaaag ggagtgtagc
     6601 actcctgagt gcaagtttat tgctagcagc ttgtggcaat aacgctaatt caaagcctgc
     6661 tgaaaaaaca gctgatggta aaacgaaaat ttcgtttcta tctacatggc aaattaatga
     6721 tgttcgtcca cctaaagatc aaacacttgt tggtcagtgg ttagaagaac agctaggtat
     6781 tgatattgat cttgtaacta ttcctggcga gggagtaagt tctaaagtaa attctcttat
     6841 tactagtaac cagttaccga ctgttttgtt gacaacagga gataagagtg atatcgctgg
     6901 tatcaacaaa cttggtaaac agggtgcatt cttagatttg tcaaaacaca tggacaaact
     6961 accaaactat aagaagtacc ttgaaaaaaa taatgcatta gctcaaatag aagatgatga
     7021 aaaacatatt tatgctttca caaaattctt tactgatgag aatatcatgt atactacacc
     7081 aattctgaga aaagacttac ttgttggttc agaatttgaa gatctttcaa aaattaaaac
     7141 tgttgatgac tatacaaaag ttttgaaata tttgactgaa aaacaaggtt ctccagcatt
     7201 tattcaacgt aatggctatg aaggcttcat gaagcgagtt acaccattgt ggaatttaag
     7261 ccatcgtaca tactatgact atgaatcaga ttcatataaa catccagttg aacaacctca
     7321 attgaaacaa ttcgttgaat ggttgaaaga attacgtaaa gataacgtac ttcaccctga
     7381 ctgggcagtt atgaaagatg aaacttggga aggtctattg gcttctaaca agggttcatt
     7441 cactgttgac cgtatgaata tcattggaga taataacttt gataaatcat ttgattggca
     7501 gccacttgtt tatccagaaa ttaatggaaa acgttatttg caaccgaaaa caccgtacat
     7561 ttctgattca ggttgggtaa tcaatgccaa tgcaccaaaa gaagaaattg ataaagcgtt
     7621 agaattcttt aatttcttat acgctgatga aaatcaagaa aaacttgcta ttggatttga
     7681 aggtaaaaca tttacacgtg aaaaccctaa tactcaagca ggtattaaat ggttgattca
     7741 aatttacggt gaaaatgctg gaaatgataa agcagaactg ctgtttaaac atggtaacca
     7801 agcatttact cgcgttcaaa ctaaattaga tattgaggca cagccaggaa cattccctaa
     7861 agttatgtat gatcaagtag ataaaattaa gaaagattta ggtggtttta gaccagatac
     7921 tccggttctt tcctttacaa ctgaagaatt agatgtttta actaaatctg aagcaggatt
     7981 aaatactttc tttgatgaaa atattattaa attcattgaa ggacaaagag atatgagtga
     8041 gtgggattca tttatcaatg aagctaaatc taaagacttg gataaagtta caaaagtata
     8101 taatgatgcc tacaaacgtg tcaaagatag aatgaaatag attaaatggt agctctctga
     8161 ttattcaggg agctactaag cctagaaagt gaatattcga aaatgtatga tgttgtagtt
     8221 ataggtgctg gtagttcagg tgctcttgca gcaattgctt ctggtcgcct aggattaaaa
     8281 acctgtttaa tcgaaaaagg gagtcgtata ggtggcgtac cgattaatac tctaatgggt
     8341 tcatttgcaa atctttactt cgatgattct gggagagtaa atgccagtaa gattgttggt
     8401 gagttaattg aacgtattat agaaaaaggt ggaactgttt tttcttcgat ggaagaattg
     8461 atgaatgtaa gcgactttta tcaaatcaca atcccgtacc aacctgaagt ctatgaatca
     8521 gttctaacag aaatgttaaa tgaatctaat gttgatattc ttctgaatgt agaaattaac
     8581 aatatagttt ttgacaacaa taagatacat agtgtagaaa tcatcagtaa tcaagaagta
     8641 aattatcttg aatcaaaagt ttttattgat gctacaggta atgctacgat agcttcagca
     8701 agtggcgcta atgtatatga aaaaaattct tcttatggct gtttaatgag agtgggtgga
     8761 gttgacatca ctaagactct tgaacatatt gaaaaaacga agcagtggga attcgaagaa
     8821 ggttataatg aatggctaga taatgtattg gctaaccgaa agtatagagc tacaggcgca
     8881 gtattgagag atcccgtttc ttatgatcat gctcctatgt taactaaaga tgaccatttt
     8941 atgaatgaag aaaaatggag ttatatcaag gaaaggtggg agagattcgg gtttatatat
     9001 actttagaag tatcattgtt tagaaatgaa ttaagagaag cagttgaagc tggtgatttt
     9061 gagtttgaaa tttatcatga tgatgaatct ggagtaacta tgaacggtga tggtatcagt
     9121 tatggttcat ggggtcaaaa tgtagcatta attaatgttg caaaagcatt tggattcaat
     9181 cccaaatata tggaagatga atcacatgct aatattcttt ctagaaaata taatttaaca
     9241 tttttatctt ttcttaaaaa atatgtacca ggttttgaaa attcttttat ccttgatcag
     9301 ggaactagag ctactaacag atcatgtagg cacattgata attcatctat gatggacgaa
     9361 attcttgatt ttccaatgta tacatttaag actatgtata gctatcaaac tcctaaagaa
     9421 ataacgtata aatcaattgt aggtggaaaa ttgaataatt tgtttgtaat aggtaaaggt
     9481 gcttaccaat ccgaaagttt tagatcacaa attagttgta tgttaatggg gatatcatca
     9541 gcagcagcgg caaaaactat tattgaagat aaaagcaata ctctaaacat taatagagat
     9601 ttgtttaaat cgaacttaga tatattattt acatgggagt tttctaattg gattactgat
     9661 taacaaataa taagggaata ttaaatgaat gatactaata aaaatcaata ctttgtttat
     9721 gaatttgata ctagctttgt agaaatgtct tattctcagt tttttgaagg atattgtatt
     9781 cttttttcaa aagaacccta tccttcttta tctgatatgc caatggaatt tagaaaagaa
     9841 tttttaatgg aaatgagtat attaggtgat gcgatgcttg aagtgttaaa tgcctataga
     9901 attaattatg atatcttagg aaattcaaca cctgtacttc atgctcattt atttccgaga
     9961 tacatgtggg aagaggaaga gagaagaaaa actgtagttc atagatatga ttcttcagtt
    10021 tttagcaatc ctgagtttga atttactata gaaaagcatg gaccaattat taaaaaactg
    10081 aggtctgcta ttgatagaca ttatagagag tatcaacaat tattagatga ttcaaactga
    10141 actccacatt ttcgattaga tttaatacct tgtatattac aactaggttg tggatgatat
    10201 aatttttcga aaaatatatt ttaggaatga cttataaagt aattatatca gaggttatta
    10261 aatcaatggt ccaagcttat gctccttatt cttattttaa ggttggagct gcgttagaaa
    10321 ctgaagataa aaattttttt ctggatgtaa tatagaaaat gttgcttttc tcctacttgc
    10381 tgtgcagaac gagtatattt ttacaaagct gtatttaaag gagaacgttc gttttcaaaa
    10441 atatgtattg taggcggaaa aaaaggtatt ttatcatctt ttatagcatc ttgcagggtg
    10501 tcttgtcagg taatgataga attttgtaat cttgattcat ttgtagttat attggctagt
    10561 gatgttgata cttataaagt atataaactt aaagaactac taccagttag ttttaatcaa
    10621 gaaaatttat aacaggaggg aactatggaa ttagttgaat ttaaaaatgg acatgttaat
    10681 acggttcatg agcatatcgc aataacgaaa gtaaaagagt ttctaaataa tcatgatata
    10741 ccaaaagata aaggtggcat acataaaatt tctactgatt tcttttacaa tataattgat
    10801 atggaaacta caactgaaga aaatagaccg tgggaaagtc ataaggaata ttatgatgtc
    10861 cattatctgt taaatggaga agaaataatt ttatataatt tcttgtctca aatggaattg
    10921 tctgaatata aagtagatga tgattggcaa caaatgaatg ggacagcttt atttagtatt
    10981 aaattaaaga aagatatgtt acttttgttg gaaccaaacg atgcacataa aacaggccta
    11041 ttggttgaag aaccaattaa tattaagaaa gttgttttta aggtaaaaat ttaactgaaa
    11101 gtactttcag ggattaagaa ttttaaaata tttaatactc atctgttttc aaaaagcatt
    11161 ctagtccatc tccgattaac gatggacttt atcacctctt tctccagtcc ttgtatgaca
    11221 tcttgaagtt gatttatgac atcttccaat atgggcatca acagctccat aacaatagcg
    11281 aaattctcgt atatagtgac tatggacatg tggacctact cctattggag cccaacaaga
    11341 tcccagttta ctgattctac tgaaaccagc ctcatcttgg tacatcaagc gaaccttacg
    11401 aaaacgtcta ctgattttaa agcgctttct tgtcttcttg aattgagatt ttatttttag
    11461 acgcgacaat ggtttgagcg tctgctttct taggatgttc tggacgtggc ataatatttc
    11521 gccagccatg gcgcttcaac agttgataga aggcatcacg tgtgtaggaa cgacctaact
    11581 cctttttata agcctgaaat aaggcatcaa ttgtaacaaa ttctcctgac tctgcagcct
    11641 tcaaatgacg ggcaagaaag gctttctctt cctcaactgt catatatgca tggttacgac
    11701 cactacgtgt ttcttgaagg agagagtcga gtccgaactc ctcatatttt tttacatttc
    11761 gctaaatcgt tgtttgatta cagtctaaaa gctctataat ctctttataa gatttgccca
    11821 tcagacgaaa tatagtagat tgaaactaga atagtacacc cccacttcta aaacattgtt
    11881 agaaatcgat ttgactgtcc tgatcgattt gtcctgttct tgtttcattt tactatagaa
    11941 tgatttgaag gcgtttataa tatttagctg tacgagagtc ttttaaaagt gttttgatgg
    12001 tttggatttc ttctttagtt gatttcatat cactattata taatgctttt tgattttagt
    12061 ctagtataaa tagcgagaaa tatctatcct atcttctaga atgtcttcca aacgaggaaa
    12121 ctctcgtaaa caaagaggtt ttagaggcct atttaccatg gactaaagtt gtacaagaaa
    12181 agtgcaaata agaaatctcc agattaggaa ctatccgtga gttctctagt ctggagattt
    12241 ttcaatagac ttcctgcgaa acaaaatatg gtataatagt tctatgaatt atgaagcaag
    12301 taaacaacta accgatgcac gatttaagcg tcttgttggt gttcaacgca cgatttttga
    12361 agagatgtta gctgtattaa aaacagctta tcaacttaaa cacgcaaaag gtggacgaaa
    12421 acctaaatta agcctagaag actttcttat ggccactctt caatatgtgc gagaataccg
    12481 cacttatgaa caaattgcgg ctgattttgg tattcacgaa agcaacttaa tccgtcggag
    12541 ctaatgggtt gaagtaactc ttgttcaaag tggttttacg atttcaagaa ctcctctcag
    12601 ttctgaggac acggtaatga ttgatgcgac ggaagtaaaa atcaatcgcc ctaaaaaaac
    12661 aattagcgaa ttattctggt aaaaa
//
LOCUS       DAVDWT010000048        12039 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVDWT010000048 DAVDWT010000000
VERSION     DAVDWT010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12039
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.048"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(141..213)
                     /locus_tag="VUW50_001864"
     tRNA            complement(141..213)
                     /locus_tag="VUW50_001864"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(178..180),aa:Thr,seq:ggt)
     gene            complement(313..822)
                     /locus_tag="VUW50_001865"
     CDS             complement(313..822)
                     /locus_tag="VUW50_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HET7983702.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(820..916)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            945..1376
                     /locus_tag="VUW50_001866"
     CDS             945..1376
                     /locus_tag="VUW50_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET7983703.1"
                     /translation="MKKPIQKFFNNEILAYLFFGIVTTLVAILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLLFTFLFVTQFP
                     HIIGQFVNENIDKINAIETIIAQILIIIINYILSKIYIFRK"
     gene            1458..1835
                     /gene="mscL"
                     /locus_tag="VUW50_001867"
     CDS             1458..1835
                     /gene="mscL"
                     /locus_tag="VUW50_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET7983704.1"
                     /translation="MLKDLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKEE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            1951..3241
                     /locus_tag="VUW50_001868"
                     /pseudo
     CDS             1951..3241
                     /locus_tag="VUW50_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937360.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(3396..4490)
                     /gene="hemH"
                     /locus_tag="VUW50_001869"
     CDS             complement(3396..4490)
                     /gene="hemH"
                     /locus_tag="VUW50_001869"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET7983705.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            4645..5868
                     /gene="pepT"
                     /locus_tag="VUW50_001870"
     CDS             4645..5868
                     /gene="pepT"
                     /locus_tag="VUW50_001870"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HET7983706.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(5904..6533)
                     /locus_tag="VUW50_001871"
     CDS             complement(5904..6533)
                     /locus_tag="VUW50_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983707.1"
                     /translation="MNNANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANGDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            6498..6719
                     /locus_tag="VUW50_001872"
     CDS             6498..6719
                     /locus_tag="VUW50_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983708.1"
                     /translation="MDLFQELICIVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(6828..7397)
                     /locus_tag="VUW50_001873"
     CDS             complement(6828..7397)
                     /locus_tag="VUW50_001873"
                     /inference="COORDINATES: protein motif:HMM:TIGR01363.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET7983709.1"
                     /translation="MLEITLKSPYQFAHILFQPTIVPHGGHYHFIPESDLSAGELAAT
                     YVFNPNDIVRDTGDAYIVRHGDHYHYIPKSSLNNPPSHSNTEEVGSSSSSVLSNTSLH
                     VHHEEEDGHGFDANRIISEDSEGFVIPHGDHNHYIKVQTKGYEAALKNKIPSLQSNYQ
                     PGTFDEKAVLAKVDQLLADSRSIYKDRLS"
     gene            complement(7441..8001)
                     /locus_tag="VUW50_001874"
                     /pseudo
     CDS             complement(7441..8001)
                     /locus_tag="VUW50_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(8227..11346)
                     /gene="phtE"
                     /locus_tag="VUW50_001875"
     CDS             complement(8227..11346)
                     /gene="phtE"
                     /locus_tag="VUW50_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HET7983710.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
     gene            complement(11556..>12039)
                     /locus_tag="VUW50_001876"
     CDS             complement(11556..>12039)
                     /locus_tag="VUW50_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET7983711.1"
                     /translation="VEHPNERPHSDNGFGNASDHVRKNKVDQDSKPDEDKEHDEVSEP
                     THPESDEKENHAGLNPSADNLYKPSTDTEETEEEAEDTTDEAEIPQVENSVINAKIAD
                     AEALLEKVTDPSIRQNAMETLTGLKSSLLLGTKDNNTISAEVDSLLALLKESQPAPIQ
                     "
BASE COUNT         3530 a         2334 c         2076 g         4099 t
ORIGIN      
        1 gcggctagtt tcctagttta ctctttgatt ttcattgagt attagtttta tttacacagg
       61 tgcataagaa atatattgga aaaatttggg gcagacataa caaaaaccct tgtaaatcaa
      121 gggcttttct gtctatttaa tgctaattgc cgggattgaa ccggcgacct catccttacc
      181 atggatgcgc tctgccaact gagctaaatc agcttacttg aaaagtatac tataatcaga
      241 aaagcttgtc aagtttcctt agacattttc cataagaaaa agccaggtaa gagtcaccca
      301 gcttgtcctg ttctatttac ttaaatcaat tcgacgacca attttaccaa taataatcgc
      361 tccaataatc aatgaagcaa attcaccgat tcctgttgta aaccaagtaa ggaagaatgg
      421 taattctgct acaatattta actctgcagc gatagtaaac atggaaattg aaaagaggat
      481 tgaaaagaag aaatgatctt ttcgaatcaa tccgttaaag aggtaatcct tgctatattt
      541 tgcaaaaagc caaacaccta aactgaggaa tactaaggtt gaaccaccac caacaaagac
      601 atctagcagt ccgaagctaa agaaattagc aatcatacaa ccgatggtaa ctccgatgat
      661 gtacttagga ttgtaaaaag ccataaagtt catcatttct gaaatacgaa actgataagc
      721 accatagctg atggcattta ggggcggtgt gacagtcaaa acgacgtaga tagcagcgac
      781 gattgcaata tctgcaacat cacgaatagt taattttttc atcttttctc ctttggcggt
      841 tgcccgcgtg taatatgctt ggtgaaagaa gctaagcacc aagggttgat tcattcaacc
      901 ttactagtat agcacaatag cctgctttat gctatactta aagcatgaaa aaacctattc
      961 aaaaattttt taacaatgaa atcttagcct atctcttttt tggtatagtg actactcttg
     1021 ttgctatact atcacgatta gtcatttatc agctaagtca tcaggaactc cttgctacgg
     1081 ccttggcaaa tattatcggt attttatttg cctttatcac aaatgatacg attgtattta
     1141 agcaaaaaaa gaaaaataga cttatccgtt tagtgaaatt ttctcttgct cgcctttcta
     1201 cttttctgtt agatttgctc tttacttttc tctttgtgac tcagtttcct cacatcatag
     1261 ggcaattcgt aaatgaaaac attgataaga taaatgctat tgaaacaata attgcacaaa
     1321 tactcattat tatcatcaat tatatcttga gcaagatcta tatttttaga aaataatgct
     1381 ccatttatga gccttttaat tgccttctat aatgatttag gatacaatga aacacgaaac
     1441 attgaaagga aaacagtatg ttaaaagatc ttaaatcatt cttgcttcga ggaaatgtta
     1501 ttgaccttgc tgtcggtgtt gtcattgcct ctgcttttgg tgctatcgtc acttcgcttg
     1561 taaacgacat tatcactcct cttattttaa atcccgcttt gaaagctgct aaagtcgaac
     1621 gtattgctca actttcttgg catggagtcg gctatggtaa ctttttaagt gctattatca
     1681 attttatctt tgtcggtacc gccctcttct ttattatcaa gggtattgaa aaagcacaga
     1741 aactgactgg cataaaggaa gaaaaaactg ccgaaaaaaa accaaccgaa ttggaagtcc
     1801 ttcaagaaat aaaagctctc cttgagaaaa aataatcgat taaaaggatt tagcgcaaag
     1861 ctcaatgtca ttaagttaag aaattcaaat acaattctgt atttgggttt cttttttatg
     1921 tgataaacta tagttaagga ggtatttccc atgataaaac agataaaagc ccacttgaat
     1981 aagagtattc agagtatcat tggccaaaaa gttgagttcg tcaaacaaga tgaacaggcc
     2041 tttactcgta aaagaaggtt atcactagaa actatgattc gtacaattct gggcatggga
     2101 ggaaaatcac tatcaaaaga attactagat gccagactga cagtttcaaa ttcagccctt
     2161 gttcaaagac gctatcagat aaaacctgaa gctttctatg ccctatttaa agaatttaca
     2221 gcacctattc cacttaatac tgattttcca atattcgctg cagatgggag tgatatctgt
     2281 attcctcgaa atcccatgga tacagaaacc tctatccaaa cccaaacgga tgttaaatcc
     2341 tataatctca tacacataaa tgccctatac gacttgacga ctggagtgta tagagatgtc
     2401 tctattcaag acaaacatgc acaacatgag cgcttagctc tgattcagat gatggaggct
     2461 tctccctttc gagaaagctc ttgttatcat ggatagaggc tatgaaagtt acaatctcat
     2521 ggctcatttt caagaaaaag gttggcttta tatcatccgt attcgagatg gaaaacaatc
     2581 catgccttcc tccttcaact tgccaaatac ggagtgtttt gatcaaaaag tttctctaaa
     2641 attatcacga aagcaaacca atcagctcaa aaaactttat cgtgattttc ccaatgacta
     2701 tcattttatt ccacacaatt ctatttttga ttttcttcca gaaacaagtc gaaaacaaga
     2761 tcctgtaaca ctctatgaac ttccttttcg tatggtgcgt ttaaaagtgg aagaaggaaa
     2821 atacgaaact ttagtgacga atacagacta ttcagtccaa gaattaaaaa atctctacgc
     2881 cagtagatgg ggcatagaga ctagttttcg tgacctgaaa tacagtattg gcttggtcaa
     2941 ttttcacgcc aaaaagaagg aagggattct ccaagaaatc tttgctcgct ttacaaattt
     3001 taacttttgt cgttgggtaa cctcgcaagt tgccatcgac agtagtcaca aaaaacaaag
     3061 atataaagtg tgtttctcag atgcggctta tgcctgccgt ttgtttttta acggttccct
     3121 ttcttcccac cagttgaaaa actacctcaa gaaacagtta tctattattc gaccgaatcg
     3181 aaaatattca agaaagataa aagctcaatc ggtagttgat ttcatctgta gagtaacata
     3241 aatctcaaaa aatagggtga aaaaaccttt atttgtcatg caaaaaatag aagatgtgat
     3301 agagaatcac tttgatgatc tcaatcgcac ctcctattca agtagttaga tcactaactt
     3361 aatgacattg agcgcaaagc taaatccttt tttctttact ctttgggtaa cttgcctgct
     3421 ttttcaagca tttttttgat aagataaggc atcttaacat tctcccgccc ttttttctca
     3481 atcagttttt tcacaaattc tggcatttgt aaatcttcag attcagctaa aatgtttttc
     3541 gtctcgtctg aaggaactaa ctcatcaaag gtctcttctt ctgggagaaa ttccttaaac
     3601 tcttgatttt cattgactct aactgtattg accaaagcat caatcaaacg agaatctgta
     3661 tttggcattg gtggacgatg gtagtttacc ccaaattcct ggcataagtc ataacattct
     3721 acatcattgt caaacaagac ctcaatatgc tcactgatga aactaatagg tacaaaaata
     3781 taatggtctg gatgttctgt ctgttctctg agatactcca aaacatctgg tttaatccat
     3841 ggaataccaa tatcactctc actctgccaa gtgttggtat attgttctga actcaagccc
     3901 agtttttcag cgactagctt gctattttcg aaaatttggt ctatataagg atcaccaaag
     3961 tccaaggcaa aaatgggcac actatgggct gaaaagatga ctttaaagct gtcttgcttt
     4021 acttcttctt ttaaaatctt agcaatttca tctgcccaat agtttaagag cgcttcttcc
     4081 tgataccagt ccttaatgac caaaaattga atttgtttgc tttctaaaaa tttttcatag
     4141 cccatgacag agtagaaaga ataatggggc tccaaaatca agcaaataca ctgttcaatt
     4201 ccgtctgctt ccatttgacc gatcacatct ggaataaagg gactggaaaa cttattagca
     4261 aagtagacac tgtattcatt ccccagccta gcttctacca aggcaacctc ttgacgggta
     4321 attttttgca gaggcgtgcc tccaattcgt acataattat catagagagt ctgaatctcg
     4381 tggtcttggg gtctcacccc acgacgaata tttgtgaaaa aatcagctac accttcaaag
     4441 gtaatctctt ctggtgaacc gaaggtcatc attaaaattg cttttttcat gactgcttcc
     4501 ttgtgatgtt tttatcaagt ttattttatc attaattcat cgataagtaa acgctaacat
     4561 agggacattc tcaatcttct gtcttttgtt tttctcttct ttctatgata caatggaaaa
     4621 aataaattca aaaggagttt ttttatgact tatccaaatc tcttggaccg cttcttaacc
     4681 tacgttaagg tcaacacgcg ctctgatgaa cactctacta ctactccaag tacacagagt
     4741 caggttgact tcgcaacaaa tgtcctaatt cctgaaatga aacgtgttgg actgcaaaat
     4801 gtttactatc taccgaatgg ttttgctatt ggaactttgc cagccaacga tccgtcttta
     4861 acacgtaaga ttggttttat atcgcacatg gatactgctg attttaatgc tgaaggagtc
     4921 aatccacagg taattgaaaa ctacgatggt ggtgtgattg aactagggaa ttctggtttc
     4981 aaactcgatc cagctgactt caagagtctt gaaaaatatc caggacaaac gctcatcaca
     5041 acagatggaa caagcttgct aggtgctgat gacaagtcag gaattgctga aattatgaca
     5101 gccattgaat atctaactgc tcatcctgaa attaagcact gtgagattcg tgttggtttt
     5161 ggtccagatg aagaaatcgg tgttggtgcc aataaatttg atgcagaaga ttttgatgtg
     5221 gattttgcct acactgttga tggtggtcca ctaggtgaac ttcagtacga gactttctca
     5281 gccgctggtg ctgaattgca tttccaaggc cgtaatgtcc accctggtac tgccaaaggg
     5341 cagatggtca atgcccttca gctagcaatt gattttcata atcaacttcc agaaaatgac
     5401 cgacctgagt taactgaagg ttaccaaggt ttttaccatc taatggatgt gacaggtagt
     5461 gttgaggagg cgcgtgcaag ctacatcatt cgtgattttg aaaaagatgc ctttgaagcg
     5521 cgtaaagcat ccatgcaatc tatcgctgat aagatgaatg aagaacttgg gagcgaccgt
     5581 gtcactctca acttgacaga ccagtactac aatatgaaag aagtcattga aaaagatatg
     5641 actccaatta ccattgctaa agccgttatg gaagatctag gtatcacgcc tattatcgaa
     5701 ccaatccggg gtggaacaga cggctctaag atttccttta tgggaatccc aactccgaat
     5761 atctttgcag gtggcgaaaa tatgcacgga cgttttgaat acgttagcct tcagactatg
     5821 gaacgtgcag ttgataccat cattggcatc gtagcttata aaggctaaaa agacgaggta
     5881 gctcagctac ttcgcctttc tttttattct actggttttt cttgatttcc agtagttgta
     5941 gaagattctg ttgtttcatt ttctgaagtt gattcagcag gtttagaatc tcttgtattg
     6001 cttggtttgt tttcgtcgct agcagtttca atgttagatt ctgcagttgc gtttggttgg
     6061 ttctcagcac tggtgttatc accatttgct tcagcatttc ttgctggact tgtttcttca
     6121 cttgcgctag cttttgactg gatttgatga ttcaaaacta gaatagcttt tgtcgattca
     6181 agtaaagctg ttttgtcttt actcttagca gaaagttgat ctaataatgc atccacctta
     6241 tcaaagtccg catcagatcc attattactt tctaaataag agtgaagcga catgagaata
     6301 tcgtagagtt tttgatagag tacaagtgtc tgaggatctt gctcagcatt ttccttttct
     6361 tgttgaaggg cgctagcgat acgagtcaag acatctttta cctgactgtt tacttcatcc
     6421 aagtctgcat cagccttgtt tgtggcagct tttagatttt ctacttcttc tgccaaggat
     6481 tgtctgattc cttcttcatg gatttgttcc aagagttgat ttgcattgtt caaaagactt
     6541 tctacttctt ccttgctatc tgtcgcagat tattggttgc tatctaccat gtactcctaa
     6601 aacaggagag ttataatcca agattacaag gccttacaga aataagaaat ccagataaga
     6661 caatgttcgt ccaagacgct attcgcttcg cacagcagca cggattcaat atgctttaat
     6721 tttaaagttt aggtgtcaag acctcttttt agtgtgccca aaatttagag aagtaatcaa
     6781 tcaactaact tttatttttt tcaaactttc agtaaactga cctaaagcta actcaatctg
     6841 tctttgtaga tgcttctgct atcagctaga agttgatcta cttttgccaa gactgccttc
     6901 tcatcaaaag ttccaggttg atagttggat tgcagggatg gaatcttgtt tttcaaagcc
     6961 gcttcatatc ccttagtttg aaccttgatg tagtgattgt ggtcgccatg aggaattaca
     7021 aaaccttctg aatcttcact tataattcga ttggcatcaa aaccatgacc atcttcttcc
     7081 tcatggtgga catgtagtga cgtattactt aatacagaac tagaagaact tcctacctct
     7141 tccgtgttag agtgtgatgg gggattgtta agagatgact taggaatata gtgatagtga
     7201 tccccatgtc ttactatata agcatcacct gtatctctga caatatcatt agggttaaag
     7261 acatatgtgg ctgctaattc acctgccgac aagtcactct caggaatgaa atgatagtga
     7321 ccaccatgtg gtactatagt aggttgaaat agaatatgag caaattgata aggggatttt
     7381 aaagtaattt ctaacaatga tttagaaact atgatgtgct attctaaatt caactcacta
     7441 tatataacca tcatcggtag tataacgtcc ctgtaatttt gctacagata cttctgcact
     7501 agctccttta tcgtctttac catgttcttg tttttggcga ttgatttcat cttttgttcg
     7561 tacattttct gcatgagctt gatctttaag gtaaacataa tactttccat ctaccttaat
     7621 aatatatcct cccttaacct aactgacgat atcttgatct ttcggctgat agttgggggc
     7681 tttcattaat agctcttcac taaagagcgc atcaaaagga actttaccat tatagtagtg
     7741 ataatgatcg ccatgagaag ttacataacc ttgacctgta atcttaataa caatttgttt
     7801 tgcttgaatt ccttcttttt gactaaccta gtctggagtc aaattttcag tcttcttagt
     7861 gtctttatta ctgtttacat atgaaacacg atttttatct gtattggcct gttagctatg
     7921 ttggttcaga gcataaacac acagacttaa ggaaaggata acaacagatc cagctgctat
     7981 atatttcttt ttaaatttca taattacctc atttctataa ttatttatat gatgtcttca
     8041 ttattaaatg attaaataaa ttaattaacc aattaattaa ctagtaaata ttccacctct
     8101 ttttaagttg tatgtcaaga aattttatat attaataata aaatgaaatt ctcccaaagt
     8161 cagagtttta tttctaactt ttgagagaac ttcatttttg attcagactt tttctactgc
     8221 tattccttac gctatgagat cagacaaatt cttttttatc acttctccac ttggcaatct
     8281 taattcaatc gttccatcca tattgaatat aacactatct aagcctaatc cgtaactagc
     8341 tgtaaatttt tctaattttt cttgtacagg atctactgct ggagcttcct ctaatgctgg
     8401 atctaacata gggtcactcc ccacattccc ttctggattc aacattccat tatccgttga
     8461 gttttctggt tttacaggtt tttcgtttgg tgcctctggt aaagaatctg ctggtttatt
     8521 ttctgttggt tggttctcaa ctgttccagt agatactttt ccattttcag atggtttatt
     8581 ttcaccattt ccttgaggtg cttctcctgt aaaatctgcc atattctttt taatgacttc
     8641 tcccgatggt aaatataatt caattgttcc gtccatatta aacaagacat tttctagctt
     8701 catcccataa ctttcagcaa attttgctac tttttcttgt acaggatcca ctgtaggaac
     8761 ttcttctaac gttgaattac tagtactatt cccagtttca gaaagttttt ctttttctac
     8821 cttctcacta gtctttggtt cttctacctt ttcatcaagt tttgagtttt cttgtgcttt
     8881 attcctttta aattgtggta gaatacttgg tttatcagtt tgattttctt tttccaagat
     8941 aggtacttcc acaatataag tcgattgatt gtccaaataa gcatttgcca tgaaggttac
     9001 aggaatttta tttccggccg ttctggttgt tccttggttt aatttcggaa tcggtaattt
     9061 gatttcacca actttatagt tattttctaa ataagcattt ccatgaaatt catcaaacac
     9121 tctgactaaa gcatcagttc ctttaggcac tgcaaattga gggttcactc ttaaataagt
     9181 atcccctgca tggaaaggat agaaaatcgt ttgactggcc attttgtaag ctaaagaggt
     9241 tggaactgta aatgtaccat cataacttac ttctggataa tcttttgaag cgatagtata
     9301 cttaaatgtt tgtcctggta aataaggttg atctaattca aagtttgcaa tattccctac
     9361 tccttctcca aatactttac cagatacttt ctccaatact tttccatctg gtgttattaa
     9421 ttttactagc atattgatac ctaatttttt ctccaattca ggcggaaaac taaaagaaac
     9481 gcgtttttga ccattggcta gagtaaagtt ttgattatta aacgtactat tttttaacaa
     9541 attaacaaca ttcgttaatt cttctccagt ataaacttta ttcccttctt ttttagcaac
     9601 tccttcttcg ggtttaaaca gttcatagtt actgtgagaa tgaccaattc caaccggttt
     9661 atgttcatca atcggatctg catgatggtg atctccatgc ggataaataa tcgcattttt
     9721 ttctttattc acgacaatac tttcacgttt gacaccatat tgtttcataa tgccagcaat
     9781 tttttcttcg atttttttat ctaaatcttt catttctttg gcattacttg gataatcctg
     9841 ttcatgagat gacaaagaat ctaatccatt atgactagtt ttaacttcct ctaaatgttt
     9901 ttgcgcagcc ttaatttgct cttctgtcaa gtccttcttg aagaaataat gattgtggtc
     9961 tccgtgactc atgacaaaac ctgattcatc ttcagcgata atacgattag catcaaatcc
    10021 gtatccatct tcttcatgtt tctcatgtga agttcctgga ttgattggaa gagatggaga
    10081 aggtgttgct agactattgt ttggaagagt cggttgccca atttgatttg attttggaat
    10141 gtaatggaaa tgatcaccat gtcttacaat ataagctgta gccgtttctt caacgatatc
    10201 ttttggatta aaaatataac catcagatgc tgaagagagc tccttacttg tcgttaaaga
    10261 agaaggattg cttgaaagac tgcctagact agacactact tcattaggtt ttgcatttgt
    10321 agaaactgta gaaccagttc cactgatagg caccattctg gcaatctttt cttctaaggc
    10381 agaaagcttg ctgtaaggaa taaagtggta atggtcgcca tgcggaatcg caactccatt
    10441 tggtgtacga ctgataatct tagcagggtc aaagaccagg ccatctgatt cactgtaacg
    10501 ttgggcgcta ggtgaatcat agagttcctt caaaagactc tggagatttt cagatttatt
    10561 tgctggcttg ctagttgatc cttttgctac agattgcgtg ttattgtcac tagctgttga
    10621 agaatagctt aactgactcg gttgcatatt ttttccagcc agatgtgctt tagctgctgc
    10681 taattcacta gcagataaat cgcttttggg aatgtagtga tagtgacctc catgaggaac
    10741 gatataagca ttacccgtat cttcgataat atcagctgga ttaaagacat aaccatcatt
    10801 tgtcgtatat cgtccctgag accttgctac agcaacatta gagttaacct tctcattatc
    10861 tttgacatgt tcttgttttt gacgattgat ttcatcttta gttcgaacat tatcagcatg
    10921 agctgcatct ttcaggtaga cataatattt tccatcgacc ttgatgatat aaccaccctt
    10981 gacttcattg acaatatcag cgtctttaag ttgatagttt ggatccttca tcaagagttc
    11041 ttcactaaag agggcatcat aaggaacttt cccattatag taatgatagt ggtcaccgtg
    11101 tgacgttaca tagccctgat ctgtaatttt gattacaatt tgctcagcct gaattccttc
    11161 tttctggcta acctggtctg gtgtcaagtt ttcacttttc tgacttgact ggctgccatc
    11221 cacataagag acacgattat tgtccttatt ttcctgcgaa cgatgctggt ttagtgcata
    11281 ggcacataga ctcaaggata cgataacagc tgatccagct gctatatatt ttttactaaa
    11341 tttcataaat ccctcatttc aataaatgat gaagtttttt ctcaacttct tttactttat
    11401 taaatagttt tctaaaccgg ttatttaacc atttaattaa ccagtaaata gtttactatt
    11461 taaaattact tttgtcaagt ttctgttacg tttttctaaa aaagagaagt aacacgaaag
    11521 gttacttctc cataaaatat gctccattca ttttactact gtataggagc cggttgactt
    11581 tcttttaaca aagccaagag actatctact tctgctgaaa tagtgttatt atctttcgtt
    11641 ccgagaagaa gactactttt tagaccagtc aatgtctcca tagcattttg tctaatacta
    11701 ggatctgtta ctttttctag caaggcctcc gcatctgcta tcttagcgtt aataacagaa
    11761 ttctctactt gaggaatttc agcctcatct gtggtatctt cagcttcttc ctctgtctct
    11821 tccgtatcag tgcttggttt ataaagatta tctgctgaag gatttaaacc agcgtgattc
    11881 tctttttcat cagattcagg gtgagttggc tcacttactt catcatgttc cttatcttca
    11941 tcaggtttac tgtcttggtc taccttattt ttacgaacat ggtcgctagc gttaccaaaa
    12001 ccattatctg aatgcggacg ttcgtttgga tgttcgaca
//
LOCUS       DAVDWT010000049        11166 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVDWT010000049 DAVDWT010000000
VERSION     DAVDWT010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11166
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.049"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            39..1154
                     /gene="ychF"
                     /locus_tag="VUW50_001877"
     CDS             39..1154
                     /gene="ychF"
                     /locus_tag="VUW50_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET7983712.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            1225..1794
                     /locus_tag="VUW50_001878"
     CDS             1225..1794
                     /locus_tag="VUW50_001878"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET7983713.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTHVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            1795..5304
                     /gene="mfd"
                     /locus_tag="VUW50_001879"
     CDS             1795..5304
                     /gene="mfd"
                     /locus_tag="VUW50_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET7983714.1"
                     /translation="MVTLLDLFSENDQIKKWHQSLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEMSQLEPCRIEFFGDEIDGIRSFEVETQLSKENKI
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELVLQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            5362..5628
                     /locus_tag="VUW50_001880"
     CDS             5362..5628
                     /locus_tag="VUW50_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET7983715.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            5621..5989
                     /locus_tag="VUW50_001881"
     CDS             5621..5989
                     /locus_tag="VUW50_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET7983716.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            6109..7377
                     /locus_tag="VUW50_001882"
     CDS             6109..7377
                     /locus_tag="VUW50_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HET7983717.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            7374..8651
                     /gene="tilS"
                     /locus_tag="VUW50_001883"
     CDS             7374..8651
                     /gene="tilS"
                     /locus_tag="VUW50_001883"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET7983718.1"
                     /translation="MREQDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNIDVENLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            8655..9197
                     /gene="hpt"
                     /locus_tag="VUW50_001884"
     CDS             8655..9197
                     /gene="hpt"
                     /locus_tag="VUW50_001884"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET7983719.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            9213..>11166
                     /gene="ftsH"
                     /locus_tag="VUW50_001885"
     CDS             9213..>11166
                     /gene="ftsH"
                     /locus_tag="VUW50_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET7983720.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQNPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDE"
BASE COUNT         3843 a         1651 c         2312 g         3360 t
ORIGIN      
        1 aatattaggg attgaaatga aaacggagaa tgagaaatat ggctttgaca gcaggtatcg
       61 ttggtttgcc aaacgttggt aaatcaacac tatttaatgc aattacaaaa gcaggagcag
      121 aggcagcaaa ctacccattt gcgactattg atccaaatgt tggaatggtg gaagttccag
      181 atgaacgcct acaaaaacta actgaaatga taactcctaa aaagacagtt cccacaacat
      241 ttgaatttac agatattgca gggattgtaa aaggagcttc aaaaggagaa gggctaggga
      301 ataaattctt ggccaatatt cgtgaagtag atgcgattgt tcacgtagtt cgtgcttttg
      361 atgatgaaaa tgtaatgcgc gagcaaggac gtgaagacgc ctttgtagat ccacttgcag
      421 atattgatac aattaatctg gaattgattc ttgctgactt agaatcagtg aacaaacgat
      481 atgcgcgtgt agaaaagatg gcacgtacgc aaaaagataa agaatcagta gcagaattca
      541 atgttcttca aaagattaaa ccagtcctag aagacgggaa atcagctcgt accattgaat
      601 ttacagatga ggaacaaaag gttgtcaaag gtcttttcct tttgacgact aaaccagttc
      661 tttatgtagc taatgtggac gaggatgtgg tttcagaacc tgactctatc gactatgtca
      721 aacaaattcg tgaatttgca gcgacagaaa atgctgaagt agtcgttatt tctgcgcgtg
      781 ctgaggaaga aatttctgaa ttggatgatg aagataaaaa agagtttctt gaagccattg
      841 gtttgacaga atcaggtgta gataagttga cgcgtgcagc ttaccacttg cttggattgg
      901 gaacttactt cacagctggt gaaaaagaag ttcgcgcttg gactttcaaa cgtggtatga
      961 aggctcctca agcagctggt attatccact cagactttga aaaaggcttt attcgtgcag
     1021 taaccatgtc atatgaagat ctagtgaaat acggatctga aaaggccgta aaagaagctg
     1081 gacgcttgcg tgaagaagga aaagaatata tcgttcaaga tggcgatatc atggaattcc
     1141 gctttaatgt ctaaaaatta ataaatggtg tcaattaggt tggaaaaaaa ttccaaccct
     1201 tttggctttt gaaaggaaaa ataaatgacc aaattacttg taggcttggg aaatccaggg
     1261 gataaatatt ttgaaacaaa acacaatgtt ggttttatgt tgattgatca actagcgaag
     1321 aaacagaatg tcacttttac acacgataag atatttcaag ctgacctagc atcctttttc
     1381 ctaaatggag aaaaaattta tctggttaaa ccaacgacct ttatgaatga aagtggaaaa
     1441 gcagttcatg ctttattaac ttactatggt ttggatattg acgatttact tatcatttac
     1501 gatgatcttg acatggaagt tgggaaaatt cgtttaagag caaaaggctc agcaggtggt
     1561 cataatggta tcaagtctat tattcaacat ataggaactc atgtctttaa ccgtgttaag
     1621 attggaattg gaagacctaa aaatggtatg tcagttgttc atcatgtttt gagtaagttt
     1681 gacagggatg attatatcgg tattttacag tctgttgaca aagttgacga ttctgtaaac
     1741 tactatttac aagagaaaaa ttttgagaaa acaatgcaga ggtataacgg ataaatggtg
     1801 accttattag atttattctc agaaaatgat cagattaaaa aatggcatca aagtttaaca
     1861 gataagaaaa gacaactaat acttggttta tcaacatcta ctaaggctct tgcaattgca
     1921 agcagtttag aaaaagaaga taggattgtt ttattgacgt caacttatgg agaagcagaa
     1981 ggacttgtta gtgatcttat ctctatcttg ggtgaggaac tcgtctatcc atttttggta
     2041 gatgatgctc ctatggtgga gtttttgatg tcttcacagg aaaaaattat ttcacgggtt
     2101 gaagccttgc gttttttgac tgattcatct aagaaaggga ttttagtttg taatatcgca
     2161 gcaagtcgat tgattttacc gtctcccaat gcattcaaag atagtattgt aaaaatctca
     2221 gttggtgaag aatatgatca acacgcgttt atccatcagt taaaggaaaa tggctatcga
     2281 aaagttactc aagtacaaac tcagggcgaa tttagtcttc gaggagatat tttagatatt
     2341 tttgaaatgt cccagttaga accttgtcga attgagtttt ttggtgatga aattgatggt
     2401 atcaggtcat ttgaagtaga aacacaatta tcgaaagaaa ataagataga actcactatc
     2461 tttccagcta gtgatatgct tttgagagaa aaggattatc aacgaggaca gtcagcttta
     2521 gaaaaacaaa tttcaaaaac tttatcacct attttgaaat catacctaga agaaattctt
     2581 tcaagttttc atcaaaagca aagtcatgca gattctcgga agtttttatc tttgtgctat
     2641 gataagacat ggactgtctt tgattatatt gaaaaagata ctccaatatt ctttgatgat
     2701 tatcaaaaat tgatgaatca gtatgaagtc tttgaaagag aattagcgca gtactttaca
     2761 gaagaattac agaatagtaa agcattttct gatatgcagt atttttctga tattgaacaa
     2821 atctataaaa aacaaagtcc agtgaccttt ttctctaatc ttcaaaaggg tttaggaaat
     2881 ctcaaatttg acaaaattta tcaattcaat caatatccta tgcaggaatt tttcaatcag
     2941 ttttcttttc taaaagaaga aattgaacga tataaaaaaa tggattacac cattattctg
     3001 cagtctagca attcaatggg aagtaaaaca ttggaggata tgttagagga atatcagatt
     3061 aaattggatt ctagagataa gacaagtatc tgtaaagaat ctgtaaactt aatagagggt
     3121 aatctcagac atggttttca ttttgtagat gaaaagattt tattgataac tgaacatgag
     3181 atttttcaaa agaaattaaa gcgtcgtttt cgaagacaac atgtttcaaa tgcagagaga
     3241 ttaaaagatt acaatgagct tgaaaaaggg gactatgttg tccatcatat ccatgggatt
     3301 ggtcaatatc taggaattga aaccattgaa atcaagggaa ttcatcgcga ttatgtcagt
     3361 gtccaatacc aaaatggtga tcaaatttct atccccgtgg aacagattca tctactgtcc
     3421 aaatatattt caagtgatgg taaagctcca aaactcaata aattaaatga cggtcatttt
     3481 aaaaaggcca agcaaaaggt taagaaccag gtagaggata tagctgatga tttaatcaaa
     3541 ctctactctg aacgtagtca gttgaagggt tttgctttct cagctgatga tgatgatcaa
     3601 gatgcctttg atgatgcttt cccttatgtt gaaacggatg atcaacttcg tagtattgag
     3661 gaaatcaaga gggatatgca ggcttctcag ccaatggacc gacttttagt tggggatgtt
     3721 ggttttggaa agactgaagt tgctatgcgt gcagccttta aagcagtcaa tgatcacaaa
     3781 caggttgtca ttctagttcc gacgacggtt ttagcgcaac agcactatac gaattttaag
     3841 gaacgattcc aaaattttgc agttaatgtt gatgtgttga gtcgctttag aagtaaaaaa
     3901 gagcagactg caacacttga aaaattgaaa aacggtcaag tcgatatttt gattggaaca
     3961 catcgtgttt tgtcaaaaga tgttgtgttt gctgatttgg gcttgatgat tattgatgag
     4021 gaacagcgat ttggtgtcaa gcataaggaa actttgaaag aactgaagaa acaagtggat
     4081 gtcctaacct tgaccgctac gccaatccct cgtaccctcc atatgtctat gctgggaatc
     4141 agagatttgt ctgttattga aactccgccg actaatcgct atcctgttca gacctatgtt
     4201 ttggaaaaga atgatagtgt cattcgtgat gctgtcttgc gtgaaatgga gcgtggaggt
     4261 caagtttact atctttacaa caaagttgac acaattgttc agaaggtttc agaattacag
     4321 gagttgattc cggaggcttc gattggatat gttcatggtc gaatgagtga agtccagttg
     4381 gaaaatactc tattagactt tattgaggga caatacgata tcttggtgac gactactatt
     4441 attgagacag gggtggacat tccaaatgct aatactttat ttattgaaaa tgcggaccat
     4501 atgggcttgt caaccttata tcagttaaga ggaagagtcg gtcgtagtaa tcgtattgct
     4561 tatgcttatc tcatgtatcg tccagaaaaa tcaatcagtg aagtctctga aaagagatta
     4621 gaagcgatta aaggatttac agaattgggc tctggcttta agattgcaat gcgagatctt
     4681 tcgattcgtg gagcaggaaa tcttttagga aaatcccagt ctggtttcat tgattctgtt
     4741 ggttttgaat tgtattcgca gttattagag gaagctattg ctaaacgaaa cggtaatgct
     4801 aacgctaaca caagaaccaa agggaatgct gagttggttt tgcaaattga tgcctatctt
     4861 cctgatactt atatttctga tcaacgacat aagattgaaa tttacaagaa aattcgtcaa
     4921 attgacaacc gtgtcaatta tgaagagtta caagaggagt tgatagaccg ttttggagaa
     4981 tacccagatg tagtagccta tcttttagag attggtttgg ttaaatcata cttggacaag
     5041 gtctttgttc aacgtgtgga aagaaaagat aataaaatta caattcaatt tgaaaaagtc
     5101 actcaacgac tgtttttagc tcaagattat tttaaagctt tatccgtaac gaacttaaaa
     5161 gcaggcatcg ctgagaataa gggattaatg gagcttgtat ttgatgtcca aaataagaaa
     5221 gattatgaaa ttttagaagg tctgctgatt tttggagaaa gtttattaga gataaaagag
     5281 tttaaggaag aaaattccat ttgatatttt tcttctataa aatagataaa aatggtacaa
     5341 taataaattg aggtaataag gatgagatta gataaatatt taaaagtatc gcgaattatc
     5401 aagcgtcgta cagtcgcaaa ggaagtagca gataaaggta gaatcaaggt taatggaatc
     5461 ttggccaaaa gttcaacgga cttgaaagtt aatgaccaag ttgaaattcg ctttggcaat
     5521 aagttgctgc ttgtaaaagt actagagatg aaagatagta caaaaaaaga agatgcagca
     5581 ggcatgtatg aaattatcag tgaaacacgg gtagaagaaa atgtctaaaa atattgtaca
     5641 attgaataat tcttttattc aaaatgaata ccaacgtcgt cgctacctga tgaaagaacg
     5701 acaaaaacgg aatcgtttta tggggggggt attgattttg attatgctat tatttatctt
     5761 gccaactttt aatttagcgc agagttatca gcaattactc caaagacgtc agcaattagc
     5821 agacttgcaa actcagtatc aaactttgag tgatgaaaag gataaggaaa cagcatttgc
     5881 taccaagttg aaagatgaag attatgctgc taaatataca cgagcgaagt actattattc
     5941 taagtcgagg gaaaaagttt atacgattcc tgacttgctt caaaggtgat aaaatggaaa
     6001 atttattaga cgtaatagag caatttttga gtttgtcaga tgaaaagctg gaagaattgg
     6061 ctgataaaaa tcaattattg cgtttacaag aagaaaagga aaggaagaat gcgtaaattc
     6121 ttaattattt tgttgctacc aagttttttg accatttcaa aagtcgttag cacagaaaaa
     6181 gaagtcgtct atacttcgaa agaaatttat tacctttcac aatctgactt tggtatttat
     6241 tttagagaaa aattaagttc tcccatggtt tatggagagg ttcctgttta tgcgaatgaa
     6301 gatttagtag tggaatctgg gaaattgact cccaaaacaa gttttcaaat aaccgagtgg
     6361 cgcttaaata aacaaggaat tccagtattt aagctatcaa atcatcaatt tatagctgcg
     6421 gacaaacgat ttttatatga tcaatcagag gtaactccaa caataaaaaa agtatggtta
     6481 gaatctgact ttaaactgta caatagtcct tatgatttaa aagaagtgaa atcatcctta
     6541 tcagcttatt cgcaagtatc aatcgataag accatgtttg tagaaggaag agaatttcta
     6601 catattgatc aggctggatg ggtagctaaa gaatcaactt ctgaagaaga taatcggatg
     6661 agtaaagttc aagaaatgtt atctgaaaaa tatcagaaag attctttctc tatttatgtt
     6721 aagcaactga ctactggaaa agaagctggt atcaatcaag atgaaaagat gtatgcagcc
     6781 agcgttttga aactctccta tctctattat acgcaagaaa aaataaatga gggtctttat
     6841 cagttagata cgactgtaaa atacgtatct gcagtcaatg attttccagg ttcttataaa
     6901 ccagagggaa gtggtagtct tcctaaaaaa gaagataata aagaatattc tttaaaggat
     6961 ttaattacga aagtatcaaa agaatctgat aatgtagctc ataatctatt gggatattac
     7021 atttcaaacc aatctgatgc cacattcaaa tccaaaatgt ctgccattat gggagatgat
     7081 tgggatccaa aagaaaaatt gatttcttct aagatggccg ggaagtttat ggaagctatt
     7141 tataatcaaa atggatttgt gctagagtct ttgactaaaa cagattttga tagtcagcga
     7201 attgccaaag gtgtttctgt taaagtagct cataaaattg gagatgcgga tgaatttaag
     7261 catgatacgg gtgttgtcta tgcagattct ccatttattc tttctatttt cactaagaat
     7321 tctgattatg atacgatttc taagatagcc aaggatgttt atgaggttct aaaatgaggg
     7381 aacaagattt tttaaatcat tttctcaaga agggatattt caaaaagcat gctaaggcgg
     7441 ttctagctct ttctggtgga ttagattcta tgtttctatt taaggtattg tctacttatc
     7501 aaaaagagtt agagattgaa ttgattctgg ctcatgtgaa tcataagcag agaattgaat
     7561 cagattggga agaaaaggaa ttaaggaagt tggctgctga agcagaactt cctatttata
     7621 tcagcaattt ttcaggagaa ttttcagaag cgcgtgcacg aaattttcgt tatgattttt
     7681 ttcaagaggt catgaaaaag acaggtgcga cagctttagt cactgcccac catgctgatg
     7741 atcaggtgga aacgattttg atgcgcttga ttcgaggaac tcgcttgcgc tatctatcag
     7801 gaattaagga gaagcaagta gtcggagaga tagaaatcat tcgtcccttc ttgcattttc
     7861 agaaaaaaga ctttccatca atttttcact ttgaagatac atcaaatcag gagaatcatt
     7921 attttcgaaa tcgtattcga aactcttact taccagaatt ggaaaaagaa aatcctcgat
     7981 ttagggatgc aatcttaggc atcggcaatg aaattttaga ttatgatttg gcaatagctg
     8041 aattatctaa caatattgat gtggaaaatt tacagcagtt attttcttac tctgagtcta
     8101 cacaaagagt tttacttcaa acttatctga atcgttttcc agatttgaat cttacaaaag
     8161 ctcagtttgc tgaagttcag cagattttaa aatctaaaag ccagtatcgt catccgatta
     8221 aaaatggcta tgaattgata aaagagtacc aacagtttca gatttgtaaa atcagtccgc
     8281 aggctgatga aaaggaagat gaacttgtgt tacactatca aaatcaggta gcttatcaag
     8341 gatatttatt ttcttttgga cttccattag aaggtgaatt aattcaacaa atacctgttt
     8401 cacgtgaaac atccatacac attcgtcatc gaaaaacagg agatgttttg attcaaaatg
     8461 ggcatagaaa aaaactcaga cgtttattta ttgatttgaa aatccctatg gaaaagagaa
     8521 actctgctct tattattgag caatttggtg aaattgtctc aattttggga attgcgacca
     8581 ataatttgag taaaaaaacg aaaaatgata taatgaacac tgtactttat atagaaaaaa
     8641 tagataggta aaaaatgtta gaaaacgata ttaaaaaagt cctcgtttca cacgatgaaa
     8701 ttacagaagc agctaaaaaa ctaggtgctc aattaactaa agactatgca ggaaaaaatc
     8761 caatcttagt tgggatttta aaaggatcta ttccttttat ggctgaattg gtcaaacata
     8821 ttgatacaca tattgaaatg gacttcatga tggtttctag ctaccatggt ggaacagcaa
     8881 gtagtggtgt tatcaatatt aaacaagatg tgactcaaga tatcaaagga agacatgttc
     8941 tatttgtaga agatatcatt gatacaggtc aaactttgaa gaatttgcga gatatgttta
     9001 aagaaagaga agcagcttct gttaaaattg caaccttgtt ggataaacca gaaggacgtg
     9061 ttgtagaaat tgaggcagac tatacctgct ttactatccc aaatgagttt gtagtaggtt
     9121 atggtttaga ctacaaagaa aattatcgta atcttcctta tattggagta ttgaaagagg
     9181 aagtgtattc aaattagaaa gaataatctt taatgaaaaa acaaaataat ggtttaatta
     9241 aaaatccttt tctatggtta ttatttatct ttttccttgt gacaggattc cagtatttct
     9301 attctgggaa taactcagga ggaagtcagc aaatcaacta tactgagttg gtacaagaaa
     9361 ttaccgatgg taatgtaaaa gaattaactt accaaccaaa tggtagtgtt atcgaagttt
     9421 ctggtgtcta taaaaatcct aaaacaagta aagaaggaac aggtattcag tttttcacgc
     9481 catctgttac taaggtagag aaatttacca gcactattct tcctgcagat actaccgtat
     9541 cagaattgca aaaacttgct actgaccata aagcagaagt aactgttaag catgaaagtt
     9601 caagtggtat atggattaat ctactcgtat ccattgtgcc atttggaatt ctattcttct
     9661 tcctattctc tatgatggga aatatgggag gaggcaatgg ccgtaatcca atgagttttg
     9721 gacgtagtaa ggctaaagca gcaaataaag aagatattaa agtaagattt tcagatgttg
     9781 ctggagctga ggaagaaaaa caagaactag ttgaagttgt tgagttctta aaagatccaa
     9841 aacgattcac aaaacttgga gcccgtattc cagcaggtgt tcttttggag ggacctccgg
     9901 ggacaggtaa gactttgctt gctaaggcag tcgctggaga agcaggtgtt ccattcttta
     9961 gtatctcagg ttctgacttt gtagaaatgt ttgtcggagt tggagctagt cgtgttcgct
    10021 ctctttttga ggatgccaaa aaagcagcac cagctatcat ctttatcgat gaaattgatg
    10081 ctgttggacg tcaacgtgga gttggtctcg gcggaggtaa tgacgaacgt gaacaaacct
    10141 tgaaccaact tttgattgag atggatggtt ttgagggaaa tgaagggatt atcgtcatcg
    10201 ctgcgacaaa ccgttcagat gtacttgatc ctgccctttt gcgtccagga cgttttgata
    10261 gaaaagtatt ggttggccgt cctgatgtta aaggtcgtga agcaatcttg aaagttcacg
    10321 ctaagaacaa gcctttagca gaagatgttg atttgaaatt agtggctcaa caaactccag
    10381 gctttgttgg tgctgattta gagaatgtct tgaatgaagc agctttagtt gctgctcgtc
    10441 gcaataaatc gataattgat gcttcagata ttgatgaagc agaagataga gttattgctg
    10501 gaccttctaa gaaagataag acagtttcac aaaaagaacg agaattggtt gcttaccatg
    10561 aggcaggaca taccattgtt ggtctagtct tgtcgaatgc tcgcgttgtc cataaggtta
    10621 caattgtacc acgcggccgt gcaggcggat acatgattgc acttcctaaa gaggatcaaa
    10681 tgcttctatc taaagaagat atgaaagagc aattggctgg cttaatgggt ggacgtgtag
    10741 ctgaagaaat tatctttaat gtccaaacta caggagcttc aaacgacttt gaacaagcga
    10801 cacaaatggc acgtgcaatg gttacagagt acggtatgag tgaaaaactt ggcccagtac
    10861 aatatgaagg aaaccatgct atgtttggtg cacagaatcc tcaaaaatca atttcagaac
    10921 aaacagctta tgaaattgat gaagaggttc gttcattatt aaatgaggca cgaaataaag
    10981 ctgctgaaat tattcagtca aatcgtgaaa ctcacaagtt aattgcagaa gcattattga
    11041 aatacgaaac attggatagt acacaaatta aagctcttta cgaaacagga aagatgcctg
    11101 aagcagtaga agaggaatct catgcactat cctatgatga agtaaagtca aaaatgaatg
    11161 acgaaa
//
LOCUS       DAVDWT010000050        10892 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVDWT010000050 DAVDWT010000000
VERSION     DAVDWT010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10892
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.050"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            95..1099
                     /locus_tag="VUW50_001886"
     CDS             95..1099
                     /locus_tag="VUW50_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7983721.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPEFVLKEAGQVIE
                     "
     gene            1168..2034
                     /locus_tag="VUW50_001887"
     CDS             1168..2034
                     /locus_tag="VUW50_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7983722.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGHMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            2031..2789
                     /locus_tag="VUW50_001888"
     CDS             2031..2789
                     /locus_tag="VUW50_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7983723.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFEQDFITILGGN
                     GAGKSTLFNTIAGTISLTSGTIRILGEDVTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLFPRRLASYKDKFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRVIQDLKQEEKAKMKISDYYQLFE"
     gene            2879..4639
                     /gene="dnaG"
                     /locus_tag="VUW50_001889"
     CDS             2879..4639
                     /gene="dnaG"
                     /locus_tag="VUW50_001889"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET7983724.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPYQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            4642..5751
                     /gene="rpoD"
                     /locus_tag="VUW50_001890"
     CDS             4642..5751
                     /gene="rpoD"
                     /locus_tag="VUW50_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET7983725.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            5765..6094
                     /locus_tag="VUW50_001891"
     CDS             5765..6094
                     /locus_tag="VUW50_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET7983726.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            6209..7252
                     /gene="cpoA"
                     /locus_tag="VUW50_001892"
     CDS             6209..7252
                     /gene="cpoA"
                     /locus_tag="VUW50_001892"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HET7983727.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            7268..8593
                     /locus_tag="VUW50_001893"
     CDS             7268..8593
                     /locus_tag="VUW50_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7983728.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLVAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            8624..8800
                     /locus_tag="VUW50_001894"
     CDS             8624..8800
                     /locus_tag="VUW50_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET7983729.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIVPVATDY"
     gene            8859..8993
                     /locus_tag="VUW50_001895"
     CDS             8859..8993
                     /locus_tag="VUW50_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HET7983730.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            9057..10367
                     /gene="obgE"
                     /locus_tag="VUW50_001896"
     CDS             9057..10367
                     /gene="obgE"
                     /locus_tag="VUW50_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET7983731.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            10377..10535
                     /locus_tag="VUW50_001897"
     CDS             10377..10535
                     /locus_tag="VUW50_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983732.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            complement(10587..>10892)
                     /locus_tag="VUW50_001898"
     CDS             complement(10587..>10892)
                     /locus_tag="VUW50_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7983733.1"
                     /translation="LLLFHFQNKEPEKLFGLIEDNLKQVHPIFQTVFKTFLKDKEKIV
                     NALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT         3312 a         1908 c         2537 g         3135 t
ORIGIN      
        1 atttttttga tggaggttag tatagaaaga aaacgatggc gtcgcttgtt tagataagtg
       61 aaatatgtta aaggaaataa aaaggagaaa cagaatgaaa aataaacgtt taattggaat
      121 tattgctgca ttagcagtct tagtagcagg aagcttgatt tattcttcaa tgaataaatc
      181 agaagctcag aataataagg atgagaagaa aataaccaag attggtgtgc ttcaatttgt
      241 gagccatcca tcccttgatt tgatttataa agggatccaa gatggacttg cagaagaagg
      301 atataaagat gatcaagtta aaattgattt tatgaactca gaaggtgacc aaagtaaggt
      361 tgcgacaatg agtaaacaat tggttgcaaa tgggaatgac cttgtggttg gtatcgcaac
      421 accagcagcc caagggttgg ctagtgcaac aaaagaccta ccggttatca tggccgctat
      481 tacagaccca attggtgcta acttggttaa agatttgaaa aaaccaggtg gcaacattac
      541 aggggtatct gaccacaatc cagctcaaca acaagttgaa ctcatcaagg ctctgacacc
      601 gaatgtgaaa acaatcggag ctctttactc aagtagcgaa gacaattcaa aaacacaggt
      661 cgaagaattt aaggcttatg ctgaaaaagc aggtctgaca gtggaaacat ttgcagttcc
      721 ttcaacaaat gaaattgcct caactgtcac tgttatgact agcaaggtag atgctatttg
      781 ggttccaatt gataacacca ttgcatcagg atttccaacg gttgtctcta gcaatcaaag
      841 ttctaagaaa ccaatttatc ccagtgcgac agctatggta gaagtaggtg gtttggcatc
      901 agttgtaatt gaccaacatg accttggtgt ggcaacaggt aaaatgattg tgcaagtctt
      961 gaaaggtgca aaaccagccg ataccccagt caatgtcttt tcaactggta agtcagtcat
     1021 caataaaaaa atagcacaag aactaggtat tactattcct gagtttgttc tcaaagaagc
     1081 aggacaagtc atcgaataaa taaggtaggg aggaggagac tcttcccatt ttttaaaaag
     1141 atgaatatag aaaggattaa ataaaatatg attgtttcca ttatttctca aggatttgtc
     1201 tgggctattc taggtctggg aatctttatg acatttagga ttttaaactt tccagatatg
     1261 acgacagaag gttccttccc tcttggggga gctgttgctg tcactttgat aaccaaaggc
     1321 gtgaacccat ttttagcgac acttgttgct gtaggagcag gttgtttggc tggaatggca
     1381 gcaggccttc tttatacaaa agggaagatc ccaaccttgc tctcagggat tttggtgatg
     1441 acttcttgtc actcaatcat gctcttgatt atgggacgtg caaatttagg cctgcttgga
     1501 accaagcaaa ttcaggatgt tttgcctttt gattcggatt tgaatcaact cttgacaggt
     1561 ctcatctttg tgagtattgt tattgctctc atgctctttt tcttggacac taaactcgga
     1621 caagcctata ttgctacagg ggataatcct gatatggcta gaagtttcgg gattcatact
     1681 ggacacatgg agctcatggg cttggtctta tcaaatggtg tgattgccct tgcaggtgcc
     1741 ctcattgctc agcaagaagg ttatgccgat gtgtctcgag ggatcggggt tatcgttgtg
     1801 gggcttgcaa gtttgattat tggagaagtt attttcaaga gtttgagctt ggcagagcgt
     1861 ttggttacta tcgttgtagg ttctatcgct tatcaatttt tagtgtgggc agttatcgca
     1921 cttggcttta atacaagtta ccttcgttta tacagtgcct tgattttagc agtctgcctc
     1981 atgattccaa catttaagca aacaatcttg aaaggagcca agttaagcaa atgacagcaa
     2041 ttgtagaatt aaaaaatgca accaaaatcg ttaaaaatgg ctttgatgaa gaaaagatta
     2101 ttttaaatga tgtttcctta gaaatttttg aacaggactt tatcacgatt ttgggtggaa
     2161 atggtgctgg taaatcaacc ctctttaaca ctattgcagg aaccatatca ctaaccagtg
     2221 gaactatccg tattttaggt gaggatgtta ccaagttttc accagagaag cgcgctaagt
     2281 atctgtctcg agtcttccaa gatccaaaga tggggacagc tcctcgtatg acagtggcag
     2341 agaatctcct gattgccaag tttcgtggtg aaaagcgtgg actatttccg agacgtctgg
     2401 ctagctataa ggataagttt caggcgacca ttgaaaaagt gggaaacggt cttgagaaac
     2461 acttgaatac accgattgag ttcttatctg gtggacaaag acaggctttg agtctcttga
     2521 tggcaacctt gaagcgacct gaattactct tgttagacga gcatactgct gccctggatc
     2581 caaagactag tgttgctttg atggaattga cagatgaatt tgttaagaaa gatcaattga
     2641 cagcccttat gattacccat catatggaag atgctctcaa atatggcaat cgcttgattg
     2701 tcatgaaaga aggacgggtt atccaagatt tgaaacaaga agaaaaagca aaaatgaaaa
     2761 tctctgatta ttatcaactc tttgaataaa gtagggaaaa tgagtgaaat ggtttacttt
     2821 ttttctgaaa taaagtatac tatataaagt aaactatgat aacatggagg tattgtgtat
     2881 ggttgacaaa caagtcattg aagaaatcaa aaacaatgcc aacattgtgg aagtcatagg
     2941 agatgtgatt tctttacaaa aggcaggacg gaactatcta gggctctgtc cttttcatgg
     3001 tgaaaaaaca ccttctttca acgttgtaga ggacaagcag ttttaccact gttttggttg
     3061 tggtcgctca ggtgatgtct ttaaattcat cgaggagtac caaggggtta cctttatgga
     3121 ggctgtccaa atcttaggtc agcgtgtcgg gattgaggtt gaaaaaccgc tttatagtga
     3181 acagaagcca gcctcgcctt accaagctct ttatgatatg cacgaagatg cggctaaatt
     3241 ttaccatgct attctcatga caacgactat gggcgaagag gccagaaatt acctttatca
     3301 gcggggtttg acagatgaag tgcttaaaca tttttggatt ggtttagcac ctccagaacg
     3361 aaactatctc tatcaacgtt tgtctgatca gtatcgtgaa gaggatttac tggattcagg
     3421 cctgttttat ctctcggatg ccaatcaatt tgtagacacc tttcacaatc gcattatgtt
     3481 tcccctgaca aatgaccaag gaaaggtcat tgccttctca ggtcgtatct ggcaaaaaac
     3541 ggattcacaa acttctaagt ataaaaacag ccgatcgact gcaattttta acaaaagtta
     3601 cgaattatat catatggata gggcaaaaaa atcttctgga aaaactagtg agatttacct
     3661 gatggaagga ttcatggatg ttattgcagc ctatcgggct ggaatcgaaa atgctgtggc
     3721 gtcgatggga acggccttga gtcgagagca tgttgagcat ctgaaaaggt taaccaagaa
     3781 attggttctt gtttacgatg gagataaggc tgggcaagcc gcgatattga aagcattgga
     3841 tgaaattggt gatatgcctg tgcaaatcgt tagcatgcct gataacttgg atcctgatga
     3901 gtatctacaa aaaaatggtc cagaagactt ggcctatcta ttaacaaaaa ctcgtattag
     3961 tccgattgag ttctacattc atcagtacaa acctgaaaac agtgaaaatc tgcaggctca
     4021 gattgagttt attgaaaaaa tagctccctt gattgttcaa gaaaagtcca tcgctgctca
     4081 aaacagctat attcatattt tagctgacag tctggcgtcc tttgattata cccagattga
     4141 gcagattgtt aatgagagtc gtcaggtgca aaggcagaat cgcatggaaa gaatttccag
     4201 accgacgcca atcaccatgc ctgtcaccaa gcagttatcg gctattatga gggcagaagc
     4261 ccatctactc tatcggatga tggaatcccc tcttgttttg aacgattacc gtttgcgaga
     4321 agactttgca tttgctacac ctgaatttca ggtcttatat gacttgcttg gccagtatgg
     4381 aaatcttcct ccagaagttt tagcagagca gacagaggaa gttgaaagag cttggtacca
     4441 agttttagct caggatttgc ctgctgagat atcgccgcag gaacttagtg aagtagagat
     4501 gactcgaaac aaggctctct tgaatcagga caatatgaga atcaaaaaga aggtgcagga
     4561 agctagccat gtaggagata cagatacagc cctagaagaa ttggaacgtt taatttccca
     4621 aaagagaaga atggagtaat aatggcaaca aaacaaaaag aagtaacaac atttgacgta
     4681 caggtagcag aatttatccg taatcataag caaaaaggga cagcaacaga tgatgaaatc
     4741 aatgcaagtc tggttattcc ttttaccttg gacgctgatg ggattgaaga tctcttgcaa
     4801 cggattcagg atgcaggcat ttctatcaca gataacgaag gaaatccaag tgcgcgtgtt
     4861 cttagcaatg aagaagaacc agaactcagc gatgaggact tgattgggtc aacttctgct
     4921 aaggtcaatg accctgtccg tatgtacttg aaagaaatag gggtcgttcc tctcttgacc
     4981 aatgaagagg agaaagagtt ggcactggct gttgaagctg gcgatatcga agccaaacaa
     5041 cgtcttgcgg aagccaatct tcgtttggtt gtttccattg ccaaacgcta tgtcggtcgt
     5101 ggcatgcagt tccttgactt gattcaagaa ggaaatatgg gcttgatgaa ggcggttgac
     5161 aagtttgact attctaaagg gttcaagttt tcaacttatg caacttggtg gattcgtcag
     5221 gctatcactc gtgctattgc ggaccaagct cgtaccatcc gtatcccagt tcacatggtt
     5281 gaaactatca ataaattggt tcgtgaacag cggaatctcc ttcaagaatt ggggcaagat
     5341 ccgacaccag aacagattgc tgaacgaatg gatatgacac ctgataaggt tcgtgaaatc
     5401 ttgaagattg cccaagaacc agtatctctt gaaactccta tcggtgaaga ggacgatagc
     5461 caccttggag actttatcga agatgaagtg attgaaaatc cagtggatta tacgactcgt
     5521 atcgtcttgc gtgagcaatt ggatgaaatc ttagatactc ttacagaccg tgaagaaaat
     5581 gttctgcgtc tacgttttgg actagatgat ggaaaaatgc gcacacttga agatgtgggg
     5641 aaagtcttta acgtaactcg tgagcgtatc cgtcagattg aagcaaaggc tttgagaaaa
     5701 ctacgccaac caagtcgcag caaaccgctt cgtgatttta ttgaagacta agagtgagga
     5761 aaatatggct tatacagaag agcaaattga aaacatcaaa acacggattt taacagcctt
     5821 ggaagaagtc atcgaccctg agttgggaat cgatattgtc aatcttggtt tgatctatga
     5881 gattcgtttt gacggtgaca cagggcaaac agagattgac atgactttga caactatggg
     5941 ttgtccctta gcagaccttt tgacagacca gatttatgat gctatgatag aggttccaga
     6001 agtaacggat actgaggtta aattagtctg gtatccagcc tggacagttg aaaaaatgag
     6061 tcgctatgcc cgcattgccc taggcattaa gtaaacatat aaaagcatgt gagagactgt
     6121 tggaaaagcg aggaaatttc ccctcttttc ctctagtctc tcctttcttt tgctgatttt
     6181 attcaaagaa aatgatataa tagtagttat ggagaaaaag aaattacgca tcaatatgtt
     6241 gagttcaagt gagaaagtag caggacaggg agtttcaggt gcttaccgtg aattagttcg
     6301 tcttcttcac cgtgctgcca aggaccaatt gattgttaca gaaaatcttc cgatcgaggc
     6361 agatgtgact cactttcata cgattgattt tccctattat ttatcaacct tccaaaagaa
     6421 acgctcaggg agaaagattg gctatgtgca tttcttgcca gctacacttg agggaagttt
     6481 gaaaattcca tttttcttaa agggaattgt gaaacgctat gtattttctt tttacaaccg
     6541 gatggagcac ttggttgtgg tcaatcctat gtttattgag gatttggtag cagctggtat
     6601 tccacgtgaa aaagtgacct atattcctaa ctttgtcaac aaggaaaaat ggcatcctct
     6661 accacaagaa gaggtagtca gactgcgcac agatcttggt cttagtgaca atcagtttat
     6721 cgtagtaggt gctgggcaag ttcagaaacg taaagggatt gatgacttta tccgtctggc
     6781 tgaggaattg cctcagatta cctttatctg ggctggtggc ttctcttttg gtggtatgac
     6841 agatggttat gaacactata agaaaattat ggaaaatccc cctaaaaatt tgatttttcc
     6901 aggcattgta tcgccagagc ggatgcgcga attgtatgct ctagcggatc ttttcttgtt
     6961 gcctagttac aatgagctct ttcctatgac tattttagaa gctgcgagtt gtgaggctcc
     7021 tattatgttg cgtgatttag atctctataa ggtgattttg gagggaaatt atcgggcgac
     7081 agcgggtaga gaagagatga aagaggctat tttggaatat caagcaaatc ctgctgcctt
     7141 aaaagatctc aaagaaaagg ctaagaatat ttccagagag tattctgaag agcatctgtt
     7201 acaaatctgg ttggactttt atgagaaaca agccgcttta gggagaaagt aaaaagtgag
     7261 gtaatctatg cgaattggtt tatttacaga tacctatttt cctcaggttt ctggtgttgc
     7321 gaccagtatt cgaaccttga aaacagaact tgaaaagcag ggacatgctg tttttatctt
     7381 tacgacgaca gataaggatg tcaatcgcta cgaagattgg caaattatcc gcattccaag
     7441 tgttcctttc tttgctttta aggatcgtcg ctttgcctac cgaggtttta gcaaggcact
     7501 tgaaattgct aaacagtatc agctagatat tatccatact cagacagaat tttctcttgg
     7561 cctgttgggg atttggattg cgcgtgaatt gaaaattcca gtcatccata cctatcacac
     7621 ccagtatgaa gactatgtcc attatattgc taaggggatg ttgatccgac cgagtatggt
     7681 caagtatctg gttagaggtt tcctgcatga tgtggatggg gttatttgcc ctagtgagat
     7741 tgtccgtgac ttgctatctg attataaggt caaggttgaa aaacgggtca ttcctactgg
     7801 gattgaatta gccaagtttg agcgtccgga aatcaagcag gaaaatttga aagaactgcg
     7861 tagtaaacta gggattcaag atggtgaaaa gacgttgctt agtctttcga gaatctccta
     7921 tgaaaaaaat attcaagcag ttttagtagc ctttgctgat gttctgaaag aggaagacaa
     7981 ggttaaactg gtagtagctg gggatggccc ttatctgaat gacctcaaag agcaagccca
     8041 gaacctagag attcaagact cagtcatctt tacagggatg attgctccta gtgagacggc
     8101 tctttactat aaagcggcgg attttttcat ttcggcatcg acaagcgaaa cgcaaggttt
     8161 gacctacttg gaaagcttag ccagtggaac acctgtcatt gctcacggaa atccttattt
     8221 gaacaacctc atcagtgata aaatgtttgg aaccttgtac tatggagaac atgatttggc
     8281 tggtgctatt ttggaagccc tgattgcaac accagacatg aacgagcata ccttatcaga
     8341 gaaattgtat gagatttcag ctgagaactt tgggaaacga gtgcatgagt tttatctgga
     8401 tgccattatt tcaaataact tccagaaaga tttggctaaa gatgatacgg tcagtcagcg
     8461 tatctttaag acagttttgt atcttcagca acaggtggtt gctgtacctg taaaaggatc
     8521 tagacgcatg ttgaaggctt caaaaacaca gttgatcagt atgagagact attggaaaga
     8581 ccatgaagaa tagaaagagg aacagctatg aaaaaaacaa ttaatgagaa gcggtcgtga
     8641 taaaaagatt gcgggtgttt gtgctggggt ggcccattat ctggatatgg atccgactat
     8701 cgttcaagtc atttggggtg ttcttacttg ctgttacgga gctggaattg tagcttacat
     8761 tattttatgg attatcgtac cagtagcaac tgactattga aaactagctc aattcatgct
     8821 aagataatag aaaatagaat gtaacgaagg agagaaaaat ggcatttgga gataatggaa
     8881 atcgtaaaaa aactatgttt gagaaaataa ccttgtttat cgtgattatc atgctagtag
     8941 caagtttatt gggaattttt gcaactgcaa ttggtgccct cagtaatcta taaaatagat
     9001 tcaagaaaat ttagtgactg ggatttccca gccctttttt aaagtgagaa gaaataatga
     9061 gtatgttttt agatacagct aagattaagg tcaaggctgg taatggtggc gatggtatgg
     9121 ttgcctttcg tcgtgaaaaa tatgtcccta atggaggccc ttggggtggt gatggtggtc
     9181 gtggaggcaa tgtggtcttc gttgtagacg aaggactacg taccttgatg gatttccgct
     9241 acaatcgtca tttcaaggct gattctggtg aaaaagggat gaccaaaggg atgcatggtc
     9301 gtggtgctga ggaccttaga gttcgagtac cacaaggtac gactgttcgt gatgcggaga
     9361 ctggcaaggt tttaacagat ttgattgaac atgggcaaga atttatcgtt gcccacggtg
     9421 gtcgtggtgg acgtggaaat attcgtttcg cgacaccaaa aaatcctgca ccggaaatct
     9481 ctgaaaatgg agaaccaggt caggaacgtg agttacaatt ggaactaaaa atcttggcag
     9541 atgtcggttt agtaggattc ccatctgtag ggaagtcaac acttttaagt gttattacct
     9601 cagctaagcc taaaattggt gcctaccact ttaccactat tgtaccaaat ttaggtatgg
     9661 ttcgcaccca atcaggtgaa tcctttgcag tagccgactt gccaggtttg attgaagggg
     9721 ctagtcaagg tgttggtttg ggaactcagt ttctccgtca catcgagcgt acacgtgtta
     9781 tccttcacat cattgatatg tcagctagcg aaggccgtga tccatatgag gattacctag
     9841 ctatcaataa agagctggag tcttacaatc ttcgcctcat ggagcgtcca cagattattg
     9901 tagctaataa gatggatatg cctgagagtc aggaaaatct tgaagaattt aagaaaaaat
     9961 tgtctgaaaa ttatgatgaa tttgaagagt taccagctat cttcccaatt tctggattga
    10021 ccaagcaagg tctggcaaca cttttagatg ctacagctga attgttagac aagacaccag
    10081 aatttttgct ctacgacgag tccgatatgg aagaagaagc ttactatgga tttgacgaag
    10141 aagaaaaagc ctttgaaatt agtcgtgatg acgatgcgac atgggtactt tctggtgaaa
    10201 aactcatgaa actctttaat atgaccaact ttgatcgtga tgaatctgtc atgaaatttg
    10261 cccgtcagct tcgtggtatg ggggttgatg aagcccttcg tgcgcgtgga gctaaagatg
    10321 gggatttggt ccgcattggt aaatttgagt ttgaatttgt agactaggag actggtatgg
    10381 gagataaacc gatatctttc cgagatgcgg atggtaattt tgtttccgcc gcagacgttt
    10441 ggaatgaaaa gaaattggaa gaactattta atcgtctcaa tccaaatcgt gccttgagat
    10501 tggcacgaac taaaaaggaa aatccatctc agtaaagaag ctaaaaaatc ccggctcttt
    10561 gtcaactgta gtgggttgaa gaaaagctaa gctcgagaaa ggacaaattt tgtcctttct
    10621 tttttgatat tcagagcgat gaaaatccgt tttttgaagt tttcaaagtt tcgaaaacca
    10681 aaggcattgc gcttgataag tttgatgaga ttattggtcg cttccagttt ggcattagaa
    10741 tagtgtagtt gaagggcgtt gacaatcttt tctttatctt tgaggaaggt tttaaagaca
    10801 gtctgaaaaa taggatgaac ctgcttaaga ttgtcctcaa taagtccgaa aagtttctct
    10861 ggttccttat tctggaagtg aaaaagcaag ag
//
LOCUS       DAVDWT010000051        10772 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVDWT010000051 DAVDWT010000000
VERSION     DAVDWT010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10772
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.051"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            196..351
                     /locus_tag="VUW50_001899"
                     /pseudo
     CDS             196..351
                     /locus_tag="VUW50_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            551..3826
                     /gene="rexB"
                     /locus_tag="VUW50_001900"
     CDS             551..3826
                     /gene="rexB"
                     /locus_tag="VUW50_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HET7983734.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTILDDIGLGLAFYKCLAELNPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMNFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLHPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            3823..7473
                     /gene="addA"
                     /locus_tag="VUW50_001901"
     CDS             3823..7473
                     /gene="addA"
                     /locus_tag="VUW50_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HET7983735.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKPHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASAAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSFQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7688..8866
                     /locus_tag="VUW50_001902"
     CDS             7688..8866
                     /locus_tag="VUW50_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983736.1"
                     /translation="MTLPVRKSLHNAVLQASEADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWILNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            9075..>10772
                     /locus_tag="VUW50_001903"
     CDS             9075..>10772
                     /locus_tag="VUW50_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET7983737.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPIPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVTDKGEPAVQPELPEAVVTDKD
                     EPEVQPESPDTVVSDKGEPEQVAPLPEYTGPQAGAIVEPAVQPALPEAVVSDKGEPEQ
                     VAPLPEYKGNIEQVKPETPVEKPKEQGPEKTEEVPVKPTEETPVNPNEGTTEGTSIQV
                     "
BASE COUNT         3453 a         2181 c         2262 g         2876 t
ORIGIN      
        1 tacgaaaatc tattgaatat gccataagaa gattatacct cattgtgtac tatttttggt
       61 tcattttact atagtagatt gaaataagat acgaacagct tgattaggga aatcaaatta
      121 atttctagaa atattttagc atccacggtg tactattata gtttcaattt actatagttc
      181 aatcattcta tcaagaatat tgaatatata aaggcacgta atattaaaac ttcttctgat
      241 atcgaattag agtccgatgt tgatggcgat aaatcagaca atctaccagt cgacattaag
      301 atactgggac aacacattga agtgtattcc gtacctaaga aacaatcata aaactcaaag
      361 tcttgttacc gaggcttttt tattatgtga ttttcagtag ccagacttat tctatctaat
      421 acaagtaaag ctaagtattt aagatttagc agatgatgaa gaaaagaaag gatggcctaa
      481 atattttaac agatttacga ggctaatcat ggtataatat agggtgatga aaaattccaa
      541 cgaggctgag atgaaattac tttatactga tattcggact tctttgacag aaattctaac
      601 aagagaggca gaagagctag ttgcagctgg caagcgggtc ttctacattg cccccaactc
      661 tctttctttt gaaaaggaac gcgccgtgct ggaatgcttg tcccagcagg cttctttttc
      721 gattaccgtc acgcgctttg ctcaaatggc tcgttatctg gtcttgaatg atttaccagc
      781 taaaactatt cttgatgata tcggtcttgg gttggccttt tacaaatgcc ttgccgaact
      841 caatcccaag gacttgcgtg tttatggcgc tattaagcag gatcctcaat tgatccagca
      901 gttaattgag ctttaccatg agatgaccaa atcccagatg aattttttgg acttggagaa
      961 tttaacagat gaggataaga gggcggattt actcttgatt tttgagaaag taacagccta
     1021 tcttaatcaa ggtcagttag cccagggaag tcagttgtcc catttgattg aggctattga
     1081 gaatgacaag gtaagtagtg attttaatca aatcgccttg gtcattgacg gctttactcg
     1141 tttttctgct gaggaagagc gggttgtgga cttacttcac ggcaaaggtg ttgagattgt
     1201 tatcggggct tatgctagta agaaagccta taccagtcct tttagcgagg gcaatctcta
     1261 ccaagccagc gtaaaatttc tccatcatct ggcttctaaa taccaaacgc ctgctcagga
     1321 ctgttctcaa actcatgaga agatggatag ttttgacaag gcctctcgtt tgttggagtc
     1381 ttcttatgac ttttcagaac tcgctttgga tgtcgatgag aaagaccgtg aaaatttaca
     1441 aatctggtct tgtttgacgc aaaaggagga gttggagcta gtagcccgta gtattcgtca
     1501 gaaattacat gagaactcag acctgagcta caagcatttt cgtattctct tgggggatgt
     1561 agcttcttac cagttatctc tcaaaaccat ttttgaccag tatcagattc ctttttatct
     1621 tggtagaagc gaagccatgg ctcatcatcc cttgactcag tttgtcgagt ctattttagc
     1681 tttaaaacgt taccgttttc gtcaggagga tttgatcaat cttcttagaa ctgacttgta
     1741 tactgacctc agtcagtctg atattgatgc ttttgagcaa tatatccgct atcttggtat
     1801 caatggcttg ccagcctttc agcaaacctt caccaaatcc caccatggaa aatttaatct
     1861 tgagcgtttg aatgtcctcc gcctgagaat tttagcacct cttgaaaccc tctttgccag
     1921 ccgaaaacaa aaggctgaaa acctcctaca aaaatggagt gtctttctaa aagaaggagc
     1981 tgtgaccaag cagttacaag atttgacaac cactttggaa gctgtagaac aggaaagaca
     2041 agccgaagtt tggaaggctt tctgccatgt tttagaacaa tttgcgactg tttttgctgg
     2101 ttcacaggtt agtctggaag acttcctagc cttgctccat tctggaatga gtttgtccca
     2161 ataccgtacc attccagcaa cagtggacac tgttctggtg cagagttacg atttgattgc
     2221 accattgact gctgactttg tctatgctat tggactaact caggacaatt taccaaaaat
     2281 ttctcaaaac accagtcttc tgacagatga agaaaggcaa aacctaaacc aagcgaccga
     2341 agaaggcgtt caattactga ttgccagcag tgaaaatctc aagaaaaatc gctacactat
     2401 gctttccttg gtcaattctg ctcgtaagca gttgttcttg tcggctccaa gcctttttaa
     2461 cgaaagtgaa agtaaggaat ctgcctatct tcaagagttg atccattttg gatttaggcg
     2521 gagagagaag aggatgaatc acaaaggact gtctaaggag gatatggggt cctatcacag
     2581 tcttttgtct agtctggttg cctatcacca gcagggtgag atgagcgata ctgagcaaga
     2641 tttgactttt gtcaaggttc tgtcgcgtgt cataggtaaa aaactagatc agcaaggtct
     2701 ggaaaatcca gctatcccaa ccagtccaag cagcaagacc ttagccaagg acaccttgca
     2761 agctctctat cctgccaaac aggagtttta cctgtctacg tcgggtttga cagagtttta
     2821 tcgcaatgaa tacagttatt tcctacgcta cgttttaggc ttgcaggagg aattacgttt
     2881 gcatcctgat gcccgtagtc acgggaattt cttgcatcgt atctttgaac gcgccttaca
     2941 gttgcctaat gaagattcct ttgaccaacg tctagaacaa gctattcaag aaaccagtca
     3001 agaacgcgaa tttgaagcta tttatcaaga aagtttggaa gcccagttta ccaaggaagt
     3061 tttgcttgat gttgcacgga cgactggaca tattctccga cacaatccag ccatcgaaac
     3121 catcaaagaa gaagcaaatt ttggtggaaa agaccaagcc tttattcaat tagacaatgg
     3181 acgcagtgtc tttgtacgag gcaaggtgga ccggattgac cgtttgaaag ctaatggagc
     3241 gataggagta gtagactaca aatccagtct gactcagttc cagtttcctc atttctttaa
     3301 tgggctcaat tctcagttac caacctatct tgctgcccta aaaagagaag gggagcagaa
     3361 ctttttcggc gccatgtact tggaaatggc tgaacctgtc caatctctga tggcggtaaa
     3421 aagtctggcg ggtgcagtag tagaagccag caagtcaatg aaataccaag ggctcttctt
     3481 ggaaaaagaa agcagttatt taggcgaatt ttataacaaa aacaaggcta atcaactgac
     3541 agatgaggaa tttcagctcc tactggacta caatgcctat ctttacaaga aagctgctga
     3601 gaagatttta gcaggccggt tcgccatcaa tccttatact gaaaatggca gaagcattgc
     3661 cccatacgtc cagcaacatc aggctattac aggctttgaa gccaattacc atctgggcca
     3721 agcccgtttc ctagaaaagt tggacctagc tgatggcaag cgtctggtcg gagaaaaact
     3781 caagcaagct tggcttgaaa aaataagaga ggagttgaat cgatgaagct tattcccttt
     3841 ttaagtgagg aggagattca aaaactgcaa gaagcagaag caaattcgag caaggaacag
     3901 aagaaaactg ccgagcaaat cgaagctatc tacacttctg gccagaatat cctggtctca
     3961 gcatcggctg gttctggaaa gacctttgtc atggcagagc gcattctgga ccaattggcg
     4021 cgtggtgtcg aaatttctca actctttatc tcaaccttta cggtcaaggc tgcaactgaa
     4081 cttaaagaac gtttagagaa aaaaatcagc aagaaaatcc aagaaacaga tgatgtcgac
     4141 ctcaaacaac acttgggacg ccagttggca gacctaccca acgctgccat cggaaccatg
     4201 gactctttca cacaaaaatt ccttggcaaa catggttatc tgcttgatat tgcacctaat
     4261 ttccgtattt tacaaaatca aagcgagcaa cttcttctcg aaaacgaagt ctttcatgag
     4321 gtctttgaag cccattacca aggtaaacag aaagagacct ttagtcattt gctgaaaaac
     4381 tttgctgggc gtggcaagga cgaacggggt ctgcgccagc aagtctataa aatttatgac
     4441 ttcctccaat ccaccagtaa tcctcaaaag tggctgagtg aatctttcct caaaggtttt
     4501 gagaaagctg attttaccag tgaaaaagaa aaactgactg agcaaatcaa acaagccctt
     4561 tgggatttgg aaagcttttt ccgttaccat ctggataacg atgccaagga gtttgcaaag
     4621 gctgcctatt tagaaaatgt tcagttaatt ctggatgaaa ttggctccct aaatcaggag
     4681 ttcgatagtc aggcttatca ggcagtactt gcgcgtgttg tcgccatctc taaggagaaa
     4741 aacggtcgtg ctctgactaa tgccagccgt aaggctgatt tgaagcccct ggctgatgcc
     4801 tacaacgaag agagaaagac ccagtttact aaactaggac aattatcaga ccagatagcg
     4861 attctcgact atcaagaacg ttatcatgga gacacttgga aactagctaa aaccttccaa
     4921 tctttcatga gcgattttgt agaggcttat cgtcagagaa aacgacagga aaatgccttt
     4981 gaattcgctg atatcagcca ttacaccatt gagattttag aaaatttccc acaagttcgt
     5041 gagtcttatc aggagcgctt ccatgaagtc atggtcgatg agtatcagga taccaaccat
     5101 attcaagaac ggatgctgga attgttgtct aatggccaca atcgctttat ggtgggagat
     5161 atcaagcaat ccatctatcg tttccgtcag gcagacccgc agattttcaa tgagaaattc
     5221 caacgctatg cgcaaaatcc tcaagaaggc aagctcattc tcctcaagga aaatttccgt
     5281 agtagttcag aagtgctgtc agcaaccaat gatgtctttg aacgtctcat ggaccaagag
     5341 gtcggcgaaa tcaactatga taacatgcac cagcttgttt ttgccaatac caaactgact
     5401 cccaatccag acaacaaggc tgaatttctc ctctacgaca aggacgatac aggtgaggaa
     5461 gaagagagtc aaacagaaac gaaactaaca ggcgaaatgc gcttagttat caaggagatt
     5521 ctgaaacttc atcaagaaaa aggtgttgcc tttaaggaaa ttgcccttct gacctccagc
     5581 cgcagtcgta atgaccagat tctcctcgcc ctgtctgagt acgggattcc tgtcaaaact
     5641 gacggagagc aaaacaatta tctccaatcc ctagaagtgc aagtcatgct agacactctt
     5701 cgtgtcattc acaatcccct gcaagactac gccttggttg cccttatgaa gtctccaatg
     5761 tttggttttg atgaggatga gctagcacgt ttgtcccttc agaaagcaga ggataaagtc
     5821 cacgaaaatc tctatgagaa actggtcaat gcacaaaaaa tggcaagtag tcaaaaaggc
     5881 ttgattcaca cagctctagc tgaaaaacta aagcaattca tggatatcct agcttcttgg
     5941 cgcttgtatg ccaaacccca ctctctctat gacttgattt ggaagattta caacgaccgt
     6001 ttttattatg actatgttgg ggctttgccg aatggtcctg ctaggcaggc caatctctat
     6061 gccctagcac tgcgtgctga tcaatttgaa aagagcaatt tcaaaggttt gtcgcgtttt
     6121 attcgtatga ttgaccaagt cttagaagcc cagcacgatt tggcaagcgc ggccgtcgca
     6181 ccgccaaaag atgcagtaga gctcatgacc atccacaaga gtaaagggct ggagtttcct
     6241 tacgtcttta tcctcaatat ggatcaagat ttcaacaagc aagactctat gtcagaagtc
     6301 attctcagtc gtcagaatgg tcttggtgtc aaatatattg ccaagatgga gacaggggca
     6361 gtagaagacc actatcctaa aaccatcaaa ctctccattc ctagtctgac ctataggcag
     6421 aacgaagagg aattacagct agcaagctat tctgagcaga tgcgtttgct gtatgttgct
     6481 atgacgcggg ctgagaaaaa gctctatctt gtcggcaagg gttctcgtga aaagctggaa
     6541 tccaaggaat acccagcagc caaaaatggg aaactaaata gcaatactag actgcaagca
     6601 cggaatttcc aagattggct ttgggctatc agtaaagtgt ttactaagga caagctcaac
     6661 tttagttatc gttttattgg cgaagatcag ttgaccagag aagctatcgg agagttggaa
     6721 accaagagtc ctctccaaga tagcttccaa gcagacaatc gtcagtcaga taccatcaaa
     6781 gaagctctgg aaatgctgaa ggaggtggaa gtttataata ctcttcaccg cgcagctatt
     6841 gaacttccta gtgttcaaac cccaagtcaa atcaagaaat tttacgaacc agttatggat
     6901 atggaaggtg tcgagattgc tggtcaaggt cagtcagtag gcaagaaaat cagcttcgat
     6961 ttgccagatt tttcaaccaa agaaaaggta actggagctg agattggtag tgctactcac
     7021 gaactcatgc agagaattga cctcagccag caactaaccc ttgctagcct aacagaaaca
     7081 ctcaaacaag ttcaaactag ccaagctgtc agagacaaga tcaatcttga taaaattctt
     7141 gctttctttg acacagcact cggtcaggaa attcttgcta ataccgacca tctctatcgc
     7201 gagcaacctt tctccatgct caaacgagac caaaagagtc aggaagactt tgttgtccgt
     7261 ggtatccttg atggctatct gctttacgaa aacaaaattg ttctgttcga ctacaagaca
     7321 gaccgctatg atgaaccaag tcaactcgta gaccgctatc gtggtcagtt agctctatac
     7381 gaagaggctt tatcacgagc ctattcgatt gaaaatattg aaaaatactt gattttactc
     7441 ggtaaagacg aggttcaagt tgtaaaagta taacttatac tcttcgaaaa tctcttcaaa
     7501 ccacgtcagc ttcgccttgc tgtatatatg gttactgact tcgtcagttc tatccacaac
     7561 ctcaaaacag tgttttgagc tgacttcgtc agttctatct acaacctcaa aacagtgttt
     7621 tgagctgact gcggctagct ttctagtttg ctctttgatt tttattgagt attagaaagg
     7681 agacctcatg acacttccag ttagaaaatc cctacacaat gcggttttac aggcttcaga
     7741 agcggatact tgggaacaag ctaccaagga atggaatgaa gtttccttga tttttaacgg
     7801 tatcggccgt agcaattgtg tctgtggaaa tgccatcaaa tacgcttatg aactctttaa
     7861 cggtgtcaca ggccaacgcc tctttccaat tggtagcgac tgtgttcgtc attttcatcg
     7921 attgtccctt gaccagcaat tggaagagga agaaaaactg ctcagaaagg ttgaaaatct
     7981 aaccagaaaa gctcagaaaa aggaaaaaat caaggtcaat aaaagtgact ttgacgagcg
     8041 acttctaaaa tggctctggg aaaaaggtgt tttcaaaccc aatcgtggca atcaatttgc
     8101 gcctgagaga gactaccagc ttttcctaga agtctttcag ggaggaagtt ggaccaaggc
     8161 ggaaccaaag aaaaaggctc ggatggaaga agtccttgaa aagtgtatca aacccttttt
     8221 acttgggaaa tcggatgacc aactctacct tgtcaagcta ggcaaggaga aaattgacta
     8281 cgaacaagaa ctccgtatcc aggcagagaa agaacgcaag aagagggata aaatcgccaa
     8341 gcaatatgca gacaatctgg ttctagccat gggacccgca gagcgtgctt atcaagatta
     8401 ctttggcttt acagagaccc tgactcaaga agaacgcaag tgggaaaaaa ttctttttgg
     8461 taaaaataga gcagaacgag ctatcaaggc caaacaatac caaaaagagc tggaaaaaga
     8521 ccaacgaatt gccagtcagg atccaattga aagaaaacag aagcagactt ggatcctcaa
     8581 ttcctatttt cgtgagcttc ctgaagaaaa agctcgattt tctagactct tattagaata
     8641 tcgaaaaagt ggtgaagtac ccttttcaac tgaatacctg tcagatcatc tcatcgactt
     8701 tttctataaa atgaaagcct ttgaatttga aatcgcacca gaacaagtcc gagattttct
     8761 aaaaaaaagc ctgcaggaag accatcgctc atcagcacaa ggaagctgga tagaaggaat
     8821 tctcctcaac tgcctcaaac catttttaga acgattagtg atatgagtaa cacctaccgt
     8881 gtaaatttca tggtaggtgt tattgtaccc ccaaataata taaaacaaag tatgaaagcg
     8941 catctcaatt ttaagacttt tttgtaaatt aagagtataa ttaaggtata ctgccttttc
     9001 tatagataat agataataca ctaatataat aaaggtcttt gtaacatata taaattttat
     9061 ttcaaggagg aataatggaa aagtattttg gtgaaaaaca agagcgtttt tcatttagaa
     9121 aattatcagt aggacttgta tctgcaacga tttcaagttt attttttatg tctgtattag
     9181 ctagttcatc tgtggatgct caagaaactg cgggagttca ctataaatat gtggcagatt
     9241 cagagctatc atcagaagaa aagaagcagc ttgtctatga tattccgaca tacgtggaga
     9301 atgatgatga aacttattat cttgtttata agttaaattc tcaaaatcaa ctggcggaat
     9361 taccaaatac tggaagcaag aatgagaggc aagccctagt tgctggtgct agcttagctg
     9421 ctctgggaat tttaattttt gctgtttcca agaaaaaggt taagaataaa acggtattac
     9481 atttagtatt ggttgcggga atgggaaatg gtgtcttagt ttcagtccat gctttagaaa
     9541 atcatctttt gctaaattac aatacggact atgaattgac ctctggagaa aaattacctc
     9601 ttcctaaaga gatttcaggt tacacttata ttggatatat caaagaggga aaaacgactt
     9661 ctgattttga agtaagtaat caagaaaaat cagcagccac tcctacaaaa caacaaaagg
     9721 tggattataa tgttacacca aattttgtag accatccatc aacagtacaa gctattcagg
     9781 aacaaacacc tgtttcttca actaagccga cagaagttca agtagttgaa aaacctttct
     9841 ctactgaatt aatcaatcca agaaaagaag agaaacaatc ttcagattct caagaacaat
     9901 tagccgaaca taagaatcta gaaacgaaga aagaggagaa gatttctcca aaagaaaaga
     9961 ctggggtaaa tacattaaat ccacaggatg aagttttatc aggtcaattg aacaaacctg
    10021 aactcttata tcgtgaggaa actatagaga caaaaataga ttttcaagaa gaaattcaag
    10081 aaaatcctga tttagctgaa ggaactgtaa gagtaaaaca agaaggtaaa ttaggtaaga
    10141 aagttgaaat cgtcagaata ttctctgtaa acaaggaaga agtttcgcga gaaattgttt
    10201 caacttcaac gactgcgcct attccaagaa tagtcgaaaa aggtactaaa aaaactcaag
    10261 ttataaagga acaacctgag actggtgtag aacataagga cgtacagtct ggagctattg
    10321 ttgaacccgc aattcagcct gagttgccag aagcagtcgt aaccgacaaa ggtgagcccg
    10381 ctgtccaacc tgaattaccc gaagcagtcg taaccgacaa agacgagcct gaagttcaac
    10441 cagagtcgcc agatactgtg gtaagtgata aaggtgaacc tgaacaggta gcaccgcttc
    10501 cagaatatac aggaccacaa gccggagcta ttgttgaacc cgcagtccag ccagcgttgc
    10561 ccgaagctgt tgtaagtgat aaaggtgaac cagagcaggt agccccattg ccagaatata
    10621 aggggaatat tgagcaagta aaacctgaaa ctccggttga gaagccgaaa gaacaaggtc
    10681 cagaaaaaac tgaagaagtt ccagtaaaac caacagaaga aacaccagta aatccaaatg
    10741 aaggtactac agaaggaacc tcaattcaag ta
//
LOCUS       DAVDWT010000052        10763 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVDWT010000052 DAVDWT010000000
VERSION     DAVDWT010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10763
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.052"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..219
                     /locus_tag="VUW50_001904"
     CDS             <1..219
                     /locus_tag="VUW50_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET7983738.1"
                     /translation="NSKPQADDKKPSTTNNLSKDKQPSNQASTNEKATNKPKKSLPST
                     GSISNLALEIAGLLTLAGATILAKKRMK"
     gene            complement(438..1418)
                     /gene="dusB"
                     /locus_tag="VUW50_001905"
     CDS             complement(438..1418)
                     /gene="dusB"
                     /locus_tag="VUW50_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET7983739.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKV"
     gene            complement(1405..2277)
                     /gene="hslO"
                     /locus_tag="VUW50_001906"
     CDS             complement(1405..2277)
                     /gene="hslO"
                     /locus_tag="VUW50_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET7983740.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2442..3869
                     /locus_tag="VUW50_001907"
     CDS             2442..3869
                     /locus_tag="VUW50_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7983741.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLFY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLVAIRQLLFT"
     gene            4028..5536
                     /gene="glpK"
                     /locus_tag="VUW50_001908"
     CDS             4028..5536
                     /gene="glpK"
                     /locus_tag="VUW50_001908"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET7983742.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5578..7404
                     /gene="glpO"
                     /locus_tag="VUW50_001909"
     CDS             5578..7404
                     /gene="glpO"
                     /locus_tag="VUW50_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HET7983743.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKSDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7474..8178
                     /locus_tag="VUW50_001910"
     CDS             7474..8178
                     /locus_tag="VUW50_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET7983744.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPISNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8369..8572
                     /locus_tag="VUW50_001911"
     CDS             8369..8572
                     /locus_tag="VUW50_001911"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983745.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRSELEDKKQELLNRLNK"
     gene            complement(8659..9267)
                     /locus_tag="VUW50_001912"
     CDS             complement(8659..9267)
                     /locus_tag="VUW50_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983746.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            10354..10635
                     /locus_tag="VUW50_001913"
     CDS             10354..10635
                     /locus_tag="VUW50_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturase"
                     /protein_id="HET7983747.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLRVEIPKPDGGIRQLGIPTVMDRMIQQAIV"
BASE COUNT         3187 a         2094 c         2442 g         3040 t
ORIGIN      
        1 aatagcaagc cacaagcaga tgataagaag ccatcaacta caaataattt aagcaaggac
       61 aagcaacctt ctaaccaagc ttcaacaaac gaaaaagcaa caaataaacc gaagaagtca
      121 ttgccatcaa ctggatctat ttcaaatcta gcacttgaaa ttgcaggtct tcttaccttg
      181 gcgggggcaa ccattcttgc taagaaaaga atgaaatagt gattgattca ttcaaaaatg
      241 tttgtaaaaa gtattcggct taaaagaaaa gagtctggga taaaaagatt ttgattttag
      301 gaattcttta ttataaattt ttaaaatcga tagattagac aaaaaagcga acaagacaga
      361 attctgagtg tcagataact gattttgttc gctttttata tttaatatta tacttttgtc
      421 ctaggctctt ttttgcatta tactttttcc aattgcaaga gagcttcaat ctctgctagg
      481 gtgctagctt gcgaaatggc tccacggagt ttggcagcgc cagatgttcc acggagatag
      541 tgaggagcga ggccgcggaa ttcacgaact gcgacgtttt ctcctttgag gttaatcaat
      601 cgtttcaagt gttcgtaggc gatcttcatc ttgtcttcaa aggtcaaatc aggtaggatt
      661 tctcctgttt caaagtaatg gttgatttgg ttgaagaggt aaggatttcc catggcagct
      721 cggccaatca tgactgcgtc agcaccaact tcttcgatgc gttgcttggc ttcttggaca
      781 gtacggatat caccgttggc gatgaatgga atcttggtta gagcttgggc aaccttgtaa
      841 agggtctcaa ggtctgcgtg gccagtatac atttgttcac gggtacggcc atgcatggcg
      901 agggcagaaa cacctgcagc ctcagcagcg agggcatttt ctactgcaag agatgggtcc
      961 gcccagccgg tacgcatttt gacagtaagt gggatatcaa ggacagactg gaccttgttg
     1021 atgatagagt agatcttgtc aggatccttg agccacatag caccagcttc gttcttcacg
     1081 attttgttga cagggcagcc catgttgata tcgacgatat cggtcttggt gttttcttgg
     1141 atgaattctg ctgcgcgtgc taggctgtct tcatcgctac caaaaagttg gatagagaca
     1201 gggttttcgc cctcatcgat atgaagcatg tgcagggttt tttcgttgtt gtattggatt
     1261 cccttgtcag agaccatttc cattacaacg agtccagctc cgagctcttt tgcgatggta
     1321 cgaaaggctg agttggtcac gccagccata ggcgctaaaa cggtacgatt gggaatctca
     1381 acattgccaa tcataaaagg tgtattaaga tttgtcacga atgagttcct ccaggtcctt
     1441 ttcatcaaag ttgtaagtag tttggcagaa ttgacaagtg atttctgccc cgtggtcttc
     1501 ctctttcatt tcctgtaagt ctgagcttgg aaggctggca agagcgttca taaagcgttc
     1561 atggctacag tcacattgga aacggatttc ttcttcagaa agacgcttgt aggcttcgtc
     1621 cccgtagata gccttgagga gggcttcgat atggtcgtcg ctttcgagaa gagtagagat
     1681 agctggcatt tcttggatgc gtttttcaaa gcgagcaatc tcttctttct tggctcctgg
     1741 caagacttga actaggaaac cacctgcaac cttgaccttg tcttcctcgt ccaaaaggac
     1801 attgaggccg accgctgaag gcgtttgttg gctttcagta aggtaaaagg caaggtcttc
     1861 accgatttct ccagagatga ggggagttat agagttgtaa ggatttccag taccgtagtc
     1921 tgtgataacg aggaattgac catttccaac aaaaggtccg actaggactt caccagtcgc
     1981 agtctttttg atgtcaacac caggattttg aacatagcct ttgacgttcc ccttggtatc
     2041 agcgacggtg ataatagcac ctagagagct agagcccaac accttaactg taagtttggt
     2101 atttcctttt tcattggctg cgagaatctg gctggcgata agagttcgac caagcgctac
     2161 agttgagcta gcttgggttt gatgtttttc ttgagcagtg cggacggttt ccgtgctatc
     2221 aaggacaaaa gcacgaaagg ctccgctttc tgatatagtt ttaataattt tatccatagc
     2281 tactatttta gcataaaaat gcccaaaggg ggagccgtgt gtttactgat tttcaggata
     2341 atggaccagg aaatcagcat gaaaataaaa agagaaacag attattttag catttgtaag
     2401 atttatgcta tgcttaaggt agaaaatgaa aggggtaaca aatgtattta ggagatttga
     2461 tggagaaagc cgagtgtggt caattttcaa tcctttcctt tctattacaa gagtctcaga
     2521 cgaccgtcaa ggctgtaatg gaagaaacag gattttcaaa agcaacccta accaaatatg
     2581 tcaccctgct caatgacaag gctttggata gtggcttaga actgactatt cactcagaag
     2641 atgaaaatct gcgtctgtct atcggtgcag ctaccaaggg gagagatatt cggagcttgt
     2701 ttttggagag tgctgttaaa taccagattt tggtttatct tttctaccac caacagtttt
     2761 tagcccatca gctggctcaa gaattggtga ttagcgaggc tacgcttggt cgtcacttgg
     2821 ctggtttaaa tcagattttg tcagaatttg atttatccat ccaaaatgga cgttggcgag
     2881 gtccagagca tcagattcgc tatttctatt tctgtctttt ccgaaaggtc tggtcgagtc
     2941 aggaatggga aggtcacatg cagaaaccag agagaaaaca ggagattgcc aatttagagg
     3001 aaatctgcgg tgcaagtttg tctgtggggc agaaattgga cttggttctc tgggctcaca
     3061 tcagtcaaca acgtcttcgg gtcaatgctt gtcagtttca agtcatagaa gagaaaatgc
     3121 gagggtattt tgacaatatc ttctatcttc gtttgctgag aaaggttccg tccttttttg
     3181 ctgggcaaca tattccacta ggagttgagg atggtgagat gatgatattc ttctcttttc
     3241 tcctatctca tcgcattctt cctcttcata ctatggagta tattcttggt tttggagggc
     3301 agttggcaga tttactgacg caattgattc aagaaatgaa gaaggaggaa ctattggggg
     3361 attatacaga ggaccatgtc acctatgaac tcagtcagct ttgtgctcaa gtctatctct
     3421 ataagggcta tattttacag gatcgctaca agtaccagtt agagaatcgt catccatatt
     3481 tactgatgga acatgatttt aaagagacag cagaggagat ttttcatgct ctacctgctt
     3541 ttcaacaggg gacagattta gataagaaga ttctctggga atggctccag ttaatcgaat
     3601 atatggctga aaacggtggt cagcatatgc ggattggtct ggatttgaca tctggttttc
     3661 ttgtcttttc aaggatggca gccattttga aacggtattt ggaatacaat cgttttatta
     3721 ccattgaagc ttatgaccct agtcggcatt atgatttgct ggttaccaat aacccgattc
     3781 ataagaagga acagacacca gtctattatt taaaaaatga cttggatatg gaggatttgg
     3841 tagcgattcg ccagttatta ttcacttaaa aggcttggtt aatccaggtc ttttttgtga
     3901 aattcacaca atctcctcac atttttttaa aaattaaaaa aagttgataa acaagaaagc
     3961 gctttatttt gtatactagt aagtgtaaag aggaaacacc tcaagatctt tatcaggagg
     4021 acagtacatg tcacaagaaa aatacatcat ggccattgac cagggaacta caagttctcg
     4081 tgccatcatt ttcaacaaaa aaggggaaaa ggttagctcg agtcaaaaag agtttaccca
     4141 gattttccct caagcaggtt gggttgagca caatgccaat gaaatttgga actctgttca
     4201 gtcagttatt gcgggtgctt tcatcgaaag tggtgtcaag ccaaatcaaa tcgaggcaat
     4261 cgggattacc aaccaacgtg aaacaacggt tgtctgggat aagaaaacag gacttcctat
     4321 ctacaatgct atcgtttggc agtcacgcca gacagcacct ttggctgagc aactaaaaag
     4381 ccaaggttat gtggaaaaat tccatgaaaa gactggtttg attattgatg cttacttctc
     4441 tgctaccaag gttcgttgga ttttggatca tgtagaaggt gctcaagagc gagcagaaaa
     4501 aggggaattg ctctttggta ctatcgatac ttggttggtt tggaaattga ctgacggtgc
     4561 ggctcacgtg actgactact caaatgcagc tcgtaccatg ctttataaca ttaaagaact
     4621 caaatgggat gatgagattt tggaaatcct taacattccg aaggctatac ttccagaagt
     4681 tcgttctaac tccgaaatct acggcaagac agctccattc catttctacg gtggagaggt
     4741 gccaatctca ggtatggctg gggaccaaca agcagccctc tttggacagt tggcttttga
     4801 gccaggtatg gttaagaata cttatggaac aggctctttc atcatcatga atactgggga
     4861 agagatgcag ttgtctgaaa acaacctctt gacaaccatt ggttacggaa tcaacggtaa
     4921 ggtttattat gccttggaag gttctatctt catcgcagga agtgctattc agtggcttcg
     4981 tgacggtctt cgcatggttg aaaattcacc agaatctgaa aaatacgctc gtgattctca
     5041 caacaacgat gaagtttatg tcgttccagc ctttacaggt ctaggcgctc catactggaa
     5101 ccaaaatgct cgtggttccg tctttggttt gactcgtgga acaagcaaag aagactttat
     5161 caaggcgact ttgcaatcta ttgcttatca agtgcgtgat atcatcgaca ccatgcaagt
     5221 ggatactcag accgccattc aagtactgaa ggtggatggt ggtgcagcca tgaacaactt
     5281 cctcatgcag ttccaggcgg atattttagg cattgacatt gcacgtgcta aaaacctgga
     5341 aacaacagct ctaggagcgg ccttcctagc aggtttgtca gtagggtact ggaaagactt
     5401 ggacgagttg aaactcttga acgagacagg agaactcttt gagccatcta tgaacgaatc
     5461 tcgcaaggaa caactctaca agggctggaa gaaggctgtg aaagcaactc aagtctttgc
     5521 ggaagtagac gactaatact ggcagaataa agcgatttat ttagaaagtg tgtaaatatg
     5581 gaattttcaa agaaaacacg tgaattgtca attaaaaaaa tgcaggaacg taccctggac
     5641 ctcttgatta tcggtggagg aatcacagga gctggtgtag ccttgcaggc ggcagctagc
     5701 ggtcttgaga ctggtttgat tgaaatgcaa gactttgcag aaggaacatc tagtcgttca
     5761 acaaaattgg ttcacggagg acttcgttac ctcaaacaat tcgacgtaga agtggtctca
     5821 gatacggttt ctgaacgtgc agtggttcaa caaatcgctc cacacattcc aaaatcagat
     5881 ccaatgctct taccagttta cgatgaagat ggagcaacct ttagcctctt ccgtcttaaa
     5941 gtagccatgg acttgtacga cctcttggca ggtgttagca acacaccaac tgcgaacaag
     6001 gttttgagca aggatcaagt cttggaacgc cagccaaact tgaagaagga aggcttggta
     6061 ggaggtggag tgtatcttga cttccgtaac aacgatgcgc gtctcgtgat tgaaaacatc
     6121 aaacgtgcca accaagacgg tgccctcatt gccaaccacg tgaaggcaga aggcttcctt
     6181 tttgacgaaa gtggcaagat tacaggtgtt gtagctcgtg atctcttgac agaccaagta
     6241 tttgaaatca aggcccgtct ggttattaat acaacaggtc cttggagtga taaagtacgt
     6301 aatttgtcta ataagggaac gcaattctca caaatgcgcc caactaaggg agttcacttg
     6361 gtagtagatt caagcaaaat caaggtttca cagccagttt actttgacac aggtttgggt
     6421 gacggtcgta tggtctttgt tctcccacgt gaaaacaaga cttactttgg tacaactgat
     6481 acagactaca caggtgattt ggagcatcca aaagtaactc aagaagatgt agattatcta
     6541 cttggcattg tcaacaaccg ctttccagaa tccaacatca ccattgatga tatcgaaagc
     6601 agctgggcag gtcttcgtcc attgattgca gggaacagtg cctctgacta taatggtgga
     6661 aataacggta ccatcagtga tgaaagcttt gacaacttga ttgcgactgt tgaatcttat
     6721 ctctccaaag aaaaaacacg tgaagatgtt gagtctgctg tcagcaagct tgaaagtagc
     6781 acatctgaga aatatttgga tccatctgca gtttctcgtg ggtctagctt ggaccgtgat
     6841 gacaatggtc ttttgactct tgctggcggt aaaatcacag actaccgtaa gatggctgaa
     6901 ggagctatgg agcgcgtggt tgacatcctc aaagcagaat ttgaccgtag ctttaaattg
     6961 atcaattcta aaacttaccc tgtttcaggt ggagaattga acccagcaaa tgtggattca
     7021 gaaatcgaag cctttgcgca acttggagta tcacgtggtt tggatagcaa ggaagctcac
     7081 tatctggcaa atctttacgg ttcaaatgca ccgaaagtct ttgcacttgc tcacagcttg
     7141 gaacaagcgc caggactcag cttggcagat actttgtccc ttcactatgc aatgcgcaac
     7201 gagttggctc ttagcccagt tgacttcctt cttcgtcgta ccaaccatat gctctttatg
     7261 cgtgatagct tggatagcat cgttgagcca gttttggatg aaatgggacg attctatgac
     7321 tggacagaag aagaaaaagc aacttaccgt gctgatgtcg aagcagctct cgctaacaac
     7381 gatttagcag aattaaaaaa ttaagaaaaa ataaaagagg tggagggcag cattccttgt
     7441 cgctcgtccc ttctttttaa tggagacaga aagatgatga atgaattatt tggagaattt
     7501 ctagggactt taatcctgat tcttctagga aatggtgttg ttgcaggtgt ggttcttcct
     7561 aaaaccaaga gcaatagctc aggttggatt gtgattacta tgggttgggg gattgcagtt
     7621 gcggttgcag tatttgtatc tggcaagctc agtccagctc atttaaatcc agctgtgaca
     7681 attggtgtag ctttaaaagg tgacttgcct tgggcttccg ttttgcctta tatcttagcc
     7741 cagttcgcag gggctatgct gggtcagatt ttggtttggt tgcaattcaa acctcactat
     7801 gaggcagaag aaaatgcagg caatatcctg gcaaccttca gtactggacc agccatcaag
     7861 gatactgtat caaacttgat tagcgaaatc cttggaacct ttgttttggt gttgacaatc
     7921 tttgctttgg gtctttacga ttttcaggca ggtatcggaa cctttgcagt gggtaccttg
     7981 attgtcggta tcggtctatc gctaggtggg acaacaggtt atgccttgaa tcctgcgcgt
     8041 gaccttggac ctcgtatcat gcacagtatc cttccaattt caaacaaggg agacggagac
     8101 tggtcttatg cttggattcc tgttgtggga cctgttatcg gtgcagcctt ggccgtgctt
     8161 gtattctcac ttttctaatc tagaaaacaa ttatgttgat agagcttggg caagagccca
     8221 atttcagcaa aaaatgaagt aaatcttctc ataataaaac gcatcatatc aagcacgaaa
     8281 attccacgag gtcaactaca gtcagaaagc tgaacaacaa gccaaaacgc ccaaaaaagg
     8341 cggcaaaaag caagcacctg caagcaacgt gccgaaatgg tcaaatcctg attatgtcaa
     8401 cgaattagac ccaaaaatcg ttgatatgct agtagaattt cacaagtcac aaggcacttt
     8461 ggaaactccc gaggcgcaag cagaaatcgc ccaaaaacgt gaagaaatcg agcaaaggag
     8521 atctgagctt gaggataaaa aacaagagct tttgaaccgc ttgaacaaat agagtttcgc
     8581 aagtattatg cttacaaatt acttgagcaa ttaactaaaa tataaaccct gcctttatat
     8641 ctaggcaggg tttatatttt agaaattcac gtaggttgtt acggttttta catacccagt
     8701 atagtttgag tttctatagt attcagtgat aaacttccat tttctttgag caacatggat
     8761 ataagtactt gttatgtagt atggatatgg gctttgtgaa tccaagtaag actgataagc
     8821 ttgtatacca aaatatgctc caccaattat tgcaccccat ggacccccca ataaagcacc
     8881 tatcctacca atcatataac tgattccagc accagtcatg aagttagcga atgtgttagc
     8941 ttgtttattc ccatgtattg tgttgacgta attccaaaca ttaggatcgt atgatctaaa
     9001 agatatattt aggtcgattt cattcttttg ataagccata taaaatgccc cattgatata
     9061 gacgccgtca gcacgtcgtt caatagtgtc tacacttcca tctggattga caacctcaag
     9121 aacttcatcg cttaaaatat ttacttgcgt atctccgaac cgcactgatg agccattctc
     9181 aaactgagcc tcaccagata caactttaga gtttgccgat aagctatcat cagcaaaaac
     9241 aacaagcgac ggggaaatgc tagacataca gaaaacagac ataactagca aacacatgca
     9301 tttaaacatc ttagacataa cggaaactcc tttgtatttt tgattttttt taacttttat
     9361 tatacaataa aaccaaataa aaagaaagcg gtaacaatat gcttaatgcg aaaatttttt
     9421 atatattttt atgtttgatc gttatcgaaa ctacaggctt gttgttgttg aaaagaggtc
     9481 tcgaaatggg ttatttagac acagaagcta ttatcctcgc agttttttca tttgcttttt
     9541 acaacctatg ttcattcgct tgggtctgct ctacaataaa aaacaataaa aaataaatag
     9601 acgtattttc aaaaaaaaaa atgaatattt atcttagcaa aatgacgatt taaatcgtcg
     9661 tttttttgta gtacgacggg catgtcgtat atctgaggtg taagtcctcg gtgggcacct
     9721 gctaccggtg aacccaatag cgattcccaa gcctgactat cgtgaggtag cagggagagg
     9781 aagggatagc gaaatcgtgg ctctacgaac aggaacgtga tagtaaggcg tatatagcgg
     9841 ataaggaggc ttcaaactct aaagtccaaa aaggtagtcg taacctatat gtgtaaatca
     9901 cgagagtaat tgaattcgga ctaaggtttg tgtgaaaaag ataaatcttt ctagagtcta
     9961 aagactctgc gtcagatttc ctattttcac tgtaaccttt taacgtcctc atatcttgta
    10021 taaacgagga aagatgtacg acttatcccg tgaggtttca tgagcgctga aagcgtagta
    10081 acaacgaatc atgagaagtc agccgagccc atagtagtga ggaaacttcc gtaatggaag
    10141 tggagcgaag gggtgaatac tcaaacagtc tggggagaga ctgtttgagg tctgtcgcta
    10201 gaaagagaaa acgacagatc gaagtaatcc tacttcactt gtgtctgtaa aatgagtggt
    10261 ctgatagaac tggactttga cacgtattga ctagatgaag gctcaccaat ataagatgtc
    10321 cctcaggcac caaaacaaga aaggaatacg cacatgtcaa aattgctaga taagatatta
    10381 tcacgcgaaa atatgctgga agcctacaat caagtaaaat ccaataaagg ctcagctggg
    10441 attgatggaa tgactatcga agagatggat aattatctca gacaaaactg gcgcttgact
    10501 aaggaactga taaaacagag aaaatataag cctcaaccag ttcttagagt tgagatacct
    10561 aaaccagacg gaggtatccg tcaactagga attccaacag ttatggatag aatgattcaa
    10621 caggccattg tctaagtcat gagccccatt tgtgaacccc atttctcaga tacgagttat
    10681 ggtttcagac caaataggtc atgtgaaaaa gccatcatga agctcttaga gtacttaaat
    10741 gacggctatg agtggatagt gga
//
LOCUS       DAVDWT010000053        10690 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVDWT010000053 DAVDWT010000000
VERSION     DAVDWT010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10690
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.053"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(22..1215)
                     /locus_tag="VUW50_001914"
     CDS             complement(22..1215)
                     /locus_tag="VUW50_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET7983748.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            complement(1353..2795)
                     /gene="gtfA"
                     /locus_tag="VUW50_001915"
     CDS             complement(1353..2795)
                     /gene="gtfA"
                     /locus_tag="VUW50_001915"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HET7983749.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKHLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            complement(3072..3152)
                     /locus_tag="VUW50_001916"
                     /pseudo
     CDS             complement(3072..3152)
                     /locus_tag="VUW50_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(3153..3986)
                     /locus_tag="VUW50_001917"
     CDS             complement(3153..3986)
                     /locus_tag="VUW50_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7983750.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            complement(4000..4866)
                     /locus_tag="VUW50_001918"
     CDS             complement(4000..4866)
                     /locus_tag="VUW50_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7983751.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIIAIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFFQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(4880..6139)
                     /locus_tag="VUW50_001919"
     CDS             complement(4880..6139)
                     /locus_tag="VUW50_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7983752.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(6231..8393)
                     /locus_tag="VUW50_001920"
     CDS             complement(6231..8393)
                     /locus_tag="VUW50_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET7983753.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPGYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLFYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGLVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTVSNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            8501..9361
                     /locus_tag="VUW50_001921"
     CDS             8501..9361
                     /locus_tag="VUW50_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET7983754.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKAPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            9358..10293
                     /locus_tag="VUW50_001922"
     CDS             9358..10293
                     /locus_tag="VUW50_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HET7983755.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            10544..>10690
                     /locus_tag="VUW50_001923"
     CDS             10544..>10690
                     /locus_tag="VUW50_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HET7983756.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINRDTHKEIITKLDYDAPSCP
                     NFGSQ"
BASE COUNT         3496 a         2248 c         1848 g         3098 t
ORIGIN      
        1 aagatttcaa acttataaaa cttaatccgt catgtccgat accaacattc gatgctccaa
       61 tggaatactg cacataacta gcaagaaaat aaagcctgac tgaatccaga agagagccaa
      121 gtcaaaaatt ccgtgcacag caaccactgt aaggaaagat agataaaggc cgataatcgg
      181 acgtttcccc gactcctgac tcatatccat catcaagcga acaggagcaa cagaagacaa
      241 aactaataaa atggtaccca caattccgta actcagaatc gtatcaatat aaagactgtg
      301 ggcatgttca tgataaggag catgtatccg aggataagag tgcatatagg tcaatggccc
      361 ttcaccccaa aaaggatttt gcttaaacaa ggccatccca gcatcccaga tagaaatgcg
      421 ttcttccata gaagagtcta aagtacccat tcgaactccc aaatcactag aaaagaggaa
      481 actcaaacca atcgcgaaga ccccaatact aagccaaaag gccttccagt ttttaatagt
      541 cgtaaagaga tagataattg ctccagcgat aatagcagga aaggcagttc gattttgagt
      601 aaagttcaaa ccaaagagat taacaaagcc tgcaatcaca cagaatactt tcaaccaatt
      661 caacttggtc gttgtaaaca gatagaaagc aatcataata cagaaacaac aaataattcc
      721 ataataatta ggattaaaga aggtcacttc tgcccggttc tgatgccaca cctgcatatt
      781 gggtgaaaga aaagcatagt taaatttctt cacaatttgg aaatgttcta aactggcaaa
      841 agcagctgac aagacactac caaacaagac gaactgcaaa atcaatcgaa agaatttatg
      901 ggataaaatc gactgatagt gcaaaaagaa aatagtaaat agaaacattc ctactgaagc
      961 cacaagaccc atccaatttt gtgcaagaat ggatataaca gtactatagc taagaaaaag
     1021 aagcagcatc ggatgctccc ccattttctg aagaatactt ttcatgtctc ctgtaaaaat
     1081 caaactgata atatataaac agagtacaac tacaaaaaga taaaagggta aaaagatact
     1141 caggataatt cccaataaaa tcagctcttt actagacaac cccttcagct tttcaataaa
     1201 gcctattgat ttcaaaatga atcctttctc tccaaatcag ctgattcaga taatagtaag
     1261 ctatcctata ttgtaccact tttttagcaa tttgaaaaca aaggaaacgt tttccaaaat
     1321 aaaaacccta ttttatccac catatcaagg cttcaaaatg atacttcaac tccattctca
     1381 attacccgat aagtctgatt ttgcaaatca atttctacta ctgctgttac ggacttatct
     1441 ttattttgac gtttgattac aatgctgtga gctgttggtg tctctatctc agtagtccct
     1501 tctagatcaa aggcttctga acggttacgg aaagaaaata gattgagaag ggccttcaca
     1561 acaggtcgtt gcacttcttt tgctatttcc tcgttgctat agtaatgacg attaatattt
     1621 cgaccttctt tagtttcttc taataatttc aagtcattct tgcctgctaa tagacccaca
     1681 tagtaaacct gaggaatacc tggggcaaaa gcttgaatta gacgagcgag aaaatacttg
     1741 acatcatcat ctccaagcgc tgaatagtag gttgaattga tttggtagat atctaagttg
     1801 ttatactcgg cactagagta cttacgtttg acattggctc caaccttata gagttcattt
     1861 gaagcatagt caatctcctc atcggtcagg atatccttga catctactac tccaatccca
     1921 tcatgggtat ctagcgtcgt aaattgcttc atcgggctca tctttaacca cttagccaaa
     1981 cgctctgttc tggaactgta aagagtataa agtgtcacca ttggaagagc aaaatcataa
     2041 acatagtaat catggtctgc tattttaaac tgaatcgaat agtgttcatg aatctcaggt
     2101 aaaagctctg tcccatactc agcagcgata tctcgaactt tgtccaataa atcccaaata
     2161 tctggttcca caaagaaatc attagtatcc aatttcttca ctgcataagc aaaggcatct
     2221 agacgaatca aatcacaccc attacttgcc aagtgctgaa tggtcttacg gataaattcc
     2281 atagttactt ctttggtcac atcaagatca atctgctcct caccaaaggt attccacaaa
     2341 tgttccactg aaccatcttc aaacacaatc tcttgctttg gtgcacgatc cttacgcttg
     2401 taaattaaat ctacatcaga ctgtgtcgga cggttttctg gccaaaactt atcccagttt
     2461 aaaaagagag ctttaaattc actggcttca tgtttttctt gatagtcctt ataatacttg
     2521 gattgacgag aaatatgatt aatcataaaa tcaaacataa gataatattt ctcacctaaa
     2581 tgcttaacat cctcccaatc accaaaagct gagtccactt cgtcgtagtc aactggcgca
     2641 aatccacgat cacctgttga tgggaaaaat ggtaaaaggt gaactcctcc aatagcatct
     2701 ccaaaatgct cttccaaatt atcatataag tctttaagat tatttccaag gctatcagaa
     2761 taggtaatca acatggtttt attttgaatt ggcatcatta ctctcctttt tctaattgaa
     2821 gccaagtctc atatgatctg gcttcataaa taaaattcat tttaaatctc tatttatcat
     2881 caaactcgta ctaatataga ctgtgataaa caaagtacta ctttcttgtt ttctgcatag
     2941 aattatcaac aagctaaact cttcctctgt gtcaaagact atagattcca tgagctcttc
     3001 ttatactctt cgaaaatctc ttcaaaccac gtcagcttca ccttgccgta ggtatggtta
     3061 ctgacttcgt cagtttcacc cacaacctca aaacagtgtt ttgagcaacc tgcggctagc
     3121 ttcctagttt gctctttgat tttcattgag tattacttca ctgccccgtt gctcattcct
     3181 gaaatgatat ggcgttggaa gaagagatag acaatggtga tactgataat gccgaccacg
     3241 taagaggcaa agcttggtcc gtagtcgttg aaatattggc ctgcgtagtt gtattggaac
     3301 aaaggcagag tccacatttt ggaatcccgg ttcaagacaa ggagtggcaa catgaagtca
     3361 ttccagaacc aaagggcatt gatgatcatg gttgtcgcat gcatcggttt catcattggg
     3421 aagatgatgc ggaaataggt tgtaaattga ttagccccat cgatctctgc tgcttcatcc
     3481 agactttctg gaatcgagat tttgatatag ccaacataga gaaagagggt ctgtggaatc
     3541 gcataggtca agtagagcaa gatcaaacca aaggtattag ccaaaccgag tttactcatc
     3601 ataaccgtaa tcggaatcat gatgacttgg aaaggtacga agattccgag gattaagagg
     3661 gtatacatga tggtaaaggc ttttctttta ctcatattgc gagcgatgga gtaggctgcc
     3721 atagggataa agatcattac tgcaagtaaa gacaagacag tgatgacgac agagttccaa
     3781 taatagcctc caatcccatc agctaagaga cggctaaagt tgtcccatgt gaagttggtt
     3841 ggaaagccaa agaaattatc tacaatatcc ttagtgggtt tgaaggaact aaagagggta
     3901 gcaaggagcg gcactaaaat cagaaccgat cctagaatca atagaatgta tttgccaatc
     3961 agggcttttc tttcatcttg tttcatcatg cttctcctct taaatttcaa atttcttaga
     4021 tactctcaat tggatgatcg aaatcactac aattaagaag aacaagatta cggcaatggc
     4081 attggcataa ccgaattggt tgtttttaaa ggcatagtta taaaccaaga gcccaagtga
     4141 ggttgtggca ttgtttggac caccaccggt catggcaaag acttggtcaa aggcagtcag
     4201 cccacctttt agggctagga taaagaccat agagacactt ggtagcaagt aaggcaattc
     4261 aatgttccag aaaacttgct tgctagtcgc accatcaatc cttgctgcct ctgtaatctc
     4321 agttggaata gattgcaaac cagctaggaa gatgatgatg ggcatagcca ccccttgcca
     4381 aagaaggaca aagacagccg caaagattgc tccccactta gtccctaaaa gactggtttg
     4441 gaaaaattca atatgaaggg catttccaat cgctggaaga ccgtagttga agacttgctt
     4501 gaagatcaaa gccactgtca aaccagataa aacagctggg aagaagaacc aagcacggaa
     4561 gaaggtttgg cctttgattt tagaattcaa gacacgcgca atgaagatcc cgagtgcaat
     4621 ctcaccaacc accatggcaa tcgcaatgat tgcggtaaag ccaatcgcat tcatgaattt
     4681 tggatccatg aagaggagct taaagttgtt taagccaaca aatttgtagt tataagtcaa
     4741 tcctgtccag ttggtaaaac tgtaaaaggc tccttgaaac atcggcacat agaagaaaat
     4801 tgcttgtaac aagaggggga tgaccacaaa agcccatgcc caatattttt gtaatacttt
     4861 tttcatagtc tctctactcc taatccacat ccgctttcat cgggttaaag aaggcattca
     4921 aatcattgac catgccttgt ttatcaccgg tcaagacata gttcatggtc aaggtatgga
     4981 agtctgcttc actggtccag tattgttgca accagaccaa gtgacgatcc gtaaaggcat
     5041 attcggtcat accagcaagc ggtgaatctt ctcctgcttg tttgacccct tcgatcgctg
     5101 ttggagatcc gtccacatcg tagtattttt gcatgacttc tggacgggtc atatattcca
     5161 caaaggcatt ggcttctttt ggatgtttgg tggtggctga gatagaccat gccaagtctc
     5221 ccgcaccaac ggttaagctt tgtccttttt cttttcctgg aatcatgaag gtcccaatct
     5281 taaagttcgg tttttgttca ttaatcgctg tgatcgccca agacccattt ggtgtcatga
     5341 ggacatcccc acgtgcgaag gctccgataa catcggtata gccagcacct tcccagttct
     5401 tttgcttaga gccattgatg cgaaggatgt ccatgacctt gatatcatct ttcataatcg
     5461 gatccgacaa tttaatggca tttggttgag aataacgaag gtattgattt gcttcttttc
     5521 ctccacctgt tgctgtcgca aaggctaatt gattgtaacc attgagtgtc caagcatctg
     5581 cacctgcaat tccaaatggt gtttgtcctt tagcaacgat atctttgact aactgttcaa
     5641 attcatccca ggtttcagga accttcaagc ccagttcttc gaatttatct ttgttgtagt
     5701 aaattccata agcattagct gtaaaaggaa cgttgtaaac tttttcgttt acagcatatt
     5761 tttcagcgta gccatttttc acgcgtttca ggtagtcttt gttgctcaaa tcttcaaaaa
     5821 cacctgcttt tgcccattct tgcagttcga tggactgtgg gtaaatattg accacatcag
     5881 gcacatctcc tgcgagaacg cgtgtcttca atacttcacc agcatttggt acattgacga
     5941 ctttgacttt gatcttaggg ttttccttct caaaatcacg agtgatttct tccaaggttt
     6001 tggtcatttc ttttttctgg ttgaaatact cgatggtcac tgtgccatcc gcagatttac
     6061 catagttgga gcaagcgccg agcccaaaca aagctaaacc tgtagttgca agaagtccga
     6121 tttttttata ccattccatt agaaagcctc ctttataaat ttatacaccc ttattgaact
     6181 gcaccccaaa agttagacag aataaatcta acttttgggg tcagtacata tcatagtttt
     6241 ctaaaaatat actgtctact caaaaaatct ccttgggata agataacagt taagcccgca
     6301 tacattagtt ctgcacctga gtaaacttcg ccattttcct gtaatttata tagtccctct
     6361 tcatccaaat cttttaattt taaagttgtt tccatggtct ctacaacaga taaaacgcga
     6421 acgtaggtta caatcgtttg atttccgtaa ttaaattgta cagctgcttc attggataca
     6481 gtatcaggat taattagtct atactgctgt cctaactgaa ctactggtcg taattcttta
     6541 tacaagttca cctgattagc aatcgtagct ttctcttcat ctgataaatt tgtcaaatca
     6601 agttcatagc ccaaatttcc catcattgct acaaggccac gtgtttctaa tggtgtcatt
     6661 cgtcccatct gatgattcgg tactgctgac acatgagccc ccatagaaat ggttggatag
     6721 agataggatg aaccgtattg aattggtaaa cgtgcaatgg catcagtatt atcactagcc
     6781 cagacttgtg ggaaatagcg catcatacca agatcatttc gtccaccacc accagagcag
     6841 gactcaaaga gaatatggct gtgcttctct gtcagataag aaacgagttc ataaagcccc
     6901 agcatgtact gatgagattg catctgtgtc tctagataag ttaatccatt ccctagctta
     6961 gttatattgc ggttcatatc ccatttaatg taatcaatat catgataaaa taggagttga
     7021 tctaagacac ttttcaagta ttctactacc tgaggattgg caagattaag tactaattga
     7081 ttccgagaat aagtatgctc atagccagga acctgaatag cccagtcagg atgttgacga
     7141 tacaaatcac tatctacaga aatcatttcg ggttctaacc aaagtccaaa ctgcaaacct
     7201 ctttcatgga tagctgaaat cagactttct agacttccac ccagtttttc ctcattaaca
     7261 acccaatcac ctaaagcacg attatcatca aaacgattgc caaaccaacc atcatctaat
     7321 acaaaaagtt caatgccaac tttcttagct tcatctgcta actctaacag tttttctctc
     7381 tgaaagtcaa agtaagtagc ttcccagtta ttgattagaa ttggacgttc ttttttagaa
     7441 aattcactta gcataatgtg cttcagtaca aaattctgac tttcatgact aataccagtt
     7501 aatccctgat ctgaatgagt cactaaagct accggtgttt caaagtattc ctcaggagct
     7561 aacttccaag aaaagttttc tggattaatg ccaatagcca cccgaacttc attcaattga
     7621 tttttttgaa caaaagcttc aaagttgcca ctatacatta gttgaatagc aaacacattc
     7681 ccagcatcct ctgtgactcc ttgttcgcat agtagaagag ctggtgtttg agcatgacca
     7741 gaagcacctc ggtttgaact aatcgaaaag attccttgtt ctacctgttg acgtctaaca
     7801 gtcttttcac gagcataagc accctgcaga gttactattt cgtaatctgc agctggaaaa
     7861 tcagccataa aagaaaaatc tttatggatg acaacttcct gattactatt attatctaat
     7921 ttactgtagc tagcaatagt cgcatcatta ttaaaagtag tataatacaa agtcagacta
     7981 agttgagcct tagaatcttc taacattaag acaagagtct ctgtatcgtc catgctatgt
     8041 ggagaaggta agccctgtgg accattctga ccttttaaaa tctttgcttc tacaaatcga
     8101 aagtctgtta cttcagttac actatgctga acctgtatgg ttggtttcct aaaatctcct
     8161 aagccatgtt gtccaaaaat ctgtcgctga gtatctaaac taaaggttcg attagtagcc
     8221 gttggatttc ctgaaaaggc atggtctcgt tcataaacac tattggaacc tttatagttc
     8281 ttaatagtct ttcctaaatg tttcaaaagt aagtagccat ttcgattttc aataatcaaa
     8341 cttagatttt tactctcaac ataaaataga ttattctcta tcctaactcc catttacttc
     8401 acctcatcac tttattgatt atattttatc acctgaaatc gctttccaaa atagaaaaat
     8461 gtctcaagaa tatggtaaaa tgttaggtag gaggtagcac atgttagttt tttcagaata
     8521 ccagactgga acaatcgacc ttgccctaag cttttatgga tatgaggaat gcacacctaa
     8581 ttactctttt ggtccagcca ttcgtgatac atacgttcta cattacatta ctaaaggaca
     8641 aggaaaattt cattacaagg gtaaaattgt tgatttaaaa gaaggagatt tctttctatt
     8701 aaaaccagag gaactaacct tttatcaagc agatagtaaa gaaccttggg cctactactg
     8761 gttaggaatc actggaggga aagcccctga ttattttgct ctttcccaaa tttctgatca
     8821 atcctatctc atccaatctg aaacttgtca tacccagact actgcaaaac tcatctcaga
     8881 cattgtccgc ttcgctcaga ttacaaaatc aagtgaatta gctcaactcc atatcatggg
     8941 acaacttcat gaactgatgt ttcatctggg aactattgct cccaatcaga aaaaaaagaa
     9001 tatttcatca acccaccaac tctatcttga atgcaaacga ttaattgata gccactatcc
     9061 tcaatcactt acaattcaag atttagcaaa agaactatcc gttcacagaa gctacttatc
     9121 aagcgtattc aaagaattta ataccttatc acccaaagaa tacctactct acgttcgaat
     9181 gcaccgagct agacaacttc tcgaaaatac ccaagagtcc atcaaggtaa ttgcatactc
     9241 ggtaggtttt tcagatccac tccatttttc gaaagcttat aaacaatact ttaatcagac
     9301 tccaagtcat acaagaaaag aatactctca ataccaacta gtaagaaagg caacattatg
     9361 aaatcctacc aagctgtcta ccaaatccta tctaaagaaa ccgactatat cagcggagaa
     9421 aaaatcgcag aaaaactatc cctaagccga acagcaattt ggaaagccat caagcgacta
     9481 gaacaagaag gcattgaaat tgatagtatc aaaaatagag gatataaact gatgaatggt
     9541 gaccttattc ttccagagat tctagaagaa aatcttccaa ttaaagtcag ctttaaaccc
     9601 gaaacaaaat caacacaact agatgcaaaa gaagcaattg atttaggcca tgaagcaaat
     9661 accctctatc tagcttccta tcaaacagca ggccgaggcc gttttcaacg ttccttctac
     9721 tcaccacaag gtggtattta tatgacactc catcttaaac caaatctccc ctatgacaaa
     9781 ttaccatcct acacactact tgtagctgga gctgtctaca aagccattaa gaacctaact
     9841 ttaatagatg tcgacataaa atgggtcaat gatatctatc taaacaatca taaaattgga
     9901 ggaatcctta ctgaagcaat gacctctgta gaaactggct tagtcacaga tatcattatt
     9961 ggagtaggta tcaatttcac tattaaagac ttccctcagg aattaaaaga aaaagctgcc
    10021 agcttattta aagctacagc tcctataaca aggaatgaat tgatcataga aatctggcgt
    10081 gctttcttcg aaacaccagc agaagagcta ttatacctat acaaaaaaca gtcattcatt
    10141 ctaggaaaag aagtcacttt cacactagag caaaaagact acaagggact tgctaaagac
    10201 atctcagaaa atggaaaact tttagttcaa tgtgataacg gaaaagaaat ctggctaaat
    10261 agtggcgaaa tttctctcaa tagttggaag taaaataaca caattataat ataaacgata
    10321 taaaaataac ttcagattag taattcaatt aagttttacg gatctgaagt tttattggct
    10381 ctaaaaataa aaaagagagt tacaaactct cattaaaacg gctctataat atttgtagtg
    10441 ggtaaatccc ctatggatat tatggagctt atttttgtgt agaaaaaaag tcccatatga
    10501 cctataatga aaagcgacaa aacaactcat tagaaagaat catatggaac aattacattt
    10561 tatcacaaaa ctgctcgata ttaaagaccc aaacatcaag attctagata tcatcaatag
    10621 ggatacacat aaggaaatca tcactaaatt ggattatgat gccccatctt gtcctaattt
    10681 cggaagtcaa
//
LOCUS       DAVDWT010000054        10106 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVDWT010000054 DAVDWT010000000
VERSION     DAVDWT010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10106
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.054"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            72..1331
                     /locus_tag="VUW50_001924"
     CDS             72..1331
                     /locus_tag="VUW50_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HET7983757.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKALGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    1407..1542
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1581..2081
                     /locus_tag="VUW50_001925"
     CDS             1581..2081
                     /locus_tag="VUW50_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET7983758.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            2157..2525
                     /gene="rplL"
                     /locus_tag="VUW50_001926"
     CDS             2157..2525
                     /gene="rplL"
                     /locus_tag="VUW50_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET7983759.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            2724..2897
                     /locus_tag="VUW50_001927"
     CDS             2724..2897
                     /locus_tag="VUW50_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983760.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQS"
     gene            2894..3142
                     /locus_tag="VUW50_001928"
                     /pseudo
     CDS             2894..3142
                     /locus_tag="VUW50_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            3156..3479
                     /locus_tag="VUW50_001929"
     CDS             3156..3479
                     /locus_tag="VUW50_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983761.1"
                     /translation="MTFLDDYHKKHNYPLFYESYLQNVMEFLESQDIKNGADAFVDDH
                     QNLVFVLYGQGYRAEGKEGILTTQVTVKAYDEDKQPINFANLLDSLIVSEYQMEPNIW
                     EVSHD"
     gene            3472..3852
                     /locus_tag="VUW50_001930"
     CDS             3472..3852
                     /locus_tag="VUW50_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET7983762.1"
                     /translation="MIDLYLSKNSQRNQLLLDFFQNYGIEVSCHSVSEMTRDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLVVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLYN"
     gene            3865..4008
                     /locus_tag="VUW50_001931"
     CDS             3865..4008
                     /locus_tag="VUW50_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983763.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            4101..4688
                     /locus_tag="VUW50_001932"
     CDS             4101..4688
                     /locus_tag="VUW50_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET7983764.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRNLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            4890..5027
                     /locus_tag="VUW50_001933"
                     /pseudo
     CDS             4890..5027
                     /locus_tag="VUW50_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
     gene            5317..6720
                     /locus_tag="VUW50_001934"
     CDS             5317..6720
                     /locus_tag="VUW50_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET7983765.1"
                     /translation="MNNDEWVYQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICH
                     EMRIPFKHLSTEDKFIMRNGKLVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGP
                     YILSDKRWDEAPIYLRYGVFRPSRDDEKKVVIDELIVGDEVVKDERLPVFKIPKGIVP
                     PDFLNKWLDKKDKKQGDFPFIIDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLG
                     FDGTYSSERLASECKALKILNEWSEMPKIYWYGKIWEHTFLGIEHMKGVPLNRWVTNN
                     FPLYEVVDKTKDYLLRVSKIVEKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIID
                     WEQAVFSNDEKVVHKVAAPGFRAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNY
                     VSQTRIEGKKLFESLGYTREHIDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIE
                     SEQDIQDFIIKAFLLVN"
     gene            6909..7527
                     /locus_tag="VUW50_001935"
                     /pseudo
     CDS             6909..7527
                     /locus_tag="VUW50_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656069.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            7542..8282
                     /locus_tag="VUW50_001936"
     CDS             7542..8282
                     /locus_tag="VUW50_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7983766.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            8285..9604
                     /locus_tag="VUW50_001937"
     CDS             8285..9604
                     /locus_tag="VUW50_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7983767.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASRLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQMSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIVSKWNLLYCKSY
                     IKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIIS
                     IAFLSSHQERTEIQQFEILGTSNYQMVKIKVGEAIFLTFVTSLIAFLLNIKIIALIYY
                     SLEDILIDDMNLLGLILPNFIVSIILFILIFITKSSYFIFKNAKIIS"
     gene            9738..9875
                     /locus_tag="VUW50_001938"
     CDS             9738..9875
                     /locus_tag="VUW50_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HET7983768.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDEMRFSTFSTNRCGNWSAFSWEN
                     C"
BASE COUNT         3343 a         1472 c         2023 g         3268 t
ORIGIN      
        1 acacctacgg caaagtgaat ctgaagtggt ttgaagagag tacaacttgt cttttagaaa
       61 aggagcctat aatgaaagtc tttcagcatg taaatatcgt gacttgtgat caagatttcc
      121 atgtttatct tgatggaatc ttagcagtca aggattctca aatcgtctat gtcggtcaag
      181 ataagccagc gtttttagag caagctgagc agattataga ctatcaggga gcttggatta
      241 tgcctggttt ggtcaattgt cacacccatt ctgcaatgac aggtctgaga gggatccgag
      301 atgacagcaa tctccatgaa tggctcaatg actatatctg gccagcagaa tctgagttta
      361 ctcccgacat gactaccaat gcggtcaaag aagccctgac agagatgctc cagtcaggaa
      421 caacaacctt taacgatatg tataatccca atggtgtgga tattcagcaa atttatcagg
      481 tggtgaaaac ttccaagatg cgttgttatt tttctccgac tctcttttct tcagagacag
      541 aaacaactgc tgagactata agcagaactc gatccatcat agacgaaatc ttaaaatata
      601 aaaatccaaa tttcaaggtt atggtagcac ctcattctcc gtatagctgc agtagagact
      661 tgctggaagc gagtttggaa atggcaaaag agctaaatat tccgctccat gtccatgtgg
      721 cggagaccaa ggaggagtca ggaattatcc tcaaacgata cggcaaacgc ccccttgctt
      781 ttcttgaaga actgggttat ttagatcatc cgtccgtatt tgctcacggg gtcgaattaa
      841 acgagagaga aattgaacgc ttggcatctt ctcaagtggc tatcgcccac aatcctatca
      901 gtaacctcaa actggcatca ggaattgctc caattatcca gctccaaaaa gcgggagtag
      961 cagtcggaat tgcgactgac tcggttgctt ccaataacaa tctagatatg tttgaggaag
     1021 gaaggactgc agctcttctt cagaagatga aaagtgggga tgccagccag tttccaatcg
     1081 aaacagctct caaggtactg acaatcgaag gggctaaggc ccttggaatg gaaaatcaga
     1141 taggaagtct ggaagtcggc aagcaagcag attttctggt cattcaacca caagggaaaa
     1201 ttcatctcca acctcaggaa aatatgctgt ctcacctggt ttatgcagtt aaatctagtg
     1261 atgtagatga tgtttatatc gccggagaac aggttgttaa gcaaggtcaa gtcctgacag
     1321 tagaacttta aaagaaaaat cacgaaaaat tttaaaaaaa gtttgcaaaa atcttgcatt
     1381 ctttttttga ctatgctata cttatatacg gtttaaaaaa actgcctaag acagtagggg
     1441 agctcgactc ataaatatcc taccgaggac aagacgtatc atgtaaaaag aagcgtattg
     1501 tactttcgtg tctaggtttg ggcgcgtttt tctttttgaa aaattcccca agcaaaataa
     1561 ttacggaggt gaacacacta atgagtgaag caattattgc taaaaaagcg gaactagttg
     1621 acgtagtagc tgaaaaaatg aaagctgctg catctatcgt cgttgtagac gctcgtggtt
     1681 tgacagttga gcaagataca gttcttcgtc gcgagcttcg tggaagcgaa gttgagtata
     1741 aagttattaa aaactcaatc ttgcgtcgtg cagctgaaaa agctggtctt gaagatcttg
     1801 catctgtatt tgttggacca tctgcagtag cattttctaa tgaagatgtt atcgcaccag
     1861 cgaaaatctt gaacgacttt tctaaaaacg ctgaagcact tgaaattaaa ggtggtgcaa
     1921 tcgaaggcgc tgtcgcatct aaagaagaga ttcttgcact tgcaactctt ccaaaccgcg
     1981 aaggacttct ttctatgctc ctttctgtac ttcaagcgcc agtgcgcaac gttgctcttg
     2041 cagtcaaagc ggttgcagaa agcaaagaag acgcggctta atcttaagct acacagcgta
     2101 gcctagctac gaaaaaaact attataaaat ttaaaactta tttggaggaa ataacaatgg
     2161 cattgaacat tgaaaacatt attgctgaaa ttaaagaagc ttcaatcctt gaattgaacg
     2221 accttgtaaa agctatcgaa gaagaatttg gtgtaactgc agctgctcct gtagctgttg
     2281 ctgcagctga tgcagctgat gctggtgctg ctaaagattc attcgacgtt gaattgacat
     2341 ctgcaggcga caaaaaagtt ggtgttatca aagttgtacg tgaaatcact ggtcttggtc
     2401 ttaaagaagc taaagaactt gttgacggtg caccagcact tgttaaagaa ggcgttgcaa
     2461 ctgcagaagc tgaagaaatc aaagctaaat tggaagaagc tggagcttca gttactctta
     2521 aataagaggc atataccaat tagaattcgg aatactatag tatcagcctt ttagaatcgt
     2581 gaacacattt tagaaactga taaatttatt tagtttccta ccaaaaaccc taccaaaaat
     2641 tctttggcag ggtttttgtt ttaataaact ttcgggggta gacactagat tgtctaccct
     2701 ttttttgtgt ttcggaggta attatgcaaa aggaaggaca gtcatcacgt caattcgtga
     2761 agagtcatct catgactatc atgggagttg atattgtaaa agcaactcag ttagttgatg
     2821 aaatggaaca agtaggttta atccgatttg atgaatttgg aaatgttgga attttagtct
     2881 tggagggaca atcatgaagc gtattaccgc tagtcaatat cagacatcag agcgttatta
     2941 taagctcccc aaacttttat ttgagagtga acggtataag aatatgaaac tggaggttaa
     3001 ggtggtttac tcagttttaa aagatcggtt ggagttgtct ttgagcaaag gttggattga
     3061 tgaggatggg gctatttatt tgatttattc caattcaaat ttgatggcac ttttaggctg
     3121 ttcaaagtca aaattactct ccatgtgagt ttgaagtgac atttttagat gattaccata
     3181 aaaaacacaa ctacccccta ttttacgaat cctaccttca aaacgttatg gaattccttg
     3241 aaagtcagga tataaagaac ggagctgatg cctttgtaga tgatcatcaa aatcttgttt
     3301 ttgttttata tggacaaggc tatcgagccg agggaaaaga gggaatactt acaacccaag
     3361 taactgtaaa agcttatgat gaagacaagc aaccgattaa cttcgcaaat ttattagatt
     3421 ccttaatcgt atcagaatat caaatggaac cgaatatttg ggaggtctcc catgattgat
     3481 ctctatctaa gtaaaaatag ccaaagaaat caacttcttt tagacttttt ccaaaactat
     3541 ggtatcgagg tatcttgtca ttcagtttct gaaatgacaa gggataaatt aattgagatg
     3601 atgagttatt cttcagattg ttttgagttt ttatctccca atttattgcg atttaagaac
     3661 cgtgacaatc taagattaac ggatttcgtt gagataattt taaaaaatcc tgaactaacc
     3721 atcagacttc ctcttgtggt ttcaaataag cgggtttatc caagtctgaa tttggaagag
     3781 gctagagctc tattgccaag agatacgaaa caattgattt acatggcaca aacacattat
     3841 ttatataact aaaggagaag caatatggct gaacgatttt gggagaactt gtccattatt
     3901 ttggctgaaa gaaatatcag ctggattgag ttaaccagaa aaatgtttgc gggagagttt
     3961 cactatccaa gtgaattgaa tcgtttgtat caaaagattc gccactagaa gatggaacaa
     4021 tgaatgcctc aaagtccatg ggtagaaagg attgtgcagg tattagattt agattatgaa
     4081 gatttatttc ggaggtaact atgaaaagaa taattccagt ttatatattc caacaagtaa
     4141 atgtcctatt ggtatctcta tacttattga aatttctttg tatcggtgag ttaactatac
     4201 tacagattct ctatggttcg tcactcattt cttttttatg gatgtatggc caacgcaaac
     4261 aagcgcataa ggtcaatatg aaatctagga tgaaatggct tggtgttgaa tttgttagcc
     4321 tactgattat aagtctatgt tttagtctga ttcatgctca aggaagcacg aatcaagcaa
     4381 acttaattgg ccttcaacat caaattcctt ggttttcatt tttattgttc ttaatcaatg
     4441 cgagtatggt tgaagaattt ctgtatcgag aaattttatg gaatttggtc agaaatttag
     4501 atattcgagt tgctttgaca agtgttttat ttgccttagc acatcataca ggaaccattc
     4561 tagcttggtg tttgtatgtt tcacttggga tgtttttagg tatggtgcgc tataaatctg
     4621 acttatgggg cagtatggga ctacatttgg tgtggaacct actagtttat agtttgctgc
     4681 ttttttaaaa taggtaacct gatttttagg taacttctaa aactaacttc cagttttggg
     4741 agagagatgg aagttacttt gaaaagttac ggaaacatta ttttctatgt gacaacattg
     4801 tatcattgct tgcattttac tgctggagag agtataatga aggtggataa agcacttaat
     4861 cctaaaaatt taaataggag gagttagcta tggcagaaga agtattaaat ctttaattag
     4921 tatctgttca agttgacgaa acagatgaag ttgatggaat gcgatttagt acattcagta
     4981 caaaccgttg cggtaactgg agtgctttta gttgggaaaa ttgttaattc atttaaattg
     5041 taaactttag agattctctg gagttatgga tctcgttcca atatgtgcac gttggaatgt
     5101 cagtgcttat atggggggaa tccaaaggat tctccctttt tattttaata agtctgtcta
     5161 gctatagata tattaagttt gatagaattt cttggagatt ttgttatgga ttataatttc
     5221 aatttggagc atcctttctt tttcactaat aatgattata gtacagatac tagcataaag
     5281 tactaagtaa gtctgccttt taattggcac gaagtaatga ataatgatga gtgggtttat
     5341 caatacccta ttggaaaatt tgttgaaaga caagggtgga aaatacacat tagttcagag
     5401 tataatagtt cacacgaatt gttacaagat gtagcaaaaa tttgccatga aatgcgaatt
     5461 ccatttaaac atcttagtac agaagacaaa tttatcatgc gaaacgggaa attggttagt
     5521 cgaggatttt ctggtaaatt tattacatgt tatccaaatc aaaatgaatt agagtcggtt
     5581 ttgcaaaggt tagaaagtgc cttaaagcaa tataatgggc catatatttt aagcgataaa
     5641 cgttgggatg aagcaccaat atatctgagg tatggtgtgt ttaggccgtc aagggatgat
     5701 gaaaagaagg ttgttattga tgaattaatt gttggcgatg aagttgttaa agatgaacgt
     5761 ttaccagtat ttaaaattcc aaaaggtata gtgccccctg actttttgaa taaatggctt
     5821 gacaagaaag ataaaaaaca gggagacttt ccctttatta tagataatgc aattagattt
     5881 tcaaacagtg gtgggatata taatgctaga ctaaaagaag atgggaaaaa aataatttta
     5941 aaagaggcta gaccgtatac aggtctagga tttgatggta cttattcttc agagagatta
     6001 gcttcggagt gcaaagcact aaaaattcta aatgaatgga gtgagatgcc taaaatatac
     6061 tggtacggta agatctggga gcacaccttc ttaggaattg aacatatgaa gggcgttcca
     6121 ctaaatagat gggttaccaa taattttcct ttatatgaag ttgttgataa gaccaaagat
     6181 tatctattga gagtttcaaa gatagtagaa aaactcatcg atttaacgaa taagtttcat
     6241 tctgaaaatg tatatcatca agatttacat ttaggaaata tcttggttaa ggatgaagat
     6301 gagattagta taatagactg ggaacaggct gtcttttcga atgatgaaaa agttgtacat
     6361 aaagttgcag cccctggttt tagagcatgg cgagaaacac ttcccagtga gatagattgg
     6421 tatggtatta gacaaatagc tcattattta tacatgccac tagtaactac atcggatttg
     6481 acatacaact atgtaagtca aaccaggata gaaggaaaaa aactatttga gtctttgggt
     6541 tatacaagag aacatataga ttatgtagaa agtttattgt catatctaga ttctaaatgt
     6601 ccacagattg agaatatttc caggaaaaaa gttttaaaac ccatgcatga gattagaact
     6661 atcgagagtg agcaagatat acaagatttt attataaaag cttttctttt agtgaactga
     6721 taggtatttt cttagcaaac gataaagttt tagggccgat acaatcaatt gcctattcat
     6781 taaataagat aaatacaacc aaagatttaa ggaaaccgtt tttaaaatac ttaagtggag
     6841 agaagaattt tatagacgct gaacatgata ataacggact gtatacttca tcaatagatg
     6901 agatacacat gaaagatgtt gtatattcta ttacaccaga aaataaatta agtattgact
     6961 tctcatttaa gtcaccattt agggtattat taacaggaac ttctggtagt gggaaaacaa
     7021 cgattttaaa tttaattaat ggttctttaa agccacaaaa aggttatgta aatttgttat
     7081 cacatgggaa aaagagttca gattcaatac caacagttga tcagacacca tatatttttg
     7141 acactactat tcgtgagaac gtaactttat ttcaaaatga atatttttca gatgatcaga
     7201 taattgaggt gttaaaaaag gtaaatctat atgaagaatt agaaaagata gatatactaa
     7261 attatcaatg tggtgaaaat ggtagtaatt tgtctggagg tcaaaaacaa aaaatagctt
     7321 tagctagagc tctgattaga aataataaag tgtacttatt tgacgaaata tcagctaatt
     7381 tagataatga taattcaaat tccatacatg atattctgtt caatttaggt atttcattta
     7441 ttgaagtttc acatcattat gacttaaatg acaagagata cactgatata taaattggaa
     7501 aatggaacac ttttcaaaat taagtgattg gaggcaagaa tatggataaa gattatatat
     7561 taaaagtgaa agggctgtat catcaatttt tactgggaaa caataaaacg ttgcaagtgc
     7621 tgaaaaatgt ttctctttct gcttcgagag gagaatttat aagtattcta ggaattagtg
     7681 gttctggaaa gtcaacttta ttaaaatgta tttctagttt gcttgaacca acaagtgggg
     7741 aagtaatttt aaatggaatc aacccctata aaatcagaaa tgcaaaatta tcaagtataa
     7801 gacgtaatga agtatctttt atatttcaag catataattt aataccttcc ctgccggtaa
     7861 tagaaaatat agcacttcct ttgcgattat cacaaaaaaa attaactatt aaaaatgtag
     7921 aaaacttact caaaagaatg aagtttaatg ctggcttaaa cgattttgtt ggaactctgt
     7981 ctggtggaga acaacagaag gttgctatag ctagagcggt tattgctgat agtgatataa
     8041 tatttgctga tgagccaact ggggctttag acagcgtttc tcgtgaagta atttttgaat
     8101 tattgagaga gttagtaggg gcgggtaagt gtgtaattat ggtaacgcac gatatagaat
     8161 tggcctcgaa aactgatcgt gcattaatat tgaaagatgg aaaaattttc aaagaacttc
     8221 atagacctag cggggaagag ttgtataaaa tcttagaggt acaatcaact acggaggaat
     8281 agagatgctc agattagttt actatcaatt tttacataat aagaaacaat ggttaggcgt
     8341 ttcaccagta atttttgttt cgagtttagt tatgggattg gctgttaatg gggtaattaa
     8401 tgttgagaat aattcgcaag tatttgtcgg acttccagat cctaaaccaa tttttatgtt
     8461 tccgatagta tttggaggag taacattatt ctttgtgctc tctaacataa taaatatgtt
     8521 ggttgaaatt tttagagatg attacgaact tctggaggtg ttgggagcaa gtcgccttca
     8581 actatcattt cttgtggggg gacagatttt tatcatttcc tcgattatta gttttattgc
     8641 atatctatgc tctatatttg ttacgtcaaa ttattattat tttttacaat actttttcgg
     8701 ggaaaatata ttacctgata tccaatttca aatgagtgct gttggatgta tcattacagt
     8761 agtgttgatt tcatttttag cctttctaag tggttgcttt tatacgttca agaagattag
     8821 aaataggaag agttctaaaa taaggcatgt tctttcaata gtaaaaagaa tactgctatt
     8881 ggctggtttt agtgttatct ggttgctttc tttgcaacaa attttccaag atagcacaat
     8941 tttagcgaag gcccagataa tattcaatat tgtaatctta gatattgtga ttatttatca
     9001 attatctcct tttatacagt catgttttat caaactactt tctataatta tatttagaaa
     9061 taattttatg ttcattgttt cgaagtggaa tttactatat tgtaagtctt atataaaatc
     9121 tatcagtgct gcaattacag gagctatatt actaatttct agttttcaaa tgatttctca
     9181 gaatatacta tctcaatttc aagatgacag tgatttagag ctaaaagtgg catttattgt
     9241 ctatgtagga gcaccaattt tgatagtact tgcaaatatt atttcaattg catttttgtc
     9301 atcgcatcaa gaaaggactg agattcagca atttgaaatt ttaggaactt ctaattacca
     9361 gatggttaag attaaagttg gtgaggctat atttttgaca tttgttactt cattaattgc
     9421 atttttactt aacattaaaa ttattgcttt aatatactac tctttagagg atattttaat
     9481 agatgatatg aatcttttgg ggttaatact tccaaatttt atagtatcta ttattttatt
     9541 tattttaata tttattacta aaagttccta ttttatcttt aagaatgcaa aaattatttc
     9601 ttaaaaattt gtaagttttc gctatataaa atagttaaat ctattttgta taattgcttg
     9661 cattttactg gtggagagaa tataatgagg gtggataaag tacttaatcc caaaaattta
     9721 aataggagga gctagctatg gcagaagaag tattaaatct tcaattagta tctgttcaag
     9781 ttgacgaaac agatgaagtt gatgaaatgc gatttagtac attcagtaca aaccgttgcg
     9841 gtaactggag tgcttttagt tgggaaaatt gttaattcat ttaaattgta aactttaggg
     9901 attctctgga gttatggatg tcgttccaat atgtgtacgt tggaatgtta gtgcttatat
     9961 gggggaacag aatcctctct tgattgaaga caagttagcc attaggctgg tttgtctctt
    10021 tgtcaactgt agtgggttga tataatagta ttagtgagtg ggataaaagt ttcatttagt
    10081 ttattcagta caaatttaac gggtca
//
LOCUS       DAVDWT010000055        10036 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVDWT010000055 DAVDWT010000000
VERSION     DAVDWT010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10036
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.055"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(103..1806)
                     /gene="ilvD"
                     /locus_tag="VUW50_001939"
     CDS             complement(103..1806)
                     /gene="ilvD"
                     /locus_tag="VUW50_001939"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET7983769.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKREDGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(2039..2971)
                     /locus_tag="VUW50_001940"
     CDS             complement(2039..2971)
                     /locus_tag="VUW50_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HET7983770.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(2968..3825)
                     /locus_tag="VUW50_001941"
     CDS             complement(2968..3825)
                     /locus_tag="VUW50_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7983771.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(3829..5175)
                     /locus_tag="VUW50_001942"
     CDS             complement(3829..5175)
                     /locus_tag="VUW50_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET7983772.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMVIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSATEE"
     gene            complement(5188..5472)
                     /locus_tag="VUW50_001943"
     CDS             complement(5188..5472)
                     /locus_tag="VUW50_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET7983773.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(5476..7506)
                     /locus_tag="VUW50_001944"
     CDS             complement(5476..7506)
                     /locus_tag="VUW50_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET7983774.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NSHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNEHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            7698..8705
                     /locus_tag="VUW50_001945"
     CDS             7698..8705
                     /locus_tag="VUW50_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET7983775.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            8708..8908
                     /locus_tag="VUW50_001946"
     CDS             8708..8908
                     /locus_tag="VUW50_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET7983776.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            9060..9242
                     /gene="rpmF"
                     /locus_tag="VUW50_001947"
     CDS             9060..9242
                     /gene="rpmF"
                     /locus_tag="VUW50_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET7983777.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKITKAASAE"
     gene            9258..9407
                     /gene="rpmG"
                     /locus_tag="VUW50_001948"
     CDS             9258..9407
                     /gene="rpmG"
                     /locus_tag="VUW50_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET7983778.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(9899..>10036)
                     /locus_tag="VUW50_001949"
     CDS             complement(9899..>10036)
                     /locus_tag="VUW50_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET7983779.1"
                     /translation="ESGSMATGWVTVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWV
                     G"
BASE COUNT         3252 a         2133 c         1625 g         3026 t
ORIGIN      
        1 caaaacactg ttttgaggtt gtggatagaa ctgacgaagt cagtaaccat acctacggca
       61 aggcgacgtt gacgtgattt gaagagattt tcgagtatga gtttattttt tacctgactt
      121 gtccatattc cagaagtctg tcacggctcc gcgtgaagca gatgatacga tgtgggcata
      181 tttaccgagg acaccacggc tgtaaagtgg tggcaaggtt gtttctgcct tgcgtttttc
      241 aagttcttct tcggatacgg ccatagaaat ttctttggta tcttggtcaa ccgtaacgat
      301 atcgccggta cggagatagg caattggtcc accatcctga gcttcaggag cgatatgtcc
      361 aacaaccaga ccataagtac caccagagaa acgtccgtcc gtcaagaggg ccaccttatc
      421 tccctgacct ttaccaacaa tcattgaaga aagtgatagc atctcaggca taccaggacc
      481 acctttaggt ccaacaaaac gaacaacgac tacatcgcca tcaacgattt catctgtcag
      541 aacggcctga atcgcatctt cttctgagtc aaagacctta gctggcccaa cgtgacgacg
      601 cactttaaca cctgatacct tggcaactgc accgtcagga gcaaggttcc cgttcaagat
      661 gataagcgga ccatcctcac gttttggatt ttcaagtggc atgataactt tttggcctgg
      721 agtcaagtct gcaaagtcag ccaagttttc agctacagtc ttaccagtac atgtgatgcg
      781 atctccgtga aggaaaccat ttgccaacaa atacttcata accgcaggga caccaccgac
      841 ttcgtagagg tcttggaaga catactgacc agatggtttc aagtcggcca agtgaggcac
      901 acgttcttga atcgtattga agtcctcaag tgacaagtca acatttgcgg catgggcaat
      961 ggcgagcaag tgaagagtgg cgtttgtaga accaccgaga gccatcgtta cagtgatagc
     1021 atcttcaaag gcttcacgag tcaagatatc tgatggtttg agaccaagtt ccaacatctt
     1081 aacaacagca cgtcctgctg cttcgatatc ttctttctta tcagctgatt cagctgggtg
     1141 agaggatgac cctggcaaac tcatccctag aacttcgata gcagttgcca tggtattagc
     1201 agtatacata ccaccacaac caccagggcc agggcaggca ttacattcaa gacgtttcac
     1261 gtcctcagct gtcatgtcac cgtggttcca ttttccgata ccttcaaaga cagaaaccaa
     1321 gtcgatatct ttaccatcaa gatttcccgg tgcaatagtt ccaccatagg cgaaaatagc
     1381 tgggatatcc atattagcaa tagcaatcat agatccaggc atgttcttgt cacagccacc
     1441 gatagcgacg aaggcatcca cgttgtgacc actcatagcc gcctcgatgg agtccgcgat
     1501 gatgtcacga gatgttagag agaaacgcat accaggcgtt cccatagcga tcccgtccgc
     1561 tacggtaatg gttccaaact gtacaggcca agcgcctgca gatttgacac cttctttagc
     1621 cagtttcccg aaatcatgca agtgaatgtt acatggtgta ttttccgccc aagtcgaaat
     1681 cactcccaca atcgatgttt caaagtcctt atctgtcata ccagtcgcac gaagcatagc
     1741 acggttaggt gatttaacca tgctgtcata aatgctactg cggtgacgtt tatctaattc
     1801 agtcatctta tccctcccat ttcagttttt actattatag cacaattttc gcatgaagaa
     1861 cagaataaaa ttcttgaatt ttcagaaaat tctatacaca tgtgaaatat ttaaaattaa
     1921 aaacaacaaa gcggattagt gcactttctg atgaccagaa tatgcttttt aatccgcttt
     1981 ctttaaataa cgtactgtaa tttttacaga aattctttca aataagtgta tttaacatct
     2041 atcttgcatt ataaatttct agaaccttct cttttatatt cgattcactc aaaccatact
     2101 cattaagaag ataatccatt ttccctactt gaccgaatct ttcttgaaca cccatccgat
     2161 gaatttttgt tattccatca tcagagaata attcacataa agcactgcca attccaccta
     2221 tctgattgtg gttttctaca gtaaatatag tttttccact taacattgtt tttatctgtt
     2281 ctggtatcgg tttgattcta aataaatcta tcacacctac tgaataacct aatttagaca
     2341 gttcatctgc aactcgaata cttggagcaa ccattatgcc agaagcaacg attacaagat
     2401 cttcaccatg ccttaactca atgtagcctt tagaaaaatc ttctccacct tgatacacag
     2461 gaactggagc ttttctaatt gttcgaatat attttagtcc ttttaagtct aatgtctggt
     2521 tcaatatttc acgaaattgg atatcatcag ttgcttcgaa aatgattgat ttaggaatta
     2581 aacgtaacaa tccaatttct tcaaatggca tatgtgttcc accattcatc tctgccgtta
     2641 ctcctgcatc tgatccaatc acagtggcat ccaattgtgc gtatccaaga gaaataaata
     2701 attgatcaaa tactcttcgt gaagcaaaag gaccaaatgt atgaagataa ggtctaaacc
     2761 cctgaataga caagcctgct gcaaggccga ccatttctgc ttccataatc ccaacattca
     2821 cataacggtc tccaaagtcc ttttcaagat tattagtagc catcgaactt gacaaatcgg
     2881 cttctaagac tactatatca gaatcacttt gattagcctc taaaaggaag tctctatata
     2941 catgccgtaa ttctttcgta cttctcatca ttctgtttcc tccaattcct gacttaatct
     3001 ttctacaact gaagttaaca tttgtttctc ctctacagta gggcgaagat gatgattgga
     3061 tttcatttct tccagctctt gaaccccttg acctttaata gtatctaata caatgcactt
     3121 aggtgatgaa ttatttgact gttttaattg gacaatccct tcataaattt ctctaatatc
     3181 tgaacccttg accctaatgg attcaaatcc aaatgctgaa aatttttcta cgaaatcacc
     3241 tggattacaa atatcctttg taaaaccatc taattgtttt ttgttatcat caacaaatac
     3301 aattaagttg gataactgtt gatgagaagc aaactgtata gcctcccaac attgtccctc
     3361 atttaactca ccatctccaa caatagcgta agtataaaag ggactctttc ttattctctg
     3421 accatatgca agtccagttg caacactaat tccttgtcct aaagagcccg ttgtcatatc
     3481 tatgcctggc gttagatttc tatcaggatg agacggtaat ttggttccat ttgtatttaa
     3541 agaatataag aattctttgt caaagaaacc attcaaatag agtgtactgt atagagctgg
     3601 tcctccgtga ccttttgata atatgaaata atctctatct cgtgctgcaa atatttctgg
     3661 agtcattggc attatttcac cataaagcac cgctaaaact tctacgatag acagacttcc
     3721 tccgtaatgt ccgaatccaa gatgattcaa tgttctaaga gtatttaatc ggatgttagt
     3781 cgcaaatttt cttaacccat cttctctatt tttacttaaa atcatccctt attcctccgt
     3841 tgcagatggc tttttaataa aggatactcc aaacataact gctagaataa gaacaagacc
     3901 aatcacaatg cctgcttgtg agccaaattg atttaacatt cctaaaataa ttcctgatag
     3961 accaaaatct gcatctgaga aagttgatcc ttggaaacca agtcctccca aaactggcat
     4021 taaaaagact ggaagaaaac tgattaaaat accttgtaaa aatgctccaa tagtggctcc
     4081 acgaacacca ccagatgcat tcccaatgac acctgcagtc gctccacaga agaaatgagg
     4141 cacaacacct ggtaagataa caaccgttcc tgaagcaatc ataattacca tacttactaa
     4201 accaccaaca aaactagaga taaatccaat tagaactgca ttgggtgcat aagtataaac
     4261 aatcggacaa tccaaagcag gttttgaatt aggtacaaga cgctctgaaa tacctttaaa
     4321 ggctggaaca atttcgccca aaataaggcg aacacctgct aaaataacaa atacccctgc
     4381 tgcaaattga cctgctaatt gtaaagcata aactagacca cttgtaccac tactgatttc
     4441 tttttctata tattctgacc ctgcaaagat agctacaata atgtaaataa ctgccatgga
     4501 taaagtaata ctaacagtac tatcacgtaa aaaagctaaa ctctttggaa atttaatgtc
     4561 ctctgttgat tttgatttgt caccgataag gctaccagta aaaccactca accaatatcc
     4621 caaagaactg aaatgaccta aagctacctt gtcatttcca gttaattgaa ccatatattt
     4681 ttgcacaaat gctggggaaa tactcataat aataccgagt gctaatcctc ctagtaagat
     4741 gagaggcaag ctagtaaagc cagcaactga taaaatgacc gcaatcatac atgccatata
     4801 tagagtgtgg tgccctgtta aaaaaatata tttaaatcga gtaaaacgag cgattaagat
     4861 attgaacacc atgcctgcaa acataatcat tgcagtagct gagccatatg ttgttaaagc
     4921 tacagctaca attgcttcat tattcggcac aacgccagat aaatgaaaag catgctcaaa
     4981 catggtacca aatggattta aagaattttg tacaattcct gcaccaccag atacaactaa
     5041 gaaaccaaca aaggtcttaa ttccaccttt aataatatca ggtaatttct tcttctgaag
     5101 aactaatcct aagattgcaa ttaaagctac taaaatagct ggtgtactaa caatatccaa
     5161 tatgaacttc atcatgacgc tagcctccta tataagtcct ttttcttcac aaagtttagt
     5221 aattaattct cgtagttcat ccatatcaat aatactattt aagatacgaa catctccaag
     5281 atgactagct gaatcagcta gatcacgacc aacaatccaa atatcagctg catttggatc
     5341 tgctccacct aaatcataat gttcaacttc tacatccgaa acattcaaat cactcaatac
     5401 agattcaata ttcatctgta ccataaaact tgaacctaat cctgaaccac aagctgtacc
     5461 aatttttaac attatctaat cctcctgttt aattatcatt ttaatgtcat catagttttt
     5521 tgatgatatt aaagtttgaa catgattttt atctcttaaa attgttgtta aatgtgacaa
     5581 agcctttaaa tgactctcat tatcaatggc tgcaatacaa atcaacaatc ttacctcttg
     5641 ttctggatta tccaataaat aaatcggttc ttccaaaact aacattgaca ttcctatttc
     5701 attcacacct tcatctggcc gagcgtgagg aattgctact cccttcccta aattaataaa
     5761 aggtccaaac tcttctactt tttgaatcat tgcctcaggg tagttctcag ttatcttatc
     5821 ttgatccaaa agcggtttag ctgctaaacg aatcgcctcc ttccatccta atttttgcga
     5881 actaacctga taggtttctt tggtaataag ttgttctagc actggtacaa tttcctttct
     5941 atcatttttt tggtaaagat aattctttaa cgccaatctt aattccaatt cttgtgtaat
     6001 aattccatat cttttgacaa tattcaggat ttgttcaatc tcaaaatctc catactctaa
     6061 attcggaaaa tcttttaaca ctagttctac tagttgtatt gcttgctctt cagtcatcat
     6121 aaccgaaact agataatttg gcttttctgt ctccaccttt atggtagaaa aaaccatatc
     6181 atagtcacta ctagctttca cctgtaaatc atcaatcttt gaggttccta taaactcaat
     6241 ttgaggaaat aatgctaata gattctcttt taacatcaat gaagaactaa caccattagg
     6301 acaaatgatt gctgctttat accatttttg aggcaaagta tctgctttct ttaaataacc
     6361 tccgaaatgg ataacaaaat atgctgtttc actatcaggt atgggattgt caatagcgtc
     6421 catcaagggc atcaaagaat ctttgactaa ttcaaataaa tcaggataat gttctttaac
     6481 atgcaataca tattcatttg aactaggtag gccgaacttt aatctatagt aagccggtat
     6541 aaggtggcgg cgaagatttt ctctcaatcc ttccctttgt ttaaaatgta acaaagaaat
     6601 atcttccatt ctacttataa tagcctctgt taattgatta aagtaaaccg gagcaacatc
     6661 tacttcacct tcaaagcaac ttgataataa aacggtgata tagcgataat catcctcaga
     6721 aaacaccgta tctataattc ccaaatcaac cactgtatcc aataaaatag tggttatatc
     6781 ttgaataaca ggagatacta atgtctctga aagacatact ctttcaacat ccctttgata
     6841 cctacacaga atgaatacta aaccgaaaag gtaaactttt aattgattaa caataggtac
     6901 tagctgtagc ttctcataat aatctttaac tacctgatca atcaaatcat aagttaatga
     6961 atacccccaa ctggataaaa cataatccaa accccaaatc cctatggagg attccagcaa
     7021 ctcactaacc atttgaaaag ctaagcggtg ctcattccac tctgaaccgt gtaaagtata
     7081 accttttgct ctactgtacc ctagctccaa atcattatct aacataatct ttcttaatga
     7141 ttgaatatca gataaggttg tattcttact tactttcaaa aagtcttggt aatgactatt
     7201 cgatataaaa tctaatcggc aaaaagtgta aagatagatt aaagctaagc gagtcgactt
     7261 tggtaaaacc aattcatccg acttaataat atctgtcaaa gactgcttcg tacgatttga
     7321 taaactatag cgaccttgct ttttatccag cactatccct ttattagcta gataaggcac
     7381 taaataatct attccttctt tgacttcctt tataggtaag ctcaccttaa cagataattc
     7441 atataatgat agctcacaat gatccatcaa agtcatcaaa ataactagtg ctctataatc
     7501 aaacatttct cttcctttct aactacaatt atagtgtaaa aattataaga ataaaagagt
     7561 atttgagcac aatataatct caccttttaa aagaaattat tgggctgaaa gtttttagtg
     7621 attatttttc aactttctat tgactttata aattaaagtg atatcattta gatatcaaat
     7681 aacaggaggt cattcttatg actgaaaaac taatcaattc aaaaccaaat ggtgtattcg
     7741 cattgattct cattgagttg acaatcgtac ttggtatctt tatatttata atgggggttg
     7801 gttcggaaaa catttttgga attattatcg gacctttact aatcgtaatt gcaggtctag
     7861 ctcatgctgg tttaaaagtt gtcaaacctc aagaagctct ggttctgaca ctctttggta
     7921 actatacagg taccatcaaa gaacctggct tttactttgt caatcccttc agcgtagcag
     7981 tcaaccctgc aaaccacact cgacttggac aaagtggtga tgttagcaca aaatctcctt
     8041 ttttaggagc taaatcatca aatgacaatg atgtaaatct tgaaattggc aagaaacaga
     8101 tttccctcaa agtcatgacc ttgagcaatt ctcgtcaaaa aatcaatgat tgcttaggaa
     8161 accctgtaga aatcggtatc gcggtaactt ggagagttgt cgataccgct aaggcagtct
     8221 tcaacgttga taactacaaa gaatatcttt cattgcaatg tgatagcgcc ctccgtaata
     8281 ttgtccgcat ctatccttac gatgtgtctc ctaatgtgga tactacgggt gatgggcaag
     8341 cagatgaagg tagtctccgt ggctctagcg aaattgttgc taaccgtatt cgtgaagaaa
     8401 tccaaagtcg tgttgaggat gctggcttgg aaatccttga agcacgtatc acttacctag
     8461 cttatgctcc agaaattgct gccgttatgc ttcaacgcca acaagcatct gccattattg
     8521 atgcacggaa gatgattgta gatggtgctg taggaatggt tgaaatggca ctagaacggc
     8581 tcaatgaagg ggaattggta gaacttgacg aagaacgaaa agctgccatg gtttcaaatc
     8641 tccttgtcgt tctttgtggc aatcatgatg cacaaccaat tgtcaacaca ggaagccttt
     8701 actaaagatg gctgaagcta aaaaaaagca gatccccctt cgactctcaa caaagttata
     8761 cgctgcactc gcatcatggg cagaagacga ctttcgttca gtcaatgggc aaatcgaata
     8821 tctcctcaca gagtgtgtca agcaacggaa gaaagacgga aaatacgtat cagaaaccat
     8881 tgacgaacca tttgagattg atatttaaca ccttctcctg tctgccgtgt cggataggag
     8941 aattttttca tctttttttg tcaagtaact ttactttaca aaaaaaatgt gttatcctag
     9001 tatggttgat gaaaatcagt agattgaatc gaatataaat acctaaagga gaaatcaaaa
     9061 tggcagtacc tgcacgtcgc acttcaaaag cgaagaaaaa caaacgtcgt acacactaca
     9121 aagtaacagc tccatctgta aactttgacg aaactactgg agattactca cgttctcacc
     9181 gtgtatcact taaaggatac tacaaaggac gtaaaatcac taaagctgca tcagctgaat
     9241 aatagaaggg agataccatg cgcgtaaata ttacacttga acacaaagaa tctggtgaac
     9301 gcttgtacct tacttctaaa aacaaacgta acactccaga ccgccttcaa ttgaagaaat
     9361 actcaccaaa acttcgcaaa cacgttgtgt ttacagaggt taaataagca ttcaatacga
     9421 atcaatacag aagaaaaacg cttgatcagc gttttttctt tttgttaatt tcactacatt
     9481 gtaagggaaa tcattcgaat gactacattt ttcactacct tttttgaaat aagaatacgg
     9541 attcggacaa ttaaaagcaa gaggaactca aatgtaagtt ggggttcctt ttttgtatat
     9601 ttttcaaata acgcctctac actatttgta gcaaattcac caactacagt tgtatcttag
     9661 ttaaaataag ttagaatatg taagtgagta ccagatatac caagacatcg tcaccatcta
     9721 aggtatattc aaaatacaaa agttgaccaa ctagatttct gaatatcctt atatatccat
     9781 tcttaaaatt ggtttaaata gcgtagtctt ttaaactagt tttgagaatc caaaaaatct
     9841 tcctacatat gtaagaagat tttttagttc agaatgatta gatttagcta atggatacct
     9901 atcctaccca ctcaccgtta gcattgactc gatagccatc gggtgtagtc gtgtttacta
     9961 ataaatctcc tgaattatag gtatagtacc atttaccaga aactgtaacc caaccagtag
    10021 ccattgaacc tgattc
//
LOCUS       DAVDWT010000056         9773 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVDWT010000056 DAVDWT010000000
VERSION     DAVDWT010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9773
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.056"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..89
                     /locus_tag="VUW50_001950"
     CDS             <1..89
                     /locus_tag="VUW50_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET7983780.1"
                     /translation="STTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            126..602
                     /locus_tag="VUW50_001951"
     CDS             126..602
                     /locus_tag="VUW50_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HET7983781.1"
                     /translation="MPYFIGGHPGFNCPLLDDEVYEDYYLESEKEETCSVPRPFPETG
                     MLDFQDRSPWLEGQKEIDLSYDLFSKDAVTLDELQSRTIALRSLKHDKGLKMHFAEFP
                     NLIIWSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            759..1184
                     /gene="lacA"
                     /locus_tag="VUW50_001952"
     CDS             759..1184
                     /gene="lacA"
                     /locus_tag="VUW50_001952"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HET7983782.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            1215..1730
                     /gene="lacB"
                     /locus_tag="VUW50_001953"
     CDS             1215..1730
                     /gene="lacB"
                     /locus_tag="VUW50_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HET7983783.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            1741..2670
                     /locus_tag="VUW50_001954"
     CDS             1741..2670
                     /locus_tag="VUW50_001954"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HET7983784.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            2672..3652
                     /gene="lacD"
                     /locus_tag="VUW50_001955"
     CDS             2672..3652
                     /gene="lacD"
                     /locus_tag="VUW50_001955"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HET7983785.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3886..4722
                     /locus_tag="VUW50_001956"
     CDS             3886..4722
                     /locus_tag="VUW50_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET7983786.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLITENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4758..5075
                     /locus_tag="VUW50_001957"
     CDS             4758..5075
                     /locus_tag="VUW50_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET7983787.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEVGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            5075..6766
                     /locus_tag="VUW50_001958"
     CDS             5075..6766
                     /locus_tag="VUW50_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET7983788.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGASVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFVPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VEAAQNTITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            6975..8381
                     /gene="lacG"
                     /locus_tag="VUW50_001959"
     CDS             6975..8381
                     /gene="lacG"
                     /locus_tag="VUW50_001959"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET7983789.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            8471..8611
                     /locus_tag="VUW50_001960"
     CDS             8471..8611
                     /locus_tag="VUW50_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HET7983790.1"
                     /translation="MNFEKCSQIPCLTSEELKSLGKWYVSTGKEWDTISFDSDFMPFQ
                     QS"
     gene            complement(8890..9651)
                     /locus_tag="VUW50_001961"
     CDS             complement(8890..9651)
                     /locus_tag="VUW50_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET7983791.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNISNLDTIVSDSKLSDSILFKLSKHIKVIKP"
BASE COUNT         3073 a         1749 c         1972 g         2979 t
ORIGIN      
        1 tttcaacaac ctatcgaaat catcgtaaac gcttcggatt acgaatgaat ttgattgctg
       61 gtattatcaa tcatgaacta ggattctagt tttgcaggaa gtctaataaa gaaactagca
      121 aggtcatgcc ttactttatt ggtggacatc caggatttaa ttgtcctcta cttgatgatg
      181 aagtctatga agattattat ctagagtctg agaaagaaga gacttgctct gttccacgtc
      241 ctttcccaga aacaggtatg ttggatttcc aagatagaag tccatggcta gagggacaaa
      301 aagaaataga tcttagttat gatcttttca gcaaagatgc agtgactttg gatgaattgc
      361 aatctcgaac aattgccctt cgttctctta aacatgataa gggattgaaa atgcattttg
      421 cagaatttcc aaacctcatc atctggtcaa ctttgaacaa gggacctttc attacctttg
      481 aaccatggtc tggcttgtca acattccttg aagaaggaga tcatttagaa gataagaaga
      541 atgtttgtct cttagaagcc aatcaggttg aagaattagg gtttgagata gaagttttat
      601 aaaaaagtaa aaaataaata acaaaaacaa acacaaactg ttgacttctt caaacaatag
      661 tagtatatta tgtttgtgag gactaattga ctgtttttta aagacagcca atacactgct
      721 ggaatccagc atagaaaaaa taaaaaggag tatacaatat gtctattgtt atcggtgcag
      781 atgctgcagg tttgagattg aaagaagttg tgaaagactt cttagaaaaa gaaaacttcc
      841 accttgtgga tgttacagct gaaggtcaag actttgttga tgtgactctc gctgttgctg
      901 cagaagtaaa caaagaagaa caaaaccttg gtatcgtgat tgatgcttat ggagctggtc
      961 catttatggt tgcaactaag atcaaaggaa tggttgctgc agaagtttct gatgaacgtt
     1021 cagcttatat gactcgtggc cacaacaact cacgtatgat cactatgggt gcacaacttg
     1081 ttggtgatga attggctaaa aacatcgcta aaggatttgt taacggtaaa tacgatggtg
     1141 gacgtcatca aatccgggtc gacatgttga acaaaatggg ctaattagat tacagtaaga
     1201 aaggtaagtt aaaaatgaga attgcaattg gatgcgacca catcgtaaca gatgaaaaaa
     1261 tggcggtttc agaatttttg aaatcaaaag gatatgaagt cattgacttt ggtacatatg
     1321 accatacacg gactcactac ccaatctttg gtaaaaaagt aggggaagct gtaactagcg
     1381 gccaagctga tcttggggta tgtatctgtg gtactggtgt tggtatcaac aacgctgtaa
     1441 ataaagttcc aggtgttcgt tctgccttgg ttcgtgatat gacaacagcc ctttatgcta
     1501 aagaacaatt gaacgctaac gttatcggct ttggtggtaa gattactggt gaattgctta
     1561 tgtgtgatat catcgaagct ttcattcatg ctgaatacaa accatctgaa gaaaacaaga
     1621 aattgattgc gaaaattgaa caccttgaaa gtcacaatgc tcaacaaaca gacgcaaact
     1681 tctttacaga attccttgaa aaatgggatc gcggagaata ccacgactaa gaggtgacct
     1741 atgattttaa cagtcacaat gaacccatcc attgatattt cctatccctt ggatgagttg
     1801 aagattgata ctgtcaatcg tgtggtggat gtaaccaaaa cggctggtgg taagggactc
     1861 aatgttaccc gagtactttc agaatttggc gattctgttc ttgctactgg tttagtgggt
     1921 ggcaaacttg gtgagttttt ggttgaacat atcgataatc aagtaaagaa agatttcttc
     1981 tcaattcagg gagaaactcg taactgtatc gctattctcc acggagacaa ccaaacagaa
     2041 gttcttgaaa aaggtcctga agtattggaa caggaaggtc aagacttttt ggaacatttc
     2101 aaaaaactct tggagtcagt tgaagtagtg gccatctcag gtagtctgcc agctggcctt
     2161 ccagttgatt actatgcgag cttggtagaa cttgctaatc aagctggcaa gcctgtagtc
     2221 ttggactgct caggtgcagc acttcaggct gttcttgaat caccccataa accaacagtc
     2281 atcaaaccaa ataatgaaga attgtctcag cttcttggaa gagaagtttc tgaggatttg
     2341 gatgaattaa aagaagtact tcaagaacct ttgtttgcag ggattgaatg gattatcgtt
     2401 tcacttggtg ccaacggtac ttttgccaaa catggtgaca ctttctacaa ggtagatatt
     2461 cctagaattc aggtggtaaa tcctgttgga tctggagact ctactgtggc aggaatttct
     2521 tcaggacttc ttcacaaaga atcggatgca gaattactca tcaaggcaaa tgtccttggt
     2581 atgctcaatg ctcaagaaaa aatgactggt catgtcaaca tggccaacta tcaagctcta
     2641 tatgatcaat taatagtaaa agaggtataa aatggcttta acagaacaaa aacgtgtacg
     2701 cttagaaaaa ctttctgatg aaaatggtat catctcagct cttgcatttg accaacgtgg
     2761 tgctttgaaa cgcctcatgg ctcaacacca aacagaagaa ccaactgtgg cccaaatgga
     2821 agaacttaaa gtcttggtag cagatgaatt gactaaatac gcttcatcta tgcttcttga
     2881 ccctgagtat ggacttccag caactaaagc tcttgatgaa aaagctggtc ttctccttgc
     2941 ttatgaaaaa acaggttatg acacaacaag cacaaaacgc ttgccagact gcttggatgt
     3001 ttggtctgca aaacgtatta aagaagaagg tgcagatgca gttaaattct tgctttacta
     3061 tgatgtagat agctcagacg aactcaatca agaaaaacaa gcctacatcg aacgcatcgg
     3121 ttctgagtgt gtggctgaag atatcccatt cttccttgaa atccttgctt acgatgaaaa
     3181 aattgcggat gcaggttctg tagaatacgc gaaagtaaaa ccacacaaag ttatcggcgc
     3241 tatgaaagtc ttttcagacc cacgctttaa cattgatgtc ttgaaagttg aagttcctgt
     3301 taacattaaa tatgttgaag gcttcgctga aggtgaagta gtttatacac gtgaagaagc
     3361 agcagccttc ttcaaagcgc aagatgaagc aacgaacttg ccatacatct acttgagtgc
     3421 tggtgtatca gctaaactct tccaagatac tcttgtattt gctcatgaat caggtgcaaa
     3481 ctttaacgga gttctttgtg gccgtgctac atgggcagga tcagttgaag cttacatcaa
     3541 agatggtgaa gcagcagctc gcgaatggct tcgcacaact ggatttgaaa acattgatga
     3601 gctcaataaa gttcttcaaa caacagcgac ttcatggaaa gaacgtgtgt aagaaagtcc
     3661 tcctagttta ggaacatgaa tctaaaaaaa tttaaaaaaa gttgtatgta aaggtttaca
     3721 aaataactta cttgtgctat acttaaatca caagttaata caaggtgagt gttactaagt
     3781 aatattaggc atgatcacag gtgaattaga aatcagctga ttttctagtt catttgtggt
     3841 cattttttgt actcatatac ctttaagata taaaaggagg ttgacatgta tcgaattcta
     3901 aatccaatga atcacaatgt ctcgcttgtc agaaatgata agggagaaga ggtgattgta
     3961 attggtaagg ggattgcatt cggaaagaag aagggggatt tgattactga aaatcaggtt
     4021 gagaaaatct ttcggatgaa gaccgaagag tccagagaaa actttatggc tcttctcaaa
     4081 gatgttccgc ttgattttat cacagtgacc tatgaaatca ttgataagct atcaaagaaa
     4141 tatcattatc cgattcaaga gtatctctat gtaaccttga cagatcatat ttactgttct
     4201 tatcaagctc taactcaagg aaggtacaag gatagtaatc tgccagatat ttccgctaag
     4261 tatcctgtcg cttttcaaat cgcaaatgaa gcttttgaaa tttaccggca gaagctagca
     4321 gatcattttc ctgaggacga aattattcgg attgcttatc atttcattaa tgctgaaggt
     4381 gaaaatgaag tggaacttgt ggagtcgatt gataagagga aagaaattct caggaatgtt
     4441 gaagaagttt taacggacta tgcaattcaa cgaactaaaa agaataacca tttctatgat
     4501 cgctttatga tccatttgaa ttatttcttg gattatttag acagatctag agatgataac
     4561 caatcacttc tggatatgga agatcatatt aaacaatcct atccaaaagc cttcgagatt
     4621 ggttccaaga tctatgatgt gattacgcaa catacgggtc ttgatttgta taaaagtgaa
     4681 cgagtttatc tagttctaca tatccaacgt ttattgtcat aaaaatttat ttaaaactat
     4741 ataaggagaa ttctatcatg aatagagaag aagtaacatt gttaggtttt gaaatcgtag
     4801 cctatgctgg cgatgctcgt tcaaaactat tggaagcctt gaaggctgct gaagttggtg
     4861 attttgaaaa agcggacgct ctggtagagg aagctggttc ttgcattgct gaagctcatc
     4921 atgcccaaac aagtctattg actaaggaag cttctggtga ggacttagcg tatagtgtga
     4981 ccatgatgca tggccaagac cacttgatga caactatctt gttaaaggat ttgatgcacc
     5041 atttgatcga actctacaag agaggagtta aataatgaac aaactaattg catttatcga
     5101 gaaaggaaag cctttctttg agaaactatc tcgtaatatc tatcttcgtg ctattcgtga
     5161 tggtttcatt gcaggtatgc ctgttattct cttctcaagt atctttatct tgattgcctt
     5221 tgtaccaaac tcatggggct ttaaatggtc tgatgaagtt gtagcctttc tgatgaaacc
     5281 ttatagctat tctatgggta ttctggctct cttggtagct ggtacaacag ctaagtcatt
     5341 gactgactca gtaaaccgga gcatggaaaa aaccaatcaa atcaactata tgtcaacatt
     5401 gttggcagca attgttggtt tgttgatgtt ggcagctgat cctatcgaaa gtggtctagc
     5461 tactggattc ttggggacaa aaggtttgct ttcagccttc cttgctgcct tcgttactgt
     5521 agccatctat aaggtttgtg ttaagaacaa cgtcactatt cgtatgcctg acgaagttcc
     5581 accaaatatc tcacaagtct ttaaagatgt gattccattc actctctcag ttgtttcact
     5641 ttatgctctt gatttactag ctcgtcattt tgttggtgca agcgtagctg aatcaatcgg
     5701 taaattcttt gcaccactct tctctgcagc tgatggctat cttggtatta ccattatctt
     5761 tggtgccttt gccttcttct ggtttgtggg tattcatggt ccatctattg ttgaacctgc
     5821 tatcgcagca attacctatg caaatgctga agtcaacttg aaccttctcc aacaaggtat
     5881 gcacgctgac aagattctta cttctggtac acaaatgttt atcgttacca tgggtggtac
     5941 tggtgcgaca ctggttgttc cattcatgtt catgtggtta acaaaatcaa aacgtaaccg
     6001 tgcaatcgga cgtgcttcag tagttcctac cttcttcggt gtaaatgagc caatcttgtt
     6061 tggtgcacct cttgttttga atccaatctt cttcattcca tttatctttg ttccaattgc
     6121 aaacgtatgg attttcaaat tctttattga aactcttgga atgaactcat tcactgctaa
     6181 tctaccatgg acaactccag ctccactagg tctagttctt ggaactaact tccaagtgct
     6241 atcattcatt cttgctgccc ttctaatcgt ggttgacgtt gtcatttact atccattcct
     6301 taaggtctat gatgaacaaa ttcttgaaga agaacgttca ggtaagtcta atgatgaatt
     6361 gaaagaaaaa gttgctgcaa acttcaacac tgcaaaagcg gatgctattc ttgaaaaagc
     6421 gggtgtagaa gcagcacaaa atacaatcac taaagaaaca aatgtcctcg ttctctgtgc
     6481 aggtggagga acaagtggac tccttgcaaa tgctttgaat aaggcagcag cagaatacaa
     6541 tgtccctgtg aaagcagcag caggcggcta tggtgctcac cgtgaaatgt taccagagtt
     6601 tgatcttgtt atccttgccc ctcaagttgc ttcaaacttt gaagatatga aagcagaaac
     6661 agataagctc ggtattaaac tagcgaaaac agaaggcgct caatacatca aattaactcg
     6721 tgatggaaaa ggtgctcttg cattcgtaca agcgcaattc gattaaggct agagactctg
     6781 aaatagtctc ctatcgttac ggaaatcgct atggcgaatt tcctattatt aattcgtcgg
     6841 taaaaagata tcgtttttac ctcctcatgt cacaattcgg tgacttggta caagaagtga
     6901 gatggagaag gatggctcac tgactcctct cctctcactt ttactttatt taaatcaaga
     6961 aataggtgaa aaaaatgaca aaaacacttc caaaagactt tatttttggt ggcgcaacag
     7021 ctgcctatca agcagaaggt gctacacata ctgatggaaa aggaccagtt gcttgggata
     7081 aatatcttga ggataactac tggtacactg ccgaaccagc tagtgatttt tacaatcgat
     7141 atccagttga cctcaagcta gcagaagagt atggtgtcaa tggtattcga atttctattg
     7201 cttggtcacg tattttcccg actggttacg gccaagtaaa tgctaaaggt gttgagtttt
     7261 atcataattt atttgcagag tgtcacaaac gtcatgttga gccttttgta actcttcatc
     7321 actttgacac gccagaagct ctccactcaa atggagactt cttaaaccgt gaaaatatcg
     7381 aacattttgt agactacgct gccttctgtt ttgaagaatt tccagaagta aactattgga
     7441 caaccttcaa tgaaattgga ccaatcggtg atggtcaata tttggttggg aaattccctc
     7501 caggtatcca gtacgacctt gccaaagtct ttcaatcaca ccacaatatg atggtgtctc
     7561 atgcacgcgc ggtaaaattg tacaaagaga aaggctataa aggggaaatt ggtgttgttc
     7621 acgccctgcc tactaaatat cctctagatc ctgaaaatcc agcagatgtt cgtgcagctg
     7681 agttggaaga tatcatccac aataaattca tcttagacgc aacttatcta ggtcgctatt
     7741 cagctgaaac catggaaggt gtcaaccata tcttatcagt caatggtggt agtttggatc
     7801 ttcgtgaaga agattttaca gcattagaag ctgcaaaaga cttgaatgat ttcctaggaa
     7861 tcaactacta tatgagtgac tggatggaag cctttgatgg agaaactgaa attatccata
     7921 atggtaaagg tgaaaaagga agctctaagt atcaaatcaa aggtattggt cgtcgtgtag
     7981 ctcctgacta tgtatcacgc acggattggg attggattat ctaccctcaa ggtttgtatg
     8041 accaaatcat gcgtgtgaag aaagattatc ctaactacaa gaaaatttac atcactgaaa
     8101 atggtctcgg ctataaagat gagttcgttg ataacactgt ttacgatgat ggtcgtattg
     8161 attacgtgaa acaacacttg gagatcttgt ctgatgcgat tgcagatgga gctaatgtaa
     8221 aaggttactt catttggtca ttaatggatg tcttctcatg gtcaaacggt tatgagaaac
     8281 gttatggtct cttctacgta gattttgaaa ctcaagaacg ttatcctaag aaatcagctc
     8341 actggtacaa gaaagtagcg gaaactcaga ttatagacta gtagaattag tcattagata
     8401 tagaatttta gtgagtcaaa aagatgttca aagattttat ccaatctatt tatgaaaaaa
     8461 agtttatatt ataaatttcg aaaaatgctc tcaaataccg tgtttgacga gtgaagaatt
     8521 gaaaagtctt ggaaaatggt atgtctcgac tggtaaagaa tgggatacta tctcctttga
     8581 ttcagatttt atgccgtttc aacaatcgta accaatttct caaaaaagtt aaatcttata
     8641 tttagtactc tgtaaaactc ttatctaatc acgttgctta tactcaatga aaatcaaaga
     8701 gcaactttaa actaggaagc gagtcgcaga tttctcaatg catagctttg agaaattggg
     8761 caaaaagtct ttgatataga aaaaagcata gtatcaggtg ttgaaacacc tgatactatg
     8821 ctttttattg tgggaagatt tacttttttt cttctgaaat tgagttgtta cccaggctct
     8881 ttcagtttat taaggcttga tgactttaat gtgtttagat agcttaaaaa ggattgaatc
     8941 acttagttta gaatctgaaa caatagtatc aagatttgat atattataaa aagtataaaa
     9001 atcaaactta ttgaacttac tatgatctgc gagtaaatat tttttattag aattatttaa
     9061 agcgatgcgt tgagcctctc cctcttcctc gctaaaagta gctagagctc cgttttgaat
     9121 accattacag ctaacgaaag ctttagaaaa ttggagatta gagagatttt gtagggtcaa
     9181 tgtgccaaca aaagcacctg taatatcgcg ataatttcca cctattaaaa tcaaatctgt
     9241 taattttcgt tcgcttaaaa tcagaaaaac aggtagactg ttggttacga cgcggatatt
     9301 gtcaataggc aactcacgcg caaaaaactc taatgttgtt cctggtccaa tgaaaatagt
     9361 ttctctttct tctactagac tgcctgcaaa atgggctatt tcttgttttt ctgccgtttg
     9421 gagggcttgt ttttcaatat ttgatcgctc attagtcaaa agggagttgg ttcgaagttt
     9481 ttcagctcca ccatgcacac gaatcagcaa atctttatca gctaattcct gtaaatagcg
     9541 ccttgcagtc atatctgaaa cggctatttc gtccataatc tgtttaaccg ttatggttcc
     9601 tttgctattt actatctcta aaattttggt taatttttct tgtttgagca tattatcacc
     9661 tcgtttccta ctactatctt accataaata aactcatcat tcaaatacaa aaacaacaaa
     9721 atgaaacaaa aacaaaaata tcgaagtttg ttttcaaaac tttcgatatt ttt
//
LOCUS       DAVDWT010000057         9573 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVDWT010000057 DAVDWT010000000
VERSION     DAVDWT010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9573
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.057"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            247..507
                     /locus_tag="VUW50_001962"
     CDS             247..507
                     /locus_tag="VUW50_001962"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7983792.1"
                     /translation="MSGGETKRVALAKIILKNPPLIFADELTASLDSTTSQGIMKLLL
                     GMKNKNRLIIIATHNPDIWNQADEVINLNQLLQSIYIFYILK"
     gene            complement(611..1081)
                     /locus_tag="VUW50_001963"
     CDS             complement(611..1081)
                     /locus_tag="VUW50_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET7983793.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQTLLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(1098..3371)
                     /locus_tag="VUW50_001964"
     CDS             complement(1098..3371)
                     /locus_tag="VUW50_001964"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HET7983794.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            3718..6819
                     /locus_tag="VUW50_001965"
     CDS             3718..6819
                     /locus_tag="VUW50_001965"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET7983795.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGQSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIKEAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            6902..7909
                     /gene="pfkA"
                     /locus_tag="VUW50_001966"
     CDS             6902..7909
                     /gene="pfkA"
                     /locus_tag="VUW50_001966"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET7983796.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGETSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            7968..9473
                     /gene="pyk"
                     /locus_tag="VUW50_001967"
     CDS             7968..9473
                     /gene="pyk"
                     /locus_tag="VUW50_001967"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET7983797.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATNSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
BASE COUNT         2961 a         1658 c         2101 g         2853 t
ORIGIN      
        1 aaataggctc cataatatct atagtggatt tacccactac aaatattata gaaccctttt
       61 ttattgacag tatcaggata tctttttcaa atttttggtt tgttagataa tgaaaatgtt
      121 tctatcaatc tagatttagg attaataaat cgtaaatgta attataaaga aaaagataag
      181 ttaaaaaaag atgtattagc aagagttggt ctaactcata ttcatctata acaaaagata
      241 tatgaattgt ctggcggtga aacaaaacgt gttgctttag cgaaaatcat tcttaaaaat
      301 ccaccattaa tattcgcgga tgagttgact gcatcactcg attcgacgac ttcacaagga
      361 attatgaaat tgttattagg tatgaaaaat aaaaatcgtc taatcatcat tgcaactcat
      421 aatcccgata tttggaatca agcagatgaa gttataaatt taaatcaatt attacagtca
      481 atatatatct tctatatttt aaagtaattt tatctgaatc cgttaagaga ctattgtccc
      541 aggctctttc tgatgttaaa gactttctac caggtttttt aaaagcataa ttgttagttg
      601 tagtcattta ttattcttca aagaaaaatg gtggggcgaa ttttttcagt tcttcaaagc
      661 acttttgagc agtatctgca tcttcacaga tgataagaca gacatcatta ccacaaaggg
      721 tagcgatagc gtcagggaag ctcaaagtat caatgataga accaaaggat tgagccagtc
      781 caggaagggt ttttagtagg acttggtgtt gaactgggcg catccagaca agggcgtctt
      841 ccatgtagag ttcgagacgt ttttcccatt ttgagatgga accattgtta agaacataat
      901 aagcgctatc ttcttcgcgg acttttgata ggttcatatt tttgatgtcg cgtgagaggg
      961 ttgcctgagt tacttgaatg tcgttctcag caagaagggt ttgcaactca gcctgtgtat
     1021 gaatcttgtt ttttgtgata agagcgcgta taagttggtg gcggtgttct gatttattca
     1081 taataatgta actcctttta gcaaggtaag gtaagcatgg actgagcgag gtcgacagtc
     1141 aagtggtagt ctgtattgtc acggatggtg atttcaaagt cagtagtata gaggactaaa
     1201 cggagagtgt ctccttcttt tagcttgtaa atagttggct gcagttcaaa ttgaacgtcc
     1261 atccattcat ctgcagtaat atcctctact aacagtaaat catttctatt ttgtaaatta
     1321 aggtaacctt ttgtcacgac tctttgtgcc tctggtctaa atggcaattc acagagattt
     1381 tccaacatgt gatagcgacc gttgtcaatg gttctagcac ttaaaatagc tggataaggt
     1441 tgtaggtatt tcttttgccc aaattctagc agttgggcag ataagagccc cttgtttgta
     1501 ctggatttga tacgaagatt gagctgagcg cgaccgttta ggtggagatc tttagtcaca
     1561 ggaaggttaa tagtaatctg attggctttc ccttgataga gctctgtatt gaaggtttgg
     1621 tatgtcttac catagcgctc aaaatcctta tctgggtact ggttttgaat agcttgctct
     1681 tcttgaccaa gtgagaaggt ttcacagttt tcttgcccac cgaagttatc aagtgataac
     1741 caagtctgtg gagctgtatt gtcctgccag ataacagtag gaagttgaaa gtctgtttcc
     1801 tgtcctagta atttcttggt caataaggca tttatggact cacggaagtc aattgattgc
     1861 caattgttca tgtaaacatg ggcaccatta tggaaaaaga gatgcttgtg tatatgagta
     1921 ggaagagcat ggaacatctg gtaaacatga agtggtttga cattccaatc ctgagaacca
     1981 tgagtaaaga caacctctgc ctttacttta tgggcattga gcagataatt gcggtcatgc
     2041 caaaactgat tgtagtcccc agtttttcgg tctagctgag ctttcacttt ttctaagtca
     2101 gcttggtgag cttcattgcc acggatatag tcgccagcta agagattacg agaataggtt
     2161 aactcagcaa gggagtcaaa gtcctcacct ggataaccac ctgggctagt caccagaccg
     2221 ttttcacggt agtagttgta ccatgatgaa attcctgcct cggcaatgat aacttctaaa
     2281 ccatcgactc ctgtagtcgc aagaccattg gacatggtac ctagatagga aagtcctgtt
     2341 gtagcaactt ttccgtttga ccaatcagcc ttgacttgac gctggcgcgt gtgatcagta
     2401 aaggcacggc aacgaccgtt aagccaatcg atgacatttt tataagcctc gatttgctgg
     2461 tagtctccat tagtcatgaa acctgtcgag tctttggtac caacacctga gacatagaga
     2521 ttggcaaagc ctctcggaag gaagtagtcg tttagtgtat agctagagtt gatgtgagtt
     2581 agcttttcct cagcctctgc tataagctca gctttacctt ggggttggac gagatttagt
     2641 tgaggtttct ctagctcaat cttgtgagga agcttaacct caagctcgcc ctccatcttg
     2701 tagagagcct tgtcactagc cttgtcattg gttccctgat gataagggct ggctgtcatg
     2761 atggcaggga tttttccatc aaaacgaggg cgaataatgc taacctttac taggtctgat
     2821 agcccttttt ggtcagtatc gacacgagac tcaacgtaaa cgacttcacg aatgacatcc
     2881 tggttagaaa aagtagccaa actcttgccg ttaaagtagt ggtagtcatt atcctccgga
     2941 ataagaccat cactaacaag ttggtcgata agagtatttc cttttttggt gcgagtattg
     3001 agtaactgat agagattttc aatcaagtca ccatatataa tgggaaatcc agtttcttta
     3061 cgaaaaacgt cactatcttc gaagtcaacc aaataagaaa agcctaaaag ttgaaaagca
     3121 acagtataaa aaatatctgc tgtcagttca tcttctgatt gaaaaaatgt cagcaggtct
     3181 gtttttttat cagctgctag gatagaaagt gggtagttgg tgtcttgata agtgaaaaag
     3241 aaacgacgta aaaaggtttc aagtgagtct ttgtgattgg ctgtattttg taaatcaaag
     3301 ccacattttt ttagttcaga taagacattt tcttttggaa aattgatata actatattga
     3361 ttaaaacgca tagaacctcc atatagaatg acagttaagg ttattatatc aaaaaaaaag
     3421 cagaaaggga attgttaact tcaaaaggaa ataatccaat aaaaatgaat aaagtactaa
     3481 attcaatata gagaacagag taacaataag aataaataga tagggtataa aagttctagg
     3541 agatttatat tatatgcttt ctatttttat atacaatata gtataaatat aaaaatgatg
     3601 acaaaaatac aaatgaatag aaaataaatt agtaagctga tgaaattttt ctcaagagaa
     3661 gccatttata ggtgaaaatg gtataatata gtgagaagga tagaggagaa gtgtaaattg
     3721 atcgcacaac tagatacaaa aacagtctat agttttatgg aaagcgtcat ttcgatcgaa
     3781 aagtatgtga gagcagctaa agaatacggc tacactcatt tggctatgat ggatattgac
     3841 aatctttatg gcgctttcga ctttctagag attacaaaaa aatacggcat tcatcctttg
     3901 ctagggcttg aaatgacagt gtttgtagat gatcagggag tgaatttgcg ctttttagct
     3961 ctatctagtg tgggctatca gcagttgatg aagctttcga cagccaagat gcagggggag
     4021 aaaacttggt cagtcctgtc ccagtacctg gaggatatcg cggtcattgt gccttatttt
     4081 gatagagttg agtcgttaga actaggctgt gattactata taggggttta tccagaaaca
     4141 ctagcaagcg aatttcatca tcctatctta cctctttatc gggtcaacgc ttttgaaagc
     4201 agggatagag aagttcttca agttttaaca gcgattaaag aaaatctacc gctcagagaa
     4261 gttcccttgc gttcgagaca agatgtcttt atatcagcaa gttctttaga gaaactattc
     4321 caagagcgtt ttccgcaagc tttggacaat ttagaaaagc ttatttcagg catttcttac
     4381 gacttggata ctagtctgaa actgcctcgt tttaatccag ctagaccagc agtagaggag
     4441 ttgagagagc gtgctgaact ggggcttgtt cagaaggggt tgactagtaa agaatatcaa
     4501 gatagactag accaagaatt gtctgttatt catgatatgg gctttgatga ttatttcttg
     4561 gttgtttggg atttgttgcg ttttggacaa tcgaatggct attatatggg aatgggaagg
     4621 ggttctgcag taggcagttt ggtttcttat gccttagaca tcacggggat tgacccagta
     4681 gagaaaaatc tgatttttga acgctttctt aatcgtgaac gctataccat gcctgatatt
     4741 gatattgata tcccagatat ttatcgtcca gattttatca gatatgttgg taataaatat
     4801 ggtagtaaac atgcggcaca aatcgttact ttttcaacct ttggagccaa gcaagctctt
     4861 cgagatgtct tgaaacgctt tggtgtgcca gagtatgaat tatctgcaat tactaagaaa
     4921 atcagttttc gtgacaatct taagtcggcc tatgagggaa atctccagtt tcgtcagcaa
     4981 atcaatagta agttagaata ccaaaaagct tttgagattg cttgcaagat agagggctat
     5041 ccaaggcaaa cctctgtcca tgcggctggt gttgtaatta gtgaccaaga tttaaccaac
     5101 tacattcctc taaagtatgg tgatgaaatt ccactgactc agtatgatgc tcatggagtt
     5161 gaggctagcg gacttttgaa gatggacttt ctgggactac gaaatttgac ctttgtccag
     5221 aagatgcaag agttgcttgc tgaaacagaa ggtattcatc tgaaaattga agaaatcgat
     5281 ttagaagaca aagaaacgtt agctttattt gcctctggta atacaaaagg tatctttcaa
     5341 tttgagcaac caggtgccat tcgtctgctt aagcgtgtgc aaccagtctg ttttgaagat
     5401 gtcgtcgcga ctacttctct aaatcgaccg ggtgctagtg actatatcaa taattttgtg
     5461 gcaagaaagc atgggcagga agaagtgact gttctggatc cagtactgga ggatattttg
     5521 gctccaacct acggcataat gctctatcag gagcaggtta tgcaggttgc ccagcgactt
     5581 gccggattta gtcttgggaa agccgatatt ttgcgtcggg ctatggggaa aaaggatgcc
     5641 tctgccatgc atgagatgag ggcttccttt attcaaggtt cattagaagc tggtcatact
     5701 gtggaaaaag cagagcaggt ctttgatgtt atggagaagt ttgcaggtta tggttttaac
     5761 aggtcacacg cctatgccta ctcagccttg gccttccagt tggcttattt caaaacgcat
     5821 tatccagcca ttttttatca ggtcatgtta aattcttcca acagtgatta cttaatagat
     5881 gcacttgaag caggttttga agtagcctct ctatccatca acaccattcc ctatcacgat
     5941 aaaattgcca acaaggccat ctatctaggt ttgaaatcca ttaaaggagt cagtaatgat
     6001 ttagctctct ggattattga aaatagacct tattctaaca ttgaagattt tatagctaaa
     6061 ttacctgaga attatctgaa acttcctctg ctagaacctt tggtaaaagt tggtcttttc
     6121 gattcatttg aaaaaaatcg tcaaaaagta tttaataatt tagctaatct atttgaattt
     6181 gtgaaagagt tgggaagttt gtttggagat gctatttata gttggcagga atcggaagat
     6241 tggacggaac aagaaaaatt ttatatggaa caagagcttt tagggatagg tgtcagcaaa
     6301 catccactac aagctattgc aagtaaggct atttacccga ttaccccaat cggaaatttg
     6361 tcagaaaata gctatgctat tatcttggtt gaagttcaga aaataaaagt gattcgtacc
     6421 aaaaagggtg aaaatatggc cttcttacag gcagatgata gtaagaaaaa attggatgtc
     6481 actctctttt cagacttata tcgtcaggtt ggacaggaaa taaaagaggg agccttctac
     6541 tatgtaaaag gaaaaataca atcacgtgat ggccgtctgc aaatgattgc acaagaaata
     6601 aaagaagcag ttgctgaacg cttttggata caggtgaaaa atcatgaatc ggatcaagaa
     6661 atttcacgca ttttagaaca atttaaaggc ccaatcccag tcatcatccg gtatgaagag
     6721 gaacagaaaa ccatcgtttc tccccatcat tttgtagcta aatccaatga attagaggag
     6781 aaattgaatg aaatcgttat gaaaacgatt tatcgctaaa aatacggaaa atagaagaat
     6841 tttaaaatca aatgtgatat aatcaagaag aatgttaaaa gaaaaaggag cataaaccaa
     6901 tatgaaacgt attgctgttt tgactagtgg cggagacgca cctggtatga acgctgccat
     6961 ccgtgcagtt gttcgtcaag caatttcaga aggaatggaa gtgtttggta tctatgacgg
     7021 atatgctggt atggttgccg gtgaaattca tcccctagat gcagcttcag taggggacat
     7081 catttctcgt ggtggtactt ttcttcactc agctcgttac ccagagttcg ctcaacttga
     7141 agggcaactt aaagggattg agcaattgaa aaaacacgga attgaaggtg tagttgttat
     7201 cggtggtgac ggatcttacc acggcgctat gcgtttgact gaacatggct tcccagctat
     7261 tggtcttcca ggtacaatcg ataacgatat cgttggtact gactttacaa tcggttttga
     7321 cacagcggtt actactgcca tggacgctat cgataagatt cgtgatacat catcaagtca
     7381 ccgtcgtact tttgtaatcg aagttatggg acgtaacgct ggtgatatcg ctctttgggc
     7441 tggtattgca actggtgctg atgaaatcat catccctgaa gcaggcttca agatggaaga
     7501 tatcgtagca agcatcaaag ctggttatga atgtggtaaa aaacacaata tcatcgtctt
     7561 agctgaaggt gtgatgtcag cggctgaatt tggtcaaaaa cttaaagaag ctggagagac
     7621 aagcgacctt cgtgtaacag aacttggaca tattcaacgt ggtggttctc caactgcgcg
     7681 tgaccgtgtt ttggcgtcac gtatgggtgc acatgctgtt aaacttctta aagaaggtat
     7741 cggtggtgtt gcggttggta ttcgtaacga aaaaatggtt gaaaatccaa ttcttggtac
     7801 tgcagaagaa ggggcattgt ttagccttac tgcagaaggt aagattgtgg ttaacaaccc
     7861 acacaaagcc gatattgagc tatctagctt gaataagagc ttgtcataat ttacaattta
     7921 gttaataaga ccaaaaaggt catatatata aaggagtcac aaaaatcatg aacaaacgtg
     7981 taaaaatcgt tgcaactttg ggtcctgcgg tagaaatccg tggtggtaaa aaattcggtg
     8041 aggacggata ctggggtgaa aaacttgatg ttgaagcttc agctaaaaac attgctaaat
     8101 tgattgaagc tggtgctaac acattccgat tcaacttctc acacggcgac caccaagaac
     8161 aaggtgagcg tatggcaact gttaaacttg cggaaaaaat tgcaggtaaa aaagttggtt
     8221 tccttcttga tacaaaagga cctgaaatcc gtacagaatt gttcgaaggt gaagctaaag
     8281 aatattcata caaaactggt gaaaaaattc gtgttgcaac taaacaagga atcaaatcaa
     8341 ctcgtgaagt gattgcgttg aacgttgctg gtgctcttga tatctatgat gatgttgaag
     8401 ttggtcgtca agttttggtt gacgatggta aacttggtct tcgtgtggtt gctaaagatg
     8461 atgcaactcg tgaatttgaa gttgaagttg aaaacgatgg tatcatcgct aaacaaaaag
     8521 gtgtgaacat ccctaacact aaaattcctt tcccagctct tgctgaacgc gataacgacg
     8581 atatccgttt cggtcttgaa caaggtatca acttcatcgc aatttcattc gtacgtactg
     8641 caaaagatgt gaacgaagtt cgtgcaatct gtgaagaaac tggaaacgga catgttcaat
     8701 tgttcgctaa aatcgaaaac caacaaggta tcgataactt agacgaaatc atcgaagcag
     8761 ctgatggtat tatgattgct cgtggtgata tgggtatcga agtaccgttc gaaatggttc
     8821 cagtttatca aaaaatgatt atcaagaaag tcaatgctgc aggtaaagtt gttatcactg
     8881 caacaaacat gcttgaaaca atgactgaaa aaccacgtgc aactcgttca gaagtatcag
     8941 acgtattcaa cgctgttatc gacggaactg acgctacaat gctgtcaggc gagtctgcaa
     9001 acggtaaata cccactcgag tcagtaacta caatggctac aatcgacaag aacgctcaag
     9061 ctcttcttaa tgaatacgga cgtcttgatt cagattcatt tgagcgtaac tctaagacag
     9121 aagtaatggc ttctgctgtt aaagatgcta ctaactcaat ggatatcaaa ttggttgtaa
     9181 ctcttactaa gacaggtcat actgcacgtt tgatttctaa ataccgtcca aatgctgaca
     9241 tcttagcatt gacatttgac gaattgacag aacgtggctt gatgttgaac tggggtgtta
     9301 tcccaatgtt gacagatgct ccatcttcaa ctgacgatat gttcgaaatc gctgaacgta
     9361 aagcggtaga agcaggtctc gttgagtcag gcgatgatat cgttatcgtt gctggtgtgc
     9421 cagtaggaga agctgttcgc acaaacacaa tgcgtatccg cacagtacgt taagaaaaat
     9481 ataaaaacct atcatatcca gctttatagc ctgtatgatg ggctcttttt ttgtccaggg
     9541 ggcaaaaagg gggcaaaact ttcataatgg att
//
LOCUS       DAVDWT010000058         9402 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVDWT010000058 DAVDWT010000000
VERSION     DAVDWT010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.058"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(115..330)
                     /locus_tag="VUW50_001968"
     CDS             complement(115..330)
                     /locus_tag="VUW50_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983798.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNHEKFED"
     gene            complement(1214..2464)
                     /gene="ilvA"
                     /locus_tag="VUW50_001969"
     CDS             complement(1214..2464)
                     /gene="ilvA"
                     /locus_tag="VUW50_001969"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HET7983799.1"
                     /translation="MLSSKDIIKAHKILNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(2514..2870)
                     /locus_tag="VUW50_001970"
     CDS             complement(2514..2870)
                     /locus_tag="VUW50_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET7983800.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(3108..3344)
                     /locus_tag="VUW50_001971"
     CDS             complement(3108..3344)
                     /locus_tag="VUW50_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983801.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMNFEQFFKLAQI
                     SREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(3451..4473)
                     /gene="ilvC"
                     /locus_tag="VUW50_001972"
     CDS             complement(3451..4473)
                     /gene="ilvC"
                     /locus_tag="VUW50_001972"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET7983802.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(4539..5015)
                     /gene="ilvN"
                     /locus_tag="VUW50_001973"
     CDS             complement(4539..5015)
                     /gene="ilvN"
                     /locus_tag="VUW50_001973"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET7983803.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(5008..6708)
                     /locus_tag="VUW50_001974"
     CDS             complement(5008..6708)
                     /locus_tag="VUW50_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET7983804.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(6909..8576)
                     /locus_tag="VUW50_001975"
     CDS             complement(6909..8576)
                     /locus_tag="VUW50_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET7983805.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(8579..8944)
                     /locus_tag="VUW50_001976"
     CDS             complement(8579..8944)
                     /locus_tag="VUW50_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET7983806.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(9101..9289)
                     /locus_tag="VUW50_001977"
     CDS             complement(9101..9289)
                     /locus_tag="VUW50_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET7983807.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
BASE COUNT         2723 a         2112 c         1581 g         2986 t
ORIGIN      
        1 aaataaaaag aaaaggctgc atcaaccgtg cagtcttttt gctgtcctca aaatgaaaag
       61 gcagattctc tatctctact acttgaaggt caagaatccg ccgttatcca aagattaatc
      121 ttcaaatttt tcatgatttt tttcatagaa atcaattagg cctagggctg tttcaaatga
      181 aatatttttt acttttgcac gaccctgcgc cagagcaatg atagacattt cacgcgcatt
      241 cgtttcttta gagatacggt agcctgtgat tttcttatca cgaacccagc caacaactga
      301 ttctactttt tcaaagtttg acttagccat gtttttctcc tattttcttc tttataatat
      361 ttaattgtat acgtttttac tacttttgtc aataaaaaaa catgtattca acgaaaataa
      421 ttagttcaga attttagctt cagaattttc ttacttcctt tttgaatccg ataatatata
      481 ggttttacta gtctctacac cataaatatt tgctaataaa aatactgtat tattaccctc
      541 ttaaggtgcg aaattattca agttcttaaa aaatgcttct ctatttccgt aagatagctt
      601 atttcttcga tttaagaact gaagatgata gttctaacct ttagaaatcc aagccttcac
      661 tagctcaaaa actcctatct ttcttcctca ataaatcatc tgagagatag gtattcctta
      721 tatccttcaa attcatcact cctttggata ttgaggtgct agcctagaag cagacacccg
      781 attatctaaa ccatccttaa tagagatgac aagacagata agctcagtta attacttata
      841 tatcttttta atatggtata tttgtcaaca aaaagaaatc aaatcatacc ttatactcaa
      901 tgaaaatcaa agagcaaact aggaagctag ccgcaagttg ctcaaaacac tgttttgagg
      961 ttgcagatag agctaacgtg gtttgaagag attttcgaag agtattaaag acagtgtgac
     1021 tatgcttgtc aatagatagg aaatcaaaat gaaggatgga aatctgagcc aaacatcata
     1081 agtgctataa tgaacagaag taagaattgc acgataagtt tcctaaaacc atagaaatcg
     1141 tcccctttct tcatcttcat tatagcacat atacatcagt tgtacaaata atatgatatt
     1201 tttttattag tcctcagaca agcatattat aaagcgtttc attaccattt aagttaatat
     1261 aagctggatc aaaaccttcc attctacgaa tcaaacctgc ataatcatgc ttatctgcta
     1321 aagcgatccc aattaatact gggcctgttc ccttgctagc tcgtttgata tactcaaaac
     1381 gtgtgatatc atcatttggc cccaggatat catttacaaa ctcacgcaaa gctcctggac
     1441 gttgtgggaa attgaccaca aagtaatgtt tgataccatc ataaatcaag gcacgctctt
     1501 ccatttctgg catacggttg atatcattat ttcctccaga aatgatacaa caaatggttt
     1561 tccccttaat atattcagct aaaacctcta aagaggcgat actagccgct ccagcaggtt
     1621 ctgcgactat cccttgctta gagtaaaggt caatcaaggt ttcagaaatc aatccctcat
     1681 cgacacctac caaagtttta atatgttgac gagttgcttc ataggtcaac tgacctacct
     1741 tttgcacagc aatcccatcc gcaaatttat caatttcctt gagtttgact ggacctccag
     1801 cctcaaaggc agctttcatg gaacgcgctc cattcgcctc tactccgata acctcaatct
     1861 ctggacttgt ttccttgata taggtagaaa ccccggcaat gagaccgcca ccaccaacag
     1921 gaaccaagac agcatcaaaa tcaatcgatt cttttcgagc ttcttctaaa atctcataag
     1981 caactgttcc ttgacctgct tgaacatgag catcatcaaa aggatcaata aaggtacgat
     2041 tttcagagac tgtaaattct tgagctgctt tggctgaggc atcaaaggta tctccaacta
     2101 gtttaatagt tacaaaatcc ccaccaaaaa agcgaacctg accaatcttt tgttgtggcg
     2161 tagtaatagg cataaagata gtagcaggaa ttttcatttc attacaagta taggctactc
     2221 cctgcgcatg atttcccgca gaagcgcaga ctaccccacg ttcacgttct tccttgctga
     2281 gctgggaaat ggcataatag gcaccacgaa ttttaaagga gcgaacacgc tgggcatttt
     2341 cttttttcaa ataaatctta gcaccatact tctccgataa ataatgatcg taatccagtg
     2401 gagtattcac aaccacaccg ttcaagatct tgtgagcctt gattatatct tttgaactta
     2461 acatataaaa cctctttttc tataaaaaac agtcactaat gactgttttt tagttattca
     2521 tctccgaaaa cttcagtaac actttcatgg tatttatgag ccatacgaat cgaaaactct
     2581 ttaggaagct cataacgatt tgtggaaatc caatcatata accattctac caaactttct
     2641 tcactatcat tacgatggaa gataaaatag tttaaaattt ctttatattc tacatcattc
     2701 aaatccaacg tgtctacaaa ttgactacga ggaacaaaat tagaatctaa ttttcttagt
     2761 tgatctgtca attctcccaa ttgtaaatcc aactgaaaaa atagtttttg cagttgccta
     2821 tcagtttcat cacatcctgt cttgatagaa aacaaattgg taatattcat atgatttcaa
     2881 cccctaaata agtacatcta atatcccacg aatagctgaa gctaaagttt tcgcagtcca
     2941 gtcatttaaa cccgattctt tcaatccaga tgcaataatt tgctctattt gatataaacc
     3001 gccatctatc gtctcattta ttctgcgaag aggaccttca atataggata aagcttctcc
     3061 gaatctaaca gctgatttag gtcctaatga tttaccaact atatttgtta atgtttttta
     3121 tttctcaaaa tccatttcgc tatataacga gcagatttag tcgcaccagc tctctcaaac
     3181 ggagaatgaa tttcaagacc ttcttctcta gatatttgtg ccaatttgaa gaattgttca
     3241 aagttcatat gcaacctacc atcttcaaaa ttagcatcat cataaatgtt aattacagca
     3301 ttttcatcat acgaccaatc tgctttattt tggtctgcta gcactattac cgacggtgta
     3361 attacattat acaactagaa ccaataataa cgatgcaatt tttctcataa tctttttcat
     3421 aaaatcatct ccagcgctat atatttctaa ttagttatag attttgaatg catcatcgtc
     3481 gtttttacca acgaatggca ttgctttacg caattctgca ccaacttttt caatttcaag
     3541 gttagctgct tgttcacggt aagcagtcaa ttttggacgt ccagctttat agtcatttac
     3601 aaagtcattt gcaaatttac cattttggat gtctgccaag acagccttca tattttcttt
     3661 aacttgttca gtgattacac gtggacctga tacatagtca ccgtattcag cagtgtttga
     3721 aatagattga cgcattttct tgaatccacc ttcgtagatc aagtcaacga tcaatttcat
     3781 ttcgtgaaga acttcaaagt aagccaattc tggagcgtaa cctgcttctg tcaagacttc
     3841 gaaacctgct tcgataaggg cagtcaaacc accacaaagt acagcttgtt caccaaacaa
     3901 atcttcttca gtttcttctt tgtaagttgt ttcaagaaga cctacacgag ccgctccaac
     3961 acctttacac cagtccatag caatgttttt agcatttcct gttgcatctt ggtatactgc
     4021 ataaagagct ggaacaccaa atccttcttc gtaagtacga cgtaccaagt gtcctggtcc
     4081 tttaggagca cacatgaaga catctacatc cgcaggaact ttgataaatt caaagtggat
     4141 gttgaaacca tgggcaaatc caactgcgtt tccagcttcc aagtttggag cgatttctgc
     4201 ttcgtacaat tcttgttgaa tttcgtctgg cgccaagatc atgataacat cagccaactt
     4261 agtagcttct gctactgtgt aagtatcaaa tccatcttct tttgctttat caaaagattt
     4321 acctggacgt acacctataa taacgtcacg acctgaatca cgcaagtttt gagcatgcgc
     4381 atgcccttgt gaaccataac cgataacggc gattttttta ccgtcaagtg ctgctacttt
     4441 aacatctttt tcatattcca tttgaactgt catagctttt ctctcttttc tattatttat
     4501 tgccttttag gctggtttaa taaatttaag ttggattttt aatcgcgggt aaatccagtt
     4561 gcacccgttc gagcaatatt gcgaataccg tatgggcgaa tgactctcaa tagggcttcg
     4621 ctcttttctg catttcccgt catctgaatg gtaatcgagc ttggcgctac gtctaccact
     4681 gttgcacgga aaggttgaat aatcgctaaa atctcagctc tcttctcagc tggcgctgac
     4741 atcttaacca aaatcacctc gcgctccaaa tgaggcttgt ctgtaatatc tcgaatgcga
     4801 atcacatcaa tctgacgatt gagctgtttg atgatttgct ccacttcatc atgagaagca
     4861 acatcaataa taatagtgat acgcgataca ttcggatctt ctgttgctcc aacagagatg
     4921 ctttcaatat taacctgacg acgagatagg acacctgtaa agcgattgag gactcctgaa
     4981 cgattttgta gttttgctgt taacattcta cgcatggaac tgcaccccca acatctcatg
     5041 attactctta ccagccggta ccattggtaa cacctgttcc ttacgagaaa tatctacctc
     5101 aattagcata ggaacatcct cagtgatgac ttcaaggtct tgagccaagg tctcaggatt
     5161 atcaaactta tagtttttaa taccataagc ctgcgccatc aattggaaat caggaagggt
     5221 atcaaagacc gactctgatg ttctgccttc atagaaggat tcctgccact ggcgaaccat
     5281 tccaagtgaa tgattgttca gcataaccac cttgattggc accttgtaaa tattcaaaat
     5341 agccaactcc tggttggtca tttggaaacc accatcccca acaaacaaga ctacttcctt
     5401 atctgggtta gcaattttag caccgattgc tgctggaatt ccaaagccca ttgttcccaa
     5461 accacctgaa gtcactaact gacgttcatt ttggtaggga taatactgag ctgtccacat
     5521 ttggtgttga ccaacgtctg ttaccacaat ggcatctcca ttcgtcaatt caccaattcg
     5581 ttcaataact gcttgcggtt gaaccacacg ctctttcttg tcataagaac gaacacgatt
     5641 cttgtcttta gtgactttct caatccactt ttcagtgttg ttgtgaactg ttggttctgc
     5701 tagcaacatt tgcaaggcct tcttagcatc tccaactaca ggaatgtctg cactgataat
     5761 cttgccaatc tcagctgggt caatatcaat gtgggcaacc ttagcattct tagcgaaagt
     5821 cttaggattc cccgtcaaac ggtcatcgaa acgagaacca atactaatca taaagtccgc
     5881 ttccgtcatg gcaatatttg ctgcgaatga cccgtgcatg cctcccattc caagaaagag
     5941 tgggtgactc gttgcaatcg ttccttgtcc caaaagactg gttaccactg gaatttgata
     6001 gcgttctgca aattcattta gttccgtagc agcctcagca taactaattc caccaccagc
     6061 taacaagact ggctttttag ccttggacaa ttgcttcaag attttcttga tttgcatatc
     6121 attcggctca agagtcggct gataacttgg taaattcact tctggtgaat aaatgaagtc
     6181 tgtttctaaa gcagatatat cttttggtag gtcaattaca actggccctg gacggcctgt
     6241 agttgcgata tggacagctt ccgtaatgat acgcggaata tcagctgtct cacgaacttg
     6301 gtaattgtac ttagtgattg gcatggtaat tcccacgatg tctgcctcct gaaaggcatc
     6361 cttcccaatc cctgctcgcg ccacctgacc tgtaaagacc aaaaggggaa cgctatcgct
     6421 catggcatcc gcaatccctg taatggcatt tgttgctcct ggtccactag tgacgacggc
     6481 aacacccaac tttccagttg atttggcata accttcagct tcatgcaaac aaccttgctc
     6541 atggcgccct agaatgtggc gaatgccttt aaaattatat atcgcatcat aaaaaggcaa
     6601 aaccgcacca ccaggataac caaagatggt atcaactcct aaatcacgaa gtgtttccaa
     6661 aactaggtcc gaccccgtct taggagattc taaactgatt ttctccattg ttcccctttc
     6721 tttcctctta aaaataactt gttactatca taccattttt tcaaaatttt tcaagataaa
     6781 agaagaaatt ttctgaattt tctattttaa cgtttattta tgaatgttat ttttgttttt
     6841 attaaaaaga taccgatttc atttactaaa aaagaaaaaa gaactgattt ctcagtcctt
     6901 cattaatctt attccacact aaataggtat gggtaaacag gttgttgacc ttggtgaatc
     6961 tcgacttcaa cgtcttcgaa ttcttctacg atttcttgag cgatttcatt ggcaagttct
     7021 tcgcttccgt cttcaccgac atagaaggtt acgatttcac tgtcttcatc caacatatgt
     7081 ttcaaggttt cagtcaatgt ttggtgcata tcagggtttg acacaagaat ttttccatcc
     7141 accataccta gattatcgtt ttcatggatt tctaagccat cgatcgttgt atcacgcacg
     7201 gctgttgtga cgcttccgct aacgacatcg ctaagagcag ctgtcatacg ctcttggttt
     7261 tcttcaatgg acttgcttgg atcaaaggca agaagacttg tcataccttg aggaagagtg
     7321 cgagcctcta ccactactgc tggttgctcc aaaacttctg ccgcagattg agctgccatg
     7381 aagatgttct tgttgtttgg caagaagatg atgttacggg cattgacctg ttcaacagcc
     7441 ttgataaagt cttctgttga agggttcatg gtttgaccgc cttcgataac ataatccacg
     7501 ccttgagaac agaagatatc tgctagacct ttaccagcca ccacagcaat caaagcatac
     7561 tctttttctt cagccgactt gataacttga gtagcttctt tctcaacctg tgcttcgtgt
     7621 tggttacgca tattgtcaac ttttaccttg accaagctac catatttgag accttcttgc
     7681 ataacaagtc ctggatcttc tgtatgaaca tggactttga caatttcatc atcgttaaca
     7741 acgaggagag aatccccaag ctcatccaag tagttacgga attcatcgta gtcaaaatct
     7801 ttagcatagg ttggaccttg cttaagagct accatgattt cagtacagta accaaacgtg
     7861 atgtcctcag tcgctacgtg accagctaca gacttatgat gctctacatt gatcatctca
     7921 ctcatgttgg caggagtcgc tacaaagtcc tcagatgcaa tatattcgcc agtaagggct
     7981 gaaaggaaac cttcgtagat gaagaccaat ccttgaccac ctgagtccac aacgccaact
     8041 tctttcaata ctggaagcat gtctggtgtt ttagctagag ctgttttagc accttccaag
     8101 gctgcgcgca tgacttcaac agtatcatct gtttgctcag cttttttctt agcaccgata
     8161 gcagctccac gagaaactgt caaaatcgtt ccttcaacag gtttcatcac tgccttatag
     8221 gcaacttcca cacctgattg gaaggccaga gccaagtctt gacctgttaa ctcgtcttta
     8281 tccttgatag cttgggaaaa tccacggaaa agctgagatg taatcactcc tgagttccca
     8341 cgcgcaccca tcaaaagccc tttggcaaga atgctcgcta cttctccaac tgtagaagct
     8401 ggcttgtctg caacttcttt agcaccattt tcaatggtca ttcccatatt tgtcccagta
     8461 tctccatctg gaactggaaa gacgtttaat gaattgacat attcagcttg cttattcaag
     8521 cgagttgatg cagcctgcac catttcttga aataagctag tagtaatctt tgacacggtt
     8581 attctcctac aactttgata ttttgaatgt agacatttac agtctgagca gtaattccaa
     8641 gctggttttc caagctaaag gcaacacgct cttgaatgtt ttttgacact tcactaatct
     8701 ttgttccgta gcttaacacg gtatatacat caactgcaat actgccatct tcggctgcct
     8761 ttacgacgac acctttagaa taattttcct tacctagcag ggcttggaaa ttatctttga
     8821 gggcattttt actagccata ccgaccacac caaaaatctc agttgctgct ccacctacga
     8881 cggttgcaat cacttcatct gttagttcga tttgaccatc ttttgtatta atttttacag
     8941 tcatcctttt tacctcaact agttgatact ctattttatc atatttcagc ccaagtgtaa
     9001 aagcagaatg atgtatcagc ggatatttac tctatttttc aaacgatgtt atacccacaa
     9061 taaaagaaaa aagaccctaa ggtctccttt gcttttatta ttaaacgcgt tcaactttac
     9121 ctgatttcaa agcacgagct gaagcccaaa cttttttagg tttaccatcg ataagaacag
     9181 taactttttg aaggtttggt tttacggcac gttttgtttg gttcatcgcg tgtgaacggt
     9241 tgtttcctga tacagtctta cgacctgtaa agtaacatac tttagccatt gtgttttcct
     9301 cctattagat ctaatatagc ggatgtgcta gcaccacata ccgtactatg ttatcacact
     9361 ttcttgtttt ttgcaaggga attggaagat tttttatttg tg
//
LOCUS       DAVDWT010000059         9239 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVDWT010000059 DAVDWT010000000
VERSION     DAVDWT010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9239
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.059"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(411..1607)
                     /gene="tuf"
                     /locus_tag="VUW50_001978"
     CDS             complement(411..1607)
                     /gene="tuf"
                     /locus_tag="VUW50_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET7983808.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2528..3397)
                     /locus_tag="VUW50_001979"
     CDS             complement(2528..3397)
                     /locus_tag="VUW50_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET7983809.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYTATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3620..4141)
                     /locus_tag="VUW50_001980"
     CDS             complement(3620..4141)
                     /locus_tag="VUW50_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET7983810.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEPGTPKTETPVNPDPEVPTYATGKREELPNTGTEANATLASAGIMTLLAG
                     LGLGFFKKKEDEK"
     gene            complement(4099..5205)
                     /locus_tag="VUW50_001981"
     CDS             complement(4099..5205)
                     /locus_tag="VUW50_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET7983811.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEVDTNSASLTGNIVSEGRTVTLVYRESEAPTTVTVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKENRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5316..5570)
                     /locus_tag="VUW50_001982"
     CDS             complement(5316..5570)
                     /locus_tag="VUW50_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983812.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(5910..6356)
                     /locus_tag="VUW50_001983"
     CDS             complement(5910..6356)
                     /locus_tag="VUW50_001983"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7983813.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERISIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDLLREVPTIFECLECFFKTR"
     gene            complement(6429..6754)
                     /locus_tag="VUW50_001984"
                     /pseudo
     CDS             complement(6429..6754)
                     /locus_tag="VUW50_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6988..8706
                     /locus_tag="VUW50_001985"
     CDS             6988..8706
                     /locus_tag="VUW50_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET7983814.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            complement(8756..>9239)
                     /locus_tag="VUW50_001986"
     CDS             complement(8756..>9239)
                     /locus_tag="VUW50_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HET7983815.1"
                     /translation="SHEYKAIKRYWKLIQQDSRKLSDKHFYRPTFRMHLTNKEILDKL
                     LSYSQDLKHHYQLYQLLLFHFQNKEPEKFFGLIEDNLKQVHPLFQTVFKTFLKDKEKI
                     VNALQLPYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSQA
                     "
BASE COUNT         2647 a         1865 c         1743 g         2984 t
ORIGIN      
        1 ttggatttct tctttagttg atttcatatt actattgtat aatgcttttt gatttgagtc
       61 tagtattata ctcttcgaaa atctcttcaa accgcgtcag cttcgccttg tcgtatagat
      121 gttactgact tcgtcagttc tatctacaac ctaaaaacag tgttttgagc agtctgcagc
      181 taactttcta taacaaaatg aaacaagaac aagacaaatc aatcaagaca atcaaatcga
      241 tttctaacaa tgttttagaa gtagaggtgt actattctag tttcaatata ttatagtttg
      301 ctctttgatt ttcatttagt attagttcaa tatctaaaaa aagaaccttg ccaagcaaga
      361 ttcttttagt gtctgactta aataattgtt cttctgggaa ctaaatcgaa ttaagcttcg
      421 atttctgtaa ccatacctga accaacagta cgtccaccct cacggataga gaatgtagta
      481 ccttgttcta cggcgattgg gtgaatcaac tcaacatcga ttgtcacgtt atcaccaggc
      541 attaccattt cagtacctgc tggaagttcg attgaacctg taacgtcagt agtacggaag
      601 tagaattgtg gacggtagtt gttgaagaat ggagtgtgac gtccaccttc ttctttagta
      661 aggatgtaga cttcaccttt gaatttagtg tgtgggttga ttgaacctgg tttagcgata
      721 acttgtccac gttcgatttc atcacgttga acaccacgaa gaaggacacc tacgttatct
      781 ccagcaagac cttcgtcaag ttgtttacgg aacatttcaa caccagtaac aactgctttt
      841 tgagtttctt ctttgatacc aacgatttcg atttcgtcgt tgactttaac gataccacgg
      901 tcgatacgtc ctgaagcaac tgtaccacgt ccagtgattg agaatacgtc ctcgactgga
      961 agaagcaatg gtttgtcagt gtcacgttct ggttctggga tatactcatc aactgtgttc
     1021 atcaattcca taacgatgtc ttcgtattta gagtcacctt caagagcttt aagtgctgaa
     1081 ccttggataa ctggaagatc gtcacctggg aagtcgtatt ctgacaatag gtcacggatt
     1141 tccatttcaa ccaattcaag caattcttcg tcgtcaacca agtcaacttt gttcatgaag
     1201 acgataaggt gtttaacacc aacctgacgt gaaagaagga tgtgctcacg agtttgtggc
     1261 attggtccgt cagttgaagc tactacaagg atagctccgt ccatttgagc agcaccagtg
     1321 atcatgtttt taacgtagtc cgcgtgtcct ggagcgtcga tgtgagcgta gtgacgtttt
     1381 tcagtttcgt actcaacgtg cgcagtgttg atagtgatac cgcgttcgcg ttcttctgga
     1441 gcagcatcga tagacgcata gtctttaggt tggttaactg atgaaggcaa gcgacgtgcc
     1501 aaaacagttg tgatagctgc agttagggta gttttaccgt ggtcaacgtg tccgatagta
     1561 ccaatgttaa cgtgtggttt actacgatcg tatttttctt ttgccatttg agtaaaagcc
     1621 tccaataaaa tatattttat agatagacag taggcaatac agtctaactt tccttactat
     1681 tttatcaaat ttcagctaaa ttgcaagtgt tttacaacgt ttttgtgaat tattcttggt
     1741 aaaaatttaa ctaactaata gaaagtacta aatagactta tccttcaagg agttttcagc
     1801 attatccatc cactcacttg ctgaaaatgt aaacagtctc tggtttatat agtaaaatga
     1861 aacaagaata gggcaaatcg atcaggacaa tcaaatcgat ttctaacaat gttttagaag
     1921 ttgaggtata ctattctagt ttcaatctac tatacaacct caaaactgtg ttttgaacaa
     1981 cctgcggcta gcttcttagt ttgccctttg attttcattg agtataaaat tgcagataaa
     2041 acgataacaa tcataggact cagcagagtc aatgtaggtc gaagaatagc cccctaatcg
     2101 cttattttat agcaaaatga aatagaaact gaacattttg ttgaggaagg taaatcaatt
     2161 cctcacaatg tttgagaaag aagggaggac tattccaact tcaacccact ataaagcaga
     2221 cttacaagat agggaaccca tctccgataa tattccgata atatatagta gaatgaaaac
     2281 tgcacaattt gattggagaa ttcaaaccaa tttctaacaa tgttttagaa acaagggtct
     2341 attatttcaa ctgcaaccta ctatagccca ttgaagctgg aatagcccac tgagacttct
     2401 aaaccactgc tggagaggag tttgtttaga gtttctttca tttccctaaa aaatcaaaaa
     2461 gagagaacca gtctgatcct ctcttactta tagatggtgc tcactcaaat gttaaaggag
     2521 ctatttctta gagataaagg aatttgaata cagctactgc cgcaattgct gctgcgatag
     2581 gtgctactac tggtacccaa gaataccacc attttgaatc gcctttatgc tcaccaagaa
     2641 ctgattttgg aaggaaagca tgaaggagac gtggtcccaa gtcacgggct gggttcaagg
     2701 caggtcctgt aggtcctcca agtgatgtta ccaaagccat aacgaggaat ccaagtgcca
     2761 agtgagccac agaaagtcct gaagcagtgt gtggagccac ctgtgcttta atagccaagt
     2821 cagaaaaatc aactgtttgt cctgcttcta ttgccttttg tttcatgaat tgaagcactt
     2881 cagcaccaaa gaagttttta gtcaaaccaa gagctgcaaa gaacaaaaca aatgaaccaa
     2941 caaactcatt aatcaaacca ttgacagttg ctgtatagcg actttctttt gtaccatggt
     3001 caatacttga aatagttgag aaagttccca agatgttatt tggtttttca gttttcaagt
     3061 agaatggacg gtatgttgcc acaactaagg cttggccaaa gatagccccc aagacttgcg
     3121 cgataatgta aggtaccact tgtgcccaag ggaaaagacc gctaactgca agccctagag
     3181 tgaaagcagg gttgatgtga ttcccagata cgttaccaaa catcaaggct gggatcataa
     3241 cccccatacc ataaccaaca gcgatgacga tccagccact ttggtgacct ttcgtacctt
     3301 taagttcaac gttggcaact gcaccattcc caagaatgat caaaatggca gttcccaaaa
     3361 attcagtggc atacttcagt gcccatgtga aatccatttg ctagattctc cttaaaattt
     3421 tttagatatt ccttatttta tcaattttta ccctatttag caactgattt ctggaaatat
     3481 ttttaaaaaa ataggattca tagtttccca gttcaacgta cttgtaagcg tttttattct
     3541 tcacgaaaga cctcattctc aaaaccagga aaaagcccac atctatgagc ttttcctggt
     3601 tcctagattc taaaatctat tatttttcat cttctttttt cttgaagaat cctaatccta
     3661 gaccagctaa caaggtcatg ataccagcac tagccaaggt agcattagct tctgtacctg
     3721 tgtttggcaa ttcctctctc ttacctgtcg cataagtcgg aacttctggg tctggattca
     3781 ctggagtttc agtttttgga gtacctggtt ctggagttgg tttatctggt gttgataaac
     3841 ggtcatacct taccgttatt tctttatcac tagagtctga cgtaacttct tgtgattcaa
     3901 ctgttggaat atctggatct ttgtacttgt caatcttacc agatataacc tcgtcccagt
     3961 ttcctgttgt ccattcacca taggttacaa ctcccgtgac cttgttctca gtttttgtac
     4021 ggcttaaggt tacaggttga acaacatctt cttttacatt ttggttcgta actttatcaa
     4081 cgtaatgaat gatacgcgtt atagtcttcg tctcagtaga ggttgctgtt ttgggaacca
     4141 ctgtttcctc aacattctaa cggtagtaat agtcaactgt tgcaccgtct tctggtacgc
     4201 atttgcagga gttgctacca aggtgtatgt tgttttcctt gtgataactc ggttttcttt
     4261 gtcctcagtt gttgttttcc cttcaatagt ttttgattct gtggtatact cagaacctat
     4321 cgctaaatca gcttttataa cagactctgc caacttctct tggctacctt ctttatagta
     4381 attggctgtt actgtaacag tggttggcgc ttcgctttct ctataaacta aggtcactgt
     4441 tctaccttcg cttacaatat tcccagttaa acttgcagaa tttgtatcta cttctttaaa
     4501 agtataatat tttccgtcag tagtagtcat gctactgagt tttttatctg tgacattgta
     4561 gctggtacca atcagttgtt ttttattggt aatgtaggtt ccgtcacttt ctttttctcc
     4621 aattccagta tcatttttca atgatagcaa acgcccttgt tcatcaacat agcgaacttt
     4681 cacattttct gagattagtt ctgccaattc tgaggttttt ttctttttct tgatttcttc
     4741 ggttattttc cctttctctt cttcgggaat atttagtttt ggaatgattt tttcaacaac
     4801 ggttcgtgat ggttccacag tatcttggat gactgaaaag tcagctagaa ttgggagatt
     4861 atactgaaca cggtgacttt gagtgtttac tcctactctt tcattattct ctgaaaatac
     4921 ttgtacggta taagaaacaa catcttttcc taatagaaca tccccagtag agaaatagcc
     4981 gccttttcct agtttgctat ctccagagcc cacttctttc ctaatcttat cagatagttt
     5041 tttaccagtc agtacattcg ttcgcacaat ccctttgtct acccctacaa agtgggagaa
     5101 ctttttgaac tcttcagaac cagatctagc ccaaccatta ttaagggcat ttgcttttgt
     5161 atttgtattc tctctcaaaa gtttggcgat tagaattata ttcatcggca cttagagttg
     5221 ctgctatatc tgactcttga ataccaactt ccttactacc atttctagcg gcagtatatg
     5281 tgtatttatc tgtttctact tctagctgat tatccttaac atgataggta attttctcct
     5341 ggtataagag tcttcagtag tttatatgct ctgtcatcat ctttagcttt agaagcagct
     5401 gagtttagcc aagttggggt attaggagta tctttatcag cctgtttcaa gacattattc
     5461 aagtcatttg ctagcctatt ggttgtcctc actgtgaaag ttttcttcca gtctgaacca
     5521 cttttattag acttagatat gctcatgaaa tcctggtcaa cattcaacac aatatcgcct
     5581 tgttttttag catcttccgt tgcttctgct tcaaattgat catggagttt ttgttgctct
     5641 ggtgtatagt ttttttattt acacgattta atgtactatt ttttatttta gtattaagaa
     5701 ctccattaat acgattttta aactctgctt caaaaaatcc cttaatttgt tggtgcgcaa
     5761 gttcaacatt caaacctttt ttatacaagt cttcattctg aacgatttcg gctacacggt
     5821 gaatggtttg attgtatacc gtcttctctt aaccagcagt atccttagtt ttaatgacta
     5881 ctcgataggt tcctttagaa ccgttatagt catctagttt taaaaaagca ttccaaacat
     5941 tcaaaaatag ttggaacttc ccttaataaa tccgtcactt tctttttcaa gatagcccaa
     6001 gcttgctcaa taggattcaa atccggtgaa taaggtggta gaggtaagaa aaagtgttta
     6061 tcttggatgc agagttcatc caaaatgttc ttgggatgaa aaccagcatt gcccatgaca
     6121 ataacatgag gtgtcttcaa agcaggtagg agttgcgttt tgaaccactc cacaaagaaa
     6181 tcgcttgtca tgcttttctt gtaaatcatg ggagctataa actctccgtc tacttgtcct
     6241 gcaacaattg aaattcgctc aaaacgacgt ccgctaatct tttcatagac tttctcccct
     6301 ctaggagccc ctgcataagg acgatagaga tagcggtcga ttcctgtttc atcaatataa
     6361 acaggtgcta ggtgctttaa actattaaaa ttcttaagaa ataaggctac ttttctgggt
     6421 cttgttcctt aaagctcgtc atcttttttt aaagtgacct taatctgctt taaagctgcc
     6481 catactgaag gaacagcaca atcaaaatgt gccgcaattt cccgtaaaaa agcatctgga
     6541 tgagcctcta caaaggcttt caattcctct aaaggaatct ttcggttttt gacgactcgc
     6601 tttttcctct ctatgtgttc ttgttcacgt ctttcttttc ccacgtgaag agagttctga
     6661 cgccaacacc aaaaaacttg gctgcctcga catggctgtg cccctctttg atgtaatcta
     6721 atgctcgctg tttaaaatca gtactatatg ccatagcttt attataatat gcttgtttaa
     6781 aactaagtaa ctatatcaat gttttgttat gagaagactt acttttttta ctgaaattga
     6841 atttttccta gcctaaacat catataaccc caaaattcaa tctgacctgg tttgaagaga
     6901 ttttcgaagc agataaggtt ttactaaaac taaaaaaaat gatataatca aactgattaa
     6961 atgttttaaa aataaaggag aatttgaatg tcttaccaag aaaattacca gaaatgggtt
     7021 gattttgtgg agcttcctga ctaccttcgt caagatttgg aaaatatgga cgaaaaaact
     7081 aaggaagatg ccttctatac aaatcttgaa tttggtactg caggtatgcg tggcttggtt
     7141 ggtgctggta caaaccgtat caacatctac gttgttcgcc aagctactga aggattggct
     7201 cgtttgattg agtcaaaagg tggaaacgag aaagaacgcg gtgtagcaat tgcctacgat
     7261 agccgtcact tctcacctga gtttgccttt gaatctgcgg cagttcttgc taaacacggc
     7321 atcaaatctt acgtatttga aagccttcgt ccaactccag aactatcatt tgcagttcgt
     7381 catctcaact gtttcgcagg tatcatggtc acagccagcc acaaccctgc tccatttaac
     7441 ggttacaagg tttacggtga agacggtgga caaatgcctc cacacgatgc ggacgctttg
     7501 actacttata tccgtgcaat cgaaaaccca tttgcagttg aagttgctga tgtggaaact
     7561 gaaaaagctt ctggcttgat tgaagttatc ggcgaagctg ttgacataga ataccttaaa
     7621 gaggttaagg acataaacat caacccagcc ttgattgaag aatttggtaa agacatgaag
     7681 attgtctaca caccacttca tggtactggt gaaatgttgg ctcgtcgtgc tcttgcccaa
     7741 gcaggatttg actctgttca agttgttgaa gcgcaagcaa ctgctgaccc agacttctca
     7801 actgtaacat ctccaaaccc agaaagccaa gcagcctttg cccttgctga agaacttggt
     7861 cgtcaagttg gtgcagatgt tcttgtcgca actgacccag acgctgaccg tgttggtgtt
     7921 gaagttcttc aaaaagatgg tagctacctc aacctttcag gtaaccaaat cggtgctatc
     7981 atggctaaat acatcttgga agctcacaaa aacgctggaa ctcttcctga aaatgccgct
     8041 ctctgcaaat ctatcgtttc aactgacttg gtaacgaaga ttgctgaaag ctacggcgca
     8101 actatgttca acgtcttgac aggtttcaaa tttatcgctg aaaaaatcca agaattcgaa
     8161 gaaaaacaca accacactta catgatggga tttgaagaaa gcttcggtta cttgattaaa
     8221 ccatttgtac gtgataaaga tgccatccaa gccgttcttg tcgttgctga acttgctgcc
     8281 tactaccgtt ctcgtggttt gacacttgct gacggtatcg aagaaatcta taaagaatat
     8341 ggctactacg cagaaaaaac aatctctgtt actctttcag gtgtcgatgg tgctgaacaa
     8401 atcaaagcga ttatggctaa attccgcaac aatgctccaa cagaatggaa cgcaacagct
     8461 atcactgtcg tagaagactt caaggcacaa actgctactg ttgctgacgg tactgttaca
     8521 aacttgacaa ctcctccaag tgatgtgttg aaatacacac ttgctgacgg ttcatggatt
     8581 gccgttcgcc cttcaggtac agaaccaaaa atcaagttct acattgcagt tgtaggggaa
     8641 accaacgaag aatcacaagc taagattgct aacatcgaag cagaaatcaa tgcatttgta
     8701 aaataaagac ctataaaaga tggctttttg tcaactgtag tgggttgaaa aaaagctaag
     8761 cttgagaaag gacaaatttc gtcctttctt ttttgatatt cagggcgata aaaatccgtt
     8821 ttttgaagtt ttcaaagttt cgaaaaccaa aggcattgcg cttgataagt ttgatgagat
     8881 tattggtcgc ttccaatttg gcgtttgaat agggtagttg aagggcgttg acgattttct
     8941 ctttgtcctt tagaaaggtt ttaaagacag tctgaaaaag aggatgaacc tgcttaagat
     9001 tgtcctcaat aagtccgaaa aatttctctg gttccttatt ctgaaagtga aacagcaaga
     9061 gttgatagag ctgatagtga tgtttcaagt cttgtgaata gctcaaaagc ttgtctaaaa
     9121 tctctttatt ggttaaatgc atacgaaaag tagggcgata aaaatgttta tcgctgagtt
     9181 tacgactatc ctgttgtatg agcttccagt agcgcttgat agccttgtat tcatgagac
//
LOCUS       DAVDWT010000060         8930 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVDWT010000060 DAVDWT010000000
VERSION     DAVDWT010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8930
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.060"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(383..532)
                     /locus_tag="VUW50_001987"
     CDS             complement(383..532)
                     /locus_tag="VUW50_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983816.1"
                     /translation="MTNYDRAKRFYSRATYVLSDFLHGRIDEQEFRKQWQRLADKAVN
                     ETKKN"
     gene            complement(519..665)
                     /locus_tag="VUW50_001988"
     CDS             complement(519..665)
                     /locus_tag="VUW50_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983817.1"
                     /translation="MEQYTKRASALIITFNRGVISQDEFIEEFTKLREKVVKAVSEAK
                     NDKL"
     gene            complement(669..1097)
                     /locus_tag="VUW50_001989"
     CDS             complement(669..1097)
                     /locus_tag="VUW50_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983818.1"
                     /translation="MGKKHQVVKFKDIAEKLPELEGKRLEEITKVLGYRNLESCRTNL
                     CKLKQNNRLDFTLEKGVYSKFALLDGTVKEELEDKELSERGRYLKSVDRYKAMLNAFS
                     IAFDSTVKAETRQKAEHDGLKALDRIPDKHYALLYDMMEG"
     gene            complement(1203..1463)
                     /locus_tag="VUW50_001990"
     CDS             complement(1203..1463)
                     /locus_tag="VUW50_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983819.1"
                     /translation="MENEFKTVTNAKGVEIPKYFKDFKKLVEMDRQLAEYLCMNYELL
                     DSEDLGAFLETVEQGFSWILDLIESKDLLYKPKSGSNHAKRK"
     gene            complement(1475..2065)
                     /locus_tag="VUW50_001991"
     CDS             complement(1475..2065)
                     /locus_tag="VUW50_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HET7983820.1"
                     /translation="MVDGKKEPYTLSSIVAEYAEIKHRHLKILLNKHREDFESFGKVQ
                     FKISPSESGQNVRDYILNEQQATLLITYLRNTEPVKEFKKDLVKAFFEMRDELSKRYL
                     QRELEKPKRKTLTEAIKSWEKAPQHAYSTLTNLLLKGATGKNKAQLMQERESENGIDS
                     LTSAELTNYQRLEDMAIAMINLNMRYSEIKELIFKV"
     gene            complement(2103..2249)
                     /locus_tag="VUW50_001992"
     CDS             complement(2103..2249)
                     /locus_tag="VUW50_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983821.1"
                     /translation="MEDKIIELADYFISENTTYREAKIACEKLFLQVSHEIELRALES
                     ETKV"
     gene            complement(2655..2840)
                     /locus_tag="VUW50_001993"
     CDS             complement(2655..2840)
                     /locus_tag="VUW50_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001009512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983822.1"
                     /translation="MNFKEFKTWLDDAVSMAEAMALPENTGVLDDLIENAANNLAFIA
                     ELVENRQLIYRKPRHED"
     gene            complement(2942..3163)
                     /locus_tag="VUW50_001994"
     CDS             complement(2942..3163)
                     /locus_tag="VUW50_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7983823.1"
                     /translation="MELNLRRIKAERIAKGYTQDVIAEKMGWKSRAPYAKRENGIVPF
                     GADELAEFGKILGYGVNELGIFFNKNVPE"
     gene            3343..4083
                     /locus_tag="VUW50_001995"
     CDS             3343..4083
                     /locus_tag="VUW50_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7983824.1"
                     /translation="MRSNSEIIDIIVLEKDKQDLSLSELARRVGLAKSAMSRYLNKTR
                     QFPLNRAQDFANVLGISVEYLLGVENSSPSTSTVEKIMLIADQLTPPRQAKALTYLKK
                     QLLEQKNENIVSENIISLDDYRESKTLPVIGVVTAGNGITQDDNLNIEKCFYTDEIPD
                     DYDAIAYVVGNSMEPKIKNGDYLFIKNTPQVDYNTIGIFQVDGANYVKKLRQGYLESL
                     NPDCADIQLDESNDIRTIGEVVGIYREK"
     gene            4102..4770
                     /locus_tag="VUW50_001996"
     CDS             4102..4770
                     /locus_tag="VUW50_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET7983825.1"
                     /translation="MKFCPECGNSVVGCKFCPNCGYSINASLKTTPQKVATPSSRPST
                     VRSKRTDKVGPLEIDKNHRTYRIHGARKAKGSSGLIGGTAKVVGKTTLALGTGGLSLI
                     PSLIKKDKNDTDWYSFDDLVSYELVINDQSVVSGGVGQALVAGAVFGVIGAVAGGIVA
                     KRKATTKILNMTIRITSNDFTKPVVFIDLIKKPVKNTSKEYKEAIENAQKIIGALDVI
                     THNS"
     gene            5310..5978
                     /locus_tag="VUW50_001997"
     CDS             5310..5978
                     /locus_tag="VUW50_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HET7983826.1"
                     /translation="MTYSQNYLDDILVRMAYHSSGIEGNTISLPETVSIILESTLPRN
                     GKSIREFYEIENHKQAFSYLLDSLANHQALTVGLVQDFHALLVDRLQHDRGQFKQVQN
                     AIIGAEFQTASPAETPYLMTQWADNTAYRLEHAQNEKEVLEILADTHIQIERIHPFSD
                     GNGRTGRLILMYLAMKYLNAPIIISKDSRAHYMELLANQNVTGLADLFKKSLDYERIR
                     KKQF"
     gene            6685..7539
                     /gene="dinD"
                     /locus_tag="VUW50_001998"
     CDS             6685..7539
                     /gene="dinD"
                     /locus_tag="VUW50_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HET7983827.1"
                     /translation="MELQQYDELTFENIKHIDENGVEFWYARDLQKTLEYTEWRNFLL
                     VIDKAKIACDTAKNPIVEHFVDVNKSIPNGYNTTRDIKDFKLTRYACYLIVQNGDPRK
                     EVIALGQTYFALKTRQQELQENFDNLTEEQKRLAIRDEIKHHNKSLSESAGNAGVKNF
                     GRFHNSGYKGLYGGLTMQDIHNLKELNEGEHILDFMGSAELAANLFRATQTDEVLRRR
                     NIKGEDLANDTHFNVGRTIRNTMKELGTTMPENLPAPRESIQELKSKQKELEKQDDNN
                     QLSLFDDM"
     gene            7705..8871
                     /locus_tag="VUW50_001999"
     CDS             7705..8871
                     /locus_tag="VUW50_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET7983828.1"
                     /translation="MKITEYTKKDGSTVYRSSVYLGIDTVTGKKVKTTISGRTKRELK
                     AKALQAQIDFEKGGATVYKAVEIKTYAELVENWLETYCHTVKKSTLMGTKFKIDKYLL
                     PAFGNYRLDKLTPPIIQKQVNQWAKDYNQLGKGFQEYPLLHSLNKRILKYAVSLQAIP
                     FNPARDVIVPRRKEKEGQKLKYLDDDNLKKFLTYLEQLPNTYKNFFDTVLYKTLLATG
                     LRIRECLALEWSDIDLKNGTLDVNKTLNIIKEITGPKTKSSVRMIDLDNKTVLMLRLY
                     KARQSQIGKEMGLTYEKVFSNSFDKHIDARMLSFRLVKHLERAGCPRFTFHAFRHTHA
                     SILLNAGLPYKEIQTRLGHAKLSMTMDIYSHLSKDNQKNATSFYEKAIEKLKSS"
BASE COUNT         2775 a         1841 c         1615 g         2699 t
ORIGIN      
        1 cttttaattg tcctgataag agtcctattc tgaaaaaatc attgttttcg tctgcttggt
       61 gtaattccat attaattcta tccaagcttt tagcgataat atcgtatgtt gtttctatca
      121 tgttttttac ctctgtttct atgttgtgta attgccctaa gggcttttaa tgcttttttc
      181 ctgtcacatg cctcacgctc agaagtttgc cgaccgagag cgtggggctt ttttgagttg
      241 tttcttatac agtttttctt gtaattgcta tcctcacgct cagactcgcc aaattgaaag
      301 cgtgagaaag taccagtttt aagagttggc gctctatgct tttcaaaacc ttttctaatt
      361 gcttgcctgc acttcggttt tcttagtttt tcttcgtttc gtttacggct ttatctgcta
      421 gccgttgcca ttgttttcta aattcttgtt catctatccg cccatgcaga aagtcgctca
      481 ggacgtaagt tgcacggctg taaaatcgct tggctctatc ataatttgtc attctttgcc
      541 tcacttactg cttttactac cttttctctt agttttgtaa attcttcaat aaactcatct
      601 tgagatataa ccccacgatt aaaagttata atcaaggcgc ttgctctttt tgtgtattgt
      661 tccatctgtt acccctccat catgtcataa agtagggcgt aatgtttatc tgggattctg
      721 tccaaggctt tcaagccgtc gtgttctgct ttttgtctgg tttccgcctt gaccgtacta
      781 tcaaaggcga tagaaaaagc attcagcatt gccttgtagc ggtctacgct cttcaaatag
      841 cgcccacgtt ctgacaattc tttatcttcc aattcctctt ttaccgtacc gtccaagagt
      901 gcgaacttgc tataaactcc tttttctagt gtaaaatcta ggcgattgtt ttgtttcagc
      961 ttgcagaggt tggttctgca actctctagg ttacggtaac ctaatacttt agtaatttct
     1021 tctagacgct taccctctaa ttctggcaat ttttcagcta tatctttaaa ttttactact
     1081 tgatgtttct ttcccattgt ttaccttgtc tttctatgct ataatcaaga tatagaaaaa
     1141 atatctatac cctaaatctt gtcgcttgct cgcctcgtct aaaatttgag caagtgattt
     1201 ttttattttc tttttgcatg attactacct gactttggtt tataaagcaa atctttactt
     1261 tcgataagat ccaaaatcca gctgaatccc tgctccaccg tttcaagaaa tgcacctagg
     1321 tcttcactgt ccaataactc gtagttcata caaagatatt cggctagttg tctgtccatc
     1381 tcaactagct ttttaaaatc cttgaaatac ttaggaattt ctactccctt ggcatttgta
     1441 actgtcttaa attcattttc cattttctat actcctatac tttaaaaatt agttctttaa
     1501 tttctgaata tctcatattc aagttgatca tcgctattgc catatcttcc aagcgctgat
     1561 agtttgtcag ttccgcactt gttaaactgt caataccgtt ttcactttct cgctcttgca
     1621 tgagttgggc tttgtttttc ccagtcgctc cctttagcag taggtttgta agagtgctat
     1681 aggcatgctg aggtgctttc tcccatgatt tgatagcttc ggttaaggtc ttgcgctttg
     1741 gcttttccag ttcccgttga agatagcgtt tagaaagttc atcacgcatt tcaaaaaagg
     1801 ctttaaccag gtccttctta aactctttta cgggttctgt atttcgtaag taagtgatta
     1861 acaaagtagc ttgttgctcg ttcaaaatat aatctcgtac attttgccca ctctctgaag
     1921 gtgaaatttt aaattgcacc tttccgaagc tctcaaagtc ctctctgtgc ttattcagca
     1981 aaatctttaa atgtctgtgc ttgatttctg catattctgc aacaatgctg cttaatgtgt
     2041 acggctcttt cttgccgtca acaatacatc atgctctgat gtatttattg caattgtttt
     2101 tgtcatacct tcgtctcact ctctagtgcc ctgagttcaa tctcatgact gacttgaaga
     2161 aatagcttct cacacgctat tttagcttct ctgtacgttg tgttttcgct gatgaagtaa
     2221 tcagcaagtt cgatgatttt atcttccatg accgtctcca aaaatcggtc ttaagaccga
     2281 tgtaacccct tcaaaaacag tatatattta tattatcctt aacaagaaag gagctgatgc
     2341 aaattggcaa aatttttgaa ggggactgtg gttcagtgat tcagtttggc taggtaacca
     2401 acgcgttttt actgcgagtg tgactgcacg gagcctgtcg ctgactataa gagggactgc
     2461 agctctgctt atagcgggac tgacagataa ctaccgagcg acactcaaag actagccaaa
     2521 ccacgttgat tgcagtgctg gacgcatgac cagcgaagtt tcaaccagtt gctttacacc
     2581 gattgtgaaa ccttatcaaa gtatgcaagt cttgacctag tgtaaagtag gttaagactt
     2641 ttttattgtc ttttttaatc ttcatgtcta ggttttctat aaatcagttg acggttttcc
     2701 actaactcag cgataaaagc cagattattg gccgcatttt ctatcaagtc gtcaagtacc
     2761 cctgtatttt caggtaatgc catagcttca gccatactca cggcatcatc tagccatgtt
     2821 ttaaattctt taaagttcat ttcttttctc ctttttttgt tatcaatccc cagtggtaaa
     2881 gcaccagtgg agtatcttgt agatgtaata tagtattgcg attaggttac tccttccgtt
     2941 cttattcggg aacgttttta ttaaaaaaaa ttcccaattc atttacgcca tagcccagaa
     3001 ttttaccaaa ttcagcaagc tcgtcagctc caaatggtac aattccattt tctctttttg
     3061 cgtaaggcgc tcgggacttc catcccattt tttcggctat cacatcttga gtatagcctt
     3121 ttgcaatgcg ctccgcttta attcgtctta ggtttaattc catcagctac cacctttctt
     3181 ttttagtcgt tcccgtaaga gaacaaaaca agtataactc ttccgttccc atttgtcaac
     3241 acaaaacttg atttttttac aaaaaaatat ttttaacatg gttcttgtac ttttttggga
     3301 acggtggtat aataaaatca aattaagaaa ggggggtgta aaatgcgctc taatagtgaa
     3361 attatagata ttatcgtctt agaaaaagac aaacaagacc tttctcttag tgagttagct
     3421 cgaagagtag ggttggcaaa atctgccatg tctagatact taaataaaac cagacaattc
     3481 ccactaaaca gggctcaaga ttttgctaat gttttaggta tatcagtaga atacctgcta
     3541 ggtgttgaaa actcttctcc atcaacttca acagttgaaa aaatcatgct catagctgac
     3601 caactcactc caccaagaca agccaaagcc ttgacttatc ttaaaaagca gctgctggag
     3661 cagaaaaacg aaaacatagt atcagaaaac attatcagtc tggacgacta tagggaaagc
     3721 aagacgctcc ctgttatcgg agttgtaacc gccggaaacg ggataaccca agacgataac
     3781 ctaaacatag aaaaatgctt ttatactgat gaaataccag acgactacga cgctatcgct
     3841 tatgtcgtcg gcaactccat ggagccaaag ataaagaatg gcgactacct atttatcaag
     3901 aatacacctc aagttgacta taacactatc ggtatcttcc aagtagacgg cgctaactac
     3961 gtcaagaaac tgcgtcaagg gtatttagaa agccttaacc ctgattgtgc tgatattcaa
     4021 ctagacgaaa gcaacgatat acgcaccatc ggcgaagtcg tgggcattta tagagaaaag
     4081 taaaaaagga gtaaaaaaat tatgaaattt tgtcctgaat gcggaaattc tgttgtcggt
     4141 tgcaagtttt gtccgaattg cggatactca atcaatgcct cgcttaaaac aactccccaa
     4201 aaagtagcaa ctccatcgtc tagaccttct actgtaagaa gtaaacgtac tgataaagtc
     4261 ggacctcttg aaatagacaa aaatcatcgt acatatagaa tacacggagc aagaaaagca
     4321 aaaggttcat caggtctgat tggcggaact gctaaagttg ttggaaaaac taccttagca
     4381 cttgggacag gcggtttatc tttgattcca tctttaatta aaaaagataa aaatgatacc
     4441 gactggtact ccttcgatga tttagtatct tacgaattag ttataaacga tcagtctgtt
     4501 gtttccggcg gtgttggaca agctttagta gctggagcgg tatttggtgt aataggagcc
     4561 gtagctggcg gaatcgtcgc taaaagaaaa gcaactacca agattttgaa tatgacaatc
     4621 cgaataacat ctaacgattt tactaaacca gttgttttta tagatttaat taaaaaacct
     4681 gttaaaaaca catctaaaga atacaaagaa gctattgaaa atgctcaaaa gattattgga
     4741 gctctagatg ttattaccca taattcataa acaaaactcc ccaacctcat cgaatgcccc
     4801 tctttttcat ggtctattgt gcaaaaacgg ggaaaattga agaatagaaa gccgatttac
     4861 agactaaagc gtaaaattgg gcaaaattgc aacaaacatg aagcgcatcg ctactatttt
     4921 tcttttaggg ctgccgaaag tgccgaaaat gcgacctata gcctaccacg cgccacaatg
     4981 ttcaacaatc ctgaagcgcg tgctttctcg ggcttcagca agtcctctga ccttgtctaa
     5041 acccttctcc tttttagggt gttagaaatg ttagttttcc aaccattaag aactaataga
     5101 aacgggattg gaattgttta ccccatcgct ttttagggac tcagaaaatg aagaaaattg
     5161 tttttttcca gtattcttga aaatccgact ttttccaaca tttatgtgaa gactccgaaa
     5221 aaagcgtggt tgccaagacc taaaaaacaa acaaaacaaa cttaattcca atggaaaaat
     5281 tttagtttaa ttttatagaa aggagaatta tgacctactc acaaaactat ctggacgata
     5341 tactagtacg catggcctac cattctagcg gtattgaagg gaatactatc tctctacccg
     5401 aaaccgtcag cattatctta gaaagcacct tgccacgcaa cggcaaaagt attcgtgagt
     5461 tttatgagat tgaaaaccat aaacaggcct tttcttatct cttggactca ttggcaaacc
     5521 atcaagctct tactgtgggg ctggttcaag attttcacgc cttattagtg gataggctgc
     5581 aacatgatcg cggacaattt aagcaagttc aaaatgccat tatcggggcg gaatttcaaa
     5641 cggctagccc agcagaaacg ccctacttga tgacacaatg ggcggataat actgcttatc
     5701 gtttggagca tgctcagaat gaaaaagaag tattagagat tttagcagac acccatatcc
     5761 agattgaacg gattcaccca tttagcgatg gtaacggtcg tactggtcgc ctgatactta
     5821 tgtacttggc tatgaagtat ctaaacgctc ctatcatcat ctcaaaagac tcaagggcgc
     5881 attatatgga actcctcgca aaccaaaatg taacgggact agctgacctc ttcaaaaagt
     5941 cactggacta cgaaagaata cgaaaaaaac agttttaaaa caacaaaaaa aagacaagct
     6001 agctgctgct gacttgtcta atatcgcctg aactgaaata acgggaaaga ttcaggcgat
     6061 tagtagcatc ttgagagatt ggaagcagaa aaggtctggt tactgttgag ttgggactct
     6121 ttcaaacctc ttagtctacg ctattattat accactcttg catttaaaaa gtgactgacg
     6181 gcatttctca cgctcatcag gctataactc tctaatgaag tataatgcgg tataatttag
     6241 acaattttca acactttggc attttaggaa cgcttaaagc gctctaaaca atatcaaaaa
     6301 aaataaaact cttgaaatat tggggctcgt atgctagaat gaaagcataa agggaaaaac
     6361 agtgcttcta gcatatgtaa ctaaaaagcc ccaccgttac agcgatgggg ttttttgcta
     6421 ccctcttaaa gggttaaaag ctacttgcta cgtctcttta tccataactt gaaaagctca
     6481 gataagacgt taacaagtaa cggagctaga aaaagggaaa acaactctag catatataac
     6541 acctcccttc tctaaattga gggggcgaaa acattatatc aaaaacaccc atcacaaaca
     6601 aactcatatc agcccatatc tactagtttt gttgatgtca acaaatgtca accacttaca
     6661 atattttaga aaggatactt tattatggaa ctccaacagt acgatgaatt aacttttgaa
     6721 aatatcaaac acatagacga aaatggagta gaattttggt acgctcggga cttacaaaag
     6781 acccttgagt atacagaatg gagaaacttt cttctagtaa ttgataaggc aaagatagct
     6841 tgcgataccg ctaaaaaccc cattgtagag cattttgttg acgtcaacaa gtcaattcca
     6901 aacggttata acacaacaag agatatcaaa gattttaaac ttacccgtta cgcttgttac
     6961 cttattgtcc aaaatggtga cccacgaaaa gaagttatag cacttggcca aacctatttc
     7021 gcgttgaaaa ctcgtcaaca agagctacaa gaaaactttg acaatcttac cgaagaacag
     7081 aaacgccttg ctatccgtga cgaaattaaa catcacaata aatcattatc tgagtctgct
     7141 ggcaatgctg gcgttaagaa ctttggaaga tttcacaact caggatacaa ggggttatat
     7201 ggtgggctaa ctatgcagga tattcataac ctcaaagaac taaacgaagg ggagcatatt
     7261 ctggatttta tggggagtgc tgaattagct gctaatttat tccgtgccac tcagaccgac
     7321 gaagttctac gacgtagaaa cataaaaggc gaagatctcg caaatgatac ccatttcaac
     7381 gtaggccgaa ccattagaaa taccatgaaa gaacttggaa caaccatgcc ggagaatctc
     7441 cctgctcctc gtgaaagtat acaagagttg aaaagcaaac agaaagaact tgaaaaacaa
     7501 gatgataata accaactttc gctctttgat gatatgtaat agtgggcaat ataagcccca
     7561 tatccgcctt gttttctact ctggtacaat ttaccgtctg actaattaaa atcgaaaata
     7621 ggggtattct cgtagcccct cgcatggtat aaactcaaaa tcttttctaa ttgcttgcct
     7681 gctgatggaa aaaggagtaa aaccatgaaa ataacagaat acaccaaaaa agacggttct
     7741 acagtctatc gctctagcgt ctaccttggt atcgataccg taacaggtaa gaaagtcaag
     7801 acgactatat cagggcgaac caagagagaa ctcaaagcca aagcactaca ggctcaaata
     7861 gactttgaaa aaggaggggc tacagtatat aaagcggtgg agataaagac ttacgccgaa
     7921 ctggtggaga attggctaga aacgtactgc catacggtta aaaaatcgac cttaatgggt
     7981 accaaattca aaatagataa atatctactt ccagctttcg gaaattatag gctggataaa
     8041 ctgacgcccc caatcattca aaagcaagtc aatcaatggg caaaagacta taatcaacta
     8101 ggcaaaggat tccaagaata tccgctcctt cactctctaa acaaacgcat acttaaatat
     8161 gccgtatctt tgcaagctat cccctttaac cctgcccgtg atgttatcgt gccacgtcgt
     8221 aaggaaaaag aagggcaaaa actgaaatat ctggatgatg ataacttaaa aaaattcttg
     8281 acttacctgg agcagctgcc aaacacttac aaaaatttct ttgatacggt gctatataag
     8341 acacttttag ctactggttt gcgcattcgt gagtgtctag ccttagagtg gtctgatatt
     8401 gacctaaaaa acggcacact agacgtaaat aaaacgttaa atattatcaa agaaatcact
     8461 ggccctaaga ctaaatcaag cgttagaatg attgacttgg ataataagac ggtactcatg
     8521 ctacggctct ataaagcaag acaatcccaa attgggaaag aaatggggtt gacctatgag
     8581 aaagtattct ctaatagctt tgataagcat atagacgcta gaatgcttag tttcaggtta
     8641 gtaaaacatt tggaacgtgc tggatgccct cgttttacgt tccacgcctt ccgccacact
     8701 catgctagta tcttgctcaa tgctggacta ccttacaaag agatacaaac acggctcggt
     8761 catgcaaaac tttctatgac catggatatt tatagccacc tatccaaaga caatcagaaa
     8821 aatgctactt cattttatga aaaagctatt gaaaaattaa aaagttccta aaaaagtcca
     8881 taaaattatt atttcagtta tgggaaatag ctatatcaag gaattttagc
//
LOCUS       DAVDWT010000061         8324 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVDWT010000061 DAVDWT010000000
VERSION     DAVDWT010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8324
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.061"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(<1..752)
                     /locus_tag="VUW50_002000"
     CDS             complement(<1..752)
                     /locus_tag="VUW50_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7983829.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNTPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQI
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHAQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(749..2074)
                     /locus_tag="VUW50_002001"
     CDS             complement(749..2074)
                     /locus_tag="VUW50_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7983830.1"
                     /translation="MDFFLLVDLILYFLIISHSYRLICKDQINRKELFFFGAYTLLTE
                     IVLDFPFYLLYLDGLGIATFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYYGLFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRVYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKRKFE"
     gene            complement(2087..2227)
                     /gene="comC"
                     /locus_tag="VUW50_002002"
     CDS             complement(2087..2227)
                     /gene="comC"
                     /locus_tag="VUW50_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HET7983831.1"
                     /translation="MKNTVKLEQFVALKEKDLQNIQGGEMRKMNEKSFNIFNFFNFFR
                     RR"
     gene            complement(2392..2465)
                     /locus_tag="VUW50_002003"
     tRNA            complement(2392..2465)
                     /locus_tag="VUW50_002003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2429..2431),aa:Arg,seq:tct)
     gene            complement(2509..2988)
                     /gene="rlmH"
                     /locus_tag="VUW50_002004"
     CDS             complement(2509..2988)
                     /gene="rlmH"
                     /locus_tag="VUW50_002004"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET7983832.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            3171..4352
                     /locus_tag="VUW50_002005"
     CDS             3171..4352
                     /locus_tag="VUW50_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET7983833.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            4410..5168
                     /locus_tag="VUW50_002006"
     CDS             4410..5168
                     /locus_tag="VUW50_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET7983834.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNYFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIIYFSNQEEYSRIINSLK"
     gene            5381..6742
                     /gene="dnaA"
                     /locus_tag="VUW50_002007"
     CDS             5381..6742
                     /gene="dnaA"
                     /locus_tag="VUW50_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET7983835.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            6901..8037
                     /gene="dnaN"
                     /locus_tag="VUW50_002008"
     CDS             6901..8037
                     /gene="dnaN"
                     /locus_tag="VUW50_002008"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET7983836.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTIKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            8102..8296
                     /locus_tag="VUW50_002009"
     CDS             8102..8296
                     /locus_tag="VUW50_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HET7983837.1"
                     /translation="MYQVGNFVEMKKPHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
BASE COUNT         2887 a         1473 c         1340 g         2624 t
ORIGIN      
        1 cacttttgag attttttctc taaaatatct tttaattttc taatttttaa tcttgaaata
       61 ggacaacgat ggtcttcata gaaaacaatt tctaagtttt ttcgatcaat ttctctgata
      121 ttgcctatat ttaccaaaaa tgacttgtga ggagaataaa atcgctgagc atgtttgtcc
      181 ttttcctgaa tatctgtcat ggtaccataa aactcttttg caaaattctt accaataatg
      241 cgcaatttat gagagactcc tgtcgtttca atatacaaaa tatcatggta aggaattttt
      301 aaatcatttc ccttgtaatt gtagtcgaaa taatctacaa catcttcatt ttcaagtaac
      361 atactcttcg tgtagaagat attttgctca attcgcttct taaacatctc atcgttgata
      421 tccttatcaa caaaatctag ggctgatatc tggtatttat aggtcagagt cgcaaactct
      481 gatcgactgg tgataaagac gataatagcg taaggattgt aatgacgaat gagttgagca
      541 acttcaaatc cctttttctc aattccatga atatcgatat ctaggaaata aagttgattt
      601 acttcatcat tttcaatgta ttcttcaaat tcacggactt ttcccgttgt cttgtatgat
      661 attggagtat tcgattcttt cgaaatttca tccaatattc tctctagtct cacttgatgt
      721 tcaataacat cttctaaaat taaaactttc attcaaattt cctcttaaat ctaatgattt
      781 gtctaaatgt actgccttcc atctctgttt ctaaaataat gttgttgtac ttatctagta
      841 gttctttcac attatttaat ccgactccgc gatttcttcc cttagtggag aatcctaagg
      901 caaatagatc tcctgaagga gtcatcgtca ttttacatga attctgaatc acaataactg
      961 tttcagtttc catcttaata actgctactt ccatctgctt tttatagcta tcagccgatc
     1021 cttcgacagc attgttcaat aaaacgctca tgatacgcac taaatccaat agttcaattg
     1081 gaagcttggt aatcgtatct tttacttcca gtgtaaactc tacaccatta tttcgagcat
     1141 agacaattga ctgagcaacc aaacttcgta aagctgaatc ttctatgttg ttcaaatcaa
     1201 agtaagtgta cttatctgaa cgtagtttat gatttgcttt gaccaaaact tcattgtaaa
     1261 ctctgtcaat ttcctgtaaa ttaccactgt caattgccat ctgcatgctg acaagcattc
     1321 cagcataatc atgtcgaaaa ccacggattt cattatacag accgacaatt tcatctgtgt
     1381 aattctgtaa atgtttctgt tcaaatttct tctgcttcaa agcaatctct ttctccattt
     1441 gaactttatg agaattcatt gcaaagaagg tcaaaaggag agagataaag acaatagatg
     1501 acaaaatact tccaaaacta ttcaaatgtt taatcgtact taccatatct gaaacgaaag
     1561 atacaatatg tagcaatagt aaagcaaaaa atactttttt caagaaagga taaaggtagt
     1621 ccttgtcaaa ataggctagt tccaaatgga aataataaat gatttttaat gtaacaaaat
     1681 aggtcaacac cgtcacaacg aaaaagaata ggccataata ctgtaaaaca aaattgtctc
     1741 ctgttataga agagaaagtt acggacagaa agttatgagt gctctcatat aaaagagata
     1801 gtagtaaact tagaaatagt cctctatccc tctcatactg tttcatccat cgaaaatagg
     1861 aatataggcc caaaggaaat aaaaatgttg caatccctaa cccatctaaa tatagaagat
     1921 agaagggaaa atcaagtact atttctgtta gtaatgtata agcaccaaaa aagaataatt
     1981 cttttctatt tatttgatct ttacaaatta aacggtaact atgactaata attaaaaaat
     2041 ataatatcaa atctactaat aagaaaaaat ccattctgct actcctttat cttcttctaa
     2101 agaaattaaa gaaattaaag atattaaagg acttttcatt cattttcctc atctccccac
     2161 cttgaatatt ttgcaagtct ttttccttca aagctacaaa ctgttccaat ttaactgtgt
     2221 ttttcataat aaaatctcct aaaatgtttt ttcttgtaag ctaacttaca aaaaccatta
     2281 tacaaaatgg aatttcgttt tagataaaat tctctcaact gtcatttttt tctcccaaag
     2341 tgtacttttt taagaaaaaa gccgggaaaa ttcccagctt tgctattata ttgatcccag
     2401 caggattcga acctgcgacc gttcgcttag aaggcgaatg ctctatccag ctgagctatg
     2461 agacctaaca caattattct accaaaaatt caattaaaag tcaattttct atttatggta
     2521 ggggaatccc tgctgaatcg taaaagcgcg atagatttgt tcaacaagaa ctagtctcat
     2581 taactgatgg ggtaaggtta ggcgaccaaa actgacagaa agattggctc tattttttac
     2641 agatgatgac aatcctaaac ttcccccaat aataaaagta agagtagaaa atccttttat
     2701 agaagtttct tctaactgct tactaaattc ttctgagaag aaagttttcc cttcaatggc
     2761 taacacaata acgaaatcac ggtcagcaat ttttgataaa attctctgac cttctatttc
     2821 taaaatcttt tgattttctg attcactggc cttatctggt gttttttcat ctgataactc
     2881 aatcatttca aacttagcaa atctagaaat tcgttttgaa tactctgcga taccatcttt
     2941 taaatacttt tctttcagtt tcccaactgt tacaacttta attttcatga ctctattcta
     3001 acatattctc tattttttca catcttattc acaaaataaa aaatagattt caattaagaa
     3061 aatcacagtt tcaaaagagt tatccacagt ttgtgtaaaa cttttgtgtt taagttataa
     3121 ttaagctagt cagtttatac tttcagtaat tcaaacatat ggaggcaaat atgaaacatc
     3181 taaaaacatt ttacaaaaaa tggtttcaat tattagtcgt tatcgtcatt agctttttta
     3241 gtggagcctt gggtagtttt tcaataactc aactaactca aaaaagtagt gtaaacaact
     3301 ctaacaacaa tagtactatt acacaaactg cctataaaaa cgaaaattca acaacacagg
     3361 ctgttaacaa agtaaaagat gctgttgttt ctgttattac ttattcggca aacagacaaa
     3421 atagcgtatt tggcaatgat gatactgaca cagattctca gcgaatctct agtgaaggat
     3481 ctggagttat ttataaaaag aatgataaag aagcttacat cgtcaccaac aatcacgtta
     3541 taaatggcgc cagcaaagta gatattcgat tgtcagatgg gactaaagta cctggagaaa
     3601 ttgtcggagc tgacactttc tctgatattg ctgtcgtcaa aatctcttca gaaaaagtga
     3661 caacagtagc tgagtttggt gattctagta agttaactgt aggagaaact gctattgcca
     3721 tcggtagccc gttaggttct gaatatgcaa atactgtcac tcaaggtatc gtatccagtc
     3781 tcaatagaaa tgtatcctta aaatcggaag atggacaagc tatttctaca aaagccatcc
     3841 aaactgatac tgctattaac ccaggtaact ctggcggccc actgatcaat attcaagggc
     3901 aggttatcgg aattacctca agtaaaattg ctacaaatgg aggaacatct gtagaaggtc
     3961 ttggtttcgc aattcctgca aatgatgcta tcaatattat tgaacagtta gaaaaaaacg
     4021 gaaaagtgac gcgtccagct ttgggaatcc agatggttaa tttatctaat gtgagtacaa
     4081 gcgacatcag aagactcaat attccaagta atgttacatc tggtgtagtt gttcgttcgg
     4141 tacaaagtaa tatgcctgcc aatggtcacc ttgaaaaata cgatgtaatt acaaaagtag
     4201 atgacaaaga gattgcttca tcaacagact tacaaagtgc tctttacaac cattctatcg
     4261 gagacaccat taagataacc tactatcgta acgggaaaga agaaactacc tctatcaaac
     4321 ttaacaagag ttcaggtgat ttagaatctt aattgacatc tatgtaaaga aagctttaca
     4381 taagagaaaa gatgtgttag tgtagaatca tggaaaaatt tgaaatgatt tctatcacag
     4441 atatacaaaa aaatccctat caaccccgaa aagaatttga tagagaaaaa ctagatgaac
     4501 tagcacagtc tatcaaagaa aatggggtca ttcaaccgat tattgttcgt caatctcctg
     4561 ttattggtta tgaaatcctt gcaggagaga gacgctatcg ggcttcactt ttagctggtc
     4621 tacggtctat cccagctgtt gttaaacaga tttcagacca agagatgatg gtccagtcca
     4681 ttattgaaaa tttacagaga gaaaatttaa acccaataga agaagcacgc gcctatgaat
     4741 ctctcgtaga gaaaggattc acccatgctg aaattgcaga taagatgggc aagtctcgtc
     4801 catatatcag caactccatt cgtttacttt ccttgccaga acagattctc tcagaagtag
     4861 aaaatggcaa actatcacaa gcccatgcgc gttccctagt tgggttaaat aaggaacaac
     4921 aagactattt ctttcaacgg attatagaag aagatatttc tgtaaggaag ttagaagctc
     4981 ttctgacaga gaaaaaacaa aagaaacagc aaaaaactaa ttatttcata caaaatgaag
     5041 aaaaacagtt aagaaaacta ctcggattag atgtagaaat taaactatct aaaaaagaca
     5101 gtggaaaaat cattatttat ttttcaaatc aagaagaata tagtagaatt atcaacagcc
     5161 tgaaataagg ctgttctttt atttttttat ctcacaaggt tatccactat gtttttcgat
     5221 aaaaagctta ataaatcaat aatttcttct tttatcccca acctgtggat aaagtttagt
     5281 aacattgtgg attatttttc acagcttgtg gaaaattctt gctatctatg gtaaaatatc
     5341 tctagtatta aacttttaaa tagtaaagga ggagaaagga ttgaaagaaa aacaattttg
     5401 gaatcgtata ttagaatttg cacaagaaag actgactcga tccatgtatg atttctatgc
     5461 tattcaagct gaactcatca aggtagagga aaatgttgcc actatatttc tacctcgctc
     5521 tgaaatggaa atggtctggg aaaaacaact aaaagatatt attgtagtag ctggttttga
     5581 aatttatgac gctgaaataa ctccccacta tattttcacc aaacctcaag atacgactag
     5641 ctcacaagtt gaagaagcta caaatttaac tctttatgac tatagtccaa agttagtatc
     5701 tattccttat tcagatacgg gattaaaaga aaagtatacc tttgataact ttattcaagg
     5761 ggatggaaat gtttgggctg tgtcagccgc tttagctgtc tctgaagatt tggctctgac
     5821 ctataaccct ctttttatct atggaggacc aggccttggt aagactcact tattaaacgc
     5881 tattggaaat gaaattctaa aaaatattcc taatgcgcgt gttaaatata tccctgccga
     5941 aagctttatt aatgactttc ttgatcacct aagacttggg gaaatggaaa agtttaaaaa
     6001 gacctatcgt agtcttgatc ttttgttaat cgatgatatc cagtcactca gcggaaaaaa
     6061 agtcgcaact caggaagaat ttttcaatac ctttaacgcc cttcatgaca agcaaaaaca
     6121 gattgtccta acgagtgatc gtagtccaaa acatctagaa gggctcgagg agaggcttgt
     6181 cacgcgtttt agttggggat tgacacaaac tatcacaccc cctgactttg aaacacgtat
     6241 tgccatttta caaagtaaaa cggaacattt aggctacaat ttccaaagtg atactctaga
     6301 atacctagct gggcaatttg attcaaatgt tcgagatctt gagggagcca tcaacgacat
     6361 cactttaatt gccagagtaa aaaaaatcaa ggatatcact attgatattg ctgcagaagc
     6421 cattagagcc cgcaaacaag atgttagcca aatgctcgtc atcccaattg ataaaatcca
     6481 aactgaagtt ggtaactttt atggtgttag tatcaaagaa atgaagggaa gtagacgcct
     6541 tcaaaatatt gttttggccc gtcaagtagc catgtattta tctagagaac taacagataa
     6601 tagtcttcca aaaattggga aggaatttgg gggaaaagat cataccacag tcattcatgc
     6661 ccatgccaaa ataaaatctt tgattgatca agacgataat ttacgtttag aaattgaatc
     6721 aatcaaaaag aaaatcaaat aatttgtgga taacttttag ttttttatct tttttatcca
     6781 cattttttaa acaagctaaa aaacttgata tgacttgttt aaaggctgtt ttccacagat
     6841 ttcacagact ctattattac tattatcttt ctaatactaa aaataaataa aggagaatcc
     6901 atgattcatt tttcaattaa taaaaattta tttctacaag cattaaatac tactaagaga
     6961 gctattagtt ctaaaaatgc cattcctatt ttatcaacaa taaaaattga cgtgaccaat
     7021 gaaggtatta ctttaattgg ttcaaatggt caaatttcaa ttgaaaattt tatttctcaa
     7081 aaaaatgaag atgctggttt gttaattact tctttaggtt cgatccttct tgaagcttct
     7141 ttctttatca atgtagtatc tagtttacct gatgtaactc ttgattttaa agaaattgaa
     7201 caaaatcaaa ttgttttaac cagtggcaaa tcagaaatta ccctaaaagg aaaagatagc
     7261 gaacaatatc cacgaatcca agaaatttca gcaagcactc ctttaatact tgaaacaaaa
     7321 ttactcaaga aaattattaa tgaaacagcc tttgctgcaa gtacacaaga gagtcgtccg
     7381 attttaacag gtgtccactt cgtattgagt caacacaaag agttaaaaac agttgcaaca
     7441 gactctcatc gcctaagcca gaaaaaattg actcttgaaa aaaatagtga tgattttgat
     7501 gtcgtaattc ctagccgttc tctacgcgaa ttttcagcgg tatttacaga tgatatcgaa
     7561 actgtagaga ttttctttgc caataaccaa atcctcttta gaagcgaaaa tattagcttc
     7621 tatactcgtc tcctagaagg aaactatcct gatacagatc gcttgattcc aacagacttt
     7681 aacactacta ttacttttaa tgtggtaaac ttacgccagt caatggagcg tgcccgtctt
     7741 ttatcaagtg cgactcaaaa tggtactgtg aaacttgaaa ttaaggatgg ggttgttagc
     7801 gcccatgttc actctccaga agttggtaaa gtaaacgaag aaatcgatac tgatcaggtt
     7861 actggtgaag atttgaccat tagtttcaac ccaacttact tgattgattc tcttaaagct
     7921 ttaaatagcg aaaaggtgac cattagcttt atctcagctg ttcgtccatt tactcttgtg
     7981 ccagcagata ctgacgaaga cttcatgcag ctcattacac cagttcgtac aaattaagtg
     8041 aaagaggttg agcctggctc gcctctttta tgatataatc gaaaaagaaa aggagagtag
     8101 tatgtatcaa gttggaaatt ttgttgagat gaaaaaacca cacgcttgta caatcaagtc
     8161 gactggtaaa aaggctaatc gttgggaaat tacacgtgta ggagcagata tcaaaataaa
     8221 atgcagtaat tgtgagcatg ttgtcatgat ggggcgatat gattttgagc gaaaaatgaa
     8281 taaaattatt gactgagaac ccttagttag agggttagca cttt
//
LOCUS       DAVDWT010000062         7953 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVDWT010000062 DAVDWT010000000
VERSION     DAVDWT010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.062"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(<1..278)
                     /locus_tag="VUW50_002010"
     CDS             complement(<1..278)
                     /locus_tag="VUW50_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET7983838.1"
                     /translation="MYEEKQYFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFANTYI"
     gene            complement(299..796)
                     /locus_tag="VUW50_002011"
     CDS             complement(299..796)
                     /locus_tag="VUW50_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET7983839.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(869..2230)
                     /gene="radA"
                     /locus_tag="VUW50_002012"
     CDS             complement(869..2230)
                     /gene="radA"
                     /locus_tag="VUW50_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET7983840.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYISGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(2244..2759)
                     /locus_tag="VUW50_002013"
     CDS             complement(2244..2759)
                     /locus_tag="VUW50_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7983841.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     TPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(2761..3204)
                     /locus_tag="VUW50_002014"
     CDS             complement(2761..3204)
                     /locus_tag="VUW50_002014"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET7983842.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(3264..3357)
                     /gene="ffs"
                     /locus_tag="VUW50_002015"
     ncRNA           complement(3264..3357)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VUW50_002015"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3390..3857)
                     /locus_tag="VUW50_002016"
     CDS             complement(3390..3857)
                     /locus_tag="VUW50_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET7983843.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(4058..5344)
                     /locus_tag="VUW50_002017"
     CDS             complement(4058..5344)
                     /locus_tag="VUW50_002017"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET7983844.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(5575..5811)
                     /locus_tag="VUW50_002018"
     CDS             complement(5575..5811)
                     /locus_tag="VUW50_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HET7983845.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            6077..6888
                     /locus_tag="VUW50_002019"
                     /pseudo
     CDS             6077..6888
                     /locus_tag="VUW50_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6923..7769)
                     /locus_tag="VUW50_002020"
     CDS             complement(join(6923..7451,7450..7769))
                     /locus_tag="VUW50_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET7983846.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQISRNTIYGWLKLKEK
                     TGELNHQVKGTKPRKVDRDRLKNYLTDNPDAYLTEIASEFGCHPTTIHYALKAMGYTR
                     KKEPHTYYEQDPEKVALFLKNFNSLKHLAPVYIDETGFDTYFYREYGRSLKGQLIRGK
                     VSGRRYQRISLVAGLTNGELIAPMTYEEMVTSDFFEAWFQKFLLPTLTTPSVIIMDNA
                     RFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAYIKKNLKKVLPSCNTFYEALF
                     SCSCFN"
     gene            complement(7877..7950)
                     /locus_tag="VUW50_002021"
     tRNA            complement(7877..7950)
                     /locus_tag="VUW50_002021"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7914..7916),aa:Asn,seq:gtt)
BASE COUNT         2510 a         1873 c         1271 g         2299 t
ORIGIN      
        1 tggatataag tattagcaaa actagacaac cagcctgaaa ggaacatccc tcccaataaa
       61 gacagaatca aaaccttctt tggcaattcc cattttttcc caatacggaa gagaaaataa
      121 agagccggaa tcatgtaaaa gagagctaga aagatagaaa ctcccattag tccatattcc
      181 gcacctgacc tcgaaccgtc cgtatagtag atggtttcat actgtaaacc aatacatagc
      241 aataaaataa aaataaaata ctgcttttct tcatacactt tctttctaaa tgaagtattt
      301 ataattctac gactgtcata cttcctgtat caacattgta aatagcacca gagataatga
      361 catcgtctgg tattagggga gactcgataa gcagttgcat atcctcgcgt acactctctt
      421 ctatatcttg gaagggcaag aagtcctggt ctgacacatc tacacctaat tcctctttta
      481 aatactcctg aaaaggttca ttttcaaagg tctgagcacc acagtctgta tggtgcaata
      541 ccacaatctc tcttgtcccc atttgttgct gggaaataac tagcgaacga atcatgtctt
      601 cagtcactcg accacctgca ttccgcaaga tatgagcatc cccaagtgcc aaacctagag
      661 cttgcgcaac gtgcaaacgt gagtccatac aggtcacaat agctactctt gttttgggtt
      721 taagtggcag atttaactgc ccatgtaggg caacataagc ctgattagct tgcataaact
      781 gttcaaaata cgacacgatt ccctccttga aaatttgata gtcaaatatt tctcctatct
      841 tatcattttt aagagaattt gtcacggatt atgcaaagac ctttttcaaa acttcctgaa
      901 tcgttgtcac gccaatgacc tgaatttcct taggcagagt gattcctgtc aaggaattct
      961 taggtacata aatcttagta aagcccagtt tagcagcttc gttgatgcgt tgctcaatac
     1021 gattcacgcg ccgaatctct cctgtcaagc ccagttctcc gacaaaacat tcctgaggat
     1081 tagttggctt gtctttgtag ctcgaagcaa tagcaactgc aacagccaag tcaatggcag
     1141 gttcatccaa tttaacacca ccagcagatt tgagataggc atcctgattt tgcaagagaa
     1201 gccctgcccg tttttccaaa acagccataa tcaagctagc acggttaaaa tcaagtcctg
     1261 tcgtagtacg cttggcattt ccaaacatgg tcggtgttac caaagcctga acctccgcca
     1321 aaatcggacg cgtcccttcc atggttacaa cgatggagga accagttgcc ccatccaaac
     1381 gctcttctag gaaaacttga ctcggattga gtacctcaac caagccgccc gactgcatct
     1441 caaaaatccc aatctcatta gtggaaccaa aacgattttt gaccgctctc aaaatacgaa
     1501 aggtgtggtg acgctcccct tcaaagtaaa gcaccgtatc caccatatgc tccaacatac
     1561 gaggcccagc caaggttcct tctttggtca catgacctac gataaagatg gcaatgttat
     1621 tggtcttggc caactgcatg agttcagcgg tcacttcacg tacctgagaa acagacccct
     1681 gcacccctga aatctcagga gacatgatgg tctggatgga atcaataata agaaagtctg
     1741 gctggatacg ctccacttct gcacgaacac tctgcatatt ggtctctgca tagagataaa
     1801 actcactatc aatatcacct aagcgctctg cacgtagttt aatctgctgg gcagactcct
     1861 ccccactgat atagagaact gtccccactt gggacaactg ggttgagact tgtaggagaa
     1921 gagttgattt cccaatccca ggatccccac cgatgaggac gagacttcct ggtaccactc
     1981 cgcctccaag cacacggttg aattcctcca tctccgtctt ggttcgattg acattgatgg
     2041 aagtcacctc agctagtttc atgggcttgg ttttctcacc tgtcaaggac acacgcgcat
     2101 tcttgacctc ggcaacctca acctcttcca caaaagaaga ccaagaccca cagttggggc
     2161 aacgtcccag atatttaggg gaattatacc cacaattttg acatacaaat gtcgcttttt
     2221 tctttgcgat gacaaacctc tttctatatc tctaactcac actcaatcac ttggcaaaaa
     2281 tcaatcttct catttggcac aaactggcgc atgagcattc gatgagcaac aactaccaca
     2341 gtctgatgtt ctcgatactt agacatacat tctagaaacc gagacttcat ttccgtagct
     2401 gtctcatatt gaataggact attaggaagc aactccccct tgttttctaa aaacagtctt
     2461 ctagctgttt caaagttttc tattcctgtt ttatagacct gccattcatg taataaaggc
     2521 tctactctta aaggaagacc cgtagcacag accacatacg aagccgtttc taaagctctt
     2581 gtgactgcag aagatacgat tatttcagct gacgagagta aaggagtttt gctcaatttc
     2641 tggacttgct gccgtcccat ctcagacaag ggtgccaaat ctatcccaaa tcctatataa
     2701 gaacgctcct ctaactcacg gtaatctggc tccccatgac gtacaaagat aatcttcatt
     2761 ctagtgccct gtcgatccaa atccaccagt tcgaacgcca tcagctgcat ctccatctgc
     2821 aattaagaaa gtagcaaaaa cagcctggac aatacgttcc ccaacttcaa gaacaacctc
     2881 ttggtctgtg atattcttca tctgcgcaaa aatatgccct tcatttccag gatttccata
     2941 ataatcccca tcaatgaccc caactgagtt aattaaaacc aagcccttct tacgaggatt
     3001 tgaagaacga tcatagaggt agagaacctc agtcggctgc atataagcct taacccctgt
     3061 cggaaccaag acaatctctc ctggcgcaac aactgtacgc acagcaacct ttaagtcgta
     3121 accagccgca tgcgctgtct cacgcttggg caataaattt tcatctgtaa aactcgaaac
     3181 caattcaaaa ccacgaattt tcataatttt ctcttttcta ttatcattta ttctagatta
     3241 ttctatctta tttattcgga aaaagcacga aaaaagagca cacaattcaa atcgcttagg
     3301 gctgctggat tcctcccctg acccgcttca cgcagaactg ttgctccact atttattata
     3361 tcacattcct attcatttta aaagcaaaat tattttttcc gtctatttct aaaaaaatcc
     3421 tgcatgatag ctgcgcattc atcttccaaa attcctattt caacctccac acgatgattg
     3481 agacgctcat ctgtcaagat attgtacaaa ctcccagcag cgccaaattt ctggttttta
     3541 gccccataga ccacatttgg aatgcgggca agcccaatcg ctccactaca catgacacaa
     3601 ggttcaatgg tcacaaaaag tgtgcaatcc agcaagcgcc agctctcctc actcaagttc
     3661 gcatcctcta tagccataat ttccgcatgc ataaccgctc gctgtaattc ctcacgcgca
     3721 ttatgcccac gaccaatgat ttccccatct ttgacaatca cacaaccaat tggaatttca
     3781 tcgtgttcaa gagcaatctc agcctctctc aaagcctccc tcataaagac ttctttttct
     3841 tcaagcgtat aatacatctc ttttctcttt tcctacttat cgattttatt attatatcat
     3901 gaatcccaac acaaaagaag ccaccgaatg cggtgactct atagggagat tattatgaaa
     3961 aagaaaagtt taggatattt gttacaacaa gttaggaggt cttcttgtaa ccgtctatag
     4021 tatacccgac ctatcttaaa caaatcttaa aaatctctta ggaccaaaca ctttctaaaa
     4081 tatttgtttg ttcacgacca ggacctactg agaaagtaga aatacgaacg ccaaccaatt
     4141 cactcacacg acgaacatag ttacgcgcat tctcaggaag atcttccaaa ttgcgaactc
     4201 cggtaatatc ttctgaccaa cctggcaact cttcatagat aggcttgcaa cgtttcaatt
     4261 gctcaagact agctggatag tagtcaatac gttgaccgtc aagatcatag gccacacaga
     4321 ttttcacagt atccaaaccg ctcaaaacat caatagagtt caaagaaagg ttagtaatac
     4381 cagaaacacg acggctatga cgcatcacaa ctgagtcaaa ccaacctaca cgacgtggac
     4441 gaccagttgt tgtaccatat tcatgaccca cttcacggat acgttctccc acttcatcaa
     4501 acaactcagt tgggaaagga ccatctccta cacgactcgt ataagcttta catacaccta
     4561 caaccttgtc aatcttgctt ggaccgacac cagaaccaat tgtcacacca ccagctacag
     4621 ggtttgatga cgtaacaaat ggataagtac cttggtcgat atctagcata acaccttgtg
     4681 caccttcaaa aagcacacgt ttgccattat caagcgcatc attcaagata acagatgtat
     4741 ctatcacgta tttcttgatt tgttgaccat attcgtaata ttcttcaaaa atatcatcga
     4801 aaacaatcgc tttactgtca tacaattttt caaaaagacg attcttttca gcaaggttac
     4861 gttctaaacg ctcacggaaa atatctttat ctaaaagatc tgcaatacga attccaacac
     4921 gagcagcctt gtccatataa gctggaccaa ttcccttaat tgtcgtacca atcttattgt
     4981 cgcccttagc ttcttcttgc aagcgatcca actcgatatg ataaggcaaa ataacatgcg
     5041 cacgatcaga aatacgcaag ttatcagttg ttacaccttc ctcatgaaga tagctcaact
     5101 cttttacaag agatttagga tttacaacca taccattccc aatgacagat attttttcag
     5161 ggaagaaaat cccagatgga atcaagtgca acttaaattt cttaccgtca atcacaatcg
     5221 tgtgaccagc attatcacca ccttggtaac gtgcaattac ttctgcattc gctgaaagga
     5281 agtctgtaat cttcccttta ccttcatcac cccattgggt acctacaaca acaactgaag
     5341 tcataatttt gtctgagccc tcaggctctt cctttctcac atacatggca ggactctcac
     5401 ctgcaattat atcttacaat ttattataat aaaaaatcgc ctttttatca agaagaaaca
     5461 atagaaagat ttgctatttc caactattaa aaaatgattt agaaaaatta ctagctattt
     5521 actattatct ttccataaaa gagtaaatta gttcggaaat ttactaaaat tacctcaaca
     5581 agaaataaac ccccgattca ttaccaattt ttcaagatac aaacgataag caacacgata
     5641 atggtaaacg ataaaatccc tacgacaacc aatgccatat ctcactaaat aataaattaa
     5701 aaatttaaaa tgaacatgtt cccagtcaaa attatcacca aatgtaggac catactcttc
     5761 ttcaatacta tcatagaaat tagccatctg ctcataaatt ttttgtaaca taatcaaata
     5821 ctccttttct tttttataaa cttattctaa caaaaaattt tacacattca ctatcaattc
     5881 ctgaattgtt aaaataccag cctcaacaag ataaaaatag gaaaagttga caaaatagaa
     5941 acaaatttgc tacctaaatt tcaaacaatt aaagttctaa aaagaccgcc ttaaaacttt
     6001 tgggtaaatc ctattaaaac tataatattt ttatataggt attatggttc tgattttcta
     6061 gtataaaaaa attcaaacta gaattctcct tagaaagcgt cgattcaagt gctatcactg
     6121 ttcaaaaatg atggtcgctg aaacttctat cgtcaagaag aatcaccaaa tccctcgtat
     6181 tatcaaccaa aagattgctc aaaagttaat tgaaaagatt tctatgaccg acattgatca
     6241 tcagttgtct atttcaactt caactgtcat tcgcaagatc aatgattttc actttgagca
     6301 tgatttttcg cgtcttcctg agattatgtc ctaggacgtt gaaacagtct ggggagtgac
     6361 tgtttcaatc gggagataga gatgagcttt attgctcagg actttgataa gctcaatatc
     6421 ataactgttc ttgagagcag aacacaagcc atcatccgaa atcccatgaa tacaaggcta
     6481 tcaagcgcta ctggagctca ttcaacaaga tagtcagaaa ctgagtgata aacgatttta
     6541 tcgccctact tttcgtatgc acttgactaa taaagaaatt ctagataaac ttttgagcta
     6601 ttcgaagact tgaaacacca ctataatctc tatcagctat aatctctatc agctcttgct
     6661 tttccacctc tttttcagac tctgtttaaa accttcctca aagataaaga gaaaatcgtc
     6721 aacgcccttc aattacctta ttccaacgcc aaactggaag ccaccaataa tctcatcaaa
     6781 cttatcaagc acaatgcctt tggttttagg aactttgaaa acttcaaaaa agaaaggacg
     6841 aaatttgtcc tttctagatc ttcgctttct tcaacccact acagttgaca aagaaccgaa
     6901 aaatgtctcg cctctaatat agtcaattga aacaagaaca agagaaaaga gcctcgtaaa
     6961 aggtattgca acttggtaat acctttttga ggttcttttt gatataagcc catgttttct
     7021 caataggatt gtactcaggt gagtagggag gaagaggtaa aagtttatgc ccaaactcct
     7081 cgcataaaag ttctaactta cccattctat ggaatcttgc attatccata ataataaccg
     7141 atggtgtggt taatgttggt aagagaaact tctgaaacca agcttcaaaa aagtcgctcg
     7201 tcaccatctc ttcgtaagtc attggagcga ttaactcacc atttgttaga cctgcaacca
     7261 aagaaatcct ctgatatctt cttccagata ctttacctct tattaactga ccttttaatg
     7321 agcgaccata ttctcgataa aaataagtat cgaatcctgt ttcatcaata taaacaggtg
     7381 ctaggtgctt taaactatta aaatttttaa gaaataaggc tactttttct gggtcttgtt
     7441 catagtaggt gtggttcttt ttttcgagtg tagcccatag ctttgagcgc atagtggatg
     7501 gtagttggat gacagccaaa ttcagaagct atttcagtca aataagcgtc tggattgtca
     7561 gtaagatagt ttttaagtct atctctatca acctttcttg gttttgttcc ttttacttgg
     7621 tggtttagct ctcctgtttt ctcttttagc tttaaccagc cataaatggt attacgtgag
     7681 atttggaaaa cgtgtgacgc ttctgttata ctacctgttc gctcacaata agagagaact
     7741 tttttacgaa aatctattga atatgccata aaaagattat accacattgt gtactatttt
     7801 tggttcattt tactataaca caaaatagat tattattaca taacaaaaaa gaggtctaaa
     7861 cctcttaact caattactcc gccagtagga ctcgaaccta cgacatcatg attaacagtc
     7921 atgcgctact accaactgag ctatggcgga tta
//
LOCUS       DAVDWT010000063         7246 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVDWT010000063 DAVDWT010000000
VERSION     DAVDWT010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.063"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(64..1026)
                     /gene="nrdF"
                     /locus_tag="VUW50_002022"
     CDS             complement(64..1026)
                     /gene="nrdF"
                     /locus_tag="VUW50_002022"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HET7983847.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKTEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSADD
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(1214..3373)
                     /gene="nrdE"
                     /locus_tag="VUW50_002023"
     CDS             complement(1214..3373)
                     /gene="nrdE"
                     /locus_tag="VUW50_002023"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HET7983848.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(3454..3672)
                     /gene="nrdH"
                     /locus_tag="VUW50_002024"
     CDS             complement(3454..3672)
                     /gene="nrdH"
                     /locus_tag="VUW50_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HET7983849.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            4023..4286
                     /locus_tag="VUW50_002025"
     CDS             4023..4286
                     /locus_tag="VUW50_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HET7983850.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            4292..6025
                     /gene="ptsP"
                     /locus_tag="VUW50_002026"
     CDS             4292..6025
                     /gene="ptsP"
                     /locus_tag="VUW50_002026"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET7983851.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            6280..>7246
                     /gene="phtA"
                     /locus_tag="VUW50_002027"
     CDS             6280..>7246
                     /gene="phtA"
                     /locus_tag="VUW50_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HET7983852.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLIFDPAQITSRTAR"
BASE COUNT         2232 a         1346 c         1556 g         2112 t
ORIGIN      
        1 ttttgttggg ttataacttt gatgtttcta gtttactttt tgatgattga gagtgatgga
       61 gaattagtct aaaccgtagt tatagtcatc gtcttgcatg gcttcaactt cgccaagaag
      121 gtaaccattt ccgacttgag agaagaagtc gtggttggaa gttcctgttg aaataccgtt
      181 cattacgatt gggttgacat catcagctga atctgggaag agtggatctt gtcccatgtt
      241 catgagagct ttattggcat tgtagcgaag gaaggtttta acctcttcag tccaaccaac
      301 accgtcataa agactctctg tgtagccttc ttcattttca taaagagtat agagtaggtc
      361 gtacatccat tctttgagtt tttcttgctc ttcttcaggt aattcattga aaccaagttg
      421 gaatttgtaa ccaatgtagg ttccgtgaac agactcgtca cgaataatca atttaatgat
      481 ttctgcaacg ttggcaagtt tgttgttacc gagatagtag aggggagtga agaaaccaga
      541 gtagaagagg aaggtttcga ggaagacgct ggcaactttc ttttcaagtg ggctgccgtt
      601 taggtagatt tcgttgacaa tctcagcctt cttttgtagg taaggattgg tattggtcca
      661 ttcgaaaatt tcttcaatct cagtcttagt attcaaggta gaaaagattg atgagtaaga
      721 tttagcgtgg acagattcca taaattggat gttattgaaa acagcttcct catgtggtgt
      781 acggatgtct gcgcgaaggg cttgaacccc agtttcagat tgcatagtgt caagaagggt
      841 taaaccacca aaaacttttc cgaccaagtc tttctctttg ttagatagct ttctccagtc
      901 atccaagtcg tttgataagg gaatacgtgt atcgagccaa aattgctccg tcagtttttc
      961 ccaagttgat ttgtcgatga catcttcgat ggcattccag ttaatggctt tgtagtaagt
     1021 ttccatttaa aatctctttc tgtgtttagt attgcgaact cacaattatt tctactttac
     1081 cataattcta taggagtatc gcacaaaaag tcggaagccc gacttttaaa atgttacata
     1141 aattatgtta tgacatagta gatttgattt tatcagtgct gcttagggaa aaataatgtt
     1201 tctatgctag aaactaaatc acacagcttt cacattggtt ggcgccgact tctccaccgt
     1261 catctgtaaa ggtacggacg tagtagatag acttgattcc cttgttaaag gcatagttac
     1321 gaaggatgga caagtcacgt gtcgtttgtt tattttccct cttccattcg taaaggcctt
     1381 ttggaatgtc actacgcatg aagagggtga gtgaaagtcc ttgatccacg tgttcagtcg
     1441 cagcagcgta aacatcgatg actttacgca tatccatatc gtaggcagaa gtgtagtaag
     1501 gaatggtttc tgtagacaag ccagcagcag ggtagtagat tttaccaatt ttcttctctt
     1561 ggcgttcttc gatacgttgc gtaatcgggt ggatagaagc agaaacgtcg ttgatatagc
     1621 tgatagaacc atttggcgct acagcaaggc gattttggtg gtaaagacca tcttcttgaa
     1681 ccttgtcgcg aagttcagcc caatcagcaa caccagggat aaagacattt ttgaagagtt
     1741 ctttaacacg gtctgatgtt ggaacaaatt caccagttac atacttgtca aagtaacttc
     1801 cgttagcata gtctgatttt tcaaagttgt ggaaggtaat accacgttca cgcgcaatat
     1861 tgtttgactc taccaaggtc cagtagttca taagcataaa gtagatgctt gtaaattcaa
     1921 cagactcagg tgaaccatat tcaatgagtt gttgggcaag gtagctgtgc agtcccatgg
     1981 caccgagacc aaaggtgtgg gcttggctat ttccatggtc aatcgttggt acagctacga
     2041 tatgtgaact atctgtaacg aaagtaaggg cacgaaccat agcacggata gaacgaccaa
     2101 aatcaggtga agtcatcatg ttaaccacgt tggttgaacc caggttacat gaaacatctg
     2161 ttcccatttg aaggaattct tgagcatcgt tgatcaagct tggttcttga acttgaagaa
     2221 tctcagaaca caagttactc atgataatct ttccatcaac aggatttgca cggttagccg
     2281 tatcgatgtt gactacataa ggatagccag actcttgttg caatttagag atttcagttt
     2341 ccaaatcccg cgccttgatt tttgtcttgc gaatatttgg atttgcgacc aattcatcgt
     2401 atttttcagt aatgtcgatg taattgaatg gcacaccgta ttctttttct acagagtaag
     2461 ggctgaagag gtacatttct tcatttttac gagccaattc gtagaattta tcaggtacta
     2521 caacaccaag tgatagagtc ttgacacgta ctttttcatc agcgttttct ttcttagttg
     2581 aaaggaaagc gatgatatct gggtgaaaga cgttgaggta gacaacaccg gcaccttgac
     2641 gttgccccaa ttggttggag taagagaagc tgtcttcaaa aagcttcata acaggaacga
     2701 cacctgaagc agctccttca tagcctttga taggtgcacc agcttcacga aggttgctga
     2761 gggtaattcc cacaccacca ccaatacgtg aaagttgaag agctgagttg atagaacgtc
     2821 cgatagagtt catatcatcc gtcacttgga ttaggaaaca agataccaac tctccacggc
     2881 gagcacgtcc agcattcaag aaggaaggag tagcaggttg gtagcgttgg tggatgattt
     2941 cattggcaat atcgattgca acagcttcat tcccatcagc gaaataaagg gcgttgaaga
     3001 agacacggtc ttccatattt tcaagatagt attcaccgtc attagtcttt aaggcatatt
     3061 gattgtaaaa tttataagct gccatgaatg acttgaattg gaagttttgg tctttgataa
     3121 attgagctaa ttcttccaag aactctggac ggtatttctt gataaaggct gtttcgatgt
     3181 agttgtgttc aatgaggtaa ttgattttgt ctttgattga atcaaaaacc atagtgtttg
     3241 gaactacatt ttctttaaag aaagcatcca aggcttcctt gtctttatga agcatgattt
     3301 gtccattaac aggacggtta atttcgttat taagacggaa gtaagtcacg tcttcaagat
     3361 gttttaatcc cataaaattt cccttatcta attacaaaag aaaggcttct aagttagccc
     3421 taaaagcagt ttcttctgga tgatgtacta agattatgct aattgtttca gttttcctgg
     3481 ttggaaacct gaaaagactt cagttggtgt ttggataaca ggagctgcgc taaaaccgag
     3541 ctctttaact tgatcgacgt actcaggttg ctcatcaaga ttgatttcac gataagagac
     3601 attattactg tccaagaaac gcttggtcat tttacattgg acacaattgt ttttagaata
     3661 aacggttacc attgtgtaac tcctcttcaa aatttaatac tatcttagta tatcagaaaa
     3721 taaaattttg tcaatgattt gaactaaata tagtggtgtt cttttgttgt ttttatatat
     3781 aaacacaata tatagtgttt gtattgtgaa atagcaatga ttatcctata aattgttttt
     3841 aaagaaacta tatcctgtgt tttgttatct aggatagaag attttcagca agttatctga
     3901 aaggaaattg aaggaaaatc cagaaaatgc ttgaaaaaaa tcctagaaga tggtataata
     3961 ctaaattgta agggttatca catataactc aaaaaaagaa agaacaaaag gagagtcaaa
     4021 ctatggcttc taaagatttc cacgtagtgg cagaaacagg tattcacgca cgtccagcaa
     4081 cattgttggt acaaactgct agcaaatttg cttcagatat cactcttgag tacaaaggta
     4141 aatcagttaa ccttaaatca attatgggtg ttatgagtct tggtgttggc caaggtgctg
     4201 acgtaactat ctcagctgaa ggtgcagatg cagatgacgc tatcgctgca atctcagaaa
     4261 caatggaaaa agaaggattg gcataaggga aatgacagaa atgcttaaag gaatcgcagc
     4321 atctgacggt gttgcagttg caaaagcata tctactcgtt cagccggatt tgtcatttga
     4381 gactattaca gtcgaagata caaacgcaga agaagctcgc cttgatgccg ctctacaggc
     4441 atcacaagac gagctttctg ttattcgcga gaaagcagta ggtacgctcg gtgaagaagc
     4501 agctcaagtt tttgatgctc acttaatggt tcttgctgac ccagaaatga tcagccaaat
     4561 caaggaaact atccgtgcga agaaagtgaa tgcagaagca ggtctgaaag aagttacaga
     4621 tatgtttatc actatctttg aaggcatgga agacaaccca tacatgcaag aacgcgcagc
     4681 ggatatccgc gacgtgacaa aacgtgtatt ggcaaacctt cttggtaaaa aattgccaaa
     4741 cccagcttct atcaatgaag aagtgattgt gattgcgcat gacttgactc cttcagatac
     4801 agctcaattg gacaaaaact ttgtaaaagc ttttgtaacc aacattggtg gacgtacaag
     4861 ccactcagct atcatggcac gtacacttga aattgctgct gtattaggta caaacaacat
     4921 cactgaaatc gttaaagacg gtgacatcct tgctgttaac gggatcactg gagaagtgat
     4981 tatcaaccca acagatgaac aagcggcaga atttaaagca gctggtgaag cctatgcgaa
     5041 acaaaaagct gaatgggcac ttttgaaaga tgctcaaaca gtgactgctg acggtaaaca
     5101 cttcgagttg gctgccaata ttggtactcc aaaagacgtt gaaggtgtta acaacaacgg
     5161 tgcagaagct gttggacttt accgtacaga gttcttgtac atggattctc aagacttccc
     5221 aactgaagat gagcagtatg aagcatacaa ggctgttctt gaaggaatga acggtaaacc
     5281 tgttgtcgtt cgtacaatgg atatcggtgg agataaggaa cttccttact tcgatatgcc
     5341 tcacgaaatg aacccattcc ttggattccg tgctcttcgt atctctatct ctgagactgg
     5401 agatgctatg ttccgcacac aaatccgtgc tcttcttcgt gcgtctgttc acggtcaatt
     5461 gcgtatcatg ttcccaatgg ttgcgctctt gaaagaattc cgtgcagcga aagcagtctt
     5521 tgatgaagaa aaagcaaacc ttcttgctga aggtgttgca gttgcggata acattcaagt
     5581 tggtatcatg atcgagattc ctgcagcggc tatgcttgca gaccaatttg ctaaagaagt
     5641 tgacttcttc tcaattggta caaacgactt gatccaatat acaatggcag cagaccgtat
     5701 gaacgaacaa gtttcatacc tttaccaacc atacaaccca tcaatcctac gcttgattaa
     5761 caatgtgatc aaagcagctc acgctgaagg taaatgggct ggtatgtgtg gtgagatggc
     5821 tggtgaccaa caagctgttc cacttcttgt cggaatgggc ttggatgagt tctctatgtc
     5881 agcaacatct gtacttcgta cacgcagctt gatgaagaaa ctcgacacag ctaagatgga
     5941 agagtacgca aaccgtgccc ttacagaatg ctcaacaatg gaagaagttc ttgaacttca
     6001 aaaagaatac gttaattttg attaatcgaa aagtccctgc aactcagtta cagggacttt
     6061 ttttgatatt ttaaaaagaa ttttcaagaa aatctttctt ataaaaagtc caaccttgaa
     6121 aaagtagtgg tcagaacaaa aaatacttaa atggttcata aaattcttga caagttggag
     6181 atttaggagt aaactattaa ccagttaagt aatagagagg agtttctgca atttagaaat
     6241 gaattgcaac tagaaatatc aaatagaaag agagtttcga tgaaaattaa taagaaatac
     6301 cttgttggtt ctgcggcagc tttgatttta agtgtttgtt cttacgagtt gggactgtat
     6361 caagctagaa cggttaagga aaataatcgt gtttcctata tagatggaaa acaagcgacg
     6421 caaaaaacgg agaatttgac tcctgatgag gttagcaagc gtgaaggaat caatgctgag
     6481 caaatcgtca tcaagataac agaccaaggc tatgtcactt cacatggcga ccactatcat
     6541 tattacaatg gtaaggttcc ttatgatgcg attttcagtg aagaattact catgaaagat
     6601 ccaaactata agctaaaaga tgaggatatt gttaatgagg tcaagggtgg atatgttatc
     6661 aaggtagatg gaaaatacta tgtttacctt aaggatgctg cccacgcgga taacgtccgt
     6721 acaaaagagg aaatcaatcg acaaaaacaa gaacatagtc aacatcgtga aggtgggact
     6781 ccaagaaacg atggtgctgt tgccttggca cgttcgcaag gacgctatac tacagatgat
     6841 ggttatatct ttaatgcttc tgatatcata gaggatactg gtgatgctta tatcgttcct
     6901 catggagatc attaccatta cattcctaag aatgagttat cagctagcga gttggctgct
     6961 gcagaagcct tcctatctgg tcggggaaat ctgtcaaatt caagaaccta tcgccgacaa
     7021 aatagcgata acacttcaag aacaaactgg gtaccttctg taagcaatcc aggaactaca
     7081 aatactaaca caagcaacaa cagcaacact aacagtcaag caagtcaaag taatgacatt
     7141 gatagtctct tgaaacagct ctacaaactg cctttgagtc aacgacatgt agaatctgat
     7201 ggccttattt tcgacccagc gcaaatcaca agtcgaaccg ccagag
//
LOCUS       DAVDWT010000064         6877 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVDWT010000064 DAVDWT010000000
VERSION     DAVDWT010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.064"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..289
                     /locus_tag="VUW50_002028"
     CDS             <1..289
                     /locus_tag="VUW50_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983853.1"
                     /translation="ELNDIHKIEAVSKSTTGMLFHPGIFEMDAMRQAFFKRQAMHFFD
                     NEAEQQAYIEHAEKEYLEATITLKDILFNSKNGTQRVNKSCLIEKFEEAMQ"
     gene            286..486
                     /locus_tag="VUW50_002029"
     CDS             286..486
                     /locus_tag="VUW50_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983854.1"
                     /translation="MTLDLNNMTQAEFDEVMADIKARNPNIFQLISDFLDRKVTPEEM
                     DEFLKMERAEQVEYIKNYQARA"
     gene            488..703
                     /locus_tag="VUW50_002030"
     CDS             488..703
                     /locus_tag="VUW50_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983855.1"
                     /translation="MNELDISNTQAAVLIVILIGLLLYLNHRDRKKSAQIERENQQTI
                     KTPSEDLNPEYGRYIQLAGVNVWGGME"
     gene            700..996
                     /locus_tag="VUW50_002031"
     CDS             700..996
                     /locus_tag="VUW50_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983856.1"
                     /translation="MSYTMNLYPDIEKILDETYFTKEIFLATCMDNATKLAVTMGLLT
                     KQALDLETKTAILETLDTIIETQKAILQGVASGQITFVNKSKGEEELDEPIRER"
     gene            974..1315
                     /locus_tag="VUW50_002032"
     CDS             974..1315
                     /locus_tag="VUW50_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HET7983857.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGSEISLTTKLTERTVRDIIKR
                     LVVKHNIPIVGVRCGVFSGYFIPANKGELLDGAKAFYNQVQEESKRLAVLMNSDLESY
                     KETLKEVGGYV"
     gene            1308..1580
                     /locus_tag="VUW50_002033"
     CDS             1308..1580
                     /locus_tag="VUW50_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983858.1"
                     /translation="MFSLSRESEQDLAHGILEVVERYLEAREKVPPRLTKLINRVELK
                     EELKISDNTLNKWESQGLRRYQPPDDGSRMIYYLVTDIWKFLGVDE"
     gene            1577..2437
                     /locus_tag="VUW50_002034"
     CDS             1577..2437
                     /locus_tag="VUW50_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HET7983859.1"
                     /translation="MTIYEAKGFQSNLVYPFDKIEPFQYIERFKPLVVPESADPEEYK
                     RTQAPYCLSGKVMPEKNGSYKRNNSSLIYRDLIFLDYDDIQGTTEDFIEAVSSALFGY
                     SYILYPTIKHSIEKPRFRLVVKSNNVMNEATYKQVVKEIADKIGLPFDMASLTWSQLQ
                     GLPVTTGDPATYQKIVEHGLDYPVPKVEPRAKQGTTERYKPRVSGQRSMTMRIIDTLF
                     NGFGDEGGRNVALTRFVGLLFNKWVDCDLETAYELTKIANSVTVEPLPIEELDRSFSS
                     IARAEYRKRG"
     gene            2481..3959
                     /locus_tag="VUW50_002035"
     CDS             2481..3959
                     /locus_tag="VUW50_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET7983860.1"
                     /translation="MEVREQEHPPRTMKELENRIFKAGEEWRAEHTETKVNETTGDVT
                     EKVAIPQTFTVAKILSEIVTFTFISKSNIPDYSLLYIYDLDEGIYTASNDLFNLLCKT
                     FDVRIKPREWPQIKLMVRTLTKIRKPLESSNLIPVQNGIINLETKELFPFSPKYVITS
                     KISTAYHAPKRVPTDREGKTFDDWLNSIACNDSELVTLFWQIILEAINSNHTRNKFAI
                     FYGDGNNGKGTFQRFLINLIGESNISALKPAQFAEKHNLETLVGKVCNIGDEAPNEYL
                     KNPSDLMSITSGDTVLVNPKGRPAFEATFKLFNIFSGNYIPNGGNKTKGWYRRIMIVP
                     FNADFNGEKEKPWIKNEFLANDEVLEYALYKAINQEPFTHFIEPKAVKGLLEEYQEEN
                     DYLLSWVKHEYMERGWHELDVVPVFILTRTLKHYAEDMGIAKPNVYGAGKDTIRHLQQ
                     LTPNKYQLKKARVRIEDYDKLDPYGFERQKLGRVNHAVTKTK"
     gene            4324..4848
                     /locus_tag="VUW50_002036"
     CDS             4324..4848
                     /locus_tag="VUW50_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983861.1"
                     /translation="MTLKTFSDKAKTFNFTYEFKDLDTAMVAGHALLGYMTGTYCQPA
                     ISLTYKNKGTLVAEYVEDKKLNYIFKRICDSFKDYYKQPVNDEAFEERYKRERVLQLK
                     ESEDFESLLNKVTDYELELLDYAERLLSDKSIPMDSMTAFGTLEMLGNESINLLQKLD
                     VEGEYKGLADYSGQ"
     gene            4917..5420
                     /locus_tag="VUW50_002037"
     CDS             4917..5420
                     /locus_tag="VUW50_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983862.1"
                     /translation="MKRLAIETITKPMKLRGISKGIAELDGQGLEIDLDNLEIDFGGE
                     SFDLARIPGTKGGYRYFFLCPDCGRRCRLLYKRYLYFSCGTCLDIHKSTLNRSKTDCQ
                     YYWELALKEARKVEPGWSPRRGGYMFDGFPERPKYMKRDRYHKHYKKFLKYIEKGDRF
                     WLNGLRL"
     gene            6060..6479
                     /locus_tag="VUW50_002038"
     CDS             6060..6479
                     /locus_tag="VUW50_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HET7983863.1"
                     /translation="MTPEQVKEKLEGVKWINKEIKGLYLELEVLESGIIKKPTLSHSR
                     VQTSRENKTENNLISVLKLKEDTLQRIERLTEERMAISELIDKLANPLERSVLRLFYL
                     NDLVASEVAEEIDRSNTTVYIIRQKAIEHLTGIVNGD"
BASE COUNT         2362 a         1198 c         1541 g         1776 t
ORIGIN      
        1 tgaattgaat gacatccata aaatcgaagc ggtctctaaa agcactacgg gaatgctttt
       61 ccaccctggt atttttgaaa tggacgctat gcgacaagca ttctttaaac gccaagctat
      121 gcactttttt gacaacgaag cagagcaaca ggcgtatata gaacatgctg aaaaggagta
      181 tttagaggct actataacct taaaagatat tctttttaac tctaaaaatg gaactcagag
      241 agtaaataaa agttgtctta tagagaagtt tgaggaggca atgcaatgac actagaccta
      301 aacaacatga cacaagcaga atttgatgaa gtaatggctg acatcaaggc aagaaatcca
      361 aacatttttc aactgatttc tgatttttta gatagaaagg taacacccga agagatggat
      421 gagtttttga agatggagcg agctgaacaa gtggaatata ttaagaatta tcaagcgagg
      481 gcataacatg aatgaactag atataagtaa cacacaggct gctgtcttaa tcgtgatttt
      541 gattggctta ctgctttatt taaaccaccg agaccgcaaa aaaagcgccc aaattgagcg
      601 agaaaaccaa cagacgataa aaacacctag cgaggattta aaccctgaat atgggcgata
      661 tatccagctt gcaggggtca acgtatgggg agggatggaa tgagttacac aatgaattta
      721 taccccgata ttgaaaaaat actagatgaa acctatttta ctaaagagat ttttctagct
      781 acgtgtatgg acaatgcaac aaagttagct gtaactatgg gcttgttgac aaaacaagcg
      841 ttggatttag aaactaagac agctatcttg gaaacccttg atacaatcat agagactcaa
      901 aaagctattt tgcaaggggt agcgagcgga caaataacct ttgtaaataa aagtaaagga
      961 gaggaggaac tggatgagcc tatcagagaa cgataaacga gtattaagat taatcaaggt
     1021 aggggctgag aactccataa cagggtcaga aattagcctg acaaccaagc taacagaaag
     1081 aacagtacgt gacattatca agcgtctagt agttaagcac aacatcccta ttgtaggggt
     1141 tagatgcggc gtttttagtg ggtactttat tccggcgaat aaaggcgagt tactagatgg
     1201 cgctaaggct ttttacaacc aagtacagga ggagagcaag cgcctagcgg tgttgatgaa
     1261 tagcgaccta gaaagctaca aagaaacctt gaaggaggtg ggcggatatg tttagcctaa
     1321 gccgagaaag cgagcaagac ctagcgcatg gcattctgga ggtagtggaa agatacctgg
     1381 aagcgcgtga gaaagtaccg ccaagattga ccaagttaat aaatagagtt gaactcaaag
     1441 aggaattgaa aattagtgac aacacgctga ataagtggga aagtcagggt ttaagacgct
     1501 atcaaccgcc ggacgatggt tcaagaatga tttactattt agtaactgac atctggaagt
     1561 ttctgggggt ggatgaatga caatttatga ggctaaaggc tttcagagta atcttgtcta
     1621 tccatttgat aagattgagc cgttccagta cattgaacgc tttaagcctt tggtagtccc
     1681 tgaaagtgct gatccggagg aatacaaaag aacccaggcg ccatattgtt taagtggtaa
     1741 ggttatgccg gagaaaaacg ggagttacaa acgaaacaac tccagtctaa tttatcggga
     1801 tttgattttc cttgattatg acgatataca agggacgacc gaggacttca tagaagccgt
     1861 ttctagcgct ttatttggct actcttatat cttatacccg actatcaaac atagcataga
     1921 aaagccccgt tttcgccttg tggtgaaatc taacaacgtg atgaacgagg caacctataa
     1981 gcaggtagta aaagagattg ctgacaagat tgggctaccc tttgacatgg ccagtttaac
     2041 ctggtcacaa ctccaagggc taccagtcac aacaggcgac ccagcaactt atcaaaagat
     2101 tgtagagcat ggcctagatt atccagtccc caaggttgaa ccaagagcga agcaaggaac
     2161 tactgagcgt tataaaccta gagtaagtgg ccagaggtca atgactatga ggattattga
     2221 cacgcttttc aacggatttg gagacgaagg agggcgcaac gtagcgctga cacgctttgt
     2281 gggattactc ttcaataagt gggttgattg tgacctagag accgcctatg aactgacaaa
     2341 gatagcaaat agcgtgacag ttgaaccatt gcctattgaa gagctagacc gcagtttcag
     2401 tagtatagca cgggctgaat atagaaagag aggttagaac catagaacta gaagaattag
     2461 aaaacctaaa aagtgaaatc atggaggtca gagagcaaga acaccctcct agaacgatga
     2521 aggagcttga aaatcgtatc tttaaagctg gtgaagaatg gcgggcagaa cataccgaaa
     2581 ctaaggtaaa tgagaccaca ggggacgtta ctgaaaaggt tgccatacct cagacattca
     2641 ctgtagctaa aatactgagc gagattgtca cgtttacttt tatcagcaag agcaatatac
     2701 ctgattatag tttactctat atctacgatt tagacgaggg tatctataca gctagtaatg
     2761 atctatttaa tctattgtgt aagacttttg atgtgagaat taaacccaga gagtggccgc
     2821 agattaagtt aatggttaga acactaacaa agatacgaaa acctttagaa agttccaacc
     2881 tcattcccgt acagaacgga attatcaact tagaaactaa ggagctattc ccctttagtc
     2941 ctaagtatgt aattacaagc aagataagca cggcatacca cgcgcctaaa cgagtcccaa
     3001 cagatagaga gggaaaaact tttgatgatt ggctaaactc aatcgcttgt aatgatagtg
     3061 agctagtgac actgttttgg caaattatcc ttgaagctat caactctaac catacccgaa
     3121 ataagtttgc tatcttctac ggggacggta acaatggtaa agggacattt cagcgctttc
     3181 ttatcaatct gataggggaa agcaatatat cagccttaaa acctgcacag tttgctgaaa
     3241 agcataactt agaaaccctg gtaggtaaag tttgtaatat cggagatgaa gccccaaatg
     3301 aatacttgaa aaatccgtct gatttaatga gtatcactag tggcgataca gtactggtca
     3361 accccaaagg acgcccagcc tttgaggcta ctttcaagtt attcaatatc ttttcaggga
     3421 attatatccc caatggtggc aacaagacaa aaggctggta tcgtagaatt atgattgtcc
     3481 cttttaatgc tgactttaac ggggagaagg aaaagccttg gattaaaaat gaatttttgg
     3541 ccaatgatga agtattagag tacgcacttt acaaagctat caatcaagag ccttttacac
     3601 actttatcga acccaaagca gtcaaaggtc ttctagaaga ataccaggaa gaaaatgact
     3661 acttgctttc atgggttaaa catgaataca tggaaagagg ttggcatgag ctggacgtgg
     3721 tacccgtttt tatactgacc agaacactga agcattatgc tgaggacatg gggatagcca
     3781 agcctaatgt ttatggggca ggaaaagata ctatcagaca cctacaacag ttaacaccta
     3841 ataagtatca gcttaaaaag gcccgtgtta ggattgaaga ttatgacaaa ctggacccgt
     3901 atggttttga gagacaaaaa ctgggtcgag taaatcatgc tgtaactaaa acaaagtaat
     3961 gttgaccttc tcataatgct ctaaaccatt gatatgactg ggtttttacg gaatttgttg
     4021 accttcttgt tgaccttgtt ttaaagaagg tcaacatcct aaacccttga tagcactagt
     4081 tttatagcct ttatgttgac cttgttacct tctttttaaa aacaagaatg taaatgaaat
     4141 aaatatattt tatatataag ggcaaggact tcaaaagaag gtcaacatgg taacaaagtt
     4201 gtctaaaccc ttgggactgt aaggctaaac tacgttacct agaaggtcaa caaaagtaac
     4261 aagaaggtca acaaattaac aaatattaca taatttacct taaaataaag aaaggacaac
     4321 aacatgacat taaaaacatt ttcagataaa gcaaaaacat ttaatttcac ttacgaattt
     4381 aaagacctag atactgctat ggtggcaggt cacgcgctac tgggatatat gactggaaca
     4441 tattgccagc ctgcaatctc attgacctac aagaataaag ggacgctagt agctgagtac
     4501 gtggaggata agaaactaaa ctatatcttt aaacgtattt gtgacagttt caaagactat
     4561 tacaagcaac cagtgaacga tgaagcattt gaagaacgtt ataaacggga gcgcgtgctt
     4621 caactcaaag agtcagagga ctttgagagc ttgctgaata aggtcacaga ctacgagctg
     4681 gaactattag actatgctga acgcttgctt tctgataagt ctatccctat ggactctatg
     4741 actgcttttg gtacgttgga aatgctgggt aatgaaagta ttaacctact ccaaaaattg
     4801 gacgtagagg gcgaatataa aggccttgct gattattctg gtcaataaac acaggggagc
     4861 aatccccttt tgacatttag gcaatagttt tgggtttttc catatagggg gaaataatga
     4921 agcggttagc aatagaaaca atcactaaac caatgaagct gagaggaata tctaagggta
     4981 tagccgagtt ggacggtcaa ggtttagaga ttgatttgga taatttagag attgattttg
     5041 gtggagagtc atttgattta gcaagaatac caggaactaa gggtggttat cgttatttct
     5101 ttctttgccc tgattgtggt agacggtgca ggttgcttta taaacggtat ttgtatttta
     5161 gttgcggtac ctgtctagat atccacaaga gcaccctgaa ccgcagtaaa acggattgcc
     5221 aatattactg ggagcttgca ctgaaagaag ctagaaaggt agaaccaggg tggagtccta
     5281 gacgtggtgg atatatgttt gatggtttcc ctgaaagacc aaagtatatg aagcgtgaca
     5341 ggtatcataa gcactataag aagtttttga agtatattga aaaaggggat agattttggc
     5401 tgaatggttt gagattgtag gacgatatga agtagtgtgc aatttacaaa acaaacaaat
     5461 actacatatt agacggtttg aatgttaaaa aagctagata tatcaaggat ttaagtaagt
     5521 agagcgttcc aaaaaagggt gcggtaaggg tgaggtagca tatataaaaa gtacaaggtt
     5581 accacaaggt agagaggttt accgagagtt agaaaaggtg agattttgct taggtagtgg
     5641 cagtaaatat gcctggaaaa tgtcagggtt atgtgtgttg aactccgagc gaacgccgag
     5701 aaagtgaata tatcccccta tcatttatca agcagataag agcaataata accctcctta
     5761 actccctgag aaacgacaag cagataagcg aagaaataac ttgccttaat tgcttgcaga
     5821 atgacaagga aataagggag ataaaaacat cccttaattg cttatcaaac cacaaggaga
     5881 tacgggggct atctccctcc ccacgcactt ctatgagctt cacaccgtca ttgtacattt
     5941 tttctcacgg ggaagcattt gaagctagag cgaacataga gaaaacgaaa aaagccaagg
     6001 cactccgcct cagctaatag ttctcacaaa gactattata tcataaagga ggccgaggca
     6061 tgacaccaga gcaggtaaaa gaaaaactag agggcgttaa gtggataaac aaagagatca
     6121 aaggattata tttggaattg gaagtcctgg agagtggtat tatcaaaaag ccaacactaa
     6181 gccatagcag ggtgcagaca agcagagaga acaagacaga gaacaatctt ataagtgttc
     6241 tgaagctaaa agaggataca ctccagagaa ttgagcgcct taccgaggaa agaatggcaa
     6301 tatctgagct aatcgataaa ctggccaatc cgcttgagcg ttctgttcta agactttttt
     6361 acttgaatga tcttgtagct tcggaggtgg ctgaagaaat agatagatca aatactacag
     6421 tatacattat aaggcaaaaa gctatagaac acttaacagg tattgtaaac ggggattgat
     6481 ttaaccttac cacggaatta ggagagtatg gggaactcta agccccatat ttgaccctat
     6541 ttcgcttggt ttcgtaccgg acgattaaaa caccatggat aaactagaag cccttagaaa
     6601 cgaatcaggg gggcttttgt gaggtcataa tcttataaac cacgcgcttc atggtataat
     6661 atacctatca gcaaccagca ataaaaaaag cacgtttgac cgtgctagtt tcttgcctgc
     6721 tgaactcgtc atttattgcc cttttgtggg gctttttttg tggacttttt gagaattttt
     6781 aagaactttt aatgatttct aatcaaatga gatttttcaa aaagtcagta atatcaaggg
     6841 tttaggcgct ataaacaagt ttaaaagcgt gtagatt
//
LOCUS       DAVDWT010000065         6578 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVDWT010000065 DAVDWT010000000
VERSION     DAVDWT010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.065"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            4..552
                     /locus_tag="VUW50_002039"
     CDS             4..552
                     /locus_tag="VUW50_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET7983864.1"
                     /translation="MGSVHLLGVLFPDSTFLNAFESAIVAPLVEESLKLLPLVFVLAL
                     IPVRKLKSLFLLGIASGLGFQMIEDIGYIHTDLPEGFDFTISRILERIISGIASHWTF
                     SGLAVVGVYLLYRAYKGQKVGKKQGLIFLGLALGTHFLFNSPFVELETELPLAIPVVT
                     AIALYGFYHAYCFVEKHNELMT"
     gene            697..1665
                     /locus_tag="VUW50_002040"
     CDS             697..1665
                     /locus_tag="VUW50_002040"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET7983865.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            1783..2136
                     /locus_tag="VUW50_002041"
     CDS             1783..2136
                     /locus_tag="VUW50_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET7983866.1"
                     /translation="MTVRHPGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAQNVTIL
                     EGSNIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQV
                     NQNLEKIRQREGDTH"
     gene            2351..2467
                     /locus_tag="VUW50_002042"
     CDS             2351..2467
                     /locus_tag="VUW50_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET7983867.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            3319..5952
                     /gene="polA"
                     /locus_tag="VUW50_002043"
     CDS             3319..5952
                     /gene="polA"
                     /locus_tag="VUW50_002043"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET7983868.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNMSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDQLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLSQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYLQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            6037..6474
                     /locus_tag="VUW50_002044"
     CDS             6037..6474
                     /locus_tag="VUW50_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET7983869.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
BASE COUNT         1839 a         1140 c         1572 g         2027 t
ORIGIN      
        1 tttttggggt cagtacattt actgggggtt cttttcccag atagtacatt tttaaatgcc
       61 tttgaaagtg ctattgtggc tcctttggta gaagaatcct tgaaattatt gccacttgtt
      121 tttgttttgg ctttgattcc tgtgcgaaaa ttaaaatctt tgtttttact tggaattgct
      181 tccggtttgg gattccaaat gattgaggat attggttata ttcatacgga tttgccagag
      241 ggctttgact ttactatttc gcgaatttta gagcgtatca tctcaggaat tgcctctcac
      301 tggacttttt caggtctagc tgtagtaggt gtttacttgc tttacagagc ctataaagga
      361 cagaaggttg gcaagaaaca gggccttatt tttctaggtt tagccttggg aactcacttc
      421 ttgtttaact ctccttttgt ggagttggaa acagagttgc ctttagcgat tccagtggtt
      481 acggctattg ctctctatgg tttttatcat gcttattgct ttgttgagaa acacaatgag
      541 ttgatgacct agaatatttt tcaaaagaat gatgcaaggg tacaaatatg gtgcccttct
      601 tttatttttg attgaaaaat agtgcaaaaa gcgctacaat ggtagatgga aaatcttgtg
      661 aaaagcacaa gttatacata tataccggag gaaatcatgt ctttttctga tttaaagcta
      721 tttgcccttt cttctaataa agaattggca gaacgtgtgg cgcaggagat tgggatagag
      781 ttggggaaat caagtgttcg ccaattttca gatggagaga ttcaggtcaa cattgaagaa
      841 tcaatccgtg ggaaacacgt ctttatccta caatcaacta gttcgcctgt aaatgacaat
      901 ctgcttgaaa ttttgattat ggtagatgct ttgaagcgtg cgagtgcaga atctgtcaat
      961 gttgtcatgc cttactatgg gtatgcacgt caggatagaa aggcgagagc gcgtgagcca
     1021 atcacttcaa aacttgtcgc aaatatgctt gaagtagctg gagtggatcg tttattgacc
     1081 atcgacttgc atgctgcgca aattcaagga ttctttgata ttcctgtgga tcatttgatg
     1141 ggtgctcctc tgattgcaga ttattttgag cgtcgtggta tggttggttc tgactatgtg
     1201 gttgtcagcc cggaccatgg aggggtgact cgtgctcgta agttggcaga atttttgaaa
     1261 acttctatcg ctattattga taaacgtcgt agcgttgata agatgaatac tagtgaagtt
     1321 atgaacatca tcggtaaggt tgaaggcaag acttgtatct tgattgatga tatgattgat
     1381 accgctggaa cgatttgtca tgcggcagat gctcttgcgg aagctggtgc tgttgaagtc
     1441 tatgcaagct gtacgcaccc agttctttct ggtcctgcta cggacaatat ccaaaaatca
     1501 gctattaaga aattggttgt tttggatacc atctatctgc cagaagagcg tttgattgat
     1561 aagattgagc agatttcaat cgctcatctc ctaggggatg ctatcgtacg tattcatgaa
     1621 aaacgaccac tttctccact tttcgatatt gagaaaaaaa tttaatgacc aagcctgaga
     1681 tgattctcag gttttttcgt cttttttccg aataaataga tagtagcggt gaatctagta
     1741 aagctagatt taaaactgtg atataataaa aagagaagaa atatgactgt acgtcatccg
     1801 ggcattagcc caaccaatga cttggtagct aagaaaatct ttagcaatcc agaaattact
     1861 tgtcaattta ttcgcgatat gctggatttg ccagcccaaa atgtgaccat tttggagggt
     1921 agcaatattc acgtattact ctccatgcct tactcggtgc aggattttta taccagtata
     1981 gacgtcttgg cggagttgga taacggtact caagtaatta ttgagattca agtccatcat
     2041 cagaattttt tcatcaatca cttgtgggct tacctgtgca gtcaggttaa tcaaaatctt
     2101 gaaaaaattc gtcagcgaga aggtgatact cactagatct acaaacacat cgctcctgtt
     2161 tacgtcattg ctatcgtgga tagcaattat ttctcagatg gcctggcttt tcatagcgtt
     2221 agtatgcgcg aagacacaac aagtgaggta ttggcgatta ccaacaatgg acaggaaaac
     2281 catctggtta agatggcatt cttggaatta aaaaatacag agaaaccagc aaagacaagg
     2341 ttcgcaagcc atggttggag tttttcggca acaagccctt tacccagcaa ccgcaacgag
     2401 ccattaccca agcaaatcaa ctgctggact acaagagctg gtccgaggag gacaggaaaa
     2461 tgtttagtca actacatatg cgagaagaac aagtcttgtt agcacaggac tatgcgaagg
     2521 tattgaacaa ggactagagc gtggtattga acgtggtcgt gcagagggtt gtgaacaagg
     2581 acgagaagaa ggccttgaag agggattaaa agtaggttta gtaaatctag tacgtcaagg
     2641 tcttctaaca cctgaggttg cgagtgagca attaggcatg accgtggctg agtttgagtc
     2701 cttgctatga gttcatactt tatatagtag aaagttgatg ggatggcaag tttattatca
     2761 aaatacttgt catttttgct tgacatttct atctttttat aagatatagt aaaatgaaat
     2821 aataacagga caaatcgttc aggacagtca aatcgatttc taacaatgtt ttagaaatcg
     2881 aggtgtacta ttctagtttc aatctactat agtattagcg aagtaagtta tgtctcgcta
     2941 ttcgtacagt ttggatagtc gcaagattgt ctttgaaatt tcctgtttta aagagaaaaa
     3001 agcctctttg acgctatttt ttcatctttt tgaaagttct ataatgaagt tagccaccca
     3061 gccctccttt tcctccttct atagtgagtt gaaataagat gtgaaacaac tctatcagga
     3121 aagtcaaatt aatttataga aatattttag cagtcaaggt gtactattat agattcaata
     3181 tactatatta tagcacattt ttaaatctag gtttactaga ttctctggct ctatctattt
     3241 gttcggaaaa agtttgaaaa atacttgctc tagctcttcc caatggtatt ttttgattct
     3301 ttcctttata atgggtgtat ggataagaaa aaattattat tgattgatgg gtcttctgta
     3361 gcttttcggg cgttttttgc gctgtatcag cagttggacc gttttaagaa tgcggctggt
     3421 ttgcatacca atgcgattta tggttttcag ttgatgttga gtcatttgtt ggagcgggtt
     3481 gagccgagtc atattttggt ggcttttgat gcgggaaaga cgaccttccg gacagagatg
     3541 tatgcggact ataagggtgg tcgggccaag actcctgatg agtttcgtga gcaatttcct
     3601 ttcattcgtg agttgctgga tcatatgggg attcgtcact atgagctcgc tcagtatgag
     3661 gcggatgaca tcattgggac gctggataag ctagcagagc aggatggttt tgatattacc
     3721 attgtcagtg gggacaagga tttgattcag ctgacggatg agcatacggt ggttgaaatt
     3781 tccaagaaag gtgtggctga gtttgaggcc tttacgccag attacctcat ggaagaaatg
     3841 ggcctcacac cagctcagtt tatcgatctc aaggcgctca tgggtgataa gtcggataat
     3901 atccctgggg tgaccaaagt cggtgaaaag acgggtatta agctcttgct ggagcatggt
     3961 tcgcttgagg ggatttatga aaatattgat ggaatgaaga cttctaagat gaaggaaaat
     4021 ctcatcaatg acaaggaaca ggcctttttg tctaaaacac tagcgaccat tgataccaag
     4081 gcaccgattg cgattggttt agaggacttg gtctatagtg gtccagatgt tgaaaatctt
     4141 gggaaatttt acgatgagat gggattcaaa cagctcaagc aggctttaaa tatgtcgtca
     4201 gctgatgtgg ctgagggttt ggattttact attgttgacc aaatcagtca agatatgctg
     4261 agtgaagagt ctatcttcca ctttgagctt tttggtgaga attaccatac agataatttg
     4321 gttggatttg cctggtcttg tggggatcag ctctatgcca cagacaagct tgagctgttg
     4381 caagacccga ttttcaagga tttcttagaa aaaacatctc tgagagttta tgactttaag
     4441 aaggttaaag ttcttttgca acgttttggt gtagatttgc aggcgcctgc ttttgacatc
     4501 cgtttggcta aatacctcct ttcgactgtg gaggacaatg aaattgcgac catcgctagt
     4561 ctttatggtc agacttactt ggttgatgat gaaactttct acggtaaggg tgttaaaaag
     4621 gccattcctg aacgtgagaa attcttggaa cacttagctt gtaaacttgc tgttttggta
     4681 gaaacagagc ctattttact tgaaaaactc agcgaaaatg ggcaattaga gcttctttat
     4741 gatatggagc aacctctggc ttttgtcctt gccaagatgg aaattgctgg aattatggtt
     4801 aagaaagaga ccttgcttga gatgcaggct gaaaatgagc ttgtcattga aaaactgtct
     4861 caagagattt acgagctggc tggtgaggag tttaatgtca actcgcctaa gcagttgggc
     4921 gtgcttctct ttgagaaatt gggacttcct ctagaataca ctaagaaaac caagacaggt
     4981 tattcgacag cagtggatgt tttggagcgt ctcgctccta ttgctccgat tgttaagaaa
     5041 atcctggatt accgtcaaat tgctaagatt caatctactt atgtaattgg cttgcaggac
     5101 tggattttgg ctgatggaaa gattcatact cgctatttgc aggatttgac ccagaccggg
     5161 cgtttgtcta gtgtggatcc aaacttgcaa aatattcctg cccgattgga acaggggcgc
     5221 ttgattcgga aggcttttgt gccagagtgg gaggatagtg tgctactcag ctctgactat
     5281 tcacagattg aattgcgcgt tttggcgcat atttctaagg atgagcactt gattaaggcc
     5341 ttccaagagg gggcagatat ccatacttcg acagccatgc gggtctttgg cattgagcgt
     5401 cctgataatg tgactgcaaa tgaccgtcgt aatgccaagg cagttaactt tggagtggtt
     5461 tatgggattt cagactttgg cttgtctaat aatttgggaa ttagtcgtaa ggaagccaaa
     5521 gcctacattg atacctactt tgaacgtttt ccaggtatta aaaactacat ggatgaagtg
     5581 gtgcgggagg cgcgtgataa gggctatgta gagaccctct ttaagcgtcg ccgtgagttg
     5641 ccagatatca attcgcgcaa cttcaatatt cgtggttttg cggagcgaac tgctatcaac
     5701 tcacctatcc agggttcggc agcagatatt ctcaagattg ccatgattca gctggataaa
     5761 gccttggttg caggtggtta tcagactaag atgctgttac aagtgcacga tgaaatcgtc
     5821 cttgaagtgc ctaaatctga attggtagag atgaaaaaat tggtgaaaca aaccatggaa
     5881 gaagccattc aactcagtgt tcctcttatc gcagatgaga atgaaggagc aacctggtac
     5941 gaggctaaat aaaaaggggg ctagtcctcc ttttttgtag tagaattctg caaacctttt
     6001 caaaatgtgc tatactgatg aaaaaggagg atttctatga gtcaagaatt tatcaatcca
     6061 agtgatggcg tgattcgtca gtatctcgca acgagtaaaa cccttgctgt ggtgggtttg
     6121 tctgaccgtg aagaaacaac cagcaatcga gtgacaaagg aaatgcaggc tcggggctat
     6181 aaaatcatcc cagtcaatcc caaggcggca ggtggcgaaa tcttgggtga aaaggcttat
     6241 gctagcctcg ctgaaattcc ttttcctgta gatattgtca atgtttatcg tcgcagtgaa
     6301 tttctgcccg atgtggcgcg tgattttctc aaagctgatg ccaagatttt ttgggcacag
     6361 ctaggacttg aaagtttaga agcgaaagag attttgcgtg atggtggatg tgatgatatc
     6421 gtgatgaatc gctgtatcaa gagagaacat actcgcttga ttgaggaagc ataagaaaaa
     6481 ggtagctgat gggctacctt ttgtgttata ctcaatgaaa atcaaagagc aaactaggaa
     6541 actagccgcg ggctgctcaa aacactgttt tgaggttg
//
LOCUS       DAVDWT010000066         6450 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVDWT010000066 DAVDWT010000000
VERSION     DAVDWT010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.066"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            1..363
                     /locus_tag="VUW50_002045"
                     /pseudo
     CDS             1..363
                     /locus_tag="VUW50_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            530..730
                     /locus_tag="VUW50_002046"
     CDS             530..730
                     /locus_tag="VUW50_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983870.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            772..1176
                     /locus_tag="VUW50_002047"
     CDS             772..1176
                     /locus_tag="VUW50_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983871.1"
                     /translation="MDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETNSVPA
                     GVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKAQNLL
                     QETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1407..2114
                     /gene="ccdA2"
                     /locus_tag="VUW50_002048"
     CDS             1407..2114
                     /gene="ccdA2"
                     /locus_tag="VUW50_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET7983872.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            2125..2682
                     /gene="sdbB"
                     /locus_tag="VUW50_002049"
     CDS             2125..2682
                     /gene="sdbB"
                     /locus_tag="VUW50_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HET7983873.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            3056..4447
                     /locus_tag="VUW50_002050"
     CDS             3056..4447
                     /locus_tag="VUW50_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET7983874.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYRIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4590..5507
                     /gene="adcAII"
                     /locus_tag="VUW50_002051"
     CDS             4590..5507
                     /gene="adcAII"
                     /locus_tag="VUW50_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HET7983875.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5515..>6450
                     /gene="phtD"
                     /locus_tag="VUW50_002052"
     CDS             5515..>6450
                     /gene="phtD"
                     /locus_tag="VUW50_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HET7983876.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQEH
                     SHNHGGGSNDQAVVAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNE
                     LSASELAAAEAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISS
                     LLRELYAKPLSERHVESDGLIFDPAQITSRTARGVA"
BASE COUNT         1979 a         1172 c         1370 g         1929 t
ORIGIN      
        1 cgctcaaagc tatgggctac actcgaaaaa aagaaccaca cctactatga acaagaccca
       61 gaaaaagtag ccttatttct taagaatttt aatagtttaa agcacctagc acctgtttag
      121 attgacgaaa caggattcga tacttatttt tatcgagaat atggtcgctc attaaaaggt
      181 cagttaataa gaggcaaagt atctggaaga agatatcaga ggatttcttt ggttgcaggt
      241 ctaacaaatg gtgaattaat cgctccaatg acttacgaag agacgatgac gagcgacttt
      301 tttgaagctt ggtttcagaa gtttctctta ccaacattaa ccacaccatc ggttattata
      361 gtaaaatgaa ataagaatag gacaaatcga tcaggacagt aaaatcgaat tctaacaatg
      421 ttttagaagt ccatatgtac tattctagtt tcaatatacc ataactgttc ctagtcaacc
      481 tgcaagccct gtcccagctc caagctcagc tccgactcaa gatcctagag tggtagcaag
      541 cgcttcggct agctcaacga gcacacaagc tcaagagcaa gttgacaagt ctgaacttcg
      601 tgccttgagt caagagttag accaacgctt gaaagctttg gcaacagtat ctgatccgaa
      661 aatcgatgca accaaggctg tcctcctaga tgctcaaaaa gctccggaag atagtgcctt
      721 gacagagtaa ggtctgcata ctgcagtaga gtctgtgaag gctgggctgc cttggactct
      781 ctcaaagatg tgaaagcaaa tgctagcgac agcaagcctg cacaggacaa gaaggatgca
      841 aaacaaggaa cggaagatag taaggattca gataagatga ctgaaacaaa ctcagttccg
      901 gcaggagtga ttgtggtcag tctacttgcc ctcctaggcg tgattgcctt ctggctgatt
      961 cgccgtaaga aagagtcaga aatccagcaa ttaagcacgg aattgatcaa ggttctagga
     1021 cagctagatg cagaaaaagc ggataaaaaa gtccttgcca aagcccaaaa ccttctccaa
     1081 gaaacccttg atttcgtgaa agaagaaaat ggctcagcag agacagaaac taaactagta
     1141 gaggagctta aagcaatcct tgacaaactc aagtaaaaca tgatttatga aatgtcagac
     1201 ctaaaagaca ttttcgttca caaaagcagc gatgagtaat ccttgtcgtt gctttttggc
     1261 tattttgggg gataagatcc gccttgacta gcaaaagaaa agattggtcc aatagtgccc
     1321 gatacgattt tgcgtagcag aaagcttaca aataaaattg aatatagtaa aataggatta
     1381 gaattattaa gaaagttggt tctttattgg aaacgatagt atttttaatc tctgtttttc
     1441 tagcaggtgt tttatccttt ttttctcctt gtatttttcc tcttctgcca gtctatgctg
     1501 ggattttatt ggatgatcaa gaaagtgcaa aaagcttttc tttgtttggg agaaaggttc
     1561 tctggtcagg cttgattcga acactttgct ttatcgctgg tatctctctc attttcttta
     1621 ttctaggctt tggtgctggt tactttggtc atattctcta tgcaaattgg tttcgatatg
     1681 gcatgggagc tattattatc attttgggtc ttcaccagat ggaaattttt catttgaaga
     1741 aattagaagt tcaaaaaagt tttaccttta aaaaatcaga ttctaatcgt tattggtcag
     1801 cttttttact tggtattacc tttagctttg gttggacacc ttgtattggt ccagttttaa
     1861 gttctgtttt agcacttgcg gcttctggag gcaatggcgc ttggcaagga gcgatttata
     1921 ctctcattta cactctgggc atggcccttc ctttcttggt attggcacta gcttcaggtc
     1981 tagtcatgcc atattttagt aaaatcaagc gtcatatgat gctactaaag aaaattggtg
     2041 gtttcctcat tgttttaatg ggaattttgt tactattagg acaagtaaat gttctagctg
     2101 gaatttttga ataaaggagg acaaatgaaa aaagtaatgt ttgctggctt aagtctcttg
     2161 tcattagttg tattgatggc ctgtggtgag gaagaaacta aaaagactca agcagcacaa
     2221 cagccaaaac aacaaacgac tgtacaacaa attgctgttg gaaaagatgc tccagacttc
     2281 acattgcaat ccatggatgg caaagaagtt aagttatctg attttaaggg taaaaaggtt
     2341 tacttgaagt tttgggcttc atggtgtggt ccatgcaaga aaagtatgcc agagttgatg
     2401 gaactagcgg cgaaaccaga tcgtgatttc gaaattctta ctgtcattgc accaggaatt
     2461 caaggtgaaa aaactgttga gcaattccca caatggttcc aggaacaagg atataaggat
     2521 atcccagttc tttatgatac caaagcaacc accttccaag cttatcaaat tcgaagcatt
     2581 cctacagaat atttaattga tagccaagga aagattggaa agattcaatt tggtgctatc
     2641 agtaatgcgg atgcagaagc agcatttaaa gaaatgaact agaatcgata aaatctgatt
     2701 acaagatgta gtcagatttt ggtaacttct aaaactaact tccagtttcc cacaacctag
     2761 cttttagtat gagaaaaacg cgtgacatca gtggaaatcc gtcttagact aacttccact
     2821 ggttttcttt ttcttgatat ataagggttc aaaagcgaaa agactcagaa aaagtgcacg
     2881 acaaatagcc ctaaaacaga gtcgtcttag agtaacttcc agttttggga gagagatgga
     2941 agttactttg agaagttacg gtagtcagat ttttttagaa aaacatttta taaatattca
     3001 catatctcct atatttatgg taaaatagaa ttatcagttt attttggagt caaagatgaa
     3061 tatatttaga acaaagaatg ttagtttaga taaaacagag atgcataggc atttgaagtt
     3121 atgggatttg attttgctgg gtatcggagc catggtaggg acaggcgtct ttacaatcac
     3181 aggtactgca gctgcaacac ttgctggccc agccctagtg atttcaatcg ttatttctgc
     3241 cttgtgtgtg ggattatcag ccctcttttt tgcagaattt gcctcgcgag tacccgctac
     3301 aggaggtgcc tatagttacc tctatgctat cttaggagaa ttccctgcct ggttggctgg
     3361 ttggttaacc atgatggagt tcatgacagc catatcaggc gtagcttcgg gttgggcagc
     3421 ttattttaaa ggcttgctct ctcaatacag gatagccttt cctcaggctt taaatggtac
     3481 ctttaatcct caagcaggga catttgttga tttattgcct attcttgtct tggtcttggt
     3541 gacttcgctt gttttactaa atgccaaggc agccttacgc tttaattcga ttctagttat
     3601 tttgaaattt tccgctttag ctctctttgt tttagtagga atttggaata ttaagtttga
     3661 taattggagc aattttgctc cttacggttt tggacaaatc tatggagcta gcactggtat
     3721 tatggctggt gcgtccttga tgttcttcgg ttttttggga tttgaatcca tctctatggc
     3781 tgtagatgaa gtcaagactc ctcaaaaaaa tattcctaga gggattgtct tatcgctttc
     3841 tatcgtaacc attctctatg ccttggtgac ccttgttttg actggtgtgg ttcactatag
     3901 tcatttaaat gtcgatgatg ccgttgcctt tgcccttcgt agtgttggga ttagttgggc
     3961 agccaactat gtgtcattag tggctatctt gaccttgatt acagtttgta tttcgatgac
     4021 ctatgcccta tcgcgtatga tttacagttt agcacgtgac ggcttagtgc ctgctgcctt
     4081 taaggaactg acgaagacta gcaagatacc aaagaatgct actatcttga caggtctagc
     4141 ttcagcagta gcagcaggaa tgttcccact agccagtatt gcagcctttt taaatatttg
     4201 taccttagcc tacttgatca tgttggctta tggtctgatt cgcttacgga aagaaaaagg
     4261 aatgcccaaa gctggggaat ttaaaacacc attggtacct ctgttaccaa ttttatcgat
     4321 catcatttgt ttatccttca tgttacagta taatatgaat acctggattg ctttcctagt
     4381 tgcgttgtta gtaggaagta ttatttactt cacttatggc tataagcatt ctaccataga
     4441 agaataaagt gaaagtctat tgagttgttg acactcagca gatttttata tgtgaaagaa
     4501 atttcaaatt ttttctaaaa atatcttgac agattaaatt ttcaggagta gaatatttac
     4561 tagttaatta aatggttaag gagttgttca tgaagaaaca aaatttattt ttagtcctgt
     4621 taagtgtctt tcttttgtgc ttgggggctt gtggtcaaaa ggaaagtcag acaggaaagg
     4681 ggatgaaaat tgtgaccagt ttttatccta tctacgctat ggttaaggaa gtatctggtg
     4741 acttgaatga tgttcggatg attcagtcaa gtagtggtat tcactccttt gaaccttcgg
     4801 caaatgatat cgcagccatc tatgatgcag atgtctttgt ttaccattct catacactcg
     4861 aatcttgggc aggaagtctg gatccaaatc taaaaaaatc caaagtgaag gtcttagagg
     4921 cttctgaggg aatgaccttg gaacgtgtcc ctggactaga ggatgtggaa gcaggggatg
     4981 gagttgatga aaaaacgctc tatgaccctc acacatggct agatcctgaa aaagctggag
     5041 aagaagccca aattatcgct gataaacttt cagaggtgga tagtgagcat aaagagactt
     5101 atcaaaaaaa tgcgcaagcc tttatcaaaa aagctcagga attgactaag aaattccaac
     5161 caaaatttga aaaagcgact cagaaaacat ttgtaacaca acatacagcc ttttcttatc
     5221 tagcgaagag atttgggctt aatcaacttg gtattgcagg tatctctcct gaacaagaac
     5281 caagtccacg acaactaaca gaaattcagg aatttgttaa gacctataag gttaaaacga
     5341 tttttacaga aagtaacgct tcttcaaaag tagctgaaac tcttgtcaaa tcaacaggtg
     5401 tgggtcttaa aactctgaat cctttagagt cagacccaca aaatgacaag acctatttag
     5461 aaaatcttga agaaaatatg agtattctag cagaagaatt aaagtgagga aagaatgaaa
     5521 atcaataaaa aatatctagc aggttcagtg gcagtccttg ccctaagtgt ttgttcctat
     5581 gaacttggtc gtcaccaagc tggtcaggtt aagaaagagt ctaatcgagt ttcttatata
     5641 gatggtgatc aggctggtca aaaggcagaa aacttgacac cagatgaagt cagtaagagg
     5701 gaggggatca acgccgaaca aatcgtcatc aagattacgg atcaaggtta tgtgacctct
     5761 catggagacc attatcatta ctataatggc aaggtccctt atgatgccat catcagtgaa
     5821 gagctcctca tgaaagatcc gaattatcag ttgaaggatt cagacattgt caatgaaatc
     5881 aagggtggtt atgttatcaa ggtagatgga aaatactatg tttaccttaa ggatgcagct
     5941 catgcggata atattcggac aaaagaagag attaaacgtc agaagcagga acacagtcat
     6001 aatcacgggg gtggttctaa cgatcaagca gtagttgcag ccagagccca aggacgctat
     6061 acaacggatg atggttatat cttcaatgca tctgatatca ttgaggacac gggtgatgct
     6121 tatatcgttc ctcacggcga ccattaccat tacattccta agaatgagtt atcagctagc
     6181 gagttagctg ctgcagaagc ctattggaat gggaagcagg gatctcgtcc ttcttcaagt
     6241 tctagtcata atgcaaatcc agctcaacca agattgtcag agaaccacaa tctgactgtc
     6301 actccaactt atcatcaaaa tcaaggggaa aacatttcaa gccttttacg tgaattgtat
     6361 gctaaaccct tatcagaacg ccatgtggaa tctgatggcc ttattttcga cccagcgcaa
     6421 atcacaagtc gaaccgccag aggtgtagct
//
LOCUS       DAVDWT010000067         5127 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVDWT010000067 DAVDWT010000000
VERSION     DAVDWT010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.067"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..497
                     /locus_tag="VUW50_002053"
     CDS             <1..497
                     /locus_tag="VUW50_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET7983877.1"
                     /translation="YPNPQKRLEAGFDKLIEIKRLTASKIQDILSVAPRSIGTTSPAR
                     EFEIIENIKHYKRLIDKAEKCVNDLMAEFNSVITTVTGIGNRLGAVILAEIQNIHAFD
                     NPAQLQAFAGLDSSIYQSGQIDLAGRMVKRGSPHLRWALIQAAKACARFSPAFKAYLK
                     TKLE"
     gene            complement(798..2687)
                     /locus_tag="VUW50_002054"
     CDS             complement(798..2687)
                     /locus_tag="VUW50_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET7983878.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKIQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKEQSKQLLTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFERDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2825..4390
                     /locus_tag="VUW50_002055"
     CDS             2825..4390
                     /locus_tag="VUW50_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET7983879.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKDDTRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTITGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWILNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            4390..>5127
                     /locus_tag="VUW50_002056"
     CDS             4390..>5127
                     /locus_tag="VUW50_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET7983880.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEEIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLI"
BASE COUNT         1580 a         1173 c          956 g         1418 t
ORIGIN      
        1 gctaccctaa tcctcagaaa aggcttgagg caggatttga taagctgata gaaattaagc
       61 gattgaccgc ttctaaaatt caggatatcc tctcagtcgc acctcgttct atcggaacaa
      121 catctcctgc tcgtgaattc gaaatcatcg aaaacatcaa acattacaag aggctcattg
      181 acaaggcgga aaaatgtgtc aatgacttga tggctgagtt caactcggtc atcacgacgg
      241 ttactgggat tgggaatcgt ttaggggcgg tcattttagc cgagattcaa aatattcatg
      301 cctttgataa tcctgctcaa ttacaagctt tcgctggact ggattcttct atttatcagt
      361 caggtcagat tgatttagct ggaagaatgg tcaaacgggg ttctcctcat ctgcggtggg
      421 cactcataca agctgccaaa gcatgcgctc gcttttcacc tgcttttaag gcctatctta
      481 agactaagtt agaataagaa aaacattaca atgtagccat catccacctt gcaaaaaaac
      541 ttatccgaac cctgttttat atcctaaaaa agagctgcca tttgacgaac aaaaagtgat
      601 gtaatttctg aaatcaacac aggattacct ttgaaaaaat ggactttttc aagggctttt
      661 tgccgtgctc taaaatcatc gccaaatcat caaattgaaa taagaaatag tttagataaa
      721 attattgact tttcttatag tttgtctttc ttctatcttc cattatatca tactttttca
      781 gtctatggta gctctcttta aaaggctccg atactgccgc cacctccgcc tccagagaag
      841 ccaccgccag aacttccact tccagaagat acggagtagg tacttgctgt atttgcgaca
      901 ctagcataat ggctcatttg cgcgcttgaa tgataaaaca tactgtgcca gccataagct
      961 acatagagat tgatatctgg attttcaact tgaatctgat gaaccttcat caaatgacta
     1021 accttgtccg catagccaaa taaggttgca tagaccaaga gacgattcca aacaacaata
     1081 ctttccaatt cagccttatc cagtcgtgca atctcacgta acatattttc aaaactggtc
     1141 cagagatagt agacctcagc tcccgcttca tttagaacac catcacgatt atctagtcga
     1201 agcttccaat aatagaaaac agacaaaact aaccctagaa aaccaagtat tggcaaaggg
     1261 aggtaaagat agccatgaac gtctaaactg tacaagaaca aaccaaatcc gataaatagg
     1321 ggcaggatag tcaaggcacc catacccact tgcaaggcct tttccccacc agttaaagga
     1381 cgataataat ctgggagccc ccagaaggaa actcgttttc tcactccttc ttgcatctgg
     1441 ttcaatacct cttcaaaaga agatttgagt tgaagccctc ttgcttgaat ccgtttttca
     1501 tcagaaactt tggctctacg ataaagacta tcagatacct tgtaatccgc aaacaaattg
     1561 gaaagagttt cttctttttt acctgaaaag gctagattta gacagtctct ctcaaagctt
     1621 gacaaaccat cttcttttac tagcctcaaa ccaactgcat ctccttctga aatgatagag
     1681 acattcccac ggtctatcac atctagcaag gtagcttgaa taagttgatc aaaggtgaat
     1741 tttccagctc ccttgaccaa gggactcact tcctccaagg aggtcgagta gactgcttct
     1801 gataaaacca taggctctaa ttccattggt ggttcataga gacgatgatt tttggcatat
     1861 ttgactgaag gagtggtctt tcttctataa ataaaataga agcagacact caataacaag
     1921 gagatggaaa ggatcgaagg gaggacccaa gtaagtagtt gtttactttg ctctttttct
     1981 ctaacaatcg agtcttctat cttattaaac tcttctaatc gattcccttt caatccctga
     2041 tccctagcgc tagcaaaatc ggtccgaggc caataggcat gcaactcaac tccacgctta
     2101 gccggaagat tgtctaaacg gatagtataa tcaaggttac tcttttcaat cgttccctct
     2161 ctaaaaagtt tccctgtatg gaaaaagagt ttttcagccc ccttgtctcc ccttacatga
     2221 aattcaaact tttcaataga ctctgaacta tctgtcagag gttgccaatt taattcagcg
     2281 atatcatcat aaaggaaaag taaatttttt aagttccaga cgaggtcaac ttcaactatg
     2341 tcgccctcct gacctggatt atagactctc acagtataac catccgcttc ttctgttact
     2401 tcgctagtca catctgctag ttctgcaccg tttttcgcgg cctgaatctt tggatgaggg
     2461 tcaatgtcaa acccgctagg catcttacca gcacgtccaa gtcccacgat ttggccctta
     2521 aagtcctcct caaactggta aactatcttc tgtctaaact ctgccgtatt atctgcatga
     2581 atatacaaat ccccttgata agagtttatc ttgaaatcaa tggcaaaaac agagagtgga
     2641 agaaggcaaa acaagcctaa caccagtaag aaaaaagttt ttttcatcaa ctaagccccc
     2701 tttttatctt tcttatcatt atagcatttt ttcgtaagta aaggcttact tatgttgaaa
     2761 aattacgcat ttttcgataa aataggagac agttattttt taatagatca gaataggaga
     2821 aatcatgaga aaaatatact tatctatttt cacaagtctc ttgctgatgc taggacttgt
     2881 caatgttgct caagccgatg aatatttacg catcgggatg gaagcagcat atgctccctt
     2941 taactggacc caggatgatg atagcaacgg agctgtcaaa atcgatggaa ccaaccagta
     3001 cgccaacgga tacgatgttc aaatcgccaa gaaaatcgct aaggacttag gtaaagaacc
     3061 tttggttgtt aaaaccaagt gggaaggtct agtccctgcc cttacttctg gtaagattga
     3121 catgattatc gcaggtatga gtccaactgc agaacgcaaa caagaaattg ccttttcgag
     3181 cagttactac actagcgaac cagttttgct tgtcaaaaaa gattctgcct acgcaagtgc
     3241 taaatctttg gatgacttta acggtgcaaa aatcacttct caacaagggg tctacctcta
     3301 cgacttgatt gcacaaatcc caggtgctaa aaaagaaaca gccatgggag acttcgctca
     3361 aatgcgacaa gctcttgagg ctggtgtcat tgatgcttat gtttctgaac gtccagaagc
     3421 actgactgct gaagctgcga actctaagtt caagatgatt caagtagaac ctggtttcaa
     3481 aactggggaa gaagatacag ctatcgccat tggacttcgt aaagatgaca ctcgtattag
     3541 ccaaatcaat gccagcattg aaaccatttc aaaggacgac caagttgccc tgatggatcg
     3601 tatgatcaag gaacaacctg ccgaagctac aacaactgaa gagactagca gtagtttctt
     3661 tagccaagtt gctaaaattc tttctgaaaa ctggcaacaa ctcttgcgtg gtgctggtat
     3721 cactctttta atctctatcg tcggaaccat cacaggtctc attattggac ttgccattgg
     3781 tgttttccgt actgctcctc tctctgaaaa caaagccatt tacggcctac aaaaactagt
     3841 cggctggatc ctcaacgtct acattgaaat tttccgtggt acgccaatga ttgttcaatc
     3901 gatggttatc tactatggaa ctgcccaagc tttcgggatc aaccttgacc gtacactggc
     3961 tgctatcttc atcgtttcaa tcaataccgg tgcctacatg actgaaatcg tccgtggtgg
     4021 tatcctagca gttgacaagg gacaatttga agctgcgact gctcttggta tgacccataa
     4081 ccagaccatg cgtaagattg tcctacctca ggtagtccgt aacatcctac ctgcaactgg
     4141 taatgaattt gtcatcaata tcaaagatac atctgtattg aacgttatct ctgttgtcga
     4201 actttatttc tcaggaaata ccgtggcaac acaaacctat caatacttcc agacatttac
     4261 aatcatcgcc gtgatttact ttgtcctcac cttcaccgta acacgtatcc tacgatttat
     4321 cgagcgccga atggacatgg atacctatac tacaggtgct aaccaaatgc aaacggagga
     4381 tttgaaataa tgacacaagc aatccttgaa attaaacacc tcaaaaaatc ctatggacaa
     4441 aacgaagtgc taaaagacat ttcactcact gtccacaagg gagaggtcat ctctatcatc
     4501 ggaagctctg gaagcggaaa atcgaccttc ctacgctcca ttaacctact tgaaacacca
     4561 actgatggac aaatccttta tcatggacaa aacgtcctcg aaaaaggcta tgacctcacg
     4621 caataccgtg aaaagttggg gatggttttc caatccttta acctctttga aaatctcaat
     4681 gttcttgaaa acacaatcgt cgctcagaca actgtcctta aacgagaacg cacagaagct
     4741 gaagaaattg ccaaagaaaa cctggaaaag gtcggcatgg gagaacgcta ctggcaagcg
     4801 aaaccaaaac aactctcagg tggtcaaaaa caacgtgtgg ccatcgctcg tgccctttca
     4861 atgaatcctg acgccattct ctttgatgaa ccaacatcag ctctcgatcc agaaatggtt
     4921 ggagaggtcc tcaaaatcat gcaagatctg gctcaggaag gcttgaccat gattgtcgta
     4981 acccatgaaa tggaatttgc ccgtgatgtc tctcaccgtg ttatctttat ggataagggt
     5041 gtgattgctg aagaaggtaa accagaagat ctcttcacta atcctaaaga agaccgtaca
     5101 aaagaattcc ttcaacgtta tctcata
//
LOCUS       DAVDWT010000068         4946 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVDWT010000068 DAVDWT010000000
VERSION     DAVDWT010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.068"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            79..1624
                     /locus_tag="VUW50_002057"
     rRNA            79..1624
                     /locus_tag="VUW50_002057"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1676..1748
                     /locus_tag="VUW50_002058"
     tRNA            1676..1748
                     /locus_tag="VUW50_002058"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1709..1711,aa:Ala,seq:tgc)
     gene            1873..4774
                     /locus_tag="VUW50_002059"
     rRNA            1873..4774
                     /locus_tag="VUW50_002059"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4851..>4946
                     /gene="rrf"
                     /locus_tag="VUW50_002060"
     rRNA            4851..>4946
                     /gene="rrf"
                     /locus_tag="VUW50_002060"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1380 a         1009 c         1461 g         1096 t
ORIGIN      
        1 ctacaacata agttgtagta ctgaacaatg aaaaaacaat aaatctgtca gtgacagaaa
       61 tgagtgagaa ctcaaacttt taatgagagt ttgatcctgg ctcaggacga acgctggcgg
      121 cgtgcctaat acatgcaagt agaacgctga aggaggagct tgcttctctg gatgagttgc
      181 gaacgggtga gtaacgcgta ggtaacctgc ctggtagcgg gggataacta ttggaaacga
      241 tagctaatac cgcataagag tggatgttgc atgacatttg cttaaaaggt gcacttgcat
      301 cactaccaga tggacctgcg ttgtattagc tagttggtgg ggtaagggct caccaaggcg
      361 acgatacata gccgacctga gagggtgatc ggccacactg ggactgagac acggcccaga
      421 ctcctacggg aggcagcagt agggaatctt cggcaatgga cggaagtctg accgagcaac
      481 gccgcgtgag tgaagaaggt tttcggatcg taaagctctg ttgtaagaga agaacgagtg
      541 tgagagtgga aagttcacac tgtgacggta tcttaccaga aagggacggc taactacgtg
      601 ccagcagccg cggtaatacg taggtcccga gcgttgtccg gatttattgg gcgtaaagcg
      661 agcgcaggcg gttagataag tctgaagtta aaggctgtgg cttaaccata gtaggctttg
      721 gaaactgttt aacttgagtg caagagggga gagtggaatt ccatgtgtag cggtgaaatg
      781 cgtagatata tggaggaaca ccggtggcga aagcggctct ctggcttgta actgacgctg
      841 aggctcgaaa gcgtggggag caaacaggat tagataccct ggtagtccac gctgtaaacg
      901 atgagtgcta ggtgttagac cctttccggg gtttagtgcc gtagctaacg cattaagcac
      961 tccgcctggg gagtacgacc gcaaggttga aactcaaagg aattgacggg ggcccgcaca
     1021 agcggtggag catgtggttt aattcgaagc aacgcgaaga accttaccag gtcttgacat
     1081 ccctctgacc gctctagaga tagagttttc cttcgggaca gaggtgacag gtggtgcatg
     1141 gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaaccccta
     1201 ttgttagttg ccatcattta gttgggcact ctagcgagac tgccggtaat aaaccggagg
     1261 aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc tgggctacac acgtgctaca
     1321 atggctggta caacgagtcg caagccggtg acggcaagct aatctcttaa agccagtctc
     1381 agttcggatt gtaggctgca actcgcctac atgaagtcgg aatcgctagt aatcgcggat
     1441 cagcacgccg cggtgaatac gttcccgggc cttgtacaca ccgcccgtca caccacgaga
     1501 gtttgtaaca cccgaagtcg gtgaggtaac cgtaaggagc cagccgccta aggtgggata
     1561 gatgattggg gtgaagtcgt aacaaggtag ccgtatcgga aggtgcggct ggatcacctc
     1621 ctttctaagg ataaggaact gcgcattggt cttgtttagt cttgagaggt cttgtggggc
     1681 cttagctcag ctgggagagc gcctgctttg cacgcaggag gtcagcggtt cgatcccgct
     1741 aggctccatt ggtgagagat caccaagtaa tgcacattga aaattgaata tctatatcaa
     1801 atagtaacaa gaaaataaac cgaaaacgct gtagtattaa taagagttta tgactgaaag
     1861 gtcagaaaat aaggttaagt taataagggc gcacggtgga tgccttggca ctaggagccg
     1921 acgaaggacg tgacaaacga cgatatgcct tgggtagctg taagtaagcg atgatccagg
     1981 gatttccgaa tgggggaacc caacaggtag tacctgttac ccacatctgt taaggatgtg
     2041 aggaggaaga cgcagtgaac tgaaacatct aagtagctgc aggaagagaa agcaaaagcg
     2101 attgccttag tagcggcgag cgaaacggca ggagggcaaa ccgaagagtt tactcttcgg
     2161 ggttgtagga ctgcaatgtg gactcaaaga ttatagaaga atgatttggg aagatcagcc
     2221 aaagagagta atagcctcgt atttaaaata gtctttgtac ttagcagtat cctgagtacg
     2281 gcgggacacg tgaaatcccg tcggaatctg ggaggaccat ctcccaaccc taaatactcc
     2341 ctagtgaccg atagtgaacc agtaccgtga gggaaaggtg aaaagcaccc cgggagggga
     2401 gtgaaataga acctgaaacc gtgtgcctac aacaagttcg agcccgttaa tgggtgagag
     2461 cgtgcctttt gtagaatgaa ccggcgagtt acgttatgat gcgaggttaa gttgaagaga
     2521 cggagccgta gggaaaccga gtctgaatag ggcgccttag tatcatgacg tagacccgaa
     2581 accatgtgac ctacccatga gcaggttgaa ggtgcggtaa gacgcactgg aggaccgaac
     2641 cagggcacgt tgaaaagtgc ttggatgact tgtgggtagc ggagaaattc caaacgaact
     2701 tggagatagc tggttctctc cgaaatagct ttagggctag cgtcgacatt agagattctt
     2761 ggaggtagag cactgtttgg gtgaggggtc catcccggat taccaatctc agataaactc
     2821 cgaatgccaa tgaattatgg tcggcagtca gactgcgagt gctaagatcc gtagtcgaaa
     2881 gggaaacagc ccagaccacc agctaaggtc ccaaaataat tgttaagtgg aaaaggatgt
     2941 ggggttgcac agacaactag gatgttagct tagaagcagc tattcattca aagagtgcgt
     3001 aatagctcac tagtcgagtg accctgcgcc gaaaatgtac cggggctaaa acaatttacc
     3061 gaagctgtgg atacctttat aggtatggta ggagagcgtt ctatgtgtga tgaaggtata
     3121 ccgtgaggag tgctggaacg catagaagtg agaatgccgg tatgagtagc gaaagacagg
     3181 tgagaatcct gtccaccgta agactaaggt ttccagggga aggctcgtcc gccctgggtt
     3241 agtcgggacc taaggagaga ccgaaaggtg tatccgatgg acaacaggtt gatattcctg
     3301 tactagagta tgtagtgatg gagggacgca gtaggctaac taaagcagac gattggaaga
     3361 gtctgtctaa gcagtgaggt gtgaattgag tcaaatgctt aattctataa cattgagctg
     3421 tgatggggag cgaagtttag tagcgaagtt agtgacgtca cactgccaag aaaagcttct
     3481 agcgtttaaa catactctac ccgtaccgca aaccgacaca ggtagtcgag gcgagtagcc
     3541 tcaggtgagc gagagaactc tcgttaagga actcggcaaa atgaccccgt aacttcggga
     3601 gaaggggtgc tgactttaag tcagccgcag tgaataggcc caagcaactg tttatcaaaa
     3661 acacagctct ctgctaaatc gtaagatgat gtataggggg tgacgcctgc ccggtgctgg
     3721 aaggttaaga ggagtgctta gcgtaagcga aggtatgaat tgaagcccca gtaaacggcg
     3781 gccgtaacta taacggtcct aaggtagcga aattccttgt cgggtaagtt ccgacccgca
     3841 cgaaaggcgt aatgatttgg gcactgtctc aacgagagac tcggtgaaat tttagtacct
     3901 gtgaagatgc aggttacccg cgacaggacg gaaagacccc atggagcttt actgcagttt
     3961 gatattgagt gtctgtacca catgtacagg ataggtagga gtctaagaga tcgggacgcc
     4021 agtttcgaag gagacgctgt tgggatacta cccttgtgtt atggccactc taacccagat
     4081 aggtgatccc tatcggagac agtgtctgac gggcagtttg actggggcgg tcgcctccta
     4141 aaaggtaacg gaggcgccca aaggttccct cagaatggtt ggaaatcatt cgcagagtgt
     4201 aaaggtataa gggagcttga ctgcgagagc tacaactcga gcagggacga aagtcgggct
     4261 tagtgatccg gtggttccgt atggaagggc catcgctcaa cggataaaag ctaccctggg
     4321 gataacaggc ttatctcccc caagagttca catcgacggg gaggtttggc acctcgatgt
     4381 cggctcgtcg catcctgggg ctgtagtcgg tcccaagggt tgggctgttc gcccattaaa
     4441 gcggcacgcg agctgggttc agaacgtcgt gagacagttc ggtccctatc cgtcgcgggc
     4501 gtaggaaatt tgagaggatc tgctcctagt acgagaggac cagagtggac ttaccgctgg
     4561 tgtaccagtt gtcttgccaa aggcatcgct gggtagctat gtagggaagg gataaacgct
     4621 gaaagcatct aagtgtgaaa cccacctcaa gatgagattt cccatgatta tatatcagta
     4681 agagccctga gagatgatca ggtagatagg ttagaagtgg aagtgtggcg acacatgtag
     4741 cggactaata ctaatagctc gaggacttat ccaaagtaac tgagaatatg aaagcgaacg
     4801 gttttcttga attgaataga tattcaattt tgagtaggta ttactcagag ttaagtgacg
     4861 atagcctagg agatacacct gtacccatgc cgaacacaga agttaagccc tagaacgccg
     4921 gaagtagttg ggggttgccc cctgtg
//
LOCUS       DAVDWT010000069         4884 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVDWT010000069 DAVDWT010000000
VERSION     DAVDWT010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.069"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(685..858)
                     /locus_tag="VUW50_002061"
     CDS             complement(685..858)
                     /locus_tag="VUW50_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HET7983881.1"
                     /translation="MNIKKRVLSAGLTFASALLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYADNIVN"
     gene            complement(1017..2624)
                     /locus_tag="VUW50_002062"
     CDS             complement(1017..2624)
                     /locus_tag="VUW50_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HET7983882.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGAKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2777..4261
                     /locus_tag="VUW50_002063"
     CDS             2777..4261
                     /locus_tag="VUW50_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HET7983883.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTNMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            4356..4838
                     /locus_tag="VUW50_002064"
     CDS             4356..4838
                     /locus_tag="VUW50_002064"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HET7983884.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
BASE COUNT         1388 a         1132 c          929 g         1435 t
ORIGIN      
        1 agcatatttt cgcgtgataa tatcttatct agcagttttg acatgtgcgt attcctttct
       61 tgttttggtg cctgagggac atcttatatt ggtgaacctt catctagtca atacgtgaca
      121 gactccagtt ctatcagacc tctcgtttta cagacacaag tgaagtagga ttacttcaat
      181 tagttgctca ccccttcgct ccacttccac tacagaagtt tcctcactac tatgggttcg
      241 gctgacttct catgattcgt tgttactacg ctttcagcgc tcatgagacc tcacgggata
      301 aatcgtacat ctttcctcgt ttatacaaga tatgaggacg ttaaaaggtt acagtgaaaa
      361 taggaaatct tagtccgaat tcaattactc tcgtgattta cgcatacagg ttacgactac
      421 ctttttggac tttagagttt gaggccccct tatccgctat atacgcctta ctatcacgtt
      481 cctgttcgta gagccacgat ttcgctatcc cttcctctcc ctgctacctc acgatagtca
      541 ggcttgggaa tcgctattgg gttcactggt agcaggtgcc caccgaggac ttacacctca
      601 gatatacgac atgcccgtcg tacatcaaaa aggtattacc aagttgcaat accttttaca
      661 aggctctttt gtcttgttct tgtttcaatt gactatatta tcagcgtaat agtctaacag
      721 gtaattaaat gtagttggat ttccactaaa ggttgatgag taagtttttg tatctgaacc
      781 tgattggccg caagcagcta aaagcaaagc agatgcaaaa gtcagacctg cactaaggac
      841 acgcttcttt atgttcatct tctttctcct taatagtgag aatttgtaaa gttaattgaa
      901 tttcgagaat gaaagtttat aaaccttggt tataaaaaac aaaggatttc tgtcttttat
      961 acagtcctcc cttattttta tacgatttca attttaaatt tttctgcaaa aaatatttat
     1021 agtaattcca cacagaaagc atcccatgga actaagattt gtttttcaaa gacttcttga
     1081 gctagggtgt tttcaatcaa gacagatttg acttttcctt ctactgtcaa gtcttgctct
     1141 tcattggaca agttagccac aactaggaag cgacggtcgc catccttacg tatataagca
     1201 aagaccttat cagccgtatc aagcaattca aagtcagctc gaattagcca gctattctcc
     1261 ttgcgaattt ggaccagttt ctgataggta tagaaaatag aatctggatt tgccagcgct
     1321 tcttggacgt tgatcatctc gtaatttgga ttaaccgcca accaaggttg acctgttgag
     1381 aaaccagcgt ttttgctctc atcccattgc ataggggtac gggcattgtc acgtccaata
     1441 acacggatac tgtccatgat ttcttcaatc ggaacacctt tttcaagagc ctcacgcgca
     1501 tagttgagag attcaatatc ttctacttga tccagtgttt caaacggata gttggtcatc
     1561 ccaatctcct caccttggta gatataagga gttcctctca tgagatgaag taagattgca
     1621 aaggctttgg cagatttttc gcggtattct tggtcatttc cccagattga gacaatacga
     1681 gggaggtcat ggttgttcca gaagagggaa ttccagccgt cctcaactcc taactctgtc
     1741 tgccatttgt tgaagatttc ttttaactta gcgatattca gttctttttg atagtgccat
     1801 ttaggctgac cttcctgata ctgaagaccg atatgttcaa actggaagac catagacaat
     1861 tcttgcccct ttggatcaga gtagagcttg gcaatctctg gcgttgctcc ccaagtctcc
     1921 cctactgtca agagatcctt agctccaaag gtcgcctgat tcatttcctt gagataggga
     1981 tggagcatag gaccattatt gactaccttc tcgtcaggaa ttttgccaat catgtcaata
     2041 acatccatac ggaaaccacc aataccttta tcaatccaga agttcatcat ctcataaatt
     2101 ttctggcgaa gtttctcatt ttcccagttg agatccggct gtttcttgct gaaaaagtgg
     2161 agatagtatt gaccagattt ttcatcgtat tcccaagcag atccactaaa aatagaatct
     2221 aaatcgttag gctcatcccg ccagatatag tagtctcgct cagggctatt aggattttca
     2281 caggcctcta caaaccaggc atgctcatcc gaggtatgat tgaccaccaa gtccatgatg
     2341 atacggatat cacgcttctt agcttccgca atcagttgat ccatatcctc catggttcca
     2401 aaaatcgttg caatcgcttg ataatcagca atatcgtagc cattatcatc catagggctg
     2461 tcataaacgg gagaaagcca aatcgctgtg attcctagct tggctagata gtccaactta
     2521 ctggtaatac ctggcaaatc accaactcca tctccattac tatccataaa actctttgga
     2581 tagacttgat agactacggc attgtgccac catttttctt gcatctgttt acctcattat
     2641 tttaataatc tatagtctat gatagcactt tttaaaattt tgtgcaagcg tttgcttaat
     2701 ctttttaatt tcttttttag ctccgtagtt tcctaacttc caatttttta ttataataga
     2761 gttcagaggt aaaaaaatga aaaaacaagc ttttagttct gaacagtatt tgaatttaca
     2821 acgcgaccat attttggagc gcattaacca atttgacggc aagctctact tagagtttgg
     2881 cggtaaaatg ttagaagatt tccacgctgc tcgtgtcctt cctggttatg aacctgacaa
     2941 caaaatcaag ctcttgcaag aattgaaaga gcaggttgag gttgtgattg ccattaatgc
     3001 tagcaacatt gaacattcca aagcacgtgg cgacttaggc atttcttatg accaagaagt
     3061 tcttcgtttg attgacaaat tcaatgaatt agggattttt gttggttccg ttgtcattac
     3121 acaatacgct ggccaacccg ctgcagatgc cttccgcaac caacttgaga aaaacggaat
     3181 tgattcttat cttcattatc caatcaaagg atatccgacg aatatggatc acatcatttc
     3241 cccagaaggc atgggcaaaa acgactacat caaaaccagt cgcaacttga ttgtcgtaac
     3301 cgctcctgga cccggttctg gaaaattggc aacgtgtatg tccaatatgt accacgacca
     3361 aatcaatggc atcaaatctg gctacgctaa atttgaaacc ttccctgttt ggaatcttcc
     3421 ccttcatcat ccagttaact tggcttatga agctgccaca gctgaccttg atgatgtcaa
     3481 catgattgac cccttccatc ttcaaaccta tggagaaacc actgtcaact acaaccgtga
     3541 tatcgaaatc ttcccagtgc tcaagcgcat gttggaacgt attctcggaa aatcaccata
     3601 cgcttcaccg acagatatgg gtgtcaacat ggttggtttc gctattacag atgacgaggc
     3661 tgctgtcgaa gcttctaaac aagaaatcat ccgccgttac tatcaaacag ttcttgactt
     3721 caaagctgaa aaagttggcg aagctgccgt caagaaaatt gagttgctca tgaacgacct
     3781 cggtatcaca cctgcagacc gtaaggttgc tgtcgttgcg cgccaaaaag cagaagaaac
     3841 tggtggacca gccctagcct ttgaattgcc aaatggggaa atcgtcactg gtaagaactc
     3901 agaactcttt ggccctacag ccgctgcctt gatcaacgcc atcaaaaaat cagctgacat
     3961 cgctaaagaa gtaaaattaa tcgagccgga agttgttaag ccaatccaag gtcttaaaat
     4021 cgatcatctc ggtagccgca atccacgcct tcattcaaat gaaatcctga ttgcacttgc
     4081 tatcacagct acagaaaatc ctgatgctgc ccgcgctatg gaagaactcg gcaacctcaa
     4141 aggaagcgaa gcccactcaa ccatcatctt gactgatgaa gacaagaatg tccttcgtaa
     4201 actgggtatc aacgtaacct ttgacccata ctaccaatac gaccgcttat atcgtaagta
     4261 aagatttcaa cctctccagc ctcggagagg ttttctctct gtctcaggag caacctttat
     4321 gatataatga gagaagaaaa ctgaaaggat ttaccatgtc aaaagaagtt attgtcgaaa
     4381 gttttgaact tgaccacacc attgtcaaag caccctatgt tcgcttgatt ggggaagaaa
     4441 caggaccaaa aggagacatc atctccaatt atgatattcg cttggtgcaa ccaaacgaag
     4501 actctatccc tactgccggc cttcacacta tcgagcacct cttagccaaa ctcatccgta
     4561 cccgcattga cggcatgatt gactgttcac catttggttg ccgcacaggc ttccacatga
     4621 ttatgtgggg acgtcacacc agtgctaaaa tcgcagctgt tatcaaggat tcgctcaagg
     4681 aaatcgctga aactactact tgggaagatg tcccagggac aaccatcgaa tcttgcggaa
     4741 actacaagga ccacagcctc ttctctgcta aagaatgggc aaaactcatc ttggaacaag
     4801 ggatttcaga tgatgccttt gaacgtcatg tgatttaaaa tcgagtaggg gataatctct
     4861 agcccctctc acaccaccgt acgt
//
LOCUS       DAVDWT010000070         4384 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVDWT010000070 DAVDWT010000000
VERSION     DAVDWT010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.070"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(138..1427)
                     /locus_tag="VUW50_002065"
     CDS             complement(138..1427)
                     /locus_tag="VUW50_002065"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET7983885.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(1505..2752)
                     /locus_tag="VUW50_002066"
     CDS             complement(1505..2752)
                     /locus_tag="VUW50_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET7983886.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGVSIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVSVIISSLTGNDYFGKQSLPRLMMWETVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(2943..3793)
                     /gene="rgg"
                     /locus_tag="VUW50_002067"
                     /pseudo
     CDS             complement(2943..3793)
                     /gene="rgg"
                     /locus_tag="VUW50_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398326.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes.; frameshifted;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
     gene            3917..4258
                     /locus_tag="VUW50_002068"
     CDS             3917..4258
                     /locus_tag="VUW50_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET7983887.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
BASE COUNT         1384 a          926 c          850 g         1224 t
ORIGIN      
        1 atgttttgag ctgacttcgt cagttctatc cacaacctca aaaccatgtt ttgagctaac
       61 ttcgtcagtt ctatccacaa cctcaaaaca gtgttttgag caacctgcgg ctagcttcct
      121 agtttgctct ttgattttta ttgagtataa aatcctagtt tttcaaagat ttctgagaag
      181 ttttggctga ttgtctcaag tgacacttgc acttcttctc gggtttggtt gttcttgacc
      241 gtcacttgtc cgctttcgac ttcgctctct cctagggtga tgagggtctt agccgcaaag
      301 acatcggctg acttgaactg agcttttagt ttacggttga ggtaatcacg ctctgctttg
      361 aaaccttgtt ggcgaagagc ctgtaccaat tccaaggcct tgatatttgc cccttcgccc
      421 aagactgcga tatagacatc tagggcgttt tcgataggga gggtcacacc ttgcttttca
      481 aggatgagaa gcaggcgctc tacaccaagt ccaaaaccaa atccagcagt ttcagggcct
      541 ccaaagtaag caaccaaacc atcgtagcga ccacccgcac agacggtcag gtcattgccc
      601 tcaatctctg tgataaactc gaaaatggtg tggttgtagt agtccagacc acgcaccata
      661 ttggtatcga tgatgtaatc tactccaaga ttttccaaca tctgacgcac agcatcaaaa
      721 tgagcttggc tttcttcatc aagaaagtcc aagatagacg gcgcattctc tactgccacc
      781 ttgtcttctt tttccttaga gtccaagaca cgaagaggat tttcctccaa gcgacgttgg
      841 ctatccttag acaaggtctc cttgagcggt gtcaaatagt caatcaaggc ttggcggtag
      901 gctgcacggc tctcaggatt tccaagagtg ttgaggtgca atttgacacc ttgaataccg
      961 atttccttca aaaaatgggc tgccatagcg attgtttcca catcggtagc tggattgcta
     1021 gagccaaaac actcaacacc aatctggtgg aattggcgca agcgccctgc ctgtggacgc
     1081 tcataacgga acataggtcc catgtagtag aacttgcttg gcttttgcac ttctggggcg
     1141 aaaagtttat tttccacata ggaacggaca acgggtgcag ttccttctgg acggagggta
     1201 atatgacggt cacccttgtc ataaaaatcg tacatttcct tggttacgat atccgttgta
     1261 tctccgacag agcgactgat aacctcgtaa tgctcaaaaa taggcgtgcg cacttctgca
     1321 tagttgtagc gtttgaaaat ctcacgggca aagccctcaa cgtactgcca cttagcagac
     1381 tcagcaggta aaatatcctg cgttcctttt ggtttttgta atttcatagg gaatcctctt
     1441 taaacttaat agtcttattt taccataaat agagggatta aaacagtaag aaaaaaatta
     1501 ggatttagat atcatttttg agattaagaa ttgtcaaaaa aatagctagc aaggaaagac
     1561 caacaaatag catccaagtc aactgtatat tccatacggc tactagtgaa aaacaagctg
     1621 ttcccacagg tatggataag gtaaacaata gacctaaaaa attactagta cgagctagaa
     1681 cctctggagc tagatttttc atgagcatgg cactaatctt tggttgaact ttaccagaca
     1741 catacagagt aaagaagaga aatagcaaac caagcacgac ttgattgaat aaattagcca
     1801 aaccaactag actaagtcct acggtctccc acatcatcaa tctaggcaag gactgcttcc
     1861 caaaataatc attgcccgta aggctactga tgatgactga tactaaaaca cagaattgat
     1921 tgataaatag tgcctctgta taagaaaaat tcaagagaga atggctcaaa aagaagatat
     1981 tataaattcc acccaaagcg ccacccaagg aattaataag caagacagca aagagcataa
     2041 aaccaaagtt tttctgtcca cttttaagaa aaacgagacg taaatttcgg taaattgtta
     2101 ggaactggtc tttgatagaa agcttctcat tttttaagtt ttcaccatca gcagatgaca
     2161 ttgacaggct caatttgctt tttcctaaaa agaggatagt ggctgatact aggaaaaagc
     2221 aggcattgat tcccgcaacg agagaaaaat tgttgaccga tagagctaag agccagactc
     2281 cgaaagcttg accaccaata gctgaaatat aggtgatgaa ctgtgaaaaa gaataagcct
     2341 ccatcagatc atcttcagct actttttcct taataagagg catacgcagg ccacctgcaa
     2401 aatcactgat gatatcacta atgacattga tcaaacacag gctagaaaag gcaaagagac
     2461 tagcttgctg aacaactagg gctgctagaa aaaatagaac cgcctgaaac aaaccgctat
     2521 agaccatcca tttgaccttg tccctcgtgt aatctgcccg aatccctgca aaaactgtaa
     2581 agagggtcgg aagaatcatg acaatattcg ccatagcaac agcaaaagat gcttgtgaca
     2641 aggtcgatgc atagacgata aagaccaggt tgaaaatcga aacaccaaaa gcattgaaga
     2701 agcgtgataa agtcaaaatt ctataagctg gatttctaaa caatagtttc atagataatc
     2761 tccctccttg ttctaagttc tacatcaaga atagctttag tatagcactt atgaatttgc
     2821 gaaacgattc ttttgaatat atgcaacaaa caaaatgagc aaaaaaacga tagaactttt
     2881 gtaatgacct gagagagggc aacagttcta tcgcttcaaa ttttttcttg gtttgcagat
     2941 attcaagaat cgggagtttt tctatagtat tcggcagatt tattacagcc aagcatctca
     3001 aaaatacgga cagcatcctc catctttttc tggccttcct tgactctacc ttgcttgcta
     3061 tcaaggagac cttctgccca cagataaaca attcggaaat aggtctcatt ttccttgtag
     3121 aaatgctctt cgataacacg tttaaaataa taggcattgg taaattcttc acactcaata
     3181 ctagctaaaa agccattcaa tagtatagta tgaaaaaggt ttcgattgcc agacatttcc
     3241 attagaaaat cagatttacg taccatttct cgtacatatc tagtaaaaag agaaacagat
     3301 aaaaatggag aactgactga aaataaattg agttcataga ttccccagat ctcggtagaa
     3361 aacaaataat catgaaggac ttttccttcc tctgctgtta agtctaccct ttcatctatg
     3421 ctcttcatat aagacttgat aataatggca tttagaatat gtttctgttt gttgtgagaa
     3481 tgggcatgct tttatactcc ctgcgatata agtcctcaag aggtgctata ttctttggtt
     3541 ccaagacatc tgtaatttct tttctcaact cagaatctgt atcatactgg aaacctcttg
     3601 ccagaaagag gatctcctcc acactggcag atatattttc cagagcaaat agaaactttt
     3661 ccaccgaaag ctcactctga cctgtttcaa aacgggacaa catagacggc gaaaattgtc
     3721 ctccggttgc ttgtctcagt gagatatttc ttgactctcg taattgtcta aagacttttc
     3781 caatctgctc catagacttc cccttgattc cgtattttct tcattttatc atatttttca
     3841 gaaaattcat caaaaacttg ccaaattgtc agaattatga gaaaatagag gatatttatc
     3901 acgtggaggg actgctatga gagacgatat caaaatcaat gaccgtgctt tggccttgca
     3961 agaccaaatt atcgaaaaac tagagaaagt ttttgataca gatgtggaat tggatgttta
     4021 caatctaggt ctgatttatg aaatcaatct ggatgaaacg gggctctgca agattgtcat
     4081 gaccttcacc gatactgcct gtgattgcgc cgaaagcctg cctattgaaa tcgtggcagg
     4141 tctgaaacaa atcgagggta tcaaagatat caaggttgaa gttacctggt cgcctgcttg
     4201 gaaaatcaca cgaatcagtc gctatggccg tattgccctt ggactaccac ctcgttaagc
     4261 agaccaatca cttttaaaga tgaaaaaagc aagccgaaaa tggcttgctt tttgatactc
     4321 aatgaaaatc aaagggcaaa ctagaaaact agccgcaggt tgctcaaaac actgttttga
     4381 agtt
//
LOCUS       DAVDWT010000071         4209 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVDWT010000071 DAVDWT010000000
VERSION     DAVDWT010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.071"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(75..911)
                     /locus_tag="VUW50_002069"
     CDS             complement(75..911)
                     /locus_tag="VUW50_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET7983888.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            1037..3232
                     /locus_tag="VUW50_002070"
     CDS             1037..3232
                     /locus_tag="VUW50_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET7983889.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            3285..3437
                     /gene="rpmG"
                     /locus_tag="VUW50_002071"
     CDS             3285..3437
                     /gene="rpmG"
                     /locus_tag="VUW50_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET7983890.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            3447..3623
                     /gene="secE"
                     /locus_tag="VUW50_002072"
     CDS             3447..3623
                     /gene="secE"
                     /locus_tag="VUW50_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET7983891.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            3678..>4209
                     /gene="nusG"
                     /locus_tag="VUW50_002073"
     CDS             3678..>4209
                     /gene="nusG"
                     /locus_tag="VUW50_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET7983892.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         1324 a          733 c          916 g         1236 t
ORIGIN      
        1 aaaataggct ccataatatc tatagtggat ttacccacta caaatattat agagccgaaa
       61 aatggatgac acgatcatcc attctttttt aattcttttt caaatgtatt tgaaagggta
      121 gtgaaagtta gcttctctaa agtaagtggg tgggtaaagg aaagtcggaa ggcatgaagc
      181 ataagccgac ttgtctttga tttactatta tagagagggt cgcccaggat aggaagatta
      241 tgatgcgaaa ggtgcacacg aatctgatgg gttcgccctg tctttagctt gcaacgagcc
      301 aaggaagtct tgtttgagaa ttgctttaat ctgcttacat gcgtttcagc atattgccca
      361 ttttttgcat caactattct ttttctacga tcatggcgat cacgtccaat tttgtctctg
      421 aaaacaagtt cttttctgtt gatatttcca tcaactagag cccaatattc tctagaaatc
      481 tcttttttct ccaataagcg attgagaatg ggcaggataa aaggattttt ggcaaagaga
      541 actaagccac tggtttccat gtccagacga tgaacgacat agcaggtttg gccaacataa
      601 gtactgacat ggttaagaag ggcaatttcg tttggttgat taccatgtgt tttcatcccc
      661 tctggtttgt ttacaataat caagtgttga tcttgataaa cttcctgcac taagtctggg
      721 ttgccccaag ggatcgtctt ttgggaataa tcttcctcgt caaaagtcaa ctggcaaaca
      781 tctccaggat ttacgatttc gttccagtgg acttcttctt gatttatcaa aatatgtttt
      841 ttgattctca aaaaatgacg gatttttcta gggatgagga gttgttcctc aagtaattgc
      901 tttaccgtca tttgaggtag agaggcgggt aatgtaaatg tgaattgcat acagatattg
      961 taacaaaaaa agccctattt ggataggaaa tagctaaatt cttgtcttcc tatgatgaag
     1021 atgataaaat aaacgcatga aattagataa attatttgag aaatttcttt ctctttttaa
     1081 aaaagaaaca agtgaactag aggactctga ttctactatc ttgcgtcgct ctcgtagtga
     1141 tcgaaaaaaa ttagcccaag taggtccgat tcgaaaattc tggcgtcgtt atcatctaac
     1201 aaagattatc cttatactag gtttgagtgc aggcttgcta gttggaatct atttgtttgc
     1261 tgtagccaag tcaaccaatg tcaatgattt gcaaaatgcc ttgaaaactc ggactcttat
     1321 ttttgaccgt gaagaaaaag aggctggtgc cttgtctggt caaaagggaa cctatgttga
     1381 gctgactgac atcagtaaaa acttgcagaa tgctgttatt gcgacagaag accgttcttt
     1441 ctataaaaat gacgggatta actatggccg tttcttcttg gctattgtca ctgctggacg
     1501 ttcaggtggt ggctctacca ttacccaaca gctggctaaa aacgcctatt tatcgcagga
     1561 tcaaactgtt gagagaaaag cgaaagaatt tttccttgcc ttagaattaa gcaaaaaata
     1621 tagtaaggag caaattctaa ccatgtacct taacaacgct tattttggaa atggtgtgtg
     1681 gggtgtagaa gatgcgagta agaaatactt tggagtttct gcatcagaag tgagtctgga
     1741 tcaagctgcg actctggcag ggatgctcaa ggggccggaa ctgtataatc ccttgaattc
     1801 cgtagaagat tctactaatc ggcgcgatac tgtcttgcag aatatggttg cagcaggata
     1861 tattgataaa aaccaagaaa ccgaagctgc tgaagttgat atgacttcgc aattgcacga
     1921 taagtatgaa ggaaaaatct cagattaccg ttacccctct tattttgatg cggtggttaa
     1981 tgaagctgtt tccaagtata atctaacaga ggaagagatt gtcaataatg gctaccgcat
     2041 ttacacagag ctggaccaaa actaccaagc aaatatgcag attgtttatg aaaacacatc
     2101 gctatttccg agggcagagg atggaacgtt tgctcaatca ggaagtgtag ctctcgaacc
     2161 gaaaacaggg ggagttcgtg gagttgtcgg tcaagttgct gacaatgata aaactggatt
     2221 ccggaatttc aactatgcaa cccaatcaaa gcgtagtcct ggttctacaa ttaagccttt
     2281 agttgtttat acaccagcag ttgaagcagg ctgggctttg aataagcagt tggataacca
     2341 taccatgcag tatgacagct ataaggttga taactatgca gggatcaaaa cgagtcgaga
     2401 agttcctatg tatcaagcct tggcagaatc gcttaatcta cctgctgttg ccactgttaa
     2461 tgatttgggt gtcgacaagg cttttgaggc aggcgaaaaa ttcggactca acatggaaaa
     2521 ggtcgaccgt gttcttggtg tcgccttggg aagcggtgtt gaaaccaacc ctcttcaaat
     2581 ggctcaagca tacgctgcct ttgcaaatga aggtttaatg cctgaagctc attttattag
     2641 tagaattgaa aatgctagtg gacaagttat tgcgagtcat aaaaattcac aaaaacgggt
     2701 gattgataag tctgtagctg acaagatgac cagtatgatg ttggggactt tcaccaacgg
     2761 taccggtatt agttcatcgc ctgcagacta tattatggca gggaaaactg gaacaactga
     2821 agcagttttc aatccggagt acacaagtga ccagtgggta attggttata ctccggatgt
     2881 agtgattagc cactggcttg gctttcctac cactgatgaa aatcactatc tagctggctc
     2941 tacttcaaac ggtgcagctc atgtctttag aaacattgcc aataccattt taccctatac
     3001 gccaggaagt acctttacgg ttgaaaatgc ttataagcaa aatggaattg caccagccaa
     3061 tacaaaaaga caagtacaaa ccaatgataa tagccagaca gatgataatt tgtctgatat
     3121 tcgagggcgt gcgcaaagtc tagtagatga ggctagccgg gctatctcag atgcgaagat
     3181 taaggaaaag gctcaaacaa tatgggattc gatagtcaat ctatttcgtt aagatgcttg
     3241 tcaaagccta gctttcttgt tataatggat aagatggagg cgttatggca ctaaaaaaag
     3301 caagcctagc ttgtgcggtt tgtggttcga gaaactattc aatcaagatc agcggaaacc
     3361 ccaagcctac acgactagaa gtaaataaat tttgtaagca ttgtggcaag tacactacac
     3421 acagagaaac gagataggag agagcgatgc gttttattgg agatattttt agacttctta
     3481 aagacacaac atggccaact cgcaaggaaa gctggagaga ttttcgttct atcatggaat
     3541 acacagcttt ctttgtagta attatttaca tttttgacca gttgattgtt tcaggtttga
     3601 ttcgatttat taacattttt tagaagatta gtggagttaa ttacactaga aatcttctat
     3661 ttatgaaagg aaatatcatg gatagttttg ataaaggatg gtttgtttta caaacttatt
     3721 ctggttatga aaataaggtg aaagaaaatc tattacaacg tgcacaaacc tacaatatgt
     3781 tggataatat tctacgcgtt gaaattccaa cacaaacagt gcaagttgaa aaaaatggaa
     3841 agagaaaaga agtagaagaa aatcgctttc caggttatgt tcttgtagaa atggtcatga
     3901 cagatgaagc ttggtttgtt gttcgaaaca caccaaatgt tacaggattt gtcggatcac
     3961 acgggaacag atcaaaacca actccattat tggaacaaga aattcgtgac attttggtat
     4021 ctatgggaca aactgttcaa gaatttgatt tcgatgttga gattggtcaa actgtacgta
     4081 ttattgatgg tgcttttgca gactacactg gtaagattac agaaattgat aataacaaag
     4141 tgaaaatgat tatctctatg tttggtaatg acacagttgc agaagtaaac ctaaaccaaa
     4201 tcgcagaat
//
LOCUS       DAVDWT010000072         4011 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVDWT010000072 DAVDWT010000000
VERSION     DAVDWT010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.072"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(147..1208)
                     /gene="dinB"
                     /locus_tag="VUW50_002074"
     CDS             complement(147..1208)
                     /gene="dinB"
                     /locus_tag="VUW50_002074"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET7983893.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITLTGF"
     gene            1556..3880
                     /gene="pflB"
                     /locus_tag="VUW50_002075"
     CDS             1556..3880
                     /gene="pflB"
                     /locus_tag="VUW50_002075"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HET7983894.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT         1205 a          846 c          779 g         1181 t
ORIGIN      
        1 gaaaaacctt gaaggggcaa ctcttcaagg ttttatactc ttagaaaatc tcttcaaacc
       61 gcgtcagctt tatctgcaac ctcaaaacag tgttttgagc agcctgcggc tagcttccta
      121 gtttgctctt tgattttcat tgagctttaa aatccagtca gggtaatccc caataggcgg
      181 acacctcttt ctttctcgct taattcttca tagagttgca gggctatttg gcttatctga
      241 ctagcatctt gtgttttttg agcaagactt tttcgtttgg taagagttga aaagtcctcg
      301 tagcggattt tcaaaatgac aatttttcca gctttttctt gttgatgtag attgagagcg
      361 actttttctg atagaagagt cagctctttt ttgatatctt cctcagcaag gagaatcttc
      421 ccgtaggttt tctccttgcc gattgattta cggatgcgat tggatttgac tggagagttg
      481 tgaatgccac gagcctttcg atacagatca tagcctagtc taccaaaacg gtctattagg
      541 gttacctcag gaacttcaag taaatcagca ccagtaaaaa cgcccatttg atgaagacgt
      601 tctactgtct tttttcctac tccatgaaat ttggaaatat ccatttgttt gagaaaatcc
      661 tcagcctgtt caggtagaat cactgtcaaa ccatgtggtt tttgataatc actcgccatt
      721 ttagctaaga atttgttgta agaaacgcct gcggaagcag ttagatggag ttcttgccag
      781 atatcttttt gaatgaggcg agcaattttg accgctgact tgataccgag tttattttct
      841 gtcacatcca aataggcttc gtcaatgctc atgggttcaa tcaaatctgt atagcgctta
      901 aaaatagctc gaatctggag tcccacagac ttgtatttct cataattccc tgagataaag
      961 acagcctggg gacaacgttc ataagcttcc ttggaactca tggcagaatg gacaccaaaa
     1021 gctcttgcct cataactaca ggtagaaacg actccccgtc cacctgtttg ccgagggtcg
     1081 cttccaataa tgacaggttt tcctctgagt ttaggattat ccctgatttc cactgcagca
     1141 aaaaaggcat ccatgtcaat atggatgatt tttcttgaca aatcatttaa caaaggaaaa
     1201 atcaacatgc ctagcacctt tttatactct tcgaaaatct cttcaaacca cgtcagctct
     1261 atctgcaacc tcaaaacagt gttttgagca atctgcggct agcttcctag tttgcttttc
     1321 gatttccatt gagtgttact gcttattttc ttttattata cccttttttc tgaaaaaaag
     1381 aaaaaaggac tttatttttt caaaaatata atacagtttg aaataaaata tagactgttt
     1441 tagaaaagaa agtgtaaaaa tagggaattt tcacttgttg aaatcggtta ctatatggta
     1501 tacttgtctt atgaatgtaa cagatgactg ttactagaaa aaagaggaca ttaatatggt
     1561 tgttaagaca gttgttgaag cacaagatat ttttgacaaa gcttgggaag gcttcaaagg
     1621 cgtagattgg aaagaaaaag caagtgtatc acgatttgta caagctaact acacacctta
     1681 tgatggagac gaaagcttcc ttgcaggacc aacagagcgt tcacttcaca tcaagaaaat
     1741 tgtagaagaa actaaagcac actacgaaga aactcgtttc ccaatggaca ctcgtccaac
     1801 atctatcgct gatatccctg ctggatttat cgacaaagaa aatgaagtta tctttggtat
     1861 ccaaaatgat gaactcttca aattgaactt catgccaaaa ggtggtatcc gtatggctga
     1921 aactacttta aaagaaaatg gatacgaacc agacccagct gttcacgaaa tcttcactaa
     1981 atatgtaaca acagttaacg acggtatttt ccgtgcctac acttcaaata ttcgtcgcgc
     2041 tcgtcatgca cacactgtaa ctggtcttcc agatgcatac tcacgcggac gtatcatcgg
     2101 tgtttacgca cgtcttgctc tttacggtgc agactacttg atgcaagaaa aagtaaacga
     2161 ctggaatgca atcaaagaaa tcgatgaaga aacaatccgt cttcgtgaag aagtaaacct
     2221 tcaataccaa gcattgcaac aagttgttcg cctgggtgac ctttacgggg ttgatgttcg
     2281 caaaccagcg atgaacgtga aagaagcaat ccaatgggtt aacattgctt tcatggctgt
     2341 ctgccgtgtg attaacggtg ctgctacatc tctaggtcgt gtaccaatcg tattggacat
     2401 ctttgcagaa cgtgaccttg ctcgtggtac atttactgaa tcagaaatcc aagaattcgt
     2461 tgatgatttc gttatgaaac ttcgtacagt taaatttgct cgtacaaaag cttatgacca
     2521 attgtactca ggtgacccaa cctttatcac aacttctatg gctggtatgg gtaacgacgg
     2581 tcgtcaccgt gttactaaga tggactaccg tttcttgaac acacttgaca acatcggtaa
     2641 ctcaccagaa ccaaacttga cagttctttg gactgacaaa ttgccataca acttccgtcg
     2701 ctactgtatg cacatgagcc acaaacactc ttctatccaa tacgaaggtg taacaacaat
     2761 ggctaaagac ggatatggtg aaatgagctg tatctcatgc tgtgtgtctc cacttgatcc
     2821 agaaaatgaa gaacaacgcc acaacatcca gtacttcggt gctcgtgtaa acgttcttaa
     2881 agcccttctt actggtttga atggtggtta cgacgatgtt cacaaagact acaaagtatt
     2941 tgatatcgaa ccaatccgtg acgaagttct tgaattcgaa tcagttaaag cgaactttga
     3001 aaaatctctt gactggttga ctgacactta cgtagatgcc ttgaacatca tccactacat
     3061 gactgatagg tacaactacg aagctgttca aatggccttc ttgccaacta aacaacgtgc
     3121 caacatggga ttcggtatct gtggatttgc taacactgtt gatacattgt cagctatcaa
     3181 atacgctaca gttaaaccaa tccgtgacga agatggctac atctacgatt acgaaacaat
     3241 cggtgactac ccacgctggg gtgaagatga cccacgttca aacgaattgg cagaatggtt
     3301 gatcgaagct tacacaactc gtctacgtag ccacaaacta tacaaagacg cagaagctac
     3361 agtatcactt ttgacaatca catctaacgt tgcttactct aaacaaactg gtaactcacc
     3421 agttcacaaa ggtgtatacc tcaacgaaga tggttctgtg aacttgtcta aacttgaatt
     3481 cttctcacca ggtgctaacc catctaacaa agctaaaggt ggttggttgc aaaacttgaa
     3541 ctcactttct agccttgact ttagttatgc agctgacggt atctcattga ctacacaagt
     3601 atcacctcgc gctcttggta agactcgtga tgaacaagtt gataacttgg taacaatcct
     3661 tgatggttac ttcgaaaacg gtggacaaca cgttaacttg aacgttatgg acttgaacga
     3721 tgtttacgaa aaaatcatgt caggcgaaga cgttatcgta cgtatctctg gatactgtgt
     3781 aaacactaaa tacctcactc cagaacaaaa aactgaattg acacaacgtg tcttccacga
     3841 agttctttca atggatgacg ccttggatgc attgagctaa tcaagttcgg ctctttgtca
     3901 actgcagtgg gttgaagtcg gctaagctcg agaaaggaca aaattcgtcc tttctttttt
     3961 gatgttcaga gcgataaaaa tccttttttt gaagttttca aagttccgaa a
//
LOCUS       DAVDWT010000073         3740 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVDWT010000073 DAVDWT010000000
VERSION     DAVDWT010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.073"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            1..588
                     /locus_tag="VUW50_002076"
                     /pseudo
     CDS             1..588
                     /locus_tag="VUW50_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800706.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
     gene            complement(942..1475)
                     /locus_tag="VUW50_002077"
     CDS             complement(942..1475)
                     /locus_tag="VUW50_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HET7983895.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(1564..2340)
                     /gene="recX"
                     /locus_tag="VUW50_002078"
     CDS             complement(1564..2340)
                     /gene="recX"
                     /locus_tag="VUW50_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HET7983896.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDINENIVSQVIANLKEEKW
                     INDSQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDEETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2378..>3740
                     /gene="rlmD"
                     /locus_tag="VUW50_002079"
     CDS             2378..>3740
                     /gene="rlmD"
                     /locus_tag="VUW50_002079"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET7983897.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKEGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     IRKS"
BASE COUNT         1243 a          612 c          664 g         1221 t
ORIGIN      
        1 ggattggagg taataaaaaa tgagcaacaa aaactttttg agaagagtgg ggaaaaaact
       61 cataaaataa atacaattat ggttcaggga ttatgaaatc agctagattg taatcatgta
      121 gttagtagag atgatctaaa tttagtatat gactatttat tccagaagga acgatgggag
      181 tcctatgaga taacgctgat tggtaattta tatcatcttt ttgaaacaga ttatatttac
      241 atggtcggga aagaaatact agagcgtaca cattattacg aaaaaatcgg caagaataga
      301 aatttagttg tgtcagcttg tttaaatttt tggttctgtt gccttgaaaa ttcacatcta
      361 atatatgcag actactttga aatgaaatta aaaaaattat taaaggatga cacaaaagtt
      421 tttgaaaaat ctacattcaa atttgtagaa ggatataaaa tatacctgac agaatctaaa
      481 gaatctggaa ttaaacaaat ggacaatgtc ataaaatatt ttgagtttat tgaatctaaa
      541 agtattgctt tatattttca aaaacgatta aatgagctga tagattaaat agcattttct
      601 ctgttgagat attgttttta aaatattgta ctaaatgatt gatgctatgt ggaaatacaa
      661 aaaaatgttt ttgatacgaa gttgacctgt attttttata ctaatcattt tcgtattttt
      721 tgtattaaac gatataagtt tgttgtaaac ttacaaggaa taaagacatt aaaaaataac
      781 agtatatcta tttgttttat atattttacg aattctgcat aaatctcttt ctagtaatgt
      841 gttgtaactc tgctataata gatttattcc tttttgtgtt tacacaattt attttatact
      901 accaaaaaag gtcaggattt tgttcctgac ctttgacaac tttaccgatt ctttagttct
      961 acatagcgct tgtaccaaat gtttacatag gcttctgaga aaggaccacg tccattgtta
     1021 atccaatcaa caagaatttt gacatgttct tttaaaatat agtccaagtc atcagaataa
     1081 ttcattttgc gtttgtgacg ctcgtactct tcaacgtcca agagacgttt ttccccatct
     1141 gtaaaaattt taacatccaa atcgtaatca atatacttca gtgcttcttc atccagatag
     1201 taggggctag ccatattgca atagtaagaa gttccattat cacgaatcat ggcaatgata
     1261 ttaaaccaat atttcttgtg aaagtaaaca atagccggtt ctcgagtgac ccaacgacga
     1321 ccatcacttt cggtaacaag tgtatgatcg ttgacaccaa taatggcgtt ttctgttgtt
     1381 tttagtacca tggtgtctcg ccaagttcgg tggagactcc catcatgctt ataactttga
     1441 attgtaataa agtcgccttc ttttggaagc ttcataacta accaactttc tacaatttat
     1501 aagtttatca tttactattg taccataaaa ttacccaaaa tctgtgaatt tcacttggaa
     1561 atattaaaga tattctctaa gagcgcttgc tatatccgaa aaatcgtaac cctttcgtgc
     1621 taaaacttga gttaaacgct gcttcagttc gtatccttca tactttcggg catacttagt
     1681 atattgctta tcaagttcct tgaagatgag ttcctgagtc gtttcttcat caacttgact
     1741 atccaattcg tcaaaggcaa ttttagcatc aaaataagag aagcccttgt tagtcaagtt
     1801 ctggataatc ttatcttgca gggcacgagc tggaagtttt ccctcatatt ttttcaatag
     1861 tttattggct acacgttgag caacttccga aaaatcaaaa tcattcaaga tttcttctat
     1921 agtagatttt gaaattcctt tttgtgctaa tttctgagtc agtacataag gtcccttgtc
     1981 tcctgaaagt tgattggcat tgatgatagc ataagcgtac tggctatcat taatccactt
     2041 ctcttcttta agattagcaa tgacttgaga aacgatgttt tcattaatat catatttttt
     2101 cagatattct ctgacctctt tttcagtacg tgctttaaag gataagtggt agagggccag
     2161 attcttacca taagaaaatt gagcaaagtc ttgaatctct ttcaattcct cttcgcttat
     2221 caccttatct ctcgataaca taaaacgaac aattgtgtct tcggtgatat agcatttgtc
     2281 gccattatca agctccatca gatagagtct ttttttcttt tcaagttttg tgattttcat
     2341 agctctatta taactcaaaa tgtgataaga taggggtatg aatctgaaag tgaaacaaaa
     2401 aataccatta aaaatcaagc gcatgggaat taacggtgag ggaatcggct tttaccaaaa
     2461 aacattagtc tttgtaccag gagctctcaa aggcgaagat atctattgtc agattacttc
     2521 tattagacgc aactttgttg aagcaaaatt actgaaggtc aacaagaagt ctaaatttcg
     2581 aattgtgcca tcttgtacta tttataatga atgcggaggc tgccaaatca tgcacctgca
     2641 ttatgataag cagctggagt tcaagacgga cttacttcat caagcgctga aaaaatttgc
     2701 tcctgcagga tatgaaaatt atgaaattcg tccaactatt ggaatgcagg aaccaaaata
     2761 ttacagagct aagttacaat ttcagactcg aaaatttaaa aatcaggtca aggagggctt
     2821 atatgcacaa aactctcact atttagtaga gttgaaagac tgcctggtac aagataagga
     2881 aacccaagtg attgctaatc gcttagcaga attacttact tatcaccaga ttccaatcac
     2941 ggatgagaga aaagttctag gtgtccgtac tattatggtc cgacgcgcga gaaagaccgg
     3001 acaggttcag attattattg ttacaaaccg ccagcttaat ttaactcaat tggtaaaaga
     3061 gttggttaaa gatttcccag aagttgtgac agtagctgtt aatacaaata cagctaaaac
     3121 cagtgagata tatggtgaaa agacagagat tatctggggg caagagagta ttcaagaagg
     3181 tgtactcaat tatgaatttt cactatcccc tcgagctttt tatcaactaa atcctgagca
     3241 aacagaagtc ctctatagcg aagcagtaaa agcgctggat gttgataaag aagaccattt
     3301 gattgacgct tattgtggag ttggaacgat tggatttgcc tttgcaaaga aagtaaaaac
     3361 actcagaggt atggatatta ttccagaagc tattgaagat gccaagcgaa atgctaaaag
     3421 aatgggattt gacaatactc attatgaagc tggaacggca gaagagatta ttcctcgttg
     3481 gtacaaggaa ggctaccgag cagatgcttt gattgttgac ccaccacgta caggtctgga
     3541 tgataagtta ttagatacta ttcttactta tgtaccagaa aaaatggttt atatttcttg
     3601 taatgtttcg accttggctc gtgatttggt acgcttagta gaagtctatg atcttcatta
     3661 tatccagtcg gtcgatatgt tcccacatac agctcgaact gaagctgttg taaaattaat
     3721 aaaaaaaatt agaaaaagta
//
LOCUS       DAVDWT010000074         3461 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVDWT010000074 DAVDWT010000000
VERSION     DAVDWT010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.074"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            38..247
                     /locus_tag="VUW50_002080"
     CDS             38..247
                     /locus_tag="VUW50_002080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983898.1"
                     /translation="MKQKNYLVSNATVRQTYDKIAESECFLRAIGGIMLHLKLVKQEI
                     EAEKPASVEAWIISVKFKKGCYRHI"
     gene            248..610
                     /locus_tag="VUW50_002081"
     CDS             248..610
                     /locus_tag="VUW50_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983899.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHTFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(682..1531)
                     /locus_tag="VUW50_002082"
     CDS             complement(join(682..1116,1118..1531))
                     /locus_tag="VUW50_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HET7983900.1"
                     /translation="MKKTLTPTKESIDSLRNYEASKQLTDTRFKRLVGVQRTTFKEML
                     AVLKTAYQLKHAKGGRKPKLSLEDLLMATLQYVREYRTYEQIAAVFGIHESNFIRRSQ
                     WVEVTLVQSGVTISRTPLSSEDTVMIDATEVKINRPKKELANDSGKKKCHAMKAQAIV
                     TSQGRIVSLDITVNYCHDMKLFKMSRRNIGQAGKILADSGYQGPMKIYPQAQTPRKSS
                     KLKPLIAEDKAYNHALSKERSKVENIFAKVKTFKIFSTTYRNHRKRFGLRMNLIAGII
                     NHELGF"
     gene            complement(1476..1658)
                     /locus_tag="VUW50_002083"
     CDS             complement(1476..1658)
                     /locus_tag="VUW50_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7983901.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDPYQGVNRLPAKL"
     gene            1993..>3461
                     /gene="pcpA"
                     /locus_tag="VUW50_002084"
     CDS             1993..>3461
                     /gene="pcpA"
                     /locus_tag="VUW50_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HET7983902.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLWYYLNESGSMATGWVKDKG"
BASE COUNT         1178 a          534 c          612 g         1137 t
ORIGIN      
        1 aaaacaagaa caccttgctt ggcgcagggt gtttcgcgtg aaacagaaga attatctggt
       61 ttcaaatgct acagttagac aaacttatga taaaatagca gaaagtgaat gtttcctaag
      121 agcaattgga ggtattatgc tacacttaaa attagtaaaa caagaaatag aagctgaaaa
      181 gccagcatct gtagaagctt ggatcatttc cgtcaaattt aaaaaaggtt gctaccgaca
      241 tatatagatt ccaaaaacaa aaacgttagc ggaactagca gatgtgattt tatggagttt
      301 tgattttgca aatgatcatg ctcacacatt tttcatggat aatgttgagt ggagtcatgc
      361 agattcttac tttcttagct ttgttagtga cgatgttgaa gaacgttaca cagaaaatgt
      421 ctatctggat agcctaagtg tcaaacaaaa atttaagttt attttcgact tcggtgatga
      481 atggcgtttt gaatgccaag tgctgagaga aatcgagaca gaggacgaag aagcttatct
      541 cgtacgttcg gttggaacgt cgccagaaca atatccagat tatgatggtt ttgactatga
      601 agaatggtaa aattgaaatc taaaaaacaa ttggaatcag tctgtgtgta ggcttagtat
      661 ttcaatagac ttcctgcaaa actagaatcc tagttcatga ttgataatac cagcaatcaa
      721 attcattcgt aatccgaagc gtttacgatg atttcgatag gttgttgaaa atattttaaa
      781 cgtttttact ttggcaaaga tgttctcaac cttgcttctc tccttagata gcgcatggtt
      841 ataggcttta tcttcagcta ttagcggctt gagtttgctg gatttacgtg gagtttgtgc
      901 ttgaggatat atcttcatgg gcccttgata accactgtca gccaagattt taccagcttg
      961 tccgatattt ctgcgactca ttttgaacaa cttcatatca tgacaatagt tcacagtgat
     1021 atccaaagaa acaattctcc cttgacttgt gacaatcgct tgagccttca tagcgtggca
     1081 tttcttttta ccagaatcat tcgctaattc tttttttagg gcgattgatt tttacttccg
     1141 tcgcatcaat cattaccgtg tcctcagaac tgagaggagt tcttgaaatc gtaacaccac
     1201 tttgaacaag agttacttca acccattggc tccgacggat aaagttgctt tcgtggatac
     1261 caaaaacagc cgcaatttgt tcataagttc gatattctcg cacatattga agagtggcca
     1321 taagaaggtc ttctagactt aatttaggtt ttcgtccacc ttttgcgtgt ttaagttgat
     1381 aagctgtttt taatacagct aacatctctt taaaagtggt acgctgaaca ccaacaagac
     1441 gcttaaatcg tgtatcagtt aattgtttac ttgcttcata atttcgcagg gagtctattg
     1501 actccttggt aggggtcaat gtttttttca tctatcccga gaattatttt cccgccattt
     1561 gtatttgcaa atgctgagta ggtttcccag aaagactctg gaagattatt tttagctttt
     1621 ttgtattcta aatcaacccc ttcaaatttt aagtccatat ttttccttta catctgtttt
     1681 ttgtggttct ggtatttgtt caagttgagt gataatatag cgaattgaat ttcgagagtt
     1741 tttactcagt taatttcttt tttaacccgc tttaatttct tttttaacac gtgttaaaaa
     1801 agaaattaaa gcgggttaat ttttcttgac ttaaaattta aaaatgatat actgtgttta
     1861 tggagagatg acaatttata aaatgtacta ctctattcta ctagataatg attgaataaa
     1921 actttttata acaacggcta gcaaagttat aattttatat ctattagaaa ataaatatgt
     1981 aaggaaattt aaatgaaaaa aactacaata ttatcattaa ctacagctgc agttatttta
     2041 gcagcatatg tccctaatga accaatccta gcagatactc ctagttcgga agtaatcaaa
     2101 gagactaaag ttggaagtat tattcaacaa aataatatca aatataaggt tctaactgta
     2161 gaaggtaaca taggaactgt tcaagtgggt aatggagtta ctcctgtaga gtttgaagct
     2221 ggtcaagatg gaaaaccatt cacgattcct acaaaaatca cagtaggtga taaagtattt
     2281 accgttactg aagtagctag tcaagctttt agttattatc cagatgaaac aggtagaatt
     2341 gtctactatc ctagctctat tactatccca tcaagcataa aaaaaataca aaaaaaaggc
     2401 ttccatggaa gtaaagctaa aactattatt tttgacaaag gtagtcagct ggagaaaatt
     2461 gaagatagag cttttgattt ttctgaatta gaagagattg aattgcctgc atctctagaa
     2521 tatattggaa caagtgcatt ttcttttagt caaaaattga aaaagctaac cttttcctca
     2581 agttcaaaat tagaattaat atcacatgag gcttttgcta atttatcaaa tttagagaaa
     2641 ctaacattac caaaatcggt taaaacatta ggaagtaatc tatttagact cactactagc
     2701 ttaaaacatg ttgatgttga agaaggaaat gaatcgtttg cctcagttga tggtgttttg
     2761 ttttcaaaag ataaaaccca attaatttat tatccaagtc aaaaaaatga cgaaagttat
     2821 aaaacgccta aggagacaaa agaacttgca tcatattcgt ttaataaaaa ttcttacttg
     2881 aaaaaactcg aattgaatga aggtttagaa aaaatcggta cttttgcatt tgcagatgcg
     2941 attaaacttg aagaaattag cttaccaaat agtttagaaa ctattgaacg tttagccttt
     3001 tacggtaatt tagaattaaa agaacttata ttaccagata atgttaaaaa ttttggtaaa
     3061 cacgttatga acggtttacc aaaattaaaa agtttaacaa ttggtaataa tatcaactca
     3121 ttgccgtcct tcttcctaag tggcgtctta gattcattaa aggaaattca tattaagaat
     3181 aaaagtacag agttttctgt gaaaaaagat acatttgcaa ttcctgaaac tgttaagttc
     3241 tatgtaacat cagaacatat aaaagatgtt cttaaatcaa atttatctac tagtaatgat
     3301 atcattgttg aaaaagtaga taatataaaa caagaaactg atgtagctaa acctaaaaag
     3361 aattctaatc agggagtagt tggttgggtt aaagacaaag gtttatggta ttacttaaac
     3421 gaatcaggtt caatggctac tggttgggtt aaagacaaag g
//
LOCUS       DAVDWT010000075         3369 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVDWT010000075 DAVDWT010000000
VERSION     DAVDWT010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.075"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            170..823
                     /gene="rr06"
                     /locus_tag="VUW50_002085"
     CDS             170..823
                     /gene="rr06"
                     /locus_tag="VUW50_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HET7983903.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            820..2151
                     /gene="hk06"
                     /locus_tag="VUW50_002086"
     CDS             820..2151
                     /gene="hk06"
                     /locus_tag="VUW50_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HET7983904.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            2234..2761
                     /locus_tag="VUW50_002087"
     CDS             2234..2761
                     /locus_tag="VUW50_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HET7983905.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDSMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            2840..>3369
                     /locus_tag="VUW50_002088"
     CDS             2840..>3369
                     /locus_tag="VUW50_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HET7983906.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEEVGGRN
                     TPTVTSSGQDISKKYADEVESHLKKILSEIQTQLDRKRHTETVALINELQGIKKTYLY
                     NLNVLKEKSKVELPSKIKAKLDVAFDQFKKDTLKPGEKVAEAQKKVAEAKKKAEDQKE
                     EDRRNYPTNTYKTLEL"
BASE COUNT         1134 a          460 c          702 g         1073 t
ORIGIN      
        1 gagtataagc tgcaagatga atgattttct tgtattgacg ttgttgttga caaaaagtag
       61 cggataaatg aaatccattc cattatcata gatgataggc tggtaggaaa ttttcaaata
      121 gcatacagga aatagatgta tggagttctg gtagtagaaa gggagagaga tgaacatttt
      181 agttgcagat gacgaggaaa tgattagaga aggaattgca gcatttctga cagaagaggg
      241 ttatcatgtc attatggcta aggatggaca agaggtcttg gaaaaatttc aagatctccc
      301 tatccatctc atggtactgg atttaatgat gcctaggaag agtggttttg aagtgttaaa
      361 agaaatcaat caaaagcacg atattcctgt catcgtcttg agtgctctgg gagatgaaac
      421 tactcagtca caggtatttg atctctatgc tgatgatcat gtgacaaaac ctttttcttt
      481 ggtactgctt gtcaagcgta ttaaggcgct tatcagacgt tactacgtca tagaggatct
      541 ttggcgatat caggatgtaa cagtggattt tacctcttac aaagcacatt ataaaaatga
      601 agaaattgat ctcaaaccaa aggaattact ggtactaaag tgtttgattc agcataaaaa
      661 tcaagtttta agtagagagc agatattgga agaaatttca aaagatgtag ctgatttacc
      721 ttgtgatagg gtcgttgatg tctatattcg tactcttcgc aaaaaattag ctttagattg
      781 tatcgtgact gtgaaaaatg ttgggtataa gattagctta tgataaaaaa tcctaaatta
      841 ttaaccaagt cttttttaag aagttttgca attctaggtg gtgttggtct agtcattcat
      901 atagctattt atttgacctt tcctttttat tatattcaac tggaggggga aaagtttaat
      961 gagagcgcaa gagtgtttac ggagtattta aagactaaga catctgatga aattccaagc
     1021 ttactccagt cttattcaaa gtccttgacc atatctgctc accttaaaag agatattgta
     1081 gataagcggc tccctcttgt gcatgacttg gatattaaag atggaaagct atcaaattat
     1141 atcgtgatgt tagatatgtc tgttagtaca gcagatggta aacaggtaac cgtgcaattt
     1201 gttcacgggg tagatgtcta caaagaagca aagaatattt tgcttttgta tctcccatat
     1261 acatttttgg ttacaattgc tttttccttt gttttttctt atttttatac taaacgcttg
     1321 ctcaatcctc ttttttacat ttcagaagtg actagtaaaa tgcaagattt ggatgacaat
     1381 attcgttttg atgaaagtag gaaagatgaa gttggtgaag ttggaaaaca gattaatggt
     1441 atgtatgagc acttgttgaa ggttattcat gagttggaaa gtcgtaatga gcaaattgta
     1501 aaattgcaaa atcaaaaggt ttcctttgtc cgcggagcat cacatgagtt gaaaacccct
     1561 ttagccagtc ttagaattat cctagagaat atgcagcata atattggaga ttacaaagat
     1621 catccaaaat atattgcaaa gagtataaat aagattgacc agatgagcca cttattggaa
     1681 gaagtactgg agtcttctaa attccaagag tggacagagt gtcgtgagac cttgactgtt
     1741 aagccagttt tagtagatat tttatcacgt tatcaagaat tagctcattc aataggtgtt
     1801 acaattgaaa atcaattgac agatgctacc agggtcgtca tgagtcttag ggcattggat
     1861 aaggttttga caaacctgat tagtaatgca attaaatatt cagataaaaa tgggcgcgta
     1921 atcatatccg agcaagatgg ctatctctct atcaaaaata catgtgcgcc tctaagtgac
     1981 caagaactag aacatttatt tgatatattc tatcattctc aaatcgtgac agataaggat
     2041 gaaagttccg gtttgggtct ttacattgtg aataatattt tagaaagcta tcaaatggat
     2101 tatagttttc tcccttatga acacggtatg gaatttaaga ttagcttgta gacagattag
     2161 ttttttatta aagttcatat agggttaaca taagtgtgtt attctttgtg tagataaaag
     2221 aaaggatact aatatggtat tagcgattat tttagtaaca ttccttattc gattgatttt
     2281 tttaaagcgt tcgatagaga atgagaaacg aatccttagc aatggcggga aagaatttgg
     2341 agttgagaat acaaaacgat taactatggc tcatattgtt ttttatctct cttgcttggt
     2401 tgaggcaatg gtgcacaaga caatttttga tagcatgggc atggttggtt tagtcttgct
     2461 tattttttct atgctgatgt taatgttggt gattcacttg ttgggagata tttggacagt
     2521 gaagcttatg cttgtcaata atcacaaata tgtagatcat atcttgttta ggacagtaaa
     2581 acatcctaat tactttttaa atattcttcc tgagttgatt ggcttgacct tgttgagtca
     2641 tgcctatatg acttttgttt tagtttttcc agtttatgca gttattttgt atcgacgaat
     2701 agctgaagag gaaaagctat tacatgaagt tataatccca aatggaagca taaagagata
     2761 aatacaaaat tcgatttata tacagttcat attgaagtga tatagtaagg ttaaagaaaa
     2821 aatatagaag gaaataaaca tgtttgcatc aaaaagcgaa agaaaagtac attattcaat
     2881 tcgtaaattt agtattggag tagctagtgt agtagttgct agcttgttct taggaggagt
     2941 agttcatgca gaagaggttg gtggtaggaa tacccccacg gttacatcta gtgggcaaga
     3001 tatatcgaag aagtatgctg atgaagtcga gtcgcatcta aaaaaaatat tgagtgagat
     3061 ccaaacacaa ttagatagaa aacgacatac cgaaactgta gcgctaatta acgagttgca
     3121 aggcattaag aaaacgtatt tgtataattt aaatgtttta aaagagaagt cgaaagttga
     3181 gttgccgtca aaaataaaag caaagttaga cgtagctttt gatcagttta aaaaagatac
     3241 attgaaacca ggagaaaagg tagcagaagc tcagaagaaa gttgcagaag ctaagaaaaa
     3301 agccgaggat caaaaagaag aagatcgccg taactaccca accaatactt acaaaacgct
     3361 tgaacttga
//
LOCUS       DAVDWT010000076         3291 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVDWT010000076 DAVDWT010000000
VERSION     DAVDWT010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.076"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            438..1784
                     /gene="gdhA"
                     /locus_tag="VUW50_002089"
     CDS             438..1784
                     /gene="gdhA"
                     /locus_tag="VUW50_002089"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HET7983907.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGSVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSNLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
     gene            complement(2280..2450)
                     /locus_tag="VUW50_002090"
     CDS             complement(2280..2450)
                     /locus_tag="VUW50_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983908.1"
                     /translation="MKKENEYVILTIASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(2463..3041)
                     /locus_tag="VUW50_002091"
                     /pseudo
     CDS             complement(2463..3041)
                     /locus_tag="VUW50_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(3058..>3291)
                     /locus_tag="VUW50_002092"
     CDS             complement(3058..>3291)
                     /locus_tag="VUW50_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7983909.1"
                     /translation="KIYPQAQTPRKSSKLKPLTVEDKAYNHALSKEKSKVENIFAKVE
                     TFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
BASE COUNT         1013 a          602 c          563 g         1113 t
ORIGIN      
        1 actatagaac gatttgaagg cgtttatgat atttagctgt acgagagtct tttaaaagtg
       61 ttttgatggc ttggatttct tctttagttg atttcatatc actattatat aatgattttt
      121 gattttagtc tggtataaat attgctttcc tccaaaatgg tcataatttt actagcaaat
      181 ctaacatatc acggataaat taacaagtga tttctgaatt gctaaacatt ttcttttctt
      241 atagcatacc atactttaag attttgtctt tgagaaagat atttccaaga aaaacgttcg
      301 tttttttatc tattttcaaa aaaaccaaaa actgaattga aagaattttt agaaaatttc
      361 tgttttttct ttacagtgaa aaaaaattct gctataatgt cttatgtaat aaaatatgac
      421 aataaaagga gaacgatatg acatctgcta aagaatatat ccaaagcgtg tttgaaactg
      481 tgaaagctcg taacgggcac gaggctgaat tcctccaagc tgttgaagaa tttttcaaca
      541 ctttggaacc tgtatttgaa aaacaccctg agtatatcga agaaaatatc ttggcacgta
      601 ttactgagcc tgagcgcgtg gtttctttcc gtgttccttg ggttgaccgt gatggaaaaa
      661 ttcaagtaaa ccgtggttac cgtgttcaat tcaactcagc tgttggacca tacaaaggtg
      721 gacttcgttt ccacccaact gtaaaccaag ggatcttgaa attcctcgga tttgaacaaa
      781 tctttaaaaa tgtcttgact ggacttccta tcggtggagg taaaggtgga tcagacttcg
      841 atcctaaagg taaaacagat gctgaagtga tgcgcttctg ccaaagcttc atgactgaat
      901 tgcaaaaaca catcggacca tcacttgacg tacctgctgg tgatatcggt gttggtggac
      961 gtgaaattgg ttacctttac ggtcaataca aacgtcttaa ccaatttgat gctggtgtct
     1021 tgactggtaa acctcttgga tttggtggta gcttgattcg tccagaagca actggttacg
     1081 gtttggttta ctatactgaa gaaatgctca aagctaacgg taacagcttt gctggtaaga
     1141 aagtcgttat ttcaggttct ggtaacgttg ctcaatatgc tcttcaaaaa gcaactgaac
     1201 tcggtgcaac tgttatttct gtttctgact caaatggtta tgtcatcgat gaaaatggta
     1261 tcgacttcga tcttttggtt gatgttaaag aaaaacgtcg tgctcgtttg actgagtatg
     1321 cagctgagaa agcaactgct acttatcatg aaggttctgt atggacttac gctggaaact
     1381 atgacattgc gcttccatgt gctactcaaa acgaaatcaa cggtgaagca gctaaacgtt
     1441 tggttgctca aggcgttatc tgtgtatctg aaggtgccaa catgccaagc aaccttgatg
     1501 ccatcaaagt ttacaaagaa aatggtatct tctacggacc tgccaaagct gccaacgctg
     1561 gtggtgtagc cgtttcagcc cttgaaatga gccaaaacag ccttcgcctc tcatggactc
     1621 gtgaagaagt tgatggccgt ctcaaagaca tcatgacaaa catcttcaac acagctaaaa
     1681 cgacttcaga aacatacggt cttgataaag actaccttgc aggagctaac attgctgcct
     1741 ttgaaaatgt agcaaacgct atgattgccc aaggtattgt ttaagattta tttgcttaat
     1801 cctcaatcac tacgattggg gatttttagt tgagttagac agttgggaaa ttctaattaa
     1861 atgaaataaa aactgaacaa attgattgcg gaattcaaac caatttctaa catacttcta
     1921 gaaattaatt tgacttttct aatcgatttg ttcatatctt atttcaattt actatactta
     1981 cttaaccctt tttatactca atgaaaatca aagagcaaac taggaagcga gccgcaggct
     2041 gtacttgagt acggcaaggc aaagctgacg tggtttgaag agattttcaa agagtattaa
     2101 acaaaaagaa aaaccgctac tcctaagaat agcgatttaa tcatgttttt aagctactac
     2161 tgtagttgct ctattattta agtgttatga ataacttcaa tatctttaaa gctttttgtt
     2221 agatttataa tttcaactat agccatctct actccttagt ttacagcaaa aaattatgct
     2281 taattcttat tgtttttgta aacaatcagc gaacccaata ctattccatt caacaagcct
     2341 aaagaaatac catattcaac tggaaaatct aaaaaaattg caaacactat tccaatcatc
     2401 acccctagtg aggctattgt taaaattaca tattcatttt ctttcttcat tttctttaca
     2461 cctcatttcc tttttgtata taagctgtag tactctccac caagagtcat taagtactta
     2521 tgatttcctg actcaacaat cttaccacct ttcattacaa aaataatatc cgcatcctta
     2581 atcgttgaca atctatgagc tataattatt tgagtacaac cctgactttg tatatacttt
     2641 gttattcttt cctcattaat agtgtctaat gcactagttg cttcatctaa aattacaata
     2701 ctaggattat ttattaatgc acgtgccagt gctatccgtt gcctttgccc acctgaaata
     2761 tttgacccca tctctgagat gatagtatta aatttcatcg gcatagccat gatttcatca
     2821 tagatttgaa ctgctttaca aacttcctct atcttttgtg aagtaacttc gtgctttaaa
     2881 gttatattat caagaatact tctgttcaat aaaaacgaat cctgtggaac tactcctaaa
     2941 ttttgactta atattttttt atctatttga ttaatattta caccatcaaa taaaactttt
     3001 cctgtatcaa tcttatataa tcttgatggt atttttgata atagacttcc tgcaaaacta
     3061 gaatcctagt tcatgattga taatgccagc aatcaaattc attcgtaatc cgaagcgttt
     3121 acgatgattt cgataggttg ttgaaaacat tttaaacgtt tctactttgg caaagatgtt
     3181 ctcaaccttg cttttctcct tggatagcgc atggttataa gctttatctt caactgttag
     3241 cggcttgagt ttgctggatt tacgtggagt ttgtgcttga ggatatatct t
//
LOCUS       DAVDWT010000077         2535 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVDWT010000077 DAVDWT010000000
VERSION     DAVDWT010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.077"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..559
                     /locus_tag="VUW50_002093"
     CDS             <1..559
                     /locus_tag="VUW50_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET7983910.1"
                     /translation="EVPCEIPESWEWVRLNDITSYIQRGKSPKYSNISIYPVIAQKCN
                     QWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWA
                     VADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIP
                     LPPLPEQSRIVDKIEQFFAHIDALI"
     gene            616..1413
                     /gene="psrA"
                     /locus_tag="VUW50_002094"
     CDS             616..1413
                     /gene="psrA"
                     /locus_tag="VUW50_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HET7983911.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLTFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            1424..2029
                     /locus_tag="VUW50_002095"
     CDS             1424..2029
                     /locus_tag="VUW50_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET7983912.1"
                     /translation="MVKLTTCYFCDYGSYYGNIPMNWVVIKIKDIFSINTGLSYKKGD
                     LSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHI
                     GKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQA
                     LYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQLWK"
     gene            complement(1977..>2535)
                     /locus_tag="VUW50_002096"
     CDS             complement(1977..>2535)
                     /locus_tag="VUW50_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET7983913.1"
                     /translation="NKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEAIFWGT
                     EIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIISKVDLLFQKVSQLFE"
BASE COUNT          870 a          363 c          434 g          868 t
ORIGIN      
        1 ggaagtacct tgtgaaatac cagaaagttg ggaatgggtg aggttaaacg atataacctc
       61 ctacattcaa agagggaagt ctccaaaata ttcaaatatt tctatatacc ctgtgattgc
      121 tcagaaatgt aatcaatggt ctggcttttc tattgactta gcaagattta ttgatcccga
      181 aacagttcat agttaccaga aagaaagatt actaagagat ggtgatttaa tgtggaattc
      241 aacaggtcta ggtacgttag gacgattagc tatttaccat gaaaataaaa atccttatgg
      301 ctgggctgtt gcagatagcc atgttacagt tataagagtc ttatcgggag ttattaattg
      361 ccatttcatc tataattttc tttcctctcc tattgttcag tcagttattg aagaaaaagc
      421 gtcgggtagt acaaaacaaa aagaattatt aacgaaaact ataaaagaat atttaatccc
      481 cctcccaccc cttcctgaac aatccagaat cgttgataaa atcgaacaat ttttcgccca
      541 tattgacgca ctaatttagt ataatagttt tacgtgcggt ggaatttcta tcaaacataa
      601 gataggagtt ttacgttgaa agattttgaa ttacatttga agaaagctgg tttagctgaa
      661 aatactgtgc gttcgtacct ttatggtgtt cgatttttct tagagaatta cgaactcaaa
      721 atggaagatt tatttgaata caaaggatat ttattagaca attttaaacc taaaactgtc
      781 aatttgcgac tacaaggagt aaataaatat ctgactttta ttggtcatga tgatttgaaa
      841 ttaaaatttg tcaaagtaca gcaaaaaccg tttttagaag atgtcattag ccatgctgat
      901 tacctttttc ttaaacgtag tttgaaaaaa gatggtattt taaaatggca ttttgtcgtt
      961 tggtttttag gtgcaactgg ggctcgtgta agtgaactta ttaaactaaa agtagagcat
     1021 gtagaaatcg gttattttga catttactca aaaggtggta aaattcgtag actgtacatt
     1081 cctaaaaaat taagaaatag ttgtcttagt tggttagaat cagaaaatcg ccgaagtggt
     1141 tatttatttt tgaataaatt taacgaacca attacagcaa gaggagttgc tcaacagtta
     1201 aaaaattatg ctgataaata caaaatgaat cctaaagtaa tttaccctca ttcttttagg
     1261 catttatttg ctaagaattt tttagcgaag tataatgata ttgccttgct tgcagatttg
     1321 atgggacacg aaagtataga aactactcga atttatctaa ggaaaacagc tactgaacaa
     1381 caaaatattg tagataaaat tgttaattgg taaaaaataa caggtggtca aactgactac
     1441 ctgctatttt tgtgattatg gctcttatta tgggaatata cctatgaatt gggttgttat
     1501 aaaaataaaa gatatttttt caataaatac aggtctttct tacaagaagg gcgatttaag
     1561 cattaataat aaaggtgtta gaattatacg tggtggtaat attaagcctt tagaattttc
     1621 tctgttggat aatgattact acattgatac acaattcatc tcctctgagc aagtttattt
     1681 aaaacataat cagctaataa cacctgtatc aacctcttta gaacatattg gaaagtttgc
     1741 aagaatcgat aaagactatg atggtgttgt ggctggtgga tttattttcc aattaacacc
     1801 attcgaaagt tcagagatta tttcaaaatt tctattattt aacttgtcct ctccgttatt
     1861 ttataaacaa ttgaaagcaa taactaaact atcaggtcaa gctttatata atattcctaa
     1921 aactacacta agcgagctat taattccgtt agctcctttt gaggaacagg aacttattac
     1981 tcaaaaagtt gagaaacttt ttgaaaaagt aaatcaactt tggaaatgat tcttttcatc
     2041 tcttcatgat tagaaatagg gattaataat tcggagatac tggtactatt taatgttttc
     2101 cctttgatag catcttttga atcacctaaa gtagagataa gtggcaaaaa tatcattaag
     2161 taatctctga taatattttc tttattagca taggggaata tcgatataat ggcttcatta
     2221 tgagtggcag gaatatccaa tatggcaact tttccaatag ataatttaaa actcattaat
     2281 aaagttcctt taggtgaaat gtctattttc tttgatttta atgctaattt agaaatagat
     2341 tctctcgtat tagttacata accagatata ggcatatctg atatagatac ccaaggtatt
     2401 tcagttcccc aaaaaatagc ttcactgcgt ggaggagttt ttcctattct gaagttaact
     2461 aggctagcaa atttaatata tctccatgct tctgggattt catatatagg ataagaggtt
     2521 gtttcgtctt tgttc
//
LOCUS       DAVDWT010000078         2478 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVDWT010000078 DAVDWT010000000
VERSION     DAVDWT010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.078"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..177
                     /locus_tag="VUW50_002097"
     CDS             <1..177
                     /locus_tag="VUW50_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7983914.1"
                     /translation="KLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSC
                     NTFYEALLSCSCFN"
     gene            380..1239
                     /locus_tag="VUW50_002098"
                     /pseudo
     CDS             380..1239
                     /locus_tag="VUW50_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001857688.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            1416..2423
                     /gene="gap"
                     /locus_tag="VUW50_002099"
     CDS             1416..2423
                     /gene="gap"
                     /locus_tag="VUW50_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET7983915.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHNVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
BASE COUNT          760 a          439 c          523 g          756 t
ORIGIN      
        1 aagttagaac tcttgtgtga agagtttggg cataaacttt tacctcttcc tccctactca
       61 cctgagtaca atcctattga gaaaacatgg gctcatatca aaaagcacct caaaaaggta
      121 ttaccaagtt gcaatacctt ttatgaggct cttttgtctt gttcttgttt caattgacta
      181 tatatcacgg atacagttgg gaaagaagct attaaatatt tggagagtcc ttttgggatg
      241 aacatgtcaa atattactat agatgggcaa gaaaagatgg gcaagaaatg gatctgagtc
      301 aaacgatgct tcttatgctc aagtagtgag tctttatgag gatacaagta tctctgttag
      361 taataatgag acggacaaag tgttggctgg atccctctat actgatacaa atgaacaggg
      421 attaacgatt ccaagcagtt tactaaaaaa ttggaatgaa cagacaggaa aaaatttgac
      481 agctaatgat cttattggca aatcagtctc agccagcatt gtagaaagtg ctgccgaagc
      541 tagtaagatt gctcaatttc aaacgaagat tgtacgtgta atcaatgatg aagatgacat
      601 ggaggacagc aacagtttca tgctgtctta tcaaatggaa acgattttga aagaggctgg
      661 atttacgaaa gctgtatctt attttatctt ggaactcaaa gatccatcac agacaaaagt
      721 agtaacagaa gagttacaga aaaataagaa gtatactgtg ctttctcaac agagagttct
      781 tgatattgtg attaccttta ttcgtgttat tcagggatta ttgattgtgc tttcatcaca
      841 agctattgtg gtagcagcgg ttatgattgg tatcattatt tacatcaata tcatgcagcg
      901 ttccaaggaa ataggtgtca tgaaggcagt tggttatcag aatcgtgatg tcaaaggaat
      961 ttttatttac gaggctatct ggattgtagg catcgccttg ctgctggcat ttttggttgc
     1021 acaaggggtg ggaagtttgg cgaatgcgat tgtaagtcac ttttacccat ccatcactaa
     1081 agtttttgaa ttaaatcttt tatctgtttt aggaactcta gttttcgctc tattacttgg
     1141 ttatgtctca gcctacttct acttggttat gtctcagcct acttcccggc gcgtaagatt
     1201 agtaaaatgg atcctgtaga atcgttacgc tatgaataat aagggattcc ttgatttaca
     1261 gtcttaaaaa gaaactcaca cagaaaaaca atggccatac ttgcaattca actcaaatag
     1321 ttgtataata ggtttgttga aaggtgattt gtagtgattc aagttactct tttcacaata
     1381 aaaatttcat gattttcata aggaggaaat cactaatggt agttaaagtt ggtattaacg
     1441 gtttcggacg tatcggtcgt cttgctttcc gtcgtatcca aaacgtagaa ggtgttgaag
     1501 ttacacgcat caacgacctt acagatccag ttatgcttgc acacttgttg aaatacgaca
     1561 caactcaagg tcgtttcgac ggtactgttg aagttaaaga aggtggattt gaagttaacg
     1621 gtaaattcat caaagtttct gctgaacgtg atccagaaca aatcgactgg gctactgacg
     1681 gtgtagaaat cgttcttgaa gctactggtt tctttgctaa gaaagaagca gctgaaaaac
     1741 accttaaagg tggagctaaa aaagttgtta tcactgctcc tggtggaaac gacgttaaaa
     1801 cagttgtatt caacactaac cacaacgttc ttgacggtac tgaaacagtt atctcaggtg
     1861 cttcatgtac tacaaactgc ttggctccaa tggctaaagc tcttcaagac aactttggtg
     1921 ttgttgaagg attgatgact actatccacg cttacactgg tgaccaaatg atccttgacg
     1981 gaccacaccg tggtggtgac cttcgccgtg ctcgcgctgg tgctgcaaac atcgttccta
     2041 actcaactgg tgctgcaaaa gctatcggtc ttgtaattcc agaattgaat ggtaaacttg
     2101 acggatctgc acaacgcgtt ccaactccaa ctggatcagt tactgaattg gtagcagttc
     2161 ttgaaaagaa cgttactgtt gatgaagtga acgcagctat gaaagcagct tcaaacgaat
     2221 catacggtta cacagaagat ccaatcgtat cttcagatat cgtaggtatg tcttacggtt
     2281 cattgtttga cgcaactcaa actaaagttc ttgacgttga cggtaaacaa ttggttaaag
     2341 ttgtatcatg gtacgacaac gaaatgtcat acactgcaca acttgttcgt actcttgaat
     2401 acttcgcaaa gattgctaaa taattcttga gttgatagaa agcaaggctt tgtggtcttg
     2461 ctttttatat ggaaaaat
//
LOCUS       DAVDWT010000079         2439 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVDWT010000079 DAVDWT010000000
VERSION     DAVDWT010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.079"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(151..1626)
                     /locus_tag="VUW50_002100"
     CDS             complement(151..1626)
                     /locus_tag="VUW50_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7983916.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTDKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSILLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            2333..>2439
                     /locus_tag="VUW50_002101"
     CDS             2333..>2439
                     /locus_tag="VUW50_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7983917.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASL"
BASE COUNT          742 a          531 c          447 g          719 t
ORIGIN      
        1 gctaccctaa tcctcagaaa aggcttgagg caggatttga taagctgata gaaattaagc
       61 gattgaccgc ttctaattca agggcttttt gccgtgctct aaaatcatcg ccaaatcatc
      121 aaattgaaat aagaaatagt ttagataaaa ctattgactt ttcttatagt ttgtctttct
      181 cttaataacg ttgatatgat tgacctctgg atcttccgtc ccttggaggg agcaaccacg
      241 ttccttggcg aattggatat agtcgacaat ttctcgtgta atgcgttcac caggagccaa
      301 gataggaata cctggagggt aacacataac aaattctcca cagacctgtc caacagaatc
      361 atccaaagtt aaactttttc tttctgaata gaaggcttct tgcggagaca gcactaactc
      421 gggctgaata tattctcctg ctatcaaatc ttttccatct cttgaataga gtctcttaat
      481 atcagccaga gcaccaacca agcgctcgat gtcttggatg cggtcgccga tggaaatata
      541 ggccaagata ttgccgatat caccaaactc gatctgaatg tcgtattcgt ctcgcaagag
      601 gtcataaacc tcgatacctg ttaagccaat accctgagtg taaactgaca gcttagttac
      661 gtcaaaatcg caaaccgaaa caccgtctat taactctttt gagtaggcat agtagccacc
      721 gatagcattg atttcacggc gggcatactc agatagctca atgactttct caaacgactc
      781 tttaccacga agggccaagt tgcgacgtga aatatccaaa ctagccatca acaagtaaga
      841 ggcagatgta gactgggtca ggttaattat ctgacgaacg tattcagaat tcatctgctc
      901 cccgataagt aaaatggagc tttgggtcaa actcccacca gacttatgca tggaaactgc
      961 tgccatatca gcccctgcat ccatagcaga aattggaagt ttatcagtga aatgcaaatg
     1021 cgctccgtgg gcttcatcta ctaaaaccat catgccagct tcatgagcca tttctgtcaa
     1081 cccctttagg tctgaacaga tgccgtagta agtaggattg ttgattagga tagccttagc
     1141 atctggatgg tcctttatgg cctgtgctac tcggtcattt tcaagaccta aagcgatacc
     1201 aatcttagga tctacactca tctcgatata gatgggaatg gcaccacata gaaccaacgc
     1261 attgatagca gatttatgga catttcgtgg cagaataatc ttatctcctg ccttgcaggt
     1321 tgccagaatc atagtctgca ccgatgaagt tgttccacca atcattagaa aggcatggct
     1381 agctccaaaa gcatctgcag ccagctcctc tgcatcacga ataatcgaaa taggatggcc
     1441 taaattatcc aaaggtttca tcgaattgac atcaatgcct acacattttt ctcctaagag
     1501 ttcgacaagt tctggatttc cccgtccacg cttgtgacct ggaacatcaa agggaacaat
     1561 ccttttcttg cgtaacttca ccaaggcctc ataaattggg gcttggtttt gatctaactc
     1621 tttcaaaaca tactcctttc aatattttta ggaccttatg aaaactaagg aactaaaggt
     1681 tgactcatga aaaaagagca ggttttcacc tgctctgcaa tcttctgacg tgtttatttt
     1741 ggtttctgtt gagtatgtct gttcccgatg tttcctaact ctctagtagc aaatattacg
     1801 tactttattg atcgtttcac aagatgacgt gtgctttcac cacactaaaa atttatgaat
     1861 taggacaagt gtcctaacat caacttataa aagtaggctt ttaaccctat caataatgtg
     1921 gcttttcaac cacgcttcgg cattcaaccc tgcctgtccg taggcatttt ttactcgggt
     1981 ttatatttgc tagaaaacat catctcattt tctaagcttt attactatat catgttttta
     2041 agaacttgta aagtattttt tgataaaaaa caaaaatata atagattgaa actagaatag
     2101 tacacctctg cttctaaaac attgttagaa atagatttta ctgtcctgat cgatttgttc
     2161 tgttcttgtt tcattttact atatgttcgt aaatgaaagt tctataatga agttagccac
     2221 ccagccctcc taaaaattag atggttcaac tagccgatag ctcttgtagg cataagaaaa
     2281 cacctctgtg ttatacttgt tgttcaacca caaacacaag aaaggcacag agatgcaaga
     2341 caattatact acaaaagcca aacatttgac aatcgatagc cgtcgcttaa tcgaaagatg
     2401 gaaaaaagaa gggaaatcaa atagagaaat tgcctctct
//
LOCUS       DAVDWT010000080         2178 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVDWT010000080 DAVDWT010000000
VERSION     DAVDWT010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.080"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(85..2082)
                     /gene="metG"
                     /locus_tag="VUW50_002102"
     CDS             complement(85..2082)
                     /gene="metG"
                     /locus_tag="VUW50_002102"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET7983918.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIIAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
BASE COUNT          622 a          476 c          426 g          654 t
ORIGIN      
        1 gcggctagct tcctagtttg ctctttgatt ttcattgagt ataaataaaa aaacagaact
       61 agcctgaact agtcctgtct acttttaccc aatcacactt ccatttggta cagctggatc
      121 aactgtgaga agggttaatt tgccatcatg ttcagctgag agaatcatac cctggctgac
      181 atattttttc atcattttac gtggtttgag gttagcaatg atttgaactt tcttgccgac
      241 caattcttgt tcatttggat agtattttgc aattcctgaa agaatctgac gatcttctcc
      301 atcaccagca tccaagcgga attgaagcaa cttatctgaa ccttctactt tagacacttc
      361 tttgacttct gccacacgga tttcaacctt gtcaaagtct tcaaacttga tttcatcctt
      421 gtttagtttg agctcaactt cgtccggatt ccattctttt tcgactgctg gtttattgcc
      481 ttccatttgt tccttgatat aggcgatttc ttcttccata tttagacgtg gaaagatagg
      541 tgttcctttg gcaactacag tcacatctgc tgggaagtca gccaaactca agttttcaag
      601 actagaaact tcttccaaac caagttgagt caaaactgca cgactagttt ccatcataaa
      661 tggttcaatc aagtgagcaa ctacacgaat gctggctgcc aagtggctca tgacacttgc
      721 caattggtca cgaagagctt catccttggc caagacccat ggtgcggtct catcgatgta
      781 tttattggta cgagagatca gagtccagac tgcttcaagc gcacgtggat agtcaactgc
      841 ttccatgtgt gtatggaagt ctgcgattga ttgttctgca acctcagcaa gaacatgatc
      901 aaattcagtc acaccttcta cataggcagg gatttgtcca tcaaagtact tattaatcat
      961 ggaaaccgta cggttaagga ggttcccaag gtcattagcc aattcatagt tgatacggcc
     1021 gacatagtct tcaggagtaa aggttccgtc tgaaccaact ggaaggttac gcatgaggta
     1081 gtaacgaagt ggatctagtc cataacgctc taccaacatt tcagggtaaa cgacattccc
     1141 ttttgactta gacatttttc cgtctttcat gacaaaccaa ccatgggcaa tcaaacgatc
     1201 aggtaattta acatccaaca tcataagaag gattggccag tagatagagt ggaagcgaag
     1261 gatatctttt cctaccatat ggaagactgt tccattccag aacttgtcaa agttaccatg
     1321 ttcgtcttga gcgtagccaa gagctgtcgc atagttaaga agggcatcaa tccaaacgta
     1381 gacaacgtgt tttggatttg atgggacagg cactccccat gtaaaggttg tacgagatac
     1441 cgccaaatct tccaagcctg gctcgatgaa gttgcgtagc atttcattaa ggcgaccatc
     1501 tggcgtgata aattcaggat gagctttgaa aaattcgacc aaacggtctt ggtatttgct
     1561 aaggcgaagg aagtatgatt cttcagaaac ccattcaacc tcatgacctg atggagcaat
     1621 accaccagtc acatttccag cttcatcacg gaaaacttct gccagctggc tttctgtaaa
     1681 gaattcttcg tctgatactg aataccaacc agagtattca cccaagtaga tatcatcttg
     1741 agcaagtaag cgttcaaaga cttgtgcgac aactttttca tggtagtcat cagttgtacg
     1801 gataaattta tcgtatgaga tatctagtaa ttgccagagt tctttaactc caaccgccat
     1861 tccatcaaca taggcttgag gtgtaatacc agcttcttcc gctttctgct ggattttctg
     1921 accatgttca tcaagacctg tcagataaaa tacatcgtag cccatcaggc gtttgtaacg
     1981 tgctaggaca tcacatgcga tagttgtgta ggcagaaccg atatgaagtt tcccagatgg
     2041 atagtaaatc ggcgttgtaa tataaaaatt tttttcagac ataatttttc ctttccaggc
     2101 aaatgaaacc tgtttttcta acacttcatt atatcacatt tttaatgaat ttcgataggg
     2161 aaatccatac aaaaacaa
//
LOCUS       DAVDWT010000081         2116 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVDWT010000081 DAVDWT010000000
VERSION     DAVDWT010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.081"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            65..604
                     /locus_tag="VUW50_002103"
     CDS             65..604
                     /locus_tag="VUW50_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7983919.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQEFESKGQEVDVFVTNFVYEKEGQSCKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTDLLRASQF"
     gene            671..1081
                     /locus_tag="VUW50_002104"
     CDS             671..1081
                     /locus_tag="VUW50_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HET7983920.1"
                     /translation="MHYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            1197..>2116
                     /gene="pavB"
                     /locus_tag="VUW50_002105"
     CDS             1197..>2116
                     /gene="pavB"
                     /locus_tag="VUW50_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HET7983921.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKEAVDTDDVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVK"
BASE COUNT          648 a          403 c          468 g          597 t
ORIGIN      
        1 tgacgcggtt tgaagagatt ttcgaagagt ataagaaaaa atcagtcccc taaaggacca
       61 gattatgaag ttattgtcta tcgccatttc tagctataat gcagcagcct atcttcatta
      121 ctgtgtggag tcgctagtga ttggtggtga gcaagttggg attttgatta tcaatgacgg
      181 gtctcaggat cagactcagg aaatcgctga gtgtttagct agcaagtatc ctaatatcgt
      241 tagagccatc tatcaggaaa ataaatgcca tggcggtgcg gtcaatcgtg gcttggcaga
      301 ggcttctggg cgctatttta aagtagttga cagtgatgac tgggtggatc ctcgtgccta
      361 cttgaaaatt cttgaaacct tgcaggaatt tgagagcaag ggtcaagagg tggatgtctt
      421 tgtgaccaat tttgtctatg aaaaggaagg tcagtcttgt aagaagagta tgagttacga
      481 ttcagtcttg cctgttcggc agatttttgg ctgggaccag gtcggaaatt tctccaaagg
      541 ccagtatacc atgatgcact cgctgattta tcggacagat ttgttgcgtg ctagccagtt
      601 ctaattgcct gaacatactt tttatatcga taatctcttt gtctttacgc cccttcagca
      661 ggtcaagacc atgcactatc tgcctgtcga tttctatcgt tatttgattg ggcgtgagga
      721 ccagtctgtc aatgagcaag tgatgattaa gtgcattgac cagcaactca aggtcaatcg
      781 actcttggta gaccagcttg atttgtccca agtgagtcat cccaaaatgc gagaatatct
      841 gctgaatcat attgaaatca cgacggtgat ttccagtacc ctgctcaacc gatctgaaac
      901 agcggagcat ctggcaaaaa aacgccaatt gtggacctat attcagcaga aaaatccaga
      961 agtctttcag gctattcgta agaccatgtt gagccgtttg accaaacatt ctgtcttgcc
     1021 agatcgcaaa ctgtccaatg tcgtctatca aatcaccaaa tctgtttatg gatttaatta
     1081 atataagtgt tttataagag ggatttaaga aaaattttaa ctttttctta gtccttttta
     1141 atttcaggag attatactag agtcatcaaa taaagaaaga ctctaaggag aatcctatga
     1201 aattcaatcc aaatcaaaga tatactcgtt ggtctattcg ccgtctcagt gtcggtgttg
     1261 cctcagttgt tgtggctagt ggcttctttg tcctagttgg tcagccaagt tctgtacgtg
     1321 ccgatgtggt caatccgacc ccaggtcaag tcttacctga agagacatcg ggaacgaaag
     1381 agggtgactt atcagaaaaa ccaggagaca ccgttctcac tcaagcgaaa cctgagggcg
     1441 ttactggaaa tacgaattca cttccgacac ctacagaaag aactgaagtg agcgaggaaa
     1501 caaactcttc tagtctggat acactttttg aaaaagatga agaagctcaa aaaaatccag
     1561 agctaacaga tgtcttaaaa gaagctgtag atacagatga tgtggatggg acacaagcaa
     1621 gcccagcaga aactactcct gaacaagtaa aaggtggagt gaaagaaaat acaaaagaca
     1681 gcatcgatgt tcctgctgct tatcttgaaa aagctgaagg gaaaggtcct ttcactgccg
     1741 gtgtaaacca agtaattcct tatgaactat tcgctggtga tggtatgtta actcgtctat
     1801 tactaaaagc ttcggataat gctccttggt ctgacaatgg tactgctaaa aatcctgctt
     1861 tacctcctct tgaaggatta acaaaaggga aatacttcta tgaagtagac ttaaatggca
     1921 atactgttgg taaacaaggt caagctttaa ttgatcaact tcgcgctaat ggtactcaaa
     1981 cttataaagc tactgttaaa gtttacggaa ataaagacgg taaagctgac ttgactaatc
     2041 tagttgctac taaaaatgta gacatcaaca tcaatggatt agttgctaaa gaaactgtcg
     2101 aaaaagctgt taaaga
//
LOCUS       DAVDWT010000082         1948 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVDWT010000082 DAVDWT010000000
VERSION     DAVDWT010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.082"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(<1..159)
                     /locus_tag="VUW50_002106"
     CDS             complement(<1..159)
                     /locus_tag="VUW50_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7983922.1"
                     /translation="MEQLHFITKLLDIKDPNIQILDIINKDTHKEIIAKLDYEAPSCP
                     ECGSQMKKY"
     gene            362..>1948
                     /gene="pspC"
                     /locus_tag="VUW50_002107"
     CDS             362..>1948
                     /gene="pspC"
                     /locus_tag="VUW50_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     LPXTG-anchored form"
                     /protein_id="HET7983923.1"
                     /translation="MFKSNHERRMRYSIRKFSVGVASVAVASLFMGSVVHATEKEGST
                     QAATSSKMANESQIERRKAAEQAIVSLSGYMTSLLNDGLDTSDSRFEELLKRAKGIVE
                     EYRKKIDGASDREKIEKLQQEGQTKLDELVDKFKKGLSSSEQNGHATPKDPSRNDGVQ
                     GDDSSVGQGEQPGASNQKNPESVPPTASGETDSPTKPKTSNDSVNSLERELKEVKETA
                     KTTLNTYMLTRLKQENPGVFWFADLLRESKESVEKYKRMFDEASSKENVENLVKEAKQ
                     EIEALVVKHKGREIDLERTKAKTVIAKYLTGLLDDIKKNLKKEQHINTVELIKKLGDI
                     KRTYLYKLDESTQKAQLQELVTESQSKLDEAFSKFKNGLSSSSSSGSSTKPETPQPEK
                     PEHQKPTTPAPDTKPSPQPEGKKPSVPDINQEKEKAKLAVATYMSKILDDIQKHHLQK
                     EKHRQIVALIKELDELKKQALSEIDNVNTKVEIENTVHKIFADMDAVVTKFKKGLTQD
                     TPKEPGNKKPSAPKPGMQPSP"
BASE COUNT          723 a          331 c          388 g          506 t
ORIGIN      
        1 atatttcttc atttgacttc cgcactcagg acaagatgga gcttcatagt ccagtttggc
       61 gatgatttcc ttgtgtgtat ccttattgat gatgtctaaa atctggatat tagggtcttt
      121 aatgtctagt aattttgtga taaaatgtaa ttgttccata tgattctttc taatgagttg
      181 ttttgtcgct tttcattata ggtcatatgg gacttttttt ctacacaaaa taggctccat
      241 aatatccata ggggatttac ccactacaaa tattatagag ccaaataatg tagcaaattt
      301 catatacaat tcatattagc aggatatact gtagttaatt aaaattatgg agaatataga
      361 catgtttaaa tcaaatcatg agcgaagaat gcgttattcc attcgtaaat ttagtgtagg
      421 agtagctagc gtagctgttg ccagtctttt tatgggaagt gttgtacatg cgacagagaa
      481 agagggaagt acccaagcag ccacttcttc taagatggca aatgaaagtc agatagaacg
      541 taggaaagct gctgaacaag ctatcgttag tttgtctggt tatatgacta gtcttctaaa
      601 tgatggatta gatacttctg attctagatt tgaagagctt ctcaagcgag ctaagggaat
      661 tgtagaagag taccggaaga aaattgatgg agcttctgat agagaaaaaa tagagaaact
      721 gcagcaagag ggacaaacga agctagatga attggtagac aaatttaaaa aagggttgtc
      781 cagctctgag caaaatggtc atgctactcc taaggatccc tctcgaaatg atggtgtaca
      841 gggagatgat tcttcagtag ggcaaggaga acagccaggc gcatctaatc aaaaaaatcc
      901 agaatctgta ccaccaacag catctggtga gacggattcc cctacaaaac caaagacttc
      961 gaatgattca gtgaattctc tggagagaga gcttaaagaa gttaaagaaa ctgctaaaac
     1021 aactttgaac acttatatgc ttactcgttt aaaacaggag aatccaggag ttttttggtt
     1081 tgcagatctt ttaagagaat ccaaagaatc tgtagagaag tataaaagga tgtttgatga
     1141 ggcttcgagt aaagaaaacg tggaaaactt ggtaaaagaa gctaaacagg agatagaagc
     1201 tctagtcgtt aaacataagg ggcgagaaat tgacttagaa cgaacgaaag caaaaaccgt
     1261 aattgctaaa tatttaacag gattattaga tgacatcaag aaaaatttaa aaaaagagca
     1321 acatatcaat actgtagagc taattaaaaa gctgggagac attaagcgaa cgtatttgta
     1381 taaattagat gaatcaaccc aaaaagccca actacaggaa ctggtcacag aaagtcaatc
     1441 aaaactagat gaagcttttt ctaaatttaa aaatggctta tcttcttcgt caagttcagg
     1501 atcctccact aaaccagaaa ctccgcagcc ggaaaaacca gagcatcaaa aaccaacaac
     1561 tccagctccg gataccaaac caagccctca accagaaggc aagaaaccaa gcgtaccaga
     1621 cattaatcag gaaaaagaaa aagctaagct tgctgtagca acctacatga gcaagatttt
     1681 agatgatata caaaaacatc atctgcagaa agaaaaacat cgtcagattg ttgctcttat
     1741 taaggagctt gatgagctta aaaagcaagc tctttctgaa attgataatg taaataccaa
     1801 agtagaaatt gaaaatacag tccacaagat atttgcagac atggatgcag ttgtgactaa
     1861 attcaaaaaa ggcttaactc aggacacacc aaaagaacca ggtaacaaaa aaccatctgc
     1921 tccaaaacca ggtatgcaac caagtcct
//
LOCUS       DAVDWT010000083         1860 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVDWT010000083 DAVDWT010000000
VERSION     DAVDWT010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.083"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(3..968)
                     /locus_tag="VUW50_002108"
     CDS             complement(3..968)
                     /locus_tag="VUW50_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET7983924.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            complement(1086..1499)
                     /locus_tag="VUW50_002109"
     CDS             complement(1086..1499)
                     /locus_tag="VUW50_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HET7983925.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            complement(1496..1732)
                     /locus_tag="VUW50_002110"
     CDS             complement(1496..1732)
                     /locus_tag="VUW50_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HET7983926.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
BASE COUNT          542 a          363 c          275 g          680 t
ORIGIN      
        1 ttttaagaga tgaatttttg gtgtgaatgc ttgacattat tttgattgac catggcatag
       61 gccaaggttg tgtcaatctt gctatgacct agcagttttt ggacttgttc gataggcata
      121 cctttatcaa ttgccttagt cgctaaagtt cttctgaact tatgtgggtg aaccttttgt
      181 atgccgagtt tgtctcctaa ctttcttaag cgaatctcta caccagcaat tccaagtctc
      241 tgggcttttc caactagcgt tacaaaaaga gcagggtgac tatcttttct gtcgttaaga
      301 taatttctta aatgaatttt cgtccgagcg tcaaaatata ctggtctctc cttctttcct
      361 ttaccaaaga caacacactc tctgttttca aaatcaatat ctgaacgatt caactgtaca
      421 agctccccta cacgcatacc tgtcgatgct agtaggtcta ttattgccaa atctctcaaa
      481 ttttcacagt tatcacgcat aatttccaaa tgttcatcag tataagtttc cttcacattt
      541 tgctcagtct taattttctt tatccgtcga atgggatttt taatgatata ttcctcttgc
      601 tcaagccaag caaaaaaaga agacaatata cgcctaatat tgtctaaatt tgccttacta
      661 caatcctttt cagactggta atttgctaaa taactcctta aatcatctgt tgtgagttgt
      721 gtcacagact ctttaatagc attaaacaag ttttcaatcg tcctctggta ataacgaatt
      781 gtttttgagc tacagccctc tacctgcttg gcggtaataa atagttgcaa caggttatta
      841 ttcgaatact tctgctgctt ttttccgtct actagatgat gttctaaact ctctctcaac
      901 gctagtaatt gttcattatt taaaagaggt aacatttctt gcatgataat tgtaactttt
      961 gattccataa tttctcactt tcttattcat caataaaaag agacaatatc agtttctgca
     1021 ttacagaatt ctaatattgt ctgttcattt attttcttgt gctcattcaa atccagcata
     1081 ctaggttact ttttgacctt ttctctaaca gaatgttcag aaacccattt ggagtagctt
     1141 gtcatttttt catcagttaa agcttctact gcgatggtta cacacatttt tactgcttct
     1201 tctaccgtta cagaaaaacg atagccgttt aattgtaaaa atttgaccaa aacgaagaaa
     1261 gcagttcttt tattagcatt agcaaaaaca tgcttcttta tcaattggac aaatagtatc
     1321 gttgctttat caaaaattgt tggataaaga gacttcccaa agacaaattg ttctggtaag
     1381 ttcacaatca tatttaaggc agaagaacta actgtttgaa ttttctcatt tggagaatac
     1441 cgttgaattg ctaaagcatt tattttttca atttgctttt ctgtcagata gattgtcatt
     1501 tttccaccaa agctttgaaa acatcgtcgt attcgtcaaa tattttatct aaatttaagt
     1561 caaaggcatc atcagataca taagaaacct gctgccccaa ttcttcagga gtaaagataa
     1621 tctcaccact tggtaacaat ttcgcttcgt atttaacacc acttggaata ttaaattcac
     1681 ttggaatcgt aatggtgatt gaatttcctt gttttcttgt ttttactacc atctgatttc
     1741 ctcctttgaa gtattataac aaatcattac cgtaattaca atggcagaat atcagccaaa
     1801 atactcctgc atcagagatt tcttcaaagt ttcaagttct tccagagatt tttggattgc
//
LOCUS       DAVDWT010000084         1713 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVDWT010000084 DAVDWT010000000
VERSION     DAVDWT010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.084"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            353..1333
                     /locus_tag="VUW50_002111"
     CDS             353..1333
                     /locus_tag="VUW50_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HET7983927.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFRIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAVEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAVYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
BASE COUNT          516 a          318 c          369 g          510 t
ORIGIN      
        1 aacatatata cggtaaggcg acgctgacgt ggtttgaaga gattttcgaa gagtattaag
       61 ctagttttta ggtttggctc ttatactaga gtcatcaaaa agaaacgagg actctcatat
      121 gacagtaacg attaaagtaa attaccaaac cactttccaa aagaaggaag caaaaaacta
      181 gtataaacag aagagagagc gaaatgctct ttttcgtttc taaaactact ttcagcccat
      241 catcctaaaa gtaaagaatc taaattcact ttctatttat ccttctttct tgcattgatt
      301 acatagatat gctacagttg tggtaacgat tacaaaataa aaggagcatg ctatgaaaaa
      361 tccagctttg ctagaagaaa ttaagaccta tagaggaagg gatgaggttc cggaagactt
      421 tgatgctttc tgggatgggg aagtgaaaaa tgtttccacg cttccatcct accacttgga
      481 ggaaagagat ttccgcattc ctcaagtcaa gtgctatgag ttaacatttg aaggaagcaa
      541 ggaaggaaag gtctatgcac gcattgttct tccaaagagt gaggagaagg ttccactaat
      601 cttccatttt catggttata tgggacgtgg ctgggactgg gccgacatgc tgggcttcac
      661 cgtagctggt tacggtgttg tttccatgga tgtgcggggc cagtcaggtt actcacaaga
      721 cggcttgcgt tctcctttag gaaataccgt gaaggggcat attatccgtg gtgctgtgga
      781 aggtcgggac cacctctttt ataaggatgt ttatctggat atttaccagt tggtcgaaat
      841 tgttgctagt ctgtctcagg ttgatgagaa gcgtctttct agctatggtg cctcacaagg
      901 aggggctcta gccctagttg cagcagcgct caatcctcga attcagaaaa cagttgctgt
      961 ttatcccttc ttgtcagact tcagacgggt gattgagatt ggtaatacta gcgaggctta
     1021 cgacgaactt ttccgttatt tcaagtttca cgaccccttc catgaaacag aggaggaaat
     1081 catggcgacc cttgcctata tcgatgtcaa aaatcttgcc catcgtatcc aaggtgaggt
     1141 taagatgatt acgggcttgg acgacgatgt ttgctatccc attacccagt ttgcgattta
     1201 taatcgtctg acctgcgata aaacctatcg catcatgcct gagtatgctc acgaagccat
     1261 gaatgtattt gtcaatgacc aagtctacaa ctggctctgt ggaagtgaga ttccttttaa
     1321 atatctaaaa taaggagtcg actctaagca caaaatctta aaaattacaa acacgcatag
     1381 tatcagggga ttaaaaaaac ttgatactat gcgttttatc atggaaatat agtaaaatga
     1441 aacaagaata ggacaaaacg atcaggacag tcaaatcgat ttctaacaat gttttagaag
     1501 caaatgtgta ctattctagt ttcaatctat tatatttata gaattttttg ttgctagatt
     1561 tgtcaaattg cttaaaataa tttttttcag aaagcaaaag ccgataccta tcgagtaggg
     1621 tagttcttgc tatcgtcagg cttgtctgta ggtgttaata cttttcaaaa atctctttaa
     1681 accacgtcag cttcgccttg ccgtatatat gtt
//
LOCUS       DAVDWT010000085         1191 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVDWT010000085 DAVDWT010000000
VERSION     DAVDWT010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.085"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            151..558
                     /locus_tag="VUW50_002112"
     CDS             151..558
                     /locus_tag="VUW50_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7983928.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKIG"
     gene            723..1022
                     /locus_tag="VUW50_002113"
     CDS             723..1022
                     /locus_tag="VUW50_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HET7983929.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
BASE COUNT          421 a          234 c          201 g          335 t
ORIGIN      
        1 tacgaaaatc tattgaatat gccataagaa gattatacct cattgtgtac tatttttggt
       61 tcattttact ataataataa cgatactaga taatgaggaa aatgcaatgg caaacccaac
      121 tttcggagaa aaaaagcgaa tacagactat attgctcgct atggcgtata tgcagttatc
      181 cctaatcctg aacaaaaaca aattgttctt gttcaagaac caaatggtgc ttggttccta
      241 ccatgtggag aaattgaagc aggtgaaaat catcaggaag ccctaaagcg tgagttgatt
      301 gaagagcttg gtttcacagc agaaattggt acctattacg gacaagctga cgaatatttc
      361 tattctcgtc atcgtgatac ctactactac aatcctgcct acctctatga agcaacttct
      421 ttcaaagaag tacaaaagcc actagaaaac tttaatcata ttgcctggtt ccctattgac
      481 gaggctatcc aaaaccttaa acgtggtagc cataaatggg ccattgaatc ttggaaaaaa
      541 cagcataaga ttggctaata ctcttcgaaa ataaaattca aaccacgtca gcgtcgcctt
      601 accgtactca agtatagcct gcggctagct tcctagtttg ctctttgatt ttcattgagt
      661 ataaatcaag cgactttatt caaaaagtgc tataatagaa ttagaaaatc ggaggaaata
      721 ttatgaagct tatcaatacg acaaactcac actcgcaact ggtaaaaagt cagctagaaa
      781 gtaccgatgc aacccttgtt gaggtttatt ctgcagggaa tacagatgtt atttttaccc
      841 aagctccgct tcactatgaa atcctcatct caaacaaaca ccgtgctatt cgtgagcctg
      901 aaatcgaaac cattcaagaa tttttcttga aacgtaaaat tgataagacc tctgttgatg
      961 aagccaatat caagacactc tactcagaga aattaattgg aatttcaatc ccaatcaagt
     1021 aagaaaattg agtaaaaact cgatttgaga gaaagtccaa taagtctttc cataacaaaa
     1081 cgcatagtaa caagagtttc tacacccact attatgcgtt tttatcattt tgaaatctac
     1141 tgctaatcaa tgaagaagca aagatcaaac taggcagcta gttacaagtt a
//
LOCUS       DAVDWT010000086         1122 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVDWT010000086 DAVDWT010000000
VERSION     DAVDWT010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.086"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..954
                     /locus_tag="VUW50_002114"
     CDS             <1..954
                     /locus_tag="VUW50_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET7983930.1"
                     /translation="AKVESEKAEATRLEKIKTDRKKAEEEAKRKAAEEDKVKEKPAEQ
                     PQPAPAPQPEKPAEEPNPAPAPKPEKPAEQPKAEKTDDQQAEEDYARRSEEEYNRLTQ
                     QQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNANGAMATG
                     WLQNNGSWYYLNANGSMATGWLQNNGSWYYLNANGAMATGWLQYNGSWYYLNSNGAMA
                     TGWLQYNGSWYYLNANGDMATGWLQNNGSWYYLNANGAMVTGWVKDGDTWYYLEASGA
                     MKASQWFKVSDKWYYVNGSGALAVNTTVDSYRVNANGEWVN"
BASE COUNT          408 a          231 c          241 g          242 t
ORIGIN      
        1 gcgaaagttg agagtgaaaa agctgaggct acaaggttag aaaaaatcaa gacagatcgt
       61 aaaaaagcag aagaagaagc taaacgaaaa gcagcagaag aagataaagt taaagaaaaa
      121 ccagctgaac aaccacaacc agcgccggct cctcaaccag aaaaaccagc tgaagagcct
      181 aatccagctc cagctccaaa accagagaag ccagctgaac aaccaaaagc agaaaaaaca
      241 gatgatcaac aagctgaaga agactatgct cgtagatcag aagaagaata taatcgcttg
      301 actcaacagc aaccgccaaa aactgaaaaa ccagcacaac catctactcc gaaaacaggc
      361 tggaaacaag aaaacggtat gtggtacttc tacaatactg atggttcaat ggcaacaggt
      421 tggctccaaa acaacggttc atggtactat ctaaacgcta atggtgctat ggcgacagga
      481 tggctccaaa acaatggttc atggtactat ctaaacgcta atggttcaat ggcaacagga
      541 tggctccaaa acaatggttc atggtactac ctaaacgcta atggtgctat ggcgacagga
      601 tggctccaat acaatggttc atggtactac ctaaacagca atggcgctat ggcgacagga
      661 tggctccaat acaatggctc atggtactac ctcaacgcta atggtgatat ggcgacagga
      721 tggctccaaa acaacggttc atggtactac ctcaacgcta atggtgctat ggtgacaggt
      781 tgggtgaaag atggagatac ctggtactat cttgaagcat caggtgctat gaaagcaagc
      841 caatggttca aagtatcaga taaatggtac tatgtcaatg gctcaggtgc ccttgcagtc
      901 aacacaactg tagatagcta tagagtcaat gccaatggtg aatgggtaaa ctaaacttaa
      961 tataactagt taatactgac ttcctgtaag aactctttaa agtattccct acaaatacca
     1021 tatcctttca gtagataata tacccttgta ggaagtttag attaaaaaat aactctgtaa
     1081 tctctagccg gatttatagc gctagagact acggagtttt tt
//
LOCUS       DAVDWT010000087         1113 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVDWT010000087 DAVDWT010000000
VERSION     DAVDWT010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.087"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..359
                     /locus_tag="VUW50_002115"
     CDS             <1..359
                     /locus_tag="VUW50_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7983931.1"
                     /translation="KLLSYSQDLKHHYQLYQLLLFHFQNKKPEKFFGLIEDNLKQVHP
                     IFQTVFKTFLKNKEKIVNALQLPYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFI
                     TLNIKKERTKSVLSQA"
     gene            complement(401..488)
                     /locus_tag="VUW50_002116"
     tRNA            complement(401..488)
                     /locus_tag="VUW50_002116"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(452..454),aa:Ser,seq:gct)
     gene            complement(495..568)
                     /locus_tag="VUW50_002117"
     tRNA            complement(495..568)
                     /locus_tag="VUW50_002117"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(532..534),aa:Ile,seq:gat)
     gene            complement(604..674)
                     /locus_tag="VUW50_002118"
     tRNA            complement(604..674)
                     /locus_tag="VUW50_002118"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(640..642),aa:Gly,seq:tcc)
     gene            complement(694..766)
                     /locus_tag="VUW50_002119"
     tRNA            complement(694..766)
                     /locus_tag="VUW50_002119"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(731..733),aa:Phe,seq:gaa)
     gene            complement(770..843)
                     /locus_tag="VUW50_002120"
     tRNA            complement(770..843)
                     /locus_tag="VUW50_002120"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(807..809),aa:Met,seq:cat)
     gene            complement(854..943)
                     /locus_tag="VUW50_002121"
     tRNA            complement(854..943)
                     /locus_tag="VUW50_002121"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(905..907),aa:Ser,seq:tga)
     gene            complement(954..1027)
                     /locus_tag="VUW50_002122"
     tRNA            complement(954..1027)
                     /locus_tag="VUW50_002122"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(991..993),aa:Ile,seq:cat)
     gene            complement(1041..1113)
                     /locus_tag="VUW50_002123"
     tRNA            complement(1041..1113)
                     /locus_tag="VUW50_002123"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1078..1080),aa:Met,seq:cat)
BASE COUNT          337 a          295 c          219 g          262 t
ORIGIN      
        1 acaagctttt gagctattca caagacttga aacaccacta tcagctctat caactcttgc
       61 tgtttcactt tcagaataag aaaccggaga aatttttcgg actcattgag gacaatctga
      121 agcaggttca tcctattttt cagactgtct ttaaaacctt tctaaagaac aaagagaaaa
      181 tcgtcaacgc ccttcaacta ccctattcaa acgccaaatt ggaagcgacc aataatctca
      241 tcaaacttat caaacgcaat gcctttggtt ttcgaaactt tgaaaacttc aaaaaacgga
      301 tttttatcac tctgaacatc aaaaaagaaa ggacgaaatc tgtcctttct caagcttagc
      361 ttttcttcaa cccactacag ttgacaaaga gccattaaaa cggagaataa gggattcgaa
      421 cccttgcgcc agttacccga cctaacgatt tagcaaaccg tcctcttcag cctcttgagt
      481 aattctccaa ttaatgggca cgagtggact cgaaccaccg acctcacgct tatcaggcgt
      541 gcgctctaac cacctgagct acgcgcccaa gttaaaaaac ttggtaattt gaacaaagtt
      601 caaagcgggt gacgagaatc gaactcgcga caacagcttg gaaggctgta gttttaccac
      661 taaactacac ccgcataaat actatcaata aaatggcgcg agacggaatc gaaccgccga
      721 cacatggagc ttcaatccat tgctctacca actgagctac cgagccttat tgcgggagca
      781 ggatttgaac ctacgacctt cgggttatga gcccgacgag ctaccgagct gctccatccc
      841 gcgttaatat aaaaggagga tgtgggattc gaacccacgc acgcttttac acgcctgacg
      901 gttttcaaga ccgttccctt cagccggact tgggtaatcc tccaatattc aaatggacct
      961 tgtaggactt gaacctacga ccactcggtt atgagccgag agctctaacc agctgagcta
     1021 aaggtccgac aagatcatta tagcggcgaa ggggatcgaa cccccgacct cccgggtatg
     1081 aaccggacgc tctagccagc tgagctacac cgc
//
LOCUS       DAVDWT010000088         1046 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVDWT010000088 DAVDWT010000000
VERSION     DAVDWT010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.088"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..>1046
                     /locus_tag="VUW50_002124"
     CDS             <1..>1046
                     /locus_tag="VUW50_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HET7983932.1"
                     /translation="SSNPSDSTTSVGESNQPENNDSKNENSEKTREEVPVNPNEGTVE
                     GTSNQETENPVQPAEESTTNSEKVSPDTSSENTGEVSNKPSDSKPPVEESNQPEKNGT
                     ATKPENSGNTTSENGQTEPEPSNGNSTEDVSTKSNTSNSNGNEEIKQENELDPDKKVE
                     DPEKTLELRNVSDLELYSLSNGTYKQHISLEQVPSNPNSYFVKVKSSSFKDVYLPVAS
                     ISEERKNDKILYKITAKVEKLQQEIESRYKDNFTFYLAKKGTEETTNFTSFSNLVKAI
                     NQNLSGTYHLGASLNANEVELSTDDKSYIKGTFTGQLIGEKDGKHYAIYNLKKPLFES
                     LRGATIEKLSLKNVS"
BASE COUNT          447 a          189 c          178 g          232 t
ORIGIN      
        1 tccagtaatc ctagtgattc gacaacctca gttggagaat caaatcaacc agaaaataat
       61 gactctaaaa atgaaaattc agaaaaaact agagaagaag ttccagtaaa tccaaatgaa
      121 ggcacagtag aaggtacctc aaatcaagaa acagaaaatc cagttcaacc tgcagaagaa
      181 tcaacaacga attcagagaa agtatcacca gatacatcta gcgaaaatac tggggaagta
      241 tccaataaac ctagtgattc aaaaccacca gttgaagaat caaatcaacc agaaaaaaac
      301 ggaactgcaa caaaaccaga aaattcaggt aatacaacat cagagaatgg acaaacagaa
      361 ccagaaccat caaacggaaa ttcaactgag gatgtttcaa ccaaatcaaa cacatccaat
      421 tcaaatggaa acgaagaaat taaacaagaa aatgaactag accccgataa aaaggtagaa
      481 gatccagaga aaacacttga attaagaaat gtttccgacc tagagttata cagtttgtca
      541 aatggtactt ataaacaaca catttcgtta gaacaagttc caagcaatcc aaatagctac
      601 tttgttaaag tgaaatcttc ttcattcaaa gatgtatacc taccagtagc atcaatatca
      661 gaggaaagaa aaaatgataa aatcctttat aaaatcacag caaaagtaga gaagcttcag
      721 caggagatag aaagcagata taaagataat tttaccttct atctagctaa gaagggaaca
      781 gaagaaacaa caaactttac ttcctttagt aatctggtca aagctataaa ccaaaatctc
      841 tctggaacct atcatttagg agctagcctg aatgctaacg aagtggagct tagcactgat
      901 gataaatcct atatcaaggg aacctttact ggtcagttga ttggtgaaaa agatggtaag
      961 cattatgcta tttacaactt gaaaaagcct ctttttgaaa gcttgagagg ggccacaata
     1021 gaaaagttga gtctgaaaaa tgtctc
//
LOCUS       DAVDWT010000089          966 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVDWT010000089 DAVDWT010000000
VERSION     DAVDWT010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.089"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(258..569)
                     /locus_tag="VUW50_002125"
     CDS             complement(258..569)
                     /locus_tag="VUW50_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983933.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCLFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(584..943)
                     /locus_tag="VUW50_002126"
     CDS             complement(584..943)
                     /locus_tag="VUW50_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001840593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HET7983934.1"
                     /translation="MPSENEQLFGARRKIPEYKFVAEVVSYDDAVQTATIRQRNVINE
                     GDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELLTIKVPQPVQSGDMVRALKE
                     GLINFYKEDGTSVTVRA"
BASE COUNT          305 a          179 c          167 g          315 t
ORIGIN      
        1 agacggattg ttttgacaat tctaaccttt tagactttgc ataataactg ttaaacagtc
       61 gaaaattacc ccagttttaa caagtagtca aaataccaga taaagtaaaa aggggctagg
      121 aaaaaagtct ttaaaatctt aaaaacgcat agtatcaggt attgaacaat cttgatatta
      181 tgcgttttat tgtgggaaga tttacttcat tttctcttgg aattgagttt tctcctactt
      241 caaaactaga tgatttgcta tttaaaatag cgttccttat gattttggca acctggatta
      301 aagcgagaaa aacaaaagag gcaacttaag ctttctttat attgcttata tgttaatagt
      361 tttagacaaa caccacataa aataggctta tcctgtatca aagataaggg ataaggctca
      421 aaagggtgat tttctaatga atcatgacac cgataacaag cataatactt tttacaatca
      481 tagcattgta aagcaataat atccagcttg ctatggtaat gaatgcatct tgtttgctta
      541 tccactaaga aaccctgtgc ctctatcatt tccttttcct ttcttaagct cgaactgtga
      601 cgctggttcc atcttcctta taaaaattga tgagtccttc ttttaatgca cgaaccatgt
      661 ctcctgattg aacgggttgt gggactttaa tagtcaatag ttccattgga tttggagcgc
      721 ggtcgatttt atttcctttg gcatcatgca aatcttcaat ataggtttca aaatgacgga
      781 aacctggacc ataaaactca acttggtccc cttcgttaat gacgtttcgt tgacgaatgg
      841 ttgctgtttg taccgcatca tcataagaaa ccacttcagc gacaaacttg tactcaggaa
      901 ttttacggcg agcaccaaac aactgctcat tttcagatgg tataccgtag tagaaacctg
      961 ttgcca
//
LOCUS       DAVDWT010000090          874 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVDWT010000090 DAVDWT010000000
VERSION     DAVDWT010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.090"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            25..870
                     /locus_tag="VUW50_002127"
     CDS             25..870
                     /locus_tag="VUW50_002127"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET7983935.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKACAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
BASE COUNT          222 a          152 c          189 g          311 t
ORIGIN      
        1 taaatataat ataaggagaa atatatgtat cttattgaaa ttttaaaatc tatcttcttc
       61 gggattgttg aaggaattac ggaatggttg ccgatttcca gtacaggtca cttgatttta
      121 gcagaggagt ttatccaata ccaaaatcaa aatgaagcct ttatgtccat gtttaatgtc
      181 gtgattcagc ttggtgctat tttagcagtt atggtgattt attttaacaa gctcaatcct
      241 tttaaaccga ctaaggacaa acaggaagtt cgtaagactt ggagactatg gttgaaggtc
      301 ttgattgcta ctttaccttt acttggtgtc tttaaatttg atgattggtt tgatacccac
      361 ttccataaca tggtttcagt tgctctcatg ttgattatct acggggttgc cttcatctat
      421 ttggaaaagc gcaataaagc gtgtgctatc gagccaagtg taacagagtt ggacaagctt
      481 ccttatacga ccgctttcta tatcggactc ttccaagttc ttgctctttt accagggact
      541 agccgttcag gtgcaacgat tgtcggtggt ttgttaaatg gaaccagtcg ttcagttgtg
      601 acagaattta ccttctatct tgggattccc gttatgtttg gagctagtgc cttaaagatt
      661 ttcaaatttg tgaaagccgg agaactcttg agctttgggc aattgttttt gctcttggtc
      721 gcgatgggag tagcttttgc ggtcagcatg gtggctattc gcttcttgac cagctatgtg
      781 aaaaaacacg acttcaccct ttttggtaaa taccgtatcg tgcttggtag tgttttgcta
      841 ctttacagtt ttgtccgttt atttgtataa gaaa
//
LOCUS       DAVDWT010000091          813 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVDWT010000091 DAVDWT010000000
VERSION     DAVDWT010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.091"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(<1..>813)
                     /locus_tag="VUW50_002128"
     CDS             complement(<1..>813)
                     /locus_tag="VUW50_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET7983936.1"
                     /translation="ETVREMVSGGFRLDFLLETARLARSTYYYQLKQLDGVDKDKEIK
                     TEIQGIDNEHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLTARIRRKRKYSSYQ
                     GEIGKKAENLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPVLDGFNSEIIAYHL
                     STSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGN
                     SPDNSMMESFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNNKRIKVKL"
BASE COUNT          219 a          167 c          147 g          280 t
ORIGIN      
        1 ttagttttac cttaattcgt ttgttgttgt agtaatcaat gtagtccaca atagcttgtt
       61 caaggttttc taaagatcta aagttcttct catagccata aaacatttcg gattttaaaa
      121 tgccaaagaa agattccatc atgctgttgt ctgggctgtt gcccttgcgt gacatggatg
      181 cttgaattcc cttactctct aggaaccgat gataagaatc gtgttggtat tgccagcctt
      241 ggtcactatg gagaatcgta ttctcatagt atttctctgt aaaggcctgt tctaacatac
      301 tcttcacttg ttctaagttt ggcgaagtag aaagatgata agcaatgatt tcactgttaa
      361 aaccatctaa aactggtgat aaatagagtt tctgtgtgct atttggaatg gcaaactctg
      421 tcacatctgt atagcacttt tccattggcc tcgatgcttc gaactggcgt tgaatgagat
      481 tctctgcttt cttgccaatc tctccttggt aggaagaata cttccgttta cggcgaattc
      541 gagccgttaa accaagaatt ctcatcaacc gttgaacttt cttatgattg accacaaaac
      601 cacgattcct tagttctaaa tggattcgac gatagccata gttgccttta tgttcattat
      661 caatgccctg aatttcggtt ttaatctctt tatctttatc aaccccatcc agttgtttca
      721 actgataata gtaagtcgag cgagctaaac gtgctgtttc aagaagaaaa tctagtcgaa
      781 atcctcctga aaccatttct ctaactgtct ctg
//
LOCUS       DAVDWT010000092          801 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVDWT010000092 DAVDWT010000000
VERSION     DAVDWT010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.092"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..>801
                     /locus_tag="VUW50_002129"
     CDS             <1..>801
                     /locus_tag="VUW50_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HET7983937.1"
                     /translation="VKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKE
                     IREKILHYHKQKFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKK
                     QASPNFKPAGKSIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIR
                     LIPNKTAESVNQALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERG
                     SNENHNRLIRRWLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEF"
BASE COUNT          285 a          146 c          160 g          210 t
ORIGIN      
        1 aagtcaaaag aggtacaact ttacaacaag tgagaaaagg gctatacaaa aaggtctatt
       61 ctgccgatta cgcacaaact gtttaccaat tcaatcgaaa acggtcggtg aaaaagttaa
      121 ttttaacaaa ggaaatcaga gagaagatct tacactatca taagcaaaaa ttttcgcctg
      181 aaatgatggt taacaagaag caagtgaaag ttggtatttc aaccatctac tactggtttc
      241 ataatggtca tttaggattg acgaaggccg acatgcttta tcccagaaaa aggaaaggtg
      301 tcaagaagca agctagtccg aactttaagc cggcaggtaa atctatcgaa gaacgtcctg
      361 acgttattaa tcttcgcttg gaaaatggtc attatgaaat tgataccgtc ctactgacta
      421 agataaaaaa ttattgcctg ttagtcttaa ccgaccggcg gagcagacac caaattataa
      481 ggttaattcc aaataaaact gctgaatctg tcaatcaggc gcttacgtta ctattagggg
      541 agcatcgtat tctgtccatt actgcagata atggttcgga gttcaaacga ttgtctgagg
      601 tatttcctga ggaacatatc tactacgcac atgcttactc ttcatgggag agaggttcaa
      661 atgaaaatca taatcgatta attcggagat ggttacctaa aggaaccaag aaaacgactc
      721 cgaaagaagt agcttttatc gaaaattgga ttaacaacta ccctaaaaaa tgcttggact
      781 acaagtcgcc aagtgaattt c
//
LOCUS       DAVDWT010000093          776 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVDWT010000093 DAVDWT010000000
VERSION     DAVDWT010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.093"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..>776
                     /locus_tag="VUW50_002130"
     CDS             <1..>776
                     /locus_tag="VUW50_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET7983938.1"
                     /translation="DGSTQEIDVPYDIPDTWEWVRFSTLVEIVRGGSPRPIKDYLTSE
                     VDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLTNSMSFGRPYILNV
                     DGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILI
                     PLPPLSEQQRIVEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVE
                     QDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQ"
BASE COUNT          294 a          107 c          138 g          237 t
ORIGIN      
        1 tgatggaagc actcaagaaa ttgatgttcc ttatgatatt cctgatactt gggagtgggt
       61 gaggttttct acattggttg aaattgtcag aggtggctct ccacgaccaa tcaaagatta
      121 tcttacttct gaagtagatg gaataaattg gataaaaata ggtgatactg aaaagggtga
      181 aaagtatata aataatgtta aagaaaaaat caaaaaatca gggcttaaca aaactagatt
      241 tgtaaaaaaa ggtacatttt tgttaactaa ttctatgagt tttggtagac cttatatttt
      301 gaatgttgat ggtgcaatac acgatggatg gttggctatt tcgaactatg aaaactcatt
      361 aaataaagat tacctattct atattctttc atcaaatgta gtttattctc aatttctatc
      421 tctaattagt ggagctgttg tgaaaaactt gaatagtgat aaagttgctt ctattcttat
      481 ccctctcccc ccactatccg aacaacaacg aatagtagaa gcaatcgaat cagctttaga
      541 aaaagtagat gaatatgctg aaagttataa tagactagaa cagctagata aagaatttcc
      601 agataaacta aaaaaatcta ttcttcaata tgctatgcaa ggaaaattag ttgaacaaga
      661 cccaaatgat gaatcagtcg aagttttact tgaaaaaata cgagcagaaa aacaaaaact
      721 ctttgaagaa ggcaagatta aaaagaaaga tttggacatt tctattgttt cccaag
//
LOCUS       DAVDWT010000094          692 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVDWT010000094 DAVDWT010000000
VERSION     DAVDWT010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.094"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(74..147)
                     /locus_tag="VUW50_002131"
     tRNA            complement(74..147)
                     /locus_tag="VUW50_002131"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Arg,seq:acg)
     gene            complement(157..242)
                     /locus_tag="VUW50_002132"
     tRNA            complement(157..242)
                     /locus_tag="VUW50_002132"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(206..208),aa:Leu,seq:taa)
     gene            complement(250..321)
                     /locus_tag="VUW50_002133"
     tRNA            complement(250..321)
                     /locus_tag="VUW50_002133"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Gly,seq:gcc)
     gene            complement(344..416)
                     /locus_tag="VUW50_002134"
     tRNA            complement(344..416)
                     /locus_tag="VUW50_002134"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(381..383),aa:Thr,seq:tgt)
     gene            complement(435..516)
                     /locus_tag="VUW50_002135"
     tRNA            complement(435..516)
                     /locus_tag="VUW50_002135"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(480..482),aa:Leu,seq:tag)
     gene            complement(522..594)
                     /locus_tag="VUW50_002136"
     tRNA            complement(522..594)
                     /locus_tag="VUW50_002136"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(559..561),aa:Lys,seq:ttt)
     gene            complement(619..691)
                     /locus_tag="VUW50_002137"
     tRNA            complement(619..691)
                     /locus_tag="VUW50_002137"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(656..658),aa:Asp,seq:gtc)
BASE COUNT          179 a          204 c          149 g          160 t
ORIGIN      
        1 cgaacctgcg acaccttggt cccaaaccaa gtactctacc aagctgagct acttcccgag
       61 ttaaatagaa aaatgcaccc tagaggagtc gaacctctaa ccgcctgatt cgtagtcagg
      121 tactctatcc agttgagcta agggtgctcc atattatgcc gaggaccgga atcgaaccgg
      181 tacgatcgtt accaatcgca ggattttaag tcctgtgcgt ctgccagttc cgccaccccg
      241 gcctctctaa gcgaacgacg ggattcgaac ccgcgacccc caccttggca aggtggtgtt
      301 ctaccactga actacgttcg cactgttttc ttctatctaa aaatgccggc tacatgactt
      361 gaacacgcga ccctctgatt acaaatcaga tgctctacca actgagctaa gccggctcat
      421 ttgttatatc ttaatgcggg ttaagggact tgaaccccca cgccgttaag cgccagatcc
      481 taaatctggt gcgtctgcca attccgccaa acccgcatat atgacccgta ctgggctcga
      541 accagtgacc cattgattaa aagtcaattg ctctaccaac tgagctaacg agtctaaaat
      601 aacttgcgtt atcttaaacg gtcccgacgg gaatcgaacc cgcgatcttc gccgtgacag
      661 ggcgacgtga taaccgctac actacgggac ct
//
LOCUS       DAVDWT010000095          666 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVDWT010000095 DAVDWT010000000
VERSION     DAVDWT010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.095"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            1..666
                     /locus_tag="VUW50_002138"
                     /pseudo
     CDS             1..666
                     /locus_tag="VUW50_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          194 a          141 c          128 g          203 t
ORIGIN      
        1 attgctcaaa agttaattga aaagatttct atgaccgata ttgcccatca gctttccatc
       61 tcaacttcaa ctgtcattcg aaagctcaat gacttccact ttgagtgtaa ttttagaaat
      121 ctgcctaaga ttatgtcttg ggacgttgaa acagtccggg gagtgactgt ttcaatcggg
      181 agatggagat gagctttatt gcgcaagatt ttgaaaagct cgatatcatc actgttcttg
      241 aaggtagaac acaagctgcc atccgagatc actttcttaa atatgataga gccgtccgat
      301 gtcgcgtcaa aatcattact atggatatgt ttagccctta ctatgacttg gctaaacagc
      361 ttcgctttca aatttctagg ctcaggctga aacagtctcc cagactgttt cactcccgaa
      421 tgctgaaatc gttcttgatc gctttcacat tgtacaacat cttagccgtg ctatgagtcg
      481 tgtgcgtgtt caaatcatga atcagtttca tcgaaaatcc catgaataca aggctatcaa
      541 gcgctactgg aagctcatac aacaggatag ccgtaaactg agtgataagc gattttatcg
      601 tcctactttt cgtatgcact taaccaataa agagatttta gacaagcttt tgagctattc
      661 acaaga
//
LOCUS       DAVDWT010000096          590 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVDWT010000096 DAVDWT010000000
VERSION     DAVDWT010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.096"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(7..486)
                     /locus_tag="VUW50_002139"
     CDS             complement(7..486)
                     /locus_tag="VUW50_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7983939.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRKYDKEPYEEVGELSHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
BASE COUNT          159 a          139 c           80 g          212 t
ORIGIN      
        1 gacttcctag tgggtacgga tagtaaactc cttaaacaca atgcgtaagt ctcttaatac
       61 tctttgacgc cctcgaaatc gttcattgct taagacacgt tctagttctt cttgtttctc
      121 tttactttgt ttgtttctat aatctcttag tgtttcatga aagagataat aatcatctag
      181 ccagagaccc ccctcactta tacgatgact gagctcaccc acttcttcat agggttcttt
      241 atcgtattta cgcttctgac tttcctgctt acggatatag tctaaaattc gatttcgaaa
      301 cttggttttg aaatatttcc ttaaacgtgg aatatcgtct accagtcctt cttctctact
      361 aatcaattca tgtaagcaga gcatgccttc ttggtcccaa tccgataatt cccataaatg
      421 aaggtaatat tcatttctac acttatacac agtcccttgg acttcttcat acaattcttt
      481 aatcataatt ttcccctttc tagatacagt ctaacagatt agaaaacaca tttgttccat
      541 tttctccatt ctgttccctt tagctcctaa actgcacaaa aaagagaggc
//
LOCUS       DAVDWT010000097          485 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVDWT010000097 DAVDWT010000000
VERSION     DAVDWT010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.097"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            1..343
                     /locus_tag="VUW50_002140"
                     /pseudo
     CDS             1..343
                     /locus_tag="VUW50_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          160 a           78 c           93 g          154 t
ORIGIN      
        1 ggattcgata cttattttta tcgagaatat ggtcgctcat taaaaggtca gttaataaga
       61 ggcaaagtat ctggaagaag atatcagaga atttctttgg ttgcaggtct aacaaatgaa
      121 tggtgagtta atcgctccaa tgacttacga agagacgatg acgagcgact tttttgaagc
      181 ttggtttcag aagtttctct taccaacatt aaccacacca tcagttatta ttatggataa
      241 tgcaagattc catagaatgg gtaagttaga actcttgtgt gaagagtttg ggtataaact
      301 tttacctctt cctccctact cacctgagta caatcctatt gaggctctat aatatttgta
      361 gtgggtaaat cccctatgga tattatggag cctatttttg tgtagaaaaa aagtcccata
      421 agatctataa tgaaaagcga tcaaacaact cattagaaag attcatatgg aacaattaca
      481 tttta
//
LOCUS       DAVDWT010000098          473 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVDWT010000098 DAVDWT010000000
VERSION     DAVDWT010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.098"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..>473
                     /locus_tag="VUW50_002141"
     CDS             <1..>473
                     /locus_tag="VUW50_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET7983940.1"
                     /translation="NKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLNTKNVTK
                     NGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHLRINSGM
                     VILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLALQN"
BASE COUNT          186 a           72 c           73 g          142 t
ORIGIN      
        1 aataaaatat ttgaaagcat tgataactta tttgatatta tagatggtga taggggcaaa
       61 aattatccta aatcagatga gttgtttagt gaggagtact gtttattttt aaatacaaag
      121 aatgttacta aaaacggatt ttcattcgat acaaagcaat ttatcactaa aacaaaggat
      181 aaattacttc gaaaaggcaa acttgagcgt tatgatatag tcttgacaac aagaggtact
      241 gttggaaatg tagcgtacta cgatgaatta ataaaatata aacatttacg tataaattca
      301 ggtatggtaa tattacgtcc caagacacca aatctaaatc agaaatttat tatccatgtt
      361 ttaaggaata ataattatag tcgagtgata tcaggaagtg ctcagcctca gttaccaatt
      421 acaaaattaa aaaaaatact tctccccctc cccccactag ccctccaaaa tga
//
LOCUS       DAVDWT010000099          416 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVDWT010000099 DAVDWT010000000
VERSION     DAVDWT010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.099"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..>416
                     /locus_tag="VUW50_002142"
     CDS             <1..>416
                     /locus_tag="VUW50_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET7983941.1"
                     /translation="PLNNNKKFAVKTGQQCFKFSSGKFLDKHDRVFEGYPAYGGNGIA
                     WKSRKYLIDNPTIIIGRVGAYCGNVRTTHGKVWISDNAIYIKEFKNSDFNLVFLLELM
                     KVIDFSKFADFSGQPKITQKPLENQKYILPPLALQN"
BASE COUNT          152 a           60 c           70 g          134 t
ORIGIN      
        1 cctttaaata ataacaagaa atttgcagta aaaacgggac aacagtgttt taaattttca
       61 agtggaaaat ttcttgataa acatgataga gtttttgaag gttatcctgc ctatggtgga
      121 aatggcattg cttggaaaag tcgaaaatat ttaattgata atcctactat catcattggt
      181 cgtgtaggtg catattgtgg taatgtaaga actacccacg gtaaggtatg gataagtgac
      241 aatgctatat atataaaaga atttaagaat tccgatttca atcttgtatt tttattagaa
      301 ttaatgaaag taattgattt tagtaaattt gctgatttct caggtcaacc aaagataact
      361 caaaaaccat tagaaaatca gaaatatatc ctcccccctc tagccctcca aaatga
//
LOCUS       DAVDWT010000100          404 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVDWT010000100 DAVDWT010000000
VERSION     DAVDWT010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.100"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            complement(<1..>404)
                     /locus_tag="VUW50_002143"
     CDS             complement(<1..>404)
                     /locus_tag="VUW50_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7983942.1"
                     /translation="KIMSWDEYAFTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFL
                     RYDRAVRCQVKIITMDMFSPYYDLAKQLFPCAKIVLDRFHIIQHLSRAMSRFRVQIMN
                     QFERKSHEYKAIKRYWKLIQQDSRKLSDKRFYR"
BASE COUNT          131 a           77 c           76 g          120 t
ORIGIN      
        1 cgataaaaac gtttatcgct caatttacga ctatcctgtt ggatgagttt ccagtaacgt
       61 ttgatagcct tgtattcatg agattttcgt tcaaactgat tcataatttg aacacgaaaa
      121 cgactcatgg cacggctgag atgttggata atatggaaac gatctagaac gattttagca
      181 cacggaaaaa gctgtttagc caagtcatag taaggactaa acatatccat cgtaatgatt
      241 ttcacttgac aacgaacggc tctatcgtag cgaagaaagt gatttcggat gacagcttgt
      301 gttctgcctt caagaacagt gataatatta agattatcaa aatcttgcgc aatgaaactc
      361 atctttccct tagtgaaggc atactcatcc caagacataa tctt
//
LOCUS       DAVDWT010000101          399 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVDWT010000101 DAVDWT010000000
VERSION     DAVDWT010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.101"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..>399
                     /locus_tag="VUW50_002144"
     CDS             <1..>399
                     /locus_tag="VUW50_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HET7983943.1"
                     /translation="LSPLGENVKTKGQYFYQVALDGNVVGKEKQALIDQFRANGTQTY
                     SATVNVYGNKDGKPDLDNIVATKKVTININGLISKETVQKAVADNVKDSIDVPAAYLE
                     KAKGEGPFTAGVNHVIPYELFAGDGMLTRL"
BASE COUNT          139 a           86 c           81 g           93 t
ORIGIN      
        1 tctatctcca ctaggtgaaa acgtgaagac caaaggtcaa tacttctatc aagtagcctt
       61 ggacggaaat gtagttggca aagaaaaaca agcgctcatt gaccagttcc gagcaaatgg
      121 tactcaaact tacagcgcta cagtcaatgt ctatggtaac aaagacggta aaccagactt
      181 ggacaacatc gtagcaacta aaaaagtcac tattaacata aacggtttaa tttctaaaga
      241 aacagttcaa aaagccgttg cagacaacgt taaagacagt atcgatgttc cagcagccta
      301 cctagaaaaa gccaagggtg aaggtccatt cacagcaggt gtcaaccatg tgattccata
      361 cgaactcttc gcaggtgatg gtatgttgac tcgtctctt
//
LOCUS       DAVDWT010000102          382 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVDWT010000102 DAVDWT010000000
VERSION     DAVDWT010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.102"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..>382
                     /locus_tag="VUW50_002145"
     CDS             <1..>382
                     /locus_tag="VUW50_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7983944.1"
                     /translation="RKPKHAIPDEIKERILKKYLSYETYKPNVLHFCELLAEEEGIKL
                     SDTTVRKILYKKNILSPKSHRKTKKRVRKQAKLNLNQPLDNPILPTAKDFLEDPKKVH
                     PSRPRKKFAGELIQMDASPHAWFGP"
BASE COUNT          150 a           87 c           62 g           83 t
ORIGIN      
        1 cgaaaaccaa aacatgcaat ccctgatgaa atcaaagaac gtatcctaaa gaaatacctc
       61 tcctatgaaa catataaacc aaatgtcctt catttctgtg aattactagc tgaagaagag
      121 ggaattaagc tctctgatac aactgttaga aaaatactct ataagaaaaa catcctttct
      181 cctaagtctc acagaaagac aaagaagaga gtaagaaaac aagctaaact gaacctgaat
      241 caacccctag acaatccaat cttaccgacg gctaaagact tcctggaaga ccctaaaaag
      301 gtacatccta gtagacccag aaagaaattt gctggagaac tcattcaaat ggatgccagc
      361 cctcatgcct ggtttggacc ag
//
LOCUS       DAVDWT010000103          364 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVDWT010000103 DAVDWT010000000
VERSION     DAVDWT010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.103"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..>364
                     /locus_tag="VUW50_002146"
     CDS             <1..>364
                     /locus_tag="VUW50_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET7983945.1"
                     /translation="DDILAFLAPIRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDG
                     YIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQD
                     SGNTEAKGAEAIYNRVKAA"
BASE COUNT          117 a           79 c           85 g           83 t
ORIGIN      
        1 tggatgatat tcttgccttc ttagctccga ttcgtcatcc agaacgttta ggaaaaccaa
       61 atgcgcaaat tacctacact gatgatgaga ttcaagtagc caagttggca ggcaagtaca
      121 caacagaaga cggttatatc tttgatcctc gtgatataac cagtgatgag ggggatgcct
      181 atgtaactcc acatatgacc catagccact ggattaaaaa agatagtttg tctgaagctg
      241 agagagcggc agcccaggct tatgctaaag agaaaggttt gacccctcct tcgacagacc
      301 atcaggattc aggaaatact gaggcaaaag gagcagaagc tatctacaac cgcgtgaaag
      361 cagc
//
LOCUS       DAVDWT010000104          351 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVDWT010000104 DAVDWT010000000
VERSION     DAVDWT010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.104"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..>351
                     /locus_tag="VUW50_002147"
     CDS             <1..>351
                     /locus_tag="VUW50_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HET7983946.1"
                     /translation="RLEEIKTDREEAKRKADAKLKEAVEKNAANSEQGEPKRRVKRGV
                     LGEPATPDKKENDAKSSDSSVGEETLPSPSLKPEKKVAEAEKKVEEAKKKAEDQKEED
                     RRNYPTNTYKTLEL"
BASE COUNT          148 a           60 c           87 g           56 t
ORIGIN      
        1 aaggttagaa gaaatcaaga cagatcgtga agaagctaaa cgaaaagcag atgctaagtt
       61 gaaggaagct gttgaaaaga atgcagcgaa ttcagagcaa ggtgaaccaa agaggcgggt
      121 aaaacgagga gttcttggag agccagcaac acctgataaa aaagaaaatg atgcgaagtc
      181 ttcagattct agcgtaggtg aagaaactct tccaagccca tccctgaaac cagaaaaaaa
      241 ggttgcagaa gctgagaaga aggttgaaga agctaagaaa aaagccgagg atcaaaaaga
      301 agaagatcgc cgtaactacc caaccaatac ttacaaaacg cttgaacttg a
//
LOCUS       DAVDWT010000105          326 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVDWT010000105 DAVDWT010000000
VERSION     DAVDWT010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.105"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            231..>326
                     /locus_tag="VUW50_002148"
     CDS             231..>326
                     /locus_tag="VUW50_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7983947.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNRE"
BASE COUNT          113 a           51 c           72 g           90 t
ORIGIN      
        1 taccttagtc aagaattaga tgtttcacta tgtttgagta agttgatgat ttcatttgat
       61 aacaggtttg aaactgttag gctaggtggc caaggctaat catagccttg gtttagctga
      121 aaaaacaggt tcaagggttc ctgttgtcaa atgaaatgtg atttaaggta taagaaaaca
      181 cctctgtgct atacttgttg ttcaccacaa acacaaggaa aggcacagag atgcaagaac
      241 attatacccc aaaagggaaa catttgacaa tagataaccg tcgcttgatt gagcggtgga
      301 agaatgaaaa taagtccaat cgtgaa
//
LOCUS       DAVDWT010000106          319 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVDWT010000106 DAVDWT010000000
VERSION     DAVDWT010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.106"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..>319
                     /locus_tag="VUW50_002149"
     CDS             <1..>319
                     /locus_tag="VUW50_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7983948.1"
                     /translation="PAPSNPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETA
                     AGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKAYDLLARIHQDLLDNKGRQVDFEA
                     LDNL"
BASE COUNT          107 a           58 c           71 g           83 t
ORIGIN      
        1 accagctcca agcaatccaa ttgatgagaa attggtcaaa gaagctgttc gaaaagtagg
       61 cgatggttat gtctttgagg agaatggagt ttctcgttat atcccagcca aggatctttc
      121 agcagaaaca gcagcaggca ttgatagcaa actggccaag caggaaagtt tatctcataa
      181 gctaggagct aagaaaactg acctcccatc tagtgatcga gaattttaca ataaggctta
      241 tgacttacta gcaagaattc accaagattt acttgataat aaaggtcgac aagttgattt
      301 tgaggctttg gataacctg
//
LOCUS       DAVDWT010000107          317 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVDWT010000107 DAVDWT010000000
VERSION     DAVDWT010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.107"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            15..>317
                     /locus_tag="VUW50_002150"
     CDS             15..>317
                     /locus_tag="VUW50_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7983949.1"
                     /translation="MHEQGYKVYTYNPLLIKKFAKSLSLRKTKTDKKDAHGIALKLLS
                     DPNREQFQHDNRQVELKILARHIHRLKKKQSDWKVQYTRCLDIIFPELDKIVGKHSE"
BASE COUNT          114 a           68 c           48 g           87 t
ORIGIN      
        1 atctactgaa cttcctgcat gaacaaggat ataaagttta cacctacaat cctttgctca
       61 tcaagaaatt tgccaaatca ttatcacttc gcaaaactaa aacggataag aaagacgctc
      121 atggcatcgc tcttaaactt ctttctgacc ccaatcgtga acaatttcaa cacgataatc
      181 ggcaagtaga actaaaaata ctggctagac atattcatcg tctcaagaaa aaacagtctg
      241 attggaaagt acaatacact cgttgtcttg atatcatctt tcctgagttg gataaaatcg
      301 ttggaaagca ttcagaa
//
LOCUS       DAVDWT010000108          311 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVDWT010000108 DAVDWT010000000
VERSION     DAVDWT010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.108"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..>311
                     /locus_tag="VUW50_002151"
     CDS             <1..>311
                     /locus_tag="VUW50_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7983950.1"
                     /translation="IINMDTHKEIIAKLDYEAPSCPDCGSLMKKYDFQKPSKIPYLET
                     TGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQIPRIINQKIAQKLIEKISMTDIA
                     H"
BASE COUNT          108 a           69 c           48 g           86 t
ORIGIN      
        1 atcatcaata tggataccca caaagaaatt atcgctaagc tggattatga ggctccatct
       61 tgccctgatt gtggaagtct aatgaagaaa tatgactttc aaaaaccgtc taagatccct
      121 tacctcgaaa caactggtat gccttctaga attctcctta gaaaacgccg tttcaagtgc
      181 tatcactgtt caaaaatgat ggttgctgag actcccctgg taaagaaaaa tcaccaaatc
      241 cctcgtatca tcaaccaaaa aattgctcaa aagttaattg aaaaaatttc tatgactgat
      301 attgcccatc a
//
LOCUS       DAVDWT010000109          304 bp    DNA     linear   BCT 16-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 398
            SAMEA2446324-rid19722613.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVDWT010000109 DAVDWT010000000
VERSION     DAVDWT010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2446324
            Sequence Read Archive: ERR540661
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001929651.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001929651.1
            Genome Coverage       :: 188x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2023 04:30:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,107
            Genes (coding)                    :: 1,968
            CDSs (with protein)               :: 1,968
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 74 of 139
            Pseudo Genes (incomplete)         :: 52 of 139
            Pseudo Genes (internal stop)      :: 45 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2446324-rid19722613.denovo.109"
                     /strain="398"
                     /db_xref="taxon:1313"
     gene            <1..>304
                     /locus_tag="VUW50_002152"
     CDS             <1..>304
                     /locus_tag="VUW50_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET7983951.1"
                     /translation="GGCSQSCRWKYDLYDMPFGKERKSLQGEIPEEFSMSAVDMSMID
                     HIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAYLESSEKFEAIKQDLVDEM"
BASE COUNT           94 a           52 c           75 g           83 t
ORIGIN      
        1 tggtggatgt tctcagtcat gccgttggaa atacgacctt tacgatatgc catttgggaa
       61 agaacgtaag agtttgcagg gtgagattcc agaagaattt tcaatgtcag ccgttgacat
      121 gtctatgatt gaccacattc cagatatgat tgaaaatggt gtggacagtc taaaaatcga
      181 aggacgtatg aagtctattc actacgtatc aacagtaacc aactgctaca aggcggctgt
      241 ggatgcctat cttgaaagtt ctgaaaagtt tgaagctatc aaacaagact tggtggacga
      301 gatg
//
